title: Transcriptome-Wide Mapping of N1-Methyladenosine Methylome.
abstract: N1-Methyladenosine (m1A) is a prevalent posttranscriptional RNA modification and commonly found in tRNA and rRNA. Very recent works have also demonstrated the prevalence of m1A in mammalian mRNA. Hence, high-throughput methods that allow transcriptome-wide mapping of m1A will be important for further functional investigations. Here, we describe a technique called "m1A-ID-Seq", which is based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, to map m1A in the transcriptome. Utilizing this technique, highly confident m1A peaks can be obtained on a transcriptome-wide level.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. chengqi.yi@pku.edu.cn.|Department of Chemical Biology, Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. chengqi.yi@pku.edu.cn.
authors.forename: Chengqi
authors.initials: C
authors.lastname: Yi
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000071496:AlkB Enzymes / Q000378:metabolism; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000379:methods*; D015723:Gene Library; D047468:Immunoprecipitation / Q000379:methods; D008745:Methylation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; C002230:1-methyladenosine; D000071496:AlkB Enzymes; D000241:Adenosine
keywords: AlkB; Demethylase; Epitranscriptome; N 1-Methyladenosine (m1A); RNA epigenetics; Transcriptome-wide profiling; m1A-ID-Seq