title: Special AT-rich sequence binding protein 1 promotes tumor growth and metastasis of esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma is one of the most aggressive malignancies worldwide. Special AT-rich sequence binding protein 1 is a nuclear matrix attachment region binding protein which participates in higher order chromatin organization and tissue-specific gene expression. However, the role of special AT-rich sequence binding protein 1 in esophageal squamous cell carcinoma remains unknown. In this study, western blot and quantitative real-time polymerase chain reaction analysis were performed to identify differentially expressed special AT-rich sequence binding protein 1 in a series of esophageal squamous cell carcinoma tissue samples. The effects of special AT-rich sequence binding protein 1 silencing by two short-hairpin RNAs on cell proliferation, migration, and invasion were assessed by the CCK-8 assay and transwell assays in esophageal squamous cell carcinoma in vitro. Special AT-rich sequence binding protein 1 was significantly upregulated in esophageal squamous cell carcinoma tissue samples and cell lines. Silencing of special AT-rich sequence binding protein 1 inhibited the proliferation of KYSE450 and EC9706 cells which have a relatively high level of special AT-rich sequence binding protein 1, and the ability of migration and invasion of KYSE450 and EC9706 cells was distinctly suppressed. Special AT-rich sequence binding protein 1 could be a potential target for the treatment of esophageal squamous cell carcinoma and inhibition of special AT-rich sequence binding protein 1 may provide a new strategy for the prevention of esophageal squamous cell carcinoma invasion and metastasis.
authors.affiliation: 1 Department of Thoracic Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: 2 Department of Colorectal Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Wu
authors.affiliation: 3 Department of Pancreato-Biliary Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
authors.forename: Zhenxian
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: 4 Operation Centre, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Miao
authors.affiliation: 5 Division of Cardiac Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Xu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D036961:Matrix Attachment Region Binding Proteins; D034741:RNA, Small Interfering; C076189:SATB1 protein, human
keywords: Esophageal squamous cell carcinoma; migration; proliferation; special AT-rich sequence binding protein 1; targeted therapy