title: Efficacy of soluble glycoprotein fraction from Allium sativum purified by size exclusion chromatography on murine Schistosomiasis mansoni.
abstract: In this work, the efficiency of crude MeOH extracts and soluble glycoprotein fraction of Allium sativum purified by size-exclusion chromatography (SEC) on parasitological, histopathological and some biochemical parameters in Schistosoma mansoni infected mice were investigated. Animals were infected by tail immersion with 100 cercariae/each mouse and divided into five groups in addition to the normal control. The results revealed a significant decrease in mean worm burden in all treated mice especially in the group treated with soluble glycoprotein fraction of A. sativum as compared to infected non-treated control with the disappearance of female worms. Administration of the studied extracts revealed remarkable amelioration in the levels of all the measured parameters in S. mansoni infected mice. In addition, treatment of mice with crude A. sativum MeOH extract and soluble glycoprotein fraction of A. sativum decreased significantly the activities of studied enzymes as compared to the infected untreated group. The highest degrees of enhancement in pathological changes was observed in the treated one with soluble glycoprotein fraction of A. sativum compared to the infected group represented by small sized, late fibro-cellular granuloma, the decrease in cellular constituents and degenerative changes in eggs. In conclusion, A. sativum treatment had effective schistosomicidal activities, through reduction of worm burden and tissue eggs, especially when it was given in purified glycoprotein fraction. Moreover, the soluble glycoprotein fraction of A. sativum largely modulates both the size and the number of granulomas.
authors.affiliation: Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aly
authors.affiliation: Clinical Parasitology Department, Research Institute of Ophthalmology, Giza, Egypt.
authors.forename: Eman E
authors.initials: EE
authors.lastname: Taher
authors.affiliation: Ph.D. of Immunology and Parasitology, Egypt.
authors.forename: Hoda
authors.initials: H
authors.lastname: El-Sayed
authors.affiliation: Parasitology Department, Faculty of Medicine, Zagazig University, Egypt.
authors.forename: Faten A
authors.initials: FA
authors.lastname: Mohammed
authors.affiliation: Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt; Natural Sciences, Math and Science Program, University College, Abu Dhabi University, United Arab Emirates.
authors.forename: Gehan
authors.initials: G
authors.lastname: ELnain
authors.affiliation: Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt; Biological Sciences Department, King Faisal University, Saudi Arabia.
authors.forename: Rabab S
authors.initials: RS
authors.lastname: Hamad
authors.affiliation: Hydrobiology Department, National Research Centre, Dokki, Giza, Egypt; Biology Dept., Girls Science College, University of Dammam, Saudi Arabia. Electronic address: bayoumy2004@yahoo.com.
authors.forename: Elsayed M
authors.initials: EM
authors.lastname: Bayoumy
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D005737:Garlic / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000494:pharmacology*; D006099:Granuloma / Q000469:parasitology / Q000473:pathology; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008297:Male; D051379:Mice; D010270:Parasite Egg Count; D010936:Plant Extracts / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000472:pathogenicity; D012555:Schistosomiasis mansoni / Q000097:blood / Q000188:drug therapy* / Q000469:parasitology / Q000473:pathology; D012556:Schistosomicides / Q000494:pharmacology; D044967:Serum / Q000737:chemistry; D000637:Transaminases / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D006023:Glycoproteins; D010936:Plant Extracts; D012556:Schistosomicides; D005723:gamma-Glutamyltransferase; D000637:Transaminases; D000469:Alkaline Phosphatase
keywords: Allium sativum; Fibrocellular granuloma; Schistosoma mansoni; Soluble glycoprotein fraction