title: Identification of bacteria using volatile organic compounds.
abstract: The rapid diagnosis of respiratory infections has always been an important goal for medical professionals, because rapid and accurate diagnosis leads to proper and timely treatment, and consequently, reduces the costs of incorrect and long-term treatments, and antibiotic resistance. The present study was conducted with the aim of detecting volatile organic compounds (VOCs) in three bacteria: Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae. Headspace of the studied bacteria, after separately culturing in two types of liquid medium in three different time-periods, was extracted by solid phase microextraction and analysed by gas chromatography mass spectrometry The analysis results of the VOCs produced by the studied bacteria indicate that some VOCs are common and some are unique in each bacterium. 1-penten-3-ol, levomenthol, and 2-octyl-1-ol for P. aeruginosa, cyclohexene, 4-ethenyl, and cis-Dihydro-α-terpinyl acetate for A. baumannii and 1,3-butadiene, butyraldehyde, longifolene, octyl acetate, tridecanol, dodecenal, (E)-2-hexyl ester, butanoic acid, and 5,5-dodecadinyl-1 12-diol for K. pneumoniae were identified as unique VOCs for each bacterium. Finally, it can be said that an accurate and rapid bacterial detection method can be achieved by using a tool that can detect bacterial VOCs. However, more studies are needed to design a tool for which all aspects have been assessed, so that it can give us a more complete pattern for the use of these compounds as biomarkers.
authors.affiliation: Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Karami
authors.affiliation: Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Karimi
authors.affiliation: Department of Biology, Medicinal Plant and Drug Research Institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Aliahmadi
authors.affiliation: Department of Biotechnology, The Faculty of Renewable Energies & New Technologies Engineering (NTE), Shahid Beheshti University, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Mirzajan
authors.affiliation: Department of Phytochemsitry, Medicinal plants and Drugs research Institute, ShahidBeheshti University, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Rezadoost
authors.affiliation: Department of Phytochemsitry, Medicinal plants and Drugs research Institute, ShahidBeheshti University, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Ghassempour
authors.affiliation: Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Fallah
mesh_terms: D000085:Acetates / Q000032:analysis / Q000302:isolation & purification; D040981:Acinetobacter baumannii / Q000737:chemistry*; D000447:Aldehydes / Q000032:analysis / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D002070:Butadienes / Q000032:analysis / Q000302:isolation & purification; D020148:Butyric Acid / Q000032:analysis / Q000302:isolation & purification; D053138:Cyclohexenes / Q000032:analysis / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D000439:Pentanols / Q000032:analysis / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D012137:Respiratory System / Q000382:microbiology; D012717:Sesquiterpenes / Q000032:analysis / Q000302:isolation & purification; D052617:Solid Phase Microextraction; D013045:Species Specificity; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000085:Acetates; D000447:Aldehydes; D002070:Butadienes; D053138:Cyclohexenes; D000439:Pentanols; D012717:Sesquiterpenes; D055549:Volatile Organic Compounds; D020148:Butyric Acid; C052568:cyclohexene; C035607:longifolene; C068720:1-penten-3-ol; C018475:butyraldehyde; C031763:1,3-butadiene; C059676:octyl acetate
keywords: Bacteria; Biomarker.; GC-MS; SPME; VOC