title: The effects of desipramine, fluoxetine, or tianeptine on changes in bulbar BDNF levels induced by chronic social instability stress and inflammation.
abstract: BACKGROUND
  Stress is a major predisposing factor in the development of psychiatric disorders and potential source of augmented inflammatory processes in the brain. Increasing body of evidence shows an important role of alterations in the olfactory bulbs (OBs) function in stress-related disorders. The aim of the present study was to investigate the impact of antidepressants on the alterations of brain-derived neurotrophic factor (BDNF) induced by lipopolysaccharide (LPS) in female rats subjected to chronic social instability stress (CSIS).
  
  
  METHODS
  9 weeks old female rats were subjected to CSIS and injected ip once daily with desipramine (10mg/kg), fluoxetine (5mg/kg), or tianeptine (10mg/kg) for 4 weeks. On the last day of the experiment, rats being at the estrus phase of cycle were injected ip with LPS (1mg/kg) or saline.
  
  
  RESULTS
  The BDNF mRNA and protein levels were evaluated in the olfactory bulbs. and the BDNF protein levels were measured in plasma. A single LPS administration in the stressed rats resulted in significant decrease in the bulbar BDNF mRNA, but not in the protein level. Chronic administration of desipramine, fluoxetine, or tianeptine increased the BDNF mRNA expression and protein levels in the LPS-injected stressed rats. There was no effect of the studied antidepressants on the reduction of the plasma BDNF protein level induced by CSIS and LPS.
  
  
  CONCLUSIONS
  These results suggest that studied antidepressants were effective in inhibiting the impact of LPS on BDNF expression in the stressed rats what may be significant for beneficial action of this drugs.
authors.affiliation: Laboratory of Molecular Biology, Faculty of Physiotherapy, The Jerzy Kukuczka Academy of Physical Education, Katowice, Poland; Department of Pharmacology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland. Electronic address: m.nowacka@awf.katowice.pl.
authors.forename: Marta M
authors.initials: MM
authors.lastname: Nowacka-Chmielewska
authors.affiliation: Laboratory of Molecular Biology, Faculty of Physiotherapy, The Jerzy Kukuczka Academy of Physical Education, Katowice, Poland.
authors.forename: Daniela
authors.initials: D
authors.lastname: Kasprowska
authors.affiliation: Department of Medical Genetics, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Sosnowiec, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Paul-Samojedny
authors.affiliation: Department of Pharmacology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Bielecka-Wajdman
authors.affiliation: Department of Physiology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; Center for Experimental Medicine, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
authors.forename: Jarosław J
authors.initials: JJ
authors.lastname: Barski
authors.affiliation: Laboratory of Molecular Biology, Faculty of Physiotherapy, The Jerzy Kukuczka Academy of Physical Education, Katowice, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Małecki
authors.affiliation: Department of Pharmacology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Obuchowicz
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D003891:Desipramine / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000188:drug therapy* / Q000503:physiopathology; D013841:Thiazepines / Q000494:pharmacology*
chemical_list: D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013841:Thiazepines; D005473:Fluoxetine; C050504:tianeptine; D003891:Desipramine
keywords: Antidepressants; BDNF; Chronic social stress; LPS; Olfactory bulbs