title: A temperature-tolerant multiplex elements and genes screening system for genetically modified organisms based on dual priming oligonucleotide primers and capillary electrophoresis.
abstract: High throughput screening systems are the preferred solution to meet the urgent requirement of increasing number of genetically modified organisms (GMOs). In this study, we have successfully developed a multiplex GMO element screening system with dual priming oligonucleotide (DPO) primers. This system can detect the cauliflower mosaic virus 35S (CaMV 35S), terminator of nopaline synthase gene (NOS), figwort mosaic virus 35S (FMV 35S) promoter, neomycin phosphotransferaseII (NPTII), Bt Cry 1Ab, phosphinothricin acetyltransferase genes (bar) and Streptomyces viridochromogenes (pat) simultaneously, which covers more than 90% of all authorized GMO species worldwide. This system exhibits a high tolerance to annealing temperatures, high specificity and a limit of detection equal to conventional PCR. A total of 214 samples from markets, national entry-exit agencies, the Institute for Reference Materials and Measurement (IRMM) and the American Oil Chemists' Society (AOCS) were also tested for applicability. This screening system is therefore suitable for GMO screening.
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.affiliation: Shantou Entry-exit Inspection and Quarantine Bureau, Shantou 515041, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wei
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China; College of Plant Protection, China Agricultural University, Beijing 100193, China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Guangxi Entry-exit Inspection and Quarantine Bureau, Nanning 530028, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Du
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Department of Food Science and Engineering, College of Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Xiyang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China. Electronic address: wugang@caas.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China. Electronic address: zhusf@caiq.gov.cn.
authors.forename: Shuifang
authors.initials: S
authors.lastname: Zhu
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D030781:Organisms, Genetically Modified / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D017931:DNA Primers
keywords: Capillary electrophoresis; DPO primers; GMO screening; High-throughput; Wide coverage