title: Extremely low-frequency electromagnetic field promotes astrocytic differentiation of human bone marrow mesenchymal stem cells by modulating SIRT1 expression.
abstract: It has been shown that extremely low-frequency electromagnetic fields (ELFMF) affect regulation of cell fate and differentiation. Thus, the aim of this study was to investigate the role of ELFMFs in the enhancement of astrocytic differentiation. ELFMF exposure reduced the rate of proliferation and enhanced astrocytic differentiation. The ELFMF-treated cells showed increased levels of the astrocyte marker (GFAP), while those of the early neuronal marker (Nestin) and stemness marker (OCT3/4) were downregulated. The reactive oxygen species (ROS) level was observed to be significantly elevated after ELFMF exposure, which strengthens the modulatory role of SIRT1 and SIRT1 downstream molecules (TLE1, HES1, and MASH1) during astrocytic differentiation. After nicotinamide (5 mM) mediated inhibition of SIRT1, levels of TLE1, HES1, and MASH1 were examined; TLE1 was significantly upregulated and MASH1 was downregulated. These results suggest that ELFMFs induce astrocytic differentiation through activation of SIRT1 and SIRT1 downstream molecules.
authors.affiliation: a College of Life Sciences and Biotechnology , Korea University , Seoul , Korea.
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Jeong
authors.affiliation: a College of Life Sciences and Biotechnology , Korea University , Seoul , Korea.
authors.forename: Jun-Beom
authors.initials: JB
authors.lastname: Kim
authors.affiliation: a College of Life Sciences and Biotechnology , Korea University , Seoul , Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: a College of Life Sciences and Biotechnology , Korea University , Seoul , Korea.
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
mesh_terms: D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D056970:Co-Repressor Proteins; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics; D009536:Niacinamide / Q000494:pharmacology; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000072056:Transcription Factor HES-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D056970:Co-Repressor Proteins; D005904:Glial Fibrillary Acidic Protein; D064231:Nestin; D009536:Niacinamide; D050814:Octamer Transcription Factor-3; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; D056564:Sirtuin 1; D000072056:Transcription Factor HES-1; C065500:ASCL1 protein, human; C577484:NES protein, human; C494173:POU5F1 protein, human; C499043:TLE1 protein, human; C078493:HES1 protein, human; C447939:SIRT1 protein, human
keywords: SIRT1; TLE1; astrocytic differentiation; extremely low-frequency electromagnetic fields; hBM-MSCs