title: Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation.
abstract: Dynamic RNA modifications recently were considered to constitute another realm for biological regulation in the form of "RNA epigenetics". N6-methyladenosine (m6A), one of the most important modifications on RNA, plays a fundamental role in epigenetic regulation of the mammalian transcriptome. We recently established various liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS)-based methods for the sensitive and accurate determination of modified nucleosides in both DNA and RNA. Here, we describe a protocol to analyze m6A in RNA by LC-ESI-MS/MS. And this protocol also can be extended to the analysis of other modified nucleosides in both DNA and RNA.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China. bfyuan@whu.edu.cn.
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008745:Methylation; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D012313:RNA; C010223:N-methyladenosine; D000241:Adenosine
keywords: RNA Methylation, N 6-Methyladenosine; Liquid Chromatography-Mass Spectrometry