title: ChIP-seq for the Identification of Functional Elements in the Human Genome.
abstract: Functional elements in the genome express their function through physical association with particular proteins: transcription factors, components of the transcription machinery, specific histone modifications, and others. The genome-wide characterization of the protein-DNA interaction landscape of these proteins is thus a key approach toward the identification of candidate genomic regulatory regions. ChIP-seq (Chromatin Immunoprecipitation coupled with high-throughput sequencing) has emerged as the primary experimental methods for carrying out this task. Here, the ChIP-seq protocol is described together with some of the most important considerations for applying it in practice.
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, 47405, USA. marinovg@iu.edu.
authors.forename: Georgi K
authors.initials: GK
authors.lastname: Marinov
mesh_terms: D000818:Animals; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation / Q000379:methods; D015894:Genome, Human; D015698:Genomic Library; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006657:Histones / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D020218:Response Elements; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D006657:Histones; D014157:Transcription Factors
keywords: Chromatin immunoprecipitation; High-throughput sequencing; Histone modification; Regulatory element; Transcription factor