title: Quantitative analysis of lipids: a higher-throughput LC-MS/MS-based method and its comparison to ELISA.
abstract: OBJECTIVE
  Lipids such as prostaglandins, leukotrienes and thromboxanes are released as a result of an inflammatory episode in pain (central and peripheral).
  To measure these lipids as potential mechanistic biomarkers in neuropathic pain models, we developed a higher-throughput LC-MS/MS-based method with simultaneous detection of PGE2, PGD2, PGF2α, LTB4, TXB2 and 2-arachidonoyl glycerol in brain and spinal cord tissues. We also demonstrate that the LC-MS/MS method was more sensitive and specific in differentiating PGE2 levels in CNS tissues compared with ELISA.
  
  
  CONCLUSIONS
  The ability to modify the LC-MS/MS method to accommodate numerous other lipids in one analysis, demonstrates that the presented method offers a cost-effective and more sensitive alternative to ELISA method useful in drug discovery settings.
authors.affiliation: Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA; Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA.
authors.forename: Adarsh S
authors.initials: AS
authors.lastname: Gandhi
authors.affiliation: Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA; Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA.
authors.forename: David
authors.initials: D
authors.lastname: Budac
authors.affiliation: Neuroinflammation Disease Biology Unit, In Vitro Biology, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA; Neuroinflammation Disease Biology Unit, In Vitro Biology, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA.
authors.forename: Tanzilya
authors.initials: T
authors.lastname: Khayrullina
authors.affiliation: Neuroinflammation Disease Biology Unit, In Vitro Biology, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA; Neuroinflammation Disease Biology Unit, In Vitro Biology, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA.
authors.forename: Roland
authors.initials: R
authors.lastname: Staal
authors.affiliation: Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA; Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA.
authors.forename: Gamini
authors.initials: G
authors.lastname: Chandrasena
keywords: ELISA; LC–MS/MS; higher-throughput; lipids; neuropathic pain