title: Transcriptome Analysis and Identification of Major Detoxification Gene Families and Insecticide Targets in Grapholita Molesta (Busck) (Lepidoptera: Tortricidae).
abstract: The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), is an important pest of most stone and pome fruits and causes serious damage to the fruit industry worldwide. This insect pest has been primarily controlled through the application of insecticides; as a result, G. molesta has developed resistance to many different types of insecticides. To identify detoxification genes, we have, de novo, sequenced the transcriptome of G. molesta (Lepidoptera: Tortricidae) and yielded 58,970 unigenes of which 26,985 unigenes matched to known proteins. In total, 2,040 simple sequence repeats have been identified. The comprehensive transcriptome data set has permitted us to identify members of important gene families related to detoxification in G. molesta, including 77 unigenes of putative cytochrome P450s, 28 of glutathione S-transferases, 46 of Carboxylesterases, and 31 of insecticide targets. Orthologs of some of these unigenes have shown to play a pivotal role in insecticide resistance in other insect species and those unigenes likely have similar functions in G. molesta.
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China (guoyq1979@163.com; 1606673676@qq.com; butterflycoco@163.com; maruiyan2004@163.com).
authors.forename: Yanqiong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China (guoyq1979@163.com; 1606673676@qq.com; butterflycoco@163.com; maruiyan2004@163.com).
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chai
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China (guoyq1979@163.com; 1606673676@qq.com; butterflycoco@163.com; maruiyan2004@163.com).
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China (guoyq1979@163.com; 1606673676@qq.com; butterflycoco@163.com; maruiyan2004@163.com).
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: CSIRO Agriculture & Food, Private Bag 5, Wembley, WA 6913, Australia, and.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Gao
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China (guoyq1979@163.com; 1606673676@qq.com; butterflycoco@163.com; maruiyan2004@163.com).
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Ma
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D008658:Inactivation, Metabolic / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics; D007305:Insecticide Resistance; D007306:Insecticides; D008297:Male; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System
keywords: Grapholita molesta; detoxification gene; illumina sequencing; insecticide target; transcriptome