title: Effective Integration of Targeted Tumor Imaging and Therapy Using Functionalized InP QDs with VEGFR2 Monoclonal Antibody and miR-92a Inhibitor.
abstract: Rapid diagnosis and targeted drug treatment require agents that possess multiple functions. Nanomaterials that facilitate optical imaging and direct drug delivery have shown great promise for effective cancer treatment. In this study, we first modified near-infrared fluorescent indium phosphide quantum dots (InP QDs) with a vascular endothelial growth factor receptor 2 (VEGFR2) monoclonal antibody to afford targeted drug delivery function. Then, a miR-92a inhibitor, an antisense microRNA that enhances the expression of tumor suppressor p63, was attached to the VEGFR2-InP QDs via electrostatic interactions. The functionalized InP nanocomposite (IMAN) selectively targets tumor sites and allows for infrared imaging in vivo. We further explored the mechanism of this active targeting. The IMAN was endocytosed and delivered in the form of microvesicles via VEGFR2-CD63 signaling. Moreover, the IMAN induced apoptosis of human myelogenous leukemia cells through the p63 pathway in vitro and in vivo. These results indicate that the IMAN may provide a new and promising chemotherapy strategy against cancer cells, particularly by its active targeting function and utility in noninvasive three-dimensional tumor imaging.
authors.forename: Yi-Zhou
authors.identifier: http://orcid.org/0000-0001-9768-8144
authors.initials: YZ
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Maomao
authors.initials: M
authors.lastname: Pu
authors.forename: Gen
authors.identifier: http://orcid.org/0000-0001-9067-4786
authors.initials: G
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
authors.affiliation: Maternal and Child Health Institute, Nanjing Maternity and Child Health Care Hospital , Nanjing 210029, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000911:Antibodies, Monoclonal; D006801:Humans; D035683:MicroRNAs; D009369:Neoplasms; D045663:Quantum Dots; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2
chemical_list: D000911:Antibodies, Monoclonal; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: InP; VEGFR2; apoptosis; microvesicle; near-infrared fluorescent