title: Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis.
abstract: This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies fromLeishmania braziliensispatients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund's adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa fromL. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa ofL. braziliensis. The research on the similarity of the peptides' sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides' MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis.
authors.affiliation: Basic Pathology Department, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Juliana Seger
authors.initials: JS
authors.lastname: Link
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Silvana Maria
authors.initials: SM
authors.lastname: Alban
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Carlos Ricardo
authors.initials: CR
authors.lastname: Soccol
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Gilberto Vinicius Melo
authors.initials: GV
authors.lastname: Pereira
authors.affiliation: Basic Pathology Department, Federal University of Paraná, Curitiba, PR, Brazil; Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Vanete
authors.identifier: 0000-0001-9301-541X
authors.initials: V
authors.lastname: Thomaz Soccol
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification*; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007892:Leishmania braziliensis / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000276:immunology; D008647:Mesocricetus; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D019151:Peptide Library; D010455:Peptides