title: Controlling transmembrane protein concentration and orientation in supported lipid bilayers.
abstract: The trans-membrane protein - proteorhodopsin (pR) has been incorporated into supported lipid bilayers (SLB). In-plane electric fields have been used to manipulate the orientation and concentration of these proteins, within the SLB, through electrophoresis leading to a 25-fold increase concentration of pR.
authors.affiliation: School of Physics & Astronomy, University of Leeds, LS2 9JT, UK. s.d.evans@leeds.ac.uk.
authors.forename: P
authors.identifier: http://orcid.org/0000-0002-7875-7420
authors.initials: P
authors.lastname: Bao
authors.affiliation: Department of Molecular Biology & Biotechnology, University of Sheffield, S10 2TH, UK.
authors.forename: M L
authors.initials: ML
authors.lastname: Cartron
authors.affiliation: School of Biomedical Science, University of Leeds, LS2 9JT, UK.
authors.forename: K H
authors.initials: KH
authors.lastname: Sheikh
authors.affiliation: School of Physics & Astronomy, University of Leeds, LS2 9JT, UK. s.d.evans@leeds.ac.uk.
authors.forename: B R G
authors.initials: BRG
authors.lastname: Johnson
authors.affiliation: Department of Molecular Biology & Biotechnology, University of Sheffield, S10 2TH, UK.
authors.forename: C N
authors.initials: CN
authors.lastname: Hunter
authors.affiliation: School of Physics & Astronomy, University of Leeds, LS2 9JT, UK. s.d.evans@leeds.ac.uk.
authors.forename: S D
authors.initials: SD
authors.lastname: Evans
mesh_terms: D004586:Electrophoresis; D008051:Lipid Bilayers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D025604:Rhodopsins, Microbial / Q000032:analysis*
chemical_list: D008051:Lipid Bilayers; D025604:Rhodopsins, Microbial; C413548:proteorhodopsin