title: Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine in Tunisian Electric Steel Foundry Workers Exposed to Polycyclic Aromatic Hydrocarbons.
abstract: In this study, urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), as biomarker of oxidative DNA damage, was evaluated in Tunisian electric steel foundry workers and was associated with polycyclic aromatic hydrocarbon (PAH) exposure. Ninety-three healthy male workers were enrolled in the study; 8-oxodG was assessed by liquid chromatography-triple quadrupole mass spectrometry. Exposure to PAHs was evaluated by measuring 16 urinary PAHs (U-PAHs) and 8 monohydroxylated metabolites (OHPAHs). The median 8-oxodG level for all subjects was 3.20 µg l-1 (1.85 µg g-1 creatinine). No correlation between 8-oxodG and 1-hydroxypyrene or any other OHPAH was found. Significant linear correlations between 8-oxodG and some U-PAHs were found, particularly urinary acenaphthylene (r = 0.249), phenanthrene (r = 0.327), anthracene (r = 0.357), fluoranthene (r = 0.248), and pyrene (r = 0.244). Multiple regression analyses confirmed that urinary phenanthrene, anthracene, and naphthalene (the latter with a non-linear relationship) were predictors of 8-oxodG; job title, but not smoking, was a determinant of 8-oxodG; the variance explained by these models was up to 20%. The oxidative DNA damage assessed by urinary 8-oxodG was moderate and in the range of values reported in other occupational fields or in the general population. The results of this study indicate that the investigated biomarkers of PAH exposure were only minor contributors to urinary 8-oxodG.
authors.affiliation: Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, Monastir, Avanue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Mariem
authors.initials: M
authors.lastname: Hanchi
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milan and Epidemiology Unit, Fondazione IRCCS Ca' GrandaOspedale Maggiore Policlinico, Via S. Barnaba, 8-20122 Milan, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Campo
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milan and Epidemiology Unit, Fondazione IRCCS Ca' GrandaOspedale Maggiore Policlinico, Via S. Barnaba, 8-20122 Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Polledri
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milan and Epidemiology Unit, Fondazione IRCCS Ca' GrandaOspedale Maggiore Policlinico, Via S. Barnaba, 8-20122 Milan, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Olgiati
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milan and Epidemiology Unit, Fondazione IRCCS Ca' GrandaOspedale Maggiore Policlinico, Via S. Barnaba, 8-20122 Milan, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Consonni
authors.affiliation: Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, Monastir, Avanue Avicenne, 5000 Monastir, Tunisia.
authors.forename: Dalila
authors.initials: D
authors.lastname: Saidane-Mosbahi
authors.affiliation: Department of Clinical Sciences and Community Health, University of Milan and Epidemiology Unit, Fondazione IRCCS Ca' GrandaOspedale Maggiore Policlinico, Via S. Barnaba, 8-20122 Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Fustinoni
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000652:urine; D002273:Carcinogens / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D008669:Metallurgy; D016273:Occupational Exposure / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity* / Q000652:urine; D012044:Regression Analysis; D013232:Steel; D053719:Tandem Mass Spectrometry; D014416:Tunisia
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons; D013232:Steel; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-oxodG; electric steel foundry; oxidative DNA damage; polycyclic aromatic hydrocarbons