title: Lactobacillus paraplantarum11-1 Isolated from Rice Bran Pickles Activated Innate Immunity and Improved Survival in a Silkworm Bacterial Infection Model.
abstract: Lactic acid bacteria (LAB) have high immune system-stimulating activity and are considered beneficial for human health as probiotics in the gut. The innate immune system is highly conserved between mammals and insects. Microbe-associated molecular patterns (e.g., peptidoglycan and β-glucan) induce cytokine maturation, which, in silkworm larvae, leads to muscle contraction. The purpose of this study is to find a novel probiotic by using silkworm muscle contraction assay. In the present study, we isolated LAB derived from rice bran pickles. We selected highly active LAB to activate the innate immune system of the silkworm, which was assayed based on silkworm muscle contraction. Of various LAB,L. paraplantarum11-1 strongly stimulated innate immunity in the silkworm, leading to stronger silkworm contraction than a dairy-based LAB. Silkworms fed a diet containingL. paraplantarum11-1 exhibited tolerance against the pathogenicity ofPseudomonas aeruginosa. These findings suggest thatL. paraplantarum11-1 could be a useful probiotic for activating innate immunity.
authors.affiliation: Genome Pharmaceuticals Institute Co. Ltd.Tokyo, Japan; Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of TokyoTokyo, Japan; Department of Microbiology and Immunology, Teikyo University School of MedicineTokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishida
authors.affiliation: Genome Pharmaceuticals Institute Co. Ltd. Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishii
authors.affiliation: Teikyo University Institute of Medical Mycology Tokyo, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Nishiyama
authors.affiliation: Teikyo University Institute of Medical Mycology Tokyo, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Abe
authors.affiliation: Department of Microbiology and Immunology, Teikyo University School of Medicine Tokyo, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ono
authors.affiliation: Genome Pharmaceuticals Institute Co. Ltd.Tokyo, Japan; Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of TokyoTokyo, Japan; Teikyo University Institute of Medical MycologyTokyo, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sekimizu
keywords: Lactic acid bacteria; Lactobacillus sp.; Pseudomonas aeruginosa; infection; innate immunity; silkworm