title: Effects of Nano-Graphene Oxide on Testis, Epididymis and Fertility of Wistar Rats.
abstract: Nanoscale graphene oxide (NGO) has great potential in biomedicine by the virtue of its facile functionalization and tunable characteristics. Toxicity assessments have therefore become essential prior to its biomedical applications. This study examined the effects of NGO exposure on male reproductive function of adult Wistar rats. Rats were exposed intraperitoneally to three increasing doses, namely low-dose (0.4 mg/kg BW), mid-dose (2.0 mg/kg BW) and high-dose (10.0 mg/kg BW) of NGO. Repeated exposure of NGO for 15 and 30 days resulted in decreased epididymal sperm counts and elevated sperm abnormalities. Percentage of motile sperms was also significantly reduced due to the exposure. Activities of SOD, GPx and malondialdehyde concentration in the testes increased in a dose-specific manner. Results of the study demonstrated that high-dose of NGO resulted in considerable histological damage to testicular tissue which included atrophy of seminiferous tubules with reduction in germinal epithelium, germ cell loss and vacuolization. Low and mid-doses of NGO were not associated with sperm dysfunction or testis damage. Withdrawal of treatment for 30 days demonstrated significant recovery potential. Histology of epididymis and male fertility potential were not affected due to the NGO exposure. These findings are important for assessment of the risk involved in manufacturing, use and processing of the graphene oxide-based materials towards male reproductive function.
authors.affiliation: Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur, India.
authors.forename: Naresh Kumar
authors.initials: NK
authors.lastname: Nirmal
authors.affiliation: National Institute of Animal Welfare, MoEF & CC, Ballabhgarh, Haryana, India.
authors.forename: Kumud Kant
authors.initials: KK
authors.lastname: Awasthi
authors.affiliation: Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur, India.
authors.forename: Placheril Joseph
authors.initials: PJ
authors.lastname: John
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006108:Graphite / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D007248:Infertility, Male / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007274:Injections, Intraperitoneal; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D017208:Rats, Wistar; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D059550:Toxicity Tests, Subchronic
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D006108:Graphite; D010088:Oxidoreductases