title: MiRNA expression in the cartilage of patients with osteoarthritis.
abstract: Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA.
  
  
  
  Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a miRCURYTM [corrected] Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction.
  
  
  
  Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group).
  
  
  
  These findings may contribute to disease prevention and the development of therapeutic targets for OA.
authors.affiliation: Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.|Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kopańska
authors.affiliation: Clinical Orthopedics and Traumatology Department in Clinical Hospital 2, 35-301, Rzeszow, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Szala
authors.affiliation: Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.|Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czech
authors.affiliation: Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.|Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Gabło
authors.affiliation: Data Analysis Laboratory, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Rzeszow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gargasz
authors.affiliation: Clinical Orthopedics and Traumatology Department in Clinical Hospital 2, 35-301, Rzeszow, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Trzeciak
authors.affiliation: Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.|Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Warzywna 1A, 35-959, Rzeszow, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Zawlik
authors.affiliation: Clinical Orthopedics and Traumatology Department in Clinical Hospital 2, 35-301, Rzeszow, Poland. ssnela@poczta.onet.pl.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Snela
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism* / Q000601:surgery
chemical_list: D035683:MicroRNAs
keywords: Cartilage; Expression; Osteoarthritis; Pathogenesis; Patients; miRNA