title: Amperometric detection of glucose in fruit juices with polypyrrole-based biosensor with an integrated permselective layer for exclusion of interferences.
abstract: A novel polypyrrole (PPy)-based bilayer amperometric glucose biosensor integrated with a permselective layer has been developed for detection of glucose in the presence of interferences. It comprises of a PPy-GOx film grown, in the absence of electrolyte, as an inner layer, and a permselective PPy-Cl film as an outer layer. The PPy-GOx/PPy-Cl bilayer biosensor was effective in rejecting 98% of ascorbic acid and 100% of glycine, glutamic acid and uric acid. With an outer layer thickness of 6.6nm, the bilayer biosensor gave nearly identical glucose response to that of a single layer PPy-GOx biosensor. The biosensor also exhibited good reproducibility (1.9% rsd, n=10), high stability (more than 2months), wide linear range (0.5-24mM), low Km(8.4mM), high Imax(77.2μAcm-2), low detection limit (26.9μM) and good sensitivity (3.5μAcm-2mM-1). The bilayer biosensor was successfully employed for glucose determination in various fruit juices.
authors.affiliation: NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton 3800, Victoria, Australia.
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Ayenimo
authors.affiliation: NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton 3800, Victoria, Australia. Electronic address: Sam.Adeloju@monash.edu.
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Adeloju
mesh_terms: D001628:Beverages / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D005947:Glucose / Q000032:analysis / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D005947:Glucose
keywords: Amperometry; Biosensor; Fruit juices; Glucose; Glucose oxidase; Non-alcoholic beverages; Polypyrrole