title: SC3: consensus clustering of single-cell RNA-seq data.
abstract: Single-cell RNA-seq enables the quantitative characterization of cell types based on global transcriptome profiles. We present single-cell consensus clustering (SC3), a user-friendly tool for unsupervised clustering, which achieves high accuracy and robustness by combining multiple clustering solutions through a consensus approach (http://bioconductor.org/packages/SC3). We demonstrate that SC3 is capable of identifying subclones from the transcriptomes of neoplastic cells collected from patients.
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Vladimir Yu
authors.identifier: http://orcid.org/0000-0003-1893-2255
authors.initials: VY
authors.lastname: Kiselev
authors.affiliation: Cambridge Institute for Medical Research, Wellcome Trust/MRC Stem Cell Institute and Department of Haematology, University of Cambridge, Hills Road, Cambridge, UK.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kirschner
authors.affiliation: Department of Mathematics and naXys, University of Namur, Namur, Belgium.|ICTEAM, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Schaub
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Tallulah
authors.identifier: http://orcid.org/0000-0003-1120-2196
authors.initials: T
authors.lastname: Andrews
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Yiu
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|Epigenetics Programme, The Babraham Institute, Babraham, Cambridge, UK.
authors.forename: Tamir
authors.initials: T
authors.lastname: Chandra
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|EMBL-European Bioinformatics Institute, Hinxton, Cambridge, UK.
authors.forename: Kedar N
authors.initials: KN
authors.lastname: Natarajan
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|Epigenetics Programme, The Babraham Institute, Babraham, Cambridge, UK.|Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.
authors.forename: Wolf
authors.initials: W
authors.lastname: Reik
authors.affiliation: Department of Mathematics, Imperial College London, London, UK.
authors.forename: Mauricio
authors.identifier: http://orcid.org/0000-0002-1089-5675
authors.initials: M
authors.lastname: Barahona
authors.affiliation: Cambridge Institute for Medical Research, Wellcome Trust/MRC Stem Cell Institute and Department of Haematology, University of Cambridge, Hills Road, Cambridge, UK.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Green
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Hemberg
mesh_terms: D016000:Cluster Analysis; D066264:Datasets as Topic; D020869:Gene Expression Profiling / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D017423:Sequence Analysis, RNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D060388:Support Vector Machine