title: Post-Effort Changes in Activity of Traditional Diagnostic Enzymatic Markers in Football Players' Blood.
abstract: BACKGROUND
  Long-term and intensive physical effort causes metabolic and biochemical adaptations for both athletic and non-athletic objectives. Knowing the importance of aerobic training in football players, the aim of this study was to evaluate changes in the activity of: creatinine kinase (CK), creatine kinase MB (CKMB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), cholinesterase (ChE) and alkaline phosphatase (ALP) in response to a semi-long distance outdoor run under aerobic conditions among both female and male football players.
  
  
  METHODS
  Sixteen participants aged 21.9±2 years (women) and 18.4±0.5 years (men), all of them voluntarily recruited football players, took part in an outdoor run, the women covering a distance of 7.4±0.3 km while men covered a distance of 10.7±1.0 km. Plasma activities of the studied enzymes were determined using an appropriate diagnostic assay kit.
  
  
  RESULTS
  Our results indicate that total LDH activity could be a useful tool in evaluating physical fitness among athletes. We simultaneously established that ChE could not be a marker useful in assessing metabolic response to physical effort in athletes. Moreover, our results suggest that post-effort changes in ALP activity might be used to estimate early symptoms of certain vitamin deficiencies in an athlete's diet.
  
  
  CONCLUSIONS
  We confirmed that the assessment of activity of selected traditional diagnostic enzymatic markers provides information about muscle state after physical effort.
authors.affiliation: Faculty of Sport Education, Gdansk University of Physical Education and Sport, Gdansk, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Chamera
authors.affiliation: Institute of Sports, University School of Physical Education in Cracow, Cracow, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Spieszny
authors.affiliation: Institute of Sports, University School of Physical Education in Cracow, Cracow, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Klocek
authors.affiliation: Department of Biochemistry, University of Szczecin, Szczecin, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kostrzewa-Nowak
authors.affiliation: Department of Biochemistry, University of Szczecin, Szczecin, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Nowak
authors.affiliation: Faculty of Sport Education, Gdansk University of Physical Education and Sport, Gdansk, Poland.
authors.forename: Milena
authors.initials: M
authors.lastname: Lachowicz
authors.affiliation: Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Buryta
authors.affiliation: Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Ficek
authors.affiliation: Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Eider
authors.affiliation: Faculty of Sport Education, Gdansk University of Physical Education and Sport, Gdansk, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Moska
authors.affiliation: Faculty of Sport Education, Gdansk University of Physical Education and Sport, Gdansk, Poland; Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Cięszczyk
keywords: aerobic training; athletes; enzymatic markers