title: Comparison of batch cultivation strategies for cost-effective biomass production of Micractinium inermum NLP-F014 using a blended wastewater medium.
abstract: Two competitive strategies, fed-batch and sequencing-batch cultivation, were compared in cost-effective biomass production of a high lipid microalgae, Micractinium inermum NLP-F014 using a blended wastewater medium. For fed-batch cultivations, additional nutrient was supplemented at day 2 (FB1) or consecutively added at day 2 and 4 (FB2). Through inoculum size test, 1.0g-DCWL-1was selected for the sequencing-batch cultivation (SB) where about 65% of culture was replaced with fresh medium every 2days. Both fed-batch cultivations showed the maximum biomass productivity of 0.95g-DCWL-1d-1, while average biomass productivity in SB was slightly higher as 0.96±0.08g-DCWL-1d-1. Furthermore, remained concentrations of organics (426mg-CODL-1), total nitrogen (15.4mg-NL-1) and phosphorus (0.6mg-PL-1) in SB were much lower than those of fed-batch conditions. The results suggested that SB could be a promising strategy to cultivate M. inermum NLP-F014 with the blended wastewater medium.
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea.
authors.forename: Seonghwan
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea.
authors.forename: Jeongmi
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea.
authors.forename: Younghyun
authors.initials: Y
authors.lastname: Park
authors.affiliation: Information Analysis Center, Korea Institute of Science and Technology Information, 1217, Jungang-daero Road, Yeon-ji-gu, Busan 46241, Republic of Korea.
authors.forename: Suyoung
authors.initials: S
authors.lastname: Son
authors.affiliation: Department of Microbiology, School of Natural Science, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea.
authors.forename: Sunja
authors.initials: S
authors.lastname: Cho
authors.affiliation: Institute of Environmental Technology and Industry, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea.
authors.forename: Changwon
authors.initials: C
authors.lastname: Kim
authors.affiliation: Department of Civil and Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea. Electronic address: leeth55@pusan.ac.kr.
authors.forename: Taeho
authors.initials: T
authors.lastname: Lee
mesh_terms: D061249:Batch Cell Culture Techniques / Q000191:economics; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors / Q000191:economics; D000460:Chlorophyta / Q000254:growth & development*; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry; D058086:Microalgae / Q000254:growth & development*; D009584:Nitrogen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D062065:Wastewater / Q000737:chemistry
chemical_list: D056804:Biofuels; D003470:Culture Media; D009584:Nitrogen; D010758:Phosphorus; D062065:Wastewater
keywords: Biomass productivity; Blended wastewater medium; Fed-batch cultivation; Microalgae; Sequencing-batch cultivation