title: Online Hydrogen-Deuterium Exchange Traveling Wave Ion Mobility Mass Spectrometry (HDX-IM-MS): a Systematic Evaluation.
abstract: Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is an important tool for measuring and monitoring protein structure. A bottom-up approach to HDX-MS provides peptide level deuterium uptake values and a more refined localization of deuterium incorporation compared with global HDX-MS measurements. The degree of localization provided by HDX-MS is proportional to the number of peptides that can be identified and monitored across an exchange experiment. Ion mobility spectrometry (IMS) has been shown to improve MS-based peptide analysis of biological samples through increased separation capacity. The integration of IMS within HDX-MS workflows has been commercialized but presently its adoption has not been widespread. The potential benefits of IMS, therefore, have not yet been fully explored. We herein describe a comprehensive evaluation of traveling wave ion mobility integrated within an online-HDX-MS system and present the first reported example of UDMSEacquisition for HDX analysis. Instrument settings required for optimal peptide identifications are described and the effects of detector saturation due to peak compression are discussed. A model system is utilized to confirm the comparability of HDX-IM-MS and HDX-MS uptake values prior to an evaluation of the benefits of IMS at increasing sample complexity. Interestingly, MS and IM-MS acquisitions were found to identify distinct populations of peptides that were unique to the respective methods, a property that can be utilized to increase the spatial resolution of HDX-MS experiments by >60%. Graphical Abstract ᅟ.
authors.affiliation: LGC, Queens Road, Teddington, London, TW11 0LY, UK. adam.cryar@lgcgroup.com.
authors.forename: Adam
authors.initials: A
authors.lastname: Cryar
authors.affiliation: LGC, Queens Road, Teddington, London, TW11 0LY, UK.
authors.forename: Kate
authors.initials: K
authors.lastname: Groves
authors.affiliation: LGC, Queens Road, Teddington, London, TW11 0LY, UK.
authors.forename: Milena
authors.initials: M
authors.lastname: Quaglia
mesh_terms: D041961:Deuterium Exchange Measurement / Q000379:methods*; D000075663:Ion Mobility Spectrometry / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009862:Online Systems; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000032:analysis; D014168:Transferrin / Q000032:analysis / Q000737:chemistry
chemical_list: D011506:Proteins; D014168:Transferrin; D012710:Serum Albumin, Bovine
keywords: Data-independent acquisition; Hydrogen-deuterium exchange mass spectrometry; Ion mobility; MSE; Protein structure