title: Polymorphisms of Platelet Glycoprotein Receptors and Cell Adhesion Molecules in Fetuses with Fetal Growth Restriction and Their Mothers As Detected with Pyrosequencing.
abstract: Vascular thrombotic tendency may lead to fetal growth restriction (FGR). Altered platelet function and genetic heterogeneity may play a role in this procedure. We investigated whether maternal or fetal genotypic frequencies of genes polymorphisms for certain platelet receptor and cell adhesion molecules are altered in FGR.
  
  
  
  We compared the maternal and fetal genotypic frequencies of single nucleotide polymorphisms (SNPs) in four genes coding for platelet receptors and cell adhesion molecules [integrin alpha subunit 2 (ITGA2)C807T, integrin subunit beta 3(ITGB3) T1565C, platelet cell adhesion protein 1 (PECAM1) CTG-GTG and selectin P(SELP)A/C]. A total of 32 fetuses with fetal growth restriction and their mothers were matched with 18 normal controls. Using maternal venous blood and umbilical cord blood samples, nucleotide sequences were determined from pyrograms. Genotypic frequencies were calculated and analyzed using appropriate tests and logistic regression.
  
  
  
  There was no statistical difference in the proportion of heterozygotes or homozygotes for any of the genotypic frequencies between FGR and control groups in mothers or fetuses.
  
  
  
  Our study demonstrated no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with FGR.
authors.affiliation: Second Department of Obstetrics and Gynecology, Medical School, Aretaieio Hospital, National and Kapodistrian University of Athens, Athens, Greece maria.simou@ymail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Simou
authors.affiliation: Laboratory of Therapeutic Individualization, Second Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, Medical School, Aretaieio Hospital, Athens, Greece.
authors.forename: Evaggelia
authors.initials: E
authors.lastname: Kouskouni
authors.affiliation: Second Department of Obstetrics and Gynecology, Medical School, Aretaieio Hospital, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Vitoratos
authors.affiliation: Laboratory of Therapeutic Individualization, Second Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, Medical School, Aretaieio Hospital, Athens, Greece.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Economou
authors.affiliation: Second Department of Obstetrics and Gynecology, Medical School, Aretaieio Hospital, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Creatsas
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics*; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics*; D005333:Fetus / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics; D039661:Integrin beta3 / Q000235:genetics; D016015:Logistic Models; D009035:Mothers; D019007:P-Selectin / Q000235:genetics; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D015815:Cell Adhesion Molecules; C056564:ITGA2B protein, human; C061248:ITGB3 protein, human; D039421:Integrin alpha2; D039661:Integrin beta3; D019007:P-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010980:Platelet Membrane Glycoproteins; C546937:SELP protein, human
keywords: FGR; SNPs; platelet glycoproteins; pyrosequencing