title: In Vivo Model of Small Intestine.
abstract: The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.
authors.affiliation: Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA.|Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA.
authors.forename: Maxime M
authors.initials: MM
authors.lastname: Mahe
authors.affiliation: Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Brown
authors.affiliation: Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA.
authors.forename: Holly M
authors.initials: HM
authors.lastname: Poling
authors.affiliation: Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA. michael.helmrath@cchmc.org.|Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA. michael.helmrath@cchmc.org.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Helmrath
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D006801:Humans; D007421:Intestine, Small / Q000166:cytology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008954:Models, Biological; D009940:Organoids / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000166:cytology
keywords: Bioengineered small intestine; Human intestinal organoids; In vivo model; Kidney capsule; Mesentery; Pluripotent stem cells; Transplantation