title: Proteomic Characteristics of Blood Serum in Rats with Different Behavioral Parameters after Acute Stress.
abstract: We studied proteome profile of blood serum of Wistar rats with different behavioral activity immediately and in 1 and 3 days after acute stress on the model of 12-h immobilization during the nighttime. Comparative analysis of 2D-electrophoretograms revealed differences in the expression of serum proteins in non-stressed (control) and stressed (experimental) rats. We found 22 protein spots that characterized the proteomic features of blood serum in rats with different prognostic resistance to stress. Mass-spectrometry of isolated spots identified 6 functional proteins. Persistent proteome changes in the blood of animals at different stages after acute stress were determined. The specificity of proteomic characteristics of blood serum was shown in behaviorally passive and active rats during the post-stress period. These data extend the concept on specific protein markers for the formation of a negative emotional state and adaptive-and-compensatory processes in mammals with different sensitivity to stressogenic factors.
authors.affiliation: Research Institute of Nutrition, Moscow, Russia. n.kirbaeva@gmail.com.
authors.forename: N V
authors.initials: NV
authors.lastname: Kirbaeva
authors.affiliation: Research Institute of Nutrition, Moscow, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Sharanova
authors.affiliation: Research Institute of Nutrition, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Vasil'ev
authors.affiliation: P. K. Anokhin Research Institute of Normal Physiology, Moscow, Russia.|A. E. Evdokimov Moscow State University of Medicine and Density, Moscow, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Pertsov
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1 / Q000097:blood; D007147:Immunoglobulin Light Chains / Q000097:blood; D034904:Lamin Type A / Q000097:blood; D008297:Male; D054465:Peroxiredoxin VI / Q000097:blood; D020543:Proteome / Q000378:metabolism; D017208:Rats, Wistar; D064067:Rho Guanine Nucleotide Exchange Factors / Q000097:blood; D013315:Stress, Psychological / Q000097:blood*
chemical_list: D001798:Blood Proteins; D007147:Immunoglobulin Light Chains; D034904:Lamin Type A; D020543:Proteome; D064067:Rho Guanine Nucleotide Exchange Factors; C090764:prelamin A; D054465:Peroxiredoxin VI; C515984:Prdx6 protein, rat; D019392:Cytochrome P-450 CYP2E1
keywords: behaviorally passive and active rats; blood; immobilization stress; proteomics