title: Polysaccharides extraction from Opuntia stricta and their protective effect against HepG2 cell death and hypolipidaemic effects on hyperlipidaemia rats induced by high-fat diet.
abstract: The aim of this study was to analyse cytoprotective effect of polysaccharides compound from Opuntia stricta (O. stricta) cladode (POS) in vitro including its radical scavenging activities and protective effects against hypercholesterolaemia. Our results showed that glucose was the dominant monosaccharides (30.35%). Arabinose, pyranose, fructose, galactose, glucose, sorbitol, S-inositol, M-inositol, trehalose and saccharose found in this species. O. stricta polysaccharides did not cause any cytotoxic effect on HepG2 cells within the range of concentrations tested (0-400 μgml-1). Pre-treatment of HepG2 cells with POS (100 μgml-1) significantly (p < .05) protected against cytotoxicity induced by DPPH and ABTS radicals. The POS showed strong antioxidant potential in vitro. The results indicated also that POS significantly prevented hypercholesterolaemia-induced elevation of serum biomarkers and induced increase in serum lipid profile. Moreover, the hypercholesterolaemia characterised by elevated lipid peroxidation (MDA) and reduced antioxidant enzyme defences (SOD, CAT and GPx) was restored by POS treatment.
authors.affiliation: a Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Bahira
authors.initials: B
authors.lastname: Harrabi
authors.affiliation: b Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Athmouni
authors.affiliation: c Laboratory of Histology and Embryology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Latifa
authors.initials: L
authors.lastname: Hamdaoui
authors.affiliation: a Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Lobna
authors.initials: L
authors.lastname: Ben Mahmoud
authors.affiliation: a Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hakim
authors.affiliation: b Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.affiliation: a Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Zeghal
authors.affiliation: a Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Hanéne
authors.initials: H
authors.lastname: Ghozzi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D056945:Hep G2 Cells; D006801:Humans; D006949:Hyperlipidemias / Q000209:etiology / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D029747:Opuntia / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides
keywords: HepG2 cell line; Opuntia stricta polysaccharides; histology; hypercholesterolaemia; stress biomarkers