title: Dalbergioidin (DAL) protects MC3T3-E1 osteoblastic cells against H2O2-induced cell damage through activation of the PI3K/AKT/SMAD1 pathway.
abstract: Reactive oxygen species (ROS) is a pivotal pathogenic factor in the development of osteoporosis. Dalbergioidin (DAL) can be isolated from Uraria crinite, an edible herb used as a natural food for childhood skeletal dysplasia. Recent research has implicated DAL as having an antiosteoporosis effect, although the mechanism of this is unclear. We used an effective oxidative stress model, induced by hydrogen peroxide (H2O2) in osteoblastic MC3T3-E1 cells, to investigate the protective effects of DAL in osteoporosis and the underlying molecular mechanisms. The results indicated that treatment with DAL maintained redox balance, reduced MC3T3-E1 cell apoptosis, improved alkaline phosphatase activity, and elevated the osteogenic-related protein expression of Runx2, Osterix, and BMP2 against oxidative damage induced by H2O2. The potential molecular mechanism involved in the protective effect of DAL against H2O2-induced cell death in MC3T3-E1 cells may lie in the activation of the PI3K/AKT/SMAD1 cell signal pathway. Taken together, the results indicated that the potential protective effects of DAL against osteoporosis were linked to a reduction in oxidative damage, suggesting that DAL may be useful in bone metabolism diseases, particularly osteoporosis.
authors.affiliation: Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, China.|Center Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Jin
authors.affiliation: Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, China.
authors.forename: Jia-Ling
authors.initials: JL
authors.lastname: Li
authors.affiliation: Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, China.
authors.forename: Jin-Dong
authors.initials: JD
authors.lastname: Chen
authors.affiliation: Key Laboratory of SATCM for Empirical Formulae Evaluation and Achievements Transformation, The No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Chang-Liang
authors.initials: CL
authors.lastname: Xu
authors.affiliation: Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, China. lihuang76@nju.edu.cn.
authors.forename: Huang
authors.initials: H
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010024:Osteoporosis / Q000188:drug therapy; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects*; D051898:Smad1 Protein / Q000502:physiology*
chemical_list: D002867:Chromones; D051898:Smad1 Protein; C497401:Smad1 protein, mouse; C531366:dalbergioidin; D006861:Hydrogen Peroxide; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D000469:Alkaline Phosphatase
keywords: Apoptosis; Dalbergioidin (DAL); Osteoporosis; Oxidative stress; PI3K/AKT/SMAD1 pathway