title: MAPK/ERK signalling is required for zebrafish cardiac regeneration.
abstract: OBJECTIVE
  To better understand the molecular mechanisms of regeneration and explore the potential signalling pathways as therapeutic targets for heart attacks.
  
  
  RESULTS
  After treatment with the MEK inhibitor AZD6244 upon cardiac injury, the core members in MAPK/ERK signalling-mek and erk-demonstrate elevated expression, and these proteins are deposited at the injury site in zebrafish. pERK is also induced in non-cardiomyocytes near the injury site. Furthermore, the induced expression of a dominant-negative form of MEK1 inhibits zebrafish cardiac regeneration, characterized by increased cardiac fibrosis (a hallmark of regenerative failure), reduced or delayed production of regenerative myocardium, and migration of FLI1+endothelial cells, without direct inhibition of cardiomyocyte proliferation.
  
  
  CONCLUSIONS
  Appropriate activation of MAPK/ERK signalling is essential for zebrafish cardiac regeneration.
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, Fudan University School of Life Sciences, E203 Life Science Building, 2005 Songhu Road, Shanghai, 200438, China.
authors.forename: Peiyun
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, Fudan University School of Life Sciences, E203 Life Science Building, 2005 Songhu Road, Shanghai, 200438, China. zhongtpfudan@163.com.|Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, 37232, USA. zhongtpfudan@163.com.
authors.forename: Tao P
authors.initials: TP
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000008:administration & dosage; D006321:Heart / Q000502:physiology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000008:administration & dosage; D012038:Regeneration; D015398:Signal Transduction; D015027:Zebrafish / Q000502:physiology*
chemical_list: C517975:AZD 6244; D001562:Benzimidazoles; D047428:Protein Kinase Inhibitors; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Cardiac regeneration; MAPK/ERK signalling; MEK; Zebrafish; pERK