title: Identification and expression analysis of terminal deoxynucleotidyl transferase in humphead snapper Lutjanus sanguineus.
abstract: A tdt gene was identified successfully from humphead snapper Lutjanus sanguineus, which contained 1710 bp encoding a protein of 463 amino acids. Results of quantitative real-time polymerase chain reaction (qRT-PCR) indicated that tdt mainly expressed in thymus and head kidney and the transcripts of tdt in these tissues were up-regulated significantly at 36 and 48 h after Vibrio harveyi infection. Meanwhile Tdt-producing cells were found in thymus and head kidney.
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: J F
authors.initials: JF
authors.lastname: Tang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Wang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: J C
authors.initials: JC
authors.lastname: Jian
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wu
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004253:DNA Nucleotidylexotransferase / Q000235:genetics / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D010473:Perciformes / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D029941:Fish Proteins; D004253:DNA Nucleotidylexotransferase
keywords: TdT; Vibrio harveyi; immunohistochemistry; tdt gene