title: Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification.
abstract: On-site monitoring the plantation of genetically modified (GM) crops is of critical importance in agriculture industry throughout the world. In this paper, a simple, visual and instrument-free method for instant on-site detection of GTS 40-3-2 soybean has been developed. It is based on body-heat recombinase polymerase amplification (RPA) and followed with naked-eye detection via fluorescent DNA dye. Combining with extremely simplified sample preparation, the whole detection process can be accomplished within 10 min and the fluorescent results can be photographed by an accompanied smart phone. Results demonstrated a 100% detection rate for screening of practical GTS 40-3-2 soybean samples by 20 volunteers under different ambient temperatures. This method is not only suitable for on-site detection of GM crops but also demonstrates great potential to be applied in other fields.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Biological Science and Engineering, Fuzhou University , Fuzhou, 350108, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Qian
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Jian
authors.identifier: 0000-0003-4518-7027
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Ying
mesh_terms: D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010781:Photography; D030821:Plants, Genetically Modified / Q000235:genetics; D045522:Recombinases / Q000378:metabolism*; D030262:Soybean Proteins / Q000235:genetics; D013025:Glycine max / Q000235:genetics*; D013696:Temperature
chemical_list: D018744:DNA, Plant; D005456:Fluorescent Dyes; D045522:Recombinases; D030262:Soybean Proteins