title: Different Cold-Signaling Pathways Function in the Responses to Rapid and Gradual Decreases in Temperature.
abstract: In plants, cold temperatures trigger stress responses and long-term responses that result in cold tolerance. InArabidopsis thaliana, three dehydration-responsive element (DRE) binding protein 1/C-repeat binding factors (DREB1/CBFs) act as master switches in cold-responsive gene expression. Induction ofDREB1genes triggers the cold stress-inducible transcriptional cascade, followed by the induction of numerous genes that function in the cold stress response and cold tolerance. Many regulatory factors involved inDREB1induction have been identified, but how these factors orchestrate the cold stress-specific expression ofDREB1shas not yet been clarified. Here, we revealed that plants recognize cold stress as two different signals, rapid and gradual temperature decreases, and induce expression of theDREB1genes. CALMODULIN BINDING TRANSCRIPTION ACTIVATOR3 (CAMTA3) and CAMTA5 respond to a rapid decrease in temperature and induce the expression ofDREB1s, but these proteins do not respond to a gradual decrease in temperature. Moreover, they function during the day and night, in contrast to some key circadian components, including CIRCADIAN CLOCK ASSOCIATED1 and LATE ELONGATED HYPOCOTYL, which regulate cold-responsiveDREB1expression as transcriptional activators only during the day. Thus, plants efficiently control the acquisition of freezing tolerance using two different signaling pathways in response to a gradual temperature decrease during seasonal changes and a sudden temperature drop during the night.
authors.affiliation: Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kidokoro
authors.affiliation: Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Koshi
authors.initials: K
authors.lastname: Yoneda
authors.affiliation: Gene Discovery Research Group, RIKEN Centre for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Takasaki
authors.affiliation: Gene Discovery Research Group, RIKEN Centre for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Takahashi
authors.affiliation: Gene Discovery Research Group, RIKEN Centre for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.affiliation: Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan akys@mail.ecc.u-tokyo.ac.jp.
authors.forename: Kazuko
authors.identifier: http://orcid.org/0000-0002-0249-8258
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D058639:Cold-Shock Response / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C572689:AT2G22300 protein, Arabidopsis; D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; D014157:Transcription Factors