title: Fluorescent multi-responsive cross-linked P(N-isopropylacrylamide)-based nanocomposites for cisplatin delivery.
abstract: Magnetic, pH and temperature-sensitive, poly(N-isopropylacrylamide) (PNIPAM)-based nanocomposites with fluorescent properties were synthesized by free radical copolymerization-cross linking of NIPAM, N,N-dimethylaminoethyl methacrylate (DMAEMA) and 4-acrylamidofluorescein (AFA). The model anti-cancer drug, cisplatin (CDDP), was loaded into the resulted nanogel. For the production of CDDP-loaded nanocomposite, Fe3O4magnetic nanoparticles (MNPs) and CDDP were loaded into the nanogel. Field-emission scanning electron microscopy (FE-SEM) indicated that the size of nanogel and CDDP-loaded nanocomposite were about 90 and 160 nm, respectively. The encapsulation efficiency of CCDP was found up to 65%. The loaded CCDP showed sustained thermal and pH-responsive drug release. A high level of drug release was observed under the conditions of low pH and high temperature. The lower critical solution temperature (LCST) of synthesized nanogel was about 40 °C. CDDP-loaded nanocomposite showed a volume phase transition from 282 to 128 nm at its LCST. Accordingly, in this study, the synthesized nanocomposite can be employed as a stimuli-responsive anti-cancer drug delivery system and the pH and temperature of solution have the potential to monitor the drug release.
authors.affiliation: a Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Shakoori
authors.affiliation: a Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ghanbari
authors.affiliation: b Faculty of Pharmacy, Research Center for Pharmaceutical Nanotechnology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Omidi
authors.affiliation: c Women's Reproductive Health Research Centre , Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Anatomical Sciences, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Pashaiasl
authors.affiliation: e Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|f Universal Scientific Education and Research Network (USERN) , Tabriz , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: a Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Jomeh Farsangi
authors.affiliation: a Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.|g Department of Toxicology and Pharmacology, School of Pharmacy, Pharmaceutical Sciences Branch , Islamic Azad University (IAUPS) , Tehran , Iran.|h Department of Pharmacology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.|i Experimental Medicine Research Center , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Rezayat
authors.affiliation: e Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|j Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Davaran
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D002945:Cisplatin / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D000080385:Nanogels; D053758:Nanoparticles / Q000737:chemistry*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: C000622531:4-acrylamidofluorescein; D000178:Acrylamides; D000180:Acrylic Resins; D000970:Antineoplastic Agents; D005452:Fluoresceins; D008689:Methacrylates; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; C049840:2-(dimethylamino)ethyl methacrylate; D002945:Cisplatin
keywords: Cisplatin; controlled drug delivery; fluorescent; multi-responsive magnetic nanocomposite