title: Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species.
abstract: Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus speciesCrocus oliverissp.Oliveri, using the MS medium containing 5% (w/v) sucrose supplemented with (4 mg/L NAA + 4 mg/L TDZ) and (2 mg/L IAA + 2 mg/L TDZ + 2 mg/L BAP). When the embryogenic calli were transferred into the four nutrient media containing (2 mg/L IAA + 2 mg/L TDZ) and 100 mg/L ABA, these further developed into cotyledonary embryos. Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was obtained inC. oliverissp.Oliveri. During subculture using the half strength media, cotyledonary embryos gradually developed into plantlets.
authors.affiliation: Abant Izzet Baysal University, Department of Biology, Bolu 14280, Turkey.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Verma
authors.affiliation: Gyeongsang National University, School of Mechanical Engineering, Jinju, 660701, South Korea, South Korea.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Das
authors.affiliation: Abant Izzet Baysal University, Department of Biology, Bolu 14280, Turkey.
authors.forename: Gunce Sahin
authors.initials: GS
authors.lastname: Cingoz
authors.affiliation: Abant Izzet Baysal University, Department of Biology, Bolu 14280, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Uslu
authors.affiliation: Abant Izzet Baysal University, Department of Biology, Bolu 14280, Turkey.
authors.forename: Ekrem
authors.initials: E
authors.lastname: Gurel
keywords: ABA, abscisic acid; BAP, 6-benzylaminopurine; CHE, Chee and Pool medium; Crocus species; GB5, Gamborg’s B-5 medium; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid; LS, Linsmaier and Skoog medium; MS, Murashige and Skoog medium; NAA, α-naphthalene acetic acid; Organogeneis; Plant regeneration; Somatic embryogenesis; TDZ, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea