title: Dietary enzymatically treated Artemisia annua L. supplementation alleviates liver oxidative injury of broilers reared under high ambient temperature.
abstract: Heat stress induced by high ambient temperature is a major concern in commercial broiler production. To evaluate the effects of dietary enzymatically treated Artemisia annua L. (EA) supplementation on growth performance and liver oxidative injury of broilers reared under heat stress, a total of 320 22-day-old male broilers were randomly allotted into five groups with eight replicates of eight birds each. Broilers in the control group were housed at 22 ± 1 °C and fed the basal diet. Broilers in the HS, HS-EA1, HS-EA2, and HS-EA3 groups were fed basal diet supplemented with 0, 0.75, 1.00, and 1.25 g/kg EA, respectively, and reared under cyclic high temperature (34 ± 1 °C for 8 h/day and 22 ± 1 °C for 16 h/day). Broilers fed EA diets had higher final body weight, average daily body weight gain, and average daily feed intake, as well as liver concentration of reduced glutathione, activities of antioxidant enzymes, abilities to inhibit hydroxyl radical and superoxide radical (HS-EA2 and HS-EA3), and lower liver concentrations of reactive oxygen metabolites, malondialdehyde, and protein carbonyl (HS-EA1, HS-EA2, and HS-EA3) than HS group (P < 0.05). EA treatment downregulated the mRNA levels of heat shock proteins 70 and 90, upregulated the mRNA levels of nuclear factor erythroid 2-related factor 2 (HS-EA1, HS-EA2, and HS-EA3) and heme oxygenase 1 (HS-EA2 and HS-EA3) in liver of heat-treated broilers (P < 0.05). In conclusion, EA alleviated heat stress-induced growth depression and liver oxidative injury in broilers, possibly through improving the antioxidant capacity and regulating the pertinent mRNA expression. The appropriate inclusion level of EA in broiler diet is 1.00-1.25 g/kg.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wan
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Jingfei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Jintian
authors.initials: J
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Kaiwen
authors.initials: K
authors.lastname: Bai
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. tianwangnjau@163.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
mesh_terms: D000821:Animal Feed; D000818:Animals; D031172:Artemisia annua / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D019587:Dietary Supplements; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D018882:Heat Stress Disorders / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control* / Q000662:veterinary; D051547:Heme Oxygenase-1 / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics; D018384:Oxidative Stress; D011201:Poultry Diseases / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Artemisia annua L.; Broiler; Gene expression; Growth performance; Heat stress; Oxidative injury