title: Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification.
abstract: Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes overexpress a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis. A significant increase in the EpCAM-positive exosome level in these patients was detected, compared to healthy controls. Furthermore, we quantified circulating HER2-positive exosomes in 19 cases of breast cancer patients for molecular classification. We demonstrated that the exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining. The microfluidic chip might provide a new platform to assist breast cancer diagnosis and molecular classification.
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Shimeng
authors.initials: S
authors.lastname: Fang
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Hongzhu
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Urology, the Second Affiliated Hospital, Dalian Medical University, Dalian, China.
authors.forename: Xiancheng
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Kong
authors.affiliation: Department of Nuclear Medicine, the First Affiliated Hospital, Dalian Medical University, Dalian, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.|State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian, China.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Niu
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Tingjiao
authors.identifier: http://orcid.org/0000-0001-5850-789X
authors.initials: T
authors.lastname: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism*; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D009360:Neoplastic Cells, Circulating; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D018719:Erb-b2 Receptor Tyrosine Kinases; C499379:EPCAM protein, human; C508053:ERBB2 protein, human