title: MicroRNA-17-92 Regulates the Transcription Factor E2F3b during Myogenesis In Vitro and In Vivo.
abstract: Myogenic differentiation, which occurs during muscle development, is a highly ordered process that can be regulated by E2F transcription factors. Available data show that E2F3b, but not E2F3a, is upregulated and required for myogenic differentiation. However, the regulation of E2F3b expression in myogenic differentiation is not well understood. To investigate whether E2Fb expression is controlled by miRNAs, we used bioinformatics to combine the database of microRNAs downregulated during myogenesis and those predicted to target E2F3. This identified miR-17 and miR-20a as miRNAs potentially involved in E2F3 regulation. We found that miR-17-92 controls the expression of E2F3b in C2C12 cells during myogenic differentiation. Moreover, we confirmed that miR-20a regulates the expression of E2F3b proteins in vivo using a muscle regeneration model.
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. zhizhi0719s@163.com.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. liunian27@hotmail.com.
authors.forename: Nian
authors.initials: N
authors.lastname: Liu
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. lanluochen@hotmail.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518000, China. kangkang@szu.edu.cn.
authors.forename: Kang
authors.initials: K
authors.lastname: Kang
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. ylili@szu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China. niruiyang@pku.edu.cn.
authors.forename: Ruiyang
authors.initials: R
authors.lastname: Ni
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. huiling.qiu.chavany@gmail.com.|Biomedical Engineering, College of Health and Environmental Engineering, Shenzhen Technology University, Shenzhen 51000, China. huiling.qiu.chavany@gmail.com.
authors.forename: Huiling
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China. dmgou@szu.edu.cn.
authors.forename: Deming
authors.initials: D
authors.lastname: Gou
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D054715:Cardiotoxins; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D050699:E2F3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D008856:Microscopy, Fluorescence; D024510:Muscle Development / Q000235:genetics* / Q000502:physiology; D009135:Muscular Diseases / Q000139:chemically induced / Q000503:physiopathology; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D012038:Regeneration / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D054715:Cardiotoxins; D050699:E2F3 Transcription Factor; C490840:E2f3 protein, mouse; C575895:MIRN17-92 microRNA, mouse; D035683:MicroRNAs
keywords: E2F3b; miR-20a; muscle