title: The importance of arginine codons AGA and AGG for the expression inE. coliof triosephosphate isomerase from seven different species.
abstract: Rare arginine codons AGA and AGG affect the heterologous expression of proteins inEschericha coli. The tRNAs necessary for protein synthesis are scarce inE. colistrain BL21(DE3) pLysS and plentiful in strain BL21(DE3) CodonPlus -RIL. We evaluated in both bacterial strains the effect of these rare codons on the expression of triosephosphate isomerases from 7 different species, whose sequences had different dispositions of rare arginine codons. The ratio of expressed protein (CP/Bl21) correlated with the number of rare codons. Our study shows that the number, position and particularities of the combination of rare Arg codons in the natural non-optimized sequences of the triosephosphate isomerases influence the synthesis of heterologous proteins inE. coliand could have implications in the selection of better sequences for engineering enzymes for novel or manipulated metabolic pathways or for the expression levels of non enzymatic proteins..
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Av. Universidad 3000, Coyoacán, 04510 México DF, Mexico.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Aguirre-López
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Av. Universidad 3000, Coyoacán, 04510 México DF, Mexico.
authors.forename: Nallely
authors.initials: N
authors.lastname: Cabrera
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Av. Universidad 3000, Coyoacán, 04510 México DF, Mexico.
authors.forename: Marietta Tuena
authors.initials: MT
authors.lastname: de Gómez-Puyou
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Av. Universidad 3000, Coyoacán, 04510 México DF, Mexico.
authors.forename: Ruy
authors.initials: R
authors.lastname: Perez-Montfort
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Av. Universidad 3000, Coyoacán, 04510 México DF, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Gómez-Puyou
keywords: Escherichia coli; IPTG, isopropyl β-d-thiogalactopyranoside; Protein expression; Rare codons; TIM, triosephosphate isomerase; Triosephosphate isomerase; mRNA, messenger ribonucleic acid; tRNA, transfer ribonucleic acid