title: Saponins fromSanguisorba officinalisImprove Hematopoiesis by Promoting Survival through FAK and Erk1/2 Activation and Modulating Cytokine Production in Bone Marrow.
abstract: Radix Sanguisorbae, the root ofSanguisorba officinalisL. is used as traditional Chinese medicine. In recent decades, it has been reported to be clinically effective against myelosuppression induced by chemotherapy and/ or radiotherapy. However, the underlining mechanism has not been well studied. In this work, we evaluated the hematopoietic effect of total saponins fromS. officinalisL. on myelosuppressive mice induced by cyclophosphamide and by60Co-γ-irradiation and confirmed the therapeutic effect. Then, we found total saponins and their characteristic constituents Ziyuglycoside I and Ziyuglycoside II can inhibit apoptosis of TF-1 cells caused by cytokine deprivation, and promote survival of mouse bone marrow nuclear cells through focal adhesion kinase (FAK) and extracellular signal-regulated kinase 1/2 (Erk1/2) activationin vitro. In addition, they can down-regulate macrophage inflammatory protein 2 (MIP-2), platelet factor 4 (PF4) and P-selectin secretion, which are reported to be suppressive to hematopoiesis, bothin vitroandin vivo. These results suggest that promotion of survival through FAK and Erk1/2 activation and inhibition of suppressive cytokines in the bone marrow is likely to be the pharmacological mechanism underlying the hematopoietic effect of saponins fromS. officinalisL.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences (CAS)Sichuan, China; Graduate School, University of Chinese Academy of Sciences (CAS)Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences (CAS)Sichuan, China; Di Ao Pharmaceutical GroupSichuan, China.
authors.forename: Bogang
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences (CAS) Sichuan, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Ji
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences (CAS) Sichuan, China.
authors.forename: Zhongliu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences (CAS) Sichuan, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chen
keywords: Sanguisorba officinalis L.; Ziyuglycoside I; Ziyuglycoside II; apoptosis; cytokine; hematopoiesis; myelosuppression; saponin