title: Heparanase overexpression down-regulates syndecan-1 expression in a gallbladder carcinoma cell line.
abstract: Objective To discuss the relevance of heparanase and syndecan-1 and regulation of the heparanase-syndecan1 axis in the invasiveness of gallbladder carcinoma cells. Methods 1. Generation of a gallbladder cancer cell line overexpressing a heparanase (GBD-SD) transgene. 2. Western blot analysis of syndecan-1 levels of GBD-SD and control gallbladder carcinoma (GBC-SD) cells. 3. RT-PCR analysis of syndecan-1 mRNA levels of GBD-SD and GBC-SD. 4. Evaluation of invasion and migration of GBD-SD and GBC-SD cells. Results 1. Heparanase expression in GBD-SD cells was significantly increased. 2. The syndecan-1 mRNA level of GBD-SD cells was significantly lower compared with that of GBC-SD cells. 3. The syndecan-1 DNA copy number in GBD-SD cells was significantly lower compared with that of GBC-SD. 4. The invasiveness and migration of GBD-SD cells were significantly higher compared with GBC-SD cells. Conclusions 1. The expression of heparanase negatively correlated with that of syndecan-1 in a gallbladder carcinoma cell line. 2. The expression of heparanase and syndecan-1 in gallbladder carcinomas negatively correlated, similar to other tumours. 3. The heparanase/syndecan1 axis in gallbladder carcinoma plays an important role in the invasion and metastasis, thus providing a new therapeutic target. 4. Further research is required to identify the detailed mechanisms.
authors.affiliation: 1 Zhuhai People's Hospital, Zhuhai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jin
authors.affiliation: 1 Zhuhai People's Hospital, Zhuhai, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zhou
authors.affiliation: 2 The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
authors.forename: Song
authors.initials: S
authors.lastname: Yang
authors.affiliation: 1 Zhuhai People's Hospital, Zhuhai, China.
authors.forename: Hai-Ming
authors.initials: HM
authors.lastname: Cao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D056915:DNA Copy Number Variations; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005704:Gallbladder / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D053668:Syndecan-1 / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D019076:Transgenes; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D012333:RNA, Messenger; D053668:Syndecan-1; D000099175:Heparanase
keywords: Gallbladder carcinoma; RT-PCR; western blot; heparanase; metastasis; syndecan-1