title: Origin of α-mannosidase activity in CSF.
abstract: The α-mannosidase activity in human frontal gyrus, cerebrospinal fluid and plasma has been analyzed by DEAE-cellulose chromatography to investigate the origin of the α-mannosidase activity in cerebrospinal fluid (CSF). The profile of α-mannosidase isoenzymes obtained in CSF was similar to that in the frontal gyrus but different from that in human plasma. In particular the two characteristic peaks of lysosomal α-mannosidase, A and B, which have a pH-optimum of 4.5 and are found in human tissues, were present in both the frontal gyrus and CSF. In contrast the majority of α-mannosidase activity in human plasma was due to the so called intermediate form, which has a pH-optimum of 5.5. The results suggest that the intermediate form of α-mannosidase in plasma does not cross the blood-brain barrier and that the α-mannosidase activity present in the cerebrospinal fluid is of lysosomal type and of brain origin. Thus the α-mannosidase activity in cerebrospinal fluid might mirror the brain pathological changes linked to neurodegenerative disorders such as Parkinson's disease.
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Tasegian
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Paciotti
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
authors.forename: Maria Rachele
authors.initials: MR
authors.lastname: Ceccarini
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Codini
authors.affiliation: Department of Anatomy and Neurosciences, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Tim
authors.initials: T
authors.lastname: Moors
authors.affiliation: Department of Medicine, Section of Neurology, University of Perugia, Perugia, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Chiasserini
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Albi
authors.affiliation: Biochemistry Research Group, UCL Institute of Child Health, University College London, UK.
authors.forename: Bryan
authors.initials: B
authors.lastname: Winchester
authors.affiliation: Department of Anatomy and Neurosciences, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Wilma D J
authors.initials: WDJ
authors.lastname: van de Berg
authors.affiliation: Department of Medicine, Section of Neurology, University of Perugia, Perugia, Italy.
authors.forename: Lucilla
authors.initials: L
authors.lastname: Parnetti
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy. Electronic address: tommaso.beccari@unipg.it.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Beccari
mesh_terms: D005625:Frontal Lobe / Q000378:metabolism; D006801:Humans; D009928:Organ Specificity; D043323:alpha-Mannosidase / Q000097:blood / Q000134:cerebrospinal fluid*
chemical_list: D043323:alpha-Mannosidase
keywords: Brain; CSF; Lysosomes; MAN2B1; Parkinson; α-Mannosidase