title: Exploring the Antimicrobial and Antitumor Potentials ofStreptomycessp. AGM12-1 Isolated from Egyptian Soil.
abstract: The occurrence of extensive antibiotics resistant bacteria increased the demands for mining out new sources of antimicrobial agents. Actinomycetes, especiallyStreptomycessp. have grasped considerable attention worldwide due to production of many useful bioactive metabolites. In the present study, a total of 52 actinomycetes were isolated from agricultural soil samples in Beni-Suef, Egypt. All isolates were characterized based on colony morphology, mycelium coloration, and pigment diffusion. They were screened for their capabilities to show antimicrobial activities against different indicator microorganisms, and only 20 isolates have shown significant antimicrobial activities against at least one of the tested indicator microorganisms. The isolate AGM12-1 was active against all tested microorganisms and showed a marked antitumor activity with IC503.3 and 1.1 μg/ml against HCT-116 and HepG-2 cell lines respectively. It was genotypically characterized asStreptomycessp. with the presence of PKS Π biosynthetic gene cluster. Mannitol, ammonium sulfate, pH 7, 2% inoculum size and incubation for 11 days at 30°C were the optimum conditions that used to maximize the production and hence allowed purification of one active antimicrobial compound to homogeneity using high performance liquid chromatography with a molecular mass of m/z 488.05. Nuclear magnetic resonance structural elucidation showed that this compound was a diketopiperazine derivative.
authors.affiliation: Botany and Microbiology Department, Faculty of Science, Beni-Suef University Beni-Suef, Egypt.
authors.forename: Maged S
authors.initials: MS
authors.lastname: Ahmad
authors.affiliation: Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University Beni-Suef, Egypt.
authors.forename: Ahmed O
authors.initials: AO
authors.lastname: El-Gendy
authors.affiliation: Zoology Department, Faculty of Science, Beni-Suef University Beni-Suef, Egypt.
authors.forename: Rasha R
authors.initials: RR
authors.lastname: Ahmed
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University Beni-Suef, Egypt.
authors.forename: Hossam M
authors.initials: HM
authors.lastname: Hassan
authors.affiliation: Health Research Department, The National Center for Radiation Research and Technology, Atomic Energy Authority Beni-Suef, Egypt.
authors.forename: Hussein M
authors.initials: HM
authors.lastname: El-Kabbany
authors.affiliation: Botany and Microbiology Department, Faculty of Science, Beni-Suef University Beni-Suef, Egypt.
authors.forename: Ahmed G
authors.initials: AG
authors.lastname: Merdash
keywords: Streptomyces sp.; Streptomyces vinaceusdrappus; actinomycetes; antimicrobial; antitumor; diketopiperazine