title: Identification of Novel MAGE-G1-Interacting Partners in Retinoic Acid-Induced P19 Neuronal Differentiation Using SILAC-Based Proteomics.
abstract: MAGE-G1 is a protein plays role in the early process of neurogenesis. However, the fundamental roles MAGE-G1 played in neurogenesis have not yet been completely understood. Finding the partners MAGE-G1 interacting with will surely contribute to the function study of MAGE-G1. In this study, using Stable Isotope Labeling by Amino acids in Cell culture-immunoprecipitation quantitative proteomics, we screened the interacting proteins of MAGE-G1 during retinoic acid -induced neuronal differentiation of P19 cells and firstly found that FSCN1 and VIME were potential novel MAGE-G1-interacting proteins. Then, the interaction between overexpressed MAGE-G1 and FSCN1 or VIME was validated by GST-pull down assay in bacteria and by co-immunoprecipitation assay in COS7 cells. Endogenous co-immunoprecipitation assay further confirmed that MAGE-G1 interacted with FSCN1 or VIME in P19 cells after a 6-day retinoic acid-induced neuronal differentiation. Those results provide a functional linkage between MAGE-G1 and FSCN1 or VIME and may facilitate a better understanding of the fundamental aspects of MAGE-G1 during neurogenesis.
authors.affiliation: State Key Laboratory of Proteomics, Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Proteomics, Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Yujian
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Proteomics, Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Shide
authors.initials: S
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Proteomics, Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Proteomics, Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, 100850, China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D015972:Gene Expression Regulation, Neoplastic; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007553:Isotope Labeling; D058950:Melanoma-Specific Antigens / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics / Q000379:methods; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014212:Tretinoin / Q000494:pharmacology*; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism
chemical_list: D005982:Glutathione Transferase; D058950:Melanoma-Specific Antigens; D008840:Microfilament Proteins; D018035:Receptors, Odorant; D011993:Recombinant Fusion Proteins; D014212:Tretinoin; D014746:Vimentin; C000627185:Vim protein, mouse; C544141:Olfr73 protein, mouse