title: NMR-based metabolomics Reveals Alterations of Electro-acupuncture Stimulations on Chronic Atrophic Gastritis Rats.
abstract: Chronic atrophic gastritis (CAG) is a common gastrointestinal disease which has been considered as precancerous lesions of gastric carcinoma. Previously, electro-acupuncture stimulation has been shown to be effective in ameliorating symptoms of CAG. However the underlying mechanism of this beneficial treatment is yet to be established. In the present study, an integrated histopathological examination along with molecular biological assay, as well as1H NMR analysis of multiple biological samples (urine, serum, stomach, cortex and medulla) were employed to systematically assess the pathology of CAG and therapeutic effect of electro-acupuncture stimulation at Sibai (ST 2), Liangmen (ST 21), and Zusanli (ST 36) acupoints located in the stomach meridian using a rat model of CAG. The current results showed that CAG caused comprehensive metabolic alterations including the TCA cycle, glycolysis, membrane metabolism and catabolism, gut microbiota-related metabolism. On the other hand, electro-acupuncture treatment was found able to normalize a number of CAG-induced metabolomics changes by alleviating membrane catabolism, restoring function of neurotransmitter in brain and partially reverse the CAG-induced perturbation in gut microbiota metabolism. These findings provided new insights into the biochemistry of CAG and mechanism of the therapeutic effect of electro-acupuncture stimulations.
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Xujuan
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Bioprocess &Polymer Engineering, Innovative Centre in Agritechnology, University Teknologi Malaysia, Johor 81310, Malaysia.
authors.forename: Kian-Kai
authors.initials: KK
authors.lastname: Cheng
authors.affiliation: College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Shen
authors.affiliation: College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Shen
authors.affiliation: College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Qida
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Electronic Science, and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China.
authors.forename: Zongbao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015671:Electroacupuncture; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D005757:Gastritis, Atrophic / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats