title: Two-Dimensional Correlation Spectroscopy for Multimodal Analysis of FT-IR, Raman, and MALDI-TOF MS Hyperspectral Images with Hamster Brain Tissue.
abstract: Hyperspectral imaging (HSI) techniques are useful for obtaining very detailed structural and compositional information from biomedical, pharmaceutical, or clinical samples, among others. The informative value of these methods can be further increased through the application of different HSI techniques and joint analysis of the data. However, interpretation and understanding of multimodal HSI have been impeded by difficulties in registration of the different HSI data sets and by the lack of integrative analysis concepts. Here, we introduce two-dimensional correlation spectroscopy (2DCOS) as a novel technique for jointly analyzing HSI data which allows one to obtain deeper insights into the chemistry of complex samples by decrypting auto- and heterospectral correlations that may exist between features of the different HSI data. The general workflow of 2DCOS analysis is demonstrated by HSI examples acquired from cryo-sections of hamster brain tissue using Fourier-transform infrared (FT-IR) microspectroscopy, confocal Raman microspectroscopy (CRM), and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Multimodal hyperspectral image analysis by 2DCOS opens up new opportunities for spectral band assignments and thus the interpretation of structure-spectra and composition-spectra relationships. We foresee wide application potential for describing complex samples in various fields ranging from biomedicine to industrial applications.
authors.affiliation: ZBS6 "Proteomics and Spectroscopy", Robert Koch-Institute , Nordufer 20, D-13353 Berlin, Germany.
authors.forename: Peter
authors.identifier: 0000-0001-6193-3144
authors.initials: P
authors.lastname: Lasch
authors.affiliation: Department of Materials Science and Engineering, University of Delaware , Newark, Delaware 19716, United States.
authors.forename: Isao
authors.initials: I
authors.lastname: Noda
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D000078331:Correlation of Data; D005260:Female; D008647:Mesocricetus; D064847:Multimodal Imaging / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*