title: Derivation of Human Embryonic Stem Cells.
abstract: Embryonic stem cells (ESCs) represent a mainstay for pluripotent stem cell research and development (R&D) and provide tangible opportunities for clinical translation including cell therapies and drug discovery. Moreover, in spite of the discovery of induced pluripotent stem cells (iPSCs), ESCs are an essential reference point, against which other pluripotent cells are compared. Hence, there is an ongoing need to derive and bank quality-controlled research-grade and clinical-grade ESC lines using established and standardized methods. Here, we provide a concise, step-by-step protocol for the derivation of ESCs from human embryos. While largely based on previously reported method for clinical-grade human ESC (hESC) line derivation, the protocol is suitable for routine application, although adaptable for clinical-compliance.
authors.affiliation: ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.|Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia. jcrook@uow.edu.au.|Department of Surgery, St Vincent's Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia. jcrook@uow.edu.au.
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Crook
authors.affiliation: Centre for Blood Cell Therapies, Peter MacCallum Cancer Centre, East Melbourne, Australia.
authors.forename: Lucy
authors.initials: L
authors.lastname: Kravets
authors.affiliation: Genea Biomedx, Level 2, 321 Kent Street, Sydney, NSW, 2000, Australia.
authors.forename: Teija
authors.initials: T
authors.lastname: Peura
authors.affiliation: Department of Medicine, Division of Endocrinology and Stem Cell Institute, McGuire Translational Research Facility, University of Minnesota, 2001 6th Street SE, Mail Code 2873, Minneapolis, MN, 55455, USA.
authors.forename: Meri T
authors.initials: MT
authors.lastname: Firpo
mesh_terms: D018070:Biological Specimen Banks / Q000592:standards; D002460:Cell Line; D046149:Embryo Culture Techniques / Q000592:standards*; D004622:Embryo, Mammalian / Q000166:cytology; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans
keywords: Blastocyst; Derivation; Human embryonic stem cells; Inner cell mass