title: Long Noncoding RNA PVT1 Promotes EMT and Cell Proliferation and Migration Through Downregulating p21 in Pancreatic Cancer Cells.
abstract: OBJECTIVE
  Long noncoding RNA-plasmacytoma variant translocation 1 is identified to be highly expressed and exhibits oncogenic activity in a variety of human malignancies, including pancreatic cancer. However, little is known about the overall biological role and mechanism of plasmacytoma variant translocation 1 in pancreatic cancer so far. In this study, we investigated the effect of plasmacytoma variant translocation 1 on pancreatic cancer cell proliferation and migration as well as epithelial-mesenchymal transition.
  
  
  METHODS
  Pancreatic cancer tissue specimens and cell line were used in this study, with normal tissue and cell line acting as control.
  
  
  RESULTS
  It showed that plasmacytoma variant translocation 1 expression was significantly upregulated in pancreatic cancer tissues or cell line compared to normal groups. Plasmacytoma variant translocation 1 downregulation significantly inhibited zinc finger E-box-binding protein 1/Snail expression but promoted p21 expression, and it also inhibited the cell proliferation and migration. Additionally, p21 downregulation enhanced, and p21 overexpression repressed, zinc finger E-box-binding protein 1/Snail expression and cells proliferation in PANC-1 cells. However, p21 downregulation reversed the effect of plasmacytoma variant translocation 1 downregulation on zinc finger E-box-binding protein 1/Snail expression and cell proliferation and migration.
  
  
  CONCLUSIONS
  Plasmacytoma variant translocation 1 promoted epithelial-mesenchymal transition and cell proliferation and migration through downregulating p21 in pancreatic cancer cells.
authors.affiliation: Department of Hepatatobiliary Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
authors.forename: Bao-Qiang
authors.initials: BQ
authors.lastname: Wu
authors.affiliation: Department of Hepatatobiliary Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Hepatatobiliary Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Hepatatobiliary Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
authors.forename: Dong-Lin
authors.initials: DL
authors.lastname: Sun
authors.affiliation: Department of Hepatatobiliary Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu Province, China.
authors.forename: Xiao-Zhou
authors.initials: XZ
authors.lastname: He
keywords: EMT; PVT1; lncRNA; p21; pancreatic cancer