title: Expression of claudin-11, -23 in different gastric tissues and its relationship with the risk and prognosis of gastric cancer.
abstract: Claudins play an important role in regulating the permeability of epithelial and endothelial cells and in the maintenance of cell polarity. We aimed to investigate expression of claudin-11, -23 in different gastric tissues and its relationship with clinicopathologic parameters and prognosis of gastric cancer. We compared their expression levels in the paired cancerous tissues versus those in the adjacent noncancerous tissues by real-time PCR, western blotting and immunohistochemistry. The results showed that the expression of claudin-11, -23 was greatly increased in paracancerous gastric tissue compared with cancerous tissue. We also compared their expression levels of tissues from gastric cancer, superficial gastritis, and atrophic gastritis by immunohistochemistry. The results indicated that the expression of claudin-11 and 23 was significantly higher in superficial gastritis than that in atrophic gastritis and gastric cancer. The expression of claudin-23 was significantly lower in atrophic gastritis than that in gastric cancer, but no obviously difference was observed for claudin-11. As for analysis of clinicopathologic parameters of gastric cancer, logistic multiple regression indicated that claudin-11 was significantly associated with sex, smoking, alcohol, H. pylori infection and Borrmann classification while claudin-23 was significantly associated with vessel cancer embolus. Cox multivariate survival analysis indicated that gastric cancer patients with negative claudin-23 expression had significantly longer overall survival. In conclusion, the expression of claudin-11, -23 was remarkably downregulated in gastric cancer. Abnormal expression of these proteins was significantly correlated with some clinicopathologic parameters. In particular, claudin-23 positive expression was associated with poor prognostic outcomes of gastric cancer patients and may therefore serve as an independent prognosticator of patient survival.
authors.affiliation: Digestive department, The First Affiliated Hospital of China Medical University, Liaoning, Shenyang, Liaoning, China.
authors.forename: Youzhu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, Liaoning, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Jing
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, Liaoning, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, Liaoning, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Digestive department, The First Affiliated Hospital of China Medical University, Liaoning, Shenyang, Liaoning, China.
authors.forename: Moye
authors.initials: M
authors.lastname: Chen
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, Liaoning, China.
authors.forename: Zeyang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Digestive department, The First Affiliated Hospital of China Medical University, Liaoning, Shenyang, Liaoning, China.
authors.forename: Mingjun
authors.identifier: http://orcid.org/0000-0001-8748-4658
authors.initials: M
authors.lastname: Sun
authors.affiliation: Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, Liaoning, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D015153:Blotting, Western; D057167:Claudins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000150:complications / Q000235:genetics / Q000378:metabolism; D005757:Gastritis, Atrophic / Q000150:complications / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000150:complications / Q000235:genetics* / Q000378:metabolism
chemical_list: C120453:CLDN11 protein, human; C481943:CLDN23 protein, human; D057167:Claudins