title: Facile preparation of a fluorescent probe to detect the cellular ability of nucleotide excision repair.
abstract: We previously developed a method to detect the cellular ability of nucleotide excision repair, which functions to remove UV-induced lesions in DNA, using a plasmid-type fluorescent probe. A drawback to the popular use of this method was that the oligonucleotide containing the (6-4) photoproduct, which was used as a primer in the plasmid preparation, must be synthesized chemically. In this study, we prepared the probe using a post-synthetically UV-irradiated oligonucleotide as the primer. Transfection of cells demonstrated that this probe detected the repair ability of the cells in the same manner as the original probe.
authors.affiliation: Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Hana
authors.initials: H
authors.lastname: Tawarahara
authors.affiliation: Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Kuraoka
authors.affiliation: Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan. Electronic address: iwai@chem.es.osaka-u.ac.jp.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
mesh_terms: D004249:DNA Damage / Q000235:genetics* / Q000528:radiation effects; D004260:DNA Repair / Q000235:genetics*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D014162:Transfection; D014466:Ultraviolet Rays; D051760:Xeroderma Pigmentosum Group A Protein / Q000235:genetics*
chemical_list: D005456:Fluorescent Dyes; D051760:Xeroderma Pigmentosum Group A Protein
keywords: DNA damage; DNA repair; Fluorescent probe; Plasmid; Ultraviolet light