title: Actinomycete Metabolome Induction/Suppression with N-Acetylglucosamine.
abstract: The metabolite profiles of three sponge-derived actinomycetes, namely, Micromonospora sp. RV43, Rhodococcus sp. RV157, and Actinokineospora sp. EG49 were investigated after elicitation with N-acetyl-d-glucosamine.1H NMR fingerprint methodology was utilized to study the differences in the metabolic profiles of the bacterial extracts before and after elicitation. Our study found that the addition of N-acetyl-d-glucosamine modified the secondary metabolite profiles of the three investigated actinomycete isolates. N-Acetyl-d-glucosamine induced the production of 3-formylindole (11) and guaymasol (12) in Micromonospora sp. RV43, the siderophore bacillibactin 16, and surfactin antibiotic 17 in Rhodococcus sp. RV157 and increased the production of minor metabolites actinosporins E-H (21-24) in Actinokineospora sp. EG49. These results highlight the use of NMR fingerprinting to detect changes in metabolism following addition of N-acetyl-d-glucosamine. N-Acetyl-d-glucosamine was shown to have multiple effects including suppression of metabolites, induction of new metabolites, and increased production of minor compounds.
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University , Brisbane, QLD 4111 Australia.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Dashti
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University , Brisbane, QLD 4111 Australia.
authors.forename: Tanja
authors.identifier: 0000-0002-6537-3997
authors.initials: T
authors.lastname: Grkovic
authors.affiliation: Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg , Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany.
authors.forename: Usama Ramadan
authors.initials: UR
authors.lastname: Abdelmohsen
authors.affiliation: Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg , Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Hentschel
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University , Brisbane, QLD 4111 Australia.
authors.forename: Ronald J
authors.identifier: 0000-0002-4022-2623
authors.initials: RJ
authors.lastname: Quinn
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D039903:Actinobacteria / Q000737:chemistry / Q000235:genetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D007211:Indoles; D055442:Metabolome; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011161:Porifera / Q000382:microbiology*
chemical_list: C528934:3-formylindole; D000900:Anti-Bacterial Agents; D007211:Indoles; D000117:Acetylglucosamine