title: Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution.
abstract: OBJECTIVE
  To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution.
  
  
  METHODS
  MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images.
  
  
  RESULTS
  Medial thickness, RV/S+LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation.
  
  
  CONCLUSIONS
  The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH. J. Med. Invest. 64: 43-49, February, 2017.
authors.affiliation: Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University.
authors.forename: Mikio
authors.initials: M
authors.lastname: Sugano
authors.forename: Homare
authors.initials: H
authors.lastname: Yoshida
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Kurobe
authors.forename: Hiroki
authors.initials: H
authors.lastname: Arase
authors.forename: Hajime
authors.initials: H
authors.lastname: Kinoshita
authors.forename: Takashi
authors.initials: T
authors.lastname: Kitaichi
authors.forename: Noriko
authors.initials: N
authors.lastname: Sugasawa
authors.forename: Soichiro
authors.initials: S
authors.lastname: Nakayama
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Maeda
authors.forename: Minoru
authors.initials: M
authors.lastname: Irahara
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D036101:Cord Blood Stem Cell Transplantation; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology; D064593:Heterografts; D006801:Humans; D006976:Hypertension, Pulmonary / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016686:Monocrotaline / Q000633:toxicity; D011651:Pulmonary Artery / Q000473:pathology
chemical_list: D016686:Monocrotaline