title: Hepatoprotective activity of Rhus oxyacantha root cortex extract against DDT-induced liver injury in rats.
abstract: The present investigation aimed to study the antioxidant activity and hepatoprotective effects of ethyl acetate extract of R. oxyacantha root cortex (RE) against DDT-induced liver injury in male rats. The RE exhibited high total phenolic, flavonoid and condensed tannins contents. The antioxidant activity in vitro systems showed a significant potent free radical scavenging activity of the extract. The HPLC finger print of R. oxyacantha active extract showed the presence of five phenolic compounds with higher amounts of catechol and gallic acid. The in vivo results showed that a single intraperitoneal administration of DDT enhanced levels of hepatic markers (ALT, AST and LDH) in serum of experimental animals. It also increased the oxidative stress markers resulting in increased levels of the lipid peroxidation with a significant induction of SOD and GPx, metallothioneins (MTs) and a concomitant decrease of non protein thiols (NPSH) in liver. However, pretreatment of rats with RE at a dose of 150 and 300mg/kg body weight significantly lowered serum transaminases and LDH in treated rats. A significant reduction in hepatic thiobarbituric reactive substances and a decrease in antioxidant enzymes activities and hepatic MTs levels by treatment with plant extract against DDT, were observed. These biochemical changes were consistent with histopathological observations, suggesting marked hepatoprotective effect of RE with the two doses used. These results strongly suggest that treatment with ethyl acetate extract normalizes various biochemical parameters and protects the liver against DDT-induced oxidative damage in rats and thus help in evaluation of traditional claim on this plant.
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia; Laboratory of Aromatic and Medicinal Plants, Biotechnology Center, Borj-Cédria Technopark, BP 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Hanène
authors.initials: H
authors.lastname: Ben Miled
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia. Electronic address: zaineb.ben.barka@gmail.com.
authors.forename: Zaineb Ben
authors.initials: ZB
authors.lastname: Barka
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Dorsaf
authors.initials: D
authors.lastname: Hallègue
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Lahbib
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ladjimi
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Mounira
authors.initials: M
authors.lastname: Tlili
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Khémais Ben
authors.initials: KB
authors.lastname: Rhouma
authors.affiliation: Laboratory of Aromatic and Medicinal Plants, Biotechnology Center, Borj-Cédria Technopark, BP 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Riadh
authors.initials: R
authors.lastname: Ksouri
authors.affiliation: Laboratory of Integrated Physiology, Science Faculty of Bizerte, Carthage University, 7021, Jarzouna, Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: Tebourbi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D003634:DDT / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008111:Liver Function Tests / Q000379:methods; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D029045:Rhus / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D020011:Protective Agents; D003634:DDT; D001219:Aspartate Aminotransferases
keywords: Ethyl acetate extract; Hepatotoxicity; Oxidative stress; Polyphenols; Rhus oxyacantha; Transaminases