title: Effect of pH on stability, conformation, and chaperone activity of erythroid & non-erythroid spectrin.
abstract: Spectrin, a major component of the eukaryotic membrane skeleton, has been shown to have chaperone like activity. Here we investigate the pH induced changes in the structure and stability of erythroid and brain spectrin by spectroscopic methods. We also correlate these changes with modulations of chaperone potential at different pH. We have followed the pH induced structural changes by circular dichroism spectroscopy and intrinsic tryptophan fluorescence. It is seen that lowering the pH from 9 has little effect on structure of the proteins till about pH6. At pH4, there is significant change of the secondary structure of the proteins, along with a 5nm hypsochromic shift of the emission maxima. Below pH4 the proteins undergo acid denaturation. Probing exposed hydrophobic patches on the proteins using protein-bound 8-anilinonaphthalene-1-sulfonate fluorescence demonstrates that there is higher solvent accessibility of hydrophobic surfaces in both forms of spectrin at around pH4. Dynamic light scattering and 90° light scattering studies show that the both forms of spectrin forms oligomers at pH~4. Chemical unfolding data shows that these oligomers are less stable than the tetrameric form. Aggregation studies with BSA show that at pH4, both spectrins exhibit better chaperone activity. This enhancement of chaperone like activity appears to result from an increase in regions of solvent-exposed hydrophobicity and oligomeric state of the spectrins which in turn are induced by moderately acid pH. This may have in-vivo implications in cells facing stress conditions where cytoplasmic pH is lowered.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, HBNI, Kolkata, India.
authors.forename: Dipayan
authors.initials: D
authors.lastname: Bose
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, HBNI, Kolkata, India.
authors.forename: Malay
authors.initials: M
authors.lastname: Patra
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, HBNI, Kolkata, India. Electronic address: abhijit.chakrabarti@saha.ac.in.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
mesh_terms: D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D055550:Protein Stability; D013049:Spectrin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000737:chemistry; D014508:Urea / Q000737:chemistry
chemical_list: D018832:Molecular Chaperones; D013049:Spectrin; D014364:Tryptophan; D014508:Urea
keywords: Chaperone; Conformations; Erythroid & Non-erythroid Spectrin; Fluorescence; Oligomerization; Stability