title: Analgesic, Anti-Inflammatory, and Antioxidant Activities ofByrsonima duckeanaW. R. Anderson (Malpighiaceae).
abstract: Background.Byrsonimais a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless,Byrsonima duckeanaW. R. Anderson is an Amazonian species almost not studied.Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities ofByrsonima duckeanaleaves.Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays.Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples.B. duckeanawas able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in the formalin test by 57%. In the acetic acid-induced writhing test, the chloroform (FCL) and ethyl acetate (FEA) fractions were the most active samples. FEA was selected for the hot plate test, where all the dosages tested (5, 50, and 200 mg·kg-1) showed significant analgesic activity.Conclusion.B. duckeanahas interesting analgesic and antioxidant activities, due to its high phenolic content, especially phenolic acids.
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil; Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Maria Christina Dos Santos
authors.initials: MC
authors.lastname: Verdam
authors.affiliation: Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Fernanda
authors.identifier: 0000-0001-9528-746X
authors.initials: F
authors.lastname: Guilhon-Simplicio
authors.affiliation: Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Kleyton Cardoso
authors.initials: KC
authors.lastname: de Andrade
authors.affiliation: Center of Biological and Health Sciences, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
authors.forename: Karina Lorena Meira
authors.initials: KL
authors.lastname: Fernandes
authors.affiliation: Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Tallita Marques
authors.initials: TM
authors.lastname: Machado
authors.affiliation: Department of Chemistry, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Felipe Moura Araújo
authors.identifier: 0000-0002-1809-1372
authors.initials: FM
authors.lastname: da Silva
authors.affiliation: Department of Chemistry, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Mayane Pereira
authors.initials: MP
authors.lastname: de Souza
authors.affiliation: DeMpSter Mass Spectrometry Group, Amazonas State University, Manaus, AM, Brazil.
authors.forename: Hector Henrique Ferreira
authors.initials: HH
authors.lastname: Koolen
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Cristiane da Silva
authors.identifier: 0000-0002-5170-5727
authors.initials: CD
authors.lastname: Paula
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Beatriz Cristina Konopatzki
authors.identifier: 0000-0002-2766-2368
authors.initials: BC
authors.lastname: Hirota
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Vinícius Bednarczuk
authors.identifier: 0000-0001-7821-7742
authors.initials: VB
authors.lastname: de Oliveira
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Cristina Mayumi Sasaki
authors.initials: CM
authors.lastname: Miyazaki
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Milena
authors.initials: M
authors.lastname: Kalegari
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Marilis Dallarmi
authors.initials: MD
authors.lastname: Miguel
authors.affiliation: Center of Biological and Health Sciences, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
authors.forename: Patricia Maria
authors.initials: PM
authors.lastname: Stuelp-Campelo
authors.affiliation: Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Obdulio Gomes
authors.initials: OG
authors.lastname: Miguel
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004487:Edema / Q000188:drug therapy; D008297:Male; D029581:Malpighiaceae / Q000737:chemistry*; D051379:Mice; D010146:Pain / Q000188:drug therapy; D010936:Plant Extracts / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts