title: Relationship of DNA integrity toHRGC633T SNP and ART outcome in infertile couples.
abstract: The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome orHRGC633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n= 118) undergoingin vitrofertilisation (IVF) or intracytoplasmic sperm injection (ICSI). Sperm DNA integrity was analysed by flow cytometry using three fluorescent probes (acridine orange, monobromobimane and chromomycin A3). Principal component analysis (PCA) was used to identify the parameters that most influenced fertility. The relationships of sperm DNA integrity with seminal parameters,HRGC633T SNP and ART outcome were established using ANOVA andt-test. Sperm concentration and yield after preparation accounted for 27% of the total variance; sperm DNA integrity (%DFI and disulphide bonds) accounted for 16% of the variance in men from infertile couples. Sperm %DFI was significantly higher (P< 0.05) in older men than in younger men. A significant difference (P< 0.01) was observed in %DFI between smokers and non-smokers. Sperm %DFI was significantly higher (P< 0.01) in male factor infertility compared to either female factor or unknown factor infertility while free thiols were significantly higher (P< 0.01) in unknown infertility factor. No significant difference was observed between IVF success/failure in any of the seminal parameters studied. There was a tendency for protamine deficiency to be higher and disulphide concentration to be lower in men withHRG633T. Such assessments may provide additional useful information about the prognosis for ART outcome, although more research is needed before clinical guidelines can be provided.
authors.affiliation: Department of Clinical Sciences/Division of ReproductionSwedish University of Agricultural Sciences, Uppsala, Sweden.|Theriogenology LaboratoryAnimal Reproduction, Gynaecology & Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Kumaresan
authors.affiliation: Department of Clinical Sciences/Division of ReproductionSwedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Johannisson
authors.affiliation: Department of GeneticsImmunology and Pathology, and Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
authors.forename: Sarah
authors.initials: S
authors.lastname: Nordqvist
authors.affiliation: Department of GeneticsImmunology and Pathology, and Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Kårehed
authors.affiliation: Department of GeneticsImmunology and Pathology, Uppsala University, Uppsala, Sweden.
authors.forename: Helena
authors.initials: H
authors.lastname: Åkerud
authors.affiliation: Department of GeneticsImmunology and Pathology, and Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
authors.forename: Karin E
authors.initials: KE
authors.lastname: Lindgren
authors.affiliation: Department of Clinical Sciences/Division of ReproductionSwedish University of Agricultural Sciences, Uppsala, Sweden jane.morrell@slu.se.
authors.forename: Jane M
authors.initials: JM
authors.lastname: Morrell
mesh_terms: D000328:Adult; D000367:Age Factors; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011479:Protamines / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic / Q000706:statistics & numerical data; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D064424:Tobacco Use / Q000009:adverse effects; D000099053:Histidine-Rich Glycoprotein
chemical_list: D011479:Protamines; D011506:Proteins; D013438:Sulfhydryl Compounds; D000099053:Histidine-Rich Glycoprotein