title: The cardiovascular functions of salusin-β mediated by muscarinic receptors, glutamate receptors or L-type calcium channels within the rostral ventrolateral medulla of rats.
abstract: Salusin-α and salusin-β are newly identified bioactive peptides of 28 and 20 amino acids, respectively, that were initially predicted using in silico analyses and are widely distributed in the endocrine system, hematopoietic system, and central nervous system. The goal of our study was to investigate the cardiovascular effect of salusin-β microinjections into the rostral ventrolateral medulla (RVLM) in anesthetized rats and study their mechanism of action. Microinjection of the artificial cerebrospinal fluid (aCSF) into the RVLM did not affect the blood pressure (BP) or heart rate (HR) in anesthetized rats. Topical application of salusin-β into the RVLM produced a dose-dependently increase of BP in anesthetized rats. Microinjection of higher dose salusin-β produced significant tachycardia. Prior application of the L-NAME into the RVLM of rats did not alter the hypertension and tachycardia induced by intra-RVLM salusin-β. Notable, the cardiovascular functions elicited by intra-RVLM salusin-β were significantly decreased by pretreatment with Nic, KYN and atropine. In conclusion, the present study shows that the hypertension and tachycardia induced by intra-RVLM salusin-β might be partly mediated, at least in our opinion, by muscarinic receptors, glutamate receptors or L-type calcium channels.
authors.affiliation: Department of General Surgery, First Hospital of Lanzhou University, 1 DongGang West Road, Lanzhou, 730000, China.
authors.forename: Fu-Jia
authors.initials: FJ
authors.lastname: Xie
authors.affiliation: Department of General Surgery, First Hospital of Lanzhou University, 1 DongGang West Road, Lanzhou, 730000, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chai
authors.affiliation: Department of Clinical Laboratory, San Ai Tang Hospital, 74 Jing-Ning Road, Lanzhou, 730030, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, First Hospital of Lanzhou University, 1 DongGang West Road, Lanzhou, 730000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Intensive Care Unit, Lanzhou University Second Hospital, Lanzhou, 730000, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Cai
authors.affiliation: Department of Clinical Laboratory, San Ai Tang Hospital, 74 Jing-Ning Road, Lanzhou, 730030, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of General Surgery, First Hospital of Lanzhou University, 1 DongGang West Road, Lanzhou, 730000, China.
authors.forename: Nong
authors.identifier: http://orcid.org/0000-0001-5349-6140
authors.initials: N
authors.lastname: Cao
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D002320:Cardiovascular Physiological Phenomena / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D006973:Hypertension / Q000139:chemically induced; D036341:Intercellular Signaling Peptides and Proteins / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects* / Q000502:physiology; D008845:Microinjections; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013610:Tachycardia / Q000139:chemically induced
chemical_list: D020746:Calcium Channels, L-Type; D036341:Intercellular Signaling Peptides and Proteins; D017470:Receptors, Glutamate; D011976:Receptors, Muscarinic; C528274:salusin-beta, rat; D001285:Atropine
keywords: microinjection; rat; rostral ventrolateral medulla; salusin