title: Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are a common tool in regenerative medicine. The nanoscale extracellular vesicles (nEVs) secreted by these cells were recently brought up to light thanks to their therapeutic potential. In this study, we assessed the in vitro behaviour of human umbilical vein endothelial cells (HUVECs) exposed to nEVs derived from human umbilical cord mesenchymal stem cells (hUC-MSCs). Nanoscale extracellular vesicles were isolated and characterized by NanoSight® and flow cytometry. HUVECs were stimulated with various concentrations of nEVs. To assess nEV interactions with HUVECs, confocal microscopy and angiogenesis assay were performed. The use of nEVs derived from hUC-MSCs was able to produce positive outcomes on HUVECs by acting on their angiogenic potential.
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.
authors.forename: G
authors.initials: G
authors.lastname: Dostert
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.
authors.forename: A-S
authors.initials: AS
authors.lastname: Willemin
authors.affiliation: UMR 7039 CNRS-Université de Lorraine CRAN, Vandœuvre-lès-Nancy, France.
authors.forename: V
authors.initials: V
authors.lastname: Jouan-Hureaux
authors.affiliation: FR BMCT 3209, CNRS-INSERM-UL-CHRU de Nancy, Vandœuvre-lès-Nancy, France.|INSERM U1116, Vandœuvre-lès-Nancy, France.
authors.forename: H
authors.initials: H
authors.lastname: Louis
authors.affiliation: FR BMCT 3209, CNRS-INSERM-UL-CHRU de Nancy, Vandœuvre-lès-Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Hupont
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.|CHRU de Nancy, Vandœuvre-lès-Nancy, France.
authors.forename: P
authors.initials: P
authors.lastname: Gillet
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.|Faculté de Pharmacie, Nancy, France.
authors.forename: P
authors.initials: P
authors.lastname: Menu
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.|CHRU de Nancy, Vandœuvre-lès-Nancy, France.
authors.forename: V
authors.initials: V
authors.lastname: Decot
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.|CHRU de Nancy, Vandœuvre-lès-Nancy, France.
authors.forename: V
authors.initials: V
authors.lastname: Moby
authors.affiliation: UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France.|Faculté de Pharmacie, Nancy, France.
authors.forename: É
authors.initials: É
authors.lastname: Velot
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D000067128:Extracellular Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D018919:Neovascularization, Physiologic; D010316:Particle Size; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D043925:Angiogenesis Inducing Agents
keywords: HUVECs; Nanoscale extracellular vesicles; angiogenesis; human umbilical cord MSCs