title: Structural effect of Fe3O4nanoparticles on peroxidase-like activity for cancer therapy.
abstract: Ferromagnetic nanoparticles (Fe3O4NPs) have been proven to have the intrinsic peroxidase-like activity. This property has been used for analyte detection, tumor tissue visualization, and cancer therapy, etc. However, the effect of particle structure and morphology on its peroxidase-like activity has been rarely reported. In this work, we fabricated Fe3O4nanoparticles with different structures (nanoclusters, nanoflowers, and nanodiamonds) by facilely tuning the pH values in the hydrothermal reaction. Their in vitro peroxidase-like activity was evaluated via chromogenic reaction of 3,3',5,5'-tetramethylbenzidine (TMB) by the reduction of H2O2to H2O. It was found the nanostructures had a great influence on their peroxidase-like activity, following the order of nanoclusters>nanoflowers>nanodiamonds. With this activity, the peroxidase-like activity of Fe3O4NPs was used for cancer therapy with the addition of low-concentration H2O2. The cancer cell-killing activity was due to the intracellular generated reactive oxygen species (ROS) after endocytosis of Fe3O4NPs into the Hela cells. It was interesting that the cell killing ability of these three kinds of Fe3O4NPs was not consistent with the in vitro enzyme-like activity. It was deduced that the cell endocytosis of the nanoparticles along with their enzyme-like activity co-determined their cancer cell-killing performance.
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Fu
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China.
authors.forename: Anhui
authors.initials: A
authors.lastname: Qi
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Beijing Key Laboratory of Bioelectromagnetism, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address: chenchf@mail.iee.ac.cn.
authors.forename: Chuanfang
authors.initials: C
authors.lastname: Chen
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, PR China. Electronic address: lilinlin@binn.cas.cn.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
mesh_terms: D001560:Benzidines / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D052203:Ferrosoferric Oxide / Q000737:chemistry / Q000494:pharmacology*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018716:Molecular Mimicry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000819:agonists* / Q000378:metabolism; D014867:Water
chemical_list: D001560:Benzidines; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D014867:Water; C029569:diacetyldichlorofluorescein; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D009195:Peroxidase; D052203:Ferrosoferric Oxide
keywords: Cancer cell; Ferromagnetic nanoparticles; Nanostructure; Nanozyme