title: Immobilization of ω-transaminase by magnetic PVA-Fe3O4nanoparticles.
abstract: ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe3O4nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe3O4nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe3O4nanoparticles was located between 30-40 nm ω-TA was immobilized onto magnetic PVA-Fe3O4nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6-8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Jia
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhong
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
keywords: Chiral amine; Immobilization; Magnetic PVA-Fe3O4 nanoparticles; ω-transaminase