title: Effects of organic loading rate on biogas production from macroalgae: Performance and microbial community structure.
abstract: Macroalgae biomass has been considered as a promising feedstock for biogas production. In order to improve the efficiency of anaerobic digestion (AD) of macroalgae, semi-continuous fermentation was conducted to examine the effects of organic loading rate (OLR) on biogas production from Macrocystis pyrifer. Results showed that, under OLRs of 1.37, 2.74, 4.12 and 6.85kgVSsubstrate/(m3·d), the average unit biogas yields were 438.9, 477.3, 480.1 and 188.7mL/(gVSsubstrated), respectively. It indicated that biogas production was promoted by the increased OLR in an appropriate range while inhibited by the OLR beyond the appropriate range. The investigation on physical-chemical parameters revealed that unfavorable VFAs concentration, pH and salinity might be the main causes for system failure due to the overrange OLR, while the total phenols failed to reach the inhibitory concentration. Microbial community analysis demonstrated that several bacterial and archaeal phyla altered with increase in OLR apparently.
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Meng-Ting
authors.initials: MT
authors.lastname: Sun
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Fan
authors.affiliation: Jizhou Hebei Middle School, Hengshui, Hebei 053200, PR China.
authors.forename: Xiao-Xian
authors.initials: XX
authors.lastname: Zhao
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Shan-Fei
authors.initials: SF
authors.lastname: Fu
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: He
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: M R K
authors.initials: MRK
authors.lastname: Manasa
authors.affiliation: Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China. Electronic address: guorb@qibebt.ac.cn.
authors.forename: Rong-Bo
authors.initials: RB
authors.lastname: Guo
mesh_terms: D000693:Anaerobiosis; D001105:Archaea; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D012624:Seaweed
chemical_list: D056804:Biofuels
keywords: Anaerobic digestion; Biogas production; Macroalgae; Microbial community; Organic loading rate