title: Fluorescence monitoring of the effect of oxidized lipids on the process of protein fibrillization.
abstract: The kinetics of lysozyme and insulin amyloid formation in the presence of the oxidized phospholipids (oxPLs) was investigated using Thioflavin T fluorescence assay. The kinetic parameters of fibrillization process (lag time and apparent rate constant) have been determined upon varying the following experimental parameters: the type of lipid assemblies (premicellar aggregates and lipid bilayer vesicles), pH, temperature and lipid-to-protein molar ratio. It was found that oxPLs premicellar aggregates induced the more pronounced increase of the maximum Thioflavin T fluorescence, which is proportional to the extent of fibril formation, compared to the vesicles composed of the oxidized and unoxidized lipids. In contrast, the oxPLs, used as dispersions or included into vesicles, inhibited fibril nucleation and elongation under near-physiological conditions in vitro compared to liposomes containing unoxidized lipids. The results obtained provide deeper insight into the molecular mechanisms of the oxidative stress-modulated conformational diseases, and could be employed for the anti-amyloid drug development.
authors.affiliation: Department of Nuclear and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv 61022, Ukraine. Department of Nuclear and Medical Physics, V.N. Karazin Kharkiv National University, 12-38 Aeroflotska Str., Kharkiv 61031, Ukraine.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Vus
authors.forename: Rohit
authors.initials: R
authors.lastname: Sood
authors.forename: Galyna
authors.initials: G
authors.lastname: Gorbenko
authors.forename: Paavo
authors.initials: P
authors.lastname: Kinnunen
mesh_terms: D000682:Amyloid; D058227:Amyloidogenic Proteins; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry*; D013696:Temperature
chemical_list: D000682:Amyloid; D058227:Amyloidogenic Proteins; D008051:Lipid Bilayers; D008055:Lipids