title: Detection of DT-diaphorase Enzyme with a ParaCEST MRI Contrast Agent.
abstract: A responsive magnetic resonance (MRI) contrast agent has been developed that can detect the enzyme activity of DT-diaphorase. The agent produced different chemical exchange saturation transfer (CEST) MRI signals before and after incubation with the enzyme, NADH, and GSH at different pH values whereas it showed good stability in a reducing environment without enzyme.
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, 1306 E. University Blvd., Room 221, Tucson, Arizona, 85721-0041, USA.
authors.forename: Iman
authors.initials: I
authors.lastname: Daryaei
authors.affiliation: Department of Biomedical Engineering, University of Arizona, 1127 E James E. Rogers Way P.O. Box 210020, Tucson, AZ, 85721-0020, USA.
authors.forename: Kyle M
authors.initials: KM
authors.lastname: Jones
authors.affiliation: Department of Medical Imaging, University of Arizona, 1501 N. Campbell, P.O. Box 245067, Tucson, Arizona, 85724, USA.
authors.forename: Mark D
authors.identifier: http://orcid.org/0000-0002-8109-3995
authors.initials: MD
authors.lastname: Pagel
mesh_terms: D003287:Contrast Media / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008279:Magnetic Resonance Imaging; D009243:NAD / Q000737:chemistry; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003287:Contrast Media; D009243:NAD; D016660:NAD(P)H Dehydrogenase (Quinone); D005978:Glutathione
keywords: CEST MRI; NQO1; dt-diaphorase; molecular imaging; trimethyl lock