title: Integrin β1 Increases Stem Cell Survival and Cardiac Function after Myocardial Infarction.
abstract: Bone mesenchymal stem cells (BMSCs) transplantation is a promising therapeutic approach for myocardial infarction (MI), but its application is limited by poor viability of BMSCs. In this study, we aimed to improve the survival of BMSCs by lentivirus vector mediated overexpression of integrin β1.In vitrostudy showed that integrin β1 overexpression could facilitate the proliferation of BMSCs under oxygen glucose deprivation condition and regulated the expression of Caspase-3, Bax, Bcl-2, FAK, and ILK in BMSCs. Next, MI was induced in rat model and Igtb1BMSCs, NullBMSCs, or NatBMSCs were transplanted by intramyocardial injection. One week later, the survival of BMSCs was higher in Itgb1 BMSCs group than in other groups. Four weeks after transplantation, heart function was significantly improved in Igtb1BMSCs group compared to other groups. The expression levels of Caspase-3 and Bax were decreased while the expression levels of Bcl-2, FAK, ILK, and VEGF were increased in the cardiomyocytes of Igtb1BMSCs group compared to other groups. In conclusion, integrin β1 overexpression could increase the survival of BMSCs and improve the efficacy of transplanted BMSCs for MI treatment. The beneficial effects may be mediated by inhibiting the apoptosis of both transplanted BMSCs and cardiomyocytes through adhesion-mediated cell survival signaling.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Cardiology, Yunnan Fuwai Cardiovascular Disease Hospital Kunming City, China.
authors.forename: Qifan
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Department of Cardiac Rehabilitation, Shanxi Cardiovascular Hospital Taiyuan City, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Paediatrics, The First Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Harbin Medical University Harbin, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
keywords: adhesion; apoptosis; bone mesenchymal stem cell; integrin β1; myocardial infarction