title: The morphology ofGanoderma lucidummycelium in a repeated-batch fermentation for exopolysaccharide production.
abstract: The morphology ofGanoderma lucidumBCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace.G. lucidummycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morphology was disposed to EPS production in the shake flask and bioreactor, respectively. Seven RBF could be carried out in 500 mL flasks, and five repeated batches were performed in a 2 L bioreactor. Under RBF conditions, autolysis of pellet core in the shake flask and shaving off of the outer hairy region in the bioreactor were observed at the later stages of RBF (R4 for the shake flask and R6 for the bioreactor). The proposed strategy showed that the morphology ofG. lucidummycelium can withstand extended fermentation cycles.
authors.affiliation: Fermentation Centre, SIPBS, HW429, John Arburthnott Building (Hamnett Wing), 161 Cathedral Street, University of Strathclyde, Glasgow, G4 0RE, Scotland, UK.
authors.forename: Wan Abd Al Qadr Imad
authors.initials: WA
authors.lastname: Wan-Mohtar
authors.affiliation: Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors.forename: Safuan
authors.initials: S
authors.lastname: Ab Kadir
authors.affiliation: Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors.forename: Nazamid
authors.initials: N
authors.lastname: Saari
keywords: Ganoderma lucidum; Morphology; Ovoid pellet; Repeated-batch fermentation; Starburst-like pellet