title: Chromosomal Aberrations in Large Japanese Field Mice (Apodemus speciosus) Captured near Fukushima Dai-ichi Nuclear Power Plant.
abstract: Since the Fukushima Dai-ichi Nuclear Power Plant accident, radiation effects on nonhuman biota in the contaminated areas have been a major concern. Here, we analyzed the frequencies of chromosomal aberrations (translocations and dicentrics) in the splenic lymphocytes of large Japanese field mice (Apodemus speciosus) inhabiting Fukushima Prefecture. A. speciosus chromosomes 1, 2, and 5 were flow-sorted in order to develop A. speciosus chromosome-specific painting probes, and FISH (fluorescence in situ hybridization) was performed using these painting probes to detect the translocations and dicentrics. The average frequency of the translocations and dicentrics per cell in the heavily contaminated area was significantly higher than the frequencies in the case of the noncontaminated control area and the slightly and moderately contaminated areas, and this aberration frequency in individual mice tended to roughly increase with the estimated dose rates and accumulated doses. In all four sampling areas, the proportion of aberrations occurring in chromosome 2 was approximately >3 times higher than that in chromosomes 1 and 5, which suggests that A. speciosus chromosome 2 harbors a fragile site that is highly sensitive to chromosome breaks induced by cellular stress such as DNA replication. The elevated frequency of chromosomal aberrations in A. speciosus potentially resulting from the presence of a fragile site in chromosome 2 might make it challenging to observe the mild effect of chronic low-dose-rate irradiation on the induction of chromosomal aberrations in A. speciosus inhabiting the contaminated areas of Fukushima.
authors.affiliation: Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Kawagoshi
authors.affiliation: Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Shiomi
authors.affiliation: Tokyo Nuclear Services Co., Ltd. 1-3-5 Taito, Taito-ku, Tokyo 110-0016, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fuma
authors.affiliation: Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Doi
authors.affiliation: Center for Radiation Protection Knowledge, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Kawaguchi
authors.affiliation: Japan Wildlife Research Center, 3-3-7 Koutoubashi, Sumida-ku, Tokyo 130-8606, Japan.
authors.forename: Masanari
authors.initials: M
authors.lastname: Aoki
authors.affiliation: Japan Wildlife Research Center, 3-3-7 Koutoubashi, Sumida-ku, Tokyo 130-8606, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Kubota
authors.affiliation: Japan Wildlife Research Center, 3-3-7 Koutoubashi, Sumida-ku, Tokyo 130-8606, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Furuhata
authors.affiliation: Japan NUS Co., Ltd, 7-5-25 Nishi-shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Shigemura
authors.affiliation: Japan NUS Co., Ltd, 7-5-25 Nishi-shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Mizoguchi
authors.affiliation: Forestry and Forest Products Research Institute , 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Yamada
authors.affiliation: Department of Biology, Keio University , 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8521, Japan.
authors.forename: Morihiko
authors.initials: M
authors.lastname: Tomozawa
authors.affiliation: Faculty of Agriculture, University of Miyazaki , Kibana Campus, Miyazaki 889-2192, Japan.
authors.forename: Shinsuke H
authors.initials: SH
authors.lastname: Sakamoto
authors.affiliation: Department of Management and Planning, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Yoshihisa
authors.identifier: 0000-0002-2807-1535
authors.initials: Y
authors.lastname: Kubota
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D062406:Fukushima Nuclear Accident; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D051189:Murinae / Q000235:genetics*; D055848:Nuclear Power Plants