title: Evaluation of Chitosan-Based Films Containing Gelatin, Chondroitin 4-Sulfate and ZnO for Wound Healing.
abstract: In this work, chitosan-based films containing gelatin and chondroitin-4-sulfate (C4S) with and without ZnO particles were produced and tested in vitro to investigate their potential wound healing properties. Chitosans were produced from shrimp-head processing waste by alkaline deacetylation of chitin to obtain chitosans differing in molecular weight and degree of deacetylation (80 ± 0.5%). The film-forming solutions (chitosan, C4S and gelatin) and ZnO suspension showed no toxicity towards fibroblasts or keratinocytes. Chitosan was able to agglutinate red blood cells, and film-forming solutions induced no hemolysis. Film components were released into solution when incubated in PBS as demonstrated by protein and sugar determination. These data suggest that a stable, chitosan-based film with low toxicity and an ability to release components would be able to establish a biocompatible microenvironment for cell growth. Chitosan-based films significantly increased the percentage of wound healing (wound contraction from 65 to 86%) in skin with full-thickness excision when compared with control (51%), after 6 days. Moreover, histological analysis showed increased granulation tissue in chitosan and chitosan/gelatin/C4S/ZnO films. Chitosan-based biopolymer composites could be used for improved biomedical applications such as wound dressings, giving them enhanced properties.
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil. thiago.cahu@ufpe.br.
authors.forename: Thiago B
authors.initials: TB
authors.lastname: Cahú
authors.affiliation: Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas (UNICAMP), Av. Limeira, 901, Piracicaba, 13414-018, SP, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Silva
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Raquel P F
authors.initials: RPF
authors.lastname: Silva
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Milena M
authors.initials: MM
authors.lastname: Silva
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Isabel R S
authors.initials: IRS
authors.lastname: Arruda
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Janilson F
authors.initials: JF
authors.lastname: Silva
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Romero M P B
authors.initials: RMPB
authors.lastname: Costa
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Suzan D
authors.initials: SD
authors.lastname: Santos
authors.affiliation: Department of Molecular Biology, Federal University of São Paulo (UNIFESP), Rua Três de Maio, 100, São Paulo, 04044-020, SP, Brazil.
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.affiliation: Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Professor Moraes Rego, 1235, Recife, PE, 50670-901, Brazil.
authors.forename: Ranilson S
authors.initials: RS
authors.lastname: Bezerra
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D014945:Wound Healing / Q000187:drug effects*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin; D002809:Chondroitin Sulfates; D048271:Chitosan; D015034:Zinc Oxide
keywords: Antimicrobial; Biocompatibility; Biopolymer dressing; Waste biotechnology; Zinc oxide