title: Understanding the role of 3-O-Acetyl-11-keto-β-boswellic acid in conditions of oxidative-stress mediated hepatic dysfunction during benzo(a)pyrene induced toxicity.
abstract: The present study was planned to see whether 3-O-Acetyl-11- keto-β-boswellic acid has any protective effects against benzo(a)pyrene (BaP) induced toxicity or not. In vitro studies show concentration dependent linear association of radical scavenging activity of AK which is comparable to ascorbic acid taken as reference compound. For in vivo studies, the animals were divided randomly into five groups which included a) normal control, b) vehicle treated (olive oil), c) BaP treated, d) AK treated and e) AK + BaP (combined treated). BaP was administered at a dose of 50mg/kg in olive oil twice a week orally for 4 weeks and AK (50mg/kg) was given in olive oil thrice a week for 4 weeks before and after BaP exposure. BaP treated animals showed a significant increase (p < 0.001) in lipid peroxidation (LPO) and protein carbonyl contents (PCC) in hepatic tissue. Further, a significant increase (p < 0.001) in the liver marker enzymes as well as citrulline and nitric oxide levels in the hepatic tissue was also observed. Interestingly, AK when supplemented to BaP treated animals ameliorated the above said biochemical indices appreciately. The histopathological observations also showed appreciable improvement when BaP treated animals were supplemented with AK, thus emphasing the protective potential of AK.
authors.affiliation: Department of Biophysics, Panjab University Chandigarh, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Department of Biophysics, Panjab University Chandigarh, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Singh
authors.affiliation: Department of Biophysics, Panjab University Chandigarh, India.
authors.forename: Priti
authors.initials: P
authors.lastname: Bhardwaj
authors.affiliation: Department of Biophysics, Panjab University Chandigarh, India. Electronic address: dhatwalias@gmail.com.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Dhatwalia
authors.affiliation: Department of Biophysics, Panjab University Chandigarh, India. Electronic address: dhawan@pu.ac.in.
authors.forename: D K
authors.initials: DK
authors.lastname: Dhawan
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D029743:Boswellia / Q000737:chemistry*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008107:Liver Diseases / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D014315:Triterpenes / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: C518724:CML-1 plant extract; D010936:Plant Extracts; D014315:Triterpenes; D001564:Benzo(a)pyrene; C054625:boswellic acid
keywords: 3-O-Acetyl-11-keto-β-boswellic acid; Benzo(a)pyrene; Free radicals; Histopathology; Oxidative stress