title: Stimulation of HIV-specific T cell clonotypes using allogeneic HLA.
abstract: We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested. Allorecognition was associated with IFNγ cytokine production, CD137 upregulation and cytotoxicity, suggesting high avidity allo-stimulation. Allo-HLA recognition by HIV-specific T cells was specific to the HIV target peptide/HLA restriction and TCR TRBV usage of the T cells. HIV-specific T cells do crossreact against allogeneic HLA molecules in an epitope and TRBV specific manner. Therefore allo-HLA stimulation could be exploited to induce or augment HIV-specific T cell responses.
authors.affiliation: Department of Clinical Immunology, Fiona Stanley Hospital, Perth, Australia; Pathwest Laboratory Medicine, Perth, Australia; Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia.
authors.forename: Coral-Ann
authors.initials: CA
authors.lastname: Almeida
authors.affiliation: Department of Immunohaematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Paula
authors.initials: P
authors.lastname: van Miert
authors.affiliation: Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia.
authors.forename: Kane
authors.initials: K
authors.lastname: O'Driscoll
authors.affiliation: Department of Immunohaematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Yvonne M
authors.initials: YM
authors.lastname: Zoet
authors.affiliation: Institute for Immunology and Infectious Diseases, Murdoch University, Perth, Australia.
authors.forename: Abha
authors.initials: A
authors.lastname: Chopra
authors.affiliation: Institute for Immunology and Infectious Diseases, Murdoch University, Perth, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Watson
authors.affiliation: Department of Clinical Immunology, Fiona Stanley Hospital, Perth, Australia; Pathwest Laboratory Medicine, Perth, Australia.
authors.forename: Dianne
authors.initials: D
authors.lastname: de Santis
authors.affiliation: Pathwest Laboratory Medicine, Perth, Australia; Department of Clinical Immunology, Royal Perth Hospital, Perth, Australia.
authors.forename: Campbell
authors.initials: C
authors.lastname: Witt
authors.affiliation: Pathwest Laboratory Medicine, Perth, Australia; Institute for Immunology and Infectious Diseases, Murdoch University, Perth, Australia; Department of Clinical Immunology, Royal Perth Hospital, Perth, Australia.
authors.forename: Mina
authors.initials: M
authors.lastname: John
authors.affiliation: Department of Immunohaematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Frans H J
authors.initials: FHJ
authors.lastname: Claas
authors.affiliation: Department of Clinical Immunology, Fiona Stanley Hospital, Perth, Australia; Pathwest Laboratory Medicine, Perth, Australia; Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia; Institute for Immunology and Infectious Diseases, Murdoch University, Perth, Australia. Electronic address: Lloyd.dorsogna@health.wa.gov.au.
authors.forename: Lloyd J
authors.initials: LJ
authors.lastname: D'Orsogna
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D015331:Cohort Studies; D003429:Cross Reactions; D015488:HIV Antigens / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology; D015234:HLA-A Antigens / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000276:immunology
chemical_list: D015488:HIV Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D011948:Receptors, Antigen, T-Cell; D054301:gag Gene Products, Human Immunodeficiency Virus
keywords: Allostimulation; Crossreactivity; HIV; HLA; Memory T cells; Vaccine