title: Dual genetic absence of STAT6 and IL-10 does not abrogate anti-hyperglycemic effects of Schistosoma mansoni in streptozotocin-treated diabetic mice.
abstract: Schistosoma mansoni (Sm) is known to exert protective effects against various allergic and autoimmune disorders. It has been reported that this parasite protects NOD mice from spontaneous type 1 diabetes (T1D) and ameliorates streptozotocin (STZ)-induced T1D in wild-type mice. Here, we tried to clarify the anti-diabetic mechanisms of Sm in the latter model. Sm infection partially prevented the degradation of pancreatic islets and hyperglycemia in multiple low-dose (MLD) STZ-treated mice. Neither Treg cell depletion nor genetic absences of IL-10 and/or STAT6 abrogated the anti-hyperglycemic effects of Sm. Among M2 macrophage markers, Arg-1 and Ym1, but not Retnla, remained up-regulated in the pancreatic lymph nodes and in the spleens of STAT6/IL-10 double deficient (DKO) mice. Collectively, it is suggested that Sm exerts anti-diabetic effects on this experimental T1D model via Treg/IL-4/IL-13/IL-10-independent mechanisms. Augmented expressions of Arg-1 and Ym1 in the lymphoid organs adjacent to pancreas may be relevant to the anti-diabetic effects of Sm.
authors.affiliation: Department of Immunology and Parasitology, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. Electronic address: yosada@med.uoeh-u.ac.jp.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Osada
authors.affiliation: Department of Immunology and Parasitology, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Fujiyama
authors.affiliation: Department of Immunology and Parasitology, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Kamimura
authors.affiliation: Department of Immunology and Parasitology, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors.forename: Tsukushi
authors.initials: T
authors.lastname: Kaji
authors.affiliation: Laboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Nakae
authors.affiliation: Animal Research Center, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo 160-0022, Japan.
authors.forename: Katsuko
authors.initials: K
authors.lastname: Sudo
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishiwata
authors.affiliation: Department of Immunology and Parasitology, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kanazawa
mesh_terms: D000818:Animals; D001700:Biomphalaria; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000469:parasitology / Q000517:prevention & control; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000235:genetics* / Q000469:parasitology; D005786:Gene Expression Regulation; D007274:Injections, Intraperitoneal; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D018793:Interleukin-13 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D007515:Islets of Langerhans / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D050800:STAT6 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D012555:Schistosomiasis mansoni / Q000097:blood / Q000150:complications*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology; D013311:Streptozocin / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D001786:Blood Glucose; D018793:Interleukin-13; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; D016753:Interleukin-10; D015847:Interleukin-4; D013311:Streptozocin
keywords: Cytokine; Diabetes; Helminth; Immunomodulation; Schistosoma mansoni; Streptozotocin