title: Expression of cancer/testis antigens in salivary gland carcinomas with reference to MAGE-A and NY-ESO-1 expression in adenoid cystic carcinoma.
abstract: OBJECTIVE
  Cancer/testis antigens (CTAs) are detected in cancer cells but not in healthy normal tissues, with the exception of gametogenic tissues. CTAs are highly immunogenic proteins, and thus represent ideal targets for cytotoxic T-lymphocyte-mediated specific immune therapy. The aim of this study was to screen CTA expression in various types of salivary gland carcinoma and to clarify clinicopathological significance of MAGE-A and NY-ESO-1 expression in adenoid cystic carcinomas (AdCCs) of the salivary gland, which is one of the most common salivary gland carcinomas, and usually has a fatal outcome.
  
  
  RESULTS
  We used immunohistochemistry to examine the expression of four CTAs (MAGE-A, NY-ESO-1, CT7, and GAGE7) in various types of salivary gland carcinoma (n = 95). When carcinoma cases were divided into low-grade and intermediate/high-grade types, NY-ESO-1 and CT7 were expressed more frequently in intermediate/high-grade carcinomas. We then focused on MAGE-A and NY-ESO-1 expression in a large cohort of adenoid cystic carcinomas (AdCCs) (n = 46). MAGE-A and NY-ESO-1 were frequently expressed in AdCC; specifically, MAGE-A was expressed in >60% of the AdCC cases. MAGE-A expression and tumour site (minor salivary gland) were identified as independent risk factors for locoregional tumour recurrence.
  
  
  CONCLUSIONS
  These findings suggest that CTAs may be expressed in a variety of salivary gland carcinomas, especially in those with higher histological grades. In addition, MAGE-A, which is frequently expressed in AdCC cases, may be a useful prognostic factor for poorer locoregional recurrence-free survival.
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.|Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Beppu
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Fujii
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Saida
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Takino
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Masaki
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Murase
authors.affiliation: Pathology Division, Shizuoka Cancer Centre, Nagaizumi, Shizuoka, Japan.
authors.forename: Kimihide
authors.initials: K
authors.lastname: Kusafuka
authors.affiliation: Department of Head and Neck Surgery, Shizuoka Cancer Centre, Nagaizumi, Shizuoka, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Iida
authors.affiliation: Department of Head and Neck Surgery, Shizuoka Cancer Centre, Nagaizumi, Shizuoka, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Onitsuka
authors.affiliation: Department of Pathology and Molecular Diagnostics, Aichi Cancer Centre Hospital, Nagoya, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yatabe
authors.affiliation: Department of Head and Neck Surgery, Aichi Cancer Centre Hospital, Nagoya, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanai
authors.affiliation: Department of Head and Neck Surgery, Aichi Cancer Centre Hospital, Nagoya, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Ijichi
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Murakami
authors.affiliation: Department of Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Hiroshi
authors.identifier: http://orcid.org/0000-0001-6157-636X
authors.initials: H
authors.lastname: Inagaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis; D008875:Middle Aged; D016016:Proportional Hazards Models; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C104948:CTAG1B protein, human; D008565:Membrane Proteins
keywords: adenoid cystic carcinoma; cancer/testis antigens; immunohistochemistry; salivary gland carcinomas