title: The Benefit of Microskin in Combination With Autologous Keratinocyte Suspension to Treat Full Skin Loss In Vivo.
abstract: Patients with extensive deep burns often lack enough autologous skin to cover the wounds. This study explores a new method using microskin in combination with autologous keratinocytes in the treatment of extensive deep burn. Wounds in the combination group were treated with automicroskin at an area expansion ratio of 20:1 (wound area to automicroskin area) and autologous keratinocyte suspension, which were compared with the following treatments: no autotransplant, only allografts (control group); autologous keratinocyte suspension only (keratinocyte only group); automicroskin at an area expansion ratio of 20:1 (20:1 group); and automicroskin at an area expansion ratio of 10:1 (10:1 group, positive control). The authors used epithelialization rate (epithelialized area on day 21 divided by original wound area), hematoxylin and eosin staining, laminin, and type IV collagen immunohistochemistry to assess wound healing. The epithelialization rate of combination group (74.2% ± 8.0%) was similar to that of 10: 1 group (84.3% ± 11.9%, P = .085) and significantly (P < .05) higher than that of 20:1 group (59.2% ± 10.8%), keratinocyte only group (53.8% ± 11.5%), and control group (22.7% ± 5.5%). The hematoxylin and eosin staining and immunohistochemistry showed the epithelialization in the combination group was better than that in the keratinocyte only group and control group. Microskin in combination with autologous keratinocyte suspension can promote the reepithelialization of full-thickness wounds and reduce the requirements for automircoskin, and it is a useful option in the treatment of extensive deep burns.
authors.affiliation: From the Department of Burns and Plastic Surgery, First Affiliated Hospital of People's Liberation Army General Hospital, Beijing, People's Republic of China.
authors.forename: Shang
authors.initials: S
authors.lastname: Yuru
authors.forename: Li
authors.initials: L
authors.lastname: Dawei
authors.forename: Shen
authors.initials: S
authors.lastname: Chuanan
authors.forename: Yin
authors.initials: Y
authors.lastname: Kai
authors.forename: Ma
authors.initials: M
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Longzhu
authors.forename: Zhao
authors.initials: Z
authors.lastname: Dongxu
authors.forename: Cheng
authors.initials: C
authors.lastname: Wenfeng
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology / Q000628:therapy*; D017690:Cell Transplantation; D004195:Disease Models, Animal; D005260:Female; D015603:Keratinocytes / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D016038:Skin Transplantation; D014182:Transplantation, Autologous; D014945:Wound Healing