title: Proteomic Profiling of Skin Fibroblasts as a Model of Schizophrenia.
abstract: Since many aspects of schizophrenia are also manifested at the peripheral level in proliferating cell types, this chapter describes the analysis of skin fibroblasts biopsied from living patients. The method focuses on cell culture and sample preparation for characterization of the model. The resulting cell extracts can be analysed by any number of proteomic techniques for identification of biomarker candidates. This approach could help to elucidate the molecular mechanisms associated with the pathophysiology of schizophrenia and provide a useful model for a new target and drug discovery.
authors.affiliation: Apitope Technology (Bristol) Ltd, Riverside Court, Beaufort Park, Chepstow, NP16 5UH, UK.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil. paulcguest@yahoo.com.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D015153:Blotting, Western; D018929:Cell Culture Techniques; D002455:Cell Division; D002458:Cell Fractionation / Q000379:methods; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D040901:Proteomics / Q000379:methods*; D012559:Schizophrenia / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000166:cytology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
keywords: Biomarker; Cellular model; Drug screening; Fibroblasts; Proteomics; Schizophrenia; Target identification