title: A Theoretical Study on Inhibition of Melanoma with Controlled and Targeted Delivery of siRNA via Skin Using SPACE-EGF.
abstract: Melanoma is a potentially lethal skin cancer with high mortality rate. Recently, the peptide-mediated transdermal delivery of small interference RNA (siRNA) emerges as a promising strategy to treat melanoma by inducing the apoptosis of tumor cells, but the related theoretical model describing the delivery of siRNA under the effect of SPACE-EGF, the growth inhibition of melanoma and the dynamic expanding of the bump on the skin due to the growth of melanoma has not been reported yet. In this article, a theoretical model is developed to describe the percutaneous siRNA delivery mediated by SPACE-EGF to melanoma and the growth inhibition of melanoma. The results present the spatial-temporal distribution of siRNA and the growth of melanoma under the inhibition of siRNA, which shows a good consistency with the experimental results. In addition, this model represents the uplift process of tumors on the skin surface. The model presented here is a useful tool to understand the whole process of the SPACE-EGF-mediated delivery of the siRNA to melanoma through skin, to predict the therapeutic effect, and to optimize the therapeutic strategy, providing valuable references for the treatment of melanoma.
authors.affiliation: Center for Biomedical Engineering, University of Science and Technology of China, Hefei, 230027, Anhui, China.|Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center for Biomedical Engineering, University of Science and Technology of China, Hefei, 230027, Anhui, China. wpdings@ustc.edu.cn.|Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, 230027, Anhui, China. wpdings@ustc.edu.cn.
authors.forename: Weiping
authors.initials: W
authors.lastname: Ding
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Renquan
authors.initials: R
authors.lastname: Ruan
authors.affiliation: Center for Biomedical Engineering, University of Science and Technology of China, Hefei, 230027, Anhui, China.|Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zou
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.|Department of Pharmacology, Anhui University of Chinese Medicine, Hefei, 230038, Anhui, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wei
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.
authors.forename: Longping
authors.initials: L
authors.lastname: Wen
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000008:administration & dosage* / Q000627:therapeutic use; D004815:Epidermal Growth Factor / Q000008:administration & dosage* / Q000627:therapeutic use; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000627:therapeutic use; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D057846:Cell-Penetrating Peptides; D034741:RNA, Small Interfering; D004815:Epidermal Growth Factor
keywords: Melanoma; Peptide carrier; Transdermal drug delivery; Tumor growth inhibition; siRNA