title: A Sialidase-Deficient Porphyromonas gingivalis Mutant Strain Induces Less Interleukin-1β and Tumor Necrosis Factor-α in Epi4 Cells Than W83 Strain Through Regulation of c-Jun N-Terminal Kinase Pathway.
abstract: Porphyromonas gingivalis is one of the major periodontal pathogens. In a previous study, a mouse abscess model showed that sialidase deficiency of P. gingivalis weakened its virulence, but the mechanism behind this observation remains unknown.
  
  
  
  A sialidase-deficient mutant strain (△PG0352) and a complemented strain (com△PG0352) were constructed. Epi4 cells were stimulated by wild-type strain P. gingivalis W83, △PG0352, or com△PG0352. Real-time polymerase chain reaction was carried out to detect expression of virulent genes in P. gingivalis and interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in epi4 cells. Activities of sialidase, gingipains, and lipopolysaccharide (LPS) were compared among the different P. gingivalis strains. Levels of IL-1β and TNF-α in the epi4 cells supernatant were detected by enzyme-linked immunosorbent assay and levels of p38, extracellular signal-regulated kinase, c-Jun N-terminal kinase (JNK), and phospho-c-Jun were detected by western blotting.
  
  
  
  Compared with P. gingivalis W83 and com△PG0352, activities of Kgp and Rgp gingipains and amount of LPS decreased in △PG0352, whereas there were no differences in LPS activity among these three strains. Level of phospho-JNK was lower in epi4 cells stimulated by △PG0352. △pG0352 induced less IL-1β and TNF-α and more IL-8 in epi4 cells; differences in IL-1β and TNF-α could not be detected after JNK blocking.
  
  
  
  A sialidase-deficient P. gingivalis mutant strain induces less IL-1β and TNF-α in epi4 cells than W83 strain through regulation of JNK pathway.
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Periodontics and Oral Medicine, University of Michigan, Ann Arbor, MI.
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Tong
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Periodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000378:metabolism; D005798:Genes, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D053583:Interleukin-1beta / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects / Q000235:genetics; D009154:Mutation; D009439:Neuraminidase / Q000172:deficiency*; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D037521:Virulence Factors; D048031:JNK Mitogen-Activated Protein Kinases; D009439:Neuraminidase; D003546:Cysteine Endopeptidases
keywords: Epithelial cells; JNK mitogen-activated protein kinases; Porphyromonas gingivalis; gingiva; neuraminidase