title: Field-Effect Transistor Biosensor Platform Fused with Drosophila Odorant-Binding Proteins for Instant Ethanol Detection.
abstract: Odorant-binding proteins (OBPs) have attracted considerable attention as sensing substrates for the development of olfactory biosensors. The Drosophila LUSH protein is an OBP and is known to bind to various alcohols. Technology that uses the LUSH protein has great potential to provide crucial information through odorant detection. In this work, the LUSH protein was used as a sensing substrate to detect the ethanol concentration. Furthermore, we fused the LUSH protein with a silicon-on-insulator (SOI)-based ion-sensitive field-effect transistor (ISFET) to measure the electrical signals that arise from molecular interactions between the LUSH and ethanol. A dual-gate sensing system for self-amplification of the signal resulting from the molecular interaction between the LUSH and ethanol was then used to achieve a much higher sensitivity than a conventional ISFET. In the end, we successfully detected ethanol at concentrations ranging between 0.001 and 1% using the LUSH OBP-fused ISFET olfactory sensor. The OBP-fused SOI-based olfactory ISFET sensor can lead to the development of handheld sensors for various purposes such as detecting toxic chemicals, narcotics control, testing for food freshness, and noninvasive diagnoses.
authors.affiliation: Department of Electronic Materials Engineering, Kwangwoon University , 20 Gwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea.
authors.forename: Cheol-Min
authors.identifier: http://orcid.org/0000-0003-3691-8107
authors.initials: CM
authors.lastname: Lim
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University , Seobu-ro, Jangan-gu, Suwon 440-746, Gyeonggi-do, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kwon
authors.affiliation: Department of Electronic Materials Engineering, Kwangwoon University , 20 Gwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea.
authors.forename: Won-Ju
authors.initials: WJ
authors.lastname: Cho
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D000431:Ethanol; D018035:Receptors, Odorant
chemical_list: D029721:Drosophila Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein; D000431:Ethanol
keywords: LUSH; dual-gate ISFET; ethanol; odorant-binding protein; olfactory biosensor