title: Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts.
abstract: Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using yellow light from light-emitting diode (LED) combined with HB on keloid fibroblasts (KFB)in vitro. Our results showed that HB-LED PDT treatment induced significant KFB apoptosis and decreased KFB cell viability. HB-LED PDT treatment lead to significant BAX upregulation and BCL-2 downregulation in KFB cells, which led to elevation of intracellular free Ca2+and activation of caspase-3. Our data provides preliminary evidence for the potential of HB-LED PDT as a therapeutic strategy for keloid.
authors.affiliation: Department of Dermatology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Dermatology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
authors.forename: Chunmin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Hematology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Plastic Surgery, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Central Research Laboratory, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
authors.forename: Tonggang
authors.initials: T
authors.lastname: Qi
authors.affiliation: Department of Dermatology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.affiliation: Department of Dermatology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China.; Department of Dermatology, Peking University International Hospital, Beijing 102206, China.
authors.forename: Gangwen
authors.initials: G
authors.lastname: Han
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D007627:Keloid; D008027:Light; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D010778:Photochemotherapy / Q000379:methods*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D011809:Quinones; D051028:bcl-2-Associated X Protein; D010569:Perylene; C064522:hypocrellin B
keywords: Hypocrellin B; apoptosis; keloid fibroblasts; photodynamic therapy