title: Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected withLeishmania (Leishmania) amazonensis.
abstract: A surface plasmon resonance- (SPR-) based recognition method applying H-2 Ld:Ig/peptides complexes for ex vivo monitoring cellular immune responses during murine infection withLeishmania (Leishmania) amazonensisis described. Lymphocytes from lesion-draining popliteal lymph nodes were captured on a carboxylated sensor chip surface previously functionalized with H-2 Ld:Ig (DimerX) protein bound to synthetic peptides derived from the COOH-terminal region of cysteine proteinase B ofL. (L.) amazonensis.In computational analysis, these peptides presented values of kinetic constants favorable to form complexes with H-2 Ldat neutral pH, with a Gibbs free energy ΔG° < 0. The assayed DimerX:peptide complexes presented the property of attaching to distinct T lymphocytes subsets, obtained from experimentally infected BALB/c mice, in each week of infection, thus indicating a temporal variation in specific T lymphocytes populations, each directed to a different COOH-terminal region-derived peptide. The experimental design proposed herein is an innovative approach for cellular immunology studies of a neglected disease, providing a useful tool for the analysis of specific T lymphocytes subsets.
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, IOC, Fiocruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil; Laboratório de Imunologia Clínica, Departamento de Análises Clınicas e Toxicológicas, Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Avenida Gustavo Cordeiro de Farias S/N, 59010-180 Natal, RN, Brazil.
authors.forename: Lenilton Silva
authors.initials: LS
authors.lastname: da Silveira-Júnior
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, IOC, Fiocruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Franklin
authors.initials: F
authors.lastname: Souza-Silva
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, IOC, Fiocruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Bernardo Acácio Santini
authors.initials: BA
authors.lastname: Pereira
authors.affiliation: Laboratório de Imunoparasitologia, IOC, Fiocruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Léa
authors.initials: L
authors.lastname: Cysne-Finkelstein
authors.affiliation: Laboratório de Imunologia Clínica, Departamento de Análises Clınicas e Toxicológicas, Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Avenida Gustavo Cordeiro de Farias S/N, 59010-180 Natal, RN, Brazil.
authors.forename: Geraldo Barroso
authors.initials: GB
authors.lastname: Cavalcanti Júnior
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, IOC, Fiocruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Carlos Roberto
authors.identifier: 0000-0001-8703-426X
authors.initials: CR
authors.lastname: Alves
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057056:Cysteine Proteases / Q000737:chemistry / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D007111:Immunity, Cellular; D007891:Leishmania / Q000276:immunology* / Q000472:pathogenicity; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D051379:Mice; D046912:Multiprotein Complexes / Q000276:immunology / Q000302:isolation & purification; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification; D020349:Surface Plasmon Resonance; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
chemical_list: D046912:Multiprotein Complexes; D010455:Peptides; D057056:Cysteine Proteases