title: Transforming growth factor-beta 1 or ascorbic acid are able to differentiate Wharton's jelly mesenchymal stem cells towards a smooth muscle phenotype.
abstract: Wharton's jelly mesenchymal stem cells (WJ-MSCs) are widely used in tissue engineering. In vascular engineering, the ability to obtain a vessel replacement with contractile smooth muscle cells (SMC) is a key factor. In this work, we demonstrated that WJ-MSCs differentiate towards a SMC phenotype with various stimulations in vitro and that the modification of redox state could be involved. WJ-MSCs were isolated from umbilical cords. After their expansion, the cells were stimulated with ascorbic acid (AA, 300 μM) or transforming growth factor (TGF)-β1 (1 to 5 ng/mL). SMC markers were analyzed by Western blot. Modification of redox state was evaluated by reactive oxygen species (ROS) production and glutathione (GSH) content measurements. TGF-β1 or AA-stimulated WJ-MSCs express early and intermediate SMC markers. TGF-β1 (5 ng/mL) modifies the redox state by a ROS production and a GSH content drop, while AA has no effect. This work showed that TGF-β1 and AA are effective SMC phenotype inducers to differentiate WJ-MSCs.
authors.affiliation: UMR 7365, CNRS-Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Vandœuvre-lès-Nancy, 54505, France.
authors.forename: B
authors.initials: B
authors.lastname: Mesure
authors.affiliation: UMR 7365, CNRS-Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Vandœuvre-lès-Nancy, 54505, France.
authors.forename: S
authors.initials: S
authors.lastname: Huber-Villaume
authors.affiliation: UMR 7365, CNRS-Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Vandœuvre-lès-Nancy, 54505, France.|Faculté de Pharmacie, Université de Lorraine, Nancy, 54000, France.
authors.forename: P
authors.initials: P
authors.lastname: Menu
authors.affiliation: UMR 7365, CNRS-Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Vandœuvre-lès-Nancy, 54505, France.|Faculté de Pharmacie, Université de Lorraine, Nancy, 54000, France.
authors.forename: É
authors.initials: É
authors.lastname: Velot
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D001807:Blood Vessel Prosthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D024510:Muscle Development; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D018384:Oxidative Stress; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D053773:Transforming Growth Factor beta1; D001205:Ascorbic Acid
keywords: TGF-β1; Wharton’s jelly-MSCs; ascorbic acid; oxidative stress; smooth muscle cells