title: STITCHER 2.0: primer design for overlapping PCR applications.
abstract: Overlapping polymerase chain reaction (PCR) is a common technique used by researchers in very diverse fields that enables the user to 'stitch' individual pieces of DNA together. Previously, we have reported a web based tool called STITCHER that provides a platform for researchers to automate the design of primers for overlapping PCR applications. Here we present STITCHER 2.0, which represents a substantial update to STITCHER. STITCHER 2.0 is a newly designed web tool that automates the design of primers for overlapping PCR. Unlike STITCHER, STITCHER 2.0 considers diverse algorithmic parameters, and returns multiple result files that include a facility for the user to draw their own primers as well as comprehensive visual guides to the user's input, output, and designed primers. These result files provide greater control and insight during experimental design and troubleshooting. STITCHER 2.0 is freely available to all users without signup or login requirements and can be accessed at the following webpage: www.ohalloranlab.net/STITCHER2.html.
authors.affiliation: Department of Biological Sciences, The George Washington University, Science and Engineering Hall 6000, 800 22nd St. N.W. Washington DC 20052, USA.|Institute for Neuroscience, The George Washington University, 636 Ross Hall, 2300 I St. N.W. Washington DC 20052, USA.
authors.forename: Damien M
authors.initials: DM
authors.lastname: O'Halloran
authors.affiliation: The School Without Walls, 2130 G St. N.W., Washington, DC 20037 USA.
authors.forename: Isabel
authors.initials: I
authors.lastname: Uriagereka-Herburger
authors.affiliation: Department of Biological Sciences, The George Washington University, Science and Engineering Hall 6000, 800 22nd St. N.W. Washington DC 20052, USA.|Institute for Neuroscience, The George Washington University, 636 Ross Hall, 2300 I St. N.W. Washington DC 20052, USA.
authors.forename: Katrin
authors.initials: K
authors.lastname: Bode
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012984:Software; D064878:Web Browser
chemical_list: D017931:DNA Primers