title: Utility of microfluidic devices to study the platelet-endothelium interface.
abstract: The integration of biomaterials and understanding of vascular biology has led to the development of perfusable endothelialized flow models, which have been used as valuable tools to study the platelet-endothelium interface under shear. In these models, the parameters of geometry, compliance, biorheology, and cellular complexity are varied to recapitulate the physical biology of platelet recruitment and activation under physiologically relevant conditions of blood flow. In this review, we summarize the mechanistic insights learned from perfusable microvessel models and discuss the potential utility as well as challenges of endothelialized microfluidic devices to study platelet function in the bloodstream in vitro.
authors.affiliation: a Biomedical Engineering, School of Medicine , Oregon Health and Science University , Portland , OR , USA.
authors.forename: Jevgenia
authors.identifier: http://orcid.org/0000-0001-9862-9285
authors.initials: J
authors.lastname: Zilberman-Rudenko
authors.affiliation: a Biomedical Engineering, School of Medicine , Oregon Health and Science University , Portland , OR , USA.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Sylman
authors.affiliation: a Biomedical Engineering, School of Medicine , Oregon Health and Science University , Portland , OR , USA.|c Division of Pediatric Hematology/Oncology , Oregon Health and Science University , Portland , OR , USA.
authors.forename: Kathleen S
authors.initials: KS
authors.lastname: Garland
authors.affiliation: a Biomedical Engineering, School of Medicine , Oregon Health and Science University , Portland , OR , USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Puy
authors.affiliation: b Institute for Nanobiotechnology (INBT) , Johns Hopkins University , Baltimore , MD , USA.|d Department of Materials Science and Engineering , Johns Hopkins University , Baltimore , MD , USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Wong
authors.affiliation: b Institute for Nanobiotechnology (INBT) , Johns Hopkins University , Baltimore , MD , USA.|d Department of Materials Science and Engineering , Johns Hopkins University , Baltimore , MD , USA.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Searson
authors.affiliation: a Biomedical Engineering, School of Medicine , Oregon Health and Science University , Portland , OR , USA.|c Division of Pediatric Hematology/Oncology , Oregon Health and Science University , Portland , OR , USA.
authors.forename: Owen J T
authors.initials: OJT
authors.lastname: McCarty
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001792:Blood Platelets / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D055806:Microvessels / Q000378:metabolism / Q000503:physiopathology; D008955:Models, Cardiovascular; D015539:Platelet Activation
keywords: Endothelial cell; VWF; shear; vessel barrier