title: Transparent ciprofloxacin-povidone antibiotic films and nanofiber mats as potential skin and wound care dressings.
abstract: Water insoluble monohydrochloride monohydrate free ciprofloxacin (Cipro) antibiotic was incorporated in polyvinylpyrrolidone (PVP) polymer matrix by using acetic acid co-solvent in water. The resultant solutions were cast into fully transparent antimicrobial films. Proper concentrations of acetic acid eliminated in situ crystallization of the antibiotic and the resultant phase separation upon solvent evaporation. The solutions could also be electrospun into nanofiber mats (non-transparent). Presence of residual PVP-bound acetic acid in dry PVP films induced unprecedented levels of plasticity (stretching capacity) and softness to the films. Additionally, PVP-bound acetic acid also acted as an antiseptic. Antibacterial properties of the films and fiber mats were confirmed on Escherichia coli and Bacillus subtilis (growth and viability). Films and nanofiber mats demonstrated promising wound resorption characteristics by using in vivo full-thickness excisional skin wound healing mice model. Nanofiber mats were resorbed much faster than transparent films. Wound exudate absorption in the films and resorption rate of the nanofiber mats were dependent on the starting acetic acid concentrations. The fact that PVP/Cipro solutions in aqueous acetic acid can be used either to produce transparent soft films or nanofiber mats renders this process highly suitable for the fabrication of new-generation potential dressings for wound management and care.
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Contardi
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: José A
authors.initials: JA
authors.lastname: Heredia-Guerrero
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Perotto
authors.affiliation: Nanobiointeractions and Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Valentini
authors.affiliation: Nanobiointeractions and Nanodiagnostics, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pompa
authors.affiliation: Department of Drug Discovery and Development, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Spanò
authors.affiliation: Department of Drug Discovery and Development, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Goldoni
authors.affiliation: Department of Drug Discovery and Development, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Bertorelli
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy.
authors.forename: Athanassia
authors.initials: A
authors.lastname: Athanassiou
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, Genova 16163, Italy. Electronic address: ilker.bayer@iit.it.
authors.forename: Ilker S
authors.initials: IS
authors.lastname: Bayer
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001412:Bacillus subtilis / Q000187:drug effects; D001458:Bandages; D002939:Ciprofloxacin / Q000008:administration & dosage*; D004926:Escherichia coli / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D057139:Nanofibers; D011205:Povidone / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014947:Wounds and Injuries / Q000382:microbiology / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D011205:Povidone
keywords: Acetic acid; Ciprofloxacin; Nanofibers; Polyvinylpyrrolidone; Povidone; Wound dressing