title: Affinity chromatography of a binder of 1-methyladenine, the maturation-inducing hormone for starfish oocytes.
abstract: Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary. They resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the maturation-inducing hormone for starfish oocytes. Putative 1-MeAde receptors have been suggested to be present on the oocyte surface, but not yet been characterized biochemically. As reported recently (T. Toraya, T. Kida, A. Kuyama, S. Matsuda, S. Tanaka, Y. Komatsu, T. Tsurukai, Biochem. Biophys. Res. Commun. 485 (2017) 41-46), it became possible to detect unknown 1-MeAde binders of starfish oocytes by immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection. We designed and synthesized water-soluble and insoluble polymer-bound 1-MeAde derivatives. A water-soluble polymer-bound 1-MeAde derivative, in which 1-MeAde is bound to dextran through an N6-substituent, triggered the germinal-vesicle breakdown toward follicle-free oocytes, dejellied oocytes, and denuded oocytes. This is consistent with the idea that putative 1-MeAde receptors are located on the cell surface of starfish oocytes. A water-insoluble polymer-bound 1-MeAde derivative, in which 1-MeAde is bound to Sepharose 4B through an N6-substituent, served as an effective affinity adsorbent for the partial purification of a 1-MeAde binder with Mrof 47.5 K that might be a possible candidate of the maturation-inducing hormone receptors of starfish oocytes.
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan. Electronic address: toraya@cc.okayama-u.ac.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Toraya
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuyama
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Masatoyo
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Ohmiya
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tanabe
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D005079:Excipients / Q000737:chemistry*; D005260:Female; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology; D013215:Starfish / Q000378:metabolism*
chemical_list: D005079:Excipients; C008407:1-methyladenine; D000225:Adenine
keywords: 1-Methyladenine; Affinity chromatography; Oocyte maturation; Receptor; Starfish