title: Immunohistochemical analysis of EGFR and hyaluronan in tongue cancer and the development of regional recurrence in patients initially diagnosed N0.
abstract: OBJECTIVE
  To investigate whether the extent of expression of hyaluronan (HA) and epidermal growth factor receptor (EGFR) in squamous cell carcinoma of the mobile tongue can predict the risk of cervical metastasis and survival.
  
  
  METHODS
  Retrospective histopathologic study.
  
  
  METHODS
  Surgical specimens from 64 patients who had undergone surgery for squamous cell carcinoma of the mobile tongue were assessed using immunohistochemistry to investigate the expression of HA and EGFR in the primary tumours, and the data were then correlated to cervical metastasis and survival.
  
  
  RESULTS
  There was a significant correlation between the intensity of HA staining and patient survival (p .024), and a weak correlation between the staining proportion of EGFR and the risk for regional recurrence (AUC 66).
  
  
  CONCLUSIONS
  This study indicates that immunoscoring using HA could be used to provide prognostic tools for tongue cancer, and that it might be of interest to study the prognostic properties of EGFR further concerning the risk for regional recurrence after the primary treatment.
authors.affiliation: a Department of Surgical Science , Uppsala University , Uppsala , Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Lindell Jonsson
authors.affiliation: b Department of Medical Biosciences , Umeå University , Umeå , Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Nylander
authors.affiliation: c Department of Clinical Sciences , Umeå University , Umeå , Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Hallén
authors.affiliation: a Department of Surgical Science , Uppsala University , Uppsala , Sweden.
authors.forename: Göran
authors.identifier: http://orcid.org/0000-0002-7760-246X
authors.initials: G
authors.lastname: Laurell
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology / Q000628:therapy; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D012189:Retrospective Studies; D012737:Sex Factors; D013548:Sweden / Q000453:epidemiology; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology* / Q000628:therapy; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D006820:Hyaluronic Acid; D066246:ErbB Receptors
keywords: Epidermal growth factor receptor expression; biomarkers; immunoscoring; oral tongue squamous cell carcinoma; prognostic factors