title: The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway.
abstract: The telomerase/telomere interacting protein PinX1 has been suggested as a tumor suppressor. However, the clinical and biological significance of PinX1 in human non-small cell lung cancer (NSCLC) is unclear.
  
  
  
  PinX1 gene/expression pattern and its association with NSCLC patient survival were analyzed in cBioportal Web resource and two cohorts of NSCLC samples. A series of in vivo and in vitro assays were performed to elucidate the function of PinX1 on NSCLC cells proliferation and underlying mechanisms.
  
  
  
  More frequency of gene PinX1 homozygous deletion and heterozygote deficiency was first retrieved from cBioportal Web resource. Low expression of PinX1 correlated with smoking condition, histological type, T stage, N stage, M stage and TNM stage, and was an independent predictor for overall survival in a learning cohort (n = 93) and a validation cohort (n = 51) of NSCLC patients. Furthermore, knockdown of PinX1 dramatically accelerated NSCLC cell proliferation and G1/S transition, whereas ectopic overexpression of PinX1 substantially inhibited cell viability and cell cycle transition in vitro and in vivo. p15/cyclin D1 pathway and BMP5 might contribute to PinX1-associated cell proliferation and cell cycle transition.
  
  
  
  The cost-effective expression of PinX1 could constitute a novel molecular predictor/marker for NSCLC management.
authors.affiliation: Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.|State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiao-Han
authors.initials: XH
authors.lastname: Jin
authors.affiliation: Department of Pathology, Cancer Center, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wei-Juan
authors.initials: WJ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.affiliation: Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China. jxzhangsysu@163.com.|Department of Oncology, The first Affiliated Hospital, Sun Yat-Sen University, No.58, Zhongshan Second Road, 510080, Guangzhou, China. jxzhangsysu@163.com.
authors.forename: Jia-Xing
authors.initials: JX
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor; D055417:Bone Morphogenetic Protein 5 / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism*; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000378:metabolism*; D030561:Databases, Nucleic Acid; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D008297:Male; D051379:Mice; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: C525814:BMP5 protein, human; D014408:Biomarkers, Tumor; D055417:Bone Morphogenetic Protein 5; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; C440808:PINX1 protein, human; D025521:Tumor Suppressor Proteins; D019938:Cyclin D1
keywords: BMP5; Cell cycle; Non-small cell lung cancer; P15; PinX1