title: Activation of Wnt3α/β-catenin signal pathway attenuates apoptosis of the cerebral microvascular endothelial cells induced by oxygen-glucose deprivation.
abstract: Brain microvascular endothelial cells (BMECs) play vital roles in cerebral ischemia, during which many signal pathways mediate BMECs apoptosis. In this study, we explored the potential role of Wnt3α/β-catenin signal in BMECs apoptosis induced by ischemia. Here, we found that oxygen-glucose deprivation (OGD) could induce apoptosis of BMECs with Wnt3a mRNA expression decrease. Meanwhile, activation Wnt3a/β-catenin signal with exogenous Wnt3α protein (100 ng/ml) or Lithium Chloride (LiCl, 4 mM) decreased significantly apoptosis of BMECs induced by OGD with increasing expression of Bcl-2 in the whole cell and β-catenin in the nucleus. But, inhibition Wnt3a/β-catenin signal with DKK1 (100 ng/ml) or 2.4-diamino quinazoline (DQ, 0.2 μM) increased apoptosis of BMECs with decreasing expression of Bcl-2. These results suggest that activation Wnt3α/β-catenin signal attenuate apoptosis of BMECs induced by ischemia.
authors.affiliation: Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Department of Human Anatomy, Histology and Embryology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
authors.forename: Jianshui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Human Anatomy, Xi'an Medical University, Xi'an, Shaanxi 710021, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Department of Human Anatomy, Qinghai University School of Medicine, Xining, Qinghai 810001, China.
authors.forename: Cunfang
authors.initials: C
authors.lastname: Qi
authors.affiliation: Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Department of Human Anatomy, Histology and Embryology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Yang
authors.affiliation: Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Department of Human Anatomy, Histology and Embryology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
authors.forename: Xinlin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China. Electronic address: liuy5599@mail.xjtu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000187:drug effects; D054022:Cerebrum / Q000098:blood supply*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000172:deficiency* / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D018021:Lithium Chloride / Q000494:pharmacology; D055806:Microvessels / Q000166:cytology; D010100:Oxygen / Q000378:metabolism*; D011799:Quinazolines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D060449:Wnt Signaling Pathway / Q000187:drug effects; D060508:Wnt3 Protein / Q000819:agonists / Q000378:metabolism*; D051176:beta Catenin / Q000819:agonists / Q000378:metabolism*
chemical_list: C495268:Ctnnb1 protein, rat; C531384:Dkk1 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D011799:Quinazolines; D060508:Wnt3 Protein; C555509:Wnt3 protein, rat; D051176:beta Catenin; C013474:2,4-diaminoquinazoline; D018021:Lithium Chloride; D005947:Glucose; D010100:Oxygen
keywords: Apoptosis; Brain microvascular endothelial cells (BMECs); Oxygen-glucose deprivation (OGD); Wnt3α/β-catenin signal