title: Protective effects of cerium oxide and yttrium oxide nanoparticles on reduction of oxidative stress induced by sub-acute exposure to diazinon in the rat pancreas.
abstract: Diazinon is a kind of organophosphorus (OP) compound that is broadly used against different species of insects and pests. Oxidative stress can occur at very early stages of diazinon exposure and the pancreas is one of the main target organs for toxicity by diazinon. The aim of this study was to evaluate the protective effects of cerium oxide nanoparticles (CeO2NPs) and yttrium oxide nanoparticles (Y2O3NPs) against the pancreatic damage from sub-acute exposure of diazinon. Diazinon at a dose of 70mg/kg/day was given through gavage to rats once a day. Along with diazinon, trace amounts of CeO2NPs and Y2O3NPs (35mg/kg and 45mg/kg per day, respectively) were administered by intraperitoneal injection once a day for 2 weeks. Animals weight and blood glucose were measured during the treatment, and oxidative stress biomarkers, diabetes physiology, function and viability of cells were investigated at the end of the treatment in serum and pancreas tissues. Apoptosis of islets was examined by the flow cytometry. The high blood glucose level and significant weight loss resulting from diazinon were modified as a result of the application of the NPs. A significant recovery in oxidative stress markers, pro-insulin, insulin, C-peptide, adenosine diphosphate/adenosine triphosphate (ATP/ADP) ratio, caspase-3 and -9 activities and apoptosis-necrosis in the islets was observed. In conclusion, administration of CeO2NPs or Y2O3NPs only or their combination with suitable and defined dose will help to overcome the consequences from oxidant agents.
authors.affiliation: Department of Occupational Health, Faculty of Health, Qom University of Medical Sciences, Qom, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Khaksar
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahban
authors.initials: M
authors.lastname: Rahimifard
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Baeeri
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran; Tehran University of Medical Sciences, International Campus, (TUMS-IC), Tehran 1417614411, Iran.
authors.forename: Faheem
authors.initials: F
authors.lastname: Maqbool
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Navaei-Nigjeh
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shokoufeh
authors.initials: S
authors.lastname: Hassani
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Shermineh
authors.initials: S
authors.lastname: Moeini-Nodeh
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Kebriaeezadeh
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran; Tehran University of Medical Sciences, International Campus, (TUMS-IC), Tehran 1417614411, Iran; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: Mohammad@TUMS.Ac.Ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdollahi
mesh_terms: D000818:Animals; D002563:Cerium / Q000008:administration & dosage / Q000494:pharmacology*; D003976:Diazinon / Q000008:administration & dosage* / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010179:Pancreas / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015019:Yttrium / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002563:Cerium; D015019:Yttrium; C030583:ceric oxide; C091417:yttria; D003976:Diazinon
keywords: CeO(2) nanoparticles; Diazinon; Oxidative stress; Pancreatic islets; Y(2)O(3) nanoparticles