title: Epithelial-mesenchymal transition, and collective and individual cell migration regulate epithelial changes in the amikacin-damaged organ of Corti.
abstract: Characterizing the microenvironment of a damaged organ of Corti and identifying the basic mechanisms involved in subsequent epithelial reorganization are critical for improving the outcome of clinical therapies. In this context, we studied the expression of a variety of cell markers related to cell shape, cell adhesion and cell plasticity in the rat organ of Corti poisoned with amikacin. Our results indicate that, after severe outer hair cell losses, the cytoarchitectural reorganization of the organ of Corti implicates epithelial-mesenchymal transition mechanisms and involves both collective and individual cell migratory processes. The results also suggest that both root cells and infiltrated fibroblasts participate in the homeostasis of the damaged epithelium, and that the flat epithelium that may emerge offers biological opportunities for late regenerative therapies.
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital Saint Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.|University of Montpellier, Montpellier, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Ladrech
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital Saint Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.|University of Montpellier, Montpellier, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Eybalin
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital Saint Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.|University of Montpellier, Montpellier, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Puel
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital Saint Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France. marc.lenoir@inserm.fr.|University of Montpellier, Montpellier, France. marc.lenoir@inserm.fr.
authors.forename: Marc
authors.identifier: http://orcid.org/0000-0003-0117-8487
authors.initials: M
authors.lastname: Lenoir
mesh_terms: D000583:Amikacin / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D009925:Organ of Corti / Q000187:drug effects* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000583:Amikacin
keywords: Adhesion proteins; Cochlea; Fibroblasts; Flat epithelium; Ototoxicity; Root cells