title: 5-HMF Attenuates Liver Fibrosis in CCl4-Plus-Alcohol-Induced Mice by Suppression of Oxidative Stress.
abstract: The aim of this study was to investigate the effects of 5-hydroxymethyl-2-furfural (5-HMF) on liver fibrosis induced by carbon tetrachloride (CCl4) and alcohol. Male ICR mice were treated with CCl4dissolved in olive oil (10% v/v, 2.5 μg/L) intraperitoneally (i.p.), and given at a dose of 2.5×10-5mg/kg B.W. twice a week for 7 wk. Concurrently, mice received drinking water with or without alcohol. The mice in treatment groups and positive control group were gavaged with 5-HMF (7.5, 15, and 30 mg/kg B.W.) or Huganpian (350 mg/kg B.W.) daily starting in the fourth week and lasting for 4 wk. The blood samples were analyzed for biochemical markers of hepatic injury and tissue samples were subjected for estimation of liver antioxidants and histopathological studies. The concentrations of HA (hyaluronic acid), LN (laminin), CIV (collagen type IV), and MDA (malondialdehyde), as well as the serum levels of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced by 5-HMF. On the other hand, enzymatic antioxidants SOD (superoxide dismutase), CAT (catalase) and GSH-Px (glutathione peroxidase) were markedly elevated in liver tissue treated with 5-HMF. Histopathological examination revealed that 5-HMF treatment noticeably prevented hepatocyte apoptosis, fatty degeneration and inflammatory cell infiltration on liver fibrosis induced by CCl4and alcohol. Hoechst 33258 staining also revealed hepatocyte apoptosis. 5-HMF could exert protective effects against liver injury and reduce liver fibrosis induced by CCl4and alcohol in mice.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University.
authors.forename: Xin-Yue
authors.initials: XY
authors.lastname: Han
authors.forename: Jun-Nan
authors.initials: JN
authors.lastname: Hu
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Wei
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Zheng
authors.forename: Ying-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000008:administration & dosage*; D002374:Catalase / Q000097:blood; D000431:Ethanol / Q000008:administration & dosage*; D005662:Furaldehyde / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D000431:Ethanol; D008315:Malondialdehyde; C008046:5-hydroxymethylfurfural; D002251:Carbon Tetrachloride; D005662:Furaldehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase