title: The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro.
abstract: Exosomes is a key component of cell paracrine secretion and can exert important effects in various disease models. However, the role of exosomes in neuron repair of spinal cord injury (SCI) has rarely been reported. In this study, Exosomes were isolated from cerebrospinal fluid of SCI and normal, and incubated neuron in vitro respectively to research its biological function in cell proliferation. The results demonstrated these exosomes all expressed CD9, CD63, CD81, Alix and Tsg101; however, only exosomes derived from cerebrospinal fluid of SCI could promote proliferation of neuron via ERK signaling pathway, and decrease cell apoptosis. Exosomes contain cytosolic content, including proteins, mRNAs and non-cording RNAs, and play a role in important biological function. Our research showed exosomes derived from cerebrospinal fluid of SCI, they can influence neuron cell proliferation in vitro, we did not observe these characters in exosome derived from normal cerebrospinal fluid.
authors.affiliation: a Department of Orthopaedics , The Third Hospital of HeBei Medical University , Shijiazhuang , China.
authors.forename: Fan-Long
authors.initials: FL
authors.lastname: Kong
authors.affiliation: b Department of Immunology and Rheumatology , The Second Hospital of HeBei Medical University , Shijiazhuang , China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wang
authors.affiliation: c Department of Anesthesiology , The Obstetrics and Gynecology Hospital of Shijiazhuang , Shijiazhuang , China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Li
authors.affiliation: a Department of Orthopaedics , The Third Hospital of HeBei Medical University , Shijiazhuang , China.
authors.forename: Hai-Xu
authors.initials: HX
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002555:Cerebrospinal Fluid / Q000166:cytology*; D055354:Exosomes / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D009474:Neurons / Q000166:cytology*; D051381:Rats; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid*
keywords: Spinal cord injury; cerebrospinal fluid; exosomes; neuron