title: Optimal Hotspots of Dynamic Surfaced-Enhanced Raman Spectroscopy for Drugs Quantitative Detection.
abstract: Surface-enhanced Raman spectroscopy (SERS) as a powerful qualitative analysis method has been widely applied in many fields. However, SERS for quantitative analysis still suffers from several challenges partially because of the absence of stable and credible analytical strategy. Here, we demonstrate that the optimal hotspots created from dynamic surfaced-enhanced Raman spectroscopy (D-SERS) can be used for quantitative SERS measurements. In situ small-angle X-ray scattering was carried out to in situ real-time monitor the formation of the optimal hotspots, where the optimal hotspots with the most efficient hotspots were generated during the monodisperse Au-sol evaporating process. Importantly, the natural evaporation of Au-sol avoids the nanoparticles instability of salt-induced, and formation of ordered three-dimensional hotspots allows SERS detection with excellent reproducibility. Considering SERS signal variability in the D-SERS process, 4-mercaptopyridine (4-mpy) acted as internal standard to validly correct and improve stability as well as reduce fluctuation of signals. The strongest SERS spectra at the optimal hotspots of D-SERS have been extracted to statistics analysis. By using the SERS signal of 4-mpy as a stable internal calibration standard, the relative SERS intensity of target molecules demonstrated a linear response versus the negative logarithm of concentrations at the point of strongest SERS signals, which illustrates the great potential for quantitative analysis. The public drugs 3,4-methylenedioxymethamphetamine and α-methyltryptamine hydrochloride obtained precise analysis with internal standard D-SERS strategy. As a consequence, one has reason to believe our approach is promising to challenge quantitative problems in conventional SERS analysis.
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.|Department of Chemistry, University of Science and Technology of China , Hefei 230026, China.
authors.forename: Xiunan
authors.initials: X
authors.lastname: Yan
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.|Department of Chemistry, University of Science and Technology of China , Hefei 230026, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.|Department of Chemistry, University of Science and Technology of China , Hefei 230026, China.
authors.forename: Xianghu
authors.initials: X
authors.lastname: Tang
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201204, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.
authors.forename: Shizhuang
authors.initials: S
authors.lastname: Weng
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.
authors.forename: Liangbao
authors.identifier: 0000-0002-6559-6947
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences , Hefei 230031, China.
authors.forename: Jinhuai
authors.initials: J
authors.lastname: Liu
mesh_terms: D006046:Gold / Q000737:chemistry; D013287:Illicit Drugs / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000032:analysis*; D053838:Scattering, Small Angle; D013059:Spectrum Analysis, Raman / Q000379:methods; D014363:Tryptamines / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D013287:Illicit Drugs; D014363:Tryptamines; D006046:Gold; C015293:indopan; D018817:N-Methyl-3,4-methylenedioxyamphetamine