title: Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.
abstract: We have built a series of Neurospora crassa strains containing alleles of green fluorescent protein (GFP) to provide a visual phenotype for investigating meiotic recombination. These strains provide a convenient means of screening the Neurospora knockout library for genes involved in genetic recombination. They permit rapid analysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for GFP. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.
authors.affiliation: School of Biological Sciences, Flinders University, 2100, Adelaide, 5001, SA, Australia.
authors.forename: Frederick J
authors.initials: FJ
authors.lastname: Bowring
authors.affiliation: School of Biological Sciences, Flinders University, 2100, Adelaide, 5001, SA, Australia.
authors.forename: P Jane
authors.initials: PJ
authors.lastname: Yeadon
authors.affiliation: School of Biological Sciences, Flinders University, 2100, Adelaide, 5001, SA, Australia. david.catcheside@flinders.edu.au.
authors.forename: David E A
authors.initials: DEA
authors.lastname: Catcheside
mesh_terms: D003434:Crossing Over, Genetic; D005656:Fungal Proteins / Q000235:genetics; D005785:Gene Conversion; D055786:Gene Knockout Techniques / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006579:Heterozygote; D008540:Meiosis; D009492:Neurospora crassa / Q000235:genetics*; D030781:Organisms, Genetically Modified; D011995:Recombination, Genetic
chemical_list: D005656:Fungal Proteins; D049452:Green Fluorescent Proteins
keywords: Crossing over; GFP; Meiosis; Neurospora; Octads; Recombination; Tetrad analysis