title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold.
abstract: The purpose of this study is to investigate electrospinning poly(L-lactic acid) (PLLA) nanofibrous scaffold with different contents of amorphous calcium phosphate (ACP), which is suitable for using in bone regeneration through sustained release of calcium and orthophosphate ions. Three groups of nanofibrous scaffolds, ACP-free PLLA, ACP-5 wt%/PLLA and ACP-10 wt%/PLLA, are developed and characterized by scanning electron microscopy and gel permeation chromatography. Calcium and phosphate colorimetric assay kits are used to test ions released from scaffold during hydrolytic degradation. The results show ACP-5 wt%/PLLA and ACP-10 wt%/PLLA scaffolds have relatively high degradation rates than ACP-free PLLA group. The bioactivity evaluation further reveals that ACP-5 wt%/PLLA scaffold presents more biocompatible feature with pre-osteoblast cells and significant osteogenesis ability of calvarial bone defect. Due to the facile preparation method, sustained calcium and orthophosphate release behavior, and excellent osteogenesis capacity, the presented ACP/PLLA nanofibrous scaffold has potential applications in bone tissue engineering.
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Niu
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology; National Engineering Laboratory for Digital and Material Technology of Stomatology; Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: Zhongning
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Siqian
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology; National Engineering Laboratory for Digital and Material Technology of Stomatology; Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology; National Engineering Laboratory for Digital and Material Technology of Stomatology; Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of New Ceramic and Fine Processing, Tsinghua University, Beijing 100084, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.|National Research Center for Rehabilitation Technical Aids, Beijing 100176, China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D002118:Calcium / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D008297:Male; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis; D010710:Phosphates / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D010710:Phosphates; D011091:Polyesters; C519480:amorphous calcium phosphate; C033616:poly(lactide); D002118:Calcium