title: Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients.
abstract: The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed. RAPD typing in strains with the same serotype revealed different genotypes and, on the contrary, most strains with a different serotype displayed the same amplification pattern. The resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients. A higher degree of antibiotic resistance and stronger biofilm production in common genotypes compared to rare ones and genetic homogeneity of the most resistant strains indicated the role of antibiotic pressure in acquiring resistant and more virulent strains in our hospital. In conclusion, genetic characterisation of P. aeruginosa strains using RAPD method was shown to be more accurate in epidemiological analyses than phenotyping.
authors.affiliation: Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Pekařská 53, 656 91, Brno, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Vaněrková
authors.affiliation: Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Pekařská 53, 656 91, Brno, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Mališová
authors.affiliation: Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Pekařská 53, 656 91, Brno, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Kotásková
authors.affiliation: Department of Clinical Microbiology, Faculty of Medicine, Masaryk University, and St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Holá
authors.affiliation: Department of Clinical Microbiology, Faculty of Medicine, Masaryk University, and St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Filip
authors.initials: F
authors.lastname: Růžička
authors.affiliation: Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Pekařská 53, 656 91, Brno, Czech Republic. tomas.freiberger@cktch.cz.|Department of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, and St. Anne's University Hospital, Brno, Czech Republic. tomas.freiberger@cktch.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Freiberger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019105:Random Amplified Polymorphic DNA Technique; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic Susceptibility; Antimicrobial Susceptibility Pattern; Common Genotype; Meropenem; Unique Genotype