title: NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.
abstract: In plants movement of the endoplasmic reticulum (ER) is dependent on the actin cytoskeleton. However little is known about proteins that link the ER membrane and the actin cytoskeleton. Here we identified a novel protein, NETWORKED 3B (NET3B), which is associated with the ER and actin cytoskeleton in vivo. NET3B belongs to a superfamily of plant specific actin binding proteins, the NETWORKED family. NET3B associates with the actin cytoskeleton in vivo through an N-terminal NET actin binding (NAB) domain, which has been well-characterized in other members of the NET family. A three amino acid insertion, Val-Glu-Asp, in the NAB domain of NET3B appears to lower its ability to localize to the actin cytoskeleton compared with NET1A, the founding member of the NET family. The C-terminal domain of NET3B links the protein to the ER. Overexpression of NET3B enhanced the association between the ER and the actin cytoskeleton, and the extent of this association was dependent on the amount of NET3B available. Another effect of NET3B overexpression was a reduction in ER membrane diffusion. In conclusion, our results revealed that NET3B modulates ER and actin cytoskeleton interactions in higher plants.
authors.affiliation: Department of Biosciences, Durham University, South road, Durham, DH1 3LE, UK.|College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Biosciences, Durham University, South road, Durham, DH1 3LE,UK.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hussey
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008840:Microfilament Proteins; C000627711:NET3B protein, Arabidopsis
keywords: Actin cytoskeleton; N. benthamiana.; NET superfamily; endomembrane system; endoplasmic reticulum