title: High-throughput system-wide engineering and screening for microbial biotechnology.
abstract: Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their experiments to the nanoliter and picoliter scale and the single-cell level. In this review, we summarize different high-throughput system-wide genome engineering and screening strategies for microbes. More specifically, we will emphasize the use of multiplex automated genome evolution (MAGE) and CRISPR/Cas systems for high-throughput genome engineering and the application of (lab-on-chip) nanoreactors for high-throughput single-cell or population screening.
authors.affiliation: Laboratory for Systems Biology, VIB Center for Microbiology, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Laboratory for Genetics and Genomics, KU Leuven, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Imec Life Science Technologies, Kapeldreef 75, B-3001 Leuven, Belgium.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Vervoort
authors.affiliation: Laboratory for Systems Biology, VIB Center for Microbiology, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Laboratory for Genetics and Genomics, KU Leuven, Gaston Geenslaan 1, B-3001 Leuven, Belgium.
authors.forename: Alicia Gutiérrez
authors.initials: AG
authors.lastname: Linares
authors.affiliation: Laboratory for Systems Biology, VIB Center for Microbiology, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Laboratory for Genetics and Genomics, KU Leuven, Gaston Geenslaan 1, B-3001 Leuven, Belgium.
authors.forename: Miguel
authors.initials: M
authors.lastname: Roncoroni
authors.affiliation: Imec Life Science Technologies, Kapeldreef 75, B-3001 Leuven, Belgium.
authors.forename: Chengxun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory for Systems Biology, VIB Center for Microbiology, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Laboratory for Genetics and Genomics, KU Leuven, Gaston Geenslaan 1, B-3001 Leuven, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Steensels
authors.affiliation: Laboratory for Systems Biology, VIB Center for Microbiology, Gaston Geenslaan 1, B-3001 Leuven, Belgium; Laboratory for Genetics and Genomics, KU Leuven, Gaston Geenslaan 1, B-3001 Leuven, Belgium. Electronic address: kevin.verstrepen@biw.vib-kuleuven.be.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Verstrepen
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D064113:CRISPR-Cas Systems / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D044085:Microfluidics