title: Overexpression of miR-519d in lung adenocarcinoma inhibits cell proliferation and invasion via the association of eIF4H.
abstract: Lung cancer is one of the deadliest types of cancer worldwide due to its high mortality rate. Adenocarcinoma constitutes 20%-30% of all lung cancers. In recent years, studies on the mechanisms of lung tumorigenesis and development have in part focused on the microRNAs for their crucial role in the progress of different cancers. As for our study, we demonstrated that miR-519d was differently downregulated and eIF4H was significantly overexpressed in lung adenocarcinoma via the detection of quantitative real-time polymerase chain reaction compared with the adjacent normal tissues. Furthermore, Cell Counting Kit-8 assay, colony formation assay, xenograft tumor experiment, Ki67 immunohistochemistry assay and transwell assay were performed to explain that the upregulated miR-519d could inhibit the proliferation and invasion of A549 and H1299 cells. To further advance our understanding of the mechanisms of miR-519d, we performed the bioinformatics analysis and the luciferase report assay. The results from these procedures revealed eIF4H to be one of the targets of miR-519d. Downregulated eIF4H was analogous to the overexpressed miR-519d obtained from miR-519d agomir and si-eIF4H transfection. In summary, it can be concluded that miR-519d targets eIF4H in lung adenocarcinoma to inhibit cell proliferation and invasion. This mechanism may offer new insights into the tumorigenesis and development of lung adenocarcinoma.
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Chunya
authors.initials: C
authors.lastname: Lu
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: 2 Medical Laboratory Science, Zhengzhou University, Zhengzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hou
authors.affiliation: 3 School of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Guo
authors.affiliation: 3 School of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Heqi
authors.initials: H
authors.lastname: Zhou
authors.affiliation: 4 School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Xiaojingnan
authors.initials: X
authors.lastname: Ma
authors.affiliation: 4 School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D039642:Eukaryotic Initiation Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: C111495:EIF4H protein, human; D039642:Eukaryotic Initiation Factors; C546194:MIRN519 microRNA, human; D035683:MicroRNAs
keywords: eIF4H; invasion; lung adenocarcinoma; miR-519d; proliferation