title: Hybrid collagen sponge and stem cells as a new combined scaffold able to induce the re-organization of endothelial cells into clustered networks.
abstract: The time needed to obtain functional regenerated bone tissue depends on the existence of a reliable vascular support. Current techniques used in clinic, for example after tooth extraction, do not allow regaining or preserving the same bone volume. Our aim is to develop a cellularized active implant of the third generation, equipped with human mesenchymal stem cells to improve the quality of implant vascularization. We seeded a commercialized collagen implant with human mesenchymal stem cells (hMSCs) and then with human umbilical vein endothelial cells (HUVECs). We analyzed the biocompatibility and the behavior of endothelial cells with this implant. We observed a biocompatibility of the active implant, and a re-organization of endothelial cells into clustered networks. This work shows the possibility to develop an implant of the third generation supporting vascularization, improving the medical care of patients.
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.|Hôpitaux Universitaires de Strasbourg (HUS), 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Offner
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Wagner
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Ysia
authors.initials: Y
authors.lastname: Idoux-Gillet
authors.affiliation: Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Gegout
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Arielle
authors.initials: A
authors.lastname: Ferrandon
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Schwinté
authors.affiliation: Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.|Hôpitaux Universitaires de Strasbourg (HUS), 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Musset
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
authors.affiliation: INSERM (French National Institute of Health and Medical Research), 'Osteoarticular and Dental Regenerative Nanomedicine' Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Keller
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018919:Neovascularization, Physiologic; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003094:Collagen
keywords: Collagen implant; endothelial cells; mesenchymal stem cells; vascularization