title: Placental oxidative stress and maternal endothelial function in pregnant women with normotensive fetal growth restriction.
abstract: OBJECTIVE
  The purpose of this study was to investigate the relationship between placental oxidative stress and maternal endothelial function in pregnant women with normotensive fetal growth restriction (FGR).
  
  
  METHODS
  We examined serum concentrations of oxygen free radicals (d-ROMs), maternal angiogenic factor (PlGF), and sFlt-1, placental oxidative DNA damage, and maternal endothelial function in 17 women with early-onset preeclampsia (PE), 18 with late-onset PE, 14 with normotensive FGR, and 21 controls. Flow-mediated vasodilation (FMD) was assessed as a marker of maternal endothelial function. Immunohistochemical analysis was performed to measure the proportion of placental trophoblast cell nuclei staining positive for 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage.
  
  
  RESULTS
  Maternal serum d-ROM, sFlt-1 concentrations, and FMD did not significantly differ between the control and normotensive FGR groups. The proportion of nuclei staining positive for 8-OHdG was significantly higher in the normotensive FGR group relative to the control group.
  
  
  CONCLUSIONS
  Our findings demonstrate that, despite the presence of placental oxidative DNA damage as observed in PE patients, pregnant women with normotensive FGR show no increase in the concentrations of sFlt-1 and d-ROMs, or a decrease in FMD.
authors.affiliation: a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
authors.forename: Atsumi
authors.initials: A
authors.lastname: Yoshida
authors.affiliation: a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Iwasaki
authors.affiliation: a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Kimura
authors.affiliation: a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
authors.forename: Hiroshi
authors.identifier: http://orcid.org/0000-0002-8219-9011
authors.initials: H
authors.lastname: Matsushita
authors.affiliation: a Department of Obstetrics and Gynecology , Aichi Medical University School of Medicine , Nagakute , Aichi , Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Wakatsuki
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004249:DNA Damage; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood* / Q000503:physiopathology; D006801:Humans; D018384:Oxidative Stress; D010920:Placenta / Q000378:metabolism*; D000072483:Placenta Growth Factor / Q000097:blood; D011247:Pregnancy; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood
chemical_list: D000072483:Placenta Growth Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: PlGF; Preeclampsia; endothelial dysfunction; normotensive FGR; oxidative stress; sFlt-1