title: In vivo imaging of neural activity.
abstract: Since the introduction of calcium imaging to monitor neuronal activity with single-cell resolution, optical imaging methods have revolutionized neuroscience by enabling systematic recordings of neuronal circuits in living animals. The plethora of methods for functional neural imaging can be daunting to the nonexpert to navigate. Here we review advanced microscopy techniques for in vivo functional imaging and offer guidelines for which technologies are best suited for particular applications.
authors.affiliation: Department of Biological Sciences, Neurotechnology Center, Columbia University, New York, New York, USA.
authors.forename: Weijian
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Biological Sciences, Neurotechnology Center, Columbia University, New York, New York, USA.
authors.forename: Rafael
authors.identifier: http://orcid.org/0000-0003-0941-3496
authors.initials: R
authors.lastname: Yuste
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D004867:Equipment Design; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D009474:Neurons / Q000502:physiology*
chemical_list: D002118:Calcium