title: Proteomic signature of muscle fibre hyperplasia in response to faba bean intake in grass carp.
abstract: Fish muscle growth is important for the rapidly developing global aquaculture industry, particularly with respect to production and quality. Changes in muscle fibre size are accomplished by altering the balance between protein synthesis and proteolysis. However, our understanding regarding the effects of different protein sources on fish muscle proteins is still limited. Here we report on the proteomic profile of muscle fibre hyperplasia in grass carp fed only with whole faba bean. From the results, a total of 99 significantly changed proteins after muscle hyperplasia increase were identified (p < 0.05, ratio <0.5 or >2). Protein-protein interaction analysis demonstrated the presence of a network containing 56 differentially expressed proteins, and muscle fibre hyperplasia was closely related to a protein-protein network of 12 muscle component proteins. Muscle fibre hyperplasia was also accompanied by decreased abundance in the fatty acid degradation and calcium signalling pathways. In addition, metabolism via the pentose phosphate pathway decreased in grass carp after ingestion of faba bean, leading to haemolysis. These findings could provide a reference for the prevention and treatment of human glucose-6-phosphate dehydrogenase deficiency ("favism").
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Er-Meng
authors.initials: EM
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Hao-Fang
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Zhi-Fei
authors.initials: ZF
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Xingangxi Road, Guangzhou 510275, China.
authors.forename: Hong-Kai
authors.initials: HK
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: De-Guang
authors.initials: DG
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tropical &Subtropical Fishery Resource Application &Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
authors.forename: Wang-Bo
authors.initials: WB
authors.lastname: Gong
mesh_terms: D000821:Animal Feed; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002347:Carps / Q000378:metabolism*; D019587:Dietary Supplements; D005227:Fatty Acids / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism*; D006965:Hyperplasia; D053858:Metabolic Networks and Pathways; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000473:pathology; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D031307:Vicia faba
chemical_list: D005227:Fatty Acids; D029941:Fish Proteins; D020543:Proteome; D002118:Calcium