title: Regulating the gaps between folds on the surface of silk fibroin membranes via LBL deposition for improving their biomedical properties.
abstract: Silk fibroin (SF) has become a promising biomaterial in guided bone regeneration (GBR). In an attempt to modify the size of the gaps on the surface of SF barrier membrane and improve its antibacterial activity, biological and mechanical properties, positively charged Lysozyme (LY)-Collagen Type-I (COL) composites and negatively charged SF were introduced to the negatively charged surface of SF substrates utilizing the electrostatic layer-by-layer (LBL) self-assembly technique. The morphology, chemical structures and element content of the LBL structured membranes were investigated. The results suggested that LY and COL were successfully assembled and the gaps between the folds on the surface of the membranes became smaller gradually with the increase of coated film numbers. Besides, the content of β-sheets of the membranes increased after deposition, which indicated the improvement of their mechanical properties. Moreover, the results of the measurement of immobilized LY and antibacterial assay not only revealed that the enzymatic catalysis and antibacterial activity of the samples enhanced with the increase of coated bilayer numbers but also implied that LBL modified membranes had better antibacterial activity when LY-COL was on the outermost layer. Furthermore, CCK-8 assay certified both SF membrane and LBL structured membranes could facilitate cell growth and proliferation, and the introduction of COL could further promote this ability. Finally, cell attachment and morphology examination provided intuitional evidence that SF membrane and LBL modified membranes have excellent biocompatibility.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Guomin
authors.initials: G
authors.lastname: Wu
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China; Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Tu
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Yingfei
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China; Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: wang.yn@whu.edu.cn.
authors.forename: Yining
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China; Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, 510140, China. Electronic address: xma.cn@hotmail.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001861:Bone Regeneration / Q000502:physiology; D002448:Cell Adhesion; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000737:chemistry* / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D009113:Muramidase / Q000737:chemistry* / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D000072757:Protein Conformation, beta-Strand; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D055672:Static Electricity; D023822:Tissue Engineering / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D024042:Collagen Type I; D005349:Fibroins; D009113:Muramidase
keywords: Antibacterial activity; Cell migration properties; Gaps regulating; LBL deposition; Silk fibroin membrane