title: Chlorogenic acid, anthocyanin and flavan-3-ol biosynthesis in flesh and skin of Andean potato tubers (Solanum tuberosum subsp. andigena).
abstract: Natural variation of Andean potato was used to study the biosynthesis of phenolic compounds. Levels of phenolic compounds and corresponding structural gene transcripts were examined in flesh and skin of tubers. Phenolic acids, mainly chlorogenic acid (CGA), represent the major compounds, followed by anthocyanins and flavan-3-ols. High-anthocyanin varieties have high levels of CGA. Both metabolite and transcript levels were higher in skin than in flesh and showed a good correspondence. Two hydroxycinnamoyl-CoA transferases (HCT/HQT) have been involved in CGA production, of which HCT reflects CGA levels. Catechin was found in pigmented tissues whereas epicatechin was restricted to tuber skin. Transcripts of leucoanthocyanidin reductase (LCR), which generates catechin, could not be detected. Anthocyanidin reductase (ANR) transcripts, the enzyme responsible for epicatechin production, showed similar levels among samples. These data suggest that the biosynthesis of flavan-3-ols in potato tuber would require ANR but not LCR and that an epimerization process is involved.
authors.affiliation: Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: matiasaval@gmail.com.
authors.forename: Matías Ariel
authors.initials: MA
authors.lastname: Valiñas
authors.affiliation: Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: lanteri@mdp.edu.ar.
authors.forename: María Luciana
authors.initials: ML
authors.lastname: Lanteri
authors.affiliation: Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: tenhave.arjen@gmail.com.
authors.forename: Arjen
authors.initials: A
authors.lastname: Ten Have
authors.affiliation: Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina. Electronic address: abandreu@mdp.edu.ar.
authors.forename: Adriana Balbina
authors.initials: AB
authors.lastname: Andreu
mesh_terms: D000872:Anthocyanins / Q000032:analysis / Q000096:biosynthesis*; D001118:Argentina; D002392:Catechin / Q000032:analysis / Q000378:metabolism; D002726:Chlorogenic Acid / Q000032:analysis / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000096:biosynthesis*; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism*; D059808:Polyphenols / Q000032:analysis / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D002726:Chlorogenic Acid; C404987:flavan-3-ol; D002392:Catechin
keywords: Anthocyanidin reductase; Antioxidant; Leucoanthocyanidin reductase; Phenolic compounds metabolism; Solanum tuberosum