title: Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing.
abstract: Gastrointestinal nematodes (GINs) are one of the most economically important parasites of small ruminants and a major animal health concern in many regions of the world. However, the molecular mechanisms of the host response to GIN infections in goat are still little known. In this study, two genetically distinct goat populations, one relatively resistant and the other susceptible to GIN infections, were identified inYichanggoat and then four individuals in each group were chosen to compare mRNA expression profiles using RNA-seq. Field experiment showed lower worm burden, delayed and reduced egg production in the relatively resistant group than the susceptible group. The analysis of RNA-seq showed that 2369 genes, 1407 of which were up-regulated and 962 down-regulated, were significantly (p< 0.001) differentially expressed between these two groups. Functional annotation of the 298 genes more highly expressed in the resistant group yielded a total of 46 significant (p< 0.05) functional annotation clusters including 31 genes (9 in innate immunity, 13 in immunity, and 9 in innate immune response) related to immune biosynthetic process as well as transforming growth factor (TGF)-β, mitogen-activated protein kinase (MAPK), and cell adhesion molecules (CAMs) pathways. Our findings provide insights that are immediately relevant for the improvement of host resistance to GIN infections and which will make it possible to know the mechanisms underlying the resistance of goats to GIN infections.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. aab76_blri@yahoo.com.|Senior Scientific Officer, Bangladesh Livestock Research Institute, Savar, Dhaka-1341, Bangladesh. aab76_blri@yahoo.com.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Bhuiyan
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. jingjinli@webmail.hzau.edu.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. wuzhenyang0724@gmail.com.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. nipanmato@gmail.com.
authors.forename: Pan
authors.initials: P
authors.lastname: Ni
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. quueenyk10@yahoo.com.
authors.forename: Adeyinka Abiola
authors.initials: AA
authors.lastname: Adetula
authors.affiliation: College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. info_blri@yahoo.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. aabso_blri@yahoo.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Agricultural Animal Husbandry, Tibet University, Linzhi 850012, China. s_azmal@yahoo.com.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. aab_bau@yahoo.com.
authors.forename: Anjuman Ara
authors.initials: AA
authors.lastname: Bhuyan
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. shzhao@mail.hzau.edu.cn.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. duxiaoyong@mail.hzau.edu.cn.|College of Informatics, Huazhong Agricultural University, Wuhan 430070, China. duxiaoyong@mail.hzau.edu.cn.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Du
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D060467:Disease Resistance / Q000235:genetics*; D005767:Gastrointestinal Diseases / Q000235:genetics* / Q000469:parasitology / Q000662:veterinary; D020869:Gene Expression Profiling / Q000379:methods; D063990:Gene Ontology; D015511:Goat Diseases / Q000235:genetics* / Q000469:parasitology; D006041:Goats; D006188:Haemonchiasis / Q000235:genetics* / Q000469:parasitology / Q000662:veterinary; D006189:Haemonchus / Q000254:growth & development* / Q000502:physiology; D006790:Host-Parasite Interactions; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics
keywords: RNA-seq; gastrointestinal nematodes; genetic resistant; goat; transcriptome