title: In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina.
abstract: Neonatal in vivo electroporations and retinal explant electroporations have been widely employed in understanding the effects of loss or gain of function of protein-coding genes in retinal development. Here, we describe a rapid and efficient delivery of morpholinos to add another tool to perturb gene expression during mouse retinal development.
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Road, Storrs, CT, 06269, USA.
authors.forename: Devi Krishna Priya
authors.initials: DK
authors.lastname: Karunakaran
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Road, Storrs, CT, 06269, USA. Rahul.kanadia@uconn.edu.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kanadia
mesh_terms: D000818:Animals; D018274:Electroporation / Q000379:methods; D015870:Gene Expression; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D051379:Mice; D008856:Microscopy, Fluorescence; D060172:Morpholinos / Q000008:administration & dosage / Q000235:genetics*; D012160:Retina / Q000378:metabolism*
chemical_list: D060172:Morpholinos
keywords: Explant electroporation; Green fluorescent protein (GFP); In vivo electroporations; Retinal development; Subretinal space; u12 morpholinos