title: A novel voltammetric sensor for nevirapine, based on modified graphite electrode by MWCNs/poly(methylene blue)/gold nanoparticle.
abstract: In the present study, a graphite electrode (GE) modified by conductive film (containing functionalized multi-walled carbon nanotubes (f-MWCNTs), poly methylene blue p(MB) and gold nanoparticles (AuNPs)) was introduced for determination of nevirapine (NVP) as an anti-HIV drug by applying the differential pulse anodic stripping voltammetry (DPASV) technique. Modification of the electrode was investigated by scanning electron microscopy (SEM) and impedance electrochemical spectroscopy (EIS). All electrochemical effective parameters on detection of NVP were optimized and the oxidation peak current of drug was used for its monitoring. The obtained results confirmed that the oxidation peak currents increased linearly by increasing in NVP concentrations in the range of 0.1-50 μM and a detection limit of 53 nM was achieved. The proposed sensor (AuNPs/p(MB)/f-MWCNTs/GE) was successfully applied for the determination of NVP in blood serum and pharmaceutical samples. It revealed the excellent stability, repeatability and reproducibility as well.
authors.affiliation: Department of Chemistry, College of Science, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran; Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mbgholivand@yahoo.com.
authors.forename: Mohammad Bagher
authors.initials: MB
authors.lastname: Gholivand
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Elahe
authors.initials: E
authors.lastname: Ahmadi
authors.affiliation: Department of Biotechnology-Chemical Engineering, College of Science, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
authors.forename: Mozhdeh
authors.initials: M
authors.lastname: Haseli
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008751:Methylene Blue / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D019829:Nevirapine / Q000097:blood*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results
chemical_list: D019380:Anti-HIV Agents; D037742:Nanotubes, Carbon; D006046:Gold; D006108:Graphite; D019829:Nevirapine; D008751:Methylene Blue
keywords: Electro-polymerization; Gold nanoparticles; Methylene blue; Nevirapine; Stripping voltammetry