title: AtPME3, a ubiquitous cell wall pectin methylesterase of Arabidopsis thaliana, alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings.
abstract: AtPME3 (At3g14310) is a ubiquitous cell wall pectin methylesterase. Atpme3-1 loss-of-function mutants exhibited distinct phenotypes from the wild type (WT), and were characterized by earlier germination and reduction of root hair production. These phenotypical traits were correlated with the accumulation of a 21.5-kDa protein in the different organs of 4-day-old Atpme3-1 seedlings grown in the dark, as well as in 6-week-old mutant plants. Microarray analysis showed significant down-regulation of the genes encoding several pectin-degrading enzymes and enzymes involved in lipid and protein metabolism in the hypocotyl of 4-day-old dark grown mutant seedlings. Accordingly, there was a decrease in proteolytic activity of the mutant as compared with the WT. Among the genes specifying seed storage proteins, two encoding CRUCIFERINS were up-regulated. Additional analysis by RT-qPCR showed an overexpression of four CRUCIFERIN genes in the mutant Atpme3-1, in which precursors of the α- and β-subunits of CRUCIFERIN accumulated. Together, these results provide evidence for a link between AtPME3, present in the cell wall, and CRUCIFERIN metabolism that occurs in vacuoles.
authors.affiliation: BIOPI Biologie des Plantes et Innovation EA3900, Université de Picardie Jules Verne, 33 Rue Saint Leu, 80039 Amiens Cedex, France.|CRRBM, Bâtiment Serres Transfert, Université de Picardie Jules Verne, 33 Rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Guénin
authors.affiliation: Université de Rouen Normandie, CNRS, Laboratoire PBS, 76000 Rouen, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
authors.affiliation: Université de Rouen Normandie, Laboratoire Glyco-MEV, 76000 Rouen, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Paynel
authors.affiliation: Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V6A 1S6, Canada.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Müller
authors.affiliation: CRRBM, Bâtiment Serres Transfert, Université de Picardie Jules Verne, 33 Rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Mongelard
authors.affiliation: Université de Rouen Normandie, Laboratoire Glyco-MEV, 76000 Rouen, France.
authors.forename: Azeddine
authors.initials: A
authors.lastname: Driouich
authors.affiliation: Université de Rouen Normandie, Laboratoire Glyco-MEV, 76000 Rouen, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.affiliation: Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V6A 1S6, Canada.
authors.forename: Allison R
authors.initials: AR
authors.lastname: Kermode
authors.affiliation: Université de Rouen Normandie, Laboratoire Glyco-MEV, 76000 Rouen, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lehner
authors.affiliation: Université de Rouen Normandie, Laboratoire Glyco-MEV, 76000 Rouen, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Mollet
authors.affiliation: BIOPI Biologie des Plantes et Innovation EA3900, Université de Picardie Jules Verne, 33 Rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pelloux
authors.affiliation: CRRBM, Bâtiment Serres Transfert, Université de Picardie Jules Verne, 33 Rue Saint Leu, 80039 Amiens Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutierrez
authors.affiliation: Université de Rouen Normandie, Laboratoire Glyco-MEV, 76000 Rouen, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Mareck
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000502:physiology*; D002265:Carboxylic Ester Hydrolases / Q000502:physiology*; D002473:Cell Wall / Q000201:enzymology; D017343:Genes, Plant / Q000502:physiology; D018525:Germination; D020411:Oligonucleotide Array Sequence Analysis; D055314:Seed Storage Proteins / Q000378:metabolism*; D036226:Seedlings / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D055314:Seed Storage Proteins; D002265:Carboxylic Ester Hydrolases; C568893:pectin methylesterase 3, Arabidopsis
keywords: Arabidopsis; AtPME3; CRUCIFERIN; etiolated hypocotyl; pectin methylesterase; seed germination; transcriptomic analyses.