title: Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos.
abstract: Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.
authors.affiliation: Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA.
authors.forename: Qiuming
authors.initials: Q
authors.lastname: Gong
authors.affiliation: Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA. zhouzh@ohsu.edu.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D017398:Alternative Splicing; D000072237:ERG1 Potassium Channel / Q000235:genetics; D051638:Ether-A-Go-Go Potassium Channels / Q000235:genetics; D005786:Gene Expression Regulation; D023061:Gene Order; D057809:HEK293 Cells; D006801:Humans; D008133:Long QT Syndrome / Q000235:genetics; D060172:Morpholinos / Q000235:genetics*; D011061:Poly A; D026723:Polyadenylation; D020033:Protein Isoforms / Q000235:genetics; D014162:Transfection
chemical_list: D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; C000606913:KCNH2 protein, human; D060172:Morpholinos; D020033:Protein Isoforms; D011061:Poly A
keywords: Alternative polyadenylation; KCNH2; Long QT syndrome; Morpholino; Potassium channel; hERG