title: EphB3-targeted regulation of miR-149 in the migration and invasion of human colonic carcinoma HCT116 and SW620 cells.
abstract: microRNAs play key roles during various crucial cell processes such as proliferation, migration, invasion and apoptosis. Also, microRNAs have been shown to possess oncogenic and tumor-suppressive functions in human cancers. Here, we describe the regulation and function of miR-149 in colorectal cancer cell lines. miR-149 expression patterns were detected in human colorectal cell lines and tissue samples, and then focused on its role in regulation of cell growth, migration, invasion, and its target gene identification. Furthermore, the function of the target gene of miR-149 was analyzed in vitro and in vivo. miR-149 expression was downregulated in human colorectal cancer HCT116 and SW620 cell lines compared to the normal colon epithelial NCM460 cell line using quantitative real-time polymerase chain reaction methods. Further studies indicated that introduction of miR-149 was able to suppress cell migration and invasion. Then, EphB3 was identified as a direct target gene of miR-149 in colorectal cancer cells. Moreover, experiments in vitro showed that knockdown expression of EphB3 could suppress cell proliferation and invasion, and ectopic expression of EphB3 restored the phenotypes of CRC cell lines transfected with miR149. In addition, silencing of EphB3 significantly affected cycle progression distribution and increased apoptosis in CRC cell lines. Finally, in vivo results demonstrated that knockdown of EphB3 by siRNA inhibited tumor growth. In conclusion,the important role of miR-149 in colorectal cancer progression suggesting that miR-149 may serve as a therapeutic target for colorectal cancer treatment.
authors.affiliation: Department of Medical Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Light Industry Engineering, Guizhou Institute of Technology, Guiyang, Guizhou, China.
authors.forename: Xiaozhu
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Light Industry Engineering, Guizhou Institute of Technology, Guiyang, Guizhou, China.
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shangdong Medicine Technician College, Tai'an, Shandong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangzhou Youdi Biotechnology Company, Guangzhou, Guangdong, China.
authors.forename: Huankun
authors.initials: H
authors.lastname: Liang
authors.affiliation: Guangzhou Youdi Biotechnology Company, Guangzhou, Guangdong, China.
authors.forename: Kangyan
authors.initials: K
authors.lastname: Li
authors.affiliation: Guangzhou Youdi Biotechnology Company, Guangzhou, Guangdong, China.
authors.forename: Laiqing
authors.initials: L
authors.lastname: Li
authors.affiliation: Guangzhou Youdi Biotechnology Company, Guangzhou, Guangdong, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Chen
authors.affiliation: Cancer Center of The 88 Hospital of People's Liberation Army, Tai'an, Shandong, China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Sun
authors.affiliation: Shangdong Medicine Technician College, Tai'an, Shandong, China.
authors.forename: Shoulei
authors.initials: S
authors.lastname: Ren
authors.affiliation: Shangdong Medicine Technician College, Tai'an, Shandong, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Cancer Center of The 88 Hospital of People's Liberation Army, Tai'an, Shandong, China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009368:Neoplasm Transplantation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D036223:Receptor, EphB3 / Q000235:genetics / Q000378:metabolism*; D014183:Transplantation, Heterologous
chemical_list: C551557:MIRN149 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D036223:Receptor, EphB3
keywords: EphB3; human colorectal cell; invasion; miR-149; migration