title: Lectin-functionalized mesoporous silica nanoparticles for endoscopic detection of premalignant colonic lesions.
abstract: Colorectal cancer (CRC) is one of the leading causes of cancer-deaths worldwide. Methods for the early in situ detection of colorectal adenomatous polyps and their precursors - prior to their malignancy transformation into CRC - are urgently needed. Unfortunately at present, the primary diagnostic method, colonoscopy, can only detect polyps and carcinomas by shape/morphology; with sessile polyps more likely to go unnoticed than polypoid lesions. Here we describe our development of polyp-targeting, fluorescently-labeled mesoporous silica nanoparticles (MSNs) that serve as targeted endoscopic contrast agents for the early detection of colorectal polyps and cancer. In vitro cell studies, ex vivo histopathological analysis, and in vivo colonoscopy and endoscopy of murine colorectal cancer models, demonstrate significant binding specificity of our nanoconstructs to pathological lesions via targeting aberrant α-L-fucose expression. Our findings strongly suggest that lectin-functionalized fluorescent MSNs could serve as a promising endoscopic contrast agent for in situ diagnostic imaging of premalignant colonic lesions.
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes Zhunan, Miaoli, Taiwan; Department of Chemistry, National Taiwan University Taipei, Taiwan; Department of Radiology, The University of Chicago, Chicago, IL, USA; Institute of New Drug Development, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.
authors.forename: Nai-Tzu
authors.initials: NT
authors.lastname: Chen
authors.affiliation: Department of Radiology, The University of Chicago, Chicago, IL, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Souris
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes Zhunan, Miaoli, Taiwan; Department of Radiology, The University of Chicago, Chicago, IL, USA.
authors.forename: Shih-Hsun
authors.initials: SH
authors.lastname: Cheng
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes Zhunan, Miaoli, Taiwan.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Chu
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes Zhunan, Miaoli, Taiwan.
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, IL, USA.
authors.forename: Vani
authors.initials: V
authors.lastname: Konda
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, IL, USA.
authors.forename: Urszula
authors.initials: U
authors.lastname: Dougherty
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, IL, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Bissonnette
authors.affiliation: Department of Chemistry, National Taiwan University Taipei, Taiwan.
authors.forename: Chung-Yuan
authors.initials: CY
authors.lastname: Mou
authors.affiliation: Department of Radiology, The University of Chicago, Chicago, IL, USA.
authors.forename: Chin-Tu
authors.initials: CT
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes Zhunan, Miaoli, Taiwan. Electronic address: lwlo@nhri.org.tw.
authors.forename: Leu-Wei
authors.initials: LW
authors.lastname: Lo
mesh_terms: D000818:Animals; D003106:Colon / Q000473:pathology; D003111:Colonic Polyps / Q000175:diagnosis*; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000175:diagnosis*; D004724:Endoscopy / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D053758:Nanoparticles / Q000737:chemistry*; D011230:Precancerous Conditions / Q000175:diagnosis*; D012822:Silicon Dioxide / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D037102:Lectins; D012822:Silicon Dioxide
keywords: Colorectal tumors; Endoscopic; Mesoporous silica nanoparticle; Ulex europaeus Agglutinin 1 (UEA1)