title: Rapid and Reliable Genotyping of HLA-B*57:01 in Four Chinese Populations Using a Single-Tube Duplex Real-Time Polymerase Chain Reaction Assay.
abstract: HLA-B*57:01 is strongly associated with severe adverse drug reaction induced by the anti-HIV drug abacavir (ABC) and antibiotic flucloxacillin. This study was dedicated to establishing a new method for HLA-B*57:01 genotyping and investigating the HLA-B*57:01 distribution pattern in four Chinese populations. A single-tube duplex real-time polymerase chain reaction (PCR) system was established by combining the amplification refractory mutation system and TaqMan probe. The reliability of this assay was validated by comparing the genotyping results with those by sequence-based typing. With this assay, the distribution of HLA-B*57:01 in 354 blood samples from four ethnic groups, namely, Han, Tibetan, Uighur, and Buyei, was determined. A 100% concordance was observed between the results of real-time PCR and sequence-based typing in 50 Uighur samples. As low as 0.016 ng DNA that carried HLA-B*57:01 could be detected with this assay. HLA-B*57:01 carriers identified in 100 Northern Han Chinese, 104 Buyeis, 100 Tibetans, and 50 Uighurs were 0, 1 (0.96%), 3 (3%), and 6 (12%), respectively. The carrier rate of HLA-B*57:01 in Uighur was significantly higher than those in Northern Han (p = .001) and Buyei (p = .005). The newly established real-time PCR assay provides a rapid and reliable tool for HLA-B*57:01 allele screening before the prescription of ABC and flucloxacillin in clinical practice.
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Xing
authors.initials: X
authors.lastname: Kang
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University , Xi'an, China .
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wang
mesh_terms: D044466:Asian People; D015331:Cohort Studies; D015224:Dideoxynucleosides / Q000627:therapeutic use; D064420:Drug-Related Side Effects and Adverse Reactions / Q000517:prevention & control*; D005820:Genetic Testing / Q000379:methods; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D015658:HIV Infections / Q000188:drug therapy; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D000099227:Abacavir
chemical_list: D015224:Dideoxynucleosides; D015235:HLA-B Antigens; D000099227:Abacavir; C103707:HLA-B57 antigen
keywords: HLA-B*57:01; abacavir; hypersensitivity reaction; real-time PCR