title: Lipid-coated iron oxide nanoparticles for dual-modal imaging of hepatocellular carcinoma.
abstract: The development of noninvasive imaging techniques for the accurate diagnosis of progressive hepatocellular carcinoma (HCC) is of great clinical significance and has always been desired. Herein, a hepatocellular carcinoma cell-targeting fluorescent magnetic nanoparticle (NP) was obtained by conjugating near-infrared fluorescence to the surface of Fe3O4(NIRF-Fe3O4) NPs, followed by coating the lipids consisting of tumoral hepatocytes-targeting polymer (Gal-P123). This magnetic NP (GPC@NIRF-Fe3O4) with superparamagnetic behavior showed high stability and safety in physiological conditions. In addition, GPC@NIRF-Fe3O4achieved more specific uptake of human liver cancer cells than free Fe3O4NPs. Importantly, with superpara-magnetic iron oxide and strong NIR absorbance, GPC@NIRF-Fe3O4NPs demonstrate prominent tumor-contrasted imaging performance both on fluorescent and T2-weighted magnetic resonance (MR) imaging modalities in a living body. The relative MR signal enhancement of GPC@NIRF-Fe3O4NPs achieved 5.4-fold improvement compared with NIR-Fe3O4NPs. Therefore, GPC@ NIRF-Fe3O4NPs may be potentially used as a candidate for dual-modal imaging of tumors with information covalidated and directly compared by combining fluorescence and MR imaging.
authors.affiliation: Department of Pharmaceutics, China Pharmaceutical University, Nanjing, People's Republic of China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People's Republic of China; School of Pharmacy, Xinxiang Medical University, Xinxiang, People's Republic of China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Liang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors.forename: Yunqiu
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Pharmaceutics, China Pharmaceutical University, Nanjing, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gan
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging* / Q000473:pathology; D005290:Ferric Compounds / Q000737:chemistry; D005453:Fluorescence; D022781:Hepatocytes / Q000473:pathology; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008113:Liver Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D023041:Xenograft Model Antitumor Assays
chemical_list: D005290:Ferric Compounds; D008055:Lipids; D058185:Magnetite Nanoparticles; C000499:ferric oxide
keywords: dual-imaging; hepatocellular carcinoma; magnetic resonance imaging; tumor-targeting