title: Metagenome sequencing and 98 microbial genomes from Juan de Fuca Ridge flank subsurface fluids.
abstract: The global deep subsurface biosphere is one of the largest reservoirs for microbial life on our planet. This study takes advantage of new sampling technologies and couples them with improvements to DNA sequencing and associated informatics tools to reconstruct the genomes of uncultivated Bacteria and Archaea from fluids collected deep within the Juan de Fuca Ridge subseafloor. Here, we generated two metagenomes from borehole observatories located 311 meters apart and, using binning tools, retrieved 98 genomes from metagenomes (GFMs). Of the GFMs, 31 were estimated to be >90% complete, while an additional 17 were >70% complete. Phylogenomic analysis revealed 53 bacterial and 45 archaeal GFMs, of which nearly all were distantly related to known cultivated isolates. In the GFMs, abundant Bacteria included Chloroflexi, Nitrospirae, Acetothermia (OP1), EM3, Aminicenantes (OP8), Gammaproteobacteria, and Deltaproteobacteria, while abundant Archaea included Archaeoglobi, Bathyarchaeota (MCG), and Marine Benthic Group E (MBG-E). These data are the first GFMs reconstructed from the deep basaltic subseafloor biosphere, and provide a dataset available for further interrogation.
authors.affiliation: Center for Dark Energy Biosphere Investigations, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Sean P
authors.identifier: 0000-0001-9265-8341
authors.initials: SP
authors.lastname: Jungbluth
authors.affiliation: Center for Dark Energy Biosphere Investigations, University of Southern California, Los Angeles, California 90089, USA.|Department of Earth Sciences, University of Southern California, Los Angeles, California 90089, USA.|Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Jan P
authors.initials: JP
authors.lastname: Amend
authors.affiliation: Hawaii Institute of Marine Biology, SOEST, University of Hawaii, Kaneohe, Hawaii 96744, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Rappé
mesh_terms: D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D064349:Genome, Microbial; D054892:Metagenome; D009656:North America; D009792:Oceans and Seas; D012623:Seawater