title: The Cell-Surface N-Glycome of Human Embryonic Stem Cells and Differentiated Hepatic Cells thereof.
abstract: Human embryonic stem cells (hESCs) are pluripotent stem cells that offer a wide range of applications in regenerative medicine. In addition, they have been proposed as an appropriate alternative source of hepatocytes. In this work, hESCs were differentiated into definitive endodermal cells (DECs), followed by maturation into hepatocyte-like cells (HLCs). Their cell-surface N-glycome was profiled and also compared with that of primary human hepatocytes (PHHs). Undifferentiated hESCs contained large amounts of high-mannose N-glycans. In contrast, complex-type N-glycans such as asialylated or monosialylated biantennary and triantennary N-glycans were dominant in HLCs, and fully galactosylated structures were significantly more abundant than in undifferentiated hESCs. The cell-surface N-glycosylation of PHHs was more biologically processed than that of HLCs, with bisialylated biantennary and trisialylated triantennary structures predominant. This is the first report of the cell surface N-glycome of PHHs and of HLCs being directly generated from hESCs without embryoid body formation.
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Freie Universität Berlin, Department of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195, Berlin, Germany.
authors.forename: Houda
authors.initials: H
authors.lastname: Montacir
authors.affiliation: Bioreactor Group, Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Freyer
authors.affiliation: Bioreactor Group, Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Fanny
authors.initials: F
authors.lastname: Knöspel
authors.affiliation: Bioreactor Group, Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Urbaniak
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.|Freie Universität Berlin, Department of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195, Berlin, Germany.
authors.forename: Tereza
authors.initials: T
authors.lastname: Dedova
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Berger
authors.affiliation: Department of General, Visceral and Transplantation Surgery, Charité-Universitätsmedizin Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, 13353, Berlin, Germany.|Department of Hepatobiliary Surgery and Visceral Transplantation, University of Leipzig, Liebigstrasse 20, 04103, Leipzig, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Damm
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Tauber
authors.affiliation: Bioreactor Group, Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Zeilinger
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
authors.forename: Véronique
authors.initials: V
authors.lastname: Blanchard
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002454:Cell Differentiation; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism; D015870:Gene Expression; D054794:Glycomics; D006031:Glycosylation; D022781:Hepatocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D061251:Primary Cell Culture; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011134:Polysaccharides; D014157:Transcription Factors; D003577:Cytochrome P-450 Enzyme System; D008358:Mannose
keywords: N-glycans; carbohydrates; glycosylation; hepatocytes; human embryonic stem cells