title: Co-microencapsulation of BMSCs and mouse pancreatic β cells for improving the efficacy of type I diabetes therapy.
abstract: BACKGROUND
  To overcome the shortcomings of pancreas transplantation and insulin injection treatment for type I diabetes, biocompatible materials were used to prepare alginate-chitosan-alginate microcapsules that co-encapsulated bone marrow mesenchymal stem cells and mouse pancreatic β cells to treat diabetic mice.
  
  
  METHODS
  Blank alginate-chitosan-alginate (ACA) microcapsules and co-microencapsulated cells were prepared using a high-voltage electrostatic method and then characterized using an inverted microscope. Cell viability was evaluated using AO/EB staining. ELISA kit was used to detect insulin secretion. Peri-orbital blood samples were obtained from the mice for blood glucose determination every week for one month.
  
  
  RESULTS
  After 28 days of in vitro culture, the secretion of insulin following co-microencapsulation was higher than that observed for microencapsulated beta-TC-6 cells alone. On the 28th day after transplantation, the blood glucose level was 6.86 mmol/L in the microencapsulated beta-TC-6 group. On the 14th day, the blood glucose level was 6.80 mmol/L in the co-microencapsulated BMSC/beta-TC-6 group, which was close to the normal blood glucose level of healthy mice. These results indicated that the efficacy in reducing blood glucose was better in the co-microencapsulated BMSC/beta-TC-6 group.
  
  
  CONCLUSIONS
  This primary study indicated that combining microencapsulation technology and co-culture of stem cells and somatic cells shows promise for the treatment of type I diabetes mellitus.
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen - China.|Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen - China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Long
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen - China.|Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen - China.|Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen - China.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen - China.|Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen - China.|Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen - China.
authors.forename: Shibin
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen - China.
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen - China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
mesh_terms: D000464:Alginates; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002214:Capsules; D048271:Chitosan; D018920:Coculture Techniques; D003921:Diabetes Mellitus, Experimental; D003922:Diabetes Mellitus, Type 1 / Q000628:therapy*; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000166:cytology*; D016381:Islets of Langerhans Transplantation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice
chemical_list: D000464:Alginates; D001786:Blood Glucose; D002214:Capsules; D007328:Insulin; D048271:Chitosan