title: Multicenter evaluation of an enzymatic method for glycated albumin.
abstract: BACKGROUND
  The use of glycated albumin (GA) has been proposed as an additional glycemic control marker particularly useful in intermediate-term monitoring and in situation when HbA1ctest is not reliable.
  
  
  METHODS
  We have performed the first multicenter evaluation of the analytical performance of the enzymatic method quantILab Glycated Albumin assay implemented on the most widely used clinical chemistry analyzers (i.e. Abbott Architect C8000, Beckman Coulter AU 480 and 680, Roche Cobas C6000, Siemens ADVIA 2400 and 2400 XPT).
  
  
  RESULTS
  The repeatability of the GA measurement (expressed as CV, %) implemented in the participating centers ranged between 0.9% and 1.2%. The within-laboratory CVs ranged between 1.2% and 1.6%. A good alignment between laboratories was found, with correlation coefficients from 0.996 to 0.998. Linearity was confirmed in the range from 7.6 to 84.7%.
  
  
  CONCLUSIONS
  The new enzymatic method for glycated albumin evaluated by our investigation is suitable for clinical use.
authors.affiliation: Dip. di Fisiopatologia Medico-Chirurgica e dei Trapianti and Centro per la Riferibilità Metrologica in Medicina di Laboratorio (CIRME), Università degli Studi di Milano, Milano, Italy.
authors.forename: Renata
authors.initials: R
authors.lastname: Paleari
authors.affiliation: Laboratorio Analisi Chimico Cliniche, ASST Spedali Civili, Brescia, Italy.
authors.forename: Graziella
authors.initials: G
authors.lastname: Bonetti
authors.affiliation: Polo Scienze delle Immagini, di Laboratorio ed Infettivologiche. Fondazione Policlinico Universitario A. Gemelli, Università Cattolica Sacro Cuore, Roma, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Callà
authors.affiliation: Laboratorio Analisi, Ospedale S. Bortolo, Vicenza, Italy.
authors.forename: Mariarosa
authors.initials: M
authors.lastname: Carta
authors.affiliation: Central Laboratory, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milan, Italy.
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Ceriotti
authors.affiliation: Instrumentation Laboratory - A Werfen Company, R&D Department, Milano, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Di Gaetano
authors.affiliation: Laboratorio Analisi di Urbino, Azienda Sanitaria Unica Regione Marche Area Vasta 1, Fano, PU, Italy.
authors.forename: Marilisa
authors.initials: M
authors.lastname: Ferri
authors.affiliation: Laboratorio di Standardizzazione, Servizio Medicina di Laboratorio, Ospedale S. Raffaele, Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Guerra
authors.affiliation: Laboratorio Analisi, Ospedale Padre Pio di Bracciano, Roma, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Lavalle
authors.affiliation: UOC Laboratorio Analisi, Azienda Ospedaliera Universitaria Integrata Verona, Italy.
authors.forename: Claudia Lo
authors.initials: CL
authors.lastname: Cascio
authors.affiliation: U.O.C. Patologia Clinica (HB 5 Regionale) P.O. San Filippo Neri ASL ROMA A, Italy.
authors.forename: Francesca Gabriela
authors.initials: FG
authors.lastname: Martino
authors.affiliation: Sezione di Biochimica Clinica, Dip. di Neuroscienze, Biomedicina e Movimento, Università degli Studi di Verona, Verona, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Montagnana
authors.affiliation: Patologia Clinica Azienda Ospedaliera Ospedali Riuniti Marche Nord Pesaro, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Moretti
authors.affiliation: Instrumentation Laboratory - A Werfen Company, R&D Department, Milano, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Santini
authors.affiliation: Polo Scienze delle Immagini, di Laboratorio ed Infettivologiche. Fondazione Policlinico Universitario A. Gemelli, Università Cattolica Sacro Cuore, Roma, Italy.
authors.forename: Donata
authors.initials: D
authors.lastname: Scribano
authors.affiliation: Modelli di Integrazione Multidisciplinare in Patologia Clinica, INRCA-IRCCS, Ancona, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Testa
authors.affiliation: Laboratorio di Analisi Chimico-Clinica, Azienda Ospedaliera Pugliese Ciaccio, Catanzaro, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Vero
authors.affiliation: Dip. di Fisiopatologia Medico-Chirurgica e dei Trapianti and Centro per la Riferibilità Metrologica in Medicina di Laboratorio (CIRME), Università degli Studi di Milano, Milano, Italy. Electronic address: andrea.mosca@unimi.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mosca
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D017127:Glycation End Products, Advanced; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis* / Q000378:metabolism; D000093423:Glycated Serum Albumin
chemical_list: D004798:Enzymes; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D000093423:Glycated Serum Albumin
keywords: Diabetes; Glycated albumin; Multicenter evaluation