title: A simple stripping voltammetric method for the determination of a new anticancer prodrug in serum.
abstract: The determination of ethyl [4-oxo-8-(3-chlorophenyl)-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazin-3-yl]acetate (ETTA), a new anticancer prodrug, using adsorptive stripping voltammetry (AdSV) was described for the first time. This method is based on adsorptive/reductive behaviour of ETTA at an in situ plated bismuth film electrode (BiFE) as a sensor. A number of experimental variables (e.g., a composition and pH of the supporting electrolyte, the conditions of bismuth film deposition, an accumulation potential and time, the scan rate, etc.) were thoroughly studied in order to achieve a high sensitivity. Experimental results under optimized conditions revealed an excellent linear correlation between the monitored voltammetric peak current and the ETTA concentration in the range of 2-50μgL-1following an accumulation time of 300s. The limit of detection (LOD) for ETTA following 300s of an accumulation time was 0.4μgL-1. The proposed facile, sensitive and inexpensive method was successfully applied to the determination of ETTA in serum. The investigated prodrug was extracted from serum using SPE method.
authors.affiliation: Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, 13 Akademicka, 20-950 Lublin, Poland. Electronic address: anna.stepniowska@up.lublin.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Stępniowska
authors.affiliation: Chair and Department of Medical Chemistry, Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sztanke
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Tuzimski
authors.affiliation: Faculty of Chemistry, Maria Curie-Skłodowska University, 3 Maria Curie-Skłodowska Sq., 20-031 Lublin, Poland.
authors.forename: Mieczysław
authors.initials: M
authors.lastname: Korolczuk
authors.affiliation: Laboratory of Bioorganic Synthesis and Analysis, Chair and Department of Medical Chemistry, Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Sztanke
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D001729:Bismuth / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009369:Neoplasms / Q000097:blood* / Q000188:drug therapy; D011355:Prodrugs / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011355:Prodrugs; D001729:Bismuth
keywords: Adsorptive stripping voltammetry; Anticancer prodrug; Bismuth film electrode; ETTA