title: Isolation of Nuclear RNA-Associated Protein Complexes.
abstract: Noncoding RNAs, being at the center stage of the organismal development and homeostasis, warrant a detailed analysis to utilize their full therapeutic potential. They form complexes with various proteins that enable the noncoding RNAs to acquire specific cellular functions such as the transcriptional outcomes that are controlled in a spatio-temporal manner. In this protocol, we describe a method to isolate such known (and unknown) protein complexes bound to a nuclear noncoding RNA.
authors.affiliation: Howard Hughes Medical Institute, Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
authors.forename: Ranveer Singh
authors.initials: RS
authors.lastname: Jayani
authors.affiliation: Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
authors.forename: Amanjot
authors.initials: A
authors.lastname: Singh
authors.affiliation: Howard Hughes Medical Institute, Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA. dnotani@ncbs.res.in.|Natoinal Center for Biological Sciences, Bangalore, Karnataka, 560065, India. dnotani@ncbs.res.in.
authors.forename: Dimple
authors.initials: D
authors.lastname: Notani
mesh_terms: D003001:Cloning, Molecular; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D015138:RNA, Nuclear / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000302:isolation & purification* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D062085:RNA, Long Noncoding; D015138:RNA, Nuclear; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins
keywords: Biotin-labeling; In vitro transcription; Long noncoding RNA (LncRNA); Mass-spectrometry; RNA-pull-down