title: Cellulase production through solid-state tray fermentation, and its use for bioethanol from sorghum stover.
abstract: The production of cellulase by Trichoderma reesei RUT C-30 under solid-state fermentation (SSF) on wheat bran and cellulose was optimized employing a two stage statistical design of experiments. Optimization of process parameters resulted in a 3.2-fold increase in CMCase production to 959.53IU/gDS. The process was evaluated at pilot scale in tray fermenters and yielded 457IU/gDS using the lab conditions and indicating possibility for further improvement. The cellulase could effectively hydrolyze alkali pretreated sorghum stover and addition of Aspergillus niger β-glucosidase improved the hydrolytic efficiency 174%, indicating the potential to use this blend for effective saccharification of sorghum stover biomass. The enzymatic hydrolysate of sorghum stover was fermented to ethanol with ∼80% efficiency.
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), CSIR-NIIST Campus, Thiruvananthapuram, India; Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Ayman Salih Omer
authors.initials: ASO
authors.lastname: Idris
authors.affiliation: Centre for Innovative and Applied Bioprocessing, Mohali 160 071, Punjab, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Indian Institute of Millets Research, Hyderabad, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Rao
authors.affiliation: Academy of Scientific and Innovative Research (AcSIR), CSIR-NIIST Campus, Thiruvananthapuram, India; Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India. Electronic address: rajeevs@niist.res.in.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Sukumaran
mesh_terms: D002480:Cellulase; D002482:Cellulose; D005285:Fermentation; D006868:Hydrolysis; D045868:Sorghum; D014242:Trichoderma; D001617:beta-Glucosidase
chemical_list: D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Beta glucosidase; Bioethanol; Cellulase; Solid-state fermentation; Sorghum; Trichoderma