title: Interactions between iodinated contrast media and tissue plasminogen activator: In vitro comparison study.
abstract: OBJECTIVE
  Iodinated contrast media (Xenetix®, Ultravist®, Omnipaque®, Visipaque® and Iomeron®) used for computed tomography (CT) may decrease fibrinolysis by recombinant tissue plasminogen activator (rt-PA). We hypothesized that receiving iodinated contrast media before rt-PA may impair thrombolysis as measured by a new model system.
  
  
  METHODS
  Whole blood from Wistar Kyoto rats (n = 10) was obtained and allowed to form blood clots. Thrombolysis was performed by placing individually the prepared clots into 15 mL tubes and adding 5 mL saline buffer, 100μg rt-PA and a different contrast media; adjusting the quantity of iodine to either 30 mg or 60 mg. The thrombolytic efficacy was quantified by measuring the optical density (OD415) of the supernatant at different time points, namely at 0, 30, 60, and 90 min.
  
  
  RESULTS
  There was a significant decrease in clot lysis efficiency observed in presence of iodine containing contrast media comparing to positive control group. Moreover, when the quantity of iodine was increased from 30 mg to 60 mg; the dissolution rate downturned with additional ∼50%.
  
  
  CONCLUSIONS
  In conclusion, our study suggests that high dose of iodine potentially could negatively affect the efficiency of the thrombolytic therapy performed by rt-PA.
authors.affiliation: Department of Radiology, University of Pecs Medical School, Pecs, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Vörös
authors.affiliation: 1st Department of Internal Medicine, University of Pecs Medical School, Pecs, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Deres
authors.affiliation: 1st Department of Internal Medicine, University of Pecs Medical School, Pecs, Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Halmosi
authors.affiliation: Department of Radiology, University of Pecs Medical School, Pecs, Hungary.
authors.forename: Edit
authors.initials: E
authors.lastname: Várady
authors.affiliation: 1st Department of Internal Medicine, University of Pecs Medical School, Pecs, Hungary.
authors.forename: Kálmán
authors.initials: K
authors.lastname: Tóth
authors.affiliation: Department of Radiology, University of Pecs Medical School, Pecs, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Battyáni
mesh_terms: D000818:Animals; D000072226:Computed Tomography Angiography / Q000379:methods*; D003287:Contrast Media / Q000494:pharmacology* / Q000627:therapeutic use; D005343:Fibrinolytic Agents; D006801:Humans; D008297:Male; D051381:Rats; D015912:Thrombolytic Therapy / Q000379:methods*; D010959:Tissue Plasminogen Activator / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D003287:Contrast Media; D005343:Fibrinolytic Agents; D010959:Tissue Plasminogen Activator
keywords: Contrast media; computed tomography; rt-PA; thrombolytic therapy; thrombus