title: SIRT1 promotes the proliferation and metastasis of human pancreatic cancer cells.
abstract: SIRT1 plays an important role in human malignant progression, inducing cancer cell proliferation and metastasis by regulating downstream gene expressions. However, little is known about the underlying mechanisms in which SIRT1 promotes pancreatic cancer tumorigenesis. The aim of this study is to investigate the SIRT1 expression levels and biological functions in promoting pancreatic cancer progression. We first investigated the expression of SIRT1 in a series of pancreatic cancer tissues as well as in a panel of pancreatic cancer cell lines. The effect of SIRT1 on cell activity was explored by knockdown experiments. Cell growth was measured using the MTT assay and colony-formation assay. Migration and invasion were tested using transwell assay. Our results showed that the expression of SIRT1 was significantly up-regulated both in pancreatic cancer tissues and cell lines. Knockdown of SIRT1 suppressed cell proliferation and migration of pancreatic cancer cells. This is the first report to disclose the role of SIRT1 in regulation of pancreatic cancer cell proliferation and migration, which may provide a potential therapeutic target for pancreatic cancer patients.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Jin
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Chu
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ma
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
authors.forename: Yuanpu
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D056564:Sirtuin 1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: SIRT1; migration; pancreatic carcinoma; proliferation