title: Characterization of three commercial ELISA kits for detection of BOHV-1 gE specific antibodies in serum and milk samples and applicability of bulk milk for determination of herd status.
abstract: Vaccination of animals with gE-deleted vaccine strains (gE- marker vaccines) and differential detection of vaccinated vs infected animals with antibody ELISA targeting the gE or the gB proteins have been proved to be useful tools in programs for control and eradication of the bovine herpesvirus 1 (BoHV-1) responsible for infectious bovine rhinotracheitis (IBR), a major pathogen of cattle. The diagnostic sensitivity (DSe) and specificity (DSp) of three commercial gE ELISA kits from IDEXX, IDVet and CIV-HIPRA were compared for serum and milk matrices. Limiting the analysis to 198 individual with concordant ELISA results in serum (91 naïve, 37 vaccinated and 70 infected) the DSe of gE kits was estimated to 0,97 for IDEXX, 0,93 for CIV-HIPRA and 0,53 for IDVet using milk samples and the DSp to 0,95 for IDEXX, 1,00 for IDVet and CIV-HIPRA. The applicability of gE ELISA for individual or bulk milk testing as an additional tool in control programs dedicated to the certification and control of vaccinated herds was evaluated. Two of the three evaluated gE ELISA kits presented substantial to good agreement individual milk and serum samples. The bulk-tank milk also proved to be suitable for the detection of BoHV-1 in vaccinated herds provided that gE prevalence is superior to 10% as false negative results are often observed at lower gE herd prevalence. This limitation could be reduced to 8% of prevalence when a prior concentration step was applied to bulk milk samples.
authors.affiliation: Department Virology, Veterinary and Agrochemical Research Center (CODA-CERVA), Groeselenberg 99, B-1180 Brussels, Belgium. Electronic address: marylene.tignon@coda-cerva.be.
authors.forename: Marylène
authors.initials: M
authors.lastname: Tignon
authors.affiliation: Department Virology, Veterinary and Agrochemical Research Center (CODA-CERVA), Groeselenberg 99, B-1180 Brussels, Belgium.
authors.forename: Miet
authors.initials: M
authors.lastname: De Baere
authors.affiliation: Department Virology, Veterinary and Agrochemical Research Center (CODA-CERVA), Groeselenberg 99, B-1180 Brussels, Belgium.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Hanon
authors.affiliation: Animal Health Care Flanders (DGZ), Industrielaan 29, B-8820 Torhout, Belgium.
authors.forename: Annelies
authors.initials: A
authors.lastname: Goolaerts
authors.affiliation: Regional Association for Animal Registration and Health (ARSIA), Allée des Artisans 2, B-5590 Ciney, Belgium.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Houtain
authors.affiliation: Regional Association for Animal Registration and Health (ARSIA), Allée des Artisans 2, B-5590 Ciney, Belgium.
authors.forename: Laurent
authors.initials: L
authors.lastname: Delooz
authors.affiliation: Department Virology, Veterinary and Agrochemical Research Center (CODA-CERVA), Groeselenberg 99, B-1180 Brussels, Belgium.
authors.forename: Ann Brigitte
authors.initials: AB
authors.lastname: Cay
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005260:Female; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D008892:Milk / Q000276:immunology*; D012680:Sensitivity and Specificity; D022581:Vaccines, Marker / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D022581:Vaccines, Marker; D014764:Viral Proteins
keywords: Bovine herpesvirus 1 (BoHV-1); Bulk tank milk; ELISA; Infectious bovine rhinotracheitis (IBR); Milk concentration; Serological diagnosis