title: Effects of Salt Concentrations and Nitrogen and Phosphorus Starvations on Neutral Lipid Contents in the Green Microalga Dunaliella tertiolecta.
abstract: Dunaliella tertiolecta, a halotolerant alga, can accumulate large amounts of neutral lipid, which makes it a potential biodiesel feedstock. In this study, neutral lipids of D. tertiolecta induced by different salinities or N or P starvation were analyzed by thin-layer chromatography (TLC), flow cytometry (FCM), and confocal laser scanning microscopy (CLSM). High salinities or N or P starvation resulted in a decrease in cell growth and chlorophyll contents of D. tertiolecta. Neutral lipid contents increased markedly after 3-7 days of N starvation or at low NaCl concentrations (0.5-2.0 M). N starvation had a more dramatic effect on the neutral lipid contents of D. tertiolecta than P starvation. Four putative ME isozymes in different conditions can be detected by using isozyme electrophoresis. Two alternative acetyl-CoA producers, ACL and ACS genes, were up-regulated under low salinities and N starvation. It was suggested that low salinities and N starvation are considered efficient ways to stimulate lipid accumulation in D. tertiolecta.
authors.affiliation: College of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Liang
authors.affiliation: College of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Qv
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan 430072, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan 430072, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Jian-Guo
authors.identifier: http://orcid.org/0000-0002-3361-6149
authors.initials: JG
authors.lastname: Jiang
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D058086:Microalgae / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism*
chemical_list: D008055:Lipids; D002734:Chlorophyll; D010758:Phosphorus; D012965:Sodium Chloride; D009584:Nitrogen
keywords: Dunaliella tertiolecta; flow cytometry; neutral lipid; nitrogen starvation; phosphorus starvation