title: Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum(L.) L.
abstract: In this study, we report phytoremediation of textile dyes using hairy roots derived throughAgrobacterium rhizogenes(NCIM 5140) infection of in vitro leaf and stem explants of a halophyteSesuvium portulacastrum(L.) L. Leaf explants showed higher frequency of hairy root induction (70%) than stem explants (30%), and maximum number of roots (leaf 42.3 ± 2.4 and stem 50.3 ± 1.7). Transformed nature of hairy roots was ascertained by amplifying 970 bp region of T-DNA of Ri plasmid. Hairy roots were screened for phytoremediation of various textile dyes and results showed that HRs were able to degrade Reactive green 19A HE4BD upto 98% within 5 days of incubation. Spectrophotometric analysis showed decrease in dye concentration while HPLC and FTIR analysis confirmed its degradation. Seed germination assay demonstrated non-toxic nature of the extracted metabolites. This is the first report on induction of hairy root culture inSesuvium portulacastrumand phytoremediation of textile dyes.
authors.affiliation: Shri Shiv Chhatrapati College of Arts, Commerce and Science, Bodkenagar, Junnar, Pune 410 502, India.
authors.forename: Vinayak H
authors.initials: VH
authors.lastname: Lokhande
authors.affiliation: School of Biotechnology and Bioinformatics, Dr. D. Y. Patil University, C. B. D. Belapur, Navi Mumbai 400 614, India.
authors.forename: Subhash
authors.initials: S
authors.lastname: Kudale
authors.affiliation: Plant Stress Physiology and Biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India; Department of Botany, Savitribai Phule Pune University, Pune 411 007, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Nikalje
authors.affiliation: School of Biotechnology, Amity University, Mumbai 410 210, India.
authors.forename: Neetin
authors.initials: N
authors.lastname: Desai
authors.affiliation: Plant Stress Physiology and Biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: Penna
authors.initials: P
authors.lastname: Suprasanna
keywords: ABTS, 2,20-azinobis,3-ethylben-zothiazoline-6-sulfonic acid; Agrobacterium rhizogenes; BA, benzyl adenine; FTIR, Fourier transform infrared spectroscopy; HRs, hairy roots; Hairy root induction; MS, Murashige and Skoog; PCR, polymerase chain reaction; Phytoremediation; Sesuvium portulacastrum (L.) L.; Textile dye; UV–vis, ultra violet visible