title: Accurate Size and Size-Distribution Determination of Polystyrene Latex Nanoparticles in Aqueous Medium Using Dynamic Light Scattering and Asymmetrical Flow Field Flow Fractionation with Multi-Angle Light Scattering.
abstract: Accurate determination of the intensity-average diameter of polystyrene latex (PS-latex) by dynamic light scattering (DLS) was carried out through extrapolation of both the concentration of PS-latex and the observed scattering angle. Intensity-average diameter and size distribution were reliably determined by asymmetric flow field flow fractionation (AFFFF) using multi-angle light scattering (MALS) with consideration of band broadening in AFFFF separation. The intensity-average diameter determined by DLS and AFFFF-MALS agreed well within the estimated uncertainties, although the size distribution of PS-latex determined by DLS was less reliable in comparison with that determined by AFFFF-MALS.
authors.affiliation: Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. H-kato@aist.go.jp.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kato
authors.affiliation: Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. nakamura@tasc-nt.or.jp.
authors.forename: Ayako
authors.initials: A
authors.lastname: Nakamura
authors.affiliation: Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. kayori.takahashi@ni.aist.go.jp.
authors.forename: Kayori
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. s.kinugasa@aist.go.jp.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kinugasa
keywords: asymmetric flow field flow fractionation; dynamic light scattering; multi-angle light scattering; nanoparticle; polystyrene latex