title: Integrated immunochromatographic strip with glucometer readout for rapid quantification of phosphorylated proteins.
abstract: A new technology to quantify phospho-p5315by converting its content to the amount of glucose which is detectable by a glucometer was developed. An immunochromatographic test strip (ITS) was used as a disposable platform, where primary antibody (Ab1)-modified Fe3O4magnetic nanoparticles (Fe3O4-Ab1) were settled on the test zone to capture both the target phospho-p5315and the detection antibody (Ab2)-glucose encapsulating liposome (GEL) conjugate. The measurement was based on the release and subsequent detection of glucose from Ab2-GEL using a glucose meter (GM). The amount of glucose is proportional to the phospho-p5315concentration from 0.1 to 50 ng mL-1, the limit of detection is 50 pg mL-1(3S/N). The high sensitivity was a result of the huge number of glucose encapsulated in the liposome. Taking the advantage of low cost, widespread availability and portability of the test trip, together with the personal GM, the presented approach can be easily used to detect other disease biomarkers in medical diagnostics and environmental monitoring.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China; School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lin
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China; School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA. Electronic address: dan.du@mail.ccnu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Du
authors.affiliation: School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA. Electronic address: yuehe.lin@wsu.edu.
authors.forename: Yuehe
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004209:Disposable Equipment; D005947:Glucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry; D010750:Phosphoproteins / Q000378:metabolism*; D011934:Reagent Strips; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D008081:Liposomes; D058185:Magnetite Nanoparticles; D010750:Phosphoproteins; D011934:Reagent Strips; D016159:Tumor Suppressor Protein p53; D005947:Glucose
keywords: Glucometer; Immunochromatographic test strip; Liposome; Phospho-p53(15)