title: Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants.
abstract: This study characterized the viral small interfering RNAs (vsiRNAs) from Nicotiana tabacum cv. Samsun infected with a cucumber mosaic virus (CMV) 2b-deficient mutant. Most vsiRNAs were 21 -22 nucleotides in length and the 5'-terminal ends were dominated by A and U, respectively. The observed vsiRNAs were heterogeneously distributed throughout the CMV genome; however, most of the vsiRNAs were derived from sense strands, as opposed to antisense strands. These results demonstrate the conserved and specific function of Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins in tobacco. Finally, it was revealed that vsiRNAs target abundant host genes, indicating complex roles for CMV vsiRNAs during the development of symptoms.
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Ronghua South Street No. 11, Beijing, 100176, People's Republic of China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Ronghua South Street No. 11, Beijing, 100176, People's Republic of China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: China Agricultural University, Yuanmingyuan West Street No. 2, Beijing, 100129, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Ronghua South Street No. 11, Beijing, 100176, People's Republic of China. zhusf@caiq.gov.cn.
authors.forename: Shuifang
authors.identifier: http://orcid.org/0000-0002-3414-7698
authors.initials: S
authors.lastname: Zhu
mesh_terms: D017799:Cucumovirus / Q000235:genetics* / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D016679:Genome, Viral; D054884:Host-Pathogen Interactions / Q000235:genetics; D010935:Plant Diseases / Q000821:virology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D014026:Nicotiana / Q000821:virology*
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase