title: Use of microfluidics to assess the platelet-based control of coagulation.
abstract: This paper provides an overview of the various types of microfluidic devices that are employed to study the complex processes of platelet activation and blood coagulation in whole blood under flow conditions. We elaborate on how these devices are used to detect impaired platelet-dependent fibrin formation in blood from mice or patients with specific bleeding disorders. We provide a practical guide on how to assess formation of a platelet-fibrin thrombus under flow, using equipment that is present in most laboratories. In addition, we describe current insights on how blood flow and shear rate alter the location of platelet populations, von Willebrand factor, coagulation factors, and fibrin in a growing thrombus. Finally, we discuss possibilities and limitations for the clinical use of microfluidic devices to evaluate a hemostatic or prothrombotic tendency in patient blood samples.
authors.affiliation: a Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM) , Maastricht University , Maastricht , The Netherlands.
authors.forename: Magdolna
authors.initials: M
authors.lastname: Nagy
authors.affiliation: a Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM) , Maastricht University , Maastricht , The Netherlands.
authors.forename: Johan W M
authors.initials: JWM
authors.lastname: Heemskerk
authors.affiliation: a Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM) , Maastricht University , Maastricht , The Netherlands.|b Department of Bioanalytics , Leibniz Institute for Analytical Sciences - ISAS- e.V. , Dortmund , Germany.
authors.forename: Frauke
authors.initials: F
authors.lastname: Swieringa
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D013927:Thrombosis / Q000378:metabolism* / Q000473:pathology
chemical_list: D001779:Blood Coagulation Factors; D005337:Fibrin
keywords: Fibrin; platelet; thrombin; thrombus