title: Atmospheric Pressure Pulsed Plasma Induces Cell Death in Photosynthetic Organs via Intracellularly Generated ROS.
abstract: The toxicity of atmospheric-pressure pulsed plasma on plant leaf tissues is studied. A nanosecond-pulsed plasma jet is applied to Arabidopsis thaliana leaves. In case of cotyledon, cell death is induced by treatment of only a few seconds. Cell death is also induced in the adult leaf by only 5 seconds of plasma treatment. Plasma induced reactive oxygen species (ROS) accumulation across the tissues within plasma-treated area. Plasma also induced direct physical damage to epidermis tissue of treated area but merely no damage to mesophyll. Thus, we propose direct physical damage in epidermis and ROS accumulation across the treated area induced cell death by plasma treatment. Plasma treatment with same duration in different organ also induced ROS accumulation but not plant death, suggests damage on photosynthetic organ by oxidative stress might be direct reason to induce cell death. We could also observe similar plasma induced death in Solanum esculentum, Petunia axillaris, and Nicotiana benthamiana but death is induced only in treated area. Thus, we propose atmospheric plasma induce oxidative stress in photosynthetic organ to induce cell death in plants.
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
authors.forename: You-Bin
authors.initials: YB
authors.lastname: Seol
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Jaewook
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Electrical Engineering, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
authors.forename: Se-Hong
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea. hychang@kaist.ac.kr.
authors.forename: Hong
authors.initials: H
authors.lastname: Young Chang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism; D001274:Atmospheric Pressure; D016923:Cell Death / Q000187:drug effects*; D002734:Chlorophyll / Q000378:metabolism; D018384:Oxidative Stress; D010788:Photosynthesis; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D058626:Plasma Gases / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D058626:Plasma Gases; D017382:Reactive Oxygen Species; D002734:Chlorophyll