title: HuR Stabilizes lnc-Sox5 mRNA to Promote Tongue Carcinogenesis.
abstract: Long noncoding RNAs (lncRNAs) have been recently regarded as systemic regulators in multiple biological processes including tumorigenesis. In this study, we report an ultra-highly expressed lncRNA, lnc-Sox5, in tongue tumor tissues. The results imply that lnc-Sox5 may play vital role in tongue carcinoma progression. We observed that the growth of Tca8113 cells was suppressed by lnc-Sox5 downregulation. Additionally, lnc-Sox5 knockdown simultaneously increased Tca8113 cell apoptosis, but the cell cycle was arrested. RNA immunoprecipitation suggested that HuR directly bound to and stabilized lnc-Sox5 RNA. Consistently, HuR knockdown reduced the level of lnc-Sox5 in Tca8113 cells. However, overexpression of HuR induced more lnc-Sox5 in Tca8113 cells. Both lnc-Sox5 knockdown and HuR knockdown suppressed Tca8113 cell tumorigenesis in xenograft models. These results suggest that lnc-Sox5, which was stabilized by HuR, could regulate carcinogenesis of tongue cancer and may serve as a predicted target for tongue carcinoma therapies.
authors.affiliation: Qingdao University, Qingdao, 266000, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Wang
authors.forename: Shucheng
authors.initials: S
authors.lastname: Ye
authors.forename: Junye
authors.initials: J
authors.lastname: Wang
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Gu
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Xuezhen
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D000067780:ELAV-Like Protein 1; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D055755:SOXD Transcription Factors / Q000235:genetics*; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C490301:SOX5 protein, human; D055755:SOXD Transcription Factors