title: Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor.
abstract: A novel amperometric glucose biosensor was fabricated by layer-by-layer self-assembly of gold nanorods (AuNRs) and glucose oxidase (GOD) onto single-walled carbon nanotubes (SWCNTs)-functionalized three-dimensional sol-gel matrix. A thiolated aqueous silica sol containing SWCNTs was first assembled on the surface of a cleaned Au electrode, and then the alternate self-assembly of AuNRs and GOD were repeated to assemble multilayer films of AuNRs-GOD onto SWCNTs-functionalized silica gel for optimizing the biosensor. Among the resulting glucose biosensors, the four layers of AuNRs-GOD-modified electrode showed the best performance. The sol-SWCNTs-(AuNRs- GOD)₄/Au biosensor exhibited a good linear range of 0.01-8 mM glucose, high sensitivity of 1.08 μA/mM, and fast amperometric response within 4 s. The good performance of the proposed glucose biosensor could be mainly attributed to the advantages of the three-dimensional sol-gel matrix and stereo self-assembly films, and the natural features of one-dimensional nanostructure SWCNTs and AuNRs. This study may provide a new facile way to fabricate the enzyme-based biosensor with high performance.
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. wubaoyan@scnu.edu.cn.
authors.forename: Baoyan
authors.initials: B
authors.lastname: Wu
authors.affiliation: School of Electronic and Information Engineering, South China University of Technology, Guangzhou 510640, China. shhou@scut.edu.cn.
authors.forename: Shihua
authors.initials: S
authors.lastname: Hou
authors.affiliation: The key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. mzy@mail.nankai.edu.cn.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Miao
authors.affiliation: The key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. zhangcong0672@163.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. jiyh@scnu.edu.cn.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ji
keywords: biosensor; carbon nanotubes; gold nanorods (AuNRs); layer-by-layer; sol-gel