title: Prevalence of Diarrheagenic Escherichia coli in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan.
abstract: The source and routes of diarrheagenic Escherichia coli (DEC) remain poorly understood. To investigate the involvement of domestic animals in the dissemination of DEC, the prevalence of DEC in foods and fecal specimens from cattle, pigs, chickens, healthy carriers, and patients in Osaka and Hyogo, Japan was investigated using a multiplex real-time Polymerase Chain Reaction assay. The most abundant virulence genes were astA and eae, which had a prevalence 46.8% and 27.4%, respectively. Additionally, stx1 (26.6%) and stx2 (45.9%) were prevalent in cattle feces, while est (8.5%) and elt (7.6%) were prevalent in pig feces. afaB was the second-most prevalent gene in patients and healthy carriers, and it had detection rates of 5.1% and 8.1%, respectively. In contrast, afaB was not detected in animal feces or foods, except for three porcine fecal samples. The aggR gene was more prevalent in humans than in foods or animal feces. Both Shiga toxin-producing E. coli and atypical enteropathogenic E. coli carried by cattle may be sources for diarrheal diseases in humans. Pigs may be a source for human enterotoxigenic E. coli infections, whereas humans are expected to be the reservoir for diffusely adhering E. coli, enteroaggregative E. coli, and enteroinvasive E. coli.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology.|Graduate School of Human Life Science, Osaka City University.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Graduate School of Human Life Science, Osaka City University.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Graduate School of Human Life Science, Osaka City University.
authors.forename: Dongming
authors.initials: D
authors.lastname: Zheng
authors.affiliation: National Hospital Organization Osaka Minami Medical Center.
authors.forename: Sami
authors.initials: S
authors.lastname: Fujihara
authors.affiliation: Hyogo Prefecture Tajima Meat Hygiene Inspection Office.
authors.forename: Akiyo
authors.initials: A
authors.lastname: Wakabayashi
authors.affiliation: Hyogo Prefecture Tajima Meat Hygiene Inspection Office.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Okahata
authors.affiliation: Hyogo Prefecture Tajima Meat Hygiene Inspection Office.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Osaka Municipal Meat Inspection Center.
authors.forename: Atsunori
authors.initials: A
authors.lastname: Saeki
authors.affiliation: Osaka City Institute of Public Health and Environmental Sciences.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: National Institute of Health Sciences.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hara-Kudo
authors.affiliation: Graduate School of Human Life Science, Osaka City University.|The OCU Advanced Research Institute for Natural Science and Technology, Osaka City University.
authors.forename: Eriko
authors.initials: E
authors.lastname: Kage-Nakadai
authors.affiliation: Graduate School of Human Life Science, Osaka City University.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D060885:Multiplex Polymerase Chain Reaction; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D037521:Virulence Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors
keywords: Diarrheagenic E.coli; multiplex real-time PCR; reservoir; screening