title: Increased Numbers of CD4+CD25+and CD8+CD25+T-Cells in Peripheral Blood of Patients with Rheumatoid Arthritis with Parvovirus B19 Infection.
abstract: To investigate T-cell subpopulations in peripheral blood of human parvovirus B19 DNA-positive (B19+) and -negative (B19-) patients with rheumatoid arthritis (RA) and healthy persons.
  
  
  
  Blood samples were collected from 115 patients with RA and 47 healthy volunteers; 27 patients with RA and nine controls were B19+Cluster of differentiation (CD) 4, 8, 25 and 45RA were analyzed on blood cells. CD25 expression on CD4+CD45RA+, CD4+CD45RA-, CD8+CD45RA+, CD8+CD45RA-subsets were analyzed by flow cytometry.
  
  
  
  The percentage of CD25lowand CD25hicells was increased on CD4+CD45RA+, CD4+CD45RA-T-cells and the percentage of CD25+cells was increased on CD8+CD45RA+, CD8+CD45RA-T-cells of B19+patients with RA in comparison with B19-patients and controls.
  
  
  
  Raised levels of CD4 and CD8 regulatory T-cells in B19+RA patients could cause down-regulation of antiviral clearance mechanisms and lead to activation of persistent human parvovirus B19 infection in patients with RA.
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Milda
authors.initials: M
authors.lastname: Naciute
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Maciunaite
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Diana
authors.initials: D
authors.lastname: Mieliauskaite
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.|Centre of Rheumatology, Vilnius University, Vilnius, Lithuania.
authors.forename: Rita
authors.initials: R
authors.lastname: Rugiene
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Aukse
authors.initials: A
authors.lastname: Zinkeviciene
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Mykolas
authors.initials: M
authors.lastname: Mauricas
authors.affiliation: A. Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Riga, Latvia.
authors.forename: Modra
authors.initials: M
authors.lastname: Murovska
authors.affiliation: Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania igirkontaite@yahoo.com.
authors.forename: Irute
authors.initials: I
authors.lastname: Girkontaite
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology* / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology* / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D016732:Parvovirus B19, Human / Q000276:immunology* / Q000502:physiology
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D017493:Leukocyte Common Antigens
keywords: CD4+CD25+; CD8+CD25+; Parvovirus B19; Treg; rheumatoid arthritis