title: Association of Functional Variants of Phase I and II Genes with Chronic Obstructive Pulmonary Disease in a Serbian Population.
abstract: BACKGROUND
  Chronic obstructive pulmonary disease (COPD) is a complex disorder characterized by increased oxidative stress. Functional genetic variants of phase I and II genes are implicated in oxidants-antioxidants imbalance and may be involved in COPD development. In this study, we aimed to investigate the role of cytochrome P450 (CYP), glutathione S-transferase (GST) and microsomal epoxide hydrolase (mEH) functional variants in the pathogenesis of COPD in a Serbian population.
  
  
  METHODS
  The genotypes of 122 COPD patients and 100 controls with normal lung function were determined for CYP1A1 *1A/*2A, CYP2E1 *1A/*5B, GSTM1 null, GSTT1 null GSTP1 Ile105Val, mEH Tyr113His and mEH His139Arg gene variants.
  
  
  RESULTS
  Results obtained showed that GSTM1 null variant was significantly more represented in COPD patients than in controls (61.5% vs. 47.0%; OR=1.80; p=0.042). Also, a significant difference was observed for combinations of GSTM1 null and GSTP1 105Val/(Val) (38.5% vs. 24.0%; OR=1.98; p=0.029), as well as for CYP1A1 *1A/*2A, GSTM1 null and mEH 113His/(His) genotypes (7.4% vs. 1.0%; OR=7.88; p=0.025).
  
  
  CONCLUSIONS
  These are the first data concerning the analysis of the variants of phase I and II genes in the pathogenesis of COPD in a Serbian population. Results obtained in this study open up the possibility for thorough analyses of the role of genetic factors in COPD on larger cohorts. Also, they implicate the importance of previously described genetic associations with COPD in our population, as well as reveal a new one, not reported so far.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Stanković
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nikolić
authors.affiliation: Novartis International AG, Novartis Campus, Forum 1, Basel, Switzerland.
authors.forename: Andrija
authors.initials: A
authors.lastname: Tomović
authors.affiliation: Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Mitić-Milikić
authors.affiliation: Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia.
authors.forename: Ljudmila
authors.initials: L
authors.lastname: Nagorni-Obradović
authors.affiliation: Zvezdara University Medical Centre, Belgrade, Serbia; University of Belgrade, School of Dentistry, Department of Internal Medicine, Belgrade, Serbia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Petrović-Stanojević
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Dragica
authors.initials: D
authors.lastname: Radojković
keywords: COPD; candidate gene association study; genetic variation; oxidative stress