title: Zerumbone Alleviates Neuropathic Pain through the Involvement of l-Arginine-Nitric Oxide-cGMP-K⁺ ATP Channel Pathways in Chronic Constriction Injury in Mice Model.
abstract: The present study investigates the involvement of the l-arginine-Nitric Oxide-cGMP-K⁺ ATP pathways responsible for the action of anti-allodynic and antihyperalgesic activities of zerumbone in chronic constriction injury (CCI) induced neuropathic pain in mice. The role of l-arginine-NO-cGMP-K⁺ was assessed by the von Frey and the Randall-Selitto tests. Both allodynia and hyperalgesia assessments were carried out on the 14th day post CCI, 30 min after treatments were given for each respective pathway. Anti-allodynic and antihyperalgesic effects of zerumbone (10 mg/kg, i.p) were significantly reversed by the pre-treatment of l-arginine (10 mg/kg), 1H [1,2,4]Oxadiazole[4,3a]quinoxalin-1-one (ODQ), a soluble guanosyl cyclase blocker (2 mg/kg i.p.) and glibenclamide (ATP-sensitive potassium channel blocker) (10 mg/kg i.p.) (p< 0.05). Taken together, these results indicate that systemic administration of zerumbone produces significant anti-allodynic and antihyperalgesic activities in neuropathic pain in mice possibly due to involvement of the l-arginine-NO-cGMP-PKG-K⁺ ATP channel pathways in CCI model.
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. atiqahzulazmi@yahoo.com.
authors.forename: Nurul Atiqah
authors.initials: NA
authors.lastname: Zulazmi
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. banulatagopalsamy@gmail.com.
authors.forename: Banulata
authors.initials: B
authors.lastname: Gopalsamy
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. enimsajsaj@gmail.com.
authors.forename: Jasmine Chia Siew
authors.initials: JC
authors.lastname: Min
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. ahmadakira@upm.edu.my.
authors.forename: Ahmad Akira Omar
authors.initials: AA
authors.lastname: Farouk
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. mrs@upm.edu.
authors.forename: Mohd Roslan
authors.initials: MR
authors.lastname: Sulaiman
authors.affiliation: Department of Biomedical Sciences, School of Diagnostic and Applied Sciences, Faculty of Health Sciences, University Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. hema@ukm.edu.my.
authors.forename: B Hemabarathy
authors.initials: BH
authors.lastname: Bharatham
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. enoch@upm.edu.my.
authors.forename: Enoch Kumar
authors.initials: EK
authors.lastname: Perimal
mesh_terms: D000700:Analgesics / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000378:metabolism; D003250:Constriction; D006152:Cyclic GMP / Q000378:metabolism; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000188:drug therapy* / Q000378:metabolism; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D051379:Mice; D009437:Neuralgia / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010147:Pain Measurement; D012717:Sesquiterpenes / Q000008:administration & dosage* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000700:Analgesics; D054086:KATP Channels; D012717:Sesquiterpenes; D009569:Nitric Oxide; C403304:zerumbone; D001120:Arginine; D006152:Cyclic GMP
keywords: NO-cGMP pathway; chronic constriction injury (CCI); neuropathic pain; nitric oxide; zerumbone