title: Nose to brain delivery in rats: Effect of surface charge of rhodamine B labeled nanocarriers on brain subregion localization.
abstract: Nose to brain delivery and nanotechnology are the combination of innovative strategies for molecules to reach the brain and to bypass blood brain barriers. In this work we investigated the fate of two rhodamine B labeled polymeric nanoparticles (Z-ave <250nm) of opposite surface charge in different areas of the brain after intranasal administration in rats. A preliminary screening was carried out to select the suitable positive (chitosan/poly-l-lactide-co-glycolide) nanocarrier through photon correlation spectroscopy and turbiscan. Physico-chemical and technological characterizations of poly-l-lactide-co-glycolide (negative) and chitosan/poly-l-lactide-co-glycolide (positive) fluorescent labeled nanoparticles were performed. The animals were allocated to three groups receiving negative and positive polymeric nanoparticles via single intranasal administration or no treatment. The localization of both nanocarriers in different brain areas was detected using fluorescent microscopy. Our data revealed that both nanocarriers reach the brain and are able to persist in the brain up to 48h after intranasal administration. Surface charge influenced the involved pathways in their translocation from the nasal cavity to the central nervous system. The positive charge of nanoparticles slows down brain reaching and the trigeminal pathway is involved, while the olfactory pathway may be responsible for the transport of negatively charged nanoparticles, and systemic pathways are not excluded.
authors.affiliation: Laboratory of Drug Delivery Technology, Department of Drug Science, University of Catania, Via S. Sofia 64, 95125 Catania, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bonaccorso
authors.affiliation: Laboratory of Drug Delivery Technology, Department of Drug Science, University of Catania, Via S. Sofia 64, 95125 Catania, Italy. Electronic address: teresa.musumeci@unict.it.
authors.forename: T
authors.initials: T
authors.lastname: Musumeci
authors.affiliation: Department of Biomedical and Biotechnological Science, University of Catania, Via S. Sofia 64, 95125 Catania, Italy.
authors.forename: M F
authors.initials: MF
authors.lastname: Serapide
authors.affiliation: Institute of Neurological Sciences, CNR, Section of Catania, Via P. Gaifami 18, 95126 Catania, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Pellitteri
authors.affiliation: UCL, School of Pharmacy, University College London, 29_39 Brunswick Square, London WC1N 1AX, United Kingdom.
authors.forename: I F
authors.initials: IF
authors.lastname: Uchegbu
authors.affiliation: Laboratory of Drug Delivery Technology, Department of Drug Science, University of Catania, Via S. Sofia 64, 95125 Catania, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Puglisi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D009297:Nasal Mucosa / Q000378:metabolism*; D009833:Olfactory Pathways / Q000378:metabolism; D011098:Polyglactin 910 / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines / Q000737:chemistry / Q000493:pharmacokinetics; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D012235:Rhodamines; D011098:Polyglactin 910; D048271:Chitosan; C029773:rhodamine B
keywords: Brain; Chitosan; Fluorescent microscopy; In vivo study; Intranasal delivery; Nanoparticles; PLGA; Rats