title: Attenuation of Focal Adhesion Kinase Reduces Lipopolysaccharide-Induced Inflammation Injury through Inactivation of the Wnt and NF-κB Pathways in A549 Cells.
abstract: Overall analysis and understanding of mechanisms are of great importance for treatment of infantile pneumonia due to its high morbidity and mortality worldwide. In this study, we preliminarily explored the function and mechanism of focal adhesion kinase (FAK) in regulation of inflammatory response induced by lipopolysaccharides in A549 cells. Flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, quantitative reverse transcription polymerase chain reaction, and Western blot analysis were used to explore the correlation of FAK expression with cell apoptosis, viability, and the inflammatory cytokine activity in A549 cells. The results showed that knockdown of FAK enhanced cell viability, suppressed apoptosis, and decreased inflammatory cytokine activity. In addition, downregulation of FAK could activate the Wnt and nuclear factor κB signaling pathways. These findings suggest that FAK might be involved in progression of infantile pneumonia and could be a new therapeutic target for this disease.
authors.affiliation: Jining No. 1 People's Hospital, Department of Pediatrics, Jining, 272011, China. zhuliping5280@126.com.
authors.forename: D
authors.initials: D
authors.lastname: Bai
authors.forename: S
authors.initials: S
authors.lastname: Cong
authors.forename: L P
authors.initials: LP
authors.lastname: Zhu
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000633:toxicity*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051153:Wnt Proteins / Q000187:drug effects* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D051153:Wnt Proteins; D051416:Focal Adhesion Protein-Tyrosine Kinases