title: Developmental patterning of the sub-epidermal integument cell layer inArabidopsisseeds.
abstract: Angiosperm seed development is a paradigm of tissue cross-talk. Proper seed formation requires spatial and temporal coordination of the fertilization products - embryo and endosperm - and the surrounding seed coat maternal tissue. In earlyArabidopsisseed development, all seed integuments were thought to respond homogenously to endosperm growth. Here, we show that the sub-epidermal integument cell layer has a unique developmental program. We characterized the cell patterning of the sub-epidermal integument cell layer, which initiates a previously uncharacterized extra cell layer, and identified TRANSPARENT TESTA 16 and SEEDSTICK MADS box transcription factors as master regulators of its polar development and cell architecture. Our data indicate that the differentiation of the sub-epidermal integument cell layer is insensitive to endosperm growth alone and to the repressive mechanism established by FERTILIZATION INDEPENDENT ENDOSPERM and MULTICOPY SUPPRESSOR OF IRA1 Polycomb group proteins. This work demonstrates the different responses of epidermal and sub-epidermal integument cell layers to fertilization.
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France.|Ecole Doctorale 567 Sciences du Végétal, University Paris-Sud, University of Paris-Saclay, bat 360, Orsay Cedex 91405, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Coen
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France.
authors.forename: Elisa
authors.initials: E
authors.lastname: Fiume
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: De Vos
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France.|Ecole Doctorale 567 Sciences du Végétal, University Paris-Sud, University of Paris-Saclay, bat 360, Orsay Cedex 91405, France.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.affiliation: INRA, Génétique Animale et Biologie Intégrative, Domaine de Vilvert, Jouy-en-Josas Cedex 78352, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Pechoux
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Lepiniec
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, University of Paris-Saclay, Route de St-Cyr (RD10), Versailles Cedex 78026, France enrico.magnani@inra.fr.
authors.forename: Enrico
authors.identifier: 0000-0002-2959-3714
authors.initials: E
authors.lastname: Magnani
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000196:embryology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D019521:Body Patterning; D002454:Cell Differentiation; D005306:Fertilization; D063245:Plant Development; D019441:Plant Epidermis / Q000166:cytology* / Q000196:embryology*; D012639:Seeds / Q000196:embryology*
chemical_list: D029681:Arabidopsis Proteins
keywords: Fertilization; Integuments; Seed coat; Seed development; Tissue cross-talk