title: A PCR-Based Molecular Detection of Strongyloides stercoralisin Human Stool Samples from Tabriz City, Iran.
abstract: Strongyloides stercoralisis a nematode causing serious infections in immunocompromised patients. In chronically infected patients, the low parasitic content as well as the resemblance of the larvae to several other species make diagnosis basedonmorphology difficult. In the present study, a PCR-based method targeting the internal transcribed sequence 2 (ITS2) of the rDNA region was examined for the molecular detection ofS. stercoralisinfection from the stool samples. A total of 1800 patients were included. Three fresh stool samples were collected per patient, andS. stercoralisisolates were identified by the morphological method. A subset of isolates was later used in the PCR-based method as positive controls. Additionally, negative and no-template controls were included. Data analysis was accomplished using anx²test. Ap-value less than 0.05 was considered significant. In total, fivestool samples were found to be infected withS. stercoralisusing the morphology method. PCR method detectedS. stercoralisDNA target from all of the fiveDNA samples extracted from positive fecal samples.
  
  
  CONCLUSIONS
  The PCR method used for amplifying a short fragment was successful for diagnosis ofS. stercoralisin fecal samples and can be reliable for directly detecting the parasite bypassing morphological method.
authors.affiliation: Department of Parasitology& Mycology, School of Medicine, Arak University of Medical Sciences, Arak 3848176941, Iran. Dr.ghasemikhah@arakmu.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghasemikhah
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran. Tabatabaiefar@gmail.com.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Tabatabaiefar
authors.affiliation: Departments of Parasitology and Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz 3848176941, Iran. a.shariatzadeh@yahoo.com.
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Shariatzadeh
authors.affiliation: Departments of Parasitology and Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz 3848176941, Iran. shahbazi42@yahoo.com.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shahbazi
authors.affiliation: Departments of Parasitology and Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz 3848176941, Iran. hazratiant@tbzmed.ac.ir.
authors.forename: Teymour
authors.initials: T
authors.lastname: Hazratian
keywords: PCR; Strongyloides stercoralis; molecular detection