title: Expression of CRM1 and CDK5 shows high prognostic accuracy for gastric cancer.
abstract: OBJECTIVE
  To evaluate the predictive value of the expression of chromosomal maintenance (CRM)1 and cyclin-dependent kinase (CDK)5 in gastric cancer (GC) patients after gastrectomy.
  
  
  METHODS
  A total of 240 GC patients who received standard gastrectomy were enrolled in the study. The expression level of CRM1 and CDK5 was detected by immunohistochemistry. The correlations between CRM1 and CDK5 expression and clinicopathological factors were explored. Univariate and multivariate survival analyses were used to identify prognostic factors for GC. Receiver operating characteristic analysis was used to compare the accuracy of the prediction of clinical outcome by the parameters.
  
  
  RESULTS
  The expression of CRM1 was significantly related to size of primary tumor (P= 0.005), Borrmann type (P= 0.006), degree of differentiation (P= 0.004), depth of invasion (P= 0.008), lymph node metastasis (P= 0.013), TNM stage (P= 0.002) and distant metastasis (P= 0.015). The expression of CDK5 was significantly related to sex (P= 0.048) and Lauren's classification (P= 0.011). Multivariate Cox regression analysis identified that CRM1 and CDK5 co-expression status was an independent prognostic factor for overall survival (OS) of patients with GC. Integration of CRM1 and CDK5 expression could provide additional prognostic value for OS compared with CRM1 or CDK5 expression alone (P= 0.001).
  
  
  CONCLUSIONS
  CRM1 and CDK5 co-expression was an independent prognostic factors for GC. Combined CRM1 and CDK5 expression could provide a prognostic model for OS of GC.
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Sun
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Xie
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Hong-Teng
authors.initials: HT
authors.lastname: Xie
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Peng-Chen
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Zhang
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Zheng
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Wang
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Jian-Xian
authors.initials: JX
authors.lastname: Lin
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Long-Long
authors.initials: LL
authors.lastname: Cao
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Huang
authors.affiliation: Yu-Qin Sun, Hong-Teng Xie, Peng-Chen Chen, Xiu-Li Zhang, Yao Lin, College of Life Sciences, Fujian Normal University, Fuzhou 350108, Fujian Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000230:Adenocarcinoma / Q000401:mortality* / Q000473:pathology / Q000601:surgery; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D051360:Cyclin-Dependent Kinase 5 / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005743:Gastrectomy; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D028884:Karyopherins / Q000032:analysis* / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis* / Q000378:metabolism; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000401:mortality* / Q000473:pathology / Q000601:surgery; D000097606:Exportin 1 Protein
chemical_list: D014408:Biomarkers, Tumor; D051360:Cyclin-Dependent Kinase 5; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D000097606:Exportin 1 Protein; C495905:CDK5 protein, human
keywords: CDK5; CRM1; Gastric cancer; Prognosis