title: Co-delivery of insulin-like growth factor 1 receptor specific siRNA and doxorubicin using chitosan-based nanoparticles enhanced anticancer efficacy in A549 lung cancer cell line.
abstract: Here, we investigated the effects of dual delivery of IGF-1R siRNA and doxorubicin by chitosan nanoparticles on viability of A549 lung cancer cells line by utilization of MTT and qRT-PCR. Furthermore apoptosis and migration of treated cells were assessed by Annexin-PI and wound healing assays, respectively. The chitosan nanoparticles had about 176 nm size with zeta potential and polydispersive index about 11 mV and 0.3, respectively. The IGF-1R siRNA had synergistic effect on DOX-induced cytotoxicity and apoptosis in tumour cells. In addition, siRNA/DOX-loaded chitosan nanoparticles could significantly decrease migration and expressions of mmp9, VEGF and STAT3 in A549 cells.
authors.affiliation: a Tuberculosis and Lung Disease Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Student's Research Committee , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Hajar
authors.initials: H
authors.lastname: Shali
authors.affiliation: c Department of Immunology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Shabani
authors.affiliation: d Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Pourgholi
authors.affiliation: d Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Hajivalili
authors.affiliation: d Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Leili
authors.initials: L
authors.lastname: Aghebati-Maleki
authors.affiliation: a Tuberculosis and Lung Disease Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Jadidi-Niaragh
authors.affiliation: d Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
authors.affiliation: e Hematology and Oncology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Movassaghpour Akbari
authors.affiliation: f Pishtaz Teb Diagnostics , Tehran , Iran.|g Faculty of Paramedical Sciences , Alborz University of Medical Sciences , Karaj , Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Younesi
authors.affiliation: h Liver and Gastrointestinal Diseases Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|i Department of Immunology, School of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Yousefi
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D048271:Chitosan / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008175:Lung Neoplasms / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry*; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D017526:Receptor, IGF Type 1 / Q000172:deficiency* / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D004337:Drug Carriers; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; D042461:Vascular Endothelial Growth Factor A; D004317:Doxorubicin; D048271:Chitosan; D017526:Receptor, IGF Type 1; D020780:Matrix Metalloproteinase 9
keywords: Chitosan nanoparticle; IGF-1R; doxorubicin; lung cancer; ss siRNA