title: Preparation of cucumber seed peptide-calcium chelate by liquid state fermentation and its characterization.
abstract: A specific cucumber seed peptide (CSP) was successfully produced by liquid state fermentation with B. subtilis HYT. Then the CSP was divided into four kind molecular weight components (CSP, untreated; CSP1, MW>10kDa; CSP2, 6kDa<MW<10kDa; CSP3, MW<6kDa) by ultrafiltration. Among them, the CSP3 displayed the most excellent ability to chelate with calcium (CSP3-Ca). SEM, EDS, ultra-violet spectroscopy, FTIR and Fluorescence spectroscopy were applied to distinguish the characterizations between CSP3 and CSP3-Ca chelate. The results suggested that the apparent characteristics and internal structure of CSP3 were both changed. There were abundant mental-binding sites presented in CSP3, and the COOH, OH, NH2as well CONH might participate in the coordination with Ca2+to form the CSP3-Ca chelate. Meanwhile, the finding showed that cucumber seeds peptide could chelate with calcium and play the role of novel carries for calcium supplement.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Gao
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address: chenye@tust.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002118:Calcium / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D018553:Cucumis sativus / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D010940:Plant Proteins; D002118:Calcium
keywords: Calcium supplement; Characterization; Cucumber seed peptide; Peptide-calcium chelating