title: Cyclin D1 G870A polymorphism: Association with uterine leiomyoma risk andin silicoanalysis.
abstract: Uterine leiomyoma (UL) is the most common benign tumor causing considerable morbidity during the reproductive years in women. Cyclin D1 (CCND1) is a cell cycle regulatory protein that is required for the G1 phase, and increased expression levels of this protein may affect tumorigenesis. The present study aimed to assess the possible effect of theCCND1G870A polymorphism on UL susceptibility. A total of 154 women with UL and 197 healthy women who were age-, body mass index (BMI)- and ethnicity-matched were genotyped for theCCND1G870A (rs9344) polymorphism using the polymerase chain reaction-restriction fragment length polymorphism method. The effects of G870A transition on the structure of mRNA and proteins of CCND1 was evaluated using bioinformatics tools. The frequency of theCCND1870AA genotype was significantly higher in women with UL compared with the control subjects, and the risk of UL was 1.4-fold higher in women with the AA genotype when compared with the GG genotype before and after adjusting for age, BMI, and ethnicity [odds ratio (OR), 1.4; 95% confidence interval (CI), 1.1-2 (P=0.02)]. The frequency ofCCND1870GA genotype was not significantly different between the two groups. The frequency of theCCND1870A allele was significantly higher in the women with UL when compared with the control subjects (57 vs. 48%; P=0.02). Thein silicoanalysis revealed that the G870A transition may fundamentally alter the structure of the CCND1-mRNA. Thus, the CCND1 870AA genotype was associated with UL susceptibility in a sample of women from the southeast of Iran.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.affiliation: Department of Biostatistics and Epidemiology, School of Public Health, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Shahrakipour
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Hajizadeh
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran; Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Mokhtari
authors.affiliation: Department of Biology, Faculty of Science, University of Zabol, Zabol, Sistan and Baluchestan 9861335856, Iran.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Mousavi
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran; Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
authors.forename: Batool
authors.initials: B
authors.lastname: Teimoori
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Minoo
authors.initials: M
authors.lastname: Yaghmaei
keywords: cyclin D1; gene; in silico; polymorphism; uterine leiomyoma