title: Identification of a novel sequence type ofEscherichia colias the causative agent of pyelonephritis and bloodstream infection.
abstract: BACKGROUND
  Globally, extra-intestinal pathogenicEscherichia coliare one of the predominant causative agents of bacteraemia.
  
  
  METHODS
  This case report outlines a presentation of community-acquired pyelonephritis and secondary bloodstream infection in an 81-year-old man. Laboratory investigations revealed that the causative isolate was a multi-drug-resistantE. coliof a novel multi-locus sequence type. This sequence type (ST) was designated ST-458 and was most closely related to the globally prevalent ST-131 lineage.
  
  
  CONCLUSIONS
  This is the first report of a novelE. coliST, ST-458, which caused pyelonephritis and bacteraemia.
authors.affiliation: Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, School of Medicine, Trinity College , Dublin , Ireland.
authors.forename: Micheál
authors.initials: M
authors.lastname: Mac Aogáin
authors.affiliation: Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, School of Medicine, Trinity College , Dublin , Ireland.
authors.forename: Helen
authors.initials: H
authors.lastname: Miajlovic
authors.affiliation: Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, School of Medicine, Trinity College , Dublin , Ireland.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Moloney
authors.affiliation: Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.
authors.forename: Sanjay H
authors.initials: SH
authors.lastname: Chotirmall
authors.affiliation: Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, School of Medicine, Trinity College , Dublin , Ireland.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Rogers
authors.affiliation: Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, School of Medicine, Trinity College , Dublin , Ireland.
authors.forename: Stephen G J
authors.initials: SG
authors.lastname: Smith
keywords: Antimicrobial resistance; Escherichia coli ST-458; Infected renal parenchymal cyst; Pyelonephritis; Virulence factors; bacteraemia