title: Evaluation of Potential Probiotics Isolated from Human Milk and Colostrum.
abstract: Several studies have demonstrated a diversity of bacterial species in human milk, even in aseptically collected samples. The present study evaluated potential probiotic bacteria isolated from human milk and associated maternal variables. Milk samples were collected from 47 healthy women and cultured on selective and universal agar media under aerobic and anaerobic conditions. Bacterial isolates were counted and identified by Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight mass spectrometry and then tested for probiotic properties. Total bacteria in human milk ranged from 1.5 to 4.0 log10CFU/mL. The higher bacterial counts were found in colostrum (mean = 3.9 log10CFU/mL, 95% CI 3.14-4.22, p = 0.00001). The most abundant species was Staphylococcus epidermidis (n = 76). The potential probiotic candidates were Lactobacillus gasseri (n = 4), Bifidobacterium breve (n = 1), and Streptococcus salivarius (n = 4). Despite the small sample size, L. gasseri was isolated only in breast milk from mothers classified into a normal weight range and after a vaginally delivered partum. No potential probiotics showed antagonism against pathogens, but all of them agglutinated different pathogens. Nine bacterial isolates belonging to the species L. gasseri, B. breve, and S. salivarius were selected as potential probiotics. The present study confirms the presence in breast milk of a bacterial microbiota that could be the source of potential probiotic candidates to be used in the formula of simulated maternal milk.
authors.affiliation: Laboratory of Biotherapeutic Agents, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Quésia S
authors.initials: QS
authors.lastname: Damaceno
authors.affiliation: Laboratory of Biotherapeutic Agents, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Jaqueline P
authors.initials: JP
authors.lastname: Souza
authors.affiliation: Laboratory of Microbial Ecology and Physiology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Jacques R
authors.initials: JR
authors.lastname: Nicoli
authors.affiliation: Neonatology, Hospital Sofia Feldman, Belo Horizonte, MG, Brazil.
authors.forename: Raquel L
authors.initials: RL
authors.lastname: Paula
authors.affiliation: AQUACEN, National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Gabriela B
authors.initials: GB
authors.lastname: Assis
authors.affiliation: AQUACEN, National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Henrique C
authors.initials: HC
authors.lastname: Figueiredo
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Laboratory of Biotherapeutic Agents, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. flaviano@icb.ufmg.br.|Laboratory of Microbial Ecology and Physiology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. flaviano@icb.ufmg.br.|Laboratório de Agentes Bioterapêuticos, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, C.P. 486, Pampulha - Campus UFMG, Belo Horizonte, MG, 31270-901, Brazil. flaviano@icb.ufmg.br.
authors.forename: Flaviano S
authors.initials: FS
authors.lastname: Martins
mesh_terms: D000328:Adult; D058491:Bacterial Load; D000069984:Bifidobacterium breve / Q000302:isolation & purification; D003126:Colostrum / Q000382:microbiology*; D005260:Female; D006801:Humans; D000069998:Lactobacillus gasseri / Q000302:isolation & purification; D008895:Milk, Human / Q000382:microbiology*; D019936:Probiotics; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D000070001:Streptococcus salivarius / Q000302:isolation & purification; D055815:Young Adult
keywords: Body mass index; Colostrum; Human milk; L. gasseri; Partum; Probiotics