title: Delivery of Morpholino Antisense Oligonucleotides to a Developing Ovine Conceptus via Luminal Injection into a Ligated Uterine Horn.
abstract: In vivo delivery of morpholino antisense oligonucleotides (MAO) directly into the uterine lumen of a peri-implantation period pregnant sheep is an effective technique for evaluation of gene products for conceptus development. The highly phagocytic conceptus is undergoing rapid morphological change, thereby the available MAO are readily consumed and delivered to developing cells. Here, we describe the method for preparation and surgical delivery of MAO-Endo-Porter complex to developing ovine conceptus on day 8 postmating. Also outlined are methods for posttreatment sample recovery on day 16 postmating.
authors.affiliation: Department of Animal Science, Texas A&M University, 2471 TAMUS, College Station, TX, 77843, USA.
authors.forename: Xiaoqui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Animal Science, Texas A&M University, 2471 TAMUS, College Station, TX, 77843, USA. kdunlap@tamu.edu.
authors.forename: Kathrin A
authors.initials: KA
authors.lastname: Dunlap
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D060172:Morpholinos / Q000008:administration & dosage* / Q000235:genetics*; D016376:Oligonucleotides, Antisense; D010455:Peptides; D014599:Uterus
chemical_list: C511217:Endo-Porter; D060172:Morpholinos; D016376:Oligonucleotides, Antisense; D010455:Peptides
keywords: Conceptus; Endo-Porter; Morpholino antisense oligonucleotide; Ovine; Peri-implantation; Uterine ligation