title: Unique high and homogenous surface expression of the transferrin receptor CD71 on murine plasmacytoid dendritic cells in different tissues.
abstract: Plasmacytoid dendritic cells (pDC) are of increasing interest in cancer vaccine development, but many functions of these highly specialized, multifaceted cells are poorly understood. The transferrin receptor CD71 has also been suggested to function as an antigen uptake receptor on professional antigen-presenting cells. In this study, we employed multiparameter flow cytometry to investigate CD71 expression on various leukocyte subsets, including DC subsets, granulocytes, macrophages, T and B lymphocytes, γδ T cells, and natural killer cells. Cells from various lymphoid and non-lymphoid murine tissues were analyzed using fluorochrome-conjugated monoclonal antibodies. High CD71 expression (90-100%) was observed, uniquely on pDC amongst the leukocyte populations examined, in both lymphoid and non-lymphoid tissues, including other DC subsets. In contrast, CD71 expression on non-tissue pDC, in the bone marrow and peripheral blood, was reduced. The cause and function of this high tissue pDC-selective CD71 expression remain to be examined.
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Lippitsch
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Chukovetskyi
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany.
authors.forename: Nelli
authors.initials: N
authors.lastname: Baal
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Bein
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, Biomedical Research Center Seltersberg (BFS), Schubertstrasse 81, D-35392 Giessen, Germany. Electronic address: Holger.Hackstein@immunologie.med.uni-giessen.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Hackstein
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism; D007370:Interferon Type I / Q000276:immunology / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D011990:Receptors, Transferrin / Q000096:biosynthesis* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C068126:CD71 antigen; D007370:Interferon Type I; D011990:Receptors, Transferrin
keywords: Dendritic cells; Plasmacytoid dendritic cells; Respiratory dendritic cells; Transferrin receptor CD71