title: Fermented milk containing Lactobacillus paracasei subsp. paracasei CNCM I-1518 reduces bacterial translocation in rats treated with carbon tetrachloride.
abstract: Probiotics can prevent pathological bacterial translocation by modulating intestinal microbiota and improving the gut barrier. The aim was to evaluate the effect of a fermented milk containing Lactobacillus paracasei subsp. paracasei CNCM I-1518 on bacterial translocation in rats with carbon tetrachloride (CCl4)-induced cirrhosis. Sprague-Dawley rats treated with CCl4were randomized into a probiotic group that received fermented milk containing Lactobacillus paracasei subsp. paracasei CNCM I-1518 in drinking water or a water group that received water only. Laparotomy was performed one week after ascites development. We evaluated bacterial translocation, intestinal microbiota, the intestinal barrier and cytokines in mesenteric lymph nodes and serum. Bacterial translocation decreased and gut dysbiosis improved in the probiotic group compared to the water group. The ileal β-defensin-1 concentration was higher and ileal malondialdehyde levels were lower in the probiotic group than in water group. There were no differences between groups in serum cytokines but TNF-α levels in mesenteric lymph nodes were lower in the probiotic group than in the water group. Fermented milk containing Lactobacillus paracasei subsp. paracasei CNCM I-1518 decreases bacterial translocation, gut dysbiosis and ileal oxidative damage and increases ileal β-defensin-1 expression in rats treated with CCl4, suggesting an improvement in the intestinal barrier integrity.
authors.affiliation: Department of Gastroenterology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.|Institut d´Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.|Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Sánchez
authors.affiliation: Institut d´Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.|Department of Immunology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Nieto
authors.affiliation: Institut d´Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.|Department of Immunology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Vidal
authors.affiliation: Digestive System Research Unit, Vall d'Hebron Research Institute, Barcelona, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Santiago
authors.affiliation: Digestive System Research Unit, Vall d'Hebron Research Institute, Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Martinez
authors.affiliation: Department of Pathology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Francesc J
authors.initials: FJ
authors.lastname: Sancho
authors.affiliation: Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.|Institut Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic de Barcelona, Barcelona, Spain.
authors.forename: Pau
authors.initials: P
authors.lastname: Sancho-Bru
authors.affiliation: Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.|Department of Microbiology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Mirelis
authors.affiliation: Danone Nutricia Research, Barcelona, Spain.
authors.forename: Helena
authors.initials: H
authors.lastname: Corominola
authors.affiliation: Institut d´Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.|Department of Immunology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Candido
authors.initials: C
authors.lastname: Juárez
authors.affiliation: Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.|Digestive System Research Unit, Vall d'Hebron Research Institute, Barcelona, Spain.
authors.forename: Chaysavanh
authors.initials: C
authors.lastname: Manichanh
authors.affiliation: Department of Gastroenterology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.|Institut d´Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.|Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Guarner
authors.affiliation: Department of Gastroenterology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.|Institut d´Investigacions Biomèdiques (IIB) Sant Pau, Barcelona, Spain.|Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain.|Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: German
authors.initials: G
authors.lastname: Soriano
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects / Q000502:physiology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D064806:Dysbiosis / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control; D005285:Fermentation; D000069196:Gastrointestinal Microbiome / Q000187:drug effects / Q000502:physiology*; D000069980:Lacticaseibacillus paracasei / Q000502:physiology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D019936:Probiotics / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D023083:beta-Defensins / Q000378:metabolism
chemical_list: C120555:Defb1 protein, mouse; D023083:beta-Defensins; D008315:Malondialdehyde; D002251:Carbon Tetrachloride