title: Effect of targeted silencing of IL-8 onin vitromigration and invasion of SKOV3 ovarian cancer cells.
abstract: The aim of the study was to determine whether interleukin-8 (IL-8) affects human SKOV3 ovarian cancer cell migration and invasion by targeting silencing of IL-8 expression. Silencing small-interfering RNA (siRNA) targetingIL-8gene was constructed to infect SKOV3 cells by lentiviral vector. The expression of IL-8 and p-nuclear factor (NF)-κB protein was detected by western blot analysis. The wound scratch and Transwell tests were used to assay the cell migration and invasiveness of SKOV3 cells infected with lentiviral vector targetingIL-8gene siRNA. The levels of IL-8 protein expressed by SKOV3 cells infected by lentiviral vector targetingIL-8gene siRNA decreased by 72.3%. IL-8 (50 ng/ml) increased the ability of SKOV3 cells to suppress cell migration (p<0.01). Cisplatin and silencing of IL-8 achieved the ability to inhibit SKOV3 cell invasion (p<0.01), and 100 ng/ml concentration of IL-8 enhanced the ability of SKOV3 invasion (p<0.01). Silencing of IL-8 to a certain extent reduced the expression of p-NF-κB proteins, but it was not statistically significant. In conclusion, silencing of IL-8 may inhibit the migration and invasion of SKOV3 cells, which may be independent of the p-NF-κB protein.
authors.affiliation: Department of Gynaecology and Obstetrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China; Xuzhou Clinical School, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Xuzhou Clinical School, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Gynaecology and Obstetrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Gynaecology and Obstetrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Gynaecology and Obstetrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tao
authors.affiliation: Department of Gynaecology and Obstetrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Gynaecology and Obstetrics, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China; Xuzhou Clinical School, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
keywords: cell invasion; cell migration; interleukin-8; ovarian cancer; p-nuclear factor-κB