title: Induced pluripotent stem-cell-derived cardiomyocytes: cardiac applications, opportunities, and challenges.
abstract: Chronic diseases are the primary cause of mortality worldwide, accounting for 67% of deaths. One of the major challenges in developing new treatments is the lack of understanding of the exact underlying biological and molecular mechanisms. Chronic cardiovascular diseases are the single most common cause of death worldwide, and sudden deaths due to cardiac arrhythmias account for approximately 50% of all such cases. Traditional genetic screening for genes involved in cardiac disorders is labourious and frequently fails to detect the mutation that explains or causes the disorder. However, when mutations are identified, human induced pluripotent stem cells (hiPSCs) derived from affected patients make it possible to address fundamental research questions directly relevant to human health. As such, hiPSC technology has recently been used to model human diseases and patient-specific hiPSC-derived cardiomyocytes (hiPSC-CMs) thus offer a unique opportunity to investigate potential disease-causing genetic variants in their natural environment. The purpose of this review is to present the current state of knowledge regarding hiPSC-CMs, including their potential, limitations, and challenges and to discuss future prospects.
authors.affiliation: a Centre de recherche de l'Institut universitaire en santé mentale de Québec, Quebec City, QC G1J 2G3, Canada.
authors.forename: Adrien
authors.initials: A
authors.lastname: Moreau
authors.affiliation: b Cardiovascular Research Program, VA New York Harbor Healthcare, Brooklyn, New York, USA.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Boutjdir
authors.affiliation: a Centre de recherche de l'Institut universitaire en santé mentale de Québec, Quebec City, QC G1J 2G3, Canada.|c Department of Medicine, Université Laval, Quebec City, QC G1K 7P4, Canada.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Chahine
mesh_terms: D000818:Animals; D002317:Cardiovascular Agents / Q000627:therapeutic use; D002318:Cardiovascular Diseases / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D020022:Genetic Predisposition to Disease; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism*; D009154:Mutation; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D010641:Phenotype
chemical_list: D002317:Cardiovascular Agents
keywords: NaV; arythmies cardiaques; canaux sodiques; cardiac arrhythmias; cardiac tissue; cardiomyocytes; electrophysiology; hIPSCs; sodium channels; tissu cardiaque; électrophysiologie