title: Integrating optical tweezers with up-converting luminescence: a non-amplification analytical platform for quantitative detection of microRNA-21 sequences.
abstract: We report a single-microsphere based imaging assay method by integrating up-converting luminescence with optical tweezers for detecting microRNA-21 sequences. This method achieves a competitive detection limit of 12 fM with good selectivity and no dedicated signal amplification designs.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Cao
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Chong-Yang
authors.initials: CY
authors.lastname: Song
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Chun-Miao
authors.initials: CM
authors.lastname: Xu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Xu-Yan
authors.initials: XY
authors.lastname: Ma
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn.
authors.forename: Hong-Wu
authors.identifier: http://orcid.org/0000-0002-2258-6992
authors.initials: HW
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques; D049449:Luminescence; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D008863:Microspheres; D052898:Optical Tweezers; D017423:Sequence Analysis, RNA
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs