title: Evaluation of GenoType NTM-DR Assay for Identification of Mycobacterium chimaera.
abstract: Identification of species within theMycobacterium aviumcomplex (MAC) is difficult, and most current diagnostic laboratory tests cannot distinguish between species included in the complex. Differentiation of species within the MAC is important, asMycobacterium chimaerahas recently emerged as a major cause of invasive cardiovascular infections following open heart surgery. A new commercial diagnostic assay, GenoType NTM-DR ver. 1.0, is intended to differentiate between three species within the MAC, namely,Mycobacterium avium,Mycobacterium intracellulare, andMycobacterium chimaeraIn this study, we investigated an archival collection of 173 MAC isolates using 16S rRNA and 16S-23S internal transcribed spacer (ITS) gene sequencing, and GenoType NTM-DR was evaluated for identifyingM. chimaeraand other species belonging to the MAC. Species identification of 157/173 (91%) isolates with the GenoType NTM-DR assay was in agreement with 16S rRNA and 16S-23S ITS gene sequencing results. Misidentification occurred with 16 isolates which belonged to four species included in the MAC that are rarely encountered in clinical specimens. Despite some limitations of this assay, GenoType NTM-DR had 100% specificity for identifyingM. chimaeraThis novel assay will enable diagnostic laboratories to differentiate species belonging to theMycobacterium aviumcomplex and to accurately identifyM. chimaeraIt can produce rapid results and is also more cost efficient than gene sequencing methods.
authors.affiliation: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland; Irish Mycobacteria Reference Laboratory, St. James's Hospital, Dublin, Ireland moks@tcd.ie.
authors.forename: Simone
authors.initials: S
authors.lastname: Mok
authors.affiliation: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland; Irish Mycobacteria Reference Laboratory, St. James's Hospital, Dublin, Ireland.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Rogers
authors.affiliation: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland; Irish Mycobacteria Reference Laboratory, St. James's Hospital, Dublin, Ireland.
authors.forename: Margaret
authors.initials: M
authors.lastname: Fitzgibbon
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S
keywords: GenoType NTM-DR; Mycobacterium avium complex; Mycobacterium chimaera; genotypic identification