title: Extracellular matrix mimicking scaffold promotes osteogenic stem cell differentiation: A new approach in osteoporosis research.
abstract: BACKGROUND
  Osteoporosis is a common metabolic disease, with mesenchymal stem cells discussed to play an important role in its pathomechanism. For in vitro osteoporosis studies, selection of adequate culture conditions is mandatory so as to preserve cell properties as far as possible. A suitable cell culture surface would ideally provide reproducible experimental conditions by resembling those in-vivo.
  
  
  OBJECTIVE
  Generating an improved growth surface for osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBMSCs).
  
  
  METHODS
  We modified electrospun gelatine meshes with hydroxyapatite nanopowder. The potential beneficial impact of the ensuing culture conditions were evaluated by cultivating and comparing the growth of cells from osteoporotic and non-osteoporotic donors on either hydroxyapatite-gelatine (HA) meshes, pure gelatine meshes, or 2D standard tissue culture surfaces.
  
  
  RESULTS
  After 21 days of differentiation, cells grown on pure or HA-gelatine meshes showed significantly higher mineralization levels compared to cells cultured in standard conditions. The amount of mineralization varied considerably in hBMSC cultures of individual patients but showed no significant difference between stem cells obtained from osteoporotic or non-osteoporotic donors.
  
  
  CONCLUSIONS
  Overall, these results indicate that the use of HA-gelatine meshes as growth surfaces may serve as a valuable tool for cultivation and differentiation of mesenchymal stem cells along the osteogenic lineage, facilitating future research on osteoporosis and related issues.
authors.affiliation: Department of Trauma Surgery, Medical University, Anichstrasse 35, Innsbruck, Austria.
authors.forename: C W I
authors.initials: CW
authors.lastname: Moll
authors.affiliation: Department of Therapeutic Radiology and Oncology, Medical University, Anichstrasse 35, Innsbruck, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Schmiedinger
authors.affiliation: Department of Vascular Surgery, Medical University, Anichstrasse 35, Innsbruck, Austria.
authors.forename: M A
authors.initials: MA
authors.lastname: Moll
authors.affiliation: Department of Therapeutic Radiology and Oncology, Medical University, Anichstrasse 35, Innsbruck, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Seppi
authors.affiliation: Division of Histology and Embryology, Medical University, Müllerstrasse 59, Innsbruck, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Pfaller
authors.affiliation: Division of Histology and Embryology, Medical University, Müllerstrasse 59, Innsbruck, Austria.
authors.forename: M W
authors.initials: MW
authors.lastname: Hess
authors.affiliation: Division of Histology and Embryology, Medical University, Müllerstrasse 59, Innsbruck, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Gutleben
authors.affiliation: Department of Trauma Surgery, Medical University, Anichstrasse 35, Innsbruck, Austria.
authors.forename: R A
authors.initials: RA
authors.lastname: Lindtner
authors.affiliation: Department of Trauma Surgery, Medical University, Anichstrasse 35, Innsbruck, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Blauth
authors.affiliation: OROBOROS® INSTRUMENTS Corporation (GmbH), Schöpfstrasse 18, Innsbruck, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Krumschnabel
authors.affiliation: Department of Trauma Surgery, Medical University, Anichstrasse 35, Innsbruck, Austria.|MED-EL Medical Electronics (GmbH), Fürstenweg 77a, Innsbruck, Austria.
authors.forename: H L
authors.initials: HL
authors.lastname: Ebner
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin; D017886:Durapatite
keywords: Osteoporosis; differentiation; electrospun gelatine mesh; hydroxyapatite; mesenchymal stem cells