title: Bisphenol A Inhibits Cell Proliferation and Reduces the Motile Potential of Murine LM8 Osteosarcoma Cells.
abstract: The aim of this study was to examine the effect of bisphenol A (BPA) on the proliferation and motility potential of murine LM8 osteosarcoma cells.
  
  
  
  LM8 cells were treated for 3 days with or without 80 μM BPA. The effect of BPA on cell proliferation was determined by DNA measurement in the cultures and 5-bromo-2'-deoxyuridine (BrdU) incorporation study. Ethanol-fixed cells were stained with hematoxylin-eosin (H&E) to visualize cell morphology. Cell motility was assayed using inserts with uncoated membranes in invasion chambers. Expression of cell division cycle 42 (CDC42) was determined by immunofluorescence staining and western blotting.
  
  
  
  BPA reduced the DNA content of cultures and the number of BrdU-positive cells. BPA induced a change in morphology from cuboidal with multiple filopodia on the cell surface to spindle-shaped with a smooth cell surface. BPA-treated cells expressed less CDC42 and were less motile than untreated cells.
  
  
  
  BPA inhibited DNA replication and cell proliferation. BPA inhibited filopodia formation and motile potential by inhibiting CDC42 expression in LM8 cells.
authors.affiliation: Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Toon, Japan teruteru@m.ehime-u.ac.jp.
authors.forename: Teruki
authors.initials: T
authors.lastname: Kidani
authors.affiliation: Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Tobe, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Yasuda
authors.affiliation: Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Toon, Japan.
authors.forename: Joji
authors.initials: J
authors.lastname: Miyawaki
authors.affiliation: Department of Molecular Medicine for Pathogenesis, Ehime University Graduate School of Medicine, Toon, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oshima
authors.affiliation: Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Toon, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Miura
authors.affiliation: Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Tobe, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masuno
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D051379:Mice; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D010636:Phenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D012333:RNA, Messenger; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: CDC42 expression; Murine LM8 osteosarcoma cells; bisphenol A; cell motility; cell proliferation; filopodia formation