title: Determination of O:4 antigen-antibody affinity level in O:5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium.
abstract: Salmonella enterica serovar Typhimurium (S. Typhimurium) has two serological variants: one that expresses the O:5 antigen (1,4,5,12:i:1,2) and one that lacks O:5 antigen (1,4,12:i:1,2). For serotyping, S. Typhimurium is agglutinated by diagnostic O:4 antigen serum. This study was carried out to compare the antigen-antibody affinity of O:4 antigen in S. Typhimurium χ3306 O:5-positive and S. Typhimurium χ3306 O:5-negative strains. The affinity of O:4 antigen with O:4 antigen serum was found to be stronger in the O:5-negative strains compared to O:5-positive strains. Next, we investigated the antigen-antibody affinity of O:4 antigen with O:4 antigen serum in field strains of S. Typhimurium, which showed the same tendency in affinity as seen with S. Typhimurium χ3306 O:5-positive and negative strains. This study suggests that the presence or absence of O:5 antigen causes differences in O:4 agglutination reactions with different field strains of S. Typhimurium.
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nakai
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Ito
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Swarmistha Devi
authors.initials: SD
authors.lastname: Aribam
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Marta
authors.initials: M
authors.lastname: Elsheimer-Matulova
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shiraiwa
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Stevens M B
authors.initials: SMB
authors.lastname: Kisaka
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hikono
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Nishikawa
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Akiba
authors.affiliation: Department of Biosciences, College of Science and Engineering, Kanto Gakuin University, 1-50-1, Mutsuura-Higashi, Kanazawa-ku, Yokohama 236-8501, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawahara
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimoji
authors.affiliation: National Institute of Animal Health, NARO, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Eguchi
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000276:immunology*; D000915:Antibody Affinity; D004269:DNA, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D019081:O Antigens / Q000737:chemistry / Q000276:immunology*; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D065288:Serogroup; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D019081:O Antigens
keywords: O:5 antigen; Salmonella enterica serovar Typhimurium; microagglutination test; serodiagnosis