title: Pyranocarbazoles from Murraya koenigii (L.) Spreng. as antimicrobial agents.
abstract: The bioassay guided fractionation of methanolic extract of Murraya koenigii (L.) Spreng. leaves resulted in the isolation of seven pyranocarbazoles. These were evaluated against four bacterial strains and ten Candida sp. including two matched pair of fluconazole sensitive/resistant clinical isolates. Out of seven, three i.e. Koenine (mk279), Koenigine (mk309) and Mahanine (mk347) exhibited significant antibacterial activity MIC90 3.12-12.5 μg/mL against bacterial strains Streptococcus aureus and Klebsiella pneumonia compared with standard drug Kanamycin MIC90 12.5 μg/mL. However, only mk309 was found active against variety of Candida species MIC90 12.5-100 μg/mL. It was observed that hydroxylation at C-6 and C-7 positions in the studied pyranocarbazoles activate the bioactivity. Simultaneously, decrease in Log P value compares with -H and -O-CH3 substituted derivatives. The study is focused on selective antifungal and antibacterial activity of pyranocarbazoles on bacterial strains S. aureus, K. pneumonia and variety of Candida species with structure activity relationship observations.
authors.affiliation: a Sophisticated Analytical Instrument Facility , CSIR-Central Drug Research Institute , Lucknow , India.
authors.forename: Trapti
authors.initials: T
authors.lastname: Joshi
authors.affiliation: b Molecular and Structural Biology Division , CSIR-Central Drug Research Institute , Lucknow , India.|e Academy of Scientific and Innovative Research , India.
authors.forename: Tushar
authors.initials: T
authors.lastname: Jain
authors.affiliation: a Sophisticated Analytical Instrument Facility , CSIR-Central Drug Research Institute , Lucknow , India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Mahar
authors.affiliation: c Botany Division , CSIR-Central Drug Research Institute , Lucknow , India.|e Academy of Scientific and Innovative Research , India.
authors.forename: Sumit K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: c Botany Division , CSIR-Central Drug Research Institute , Lucknow , India.
authors.forename: Piush
authors.initials: P
authors.lastname: Srivastava
authors.affiliation: a Sophisticated Analytical Instrument Facility , CSIR-Central Drug Research Institute , Lucknow , India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Shukla
authors.affiliation: c Botany Division , CSIR-Central Drug Research Institute , Lucknow , India.|e Academy of Scientific and Innovative Research , India.
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Mishra
authors.affiliation: d Division of Pharmacokinetics and Metabolism , CSIR-Central Drug Research Institute , Lucknow , India.
authors.forename: R S
authors.initials: RS
authors.lastname: Bhatta
authors.affiliation: b Molecular and Structural Biology Division , CSIR-Central Drug Research Institute , Lucknow , India.|e Academy of Scientific and Innovative Research , India.
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Banerjee
authors.affiliation: a Sophisticated Analytical Instrument Facility , CSIR-Central Drug Research Institute , Lucknow , India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Kanojiya
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002227:Carbazoles / Q000737:chemistry / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D032101:Murraya / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002227:Carbazoles; D010936:Plant Extracts; C465290:mahanine
keywords: Klebsiella pneumonia; Pyranocarbazoles; Streptococcus aureus; antibacterial; antifungal; carbazole alkaloids