title: Green synthesis of isomaltulose from cane molasses by Bacillus subtilis WB800-pHA01-palI in a biologic membrane reactor.
abstract: A green process and environmentally benign process is highly desirable in the development of enzymatic catalysis. In this work, the shuttle plasmid pHA01 was constructed and the sucrose isomerase (SIase) was expressed in Bacillus subtilis WB800. The optimal nitrogen and carbon sources for SIase expression were yeast extract (15g/L) and un-pretreated cane molasses (UCM, 20g/L), respectively. After the UCM fed, the whole cell activity reached 5.2U/mL in a 7.5L fermentor. Optimum catalytic temperature and pH of whole cell were 35°C and 5.5, respectively. Although the biologic membrane reactor (BMR) system consecutively worked for 12 batches, the sucrose conversion remained higher than 90%, indicating the BMR system had a greater operational stability. Furthermore, isomaltulose production using the BMR system with low-cost cane molasses as its substrate not only reduces the production cost and mediates environmental pollution, but also solves the genetic background problem of the non-food-grade strains.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, 5 Xin Mofan Road, Nanjing 210009, PR China; College of Food Science and Light Industry, 30 South Puzhu Road, Nanjing Tech University, Nanjing 211816, PR China; College of Biological and Pharmaceutical Engineering, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, PR China.
authors.forename: Lingtian
authors.initials: L
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, 5 Xin Mofan Road, Nanjing 210009, PR China; College of Biological and Pharmaceutical Engineering, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, 5 Xin Mofan Road, Nanjing 210009, PR China; College of Food Science and Light Industry, 30 South Puzhu Road, Nanjing Tech University, Nanjing 211816, PR China; College of Biological and Pharmaceutical Engineering, Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, PR China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Qiu
authors.affiliation: College of Food Science and Light Industry, 30 South Puzhu Road, Nanjing Tech University, Nanjing 211816, PR China.
authors.forename: Chuanxue
authors.initials: C
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, 5 Xin Mofan Road, Nanjing 210009, PR China; College of Food Science and Light Industry, 30 South Puzhu Road, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: lisha@njtech.edu.cn.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, 5 Xin Mofan Road, Nanjing 210009, PR China; College of Food Science and Light Industry, 30 South Puzhu Road, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: xuh@njtech.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001688:Biological Products / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism; D019149:Bioreactors; D055772:Green Chemistry Technology / Q000379:methods*; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism; D008965:Molasses / Q000032:analysis; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: D001688:Biological Products; D007534:Isomaltose; C008189:isomaltulose
keywords: Biologic membrane reactor; Cane molasses; Food safety; Heterologous expression; Isomaltulose; Isomaltulose (PubChem CID: 439559); Sucrose (PubChem CID: 5988); Sucrose isomerase; Trehalulose (PubChem CID: 162104)