title: Amplification and up-regulation of MIR30D was associated with disease progression of cervical squamous cell carcinomas.
abstract: Cervical squamous cell carcinoma (CSCC) is the most frequent type among cervical cancers. Although the altered miRNA miR-30d expression and the amplified chromosome locus of MIR30D, 8q24, have been reported in somatic cancers, the definitive functional impact of such region especially in CSCC remains under-investigated.
  
  
  
  One hundred thirty-six cases of CSCC tissues and matched adjacent normal ovarian epithelial tissues were assessed in this study. FISH and qPCR were performed to detect the copy number and microRNA expression of MIR30D gene in the collected samples. In in-vitro study, proliferation of CSCC cells were analyzed using WST-1 assay and invasion abilities of CSCC cells were evaluated by transwell assay. In-vivo study using a model of nude mice bearing tumor was also performed.
  
  
  
  Copy number gains of MIR30D were detected in 22.8% (31 out of 136) of CSCC samples. Copy number of MIR30D was positively correlated with tumor progression. CSCCs with lymph node metastases (LNM) also showed more frequencies (36.4%) of MIR30D amplification than those without LNM (18.4%, p < 0.05). CSCCs with increased copy number of MIR30D also showed a positive correlation with miR-30d up-regulation. Inhibition of miR-30d in CSCC cells led to impaired tumor growth and migration.
  
  
  
  Copy number amplifications of MIR30D gene and enhanced expression of miR-30d were positively correlated with tumor progression in CSCCs, indicating miR-30d might play an oncomiric role in the progression of CSCC.
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: You
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of Medical Oncology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China. wangshubin2013@163.com.
authors.forename: Shubin
authors.initials: S
authors.lastname: Wang
authors.affiliation: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, 518036, China. fangzy796@163.com.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Fang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D056915:DNA Copy Number Variations / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D015854:Up-Regulation / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D035683:MicroRNAs
keywords: Cervical squamous cell carcinoma; Copy number variation; Gene expression; MIR30D; miR-30d