title: Molecular Identification, Enzyme Assay, and Metabolic Profiling ofTrichodermaspp.
abstract: The goal of this study was to identify and characterize selectedTrichodermaisolates by metabolic profiling and enzyme assay for evaluation of their potential as biocontrol agents against plant pathogens.Trichodermaisolates were obtained from the Rural Development Administration Genebank Information Center (Wanju, Republic of Korea). ElevenTrichodermaisolates were re-identified using ribosomal DNA internal transcribed spacer (ITS) regions. ITS sequence results showed new identification ofTrichodermaisolates. In addition, metabolic profiling of the ethyl acetate extracts of the liquid cultures of fiveTrichodermaisolates that showed the best anti-Phytophthoraactivities was conducted using gas chromatography-mass spectrometry. Metabolic profiling revealed thatTrichodermaisolates shared common metabolites with well-known antifungal activities. Enzyme assays indicated strong cell walldegrading enzyme activities ofTrichodermaisolates. Overall, our results indicated that the selectedTrichodermaisolates have great potential for use as biocontrol agents against plant pathogens.
authors.affiliation: Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Soo-Jung
authors.initials: SJ
authors.lastname: Bae
authors.affiliation: Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
authors.affiliation: Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Junhyun
authors.initials: J
authors.lastname: Jeon
authors.affiliation: Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Hanhong
authors.initials: H
authors.lastname: Bae
mesh_terms: D000898:Antibiosis; D002473:Cell Wall / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D057075:Enzyme Assays; D008401:Gas Chromatography-Mass Spectrometry; D055432:Metabolomics; D010572:Pest Control, Biological / Q000379:methods; D010838:Phytophthora; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Trichoderma; cell wall degradation enzyme activity; metabolic profiling; molecular identification