title: Low-dose fixed-target serial synchrotron crystallography.
abstract: The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption.
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Robin L
authors.initials: RL
authors.lastname: Owen
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Danny
authors.initials: D
authors.lastname: Axford
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.
authors.forename: Darren A
authors.initials: DA
authors.lastname: Sherrell
authors.affiliation: Department of Biochemistry, University of Toronto, King's College Circle, Toronto, Ontario M5S 1A8, Canada.
authors.forename: Anling
authors.initials: A
authors.lastname: Kuo
authors.affiliation: Department of Biochemistry, University of Toronto, King's College Circle, Toronto, Ontario M5S 1A8, Canada.
authors.forename: Oliver P
authors.initials: OP
authors.lastname: Ernst
authors.affiliation: Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
authors.forename: Eike C
authors.initials: EC
authors.lastname: Schulz
authors.affiliation: Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
authors.forename: R J Dwayne
authors.initials: RJ
authors.lastname: Miller
authors.affiliation: Department of Biochemistry, University of Toronto, King's College Circle, Toronto, Ontario M5S 1A8, Canada.
authors.forename: Henrike M
authors.initials: HM
authors.lastname: Mueller-Werkmeister
mesh_terms: D000818:Animals; D003460:Crystallization / Q000295:instrumentation; D018360:Crystallography, X-Ray / Q000295:instrumentation*; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry*; D050661:Sperm Whale; D017356:Synchrotrons
chemical_list: D009211:Myoglobin
keywords: crystallography on a chip; fixed target; low dose; macromolecular crystallography; room-temperature crystallography; serial synchrotron crystallography