title: Telomere length profiles in primary human peritoneal mesothelial cells are consistent with senescence.
abstract: Mesothelial cell (MC) senescence contributes to malignancy and tissue fibrosis. The role of telomere erosion in MC senescence remains controversial, with evidence for both telomere-dependent and telomere-independent mechanisms reported. Single telomere length analysis revealed considerable telomere length heterogeneity in freshly isolated human peritoneal MCs, reflecting a heterogeneous proliferative history and providing high-resolution evidence for telomere-dependent senescence. By contrast the attenuated replicative lifespan, lack of telomere erosion and induction of p16 expression in in vitro-aged cells was consistent with stress-induced senescence. Given the potential pathophysiological impact of senescence in mesothelial tissues, high-resolution MC telomere length analysis may provide clinically useful information.
authors.affiliation: Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: LopezAntonM@cf.ac.uk.
authors.forename: Melisa
authors.initials: M
authors.lastname: Lopez-Anton
authors.affiliation: Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: arudolf@umed.poznan.pl.
authors.forename: András
authors.initials: A
authors.lastname: Rudolf
authors.affiliation: Division of Cancer and Genetics, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: bairddm@cf.ac.uk.
authors.forename: Duncan M
authors.initials: DM
authors.lastname: Baird
authors.affiliation: Division of Cancer and Genetics, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: rogerl@cf.ac.uk.
authors.forename: Laureline
authors.initials: L
authors.lastname: Roger
authors.affiliation: Division of Cancer and Genetics, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Electronic address: jonesr47@cf.ac.uk.
authors.forename: Rhiannon E
authors.initials: RE
authors.lastname: Jones
authors.affiliation: Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: jwitow@ump.edu.pl.
authors.forename: Janusz
authors.initials: J
authors.lastname: Witowski
authors.affiliation: Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Museum Place, Cardiff CF10 3BG, UK. Electronic address: fraserdj@cf.ac.uk.
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Fraser
authors.affiliation: Wales Kidney Research Unit, Division of Infection and Immunity, School of Medicine, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Museum Place, Cardiff CF10 3BG, UK. Electronic address: bowent@cf.ac.uk.
authors.forename: Timothy
authors.initials: T
authors.lastname: Bowen
mesh_terms: D016922:Cellular Senescence / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*; D059505:Telomere Homeostasis / Q000502:physiology*
keywords: Mesothelium; Premature senescence; Single telomere length analysis