title: OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese.
abstract: An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in granulosa cells of geese was carried out in the current study. The effect of OAZ1 knockdown on polyamine metabolism, cell proliferation, apoptosis, and hormone receptor transcription of primary granulosa cells in geese was measured. The viability of granulosa cells transfected with the shRNA OAZ1 at 48 h was significantly higher than the control (p<0.05). The level of putrescine and spermidine in granulosa cells down-regulating OAZ1 was 7.04- and 2.11- fold higher compared with the control, respectively (p<0.05). The CCND1, SMAD1, and BCL-2 mRNA expression levels in granulosa cells down-regulating OAZ1 were each significantly higher than the control, respectively (p<0.05), whereas the PCNA and CASPASE 3 expression levels were significantly lower than the control (p<0.05). The estradiol concentration, ER and LHR mRNA expression levels were significantly lower in granulosa cells down-regulating OAZ1 compared with the control (p<0.05). Taken together, our results indicated that OAZ1 knockdown elevated the putrescine and spermidine contents and enhanced granulosa cell viability and inhibited ER and LHR transcriptions of granulosa cells in geese.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.|Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
authors.forename: Bo
authors.identifier: http://orcid.org/0000-0003-2811-0642
authors.initials: B
authors.lastname: Kang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.|Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Yi
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005777:Geese; D006107:Granulosa Cells / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051898:Smad1 Protein; C031017:ornithine decarboxylase antizyme; D019938:Cyclin D1; D053148:Caspase 3