title: Novel benzanthrone probes for membrane and protein studies.
abstract: The applicability of a series of novel benzanthrone dyes to monitoring the changes in physicochemical properties of lipid bilayer and to differentiating between the native and aggregated protein states has been evaluated. Based on the quantitative parameters of the dye-membrane and dye-protein binding derived from the fluorimetric titration data, the most prospective membrane probes and amyloid tracers have been selected from the group of examined compounds. Analysis of the red edge excitation shifts of the membrane- and amyloid-bound dyes provided information on the properties of benzanthrone binding sites within the lipid and protein matrixes. To understand how amyloid specificity of benzanthrones correlates with their structure, quantitative structure activity relationship (QSAR) analysis was performed involving a range of quantum chemical molecular descriptors. A statistically significant model was obtained for predicting the sensitivity of novel benzanthrone dyes to amyloid fibrils.
authors.affiliation: Department of Nuclear and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv 61022, Ukraine. Author to whom any correspondence should be addressed: Department of Nuclear and Medical Physics, 12-191 Staroshyskivska Str., Kharkiv 61070, Ukraine.
authors.forename: Olga
authors.initials: O
authors.lastname: Ryzhova
authors.forename: Kateryna
authors.initials: K
authors.lastname: Vus
authors.forename: Valeriya
authors.initials: V
authors.lastname: Trusova
authors.forename: Elena
authors.initials: E
authors.lastname: Kirilova
authors.forename: Georgiy
authors.initials: G
authors.lastname: Kirilov
authors.forename: Galyna
authors.initials: G
authors.lastname: Gorbenko
authors.forename: Paavo
authors.initials: P
authors.lastname: Kinnunen
mesh_terms: D000682:Amyloid; D001551:Benz(a)Anthracenes / Q000737:chemistry*; D001665:Binding Sites; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D015335:Molecular Probes; D011446:Prospective Studies; D011485:Protein Binding; D021281:Quantitative Structure-Activity Relationship; D013050:Spectrometry, Fluorescence
chemical_list: D000682:Amyloid; D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D015335:Molecular Probes; C012768:benzanthrone