title: Chitosan/gelatin/platelet gel enriched by a combination of hydroxyapatite and beta-tricalcium phosphate in healing of a radial bone defect model in rat.
abstract: This study compared the regeneration potentials of the hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP) alone or in combination with a HA:TCP ratio of 30:70 in the critical-sized radial bone defects of rats. Bilateral 60 radial bone defects created were randomly divided into six equal groups (n=10 defects/group) including autograft, untreated or defect, chitosan-gelatin-platelet gel (CGP), CGP-HA, CGP-TCP and CGP-HA/TCP. The defects were evaluated by radiography, morphology, histopathology, histomorphometry, CT scan, scanning electron microscopy and biomechanical testing after eight weeks. Compared with the untreated and CGP-HA groups, the CGP and CGP-HA/TCP groups showed significantly higher new bone formation, bone volume, and mechanical properties. The CGP-HA and CGP-TCP scaffolds showed low biodegradability, whereas the CGP scaffolds were completely degraded. Osteoconductivity and osteoinductivity of the CGP and CGP-HA/TCP scaffolds were superior to the CGP-HA ones. The untreated and CGP-HA groups repaired mostly through fibrosis, while there were evidence of higher bone formation in the autograft, CGP and CGP-HA/TCP groups. In conclusion, addition of HA or β-TCP alone into the CGP scaffolds impaired bone regeneration, while bone regeneration with the CGP and CGP-HA/TCP scaffolds was comparable with the autograft. Therefore, the CGP-HA/TCP scaffold can be a possible option to substitute the autologous bone grafting.
authors.affiliation: Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: Oryan1215@gmail.com.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Oryan
authors.affiliation: Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Soodeh
authors.initials: S
authors.lastname: Alidadi
authors.affiliation: Department of Surgery and Radiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Bigham-Sadegh
authors.affiliation: Department of Surgery and Radiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Abdolhamid
authors.initials: A
authors.lastname: Meimandi-Parizi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001696:Biomechanical Phenomena; D001792:Blood Platelets / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D011884:Radius / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D014457:Ulna / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D005780:Gelatin; D048271:Chitosan; D017886:Durapatite
keywords: Beta-tricalcium phosphate; Bone healing and regeneration; Hydroxyapatite; Platelet-rich plasma; Radius