title: Pancreatic neuroendocrine carcinoma with exocrine differentiation in a young cat.
abstract: A 35-mo-old spayed female mixed-breed cat with continuous vomiting, emaciation, and abdominal distention for 2 wk was presented to a private veterinary clinic for evaluation. At 71 d after the initial visit, the cat died with anemia, jaundice, and hypoalbuminemia, and was subjected to autopsy. Grossly, numerous firm masses, 0.5-2.5 cm diameter, were randomly located in the left lobe of the pancreas. Histologic examination revealed that the pancreatic mass consisted of 2 tumor cell types: mostly small round cells with a minority of epithelial cells. The small cells were arranged in nests of various sizes, which were separated by thin fibrous stroma, and had small, round, hyperchromatic nuclei, scant cytoplasm containing argyrophilic granules, and often formed rosettes. The epithelial cells formed luminal structures. Metastases were observed in the liver, greater omentum, and pancreatic, gastric, pulmonary, and mediastinal lymph nodes. Immunohistochemical examination revealed that the small cells were positive for vimentin, neuron-specific enolase, chromogranin A, cytokeratin (CK) AE1/AE3, and trypsin, whereas the epithelial cells were positive for AE1/AE3, trypsin, CK19, and nestin. Ultrastructurally, the small cells contained abundant electron-dense granules, ~200 nm diameter, whereas the epithelial cells had apical microvilli and numerous zymogen granules, ~300 nm diameter. These findings indicated that the tumor was a pancreatic neuroendocrine carcinoma with exocrine differentiation and systemic metastases.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Michishita
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Takagi
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Takuya E
authors.initials: TE
authors.lastname: Kishimoto
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Rei
authors.initials: R
authors.lastname: Nakahira
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nogami
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yoshimura
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hatakeyama
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ochiai
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine (Michishita, Takagi, Kishimoto, Nakahira, Nogami, Takahashi), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Applied Science, School of Veterinary Nursing and Technology (Yoshimura), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Comparative Cellular Biology, School of Veterinary Medicine (Hatakeyama), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Veterinary Nursing, School of Veterinary Nursing and Technology (Azakami), Nippon Veterinary and Life Science University, Tokyo, Japan.|Department of Basic Science, School of Veterinary Nursing and Technology (Ochiai), Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Kimimasa
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D018278:Carcinoma, Neuroendocrine / Q000175:diagnosis / Q000556:secondary / Q000662:veterinary*; D002371:Cat Diseases / Q000175:diagnosis* / Q000473:pathology; D002415:Cats; D003937:Diagnosis, Differential; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*
keywords: Cats; metastasis; neuroendocrine carcinoma; pancreas