title: Impact of single nucleotide polymorphism in chemical metabolizing genes and exposure to wood smoke on risk of cervical cancer in North-Indian women.
abstract: OBJECTIVE
  In the present study, we investigated the hypothesis whether exposure to wood smoke increases the risk of cervical cancer (CC) in North-Indian women who inherit different polymorphic forms of chemical metabolizing genes (GSTM1, GSTT1, GSTP1 and CYP1A1).
  
  
  METHODS
  One hundred fifty histologically confirmed CC patients and equal number of cancer-free age and ethnicity matched controls were genotyped for genetic polymorphism in chemical metabolizing genes by using polymerase chain reaction/restriction fragment length polymorphism method. The association of the different genotypes and exposure to wood smoke with the risk of CC in North-Indian women was estimated by doing statistical analysis using Statistical Package for the Social Science.
  
  
  RESULTS
  It was observed that the variant genotypes of GSTM1, GSTT1, GSTP1 and CYP1A1 did not significantly increase the risk of CC. However, statistically significant increased risk (odds ratio 3.6; 95% confidence interval, 1.34-9.78; p = 0.008) was observed for women who used wood for cooking and had GSTM1 (null) genotype.
  
  
  CONCLUSIONS
  The present study suggests that genetic differences in the metabolism of wood smoke carcinogens, particularly by GSTM1, may increase the risk of CC.
authors.affiliation: Department of Biotechnology, Sri Guru Gobind Singh College, Chandigarh 160019, India.
authors.forename: K
authors.initials: K
authors.lastname: Satinder
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh 160014, India.
authors.forename: R C
authors.initials: RC
authors.lastname: Sobti
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh 160014, India.
authors.forename: K
authors.initials: K
authors.lastname: Pushpinder
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007194:India; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018570:Risk Assessment / Q000379:methods / Q000706:statistics & numerical data; D012307:Risk Factors; D012906:Smoke / Q000009:adverse effects*; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D014934:Wood / Q000737:chemistry*
chemical_list: D019363:Cytochrome P-450 CYP1A1; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D012906:Smoke; C569463:CYP1A1 protein, human; C413545:glutathione S-transferase T1