title: Plasma protein binding monitoring of therapeutic drugs in patients using single set of hollow fiber centrifugal ultrafiltration.
abstract: OBJECTIVE
  Plasma protein binding (PPB), as a significant influenced factor of pharmacokinetic and pharmacodynamic properties of a medicine, is a suitable index for therapeutic drug monitoring (TDM) strategies. A suitable measurement technique of PPB of patients is in urgent need and attracts many analysts' attention. Results & methodology: In this study, a novel method was proposed to analyze free drug concentration and total drug concentration (Ct) successively in one unit with a sample. All RSDs were less than 3%. The absolute recovery of Ctranged from 98.1 to 101.2%.
  
  
  CONCLUSIONS
  It is extremely valuable to consider PPB as an important index for TDM, perfecting information of medication, reflecting the disease condition more comprehensively, providing assistance for doctors to adjust the dose regimen. The proposed technique, convenience, accuracy and without the influence of plasma condition, provides a feasible method to monitor PPB of various patients, facilitating the popularization of monitoring PPB in TDM.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, The Central Hospital of China National Petroleum, Corporation, langfang, Hebei Province 050000, China.
authors.forename: Cen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Du
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Jing-Lin
authors.initials: JL
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, The Central Hospital of China National Petroleum, Corporation, langfang, Hebei Province 050000, China.
authors.forename: Ke-Xin
authors.initials: KX
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, The Central Hospital of China National Petroleum, Corporation, langfang, Hebei Province 050000, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000628:Aminophylline / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D001993:Bronchodilator Agents / Q000378:metabolism; D002498:Centrifugation / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000378:metabolism*; D011485:Protein Binding; D012140:Respiratory Tract Diseases / Q000188:drug therapy* / Q000378:metabolism; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*
chemical_list: D001798:Blood Proteins; D001993:Bronchodilator Agents; D000628:Aminophylline
keywords: aminophylline; hollow fiber ultrafiltration; plasma protein binding; release agent; therapeutic drug monitoring