title: Genetic characterization of human herpesvirus type 1: Full-length genome sequence of strain obtained from an encephalitis case from India.
abstract: OBJECTIVE
  Human herpes simplex virus 1 (HSV-1) is the most common cause of sporadic encephalitis in humans that contributes to >10 per cent of the encephalitis cases occurring worldwide. Availability of limited full genome sequences from a small number of isolates resulted in poor understanding of host and viral factors responsible for variable clinical outcome. In this study genetic relationship, extent and source of recombination using full-length genome sequence derived from a newly isolated HSV-1 isolate was studied in comparison with those sampled from patients with varied clinical outcome.
  
  
  METHODS
  Full genome sequence of HSV-1 isolated from cerebrospinal fluid (CSF) of a patient with acute encephalitis syndrome (AES) by inoculation in baby hamster kidney-21 (BHK-21) cells was determined using next-generation sequencing (NGS) technology. Phylogenetic analysis of the newly generated sequence in comparison with 33 additional full-length genomes defined genetic relationship with worldwide distributed strains. The bootscan and similarity plot analysis defined recombination crossovers and similarities between newly isolated Indian HSV-1 with six Asian and a total of 34 worldwide isolated strains.
  
  
  RESULTS
  Mapping of 376,332 reads amplified from HSV-1 DNA by NGS generated full-length genome of 151,024 bp from newly isolated Indian HSV-1. Phylogenetic analysis classified worldwide distributed strains into three major evolutionary lineages correlating to their geographic distribution. Lineage 1 containing strains were isolated from America and Europe; lineage 2 contained all the strains from Asian countries along with the North American KOS and RE strains whereas the South African isolates were distributed into two groups under lineage 3. Recombination analysis confirmed events of recombination in Indian HSV-1 genome resulting from mixing of different strains evolved in Asian countries.
  
  
  CONCLUSIONS
  Our results showed that the full-length genome sequence generated from an Indian HSV-1 isolate shared close genetic relationship with the American KOS and Chinese CR38 strains which belonged to the Asian genetic lineage. Recombination analysis of Indian isolate demonstrated multiple recombination crossover points throughout the genome. This full-length genome sequence amplified from the Indian isolate would be helpful to study HSV evolution, genetic basis of differential pathogenesis, host-virus interactions and viral factors contributing towards differential clinical outcome in human infections.
authors.affiliation: National Institute of Virology (ICMR), Pune, India.
authors.forename: Vijay P
authors.initials: VP
authors.lastname: Bondre
authors.affiliation: National Institute of Virology (ICMR), Pune, India.
authors.forename: Vasudha
authors.initials: V
authors.lastname: Sankararaman
authors.affiliation: National Institute of Virology (ICMR), Pune, India.
authors.forename: Vijaysinh
authors.initials: V
authors.lastname: Andhare
authors.affiliation: National Institute of Virology (ICMR), Pune, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Tupekar
authors.affiliation: National Institute of Virology (ICMR), Pune, India.
authors.forename: Gajanan N
authors.initials: GN
authors.lastname: Sapkal
mesh_terms: D004660:Encephalitis / Q000235:genetics* / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000472:pathogenicity; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007194:India; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA