title: In Vivo Imaging of Budding Yeast Meiosis.
abstract: Tracking biological events in living cells provides kinetic information about biological processes that can be missed in more traditional methods using fixed samples at designated time intervals. Here we describe a methodology for in vivo fluorescence microscopy of yeast cells undergoing meiosis. This method allows tracking of individual cells over extended periods of time through every stage of the meiotic transformation while minimizing phototoxicity and sustaining conditions that support meiotic growth.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Center of Reproductive Sciences, University of California, San Francisco, CA, 94143, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Pollard
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Center of Reproductive Sciences, University of California, San Francisco, CA, 94143, USA. jennifer.fung@ucsf.edu.|Fung Lab, University of California, San Francisco, GH N416, 600 16th St., San Francisco, CA, 94158, USA. jennifer.fung@ucsf.edu.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Fung
mesh_terms: D008540:Meiosis; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D004718:Saccharomycetales / Q000235:genetics / Q000502:physiology*
keywords: In vivo fluorescence microscopy; Live imaging; Meiosis; Yeast