title: Antibacterial function of herbal extracts on growth, survival and immunoprotection in the black tiger shrimp Penaeus monodon.
abstract: This study examined the effects of an herbal extract composed of nine herbs i.e Aloe vera, Andrographis pariculata, Annona squamosa, Azadirachta indica, Citrus aurantifolia, Coriandrum sativum, Ocimum sanctum, Ollium cepa and Psidium guajava on growth, survival rate and immunoprotection against pathogenic Vibrio harveyi in the tiger shrimp Penaeus monodon. The petroleum ether, methanol and N-hexen extracts of different herbal plants were selected, processed and thoroughly mixed in equal proportions and added to the shrimp diets at a concentration of 1.0, 2.5 and 5.0 mL kg-1. After 60 days of feeding, shrimps were challenged with V. harveyi bacteria (1 × 107cells mL-1), which were isolated and propagated from the infected shrimps. The shrimps fed on diets with methanolic extraction of 2.5 mL kg-1had significantly (P < 0.001) higher survival rate (76%), specific growth rate (4.26 ± 0.11%) and better food conversion ratio (1.5) than the other groups. This study indicates that addition of methanolic herbal extracts of 2.5 mL kg-1can positively influence the immune response of tiger shrimp against V. harveyi infection.
authors.affiliation: Institute of Marine Sciences and Fisheries, University of Chittagong, Chittagong 4331, Bangladesh.
authors.forename: Sheikh
authors.initials: S
authors.lastname: AftabUddin
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic; Department of Oceanography, Noakhali Science and Technology University, Sonapur, Noakhali 3814, Bangladesh. Electronic address: siddique@frov.jcu.cz.
authors.forename: Mohammad Abdul Momin
authors.initials: MAM
authors.lastname: Siddique
authors.affiliation: Institute of Marine Sciences and Fisheries, University of Chittagong, Chittagong 4331, Bangladesh.
authors.forename: Shaharin Salma
authors.initials: SS
authors.lastname: Romkey
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic; Biology Department, University of Oklahoma, Norman, OK 73019, United States.
authors.forename: William L
authors.initials: WL
authors.lastname: Shelton
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004032:Diet; D019587:Dietary Supplements / Q000032:analysis; D007113:Immunity, Innate / Q000187:drug effects; D008136:Longevity / Q000187:drug effects; D033561:Penaeidae / Q000187:drug effects* / Q000382:microbiology* / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D014733:Vibrio / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: Antibacterial function; Herbal plant extracts; Immunoprotection; Penaeus monodon