title: Fungal Biomass Protein Production fromTrichoderma harzianumUsing Rice Polishing.
abstract: Industrially important enzymes and microbial biomass proteins have been produced from fungi for more than 50 years. High levels of crude protein as much as 45% are present in fungal biomass with balanced essential amino acids. The aim of this study was to access the potential ofTrichoderma harzianumto produce fungal biomass protein from rice polishings. Maximum biomass yield was obtained at 5% (w/v) rice polishings after 72 h of incubation at 28°C at pH 4. Carbon and nitrogen ratio of 20 : 1 gave significantly higher production of fungal biomass protein. The FBP in the 75 L fermenter contained 49.50% crude protein, 32.00% true protein, 19.45% crude fiber, 9.62% ash, 11.5% cellulose content, and 0.325% RNA content. The profile of amino acids of final FBP exhibited that all essential amino acids were present in great quantities. The FBP produced by this fungus has been shown to be of good nutritional value for supplementation to poultry. The results presented in this study have practical implications in that the fungusT. harzianumcould be used successfully to produce fungal biomass protein using rice polishings.
authors.affiliation: Department of Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan; University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
authors.forename: Sibtain
authors.identifier: 0000-0003-0708-3278
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: Department of Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan; Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, Pakistan.
authors.forename: Ghulam
authors.initials: G
authors.lastname: Mustafa
authors.affiliation: University of Veterinary and Animal Sciences, Sub-Campus Jhang, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, Pakistan.
authors.forename: Muhammad Ibrahim
authors.identifier: 0000-0001-9231-5664
authors.initials: MI
authors.lastname: Rajoka
mesh_terms: D000821:Animal Feed; D000818:Animals; D018533:Biomass; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D012275:Oryza / Q000737:chemistry; D011200:Poultry; D014242:Trichoderma / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D002244:Carbon; D002482:Cellulose; D009584:Nitrogen