title: Production of jet fuel precursor monoterpenoids from engineered Escherichia coli.
abstract: Monoterpenes (C10isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C15products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8-cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high-value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703-1712. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Daniel
authors.initials: D
authors.lastname: Mendez-Perez
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Jorge
authors.initials: J
authors.lastname: Alonso-Gutierrez
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Margaux
authors.initials: M
authors.lastname: Molinas
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Edward E K
authors.initials: EEK
authors.lastname: Baidoo
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: George
authors.initials: G
authors.lastname: Wang
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Leanne J G
authors.initials: LJG
authors.lastname: Chan
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Adams
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Petzold
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.|The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Horsholm, Denmark.|Department of Chemical & Biomolecular Engineering, University of California, Berkeley, California.|Department of Bioengineering, University of California, Berkeley, California.
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
authors.affiliation: Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.|Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
authors.forename: Taek S
authors.identifier: 0000-0002-0764-2626
authors.initials: TS
authors.lastname: Lee
mesh_terms: D011756:Diphosphates / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D024861:Genetic Enhancement / Q000379:methods*; D051228:Geranyltranstransferase / Q000235:genetics*; D006838:Hydrocarbons / Q000138:chemical synthesis*; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism*
chemical_list: D011756:Diphosphates; D004224:Diterpenes; D029968:Escherichia coli Proteins; D006838:Hydrocarbons; D039821:Monoterpenes; D011106:Polyisoprenyl Phosphates; D011994:Recombinant Proteins; D012717:Sesquiterpenes; C511282:geranyl diphosphate; C004808:farnesyl pyrophosphate; C029662:JP5 jet fuel; D051228:Geranyltranstransferase; C573354:IspA protein, E coli
keywords: 1,8-cineole; jet fuel; linalool; metabolic engineering; mevalonate pathway; monoterpenes