title: Clonal analysis as a prognostic factor in multiple oral squamous cell carcinoma.
abstract: A novel classification based on molecular methods to assess clonality defines three types of secondary oral squamous cell carcinoma (OSCC): second primary tumour (SPT) independent from the index tumour, local recurrence (LR), clonally related to the primary tumour, and second field tumour (SFT), derived from the same genetically altered mucosal field as the primary tumour. The present study applied mtDNA analysis in a group of patients experiencing a second loco-regional neoplastic manifestation. The purpose was to differentiate secondary tumours into LRs, SPTs and SFTs and evaluate the prognostic impact in terms of survival rate.
  
  
  
  The study population comprised 23 patients who experienced a second neoplastic lesion after a surgical resection of primary OSCC. mtDNA D-loop analysis was applied in paired neoplastic lesions and in clinically and histologically normal mucosa. On the basis of mtDNA results, the second OSCC was classified as LR or SPT or SFT. Disease-free survival was defined as the duration between the appearance of the second neoplastic lesion and death of disease, or last follow-up visit.
  
  
  
  Seven secondary tumours were classified as LR, 12 as SFT, 4 as SPT. An altered mucosal field proved a variable significantly related to a better survival rate (p<0.05); 2/12 (16.6%) SFT events failed as compared to 5/7 LRs (71.4%) and 3/4 SPTs (75%).
  
  
  
  mtDNA analysis may be considered a useful tool to differentiate secondary tumours and might influence the choice of the most appropriate treatment in patients with multiple OSCCs.
authors.affiliation: Department of Biomedical and Neuromotor Sciences, Section of Oral Sciences, University of Bologna, Italy. Electronic address: davide.gissi@unibo.it.
authors.forename: D B
authors.initials: DB
authors.lastname: Gissi
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, Section of Maxillofacial Surgery at Policlinico S. Orsola-Malpighi, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Tarsitano
authors.affiliation: Department of Biomedical and Neuromotor Sciences, Section of Anatomic Pathology at Bellaria Hospital, University of Bologna, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Leonardi
authors.affiliation: Department of Biomedical and Neuromotor Sciences, Section of Oral Sciences, University of Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Gabusi
authors.affiliation: Unit of Maxillofacial Surgery, Bellaria Hospital, Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Neri
authors.affiliation: Department of Biomedical and Neuromotor Sciences, University of Bologna, Section of Maxillofacial Surgery at Policlinico S. Orsola-Malpighi, Bologna, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Marchetti
authors.affiliation: Department of Biomedical and Neuromotor Sciences, Section of Oral Sciences, University of Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Montebugnoli
authors.affiliation: Department of Biomedical and Neuromotor Sciences, Section of Anatomic Pathology at Bellaria Hospital, University of Bologna, Italy.
authors.forename: M P
authors.initials: MP
authors.lastname: Foschini
authors.affiliation: Department of Biomedical and Neuromotor Sciences, Section of Anatomic Pathology at Bellaria Hospital, University of Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Morandi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000628:therapy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology* / Q000628:therapy; D011379:Prognosis; D015996:Survival Rate; D055815:Young Adult
keywords: Local recurrence; Oral squamous cell carcinoma; Prognosis; Second field tumour; Second primary tumour; mtDNA