title: MK-801-Treated Oligodendrocytes as a Cellular Model to Study Schizophrenia.
abstract: Glutamate is the most important excitatory neurotransmitter in the brain. The N-methyl-D-aspartate (NMDA) subtype of glutamate receptor is found both in neurons and glial cells such as oligodendrocytes, which have been shown to be dysfunctional in schizophrenia. For this reasons, the oligodendrocyte MO3.13 cell line has been used to study glutamatergic dysfunction as a model of schizophrenia using the NMDA receptor antagonists such as MK-801 to block receptor function. Here, we describe a comprehensive protocol for culturing and carrying out proteomic analyses of MK-801-treated MO3.13 cells as a means of identifying potential new biomarkers and targets for drug discovery in schizophrenia research.
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Brandão-Teles
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.|UNICAMP's Neurobiology Center, Campinas, SP, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil. jscassoli@gmail.com.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Cassoli
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002853:Chromatography, Liquid / Q000379:methods; D016291:Dizocilpine Maleate / Q000633:toxicity*; D001330:Electronic Data Processing; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity*; D018698:Glutamic Acid / Q000502:physiology; D006801:Humans; D006822:Hybrid Cells; D036103:Nanotechnology / Q000379:methods; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism; D012559:Schizophrenia / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D009435:Synaptic Transmission / Q000187:drug effects; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D018691:Excitatory Amino Acid Antagonists; D018698:Glutamic Acid; D016291:Dizocilpine Maleate
keywords: MK-801; MO3.13 cells; Mass spectrometry; NMDA receptor; Oligodendrocytes; Proteomics; Schizophrenia