title: Host Factors in the Infection Cycle ofBamboo mosaic virus.
abstract: To complete the infection cycle efficiently, the virus must hijack the host systems in order to benefit for all the steps and has to face all the defense mechanisms from the host. This review involves a discussion of how these positive and negative factors regulate the viral RNA accumulation identified for theBamboo mosaic virus(BaMV), a single-stranded RNA virus. The genome of BaMV is approximately 6.4 kb in length, encoding five functional polypeptides. To reveal the host factors involved in the infection cycle of BaMV, a few different approaches were taken to screen the candidates. One of the approaches is isolating the viral replicase-associated proteins by co-immunoprecipitation with the transiently expressed tagged viral replicase in plants. Another approach is using the cDNA-amplified fragment length polymorphism technique to screen the differentially expressed genes derived fromN. benthamianaplants after infection. The candidates are examined by knocking down the expression in plants using theTobacco rattle virus-based virus-induced gene silencing technique following BaMV inoculation. The positive or negative regulators could be described as reducing or enhancing the accumulation of BaMV in plants when the expression levels of these proteins are knocked down. The possible roles of these host factors acting on the accumulation of BaMV will be discussed.
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University Taichung, Taiwan.
authors.forename: Ying-Ping
authors.initials: YP
authors.lastname: Huang
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University Taichung, Taiwan.
authors.forename: I-Hsuan
authors.initials: IH
authors.lastname: Chen
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University Taichung, Taiwan.
authors.forename: Ching-Hsiu
authors.initials: CH
authors.lastname: Tsai
keywords: Bamboo mosaic virus; defense proteins; host factors; virus movement; virus replication