title: Human exposure to Bisphenol A and liver health status: Quantification of urinary and circulating levels by LC-MS/MS.
abstract: A selective and highly sensitive analytical methodology for determination of Bisphenol A in human plasma was developed and validated. The method was based on selective liquid/solid extraction, combined with liquid chromatography-electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode and negative ionization. The linearity of the detector response was verified in human plasma over the concentration range 0.100-200ngmL-1. The detection limit was 0.03ngmL-1and the quantification limit was 0.100ngmL-1. The analytical features of the proposed in-house validated method were satisfactory: precision was <10% and recoveries were around 84-104%. The matrix effect was studied and compensated using deuterated labeled standard. The applicability of the proposed method was demonstrated analyzing human plasma samples from individuals affected by non-alcoholic fatty liver disease. Bisphenol A was detected above the detection limit in all samples. The data show a persistence of unconjugated Bisphenol A levels in plasma and indicate a chronic Bisphenol A exposure of the target organ, suggesting an association between liver health status and Bisphenol A exposure. The results from our study are valuable for further investigation with large sample size and longitudinal study designs, necessary to confirm the observed association.
authors.affiliation: Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Nicolucci
authors.affiliation: Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Errico
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Hepatogastroenterology, University of Campania "L. Vanvitelli", Via Pansini, 5, 80131 Naples, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Federico
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Hepatogastroenterology, University of Campania "L. Vanvitelli", Via Pansini, 5, 80131 Naples, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Dallio
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Hepatogastroenterology, University of Campania "L. Vanvitelli", Via Pansini, 5, 80131 Naples, Italy.
authors.forename: Carmelina
authors.initials: C
authors.lastname: Loguercio
authors.affiliation: Department of Experimental Medicine, University of Campania "L. Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy. Electronic address: nadia.diano@unicampania.it.
authors.forename: Nadia
authors.initials: N
authors.lastname: Diano
mesh_terms: D001559:Benzhydryl Compounds; D006304:Health Status; D006801:Humans; D008099:Liver; D008137:Longitudinal Studies; D010636:Phenols; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Human plasma; Liquid chromatography–tandem mass spectrometry; Liver