title: LC-MS phenolic profiling combined with multivariate analysis as an approach for the characterization of extra virgin olive oils of four rare Tunisian cultivars during ripening.
abstract: In this work, the phenolic composition of four rare cultivars grown under the same agronomical and environmental conditions was studied. This is to test the effects of cultivars and ripening index essentially on phenolic composition in olive oils as well as tocopherols composition, organoleptic profiling and oxidative properties. Furthermore, some agronomical traits were determined in which a general increase in the size of the fruit and oil contents were recorded for all cultivars. The phenolic fractions were identified and quantified using liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MS/MS) in multiple reaction monitoring mode (MRM). A total of 13 phenolic compounds belonging to different chemical families were determined. Qualitative and quantitative differences in phenolic composition were observed among cultivars and also among sampling times. On the contrary to the agronomical traits, a general decrease (p<0.05) of total phenolic compounds was observed during maturation. Likewise, a decrease in tocopherols concentrations and oxidative properties was observed.
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieur de Sfax, Université de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Samia
authors.initials: S
authors.lastname: Ben Brahim
authors.affiliation: Department of Food Engineering, Faculty of Engineering and Natural Sciences, Adana University of Science and Technology, 01100 Adana, Turkey.
authors.forename: Hasim
authors.initials: H
authors.lastname: Kelebek
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieur de Sfax, Université de Sfax, BP 1173, 3038 Sfax, Tunisia.
authors.forename: Sonda
authors.initials: S
authors.lastname: Ammar
authors.affiliation: Institut de l'olivier à Zarzis, Tunisia.
authors.forename: Mounir
authors.initials: M
authors.lastname: Abichou
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieur de Sfax, Université de Sfax, BP 1173, 3038 Sfax, Tunisia; Institut Supérieur de Biotechnologie de Sfax, Université de Sfax, BP 1175, 3038 Sfax, Tunisia. Electronic address: mohamed.bouaziz@fsg.rnu.tn.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bouaziz
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D015999:Multivariate Analysis; D000069463:Olive Oil / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014416:Tunisia
chemical_list: D000069463:Olive Oil; D010636:Phenols
keywords: 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (PubChem CID: 2735032); Acetic acid (PubChem CID: 176); Acetonitrile (PubChem CID: 6342); Alpha-tocopherol (PubChem CID: 14985); Apigenin (PubChem CID: 5280443); Beta-tocopherol (PubChem CID: 6857447); Caffeic acid (PubChem CID: 689043); Cyclohexane (PubChem CID: 8078); Delta-tocopherol (PubChem CID: 92094); Diosmetin (PubChem CID: 5281612); Ferulic acid (PubChem CID: 445858); Four rare Tunisian cultivar, ripening index; Gamma-tocopherol (PubChem CID: 92729); Hexane (PubChem CID: 8058); LC-DAD-ESI-MS/MS; Luteolin (PubChem CID: 5280445); Methanol (PubChem CID: 887); Oxidative state; Phenolic compounds; Protocatechuic acid (PubChem CID: 72); Tyrosol (PubChem CID: 10393); p-Coumaric acid (PubChem CID: 637542)