title: A Two-Stage Dissociation System for Multilayer Imaging of Cancer Biomarker-Synergic Networks in Single Cells.
abstract: The monitoring of cancer biomarkers is crucial to the early detection of cancer. However, a limiting factor in biomarker analysis is the ability to obtain the multilayered information of various biomarker molecules located at different parts of cells from the plasma membrane to the cytoplasm. A two-stage dissociation nanoparticle system based on multifunctionalized polydopamine-coated gold nanoparticles (Au@PDA NPs) is reported, which allows for the two-stage imaging of cancer biomarkers in single cells. We demonstrate the feasibility of this strategy on sialic acids (SAs), p53 protein, and microRNA-21 (miRNA-21) in MCF-7 breast cancer cells by two custom-built probes. Furthermore, the multicolor fluorescence information extracted is used for the monitoring of biomarker expression changes under different drug combinations, which allows us to investigate the complex interactions between various cancer biomarkers and to describe the cancer biomarker-synergic networks in single cells.
authors.affiliation: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.
authors.forename: Ruo-Can
authors.identifier: 0000-0002-1039-9866
authors.initials: RC
authors.lastname: Qian
authors.affiliation: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D007211:Indoles / Q000737:chemistry; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D012794:Sialic Acids / Q000032:analysis; D059010:Single-Cell Analysis / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D007211:Indoles; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D011108:Polymers; D012794:Sialic Acids; D016159:Tumor Suppressor Protein p53; C568283:polydopamine; D006046:Gold
keywords: cancer biomarkers; cancer pathway; single-cell imaging; two-stage disassembly