title: A rapid ATR-FTIR spectroscopic method for detection of sibutramine adulteration in tea and coffee based on hierarchical cluster and principal component analyses.
abstract: Sibutramine may be illicitly included in herbal slimming foods and supplements marketed as "100% natural" to enhance weight loss. Considering public health and legal regulations, there is an urgent need for effective, rapid and reliable techniques to detect sibutramine in dietetic herbal foods, teas and dietary supplements. This research comprehensively explored, for the first time, detection of sibutramine in green tea, green coffee and mixed herbal tea using ATR-FTIR spectroscopic technique combined with chemometrics. Hierarchical cluster analysis and PCA principle component analysis techniques were employed in spectral range (2746-2656cm-1) for classification and discrimination through Euclidian distance and Ward's algorithm. Unadulterated and adulterated samples were classified and discriminated with respect to their sibutramine contents with perfect accuracy without any false prediction. The results suggest that existence of the active substance could be successfully determined at the levels in the range of 0.375-12mg in totally 1.75g of green tea, green coffee and mixed herbal tea by using FTIR-ATR technique combined with chemometrics.
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey.
authors.forename: Nur
authors.initials: N
authors.lastname: Cebi
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey. Electronic address: mtyilmaz@yildiz.edu.tr.
authors.forename: Mustafa Tahsin
authors.initials: MT
authors.lastname: Yilmaz
authors.affiliation: Yıldız Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, 34210 İstanbul, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Sagdic
mesh_terms: D016000:Cluster Analysis; D003069:Coffee / Q000737:chemistry*; D003503:Cyclobutanes / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013662:Tea / Q000737:chemistry*
chemical_list: D003069:Coffee; D003503:Cyclobutanes; D013662:Tea; C058254:sibutramine
keywords: ATR-FTIR; Adulteration; Chemometrics; Sibutramine; Slimming tea