title: RP-HPLC enantioseparation of β-adrenolytics using micellar mobile phase without organic solvents.
abstract: Enantioseparation of a few commonly administered racemic β-adrenolytics (namely, carvedilol, betaxolol, salbutamol and bisoprolol) has been achieved using a water micellar mobile phase containing surfactants (sodium dodecyl sulphate and Brij-35) without organic solvents as a new approach in RP-HPLC. Two chiral derivatizing reagents based on enantiomerically pure (S)-(-)-levofloxacin were synthesized using N-hydroxysuccinimide and N-hydroxybenzotriazole as the activation auxiliaries. Diastereomeric derivatives of the chosen β-adrenolytics were synthesized under microwave irradiation in a very short reaction time. The (S)-(-)-levofloxacin moiety enhanced molar absorbance of the diastereomeric derivatives resulting in very low limit of detection (1.618 and 4.902 ng/mL, respectively, for diastereomeric derivatives of (RS)-betaxolol and better resolution with lower retention times (for all the analytes), in comparison to literature reports. There was 15-20 times less consumption of mobile phase because of lower retention time.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India.
authors.forename: Shiv
authors.initials: S
authors.lastname: Alwera
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India.
authors.forename: Ravi
authors.identifier: http://orcid.org/0000-0001-5098-9759
authors.initials: R
authors.lastname: Bhushan
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008823:Micelles; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D000319:Adrenergic beta-Antagonists; D008823:Micelles; D013501:Surface-Active Agents
keywords: (S)-(−)-levofloxacin; Diastereomeric derivatives; RP-HPLC; Surfactants; water micellar mobile phase; β-adrenolytics