title: Iron Oxide Nanoparticles Coated with a Phosphorothioate Oligonucleotide and a Cationic Peptide: Exploring Four Different Ways of Surface Functionalization.
abstract: The superparamagnetic iron oxide nanoparticles (SPIONs) have great potential in therapeutic and diagnostic applications. Due to their superparamagnetic behavior, they are used clinically as a Magnetic Resonance Imaging (MRI) contrast agent. Iron oxide nanoparticles are also recognized todays as smart drug-delivery systems. However, to increase their specificity, it is essential to functionalize them with a molecule that effectively targets a specific area of the body. Among the molecules that can fulfill this role, peptides are excellent candidates. Oligonucleotides are recognized as potential drugs for various diseases but suffer from poor uptake and intracellular degradation. In this work, we explore four different strategies, based on the electrostatic interactions between the different partners, to functionalize the surface of SPIONs with a phosphorothioate oligonucleotide (ODN) and a cationic peptide labeled with a fluorophore. The internalization of the nanoparticles has been evaluatedin vitroon RAW 264.7 cells. Among these strategies, the "«one-step assembly»",i.e., the direct complexation of oligonucleotides and peptides on iron oxide nanoparticles, provides the best way of coating for the internalization of the nanocomplexes.
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, UFR de Santé, Médecine et Biologie Humaine, F-93017 Bobigny, France. frederic.geinguenaud@univ-paris13.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Geinguenaud
authors.affiliation: Université Paris Descartes, Laboratoire de Recherches Biochirurgicales, Hôpital Européen Georges Pompidou, F-75015 Paris, France. claire.banissi@aphp.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Banissi
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, UFR de Santé, Médecine et Biologie Humaine, F-93017 Bobigny, France. claire.banissi@aphp.fr.|Université Paris Descartes, Laboratoire de Recherches Biochirurgicales, Hôpital Européen Georges Pompidou, F-75015 Paris, France. claire.banissi@aphp.fr.
authors.forename: Antoine F
authors.initials: AF
authors.lastname: Carpentier
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, UFR de Santé, Médecine et Biologie Humaine, F-93017 Bobigny, France. laurence.motte@univ-paris13.fr.
authors.forename: Laurence
authors.initials: L
authors.lastname: Motte
keywords: iron oxide nanoparticles; oligonucleotide; peptide; surface functionalization