title: Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover.
abstract: High enzyme loading and low productivity are two major issues impeding low cost ethanol production from lignocellulosic biomass. This work applied rapid bioconversion with integrated recycle technology (RaBIT) and extractive ammonia (EA) pretreatment for conversion of corn stover (CS) to ethanol at high solids loading. Enzymes were recycled via recycling unhydrolyzed solids. Enzymatic hydrolysis with recycled enzymes and fermentation with recycled yeast cells were studied. Both enzymatic hydrolysis time and fermentation time were shortened to 24 h. Ethanol productivity was enhanced by two times and enzyme loading was reduced by 30%. Glucan and xylan conversions reached as high as 98% with an enzyme loading of as low as 8.4 mg protein per g glucan. The overall ethanol yield was 227 g ethanol/kg EA-CS (191 g ethanol/kg untreated CS). Biotechnol. Bioeng. 2017;114: 1713-1720. © 2017 Wiley Periodicals, Inc.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094, China.|Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, Michigan.
authors.forename: Mingjie
authors.identifier: 0000-0002-9493-305X
authors.initials: M
authors.lastname: Jin
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, Michigan.|Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, Michigan.
authors.forename: Leonardo
authors.initials: L
authors.lastname: da Costa Sousa
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, Michigan.
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Dale
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, Michigan.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D019149:Bioreactors / Q000382:microbiology; D002480:Cellulase / Q000737:chemistry*; D000431:Ethanol / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D059625:Liquid-Liquid Extraction / Q000379:methods; D035261:Plant Components, Aerial / Q000737:chemistry / Q000382:microbiology*; D059027:Recycling / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D018511:Systems Integration; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D000641:Ammonia; D008031:Lignin; D002480:Cellulase
keywords: EA pretreatment; RaBIT process; enzymatic hydrolysis; enzyme recycling; ethanol fermentation; lignocellulosic ethanol