title: Honokiol-loaded polymeric nanoparticles: an active targeting drug delivery system for the treatment of nasopharyngeal carcinoma.
abstract: The purpose of this study was to develop a novel drug delivery system for a sustained and targeted delivery of honokiol (HK) to the nasopharyngeal carcinoma (NPC) HNE-1 cell lines, since the folate receptor (FR) is over-expressed on their surface. Emulsion solvent evaporation was used to develop the active targeting nanoparticles-loaded HK (ATNH) using copolymerpoly (ɛ-caprolactone)-poly (ethyleneglycol)-poly (ɛ-caprolactone) (PCEC), which was modified with folate (FA) by introducing Polythylenimine (PEI). ATNH characterization, including particle size distribution, morphology, drug loading, encapsulation efficiency and drug release, was performed. Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) were employed to evaluate the shape and construction, respectively. MTT assay, cell uptake study and apoptosis test were assayed to detect the antitumor properties and targeting uptake by HNE-1 cells in vitro. Cell-cycle redistribution,18F-FDG PET/CT and immunohistochemistry were performed in vivo. The ATNH we developed were successfully synthesized and showed a suitable size distribution, high encapsulation efficiency, gradual release, and targeting uptake by the cells in vitro. Moreover, ATNH significantly inhibited tumor growth, metabolism, proliferation, micro-vessel generation, and caused cell-cycle arrest at G1phase. Thus, these nanoparticles we developed might represent a novel formulation for HK delivery and a promising potential therapy in the treatment of cancer.
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: XiaoLing
authors.initials: X
authors.lastname: Ni
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: LongXia
authors.initials: L
authors.lastname: Chen
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: PeiRong
authors.initials: P
authors.lastname: Ren
authors.affiliation: b Department of Nuclear Medicine , the Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: b Department of Nuclear Medicine , the Affiliated Hospital of Southwest Medical University , Luzhou , China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: ShaoZhi
authors.initials: S
authors.lastname: Fu
authors.affiliation: a Department of Oncology , the Affiliated Hospital of Southwest Medical University , Luzhou , China and.
authors.forename: JingBo
authors.initials: J
authors.lastname: Wu
mesh_terms: D001713:Biphenyl Compounds; D002277:Carcinoma; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D017705:Lignans; D053758:Nanoparticles; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms; D010316:Particle Size; D011092:Polyethylene Glycols; D000072078:Positron Emission Tomography Computed Tomography; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D017705:Lignans; D011092:Polyethylene Glycols; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols
keywords: Honokiol; NPC; active targeting; folate; nanoparticles