title: Immune status of children with obstructive sleep apnea/hypopnea syndrome.
abstract: OBJECTIVE
  We aimed to evaluate the immune status of children with obstructive sleep apnea/hypopnea syndrome (OSAHS).
  
  
  METHODS
  Fifty children with OSAHS having the symptoms of "snoring, mouth breathing and suffocating during sleep", who were admitted in our hospital from May 2014 to May 2016, were randomly selected. Another 52 healthy, age- and gender-matched children were enrolled as control subjects after taking informed consent. After admission, the peripheral venous blood was collected. T cell subsets and cytokines were analyzed by flow cytometry. Immunoglobulin and complement levels were detected by immunoassay analyzer.
  
  
  RESULTS
  The percentage of CD8+T lymphocytes in children with OSAHS was (26.47 ± 1.52)% which was significantly higher than that of control group ((21.94 ± 1.92)%) (P<0.05). OSAHS group had a significantly lower CD4+/CD8+ratio (1.24 ± 0.12) than that of control group (1.45 ± 0.11) (P<0.05). The two groups had similar percentages of CD3+and CD4+T lymphocytes (P>0.05). OSAHS group had significantly higher serum levels of IL-4, IL-6, IL-10 and IFN-γ than those of control group (P<0.05), but their IL-2 and TNF-α levels were similar (P>0.05). The serum IgA and C3 levels of OSAHS group significantly exceeded those of control group (P<0.05), but their IgG, IgM and C4 levels were similar (P>0.05).
  
  
  CONCLUSIONS
  Children with OSAHS had increased percentage of CD8+T lymphocytes and decreased CD4+/CD8+ratio, suggesting this group had poor immune function. Increase in humoral immune-related indices IL-4, IL-6, IL-10 and IFN-γ indicated the occurrence of oxidative stress and systemic inflammatory status.
authors.affiliation: Zihe Zhang, Department of Otolaryngology, Qilu Children's Hospital of Shandong University, Ji'nan 250022, Shandong Province, China.
authors.forename: Zihe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Chunguang Wang, Department of Laboratory Medicine, Anqiu People's Hospital, Weifang 262100, Shandong Province, China.
authors.forename: Chunguang
authors.initials: C
authors.lastname: Wang
keywords: Children; Hypopnea syndrome; Immune status; Obstructive sleep apnea