title: Effects of different preservation methods on inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers in botanic samples.
abstract: To evaluate the effects of different preservation methods (stored in a -20°C ice chest, preserved in liquid nitrogen and dried in silica gel) on inter simple sequence repeat (ISSR) or random amplified polymorphic DNA (RAPD) analyses in various botanical specimens (including broad-leaved plants, needle-leaved plants and succulent plants) for different times (three weeks and three years), we used a statistical analysis based on the number of bands, genetic index and cluster analysis. The results demonstrate that methods used to preserve samples can provide sufficient amounts of genomic DNA for ISSR and RAPD analyses; however, the effect of different preservation methods on these analyses vary significantly, and the preservation time has little effect on these analyses. Our results provide a reference for researchers to select the most suitable preservation method depending on their study subject for the analysis of molecular markers based on genomic DNA.
authors.affiliation: National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, Jilin University, Changchun, Jilin Province 130012, People's Republic of China; School of Life Sciences, Jilin University, Changchun, Jilin Province 130012, People's Republic of China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
authors.affiliation: National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, Jilin University, Changchun, Jilin Province 130012, People's Republic of China; School of Life Sciences, Jilin University, Changchun, Jilin Province 130012, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, Jilin University, Changchun, Jilin Province 130012, People's Republic of China; School of Life Sciences, Jilin University, Changchun, Jilin Province 130012, People's Republic of China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, Jilin University, Changchun, Jilin Province 130012, People's Republic of China; Liaoning Technical University College of Science, Fuxin, Liaoning Province 123000, People's Republic of China.
authors.forename: Fangliang
authors.initials: F
authors.lastname: Li
authors.affiliation: Changbai Mountain Academy of Sciences, Erdao, Antu, Jilin Province 133613, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, Jilin University, Changchun, Jilin Province 130012, People's Republic of China; School of Life Sciences, Jilin University, Changchun, Jilin Province 130012, People's Republic of China. Electronic address: chenxiajlu@163.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
mesh_terms: D018895:Microsatellite Repeats; D010944:Plants / Q000737:chemistry / Q000235:genetics*; D011309:Preservation, Biological / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
keywords: Botanic samples; ISSR; Preservation methods; RAPD