title: Efficient killing of radioresistant breast cancer cells by cytokine-induced killer cells.
abstract: Recurrence of breast cancer after radiotherapy may be partly explained by the presence of radioresistant cells. Thus, it would be desirable to develop an effective therapy against radioresistant cells. In this study, we demonstrated the intense antitumor activity of cytokine-induced killer cells against MCF-7 and radioresistant MCF-7 cells, as revealed by cytokine-induced killer-mediated cytotoxicity, tumor cell proliferation, and tumor invasion. Radioresistant MCF-7 cells were more susceptible to cytokine-induced killer cell killing. The stronger cytotoxicity of cytokine-induced killer cells against radioresistant MCF-7 cells was dependent on the expression of major histocompatibility complex class I polypeptide-related sequence A/B on radioresistant MCF-7 cells after exposure of cytokine-induced killer cells to sensitized targets. In addition, we demonstrated that cytokine-induced killer cell treatment sensitized breast cancer cells to chemotherapy via the downregulation of TK1, TYMS, and MDR1. These results indicate that cytokine-induced killer cell treatment in combination with radiotherapy and/or chemotherapy may induce synergistic antitumor activities and represent a novel strategy for breast cancer.
authors.affiliation: 1 Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Guo
authors.affiliation: 1 Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Danni
authors.initials: D
authors.lastname: Zhu
authors.affiliation: 2 The Affiliated Cardiovascular Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Bu
authors.affiliation: 1 Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wei
authors.affiliation: 1 Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Changyou
authors.initials: C
authors.lastname: Li
authors.affiliation: 1 Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Daiqing
authors.initials: D
authors.lastname: Gao
authors.affiliation: 3 Department of Gynaecology, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Wei
authors.affiliation: 4 Department of Oncology, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Xuezhen
authors.initials: X
authors.lastname: Ma
authors.affiliation: 1 Biotherapy Center, Qingdao Central Hospital, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000096:biosynthesis; D001943:Breast Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D064987:Cell- and Tissue-Based Therapy; D055612:Cytokine-Induced Killer Cells / Q000276:immunology / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009364:Neoplasm Recurrence, Local / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D011836:Radiation Tolerance; D013937:Thymidine Kinase / Q000096:biosynthesis; D013940:Thymidylate Synthase / Q000096:biosynthesis
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; C000600377:TYMS protein, human; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; C092717:thymidine kinase 1
keywords: Cytokine-induced killer cells; breast cancer; immunotherapy; radioresistance