title: Trapping multiple dual mode centrifugal partition chromatography for the separation of intermediately-eluting components: Operating parameter selection.
abstract: The preparative separation of intermediately-eluting components in liquid-liquid chromatography is commonly performed with isocratic batch injections, a technique which often leads to low yield and/or purity as a result of peak overlap. Two-column trapping multiple dual mode centrifugal partition chromatography, an alternative discontinuous method for the separation of a mixture into three product fractions (early-, intermediately-, and late-eluting components) at full recovery, is presented in this work. A mathematical shortcut method based on equilibrium theory assumptions is derived for the determination of the key operating parameters (i.e., step durations and number of steps). The feasibility of the technique and the accompanying short-cut method is demonstrated by proof-of-concept experiments for the separation of two paraben model mixtures.
authors.affiliation: Biothermodynamics, TUM School of Life Sciences Weihenstephan, Technische Universität München, Gregor-Mendel-Str. 4, 85354 Freising, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Goll
authors.affiliation: Biothermodynamics, TUM School of Life Sciences Weihenstephan, Technische Universität München, Gregor-Mendel-Str. 4, 85354 Freising, Germany.
authors.forename: Raena
authors.initials: R
authors.lastname: Morley
authors.affiliation: Biothermodynamics, TUM School of Life Sciences Weihenstephan, Technische Universität München, Gregor-Mendel-Str. 4, 85354 Freising, Germany. Electronic address: mirjana.minceva@tum.de.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Minceva
mesh_terms: D002498:Centrifugation; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D003377:Countercurrent Distribution; D008956:Models, Chemical; D010226:Parabens / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D010226:Parabens
keywords: Centrifugal partition chromatography; Countercurrent chromatography; Liquid-liquid chromatography; Ternary paraben separation; Trapping multiple dual mode