title: Antioxidative cellular response of lepidopteran ovarian cells to photoactivated alpha-terthienyl.
abstract: Photodynamic sensitizers as useful alternative agents have been used for population control against insect pests, and the response of insect ovarian cells towards the photosensitizers is gaining attention because of the next reproduction. In this paper, antioxidative responses of lepidopteran ovarian Tn5B1-4 and Sf-21 cells to photoactivated alpha-terthienyl (PAT) are investigated. PAT shows positive inhibitory cytotoxicity on the two ovarian cells, and its inhibition on cell viability is enhanced as the concentrations are increased and the irradiation time is extended. Median inhibitory concentrations (IC50) are 3.36μg/ml to Tn5B1-4 cells, and 3.15μg/ml to Sf-21 cells at 15min-UV-A irradiation 2h-dark incubation. Under 10.0μg/ml PAT exposure, 15min-UV-A irradiation excites higher ROS production than 5min-UV-A irradiation does in the ovarian cells, the maximum ROS content is about 7.1 times in Tn5B1-4 cells and 4.3 times in Sf-21 cells, and the maximum malondialdehyde levels in Tn5B1-4 and Sf-21 cells are about 1.47- and 1.36-fold higher than the control groups, respectively. Oxidative stress generated by PAT strongly decreases the activities of POD, SOD and CAT, and induces an accumulation of Tn5B1-4 cells in S phase and Sf-21 cells in G2/M phase in a concentration-dependent fashion. Apoptosis accumulation of Tn5B1-4 cells and the persistent post-irradiation cytotoxicity are further observed, indicating different antioxidative tolerance and arrest pattern of the two ovarian cells towards the cytotoxicity of PAT.
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: qchuang@ecust.edu.cn.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Yun
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Ciying
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D005260:Female; D005434:Flow Cytometry; D007306:Insecticides / Q000494:pharmacology* / Q000528:radiation effects; D007915:Lepidoptera / Q000166:cytology / Q000187:drug effects*; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013876:Thiophenes / Q000494:pharmacology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D007306:Insecticides; D013876:Thiophenes; C019101:alpha-terthienyl
keywords: Alpha-terthienyl; Antioxidative response; Cytotoxicity; Sf-21 cells; Tn5B1-4 cells