title: Cell wall dynamics modulate acetic acid-induced apoptotic cell death ofSaccharomyces cerevisiae.
abstract: Acetic acid triggers apoptotic cell death inSaccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wall integrity pathways were the major mediators, especially the latter, which we characterized further. Screening downstream effectors of this pathway, namely targets of the transcription factor Rlm1p, highlighted decreased cell wall remodeling as particularly important for acetic acid resistance. Modulation of cell surface dynamics therefore emerges as a powerful strategy to increase acetic acid resistance, with potential application in industrial fermentations using yeast, and in biomedicine to exploit the higher sensitivity of colorectal carcinoma cells to apoptosis induced by acetate produced by intestinal propionibacteria.
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: António
authors.initials: A
authors.lastname: Rego
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Duarte
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: Flávio
authors.initials: F
authors.lastname: Azevedo
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Sousa
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Côrte-Real
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: Susana R
authors.initials: SR
authors.lastname: Chaves
keywords: CWI; MAPK; acetic acid; apoptosis; yeast