title: Patterns of stress response and tolerance based on transcriptome profiling of rice crown tissue under zinc deficiency.
abstract: Zinc (Zn) deficiency is the most prevalent micronutrient disorder in rice and leads to delayed development and decreased yield. Several studies have investigated how rice plants respond to Zn deficiency and examined the differences between Zn-efficient (ZE) and Zn-inefficient (ZI) genotypes. ZE genotypes reallocate more Zn to roots and are better at maintaining crown root development than ZI genotypes in response to Zn deficiency. However, little is known about the molecular mechanisms controlling these differences. Moreover, the role of the crown, the part of the stem from which crown roots emerge, has yet to be examined. In this study we highlight the molecular mechanisms triggered by early Zn deficiency in crown tissue through RNA sequencing of two contrasting groups of several ZE and ZI genotypes. This method allowed us to (i) identify several novel and well-known Zn transporters involved in Zn retranslocation from the crown to the shoot and roots in response to Zn deficiency; (ii) determine that Zn deficiency triggers the conversion of soluble sugars into starch; and (iii) detect several candidate genes possibly conferring Zn efficiency, including a monosaccharide transporter, a Zn finger domain-containing protein, a gibberellin-stimulated family protein and a plasma membrane polypeptide family protein.
authors.affiliation: Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Japan.
authors.forename: Amrit K
authors.initials: AK
authors.lastname: Nanda
authors.affiliation: Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Japan.
authors.forename: Vincent
authors.initials: V
authors.lastname: Pujol
authors.affiliation: Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Japan.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wissuwa
mesh_terms: D001692:Biological Transport; D020869:Gene Expression Profiling; D005838:Genotype; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological; D014158:Transcription, Genetic; D015032:Zinc / Q000172:deficiency*
chemical_list: D015032:Zinc
keywords: Crown; RNA-seq; deficiency; rice; starch; transporters; zinc.