title: Relevance of secretor status genotype and microbiota composition in susceptibility to rotavirus and norovirus infections in humans.
abstract: Host genetic factors, such as histo-blood group antigens (HBGAs), are associated with susceptibility to norovirus (NoV) and rotavirus (RV) infections. Recent advances point to the gut microbiome as a key player necessary for a viral pathogen to cause infection. In vitro NoV attachment to host cells and resulting infections have been linked to interactions with certain bacterial types in the gut microbiota. We investigated the relationship between host genotype, gut microbiota, and viral infections. Saliva and fecal samples from 35 adult volunteers were analysed for secretor status genotype, the gut microbiota composition by 16S rRNA gene sequencing, and salivary IgA titers to NoV and RV. Higher levels of IgA against NoV and RV were related to secretor-positive status. No significant differences were found between the FUT2 genotype groups, although the multivariate analysis showed a significant impact of host genotype on specific viral susceptibilities in the microbiome composition. A specific link was found between the abundance of certain bacterial groups, such as Faecalibacterium and Ruminococcus spp., and lower IgA titers against NoV and RV. As a conclusion, we can state that there is a link between host genetics, gut microbiota, and susceptibility to viral infections in humans.
authors.affiliation: Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Rodríguez-Díaz
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: García-Mantrana
authors.affiliation: Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Vila-Vicent
authors.affiliation: Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Gozalbo-Rovira
authors.affiliation: Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Buesa
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Monedero
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Collado
mesh_terms: D000328:Adult; D017250:Caliciviridae Infections / Q000235:genetics* / Q000382:microbiology*; D005243:Feces; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics*; D000069196:Gastrointestinal Microbiome; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D012400:Rotavirus Infections / Q000235:genetics* / Q000382:microbiology*; D012463:Saliva; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D005647:Fucosyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase