title: Biodegradation of N,N-dimethylacetamide by Rhodococcus sp. strain B83 isolated from the rhizosphere of pagoda tree.
abstract: The biodegradation characteristic and potential metabolic pathway for removal of environmental N,N-dimethylacetamide (DMAC) by Rhodococcus sp. strain B83 was studied. Rhodococcus sp. strain B83 was isolated from the rhizosphere of a pagoda tree and proved capable of utilizing DMAC as sole source of carbon and nitrogen. Batch culture studies showed that strain B83 could tolerate up to 25g/L DMAC and showed distinct growth on possible catabolic intermediates except for acetate. The nitrogen balance analysis revealed that approximately 71% of the initial nitrogen was converted to organic nitrogen. DMAC degradation has led to accumulation of acetate and organic nitrogen, meanwhile traces of nitrate and ammonia was build-up but without nitrite. The growth of strain B83 could be inhibited by adding exogenous acetate. By means of the assay of enzymatic degradation of DMAC, several catabolic intermediates at different intervals were observed and identified. Based on the results obtained from culture solution and enzymatic degradation assay, a detailed pathway is proposed for DMAC biodegradation.
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: chenxingdu123@163.com.
authors.forename: Xingdu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Chengjian
authors.initials: C
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Bizhou
authors.initials: B
authors.lastname: Ge
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Yucan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Nan
mesh_terms: D000081:Acetamides / Q000378:metabolism*; D001673:Biodegradation, Environmental; D007887:Fabaceae / Q000382:microbiology*; D058441:Rhizosphere; D012240:Rhodococcus / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D000081:Acetamides; C013959:dimethylacetamide
keywords: Biodegradation pathway; Catabolic intermediates; N,N-dimethylacetamide; Rhodococcus sp. strain B83