title: Effects of melatonin on maturation, histone acetylation, autophagy of porcine oocytes and subsequent embryonic development.
abstract: Melatonin (MLT) is an endogenous hormone with roles in animal germ cell development. However, the effect of MLT on porcine oocyte maturation and its underlying mechanisms remain largely unknown. Here, we investigated the effects of exogenous MLT on oocyte maturation, histone acetylation, autophagy and subsequent embryonic development. We found that 1 nmol/L MLT supplemented in maturation medium was the optimal concentration to promote porcine oocyte maturation and subsequent developmental competence and quality of parthenogenetic embryos. Interestingly, the beneficial effects of 1 nmol/L MLT treatment on porcine oocyte maturation and embryo development were mainly attributed to the first half period of in vitro maturation. Simultaneously, MLT treatment could also improve maturation of small follicle-derived oocytes, morphologically poor (cumulus cell layer ≤1) and even artificially denuded oocytes and their subsequent embryo development. Furthermore, MLT treatment not only could decrease the levels of H3K27ac and H4K16ac in metaphase II (MII) oocytes, but also could increase the expression abundances of genes associated with cumulus cell expansion, meiotic maturation, histone acetylation and autophagy in cumulus cells or MII oocytes. These results indicate that MLT treatment can facilitate porcine oocyte maturation and subsequent embryonic development probably, through improvements in histone acetylation and autophagy in oocytes.
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Renyun
authors.initials: R
authors.lastname: Hong
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Ding
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Chengxue
authors.initials: C
authors.lastname: Liu
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Di
authors.initials: D
authors.lastname: Gao
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shang
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Zubing
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Huang
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui province, China.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000107:Acetylation / Q000187:drug effects*; D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002478:Cells, Cultured; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D006657:Histones / Q000378:metabolism*; D059471:In Vitro Oocyte Maturation Techniques; D008550:Melatonin / Q000494:pharmacology*; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D013268:Stimulation, Chemical; D013552:Swine
chemical_list: D006657:Histones; D008550:Melatonin
keywords: autophagy; histone acetylation; in vitro maturation; melatonin; porcine oocyte