title: Transcriptional Activity of Gene Encoding Subunits R1 and R2 of Interferon Gamma Receptor in Peripheral Blood Mononuclear Cells in Patients with Slow Coronary Flow.
abstract: BACKGROUND
  Slow coronary flow (SCF) is a coronary artery disorder characterized with delayed opacification of epicardial coronary arteries without obstructive coronary disease. The pathophysiological mechanisms of SCF remain unclear. One of the possible mechanisms that may participate in the pathology of SCF is endothelial dysfunction related to the inflammatory process. Interferon gamma (IFN-γ) is an inflammatory cytokine that acts through its specific receptor composed of two subunits, IFN-γR1 and IFN-γR2. Transcriptional activity of the gene encoding these subunits influences IFN-γ activity. This study aimed to investigate the gene expression of IFN-γ receptor subunits in peripheral blood mononuclear cells (PBMC) from patients with SCF.
  
  
  METHODS
  The study was performed with 30 patients (22 male/8 female) aged 35-76 (52.8±11.7 years) with SCF and 15 sex- (11 male/4 female), Body Max Index (BMI)- and age-matched (54.73±9.42 years) healthy subjects. Total mRNA was extracted from PBMC and was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The relative expression values (2-ΔΔCt) between control and case groups were determined and the Mann-WhitneyUtest was used for statistical analysis.
  
  
  RESULTS
  There was a significant increase in the gene expression of IFN-γR1 in PBMC from SCF patients vs. controls (P< 0.0001); but the differences in IFN-γR2 gene expression were statistically insignificant between patient and control groups (P= 0.853).
  
  
  CONCLUSIONS
  It can be concluded that IFN-γ gene expression may influence the function of microvasculature and thereby contribute to the pathophysiology of SCF.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Faramarz-Gaznagh
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran; Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Rasmi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Mohammad-Hasan
authors.initials: MH
authors.lastname: Khadem-Ansari
authors.affiliation: Department of Cardiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Mir-Hossein
authors.initials: MH
authors.lastname: Seyed-Mohammadzad
authors.affiliation: Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Bagheri
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Mohadeseh
authors.initials: M
authors.lastname: Nemati
authors.affiliation: Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Shirpoor
authors.affiliation: Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Saboori
keywords: coronary artery disease; gene expression; inflammation; interferon gamma receptor; slow coronary flow