title: Bioconversion of Welan Gum from Kitchen Waste by a Two-Step Enzymatic Hydrolysis Pretreatment.
abstract: Kitchen waste (KW) is a worldwide issue, which can lead to environment pollution. Nevertheless, it is also a low-cost and sustainable resource for bio-production. Meanwhile, welan gum (WG) is one kind of the most important exopolysaccharide but with high material cost. The aim of this study was to adopt two-step enzymatic hydrolysis to improve the release and recovery of both sugar and protein in KW for subsequent WG production. As the results, the recovery rates of sugar and protein reached 81.07 and 77.38%, which were both satisfactory. After the conditions optimized in flasks, the welan fermentation was conducted in a 5-L fermentor, and the WG yield, utilization rates of reducing sugar and KDN, respectively, reached 5.57 g L-1, 94.25% and 61.96%. Moreover, the kinetic analyses demonstrated that the WG fermentation in KWH was a partly growth-associated process. The KW was successfully treated by fermentation for the bioconversion to WG.
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Panyu
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Wanrong
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Yabo
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China.
authors.forename: Tonghui
authors.initials: T
authors.lastname: Xie
authors.affiliation: Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China. zhangyongkui@scu.edu.cn.
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D003296:Cooking; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D020578:Sphingomonas / Q000378:metabolism; D014866:Waste Products; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D014866:Waste Products; C068570:welan; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Biomass conversion; Enzyme catalysis; Kitchen waste; Waste utilization; Welan gum