title: Human umbilical cord derived matrix: A scaffold suitable for tissue engineering application.
abstract: BACKGROUND
  Human tissue derived natural extracellular matrix (ECM) has great potential in tissue engineering.
  
  
  OBJECTIVE
  We sought to isolate extracellular matrix derived from human umbilical cord and test its potential in tissue engineering.
  
  
  METHODS
  An enzymatic method was applied to isolate and solubilized complete human umbilical cord derived matrix (hUCM). The obtained solution was analyzed for growth factors, collagen and residual DNA contents, then used to coat 2D and 3D surfaces for cell culture application.
  
  
  RESULTS
  The hUCM was successfully isolated with trypsin digestion to acquire a solution containing various growth factors and collagen but no residual DNA. This hUCM solution can form a coating on 2D and 3D substrates suitable cell culture.
  
  
  CONCLUSIONS
  We developed a new matrix derived from human source that can be further used in tissue engineering.
authors.affiliation: UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.
authors.forename: Pan
authors.initials: P
authors.lastname: Dan
authors.affiliation: UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.
authors.forename: Émilie
authors.initials: É
authors.lastname: Velot
authors.affiliation: UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Mesure
authors.affiliation: UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Groshenry
authors.affiliation: UMR 7564, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Université de Lorraine, Villers-lès-Nancy, 54600, France.
authors.forename: Jalal
authors.initials: J
authors.lastname: Bacharouche
authors.affiliation: UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.|Unité de Thérapie Cellulaire et Tissulaire, CHU de Nancy, Vandœuvre-lès-Nancy, 54500, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Decot
authors.affiliation: UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Menu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D004247:DNA / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014470:Umbilical Cord / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen; D004247:DNA
keywords: Human matrix; growth factors; residual DNA; stem cells; tissue engineering