title: Liposomes as a Putative Tool to Investigate NAADP Signaling in Vasculogenesis.
abstract: Nicotinic acid adenine dinucleotide phosphate (NAADP) is the newest discovered intracellular second messengers, which is able to release Ca2+stored within endolysosomal (EL) vesicles. NAADP-induced Ca2+signals mediate a growing number of cellular functions, ranging from proliferation to muscle contraction and differentiation. Recently, NAADP has recently been shown to regulate angiogenesis by promoting endothelial cell growth. It is, however, still unknown whether NAADP stimulates proliferation also in endothelial progenitor cells, which are mobilized in circulation after an ischemic insult to induce tissue revascularization. Herein, we described a novel approach to prepare NAADP-containing liposomes, which are highly cell membrane permeable and are therefore amenable for stimulating cell activity. Accordingly, NAADP-containing liposomes evoked an increase in intracellular Ca2+concentration, which was inhibited by NED-19, a selective inhibitor of NAADP-induced Ca2+release. Furthermore, NAADP-containing liposomes promoted EPC proliferation, a process which was inhibited by NED-19 and BAPTA, a membrane permeable intracellular Ca2+buffer. Therefore, NAADP-containing liposomes stand out as a promising tool to promote revascularization of hypoxic/ischemic tissues by favoring EPC proliferation. J. Cell. Biochem. 118: 3722-3729, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Bioscience and Territory (DIBT), University of Molise, Contrada Lappone Pesche, Isernia 86090, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Di Nezza
authors.affiliation: Laboratory of General Physiology, Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia 27100, Italy.
authors.forename: Estella
authors.initials: E
authors.lastname: Zuccolo
authors.affiliation: Biotechnology Research Laboratory, Center for the Study of Myelofibrosis, Fondazione IRCCS Policlinico San Matteo, Pavia 27100, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Poletto
authors.affiliation: Biotechnology Research Laboratory, Center for the Study of Myelofibrosis, Fondazione IRCCS Policlinico San Matteo, Pavia 27100, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Rosti
authors.affiliation: Department of Mental and Physical Health and Preventive Medicine, Section of Human Anatomy, Università degli Studi della Campania "L. Vanvitelli", Naples 80138, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: De Luca
authors.affiliation: Laboratory of General Physiology, Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia 27100, Italy.
authors.forename: Francesco
authors.identifier: 0000-0003-0010-0098
authors.initials: F
authors.lastname: Moccia
authors.affiliation: Department of Medicine and Health Sciences "Vincenzo Tiberio", Centre of Nanomedicine, University of Molise, Campobasso 86100, Italy.
authors.forename: Germano
authors.initials: G
authors.lastname: Guerra
authors.affiliation: Department of Medicine and Health Sciences "Vincenzo Tiberio", Centre of Nanomedicine, University of Molise, Campobasso 86100, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosone
mesh_terms: D000328:Adult; D020013:Calcium Signaling / Q000187:drug effects*; D002243:Carbolines / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008081:Liposomes; D008297:Male; D009249:NADP / Q000031:analogs & derivatives* / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010879:Piperazines / Q000378:metabolism
chemical_list: D002243:Carbolines; D008081:Liposomes; C000614918:Ned-19 compound; D010879:Piperazines; D004533:Egtazic Acid; D009249:NADP; C024376:NAADP; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
keywords: Ca2+ SIGNALING; ENDOTHELIAL PROGENITOR CELLS; LIPOSOMES; NAADP; VASCULOGENESIS