title: Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates.
abstract: Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Jinrui
authors.initials: J
authors.lastname: Gan
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Gili
authors.initials: G
authors.lastname: Ben-Nissan
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Galina
authors.initials: G
authors.lastname: Arkind
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Mark
authors.initials: M
authors.lastname: Tarnavsky
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Devin
authors.initials: D
authors.lastname: Trudeau
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Lianet
authors.initials: L
authors.lastname: Noda Garcia
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Dan S
authors.initials: DS
authors.lastname: Tawfik
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
authors.forename: Michal
authors.identifier: 0000-0003-3933-0595
authors.initials: M
authors.lastname: Sharon
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D000075663:Ion Mobility Spectrometry; D055550:Protein Stability; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry*; D012995:Solubility
chemical_list: D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C531130:CBR3 protein, human