title: Pathogenic potentials of Aeromonas species isolated from aquaculture and abattoir environments.
abstract: The present study elucidated the presence of antibiotics resistance, virulence genes and biofilm potentials among Aeromonas species isolated from abattoir and aquaculture environments in Benin City, Nigeria. A total of 144 wastewater samples were obtained from two independent aquaculture and abattoir environments between May and October 2016. Aeromonas species were isolated on Glutamate Starch Phenol Red (GSP) agar and confirmed using API 20NE kits. Antimicrobial susceptibility profile of the isolates was carried out using standard disc diffusion assay while biofilm potentials were detected by the microtitre plate method and PCR technique was used to detect antibiotics resistance and virulence gene markers. Overall, 32 and 26 Aeromonas species were isolated from the abattoir and aquaculture environments respectively. Isolates from both environments were completely resistant (100%) to penicillin G, ertapenem and tetracycline; whereas aquaculture isolates exhibited absolute sensitivity (100%) towards cefepime. All the virulence gene markers (aerA, hlyA, alt, ast, laf, ascF-G, fla, lip, stx1, and stx2) investigated in this study (except laf) were detected in isolates from both environments. The laf genes were only detected in isolates from abattoir environments. Antibiotics resistant genes including pse, blaTEMand class 1 integron were detected in isolates from both environments. Majority of the isolates (53/58 91.4%) from both environments; demonstrated capacity for biofilm potential. The detection of antibiotic resistance and virulence gene markers as well as biofilm forming ability in Aeromonas species isolated from aquaculture and abattoir environments raise serious public health concern worthy of further investigation.
authors.affiliation: Department of Environmental Management and Toxicology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154 Benin City 300001, Nigeria; SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Isoken H
authors.initials: IH
authors.lastname: Igbinosa
authors.affiliation: Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154 Benin City 300001, Nigeria.
authors.forename: Abeni
authors.initials: A
authors.lastname: Beshiru
authors.affiliation: Environmental, Public Health and Bioresource Microbiology Research Group, Department of Basic Sciences, Benson Idahosa University, PMB 1100, Benin City, Nigeria.
authors.forename: Emmanuel E
authors.initials: EE
authors.lastname: Odjadjare
authors.affiliation: Department of Biological Sciences, Microbiology Division, Faculty of Agriculture Science and Technology, North West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2375 South Africa.
authors.forename: Collins N
authors.initials: CN
authors.lastname: Ateba
authors.affiliation: SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa; Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154 Benin City 300001, Nigeria. Electronic address: eigbinosa@gmail.com.
authors.forename: Etinosa O
authors.initials: EO
authors.lastname: Igbinosa
mesh_terms: D000003:Abattoirs; D000333:Aeromonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017756:Aquaculture; D018441:Biofilms / Q000254:growth & development; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D018093:Gelatinases / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D009549:Nigeria; D010447:Peptide Hydrolases / Q000032:analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics; D062065:Wastewater / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018093:Gelatinases; D005819:Genetic Markers; D010447:Peptide Hydrolases; D037521:Virulence Factors; D062065:Wastewater
keywords: Abattoir; Aeromonas; Antimicrobials; Aquaculture; Biofilm; Virulence