title: Experimental and bioinformatic characterization of CaBP2933 an EF-Hand protein of Giardia intestinalis.
abstract: Giardia intestinalis is a parasite that inhabits the small intestine of humans. This parasite is a divergent eukaryote with a compact genome. The calcium ion is an essential messenger in cell signaling. Calcium's role as a messenger is mediated through calcium-binding proteins (CaBPs) that decode the message. The most important family of CaBPs is the EF-Hand protein family. In this study we have explored the role of EF-Hand protein CaBP2933. We analyzed its location, confirmed its ability to bind calcium and identified some of its interacting proteins. Take together our results suggest that CaBP2933 is involved in vesicular trafficking during encystation, via an interaction with kinesin-3 motor protein.
authors.affiliation: Laboratorio de Investigaciones Básicas en Bioquímica- LIBBIQ, Universidad Nacional de Colombia, Calle 44 No. 45-67, Bogotá, Colombia. Electronic address: mealvaradom@unal.edu.co.
authors.forename: Magda E
authors.initials: ME
authors.lastname: Alvarado
authors.affiliation: Laboratorio de Investigaciones Básicas en Bioquímica- LIBBIQ, Universidad Nacional de Colombia, Calle 44 No. 45-67, Bogotá, Colombia.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rubiano
authors.affiliation: Laboratorio de Investigaciones Básicas en Bioquímica- LIBBIQ, Universidad Nacional de Colombia, Calle 44 No. 45-67, Bogotá, Colombia.
authors.forename: Eliana
authors.initials: E
authors.lastname: Calvo
authors.affiliation: Laboratorio de Investigaciones Básicas en Bioquímica- LIBBIQ, Universidad Nacional de Colombia, Calle 44 No. 45-67, Bogotá, Colombia.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Gómez
authors.affiliation: Laboratorio de Investigaciones Básicas en Bioquímica- LIBBIQ, Universidad Nacional de Colombia, Calle 44 No. 45-67, Bogotá, Colombia.
authors.forename: Moisés
authors.initials: M
authors.lastname: Wasserman
mesh_terms: D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D019295:Computational Biology; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D020832:EF Hand Motifs; D016829:Giardia lamblia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D015800:Protozoan Proteins; D016547:Kinesins; D002118:Calcium
keywords: Calcium; Calcium-binding protein; EF-Hand; Encystation; Giardia intestinalis; Kinesin