title: Simultaneous determination of 4-hydroxyphenyl lactic acid, 4-hydroxyphenyl acetic acid, and 3,4-hydroxyphenyl propionic acid in human urine by ultra-high performance liquid chromatography with fluorescence detection.
abstract: A simple and reliable method was established for simultaneous determination of 4-hydroxyphenyl acetic acid, 4-hydroxyphenyl lactic acid, and 3,4-hydroxyphenyl propionic acid in human urine by high-performance liquid chromatography with fluorescence detection. Solid-phase extraction was used to eliminate the interferences in urine. The separation of three analytes was achieved using a C18column and a mobile phase formed by a 95:5 v/v mixture of 50 mmol/L ammonium acetate buffer at pH 6.8 that contained 5 mmol/L tetrabutyl ammonium bromide and acetonitrile. Under the optimized conditions, the detection limits of 4-hydroxyphenyl acetic acid, 4-hydroxyphenyl lactic acid, and 3,4-hydroxyphenyl propionic acid were 4.8 × 10-3, 8.80 × 10-3, and 9.00 × 10-3mg/L, respectively, and the recoveries were in the range of 85.0-120.0% with relative standard deviations of 1.5-3.1%. This method was used to analyze urine samples from breast cancer patients, healthy people and post-surgery breast cancer patients. Significant differences in urinary levels of 4-hydroxyphenyl acetic acid and 4-hydroxyphenyl lactic acid could be found between the breast cancer patients group and other two groups. No effect of age and sex was observed on the urinary levels of 4-hydroxyphenyl acetic acid and 4-hydroxyphenyl lactic acid. This method might be helpful for cancer biomarkers discovery in urine.
authors.affiliation: College of Chemistry, Nanchang University, Nanchang, Jiangxi, P. R. China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Center of Analysis and Testing, Nanchang University, Nanchang, Jiangxi, P. R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Chemistry, Nanchang University, Nanchang, Jiangxi, P. R. China.|Center of Analysis and Testing, Nanchang University, Nanchang, Jiangxi, P. R. China.
authors.forename: Yiqun
authors.identifier: http://orcid.org/0000-0002-8705-2289
authors.initials: Y
authors.lastname: Wan
mesh_terms: D000085:Acetates / Q000652:urine*; D001943:Breast Neoplasms / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000652:urine*; D057230:Limit of Detection; D008297:Male; D011422:Propionates / Q000652:urine*; D052616:Solid Phase Extraction
chemical_list: D000085:Acetates; D011422:Propionates; D019344:Lactic Acid
keywords: biomarkers; breast cancer; high-performance liquid chromatography; urine