title: [Effect of the mediation of the taurine on striatum tissue Ca(2+) homeostasis in manganese exposed rats].
abstract: Objective:To elucidate the effect of taurine on neurotoxicity induced by Mn by investigating activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase and content of Mn and active calmodulin in manganese exposed rats.Methods:156 male SD rats were randomly divided into 1 control group, 3 manganese exposed groups (10, 15, and 20 mg/kg respectively) , and 9 taurine intervened groups based on orthogonal design (doses of taurine intervention were 100, 150, and 200 mg/kg respectively) , with 12 rats in each group. After 12 weeks of exposure, all rats were decapitated and corpus striatums were removed, activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase and content of Mn and active calmodulin were analyzed.Results:The corpus striatum Mn content of the 3 dose groups exposed to Mn and 9 taurine intervened groups were significantly higher than that of the control group (P<0.05) . Active calmodulin content in 10 mg/kg manganese exposed group was significantly higher than that of the control group (P<0.05) . 150 and 200 mg/kg of taurine could decrease active calmodulin content of the group exposed to 10 mg/kg of Mn (P<0.05) . The corpus striatum activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase of the 3 dose groups exposed to Mn were significantly lower than that of the control group (P<0.05) . 150 mg/kg of taurine could increase activities of Na(+)-K(+)-ATPase of the group exposed to 10 mg/kg of Mn (P<0.05) . 150 and 200 mg/kg of taurine could respectively improve activities of Ca(2+)-Mg(2+)-ATPase of the group exposed to 15, 10 mg/kg of Mn (P<0.05) .Conclusion:Mn can decrease the rats corpus striatum activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, effect level of active calmodulin in relation to dose of Mn, to a certain extent, taurine could regulate activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase and improve the level of active calmodulin.
authors.affiliation: Department of Phylaxiology, Xiamen Medical College, Xiamen 361023, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Qin
authors.forename: S C
authors.initials: SC
authors.lastname: Guo
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008345:Manganese / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase; D013654:Taurine / Q000378:metabolism*
chemical_list: D013654:Taurine; D008345:Manganese; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Ca(2+) homeostasis; Corpus striatum; Manganese; Rats; Taurine