title: Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs.
abstract: Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.|Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Long-Jyun
authors.initials: LJ
authors.lastname: Su
authors.affiliation: Department of Pharmacology, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Meng-Shiue
authors.initials: MS
authors.lastname: Wu
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.
authors.forename: Yuen Yung
authors.initials: YY
authors.lastname: Hui
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.
authors.forename: Be-Ming
authors.initials: BM
authors.lastname: Chang
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Surgery, College of Medicine and the Hospital, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Pei-Chen
authors.initials: PC
authors.lastname: Hsu
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Yit-Tsong
authors.initials: YT
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Hong-Nerng
authors.initials: HN
authors.lastname: Ho
authors.affiliation: Department of Biochemistry and Molecular Cell Biology, School of Medicine, Graduate Institute of Medical Sciences, College of Medicine, Centre for Cell Therapy and Regeneration Medicine, and International PhD Program for Cell Therapy and Regeneration Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Yen-Hua
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Thai-Yen
authors.initials: TY
authors.lastname: Ling
authors.affiliation: Department of Surgery, College of Medicine and the Hospital, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Hsao-Hsun
authors.initials: HH
authors.lastname: Hsu
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.|Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
authors.forename: Huan-Cheng
authors.initials: HC
authors.lastname: Chang
mesh_terms: D000072283:A549 Cells; D000818:Animals; D001672:Biocompatible Materials; D058948:Cell Tracking / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008168:Lung / Q000166:cytology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D058612:Nanodiamonds / Q000008:administration & dosage / Q000737:chemistry*; D000075462:Serum Albumin, Human / Q000008:administration & dosage / Q000737:chemistry; D059629:Signal-To-Noise Ratio; D013552:Swine; D013556:Swine, Miniature; D014018:Tissue Distribution
chemical_list: D001672:Biocompatible Materials; D058612:Nanodiamonds; D000075462:Serum Albumin, Human