title: Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach.
abstract: The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters were substantiated by histological and molecular data. A smear without ladder formation on agarose gel was shown, indicating random DNA degradation in the liver and the kidney of MT treated rats. The increase in cyclooxygenase-2 gene expression, marker of inflammation, and an increase in genes expression of glutathione peroxidase and superoxide dismutase in liver and their decrease in the kidney were also occurred after MT exposure. These data confirmed the pro-oxidant and genotoxic effects of this fungicide.
authors.affiliation: Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Feki
authors.affiliation: Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, 3029 Sfax, University of Sfax , Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Ben Saad
authors.affiliation: Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia.
authors.forename: I
authors.initials: I
authors.lastname: Jaballi
authors.affiliation: EA 2219 Géoarchitecture, University of Western Brittany, UFR Sciences & Techniques, 6 Avenue V. Le Gorgeu, CS 93 837, 29238 Brest Cedex 3, France.
authors.forename: C
authors.initials: C
authors.lastname: Magne
authors.affiliation: Anatomopathology Laboratory, CHU Habib Bourguiba 3029. Sfax University. Tunisia.
authors.forename: O
authors.initials: O
authors.lastname: Boudawara
authors.affiliation: Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, 3029 Sfax, University of Sfax , Tunisia.
authors.forename: K M
authors.initials: KM
authors.lastname: Zeghal
authors.affiliation: Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, 3029 Sfax, University of Sfax , Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Hakim
authors.affiliation: Laboratory of Biochemistry and Enzymatic Engineering of Lipases. BP3038-1173, Sfax University, Tunisia.
authors.forename: Y
authors.initials: Y
authors.lastname: Ben Ali
authors.affiliation: Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia.
authors.forename: I
authors.initials: I
authors.lastname: Ben Amara
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000008:administration & dosage / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013875:Thiophanate / Q000008:administration & dosage / Q000633:toxicity*; D023382:Toxicity Tests, Acute / Q000379:methods*
chemical_list: D005659:Fungicides, Industrial; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013875:Thiophanate; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Genotoxicity; Oxidative stress.; Rat; Thiophanate-methyl