title: Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance.
abstract: Paper mulberry is a valuable woody species with a good chilling tolerance. In this study, phosphoproteomic analysis, physiological measurement, and mRNA quantification were employed to explore the molecular mechanism of chilling (4 °C) tolerance in paper mulberry. After chilling for 6 h, 427 significantly changed phosphoproteins were detected in paper mulberry seedlings without obvious physiological injury. When obvious physiological injury occurred after chilling for 48 h, a total of 611 phosphoproteins were found to be significantly changed at the phosphorylation level. Several protein kinases, especially CKII, were possibly responsible for these changes according to conserved sequence analysis. The results of Gene Ontology analysis showed that phosphoproteins were mainly responsible for signal transduction, protein modification, and translation during chilling. Additionally, transport and cellular component organization were enriched after chilling for 6 and 48 h, respectively. On the basis of the protein-protein interaction network analysis, a protein kinase and phosphatases hub protein (P1959) were found to be involved in cross-talk between Ca2+, BR, ABA, and ethylene-mediated signaling pathways. We also highlighted the phosphorylation of BpSIZ1 and BpICE1 possibly impacted on the CBF/DREB-responsive pathway. From these results, we developed a schematic for the chilling tolerance mechanism at phosphorylation level.
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences , Beijing 100093, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Zhi
authors.identifier: 0000-0002-7301-6893
authors.initials: Z
authors.lastname: Pi
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences , Beijing 100093, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences , Beijing 100093, China.
authors.forename: Xian-Jun
authors.initials: XJ
authors.lastname: Peng
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences , Beijing 100093, China.
authors.forename: Shi-Hua
authors.initials: SH
authors.lastname: Shen
mesh_terms: D000222:Adaptation, Physiological; D003080:Cold Temperature; D063990:Gene Ontology; D031625:Morus / Q000737:chemistry*; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D010940:Plant Proteins / Q000032:analysis*; D060066:Protein Interaction Maps; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D010750:Phosphoproteins; D010940:Plant Proteins
keywords: CBF/DREB-responsive pathway; chilling tolerance; hub protein; paper mulberry; phosphorylation