title: The rice LysM receptor-like kinase OsCERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling.
abstract: The rice lysin-motif (LysM) receptor-like kinase OsCERK1 is now known to have a dual role in both pathogenic and symbiotic interactions. Following the recent discovery that the Oscerk1 mutant is unable to host arbuscular mycorrhizal (AM) fungi, we have examined whether OsCERK1 is directly involved in the perception of the short-chain chitin oligomers (Myc-COs) identified in AM fungal exudates and shown to activate nuclear calcium (Ca2+) spiking in the rice root epidermis. An Oscerk1 knockout mutant expressing the cameleon NLS-YC2.60 was used to monitor nuclear Ca2+signaling following root treatment with either crude fungal exudates or purified Myc-COs. Compared with wild-type rice, Ca2+spiking responses to AM fungal elicitation were absent in root atrichoblasts of the Oscerk1 mutant. By contrast, rice lines mutated in OsCEBiP, encoding the LysM receptor-like protein which associates with OsCERK1 to perceive chitin elicitors of the host immune defense pathway, responded positively to Myc-COs. These findings provide direct evidence that the bi-functional OsCERK1 plays a central role in perceiving short-chain Myc-CO signals and activating the downstream conserved symbiotic signal transduction pathway.
authors.affiliation: Department of Life Sciences and Systems Biology, University of Torino, Torino, 10125, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Carotenuto
authors.affiliation: Laboratory of Plant-Microbe Interactions (LIPM), INRA-CNRS-Toulouse University, Castanet-Tolosan, 31326, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Chabaud
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Miyata
authors.affiliation: Department of Life Sciences and Systems Biology, University of Torino, Torino, 10125, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Capozzi
authors.affiliation: National Institute for Basic Biology (NIBB)/SOKENDAI, Nishigonaka 38, Myodaiji, Okazaki, Aichi, 444-8585, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Takeda
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571, Japan.
authors.forename: Hanae
authors.initials: H
authors.lastname: Kaku
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Shibuya
authors.affiliation: National Institute for Basic Biology (NIBB)/SOKENDAI, Nishigonaka 38, Myodaiji, Okazaki, Aichi, 444-8585, Japan.|Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi, 464-8602, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Laboratory of Plant-Microbe Interactions (LIPM), INRA-CNRS-Toulouse University, Castanet-Tolosan, 31326, France.
authors.forename: David G
authors.initials: DG
authors.lastname: Barker
authors.affiliation: Department of Life Sciences and Systems Biology, University of Torino, Torino, 10125, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Genre
mesh_terms: D002118:Calcium / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D055786:Gene Knockout Techniques; D009154:Mutation; D038821:Mycorrhizae / Q000378:metabolism* / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins; D002686:Chitin; D017346:Protein Serine-Threonine Kinases; D002118:Calcium
keywords: 
  Oryza sativa
  ; LysM receptor-like kinase (RLK); arbuscular mycorrhiza (AM); chitin oligomer signaling; nuclear calcium spiking; plant-microbe interactions; root symbiosis