title: Choerosponins A and B, Two New Cytotoxic Bridged-Ring Ketones and the Determination of Their Absolute Configurations.
abstract: Bioactivity-directed fractionation of antitumor compounds from the stem barks ofChoerospondias axillaries(Roxb.) Burtt et Hill (Anacardiaceae) afforded two new cytotoxic bridged-ring ketones, choerosponins A (1) and B (2), and their structures were elucidated by spectroscopic methods; their stereochemistry was determined by NOE difference experiments, CD spectra and the modified Mosher's method. Compound1has a rare dioxatricyclo skeleton. Flow cytometry and SRB methods were employed to evaluate the antitumor activity of the two compounds against tsFT210, HCT-15, HeLa, A2780 and MCF-7 cell lines, and both of them showed strong cytotoxicity. MTT and paper disc methods were also used to evaluate their anti-hypoxia and antibacterial activities, and both of them showed no apparent activities.
authors.affiliation: State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. sdrlcw@126.com.
authors.forename: Chang-Wei
authors.initials: CW
authors.lastname: Li
authors.affiliation: Beijing Institute for Biomedicinal Research, Beijing 100091, China. h3b3@sohu.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Beijing Institute for Biomedicinal Research, Beijing 100091, China. caibing05@126.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. cuicb@126.com.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Cui
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D015687:Cell Hypoxia / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007659:Ketones / Q000737:chemistry* / Q000494:pharmacology*; D061986:MCF-7 Cells; D015394:Molecular Structure
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D007659:Ketones
keywords: Choerospondias axillaries; absolute configuration; bridged ring ketones; cytotoxicity