title: Combination therapy with catechins and caffeine inhibits fat accumulation in 3T3-L1 cells.
abstract: Catechins and caffeine, which are green tea components, have a slimming effect; however, the combinational effect of fat metabolism in 3T3-L1 cells remains unclear. In the present study, 3T3-L1 cells were treated with catechins and caffeine in combination, and it was found that combination therapy with catechins and caffeine markedly reduced intracellular fat accumulation, mRNA expression levels of peroxisome proliferator-activated receptor-γ and CCAAT/enhancer-binding protein α in the early stage of cell differentiation were significantly reduced, and mRNA expression of fatty acid synthetase(FAS) andglycerol-3-phosphate dehydrogenase protein expression levels of FAS were downregulated. Noradrenaline-induced lipolysis was enhanced by caffeine, which markedly increased the protein expression of adipose triglyceride lipase and hormone sensitive lipase. These results indicated that combination therapy with catechins and caffeine synergistically inhibited lipid accumulation by regulating the gene and protein expression levels of lipid metabolism-related enzymes. Therefore, catechins and caffeine combination therapy has potential as a functional food that may be used to prevent obesity and lifestyle-associated diseases.
authors.affiliation: Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P.R. China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P.R. China.
authors.forename: Licong
authors.initials: L
authors.lastname: Yang
authors.affiliation: Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P.R. China.
authors.forename: Lezhen
authors.initials: L
authors.lastname: Lin
authors.affiliation: Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P.R. China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zheng
keywords: 3T3-L1; caffeine; catechins; lipogenesis; lipolysis