title: A comparison between constitutive and inducible transgenic expression of the PhRIP I gene for broad-spectrum resistance against phytopathogens in potato.
abstract: OBJECTIVE
  To engineer broad spectrum resistance in potato using different expression strategies.
  
  
  RESULTS
  The previously identified Ribosome-Inactivating Protein from Phytolacca heterotepala was expressed in potato under a constitutive or a wound-inducible promoter. Leaves and tubers of the plants constitutively expressing the transgene were resistant to Botrytis cinerea and Rhizoctonia solani, respectively. The wound-inducible promoter was useful in driving the expression upon wounding and fungal damage, and conferred resistance to B. cinerea. The observed differences between the expression strategies are discussed considering the benefits and features offered by the two systems.
  
  
  CONCLUSIONS
  Evidence is provided of the possible impact of promoter sequences to engineer BSR in plants, highlighting that the selection of a suitable expression strategy has to balance specific needs and target species.
authors.affiliation: Dipartimento di Agraria, Università degli Studi di Napoli "Federico II", Portici, NA, Italy.
authors.forename: Romel
authors.initials: R
authors.lastname: Gonzales-Salazar
authors.affiliation: Dipartimento di Agraria, Università degli Studi di Napoli "Federico II", Portici, NA, Italy.
authors.forename: Bianca
authors.initials: B
authors.lastname: Cecere
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, CNR, Portici, NA, Italy.
authors.forename: Michelina
authors.initials: M
authors.lastname: Ruocco
authors.affiliation: Dipartimento di Agraria, Università degli Studi di Napoli "Federico II", Portici, NA, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Rao
authors.affiliation: Dipartimento di Agraria, Università degli Studi di Napoli "Federico II", Portici, NA, Italy. giandomenico.corrado@unina.it.
authors.forename: Giandomenico
authors.initials: G
authors.lastname: Corrado
mesh_terms: D020171:Botrytis / Q000276:immunology / Q000472:pathogenicity; D060467:Disease Resistance; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000276:immunology*; D029603:Phytolacca / Q000201:enzymology / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012232:Rhizoctonia / Q000276:immunology / Q000472:pathogenicity; D054788:Ribosome Inactivating Proteins / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000276:immunology*
chemical_list: D011994:Recombinant Proteins; D054788:Ribosome Inactivating Proteins
keywords: Fungal resistance; Potato; Ribosome-inactivating protein; Solanum tuberosum; Transgenic plants