title: Allogeneic islet cells implant on poly-l-lactide matrix to reduce hyperglycaemia in streptozotocin-induced diabetic rat.
abstract: OBJECTIVE
  To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes.
  
  
  METHODS
  Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-(l-lactide) matrices. Animals were rendered diabetic and a week later a matrix seeded with islet cells (IMI group) or a control matrix (placebo group) was implanted in the small bowel mesentery. Blood glucose levels were measured weekly for 12 weeks. After sacrifice, implant sections were Gomori stained for beta-cells and immuno-stained for insulin 3, 4, 5, and 6 months post implantation.
  
  
  RESULTS
  82% of seeded islet cells attached to the matrices. In the IMI group blood glucose levels were significantly reduced after implantation compared with before implantation across several time points. In the IMI group beta-cells and insulin-positive cells were identified at the implant site.
  
  
  CONCLUSIONS
  The islet cell matrix implant reduced the blood glucose levels although complete normo-glycaemia was not established. The islet cell matrix implant may serve as an additional option for islet cell transplantation using 3D scaffold platforms for better survival and function of the islet cells.
authors.affiliation: Tarumanagara Human Cell Technology Laboratory, Faculty of Medicine, Tarumanagara University, Indonesia; Postgraduate School, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Electronic address: siufui@gmail.com.
authors.forename: Siufui
authors.initials: S
authors.lastname: Hendrawan
authors.affiliation: Department of Physiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Electronic address: irawanyusufgenome@gmail.com.
authors.forename: Irawan
authors.initials: I
authors.lastname: Yusuf
authors.affiliation: Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Electronic address: hattaram@indosat.net.id.
authors.forename: Mochammad
authors.initials: M
authors.lastname: Hatta
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Electronic address: makbul_aman@yahoo.com.
authors.forename: Makbul
authors.initials: M
authors.lastname: Aman
authors.affiliation: Department of Physiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Electronic address: ilham_pt@yahoo.com.
authors.forename: Ilhamjaya
authors.initials: I
authors.lastname: Patellongi
authors.affiliation: Epidemiology, Biostatistics, and Prevention Institute, University of Zurich, Switzerland. Electronic address: andreas.serra@uzh.ch.
authors.forename: Andreas L
authors.initials: AL
authors.lastname: Serra
authors.affiliation: Department of Anatomic Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Electronic address: gatot.law@gmail.com.
authors.forename: Gatot
authors.initials: G
authors.lastname: Lawrence
authors.affiliation: Baermed, Center of Abdominal Surgery, Hirslanden Clinic Zurich and University of Bern, Switzerland. Electronic address: uweber159@gmail.com.
authors.forename: Ursula
authors.initials: U
authors.lastname: Weber
authors.affiliation: Department of Surgery, Gading Pluit Hospital, Jakarta, Indonesia. Electronic address: dr.barlian@pluit-hospital.com.
authors.forename: Barlian
authors.initials: B
authors.lastname: Sutedja
authors.affiliation: Baermed, Center of Abdominal Surgery, Hirslanden Clinic Zurich and University of Bern, Switzerland. Electronic address: hans.baer@baermed.ch.
authors.forename: Hans U
authors.initials: HU
authors.lastname: Baer
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D018929:Cell Culture Techniques; D002469:Cell Separation; D002470:Cell Survival; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000473:pathology / Q000628:therapy*; D006943:Hyperglycemia / Q000209:etiology / Q000628:therapy*; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D011091:Polyesters; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D007328:Insulin; D011091:Polyesters; C033616:poly(lactide)
keywords: Diabetes; Glycaemic control; Islet matrix implant; Rat