title: iTRAQ proteomic analysis of the hippocampus in a rat model of nicotine-induced conditioned place preference.
abstract: Repeated exposures to nicotine are known to result in persistent changes in proteins expression in addiction-related brain regions, such as the striatum, nucleus accumbens and prefrontal cortex, but the changes induced in the protein content of the hippocampus remain poorly studied. This study established a rat model of nicotine-induced conditioned place preference (CPP), and screened for proteins that were differentially expressed in the hippocampus of these rats using isobaric tags for relative and absolute quantitation labeling (iTRAQ) coupled with 2D-LC MS/MS. The nicotine-induced CPP was established by subcutaneously injecting rats with 0.2 mg/kg nicotine. Relative to the control (saline) group, the nicotine group showed 0.67- and 1.5-fold changes in 117 and 10 hippocampal proteins, respectively. These differentially expressed proteins are mainly involved in calcium-mediated signaling, neurotransmitter transport, GABAergic synapse function, long-term synaptic potentiation and nervous system development. Furthermore, RT-PCR was used to confirmed the results of the proteomic analysis. Our findings identify several proteins and cellular signaling pathways potentially involved in the molecular mechanisms in the hippocampus that underlie nicotine addiction. These results provide insights into the mechanisms of nicotine treatment in hippocampus.
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhu
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Li
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China.
authors.forename: Xinchao
authors.initials: X
authors.lastname: Zhu
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China. Electronic address: qsfctc@163.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Hou
authors.affiliation: China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China. Electronic address: huqy1965@163.com.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005108:Extinction, Psychological / Q000187:drug effects / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009538:Nicotine / Q000008:administration & dosage*; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods; D014018:Tissue Distribution
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome; D009538:Nicotine
keywords: Conditioned place preference; Hippocampus; Nicotine; Proteomics; iTRAQ