title: Method for detection of hydrogen peroxide in HT22 cells.
abstract: We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C2O4)(pm)(OH2)2]+(pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models.
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jacewicz
authors.affiliation: Department of Histology, Medical University of Gdansk, Debinki 1, 80-211, Gdansk, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Siedlecka-Kroplewska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Drzeżdżon
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piotrowska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Wyrzykowski
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Tesmar
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Żamojć
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Chmurzyński
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D006624:Hippocampus / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D018384:Oxidative Stress
chemical_list: D006861:Hydrogen Peroxide