title: A rapid analytical and quantitative evaluation of formaldehyde in squid based on Tri-step IR and partial least squares (PLS).
abstract: Formaldehyde abuse for retaining squid freshness is detrimental to public health. The aim is to establish a rapid and quantitative detection method of formaldehyde in squid for screening massive samples. A linear relationship between formaldehyde concentration in squid and formaldehyde concentration in squid soaked water was observed using HPLC. Chemical profile variances of squids were rapidly analyzed by Tri-step infrared spectroscopy. Specifically, with increasing formaldehyde concentration, peak intensities of 2927cm-1(vas(CH2)), 1081cm-1(glycogen), 1631cm-1(β-sheet proteins) decreased while 1655cm-1(α-helix proteins) increased. Spectral curve-fitting results further disclosed that β-sheet proteins were transformed to α-helix and β-turn conformations. Furthermore, a quantitative prediction model based on IR spectra was established by PLS (R2, 0.9787; RMSEC, 5.51). The developed method was applicable for rapid (c.a. 5min) and quantitative analysis of formaldehyde in squids with LOD of 15mg/kg.
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing Preservation, Shanghai 201306, PR China.
authors.forename: Dong-Chen
authors.initials: DC
authors.lastname: Gu
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Meng-Jun
authors.initials: MJ
authors.lastname: Zou
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing Preservation, Shanghai 201306, PR China.
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Guo
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Lin
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Tu
authors.initials: T
authors.lastname: Hu
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Ya-Fen
authors.initials: YF
authors.lastname: Wu
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing Preservation, Shanghai 201306, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Gan
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Su-Qin
authors.initials: SQ
authors.lastname: Sun
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing Preservation, Shanghai 201306, PR China. Electronic address: xcwang@shou.edu.cn.
authors.forename: Xi-Chang
authors.initials: XC
authors.lastname: Wang
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing Preservation, Shanghai 201306, PR China. Electronic address: chxu@shou.edu.cn.
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Xu
mesh_terms: D000818:Animals; D049832:Decapodiformes / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D005557:Formaldehyde / Q000032:analysis*; D016018:Least-Squares Analysis; D057230:Limit of Detection; D013055:Spectrophotometry, Infrared / Q000379:methods*
chemical_list: D005557:Formaldehyde
keywords: Formaldehyde; Partial least squares; Quantitative detection model; Spectral curve-fitting analysis; Squid (Illex argentinus); Tri-step infrared spectroscopy