title: A new immunosuppressive pregnane glycoside from aqueous fraction of Epigynum cochinchinensis.
abstract: Chemical investigation on the aqueous fraction of the stems of Epigynum cochinchinensis led to the isolation of a new pregnane glycoside named as epigycoside A (1) along with three known analogues (2-4). The structure of compound 1 was elucidated by means of spectroscopic techniques, including HRESIMS, and 1D and 2D NMR experiments. The immunosuppressive activity of 1 was evaluated by an in vitro model of concanavalin A-induced mice splenocytes proliferation. Compound 1 showed significant inhibitory activity in a dose-dependent manner, closer to the efficacy of positive control, dexamethasone, at a concentration of 50 μM.
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Zong
authors.initials: Z
authors.lastname: Wan
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Yuan-Cheng
authors.initials: YC
authors.lastname: Yao
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Sheng-Bao
authors.initials: SB
authors.lastname: Cai
authors.affiliation: b Department of Chemistry , COMSATS Institute of Information Technology , Abbottabad , Pakistan.
authors.forename: Afsar
authors.initials: A
authors.lastname: Khan
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Tian-Rui
authors.initials: TR
authors.lastname: Zhao
authors.affiliation: c Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , People's Republic of China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fan
authors.affiliation: d Guizhou Key Laboratory of Characteristic Microbial Research & Drug Development , Zunyi Medical University , Zunyi , People's Republic of China.
authors.forename: Sheng-Yan
authors.initials: SY
authors.lastname: Qian
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Cao
authors.affiliation: a Kunming University of Science and Technology , Yunnan Institute of Food Safety , Kunming , People's Republic of China.
authors.forename: Gui-Guang
authors.initials: GG
authors.lastname: Cheng
mesh_terms: D000818:Animals; D029062:Apocynaceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
chemical_list: D006027:Glycosides; D007166:Immunosuppressive Agents; D011278:Pregnanes; C574437:pregnane glycoside
keywords: Epigynum cochinchinensis; epigycoside A; immunosuppressive; pregnane glycosides