title: Functions and regulation of the MRX complex at DNA double-strand breaks.
abstract: DNA double-strand breaks (DSBs) pose a serious threat to genome stability and cell survival. Cells possess mechanisms that recognize DSBs and promote their repair through either homologous recombination (HR) or non-homologous end joining (NHEJ). The evolutionarily conserved Mre11-Rad50-Xrs2 (MRX) complex plays a central role in the cellular response to DSBs, as it is implicated in controlling end resection and in maintaining the DSB ends tethered to each other. Furthermore, it is responsible for DSB signaling by activating the checkpoint kinase Tel1 that, in turn, supports MRX function in a positive feedback loop. The present review focuses mainly on recent works in the budding yeast Saccharomyces cerevisiae to highlight structure and regulation of MRX as well as its interplays with Tel1.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Gobbini
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
authors.forename: Corinne
authors.initials: C
authors.lastname: Cassani
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Villa
authors.affiliation: Institute of Molecular Biology gGmbH (IMB), 55128 Mainz, Germany.
authors.forename: Diego
authors.initials: D
authors.lastname: Bonetti
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Longhese
keywords: MRX; Rif2; Sae2; Tel1; double-strand break; nucleases; resection