title: Multi-Omics Analysis Reveals a Correlation between the Host Phylogeny, Gut Microbiota and Metabolite Profiles in Cyprinid Fishes.
abstract: Gut microbiota play key roles in host nutrition and metabolism. However, little is known about the relationship between host genetics, gut microbiota and metabolic profiles. Here, we used high-throughput sequencing and gas chromatography/mass spectrometry approaches to characterize the microbiota composition and the metabolite profiles in the gut of five cyprinid fish species with three different feeding habits raised under identical husbandry conditions. Our results showed that host species and feeding habits significantly affect not only gut microbiota composition but also metabolite profiles (ANOSIM,p≤ 0.05). Mantel test demonstrated that host phylogeny, gut microbiota, and metabolite profiles were significantly related to each other (p≤ 0.05). Additionally, the carps with the same feeding habits had more similarity in gut microbiota composition and metabolite profiles. Various metabolites were correlated positively with bacterial taxa involved in food degradation. Our results shed new light on the microbiome and metabolite profiles in the gut content of cyprinid fishes, and highlighted the correlations between host genotype, fish gut microbiome and putative functions, and gut metabolite profiles.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of SciencesWuhan, China; Key Laboratory of Environmental and Applied Microbiology, and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of SciencesChengdu, China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Li
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences Wuhan, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Long
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, and Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences Chengdu, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Nutrition, Métabolisme et Aquaculture, Institut National de la Recherche Agronomique, University of Pau and Pays de l'Adour Saint-Pée-sur-Nivelle, France.
authors.forename: François-Joël
authors.initials: FJ
authors.lastname: Gatesoupe
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University Shanghai, China.
authors.forename: Xujie
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of SciencesWuhan, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: National Fisheries Technical Extension Center, Ministry of Agriculture Beijing, China.
authors.forename: Dongyue
authors.initials: D
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of SciencesWuhan, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Li
keywords: correlation; cyprinid fishes; gut microbiota; host phylogeny; metabolite profiles