title: Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
abstract: Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges. Surface morphological observations showed that structural combinations and intermolecular interactions occurred between collagen and OMCC in the composites. M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals. The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05). These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis. Furthermore, M2 was not cytotoxic and was completely biodegraded in subcutaneous tissue within 28days.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: lihui881635@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China. Electronic address: chengweilu061031@163.com.
authors.forename: Weilu
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: liukeonline@126.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China. Electronic address: 382440154@qq.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China. Electronic address: ydhuang.hit1@aliyun.com.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: wxf_hrbmu@163.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: lzy_hrbmu@163.com.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lv
authors.affiliation: School of Chemical Engineering and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China. Electronic address: 88553532@qq.com.
authors.forename: Jinmei
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Obstetrics, The Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. Electronic address: leec19@sohu.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D002482:Cellulose / Q000737:chemistry*; D002483:Cellulose, Oxidized; D003094:Collagen / Q000494:pharmacology*; D006487:Hemostasis; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D010314:Partial Thromboplastin Time; D011817:Rabbits; D013918:Thrombin Time
chemical_list: D002483:Cellulose, Oxidized; D006490:Hemostatics; D002482:Cellulose; D003094:Collagen; C109691:microcrystalline cellulose
keywords: Biodegradation; Collagen; Hemostasis; Oxidized microcrystalline cellulose