title: Anti-RhoC siRNAs inhibit the proliferation and invasiveness of breast cancer cells via modulating the KAI1, MMP9, and CXCR4 expression.
abstract: Overexpression of RhoC in breast cancer cells indicates poor prognosis. In the present study, we aim to investigate the possible antitumor effects of anti-RhoC small-interfering RNA (siRNA) in inflammatory breast cancer cells. In this study, a specific anti-RhoC siRNA was used to inhibit RhoC synthesis. Transfection of anti-RhoC siRNA into two IBC cells SUM149 and SUM190 induced extensive degradation of target mRNA and led to significant decrease in the synthesis of protein. Anti-RhoC siRNA inhibited cell proliferation and invasion, increased cell apoptosis, and induced cell cycle arrest in vitro. Moreover, the transfection of siRNA increased the expression of KAI1 and decreased the expression of MMP9 and CXCR4 in both mRNA and protein levels. Furthermore, transplantation tumor experiments in BALB/c-nu mice showed that intratumoral injection of anti-RhoC siRNA inhibited tumor growth and increased survival rate. Our results suggested that RhoC gene silencing with specific anti-RhoC siRNA would be a potential therapeutic method for metastatic breast cancer.
authors.affiliation: Department of Thyroid and Breast Surgery, The Fifth Hospital of Wuhan, Hanyang District.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Xu
authors.affiliation: Department of Thyroid and Breast Surgery, The Fifth Hospital of Wuhan, Hanyang District.
authors.forename: Han-Bin
authors.initials: HB
authors.lastname: Shen
authors.affiliation: Department of Oncology.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Oncology.
authors.forename: Jian-Qin
authors.initials: JQ
authors.lastname: Lu
authors.affiliation: Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Luo
authors.affiliation: Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Ya-Qun
authors.initials: YQ
authors.lastname: Wu
keywords: apoptosis; cell cycle arrest; gene silencing; proliferation