title: The effect of ozone on the expression of metallothionein in tissues of rats chronically exposed to cadmium.
abstract: Our aims were to evaluate the expression of metallothionein (MT) in an experimental rat model which experienced chronic exposure to cadmium (Cd) and to measure its expression after ozone therapy (OT) or oxygen (Ox) in the same model, as compared to the control group, which was exposed to neither cadmium nor ozone. Forty male Wistar rats were divided into 5 groups: control, Cd, Cd and Ox, Cd and Oz, and Oz. During our research, Cd concentration (ASA) and MT concentration (ELISA) were determined in supernatants of the kidneys, liver and pancreas. SDS-PAGE analyses and immunohistochemical localization were used to evaluate the level of MT expression in the tissue. In rats intoxicated with Cd, the highest concentration of both Cd and MT was observed in the kidneys and liver, with a significantly lower concentration measured in the pancreas. Ozone therapy reduces the accumulation of cadmium in the liver and kidneys, resulting in a reduced expression of metallothionein in those tissues.
authors.affiliation: Department of Biomedical and Environmental Analysis, Wrocław Medical University, Borowska 211, Poland. Electronic address: halina.milnerowicz@umed.wroc.pl.
authors.forename: Halina
authors.initials: H
authors.lastname: Milnerowicz
authors.affiliation: Department of Biomedical and Environmental Analysis, Wrocław Medical University, Borowska 211, Poland. Electronic address: mariola.sliwinska-mosson@umed.wroc.pl.
authors.forename: Mariola
authors.initials: M
authors.lastname: Śliwińska-Mossoń
authors.affiliation: Department of Basic Physiotherapy, University School of Physical Education in Wroclaw, Poland. Electronic address: krzysztof.sobiech@awf.wroc.pl.
authors.forename: Krzysztof A
authors.initials: KA
authors.lastname: Sobiech
mesh_terms: D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010126:Ozone / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D017208:Rats, Wistar
chemical_list: D004785:Environmental Pollutants; D002104:Cadmium; D010126:Ozone; D008668:Metallothionein; D010100:Oxygen
keywords: Cadmium; Metallothionein; Oxidative stress; Ozone therapy; Rat