title: Incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in Thailand.
abstract: Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3' RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.
authors.affiliation: Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand.|Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand.|Plant Pathology Department, Hortigenetics Research (S.E. Asia) Limited East-West Seeds, Chiang Mai, 50290, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Cheewachaiwit
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Warin
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
authors.forename: B
authors.initials: B
authors.lastname: Phuangrat
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Rukpratanporn
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
authors.forename: O
authors.initials: O
authors.lastname: Gajanandana
authors.affiliation: Plant Pathology Department, Hortigenetics Research (S.E. Asia) Limited East-West Seeds, Chiang Mai, 50290, Thailand.
authors.forename: C H
authors.initials: CH
authors.lastname: Balatero
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand. orawan@biotec.or.th.
authors.forename: O
authors.identifier: http://orcid.org/0000-0003-4676-7904
authors.initials: O
authors.lastname: Chatchawankanphanich
mesh_terms: D018556:Crops, Agricultural / Q000821:virology; D028464:Cucurbita / Q000821:virology*; D016679:Genome, Viral; D052679:Luteoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D057685:Plant Weeds / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D012324:RNA-Dependent RNA Polymerase
keywords: Cucurbit aphid-borne yellows virus; Luteoviridae; Melon aphid-borne yellows virus; Polerovirus; Recombination; Suakwa aphid-borne yellows virus