title: 3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification.
abstract: A rapid, sensitive, and selective precolumn derivatization method for the simultaneous determination of eight thiophenols using 3-(2-bromoacetamido)-N-(9-ethyl-9H)-carbazol as a labeling reagent by high-performance liquid chromatography with fluorescence detection has been developed. The labeling reagent reacted with thiophenols at 50°C for 50 min in aqueous acetonitrile in the presence of borate buffer (0.10 mol/L, pH 11.2) to give high yields of thiophenol derivatives. The derivatives were identified by online postcolumn mass spectrometry. The collision-induced dissociation spectra for thiophenol derivatives gave the corresponding specific fragment ions at m/z 251.3, 223.3, 210.9, 195.8, and 181.9. At the same time, derivatives exhibited intense fluorescence with an excitation maximum at λex= 276 nm and an emission maximum at λem= 385 nm. Excellent linear responses were observed for all analytes over the range of 0.033-6.66 μmol/L with correlation coefficients of more than 0.9997. Detection limits were in the range of 0.94-5.77 μg/L with relative standard deviations of less than 4.54%. The feasibility of derivatization allowed the development of a rapid and highly sensitive method for the quantitative analysis of trace levels of thiophenols from some rubber products. The average recoveries (n = 3) were in the range of 87.21-101.12%.
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.|Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors.forename: Jinmao
authors.identifier: http://orcid.org/0000-0002-8808-6085
authors.initials: J
authors.lastname: You
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Dou
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Cuihua
authors.initials: C
authors.lastname: Song
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, Shandong, P. R. China.
authors.forename: Xianen
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Hu
authors.affiliation: State key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, P. R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
keywords: fluorescence detection; high-performance liquid chromatography; rubber; thiophenols