title: Interleukin 17A in Patients With Stable Coronary Artery Disease: Are There Differences According to Gender?
abstract: BACKGROUND
  Coronary artery disease (CAD) is a current major public health concern. Immunity and inflammation are involved in all phases of CAD and there is a dynamic balance between cells and molecules. Interleukin 17A (IL17A) concentrations are higher in male patients with acute myocardial infarction than in women. In this study, we evaluated if the IL17A concentrations in male CAD patients (MPs) differed from those in female patients (FPs) and male controls (MCs). Moreover, FPs were compared with female controls (FCs).
  
  
  METHODS
  This was a cross-sectional, prospective, and analytical study conducted between March 2012 and August 2013 that enrolled 40 patients (24 men and 16 women) with stable CAD and 20 healthy volunteers (12 men and 8 women) were selected as controls and were matched with the patients (1:2) for sex and age (± 3 years). Comparative analyses of IL17A concentrations in serum and cell culture with and without stimulation were performed between MPs and MCs, MPs and FPs, and FPs and FCs. The lower detection limit was 3.91 pg/mL.
  
  
  RESULTS
  The comparison of the IL17A concentrations showed: after 48 hours of cell culture with stimulus: MP = 451.67 (99.02 - 892.58) vs. MC = 135 (3.91 - 285), P = 0.04; after 48 hours of cell culture with stimulus: MP = 451.67 (99.02 - 892.58) vs. FP = 131.21 (3.91 - 231.97), P = 0.02; after 48 hours of cell culture with stimulus: FP = 131.21 (3.91 - 231.97) vs. FC = 173.78 (3.91 - 642), P = 0.24.
  
  
  CONCLUSIONS
  This study revealed higher IL17A concentrations in the stimulated cells isolated from the MPs than in those isolated from FPs and MCs. These findings support the hypothesis that when exposed to certain stimuli, cells isolated from MPs with chronic CAD may produce higher IL17A concentrations than those from FPs and MCs.
authors.affiliation: Hospital of Clinics, Federal University of Pernambuco, Av. Prof. Moares Rego, 12 35, CEP 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Dinaldo
authors.initials: D
authors.lastname: Oliveira
authors.affiliation: Hospital of Clinics, Federal University of Pernambuco, Av. Prof. Moares Rego, 12 35, CEP 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Elayne
authors.initials: E
authors.lastname: Heide
authors.affiliation: Hospital of Clinics, Federal University of Pernambuco, Av. Prof. Moares Rego, 12 35, CEP 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Brandt
authors.affiliation: Center of Biological Science, Immunomodulation Laboratory and New Therapeutic Approaches, Federal University of Pernambuco, Av. Prof. Moares Rego, 12 35, CEP 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Moacyr
authors.initials: M
authors.lastname: Rego
authors.affiliation: Center of Biological Science, Immunomodulation Laboratory and New Therapeutic Approaches, Federal University of Pernambuco, Av. Prof. Moares Rego, 12 35, CEP 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Maira
authors.initials: M
authors.lastname: Pitta
authors.affiliation: Center of Biological Science, Immunomodulation Laboratory and New Therapeutic Approaches, Federal University of Pernambuco, Av. Prof. Moares Rego, 12 35, CEP 50670-901, Recife, Pernambuco, Brazil.
authors.forename: Ivan
authors.initials: I
authors.lastname: Pitta
keywords: Coronary artery disease; Immunity; Inflammation; Interleukin 17A