title: Rapid G0/1 transition and cell cycle progression in CD8+T cells compared to CD4+T cells following in vitro stimulation.
abstract: T-cell population consists of two major subsets, CD4+T cells and CD8+T cells, which can be distinguished by the expression of CD4 or CD8 molecules, respectively. Although they play quite different roles in the immune system, many of their basic cellular processes such as proliferation following stimulation are presumably common. In this study, we have carefully analyzed time-course of G0/1 transition as well as cell cycle progression in the two subsets of quiescent T-cell population following in vitro growth stimulation. We found that CD8+T cells promote G0/1 transition more rapidly and drive their cell cycle progression faster compared to CD4+T cells. In addition, expression of CD25 and effects of its blockade revealed that IL-2 is implicated in the rapid progression, but not the earlier G0/1 transition, of CD8+T cells.
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Mishima
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Shotaro
authors.initials: S
authors.lastname: Fukaya
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Toda
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ando
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsunaga
authors.affiliation: Laboratory of Human Molecular Genetics, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Inobe
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016193:G1 Phase / Q000235:genetics*; D007376:Interleukin-2 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D016192:Resting Phase, Cell Cycle / Q000235:genetics*
chemical_list: D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit
keywords: G0/1 transition; IL-2; T-cell proliferation; cell cycle