title: Transition Metal-based Anticancer Drugs Targeting Nucleic Acids: A Tandem Mass Spectrometric Investigation.
abstract: The search for effective drugs against cisplatin-resistant tumors resulted in a large number of organometallic compounds that are evaluated for their antiproliferative activity. Among the most promising candidates are bent metallocenes based on various transition metal ions and ligands. The elucidation of structural features and the characterization of the interaction of a drug candidate with its target require accurate and sensitive analytical tools. Tandem mass spectrometry is applied to the investigation of the adduct sites and binding patterns of metallodrugs bound to single-stranded oligonucleotides and higher-order nucleic acids. Results reveal the binding specificities of the different metallodrugs and demonstrate the influence they exert on the dissociation pathways of the adducts in the gas-phase.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern.
authors.forename: Rahel P
authors.initials: RP
authors.lastname: Eberle
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Hari
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern;, Email: stefan.schuerch@dcb.unibe.ch.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schürch
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D002945:Cisplatin / Q000737:chemistry; D018736:DNA Adducts / Q000737:chemistry; D006801:Humans; D000075163:Metallocenes / Q000737:chemistry; D058990:Molecular Targeted Therapy; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D009942:Organometallic Compounds / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*; D028561:Transition Elements
chemical_list: D000970:Antineoplastic Agents; D018736:DNA Adducts; D000075163:Metallocenes; D009696:Nucleic Acids; D009841:Oligonucleotides; D009942:Organometallic Compounds; D028561:Transition Elements; D002945:Cisplatin