title: Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen-thawed Thai native bull sperm.
abstract: This study investigates the effects of iodixanol supplementation in varied concentrations to Tris egg yolk (TEY) extender on the quality and fertilization ability of frozen-thawed sperm of Thai native bulls. Each ejaculate was divided into four different groups, as follows: sperm were treated with TEY extender (control group) and TEY extender supplemented with three different concentrations of iodixanol (1.25%, 2.50% and 5.00%). Semen straws were frozen in liquid nitrogen vapor. After thawing, sperm motility characteristics, viability, plasma membrane integrity and acrosome integrity were determined. Also, frozen-thawed spermatozoa from all groups were used for in vitro fertilization and artificial insemination (AI) in natural estrus Thai native cows. The results showed that the post-thaw quality of the 2.50% iodixanol group was superior to the other iodixanol groups (P < 0.05). However, iodixanol had no beneficial effect on post-thaw sperm in vitro fertilization ability and pregnancy rate after AI (P > 0.05). It can be concluded that the supplementation of 2.50% iodixanol extender significantly improves the progressive motility, viability, plasma membrane integrity and acrosome integrity of cryopreserved semen from Thai native bulls, but it has no beneficial effect on in vitro fertilization ability and pregnancy rate after AI.
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Phirawit
authors.initials: P
authors.lastname: Chuawongboon
authors.affiliation: Department of Surgery and Theriogenology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Saksiri
authors.initials: S
authors.lastname: Sirisathien
authors.affiliation: Department of Livestock Development, Bureau of Biotechnology in Livestock Production, Bangkok, Thailand.
authors.forename: Jatuporn
authors.initials: J
authors.lastname: Pongpeng
authors.affiliation: Department of Livestock Development, Veterinary Research and Development Center (Upper Northeastern Region), Khon Kaen, Thailand.
authors.forename: Denpong
authors.initials: D
authors.lastname: Sakhong
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.|Food and Fertilizer Technology Center, Taipei, Taiwan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Thevin
authors.initials: T
authors.lastname: Vongpralub
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival; D015925:Cryopreservation; D005260:Female; D005306:Fertilization / Q000187:drug effects* / Q000502:physiology*; D007315:Insemination, Artificial; D008297:Male; D012661:Semen / Q000502:physiology*; D055101:Semen Analysis; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013785:Thailand; D014283:Triiodobenzoic Acids / Q000494:pharmacology*
chemical_list: D014283:Triiodobenzoic Acids; C044834:iodixanol
keywords: Thai indigenous cattle; cryopreservation; iodixanol; spermatozoa