title: Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution.
abstract: We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.
authors.affiliation: Kuban State Medical University, Krasnodar, Russia. elenakuevda@yandex.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Kuevda
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Gubareva
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Gumenyuk
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Sotnichenko
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Gilevich
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: R Z
authors.initials: RZ
authors.lastname: Nakokhov
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Rusinova
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: T G
authors.initials: TG
authors.lastname: Yudina
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Red'ko
authors.affiliation: Kuban State Medical University, Krasnodar, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Alekseenko
mesh_terms: D000818:Animals; D003217:Conductometry; D003840:Deoxycholic Acid / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D004553:Electric Conductivity; D008168:Lung / Q000166:cytology; D008297:Male; D017208:Rats, Wistar; D012996:Solutions; D023822:Tissue Engineering; D016707:Tissue Fixation; D054457:Tissue Scaffolds
chemical_list: D003902:Detergents; D012996:Solutions; D003840:Deoxycholic Acid
keywords: biological carcasses; conductometry; lung decellularization; rats; sodium deoxycholate