title: Use of response surface method for maximizing the production of arginine deiminase byPseudomonas putida.
abstract: Statistically designed experiments were used to optimize the production of arginine deiminase (ADI) byPseudomonas putidaKT2440 in batch culture. A Plackett-Burman design involving eleven factors showed that ADI production was most influenced by the initial pH and the initial concentrations of glucose and yeast extract. A central composite experimental design showed that the optimal values of these factors were 8.0, 10 g/L and 12.5 g/L, respectively. The other components of the optimal culture medium were bacto peptone 7.5 g/L, Triton X-100 0.30% (v/v), and arginine 3 g/L, for a culture temperature of 25 °C. Compared with the basal medium, the ADI activity in the optimized medium had nearly 4.5-fold increase (4.31 U/mL). The optimized medium was then used for a further study of ADI production in a 14 L stirred tank bioreactor. The agitation speed and the aeration rates were varied to determine suitable values of these variables.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
authors.forename: Mahesh D
authors.initials: MD
authors.lastname: Patil
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
authors.forename: Kiran D
authors.initials: KD
authors.lastname: Shinde
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Patel
authors.affiliation: School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Chisti
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
keywords: Arginine deiminase; Enzyme bioreactors; Enzyme production; Fermentation; Pseudomonas putida KT2440; Submerged culture