title: Comparative Transcriptomic Analysis of Vernalization- and Cytokinin-Induced Floral Transition in Dendrobium nobile.
abstract: Vernalization is required for floral initiation in Dendrobium. Interestingly, those beneficial effects can also be achieved by exogenous cytokinin application in greenhouses. Thus, an as yet unknown crosstalk/interaction may exist between vernalization and cytokinin signaling pathways. In this study, we showed, by de novo transcriptome assembly using RNA-seq data from both vegetative and reproductive tissue samples, that some floral transition-related genes-DnVRN1, FT, SOC1, LFY and AP1-were differentially expressed in low-temperature-challenged (LT) or thidiazuron (TDZ)-treated plants, compared to those mock-treated (CK). Both LT and TDZ upregulated SOC1, LFY and AP1, while the upregulation of DnVRN1 and FT was only LT-induced. We further found that LT promoted the upregulation of some key cytokinin signaling regulators, including several cytokinin biosynthesis-related genes and type-B response regulator (RR)-encoding genes, and that both LT and TDZ triggered the significant upregulation of some marker genes in the gibberellin (GA) signaling pathway, indicating an important low temperature-cytokinin-GA axis in flowering. Our data thus have revealed a cytokinin-GA signal network underlying vernalization, providing a novel insight into further investigation of the molecular mechanism of floral initiation in Dendrobium.
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Engineering Polytechnic, Guangzhou, 510520, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wen
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Guo
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Jinchi
authors.initials: J
authors.lastname: Li
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: He
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Yunquan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.|State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Qunyu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of life sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D003080:Cold Temperature; D016207:Cytokines / Q000378:metabolism*; D031667:Dendrobium / Q000187:drug effects / Q000254:growth & development*; D035264:Flowers / Q000187:drug effects / Q000254:growth & development*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism; D059467:Transcriptome
chemical_list: D016207:Cytokines; D010940:Plant Proteins