title: The intermembrane space protein Erv1 of Trypanosoma brucei is essential for mitochondrial Fe-S cluster assembly and operates alone.
abstract: Sulfhydryl oxidase Erv1 is a ubiquitous and conserved protein of the mitochondrial intermembrane space that plays a role in the transport of small sulfur-containing proteins. In higher eukaryotes, Erv1 interacts with the mitochondrial import protein Mia40. However, Trypanosoma brucei lacks an obvious Mia40 homologue in its genome. Here we show by tandem affinity purification and mass spectrometry that in this excavate protist, Erv1 functions without a Mia40 homologue and most likely any other interaction partner. Down-regulation of TbErv1 caused a reduction of the mitochondrial membrane potential already within 24h to less than 50% when compared with control cells. The depletion of TbErv1 was accompanied by accumulation of trCOIV precursor, with a concomitant reduction of aconitase activity both in the cytosol and mitochondrion. Overall, TbErv1 seems to have a role in the mitochondrial translocation and Fe-S cluster assembly in the organelle.
authors.affiliation: Institute of Parasitology, Biology Centre, 37005 České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic.
authors.forename: Alexander C
authors.initials: AC
authors.lastname: Haindrich
authors.affiliation: Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic.
authors.forename: Michala
authors.initials: M
authors.lastname: Boudová
authors.affiliation: Institute of Parasitology, Biology Centre, 37005 České Budějovice (Budweis), Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Vancová
authors.affiliation: Institute of Parasitology, Biology Centre, 37005 České Budějovice (Budweis), Czech Republic.
authors.forename: Priscila Peña
authors.initials: PP
authors.lastname: Diaz
authors.affiliation: Institute of Parasitology, Biology Centre, 37005 České Budějovice (Budweis), Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Horáková
authors.affiliation: Institute of Parasitology, Biology Centre, 37005 České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic; Canadian Institute for Advanced Research, Toronto, Ontario M5G 1Z8, Canada. Electronic address: jula@paru.cas.cz.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
mesh_terms: D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D013058:Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015800:Protozoan Proteins / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; D015800:Protozoan Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors
keywords: Erv1; Fe-S cluster assembly; Mitochondrion; Trypanosoma