title: Involved and Uninvolved Psoriatic Keratinocytes Display a Resistance to Apoptosis that may Contribute to Epidermal Thickness.
abstract: Psoriasis is a common autoimmune skin disease. The aim of this study was to investigate whether the apoptotic process is disturbed in psoriatic keratinocytes. In vitro culture of keratinocytes derived from both involved and uninvolved psoriatic skin, revealed higher viability and resistance to apoptosis following exposure to ultraviolet B, compared with cells from healthy controls. The position of apoptotic dysregulation was found to be upstream of cytochrome c release in the mitochondrial apoptotic pathway. Microarray transcriptome analysis revealed that 87 genes were differentially expressed in both involved and uninvolved psoriatic keratinocytes compared with controls. Among these, a general upregulation of anti-apoptotic genes and downregulation of pro-apoptotic genes were identified. This distinct apoptosis-resistant phenotype, unrelated to the inflammatory component of the disease, implies that intrinsic abnormalities in keratinocytes may contribute to the pathogenesis of psoriasis.
authors.affiliation: Ingrid Asp Psoriasis Research Center, Department of Clinical and Experimental Medicine, Faculty of Medicine and Health Sciences, Linköping University, SE-581 85 Linköping, Sweden.
authors.forename: Cecilia Bivik
authors.initials: CB
authors.lastname: Eding
authors.forename: Charlotta
authors.initials: C
authors.lastname: Enerbäck
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D011565:Psoriasis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011836:Radiation Tolerance; D014466:Ultraviolet Rays
chemical_list: D051017:Apoptosis Regulatory Proteins; D045304:Cytochromes c