title: Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders.
abstract: Peripheral blood mononuclear cells (PBMCs) have been used as a surrogate model of brain function in studies of psychiatric disorders, such as schizophrenia. This chapter describes the preparation of PBMCs from whole blood using a density gradient/cell culture protocol. This includes collection of the PBMC culture media and preparation of cell extracts in order to provide the raw materials for proteomic analyses.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK. hr288@cam.ac.uk.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002498:Centrifugation; D005362:Ficoll; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D001523:Mental Disorders / Q000097:blood* / Q000473:pathology; D040901:Proteomics / Q000379:methods*; D012559:Schizophrenia / Q000097:blood / Q000473:pathology; D013048:Specimen Handling / Q000379:methods
chemical_list: D001798:Blood Proteins; D005362:Ficoll
keywords: Cell model; Mass spectrometry; Multiplex immunoassay; PBMCs; Proteomics; Psychiatric disorders