title: Quantitative proteomic studies in resistance mechanisms of Eimeria tenella against polyether ionophores.
abstract: Polyether ionophores are widely used to treat and control coccidiosis in chickens. Widespread use of anticoccidials resulted in worldwide resistance. Mechanisms of resistance development and expansion are complex and poorly understood. Relative proteomic quantification using LC-MS/MS was used to compare sensitive reference strains (Ref-1, Ref-2) with putatively resistant and moderately sensitive field strains (FS-R, FS-mS) of Eimeria tenella after isotopic labelling with tandem mass tags (TMT). Ninety-seven proteins were identified, and 25 of them were regulated. Actin was significantly upregulated in resistant strains in comparison with their sensitive counterparts. On the other hand, microneme protein (MIC4) was downregulated in resistant strains. Optimization of labelling E. tenella sporozoites by TMT might identify further proteins that play a role in the obvious complex mechanism leading to resistance against Monensin.
authors.affiliation: Institute of Parasitology, Faculty of Veterinary Medicine, Centre for Infectious Diseases, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Thabet
authors.affiliation: Institute of Pharmacy, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany.
authors.forename: Walther
authors.initials: W
authors.lastname: Honscha
authors.affiliation: Institute of Parasitology, Faculty of Veterinary Medicine, Centre for Infectious Diseases, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany.|Albrecht-Daniel-Thaer-Institute, Leipzig, Germany.
authors.forename: Arwid
authors.initials: A
authors.lastname: Daugschies
authors.affiliation: Institute of Parasitology, Faculty of Veterinary Medicine, Centre for Infectious Diseases, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany. bangoura@vetmed.uni-leipzig.de.
authors.forename: Berit
authors.initials: B
authors.lastname: Bangoura
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000188:drug therapy* / Q000662:veterinary*; D003049:Coccidiostats / Q000494:pharmacology*; D004351:Drug Resistance; D016786:Eimeria tenella / Q000187:drug effects*; D007476:Ionophores / Q000494:pharmacology*; D008985:Monensin / Q000494:pharmacology; D011201:Poultry Diseases / Q000188:drug therapy*; D040901:Proteomics; D015800:Protozoan Proteins / Q000096:biosynthesis; D034101:Sporozoites / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000199:Actins; D003049:Coccidiostats; D007476:Ionophores; D015800:Protozoan Proteins; D008985:Monensin
keywords: Eimeria tenella; Polyether ionophores resistance; Proteomic; TMT