title: MiR-370 functions as oncogene in melanoma by direct targeting pyruvate dehydrogenase B.
abstract: BACKGROUND
  Accumulating evidence has shown that miR-370 play an important role in the development and progression of tumor. However, the role of miR-370 in melanoma remains largely unknown. The present study is designed to investigate the function of miR-370 in melanoma and to explore the molecular mechanism underlying its function.
  
  
  METHODS
  The expression level of miR-370 was detected in melanoma tissues and cell lines by real-time quantitative polymerase chain reaction (qRT-PCR). The effect of overexpression of miR-370 on in vitro cell proliferation, apoptosis, invasion as well as glyclolysis was examined. Western blotting analysis was used to detect the influence of miR-370 on the expression of target genes, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-370 in melanoma tissues.
  
  
  RESULTS
  Our study showed that miR-370 was upregulated in melanoma tissues compared with non-cancerous tissues (P<0.01). In addition, the expression of miR-370 in melanoma cell lines was also significantly higher (P<0.01). Enforced expression of miR-370 promotes melanoma cell proliferation, inhibits apoptosis and enhances invasion and glycolysis and led to downregulation of the PDHB protein. Moreover, the expression level of miR-370 in melanoma tissues showed inverse relationship with the expression level of PDHB protein.
  
  
  CONCLUSIONS
  In conclusion, our findings suggested that miR-370 represents a potential oncogenic miRNA and plays an important role in melanoma progression by directly targeting PDHB.
authors.affiliation: Department of Dermatology, Qilu Hospital of Shandong University, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Dermatology, Qilu Hospital of Shandong University, China. Electronic address: wymsdql@163.com.
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009857:Oncogenes / Q000235:genetics*; D010088:Oxidoreductases / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C526503:MIRN370 microRNA, human; D035683:MicroRNAs; D011773:Pyruvates; D010088:Oxidoreductases
keywords: Melanoma; MiR-370; PDHB