title: Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells: Biocompatibility and cryopreservation.
abstract: The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce "ready-to-use" tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany. Electronic address: mutsenko@imp.uni-hannover.de.
authors.forename: Vitalii V
authors.initials: VV
authors.lastname: Mutsenko
authors.affiliation: Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Gryshkov
authors.affiliation: Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Lauterboeck
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine.
authors.forename: Olena
authors.initials: O
authors.lastname: Rogulska
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine.
authors.forename: Dmitriy N
authors.initials: DN
authors.lastname: Tarusin
authors.affiliation: Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany.
authors.forename: Vasilii V
authors.initials: VV
authors.lastname: Bazhenov
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Schütz
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Sophie
authors.initials: S
authors.lastname: Brüggemeier
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Gossla
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Ashwini R
authors.initials: AR
authors.lastname: Akkineni
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Meißner
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Lode
authors.affiliation: BromMarin GmbH, Wernerstraße 1, 09599 Freiberg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Meschke
authors.affiliation: Department of Aquatic Zoology, Western Australian Museum, Locked Bag 49, Welshpool DC, Western Australia 6986, Australia.
authors.forename: Jane
authors.initials: J
authors.lastname: Fromont
authors.affiliation: Department of Biochemistry, Duke University School of Medicine, Durham, NC, USA.
authors.forename: Allison L
authors.initials: AL
authors.lastname: Stelling
authors.affiliation: International Institute of Biomineralization GmbH, Talweg 26, 09599 Freiberg, Germany.
authors.forename: Konstantin R
authors.initials: KR
authors.lastname: Tabachnik
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
authors.affiliation: Department of Aquatic Zoology, Western Australian Museum, Locked Bag 49, Welshpool DC, Western Australia 6986, Australia; Moscow Institute of Physics and Technology, 9 Institutskii per., 141700 Dolgoprudny, Moscow Region, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Nikulin
authors.affiliation: P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p.3, 125284 Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Rodin
authors.affiliation: P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p.3, 125284 Moscow, Russia.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Tonevitsky
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya str. 23, 61015 Kharkov, Ukraine.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Petrenko
authors.affiliation: Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Glasmacher
authors.affiliation: Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Carl-von-Ossietzky-Straße 9-11, 26111 Oldenburg, Germany.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schupp
authors.affiliation: Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany. Electronic address: Hermann.Ehrlich@physik.tu-freiberg.de.
authors.forename: Hermann
authors.initials: H
authors.lastname: Ehrlich
mesh_terms: D050156:Adipogenesis; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D002686:Chitin / Q000737:chemistry; D015925:Cryopreservation; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011161:Porifera / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D002686:Chitin
keywords: Chitin; Marine sponges; Mesenchymal stromal cells; Scaffolds; Tissue engineering