title: Cortical laminar tau deposits and activated astrocytes in Alzheimer's disease visualised by3H-THK5117 and3H-deprenyl autoradiography.
abstract: Hyperphosphorylated tau protein deposits and, inflammatory processes are characteristic components of Alzheimer disease (AD) pathology. We here aimed to visualize in vitro the distribution of tau deposits and activated astrocytes across the cortical layers in autopsy AD brain tissue using the radiotracers3H-THK5117 and3H-deprenyl.3H-THK5117 and3H-deprenyl autoradiographies were carried out on frozen brain sections from three AD patients and one healthy control.3H-THK5117 showed a distinct laminar cortical binding similar to3H-deprenyl autoradiography, with an extensive binding in the superficial and deep layers of the temporal neocortices, whereas the middle frontal gyrus showed an even binding throughout the layers. Globally, eventhough some differences could be observed, AT8 (tau) and GFAP (astrocyte) immunostaining showed a laminar pattern comparable to their corresponding radiotracers within each AD case. Some variability was observed between the AD cases reflecting differences in disease phenotype. The similar laminar cortical brain distribution of tau deposits and activated astrocytes supports the hypothesis of a close pathological interconnection. The difference in regional binding patterns of3H-THK5117 and AT8 antibody staining suggest additional tau binding sites detectable by3H-THK5117.
authors.affiliation: Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Translational Alzheimer Neurobiology, Karolinska Institutet, Stockholm S-14157, Sweden.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Lemoine
authors.affiliation: Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Translational Alzheimer Neurobiology, Karolinska Institutet, Stockholm S-14157, Sweden.
authors.forename: Laure
authors.initials: L
authors.lastname: Saint-Aubert
authors.affiliation: Department of Pathology, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Inger
authors.initials: I
authors.lastname: Nennesmo
authors.affiliation: Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Translational Alzheimer Neurobiology, Karolinska Institutet, Stockholm S-14157, Sweden.
authors.forename: Per-Göran
authors.initials: PG
authors.lastname: Gillberg
authors.affiliation: Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Translational Alzheimer Neurobiology, Karolinska Institutet, Stockholm S-14157, Sweden.|Department of Geriatrics, Karolinska University Hospital, Huddinge Stockholm, Sweden.
authors.forename: Agneta
authors.initials: A
authors.lastname: Nordberg
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001345:Autoradiography; D018728:Entorhinal Cortex / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D020534:Parahippocampal Gyrus / Q000378:metabolism / Q000473:pathology; D020005:Propanols / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D012642:Selegiline / Q000737:chemistry*; D014316:Tritium / Q000737:chemistry; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020005:Propanols; D011804:Quinolines; D016875:tau Proteins; D014316:Tritium; D012642:Selegiline