title: A two-photon NIR-to-NIR fluorescent probe for imaging hydrogen peroxide in living cells.
abstract: Hydrogen peroxide (H2O2), one of the reactive oxygen species (ROS), plays vital roles in diverse physiological processes. Imbalance of the H2O2is concerned with serious diseases such as cardiovascular disorders, neurodegenerative diseases, Alzheimer's disease and cancer. Therefore, it is critical to develop efficient methods for monitoring H2O2in vivo. In this work, a two-photon excitation (860nm) NIR fluorescent turn-on probe TPNR-H2O2for H2O2based on Dicyanomethylene-4H-pyran fluorophore is reported, which can be used in solution detection with 13.2-fold NIR fluorescence enhancement, fast response (completed within 40min), excellent sensitivity (DL 72.48nM), and lower cellular auto-fluorescence interference. Importantly, the perfect photostability of TPNR-H2O2clearly demonstrated that the probe could be applied to imaging intracellular H2O2for a long time without photobleaching. In addition, through two-photon imaging, this probe was cell permeable and used to monitor the level of endogenous and exogenous H2O2with promising biological application.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
authors.forename: Qichao
authors.initials: Q
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Du
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, PR China. Electronic address: pengxj@dlut.edu.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000302:isolation & purification*; D057054:Molecular Imaging / Q000379:methods*; D017785:Photons; D017382:Reactive Oxygen Species / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Cell imaging; Hydrogen peroxide; NIR emission; Two-photon; Ultra-low detection limit