title: Alternative splicing affects the targeting sequence of peroxisome proteins in Arabidopsis.
abstract: A systematic analysis of the Arabidopsis genome in combination with localization experiments indicates that alternative splicing affects the peroxisomal targeting sequence of at least 71 genes in Arabidopsis. Peroxisomes are ubiquitous eukaryotic cellular organelles that play a key role in diverse metabolic functions. All peroxisome proteins are encoded by nuclear genes and target to peroxisomes mainly through two types of targeting signals: peroxisomal targeting signal type 1 (PTS1) and PTS2. Alternative splicing (AS) is a process occurring in all eukaryotes by which a single pre-mRNA can generate multiple mRNA variants, often encoding proteins with functional differences. However, the effects of AS on the PTS1 or PTS2 and the targeting of the protein were rarely studied, especially in plants. Here, we systematically analyzed the genome of Arabidopsis, and found that the C-terminal targeting sequence PTS1 of 66 genes and the N-terminal targeting sequence PTS2 of 5 genes are affected by AS. Experimental determination of the targeting of selected protein isoforms further demonstrated that AS at both the 5' and 3' region of a gene can affect the inclusion of PTS2 and PTS1, respectively. This work underscores the importance of AS on the global regulation of peroxisome protein targeting.
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Chuanjing
authors.initials: C
authors.lastname: An
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Fuli
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. gaohongbo@bjfu.edu.cn.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Gao
mesh_terms: D017398:Alternative Splicing / Q000235:genetics / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D000074437:Peroxisomal Targeting Signal 2 Receptor / Q000235:genetics / Q000378:metabolism; D000074435:Peroxisome-Targeting Signal 1 Receptor / Q000235:genetics / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D021382:Protein Sorting Signals
keywords: Alternative splicing; Arabidopsis; PTS1; PTS2; Peroxisome; Subcellular localization