title: Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China.
abstract: Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, we describe an EMC virus isolate, identified as EMCV C15, which was successfully obtained from the serum of dogs from animal hospitals. Virus production in cell culture was confirmed by EMCV-specific real-time RT-PCR, indirect immunofluorescence assays and electron microscopy. In addition, the open reading frame sequence (ORF) of the EMCV C15 virus was determined. From sequence comparison and phylogenetic analysis among 24 reference EMCV strains, it appears that the EMCV C15 strain is closely genetically related to strain BEL2887A/91 (>99.0% nucleotide identity). In artificially challenged dogs, the heart and brain were important targets of EMCV C15. This study provides genetic and pathogenic characterization of the EMCV C15 strain isolated in Beijing and calls for sustained surveillance of EMCV infection in China to support better prevention and control of the disease.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.|Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, 100193, China.
authors.forename: Ya-Kun
authors.initials: YK
authors.lastname: Luo
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.|Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, 100193, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liang
authors.affiliation: Center for Nanyang Veterinary Biological Engineering Technology, Nanyang Normal University, Nanyang, 473061, China.
authors.forename: Qing-Hai
authors.initials: QH
authors.lastname: Tang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.|Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, 100193, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.|Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, 100193, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Shi
authors.affiliation: Department of Cell Biology, University Medical Center Groningen, 9713, AV, Groningen, The Netherlands.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Cong
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, 510642, China. linwencheng@cau.edu.cn.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Lin
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. cuishangjin@caas.cn.|Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, 100193, China. cuishangjin@caas.cn.
authors.forename: Shang-Jin
authors.initials: SJ
authors.lastname: Cui
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology; D018188:Cardiovirus Infections / Q000662:veterinary* / Q000821:virology; D002681:China; D016000:Cluster Analysis; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D004686:Encephalomyocarditis virus / Q000145:classification* / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect; D006321:Heart / Q000821:virology; D008854:Microscopy, Electron; D016366:Open Reading Frames; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D044967:Serum / Q000821:virology; D056189:Viral Tropism; D014776:Virus Cultivation