title: Inducible Inhibition of Gene Function with Photomorpholinos.
abstract: Photoactivatable morpholinos (MO) allow specific temporal and spatial inhibition of gene function, which is not possible with conventional morpholino or genetic global gene knock-out approaches. Here, we describe an application of commercially available photoactivatable MO technology for specific gene inhibition in a zebrafish embryonic model and discuss the required controls related to the specificity and efficacy of this method. A similar approach should be also applicable to other model organisms.
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave, Cincinnati, OH, 45230, USA. saulius.sumanas@cchmc.org.
authors.forename: Saulius
authors.initials: S
authors.lastname: Sumanas
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D017930:Genes, Reporter; D060172:Morpholinos / Q000737:chemistry / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000235:genetics; D010641:Phenotype; D014466:Ultraviolet Rays; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D060172:Morpholinos; D016376:Oligonucleotides, Antisense; D029961:Zebrafish Proteins
keywords: Morpholinos Caged; Photoactivatable; Zebrafish