title: Distribution and number of Cajal-like cells in testis tissue with azoospermia.
abstract: We investigated the number and distribution of Cajal-like cells in patients with azoospermia. A total of 99 patients with non-obstructive azoospermia were divided into subgroups [19 patientsin hypospermatogenesis group (S1), 40 patients in maturation arrest group (S2), 20 patients in a Sertoli cell-only syndrome (S3), and 20 patients in a testicular atrophy and fibrosis group (S4)], and 20 patients with obstructive azoospermia group (S0). Sections stained with a c-kit antibody were studied by light microscopy to determine the number and distribution of Cajal-like cells in peritubular and perivascular areas of testis. The number of Cajal-like cells were higher in all the non-obstructive groups than in the obstructive group (S0: 2.43 cells/mm2, S1: 3.14 cells/mm2, S2: 4.00 cells/mm2, S3: 4.57 cells/mm2, S4: 3.86 cells/mm2) but statistically significantly different (p<0.05) in the S2 and S3 subgroups only. Distribution of Cajal-like cells were similar in all groups. The number and distribution of Cajal-like cells in non-obstructive groups suggest that these cells may affect spermatogenesis. This cellular type can be responsible for the regulation of cellular motility or spermatogenesis. Electrophysiological and electron microscopic studies are needed to better define morphology and function of Cajal-like cells in the testis, especially totally the normal testis tissue.
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey. Electronic address: mdehasirci@gmail.com.
authors.forename: Eray
authors.initials: E
authors.lastname: Hasirci
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Turunc
authors.affiliation: Department of Pathology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Nebil
authors.initials: N
authors.lastname: Bal
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Mehmet Resit
authors.initials: MR
authors.lastname: Goren
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Celik
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Enis
authors.initials: E
authors.lastname: Kervancioglu
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Dirim
authors.affiliation: Department of Biostatistics, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Mustafa Agah
authors.initials: MA
authors.lastname: Tekindal
authors.affiliation: Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Ozkardes
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000473:pathology*; D002452:Cell Count; D006801:Humans; D056885:Interstitial Cells of Cajal / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000473:pathology; D013737:Testis / Q000473:pathology*
keywords: Male infertility; Non-obstructive azoospermia; Obstructive azoospermia; Testis; c-kit (+) cell