title: Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection.
abstract: Cytofluorimetric analysis is a typical method in immunology to evaluate phenotype and function of Natural Killer (NK) cells derived from HTLV-1/2 infected patients and healthy donors. Here, we described protocols to NK cells phenotypical and cytotoxicity assay, performed by flow cytometry on fresh and immune-magnetically or flow cytometry sorted NK cells. A new developed protocol able to evaluate IFNγ production has been included.
authors.affiliation: Molecular Immunology, University of Genova, Viale Benedetto XV 9, 16132, Genoa, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Bozzano
authors.affiliation: Molecular Immunology, University of Genova, Viale Benedetto XV 9, 16132, Genoa, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Marras
authors.affiliation: Molecular Immunology, University of Genova, Viale Benedetto XV 9, 16132, Genoa, Italy. de-maria@unige.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: De Maria
mesh_terms: D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D015490:HTLV-I Infections / Q000276:immunology* / Q000473:pathology; D015491:HTLV-II Infections / Q000276:immunology* / Q000473:pathology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology; D008297:Male
chemical_list: C573415:IFNG protein, human; D007371:Interferon-gamma
keywords: HTLV-1; HTLV-2; Inhibitory receptors; NK; NK cell functional analysis