title: Low level arsenite exposures suppress the development of bone marrow erythroid progenitors and result in anemia in adult male mice.
abstract: Epidemiological studies report an association between chronic arsenic (As) exposure and anemia in men, and women who are predisposed to anemia. The purpose of these studies was to determine whether a 60 d drinking water exposure of adult male C57BL/6J mice to 0, 100, and 500ppb arsenite (As+3) results in anemia due to alterations in erythroid progenitor cell development in the bone marrow. Exposure to 500ppb As+3for 60 d resulted in a reduction of mean corpuscular hemoglobin (MCH) levels, but did not significantly alter red blood cell (RBC) counts, hemoglobin (Hgb) levels, mean corpuscular Hgb concentrations (MCHC), or mean corpuscular volumes (MCV). Attenuation of burst-forming unit-erythroid (BFU-E) colony formation was observed in bone marrow cells of mice exposed to 500ppb As+3. The differentiation of late-stage bone marrow erythroblasts as defined by CD71 and Ter119 surface marker expression was reduced with the 500ppb As+3exposure. Mice exposed to 500ppb As+3also had elevated serum levels of erythropoietin (EPO). Collectively, these results show that exposure to low levels of As+3attenuate the development of early BFU-E cells and reduce the differentiation of late-stage erythroblasts. This suppression of bone marrow erythropoiesis may be a contributing factor to the mild hypochromic anemia observed in 500ppb As+3exposed mice.
authors.affiliation: The University of New Mexico College of Pharmacy, Department of Pharmaceutical Sciences, Albuquerque, NM 87131, United States.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Medina
authors.affiliation: The University of New Mexico College of Pharmacy, Department of Pharmaceutical Sciences, Albuquerque, NM 87131, United States.
authors.forename: Huan
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.
authors.forename: Shu Chun
authors.initials: SC
authors.lastname: Wang
authors.affiliation: The University of New Mexico College of Pharmacy, Department of Pharmaceutical Sciences, Albuquerque, NM 87131, United States.
authors.forename: Fredine T
authors.initials: FT
authors.lastname: Lauer
authors.affiliation: The University of New Mexico College of Pharmacy, Department of Pharmaceutical Sciences, Albuquerque, NM 87131, United States.
authors.forename: Ke Jian
authors.initials: KJ
authors.lastname: Liu
authors.affiliation: The University of New Mexico College of Pharmacy, Department of Pharmaceutical Sciences, Albuquerque, NM 87131, United States. Electronic address: sburchiel@salud.unm.edu.
authors.forename: Scott W
authors.initials: SW
authors.lastname: Burchiel
mesh_terms: D000740:Anemia / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004326:Drinking; D004785:Environmental Pollutants / Q000633:toxicity*; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000187:drug effects*; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000097:blood; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D008810:Mice, Inbred C57BL
chemical_list: D018053:Arsenites; D004785:Environmental Pollutants; D006454:Hemoglobins; D004921:Erythropoietin; C015001:arsenite
keywords: Anemia; Arsenite; Bone marrow; Erythroid progenitor cells; Erythropoiesis