title: Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data.
abstract: MicroRNAs have been reported to be involved in various biological processes. Here, we performed a systematic analysis to explore the clinical value and potential molecular mechanism of miR-145-5p in non-small cell lung cancer. First, a meta-analysis was performed with eligible literature, followed by microRNA microarrays in the Gene Expression Omnibus database, to verify the diagnostic and prognostic values of miR-145-5p. A cohort of 125 clinical paired non-small cell lung cancer samples was next used to detect the level of miR-145-5p and to explore the relationship of miR-145-5p with clinicopathological parameters. The Cancer Genome Atlas database was additionally applied to investigate the role of miR-145-5p in non-small cell lung cancer. The potential targets of miR-145-5p were predicted using 12 online prediction databases to explore the prospective molecular mechanism of miR-145-5p in non-small cell lung cancer. The expression of miR-145-5p in non-small cell lung cancer was significantly lower than that in healthy tissues. And miR-145-5p tended to show better diagnostic performance in lung squamous cell carcinoma than in lung adenocarcinoma. Furthermore, the expression of miR-145-5p was closely associated with lymph node metastasis in non-small cell lung cancer. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the target genes were mainly enriched with enzyme-linked receptor protein signaling pathways, SH3 domain binding, cell leading edge, and adherens junction. The protein-protein interaction network showed that eight hub genes (SMAD4, SMAD2, IRS1, FOXO1, ERBB4, NRAS, ACTB, and ACTG1) might be the key target genes of miR-145-5p in non-small cell lung cancer. The information we obtained might offer new perspectives for clinical diagnosis and treatment for non-small cell lung cancer.
authors.affiliation: 1 Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Ting-Qing
authors.initials: TQ
authors.lastname: Gan
authors.affiliation: 2 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Zu-Cheng
authors.initials: ZC
authors.lastname: Xie
authors.affiliation: 2 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Tang
authors.affiliation: 2 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Tong-Tong
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: 3 Department of Thoracic and Cardiovascular Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Dong-Yao
authors.initials: DY
authors.lastname: Li
authors.affiliation: 2 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Zu-Yun
authors.initials: ZY
authors.lastname: Li
authors.affiliation: 2 Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D005557:Formaldehyde; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D016612:Paraffin Embedding; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D016707:Tissue Fixation
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D005557:Formaldehyde
keywords: Gene Expression Omnibus database; MiR-145-5p; The Cancer Genome Atlas; bioinformatics; non-small cell lung cancer; target gene