title: Label-free protein quantification of sodium butyrate treated CHO cells by ESI-UHR-TOF-MS.
abstract: Effects of butyrate on CHO producer cells are contradictory, promoting productivity and at the same time repressing proliferation. Though in previous omics studies the background of butyrate impact on producer cells has been investigated, the knowledge about the mechanism is still very limited. As previous proteomic results on this field are mainly based on 2DE-gels, we conducted a label-free MS quantification, based on fast high resolution ESI-MS and a straight forward software solution, to gain insight in shifted cellular processes of CHO cells 25h after butyrate treatment. 118 proteins or subunits with significantly altered abundances were identified suggesting changes in carbohydrate, protein metabolic and cell cycle processes. Effects of butyrate on the nucleosome assembly as a known direct epigenetic influence on HDAC activity turned out to be unexpectedly fast and persistent, as confirmed by Western blots of histone-H4 acetylation. Contradictory to increased cell specific productivity, most elements of protein metabolism exhibited decreased levels after butyrate treatment. In comparison to published results some overlap of our label free MS data could be observed but also apparently diverging findings, showing the need for complementary omics techniques for a holistic view on cellular processes such as response to butyrate.
authors.affiliation: Institute of Cell Culture Technology, Bielefeld University, Germany; Biofidus AG, Bielefeld, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Müller
authors.affiliation: Institute of Cell Culture Technology, Bielefeld University, Germany; Xell AG, Bielefeld, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Heinrich
authors.affiliation: Bruker Daltonik GmbH, Bremen, Germany; Beuth University of Applied Sciences, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Jabs
authors.affiliation: Bruker Daltonik GmbH, Bremen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kaspar-Schönefeld
authors.affiliation: Institute of Cell Culture Technology, Bielefeld University, Germany; Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.affiliation: Institute of Cell Culture Technology, Bielefeld University, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Rodrigues de Carvalho
authors.affiliation: Bioinformatics Resource Facility, Center for Biotechnology (CeBiTec), Bielefeld University, Germany.
authors.forename: S P
authors.initials: SP
authors.lastname: Albaum
authors.affiliation: Bruker Daltonik GmbH, Bremen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Baessmann
authors.affiliation: Institute of Cell Culture Technology, Bielefeld University, Germany; Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Noll
authors.affiliation: Institute of Cell Culture Technology, Bielefeld University, Germany; Center for Biotechnology, Bielefeld University, Bielefeld, Germany. Electronic address: raimund.hoffrogge@uni-bielefeld.de.
authors.forename: R
authors.initials: R
authors.lastname: Hoffrogge
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid / Q000494:pharmacology*; D016466:CHO Cells / Q000187:drug effects* / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D003412:Cricetulus; D042421:Histone Code / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D009707:Nucleosomes / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D002087:Butyrates; D006657:Histones; D009707:Nucleosomes; D011506:Proteins; D020148:Butyric Acid
keywords: Carbohydrate metabolism; Chinese hamster ovary; Histone modification; Increased productivity; Label-free MS protein quantification; Protein biosynthesis