title: Outcomes of immature oocytes collected from ovarian tissue for cryopreservation in adult and prepubertal patients.
abstract: The efficiency of oocyte in-vitro maturation (IVM) and vitrification procedures after ex-vivo collection from ovarian tissue were assessed according to patient age, number of retrieved oocytes and tissue transport conditions. The combined procedure was performed in 136 patients: 130 adults (mean 27.6 ± 5.6 years) and six prepubertal girls (mean 8.7 ± 2.3 years). A higher mean number of oocytes were collected in girls compared with adults (11.5 ± 8.0 versus 3.8 ± 4.2, respectively, P < 0.001) but the percentage of degenerated oocytes was significantly higher in girls (35.5% versus 17.1%, respectively, P < 0.001). IVM rates were significantly lower in prepubertal than postpubertal population (10.3% versus 28.1%, P = 0.002). In adults, a negative correlation was observed between number of retrieved oocytes and age (P = 0.002; r = -0.271); the correlation was positive between anti-Müllerian hormone (AMH) and number of collected oocytes (P = 0.002; r = 0.264). IVM rates were not correlated with AMH levels (r = 0.06) or age (r = -0.033). At present, nine oocytes and one embryo have been warmed in four patients and one biochemical pregnancy obtained. This suggests the combined procedure could be an additional option for fertility preservation.
authors.affiliation: Research Laboratory on Human Reproduction, Campus Erasme, Université Libre de Bruxelles (ULB), Belgium; Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium. Electronic address: Giovanna.Fasano@erasme.ulb.ac.be.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Fasano
authors.affiliation: Research Laboratory on Human Reproduction, Campus Erasme, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Julie
authors.initials: J
authors.lastname: Dechène
authors.affiliation: Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Antonacci
authors.affiliation: Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Jamila
authors.initials: J
authors.lastname: Biramane
authors.affiliation: Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Vannin
authors.affiliation: Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Van Langendonckt
authors.affiliation: Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Devreker
authors.affiliation: Research Laboratory on Human Reproduction, Campus Erasme, Université Libre de Bruxelles (ULB), Belgium; Fertility Clinic, Department of Obstetrics and Gynecology, CUB-Erasme Hospital, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Demeestere
mesh_terms: D000328:Adult; D000367:Age Factors; D002648:Child; D015925:Cryopreservation; D005260:Female; D059247:Fertility Preservation / Q000706:statistics & numerical data*; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000706:statistics & numerical data*; D009865:Oocytes; D058989:Vitrification; D055815:Young Adult
keywords: Cryobanking; Fertility preservation; In-vitro maturation; Oocyte; Ovarian tissue; Vitrification