title: Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways.
abstract: Chronic renal failure (CRF) is a major public health problem worldwide. Hydrogen sulfide (H2S) plays important roles in renal physiological and pathophysiological processes. However, whether H2S could protect against CRF in rats remains unclear. In this study, we found that H2S alleviated gentamicin-induced nephrotoxicity by reducing reactive oxygen species (ROS)-mediated apoptosis in normal rat kidney-52E cells. We demonstrated that H2S significantly improved the kidney structure and function of CRF rats. We found that H2S decreased the protein levels of Bax, Caspase-3, and Cleaved-caspase-3, but increased the expression of Bcl-2. Treatment with H2S reduced the levels of malondialdehyde and ROS and increased the activities of superoxide dismutase and glutathione peroxidase. H2S significantly abolished the phosphorylation of extracellular signal-regulated protein kinase 1/2, c-Jun N-terminal kinase, and p38 in the kidney of CRF rats. Furthermore, H2S decreased the expression levels of tumor necrosis factor-α, interleukin (IL)-6, IL-10, and monocyte chemoattractant protein-1, as well as the protein levels of p50, p65, and p-p65 in the kidney of CRF rats. In conclusion, H2S could ameliorate adenine-induced CRF in rats by inhibiting apoptosis and inflammation through ROS/mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways.
authors.affiliation: Henan University School of Medicine, Kaifeng, 475004, Henan, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wu
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Lianqu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Luohe Medical College, Luohe, 462002, Henan, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Bu
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Yan
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Che
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Tengfu
authors.initials: T
authors.lastname: Zhao
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Henan University School of Medicine, Kaifeng, 475004, Henan, China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Ji
authors.affiliation: Henan University School of Medicine, Kaifeng, 475004, Henan, China. yanzhang206@163.com.
authors.forename: Yanzhang
authors.identifier: 0000-0001-6739-8437
authors.initials: Y
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Henan University, Kaifeng, 475001, Henan, China. Garricklee@foxmail.com.
authors.forename: Garrick D
authors.initials: GD
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007150:Immunohistochemistry; D007677:Kidney Function Tests; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051436:Renal Insufficiency, Chronic / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D016328:NF-kappa B; D020011:Protective Agents; D017382:Reactive Oxygen Species; D020928:Mitogen-Activated Protein Kinases; D006862:Hydrogen Sulfide