title: The possible molecular regulation mechanism of CIK cells inhibiting the proliferation of Human Lung Adenocarcinoma NCL-H157 Cells.
abstract: Cytokine-induced killer (CIK) cells were isolated and proliferation from human peripheral blood and cultured in appropriate growth medium. The biological characteristics of CIK cells were further determined by the characterization of surface markers by flow cytometry. CIK cells inhibited the proliferation of human lung adenocarcinoma NCL-H157 cells. Vascular endothelial growth factor (VEGF) expression was down-regulated in CIK cells co-cultured with NCL-H157 cells by western blotting analysis. Furthermore, in comparison with cells untreated by CIK, the NCL-H157 had a lower proliferation capacity. We proposed that the pharmacological mechanisms of NCL-H157 promoted by CIK can be estimated possibly with different biological significance that can be ascribed to down-regulated VEGF expression in vitro. The results suggest that the VEGF pathway guides developmental inhibiting of NCL-H157, and we speculate that the function of VEGF pathways is to guide NCL-H157 to inhibition by abundant CIK.
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, People's Republic of China.
authors.forename: Dengrui
authors.initials: D
authors.lastname: Li
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, People's Republic of China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of life science, Hebei normal university, Shijiazhuang, 050016, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, People's Republic of China.
authors.forename: Sumin
authors.initials: S
authors.lastname: Guo
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Hui
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, People's Republic of China.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Zhu
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Hou
authors.affiliation: The center of lung cancer prevention, Hebei province chest hospital, Shijiazhuang 050041, China , Tel:+86-311-86911298.
authors.forename: Shucai
authors.initials: S
authors.lastname: Wu
keywords: CIK; NCL-H157; VEGF; inhibition