title: Gene expression analysis of hypersensitivity to mosquito bite, chronic active EBV infection and NK/T-lymphoma/leukemia.
abstract: The human herpes virus, Epstein-Barr virus (EBV), is a known oncogenic virus and plays important roles in life-threatening T/NK-cell lymphoproliferative disorders (T/NK-cell LPD) such as hypersensitivity to mosquito bite (HMB), chronic active EBV infection (CAEBV), and NK/T-cell lymphoma/leukemia. During the clinical courses of HMB and CAEBV, patients frequently develop malignant lymphomas and the diseases passively progress sequentially. In the present study, gene expression of CD16(-)CD56(+)-, EBV(+)HMB, CAEBV, NK-lymphoma, and NK-leukemia cell lines, which were established from patients, was analyzed using oligonucleotide microarrays and compared to that of CD56brightCD16dim/-NK cells from healthy donors. Principal components analysis showed that CAEBV and NK-lymphoma cells were relatively closely located, indicating that they had similar expression profiles. Unsupervised hierarchal clustering analyses of microarray data and gene ontology analysis revealed specific gene clusters and identified several candidate genes responsible for disease that can be used to discriminate each category of NK-LPD and NK-cell lymphoma/leukemia.
authors.affiliation: a Department of Pediatrics , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.|b Department of Pathology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Washio
authors.affiliation: b Department of Pathology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Oka
authors.affiliation: b Department of Pathology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.|c Department of Pathology, Faculty of Medicine , Mansoura University , Egypt.
authors.forename: Lamia
authors.initials: L
authors.lastname: Abdalkader
authors.affiliation: a Department of Pediatrics , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Muraoka
authors.affiliation: a Department of Pediatrics , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shimada
authors.affiliation: a Department of Pediatrics , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Oda
authors.affiliation: b Department of Pathology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Hiaki
authors.initials: H
authors.lastname: Sato
authors.affiliation: b Department of Pathology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Takata
authors.affiliation: d Division of Molecular Medicine , Aichi Cancer Center Research Institute , Nagoya , Japan.
authors.forename: Yoshitoyo
authors.initials: Y
authors.lastname: Kagami
authors.affiliation: e Department of Virology, Division of Virology & Immunology , Medical Research Institute, Tokyo Medical and Dental University , Tokyo , Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: f Department of Pathology and Laboratory Medicine , Nagoya University Hospital , Nagoya , Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kato
authors.affiliation: g Department of Virology , Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.affiliation: h Department of Otorhinolaryngology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nishizaki
authors.affiliation: b Department of Pathology , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
authors.affiliation: a Department of Pediatrics , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsukahara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D002648:Child; D002908:Chronic Disease; D016000:Cluster Analysis; D009033:Culicidae / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D053263:Gene Regulatory Networks / Q000235:genetics; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology; D007299:Insect Bites and Stings / Q000276:immunology; D007694:Killer Cells, Natural / Q000378:metabolism / Q000473:pathology; D007938:Leukemia / Q000150:complications / Q000235:genetics* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000150:complications / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D015398:Signal Transduction / Q000235:genetics; D055815:Young Adult
keywords: CAEBV; HMB; NK-LPD; NK-lymphoma/leukemia; microarray