title: Isolation of a bacterial strain, Acinetobacter sp. from centrate wastewater and study of its cooperation with algae in nutrients removal.
abstract: Algae were able to grow healthy on bacteria-containing centrate wastewater in a pilot-scale bioreactor. The batch experiment indicated that the co-cultivation of algae and wastewater-borne bacteria improved the removal efficiencies of chemical oxygen demand and total phosphorus in centrate wastewater to 93.01% and 98.78%, respectively. A strain of beneficial aerobic bacteria, Acinetobacter sp., was isolated and its biochemical characteristics were explored. Synergistic cooperation was observed in the growth of algae and Acinetobacter sp. Removal efficiencies of some nutrients were improved significantly by the co-cultivation of algae and Acinetobacter sp. After treatment, residual nutrients in centrate wastewater reached the permissible discharge limit. The cooperation between algae and Acinetobacter sp. was in part attributed to the exchange of carbon dioxide and oxygen between the algae and bacteria. This synergetic relationship between algae and Acinetobacter sp. provided a promising way to treat the wastewater by improving the nutrients removal and biomass production.
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; Guangdong Provincial Engineering and Technology Research Center for Agricultural Land Pollution Prevention and Control, Guangzhou 510225, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN, USA.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Caibing
authors.initials: C
authors.lastname: Ming
authors.affiliation: Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Min
authors.affiliation: Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Chen
authors.affiliation: Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN, USA. Electronic address: ruanx001@umn.edu.
authors.forename: Roger
authors.initials: R
authors.lastname: Ruan
mesh_terms: D000150:Acinetobacter; D002708:Chlorella; D009584:Nitrogen / Q000302:isolation & purification; D010758:Phosphorus / Q000302:isolation & purification; D062065:Wastewater / Q000382:microbiology*
chemical_list: D009584:Nitrogen; D010758:Phosphorus; D062065:Wastewater
keywords: Acinetobacter sp.; Algae; Bacteria; Centrate wastewater; Nutrients removal