title: In Vitro Assessment of Anthelmintic Activities ofRauwolfia vomitoria(Apocynaceae) Stem Bark and Roots against Parasitic Stages ofSchistosoma mansoniand Cytotoxic Study.
abstract: Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots ofRauwolfia vomitoriaagainst two life stages ofSchistosoma mansoni. Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62.5-1000µg/mL and 250-1000µg/mL ofR. vomitoriastem bark and roots, respectively. The LC50values determined for the stem bark after 1 and 2 h of exposure were 207.4 and 61.18µg/mL, respectively. All adult worms exposed to the concentrations range of 250-1000µg/mL for both plant parts died within 120 h of incubation. The cytotoxic effects against HepG2 and Chang liver cell assessed using MTT assay method indicated that both plant extracts which were inhibitory to the proliferation of cell lines with IC50> 20μg/mL appear to be safe. This report provides the first evidence of in vitro schistosomicidal potency ofR. vomitoriawith the stem bark being moderately, but relatively, more active and selective against schistosome parasites. This suggests the presence of promising medicinal constituent(s).
authors.affiliation: Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, Cameroon; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: Emmanuel Mouafo
authors.identifier: 0000-0001-5167-0571
authors.initials: EM
authors.lastname: Tekwu
authors.affiliation: Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: Kwabena Mante
authors.initials: KM
authors.lastname: Bosompem
authors.affiliation: Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: William Kofi
authors.initials: WK
authors.lastname: Anyan
authors.affiliation: Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: Regina
authors.identifier: 0000-0003-4219-7107
authors.initials: R
authors.lastname: Appiah-Opong
authors.affiliation: Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: Kofi Baffour-Awuah
authors.initials: KB
authors.lastname: Owusu
authors.affiliation: Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: Mabel Deladem
authors.initials: MD
authors.lastname: Tettey
authors.affiliation: Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana.
authors.forename: Felicia Amanfo
authors.initials: FA
authors.lastname: Kissi
authors.affiliation: Centre for Plant Medicine Research (CPMR), Akwapim, Mampong, Ghana.
authors.forename: Alfred Ampomah
authors.initials: AA
authors.lastname: Appiah
authors.affiliation: Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, Cameroon.
authors.forename: Veronique
authors.initials: V
authors.lastname: Penlap Beng
authors.affiliation: School of Pharmacy, College of Health Sciences, University of Ghana, Accra, Ghana.
authors.forename: Alexander Kwadwo
authors.initials: AK
authors.lastname: Nyarko