title: Improvement of the Process Stability of Arylmalonate Decarboxylase by Immobilization for Biocatalytic Profen Synthesis.
abstract: The enzyme arylmalonate decarboxylase (AMDase) enables the selective synthesis of enantiopure (S)-arylpropinates in a simple single-step decarboxylation of dicarboxylic acid precursors. However, the poor enzyme stability with a half-life time of about 1.2 h under process conditions is a serious limitation of the productivity, which results in a need for high catalyst loads. By immobilization on an amino C2 acrylate carrier the operational stability of the (S)-selective AMDase variant G74C/M159L/C188G/V43I/A125P/V156L was increased to a half-life of about 8.6 days, which represents a 158-fold improvement. Further optimization was achieved by simple immobilization of the cell lysate to eliminate the cost- and time intensive enzyme purification step.
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Aßmann
authors.affiliation: Junior Research Group for Microbial Biotechnology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Mügge
authors.affiliation: Junior Research Group for Microbial Biotechnology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Sarah Katharina
authors.initials: SK
authors.lastname: Gaßmeyer
authors.affiliation: Junior Research Group for Microbial Biotechnology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Junichi
authors.initials: J
authors.lastname: Enoki
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Hilterhaus
authors.affiliation: Junior Research Group for Microbial Biotechnology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Kourist
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Liese
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany.
authors.forename: Selin
authors.initials: S
authors.lastname: Kara
keywords: arylmalonate decarboxylase; biocatalysis; enantioselectivity; immobilization; process stability; profen