title: On the Role of Additional [4Fe-4S] Clusters with a Free Coordination Site in Radical-SAM Enzymes.
abstract: The canonical CysXXXCysXXCys motif is the hallmark of the Radical-SAM superfamily. This motif is responsible for the ligation of a [4Fe-4S] cluster containing a free coordination site available for SAM binding. The five enzymes MoaA, TYW1, MiaB, RimO and LipA contain in addition a second [4Fe-4S] cluster itself bound to three other cysteines and thus also displaying a potentially free coordination site. This review article summarizes recent important achievements obtained on these five enzymes with the main focus to delineate the role of this additional [4Fe-4S] cluster in catalysis.
authors.affiliation: Biosciences and Biotechnology Institute of Grenoble, Laboratoire de Chimie et Biologie des Métaux, UMR 5249 CEA-Centre National de la Recherche Scientifique-UGA Grenoble, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Mulliez
authors.affiliation: Biosciences and Biotechnology Institute of Grenoble, Laboratoire de Chimie et Biologie des Métaux, UMR 5249 CEA-Centre National de la Recherche Scientifique-UGA Grenoble, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Duarte
authors.affiliation: Laboratoire de Chimie des Processus Biologiques, UMR 8229, Collége de France-Centre National de la Recherche Scientifique-Université P. et M. Curie Paris, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Arragain
authors.affiliation: Biosciences and Biotechnology Institute of Grenoble, Laboratoire de Chimie et Biologie des Métaux, UMR 5249 CEA-Centre National de la Recherche Scientifique-UGAGrenoble, France; Laboratoire de Chimie des Processus Biologiques, UMR 8229, Collége de France-Centre National de la Recherche Scientifique-Université P. et M. CurieParis, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Fontecave
authors.affiliation: Biosciences and Biotechnology Institute of Grenoble, Laboratoire de Chimie et Biologie des Métaux, UMR 5249 CEA-Centre National de la Recherche Scientifique-UGA Grenoble, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Atta
keywords: C-H bound activation; iron-sulfur clusters; macromolecule modifications; metalloenzymes; radical-SAM