title: Resveratrol protects podocytes against apoptosis via stimulation of autophagy in a mouse model of diabetic nephropathy.
abstract: Podocyte apoptosis coincides with albuminuria onset and precedes podocytopenia in diabetic nephropathy. However, there is a lack of effective therapeutic drugs to protect podocytes from apoptosis. Here, we demonstrated that resveratrol relieved a series of indicators of diabetic nephropathy and attenuated apoptosis of podocytes in db/db diabetic model mice. In addition, resveratrol induced autophagy in both db/db mice and human podocytes. Furthermore, inhibition of autophagy by 3-methyladenine (3-MA) and autophagy gene 5 (Atg5) short hairpin RNA (shRNA) reversed the protective effects of resveratrol on podocytes. Finally, we found that resveratrol might regulate autophagy and apoptosis in db/db mice and podocytes through the suppression of microRNA-383-5p (miR-383-5p). Together, our results indicate that resveratrol effectively attenuates high glucose-induced apoptosis via the activation of autophagy in db/db mice and podocytes, which involves miR-383-5p. Thus, this study reveals a new possible strategy to treat diabetic nephropathy.
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.|Department of Endocrinology, Nanjing General Hospital of Nanjing Military Command, Nanjing, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Huang
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Da-Fa
authors.initials: DF
authors.lastname: Ding
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Emergency, Yancheng First People's Hospital, Yancheng, China.
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Dong
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Ye
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Yang-Gang
authors.initials: YG
authors.lastname: Yuan
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Feng
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Na
authors.initials: N
authors.lastname: You
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Jia-Rong
authors.initials: JR
authors.lastname: Xu
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Miao
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: You
authors.affiliation: Department of Geriatics, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.
authors.forename: Yi-Bing
authors.initials: YB
authors.lastname: Lu
mesh_terms: D000419:Albuminuria / Q000188:drug therapy / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000235:genetics; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D050199:Podocytes / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D034741:RNA, Small Interfering; D013267:Stilbenes; D000077185:Resveratrol