title: Heterotrophic cultivation ofNannochloropsis salinafor enhancing biomass and lipid production.
abstract: Response surface methodology (RSM) was used to enhance the biomass and lipid content inNannochloropsissalinadue to its economic importance. Preliminary screening results revealed that the heterotrophically cultivatedN. salinawith various carbon and nitrogen sources yielded higher biomass (0.91 ± 0.0035 g/L) and lipid content (37.1 ± 0.49 mg/L) than that of the photoautotrophical cultivation (0.21 ± 0.009 g/L and 22.16 ± 0.27 mg/L). Significant sources that greatly influenced on biomass and lipid content of the alga were optimized through RSM. The medium consisting of glucose (7.959 g/L), sodium acetate (1.46 g/L), peptone (7.6 g/L) and sodium thiosulphate (1.05 g/L) was found to be the optimal concentration for heterotrophic cultivation by response optimizer. Confirmation experiment results for the RSM optimized concentration yielded the biomass of 1.85 g/L and total lipid content of 48.6 mg/L. In this study, we provide with a strategy for enhancing the biomass and lipid content inN. salina.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences Annamalai University, Parangipettai 608502, Tamil Nadu, India; Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Chennai 600119, India.
authors.forename: Thangapandi
authors.initials: T
authors.lastname: Marudhupandi
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences Annamalai University, Parangipettai 608502, Tamil Nadu, India.
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Sathishkumar
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences Annamalai University, Parangipettai 608502, Tamil Nadu, India; National Bureau of Fish Genetic Resources, Indian Council of Agriculture Research, Lucknow, Uttar Pradesh 226002, India.
authors.forename: Thipramalai Thankappan Ajith
authors.initials: TT
authors.lastname: Kumar
keywords: Biomass; Fatty acid; Heterotrophic cultivation; Nannochloropsis salina; Response surface methodology