title: Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure.
abstract: Scaffolds providing a 3D environment which can effectively promote the adhesion, proliferation and differentiation of cells are crucial to tissue regeneration. In this study, the poly-l-lactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase separation (TIPS). To mimic both physical architecture and chemical composite of natural bone extracellular matrix (ECM), gelatin fibers were introduced into the pores of PLLA scaffolds and formed 3D network structure via TIPS. Human adipose tissue-derived stem cells (ADSCs) were harvested and seeded into PLLA/gel hybrid scaffolds and culturedin vitrofor biocompatibility assay. The surface morphology, porosity and compressive modulus of scaffolds were characterized by scanning electron microscopy (SEM), density analysis and compression test respectively. The results showed that hybrid scaffolds had high porosity (91.62%), a good compressive modulus (2.79 ± 0.20 MPa), nanometer fibers (diameter around 186.39~354.30 nm) and different grades of pore size from 7.41 ± 2.64 nm to 387.94 ± 102.48 nm. The scaffolds with mild hydrolysis by NaOH were modified by 1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). Gelatin was performed onto PLLA scaffold via TIPS aiming at enhancement cell-material interaction. In comparison with PLLA scaffold, the PLLA/gel scaffold had better biological performance and the mechanical properties because the gelatin fibers homogeneously distributed in each pore of PLLA scaffold and formed 3D network structure.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. kedongsong@dlut.edu.cn.
authors.forename: Kedong
authors.initials: K
authors.lastname: Song
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. liliji@aliyun.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Ji
authors.affiliation: Medicine Department, Dalian University, Dalian 116622, China. zhangjingying@dlu.edu.cn.
authors.forename: Jingying
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. wanghai@dlut.edu.cn.
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. jiaozeren@mail.dlut.edu.cn.
authors.forename: Zeren
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 639798 Singapore, Singapore. mayasari.lim@gmail.com.
authors.forename: Lim
authors.initials: L
authors.lastname: Mayasari
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China. fuxiaoyan_111@aliyun.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. liutq@dlut.edu.cn.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Liu
keywords: bone tissue engineering; gelatin; human adipose derived stem cells; poly-l-lactic acid (PLLA); thermally induced phase separation