title: Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications.
abstract: Post-translational modifications (PTMs) are chemical alterations to individual amino acids that alter a protein's conformation, stability, and/or function. Several pathogenic viruses have been shown to encode proteins with PTMs, including human T-cell leukemia virus type 1 (HTLV-1) Tax and Rex regulatory proteins. HTLV-1 basic leucine zipper protein (HBZ) was hypothesized to feature PTMs due to its functional activities and interactions with cellular transcription factors and acetyltransferases. Here, we describe the approach used to identify, via mass spectrometry, the PTMs of HBZ. In addition, we describe methods to determine the functional relevance of the identified PTMs.
authors.affiliation: Center for Retrovirus Research, The Ohio State University, Columbus, OH, 43210, USA.|Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Al-Saleem
authors.affiliation: Center for Retrovirus Research, The Ohio State University, Columbus, OH, 43210, USA.|College of Pharmacy, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Mamuka
authors.initials: M
authors.lastname: Kvaratskhelia
authors.affiliation: Center for Retrovirus Research, The Ohio State University, Columbus, OH, 43210, USA. green.466@osu.edu.|Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, 43210, USA. green.466@osu.edu.|Compreshensive Cancer Center and Solove Research Institute, The Ohio State University, Columbus, OH, 43210, USA. green.466@osu.edu.|Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, 127N Veterinary Medicine Academic Building, 1900 Coffey Rd, Columbus, OH, 43210, USA. green.466@osu.edu.
authors.forename: Patrick L
authors.initials: PL
authors.lastname: Green
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016370:Gene Products, rex / Q000235:genetics / Q000378:metabolism; D016356:Gene Products, tax / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational; D012191:Retroviridae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D016370:Gene Products, rex; D016356:Gene Products, tax; C479751:HBZ protein, human T-cell leukemia virus type I; D012191:Retroviridae Proteins; C514844:rex Protein, Human T-lymphotropic virus 1; C514970:tax protein, Human T-lymphotrophic virus 1; D000217:Acyltransferases
keywords: HBZ; HTLV-1; IRF-1; Mass spectrometry; NF-κB; Post-translational modifications; c-Jun