title: Rapid detection of malachite green in fish based on CdTe quantum dots coated with molecularly imprinted silica.
abstract: A sensitive fluorescence sensor for the detection of malachite green (MG) was fabricated by grafting molecularly imprinted polymers (MIPs) onto the surface of CdTe quantum dots (QDs). The MIP-coated QDs were synthesized via a reverse microemulsion method using (3-aminopropyl)triethoxysilane (APTES) and tetraethyl orthosilicate (TEOS) as functional monomer and cross-linker, respectively. The optimum molar ratio of MG, functional monomer and cross-linker was 1:3:10. The MIP-coated QDs exhibited uniform spheres with diameter around 49nm and excellent fluorescence emission at λex370nm. A linear relationship with two segments between the relative fluorescence intensities and the MG concentrations ranging from 0.08 to 20μmol·L-1could be obtained with a detection limit of 12μg·kg-1. The fluorescent probe was successfully applied to the determination of MG in fish samples with the spiked recoveries ranging from 94.3% to 109.5% which were in accordance with those of the measurement by HPLC-UV.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Le
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Zheng-Zhong
authors.initials: ZZ
authors.lastname: Lin
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zhong
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Ai-Hong
authors.initials: AH
authors.lastname: Peng
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Chen
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361102, China. Electronic address: zhyhuang@jmu.edu.cn.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Huang
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry*; D005399:Fishes; D005506:Food Contamination; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D012394:Rosaniline Dyes; C005095:malachite green; D012822:Silicon Dioxide; D013691:Tellurium; C028337:cadmium telluride
keywords: CdTe quantum dots; Fish; Fluorescence probe; Malachite green; Molecularly imprinted polymers