title: In vitro the differences of inflammatory and oxidative reactions due to sulfur mustard induced acute pulmonary injury underlying intraperitoneal injection and intratracheal instillation in rats.
abstract: This study was to investigate the differences of inflammatory reaction and oxidative stress due to sulfur mustard (SM)-induced acute pulmonary injury via two ways in rats. In intraperitoneal and tracheal SM groups, injected intraperitoneally and instilled intratracheally with 0.1mL diluted SM (0.96 LD50=8mg/kg) and SM (0.98 LD50=2mg/kg) were administered in rats. In bronchoalveolar lavage fluid, serum, and alveolar septum, lactate dehydrogenase, glutathione peroxidase, tumor necrosis factor-α, interleukin-1β, interleukin-6, C-reactive protein, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, l-selectin, r-glutamyl transpeptidase, thiobarbituric acid reactive substances levels as well as the expression of CD4, CD20, CD68, 8-hydroxy deoxyguanosine, nuclear factor-E2-related factor 2, and heme oxygenase-1 measured by ELISA, immune scatter turbidimetry and immunohistochemical method in the intraperitoneal SM group were increased at each time-point compared with the tracheal SM groups, respectively. These data demonstrated an increased inflammatory reaction and oxidative stress indices in rat via intraperitoneal injection under similar SM LD50doses.
authors.affiliation: Department of Postgraduate, Weifang Medical College, Weifang 261053, China. Electronic address: ydwfmc@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Postgraduate, Weifang Medical College, Weifang 261053, China. Electronic address: yuanyuanbeiwfmc@163.com.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Bei
authors.affiliation: Department of Postgraduate, Weifang Medical College, Weifang 261053, China. Electronic address: liyuan6397@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiration, The 89th Hospital of PLA, Weifang 261021, China. Electronic address: weibaobaohao@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: State Key Laboratory of Antitoxic Drugs and Toxicology, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: yuxuzhong2008@163.com.
authors.forename: Yu-Xu
authors.initials: YX
authors.lastname: Zhong
authors.affiliation: Department of Respiration, The 89th Hospital of PLA, Weifang 261021, China. Electronic address: Cigamw@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Respiration, The 89th Hospital of PLA, Weifang 261021, China. Electronic address: yulingzhaoliu@sina.com.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: Department of Respiration, The 89th Hospital of PLA, Weifang 261021, China. Electronic address: xiaojizhu@163.com.
authors.forename: Xiao-Ji
authors.initials: XJ
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Antitoxic Drugs and Toxicology, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: jian_zhao@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002619:Chemical Warfare Agents; D002681:China; D016207:Cytokines / Q000378:metabolism; D003879:Dermatologic Agents / Q000008:administration & dosage*; D005979:Glutathione Peroxidase / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology*; D007274:Injections, Intraperitoneal; D007442:Intubation, Intratracheal; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007928:Lethal Dose 50; D008297:Male; D009151:Mustard Gas / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015815:Cell Adhesion Molecules; D002619:Chemical Warfare Agents; D016207:Cytokines; D003879:Dermatologic Agents; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; D009151:Mustard Gas
keywords: Acute pulmonary injury; Inflammatory reaction; Oxidative stress; Rat; Sulfur mustard