title: Effect of Supplemental Trace Minerals on Hsp-70 mRNA Expression in Commercial Broiler Chicken.
abstract: The effects of supplementing the organic forms of selenium (Se), chromium (Cr), and zinc (Zn) on Hsp-70 mRNA expression and body weight in broiler chickens were evaluated. 200 chicks were equally distributed into stainless steel battery brooders at the rate of 5 birds per pen and reared under heat stress condition up to 42ndday. The chicks were fed with three experimental diets supplemented with organic forms of Se (0.30 mg/kg), Cr (2 mg/kg), and Zn (40 mg/kg) during the starter and finisher phases and a control diet without any supplementation. On the 21st and 42nd day, 20 birds from each period were sacrificed and samples were collected for analysis. Organic Se, Cr, and Zn supplementation significantly (P < 0.05) reduced the expression of Hsp-70 mRNA levels. The Hsp-70 mRNA expression levels were significantly (P < 0.05) different between the tissues studied with spleen having the lowest expression level. Hsp-70 mRNA expression level was not affected by age of the birds. The study concluded that organic trace mineral (oTM) supplementation resulted in low Hsp-70 mRNA expression, indicating reduced heat stress in broilers.
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , Telangana , India.
authors.forename: U
authors.initials: U
authors.lastname: Rajkumar
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , Telangana , India.
authors.forename: A
authors.initials: A
authors.lastname: Vinoth
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , Telangana , India.
authors.forename: E Pradeep Kumar
authors.initials: EPK
authors.lastname: Reddy
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , Telangana , India.
authors.forename: M
authors.initials: M
authors.lastname: Shanmugam
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , Telangana , India.
authors.forename: S V Rama
authors.initials: SVR
authors.lastname: Rao
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000502:physiology; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response / Q000187:drug effects* / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution; D014131:Trace Elements / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D050884:HSP72 Heat-Shock Proteins; D012333:RNA, Messenger; D014131:Trace Elements
keywords: Chicken; Hsp-70; heat stress; trace minerals