title: Evaluation of cell interaction with polymeric biomaterials based on hyaluronic acid and chitosan.
abstract: Tissue engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for in vitro cell growth, and functional tissue development, that is subsequently implanted into patient. The aim of the current study was to evaluate the initial aspects of cell interaction with the polymeric biomaterials blends based on hyaluronic acid with chitosan. The hypothesis approach involves synthesis and analysis of swelling and thermal degradation (thermal gravimetric analysis) of the polymer blend; and Vero cell interaction with the biomaterial, through analysis of cytotoxicity, adhesion and cell morphology. The blend resulted in a biomaterial with a high swelling ratio that can allow nutrient distribution and absorption. The thermal gravimetric analysis results showed that the blend had two stages of degradation at temperatures very close to those observed for pure polymers, confirming that the physical mixing of hydrogels occurred, resulting in the presence of both hyaluronic acid and chitosan in the blend. The evaluation of indirect cytotoxicity showed that the blend was non cytotoxic for Vero cells, and the quantitative analysis performed with the MTT could verify a cell viability of 98%. The cells cultured on the blend showed adhesion, spreading and proliferation on this biomaterial, distinguished from the pattern of the control cells. These results showed that the blends produced from hyaluronic acid and chitosan hydrogels are promising for applications in tissue engineering, aiming at future cartilaginous tissue.
authors.affiliation: Human and Natural Sciences Center, ABC Federal University, Santo André, São Paulo, Brazil. monica.nasci@hotmail.com.
authors.forename: Mônica Helena Monteiro
authors.identifier: http://orcid.org/0000-0002-9721-996X
authors.initials: MHM
authors.lastname: do Nascimento
authors.affiliation: Human and Natural Sciences Center, ABC Federal University, Santo André, São Paulo, Brazil.
authors.forename: Mariselma
authors.initials: M
authors.lastname: Ferreira
authors.affiliation: Engineering, Modelling and Applied Social Sciences Center, ABC Federal University, Santo André, São Paulo, Brazil.
authors.forename: Sônia Maria
authors.initials: SM
authors.lastname: Malmonge
authors.affiliation: Engineering, Modelling and Applied Social Sciences Center, ABC Federal University, Santo André, São Paulo, Brazil.
authors.forename: Christiane Bertachini
authors.initials: CB
authors.lastname: Lombello
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D002522:Chlorocebus aethiops; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D008422:Materials Testing; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011108:Polymers; D006820:Hyaluronic Acid; D048271:Chitosan