title: The effect of prefreeze rejuvenation on postthaw storage of red blood cells in AS-3 and SAGM.
abstract: We investigated whether improving the metabolic status of red blood cell concentrates before freezing could extend the postthaw shelf life beyond 14 days while still meeting the requirements for hemolysis (0.8%) and total adenylate (>82% of original values).
  
  
  
  At Day 8 after collection, four leukoreduced red blood cell concentrates in saline-adenine-glucose-mannitol (SAGM) were pooled, mixed, and split (n = 4). Of these concentrates, two were rejuvenated in Rejuvesol. In addition, two leukoreduced red blood cell concentrates in phosphate-adenine-glucose-guanosine-gluconate-mannitol (PAGGGM) were pooled, mixed, and split at Day 8 after collection (n = 4). All concentrates were glycerolized, frozen, and stored for at least 2 weeks at -80°C. After thawing and deglycerolization, from each pair, one red blood cell concentrate was resuspended in SAGM, and one was suspended in AS-3. During postthaw storage at 2 to 6°C for 35 days, all concentrates were sampled weekly and analyzed for hematologic, metabolic, and morphologic parameters.
  
  
  
  Both Rejuvesol and PAGGGM treatment produced increased adenosine triphosphate and total adenylate and 2,3-diphosphoglycerate levels compared with untreated red blood cell concentrates. Regardless of prefreeze Rejuvesol or PAGGGM treatment, postthaw hemolysis remained below 0.8% during 7 days in SAGM and during 35 days in AS-3. At Day 35 of postthaw storage in AS-3, total adenylate in nonrejuvenated red blood cell concentrates had decreased to 72% of the original values; whereas, in prefreeze Rejuvesol-treated and PAGGGM-treated concentrates, adenylate values were still were at 101% and 98%, respectively.
  
  
  
  Based on maximum allowable hemolysis of 0.8% and total adenylate content greater than 82% of the original value, thawed, prefreeze Rejuvesol-treated or PAGGGM-treated red blood cell concentrates can be stored for 35 days at 2 to 6ºC in AS-3.
authors.affiliation: Department of Blood Cell Research, Sanquin Research.|Landsteiner Laboratory, Academic Medical Center, University of Amsterdam.
authors.forename: Charles C M
authors.identifier: 0000-0002-4144-0972
authors.initials: CCM
authors.lastname: Lelkens
authors.affiliation: Landsteiner Laboratory, Academic Medical Center, University of Amsterdam.|Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, the Netherlands.
authors.forename: Johan W M
authors.initials: JWM
authors.lastname: Lagerberg
authors.affiliation: Landsteiner Laboratory, Academic Medical Center, University of Amsterdam.|Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, the Netherlands.
authors.forename: Dirk
authors.initials: D
authors.lastname: de Korte
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000378:metabolism; D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects*; D005615:Freezing; D005947:Glucose / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D008353:Mannitol / Q000494:pharmacology
chemical_list: D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine