title: Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210.
abstract: Oxidative stress induces endothelial cell apoptosis and promotes atherosclerosis development. MicroRNA-210 (miR-210) is linked with apoptosis in different cell types. This study aimed to investigate the role of miR-210 in human umbilical vein endothelial cells (HUVECs) under oxidative stress and to determine the underlying mechanism. HUVECs were treated with different concentrations of hydrogen peroxide (H2O2), and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ATP assay. To evaluate the role of miR-210 in H2O2-mediated apoptosis, gain-and-loss-of-function approaches were used, and the effects on apoptosis and reactive oxygen species (ROS) level were assayed using flow cytometry. Moreover, miR-210 expression was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and expression of the following apoptosis-related genes was assessed by qRT-PCR and Western blot at the RNA and protein level, respectively: caspase-8-associated protein 2 (CASP8AP2), caspase-8, and caspase-3. The results showed that H2O2induced apoptosis in HUVECs in a dose-dependent manner and increased miR-210 expression. Overexpression of miR-210 inhibited apoptosis and reduced ROS level in HUVECs treated with H2O2. Furthermore, miR-210 downregulated CASP8AP2 and related downstream caspases at protein level. Thus, under oxidative stress, miR-210 has a prosurvival and antiapoptotic effect on HUVECs by reducing ROS generation and downregulating the CASP8AP2 pathway.
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Xianjing
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Jichang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Zhibo
authors.identifier: 0000-0002-4237-8613
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Youyou
authors.identifier: 0000-0002-5582-2373
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Xin
authors.identifier: 0000-0002-9414-005X
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Yinuo
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Bin
authors.identifier: 0000-0003-2017-2160
authors.initials: B
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D035683:MicroRNAs / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C485604:CASP8AP2 protein, human; D002135:Calcium-Binding Proteins; D035683:MicroRNAs; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D053148:Caspase 3; D053181:Caspase 8