title: Association of the c.-9C>T and c.368A>G transitions in H2BFWT gene with male infertility in an Iranian population.
abstract: The H2BFWT (H2B family, member W, testis specific) gene is a testis-specific histone which is involved in the spermatogenesis process. This study aimed to investigate the association of H2BFWT gene c.-9C>T and c.368A>G polymorphisms with male infertility in an Iranian population. The 232 independent individuals, including 109 infertile men and 123 healthy controls, were recruited from IVF centre (Kashan, Iran). The allele types of c.-9C>T and c.368A>G polymorphisms were detected by using PCR-RFLP method. In overall analysis, we found that the c.-9T (OR: 1.75, 95% CI: 1.04-2.95, p = .035) and c.368G (OR: 1.71, 95% CI: 1.02-2.89, p = .042) alleles are associated with male infertility. The c.-9T allele was also associated with nonobstructive azoospermia (OR: 2.08, 95% CI: 1.01-4.25, p = .046), while c.368G allele was associated with oligozoospermia (OR: 2.10, 95% CI: 1.15-3.85, p = .016). It is concluded that H2BFWT gene c.-9C>T and c.368A>G polymorphisms might be genetic risk factors for idiopathic male infertility.
authors.affiliation: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Rafatmanesh
authors.affiliation: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.|Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: H
authors.identifier: http://orcid.org/0000-0002-1454-7977
authors.initials: H
authors.lastname: Nikzad
authors.affiliation: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Ebrahimi
authors.affiliation: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Karimian
authors.affiliation: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: T
authors.initials: T
authors.lastname: Zamani
mesh_terms: D000328:Adult; D000483:Alleles; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006657:Histones / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D007492:Iran; D008297:Male; D020641:Polymorphism, Single Nucleotide; D013091:Spermatogenesis / Q000235:genetics
chemical_list: D006657:Histones
keywords: H2BFWT gene; PCR-RFLP; genetic polymorphism; male infertility