title: Analysis of Chromatin Dynamics During Drosophila Spermatogenesis.
abstract: In the course of spermatogenesis, germ cells undergo dramatic morphological changes that affect almost all cellular components. Therefore, it is impossible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating Drosophila male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat Drosophila sperm before immunofluorescence staining to help reveal epitopes in the highly condensed sperm chromatin that otherwise may be inaccessible to antibodies.
authors.affiliation: Department of Biology, Philipps-University Marburg, Karl-von-Frisch-Straße 8, 35043, Marburg, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Hundertmark
authors.affiliation: Department of Biology, Philipps-University Marburg, Karl-von-Frisch-Straße 8, 35043, Marburg, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Theofel
authors.affiliation: Department of Biology, Philipps-University Marburg, Karl-von-Frisch-Straße 8, 35043, Marburg, Germany.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Eren-Ghiani
authors.affiliation: Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, LS2 9JT, West Yorkshire, UK.
authors.forename: David
authors.initials: D
authors.lastname: Miller
authors.affiliation: Department of Biology, Philipps-University Marburg, Karl-von-Frisch-Straße 8, 35043, Marburg, Germany. rathke@biologie.uni-marburg.de.
authors.forename: Christina
authors.initials: C
authors.lastname: Rathke
mesh_terms: D000818:Animals; D002843:Chromatin / Q000276:immunology / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique / Q000295:instrumentation / Q000379:methods; D008297:Male; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology
chemical_list: D002843:Chromatin; D000939:Epitopes
keywords: Chromatin; Drosophila; Immunofluorescence; Sperm decondensation; Spermatogenesis