title: Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning.
abstract: Multiplexed photoluminescence (PL) detection plays an important role in chemical and biological sensing. Here, it is shown that time-gated (TG) detection of a single terbium-donor-based Förster resonance energy transfer (FRET) pair can be used to selectively quantify low nanomolar concentrations of multiple DNAs or microRNAs in a single sample. This study demonstrates the applicability of single-TG-FRET-pair multiplexing for molecular (Tb-to-dye) and nanoparticle (Tb-to-quantum-dot) biosensing. Both systems use acceptor-sensitization and donor-quenching for quantifying biomolecular recognition and modification of the donor-acceptor distance for tuning the PL decays. TG intensity detection provides extremely low background noise and a quick and simple one-step assay format. Single-TG-FRET-pair multiplexing can be combined with spectral and spatial resolution, paving the way for biosensing with unprecedented high-order multiplexing capabilities.
authors.affiliation: NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, 91405, Orsay Cedex, France.
authors.forename: Xue
authors.initials: X
authors.lastname: Qiu
authors.affiliation: NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, 91405, Orsay Cedex, France.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Guo
authors.affiliation: CAS/CUHK Research Centre for Biosensing and Medical Instrumentation, SIAT/CAS, Shenzhen, 518055, P. R. China.
authors.forename: Zongwen
authors.initials: Z
authors.lastname: Jin
authors.affiliation: NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, 91405, Orsay Cedex, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Petreto
authors.affiliation: Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Code 6900, Washington, DC, 20375, USA.
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
authors.affiliation: NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, 91405, Orsay Cedex, France.
authors.forename: Niko
authors.initials: N
authors.lastname: Hildebrandt
keywords: DNA; diagnostics; microRNA; optical barcoding; quantum dots