title: Comparison of two DNA extraction protocols from leave samples of Cotinus coggygria, Citrus sinensis and Genus juglans.
abstract: High quality DNA is essential for molecular research. Secondary metabolites can affect the quantity and quality DNA. In current research two DNA isolation methods including CTAB and Delaporta (protocols 1 &amp; 2 respectively) were applied in three leave samples from Cotinus coggygria, Citrus sinensis and Genus juglans that their leaves are rich of secondary metabolites. We successfully isolated DNA from C. coggygria, C. sinensis and Genus Juglans using the two protocols described above. Good quality DNA was isolated from C. coggygria, C. sinensis and Genus Juglans using protocol 1, while protocol 2 failed to produce usable DNA from these sources. The highest amount of DNA (1.3-1.6) was obtained from them using protocol 1. As we discovered, procedure 1 may work better for plants with secondary metabolites.
authors.affiliation: Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Fallah
authors.affiliation: Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Minaei Chenar
authors.affiliation: Department of Forestry, Faculty of Agriculture, Razi University, Kermanshah, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Amiri
authors.affiliation: Department of Forestry, Faculty of Agriculture, Razi University, Kermanshah, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Omodipour
authors.affiliation: Department of Forestry, Faculty of Agriculture, Razi University, Kermanshah, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Shirbande Ghods
authors.affiliation: Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran.
authors.forename: D
authors.initials: D
authors.lastname: Kahrizi
authors.affiliation: Zagros Bioidea Company, Razi University Incubator, Kermanshah, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Sohrabi
authors.affiliation: Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran.
authors.forename: T
authors.initials: T
authors.lastname: Ghorbani
authors.affiliation: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Kazemi
mesh_terms: D027926:Anacardiaceae / Q000235:genetics*; D032084:Citrus sinensis / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D031324:Juglans / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods; D018515:Plant Leaves / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D018744:DNA, Plant
keywords: Citrus sinensis; Cotinus coggygria; DNA extraction; Genus juglans.; secondary metabolites