title: Screening transesterifiable lipid accumulating bacteria from sewage sludge for biodiesel production.
abstract: Sewage sludge was evaluated as high available and low cost microbial oils feedstock for biodiesel production. Samples from four different wastewater treatment plants from La Araucanía Region in Southern Chile presented total lipids content ranging between 7.7 and 12.6%, being Vilcún sewage sludge that with the highest transesterifiable lipids content of about 50% of the total extracted lipids. The most relevant identified bacteria present in sludge samples wereAcinetobacter,PseudomonasandBacillus, beingBacillussp. V10 the strain with the highest transesterfiable lipids content of 7.4%.Bacillussp. V10 was cultured using urban wastewater supplemented with glucose to achieve nitrogen depleted medium and using milk processing wastewater as a low-cost carbon source.Bacillussp. V10 lipid profile indicates that low degree unsaturated long chain fatty acids such as C18:1 may account for approximately 50% of the lipids content, indicating its suitability to be used as raw material for biodiesel production.
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile; Department of Chemical Engineering, University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Mara
authors.initials: M
authors.lastname: Cea
authors.affiliation: Laboratory of Oils and Fats, Department of Agro-food Industry, Food and Nutrition, College of Agriculture "Luiz de Queiroz" (ESALQ), University of São Paulo, Piracicaba, São Paulo, Brazil.
authors.forename: Naiane
authors.initials: N
authors.lastname: Sangaletti-Gerhard
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Pedro
authors.initials: P
authors.lastname: Acuña
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Idi
authors.initials: I
authors.lastname: Fuentes
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile; Department of Chemical Sciences and Natural Resources, University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Milko
authors.initials: M
authors.lastname: Jorquera
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Karina
authors.initials: K
authors.lastname: Godoy
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Francisco
authors.initials: F
authors.lastname: Osses
authors.affiliation: Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile; Department of Chemical Engineering, University of La Frontera, P.O. Box 54-D, Temuco, Chile; Centre for Biotechnology & Bioengineering (CeBiB), University of La Frontera, P.O. Box 54-D, Temuco, Chile.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Navia
keywords: Activated sludge; Bacillus sp.; Biodiesel; Fatty acid methyl esters; Lipids