title: Y-box Binding Protein 1 Is Involved in Regulating the G2/M Phase of the Cell Cycle.
abstract: The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G1phase of the cell cycle by controlling transcription of G1regulators. Here, we report that YB1 is also involved in regulating G2/M phase.
  
  
  
  YB1-depleted TKO cells were subjected to quantitative reverse transcription-polymerase chain reaction and cell-cycle analysis. RNA immunoprecipitation (RIP)-chip assay was performed using anti-YB1 antibodies. Precipitated RNAs were subjected to microarray analysis.
  
  
  
  Silencing YB1 inhibited the proliferation of TKO cells, which lost the machinery required for G1phase arrest. Cell-cycle analysis showed that silencing YB1 caused G2/M phase cell-cycle arrest. RIP-chip assay showed that YB1 associated with mRNA of multiple cell-cycle-related genes, including G2/M phase regulators.
  
  
  
  YB1 positively regulates not only the G1phase but also G2/M phase by regulating multiple cell-cycle-related genes.
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan ykotake@fuk.kindai.ac.jp.|Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Kotake
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
authors.forename: Nagisa
authors.initials: N
authors.lastname: Arikawa
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Tahara
authors.affiliation: Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Maru
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Naemura
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D016195:G2 Phase / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D008938:Mitosis / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051840:Y-Box-Binding Protein 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human
keywords: Oncogene; YB1; cell cycle; p16; pRB