title: Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium.
abstract: Human somatic cells can be reprogrammed by defined factors to induced pluripotent stem cells (iPSCs). Importantly, the quality of iPSCs could impact the potential of these cells in basic and clinic research. Here, we describe a method to reprogram human fibroblast cells with Sendai virus in chemically defined conditions, to generate iPSCs that are integration-free and suitable for research and translational applications.
authors.affiliation: Faculty of Health Sciences, University of Macau, Taipa, Macau, China. GuokaiChen@umac.edu.|Center for Molecular Medicine, National Heart, Lung and Blood Institute, Bethesda, MD, USA. GuokaiChen@umac.edu.
authors.forename: Guokai
authors.initials: G
authors.lastname: Chen
authors.affiliation: New York Stem Cell Foundation Research Institute, New York, NY, USA. MRao@nyscf.org.|Q Therapeutics, Salt Lake City, UT, USA. MRao@nyscf.org.|Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston-Salem, NC, USA. MRao@nyscf.org.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Rao
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D029082:Sendai virus / Q000502:physiology
keywords: Chemically defined; Induced pluripotent stem cells; Reprogramming; Sendai virus