title: Data on optimization of expression and purification of AIMP2-DX2 protein inEscherichia coli.
abstract: AIMP2-DX2 is a splicing variant of AIMP2 protein which has been implicated in human lung cancer and chemoresistance of ovarian cancer (J.W. Choi, D.G. Kim, A.E. Lee, H.R. Kim, J.Y. Lee, N.H. Kwon, et al., 2011; J.W. Choi, J.W. Lee, J.K. Kim, H.K. Jeon, J.J. Choi, D.G. Kim, et al., 2012) [1,2]. We have shown, here, the data for the expression of AIMP2-DX2 protein inEscherichia coliand optimization of the critical steps in purification of AIMP2-DX2. The data described here has been successfully used to get a maximum yield of highly pure AIMP2-DX2 for subsequent characterization of its biophysical property in: "Purification and biophysical characterization of the AIMP2-DX2 protein" (R. Jha, H.Y. Cho, A. Ul Mushtaq, K. Lee, D.G. Kim, S. Kim, et al., 2017) [3].
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
authors.forename: Roshan
authors.initials: R
authors.lastname: Jha
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Cho
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
authors.forename: Ameeq Ul
authors.initials: AU
authors.lastname: Mushtaq
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.affiliation: Medicinal Bioconvergence Research Center, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Dae Gyu
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Medicinal Bioconvergence Research Center, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Jeon
keywords: AIMP2; AIMP2, Aminoacyl-tRNA synthetase interacting multifunctional protein 2; AIMP2-DX2; AIMP2-DX2 or DX2, splicing variant of AIMP2 lacking exon 2; DTT, dithiorietol; Expression and solubility; IPTG, isopropyl β-D-1-thiogalactopyranoside; Protein stabilizers; SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis; SUMO, Small Ubiquitin-like modifier; SUMO-tag