title: Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment.
abstract: The ATP-binding cassette (ABC) transporters belong to a superfamily of genes involved in the transport of specific molecules across lipid membranes, as well as insecticide resistance, present in all living organisms. In this study, we combined the Cnaphalocrocis medinals transcriptome database with a bioinformatics approach to identify four C. medinals ABCs (CmABCs), including CmABCG1, CmABCG4, CmABCC2 and CmABCC3. Tissue expression analysis showed that these genes had a tissue-specific expression pattern. CmABCG1 had significantly higher expression in the haemolymph and head compared to the other tissues. The expression of CmABCG4, CmABCC2 and CmABCC3 was highest in the midgut, followed by expression in the fat body. The developmental stage expression analysis showed that CmABCG1, CmABCG4, CmABCC2 and CmABCC3 were mainly expressed in adults. The transcription of CmABCG1, CmABCG4 and CmABCC2 was significantly induced by chlorpyrifos. Taken together, the results of our study provided useful information for understanding of the detoxification system of C. medinalis.
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Hai-Zhong
authors.initials: HZ
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Jia-Ping
authors.initials: JP
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Xue-Yang
authors.initials: XY
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Dong
authors.initials: D
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Dong-Qiong
authors.initials: DQ
authors.lastname: Fei
authors.affiliation: School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Rd., Heifei 230036, People's Republic of China (yuhaizhong1988@163.com; jiapingxu@ahau.edu.cn; wxy880528@sina.com; matafeiyan2016@163.com; m18856025885@163.com; fdqh1457@sina.com; 18755148780@163.com).
authors.forename: Shang-Zhi
authors.initials: SZ
authors.lastname: Zhang
authors.affiliation: Institute of Rice, Anhui Academy of Agricultural Sciences, 40 South Nongke Rd., Heifei 230031, People's Republic of China (wangwanlin0551@163.com).
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Wang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004390:Chlorpyrifos / Q000378:metabolism; D020869:Gene Expression Profiling; D008658:Inactivation, Metabolic; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000378:metabolism; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D018528:ATP-Binding Cassette Transporters; D007306:Insecticides; D004390:Chlorpyrifos
keywords: ABC transporter; Cnaphalocrocis medinalis; insecticide detoxification; mRNA expression