title: A novel combined polyphenol-aldehyde crosslinking of collagen film-Applications in biomedical materials.
abstract: Despite its crucial role in directing cell fate in healthy and diseased tissues, improvements in physical-chemical properties and biocompatibility of type-I collagen are still needed. In this report, we described combined and facile method to modify collagen. The collagen film was first modified by procyanidins solution, in which, then subjected to further crosslinked by dialdehyde alginate, resulting in collagen-procyanidins-dialdehyde alginate film. The properties of the crosslinked collagen films were investigated and the results were discussed. Results from differential scanning calorimetry and thermo gravimetric analysis suggested that the thermal stabilities of the collagen-procyanidins-dialdehyde alginate film were significantly improved. The mechanical properties of collagen-procyanidins-dialdehyde alginate film in terms of elongation at break and tensile strength increased approximately 2-fold and 3-fold, respectively compare to pure collagen film. In addition, the resistance to collagenase degradation of collagen-procyanidins-dialdehyde alginate film was remarkably promoted. The results from methyltetrazolium assay and confocal laser scanning microscopy showed that no cytotoxicity of collagen film was introduced by the combined crosslinking method. Thus, the novel combined by procyanidins-dialdehyde alginate crosslinking method shown in this study provided a non-toxic and efficient crosslinking method that improved various properties of collagen film, which has great potential applications in biomedical materials.
authors.affiliation: Research Center of Biomedical Engineering, Sichuan University, Chengdu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Research Center of Biomedical Engineering, Sichuan University, Chengdu, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Research Center of Biomedical Engineering, Sichuan University, Chengdu, China. Electronic address: danweihua_scu@126.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Dan
authors.affiliation: Research Center of Biomedical Engineering, Sichuan University, Chengdu, China.
authors.forename: Nianhua
authors.initials: N
authors.lastname: Dan
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D044946:Biflavonoids / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002392:Catechin / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000737:chemistry* / Q000633:toxicity; D003432:Cross-Linking Reagents / Q000737:chemistry*; D055595:Mechanical Phenomena; D059808:Polyphenols / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry
chemical_list: D000447:Aldehydes; D044946:Biflavonoids; D001672:Biocompatible Materials; D024042:Collagen Type I; D003432:Cross-Linking Reagents; D059808:Polyphenols; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin
keywords: Collagen film; Dialdehyde alginate; Procyanidins