title: Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status.
abstract: In mammals, transcription in the zygote begins after fertilization. This transcriptional wave is called zygotic gene activation (ZGA). During ZGA, epigenetic modifications, such as DNA methylation and histone modifications, are dynamically and drastically reconstructed in a sequence-specific manner. However, how such orchestrated gene upregulation is regulated remains unknown. Recently, using microinjection techniques, we have revealed that a class of long noncoding RNAs, named promoter-associated noncoding RNAs (pancRNAs), mediates specific gene upregulation through promoter DNA demethylation during ZGA. Here, we describe the experimental methods available to control the expression levels of pancRNAs and to evaluate epigenetic status after pancRNA manipulation.
authors.affiliation: Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hamazaki
authors.affiliation: Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Kinichi
authors.initials: K
authors.lastname: Nakashima
authors.affiliation: Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, 812-8582, Japan. imamura@scb.med.kyushu-u.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Imamura
mesh_terms: D000818:Animals; D019175:DNA Methylation; D047108:Embryonic Development / Q000235:genetics*; D044127:Epigenesis, Genetic; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D051379:Mice; D008845:Microinjections; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D022661:RNA, Untranslated / Q000008:administration & dosage / Q000235:genetics*
chemical_list: D022661:RNA, Untranslated
keywords: DNA methylation; Long noncoding RNA; Microinjection; Mouse