title: Iron chelators inhibit the heme-degradation reaction by HutZ from Vibrio cholerae.
abstract: HutZ is a heme-degrading enzyme. We found that the heme-degradation reaction by HutZ is inhibited by the iron chelators. Kinetic analysis of each heme-degradation step suggests that water molecules hydrogen bonded to Thr27 are involved in proton transfer to Fe(iii)-OO-, and that this step is inhibited by iron chelators.
authors.affiliation: Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-8628, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Dojun
authors.forename: Yukari
authors.initials: Y
authors.lastname: Sekine
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ishimori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uchida
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D005290:Ferric Compounds / Q000737:chemistry; D006418:Heme / Q000737:chemistry / Q000378:metabolism*; D006859:Hydrogen / Q000737:chemistry; D007501:Iron / Q000737:chemistry / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology*; D007700:Kinetics; D013912:Threonine / Q000737:chemistry; D014734:Vibrio cholerae / Q000201:enzymology*; D014867:Water / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D014867:Water; C000499:ferric oxide; D013912:Threonine; D006418:Heme; D006859:Hydrogen; D007501:Iron