title: Downregulation of LIMK1-ADF/cofilin by DADS inhibits the migration and invasion of colon cancer.
abstract: This study aimed to explore whether the downregulation of LIM kinase 1 (LIMK1)-actin depolymerization factor (ADF, also known as destrin)/cofilin by diallyl disulfide (DADS) inhibited the migration and invasion of colon cancer. Previous studies have shown that silencing LIMK1 could significantly enhance the inhibitory effect of DADS on colon cancer cell migration and invasion, suggesting that LIMK1 was a target molecule of DADS, which needed further confirmation. This study reported that LIMK1 and destrin were highly expressed in colon cancer and associated with poor prognosis of patients with colon cancer. Also, the expression of LIMK1 was positively correlated with the expression of destrin. The overexpression of LIMK1 significantly promoted colon cancer cell migration and invasion. DADS obviously inhibited migration and invasion by suppressing the phosphorylation of ADF/cofilin via downregulation of LIMK1 in colon cancer cells. Furthermore, DADS-induced suppression of cell proliferation was enhanced and antagonized by the knockdown and overexpression of LIMK1 in vitro and in vivo, respectively. Similar results were observed for DADS-induced changes in the expression of vimentin, CD34, Ki-67, and E-cadherin in xenografted tumors. These results indicated that LIMK1 was a potential target molecule for the inhibitory effect of DADS on colon cancer cell migration and invasion.
authors.affiliation: Department of Pathology, The Second Affiliated Hospital, University of South China, Hengyang, Hunan, China.|Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, Cancer Research Institute, University of South China, Hengyang, Hunan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Su
authors.affiliation: Key Laboratory of Translational Radiation Oncology, Hunan Province, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, University of South China, Hengyang, Hunan, China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, Cancer Research Institute, University of South China, Hengyang, Hunan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shi
authors.affiliation: Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, Cancer Research Institute, University of South China, Hengyang, Hunan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, University of South China, Hengyang, Hunan, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Liao
authors.affiliation: Key Laboratory of Translational Radiation Oncology, Hunan Province, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
authors.forename: Qianjin
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, Cancer Research Institute, University of South China, Hengyang, Hunan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
mesh_terms: D000498:Allyl Compounds / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051345:Destrin / Q000378:metabolism*; D004220:Disulfides / Q000008:administration & dosage*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D054461:Lim Kinases / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010766:Phosphorylation
chemical_list: D000498:Allyl Compounds; C495824:DSTN protein, human; D051345:Destrin; D004220:Disulfides; C028009:diallyl disulfide; C515892:LIMK1 protein, human; D054461:Lim Kinases