title: Association of angiotensinogen gene SNPs and haplotypes with risk of hypertension in eastern Indian population.
abstract: BACKGROUND
  Angiotensinogen (AGT) enzyme comprises a vital module of RAAS system that effectively controls the blood pressure and related cardiovascular functions. Ample association studies have reported the importance of AGT variants in cardiovascular and non-cardiovascular adversities. But lately, owing to the complexity of the many anomalies, the haplotype based examination of genetic variation that facilitates the identification of polymorphic sites which are located in the vicinity of the causative polymorphic site, gets greater appreciation.
  
  
  METHODS
  In the present study, we have done genotype and haplotype analysis of AGT gene in reference to hypertension to confirm the association of the two in an Indian population. To accomplish this, we performed candidate SNPs analysis and construct possible haplotypes across theAGTpromoter and gene region in 414 subjects (256 Hypertensive cases and 158 controls).
  
  
  RESULTS
  We found four SNPs (rs11568020: A-152G and rs5050: A-20C in promoter; rs4762 and rs699 in exon2) and 3 haplotypes (H4, H7 and H8) that showed a stronger positive association with hypertension. The haplotype H2 was showing protective association with hypertension.
  
  
  CONCLUSIONS
  The results of the present study confirmed and reestablished the role of AGT gene variants and their haplotypes in the causation of hypertension in Indian population and showed that haplotypes can provide stronger evidence of association.
authors.affiliation: DNA Laboratory, Anthropological Survey of India, Western Regional Center, Udaipur, 313001 Rajasthan India.|DNA Laboratory, Anthropological Survey of India, 27 Jawaharlal Nehru Road, Kolkata, 700016 India.|Department of Anthropology, Andhra University, Visakhapatnam, 530003 Andhra Pradesh India.
authors.forename: Pulakes
authors.initials: P
authors.lastname: Purkait
authors.affiliation: Department of Molecular Biology, BrahmanandaKeshab Chandra College, 111/2 B.T.Road, BonHooghly, P.O. - BonHooghly, Kolkata, 700 108 West Bengal India.
authors.forename: Kalpataru
authors.initials: K
authors.lastname: Halder
authors.affiliation: Department of Anthropology, University of Delhi, North Campus, Delhi, 110007 India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Thakur
authors.affiliation: DNA Laboratory, Anthropological Survey of India, 27 Jawaharlal Nehru Road, Kolkata, 700016 India.
authors.forename: Abhishikta
authors.initials: A
authors.lastname: Ghosh Roy
authors.affiliation: Department of Endocrinology, Calcutta Medical College & Hospital, 88, College Street, Kolkata, 700073 India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Raychaudhuri
authors.affiliation: Department of Nephrology & Dialysis, B.P. Poddar Hospital & Medical Research LTD, 71/1 HumayunKabirSarani, New Alipore, Block - G, Kolkata, 700053 West Bengal India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: DNA Laboratory, Anthropological Survey of India, 27 Jawaharlal Nehru Road, Kolkata, 700016 India.
authors.forename: B N
authors.initials: BN
authors.lastname: Sarkar
authors.affiliation: Department of Anthropology, Andhra University, Visakhapatnam, 530003 Andhra Pradesh India.
authors.forename: J M
authors.initials: JM
authors.lastname: Naidu
keywords: Angiotensinogen; Haplotype; Hypertension; Indian population; Methylation; SNP