title: Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China.
abstract: To investigate bat coronaviruses (CoVs), we collected 132 rectal swabs and urine samples from five bat species in three countries in southwestern China. Seven CoVs belonging to distinct groups of severe acute respiratory syndrome (SARS)-like CoVs and α-CoVs were detected in samples from least horseshoe bats. Samples from other bat species were negative for these viruses, indicating that the least horseshoe bat represents one of the natural reservoirs and mixers for strains of CoVs and has a pivotal role in the evolution and dissemination of these viruses. The genetic and evolutionary characteristics of these strains were described. Whole-genome sequencing of a new isolate (F46) from a rectal swab from a least horseshoe bat showed that it contained 29 699 nucleotides, excluding the poly (A) tail, with 13 open reading frames (ORFs). Phylogenetic and recombination analyses of F46 provided evidence of natural recombination between bat SARS-like CoVs (Rs3367 and LYRa11) or SARS-CoV (BJ01), suggesting that F46 could be a new recombinant virus from SARS-like CoVs or SARS-CoVs.
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
authors.forename: Shihong
authors.initials: S
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Liang
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000145:classification / Q000821:virology*; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D019143:Evolution, Molecular; D059646:Genome Size; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012007:Rectum / Q000821:virology; D014556:Urine / Q000821:virology
chemical_list: D012367:RNA, Viral