title: A fluorescent glycosyl-imprinted polymer for pH and temperature regulated sensing of target glycopeptide antibiotic.
abstract: This paper demonstrates a new strategy for developing a fluorescent glycosyl-imprinted polymer for pH and temperature regulated sensing of target glycopeptide antibiotic. The technique provides amino modified Mn-doped ZnS QDs as fluorescent supports, 4-vinylphenylbronic acid as a covalent monomer, N-isopropyl acrylamide as a thermo-responsive monomer in combination with acrylamide as a non-covalent monomer, and glycosyl moiety of a glycopeptide antibiotic as a template to produce fluorescent molecularly imprinted polymer (FMIP) in aqueous solution. The FMIP can alter its functional moieties and structure with pH and temperature stimulation. This allows recognition of target molecules through control of pH and temperature. The fluorescence intensity of the FMIP was enhanced gradually as the concentration of telavancin increased, and showed selective recognition toward the target glycopeptide antibiotic preferentially among other antibiotics. Using the FMIP as a sensing material, good linear correlations were obtained over the concentration range of 3.0-300.0μg/L and with a low limit of detection of 1.0μg/L. The analysis results of telavancin in real samples were consistent with that obtained by liquid chromatography tandem mass spectrometry.
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Kuncai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Luo
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
authors.forename: Pengzhe
authors.initials: P
authors.lastname: Qin
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China. Electronic address: jsutanlei@gmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.affiliation: MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510006, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China. Electronic address: yangzc@gzcdc.org.cn.
authors.forename: Zhicong
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D000077427:Lipoglycopeptides; D008345:Manganese; D011108:Polymers / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013696:Temperature; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D000077427:Lipoglycopeptides; D011108:Polymers; D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; C031238:zinc sulfide; C487637:telavancin
keywords: Fluorescence probes; Glycopeptide antibiotics; Molecular imprinting; Quantum dots