title: Spasmolytic and Antibacterial Activity of TwoCitrus sinensisOsbeck Varieties Cultivated in Mexico.
abstract: Antibacterial activity on ATCC strains ofEscherichia coli,Salmonella enterica,Salmonella enteritidis,andSalmonella choleraesuisand spasmolytic effect on contraction on rat ileum trips were determinate. Eight organic extracts (hexanic and methanolic) of albedo (mesocarp) and flavedo (pericarp) of two varieties (Valencian and National) ofCitrus sinensis(L.) Osbeck of Yucatán, México, were studied. Additionally, chromatographic fingerprints were obtained and correlated with their pharmacological effects. MAN, MAV, and HFN extract caused inhibition againstS. choleraesuis(MIC: 1000µg/mL) andS. enteritidis(MIC: 1000µg/mL). Regarding the spasmolytic effect, the Valencian extracts variety was more efficient on spontaneous contraction, HAV (Emax= 51.98 ± 1.98%), MAV (Emax= 35.98 ± 1.42%), HFV (Emax= 68.91 ± 4.14%), and MFV (Emax= 51.28 ± 2.59%), versus National variety, HAN (Emax= 43.80 ± 6.32%), MAN (Emax= 14.62 ± 1.69%), HFN (Emax= 64.87 ± 3.04%), and MFN (Emax= 31.01 ± 3.92%). Chromatographic fingerprints of HFV and HFN were found to have some similar signals that belong to monoterpenes, whereas for HAN and HAV similar signals were found belonging to fatty acids and triterpenoids. Methanolic extracts showed signals of (1) furfural, (2) furfural acetone (3) furfuraldehyde and (4)β-sitosterol compounds. Flavedo portion ofC. sinensispossessed spasmolytic effect on rat ileum strips and antibacterial activity againstSalmonellastrains. This species is source for obtaining bioactive compounds with therapeutic potential in the treatment of infectious diarrhea.
authors.affiliation: Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, YUC, Mexico.
authors.forename: Amanda
authors.initials: A
authors.lastname: Sánchez-Recillas
authors.affiliation: Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, YUC, Mexico.
authors.forename: Ana Ly
authors.initials: AL
authors.lastname: Arroyo-Herrera
authors.affiliation: Laboratorio de Cromatografía, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, YUC, Mexico.
authors.forename: Jesús Alfredo
authors.initials: JA
authors.lastname: Araujo-León
authors.affiliation: CONACYT, Departamento de Recursos del Mar, CINVESTAV-Unidad Mérida, Mérida, YUC, Mexico.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Hernández Núñez
authors.affiliation: Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, YUC, Mexico.
authors.forename: Rolffy
authors.identifier: 0000-0001-8822-7389
authors.initials: R
authors.lastname: Ortiz Andrade