title: Plant tissue cultures as sources of new ene- and ketoreductase activities.
abstract: While many redox enzymes are nowadays available for synthetic applications, the toolbox of ene-reductases is still limited. Consequently, the screening for these enzymes from diverse sources in the search of new biocatalyst suitable for green chemistry approaches is needed. Among 13 plant tissue cultures, Medicago sativa and Tessaria absinthioides calli, as well as Capsicum annuum hairy roots, were selected due to their ability to hydrogenate the CC double bond of the model substrate 2-cyclohexene-1-one. The three axenic plant cultures showed more preference toward highly activated molecules such as nitrostyrene and maleimide rather than the classical substrates of the well-known Old Yellow Enzymes, resembling the skills of the NAD(P)H-dependent flavin-independent enzymes. When the three biocatalytic systems were applied in the reduction of chalcones, T. absinthioides showed high chemoselectivity toward the CC double bond whereas the other two demonstrated abilities to biohydrogenate the CC double bounds and the carbonyl groups in a sequential fashion.
authors.affiliation: INTEQUI-CONICET, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, CP 5700 San Luis, Argentina.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Magallanes-Noguera
authors.affiliation: INTEQUI-CONICET, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, CP 5700 San Luis, Argentina.
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Cecati
authors.affiliation: IMIBIO-CONICET, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, Argentina.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Mascotti
authors.affiliation: INTEQUI-CONICET, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, CP 5700 San Luis, Argentina.
authors.forename: Guillermo F
authors.initials: GF
authors.lastname: Reta
authors.affiliation: Departamento de Biología Molecular, FCEFQN, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, CP 5800 Río Cuarto, Córdoba, Argentina.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Agostini
authors.affiliation: INTEQUI-CONICET, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, CP 5700 San Luis, Argentina. Electronic address: alejandroorden@gmail.com.
authors.forename: Alejandro A
authors.initials: AA
authors.lastname: Orden
authors.affiliation: INTEQUI-CONICET, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, CP 5700 San Luis, Argentina. Electronic address: marcelakurina@gmail.com.
authors.forename: Marcela
authors.initials: M
authors.lastname: Kurina-Sanz
mesh_terms: D019659:Asteraceae / Q000378:metabolism*; D055162:Biocatalysis; D002212:Capsicum / Q000378:metabolism*; D047188:Chalcones / Q000378:metabolism*; D046508:Culture Techniques; D006865:Hydrogenation; D000455:Medicago sativa / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism
chemical_list: D047188:Chalcones; D010940:Plant Proteins; D010088:Oxidoreductases
keywords: (E)-β-nitrostyrene (PubChem CID: 5284459); 1,3-diphenyl-2-propen-1-one (PubChem CID: 637760); 2-cyclohexene-1-one (PubChem CID: 13594); 2-methyl-2-cyclopenten-1-one (PubChem CID 14266); 3-methyl-2-cyclopenten-1-one (PubChem CID: 17691); Calli; Carbonyl reductase; Chalcone; Ene-reductase; Hairy roots; N-phenylmaleimide (PubChem CID: 13662); α,β-Unsaturated compound