title: Development of Recombinant Canarypox Viruses Expressing Immunogens.
abstract: Canarypox viruses (CNPV) are excellent candidates to develop recombinant vector vaccines due to both their capability to induce protective immune responses and their incompetence to replicate in mammalian cells (safety profile). In addition, CNPV and the derived recombinants can be manipulated under biosafety level 1 conditions. There is no commercially available system to obtain recombinant CNPV; however, the methodology and tools required to develop recombinant vaccinia virus (VV), prototype of the Poxviridae family, can be easily adapted. This chapter provides protocols for the generation, plaque isolation, molecular characterization, amplification and purification of recombinant CNPV.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto y de los Reseros, Hurlingham, Buenos Aires, Argentina.|Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield, 563, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Débora
authors.initials: D
authors.lastname: Garanzini
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto y de los Reseros, Hurlingham, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz, 2290, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: María Paula
authors.initials: MP
authors.lastname: Del Médico-Zajac
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto y de los Reseros, Hurlingham, Buenos Aires, Argentina. calamante.gabriela@inta.gob.ar.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calamante
mesh_terms: D000818:Animals; D028121:Canarypox virus / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D002460:Cell Line; D002645:Chickens; D005347:Fibroblasts / Q000276:immunology / Q000821:virology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: Canarypox; Homologous recombination; Nonessential region; Transfection; Transfer vector; Visual screening