title: Influence of antihypertensive drugs on aortic and coronary effects of Ang-(1-7) in pressure-overloaded rats.
abstract: This study investigated the influence of antihypertensive drugs, such as angiotensin-converting enzyme inhibitors (ACEIs), AT1 receptor blockers (ARBs), voltage-gated L-type calcium channel blockers, and mineralocorticoid receptor antagonists (MRAs), on the effects of angiotensin-(1-7) [Ang-(1-7)] on aorta and coronary arteries from pressure-overloaded rats. Pressure overload was induced by abdominal aortic banding (AB). To evaluate the role of antihypertensive drugs on the effect of Ang-(1-7), AB male Wistar rats weighing 250-300 g were treated with vehicle or low doses (5 mg·kg-1·day-1, gavage) of losartan, captopril, amlodipine, or spironolactone. Isolated aortic rings and isolated perfused hearts under constant flow were used to evaluate the effect of Ang-(1-7) in thoracic aorta and coronary arteries, respectively. Ang-(1-7) induced a significant relaxation in the aorta of sham animals, but this effect was reduced in the aortas of AB rats. Chronic treatments with losartan, captopril or amlodipine, but not with spironolactone, restored the Ang-(1-7)-induced aorta relaxation in AB rats. The coronary vasodilatation evoked by Ang-(1-7) in sham rats was blunted in hypertrophic rats. Only the treatment with losartan restored the coronary vasodilatory effect of Ang-(1-7) in AB rat hearts. These data support a beneficial vascular effect of an association of Ang-(1-7) and some antihypertensive drugs. Thus, this association may have potential as a new therapeutic strategy for cardiovascular diseases.
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: A D C
authors.initials: AD
authors.lastname: Nunes
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: A P S
authors.initials: AP
authors.lastname: Souza
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Macedo
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: P H
authors.initials: PH
authors.lastname: Alves
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: G R
authors.initials: GR
authors.lastname: Pedrino
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: D B
authors.initials: DB
authors.lastname: Colugnati
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.|Instituto Nacional de Ciência e Tecnologia em Nanobiofarmacêutica, Brasil.
authors.forename: E P
authors.initials: EP
authors.lastname: Mendes
authors.affiliation: Instituto Nacional de Ciência e Tecnologia em Nanobiofarmacêutica, Brasil.|Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: R A S
authors.initials: RA
authors.lastname: Santos
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.|Instituto Nacional de Ciência e Tecnologia em Nanobiofarmacêutica, Brasil.
authors.forename: C H
authors.initials: CH
authors.lastname: Castro
mesh_terms: D017311:Amlodipine / Q000494:pharmacology; D000803:Angiotensin I / Q000494:pharmacology*; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001012:Aorta, Abdominal / Q000187:drug effects*; D001794:Blood Pressure / Q000187:drug effects; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D003331:Coronary Vessels / Q000187:drug effects*; D019808:Losartan / Q000494:pharmacology; D008297:Male; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D023421:Models, Animal; D010446:Peptide Fragments / Q000494:pharmacology*; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013148:Spironolactone / Q000494:pharmacology; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D000451:Mineralocorticoid Receptor Antagonists; D010446:Peptide Fragments; D017311:Amlodipine; D013148:Spironolactone; D000803:Angiotensin I; D002216:Captopril; C118790:angiotensin I (1-7); D019808:Losartan