title: Metabolic Profiling of Cells in Response to Drug Treatment using1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy.
abstract: High-resolution magic angle spinning (HR-MAS) is an NMR technique that provides access to well resolved liquid-like 1H NMR spectra of semi-solid samples. Therefore, 1H HR-MAS NMR spectroscopy has become an important tool for the direct analysis of biological samples such as tissues and cells in a mostly non-destructive way. Here, we focus on the application of HR-MAS NMR combined with multivariate statistical methods used for metabolic profiling of cells and in particular for the study of cellular metabolic responses to drug exposure. The principles of HR-MAS and the metabolomic approach are briefly described. As an example, a study on the metabolic response of different cell types towards treatment with a highly cytotoxic hexacationic ruthenium metallaprism as potential anti-cancer drug is presented. Specific metabolites and metabolic pathways are suggested to be associated with the cellular response. The study demonstrates the potential of HR-MAS metabolomics applied to cells for addressing the intracellular processes involved in the treatment with organometallic drugs.
authors.affiliation: Department of Chemistry and Biochemistry University of Bern, Freiestrasse 3 CH-3012 Bern, Switzerland;, Email: martina.vermathen@dcb.unibe.ch.
authors.forename: Martina
authors.initials: M
authors.lastname: Vermathen
authors.affiliation: Departments of Clinical Research and Radiology, University and Inselspital Bern, Switzerland.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Diserens
authors.affiliation: Departments of Clinical Research and Radiology, University and Inselspital Bern, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Vermathen
authors.affiliation: Department of Chemistry and Biochemistry University of Bern, Freiestrasse 3 CH-3012 Bern, Switzerland;, Email: julien.furrer@dcb.unibe.ch.
authors.forename: Julien
authors.initials: J
authors.lastname: Furrer
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D025341:Principal Component Analysis; D012428:Ruthenium / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D012428:Ruthenium; D002945:Cisplatin