title: Improved biomass and lipid production in Synechocystis sp. NN using industrial wastes and nano-catalyst coupled transesterification for biodiesel production.
abstract: In this study, the improved biomass (1.6 folds) and lipid (1.3 folds) productivities in Synechocystis sp. NN using agro-industrial wastes supplementation through hybrid response surface methodology-genetic algorithm (RSM-GA) for cost-effective methodologies for biodiesel production was achieved. Besides, efficient harvesting in Synechocystis sp. NN was achieved by electroflocculation (flocculation efficiency 97.8±1.2%) in 10min when compared to other methods. Furthermore, different pretreatment methods were employed for lipid extraction and maximum lipid content of 19.3±0.2% by Synechocystis sp. NN was attained by ultrasonication than microwave and liquid nitrogen assisted pretreatment methods. The highest FAME (fatty acid methyl ester) conversion of 36.5±8.3mg FAME/g biomass was obtained using titanium oxide as heterogeneous nano-catalyst coupled whole-cell transesterification based method. Conclusively, Synechocystis sp. NN may be used as a biodiesel feedstock and its fuel production can be enriched by hybrid RSM-GA and nano-catalyst technologies.
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Kalimuthu
authors.initials: K
authors.lastname: Jawaharraj
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Rathinasamy
authors.initials: R
authors.lastname: Karpagam
authors.affiliation: Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Balasubramaniem
authors.initials: B
authors.lastname: Ashokkumar
authors.affiliation: Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Kathiresan
authors.affiliation: Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar 626001, Tamil Nadu, India.
authors.forename: Innasi Muthu Ganesh
authors.initials: IMG
authors.lastname: Moorthy
authors.affiliation: Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Muthu
authors.initials: M
authors.lastname: Arumugam
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India. Electronic address: pvlakshmi.biotech@mkuniversity.org.
authors.forename: Perumal
authors.initials: P
authors.lastname: Varalakshmi
mesh_terms: D056804:Biofuels; D018533:Biomass; D004951:Esterification; D007220:Industrial Waste; D008055:Lipids; D046939:Synechocystis
chemical_list: D056804:Biofuels; D007220:Industrial Waste; D008055:Lipids
keywords: Biodiesel; Electroflocculation; Hybrid RSM-GA; Nano-catalysts; Synechocystis sp.; Whole cell transesterification