title: Study of N-vinylpyrrolidone-N-vinylformamide copolymers labelled with indium-113m.
abstract: The procedure of the directed synthesis of N-vinylpyrrolidone-N-vinylformamide (VP-VFA) copolymers with grafted iminodiacetate (IDA) chelating units is presented. The methods for labelling resulting conjugates with indium-113m were developed. The metal-copolymer conjugates were characterized by different physicochemical methods, including IR and NMR, viscometry, light scattering, and exclusion high-performance liquid chromatography. Parameters of radiochemical synthesis of the conjugates labelled with indium-113m were optimized. It was shown that the VP-VFA-IDA copolymer firmly binds indium-113m both in the acid and alkaline solutions, with pH of the reaction mixture having almost no effect on the complexation. VP-VFA-IDA-In conjugates were found to be unstable in histidine challenge reaction.
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Gorshkov
authors.affiliation: Khlopin Radium Institute Joint-Stock Company, St Petersburg, Russia.|Radiochemistry Department, St Petersburg State University, St Petersburg, Russia.
authors.forename: А Е
authors.identifier: 0000-0003-0150-3040
authors.initials: АЕ
authors.lastname: Miroslavov
authors.affiliation: Khlopin Radium Institute Joint-Stock Company, St Petersburg, Russia.
authors.forename: I Е
authors.initials: IЕ
authors.lastname: Аlekseev
authors.affiliation: Khlopin Radium Institute Joint-Stock Company, St Petersburg, Russia.
authors.forename: А А
authors.initials: АА
authors.lastname: Lumpov
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: А Y
authors.initials: АY
authors.lastname: Мurko
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Gavrilova
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Saprykina
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: М А
authors.initials: МА
authors.lastname: Bezrukova
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: А I
authors.initials: АI
authors.lastname: Kipper
authors.affiliation: Institute of Macromolecular Compounds, Federal State Budgetary Institute of Science, Russian Academy of Sciences, St Petersburg, Russia.
authors.forename: V D
authors.initials: VD
authors.lastname: Krasikov
authors.affiliation: Khlopin Radium Institute Joint-Stock Company, St Petersburg, Russia.
authors.forename: D N
authors.initials: DN
authors.lastname: Suglobov
authors.affiliation: Khlopin Radium Institute Joint-Stock Company, St Petersburg, Russia.
authors.forename: M Y
authors.initials: MY
authors.lastname: Tyupina
authors.affiliation: St Petersburg Peter the Great Polytechnical Institutе of the Russian Federation, St Petersburg, Russia.
authors.forename: Е F
authors.initials: ЕF
authors.lastname: Panarin
mesh_terms: D005559:Formamides / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D007205:Indium Radioisotopes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011760:Pyrrolidinones / Q000737:chemistry*
chemical_list: D005559:Formamides; D007205:Indium Radioisotopes; C000615555:Indium-113m; D011108:Polymers; D011760:Pyrrolidinones; C031066:formamide; D006639:Histidine; C042670:N-vinyl-2-pyrrolidinone
keywords: N-vinylpyrrolidone-N-vinylformamide copolymer; bioconjugates; indium-113m; labelling; radiochemical synthesis; site specific radiopharmaceuticals