title: Effects of Lidocaine on HT-29 and SW480 Colon Cancer CellsIn Vitro.
abstract: Evidence is growing that the risk of cancer dissemination may be enhanced during the perioperative period. Whether particular anesthetic techniques influence oncological outcome is still under discussion. For pain management, lidocaine can be administered perioperatively by intravenous, intraperitoneal or epidural infusion. Here we investigated the effect of lidocaine on colon carcinoma cell lines (HT-29 and SW480) in vitro.
  
  
  
  ELISA BrdU (Roche) for cell proliferation and FITC Annexin V detection kit (BD Pharming) for apoptosis analysis were applied. Cell-cycle profiles were investigated by flow cytometry.
  
  
  
  Cell-cycle arrest was induced in both cell lines by 1000 μM lidocaine, while no inhibition of cell proliferation was detected. Apoptosis decreased in SW480 but not in HT-29 cells.
  
  
  
  Lidocaine induces cell-cycle arrest in both colon carcinoma cell lines in vitro. The effective drug concentration can be obtained by local infiltration.
authors.affiliation: Department of Anesthesiology, University of Regensburg, Regensburg, Germany anika.bundscherer@ukr.de.
authors.forename: Anika C
authors.initials: AC
authors.lastname: Bundscherer
authors.affiliation: Department of Anesthesiology, University of Regensburg, Regensburg, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Malsy
authors.affiliation: Department of Anesthesiology, University of Regensburg, Regensburg, Germany.
authors.forename: Diane I
authors.initials: DI
authors.lastname: Bitzinger
authors.affiliation: Department of Anesthesiology, Herzogin Elisabeth Hospital, Braunschweig, Germany.
authors.forename: Christoph H R
authors.initials: CH
authors.lastname: Wiese
authors.affiliation: Department of Anesthesiology, University of Regensburg, Regensburg, Germany.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gruber
authors.affiliation: Department of Anesthesiology, University of Regensburg, Regensburg, Germany.
authors.forename: Bernhard M
authors.initials: BM
authors.lastname: Graf
mesh_terms: D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008012:Lidocaine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000889:Anti-Arrhythmia Agents; D008012:Lidocaine
keywords: Lidocaine; apoptosis; cell cycle; cell proliferation; local anesthetics