title: Mitochondrial proteomics of the acetic acid - induced programmed cell death response in a highly tolerantZygosaccharomyces bailii- derived hybrid strain.
abstract: Very high concentrations of acetic acid at low pH induce programmed cell death (PCD) in both the experimental modelSaccharomyces cerevisiaeand inZygosaccharomyces bailii, the latter being considered the most problematic acidic food spoilage yeast due to its remarkable intrinsic resistance to this food preservative. However, while the mechanisms underlyingS. cerevisiaePCD induced by acetic acid have been previously examined, the corresponding molecular players remain largely unknown inZ. bailii. Also, the reason why acetic acid concentrations known to be necrotic forS. cerevisiaeinduce PCD with an apoptotic phenotype inZ. bailiiremains to be elucidated. In this study, a 2-DE-based expression mitochondrial proteomic analysis was explored to obtain new insights into the mechanisms involved in PCD in theZ. bailiiderived hybrid strain ISA1307. This allowed the quantitative assessment of expression of protein species derived from each of the parental strains, with special emphasis on the processes taking place in the mitochondria known to play a key role in acetic acid - induced PCD. A marked decrease in the content of proteins involved in mitochondrial metabolism, in particular, in respiratory metabolism (Cor1, Rip1, Lpd1, Lat1 and Pdb1), with a concomitant increase in the abundance of proteins involved in fermentation (Pdc1, Ald4, Dld3) was registered. Other differentially expressed identified proteins also suggest the involvement of the oxidative stress response, protein translation, amino acid and nucleotide metabolism, among other processes, in the PCD response. Overall, the results strengthen the emerging concept of the importance of metabolic regulation of yeast PCD.
authors.affiliation: Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.
authors.forename: Joana F
authors.initials: JF
authors.lastname: Guerreiro
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal. ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Belém
authors.initials: B
authors.lastname: Sampaio-Marques
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.
authors.forename: Renata
authors.initials: R
authors.lastname: Soares
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.
authors.forename: Ana V
authors.initials: AV
authors.lastname: Coelho
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal. ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Cecília
authors.initials: C
authors.lastname: Leão
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal. ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Ludovico
authors.affiliation: Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Sá-Correia
keywords: Zygosaccharomyces bailii; acetic acid response; mitochondrial proteomics; quantitative proteomics; yeast programmed cell death