title: Investigation of in vitro PDT activities of zinc phthalocyanine immobilised TiO2nanoparticles.
abstract: Phthalocyanines (Pcs) are commonly used as Photosensors (PSs) in Photodynamic Therapy (PDT) applications due to their intense absorption in the far red-near IR spectral region with a high extinction coefficient and high ability for generating singlet oxygen. Pcs targetspecifically tumors, and do not show any considerable toxic effects under the absence of light. In particular, their chemical versatility has allowed the introducion a number of substituent at the periferal or axial positions which provide modulating photophysical properties, increases the solubility of these compounds in organic solvents. Nanoparticles increase the bioavailability, stability, and transport of PSs to target tissue. TiO2nanoparticles are prefered in these applications because of their non toxic, low cost and high chemical stability properties. In our study, a Zinc Phthalocyanine (ZnPc) was used as a photosensor. The design of ZnPc integrated TiO2nanoparticles is intended to make PSs a more effective PDT agent. With the aim to examine the nuclear imaging/treatment potentials of ZnPc and ZnPc-TiO2in hepatocellular carcinoma (HepG2), colorectal adenocarcinoma (HT29) tumor and human healthy lung (WI38) cell lines in vitro study ZnPc and TiO2-ZnPc were also labeled with131I. It is determined that131I-ZnPc-TiO2nanoparticle show a potential as an agent for the imaging/treatment of hepatocellular cancer by in vitro. The toxicity studies revealed that TiO2nanoparticle decreases the toxicity of ZnPc. In vitro PDT results show that TiO2-ZnPc has a potential as a PDT agent in colon tumor treatment. Consequently, synthesized ZnPc and ZnPc-TiO2could be promising candidates as theranostic agents.
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100, Izmir, Turkey. Electronic address: ftmyurt@gmail.com.
authors.forename: Fatma
authors.initials: F
authors.lastname: Yurt
authors.affiliation: Department of Energy Systems Engineering, Mersin University, Faculty of Tarsus Technology, 33480 Mersin, Turkey. Electronic address: mine.ince79@gmail.com.
authors.forename: Mine
authors.initials: M
authors.lastname: Ince
authors.affiliation: Advanced Technology Reseach & Application Center, Mersin University, Ciftlikkoy Campus, TR33343, Yenisehir, Mersin, Turkey.
authors.forename: Suleyman Gokhan
authors.initials: SG
authors.lastname: Colak
authors.affiliation: Department of Energy Systems Engineering, Mersin University, Faculty of Tarsus Technology, 33480 Mersin, Turkey; Advanced Technology Reseach & Application Center, Mersin University, Ciftlikkoy Campus, TR33343, Yenisehir, Mersin, Turkey.
authors.forename: Kasim
authors.initials: K
authors.lastname: Ocakoglu
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Er
authors.affiliation: Department of Biomedical Technology, Institute of Science, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Hale Melis
authors.initials: HM
authors.lastname: Soylu
authors.affiliation: Department of MedicalBiology, Faculty of Medicine, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gunduz
authors.affiliation: Department of MedicalBiology, Faculty of Medicine, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Cigir Biray
authors.initials: CB
authors.lastname: Avci
authors.affiliation: Department of MedicalBiology, Faculty of Medicine, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Cansu Caliskan
authors.initials: CC
authors.lastname: Kurt
mesh_terms: D045744:Cell Line, Tumor; D019073:HT29 Cells; D056945:Hep G2 Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D053758:Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C009495:titanium dioxide; D014025:Titanium
keywords: Nuclear imaging; Photodynamic therapy; Titanium nanoparticle; Zinc phthalocyanine