title: Effects of Ag Nanoparticles on Growth and Fat Body Proteins in Silkworms (Bombyx mori).
abstract: Ag nanoparticles (AgNPs), a widely used non-antibiotic, antibacterial material, have shown toxic and other potentially harmful effects in mammals. However, the deleterious effects of AgNPs on insects are still unknown. Here, we studied the effects of AgNPs on the model invertebrate organism Bombyx mori. After feeding silkworm larvae different concentrations of AgNPs, we evaluated the changes of B. mori body weights, survival rates, and proteomic differences. The results showed that low concentrations (<400 mg/L) of AgNPs promoted the growth and cocoon weights of B. mori. Although high concentrations (≥800 mg/L) of AgNPs also improved B. mori growth, they resulted in silkworm death. An analysis of fat body proteomic differences revealed 13 significant differences in fat body protein spots, nine of which exhibited significantly downregulated expression, while four showed significantly upregulated expression. Reverse transcription-polymerase chain reaction results showed that at an AgNP concentration of 1600 mg/L, the expression levels of seven proteins were similar to the transcription levels of their corresponding genes. Our results suggest that AgNPs lowered﻿ ﻿the resistance to oxidative stress, affected cell apoptosis, and induced cell necrosis by regulating related protein metabolism and metabolic pathways in B. mori.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Nouara
authors.initials: N
authors.lastname: Abdlli
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Niannian
authors.initials: N
authors.lastname: Wang
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lü
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Nie
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Lu
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China. kpchen@ujs.edu.cn.
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D012831:Bombyx / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000074341:Dietary Exposure / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D005216:Fat Body / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D019476:Insect Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009336:Necrosis; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D040901:Proteomics / Q000379:methods; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D016019:Survival Analysis; D015430:Weight Gain / Q000187:drug effects
chemical_list: D004785:Environmental Pollutants; D019476:Insect Proteins; D012834:Silver
keywords: AgNPs; Bombyx mori; Environmental risk; Fat body proteins; Growth