title: Biocompatible 3D SERS substrate for trace detection of amino acids and melamine.
abstract: A novel, low-cost and biocompatible three-dimensional (3D) substrate for surface-enhanced Raman spectroscopy (SERS) is fabricated using gold nanoparticles (AuNPs) loaded on cellulose paper for detection of amino acids and melamine. Dysosma pleiantha rhizome (Dp-Rhi) capped AuNPs (Dp-Rhi_AuNPs) were prepared by in situ using aqueous extract of Dp-Rhi and in situ functionalized Dp-Rhi on AuNPs surface was verified by Fourier transform infrared spectroscopy and zeta potentials analysis shows a negative (-18.4mV) surface charges, which confirm that presence of Dp-Rhi on AuNPs. The biocompatibility of Dp-Rhi_AuNPs is also examined by cell viability of FaDu cells using MTS assay and compared to control group. In conclusion, the SERS performance of AuNPs@cellulose paper substrates were systematically demonstrated and examined with different excitation wavelengths (i.e. 532, 632.8 and 785nm lasers) and the as-prepared 3D substrates provided an enhancement factor approaching 7 orders of magnitude compared with conventional Raman intensity using para-nitrothiophenol (p-NTP), para-aminothiophenol (p-ATP) and para-mercaptobenzoic acid (p-MBA) as probe molecules. The strong electromagnetic effect was generated at the interface of AuNPs and pre-treated roughened cellulose paper is also investigated by simulation in which the formation of possible Raman hot-spot zone in fiber-like microstructure of cellulose paper decorated with AuNPs. Notably, with optimized condition of as-prepared 3D AuNPs@cellulose paper is highly sensitive in the SERS detection of aqueous tyrosine (10-10M) and melamine (10-9M).
authors.affiliation: Promotion Center for Global Materials Research (PCGMR), Department of Material Science and Engineering, National Cheng Kung University, Tainan, Taiwan. Electronic address: analyticalsathi@gmail.com.
authors.forename: Elumalai
authors.initials: E
authors.lastname: Satheeshkumar
authors.affiliation: Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan.
authors.forename: Palaniyandi
authors.initials: P
authors.lastname: Karuppaiya
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Kundan
authors.initials: K
authors.lastname: Sivashanmugan
authors.affiliation: Department of Life Science Research Center, Tunghai University, Taichung, Taiwan.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chao
authors.affiliation: Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan.
authors.forename: Hsin-Sheng
authors.initials: HS
authors.lastname: Tsay
authors.affiliation: Promotion Center for Global Materials Research (PCGMR), Department of Material Science and Engineering, National Cheng Kung University, Tainan, Taiwan. Electronic address: yoshimura@msl.titech.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshimura
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D028141:Berberidaceae / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D002482:Cellulose; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014227:Triazines / Q000032:analysis*
chemical_list: D000596:Amino Acids; D001672:Biocompatible Materials; D010936:Plant Extracts; D014227:Triazines; D006046:Gold; D002482:Cellulose; C011907:melamine
keywords: Biocompatibility; Cellulose paper; Gold nanoparticles; Melamine; Surface-enhanced Raman spectroscopy (SERS); Tyrosine