title: Microwave-Assisted Hydrothermal Rapid Synthesis of Calcium Phosphates: Structural Control and Application in Protein Adsorption.
abstract: Synthetic calcium phosphate (CaP)-based materials have attracted much attention in the biomedical field. In this study, we have investigated the effect of pH values on CaP nanostructures prepared using a microwave-assisted hydrothermal method. The hierarchical nanosheet-assembled hydroxyapatite (HAP) nanostructure was prepared under weak acidic conditions (pH 5), while the HAP nanorod was prepared under neutral (pH 7) and weak alkali (pH 9) condition. However, when the pH value increases to 11, a mixed product of HAP nanorod and tri-calcium phosphate nanoparticle was obtained. The results indicated that the pH value of the initial reaction solution played an important role in the phase and structure of the CaP. Furthermore, the protein adsorption and release performance of the as-prepared CaP nanostructures were investigated by using hemoglobin (Hb) as a model protein. The sample that was prepared at pH = 11 and consisted of mixed morphologies of nanorods and nanoprisms showed a higher Hb protein adsorption capacity than the sample prepared at pH 5, which could be explained by its smaller size and dispersed structure. The results revealed the relatively high protein adsorption capacity of the as-prepared CaP nanostructures, which show promise for applications in various biomedical fields such as drug delivery and protein adsorption.
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. caizhuyun@126.com.
authors.forename: Zhu-Yun
authors.initials: ZY
authors.lastname: Cai
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. maspf@163.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Peng
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. ziyp0935@163.com.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Zi
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. fchen@mail.sic.ac.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. qianqr@163.com.
authors.forename: Qi-Rong
authors.initials: QR
authors.lastname: Qian
keywords: drug delivery; hydroxyapatite; microwave; nanostructure; protein adsorption