title: Functional Salivary Gland Regeneration.
abstract: The salivary gland plays important roles in maintaining the health and homeostasis of the oral cavity by regulating functions, such as chewing, digestion, cleaning, and swallowing. Salivary gland dysfunction causes dry mouth syndrome, which includes many oral problems, such as dental caries, bacterial infection, mastication dysfunction, and swallowing dysfunction. Therefore, salivary gland regeneration is expected, and we attempted to establish a method by manual approaches. Previously, a novel three-dimensional cell manipulation technique, the "organ germ method," to create a bioengineered organ germ enabled the regeneration of structurally correct and fully functional bioengineered organs, including the teeth, hair follicles, and secretory glands, in vivo. Here, we describe the protocol for salivary gland germ regeneration using this method and the functional analysis of the regenerated salivary gland.
authors.affiliation: Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe, Hyogo, 650-0047, Japan.|Organ Technologies Inc., Minato-ku, Tokyo, 101-0048, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe, Hyogo, 650-0047, Japan. t-tsuji@cdb.riken.jp.|Organ Technologies Inc., Minato-ku, Tokyo, 101-0048, Japan. t-tsuji@cdb.riken.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods; D012469:Salivary Glands / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D014070:Tooth / Q000166:cytology
keywords: Bioengineered salivary gland germ; Organ germ method; Saliva secretion; Salivary gland; Transplantation