title: Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications.
abstract: The aim of this study was to develop an ultra-fast molecular detection method for meat identification using convection Palm polymerase chain reaction (PCR). The mitochondrial cytochrome b (Cyt b) gene was used as a target gene. Amplicon size was designed to be different for beef, lamb, and pork. When these primer sets were used, each species-specific set specifically detected the target meat species in singleplex and multiplex modes in a 24min PCR run. The detection limit was 1pg of DNA for each meat species. The convection PCR method could detect as low as 1% of meat adulteration. The stability of the assay was confirmed using thermal processed meats. We also showed that direct PCR can be successfully performed with mixed meats and food samples. These results suggest that the developed assay may be useful in the authentication of meats and meat products in laboratory and rapid on-site applications.
authors.affiliation: R&D Center, Ahram Biosystems Inc., Seoul 133-120, Republic of Korea; Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Kyung-Young
authors.initials: KY
authors.lastname: Song
authors.affiliation: R&D Center, Ahram Biosystems Inc., Seoul 133-120, Republic of Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Hwang
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Oral Biochemistry and Molecular Biology, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: jhkimh@khu.ac.kr.
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA
keywords: Convection PCR; DNA-based; Meat species; Multiplex