title: Identification of oxidoreductases from the petroleumBacillus safensisstrain.
abstract: A gram-positive bacterium, denominated CFA-06, was isolated from Brazilian petroleum in the Campos Basin and is responsible for the degradation of aromatic compounds and petroleum aromatic fractions. The CFA-06 strain was identified asBacillus safensisusing the 16S rRNA and gyrase B sequence. Enzymatic assays revealed the presence of two oxidoreductases: a catalase and a new oxidoreductase. The oxidoreductases were enzymatically digested and analyzed via ESI-LTQ-Orbitrap mass spectrometry. The mass data revealed a novel oxidoreductase (named BsPMO) containing 224 amino acids and 89% homology with a hypothetic protein fromB. safensis(CFA-06) and a catalase (named BsCat) with 491 amino acids and 60% similarity with the catalase fromBacillus pumilus(SAFR-032). The new protein BsPMO contains iron atom(s) and shows catalytic activity toward a monooxygenase fluorogenic probe in the presence of cofactors (NADH, NADPH and NAD). This study enhances our knowledge of the biodegradation process of petroleum byB. safensis.
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil; Institute of Agricultural Sciences, Federal University of Minas Gerais, 39404-547 Minas Gerais, Brazil.
authors.forename: Francine S A
authors.initials: FS
authors.lastname: da Fonseca
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Célio F F
authors.initials: CF
authors.lastname: Angolini
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Marco A Zezzi
authors.initials: MA
authors.lastname: Arruda
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Cícero A L
authors.initials: CA
authors.lastname: Junior
authors.affiliation: Center for Molecular Biology and Genetic Engineering, University of Campinas, 13083-875 São Paulo, Brazil.
authors.forename: Clelton A
authors.initials: CA
authors.lastname: Santos
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil; National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil.
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Saraiva
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Pilau
authors.affiliation: Center for Molecular Biology and Genetic Engineering, University of Campinas, 13083-875 São Paulo, Brazil.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
authors.affiliation: Center for Molecular Biology and Genetic Engineering, University of Campinas, 13083-875 São Paulo, Brazil.
authors.forename: Prianda R
authors.initials: PR
authors.lastname: Laborda
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas, CP 6171, CEP 13081-970, Campinas, SP, Brazil.
authors.forename: Patrícia F L
authors.initials: PF
authors.lastname: de Oliveira
authors.affiliation: Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas, CP 6171, CEP 13081-970, Campinas, SP, Brazil.
authors.forename: Valéria M
authors.initials: VM
authors.lastname: de Oliveira
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Francisco de Assis M
authors.initials: FA
authors.lastname: Reis
authors.affiliation: Chemistry Institute, University of Campinas, P.B 6154, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Marsaioli
keywords: Bacillus safensis; Catalase; Monooxygenase