title: DNA Three-Way Junction for Differentiation of Single-Nucleotide Polymorphisms with Fluorescent Copper Nanoparticles.
abstract: A label- and enzyme-free fluorescent sensor for the detection of single-nucleotide polymorphisms (SNPs) at room temperature is proposed, using new copper nanoparticles (CuNPs) as fluorescent reporters. The CuNPs were constructed by using a DNA three-way junction (3WJ) template. In this assay, two complementary adenine/thymine-rich probes can hybridize with the wild-type target simultaneously to construct a 3WJ structure, serving as an efficient scaffold for the generation of CuNPs. However, the CuNPs produce weak fluorescence when the probes bind with a mutant-type target. SNPs can be identified by the difference in fluorescence intensity of the CuNPs. This SNPs detection strategy is straightforward, cost-effective, and avoids the complicated procedures of labeling or enzymatic reactions. The fluorescent sensor is versatile and can be applied to all types of mutation because the probes are programmable. Moreover, the sensor exhibits good detection performance in biological samples.
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Sun
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.|High School Affiliated to Southwest University, Chongqing, 400700, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: You
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Petroleum Pollution Control, Beijing, 102206, P. R. China.|CNPC Research Institute of Safety and Environmental Technology, Beijing, 102206, P. R. China.
authors.forename: Quanwei
authors.initials: Q
authors.lastname: Song
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Shen
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Na
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
authors.forename: Jin
authors.identifier: http://orcid.org/0000-0002-0286-0798
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D003300:Copper; D004247:DNA
keywords: DNA three-way junction; copper; fluorescence; nanoparticles; single-nucleotide polymorphisms