title: L-Threonine and its analogue added to autoclaved solid medium suppress trichothecene production by Fusarium graminearum.
abstract: Fusarium graminearum produces trichothecene mycotoxins under certain nutritional conditions. When L-Thr and its analogue L-allo-threonine were added to brown rice flour solid medium before inoculation, trichothecene production after 4 days of incubation was suppressed. A time-course analysis of gene expression demonstrated that L-Thr suppressed transcription of Tri6, a trichothecene master regulator gene, and a terpene cyclase Tri5 gene. Regulation of trichothecene biosynthesis by altering major primary metabolic processes may open up the possibility to develop safe chemicals for the reduction of mycotoxin contamination might be developed.
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Maeda
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nakajima
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tanahashi
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kitou
authors.affiliation: Division of Functional Genomics, Advanced Science Research Centre, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Miwa
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kanamaru
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Division of Functional Genomics, Advanced Science Research Centre, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Nishiuchi
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan. mkimura@agr.nagoya-u.ac.jp.
authors.forename: Makoto
authors.identifier: http://orcid.org/0000-0001-5310-2893
authors.initials: M
authors.lastname: Kimura
mesh_terms: D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D005670:Fusarium / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D006358:Hot Temperature; D009183:Mycotoxins / Q000096:biosynthesis*; D012275:Oryza / Q000382:microbiology; D013912:Threonine / Q000737:chemistry / Q000378:metabolism*; D014255:Trichothecenes / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D009183:Mycotoxins; D014255:Trichothecenes; D013912:Threonine
keywords: Amino acids; Fusarium graminearum; Mycotoxin production inhibitor; Nitrogen source; Tri gene expression