title: Changes in the number of CD31-CD45-Sca-1+cells and Shh signaling pathway involvement in the lungs of mice with emphysema and relevant effects of acute adenovirus infection.
abstract: BACKGROUND
  COPD is a leading cause of mortality worldwide, and cigarette smoke is a pivotal risk factor. Adenovirus is a common cause of acute exacerbations of COPD and expedites COPD progression. Lung stem/progenitor cells play an important role in the development of COPD, while the relevant mechanism remains elusive. Here, we investigated the number of lung CD31-CD45-Sca-1+cells and sonic hedgehog (Shh) signaling pathway expression levels in cigarette smoke extract (CSE)-induced emphysema mice, as well as the relevant effects of acute adenovirus infection (AAI).
  
  
  METHODS
  BALB/c mice were treated with CSE by intraperitoneal injection and/or adenovirus endotracheal instillation at different time points for 28 days. Lung function, lung histomorphology, CD31-CD45-Sca-1+cell count, and expression levels of major components in the Shh signaling pathway in the lungs were measured.
  
  
  RESULTS
  CSE intraperitoneal injection and adenovirus endotracheal instillation successfully induced emphysema and AAI in mice, respectively. In the lungs of emphysema mice, both the number of CD31-CD45-Sca-1+cells and expression levels of Shh signaling pathway molecules were reduced. However, AAI increased the number of inhibited CD31-CD45-Sca-1+cells and activated the suppression of the Shh signaling pathway.
  
  
  CONCLUSIONS
  Both CD31-CD45-Sca-1+cell numbers and Shh signaling pathway expression levels were downregulated in the lungs of emphysema mice induced by CSE intraperitoneal injection, which likely contributes to the pathogenesis of emphysema. Additionally, these inhibited lung CD31-CD45-Sca-1+cells and Shh signaling pathway molecules were upregulated during AAI, indicating that they play a protective role in the epithelial repair process after AAI injury.
authors.affiliation: Respiratory Medicine Department, PLA Rocket Force General Hospital, Beijing; Respiratory Medicine Department.
authors.forename: Minhua
authors.initials: M
authors.lastname: Deng
authors.affiliation: Respiratory Medicine Department.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Rehabilitation Department, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Respiratory Medicine Department.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Respiratory Medicine Department.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
mesh_terms: D000257:Adenoviridae Infections / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D005786:Gene Expression Regulation; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D000072081:Patched-1 Receptor / Q000235:genetics / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011656:Pulmonary Emphysema / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D015398:Signal Transduction; D012906:Smoke / Q000009:adverse effects; D012907:Smoking / Q000009:adverse effects; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D000071676:Zinc Finger Protein GLI1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; C498520:Gli1 protein, mouse; D053823:Hedgehog Proteins; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D000072081:Patched-1 Receptor; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C000606240:Ptch1 protein, mouse; D012333:RNA, Messenger; C465570:Shh protein, mouse; D012906:Smoke; D000071676:Zinc Finger Protein GLI1; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse
keywords: adenovirus; emphysema; lung CD31−CD45−Sca-1+ cells; sonic hedgehog signaling pathway; stem cells