title: A New Kinetic Spectrophotometric Method for the Quantitation of Amorolfine.
abstract: Amorolfine (AOF) is a compound with fungicide activity based on the dual inhibition of growth of the fungal cell membrane, the biosynthesis and accumulation of sterols, and the reduction of ergosterol. In this work a sensitive kinetic and spectrophotometric method for the AOF quantitation based on the AOF oxidation by means of KMnO4at 30 min (fixed time), pH alkaline, and ionic strength controlled was developed. Measurements of changes in absorbance at 610 nm were used as criterion of the oxidation progress. In order to maximize the sensitivity, different experimental reaction parameters were carefully studied via factorial screening and optimized by multivariate method. The linearity, intraday, and interday assay precision and accuracy were determined. The absorbance-concentration plot corresponding to tap water spiked samples was rectilinear, over the range of 7.56 × 10-6-3.22 × 10-5mol L-1, with detection and quantitation limits of 2.49 × 10-6mol L-1and 7.56 × 10-6mol L-1, respectively. The proposed method was successfully validated for the application of the determination of the drug in the spiked tap water samples and the percentage recoveries were 94.0-105.0%. The method is simple and does not require expensive instruments or complicated extraction steps of the reaction product.
authors.affiliation: Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.
authors.forename: César
authors.identifier: 0000-0002-4446-0557
authors.initials: C
authors.lastname: Soto
authors.affiliation: Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.
authors.forename: Cristian
authors.initials: C
authors.lastname: Poza
authors.affiliation: Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.
authors.forename: David
authors.initials: D
authors.lastname: Contreras
authors.affiliation: Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Yáñez
authors.affiliation: Laboratorio de Química Analítica, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: Fallon
authors.initials: F
authors.lastname: Nacaratte
authors.affiliation: Laboratorio de Química Analítica, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: M Inés
authors.initials: MI
authors.lastname: Toral