title: Ribosome Profiling for the Analysis of Translation During Yeast Meiosis.
abstract: Ribosome profiling provides a genome-wide view of translation with unprecedented resolution. Application of this approach to fission and budding yeast revealed widespread regulation of translational efficiency, translation of short open reading frames on unannotated transcripts, and frequent translation of open reading frames in 5' leader sequences. We present here a detailed protocol for the application of ribosome profiling to meiotic fission yeast cells, although the approach should be easily adapted to budding yeast.
authors.affiliation: Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge, CB2 1QW, UK.
authors.forename: Caia
authors.initials: C
authors.lastname: Duncan
authors.affiliation: Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge, CB2 1QW, UK. jm593@cam.ac.uk.
authors.forename: Juan
authors.initials: J
authors.lastname: Mata
mesh_terms: D008540:Meiosis; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012331:RNA, Fungal / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D012270:Ribosomes / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; C087019:ran1 protein, S pombe; D017346:Protein Serine-Threonine Kinases
keywords: Meiosis; NGS; Protein synthesis; Ribo-seq; Ribosome profiling; S. pombe; Translation