title: Impact of Cytological Sampling on EGFR Mutation Testing in Stage III-IV Lung Adenocarcinoma.
abstract: Objectives. There have been advances in the identification and understanding of molecular subsets of lung cancer, defined by specific oncogenic aberrations. A number of actionable genetic alterations have been identified, such as the epidermal growth factor receptor (EGFR) mutation. We aimed to establish the reasons why patients were not undergoing EGFR mutation testing at the time of histological diagnosis.Methods. The records of 70 patients with advanced adenocarcinoma of the lung managed through a single multidisciplinary team at a single institution were reviewed. Data were collected on method of tumour sample collection, whether this was sent for EGFR testing, and the result.Results. Seventy patients were identified. In 21/25 (84%) cases, cytological sampling was sufficient for EGFR mutation analysis, compared with 40/45 (89%) cases with histological sampling. EGFR mutation testing was not carried out in 22/70 (31.4%) patients. There was insufficient tumour sample for EGFR testing in 9/22 (40.9%) patients. Other reasons for not testing included poor patient fitness and problems in the diagnostic pathway.Conclusions. In this series, cytological tumour sampling was not the predominant reason why cancers failed to have EGFR mutation status established.
authors.affiliation: Velindre Cancer Centre, Cardiff, UK.
authors.forename: Rhian Siân
authors.identifier: 0000-0002-2060-3357
authors.initials: RS
authors.lastname: Davies
authors.affiliation: Velindre Cancer Centre, Cardiff, UK.
authors.forename: Christian
authors.initials: C
authors.lastname: Smith
authors.affiliation: Velindre Cancer Centre, Cardiff, UK.
authors.forename: Gwenllian
authors.initials: G
authors.lastname: Edwards
authors.affiliation: Institute of Medical Genetics, University Hospital of Wales, Cardiff, UK.
authors.forename: Rachel
authors.initials: R
authors.lastname: Butler
authors.affiliation: University Hospital Llandough, Cardiff, UK.
authors.forename: Diane
authors.initials: D
authors.lastname: Parry
authors.affiliation: Velindre Cancer Centre, Cardiff, UK.
authors.forename: Jason Francis
authors.initials: JF
authors.lastname: Lester