title: A Novel Organic Selenium Compound Exerts Unique Regulation of Selenium Speciation, Selenogenome, and Selenoproteins in Broiler Chicks.
abstract: Background:A new organic selenium compound, 2-hydroxy-4-methylselenobutanoic acid (SeO), displayed a greater bioavailability than sodium selenite (SeNa) or seleno-yeast (SeY) in several species.Objective:This study sought to determine the regulation of the speciation of selenium, expression of selenogenome and selenocysteine biosynthesis and degradation-related genes, and production of selenoproteins by the 3 forms of selenium in the tissues of broiler chicks.Methods:Day-old male chicks (n= 6 cages/diet, 6 chicks/cage) were fed a selenium-deficient, corn and soy-based diet [base diet (BD), 0.05 mg Se/kg] or the BD + SeNa, SeY, or SeO at 0.2 mg Se/kg for 6 wk. Plasma, livers, and pectoral and thigh muscles were collected at weeks 3 and 6 to assay for total selenium, selenomethionine, selenocysteine, redox status, and selected genes, proteins, and enzymes.Results:Although both SeY and SeO produced greater concentrations (P< 0.05) of total selenium (20-172%) and of selenomethionine (≤15-fold) in the liver, pectoral muscle, and thigh than those of SeNa, SeO further raised (P< 0.05) these concentrations by 13-37% and 43-87%, respectively, compared with SeY. Compared with the BD, only SeO enhanced (P <0.05) the mRNA ofselenoprotein(Seleno)sandmethionine sulfoxide reductase B1(Msrb1) in the liver and thigh (62-98%) and thioredoxin reductase (TXRND) activity in the pectoral and thigh muscles (20-37%) at week 3. Furthermore, SeO increased (P <0.05) the expression ofglutathione peroxidase(Gpx)3, GPX4, SELENOP, and SELENOU relative to the SeNa group by 26-207%, and the expression ofSelenop, O-phosphoseryl-transfer RNA (tRNA):selenocysteinyl-tRNA synthase, GPX4, and SELENOP relative to the SeY group by 23-55% in various tissues.Conclusions:Compared with SeNa or SeY, SeO demonstrated a unique ability to enrich selenomethionine and total selenium depositions, to induce the early expression ofSelenosandMrsb1mRNA and TXRND activity, and to enhance the protein production of GPX4, SELENOP, and SELENOU in the tissues of chicks.
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China; lvhuisun@mail.hzau.edu.cn xl20@cornell.edu.
authors.forename: Lv-Hui
authors.initials: LH
authors.lastname: Sun
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, China.
authors.forename: Jia-Qiang
authors.initials: JQ
authors.lastname: Huang
authors.affiliation: Adisseo France S.A.S., Antony, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Briens
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Qi
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin, China; and.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Xu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, China; lvhuisun@mail.hzau.edu.cn xl20@cornell.edu.|Department of Animal Science, Cornell University, Ithaca, NY.
authors.forename: Xin Gen
authors.initials: XG
authors.lastname: Lei
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002087:Butyrates / Q000378:metabolism / Q000494:pharmacology*; D002645:Chickens; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051150:Methionine Sulfoxide Reductases / Q000235:genetics / Q000378:metabolism; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012643:Selenium / Q000172:deficiency / Q000378:metabolism*; D018036:Selenium Compounds / Q000378:metabolism / Q000494:pharmacology*; D012645:Selenomethionine / Q000378:metabolism*; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism*; D018038:Sodium Selenite / Q000494:pharmacology; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D015003:Yeasts
chemical_list: C551033:2-hydroxy-4-methylselenobutanoic acid; D002087:Butyrates; D012333:RNA, Messenger; D018036:Selenium Compounds; D051140:Selenoproteins; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D013880:Thioredoxin-Disulfide Reductase; D051150:Methionine Sulfoxide Reductases; D000604:Amino Acyl-tRNA Synthetases; D012643:Selenium; D018038:Sodium Selenite
keywords: chick; gene expression; selenium; selenoprotein; speciation