title: System-level perturbations of cell metabolism using CRISPR/Cas9.
abstract: CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to single and multiplex pathway modifications and transcriptional regulations. The effectiveness of these tools allows researchers to implement genome-wide perturbations, test model-guided genome editing strategies, and perform transcriptional reprogramming perturbations in a more advanced manner than previously possible. In this mini-review we highlight recent studies adopting CRISPR/Cas9 for systems-level perturbations and model-guided metabolic engineering.
authors.affiliation: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark.
authors.forename: Tadas
authors.initials: T
authors.lastname: Jakočiūnas
authors.affiliation: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark. Electronic address: mije@biosustian.dtu.dk.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Jensen
authors.affiliation: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark; Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Department of Chemical and Biomolecular Engineering & Department of Bioengineering University of California, Berkeley, CA, USA.
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D016678:Genome; D060847:Metabolic Engineering; D049490:Systems Biology / Q000379:methods*