title: A new pyrido[1,2-a]benzimidazole-rhodamine FRET system as an efficient ratiometric fluorescent probe for Cu2+in living cells.
abstract: A novel fluorophore pyrido[1,2-a]benzimidazole was synthesized and used as a donor to construct FRET system. The probe shows a large Stokes shifts (190 nm), high selectivity, fast response (within 2 min), and high sensitivity toward Cu2+(detection limit 42 nM). A significant colour change from colourless to pink could be observed, enabling naked-eye detection of Cu2+. Furthermore, it could be used for imaging Cu2+in living cells which demonstrated its value of practical applications in biological systems.
authors.affiliation: School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Ge
authors.affiliation: School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Ji
authors.affiliation: School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China.
authors.forename: Shili
authors.initials: S
authors.lastname: Shen
authors.affiliation: School of Chemical Engineering, Taishan Medical University, Taian, Shandong 271016, PR China. Electronic address: xiaoquncao@126.com.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Cao
mesh_terms: D001562:Benzimidazoles; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D012235:Rhodamines
chemical_list: D001562:Benzimidazoles; D005456:Fluorescent Dyes; D012235:Rhodamines; D003300:Copper
keywords: Bioimaging; Cu(2+); FRET; Fluorescent probe; Pyrido[1,2-a]benzimidazole