title: Investigation of ac-magnetic field stimulated nanoelectroporation of magneto-electric nano-drug-carrier inside CNS cells.
abstract: In this research, we demonstrate cell uptake of magneto-electric nanoparticles (MENPs) through nanoelectroporation (NEP) using alternating current (ac)-magnetic field stimulation. Uptake of MENPs was confirmed using focused-ion-beam assisted transmission electron microscopy (FIB-TEM) and validated by a numerical simulation model. The NEP was performed in microglial (MG) brain cells, which are highly sensitive for neuro-viral infection and were selected as target for nano-neuro-therapeutics. When the ac-magnetic field optimized (60 Oe at 1 kHz), MENPs were taken up by MG cells without affecting cell health (viability > 92%). FIB-TEM analysis of porated MG cells confirmed the non-agglomerated distribution of MENPs inside the cell and no loss of their elemental and crystalline characteristics. The presented NEP method can be adopted as a part of future nanotherapeutics and nanoneurosurgery strategies where a high uptake of a nanomedicine is required for effective and timely treatment of brain diseases.
authors.affiliation: Center for Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, USA.
authors.forename: Ajeet
authors.initials: A
authors.lastname: Kaushik
authors.affiliation: Center for Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, USA.
authors.forename: Roozbeh
authors.initials: R
authors.lastname: Nikkhah-Moshaie
authors.affiliation: Department of Electrical and Computing Engineering, Florida International University, Miami, USA.
authors.forename: Raju
authors.initials: R
authors.lastname: Sinha
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, USA.
authors.forename: Vinay
authors.initials: V
authors.lastname: Bhardwaj
authors.affiliation: Center for Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, USA.
authors.forename: Venkata
authors.initials: V
authors.lastname: Atluri
authors.affiliation: Center for Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, USA.
authors.forename: Rahul Dev
authors.initials: RD
authors.lastname: Jayant
authors.affiliation: Center for Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, USA.
authors.forename: Adriana
authors.initials: A
authors.lastname: Yndart
authors.affiliation: National Center for NanoBioElectronics, West Hollywood; California Neurosurgical Institute, Los Angeles; Brain Mapping Foundation, West Hollywood; Society for Brain Mapping and Therapeutics, West Hollywood, CA, USA.
authors.forename: Babak
authors.initials: B
authors.lastname: Kateb
authors.affiliation: Department of Electrical and Computing Engineering, Florida International University, Miami, USA.
authors.forename: Nezih
authors.initials: N
authors.lastname: Pala
authors.affiliation: Center for Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, USA.
authors.forename: Madhavan
authors.initials: M
authors.lastname: Nair
mesh_terms: D001921:Brain / Q000187:drug effects*; D002460:Cell Line; D004337:Drug Carriers; D004560:Electricity; D018274:Electroporation / Q000379:methods; D006801:Humans; D060526:Magnetic Fields; D017628:Microglia / Q000187:drug effects*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D004337:Drug Carriers