title: Involvement of Normalized Glial Fibrillary Acidic Protein Expression in the Hippocampi in Antidepressant-Like Effects of Xiaoyaosan on Chronically Stressed Mice.
abstract: The research has only yielded a partial comprehension of MDD and the mechanisms underlying the antidepressant-like effects of XYS. Therefore, in this study, we aimed to explore the effects of XYS on chronic unpredictable mild stress- (CUMS-) induced changes in the neuronal and the astrocytic markers in the mouse hippocampus. The physical states and depressive-like behaviors in mice with CUMS were recorded. The serum contents of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were measured. The protein and mRNA expressions and the immunoreactivities of glial fibrillary acidic protein (GFAP) and neuronal nuclei (NeuN) in mouse hippocampus were detected using a Western blot, qRT-PCR, and immunohistochemical staining, respectively. XYS treatment markedly improved the physical state and depressive-like behaviors in mice subjected to CUMS compared with the model group, and the serum contents of BDNF and GDNF were significantly upregulated. XYS treatment also elevated the protein and mRNA levels, as well as the immunoreactivity of GFAP in the hippocampus. However, CUMS did not influence NeuN expression. In conclusion, these results reveal that chronic administration of XYS elicits antidepressant-like effects in a mouse model of depression and may normalize glial fibrillary acidic protein expression in the hippocampi of mice with CUMS.
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xiu-Fang
authors.identifier: 0000-0001-5513-6202
authors.initials: XF
authors.lastname: Ding
authors.affiliation: Henan University of Chinese Medicine, Henan, China; School of Pre-Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Zhi-Yi
authors.initials: ZY
authors.lastname: Yan
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Ma
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Zhong-Ye
authors.initials: ZY
authors.lastname: Jin
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yue-Yun
authors.initials: YY
authors.lastname: Liu
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Xue
authors.affiliation: School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Jia-Xu
authors.identifier: 0000-0002-5570-6233
authors.initials: JX
authors.lastname: Chen
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Zhi-Ping
authors.identifier: 0000-0003-2796-526X
authors.initials: ZP
authors.lastname: Lv