title: Medicago truncatula ABCG10 is a transporter of 4-coumarate and liquiritigenin in the medicarpin biosynthetic pathway.
abstract: The ABCG10 protein of the model legume Medicago truncatula is required for efficient de novo production of the phenylpropanoid-derived phytoalexin medicarpin. Silencing the expression of MtABCG10 results, inter alia, in a lower accumulation of medicarpin and its precursors. In this study, we demonstrate that the impairment of medicarpin biosynthesis can be partially averted by the exogenous application of 4-coumarate, an early precursor of the core phenylpropanoid pathway, and the deoxyisoflavonoid formononetin. Experiments conducted using HPLC/MS in a heterologous system as well as in vitro transport assays with labelled substrate revealed that MtABCG10 is responsible for the membrane translocation of 4-coumarate and liquiritigenin, molecules representing key branching points in the phenylpropanoid pathway. The identification of transporters participating in the distribution of precursors is an important step in understanding phenylpropanoid biosynthesis.
authors.affiliation: Department of Natural Products Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.|Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland.
authors.forename: Wanda
authors.initials: W
authors.lastname: Biala
authors.affiliation: Department of Natural Products Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences,Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Banasiak
authors.affiliation: Department of Natural Products Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.|Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Jarzyniak
authors.affiliation: Department of Natural Products Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences,Poznan, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Pawela
authors.affiliation: Department of Natural Products Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.|Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Jasinski
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D003373:Coumaric Acids; D044950:Flavanones / Q000378:metabolism*; D046913:Medicago truncatula / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D036343:Pterocarpans / Q000378:metabolism; D000094162:Phytoalexins
chemical_list: D000070996:ATP Binding Cassette Transporter, Subfamily G; D003373:Coumaric Acids; D044950:Flavanones; D010940:Plant Proteins; D011422:Propionates; D036343:Pterocarpans; C047353:medicarpin; D000094162:Phytoalexins; C495469:p-coumaric acid; C083152:liquiritigenin
keywords: 4-coumarate; ABC transporters; Medicago truncatula; biotic stress; liquiritigenin; medicarpin; phenylpropanoid pathway