title: Frequency of Epstein Barr Virus Type 1 Among Nasopharyngeal Carcinomas in Iranian Patients
abstract: Background: Around 95% of the world’s population are infected with the Epstein-Barr virus (EBV), which
  can persist latent in B lymphocytes and epithelial cells life-long. EBV has been linked with lymphoid and epithelial
  cancers and persistence of EBV infection in lymphoid or epithelial cells may result in virus-associated B-cell
  tumors or nasopharyngeal carcinomas (NPC). This study was conducted to determine the frequency of EBV DNA in
  nasopharyngeal carcinoma tissue of Iranian patients. Materials and methods: A total of 50 blocks of formalin-fixed
  paraffin-embedded tissue of NPCs from 38 (76 %) male and 12 (24%) female patients were collected from archives
  of Ahvaz hospitals. Sections were cut at 5 μm and DNA was extracted for detection of EBV DNA and EBV typing by
  mested PCR. DNA sequencing was performed to confirm PCR results. The distribution of EBV DNA was compared
  among WHO histological subtypes of NPC. Results: Some 3 female and 11 (22%) male NPC samples showed positive
  for EBV DNA type 1, 2/14(22.2%)WHO histological type II and 12/41(29.3%) WHO histological type III. Conclusions:
  The frequency of EBV DNA among NPCs in Iranian patients was found to be 28%, EBV type I predominating. Both
  WHO histological type II and III NPC subtypes demonstrated approximately the same detection prevalence.
authors.affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Email:manoochehrmakvandi29@yahoo.com
authors.forename: Toran
authors.initials: T
authors.lastname: Shahani
authors.forename: Manoochehr
authors.initials: M
authors.lastname: Makvandi
authors.forename: Alireza
authors.initials: A
authors.lastname: Samarbafzadeh
authors.forename: Ali
authors.initials: A
authors.lastname: Teimoori
authors.forename: Nastran
authors.initials: N
authors.lastname: Ranjbar
authors.forename: Nader
authors.initials: N
authors.lastname: saki
authors.forename: Soheila
authors.initials: S
authors.lastname: Nikakhlagh
authors.forename: Niloofar
authors.initials: N
authors.lastname: Neisi
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Hosseini
authors.forename: Samira
authors.initials: S
authors.lastname: Pourrezaei
authors.forename: Abdolnabi
authors.initials: A
authors.lastname: Shabani
authors.forename: Hashem
authors.initials: H
authors.lastname: Radmehr
authors.forename: Hamide
authors.initials: H
authors.lastname: Mehravaran
authors.forename: Hadis
authors.initials: H
authors.lastname: Kiani
authors.forename: Azadeh
authors.initials: A
authors.lastname: Haghi
keywords: Epstein-Bar (EBV); nasopharyngeal carcinoma (NCP); nested PCR