title: Nitric oxide inhibits aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips.
abstract: It had been reported that Aluminum (Al) stress altered nitric oxide (NO) concentration and induced programmed cell death (PCD) in plants. However, the relationship between NO and PCD occurrence under Al stress is unclear. The results showed that cell death induced by Al was significant negative correlation with the inhibition of Al on root elongation growth in peanut. AlCl3at 100μmolL-1induced DNA ladder, chromatin condensation, typical apoptotic chromatin condensation staining with DAPI, apoptosis related gene Hrs203j expression and caspase3-like protease activation in peanut root tip cells, and showed that Al-induced cell death in peanut root tip cells was a typical PCD. Exogenous NO donor sodium nitroprusside (SNP) at 200μmolL-1inhibited Al-induced PCD occurrence, but NO specific scavenger cPTIO aggravated PCD production. It suggests that NO is a negative regulator of Al-induced PCD in peanut root tips.
authors.affiliation: College of Agronomy, Guangxi University, Nanning 530004, PR China; Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, PR China.
authors.forename: Huyi
authors.initials: H
authors.lastname: He
authors.affiliation: College of Agronomy, Guangxi University, Nanning 530004, PR China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Agronomy, Guangxi University, Nanning 530004, PR China.
authors.forename: Thet Lwin
authors.initials: TL
authors.lastname: Oo
authors.affiliation: College of Agronomy, Guangxi University, Nanning 530004, PR China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Gu
authors.affiliation: College of Agronomy, Guangxi University, Nanning 530004, PR China; Guangxi Colleges and Universities Key Laboratory of Crop Cultivation and Tillage, Nanning 530004, PR China. Electronic address: lfhe@gxu.edu.cn.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: He
mesh_terms: D000222:Adaptation, Physiological; D000535:Aluminum / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D010367:Arachis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004789:Enzyme Activation; D017343:Genes, Plant; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development
chemical_list: D002843:Chromatin; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D000535:Aluminum; D053148:Caspase 3
keywords: Al toxicity; Nitric oxide; Peanut; Programmed cell death; Root tips