title: Deproteinization potential and antioxidant property of haloalkalophilic organic solvent tolerant protease from marineBacillussp. APCMST-RS3 using marine shell wastes.
abstract: The current increase in the vast amount of marine crustacean shell waste produced by the fish processing industries has led to the need to find new methods for its disposal. Hence, the present study was carried out via marine shell wastes as substrate for protease production. The maximum production (4000.65 U/ml) fromBacillussp. APCMST-RS3 was noticed in 3:1% shrimp and oyster shell powder (SOSP) as substrate. Purified protease showed 53.22% and 22.66% enzyme yield; 3.48 and 8.49 fold purity with 40 kDa molecular weight; whereas, itsKmandVmaxvalues were 0.6666 g/l, 1111.11 U/ml. This enzyme showed optimum activity at pH 9 and 60 °C temperature. Also, it retained maximum protease activity in the presence of NaCl (2.5 M), surfactants (Tween 20, 40, 60, 80 and SDS) and metal ions (MnCl2, CaCl2, HgCl2and BaCl2) and solvents. The candidate bacterium effectively deproteinized (84.35%) shrimp shell and its antioxidant potentials.
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamil nadu, India.
authors.forename: Thirumalai
authors.initials: T
authors.lastname: Maruthiah
authors.affiliation: Department of Zoology, Rani Anna Government College for Women, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil nadu, India.
authors.forename: Beena
authors.initials: B
authors.lastname: Somanath
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamil nadu, India.
authors.forename: Grasian
authors.initials: G
authors.lastname: Immanuel
authors.affiliation: Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil nadu, India.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Palavesam
keywords: Antioxidant activity; Crustacean wastes; Deproteinization; Marine alkaline protease; Solvent tolerance