title: Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation.
abstract: Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).
authors.affiliation: Division of Biomedical Engineering , The Chinese University of Hong Kong , Hong Kong , China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, Institute of Molecular Functional Materials , The Hong Kong University of Science and Technology (HKUST) , Kowloon, Hong Kong , China.
authors.forename: Chris Wai Tung
authors.initials: CWT
authors.lastname: Leung
authors.affiliation: Division of Biomedical Engineering , The Chinese University of Hong Kong , Hong Kong , China.
authors.forename: Dexter Siu Hong
authors.initials: DSH
authors.lastname: Wong
authors.affiliation: Division of Biomedical Engineering , The Chinese University of Hong Kong , Hong Kong , China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Division of Biomedical Engineering , The Chinese University of Hong Kong , Hong Kong , China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Chemistry, Institute of Molecular Functional Materials , The Hong Kong University of Science and Technology (HKUST) , Kowloon, Hong Kong , China.
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Institute of Molecular Functional Materials , The Hong Kong University of Science and Technology (HKUST) , Kowloon, Hong Kong , China.
authors.forename: Chris Yu Yee
authors.initials: CYY
authors.lastname: Yung
authors.affiliation: Department of Chemistry, Institute of Molecular Functional Materials , The Hong Kong University of Science and Technology (HKUST) , Kowloon, Hong Kong , China.
authors.forename: Engui
authors.initials: E
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Institute of Molecular Functional Materials , The Hong Kong University of Science and Technology (HKUST) , Kowloon, Hong Kong , China.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.affiliation: Division of Biomedical Engineering , The Chinese University of Hong Kong , Hong Kong , China.|China Orthopedic Regenerative Medicine Group (CORMed) , Hangzhou , China.
authors.forename: Liming
authors.identifier: http://orcid.org/0000-0003-4739-0918
authors.initials: L
authors.lastname: Bian
mesh_terms: D015415:Biomarkers / Q000097:blood; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D017319:Photosensitizing Agents / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D012822:Silicon Dioxide / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D015019:Yttrium / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D017319:Photosensitizing Agents; D034741:RNA, Small Interfering; D015019:Yttrium; D012822:Silicon Dioxide; D053509:Matrix Metalloproteinase 13
keywords: aggregation-induced emission; controlled differentiation; real-time monitoring cell differentiation; stem cell; up-conversion nanoparticles