title: Immunological implications of bovine leukemia virus infection.
abstract: This study examined neutrophil and monocyte functions and the blood lymphocyte profile of naturally BLV-infected cows with or without persistent lymphocytosis (PL). The percentage of neutrophils and monocytes that phagocytosed Staphylococcus aureus was lower in BLV-infected dairy cows, particularly those with PL. The relative percentage of CD44+monocytes and neutrophils and CD11b expression by neutrophils was also lower in BLV-infected dairy cows with PL. A correlation between the percentage of CD11b+neutrophils and that produced reactive oxygen species (ROS) was found. Furthermore, the percentage of CD44+monocytes was positively correlated with the percentage of monocytes that phagocytosed S. aureus and the same phenomenon was observed for neutrophils. In BLV-infected dairy cows, particularly those with PL, inhibition of monocyte and neutrophil apoptosis was observed. Additionally, the percentage of neutrophils producing ROS was lower in BLV-infected cows with PL, in contrast to higher intensity of intracellular production of ROS by monocytes. The result from the lymphocyte immunophenotyping of BLV-infected cows with PL was an increase in B cells, mainly B CD5+CD11b+, due to the apoptosis inhibition. In conclusion, this study provides novel insight into the implications of BLV infection for cattle, which can include the dysfunction of blood monocytes and neutrophils.
authors.affiliation: Veterinary Clinical Immunology Research Group, Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Blagitz
authors.affiliation: Veterinary Clinical Immunology Research Group, Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil. Electronic address: nogueirasouza@usp.br.
authors.forename: F N
authors.initials: FN
authors.lastname: Souza
authors.affiliation: Veterinary Clinical Immunology Research Group, Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Batista
authors.affiliation: Veterinary Clinical Immunology Research Group, Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
authors.forename: L F F
authors.initials: LFF
authors.lastname: Azevedo
authors.affiliation: Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas Carvalho de Aguiar, 470, São Paulo 05403-000, Brazil.
authors.forename: E M R
authors.initials: EMR
authors.lastname: Sanchez
authors.affiliation: Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais, Av. Presidente Antônio Carlos, 6627, Belo Horizonte 31270-010, Brazil.
authors.forename: S A
authors.initials: SA
authors.lastname: Diniz
authors.affiliation: Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais, Av. Presidente Antônio Carlos, 6627, Belo Horizonte 31270-010, Brazil.
authors.forename: M X
authors.initials: MX
authors.lastname: Silva
authors.affiliation: Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais, Av. Presidente Antônio Carlos, 6627, Belo Horizonte 31270-010, Brazil.
authors.forename: J P
authors.initials: JP
authors.lastname: Haddad
authors.affiliation: Veterinary Clinical Immunology Research Group, Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
authors.forename: A M M P
authors.initials: AMMP
authors.lastname: Della Libera
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000276:immunology / Q000821:virology*; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D016130:Immunophenotyping; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008218:Lymphocytosis; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D039481:CD11b Antigen; D018960:Hyaluronan Receptors
keywords: Deltaretrovirus; Enzootic bovine leukosis; Immune response; Persistent lymphocytosis