title: Bioactivities of fish protein hydrolysates from defatted salmon backbones.
abstract: Bioactivities of bulk fish protein hydrolysates (FPH) from defatted salmon backbones obtained with eight different commercial enzymes and their combinations were tested. All FPH showed antioxidative activityin vitro. DPPH scavenging activity increased, while iron chelating ability decreased with increasing time of hydrolysis. All FPH showed ACE inhibiting effect which depended on type of enzyme and increased with time of hydrolysis. The highest effect was found for FPH produced with Trypsin. Bromelain + Papain hydrolysates reduced the uptake of radiolabelled glucose into CaCo-2 cells, a model of human enterocytes, indicating a potential antidiabetic effect of FPH. FPH obtained by Trypsin, Bromelain + Papain and Protamex showed the highest ACE inhibitory, cellular glucose transporter (GLUT/SGLT) inhibitory andin vitroantioxidative activities, respectively. Correlation was observed between the measured bioactivities, degree of hydrolysis and molecular weight profiles, supporting prolonged hydrolysis to obtain high bioactivities.
authors.affiliation: SINTEF Fisheries and Aquaculture, Norway.
authors.forename: Rasa
authors.initials: R
authors.lastname: Slizyte
authors.affiliation: VTT Technical Research Centre of Finland Ltd, Finland.
authors.forename: Katariina
authors.initials: K
authors.lastname: Rommi
authors.affiliation: SINTEF Fisheries and Aquaculture, Norway.
authors.forename: Revilija
authors.initials: R
authors.lastname: Mozuraityte
authors.affiliation: Department of Human Nutritional Sciences, University of Manitoba, Canada.
authors.forename: Peter
authors.initials: P
authors.lastname: Eck
authors.affiliation: Norwegian University of Science and Technology, Norway.
authors.forename: Kathrine
authors.initials: K
authors.lastname: Five
authors.affiliation: Norwegian University of Science and Technology, Norway.
authors.forename: Turid
authors.initials: T
authors.lastname: Rustad
keywords: ACE-inhibition; Antioxidant; Fish protein hydrolysates; Glucose transport inhibition; Salmon backbones