title: Clerodane Diterpenoid Glucosides from the Stems of Tinospora sinensis.
abstract: Ten new clerodane diterpenoid glucosides (1-10) and three known analogues (11-13) were isolated from an EtOAc extract of the stems of Tinospora sinensis. Spectroscopic analyses and chemical methods were used to elucidate the structures of these isolates. The absolute configurations of tinosinenosides A-C (1-3) were established by using experimental and calculated ECD data. Their cytotoxicity against the human epithelioid cervical carcinoma (HeLa) cell line and the nitric oxide production inhibitory activity of lipopolysaccharide-activated N9 microglial cells were tested. 1-Deacetyltinosposide A (12) exhibited mild cytotoxicity against HeLa cells, with an IC50value of 8.35 ± 0.60 μM.
authors.affiliation: State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University , Guilin 541004, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Pharmacy, Guilin Medical University , Guilin 541004, People's Republic of China.
authors.forename: Gui-Jie
authors.initials: GJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, People's Republic of China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University , Guilin 541004, People's Republic of China.
authors.forename: Heng-Shan
authors.initials: HS
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University , Guilin 541004, People's Republic of China.
authors.forename: Dong
authors.identifier: 0000-0002-9765-7548
authors.initials: D
authors.lastname: Liang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D018547:Plant Stems / Q000737:chemistry*; D031610:Tinospora / Q000737:chemistry*
chemical_list: C547240:1-deacetyltinosposide A; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D045785:Diterpenes, Clerodane; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D008070:Lipopolysaccharides; C000621333:tinosinenoside A; C000621334:tinosinenoside B; C000621336:tinosinenoside C; D009569:Nitric Oxide