title: Long-term blood glucose monitoring with implanted telemetry device in conscious and stress-free cynomolgus monkeys.
abstract: OBJECTIVE
  Continuous blood glucose monitoring, especially long-term and remote, in diabetic patients or research is very challenging. Nonhuman primate (NHP) is an excellent model for metabolic research, because NHPs can naturally develop Type 2 diabetes mellitus (T2DM) similarly to humans. This study was to investigate blood glucose changes in conscious, moving-free cynomolgus monkeys (Macaca fascicularis) during circadian, meal, stress and drug exposure.
  
  
  METHODS
  Blood glucose, body temperature and physical activities were continuously and simultaneously recorded by implanted HD-XG telemetry device for up to 10 weeks.
  
  
  CONCLUSIONS
  Blood glucose circadian changes in normoglycemic monkeys significantly differed from that in diabetic animals. Postprandial glucose increase was more obvious after afternoon feeding. Moving a monkey from its housing cage to monkey chair increased blood glucose by 30% in both normoglycemic and diabetic monkeys. Such increase in blood glucose declined to the pre-procedure level in 30 min in normoglycemic animals and >2 h in diabetic monkeys. Oral gavage procedure alone caused hyperglycemia in both normoglycemic and diabetic monkeys. Intravenous injection with the stress hormones, angiotensin II (2 μg/kg) or norepinephrine (0.4 μg/kg), also increased blood glucose level by 30%. The glucose levels measured by the telemetry system correlated significantly well with glucometer readings during glucose tolerance tests (ivGTT or oGTT), insulin tolerance test (ITT), graded glucose infusion (GGI) and clamp.
  
  
  CONCLUSIONS
  Our data demonstrate that the real-time telemetry method is reliable for monitoring blood glucose remotely and continuously in conscious, stress-free, and moving-free NHPs with the advantages highly valuable to diabetes research and drug discovery.
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: G
authors.initials: G
authors.lastname: Sun
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Ye
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Data Sciences International, St. Paul, MN, USA.
authors.forename: R
authors.initials: R
authors.lastname: Lindquist
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Crown Bioscience, Inc., Taicang, Jiangsu Province, The People's Republic of China. xiaoyongfu@crownbio.com.
authors.forename: Y-F
authors.initials: YF
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001831:Body Temperature / Q000502:physiology; D003243:Consciousness / Q000502:physiology*; D005260:Female; D005951:Glucose Tolerance Test / Q000295:instrumentation / Q000379:methods / Q000639:trends; D008124:Locomotion / Q000502:physiology*; D008252:Macaca fascicularis; D008297:Male; D013686:Telemetry / Q000295:instrumentation / Q000379:methods / Q000639:trends*; D013997:Time Factors
chemical_list: D001786:Blood Glucose
keywords: Continuous glucose monitoring; Diabetes; Glucose circadian; Implantable telemetry device; Nonhuman primate