title: The effect of cannabinoid receptor 1 blockade on hepatic free fatty acid profile in mice with nonalcoholic fatty liver disease.
abstract: We used rimonabant to investigate the role of CB1 receptor on hepatic FFAs profile during NAFLD. Male mice C57BL/6 were divided into: control group fed with control diet 20 weeks (C; n=6); group fed with HFD 20 weeks (HF; n=6); group fed with control diet and treated with rimonabant after 18 weeks (R; n=9); group fed with HFD and treated with rimonabant after 18 weeks (HFR; n=10). Rimonabant (10mg/kg) was administered daily to HFR and R group by oral gavage. Rimonabant decreased liver palmitic acid proportion in HFR group compared to HF group (p<0.05). Liver stearic and oleic acid proportions were decreased in R group compared to control (p<0.01 respectively). Rimonabant increased liver linoleic and arachidonic acid proportions in HFR group compared to HF group (p<0.01 respectively). CB1 blockade may be useful in the treatment of HFD-induced NAFLD due to modulation of plasma lipid and hepatic FFA profile.
authors.affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Serbia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Jorgačević
authors.affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Vučević
authors.affiliation: Department for Bromatology, Faculty of Farmacy, University of Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Đuričić
authors.affiliation: Department for Bromatology, Faculty of Farmacy, University of Belgrade, Serbia.
authors.forename: Slađana
authors.initials: S
authors.lastname: Šobajić
authors.affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Serbia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Mladenović
authors.affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Vesković
authors.affiliation: Institute of Digestive Diseases, Clinical Centre of Serbia, Belgrade, Serbia.
authors.forename: Rada Ješić
authors.initials: RJ
authors.lastname: Vukićević
authors.affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Serbia. Electronic address: tanjamm@med.bg.ac.rs.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Radosavljević
mesh_terms: D000284:Administration, Oral; D000818:Animals; D063387:Cannabinoid Receptor Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D059305:Diet, High-Fat; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology*; D011720:Pyrazoles / Q000008:administration & dosage / Q000494:pharmacology*; D043885:Receptor, Cannabinoid, CB2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000077285:Rimonabant
chemical_list: D063387:Cannabinoid Receptor Antagonists; C571431:Cnr2 protein, mouse; D005230:Fatty Acids, Nonesterified; D010880:Piperidines; D011720:Pyrazoles; D043885:Receptor, Cannabinoid, CB2; D000077285:Rimonabant
keywords: CB1 receptor blockade; Endocannabinoid system; Free fatty acid profile; Mice; NAFLD; Rimonabant