title: A continuous system for biocatalytic hydrogenation of CO2to formate.
abstract: In this work a novel bioprocess for hydrogenation of CO2to formate was developed, using whole cell catalysis by a sulfate-reducing bacterium. Three Desulfovibrio species were tested (D. vulgaris Hildenborough, D. alaskensis G20, and D. desulfuricans ATCC 27774), of which D. desulfuricans showed the highest activity, producing 12mM of formate in batch, with a production rate of 0.09mMh-1. Gene expression analysis indicated that among the three formate dehydrogenases and five hydrogenases, the cytoplasmic FdhAB and the periplasmic [FeFe] HydAB are the main enzymes expressed in D. desulfuricans in these conditions. The new bioprocess for continuous formate production by D. desulfuricans had a maximum specific formate production rate of 14mMgdcw-1h-1, and more than 45mM of formate were obtained with a production rate of 0.40mMh-1. This is the first report of a continuous process for biocatalytic formate production.
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier/Universidade Nova de Lisboa (ITQB NOVA), Av. da Republica-EAN, 2780-157 Oeiras, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Mourato
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier/Universidade Nova de Lisboa (ITQB NOVA), Av. da Republica-EAN, 2780-157 Oeiras, Portugal.
authors.forename: Mónica
authors.initials: M
authors.lastname: Martins
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier/Universidade Nova de Lisboa (ITQB NOVA), Av. da Republica-EAN, 2780-157 Oeiras, Portugal.
authors.forename: Sofia M
authors.initials: SM
authors.lastname: da Silva
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier/Universidade Nova de Lisboa (ITQB NOVA), Av. da Republica-EAN, 2780-157 Oeiras, Portugal. Electronic address: ipereira@itqb.unl.pt.
authors.forename: Inês A C
authors.initials: IAC
authors.lastname: Pereira
mesh_terms: D055162:Biocatalysis; D002245:Carbon Dioxide / Q000378:metabolism*; D003901:Desulfovibrio / Q000378:metabolism; D005560:Formate Dehydrogenases / Q000378:metabolism*; D005561:Formates / Q000378:metabolism*; D006865:Hydrogenation
chemical_list: D005561:Formates; D002245:Carbon Dioxide; D005560:Formate Dehydrogenases
keywords: Formate production; H(2) storage; Hydrogenation of CO(2); Sulfate reducing bacteria; Whole cell biocatalysis