title: MiDAS 2.0: an ecosystem-specific taxonomy and online database for the organisms of wastewater treatment systems expanded for anaerobic digester groups.
abstract: Wastewater is increasingly viewed as a resource, with anaerobic digester technology being routinely implemented for biogas production. Characterising the microbial communities involved in wastewater treatment facilities and their anaerobic digesters is considered key to their optimal design and operation. Amplicon sequencing of the 16S rRNA gene allows high-throughput monitoring of these systems. The MiDAS field guide is a public resource providing amplicon sequencing protocols and an ecosystem-specific taxonomic database optimized for use with wastewater treatment facility samples. The curated taxonomy endeavours to provide a genus-level-classification for abundant phylotypes and the online field guide links this identity to published information regarding their ecology, function and distribution. This article describes the expansion of the database resources to cover the organisms of the anaerobic digester systems fed primary sludge and surplus activated sludge. The updated database includes descriptions of the abundant genus-level-taxa in influent wastewater, activated sludge and anaerobic digesters. Abundance information is also included to allow assessment of the role of emigration in the ecology of each phylotype. MiDAS is intended as a collaborative resource for the progression of research into the ecology of wastewater treatment, by providing a public repository for knowledge that is accessible to all interested in these biotechnologically important systems.
  
  
  
  http://www.midasfieldguide.org.
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Simon Jon
authors.initials: SJ
authors.lastname: McIlroy
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Rasmus Hansen
authors.initials: RH
authors.lastname: Kirkegaard
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Bianca
authors.initials: B
authors.lastname: McIlroy
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Marta
authors.initials: M
authors.lastname: Nierychlo
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Jannie Munk
authors.initials: JM
authors.lastname: Kristensen
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Søren Michael
authors.initials: SM
authors.lastname: Karst
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Mads
authors.initials: M
authors.lastname: Albertsen
authors.affiliation: Department of Chemistry and Bioscience, Center for Microbial Communities, Aalborg University, Aalborg DK-9220, Denmark.
authors.forename: Per Halkjær
authors.initials: PH
authors.lastname: Nielsen
mesh_terms: D000693:Anaerobiosis; D002965:Classification; D017753:Ecosystem; D020459:Genes, rRNA / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology; D018508:Water Purification
chemical_list: D012336:RNA, Ribosomal, 16S; D062065:Wastewater