title: Determination of Vancomycin in Human Serum by Cyclodextrin-Micellar Electrokinetic Capillary Chromatography (CD-MEKC) and Application for PDAP Patients.
abstract: A simple and sensitive cyclodextrin-micellar electrokinetic capillary chromatography (CD-MEKC) method with UV detection was developed and validated for the determination of vancomycin (VCM) in serum. The separation was achieved in 14 min at 25 °C with a fused-silica capillary column of 40.2 cm × 50 mm i.d. (effective length 30.2 cm) and a run buffer containing 25 mM borate buffer with 50 mM sodium dodecylsulfonate (SDS) (pH 9.5) and 2% sulfobutyl-β-cyclodextrin (sulfobutyl-β-CD). Under optimal conditions for biological samples, good separations with high efficiency and short analysis time were achieved. Several parameters affecting the drug separation from biological matrices were studied, including buffer types, concentrations, and pHs. The methods were validated over the range of 0.9998-99.98 µg/mL. Calibration curves of VCM also showed good linearity (r² > 0.999). Intra- and interday precisions (relative standard deviation, RSD) were less than 5.80% and 7.38%, and lower limit of quantification (LLOQ) were lower than 1.0 μg/mL. The mean recoveries ranged between 84.03% and 91.69%. The method was successfully applied for monitoring VCM concentrations in serum of patients with peritoneal dialysis-associated peritonitis (PDAP). The assay should be applicable to pharmacokinetic studies and routine therapeutic drug monitoring of this drug in serum.
authors.affiliation: Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, No. 100 Haining Road, Shanghai 200080, China. wangjiajing77@163.com.
authors.forename: Jiajing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. caoyuqing1109@163.com.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Laboratory of Drug Metabolism & Pharmacokinetics, School of Medicine, Tongji University, No. 1239 Siping Road, Shanghai 200092, China. wu-shengyuan@tongji.edu.cn.
authors.forename: Shengyuan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, No. 100 Haining Road, Shanghai 200080, China. wangshuowensy@163.com.
authors.forename: Shuowen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, No. 24 Tong Jia Xiang, Nanjing 210009, China. yoyo0132@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. tingting_zoo@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, Branch of Shanghai First People's Hospital, Shanghai 200081, China. fanguorong@sjtu.edu.cn.
authors.forename: Yuefen
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, No. 100 Haining Road, Shanghai 200080, China. louyuefen@sina.cn.|Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. louyuefen@sina.cn.|Laboratory of Drug Metabolism & Pharmacokinetics, School of Medicine, Tongji University, No. 1239 Siping Road, Shanghai 200092, China. louyuefen@sina.cn.
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D016903:Drug Monitoring; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010530:Peritoneal Dialysis / Q000009:adverse effects; D010538:Peritonitis / Q000188:drug therapy* / Q000382:microbiology; D014640:Vancomycin / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D003505:Cyclodextrins; D014640:Vancomycin
keywords: cyclodextrin-micellar electrokinetic capillary chromatography; peritoneal dialysis-associated peritonitis; vancomycin