title: Human Rhinovirus-A1 as an Expression Vector.
abstract: Expression vectors that are based on live human rhinoviruses (HRVs) are attractive, yet often overlooked in vaccine development due to their limited capacity for foreign gene inserts and poor genetic stability. This chapter describes a novel methodology to engineer a replication-competent genetically stable recombinant HRV (rHRV) without affecting viral replication capability. We have previously used these methods to generate live, genetically stable recombinant HRVs encoding HIV Gag and Tat proteins (rHRV-Gag-Tat), a potential mucosally targeted HIV vaccine.
authors.affiliation: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia. khamis.tomusange@adelaide.edu.au.
authors.forename: Khamis
authors.initials: K
authors.lastname: Tomusange
authors.affiliation: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia.
authors.forename: Danushka
authors.initials: D
authors.lastname: Wijesundara
authors.affiliation: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia.
authors.forename: Eric James
authors.initials: EJ
authors.lastname: Gowans
authors.affiliation: Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia.
authors.forename: Branka
authors.initials: B
authors.lastname: Grubor-Bauk
mesh_terms: D004770:Enterovirus / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000276:immunology; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000276:immunology
chemical_list: D054301:gag Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
keywords: HIV-Gag and HIV-Tat; HRV-A1; Live vaccine vector