title: Anti-inflammatory effects of essential oils from Mangifera indica.
abstract: Mangifera indica is widely found in Brazil, and its leaves are used as an anti-inflammatory agent in folk medicine. The aim of this study is to perform composition analysis of essential oils from the M. indica varieties, espada (EOMIL1) and coração de boi (EOMIL2), and confirm their anti-inflammatory properties. Twenty-three volatile compounds were identified via gas chromatography-mass spectrometry (GC-MS) in two essential oils from the leaves. Paw edema and myeloperoxidase (MPO) activity were evaluated using the carrageenan-induced paw model, while leukocyte migration was analyzed using the pleurisy model. At oral doses of 100 and 300 mg/kg, the essential oils significantly reduced edema formation and the increase in MPO activity induced by carrageenan in rat paws. For a dose of 300 mg/kg EOMIL1, 62 ± 8% inhibition of edema was observed, while EOMIL2 led to 51 ± 7% inhibition of edema. At a dose of 100 mg/kg, the inhibition was 54 ± 9% for EOMIL1 and 37 ± 7% for EOMIL2. EOMIL1 and EOMIL2 significantly reduced MPO activity at doses of 100 mg/kg (47 ± 5 and 23 ± 8%, respectively) and 300 mg/kg (50 ± 9 and 31 ± 7%, respectively). In the pleurisy model, inhibitions were also observed for EOMIL1 and EOMIL2 in the leukocyte migration test. The results of the present study show that essential oils from M. indica differ in chemical composition and anti-inflammatory activity in rats.
authors.affiliation: Laboratório de Química Analítica, Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: R M
authors.initials: RM
authors.lastname: Oliveira
authors.affiliation: Laboratório de Química Analítica, Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: T S
authors.initials: TS
authors.lastname: Dutra
authors.affiliation: Laboratório de Produtos Naturais, Curso de Química, Universidade Estadual de Mato Grosso do Sul, Navirai, MS, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Simionatto
authors.affiliation: Laboratório de Química Analítica, Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: N
authors.initials: N
authors.lastname: Ré
authors.affiliation: Laboratório de Imunoinflamação e Dor, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, MS, Brasil.
authors.forename: C A L
authors.initials: CA
authors.lastname: Kassuya
authors.affiliation: Laboratório de Análise Instrumental, Centro de Estudos em Recursos Naturais, Universidade Estadual de Mato Grosso do Sul, Dourados, MS, Brasil claudia@uems.br.
authors.forename: C A L
authors.initials: CA
authors.lastname: Cardoso
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D001938:Brazil; D002351:Carrageenan / Q000009:adverse effects; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005786:Gene Expression Regulation / Q000187:drug effects; D007962:Leukocytes / Q000145:classification / Q000187:drug effects; D008297:Male; D031022:Mangifera / Q000737:chemistry*; D008519:Medicine, Traditional; D051379:Mice; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010938:Plant Oils; D002351:Carrageenan; D009195:Peroxidase