title: Sulforaphene enhances radiosensitivity of hepatocellular carcinoma through suppression of the NF-κB pathway.
abstract: Sulforaphene (SFE), a naturally occurring isothiocyanate found in cruciferous vegetables, has attracted increasing attention for its anti-cancer effect in many cancers, including hepatocellular carcinoma (HCC). However, the precise role of SFE in the radiosensitivity of HCC is still unclear. Here, cell proliferation and apoptosis were detected by MTT and flow cytometry assay, respectively. The activity of NF-κB was further evaluated by ELISA. We also observed the effect of SFE and/or radiation on tumor growth. The results showed that SFE inhibited cell proliferation and induced apoptosis in HCC cells. Radiation increased NF-kB activity, while PDTC, a NF-kB inhibitor, enhanced radiation-induced cell death. SFE inhibited NF-kB activity and the downstream gene expressions of the NF-kB pathway in HCC cells. Moreover, SFE enhanced the inhibitory effect of radiation on tumor growth both in vitro and in vivo. This study indicated that SFE sensitized the radiosensitivity of HCC by blocking the NF-kB pathway.
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Institute of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Treatment and Clinical Research Center of Henan Province, Zhengzhou, 450052, People's Republic of China.
authors.forename: Kewei
authors.initials: K
authors.lastname: Ren
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Institute of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Treatment and Clinical Research Center of Henan Province, Zhengzhou, 450052, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Institute of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Treatment and Clinical Research Center of Henan Province, Zhengzhou, 450052, People's Republic of China.
authors.forename: Yahua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Institute of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Treatment and Clinical Research Center of Henan Province, Zhengzhou, 450052, People's Republic of China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Institute of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.|Interventional Treatment and Clinical Research Center of Henan Province, Zhengzhou, 450052, People's Republic of China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D056945:Hep G2 Cells; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016328:NF-kappa B / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011836:Radiation Tolerance / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D013454:Sulfoxides; D023041:Xenograft Model Antitumor Assays
chemical_list: D017879:Isothiocyanates; D016328:NF-kappa B; D009363:Neoplasm Proteins; D013454:Sulfoxides; C016766:sulforaphane
keywords: NF-κB pathway; hepatocellular carcinoma; radiosensitivity; sulforaphene