title: Biosynthesis of α-Glucosidase Inhibitors by a Newly Isolated Bacterium, Paenibacillus sp. TKU042 and Its Effect on Reducing Plasma Glucose in a Mouse Model.
abstract: Paenibacillus sp. TKU042, a bacterium isolated from Taiwanese soil, produced α-glucosidase inhibitors (aGIs) in the culture supernatant when commercial nutrient broth (NB) was used as the medium for fermentation. The supernatant of fermented NB (FNB) showed stronger inhibitory activities than acarbose, a commercial anti-diabetic drug. The IC50 and maximum α-glucosidase inhibitory activities (aGIA) of FNB and acarbose against α-glucosidase were 81 μg/mL, 92% and 1395 μg/mL, 63%, respectively. FNB was found to be strongly thermostable, retaining 95% of its relative activity, even after heating at 100 °C for 30 min. FNB was also stable at various pH values. Furthermore, FNB demonstrated antioxidant activity (IC50 = 2.23 mg/mL). In animal tests, FNB showed remarkable reductions in the plasma glucose of ICR (Institute of Cancer Research) mice at a concentration of 200 mg/kg. Combining FNB and acarbose enhanced the effect even more, with an added advantage of eliminating diarrhea. According to HPLC (High-performance liquid chromatography) fingerprinting, the Paenibacillus sp. TKU042 aGIs were not acarbose. All of the results suggest that Paenibacillus sp. TKU042 FNB could have potential use as a health food or to treat type 2 diabetes.
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. bondhtn@gmail.com.|Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam. bondhtn@gmail.com.
authors.forename: Van Bon
authors.initials: VB
authors.lastname: Nguyen
authors.affiliation: Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam. nadzungtaynguyenuni@yahoo.com.vn.
authors.forename: Anh Dzung
authors.initials: AD
authors.lastname: Nguyen
authors.affiliation: Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan. kuoyh@nricm.edu.tw.|Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. kuoyh@nricm.edu.tw.
authors.forename: Yao-Haur
authors.initials: YH
authors.lastname: Kuo
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. sabulo@mail.tku.edu.tw.
authors.forename: San-Lang
authors.initials: SL
authors.lastname: Wang
mesh_terms: D020909:Acarbose / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D056507:Paenibacillus / Q000737:chemistry* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D000520:alpha-Glucosidases; D020909:Acarbose
keywords: Paenibacillus; acarbose; plasma glucose; type 2 diabetes; α-glucosidase inhibitor