title: Residual determination of anesthetic menthol in fishes by SDME/GC-MS.
abstract: The SDME-GC/MS method was applied to residual determination of anesthetic menthol in fish. The extractions took place from the headspace of the sample using 1.8µL of octane as the extraction solvent. To obtain the ideal extraction condition, was used Response Surface Methodology, defining: extraction time 15min, temperature 30°C and salt 3g. The method showed LOD and LOQ of 0.021 and 1.56µgL-1respectively, recovery of 94% and R2of 0.9997. The analyzes were performed on tilapia fillets anesthetized in five concentrations between 5 and 15×104µgL-1and with times of slaughter after anesthesia of 0, 12, 24 and 48h. It was determined that 48h is the required residual period for total metabolization of menthol in the fishes' organisms. This methodology becomes promising regarding the establishment of protocols to regulatory the use of menthol as an anesthetic in aquaculture.
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: biamesquitacoelho@yahoo.com.br.
authors.forename: Bianca Mesquita Coelho
authors.initials: BM
authors.lastname: Botrel
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: daianynha_cassia@hotmail.com.
authors.forename: Daiane Cássia Pereira
authors.initials: DC
authors.lastname: Abreu
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: adelir@dqi.ufla.br.
authors.forename: Adelir Aparecida
authors.initials: AA
authors.lastname: Saczk
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: matheus.julien@hotmail.com.
authors.forename: Matheus Julien Ferreira
authors.initials: MJ
authors.lastname: Bazana
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: sabrina10mcoelho@yahoo.com.br.
authors.forename: Sabrina Mesquita
authors.initials: SM
authors.lastname: Coelho
authors.affiliation: Department of Animal Science, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: priscila@dzo.ufla.br.
authors.forename: Priscila Vieira
authors.initials: PV
authors.lastname: E Rosa
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: zuy@dqi.ufla.br.
authors.forename: Zuy Maria
authors.initials: ZM
authors.lastname: Magriotis
authors.affiliation: Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil. Electronic address: rafaelmachadolima@hotmail.com.
authors.forename: Rafael Machado Felix
authors.initials: RM
authors.lastname: de Lima
mesh_terms: D000777:Anesthetics / Q000378:metabolism*; D000818:Animals; D005399:Fishes / Q000254:growth & development*; D059022:Food Safety; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008610:Menthol / Q000032:analysis / Q000737:chemistry*
chemical_list: D000777:Anesthetics; D008610:Menthol
keywords: Aquaculture; Chemometrics; Food safety; Residual analysis