title: TsPKA-r: a potential immunodiagnostic antigen for the detection of porcine cysticercosis.
abstract: Cysticercosis, caused by metacestodes of Taenia solium, has a significant soci-economic impact and is of considerable importance in public health. However, there are no specific diagnostic antigens to distinguish between T. solim and Taenia hydatigena. In the present study, cAMP-dependent protein kinase regulatory subunit (TsPKA-r), an excretory/secretary (ES) antigen of T. solium, was used to establish a specific and sensitive diagnostic tool for detection of porcine cysticercosis. The full-length sequence encoding TsPKA-r was amplified by PCR, sequenced and then identified by bioinformatics. The fusion protein with 6×His-tags was expressed in E. coli, purified by Ni Sepharose™ 6 Fast Flow and used to test reactionogenicity by immunoblotting. TsPKA-r based indirect enzyme-linked immunosorbent assays (iELISA) showed good performance in recognition of sera of pigs experimentally infected with T. solium metacestodes, with 93.88% sensitivity and 96.40% specificity. There were no cross-reactions against the sera from pigs experimentally infected with T. hydatigena, Toxoplasma gondii or Trichinella spiralis. These results indicate that the TsPKA-r is a promising immunodiagnostic antigen for detection of porcine cysticercosis.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Guangxue
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Panhong
authors.initials: P
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Aijiang
authors.initials: A
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: zhengyadong@caas.cn.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Public Health, Ministry of Agriculture/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: luoxuenong@caas.cn.
authors.forename: Xuenong
authors.initials: X
authors.lastname: Luo
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D004926:Escherichia coli; D015151:Immunoblotting; D007159:Immunologic Tests; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D041201:Taenia solium
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth
keywords: Immunodiagnostic antigens; T. solium; TsPKA-r; iELISA