title: Avian embryos and related cell lines: A convenient platform for recombinant proteins and vaccine production.
abstract: Chick embryos are a significant historical research model in basic and applied sciences. The embryonated eggs have been used for virus inoculation in order to vaccine production for nearly a century. Recently, avian eggs and cell lines derived from embryonated eggs have found wide application in biotechnology. This review will discuss about the unique characteristics of avian eggs in terms of safety, large scale and economical production of recombinant proteins. This system also provides the human-like glycosylation on target proteins and therefore can be considered as a suitable host for biomanufacturing of humanized monoclonal antibodies and therapeutic proteins. Avian derived cell lines are an alternative for rapid vaccine manufacturing during a pandemic. Based on the latest knowledge in cell and animal transgenesis, the currently available germ cell-mediated gene transfer system provides a more efficient strategy in gene targeting and creation of transgenic birds that lead to advancements in industrial, biotechnology, and biological research applications. This review covers the recent development of avian fertilized eggs and related cell lines in a variety of human biopharmaceuticals and viral vaccine manufacturing.
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Farzaneh
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Seyedeh-Nafiseh
authors.initials: SN
authors.lastname: Hassani
authors.affiliation: Graduate Physiology Program, Campus Box 7608/321 Scott Hall, Raleigh, NC, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Mozdziak
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.|Department of Developmental Biology, University of Science and Culture, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D006801:Humans; D039904:Pluripotent Stem Cells; D011994:Recombinant Proteins; D014765:Viral Vaccines
chemical_list: D011994:Recombinant Proteins; D014765:Viral Vaccines
keywords: Avian; Pluripotent cell lines; Recombinant proteins; Transgenic chicken; Vaccine