title: Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing.
abstract: With Next Generation Sequencing technologies, high-throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates ofDiabrotica virgifera virgifera(LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binaryBacillus thuringiensisinsecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.
authors.affiliation: Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583-0915, USA. hwang4@unl.edu.
authors.forename: Haichuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center for Biotechnology, School of Biological Sciences, UNL, Lincoln, NE 68583, USA. seyun2@unl.edu.
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Eyun
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. kanikaarora316@gmail.com.
authors.forename: Kanika
authors.initials: K
authors.lastname: Arora
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. STan5@dow.com.
authors.forename: Sek Yee
authors.initials: SY
authors.lastname: Tan
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. PGandra@dow.com.
authors.forename: Premchand
authors.initials: P
authors.lastname: Gandra
authors.affiliation: Center for Biotechnology, School of Biological Sciences, UNL, Lincoln, NE 68583, USA. emoriyama2@unl.edu.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Moriyama
authors.affiliation: Monsanto, St. Louis, MO 63167, USA. chitvank@gmail.com.
authors.forename: Chitvan
authors.initials: C
authors.lastname: Khajuria
authors.affiliation: Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583-0915, USA. jessica.jurzenski@fhueng.com.
authors.forename: Jessica
authors.initials: J
authors.lastname: Jurzenski
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. HLi2@dow.com.
authors.forename: Huarong
authors.initials: H
authors.lastname: Li
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. Mmdonahue@dow.com.
authors.forename: Maia
authors.initials: M
authors.lastname: Donahue
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. KNarva@dow.com.
authors.forename: Ken
authors.initials: K
authors.lastname: Narva
authors.affiliation: Entomology and Nematology Department, University of Florida, Gainesville, FL 32611-0620, USA. bsiegfried1@ufl.edu.
authors.forename: Blair
authors.initials: B
authors.lastname: Siegfried
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001517:Coleoptera / Q000235:genetics*; D007814:Larva; D012329:RNA, Bacterial / Q000032:analysis; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D012329:RNA, Bacterial
keywords: Bt challenge; Diabrotica virgifera virgifera; Next Generation Sequencing (NGS); differential gene expression