title: Reporter Systems to Study HTLV-1 Transmission.
abstract: The retrovirus Human T-lymphotropic virus type 1 (HTLV-1) preferentially infects CD4+T-cells via cell-to-cell transmission, while cell-free infection of T-cells is inefficient. Substantial insights into the different routes of transmission have largely been obtained by imaging techniques or by flow cytometry. Recently, strategies to quantify infection events with HTLV-1 improved. In this chapter, we present two different methods to quantitate virus transmission. Both methods are based on measuring gene activity of luciferase with a cost-saving in-house luciferase assay. First, we established a reporter Jurkat T-cell line carrying a luciferase gene under the control of the HTLV-1 core promoter U3R. Upon co-culture with chronically HTLV-1-infected T-cell lines, reporter cells are infected, and upon expression of the viral transactivator Tax, the viral promoter is activated resulting in enhanced luciferase activity. However, this assay as presented here does not exclude cell fusion as the mechanism allowing intracellular Tax-dependent activation of luciferase gene expression. Therefore, we describe a second method, the single-cycle replication-dependent reporter system developed by Mazurov et al. (PLoS Pathog 6:e1000788, 2010) that allows quantitation of HTLV-1 infection in co-cultured cells. Taken together, both methods facilitate quantitation of HTLV-1 transmission and will help to unravel pathways required for cell-to-cell transmission on a quantitative basis.
authors.affiliation: Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, 91054, Erlangen, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Gross
authors.affiliation: Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, 91054, Erlangen, Germany. aakress@viro.med.uni-erlangen.de.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Thoma-Kress
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D017930:Genes, Reporter; D015490:HTLV-I Infections / Q000235:genetics / Q000378:metabolism* / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008156:Luciferases
keywords: Cell-to-cell transmission; Co-culture; HTLV-1; Single-cycle replication-dependent reporter vector; Tax; Transmission; U3R