title: VopE, aVibrio choleraeType III Effector, Attenuates the Activation of CWI-MAPK Pathway in Yeast Model System.
abstract: VopE, a mitochondrial targeting T3SS effector protein ofVibrio cholerae, perturbs innate immunity by modulating mitochondrial dynamics. In the current study, ectopic expression of VopE was found to be toxic in a yeast model system and toxicity was further aggravated in the presence of various stressors. Interestingly, a VopE variant lacking predicted mitochondrial targeting sequence (MTS) also exhibited partial lethality in the yeast system. With the aid of yeast genetic tools and different stressors, we have demonstrated that VopE and its derivative VopEΔMTSmodulate cell wall integrity (CWI-MAPK) signaling pathway and have identified several critical residues contributing to the lethality of VopE. Furthermore, co-expression of two effectors VopEΔMTSand VopX, interfering with the CWI-MAPK cellular pathway can partially suppress the VopX mediated yeast growth inhibition. Taken together, these results suggest that VopE alters signaling through the CWI-MAPK pathway, and demonstrates the usefulness of yeast model system to gain additional insights on the functionality of VopE.
authors.affiliation: Molecular Biology and Microbial Physiology, Institute of Microbial Technology Chandigarh, India.
authors.forename: Leela K
authors.initials: LK
authors.lastname: Bankapalli
authors.affiliation: Molecular Biology and Microbial Physiology, Institute of Microbial Technology Chandigarh, India.
authors.forename: Rahul C
authors.initials: RC
authors.lastname: Mishra
authors.affiliation: Molecular Biology and Microbial Physiology, Institute of Microbial Technology Chandigarh, India.
authors.forename: Saumya
authors.initials: S
authors.lastname: Raychaudhuri
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D002473:Cell Wall / Q000502:physiology; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D020935:MAP Kinase Signaling System; D050296:Microbial Viability; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D014734:Vibrio cholerae / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D037521:Virulence Factors
keywords: GAPs; MAPK signaling pathway; T3SS effector; Vibrio cholerae; stressors; suppression analysis; yeast mitochondria