title: miRNA-101-1 and miRNA-221 expressions and their polymorphisms as biomarkers for early diagnosis of hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is the fifth most common malignant tumor with an increasing incidence. Hepatitis C virus (HCV) is one of the major risk factors that lead to HCC development. MicroRNAs are conserved non-coding RNAs which regulate gene expression at the posttranscriptional level. They have been recently identified as important regulators that affect carcinogenesis. Of these miRNAs, are miR-221 and miR-101-1, which their aberrant expressions have been reported to play an important role in HCC.
  
  
  
  In this study, we investigated the association between miR-221 and miR-101-1 polymorphisms and their expressions and the early prediction of HCC in HCV infected patients. Quantitative real-time PCR (qPCR) was done to estimate the expression levels of miRNA-221 and miRNA-101-1 in serum. To detect the genotyping of miR-221 and miR-101-1 related SNPs, DNA was extracted. Then, genotyping was performed using real-time PCR.
  
  
  
  We found that rs7536540 polymorphism in miR-101-1 is significantly associated with development of HCC. In addition, our results showed no significant association between rs17084733 polymorphism in miR-221 and HCC occurrence. We confirmed the upregulation of miR-221 and the downregulation of miR-101-1 in HCC. As regards HCV patients, miR-221and miR-101-1 were found to be upregulated.
  
  
  
  Both miR-221 and miR-101-1 expression levels may be useful as noninvasive diagnostic biomarkers for early prediction of HCC among HCV patients.
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt. Electronic address: olfatshaker@yahoo.com.
authors.forename: Olfat
authors.initials: O
authors.lastname: Shaker
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.
authors.forename: Maha
authors.initials: M
authors.lastname: Alhelf
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.
authors.forename: George
authors.initials: G
authors.lastname: Morcos
authors.affiliation: Endemic Medicine and Hepatogastroenterology Department, Faculty of Medicine, Cairo University, Egypt.
authors.forename: Aisha
authors.initials: A
authors.lastname: Elsharkawy
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000209:etiology / Q000235:genetics / Q000821:virology; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016174:Hepacivirus / Q000472:pathogenicity / Q000502:physiology; D019698:Hepatitis C, Chronic / Q000150:complications / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000235:genetics / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C534072:MIRN101 microRNA, human; C525709:MIR221, human
keywords: HCC; HCV; SNPs; miR-101-1; miR-221