title: The effects of sclerostin antibody plus parathyroid hormone (1-34) on bone formation in ovariectomized rats.
abstract: Previous studies have demonstrated the effects of sclerostin antibody (Scl-Ab) and parathyroid hormone (1-34, PTH) on healing in osteoporosis; however, reports about the combined effects of Scl-Ab plus PTH on osteoporosis are limited. This study was designed to investigate the impact of combined treatment with Scl-Ab and PTH on osteoporosis healing in ovariectomized (OVX) rats. After bilateral ovariectomy, 12 weeks were allowed to pass for the establishment of standard conditions for osteoporosis in animal models. The rats then randomly received a vehicle (control), Scl-Ab (25 mg/kg body weight, twice weekly), PTH (60 μg/kg, three times per week) or PTH plus Scl-Ab until death at 12 weeks. The blood and distal femurs of the rats were harvested for evaluation. The results of treatment for osteoporosis were evaluated by serum analysis, histology, microcomputed tomography (micro-CT) and biomechanical tests. Results from this study indicated that PTH + Scl-Ab had stronger effects on the prevention and treatment of osteoporosis than either of the monotherapies in OVX rats. The PTH + Scl-Ab produced the strongest effects on bone volume fraction (BV/TV), bone trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular spacing (Tb.Sp), bone mineral density (BMD) and strength of distal femurs and increased the levels of procollagen type I N‑terminal propeptide (PINP) and osteocalcin. In contrast, monotherapy with PTH or Scl-Ab showed no differences between treated groups in the assessment of the metaphysis of contralateral femurs by histology, serum, biomechanical tests and micro-CT. These results seem to indicate that Scl-Ab plus PTH has an additive effect on osteoporosis in OVX rats.
authors.affiliation: Southern Medical University, 510515, Guangzhou, Guangdong, China.|Department of orthopaedics, XianNing Central hospital, The First Affiliated Hospital of Hubei University of Science and Technology, 437100, XianNing, Hubei, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Southern Medical University, 510515, Guangzhou, Guangdong, China. jianyi8286@163.com.|Department of orthopaedics, Wuhan General Hospital of Guangzhou Military Area Commands affiliated Southern Medical University, 627 Wuluo Road, 437000, Wuhan, Hubei, China. jianyi8286@163.com.
authors.forename: Xian-Hua
authors.initials: XH
authors.lastname: Cai
authors.affiliation: Department of orthopaedics, XianNing Central hospital, The First Affiliated Hospital of Hubei University of Science and Technology, 437100, XianNing, Hubei, China.
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Qin
authors.affiliation: Department of orthopaedics, XianNing Central hospital, The First Affiliated Hospital of Hubei University of Science and Technology, 437100, XianNing, Hubei, China.
authors.forename: Yan-Xi
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology*; D015519:Bone Density / Q000187:drug effects; D019485:Bone Morphogenetic Proteins / Q000276:immunology*; D005260:Female; D005819:Genetic Markers / Q000276:immunology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
chemical_list: D000906:Antibodies; D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; D010281:Parathyroid Hormone; C486031:Sost protein, rat
keywords: Animal model; Bone mineral density; Cancellous bone; Distal femur; Osteoporosis