title: MicroRNA-455-3p functions as a tumor suppressor by targeting eIF4E in prostate cancer.
abstract: MicroRNAs (miRNAs) are strongly implicated in various cancers, including prostate cancer. Recently, microRNA-455-3p (miR-455-3p) has been shown to be aberrantly expressed in many tumor tissues, but its functions in tumorigenesis remain unknown. In this study, we investigated the role of miR-455-3p in prostate cancer. We found that miR-455-3p is markedly downregulated in prostate cancer cell lines and clinical tumor specimens. Gain-of-function and loss-of-function studies showed that miR-455-3p promotes prostate cancer cell growth both in vitro and in vivo. Bioinformatics analysis and Luciferase reporter assays demonstrated that miR-455-3p directly targets and suppresses eIF4E, the rate-limiting factor for cap-dependent translation, which plays important roles in the initiation and progression of prostate cancers. Further studies demonstrated that miR-455-3p inhibits cap-dependent translation and the proliferation of prostate cancer cells through targeting eIF4E. Taken together, our findings suggest that miR-455-3p functions as a tumor suppressor by directly targeting eIF4E in prostate carcinogenesis and may be used as a potential target for therapeutic intervention in prostate cancer.
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The Third Affiliated Hospital, Inner Mongolia Medical University, Baotou, Inner Mongolia, P.R. China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yun
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Baotou Center for Disease Control and Prevention, Baotou, Inner Mongolia, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
authors.affiliation: Department of Urinary Surgery of The Fourth Hospital of Baotou, Baotou, Inner Mongolia, P.R. China.
authors.forename: Haishan
authors.initials: H
authors.lastname: Jiang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019295:Computational Biology / Q000379:methods; D015536:Down-Regulation; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics*; D039562:Eukaryotic Initiation Factor-4F / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; D039561:Eukaryotic Initiation Factor-4E; D039562:Eukaryotic Initiation Factor-4F; C583404:MIRN455 microRNA, human; D035683:MicroRNAs