title: Isolation and characterization of rice cesium transporter genes from a rice-transporter-enriched yeast expression library.
abstract: A considerable portion of agricultural land in central-east Japan has been contaminated by radioactive material, particularly radioactive Cs, due to the industrial accident at the Fukushima Daiichi nuclear power plant. Understanding the mechanism of absorption, translocation and accumulation of Cs+in plants will greatly assist in developing approaches to help reduce the radioactive contamination of agricultural products. At present, however, little is known regarding the Cs+transporters in rice. A transporter-enriched yeast expression library was constructed and the library was screened for Cs+transporter genes. The 1452 full length cDNAs encoding transporter genes were obtained from the Rice Genome Resource Center and 1358 clones of these transporter genes were successively subcloned into yeast expression vectors; which were then transferred into yeast. Using this library, both positive and negative selection screens can be performed, which have not been previously possible. The constructed library is an excellent tool for the isolation of novel transporter genes. This library was screened for clones that were sensitive to Cs+using a SD-Gal medium containing either 30 or 70 mM CsCl; resulting in the isolation of 13 Cs+sensitive clones.137Cs absorption experiments were conducted and confirmed that all of the identified clones were able to absorb137Cs. A total of 3 potassium transporters, 2 ABC transporters and 1 NRAMP transporter were among the 13 identified clones.
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaki
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otani
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ono
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Mimura
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Koshiro
authors.initials: K
authors.lastname: Oda
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Minamii
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Matsumoto
authors.affiliation: Microtubule Cytoskeleton Laboratory, Francis Crick Institute Ringgold Standard Institution, London, UK.
authors.forename: Yuzy
authors.initials: Y
authors.lastname: Matsuo
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
authors.affiliation: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
authors.forename: Takashi
authors.identifier: http://orcid.org/0000-0001-8349-3290
authors.initials: T
authors.lastname: Akihiro
mesh_terms: D002586:Cesium / Q000378:metabolism*; D002588:Cesium Radioisotopes / Q000032:analysis; D002712:Chlorides / Q000378:metabolism; D015723:Gene Library; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D002588:Cesium Radioisotopes; D002712:Chlorides; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D002586:Cesium; C028019:cesium chloride