title: Isolation and screening of proangiogenic and antiangiogenic metabolites producing rare actinobacteria from soil.
abstract: OBJECTIVE
  Angiogenesis is a physiological process that has important impacts on the pathology and healing of various diseases, and its induction or inhibition by bioactive actinobacterial metabolites can help the treatment of some diseases. In this study, the effects of actinobacterial extract in the process of angiogenesis have been explored.
  
  
  RESULTS
  In this research, proangiogenic and antiangiogenic metabolites producing actinobacteria were isolated from soil samples and their fermentation broth were extracted and after evaluation of their toxicity by MTT assay, antiangiogenic and proangiogenic activities were screened against human umbilical vein endothelial cells (HUVECs) by in vitro tube formation and migration assay. Isolated strains were identified through molecular techniques. The results showed that Nocardiopsis arvandica UTMC 103 and Nonomuraea sp. UTMC 2180 extracts had a high potential of anti-angiogenic activity on HUVECs.
  
  
  CONCLUSIONS
  For the first time proangiogenic potency of a rare actinobacterium, Kribbella sp. UTMC 522, was reported, and N. arvandica UTMC 103 and Nonomuraea sp. UTMC 2180 extracts inhibits the proliferation, migration and angiogenesis activity of HUVECs with reasonable potency.
  
  
  CONCLUSIONS
  Metabolites of the introduced rare actinobacteria are potent proangiogenic and angiogenic inhibitors. Identification of angiogenic-antiangiogenic mechanisms and purification of the extracts would be useful in therapeutic angiogenesis.
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Y
authors.initials: Y
authors.lastname: Azarakhsh
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.|Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.
authors.forename: F
authors.identifier: http://orcid.org/0000-0003-0286-5342
authors.initials: F
authors.lastname: Mohammadipanah
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: S M
authors.identifier: http://orcid.org/0000-0002-5746-9593
authors.initials: SM
authors.lastname: Nassiri
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: V
authors.initials: V
authors.lastname: Siavashi
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.|Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Hamedi
mesh_terms: D039903:Actinobacteria / Q000302:isolation & purification / Q000378:metabolism*; D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005285:Fermentation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D012988:Soil Microbiology
chemical_list: D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors
keywords: Kribbella; Nocardiopsis arvandica; Nonomuraea; actinobacteria; angiogenesis; metabolites; soil; vasculogenesis