title: Tailored Chemical Properties of 4-Arm Star Shaped Poly(d,l-lactide) as Cell Adhesive Three-Dimensional Scaffolds.
abstract: Biodegradable poly(lactic acid) (PLA) is widely used to fabricate 3D scaffolds for tissue regeneration. However, PLA lacks cell adhering functional moieties, which limit its successful application in tissue engineering. Herein, we have tailored the cell adhesive properties of star shaped poly(d,l-lactide) (ss-PDLLA) by grafting gelatin to their 4 arms. Grafting of gelatin on PDLLA backbone was confirmed by1H NMR and FTIR. The synthesized star shaped poly(d,l-lactide)-b-gelatin (ss-pLG) exhibited enhanced wettability and protein adsorption. The modification also facilitated better cell adhesion and proliferation on their respective polymer coated 2D substrates, compared to their respective unmodified ss-PDLLA. Further, 3D scaffolds were fabricated from gelatin grafted and unmodified polymers. The fabricated scaffolds were shown to be cytocompatible to 3T3-L1 cells and hemocompatible to red blood cells (RBCs). Cell proliferation was increased up to 2.5-fold in ss-pLG scaffolds compared to ss-PDLLA scaffolds. Furthermore, a significant increase in cell number reveals a high degree of infiltration of cells into the scaffolds, forming a viable and healthy 3D interconnected cell community. In addition to that, burst release of docetaxal (DTX) was observed from ss-pLG scaffolds. Hence, this new system of grafting polymers followed by fabricating 3D scaffolds could be utilized as a successful approach in a variety of applications where cell-containing depots are used.
authors.forename: Chelladurai Karthikeyan
authors.initials: CK
authors.lastname: Balavigneswaran
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Mahto
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology , Mathura road, New Delhi-110025, India.
authors.forename: Bano
authors.initials: B
authors.lastname: Subia
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology , Mathura road, New Delhi-110025, India.|Academy of Scientific and Innovative Research (AcSIR) , Anusandhan Bhavan, 2 Rafi Marg, New Delhi-110001, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Prabhakar
authors.affiliation: Department of Chemistry, Institute of Science, Banaras Hindu University , Varanasi-221005, India.
authors.forename: Kheyanath
authors.initials: K
authors.lastname: Mitra
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology , Mathura road, New Delhi-110025, India.|Academy of Scientific and Innovative Research (AcSIR) , Anusandhan Bhavan, 2 Rafi Marg, New Delhi-110001, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Rao
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology , Mathura road, New Delhi-110025, India.|Academy of Scientific and Innovative Research (AcSIR) , Anusandhan Bhavan, 2 Rafi Marg, New Delhi-110001, India.
authors.forename: Munia
authors.initials: M
authors.lastname: Ganguli
authors.affiliation: Department of Chemistry, Institute of Science, Banaras Hindu University , Varanasi-221005, India.
authors.forename: Biswajit
authors.identifier: 0000-0001-5858-9004
authors.initials: B
authors.lastname: Ray
authors.forename: Pralay
authors.initials: P
authors.lastname: Maiti
authors.forename: Nira
authors.identifier: 0000-0002-3700-5206
authors.initials: N
authors.lastname: Misra
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D000077143:Docetaxel; D004912:Erythrocytes / Q000166:cytology; D005780:Gelatin; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D043823:Taxoids / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D043823:Taxoids; D000077143:Docetaxel; C033616:poly(lactide); D005780:Gelatin