title: Quantification of inositol phosphates in almond meal and almond brown skins by HPLC/ESI/MS.
abstract: The extraction and measurement of all six forms of inositol phosphates (InsPs) in almond meal and brown skins were improved from existing methods by pH adjustment, supplementation of EDTA, and rapid analysis via anion-exchange high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. The quantity of InsPs in six major almond cultivars ranged from 8 to 12μmol/g in the meal and 5 to 14μmol/g in the brown skins. InsP6was the dominant form, but lower forms still accounted for ∼20% of the total InsPs molar concentration in a majority of the samples. InsPs contributed 32-55% of the organic phosphorus content and 20-38% of the total phosphorus content in the meal. In brown skins, these ranges were 44-77% and 30-52%, respectively. The successful application of this analytical method with almonds demonstrates its potential use for re-examination of the reported phytic acid contents in many other tree nuts, legumes, grains, and complex foods.
authors.affiliation: Department of Food Science & Technology, College of Agricultural and Environmental Sciences, The University of Georgia, 100 Cedar Street, Athens, GA 30602, USA.
authors.forename: Quynh H
authors.initials: QH
authors.lastname: Duong
authors.affiliation: Department of Food Science & Technology, College of Agricultural and Environmental Sciences, The University of Georgia, 100 Cedar Street, Athens, GA 30602, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Clark
authors.affiliation: Almond Board of California, 1150 Ninth Street, Suite, 1500, Modesto, CA 95354, USA.
authors.forename: Karen G
authors.initials: KG
authors.lastname: Lapsley
authors.affiliation: Department of Food Science & Technology, College of Agricultural and Environmental Sciences, The University of Georgia, 100 Cedar Street, Athens, GA 30602, USA. Electronic address: rpegg@uga.edu.
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Pegg
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D007295:Inositol Phosphates / Q000737:chemistry*; D000068181:Prunus dulcis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D007295:Inositol Phosphates
keywords: Almond brown skins; Almond meal; HPLC-ESI-MS; Inositol phosphate (InsP); Ion-exchange HPLC; Phytic acid