title: Floral parts of Gomphrena globosa L. as a novel alternative source of betacyanins: Optimization of the extraction using response surface methodology.
abstract: The present study describes a novel mechanical process for the pigmented parts of Gomphrena globosa L. The effects of the variables of the maceration extraction of betacyanins have not been properly described. Therefore, this study also aims to optimize the conditions that maximize betacyanins extraction from G. globosa as an alternative source. Assisted by response surface methodology, an experimental design was developed for testing the extraction variables (time, temperature, ethanol-water proportion and solid-liquid ratio). The responses used were betacyanins quantification (by HPLC-PDA-MS/ESI and spectrophotometric analysis), the extraction-yield and the colour intensity of the produced powder. The betacyanins identified were gomphrenin and isogomphrenin II and III. The highest betacyanins content (∼45mg/g) was obtained by 165min, 25°C, 0% of ethanol and 5g/L of solid-liquid ratio. The betacyanins content from the floral parts of G. globosa is higher than those normally found in other sources highlighting its industrial application.
authors.affiliation: Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5300-253 Bragança, Portugal; Dpto. Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense de Madrid (UCM), Plaza Ramón y Cajal, s/n, E-28040 Madrid, Spain.
authors.forename: Custódio Lobo
authors.initials: CL
authors.lastname: Roriz
authors.affiliation: Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5300-253 Bragança, Portugal; Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials (LSRE-LCM), Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1134, 5301-857 Bragança, Portugal. Electronic address: lillian@ipb.pt.
authors.forename: Lillian
authors.initials: L
authors.lastname: Barros
authors.affiliation: Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5300-253 Bragança, Portugal; Nutrition and Bromatology Group, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E32004 Ourense, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Prieto
authors.affiliation: Dpto. Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense de Madrid (UCM), Plaza Ramón y Cajal, s/n, E-28040 Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Morales
authors.affiliation: Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5300-253 Bragança, Portugal. Electronic address: iferreira@ipb.pt.
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry; D050859:Betacyanins / Q000032:analysis / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*
chemical_list: D050859:Betacyanins; D010936:Plant Extracts
keywords: Betacyanins extraction; Food colorants; Gomphrena globosa L.; Optimization; Response surface methodology