title: Age-dependent systemic DNA damage in early Type 2 Diabetes mellitus.
abstract: Oxidative stress, capable of eliciting damage to various biomolecules including DNA, is a recognized component of diabetes mellitus and its complications. Metabolic syndrome (MetS) is associated with the development of type 2 diabetes mellitus (T2DM), as well as other unfavorable outcomes. The aim of this study was to elucidate the role of oxidative stress in the development of T2DM, by investigating association of oxidative DNA damage with metabolic parameters in subjects with MetS and early T2DM. Selected anthropometric and biochemical parameters of MetS, inflammation and oxidative DNA damage: body mass index (BMI), fatty liver index (FLI), waist circumference (WC), total cholesterol, HDL and LDL-cholesterol, gamma-glutamyl transpeptidase (GGT), uric acid, C-reactive protein (CRP), total leukocyte/neutrophil count, and urinary 8-hidroxy-deoxyguanosine (u-8-OHdG) were assessed in male subjects with MetS and both younger (≤55 years) and older (>55 years) subjects with T2DM of short duration without complications. BMI, FLI, WC, total and LDL-cholesterol and uric acid were higher, while the u-8-OHdG was lower in MetS group, when compared to older T2DM subjects. None of these parameters were different neither between MetS and younger T2DM, nor between two sub-groups of subjects with T2DM. Values of CRP, HDL-cholesterol, triglycerides, GGT, leukocytes and neutrophils were not different between all examined groups of subjects. Higher 8-OHdG in older subjects with T2DM suggests that both aging process and diabetes could contribute to the development of DNA damage. Oxidative DNA damage cannot serve as an universal early marker of T2DM.
authors.affiliation: Department of Internal Medicine, General Hospital Brežice, Slovenia.
authors.forename: Dinko
authors.initials: D
authors.lastname: Rogulj
authors.affiliation: General Hospital Varaždin, Department for extending and palliative care Novi Marof, Croatia.
authors.forename: Ismail
authors.initials: I
authors.lastname: El Aklouk
authors.affiliation: NMR Centre, Institute Rudjer Bošković, Zagreb, Croatia.
authors.forename: Paško
authors.initials: P
authors.lastname: Konjevoda
authors.affiliation: Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, University Hospital Merkur, Zagreb, Croatia.
authors.forename: Spomenka
authors.initials: S
authors.lastname: Ljubić
authors.affiliation: Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, University Hospital Merkur, Zagreb, Croatia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Pibernik Okanović
authors.affiliation: Polyclinic Sunce, Zagreb, Croatia.
authors.forename: Ante
authors.initials: A
authors.lastname: Barbir
authors.affiliation: Polyclinic Sunce, Zagreb, Croatia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Luburić
authors.affiliation: Department of Internal Medicine, University Hospital Centre Split, Split, Croatia.
authors.forename: Maja
authors.initials: M
authors.lastname: Radman
authors.affiliation: Department of Internal Medicine, University Hospital Sveti Duh, Zagreb, Croatia.
authors.forename: Ninoslav
authors.initials: N
authors.lastname: Budinski
authors.affiliation: Department of Laboratory Medicine, Scientific Research Unit, University Hospital Merkur, Zagreb, Croatia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Vučić Lovrenčić
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000235:genetics* / Q000473:pathology; D001794:Blood Pressure; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D012307:Risk Factors
chemical_list: D008076:Cholesterol, HDL; D002097:C-Reactive Protein; D002784:Cholesterol
keywords: DNA damage; metabolic syndrome; type 2 diabetes mellitus; urinary 8-OHdG