title: Highly sensitive method for the quantification of trans-linolenic acid isomers in trilinolenin of edible oils using an ionic liquid capillary column.
abstract: BACKGROUND
  The polarities of linolenic acid isomers are very similar, and only a few studies to date have attempted to separate α-linolenic acid (ALA) isomers completely. The aim of this study was to fill this gap by developing and validating an accurate method for the analysis of ALA isomers in trilinolenin at 200, 220 and 240 °C using a gas chromatograph-flame ionization detector equipped with an SLB-IL111 capillary column.
  
  
  RESULTS
  Results showed that eight ALA isomer standards were separated effectively using these optimized gas chromatographic conditions. The coefficient of determination was r2> 0.9994 in the linear range of each ALA isomer. The obtained limits of detection and limits of quantification of the ALA isomers were 0.02-0.08 ppm and 0.05-0.22 ppm, respectively. A high degree of reproducibility and percent recoveries between 96.2% and 106.5%, with coefficients of variation ranging from 0.82% to 0.97%, were achieved.
  
  
  CONCLUSIONS
  The developed method has been successfully applied to the analysis of ALA isomers in heated pure trilinolenin as well as to trilinolenin in various edible oils, and the TALA isomerization pathways in heated trilinolenin were verified. © 2017 Society of Chemical Industry.
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Qin
authors.identifier: http://orcid.org/0000-0002-9806-3119
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Qingpeng
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Ha
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D006358:Hot Temperature; D052578:Ionic Liquids; D007536:Isomerism; D057230:Limit of Detection; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000737:chemistry*; D017962:alpha-Linolenic Acid / Q000032:analysis* / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D010938:Plant Oils; D014280:Triglycerides; D017962:alpha-Linolenic Acid; C101314:trilinolenin
keywords: fatty acid isomers; gas chromatography; ionic liquid capillary column; method; trilinolenin