title: Fast assembling of neuron fragments in serial 3D sections.
abstract: Reconstructing neurons from 3D image-stacks of serial sections of thick brain tissue is very time-consuming and often becomes a bottleneck in high-throughput brain mapping projects. We developed NeuronStitcher, a software suite for stitching non-overlapping neuron fragments reconstructed in serial 3D image sections. With its efficient algorithm and user-friendly interface, NeuronStitcher has been used successfully to reconstruct very large and complex human and mouse neurons.
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.|Cortical Architecture Imaging and Discovery Laboratory, Department of Computer Science and Bioimaging Research Center, The University of Georgia, Athens, GA, USA.
authors.forename: Hanbo
authors.identifier: http://orcid.org/0000-0002-8094-1761
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neuroscience, Columbia University, New York, NY, USA.
authors.forename: Daniel Maxim
authors.initials: DM
authors.lastname: Iascone
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Nuno Maçarico
authors.initials: NM
authors.lastname: da Costa
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Ed S
authors.initials: ES
authors.lastname: Lein
authors.affiliation: Cortical Architecture Imaging and Discovery Laboratory, Department of Computer Science and Bioimaging Research Center, The University of Georgia, Athens, GA, USA.
authors.forename: Tianming
authors.initials: T
authors.lastname: Liu
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA. hanchuanp@alleninstitute.org.
authors.forename: Hanchuan
authors.initials: H
authors.lastname: Peng
keywords: Alignment; Neuron; Neuron stitcher; Reconstruct morphology; Stitch; Vaa3D