title: Complement component 3: characterization and association with mastitis resistance in Egyptian water buffalo and cattle.
abstract: Mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. Complement component C3 plays a major role as a central molecule of the complement cascade involving in killing of microorganisms, either directly or in cooperation with phagocytic cells. C3 cDNA were isolated, from Egyptian buffalo and cattle, sequenced and characterized. The C3 cDNA sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. Buffalo and cattle C3 cDNAs share 99% of sequence identity with each other. The 4986 bp open reading frame in buffalo encodes a putative protein of 1661 amino acids-as in cattle-and includes all the functional domains. Further, analysis of the C3 cDNA sequences detected six novel single-nucleotide polymorphisms (SNPs) in buffalo and three novel SNPs in cattle. The association analysis of the detected SNPs with milk somatic cell score as an indicator of mastitis revealed that the most significant association in buffalo was found in the C>A substitution (ss: 1752816097) in exon 27, whereas in cattle it was in the C>T substitution (ss: 1752816085) in exon 12. Our findings provide preliminary information about the contribution of C3 polymorphisms to mastitis resistance in buffalo and cattle.
authors.affiliation: Department of Cell Biology, National Research Centre, 12622 Giza, Egypt. nelhalawany2001@hotmail.com.
authors.forename: Nermin
authors.initials: N
authors.lastname: El-Halawany
authors.forename: Shawky A
authors.initials: SA
authors.lastname: Abd-El-Monsif
authors.forename: F M
authors.initials: FM
authors.lastname: Al-Tohamy Ahmed
authors.forename: Lamees
authors.initials: L
authors.lastname: Hegazy
authors.forename: Hamdy
authors.initials: H
authors.lastname: Abdel-Shafy
authors.forename: Magdy A
authors.initials: MA
authors.lastname: Abdel-Latif
authors.forename: Yasser A
authors.initials: YA
authors.lastname: Ghazi
authors.forename: Christiane
authors.initials: C
authors.lastname: Neuhoff
authors.forename: Dessie
authors.initials: D
authors.lastname: Salilew-Wondim
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002020:Buffaloes / Q000235:genetics* / Q000276:immunology; D002417:Cattle; D003176:Complement C3 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D004534:Egypt; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008414:Mastitis, Bovine / Q000235:genetics* / Q000276:immunology; D008958:Models, Molecular; D009154:Mutation; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011487:Protein Conformation; D017422:Sequence Analysis, DNA
chemical_list: D003176:Complement C3; D018076:DNA, Complementary