title: Studies on electrochemical glucose sensing, antimicrobial activity and cytotoxicity of fabricated copper nanoparticle immobilized chitin nanostructure.
abstract: In this study, copper nanoparticle immobilized chitin nanocomposite (CNP/CuNP) was synthesized and used for the development of non-enzymatic electrochemical sensor. The CNP/CuNP was characterized by X-ray diffraction (XRD), fourier transform infra red (FTIR) spectroscopy and high resolution transmission electron microscopy (HRTEM) analysis. The glucose sensing property of CNP/CuNP was investigated by cyclic voltammetry (CV) and chronoamperometry (CA). As a result of the synergistic effect of CNP and CuNP, the modified electrode displayed effective electro-oxidation of glucose in 0.1M NaOH solution. At 0.45V potential the modified electrode showed response towards glucose in the linear range of 1-1000μM with a lowest detection limit of 0.776μM with better selectivity and stability. In addition, the antimicrobial activity of CNP/CuNP was evaluated against bacterial and fungal strains. CNP/CuNP displayed enhanced antimicrobial activity when compared to CNP and CuNP alone. Similarly, cytotoxicity of CNP/CuNP was tested against Artemia salina, which showed no toxic effect in the tested concentration.
authors.affiliation: Department of Biotechnology, Science Campus, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
authors.forename: Dhanasekaran
authors.initials: D
authors.lastname: Solairaj
authors.affiliation: Department of Biotechnology, Science Campus, Alagappa University, Karaikudi 630003, Tamil Nadu, India. Electronic address: rameshthangam@alagappauniversity.ac.in.
authors.forename: Palanivel
authors.initials: P
authors.lastname: Rameshthangam
authors.affiliation: Department of Bioelectronics and Biosensors, Science Campus, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Muthukumaran
authors.affiliation: Department of Bioelectronics and Biosensors, Science Campus, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
authors.forename: Jeyaraj
authors.initials: J
authors.lastname: Wilson
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001156:Artemia / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D002686:Chitin / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D005658:Fungi / Q000187:drug effects; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D002686:Chitin; D003300:Copper; D005947:Glucose
keywords: Antimicrobial; Chitin; Copper; Cytotoxicity; Glucose sensing; Nanocomposite