title: "Molecular Activity Painting": Switch-like, Light-Controlled Perturbations inside Living Cells.
abstract: Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging owing to the lateral diffusion of plasma membrane targeted molecules. Herein we present "molecular activity painting" (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable "painting" of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.
authors.affiliation: Chemical Genomics Centre of the Max-Planck Society, Dortmund, Germany.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department for Systemic Cell Biology, Max Planck Institute of Molecular Physiology and Fakultät für Chemie und Chemische Biologie, Technische Universität Dortmund, Dortmund, Germany.
authors.forename: Muthukumaran
authors.initials: M
authors.lastname: Venkatachalapathy
authors.affiliation: Department for Systemic Cell Biology, Max Planck Institute of Molecular Physiology and Fakultät für Chemie und Chemische Biologie, Technische Universität Dortmund, Dortmund, Germany.
authors.forename: Dominic
authors.initials: D
authors.lastname: Kamps
authors.affiliation: Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Weigel
authors.affiliation: Institute of Nanotechnology (INT) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Ravi
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department for Systemic Cell Biology, Max Planck Institute of Molecular Physiology and Fakultät für Chemie und Chemische Biologie, Technische Universität Dortmund, Dortmund, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Orlich
authors.affiliation: Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Ruben
authors.initials: R
authors.lastname: Garrecht
authors.affiliation: Institute of Nanotechnology (INT) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hirtz
authors.affiliation: Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
authors.affiliation: Chemical Genomics Centre of the Max-Planck Society, Dortmund, Germany.
authors.forename: Yao-Wen
authors.initials: YW
authors.lastname: Wu
authors.affiliation: Department for Systemic Cell Biology, Max Planck Institute of Molecular Physiology and Fakultät für Chemie und Chemische Biologie, Technische Universität Dortmund, Dortmund, Germany.
authors.forename: Leif
authors.identifier: 0000-0002-6559-6496
authors.initials: L
authors.lastname: Dehmelt
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000737:chemistry; D019281:Dimerization; D062069:Inventions / Q000639:trends; D008027:Light; D014157:Transcription Factors / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; C480633:SLC2A4RG protein, human; D014157:Transcription Factors
keywords: Rho GTPases; immobilization; optochemical biology; photochemically induced dimerization; signal transduction