title: Fixation of CO2using the ethylmalonyl-CoA pathway in the photoheterotrophic marine bacterium Dinoroseobacter shibae.
abstract: The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world´s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12Tduring transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Rebenring 56, Braunschweig, D-38106, Germany.
authors.forename: Nelli
authors.initials: N
authors.lastname: Bill
authors.affiliation: Department of Microbial Communication, Helmholtz-Centre for Infection Research (HZI), Inhoffenstrasse 7, Braunschweig, D-38124, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Tomasch
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Rebenring 56, Braunschweig, D-38106, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Riemer
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Rebenring 56, Braunschweig, D-38106, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Müller
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Rebenring 56, Braunschweig, D-38106, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kleist
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Rebenring 56, Braunschweig, D-38106, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Schmidt-Hohagen
authors.affiliation: Department of Microbial Communication, Helmholtz-Centre for Infection Research (HZI), Inhoffenstrasse 7, Braunschweig, D-38124, Germany.
authors.forename: Irene
authors.initials: I
authors.lastname: Wagner-Döbler
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Rebenring 56, Braunschweig, D-38106, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schomburg
mesh_terms: D000214:Acyl Coenzyme A / Q000235:genetics / Q000378:metabolism*; D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002952:Citric Acid Cycle; D008027:Light; D010788:Photosynthesis; D041902:Rhodobacteraceae / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D059467:Transcriptome
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; C102829:ethylmalonyl-coenzyme A; D002245:Carbon Dioxide