title: Genetic and Proteomic characterization of Bile Salt Export Pump (BSEP) in Snake Liver.
abstract: Snake gallbladder, a traditional Chinese medicine, has been believed in various Asian countries to improve visual acuity and alleviate rheumatism. Bile acids, a major component of the gallbladder, are toxic to the liver and kidney in humans and animals due to its detergent effects, while also exhibiting therapeutic effects due to an increase in the gallbladder contractions of muscle strips in patients with cholesterol gallstones. Secretion of bile acids in human and mammals depends on the bile salt export pump (BSEP), a liver-specific adenosine triphosphate (ATP)-binding cassette transporter encoded by ABCB11. However, the presence of BSEP in snakes has not been thoroughly explored. Here we confirm the existence of BSEP and its coding DNA sequence in snakes on both the proteomic and genetic level. This work provides information on the snake ABCB11 sequence and helps further potential genetic manipulation to affect bile salt metabolism. Our study provides the foundation for research on bile acid production from snakes by using modern genetic and proteomic methodologies.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.|The University of Queensland, School of Chemistry &Molecular Bioscience, Brisbane, QLD 4072, Australia.
authors.forename: Xinle
authors.initials: X
authors.lastname: Tan
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Hematology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Hexiu
authors.initials: H
authors.lastname: Su
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.
authors.forename: Mitchell A
authors.initials: MA
authors.lastname: Sullivan
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Jiachun
authors.initials: J
authors.lastname: Chen
mesh_terms: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11 / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D023281:Genomics / Q000379:methods; D008099:Liver / Q000378:metabolism*; D010802:Phylogeny; D040901:Proteomics / Q000379:methods; D017422:Sequence Analysis, DNA; D012911:Snakes / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11