title: The critical role of myeloperoxidase in Streptococcus pneumoniae clearance and tissue damage during mouse acute otitis media.
abstract: We have recently reported that neutrophils play a pivotal role in innate defense against Streptococcus pneumoniae ( Spn) during mouse acute otitis media (AOM). However, the underlying mechanism remains unclear. By constructing models of pneumococcal AOM in C57BL/6 mice and using a specific inhibitor in vivo, we investigated the role of myeloperoxidase (MPO), one of the most important protein components of neutrophils. Experiment results showed a significant increase in MPO production of the recruited neutrophils in Spn-infected mice. Neutrophils killed Spn in a MPO-dependent manner. MPO facilitated the generation of reactive oxygen species (ROS), and consequently promoted Spn clearance at an early stage and exacerbated tissue damage. Moreover, MPO induced neutrophil apoptosis and necrosis, which, in turn, worsened tissue damage. In summary, our study demonstrates that neutrophil MPO plays a paradoxical role in bacterial clearance and tissue damage in pneumococcal AOM.
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.|2 Department of Laboratory Medicine, Chongqing Traditional Chinese Medicine Hospital, Chongqing, People's Republic of China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Jin
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.|3 The Sixth People's Hospital of Chongqing City, Chongqing, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Zimeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Fangmei
authors.initials: F
authors.lastname: Fan
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Ma
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Wenchun
authors.initials: W
authors.lastname: Xu
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: 1 Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine (Ministry of Education), Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D017209:Apoptosis; D001433:Bacteriolysis; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D010033:Otitis Media / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism*; D011008:Pneumococcal Infections / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: D017382:Reactive Oxygen Species; D009195:Peroxidase
keywords: Acute otitis media; apoptosis; myeloperoxidase; reactive oxygen species