title: Stem cells cardiac patch from decellularized umbilical artery improved heart function after myocardium infarction.
abstract: The construction of the high biocompatible biomaterials pretreated with MSC offers a promising strategy to improve the effects of stem cell therapy for the myocardial infarction (MI). However, assembling vascularized three-dimensional (3-D) myocardial tissues remains an enormous challenge. In this study, we optimized the decellularization protocol with the umbilical artery to construct microporous 3-D scaffold which is suitable for the stem cells (SC) proliferation. The SD rats underwent proximal left coronary ligation and a 5-mm diameter microporous SC patch was implanted directly on the infarct area (SC patch group). The LV contractile function, regional myocardial wall compliance, and tissue histology were assessed 4 weeks after patch implantation. The MSC patch integrated to the local heart tissue and the neo-vessels have been observed in the MSC patch. The vessels in the MSC patch were positive for the CD31 (marker for the mature endothelial cells). The left ventricle wall was thicker in the MSC patch group than the control group (p<0.05 vs. empty patch group). And the LVEF has been improved in the MSC patch group than empty patch group (59±6.7% vs. 31±4.5%, p<0.05).
  
  
  CONCLUSIONS
  Our results showed that the implantation of the MSC patch improved cardiac contractile function in heart infarction rat model. The construction of artificial tissue from the decellularized umbilical artery and the MSC may open a promising perspective for the tissue therapy for MI.
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.|The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.
authors.forename: RanRan
authors.initials: R
authors.lastname: Huang
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.
authors.forename: XiaoXia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.
authors.forename: YiMin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: CHU Nancy, Université de Lorraine et l'UMR CNRS IMOPA, Nancy, France.|CHU, Unité de Thérapie Cellulaire, 54511 Vandœuvre-Lès-Nancy, France.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Stoltz
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.
authors.forename: YuJie
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijing, P.R. China.|Beijng Institute of Heart Lung and Blood Vessels Disease, Beijing, P.R. China.
authors.forename: QingYu
authors.initials: Q
authors.lastname: Kong
mesh_terms: D000818:Animals; D006321:Heart / Q000503:physiopathology*; D006352:Heart Ventricles / Q000166:cytology / Q000503:physiopathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014469:Umbilical Arteries / Q000737:chemistry / Q000637:transplantation* / Q000648:ultrastructure
keywords: Mesenchymal stem cells; cardiac patch; heart infarction; umbilical artery decellularization