title: Cellular and molecular mechanism for secretory autophagy.
abstract: Macroautophagy/autophagy plays a role in unconventional secretion of leaderless cytosolic proteins. Whether and how secretory autophagy diverges from conventional degradative autophagy is unclear. We have shown that the prototypical secretory autophagy cargo IL1B/IL-1β (interleukin 1 β) is recognized by TRIM16, and that this first to be identified secretory autophagy receptor interacts with the R-SNARE SEC22B to jointly deliver cargo to the MAP1LC3B-II-positive sequestration membranes. Cargo secretion is unaffected by knockdowns of STX17, a SNARE catalyzing autophagosome-lysosome fusion as a prelude to cargo degradation. Instead, SEC22B in combination with plasma membrane syntaxins completes cargo secretion. Thus, secretory autophagy diverges from degradative autophagy by using specialized receptors and a dedicated SNARE machinery to bypass fusion with lysosomes.
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kimura
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Jingyue
authors.initials: J
authors.lastname: Jia
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Aurore
authors.initials: A
authors.lastname: Claude-Taupin
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Suresh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Choi
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Yuexi
authors.initials: Y
authors.lastname: Gu
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Michal
authors.initials: M
authors.lastname: Mudd
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dupont
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Shanya
authors.initials: S
authors.lastname: Jiang
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Peters
authors.affiliation: b Department of Physics and Astronomy , University of New Mexico Northeast , Albuquerque , NM , USA.
authors.forename: Farzin
authors.initials: F
authors.lastname: Farzam
authors.affiliation: c Molecular Cancer Research Group , Institute of Medical Biology, University of Tromsø-The Arctic University of Norway , Tromsø , Norway.
authors.forename: Ashish
authors.initials: A
authors.lastname: Jain
authors.affiliation: b Department of Physics and Astronomy , University of New Mexico Northeast , Albuquerque , NM , USA.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Lidke
authors.affiliation: d Stanford University Mass Spectrometry , Stanford University , Stanford , CA , USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Adams
authors.affiliation: c Molecular Cancer Research Group , Institute of Medical Biology, University of Tromsø-The Arctic University of Norway , Tromsø , Norway.
authors.forename: Terje
authors.initials: T
authors.lastname: Johansen
authors.affiliation: a Department of Molecular Genetics and Microbiology , University of New Mexico Health Sciences Center, Northeast , Albuquerque , NM , USA.
authors.forename: Vojo
authors.identifier: http://orcid.org/0000-0002-3624-5208
authors.initials: V
authors.lastname: Deretic
mesh_terms: D001343:Autophagy; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008561:Membrane Fusion; D008954:Models, Biological; D010588:Phagosomes / Q000378:metabolism; D050600:SNARE Proteins / Q000378:metabolism; D055571:Secretory Pathway
chemical_list: D050600:SNARE Proteins
keywords: IL1B; SNAREs; TRIMs; ferritin; galectins; inflammasome; unconventional secretion