title: Protective Role of Maternal P.VAL158MET Catechol-O-Methyltransferase Polymorphism against Early-Onset Preeclampsia and its Complications.
abstract: BACKGROUND
  Up until now there have been contradictory data about the association between p.Val158Met catechol-O-methyltransferase (COMT) polymorphism and risk of preeclampsia (PE). The goal of this study was to assess the potential correlation between p.Val158MetCOMTpolymorphism and risk of early-onset PE, risk of a severe form of early-onset PE, as well as risk of small-for-gestational-age (SGA) complicating PE.
  
  
  METHODS
  The study included 47 early-onset PE patients and 47 control cases. Forty-seven early-onset PE patients were grouped by disease severity (33 patients with a severe form and 14 patients without severe features) and secondly by size for gestational age (12 patients with appropriate-for-gestational-age (AGA) and 35 patients with SGA size). p.Val158Met polymorphism was genotyped by PCR-RFLP analysis.
  
  
  RESULTS
  Allele analysis showed significant difference inCOMTallele distribution between early-onset PE and control group as well as early-onset PE SGA and controls (p=0.04057 and p=0.0411 respectively). A statistically significant distribution difference between the severe form and form without severe features of early-onset PE patients was not observed (p>0.05). The highest difference observed was in the allele recessive model whereCOMTMetMet genotype was associated with decreased risk of early-onset PE (OR=0.281; 95%CI = 0.092-0.7836) and PE complications including severe early-onset PE (OR= 0.304; 95%CI=0.086-0.944) and SGA early-onset PE (OR=0.284; 95%CI=0.081-0.874).
  
  
  CONCLUSIONS
  COMTmay be used as a candidate gene for early-onset PE and its severe form and SGA complications.
authors.affiliation: Roche d.o.o. Serbia BU Diagnostics, Belgrade, Serbia.
authors.forename: Tijana
authors.initials: T
authors.lastname: Krnjeta
authors.affiliation: Clinic of Gynecology and Obstetrics, Clinical Center of Serbia, Belgrade, Serbia, and University of Belgrade - Faculty of Medicine, Belgrade, Serbia.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Mirković
authors.affiliation: Center for Medical Biochemistry, Clinical Center of Serbia, Belgrade, Serbia, and Department of Medical Biochemistry, University of Belgrade - Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ignjatović
authors.affiliation: Laboratory for Biochemistry and Molecular Diagnostics »Konzilijum«, Belgrade, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Tomašević
authors.affiliation: Laboratory for Biochemistry and Molecular Diagnostics »Konzilijum«, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Lukić
authors.affiliation: Laboratory for Biochemistry and Molecular Diagnostics »Konzilijum«, Belgrade, Serbia.
authors.forename: Drina
authors.initials: D
authors.lastname: Topalov
authors.affiliation: University of Belgrade - Faculty of Medicine, Belgrade, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Soldatović
authors.affiliation: Society of Medical Biochemistry of Serbia, Belgrade, Serbia.
authors.forename: Nada
authors.initials: N
authors.lastname: Majkić-Singh
keywords: COMT; SGA; early-onset preeclampsia; polymorphism; severe