title: Protective Effect of Cymbopogon citratus Essential Oil in Experimental Model of Acetaminophen-Induced Liver Injury.
abstract: To investigate the hepatoprotective effect of Cymbopogon citratus or lemongrass essential oil (LGO), it was used in an animal model of acute liver injury induced by acetaminophen (APAP). Swiss mice were pretreated with LGO (125, 250 and 500[Formula: see text]mg/kg) and SLM (standard drug, 200[Formula: see text]mg/kg) for a duration of seven days, followed by the induction of hepatotoxicity of APAP (single dose, 250[Formula: see text]mg/kg). The liver function markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase were determined to evaluate the hepatoprotective effects of the LGO. The livers were used to determine myeloperoxidase (MPO) activity, nitric oxide (NO) production and histological analysis. The effect of LGO on leukocyte migration was evaluated in vitro. Anti-oxidant activity was performed by assessing the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) in vitro. LGO pretreatment decreased significantly the levels of ALT, AST and ALP compared with APAP group. MPO activity and NO production were decreased. The histopathological analysis showed an improved of hepatic lesions in mice after LGO pretreatment. LGO inhibited neutrophil migration and exhibited anti-oxidant activity. Our results suggest that LGO has protective activity against liver toxicity induced by paracetamol.
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Nancy Sayuri
authors.initials: NS
authors.lastname: Uchida
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Saulo Euclides
authors.initials: SE
authors.lastname: Silva-Filho
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Rafael Pazinatto
authors.initials: RP
authors.lastname: Aguiar
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Luiz Alexandre Marques
authors.initials: LAM
authors.lastname: Wiirzler
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Gabriel Fernando Esteves
authors.initials: GFE
authors.lastname: Cardia
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Heitor Augusto Otaviano
authors.initials: HAO
authors.lastname: Cavalcante
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Francielli Maria de Souza
authors.initials: FMS
authors.lastname: Silva-Comar
authors.affiliation: † Department of Basic Health Science, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Tânia Cristina Alexandrino
authors.initials: TCA
authors.lastname: Becker
authors.affiliation: ‡ Department of Chemistry, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Expedito Leite
authors.initials: EL
authors.lastname: Silva
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Ciomar Aparecida
authors.initials: CA
authors.lastname: Bersani-Amado
authors.affiliation: * Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Roberto Kenji Nakamura
authors.initials: RKN
authors.lastname: Cuman
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects*; D018712:Analgesics, Non-Narcotic / Q000009:adverse effects*; D000818:Animals; D058633:Antipyretics / Q000009:adverse effects*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000209:etiology* / Q000517:prevention & control*; D031743:Cymbopogon / Q000737:chemistry*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy
chemical_list: D018712:Analgesics, Non-Narcotic; D058633:Antipyretics; D009822:Oils, Volatile; D000082:Acetaminophen
keywords: Acetaminophen; Acute Liver Injury; Cymbopogon citratus; Essential Oil; Hepatotoxicity; Lemongrass