title: Laccase - methacrylyol functionalized magnetic particles: Highly immobilized, reusable, and efficacious for methyl red decolourization.
abstract: Magnetically retrievable formulations of laccase potentially promising for biomedical and environmental applications were constructed by immobilization of the enzyme on the surface of magnetic nanoparticles functionalized by siloxane layers with active amino and methacrylyol moieties. The functional magnetic particles were prepared using a hydrolytic polycondensation reaction of tetraethoxysilane with 3-methacryloxypropyltrimethoxysilane (MPS). The results indicated that the amounts of laccase loading on the methacrylyol functional magnetic particles were approximately 150mg/g under the optimum conditions. The stability of the immobilized laccase was highly enhanced against thermal treatments and provided better long-term storage. Compared to free laccase, the immobilized laccase exhibited significantly higher decolourisation efficiency in the decolorization of a representative dye, methyl red, which resulted from the magnetic particles adsorption and enzymatic catalysis. These results show its great potential for industrial applications.
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Lab of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Qiujie
authors.initials: Q
authors.lastname: Lai
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yingju
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China. Electronic address: chens1968@hotmail.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Le
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D003116:Color; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006868:Hydrolysis; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism*; D008689:Methacrylates / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: C542237:3-methacryloxypropyltrimethoxysilane; D001391:Azo Compounds; D004800:Enzymes, Immobilized; D008689:Methacrylates; D012821:Silanes; C040733:tetraethoxysilane; C008492:methyl red; D012822:Silicon Dioxide; D042845:Laccase; D052203:Ferrosoferric Oxide
keywords: Decolorization; Functional magnetic particles; Immobilized laccase