title: Measuring Protein Interactions by Optical Biosensors.
abstract: This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is placed on description of robust approaches that will provide reliable results with few assumptions. How to avoid the most commonly encountered problems and artifacts is also discussed. © 2017 by John Wiley & Sons, Inc.
authors.affiliation: National Institutes of Health, Bethesda, Maryland.
authors.forename: Huaying
authors.initials: H
authors.lastname: Zhao
authors.affiliation: National Institutes of Health, Bethesda, Maryland.
authors.forename: Lisa F
authors.initials: LF
authors.lastname: Boyd
authors.affiliation: National Institutes of Health, Bethesda, Maryland.
authors.forename: Peter
authors.initials: P
authors.lastname: Schuck
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D007700:Kinetics; D015394:Molecular Structure; D055096:Optical Devices; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D015203:Reproducibility of Results; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D011506:Proteins
keywords: biolayer interferometry; optical biosensor; protein interactions; surface plasmon resonance