title: Effect of amino acids on the repression of alkaline protease synthesis in haloalkaliphilicNocardiopsis dassonvillei.
abstract: A newly isolated salt-tolerant alkaliphilic actinomycete,Nocardiopsis dassonvilleistrain OK-18 grows on mineral salts medium with glucose as carbon source. It also grows and produces protease with amino acids as sole carbon source. The synthesis of extracellular alkaline protease parallel to growth was repressible by substrate concentrations. The absolute production of the protease was delinked with growth under nutritional stress, as protease production was high, despite poor growth. When amino acids served as the sole source of carbon and nitrogen, the enzyme production was significantly controlled by the number of amino acids. Maximal protease production was achieved with proline, asparagine, tyrosine, alanine, methionine and valine as sole source of carbon and nitrogen in minimal medium. With the increasing number of different amino acids in the presence and absence of glucose, the protease production was synergistically lower as compared to complex medium.
authors.affiliation: UGC-CAS Department of Biosciences, Saurashtra University, Rajkot 360 005 Gujarat, India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Sharma
authors.affiliation: UGC-CAS Department of Biosciences, Saurashtra University, Rajkot 360 005 Gujarat, India.
authors.forename: Satya P
authors.initials: SP
authors.lastname: Singh
keywords: Alkaline protease; Enzyme repression; Halo-alkaliphiles; Marine actinobacteria; Nocardiopsis dassonvillei