title: Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells.
abstract: This study assessed RNA-binding motif 10 expression in lung adenocarcinoma tissues and examined the role and mechanism of RNA-binding motif 10 in the regulation of lung adenocarcinoma malignancy. Lung adenocarcinoma and corresponding adjacent non-tumor lung tissues from 41 patients were subjected to reverse transcription-polymerase chain reaction and Western blot assessment to detect RNA-binding motif 10 expression. Recombinant lentivirus carrying RNA-binding motif 10 complementary DNA was used to infect lung adenocarcinoma cell lines, A549 and H1299 cells. Complementary DNA microarray was used to profile RNA-binding motif 10-regulated genes. Levels of RNA-binding motif 10 messenger RNA and protein were significantly lower in lung adenocarcinoma tissues than those in paired non-tumor tissues (p < 0.001). Reduced RNA-binding motif 10 expression was found to be associated with an advanced tumor stage. RNA-binding motif 10 overexpression inhibited viability and colony formation capacity of lung adenocarcinoma cell lines and induced cell-cycle arrest at G0/G1 phase in A549 cells and at S phase in H1299 cells. Complementary DNA microarray analysis identified 304 upregulated and 386 downregulated genes induced by RNA-binding motif 10 overexpression, which may be involved in cancer, focal adhesion, peroxisome proliferator-activated receptor-regulated gene pathway, cytokine-cytokine receptor interaction, mitogen-activated protein kinase signaling, complement and coagulation cascades, platelet amyloid precursor protein pathway, extracellular matrix-receptor interaction, and small cell lung cancer-related genes. Expression of FGF2, EGFR, WNT5A, NF-κB, and RAP1A was downregulated, whereas expression of AKT2, BIRC3, and JUN was upregulated. RNA-binding motif 10 messenger RNA and protein were reduced in lung adenocarcinoma tissues, and RNA-binding motif 10 overexpression inhibited lung adenocarcinoma cancer cell malignant behavior in vitro. Molecularly, RNA-binding motif 10 regulates many gene pathways involving in the tumor development or progression.
authors.affiliation: 2 Department of Otolaryngology, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Guofang
authors.initials: G
authors.lastname: Guan
authors.affiliation: 3 Department of Urinary Surgery, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Ranwei
authors.initials: R
authors.lastname: Li
authors.affiliation: 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Tang
authors.affiliation: 4 Department of Anesthesiology, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Liu
authors.affiliation: 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Su
authors.affiliation: 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Tan
authors.affiliation: 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: 1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C512260:RBM10 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: RNA-binding motif 10; complementary DNA microarray; lung adenocarcinoma; tumor suppressor gene