title: Fecal Microbiota Transplantation in Patients With Blood Disorders Inhibits Gut Colonization With Antibiotic-Resistant Bacteria: Results of a Prospective, Single-Center Study.
abstract: Patients with blood disorders colonized with antibiotic-resistant bacteria (ARB) are prone to systemic infections that are difficult to treat. Reintroduction of commensal bacteria in a murine model of enterococcal colonization of the gut can lead to eradication of enterococci. We hypothesized that fecal microbiota transplantation (FMT) could be used to eradicate ARB in humans.
  
  
  
  Participants colonized with ARB were treated with intraduodenal FMT according to a prospective protocol (NCT02461199). The primary endpoint was complete ARB decolonization at 1 month after FMT. Secondary endpoints included safety assessment and partial ARB decolonization. Microbiome sequencing was performed to investigate the influence of microbial composition of the transplanted material on the outcome of FMT.
  
  
  
  Twenty-five FMTs were performed in 20 participants (including 40% who had neutropenia) who were colonized by a median of 2 (range, 1-4) strains of ARB. The primary endpoint was reached in 15/25 (60%) of the FMTs and more frequently in cases in which there was no periprocedural use of antibiotics (79% vs 36%, P < .05). Among participants, 15/20 (75%) experienced complete ARB decolonization. There were no severe adverse events, and partial ARB decolonization was observed in 20/25 (80%) of the FMTs. The microbiota composition analysis revealed higher abundance of Barnesiella spp., Bacteroides, and Butyricimonas and greater bacterial richness in the fecal material, resulting in eradication of Klebsiella pneumoniae compared with nonresponders.
  
  
  
  FMT in patients with blood disorders is safe and promotes eradication of ARB from the gastrointestinal tract.
  
  
  
  NCT02461199.
authors.affiliation: Department of Hematology, Oncology, and Internal Diseases, Medical University of Warsaw.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Bilinski
authors.affiliation: Foundation for the Infection Prevention Institute, Warsaw, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Grzesiowski
authors.affiliation: Clinical-Microbiomics, Copenhagen, Denmark.
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Sorensen
authors.affiliation: Department of Hematology, Oncology, and Internal Diseases, Medical University of Warsaw.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Madry
authors.affiliation: Department of Gastroenterology and Metabolic Diseases.
authors.forename: Jacek
authors.initials: J
authors.lastname: Muszynski
authors.affiliation: Department of Hematology, Oncology, and Internal Diseases, Medical University of Warsaw.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Robak
authors.affiliation: Department of Microbiology, Central Clinical Hospital.|Department of Dental Microbiology.
authors.forename: Marta
authors.initials: M
authors.lastname: Wroblewska
authors.affiliation: Department of Microbiology, Central Clinical Hospital.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Dzieciatkowski
authors.affiliation: Department of Epidemiology, Central Clinical Hospital, Medical University of Warsaw, Poland.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Dulny
authors.affiliation: Department of Hematology, Oncology, and Internal Diseases, Medical University of Warsaw.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Dwilewicz-Trojaczek
authors.affiliation: Department of Hematology, Oncology, and Internal Diseases, Medical University of Warsaw.
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Wiktor-Jedrzejczak
authors.affiliation: Department of Hematology, Oncology, and Internal Diseases, Medical University of Warsaw.
authors.forename: Grzegorz W
authors.initials: GW
authors.lastname: Basak
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D000069467:Fecal Microbiota Transplantation / Q000009:adverse effects / Q000706:statistics & numerical data; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D006402:Hematologic Diseases / Q000628:therapy*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents
keywords: antibiotic-resistant bacteria; fecal microbiota transplantation; gut colonization; hematology; infection