title: Evaluation of environmental and nutritional factors and sua gene on in vitro biofilm formation of Streptococcus uberis isolates.
abstract: The pathogenesis of Streptococcus uberis is attributed to a combination of extracellular factors and properties such as adherence and biofilm formation. The aim of this work was to evaluate the influence of different factors, additives and bovine milk compounds on S. uberis biofilm formation, as the presence of the sua gene by PCR. Additionally, extracellular DNA and the effect of DNaseI were evaluated in the biofilms yielded. Optimal biofilm development was observed when the pH was adjusted to 7.0 and 37 °C. Additives as glucose and lactose reduced biofilm formation as bovine milk compounds tested. PCR assay showed that not all the isolates yielded sua gene. Extrachromosomal ADN was found in cell-free supernatants, suggesting that DNA released spontaneously to the medium. The results contribute to a better understanding of the factors involved in biofilm production of this important pathogen associated with mastitis in order to promote the design of new therapeutic approaches.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina.
authors.forename: Melina Vanesa
authors.initials: MV
authors.lastname: Moliva
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Cerioli
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina. Electronic address: ereinoso@exa.unrc.edu.ar.
authors.forename: Elina Beatriz
authors.initials: EB
authors.lastname: Reinoso
mesh_terms: D000818:Animals; D001118:Argentina; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002364:Caseins / Q000494:pharmacology; D002417:Cattle; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003850:Deoxyribonuclease I / Q000494:pharmacology; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000494:pharmacology; D008413:Mastitis / Q000382:microbiology; D008414:Mastitis, Bovine / Q000382:microbiology; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002364:Caseins; D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012710:Serum Albumin, Bovine; C475729:casein hydrolysate; D003850:Deoxyribonuclease I; D005947:Glucose; D007785:Lactose
keywords: Biofilm; Mastitis; Streptococcus uberis