title: Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells.
abstract: In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.
authors.affiliation: Graduate School of Life and Environmental Sciences, The University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki, Japan. taka.noguchi@aist.go.jp.
authors.forename: Taka-Aki K
authors.initials: TK
authors.lastname: Noguchi
authors.affiliation: Graduate School of Life and Environmental Sciences, The University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki, Japan. akikuri@hotmail.com.|Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. akikuri@hotmail.com.
authors.forename: Akira
authors.initials: A
authors.lastname: Kurisaki
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009940:Organoids / Q000166:cytology* / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D013270:Stomach / Q000166:cytology*
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen
keywords: Barx1; Differentiation; Disease model; Dkk-1; Embryonic stem cells; Shh; Stomach organoids; Tissue formation