title: Constituents of the Roots of Dichapetalum pallidum and Their Anti-Proliferative Activity.
abstract: As part of our search for bioactive compounds from the Dichapetalaceae, repeated chromatographic purification of the roots of a hitherto unexamined species,Dichapetalum pallidum, led to the isolation of the newly occurring 7-hydroxydichapetalin P (1) and the known dichapetalins A (2) and X (3). Also isolated were the known compounds friedelin-2,3-lactone (4), friedelan-3-one (6), friedelan-3β-ol (7) and pomolic (8), as well as the dipeptide aurantiamide acetate (5). The compounds were characterized by direct interpretation of their IR, 1D NMR and 2D NMR spectral data and by comparison of their physico-chemical data, including their chromatographic profiles, with the literature and authentic samples in our compound library for the genusDichapetalum. The compounds were assayed for their anti-proliferative activities against the human T-lymphocytic leukemia (Jurkat), acute promyelocytic leukemia (HL-60) and T-lymphoblast-like leukemia (CEM) cell lines. Overall, dichapetalin X showed the strongest (3.14 μM) and broadest cytotoxic activities against all the leukemic cell lines tested, exhibiting even stronger activities than the standard compound, curcumin.
authors.affiliation: Chemistry Department, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 56, Legon, Accra, Ghana. dosei-safo@ug.edu.gh.
authors.forename: Dorcas
authors.initials: D
authors.lastname: Osei-Safo
authors.affiliation: Chemistry Department, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 56, Legon, Accra, Ghana. dzwgod001@myuct.ac.za.|Present address: Department of Chemistry, University of Cape Town, Rondebosch 7701, South Africa.. dzwgod001@myuct.ac.za.
authors.forename: Godwin Akpeko
authors.initials: GA
authors.lastname: Dziwornu
authors.affiliation: Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, P.O. Box LG 581, Legon, Accra, Ghana. antichama@yahoo.com.
authors.forename: Regina
authors.initials: R
authors.lastname: Appiah-Opong
authors.affiliation: Chemistry Department, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 56, Legon, Accra, Ghana. amewu@ug.edu.gh.
authors.forename: Mary Anti
authors.initials: MA
authors.lastname: Chama
authors.affiliation: Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, P.O. Box LG 581, Legon, Accra, Ghana. a-mensah@ug.edu.gh.
authors.forename: Isaac
authors.initials: I
authors.lastname: Tuffour
authors.affiliation: Department of Chemistry and Pharmacy, Friederich Alexander University of Erlangen-Nurnberg, Schuhstrasse 19, 91052 Erlangen, Germany. ropong@gmail.com.
authors.forename: Reiner
authors.initials: R
authors.lastname: Waibel
authors.affiliation: Chemistry Department, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 56, Legon, Accra, Ghana. ituffour@noguchi.ug.edu.gh.
authors.forename: Richard
authors.initials: R
authors.lastname: Amewu
authors.affiliation: Chemistry Department, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 56, Legon, Accra, Ghana. reiner.waibel@medchem.uni-erlangen.de.
authors.forename: Ivan
authors.initials: I
authors.lastname: Addae-Mensah
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D013141:Spiro Compounds / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; D013141:Spiro Compounds; C000613886:dichapetalin X
keywords: 7-hydroxydichapetalin P; Dichapetalum pallidum; dichapetalin A; dichapetalin X; leukemia; spiroketal