title: Gastroprotective potential of methanolic extract and dimethyl cardamonin from Campomanesia reitziana fruits in mice.
abstract: This study evaluated the gastroprotective potential of methanolic extract from fruits of Campomanesia reitziana (MECR) and its isolated chalcone dimethyl cardamonin (DMC). The phenolic compound in the extract, and the free radical scavenging activity of MECR and DMC, were quantified. The gastroprotective activity of MECR (30-300 mg/kg, p.o) and DMC (1 and 3 mg/kg, p.o) was determined by ethanol/HCl-induced gastric ulcers in mice. Histological, histochemical, and biochemical analyses were performed in the ulcerated tissue. MECR showed a high content of phenolic compounds, including DMC, and was able to scavenge DPPH radicals by 29.58% at 1000 μg/mL. However, DCM (1-1000 μg/mL) did not reduce DPPH radicals. Pre-treatment with MECR at doses of 100 and 300 mg/kg reduced the gastric lesions by 35.07 and 79.47%, respectively (ulcerated-vehicle group 10.72 ± 0.88 mm2). Moreover, the extract increased the mucin content by 1044.44% and superoxide dismutase activity by 20.04%, and decreased the lipoperoxide levels by 39.39%, compared to the ulcerated-vehicle group (0.27 ± 0.04 pixels × 103/field; 57.37 ± 1.59 U SOD/mg of protein and 29.57 ± 2.99 mmol LOOH/mg of tissue, respectively). However, MECR did not prevent the depletion of reduced glutathione or the decrease in catalase activity. Pre-treatment with DMC, at 1 and 3 mg/kg, also reduced the gastric ulcers by 53.83 and 52.64%, respectively. In summary, these findings confirm the gastroprotective activity of MECR and DMC, and open an interesting field concerning the gastroprotective potential of dimethyl cardamonin, given its potent antinociceptive and anti-inflammatory activity already described.
authors.affiliation: Graduação em Biomedicina, Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: de Oliveira Cabral
authors.affiliation: Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Campos
authors.affiliation: Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.|Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.
authors.forename: Luisa Mota
authors.initials: LM
authors.lastname: da Silva
authors.affiliation: Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.|Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.
authors.forename: Thaise
authors.initials: T
authors.lastname: Boeing
authors.affiliation: Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.|Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.
authors.forename: Sérgio Faloni
authors.initials: SF
authors.lastname: de Andrade
authors.affiliation: Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.|Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil.
authors.forename: Valdir Cechinel
authors.initials: VC
authors.lastname: Filho
authors.affiliation: Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí- UNIVALI, Itajaí, Brazil. nesello@univali.br.|Centro de Ciências da Saúde - CCS, Universidade do Vale do Itajaí - UNIVALI, Rua Uruguai, 458, Itajaí, Santa Catarina, 88302-202, Brazil. nesello@univali.br.
authors.forename: Luciane Ângela Nottar
authors.initials: LÂN
authors.lastname: Nesello
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D047188:Chalcones / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit; D000432:Methanol / Q000737:chemistry; D051379:Mice; D009077:Mucins / Q000378:metabolism; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D013276:Stomach Ulcer / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C501649:2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone; D000897:Anti-Ulcer Agents; D047188:Chalcones; D016166:Free Radical Scavengers; D009077:Mucins; D010936:Plant Extracts; D000431:Ethanol; D013482:Superoxide Dismutase; D000432:Methanol
keywords: Antioxidants; Campomanesia reitziana; Dimethyl cardamonin; Gastric ulcer; Myrtaceae