title: Functional Cryogel Microstructures Prepared by Light-Induced Cross-Linking of a Photoreactive Copolymer.
abstract: A novel, highly efficient method for the preparation of functional, microstructured and surface-attached cryogels is described. Photoinduced C,H-insertion reactions are used to generate cryogels in a single, rapid photo-cross-linking process. To this end, solutions containing both a photoreactive copolymer and the (bio)molecules to be immobilized are placed on a polymeric substrate followed by freezing and a short UV exposure. This strategy combines photolithography and cryogel formation allowing for a simultaneous generation and (bio)functionalization of cryogels in a single reaction step. To demonstrate the potential of the generated materials for bioanalytical applications, we successfully prepared DNA and protein cryogel microarrays.
authors.affiliation: Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg , Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
authors.forename: Marc
authors.identifier: http://orcid.org/0000-0003-4926-9791
authors.initials: M
authors.lastname: Zinggeler
authors.affiliation: Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg , Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
authors.forename: Jan-Niklas
authors.initials: JN
authors.lastname: Schönberg
authors.affiliation: Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg , Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
authors.forename: Patrick L
authors.initials: PL
authors.lastname: Fosso
authors.affiliation: Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg , Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brandstetter
authors.affiliation: Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg , Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rühe
keywords: cryogel microstructure(s); functional cryogel(s); photo-cross-linking; porous hydrogel(s); surface-attached cryogel(s)