title: Dysregulation of Placental miRNA in Maternal Obesity Is Associated With Pre- and Postnatal Growth.
abstract: Human placenta exhibits a specific microRNA (miRNA) expression pattern. Some of these miRNAs are dysregulated in pregnancy disorders such as preeclampsia and intrauterine growth restriction and are potential biomarkers for these pathologies.
  
  
  
  To study the placental miRNA profile in pregnant women with pregestational overweight/obesity (preOB) or gestational obesity (gestOB) and explore the associations between placental miRNAs dysregulated in maternal obesity and prenatal and postnatal growth.
  
  
  
  TaqMan Low Density Arrays and real-time polymerase chain reaction were used to profile the placental miRNAs in 70 pregnant women (20 preOB, 25 gestOB, and 25 control). Placentas and newborns were weighed at delivery, and infants were weighed at 1, 4, and 12 months of age.
  
  
  
  Eight miRNAs were decreased in placentas from preOB or gestOB (miR-100, miR-1269, miR-1285, miR-181, miR-185, miR-214, miR-296, and miR-487) (all P < 0.05). Among them, miR-100, miR-1285, miR-296, and miR-487 were associated with maternal metabolic parameters (all P < 0.05) and were predictors of lower birth weight (all P < 0.05; R2 > 30%) and increased postnatal weight gain (all P < 0.05; R2 > 20%). In silico analysis showed that these miRNAs were related to cell proliferation and insulin signaling pathways. miR-296 was also present in plasma samples and associated with placental expression and prenatal and postnatal growth parameters (all P < 0.05).
  
  
  
  We identified a specific placental miRNA profile in maternal obesity. Placental miRNAs dysregulated in maternal obesity may be involved in mediation of growth-promoting effects of maternal obesity on offspring and could be used as early markers of prenatal and postnatal growth.
authors.affiliation: Pediatric Endocrinology Group, Girona Biomedical Research Institute (IDIBGI), Dr. Trueta University Hospital, Girona 17007, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Carreras-Badosa
authors.affiliation: Department of Gynecology, Dr. Trueta University Hospital, Girona 17007, Spain.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bonmatí
authors.affiliation: Diabetes, Endocrinology and Nutrition Group, Girona Biomedical Research Institute (IDIBGI), Dr. Trueta University Hospital, Centro de Investigación Biomédica en Red-Fisiopatología de la Obesidad y Nutrición (CIBERobn), Girona 17007, Spain.
authors.forename: Francisco-Jose
authors.initials: FJ
authors.lastname: Ortega
authors.affiliation: Joint Barcelona Supercomputing Center, Centre for Genomic Regulation, Institute for Research in Biomedicine (BSC-CRG-IRB) Research Program in Computational Biology, Barcelona Supercomputing Center, Barcelona 08028, Spain.
authors.forename: Josep-Maria
authors.initials: JM
authors.lastname: Mercader
authors.affiliation: Joint Barcelona Supercomputing Center, Centre for Genomic Regulation, Institute for Research in Biomedicine (BSC-CRG-IRB) Research Program in Computational Biology, Barcelona Supercomputing Center, Barcelona 08028, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Guindo-Martínez
authors.affiliation: Joint Barcelona Supercomputing Center, Centre for Genomic Regulation, Institute for Research in Biomedicine (BSC-CRG-IRB) Research Program in Computational Biology, Barcelona Supercomputing Center, Barcelona 08028, Spain.|Institució Catalana de Recerca i Estudis Avançats, 08010 Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Torrents
authors.affiliation: Department of Physical Therapy, Escola Universitària de la Salut i l'Esport, University of Girona, 17007 Girona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Prats-Puig
authors.affiliation: Institute of Legal Medicine of Catalonia, 17001 Girona, Spain.
authors.forename: Jose-Maria
authors.initials: JM
authors.lastname: Martinez-Calcerrada
authors.affiliation: Department of Development and Regeneration, University of Leuven, 3000 Leuven, Belgium.
authors.forename: Francis
authors.initials: F
authors.lastname: de Zegher
authors.affiliation: Endocrinology, Hospital Sant Joan de Déu, University of Barcelona, 08950 Esplugues, Barcelona.|Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III (ISCIII), 28220 Madrid, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Ibáñez
authors.affiliation: Diabetes, Endocrinology and Nutrition Group, Girona Biomedical Research Institute (IDIBGI), Dr. Trueta University Hospital, Centro de Investigación Biomédica en Red-Fisiopatología de la Obesidad y Nutrición (CIBERobn), Girona 17007, Spain.
authors.forename: Jose-Manuel
authors.initials: JM
authors.lastname: Fernandez-Real
authors.affiliation: Pediatric Endocrinology Group, Girona Biomedical Research Institute (IDIBGI), Dr. Trueta University Hospital, Girona 17007, Spain.
authors.forename: Abel
authors.initials: A
authors.lastname: Lopez-Bermejo
authors.affiliation: Pediatric Endocrinology Group, Girona Biomedical Research Institute (IDIBGI), Dr. Trueta University Hospital, Girona 17007, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Bassols
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002657:Child Development / Q000502:physiology; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D035683:MicroRNAs / Q000378:metabolism*; D009765:Obesity / Q000175:diagnosis*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis / Q000378:metabolism*; D011296:Prenatal Diagnosis / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity; D015430:Weight Gain / Q000502:physiology
chemical_list: D015415:Biomarkers; D035683:MicroRNAs