title: A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione.
abstract: The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm).
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133-002, China.
authors.forename: Zhixue
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133-002, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133-002, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea.
authors.forename: Nahyun
authors.initials: N
authors.lastname: Kwon
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133-002, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea.
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D005978:Glutathione
keywords: antioxidants; fluorescent probes; gluthionine; ratiometric probes; redox homeostasis