title: The convergent chemical synthesis of histone H3 protein for site-specific acetylation at Lys56 and ubiquitination at Lys122.
abstract: Deposition of (H3-H4)2tetramers is believed to be the critical step in nucleosome assembly. Site-specific acetylation and ubiquitination of histone H3 have been speculated to synergistically facilitate the formation and deposition of (H3-H4)2tetramers. Here we report our endeavors toward the first chemical synthesis of homogenous histone H3, which bears Lys56 acetylation and Lys122 ubiquitination, for in vitro biochemical and biophysical studies.
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084, China and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. lijiabin@ustc.edu.cn.
authors.forename: Yun-Kun
authors.identifier: http://orcid.org/0000-0002-0015-5964
authors.initials: YK
authors.lastname: Qi
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Qiao-Qiao
authors.initials: QQ
authors.lastname: He
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Hua-Song
authors.initials: HS
authors.lastname: Ai
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Tsinghua-Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084, China and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. lijiabin@ustc.edu.cn.
authors.forename: Jia-Bin
authors.identifier: http://orcid.org/0000-0002-5308-5787
authors.initials: JB
authors.lastname: Li
mesh_terms: D000107:Acetylation; D006657:Histones / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D006657:Histones; D008239:Lysine