title: Strain improvement of industrially important microorganisms based on resistance to toxic metabolites and abiotic stress.
abstract: Improvement of the biosynthetic capabilities of industrially relevant microbes to produce desired metabolites in higher quantities is one of the important topics of modern biotechnology. In this article, different strategies of improvement of mutated microbial strains are briefly described. This is followed by the first comprehensive review of the literature on obtaining high yielding microorganisms, that is, mutants exhibiting resistance to antimetabolites, nutritional repression, and abiotic stresses as well as tolerance to solvents and toxic substrates or products. Furthermore, the efficiency of the microbial metabolites produced by improved microbial strains, advantages, and limitations, as well as future prospects for strategies of strain development are discussed.
authors.affiliation: Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Jan
authors.identifier: http://orcid.org/0000-0002-2977-1269
authors.initials: J
authors.lastname: Fiedurek
authors.affiliation: Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Trytek
authors.affiliation: Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Szczodrak
mesh_terms: D000963:Antimetabolites / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology; D004361:Drug Tolerance; D005285:Fermentation; D007218:Industrial Microbiology; D053858:Metabolic Networks and Pathways; D009154:Mutation; D012997:Solvents / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D000963:Antimetabolites; D012997:Solvents
keywords: abiotic stresses; mutants; strain improvement; stress-tolerance; toxic metabolites