title: Species identification of white false hellebore (Veratrum album subsp. oxysepalum) using real-time PCR.
abstract: Food poisoning is frequently caused by the accidental ingestion of toxic plants that possess strong morphological similarities to edible plants. False helleborine (Veratrum album) is one of the most common plants involved in such accidents. In cases of poisoning by toxic plants, rapid and accurate identification, usually based on the morphological or chemical analysis of plant parts, is required for appropriate medical treatment or forensic investigation. However, morphological examinations require experience in systematic botany because the samples are fragmentary, and chemical analysis of natural compounds can be difficult. In this study, we developed a TaqMan real-time PCR method using trnH-psbA and trnL-trnF that could be carried out in 30-60min. The lower detection limit was less than 10pg of DNA and the primer sets were specific to V. album and Veratrum stamineum. Mixed samples, cooked samples, and simulated gastric contents were successfully identified, and a multiplex assay of two regions was also possible. These results indicate that the TaqMan real-time PCR analysis is a very effective method to detect small samples of V. album and V. stamineum accurately and rapidly in poisoning cases.
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. Electronic address: kikkawa@nrips.go.jp.
authors.forename: Hitomi S
authors.initials: HS
authors.lastname: Kikkawa
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Kouichiro
authors.initials: K
authors.lastname: Tsuge
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.affiliation: Medical Plant Garden, Tokyo Metropolitan Institute of Public Health, 21-1 Nakajimacho, Kodaira, Tokyo 187-0033, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Aragane
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Hikoto
authors.initials: H
authors.lastname: Ohta
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Sugita
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D005517:Foodborne Diseases; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D010947:Plants, Toxic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014703:Veratrum / Q000235:genetics*
chemical_list: D018744:DNA, Plant
keywords: DNA barcoding; Forensic science; TaqMan real-time PCR; Veratrum album; Veratrum stamineum