title: Drosophila Male Meiosis.
abstract: In Drosophila males, there is no synaptonemal complex and recombination does not occur. Thus, Drosophila male meiosis is a good model system for the analysis of achiasmate chromosome segregation. In addition, due to their large size, the meiotic spindles of Drosophila males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live Drosophila testes and for preparation of fixed meiotic chromosomes and spindles.
authors.affiliation: Department of Biology and Biotechnology "Charles Darwin", Sapienza, University of Rome, P.le. A Moro 5, 00185, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bonaccorsi
authors.affiliation: Department of Biology and Biotechnology "Charles Darwin", Sapienza, University of Rome, P.le. A Moro 5, 00185, Rome, Italy. maurizio.gatti@uniroma1.it.|Institute of Molecular Biology and Pathology (IBPM) of CNR, Rome, Italy. maurizio.gatti@uniroma1.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gatti
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods; D004330:Drosophila / Q000166:cytology* / Q000235:genetics; D008297:Male; D008540:Meiosis; D008941:Spindle Apparatus; D013737:Testis / Q000166:cytology*
keywords: Chromosomes; Drosophila; Immunostaining; Live analysis; Male meiosis; Meiotic spindle; Spermatogenesis