title: Production of mycophenolic acid byPenicillium brevicompactum-A comparison of two methods of optimization.
abstract: Production of mycophenolic acid (MPA) by submerged fermentation using the microfungusPenicillium brevicompactumMTCC 8010 is reported here. Screening experiments were used to identify: the suitable media composition; the optimal initial pH; and the optimal incubation temperature to maximize the production of MPA in batch cultures. The initial concentrations of the selected sources of carbon (glucose), nitrogen (peptone) and the precursors (methionine, glycine) were then optimized by: (1) one-at-a-time variation of factors; and (2) a central composite design (CCD) of experiments, in a 12-day batch culture at an initial pH of 5.0, an incubation temperature of 25 °C, and an agitation speed of 200 rpm. The medium optimized using the one-at-a-time variation yielded a peak MPA titer of 1232 ± 90 mg/L. The medium optimized by the CCD method yielded a 40% higher MPA titer of 1737 ± 55 mg/L. The latter value was nearly 9-fold greater than the titer achieved prior to optimization.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Patel
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India.
authors.forename: Mahesh D
authors.initials: MD
authors.lastname: Patil
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India.
authors.forename: Surbhi
authors.initials: S
authors.lastname: Soni
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India.
authors.forename: Taresh P
authors.initials: TP
authors.lastname: Khobragade
authors.affiliation: School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Chisti
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062, Punjab, India.
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
keywords: CCD; Fermentation optimization; Immunosuppressant; Mycophenolic acid; Penicillium brevicompactum