title: Simultaneous Determination and Pharmacokinetic Study of Quercetin, Luteolin, and Apigenin in Rat Plasma after Oral Administration ofMatricaria chamomillaL. Extract by HPLC-UV.
abstract: A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration ofMatricaria chamomillaL. extract. The flow rate was set at 1.0 ml/min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11-11.36μg/ml for quercetin, 0.11-11.20μg/ml for luteolin, and 0.11-10.60μg/ml for apigenin, respectively. The intraday and interday precisions (RSD) were less than 8.32 and 8.81%, respectively. The lower limits of quantification (LLOQ) of the three compounds were 0.11μg/ml. The mean recoveries for quercetin, luteolin, and apigenin were 99.11, 95.62, and 95.21%, respectively. Stability studies demonstrated that the three compounds were stable in the preparation and analytical process. The maximum plasma concentration (Cmax) was 0.29 ± 0.06, 3.04 ± 0.60, and 0.42 ± 0.10μg/ml, respectively. The time to reach the maximum plasma concentration (Tmax) was 0.79 ± 0.25, 0.42 ± 0.09, and 0.51 ± 0.13 h, respectively. The validated method was successfully applied to investigate the pharmacokinetics study of quercetin, luteolin, and apigenin in rat plasma after oral administration ofM. chamomillaextract.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xiaoxv
authors.initials: X
authors.lastname: Dong
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China; Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lan
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yin
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Chunjing
authors.initials: C
authors.lastname: Yang
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Jian
authors.identifier: 0000-0003-1974-044X
authors.initials: J
authors.lastname: Ni