title: Regulation of plant immune receptor accumulation through translational repression by a glycine-tyrosine-phenylalanine (GYF) domain protein.
abstract: Plant immunity is tightly regulated to ensure proper defense against surrounding microbial pathogens without triggering autoimmunity, which negatively impacts plant growth and development. Immune receptor levels are intricately controlled by RNA processing and post-translational modification events, such as ubiquitination. It remains unknown whether, and if yes, how, plant immune receptor homeostasis is regulated at the translational level. From amutant,snc1-enhancing(muse) forward genetic screen, we identified MUSE11/EXA1, which negatively regulates nucleotide-binding leucine-rich repeat (NLR) receptor mediated defence. EXA1 contains an evolutionarily conserved glycine-tyrosine-phenylalanine (GYF) domain that binds proline-rich sequences. Genetic and biochemical analysis revealed that loss ofEXA1leads to heightened NLR accumulation and enhanced resistance against virulent pathogens. EXA1 also associates with eIF4E initiation factors and the ribosome complex, likely contributing to the proper translation of target proteins. In summary, our study reveals a previously unknown mechanism of regulating NLR homeostasis through translational repression by a GYF protein.
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, Canada.|Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: Zhongshou
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, Canada.|Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: Shuai
authors.initials: S
authors.lastname: Huang
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, Canada.|Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, China.
authors.forename: Shitou
authors.initials: S
authors.lastname: Xia
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, Canada.|Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: Xin
authors.identifier: 0000-0002-6354-2021
authors.initials: X
authors.lastname: Li
mesh_terms: D017360:Arabidopsis; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000378:metabolism*; D014176:Protein Biosynthesis; D011971:Receptors, Immunologic / Q000378:metabolism*; D014026:Nicotiana
chemical_list: D010940:Plant Proteins; D011971:Receptors, Immunologic
keywords: A. thaliana; NLR receptor; plant biology; plant immunity; resistance protein homeostasis; translational control