title: Low-cost, rapidly-developed, 3D printedin vitrocorpus callosum model for mucopolysaccharidosis type I.
abstract: The rising prevalence of high throughput screening and the general inability of (1) two dimensional (2D) cell culture and (2)in vitrorelease studies to predictin vivoneurobiological and pharmacokinetic responses in humans has led to greater interest in more realistic three dimensional (3D) benchtop platforms. Advantages of 3D human cell culture over its 2D analogue, or even animal models, include taking the effects of microgeometry and long-range topological features into consideration. In the era of personalized medicine, it has become increasingly valuable to screen candidate molecules and synergistic therapeutics at a patient-specific level, in particular for diseases that manifest in highly variable ways. The lack of established standards and the relatively arbitrary choice of probing conditions has limitedin vitrodrug release to a largely qualitative assessment as opposed to a predictive, quantitative measure of pharmacokinetics and pharmacodynamics in tissue. Here we report the methods used in the rapid, low-cost development of a 3D model of a mucopolysaccharidosis type I patient's corpus callosum, which may be used for cell culture and drug release. The CAD model is developed fromin vivobrain MRI tracing of the corpus callosum using open-source software, printed with poly (lactic-acid) on a Makerbot Replicator 5X, UV-sterilized, and coated with poly (lysine) for cellular adhesion. Adaptations of material and 3D printer for expanded applications are also discussed.
authors.affiliation: CoCreateX, Inc., St. Paul, USA; Precision Horizons, Minneapolis, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Tabet
authors.affiliation: CoCreateX, Inc., St. Paul, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Gardner
authors.affiliation: CoCreateX, Inc., St. Paul, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Swanson
authors.affiliation: Precision Horizons, Minneapolis, USA.
authors.forename: Sydney
authors.initials: S
authors.lastname: Crump
authors.affiliation: CoCreateX, Inc., St. Paul, USA.
authors.forename: Austin
authors.initials: A
authors.lastname: McMeekin
authors.affiliation: CoCreateX, Inc., St. Paul, USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Gong
authors.affiliation: CoCreateX, Inc., St. Paul, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Tabet
authors.affiliation: CoCreateX, Inc., St. Paul, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hacker
authors.affiliation: Department of Pediatrics, University of Minnesota, Minneapolis, USA.
authors.forename: Igor
authors.initials: I
authors.lastname: Nestrasil
keywords: 3D printing; cell culture; corpus callosum; in vitro release; mucopolysaccharidosis; neurodegenerative disease