title: Serotonin-induced hypophagia is mediated via α2and β2adrenergic receptors in neonatal layer-type chickens.
abstract: 1. Serotoninergic and adrenergic systems play crucial roles in feed intake regulation in avians but there is no report on possible interactions among them. So, in this study, 5 experiments were designed to evaluate the interaction of central serotonergic and adrenergic systems on food intake regulation in 3 h food deprived (FD3) neonatal layer-type chickens. 2. In Experiment 1, chickens received intracerebroventricular (ICV) injection of control solution, serotonin (56.74 nmol), prazosin (α1receptor antagonist, 10 nmol) and co-injection of serotonin plus prazosin. In Experiment 2, control solution, serotonin (56.74 nmol), yohimbine (α2receptor antagonist, 13 nmol) and co-injection of serotonin plus yohimbine were used. In Experiment 3, the birds received control solution, serotonin (56.74 nmol), metoprolol (β1receptor antagonist, 24 nmol) and co-injection of serotonin plus metoprolol. In Experiment 4, injections were control solution, serotonin (56.74 nmol), ICI 118.551 (β2receptor antagonist, 5 nmol) and serotonin plus ICI 118.551. In Experiment 5, control solution, serotonin (56.74 nmol), SR59230R (β3receptor antagonist, 20 nmol) and co-administration of serotonin and SR59230R were injected. In all experiments the cumulative food intake was measured until 120 min post injection. 3. The results showed that ICV injection of serotonin alone decreased food intake in chickens. A combined injection of serotonin plus ICI 118.551 significantly attenuated serotonin-induced hypophagia. Also, co-administration of serotonin and yohimbine significantly amplified the hypophagic effect of serotonin. However, prazosin, metoprolol and SR59230R had no effect on serotonin-induced hypophagia in chickens. 4. These results suggest that serotonin-induced feeding behaviour is probably mediated via α2and β2adrenergic receptors in neonatal layer-type chicken.
authors.affiliation: a Department of Basic Sciences, Faculty of Veterinary Medicine , University of Tehran , Tehran , Iran.
authors.forename: M
authors.initials: M
authors.lastname: Zendehdel
authors.affiliation: b Department of Physiology, Biology Research Center, Zanjan Branch , Islamic Azad University , Zanjan , Iran.
authors.forename: F
authors.initials: F
authors.lastname: Sardari
authors.affiliation: c Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: S
authors.initials: S
authors.lastname: Hassanpour
authors.affiliation: b Department of Physiology, Biology Research Center, Zanjan Branch , Islamic Azad University , Zanjan , Iran.
authors.forename: M
authors.initials: M
authors.lastname: Rahnema
authors.affiliation: c Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: A
authors.initials: A
authors.lastname: Adeli
authors.affiliation: b Department of Physiology, Biology Research Center, Zanjan Branch , Islamic Azad University , Zanjan , Iran.
authors.forename: E
authors.initials: E
authors.lastname: Ghashghayi
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D004435:Eating / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects*; D005260:Female; D005508:Food Deprivation; D057967:Infusions, Intraventricular; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism*; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*; D012701:Serotonin / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D018341:Receptors, Adrenergic, alpha-2; D018343:Receptors, Adrenergic, beta-2; D012701:Serotonin
keywords: Serotonergic; adrenergic; food intake; layer-type chicken