title: Transient Cerebral Ischemia Alters GSK-3β and p-GSK-3β Immunoreactivity in Pyramidal Neurons and Induces p-GSK-3β Expression in Astrocytes in the Gerbil Hippocampal CA1 Area.
abstract: Glycogen synthase kinase 3β (GSK-3β) is a key downstream protein in the PI3K/Akt pathway. Phosphorylation of serine 9 of GSK-3β (GSK-3β activity inhibition) promotes cell survival. In this study, we examined changes in expressions of GSK-3β and phosphorylation of GSK-3β (p-GSK-3β) in the gerbil hippocampal CA1 area after 5 min of transient cerebral ischemia. GSK-3β immunoreactivity in the CA1 area was increased in pyramidal cells at 6 h after ischemia-reperfusion. It was decreased in CA1 pyramidal cells from 12 h after ischemia-reperfusion, and hardly detected in the CA1 pyramidal cells at 5 days after ischemia-reperfusion. p-GSK-3β immunoreactivity was slightly decreased in CA1 pyramidal cells at 6 and 12 h after ischemia-reperfusion. It was significantly increased in these cells at 1 and 2 days after ischemia-reperfusion. Five days after ischemia-reperfusion, p-GSK-3β immunoreactivity was hardly found in CA1 pyramidal cells. However, p-GSK-3β immunoreactivity was strongly expressed in astrocytes primarily distributed in strata oriens and radiatum. In conclusion, GSK-3β and p-GSK-3β were significantly changed in pyramidal cells and/or astrocytes in the gerbil hippocampal CA1 area following 5 min of transient cerebral ischemia. This finding indicates that GSK-3β and p-GSK-3β are closely related to delayed neuronal death.
authors.affiliation: Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, People's Republic of China.
authors.forename: Bai Hui
authors.initials: BH
authors.lastname: Chen
authors.affiliation: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, 24252, South Korea.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, 24252, South Korea.
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, 24252, South Korea.
authors.forename: Bich Na
authors.initials: BN
authors.lastname: Shin
authors.affiliation: Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, 24252, South Korea.
authors.forename: Yun Lyul
authors.initials: YL
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, Hallym University, Chuncheon, 24252, South Korea.
authors.forename: Il Jun
authors.initials: IJ
authors.lastname: Kang
authors.affiliation: Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: Seongkweon
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Radiology, School of Medicine, Kangwon National University, Chuncheon, 24289, South Korea.
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Jeon
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: Jeong Hwi
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: Tae-Kyeong
authors.initials: TK
authors.lastname: Lee
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: Jae Chul
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea. mhwon@kangwon.ac.kr.
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.affiliation: Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea.
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea. zammanza@kangwon.ac.kr.
authors.forename: Joong Bum
authors.initials: JB
authors.lastname: Moon
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000201:enzymology* / Q000473:pathology; D001362:Avoidance Learning / Q000502:physiology; D002545:Brain Ischemia / Q000201:enzymology* / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000737:chemistry / Q000201:enzymology* / Q000473:pathology; D016923:Cell Death / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D005849:Gerbillinae; D000071679:Glycogen Synthase Kinase 3 beta / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D017966:Pyramidal Cells / Q000737:chemistry / Q000201:enzymology* / Q000473:pathology
chemical_list: D000071679:Glycogen Synthase Kinase 3 beta
keywords: Astrocyte; Glycogen synthase kinase; Hippocampal CA1 area; Ischemia–reperfusion injury; Phosphoinositide-3-kinase/Akt pathway; Pyramidal cell