title: Luminescent Lanthanide-Based Organic/Inorganic Hybrid Materials for Discrimination of Glutathione in Solution and within Hydrogels.
abstract: Glutathione (GSH) as a biothiol is an essential peptide related to various diseases. Although multiple strategies for biothiols detection have been developed, there is increasing demand for sensors that can differentiate GSH from cysteine (Cys) and homocysteine (Hcy), owing to the similar structures and thiol groups in these amino acids. Herein, we report a novel Eu3+/LAPONITE (Lap)-based organic/inorganic hybrid material for selective detection of GSH via an "off-on" process. The fluorescence of Eu(DPA)3@Lap-Tris can be quenched by Cu2+through photoinduced electron transfer (PET). The addition of GSH into the Eu(DPA)3@Lap-Tris/Cu2+system induces the removal of Cu2+from Eu(DPA)3@Lap-Tris and blocks PET, resulting in the recovery of fluorescence. This proposed assay demonstrates higher selectivity toward GSH than Cys and Hcy, and showed a detection limit of 162 nM within a linear range of 0.5-30 μM. Unlike other GSH selective sensors, this platform could be formed into a hydrogel while its sensitivity was maintained. The sensitive response to GSH in serum samples makes this platform an efficient tool for biological applications because of its ease of preparation, high selectivity, good biocompatibility, and low toxicity.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Chai
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
authors.forename: Huanrong
authors.identifier: http://orcid.org/0000-0001-7400-7668
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology , Guangrong Dao No.8, Hongqiao District, Tianjin 300130, China.
authors.forename: Binyuan
authors.initials: B
authors.lastname: Liu
keywords: detection; discrimination; glutathione; hydrogel; nanoclay