title: Multi-Förster Resonance Energy Transfer-Based Fluorescent Probe for Spatiotemporal Matrix Metalloproteinase-2 and Caspase-3 Imaging.
abstract: A novel single-molecular fluorescent probe was developed for spatiotemporal matrix metalloproteinase-2 (MMP-2) and caspase-3 imaging with distinct fluorescence signals. Due to the multi-Förster resonance energy transfer (FRET) processes, the probe could respond to MMP-2 and caspase-3 independently with high signal-to-noise ratio. Moreover, the overexpression of MMP-2 in cancer cell lines and the cisplatin induced cell apoptosis were spatiotemporal imaged with distinct fluorescence emissions. Because of the independent process of the probe for MMP-2 and caspase-3 imaging, the probe could meet the demands for precise disease diagnosis and cancer theranostic applications, which could extensively simplify the processes for precise cancer diagnosis and imaging.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Li
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Hao-Ran
authors.initials: HR
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Chu-Xin
authors.initials: CX
authors.lastname: Li
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Bo-Ru
authors.initials: BR
authors.lastname: Xie
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Ke-Wei
authors.initials: KW
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, The Institute for Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors.forename: Xian-Zheng
authors.identifier: 0000-0001-6242-6005
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019556:COS Cells; D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry / Q000378:metabolism*; D018613:Microscopy, Confocal
chemical_list: D005456:Fluorescent Dyes; D053148:Caspase 3; D020778:Matrix Metalloproteinase 2