title: Ergosteryl 2-naphthoate, An Ergosterol Derivative, Exhibits Antidepressant Effects Mediated by the Modification of GABAergic and Glutamatergic Systems.
abstract: Phytosterols are a kind of natural component including sitosterol, campesterol, avenasterol, ergosterol (Er) and others. Their main natural sources are vegetable oils and their processed products, followed by grains, by-products of cereals and nuts, and small amounts of fruits, vegetables and mushrooms. In this study, three new Er monoester derivatives were obtained from the reflux reaction with Er: organic acids (furoic acid, salicylic acid and 2-naphthoic acid), 1-Ethylethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride (EDCI) and 4-dimethylaminopyridine (DMAP) in dichloromethane. Their chemical structures were defined by IR and NMR. The present study was also undertaken to investigate the antidepressant-like effects of Er and its derivatives in male adult mice models of depression, and their probable involvement of GABAergic and glutamatergic systems by the forced swim test (FST). The results indicated that Er and its derivatives display antidepressant effects. Moreover, one derivative of Er, ergosteryl 2-naphthoate (ErN), exhibited stronger antidepressant activity in vivo compared to Er. Acute administration of ErN (5 mg/kg, i.p.) and a combination of ErN (0.5 mg/kg, i.p.), reboxetine (2.5 mg/kg, i.p.), and tianeptine (15 mg/kg, i.p.) reduced the immobility time in the FST. Pretreatment with bicuculline (a competitive γ-aminobutyric acid (GABA) antagonist, 4 mg/kg, i.p.) andN-methyl-d-aspartic acid (NMDA, an agonist at the glutamate site, 75 mg/kg, i.p.) effectively reversed the antidepressant-like effect of ErN (5 mg/kg, i.p.). However, prazosin (a α1-adrenoceptor antagonist, 1 mg/kg, i.p.) and haloperidol (a non-selective D2 receptor antagonist, 0.2 mg/kg, i.p.) did not eliminate the reduced immobility time. Altogether, these results indicated that ErN produced antidepressant-like activity, which might be mediated by GABAergic and glutamatergic systems.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. 18037179167@163.com.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Lin
authors.affiliation: Institute of Translational Medicine, the First Hospital of Jilin University, Xinmin Street 71th, Changchun 130021, Jilin, China. hjli2012@jlu.edu.cn.
authors.forename: Haijun
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. zhaoyan@jlau.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. 18339901371@163.com.
authors.forename: Enbo
authors.initials: E
authors.lastname: Cai
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. popzhy@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. gaoyugang_2006@163.com.
authors.forename: Yugang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. Liu-shuangli@163.com.
authors.forename: Shuangli
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. 15543098032@163.com.
authors.forename: He
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. zlxbooksea@163.com.
authors.forename: Lianxue
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888th, Changchun 130118, Jilin, China. tangguosheng0113@126.com.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Tang
authors.affiliation: Center of Ophthalmology, the Second Hospital of Jilin University, Ziqiang Street 218th, Changchun 130041, Jilin, China. m15038326367@163.com.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D001640:Bicuculline / Q000494:pharmacology; D002490:Central Nervous System / Q000187:drug effects / Q000473:pathology; D003863:Depression / Q000188:drug therapy; D004298:Dopamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004875:Ergosterol / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D005971:Glutamates / Q000378:metabolism*; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D016202:N-Methylaspartate / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D013550:Swimming; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D000928:Antidepressive Agents; D005971:Glutamates; C000619277:ergosteryl 2-naphthoate; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D004298:Dopamine; D011224:Prazosin; D001640:Bicuculline; D004875:Ergosterol
keywords: antidepressant; ergosterol; ergosteryl 2-naphthoate; forced swim test