title: How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers.
abstract: This review presents data on the influence of various experimental parameters on the binding of proteins onto Langmuir lipid monolayers. The users of the Langmuir methodology are often unaware of the importance of choosing appropriate experimental conditions to validate the data acquired with this method. The protein Retinitis pigmentosa 2 (RP2) has been used throughout this review to illustrate the influence of these experimental parameters on the data gathered with Langmuir monolayers. The methods detailed in this review include the determination of protein binding parameters from the measurement of adsorption isotherms, infrared spectra of the protein in solution and in monolayers, ellipsometric isotherms and fluorescence micrographs.
authors.affiliation: CUO-Recherche, Hôpital du Saint-Sacrement, Centre de recherche du CHU de Québec and Département d'ophtalmologie, Faculté de médecine, and Regroupement stratégique PROTEO, Université Laval, Québec, Québec, Canada. Electronic address: Elodie.Boisselier@fmed.ulaval.ca.
authors.forename: Élodie
authors.initials: É
authors.lastname: Boisselier
authors.affiliation: CUO-Recherche, Hôpital du Saint-Sacrement, Centre de recherche du CHU de Québec and Département d'ophtalmologie, Faculté de médecine, and Regroupement stratégique PROTEO, Université Laval, Québec, Québec, Canada.
authors.forename: Éric
authors.initials: É
authors.lastname: Demers
authors.affiliation: CUO-Recherche, Hôpital du Saint-Sacrement, Centre de recherche du CHU de Québec and Département d'ophtalmologie, Faculté de médecine, and Regroupement stratégique PROTEO, Université Laval, Québec, Québec, Canada.
authors.forename: Line
authors.initials: L
authors.lastname: Cantin
authors.affiliation: CUO-Recherche, Hôpital du Saint-Sacrement, Centre de recherche du CHU de Québec and Département d'ophtalmologie, Faculté de médecine, and Regroupement stratégique PROTEO, Université Laval, Québec, Québec, Canada. Electronic address: Christian.Salesse@fmed.ulaval.ca.
authors.forename: Christian
authors.initials: C
authors.lastname: Salesse
keywords: Langmuir films; Monolayer; Protein binding; RP2; Retinitis pigmentosa 2