title: Video approach to chemiluminescence detection using a low-cost complementary metal oxide semiconductor (CMOS)-based camera: determination of paracetamol in pharmaceutical formulations.
abstract: A new system for continuous flow chemiluminescence detection, based on the use of a simple and low-priced lens-free digital camera (with complementary metal oxide semiconductor technology) as a detector, is proposed for the quantitative determination of paracetamol in commercial pharmaceutical formulations. Through the camera software, AVI video files of the chemiluminescence emission are captured and then, using friendly ImageJ public domain software (from National Institutes for Health), properly processed in order to extract the analytical information. The calibration graph was found to be linear over the range 0.01-0.10 mg L-1and over the range 1.0-100.0 mg L-1of paracetamol, the limit of detection being 10 μg L-1. No significative interferences were found. Paracetamol was determined in three different pharmaceutical formulations: Termalgin®, Efferalgan® and Gelocatil®. The obtained results compared well with those declared on the formulation label and with those obtained through the official analytical method of British Pharmacopoeia. Graphical abstract Abbreviated scheme of the new chemiluminescence detection system proposed in this paper.
authors.affiliation: Departamento de Farmacia, Universidad CEU-Cardenal Herrera, Avenida Seminario s/n, 46113, Moncada, Valencia, Spain. lahuerta@uchceu.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Lahuerta-Zamora
authors.affiliation: Instituto de Ciencia y Tecnología del Hormigón (ICITECH), Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Mellado-Romero
mesh_terms: D000082:Acetaminophen / Q000032:analysis*; D018712:Analgesics, Non-Narcotic / Q000032:analysis*; D004867:Equipment Design; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D008670:Metals / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors; D013607:Tablets; D014741:Video Recording / Q000191:economics / Q000295:instrumentation* / Q000379:methods
chemical_list: D018712:Analgesics, Non-Narcotic; D008670:Metals; D010087:Oxides; D013607:Tablets; D000082:Acetaminophen
keywords: CMOS camera; Flow chemiluminescence; ImageJ; Pharmaceutical analysis