title: Recognition-mediated particle detection under microfluidic flow with waveguide-coupled 2D photonic crystals: towards integrated photonic virus detectors.
abstract: Label-free biodetection schemes compatible with standard CMOS fabrication methods constitute an important goal, as these are enabling tools for the mass production of high-sensitivity biosensors. Two-dimensional slab photonic crystal (2D slab-PhC) sensors have been posited as ultrahigh-sensitivity detection components, but to date recognition-mediated detection of viruses or simulants under flow has not been demonstrated. We report the design and optimization of a new W1 waveguide-coupled 2D slab-PhC sensor, with a geometry well suited to virus detection. Proof of concept experiments with fluorescent latex particles verified that the sensor could respond to infiltration of a single particle, both in air and under an aqueous cover layer. Subsequent experiments with antibody-functionalized sensors and virus simulants confirmed the ability of the device to detect virus-sized particles under flow via a recognition-mediated process. This work sets the stage for incorporation of 2D slab-PhC sensors into fully integrated photonic sensor systems.
authors.affiliation: Department of Physics, University of Rochester, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Baker
authors.forename: Rashmi
authors.initials: R
authors.lastname: Sriram
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Miller
mesh_terms: D000914:Antibodies, Viral / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D014780:Viruses / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral