title: Biodegradable Poly(Amino Ester) with Aromatic Backbone as Efficient Nonviral Gene Delivery Vectors.
abstract: The development of gene delivery vectors with high efficiency and biocompatibility is one of the critical points of gene therapy. Two biodegradable poly(amino ester)s were synthesized via ring-opening polymerization between low molecular weight (LMW) PEI and diepoxide. The molecular weights of poly(amino ester)s were measured by GPC. Agarose gel retardation assays showed that these materials have good DNA-binding ability and can retard the electrophoretic mobility of plasmid DNA (pDNA) at a weight ratio of 1. The formed polyplexes have proper sizes of around 200 nm and zeta-potential values of about 30-40 mV for cellular uptake. In vitro experiments revealed that polymerP2gave higher transfection efficiency than PEI 25KDa and Lipofectamine 2000 with less toxicity, especially in 293 cells. Results demonstrate that such a type of degradable poly(amino ester) may serve as a promising non-viral gene vector.
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest Minzu University, Chengdu 610041, China. lqchem@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest Minzu University, Chengdu 610041, China. Surongcc@163.com.
authors.forename: Rong-Chuan
authors.initials: RC
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest Minzu University, Chengdu 610041, China. ywjchem@163.com.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Yi
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest Minzu University, Chengdu 610041, China. ywjchem@163.com.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhao
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002021:Buffers; D002412:Cations; D016923:Cell Death / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D000067493:Dynamic Light Scattering; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D055672:Static Electricity; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D002021:Buffers; D002412:Cations; D011108:Polymers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases
keywords: aromatic backbone; biodegradable; cationic polymer; gene delivery; non-viral vectors