title: The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy.
abstract: BACKGROUND
  The Hippo signaling pathway is reported to be involved in angiogenesis, but the roles of the Hippo pathway in diabetic retinopathy have not been addressed. Fufang Xueshuantong Capsule has been used to treat diabetic retinopathy in China; however, the effect of Fufang Xueshuantong Capsule on the Hippo pathway has not been investigated.
  
  
  METHODS
  In this study, diabetes was induced in Sprague-Dawley rats with intraperitoneal injection of streptozotocin. Twenty weeks later, Fufang Xueshuantong Capsule was administered for 12 weeks. When the administration ended, the eyes were isolated for western blot and immunohistochemistry analyses. The levels of P- mammalian sterile 20-like (MST), large tumor suppressor homolog (Lats), P- yes-associated protein (YAP), transcriptional co-activator with PDZ binding motif (TAZ) and TEA domain family members (TEAD) were measured.
  
  
  RESULTS
  Diabetic rats had a decreased P-MST level in the inner plexiform layer and reduced expression of P-YAP in the photoreceptor layers of their eyes. In addition, diabetic rats displayed remarkable increases in Lats, TAZ and TEAD in their retinas. Furthermore, Fufang Xueshuantong Capsule restored the changes in the Hippo pathway.
  
  
  CONCLUSIONS
  The Hippo signaling pathway is important for the progression of diabetic retinopathy and will hopefully be a targeted therapeutic approach for the prevention of diabetic retinopathy.
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China.|Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Gai-Mei
authors.initials: GM
authors.lastname: Hao
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Lv
authors.affiliation: Institute of Chinese Medicine, Beijing University of Chinese medicine, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Wang
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xing
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Chinese Medicine, Beijing University of Chinese medicine, Beijing, China.
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China.
authors.forename: Zheng-Lin
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicine, Beijing, China. wangwei26960@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Chinese Medicine, Beijing University of Chinese medicine, Beijing, China. hanjing8585@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
mesh_terms: D000818:Animals; D002681:China; D003930:Diabetic Retinopathy / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D010330:Patents as Topic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D004365:Drugs, Chinese Herbal; D017346:Protein Serine-Threonine Kinases
keywords: Chinese medicine; Diabetic retinopathy; Hippo