title: Evaluation of GeneXpert MTB/RIF for detecting Mycobacterium tuberculosis in a hospital in China.
abstract: Objective To evaluate the performance of GeneXpert MTB/RIF in diagnosing pulmonary tuberculosis (TB) in China. Methods This cross-sectional study included sputum specimens of 240 suspected TB cases. Specimens were examined by light microscopy for the presence of acid-fast bacilli, which were cultured by the BACTEC MGIT 960 (M960) system and detected by the GeneXpert MTB/RIF assay. The positive rate, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and average turnaround time of methods were evaluated. Results The positive rate was 36.6% (87/238) for the GeneXpert MTB/RIF assay and 34.0% (81/238) by M960 culture, with no significant difference between methods (χ2= 0.33, p > 0.05). According to culture results, sensitivity of the GeneXpert MTB/RIF assay was 84.0% (68/81), specificity was 87.8% (129/147), the PPV was 78.2% (68/87), and the NPV was 87.2% (129/148). The agreement for results between Gene Xpert MTB/RIF and the M960 system was 82.8% and the Kappa value was 0.73. Conclusion The GeneXpert MTB/RIF assay is a simple, rapid, and accurate test for detecting Mycobacterium tuberculosis in sputum specimens.
authors.affiliation: Department of Respiratory Medicine, Zhejiang Hospital, Xihu District, Hangzhou, Zhejiang Province, China.
authors.forename: Tingyu
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Respiratory Medicine, Zhejiang Hospital, Xihu District, Hangzhou, Zhejiang Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Zhejiang Hospital, Xihu District, Hangzhou, Zhejiang Province, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Respiratory Medicine, Zhejiang Hospital, Xihu District, Hangzhou, Zhejiang Province, China.
authors.forename: Qingdong
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000995:Antitubercular Agents / Q000494:pharmacology*; D057205:Automation, Laboratory; D002681:China / Q000453:epidemiology; D003430:Cross-Sectional Studies; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D005260:Female; D006761:Hospitals; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012293:Rifampin / Q000494:pharmacology*; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media; D011933:Reagent Kits, Diagnostic; D012293:Rifampin
keywords: China; GeneXpert MTB/RIF; Tuberculosis