title: A rapid, simple, and accurate plaque assay for human respiratory syncytial virus (HRSV).
abstract: Plaque assays of human respiratory syncytial virus (HRSV) are time-consuming, requiring 4 to 7 days for plaque formation and several hours for dye staining. Here, we describe a simple method by which RSV plaques can be visualized and counted with the naked eye only 2 days after infection of HEp-2 cells. In this assay, the infected cells are stained with monoclonal antibodies and the plaques are developed using diaminobenzidine (DAB). We tested the accuracy of this new plaque assay by comparing the results obtained on days 1, 2, 3, 4, 5, and 6 post-infection. The whole procedure is significantly simpler than the traditional method, with an immunostaining process of around 1.5h. Our method is rapid, accurate, and simple; thus, it has the potential to significantly contribute to studies related to RSV disease.
authors.affiliation: Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Ah-Ra
authors.initials: AR
authors.lastname: Kim
authors.affiliation: Department of Emergency, Yanbian University Hospital, Yanji City, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Piao
authors.affiliation: Department of Pathology, Kyung Hee University Medical Center, Seoul, Republic of Korea.
authors.forename: Ju-Hie
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Republic of Korea. Electronic address: fsquan@khu.ac.kr.
authors.forename: Fu-Shi
authors.initials: FS
authors.lastname: Quan
mesh_terms: D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology / Q000302:isolation & purification*; D013997:Time Factors; D010948:Viral Plaque Assay / Q000379:methods*
keywords: Human respiratory syncytial virus; Immunostaining; Plaque assay