title: Complete genome sequence of Achromobacter insolitus type strain LMG 6003T, a pathogen isolated from leg wound.
abstract: Achromobacter insolitus is a newly described, Gram-negative, small (1-2 mm long) and coccoid pathogen that has been isolated from leg wound. Due to its potential threat to human beings, the type strain of this species LMG 6003T was completely sequenced in this study. The genomic analysis revealed that the genome of LMG 6003T consists of one circular DNA chromosome of 6 492 697 bp with a GC content of 65.10%. The entire genome contains 6208 predicted coding genes, 61 tRNAs and 13 rRNA genes. Comparative genome analysis between LMG 6003T and another selected 10 sequenced Achromobacter revealed that 725 genes only exist in LMG 6003T. In particular, these genes include several important pathogenic-related genes such as Type IV prepilin peptidase (TadV/CpaA), Type VI secretion lipoprotein (VasD) and type 1 fimbriae major subunit (FimA). Genomic island (GI) analysis also suggests the existence of GIs in LMG 6003T. All these results strongly suggest the unique genomic features in LMG 6003T against other Achromobacter species. This report will provide us with an extended understanding of A. insolitus at the genomic level and would be helpful for understanding the evolution of the Achromobacter genus.
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.|Luzhou Branch of National Rice Improvement Center, Luzhou 646100, P.R. China.
authors.forename: Gengmi
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.|Luzhou Branch of National Rice Improvement Center, Luzhou 646100, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.|Luzhou Branch of National Rice Improvement Center, Luzhou 646100, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Yingjiang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Qianhua
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Shumei
authors.initials: S
authors.lastname: You
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Zhaoxiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.|Luzhou Branch of National Rice Improvement Center, Luzhou 646100, P.R. China.
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Department of Translational Medicine, Chengdu Military General Hospital, Chengdu 610083, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wu
authors.affiliation: Luzhou Branch of National Rice Improvement Center, Luzhou 646100, P.R. China.
authors.forename: Jiakui
authors.initials: J
authors.lastname: Zheng
mesh_terms: D042402:Achromobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001482:Base Composition; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D063990:Gene Ontology; D016680:Genome, Bacterial; D044404:Genomic Islands; D006801:Humans; D007866:Leg / Q000382:microbiology; D058977:Molecular Sequence Annotation; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D000069376:Type VI Secretion Systems / Q000235:genetics / Q000378:metabolism; D000073336:Whole Genome Sequencing
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular; D012336:RNA, Ribosomal, 16S; D000069376:Type VI Secretion Systems; C063055:fimbrillin; D036781:Fimbriae Proteins; D012343:RNA, Transfer; D010450:Endopeptidases; C074273:type IV prepilin peptidase