title: Isolation of High-Molecular-Weight DNA Using Organic Solvents.
abstract: Perhaps the most basic of all procedures in molecular cloning is the purification of nucleic acids. The key step, the removal of proteins, can often be performed simply by extracting aqueous solutions of nucleic acids with phenol:chloroform and chloroform.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
authors.forename: Joseph
authors.initials: J
authors.lastname: Sambrook
mesh_terms: D002725:Chloroform; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D008970:Molecular Weight; D019800:Phenol; D012997:Solvents
chemical_list: D012997:Solvents; D019800:Phenol; D002725:Chloroform; D004247:DNA