title: Synthesis of glycinamides using protease immobilized magnetic nanoparticles.
abstract: In the present investigation,Bacillus subtiliswas isolated from slaughterhouse waste and screened for the production of protease enzyme. The purified protease was successfully immobilized on magnetic nanoparticles (MNPs) and used for the synthesis of series of glycinamides. The binding and thermal stability of protease on MNPs was confirmed by FTIR spectroscopy and TGA analysis. The surface morphology of MNPs before and after protease immobilization was carried out using SEM analysis. XRD pattern revealed no phase change in MNPs after enzyme immobilization. The processing parameters for glycinamides synthesis viz. temperature, pH, and time were optimized using Response Surface Methodology (RSM) by using Design Expert (9.0.6.2). The maximum yield of various amides 2 butyramidoacetic acid (AMD-1,83.4%), 2-benzamidoacetic acid (AMD-2,80.5%) and 2,2'((carboxymethyl) amino)-2-oxoethyl)-2-hydroxysuccinyl)bis(azanediyl))diacetic acid (AMD-3,80.8%) formed was observed at pH-8, 50 °C and 30 min. The synthesized immobilized protease retained 70% of the initial activity even after 8 cycles of reuse.
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
authors.forename: Abha
authors.initials: A
authors.lastname: Sahu
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
authors.forename: Pallavi Sharad
authors.initials: PS
authors.lastname: Badhe
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
authors.forename: Ravindra
authors.initials: R
authors.lastname: Adivarekar
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
authors.forename: Mayur Ramrao
authors.initials: MR
authors.lastname: Ladole
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India.
authors.forename: Aniruddha Bhalchandra
authors.initials: AB
authors.lastname: Pandit
keywords: Glycinamides; Immobilization; Magnetic nanoparticles; Protease; Response surface methodology