title: Multicolor bioimaging with biosynthetic zinc nanoparticles and their application in tumor detection.
abstract: Because they generate excellent images, nanoparticles (NPs), especially biosynthesized NPs, provide a new solution for tumor imaging. In this research, we unveil a novel type of biosynthesized NPs featuring multicolor fluorescence. These NPs exhibit little cytotoxicity to cells. The explored NPs, designated Zn-ZFP-GST NPs (Zinc NPs in abbreviation), are generated from leukemia cells treated with a Zn2+solution, while zinc-finger protein and glutathione S-transferase (GST) were also identified in the Zinc NPs. Under near-UV illumination, the Zinc NPs simultaneously emit green, yellow, and red fluorescence. In addition, the intensity of the fluorescence increases with the existence of sulfides. Besides, the NPs are encapsulated by microvesicles (MVs) shed from the plasma membrane. As observed in whole-body research of nude mice, the NP-MVs migrate via blood circulation and are distinguished by their fluorescent signals. Furthermore, the folic acid (FA) &AVR2 (human VEGF antibody)-coated NP-MVs are exploited to target the tumor location, and the feasibility of this approach has been confirmed empirically. The Zinc NPs shed light on an alternative solution to tumor detection.
authors.affiliation: Wuxi School of Medicine, Jiangnan University, 214122, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Yi-Zhou
authors.initials: YZ
authors.lastname: Wu
authors.affiliation: Department of Biochemistry, Qiannan Medical College for Nationalities, Duyun 558000, China.
authors.forename: Xianyun
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Xu
authors.affiliation: The Center for Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Geng
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Tongxing
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Xiaobang
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Nanjing Maternity and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, 210004, Nanjing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D005453:Fluorescence; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D007938:Leukemia / Q000000981:diagnostic imaging*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D013440:Sulfides; D005492:Folic Acid; D015032:Zinc