title: Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.
abstract: Legionella pneumophila is the main causative agent of Legionnaires' disease, which is a severe multi-system disease with pneumonia as the primary manifestation. We designed a recombinant Mip-PilE-FlaA dominant epitopes vaccine against Legionella pneumophila to prevent the disease and evaluated its immunogenicity and protective immunity. The protein structures of Mip, PilE and FlaA were analyzed using a computer, and the gene sequences of the dominant epitopes of the three proteins were selected to construct and optimize the vaccine. The optimized mip, pilE, flaA and recombinant mip-pilE-flaA gene sequences were cloned, expressed and purified. The purified proteins were used as dominant epitopes vaccines to immunize BALB/c mice and determine the protective immunity and immunogenicity of these purified proteins. The identification confirmed that the recombinant mip-pilE-flaA was successfully cloned and expressed. ELISA revealed that the Mip-PilE-FlaA group produced the highest IgG response, and this protein may considerably improve the production of some cytokines in BALB/c mice. Histopathology analyses of lungs from mice immunized with Mip-PilE-FlaA revealed a certain protective effect. Our work demonstrated that the recombinant dominant epitopes of Mip-PilE-FlaA exhibited strong immunogenicity and immune protection, and this protein may be an efficient epitopes vaccine candidate against Legionella pneumophila.
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Jinlei
authors.initials: J
authors.lastname: He
authors.affiliation: First Department of Hepatobiliary Surgery, Qinghai University Affiliated Hospital, Medical College of Qinghai University, Xining, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, China. Electronic address: jpchen007@163.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D003198:Computer Simulation; D005260:Female; D005408:Flagellin / Q000235:genetics; D006801:Humans; D056724:Immunity, Humoral; D007114:Immunization; D016056:Immunodominant Epitopes / Q000235:genetics / Q000378:metabolism; D016952:Legionella pneumophila / Q000472:pathogenicity / Q000502:physiology*; D007877:Legionnaires' Disease / Q000276:immunology* / Q000517:prevention & control; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019696:Peptidylprolyl Isomerase / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016056:Immunodominant Epitopes; C000591933:PILE protein, Legionella pneumophila; D011994:Recombinant Proteins; D037521:Virulence Factors; D005408:Flagellin; C067138:flaA protein, bacteria; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase
keywords: Epitopes vaccine; FlaA; Immunity; Legionella pneumophila; Mip; PilE