title: Genome-wide identification of membrane-bound fatty acid desaturase genes in Gossypium hirsutum and their expressions during abiotic stress.
abstract: Membrane-bound fatty acid desaturases (FADs) are of great importance and play multiple roles in plant growth and development. In the present study, 39 full-length FAD genes, based on database searches, were identified in tetraploid upland cotton (Gossypium hirsutum L.) and were phylogenetically clustered into four subfamilies. Genomic localization revealed that 34 genes were mapped on 22 chromosomes, and five genes were positioned on the scaffold sequences. The FAD genes of G. hirsutum in the same subfamily had similar gene structures. The structures of paralogous genes were considerably conserved in exons number and introns length. It was suggested that the FAD gene families in G. hirsutum might be duplicated mainly by segmental duplication. Moreover, the FAD genes were differentially expressed in different G. hirsutum tissues in response to different levels of salt and cold stresses, as determined by qRT-PCR analysis. The identification and functional analysis of FAD genes in G. hirsutum may provide more candidate genes for genetic modification.
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, Pakistan.
authors.forename: M K
authors.initials: MK
authors.lastname: Daud
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Agronomy, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shuijin
authors.initials: S
authors.lastname: Zhu
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D005091:Exons / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics*; D020440:Gene Duplication / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D003368:Gossypium / Q000235:genetics*; D007438:Introns / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D044943:Fatty Acid Desaturases