title: One-step synthesis of high-yield biodiesel from waste cooking oils by a novel and highly methanol-tolerant immobilized lipase.
abstract: This study reported a novel immobilized MAS1 lipase from marine Streptomyces sp. strain W007 for synthesizing high-yield biodiesel from waste cooking oils (WCO) with one-step addition of methanol in a solvent-free system. Immobilized MAS1 lipase was selected for the transesterification reactions with one-step addition of methanol due to its much more higher biodiesel yield (89.50%) when compared with the other three commercial immobilized lipases (<10%). The highest biodiesel yield (95.45%) was acquired with one-step addition of methanol under the optimized conditions. Moreover, it was observed that immobilized MAS1 lipase retained approximately 70% of its initial activity after being used for four batch cycles. Finally, the obtained biodiesel was further characterized using FT-IR,1H and13C NMR spectroscopy. These findings indicated that immobilized MAS1 lipase is a promising catalyst for biodiesel production from WCO with one-step addition of methanol under high methanol concentration.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qin
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Daoming
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: yonghw@scut.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D056804:Biofuels; D003296:Cooking; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D008049:Lipase / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D010938:Plant Oils; D008049:Lipase; D000432:Methanol
keywords: Biodiesel; Characterization; Immobilized MAS1 lipase; Transesterification; Waste cooking oils