title: Chitosan mediated enhancement of hydrolysable tannin in Phyllanthus debilis Klein ex Willd via plant cell suspension culture.
abstract: Phyllanthus debilis Klein ex Willd. is wild medicinal plant used in the traditional system of medicine. This plant has been actively used for hepatoprotection and to cure many diseases including jaundice and so on; which leads to complete extinction of this particular species. Therefore, the chitosan mediated cost effective cell suspension method has been developed for the production of hydrolysable tannin. The hydrolysable tannins are the main therapeutically active constituents with antioxidant, anticancer, and antimicrobial properties. An in vitro cell suspension culture was optimized by adding chitosan for production of hydrolysable tannin. According to the growth kinetics, a maximum biomass of 4.46±0.06g fresh cell weight and 1.33±0.04g dry cell weight were obtained from the optimal suspension medium consisted of MS medium+0.5mgL-1BAP+1.5mgL-1NAA. Chitosan was treated at the stationary phase which leads to the highest accumulation of hydrolysable tannin compared to the untreated control. Hydrolysable tannin was observed and compared using HPLC at the Rt of 4.91 in both chitosan treated and untreated cells. This is the first ever report where use of chitosan has been done to enhance the production of the hydrolysable tannin in P. debilis using cell suspension culture technique.
authors.affiliation: Department of Botany, Jamal Mohamed College, Tiruchirappallai, Tamil Nadu 620020, India. Electronic address: malai86man@gmail.com.
authors.forename: Malayaman
authors.initials: M
authors.lastname: V
authors.affiliation: Department of Botany, Jamal Mohamed College, Tiruchirappallai, Tamil Nadu 620020, India. Electronic address: nsisubalan@gmail.com.
authors.forename: Sisubalan
authors.initials: S
authors.lastname: N
authors.affiliation: Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu 641 029, India. Electronic address: sensenthilsen@gmail.com.
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: R P
authors.affiliation: Department of Botany, Jamal Mohamed College, Tiruchirappallai, Tamil Nadu 620020, India. Electronic address: sheikmsss@gmail.com.
authors.forename: Sheik Mohamed
authors.initials: SM
authors.lastname: S
authors.affiliation: Kirnd Institute of Research and Development Pvt. Ltd, Tiruchirappalli, Tamil Nadu 620020, India. Electronic address: biotechranjith@gmail.com.
authors.forename: Ranjithkumar
authors.initials: R
authors.lastname: R
authors.affiliation: Department of Botany, Jamal Mohamed College, Tiruchirappallai, Tamil Nadu 620020, India. Electronic address: drghobashjmc@gmail.com.
authors.forename: Ghouse Basha
authors.initials: GB
authors.lastname: M
mesh_terms: D018929:Cell Culture Techniques; D048271:Chitosan / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D029803:Phyllanthus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013535:Suspensions; D013634:Tannins / Q000096:biosynthesis*
chemical_list: D013535:Suspensions; D013634:Tannins; D048271:Chitosan
keywords: Cell suspension culture; Chitosan; Hydrolysable tannin; Phyllanthus debilis