title: Effects of IFN-γ on cell growth and the expression of ADAM33 gene in human embryonic lung Mrc-5 fibroblasts in vitro.
abstract: To investigate the effects of interferon-γ (IFN-γ) on the proliferation and viability of human embryonic lung Mrc-5 fibroblasts in vitro and the expression of the A Disintegrin and Metalloprotease 33 (ADAM33) gene and to explore the mechanism of airway remodeling.
  
  
  
  Mrc-5 fibroblasts were sensitized with Dermatophagoides farinae 1 (Derf1) in vitro to mimic in vivo conditions observed in bronchial asthma. An inverted fluorescence microscope was used to observe changes in cell morphology before and after treatment. The viability of Mrc-5 cells was tested using the Cell Counting kit-8 (CCK8). Expression of the ADAM33 gene and protein in Mrc-5 cells was assessed using qPCR and Western blotting, respectively.
  
  
  
  Different concentrations of Derf1 increased cell growth and the expression of the ADAM33 gene in Mrc-5 cells, and these changes were most obvious in the 10 µg/ml group. In contrast, IFN-γ decreased cell growth and the expression of the ADAM33 gene in both Mrc-5 cells and Derf1-induced Mrc-5 cells, and these changes were most obvious in the 10 ng/ml group. The negative effects of 10 ng/ml IFN-γ were the most significant at 32 hours.
  
  
  
  Derf1-induced Mrc-5 cells successfully imitated the in vivo conditions observed in patients with asthma. IFN-γ inhibited the proliferation and viability of Mrc-5 cells, and Derf1-induced Mrc-5 cells were more sensitive to IFN-γ treatment. IFN-γ treatment significantly downregulated the expression of the ADAM33 gene in a concentration- and time-dependent manner. IFN-γ may participate in airway remodeling in asthma by regulating the expression of the ADAM33 gene.
authors.affiliation: a Department of Pediatrics, Sun Yat-sen Memorial Hospital , Sun Yat-sen University , Guangzhou , Guangdong , China.|b Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangzhou Higher Education Institutes of Sun Yat-sen University , Guangzhou , Guangdong , China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.affiliation: a Department of Pediatrics, Sun Yat-sen Memorial Hospital , Sun Yat-sen University , Guangzhou , Guangdong , China.|b Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangzhou Higher Education Institutes of Sun Yat-sen University , Guangzhou , Guangdong , China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Liang
authors.affiliation: a Department of Pediatrics, Sun Yat-sen Memorial Hospital , Sun Yat-sen University , Guangzhou , Guangdong , China.|b Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangzhou Higher Education Institutes of Sun Yat-sen University , Guangzhou , Guangdong , China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Huang
authors.affiliation: a Department of Pediatrics, Sun Yat-sen Memorial Hospital , Sun Yat-sen University , Guangzhou , Guangdong , China.|b Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangzhou Higher Education Institutes of Sun Yat-sen University , Guangzhou , Guangdong , China.
authors.forename: Baojing
authors.initials: B
authors.lastname: Wu
authors.affiliation: b Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangzhou Higher Education Institutes of Sun Yat-sen University , Guangzhou , Guangdong , China.|c Department of Gastroenterology, Sun Yat-sen Memorial Hospital , Sun Yat-sen University , Guangzhou , Guangdong , China.
authors.forename: Yingqiang
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D051722:ADAM Proteins / Q000276:immunology / Q000378:metabolism*; D056151:Airway Remodeling / Q000187:drug effects / Q000276:immunology*; D000485:Allergens / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D005347:Fibroblasts; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D008168:Lung / Q000276:immunology / Q000473:pathology; D032389:Myocytes, Smooth Muscle
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D007371:Interferon-gamma; D051722:ADAM Proteins; C450441:ADAM33 protein, human
keywords: ADAM33 gene; Derf1; IFN-γ; Mrc-5 cells; cell proliferation