title: A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham.
abstract: Oxyresveratrol is a major active compound in the heartwood of Artocarpus lacucha. It plays an important role in anti-tyrosinase, antioxidant, anti-inflammatory, antiviral and neuroprotective properties. There are many A. lacucha commercial products available on the market for skin whitening and anti-aging effects. To evaluate the quality of raw material from the plant, a monoclonal antibody (MAb) against oxyresveratrol was generated in this study. The immunogen was prepared by the Mannich reaction for the conjugation of oxyresveratrol and cationized bovine serum albumin (cBSA). The conjugation of oxyresveratrol-cBSA at a ratio of 1:50 was used for the immunization. The novel MAb (E4) was specific to oxyresveratrol and resveratrol. An indirect competitive enzyme-linked immunosorbent assay (ELISA) using the MAb (E4) was developed for the determination of oxyresveratrol. The linear range for the measurement of oxyresveratrol was 63-500 ng/mL and the precision (% relative standard deviation) was found to be <10% with the percentages of recovery from 95.93-103.55%. According to the validation analysis, the established ELISA can be applied for the determination of oxyresvertrol in the heartwood of A. lacucha and samples of the traditional drug Puag-Haad. With reliability and high sensitivity, this assay can provide an alternative approach for the quantitative analysis of oxyresveratrol in A. lacucha samples.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Tharita
authors.initials: T
authors.lastname: Kitisripanya
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Chadathorn
authors.initials: C
authors.lastname: Inyai
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Supaluk
authors.initials: S
authors.lastname: Krittanai
authors.affiliation: Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Kittisak
authors.initials: K
authors.lastname: Likhitwitayawuid
authors.affiliation: Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Boonchoo
authors.initials: B
authors.lastname: Sritularak
authors.affiliation: Laboratory of Medicinal Chemistry, Program in Chemical Biology, Chulabhorn Research Institute, Chulabhorn Graduate Institute, 54 Kampaeng Phet 6 Road, Bangkok, Thailand.
authors.forename: Poonsakdi
authors.initials: P
authors.lastname: Ploypradith
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morimoto
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. waraporn@kku.ac.th.|Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand. waraporn@kku.ac.th.
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000975:Antioxidants; D000998:Antiviral Agents; D031622:Artocarpus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007114:Immunization; D007118:Immunoassay / Q000379:methods; D008297:Male; D008807:Mice, Inbred BALB C; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D000975:Antioxidants; D000998:Antiviral Agents; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D014442:Monophenol Monooxygenase; D000077185:Resveratrol
keywords: Artocarpus lacucha; Enzyme-linked immunosorbent assay (ELISA); Monoclonal antibody; Oxyresveratrol