title: Characterization of maize genotypes for genetic diversity on the basis of inter simple sequence repeats.
abstract: Genetic diversity in crops is essential to make improvements related to superior germplasms. Implementation of molecular markers to identify suitable genotypes speeds up the breeding progress by enhancing selection efficiency. This study was carried out to probe genetic diversity among 21 maize genotypes using 20 inter simple sequence repeat (ISSR) markers. We identified a total of 190 polymorphic bands with an average of 9.5 alleles per primer. The highest number of polymorphic bands (17) was found using ISSR marker UBC-10, whereas the lowest number of polymorphic bands (4) was found using UBC-809. The coefficient of genetic similarity ranged from 0.888 to 0.118%. The highest similarity was found between accessions 12 (015224) and 9 (015114), whereas the lowest similarity was found between genotypes 20 (EV-5098) and 14 (015030). The polymorphism information content ranged from 0.17 to 0.47. A dendrogram was generated based on Jaccard's distance matrix. The genotypes were found to group into two major clusters that could be further partitioned into two sub-clusters. Genotypes located within the same cluster are genetically more closely related to each other. The present study efficiently identified diverse genotypes that may be used for creating new varieties with distinct characteristics. The identified genotypes could be used as parents for future development of diverse populations.
authors.affiliation: PARC Research and Training Station, Pakistan Agriculture Research Council, Multan, Pakistan.
authors.forename: R W
authors.initials: RW
authors.lastname: Muhammad
authors.affiliation: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Qayyum
authors.affiliation: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Ahmad
authors.affiliation: PARC Research and Training Station, Pakistan Agriculture Research Council, Multan, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Hamza
authors.affiliation: Arid Zone Research Institute, PARC, Bahawalpur.
authors.forename: M
authors.initials: M
authors.lastname: Yousaf
authors.affiliation: Arid Zone Research Institute, PARC, Bahawalpur.
authors.forename: B
authors.initials: B
authors.lastname: Ahmad
authors.affiliation: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Younas
authors.affiliation: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: W
authors.initials: W
authors.lastname: Malik
authors.affiliation: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Liaqat
authors.affiliation: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: E
authors.initials: E
authors.lastname: Noor
mesh_terms: D000483:Alleles; D018556:Crops, Agricultural / Q000235:genetics; D005787:Gene Frequency; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats; D010802:Phylogeny; D000069600:Plant Breeding; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D003313:Zea mays / Q000235:genetics*
chemical_list: D005819:Genetic Markers