title: A Sphingosine-type cerebroside in Clavicorona pyxidata induce fruit body formation.
abstract: Clavicorona pyxidata is a wild edible and medicinal mushroom that is rich in bioactive natural products and has thus been extensively used as traditional medicine in China. The present study has determined that the organic crude extract prepared from a fermented culture of C. pyxidata imparted auto-inhibitory effects on mycelial growth and then induced the formation of fruiting bodies. By monitoring bioactivity, one compound was isolated via successive chromatography over silica gel, Sephadex LH-20, and Cl8-reversed phase silica gel and was identified as a known sphingosine-type cerebroside by nuclear magnetic resonance (NMR) and physicochemical data, namely, (4E, 8E)-N-D-2'-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine. The application of this cerebroside at a concentration of 200 μg/disc paper resulted in the inhibition of aerial hyphal growth of C. pyxidata. The findings of the present study indicated that this C. pyxidata cerebroside is a fruiting body-inducing substance (FIS).
authors.affiliation: a Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences , Fujian Normal University , Fuzhou , P.R. China.
authors.forename: Yongbiao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: b Xiamen Medical College , Xiamen , P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: a Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences , Fujian Normal University , Fuzhou , P.R. China.
authors.forename: Liufeng
authors.initials: L
authors.lastname: Wei
authors.affiliation: a Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences , Fujian Normal University , Fuzhou , P.R. China.
authors.forename: Mianmian
authors.initials: M
authors.lastname: Shi
authors.affiliation: a Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences , Fujian Normal University , Fuzhou , P.R. China.
authors.forename: Jiyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: a Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences , Fujian Normal University , Fuzhou , P.R. China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Huang
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000187:drug effects*; D002554:Cerebrosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002681:China; D005285:Fermentation; D005638:Fruit / Q000737:chemistry; D048690:Fruiting Bodies, Fungal / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D013110:Sphingosine / Q000737:chemistry*
chemical_list: D002554:Cerebrosides; D013110:Sphingosine
keywords: Clavicorona pyxidata; cerebroside; fruiting body formation