title: Comparison of Short-Term Effects of Diquafosol and Rebamipide on Mucin 5AC Level on the Rabbit Ocular Surface.
abstract: To investigate the short-term effects of 2 new secretagogue eye drops for dry eye, 3% diquafosol tetrasodium ophthalmic solution (diquafosol) and 2% rebamipide ophthalmic suspension (rebamipide), on the concentration of mucin 5AC (MUC5AC) in rabbit tear fluid and conjunctival goblet cells.
  
  
  
  One dose of artificial tears, diquafosol or rebamipide, was instilled into 8 eyes of Japanese white rabbits. MUC5AC concentration in the tear fluid was examined using the enzyme-linked immunosorbent assay 15 min after instillation and compared with 8 untreated controls. Impression cytology was performed to measure the number of periodic acid Schiff (PAS)-positive cells and the ratio of the PAS-positive area using image analysis software. Statistical comparison was performed using ANOVA with post hoc analysis with the Tukey's test.
  
  
  
  After 15 min, only diquafosol significantly (P ≤ 0.01) increased the MUC5AC level in the tear fluid. Although no drug affected the number of PAS-positive cells, the ratio of the PAS-positive area decreased significantly (P ≤ 0.01) only in the diquafosol group.
  
  
  
  These data indicated that more PAS-positive MUC5AC was released into the tear fluid from the goblet cells by diquafosol than by rebamipide. There is a difference in the induction pattern of MUC5AC into the tears from the goblet cells between these eye drops.
authors.affiliation: 1 Department of Ophthalmology, School of Medicine, Toho University , Tokyo, Japan .|2 Department of Ophthalmology, Toho University Sakura Medical Center , Sakura, Chiba, Japan .
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hori
authors.affiliation: 3 Research and Development Division, Santen Pharmaceutical Co., Ltd. , Ikoma, Nara, Japan .
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Kageyama
authors.affiliation: 3 Research and Development Division, Santen Pharmaceutical Co., Ltd. , Ikoma, Nara, Japan .
authors.forename: Asuka
authors.initials: A
authors.lastname: Sakamoto
authors.affiliation: 1 Department of Ophthalmology, School of Medicine, Toho University , Tokyo, Japan .
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Shiba
authors.affiliation: 3 Research and Development Division, Santen Pharmaceutical Co., Ltd. , Ikoma, Nara, Japan .
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: 2 Department of Ophthalmology, Toho University Sakura Medical Center , Sakura, Chiba, Japan .
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Maeno
mesh_terms: D000409:Alanine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003228:Conjunctiva / Q000187:drug effects* / Q000378:metabolism; D020397:Goblet Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D055271:Mucin 5AC / Q000032:analysis* / Q000378:metabolism; D009883:Ophthalmic Solutions / Q000008:administration & dosage / Q000494:pharmacology*; D011122:Polyphosphates / Q000008:administration & dosage / Q000494:pharmacology*; D015363:Quinolones / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D013499:Surface Properties; D013666:Tears / Q000187:drug effects*; D014500:Uracil Nucleotides / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D055271:Mucin 5AC; D009883:Ophthalmic Solutions; D011122:Polyphosphates; D015363:Quinolones; D014500:Uracil Nucleotides; C403315:diquafosol; C052785:rebamipide; D000409:Alanine
keywords: diquafosol tetrasodium; goblet cell; mucin 5AC; rabbit; rebamipide; short-term effect