title: Genetic polymorphisms in TLR1, TLR2, TLR4, and TLR10 of Helicobacter pylori-associated gastritis: a prospective cross-sectional study in Thailand.
abstract: The toll-like receptors (TLRs) mediate the recognition of Helicobacter pylori and initiate the innate immune response to infection. We hypothesized those genetic polymorphisms in the TLR1, TLR2, TLR4, and TLR10 influence bacterial infection, affecting susceptibility H. pylori to disease outcomes. Genomic DNA was extracted and genotypes of TLR1 (rs4833095), TLR2 (rs3804099 and rs3804100), TLR4 (rs10759932), and TLR10 (rs10004195) polymorphism were detected by the TagMan single-nucleotide epolymorphisms genotyping assay using the real-time PCR hybridization probe method. The TLR1 (rs4833095), C allele and the TLR10 (rs10004195), A allele frequency was significantly increased risk in the H. pylori infection group (odds ratio=1.76, 95% confidence interval=1.84-2.15, P=0.01 and odds ratio=1.81, 95% confidence interval=1.18-3.26, P=0.04, respectively). The TLR1 (rs4833095), C allele and TLR10 (rs10004195), A allele are susceptible TLRs polymorphisms in the Thai population. These findings suggest that TLR1 rs4833095 and TLR10 rs10004195 may play crucial roles in H. pylori susceptibility and gastric pathogenesis.
authors.affiliation: Department of Surgery.|Suranaree University of Technology Hospital.
authors.forename: Taweesak
authors.initials: T
authors.lastname: Tongtawee
authors.affiliation: Department of Surgery.
authors.forename: Theeraya
authors.initials: T
authors.lastname: Bartpho
authors.affiliation: Family Medicine and Community Medicine.|Suranaree University of Technology Hospital.
authors.forename: Soraya
authors.initials: S
authors.lastname: Kaewpitoon
authors.affiliation: Faculty of Public Health, Vongchavalitkul University, Nakhon Ratchasima, Thailand.
authors.forename: Natthawut
authors.initials: N
authors.lastname: Kaewpitoon
authors.affiliation: School of Pathology.|Suranaree University of Technology Hospital.
authors.forename: Chavaboon
authors.initials: C
authors.lastname: Dechsukhum
authors.affiliation: School of Preclinic.
authors.forename: Wilairat
authors.initials: W
authors.lastname: Leeanansaksiri
authors.affiliation: Family Medicine and Community Medicine.|Suranaree University of Technology Hospital.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Loyd
authors.affiliation: Medical Technology and Medical Biochemistry, Institute of Medicine.
authors.forename: Krajang
authors.initials: K
authors.lastname: Talabnin
authors.affiliation: Department of Surgery.|Suranaree University of Technology Hospital.
authors.forename: Likit
authors.initials: L
authors.lastname: Matrakool
authors.affiliation: Department of Surgery.|Suranaree University of Technology Hospital.
authors.forename: Sukij
authors.initials: S
authors.lastname: Panpimanmas
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011230:Precancerous Conditions / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D015995:Prevalence; D011446:Prospective Studies; D013274:Stomach Neoplasms / Q000473:pathology / Q000517:prevention & control; D013785:Thailand / Q000453:epidemiology; D051194:Toll-Like Receptor 1 / Q000235:genetics*; D051201:Toll-Like Receptor 10 / Q000235:genetics*; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C000629912:TLR1 protein, human; C475955:TLR10 protein, human; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051194:Toll-Like Receptor 1; D051201:Toll-Like Receptor 10; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4