title: Structural modelling and phylogenetic analyses ofPgeIF4A2(Eukaryotic translation initiation factor) fromPennisetum glaucumreveal signature motifs with a role in stress tolerance and development.
abstract: Eukaryotic translation initiation factor 4A (eIF4A) is an indispensable component of the translation machinery and also play a role in developmental processes and stress alleviation in plants and animals. Different eIF4A isoforms are present in the cytosol of the cell, namely, eIF4A1, eIF4A2, and eIF4A3 and their expression is tightly regulated in cap-dependent translation. We revealed the structural model of PgeIF4A2 protein using the crystal structure of Homo sapiens eIF4A3 (PDB ID: 2J0S) as template by Modeller 9.12. The resultant PgeIF4A2 model structure was refined by PROCHECK, ProSA, Verify3D and RMSD that showed the model structure is reliable with 77 % amino acid sequence identity with template. Investigation revealed two conserved signatures for ATP-dependent RNA Helicase DEAD-box conserved site (VLDEADEML) and RNA helicase DEAD-box type, Q-motif in sheet-turn-helix and α-helical region respectively. All these conserved motifs are responsible for response during developmental stages and stress tolerance in plants.
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India;; Plant Molecular Biology Lab, Department of Botany, University of Delhi, New Delhi, India.
authors.forename: Aakrati
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: Plant Molecular Biology Lab, Department of Botany, University of Delhi, New Delhi, India.
authors.forename: Yashwanti
authors.initials: Y
authors.lastname: Mudgil
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Pandey
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Dhirendra
authors.initials: D
authors.lastname: Fartyal
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Malireddy K
authors.initials: MK
authors.lastname: Reddy
keywords: Eukaryotic initiation factor; Pennisetum glaucum; eIF4A2; helicase; structural modelling