title: The use of Ościk's equation to describe the retention in reversed phase liquid chromatography.
abstract: The Ościk's equation based on the theory of conformal solutions was used to describe the chromatographic process in RP-HPLC. Theoretically calculated values of the logarithms of retention factors (logk) were compared with experimental values for a group of aromatic substances, i.e. alkylbenzenes, PAHs, and polar benzene derivatives. Studies were conducted using two columns: RP-18 and RP-4, as well as water-methanol and water-acetonitrile mixtures as mobile phases. The applicability of Everett's equation to describe the composition of surface phase was also verified. For this purpose, the excess methanol adsorption isotherm on LiChrospher RP-18e, was experimentally determined.
authors.affiliation: Institute of Biotechnology, John Paul II Catholic University of Lublin, 1i Konstantynów Street, Lublin, 20-708, Poland. Electronic address: mirekkwi@kul.lublin.pl.
authors.forename: Ludomir
authors.initials: L
authors.lastname: Kwietniewski
authors.affiliation: Institute of Biotechnology, John Paul II Catholic University of Lublin, 1i Konstantynów Street, Lublin, 20-708, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rycyk
mesh_terms: D000097:Acetonitriles / Q000032:analysis / Q000737:chemistry*; D000327:Adsorption; D001555:Benzene Derivatives / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D000432:Methanol / Q000032:analysis / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D001555:Benzene Derivatives; D011084:Polycyclic Aromatic Hydrocarbons; D014867:Water; D000432:Methanol; C032159:acetonitrile
keywords: Adsorption of acetonitrile; Adsorption of methanol; Aromatic solutes; Excess isotherm; Theory of RP LC