title: Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe3O4-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD.
abstract: Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.
authors.affiliation: Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran; Department of Microbiology, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Heidarizadeh
authors.affiliation: Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran; Department of Chemistry, Faculty of Science, Urmia University, Urmia 57159-165, Iran. Electronic address: esmaildostkhah@gmail.com.
authors.forename: Esmail
authors.initials: E
authors.lastname: Doustkhah
authors.affiliation: Organic and Nano Group (ONG), Department of Chemistry, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran. Electronic address: rostamnia@maragheh.ac.ir.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Rostamnia
authors.affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.
authors.forename: Parisa Fathi
authors.initials: PF
authors.lastname: Rezaei
authors.affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, PO Box 55181-83111, Maragheh, Iran.
authors.forename: Farshad Darvishi
authors.initials: FD
authors.lastname: Harzevili
authors.affiliation: Department of Chemistry, Faculty of Science, Urmia University, Urmia 57159-165, Iran. Electronic address: bzeynizadeh@gmail.com.
authors.forename: Behzad
authors.initials: B
authors.lastname: Zeynizadeh
mesh_terms: D000818:Animals; D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006868:Hydrolysis; D007848:Laurates / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D053761:Nanocomposites / Q000737:chemistry; D009578:Nitrobenzenes / Q000378:metabolism*; D010087:Oxides / Q000737:chemistry; D011487:Protein Conformation; D013552:Swine; D013859:Thiocarbamates / Q000737:chemistry*
chemical_list: C484326:4-nitrophenyl dodecanoate; D004800:Enzymes, Immobilized; D007848:Laurates; D058185:Magnetite Nanoparticles; D009578:Nitrobenzenes; D010087:Oxides; D013859:Thiocarbamates; D006108:Graphite; D008049:Lipase
keywords: Dithiocarbamate; Enzyme immobilization; Graphene oxide; Lipase; Nanocomposite