title: Culture and Expansion of Primary Undifferentiated Spermatogonial Stem Cells.
abstract: This protocol describes a culture method for supporting the maintenance and expansion of a primary mouse undifferentiated spermatogonial population containing spermatogonial stem cells (SSCs). The doubling time for SSCs in culture is relatively slow. Once established, SSC lines are split 1:2 to 1:4 every 7 d. Therefore, the time required to generate sufficient SSCs for experimentation can be considerable and requires careful planning.
authors.forename: Shaun
authors.initials: S
authors.lastname: Goodyear
authors.forename: Ralph
authors.initials: R
authors.lastname: Brinster
mesh_terms: D000072956:Adult Germline Stem Cells / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D051379:Mice