title: Imaging Translational and Post-Translational Gene Regulatory Dynamics in Living Cells with Antibody-Based Probes.
abstract: Antibody derivatives, such as antibody fragments (Fabs) and single-chain variable fragments (scFvs), are now being used to image traditionally hard-to-see protein subpopulations, including nascent polypeptides being translated and post-translationally modified proteins. This has allowed researchers to directly image and quantify, for the first time, translation initiation and elongation kinetics with single-transcript resolution and the temporal ordering and kinetics of post-translational histone and RNA polymerase II modifications. Here, we review these developments and discuss the strengths and weaknesses of live-cell imaging with antibody-based probes. Further development of these probes will increase their versatility and open new avenues of research for dissecting complex gene regulatory dynamics.
authors.affiliation: Department of Biochemistry and Molecular Biology and the Institute for Genome Architecture and Function, Colorado State University, Fort Collins, CO, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Lyon
authors.affiliation: Department of Biochemistry and Molecular Biology and the Institute for Genome Architecture and Function, Colorado State University, Fort Collins, CO, USA. Electronic address: Tim.Stasevich@colostate.edu.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Stasevich
mesh_terms: D000107:Acetylation; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics* / Q000276:immunology; D007700:Kinetics; D010455:Peptides / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000235:genetics; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology
chemical_list: D007128:Immunoglobulin Fragments; D010455:Peptides; D057127:Single-Chain Antibodies