title: Effects of atmospheric-pressure non-thermal bio-compatible plasma and plasma activated nitric oxide water on cervical cancer cells.
abstract: Atmospheric-pressure non-thermal bio-compatible plasma is a partially ionized gas with electrically charged particles. Previous studies demonstrated that dielectric barrier discharge (DBD) plasma could induce apoptosis of various cancer cells, in particular demonstrating the selective cytotoxicity of cancer cells over normal cells. Therefore, DBD plasma can be considered as a potential cancer treatment method for clinical applications. We previously developed a microwave jet plasma system, producing nitric oxide called nitric oxide-plasma activated water (NO-PAW). In this study, we explored the effects of NO-PAW on a cervical cancer cell line, in comparison with DBD plasma. The cytotoxicity results showed that the treatment of HeLa cell with DBD for 4 minutes and 7 μM concentration of NO-PAW could reach almost IC60. For the apoptosis assay, 4 minutes treatment of DBD could induce 7% apoptotic effect, whereas 7 μM NO-PAW could induce 18% apoptotic effect. In addition, we assumed that both DBD plasma and NO-PAW could induce HeLa cell apoptosis by facilitating an accumulation of intracellular reactive oxygen and nitrogen species (RONS). Although further detail on the molecular signal pathway is still needed, DBD and NO-PAW could become promising applications for effective and safe clinical trials for cancer therapy.
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, Korea.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Ho Kang
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, Korea.
authors.forename: Han
authors.initials: H
authors.lastname: Sup Uhm
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, Korea.|Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, Korea.
authors.forename: Geon
authors.initials: G
authors.lastname: Joon Lee
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, Korea.|Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Ha Choi
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, Korea.
authors.forename: Ihn
authors.initials: I
authors.lastname: Han
mesh_terms: D017209:Apoptosis; D001274:Atmospheric Pressure; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006367:HeLa Cells; D006801:Humans; D009569:Nitric Oxide / Q000737:chemistry; D058626:Plasma Gases / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000628:therapy*; D014867:Water / Q000737:chemistry
chemical_list: D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D014867:Water; D009569:Nitric Oxide