title: A DNAzyme Feedback Amplification Strategy for Biosensing.
abstract: We report a signal amplification strategy termed DNAzyme feedback amplification (DFA) that takes advantage of rolling-circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD). DFA employs two specially programmed DNA complexes, one composed of a primer and a circular template containing the antisense sequence of an RCD, and the other composed of the same circular template and an RNA-containing substrate for the RCD. RCA is initiated at the first complex to produce RCD elements that go on to cleave the substrate in the second complex. This cleavage event triggers the production of more input complexes for RCA. This reaction circuit continues autonomously, resulting in exponential DNA amplification. We demonstrate the versatility of this approach for biosensing through the design of DFA systems capable of detecting a microRNA sequence and a bacterium, with sensitivity improvements of 3-6 orders of magnitude over conventional methods.
authors.affiliation: Department of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.|Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
authors.forename: Dingran
authors.initials: D
authors.lastname: Chang
authors.affiliation: Department of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Gu
authors.affiliation: Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: John D
authors.initials: JD
authors.lastname: Brennan
authors.affiliation: Department of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.|Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
authors.forename: Yingfu
authors.identifier: 0000-0002-7533-6743
authors.initials: Y
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D021881:DNA, Catalytic / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D059367:RNA Cleavage
chemical_list: D021881:DNA, Catalytic
keywords: DNAzymes; RNA cleavage; biosensors; feedback amplification; rolling-circle amplification