title: Inhibition of Bacterial Growth by Peptide-Conjugated Morpholino Oligomers.
abstract: Morpholino oligomers (MOs) are antisense molecules designed for sequence-specific binding of target mRNA. In bacteria, inhibition is hypothesized to occur by preventing translation initiation. Cell-penetrating peptides may be conjugated to the 5'- or 3'-termini of an MO to enhance cellular entry and therefore inhibition. Here we describe the three standard microbiological assays to assess in vitro antibacterial MO efficacy.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
authors.forename: Seth M
authors.initials: SM
authors.lastname: Daly
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Sturge
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA. David.Greenberg@UTSouthwestern.edu.|Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA. David.Greenberg@UTSouthwestern.edu.
authors.forename: David E
authors.initials: DE
authors.lastname: Greenberg
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D057846:Cell-Penetrating Peptides / Q000737:chemistry; D004357:Drug Synergism; D008826:Microbial Sensitivity Tests; D060172:Morpholinos / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics*; D010455:Peptides / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D057846:Cell-Penetrating Peptides; D060172:Morpholinos; D016376:Oligonucleotides, Antisense; D010455:Peptides
keywords: Antisense; Checkerboard synergy; Minimum bactericidal concentration (MBC); Minimum inhibitory concentration (MIC); Morpholino oligomers (MOs)