title: Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information.
abstract: Cells communicate with each other to coordinate their gene activities at the population level through signaling pathways. It has been shown that many gene activities are oscillatory and that the frequency and phase of oscillatory gene expression encode various types of information. However, whether or how such oscillatory information is transmitted from cell to cell remains unknown. Here, we developed an integrated approach that combines optogenetic perturbations and single-cell bioluminescence imaging to visualize and reconstitute synchronized oscillatory gene expression in signal-sending and signal-receiving processes. We found that intracellular and intercellular periodic inputs of Notch signaling entrain intrinsic oscillations by frequency tuning and phase shifting at the single-cell level. In this way, the oscillation dynamics are transmitted through Notch signaling, thereby synchronizing the population of oscillators. Thus, this approach enabled us to control and monitor dynamic cell-to-cell transfer of oscillatory information to coordinate gene expression patterns at the population level.
authors.affiliation: Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.|Japan Science and Technology Agency, PRESTO (Precursory Research for Embryonic Science and Technology), Saitama 332-0012, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Isomura
authors.affiliation: Department of Information Sciences, Ochanomizu University, Tokyo 112-8610, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Ogushi
authors.affiliation: Department of Information Sciences, Ochanomizu University, Tokyo 112-8610, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kori
authors.affiliation: Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.|Institute for Integrated Cell-Material Sciences (World Premier International research Center [WPI]-iCeMS), Kyoto University, Kyoto 606-8501, Japan.|Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.|Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Ryoichiro
authors.initials: R
authors.lastname: Kageyama
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D005786:Gene Expression Regulation; D008163:Luminescent Measurements; D051379:Mice; D062308:Optogenetics; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D051880:Receptors, Notch
keywords: Notch signaling; frequency tuning; live imaging; optogenetics; oscillatory expression; phase shifting; synchronization