title: Study of Commercially AvailableLobelia chinensisProducts Using Bar-HRM Technology.
abstract: There is an unmet need for herbal medicine identification using a fast, sensitive, and easy-to-use method that does not require complex infrastructure and well-trained technicians. For instance, the detection of adulterants inLobelia chinensisherbal product has been challenging, since current detection technologies are not effective due to their own limits. High Resolution Melting (HRM) has emerged as a powerful new technology for clinical diagnosis, research in the food industry and in plant molecular biology, and this method has already highlighted the complexity of species identification. In this study, we developed a method of species specific detection ofL. chinensisusing HRM analysis combined with internal transcribed spacer 2. We then applied this method to commercial products purporting to containL.chinensis. Our results demonstrated that HRM can differentiateL. chinensisfrom six common adulterants. HRM was proven to be a fast and accurate technique for testing the authenticity ofL. chinensisin herbal products. Based on these results, a HRM approach for herbal authentication is provided.
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesBeijing, China; Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of SciencesGuangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesBeijing, China; Pharmacy College, Heilongjiang University of Chinese MedicineHarbin, China.
authors.forename: Song
authors.initials: S
authors.lastname: Yan
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesBeijing, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural UniversityGuangzhou, China.
authors.forename: Jingjian
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical SciencesBeijing, China; College of Pharmacy, Hubei University of Chinese MedicineWuhan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.affiliation: Pharmacy College, Heilongjiang University of Chinese Medicine Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing, China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
keywords: Bar-HRM technology; ITS2; Lobelia chinensis; adulterants; herbal medicine identification