title: Tissue distribution and functional analysis of vitellogenin-6 of Toxocara canis.
abstract: Toxocara canis is an common intestinal nematode of canids and the principal causative agent of human toxocariasis. Vitellogenin (Vg), a source of amino acids and lipids in the eggs, are considered to play an important role in embryo development of a wide range of organisms. In the present study, the transcriptional levels of Tc-vit-6 gene in male and female adult T. canis were determined by quantitative real-time PCR, which indicated high transcription of Tc-vit-6 in the intestine, reproductive tract and body wall of male and female adult T. canis. The fragment of Tc-vit-6 encoding a vWD domain, was cloned and expressed to produce a rabbit anti-TcvWD polyclonal antibody. Tissue distribution of TcVg6 was detected by immunohistochemical assays, which showed predominant distribution of TcVg6 in the tissues of intestine, as well as reproductive tract (including some of the germ cells) and musculature of male and female adult worms. Collectively, these results indicated multiple biological roles of TcVg6 apart from that in the reproduction of T. canis.
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: bbaacing@126.com.
authors.forename: Hong-Hong
authors.initials: HH
authors.lastname: Zhu
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: guangxum@student.unimelb.edu.au.
authors.forename: Guang-Xu
authors.initials: GX
authors.lastname: Ma
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: realmyongfang@163.com.
authors.forename: Yong-Fang
authors.initials: YF
authors.lastname: Luo
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: luoyongli21@163.com.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Luo
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: yinshasha23@163.com.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Yin
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: xiongyihqy@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, People's Republic of China. Electronic address: rongqiongzhou@126.com.
authors.forename: Rong-Qiong
authors.initials: RQ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D015153:Blotting, Western; D046028:Canidae / Q000469:parasitology; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation; D005835:Genitalia / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014018:Tissue Distribution; D017166:Toxocara canis / Q000378:metabolism*; D014120:Toxocariasis / Q000469:parasitology*; D014158:Transcription, Genetic; D014819:Vitellogenins / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000502:physiology
chemical_list: D000909:Antibodies, Helminth; D011994:Recombinant Proteins; D014819:Vitellogenins
keywords: Functional analysis; Reproduction; Tissue distribution; Toxocara canis; Transcriptional analysis; Vitellogenin