title: Targeted superparamagnetic nanoparticles coated with 2-deoxy-d-gloucose and doxorubicin more sensitize breast cancer cells to ionizing radiation.
abstract: BACKGROUND
  Nanoparticles are promising as a new approach to enhance chemo- radiotherapy efficiency in breast cancer mainly via targeted therapy.
  
  
  METHODS
  SKBR3 and T47D breast cancer cells were treated with superparamagnetic mesoporous hydroxyapatite nanocomposites (SPmHANs)conjugated with 1 μM doxorubicin and 0.5 mM 2-Deoxy-d-Glucose and irradiated with 1 and 2 Gy gamma rays in vitro. The treatment toxicity and also the apoptosis/necrosis ratio were measured by MTT assay and also ELISA cell death detection PLUS, respectively.
  
  
  RESULTS
  The decreased cell viability with the combined treatment, with determined 42% loading efficiency for 200 ppm 2DG and 93% for5ppm doxorubicin on SPmHANs in PH about 7.4 and 5.5, were calculated to 60.9% and 68% compared to radiotherapy alone inT47D and SKBR3 cells (both with p < 0.05), respectively.
  
  
  CONCLUSIONS
  Breast cancer cure may boost from The combined targeted nanoparticle treatment with doxorubicin and 2-Deoxy-d-Glucose may boost breast cancer radiotherapy by improved chemodrug localization, increased cytotoxicity in tumor cells and decreased single modality treatment doses.
authors.affiliation: Department of Medical Physic, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Pirayesh Islamian
authors.affiliation: Department of Medical Physic, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Milad_Hatamian@yahoo.com.
authors.forename: Milad
authors.initials: M
authors.lastname: Hatamian
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Negar Abbasi
authors.initials: NA
authors.lastname: Aval
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medicinal Chemistry, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Rashidi
authors.affiliation: Department of Medical Physic, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Mesbahi
authors.affiliation: Department of Radiology and Radiotherapy, Imam Reza Medical Center for Treatment and Training, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mohammadzadeh
authors.affiliation: Department of Statistics & Epidemiology, School of Health & Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Asghari Jafarabadi
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001943:Breast Neoplasms / Q000628:therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D059248:Chemoradiotherapy / Q000379:methods; D003131:Combined Modality Therapy; D003847:Deoxyglucose / Q000008:administration & dosage*; D004317:Doxorubicin / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011829:Radiation Dosage; D011839:Radiation, Ionizing
chemical_list: D004317:Doxorubicin; D003847:Deoxyglucose
keywords: 2-Deoxy-d-Glucose; Breast cancer; Chemotherapy; Doxorubicin; Ionizing radiation; Nanocomposite