title: Expression of S100A2 and S100P in human eccrine sweat glands and their application in differentiating secretory coil-like from duct-like structures in the 3D reconstituted eccrine sweat spheroids.
abstract: Secretory coils and ducts are two components of eccrine sweat glands with different structures and functions. In our previous study, we combined keratins and α-SMA to distinguish between secretory coils and ducts. However, the key deficiency of the method was that none of the antibodies used was specific for ducts. In this study, we first examined the co-localization of K5/K7, α-SMA/K14, K7/S100P and α-SMA/S100A2 by double-immunofluorescence staining to confirm the localization of S100P and S100A2 in native human eccrine sweat glands, and second we identified secretory coil-like and duct-like structures in the 3D reconstituted eccrine sweat gland spheroids by double-immunofluorescence staining for K7/S100P and α-SMA/S100A2. In native human eccrine sweat glands, S100A2 immunoreactivity was confined to the outer layer and S100P to the inner layer of the duct. In 12-week Matrigel plugs containing eccrine sweat gland cells, double-immunofluorescence staining for K7/S100P and α-SMA/S100A2 could easily distinguish duct-like structures from secretory coil-like structures. We conclude that S100A2 and S100P can be used as specific duct markers in eccrine sweat glands, and combined use of S100P or S100A2 with keratins enables easy to distinction between secretory coils and ducts.
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China. lihaihong1051@126.com.|Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China. lihaihong1051@126.com.
authors.forename: Haihong
authors.initials: H
authors.lastname: Li
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong Province, China.
authors.forename: Bingna
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Wound Healing and Cell Biology Laboratory, The First Affiliated Hospital, Chinese People's Liberation Army General Hospital, 51 Fucheng Road, Beijing, 100048, China. zcp666666@sohu.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003094:Collagen; D004338:Drug Combinations; D004439:Eccrine Glands / Q000737:chemistry* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins / Q000032:analysis; D007797:Laminin; D009363:Neoplasm Proteins / Q000032:analysis*; D011509:Proteoglycans; D009418:S100 Proteins / Q000032:analysis*; D018874:Spheroids, Cellular / Q000648:ultrastructure*; D055815:Young Adult
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D002135:Calcium-Binding Proteins; D002630:Chemotactic Factors; D004338:Drug Combinations; D007797:Laminin; D009363:Neoplasm Proteins; D011509:Proteoglycans; D009418:S100 Proteins; C098997:S100A2 protein, human; C103003:S100P protein, human; C059485:matrigel; D007633:Keratins; D003094:Collagen
keywords: Duct; Eccrine sweat gland; S100A2; S100P; Secretory coil