title: Supplementation of arachidonic acid rich oil in European sea bass juveniles (Dicentrarchus labrax) diets: Effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels.
abstract: The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax). For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as follows: 0.5% (ARA0.5), 1% (ARA1), 2% (ARA2) and 4% (ARA4) during 60 days. At the end of the feeding trial fatty acid profiles of plasma and head kidney leucocytes were analyzed. Besides, plasma prostaglandins levels, head kidney leucocytes respiratory burst activity; peroxidase activity and phagocytic index were assayed. Reducing dietary ARA levels below 1% markedly reduced European sea bass growth performance. However, fish fed diet ARA0.5 tried to compensate this dietary ARA deficiency by a selective deposition of ARA on plasma and head kidney leucocytes, reaching similar levels to those fish fed diet ARA1 after 60 days of feeding. Nevertheless, head kidney phagocytic capacity was reduced as dietary ARA content in relation not only to variations on membrane composition but also to changes on fish basal prostaglandins levels. Results obtained demonstrated the importance to supply the necessary quantity n-6 LC-PUFA, and not only n-3 LC-PUFA levels, in European sea bass diets, in relation to not only growth performance but also immune system function.
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain. Electronic address: silvia.torrecillas@giaqua.org.
authors.forename: S
authors.initials: S
authors.lastname: Torrecillas
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Román
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Rivero-Ramírez
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Caballero
authors.affiliation: Fisiología e Inmunología de Organismos Marinos. Universidad Nacional Autónoma de México, Unidad Académica Sisal. Puerto de Abrigo, S/N. 97356 Sisal, Hunucma, Yucatán, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Pascual
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Robaina
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: M S
authors.initials: MS
authors.lastname: Izquierdo
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Acosta
authors.affiliation: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Montero
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage / Q000378:metabolism*; D001492:Bass / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000097:blood*; D060226:Head Kidney / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D011453:Prostaglandins / Q000097:blood*; D011897:Random Allocation
chemical_list: D005227:Fatty Acids; D011453:Prostaglandins; D016718:Arachidonic Acid
keywords: Arachidonic acid; European sea bass; Immune system; Leucocytes fatty acid profiles; Plasma prostaglandins