title: Cholinergic and dopaminergic neuronal differentiation of human adipose tissue derived mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types such as cartilage, bone, and fat cells. Recent studies have shown that induction of MSCs in vitro by growth factors including epidermal growth factor (EGF) and fibroblast growth factor (FGF2) causes them to differentiate into neural like cells. These cultures also express ChAT, a cholinergic marker; and TH, a dopaminergic marker for neural cells. To establish a protocol with maximum differentiation potential, we examined MSCs under three experimental culture conditions using neural induction media containing FGF2, EGF, BMP-9, retinoic acid, and heparin. Adipose-derived MSCs were extracted and expanded in vitro for 3 passages after reaching >80% confluency, for a total duration of 9 days. Cells were then characterized by flow cytometry for CD markers as CD44 positive and CD45 negative. MSCs were then treated with neural induction media and were characterized by morphological changes and Q-PCR. Differentiated MSCs expressed markers for immature and mature neurons; β Tubulin III (TUBB3) and MAP2, respectively, showing the neural potential of these cells to differentiate into functional neurons. Improved protocols for MSCs induction will facilitate and ensure the reproducibility and standard production of MSCs for therapeutic applications in neurodegenerative diseases.
authors.affiliation: Biomedical Research Center, Qatar University, Doha, Qatar.
authors.forename: Hany El Sayed
authors.identifier: http://orcid.org/0000-0002-0069-4212
authors.initials: HES
authors.lastname: Marei
authors.affiliation: Faculty of Veterinary Medicine, Department of Cytology and Histology, Mansoura University, Mansoura, Egypt.
authors.forename: Aya
authors.initials: A
authors.lastname: El-Gamal
authors.affiliation: Biomedical Research Center, Qatar University, Doha, Qatar.
authors.forename: Asma
authors.initials: A
authors.lastname: Althani
authors.affiliation: Qatar Biobank, Doha, Qatar.
authors.forename: Nahla
authors.initials: N
authors.lastname: Afifi
authors.affiliation: Faculty of Veterinary Medicine, Department of Cytology and Histology, Mansoura University, Mansoura, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abd-Elmaksoud
authors.affiliation: Faculty of Veterinary Medicine, Department of Cytology and Histology, Mansoura University, Mansoura, Egypt.
authors.forename: Amany
authors.initials: A
authors.lastname: Farag
authors.affiliation: Institute of Translational Pharmacology-CNR, Roma, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Cenciarelli
authors.affiliation: Department of Biomedical Sciences, Virginia Tech Carilion School of Medicine, Roanoke, Virginia.
authors.forename: Caceci
authors.initials: C
authors.lastname: Thomas
authors.affiliation: Department of Mechanical and Industrial Engineering, Qatar University, Doha, Qatar.
authors.forename: Hasan
authors.initials: H
authors.lastname: Anwarul
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D059329:Cholinergic Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D003470:Culture Media / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis / Q000187:drug effects; D010641:Phenotype; D014404:Tubulin / Q000378:metabolism
chemical_list: D003470:Culture Media; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; C498585:TUBB3 protein, human; D014404:Tubulin
keywords: adipose tissue; cholinergic neurons; dopaminergic neurons; mesenchymal stem cell