title: Effects of root debridement and adjunctive photodynamic therapy in residual pockets of patients on supportive periodontal therapy: A randomized split-mouth trial.
abstract: BACKGROUND
  The study aims to compare the effects of adjunctive photodynamic therapy (PDT) with scaling and root debridement alone on periodontal parameters and inflammatory cytokines in residual pockets of patients undergoing maintenance therapy.
  
  
  METHODS
  27 subjects, each with at least 2 residual pockets ≥5mm, were recruited for this randomized, split-mouth controlled trial, providing total of 72 sites. Probing pocket depth (PPD), recession, clinical attachment loss (CAL), plaque and bleeding on probing of all sites were examined at baseline, 3 and 6 months. Gingival crevicular fluids (GCFs) were collected to determine levels of IL-1β, IL-6, IL-8, TNF-α and MMP-8 via enzyme-linked immunosorbent assay. At baseline, all sites received subgingival instrumentation and polishing. In addition, test sites received a single application of PDT using Fotosan®with toluidine blue O solution photosensitizer. At 3 and 6 months, site level analysis was performed for changes in clinical parameters and cytokine level.
  
  
  RESULTS
  Based on mixed model analysis, at 3 months, test sites showed significant reduction in CAL (p=0.016) and PPD (p=0.027) (from 6.14±0.28mm to 5.49±0.20mm and 5.42±0.16mm to 4.65±0.18mm respectively) compared to control sites (from 6.32±0.24mm to 6.08±0.17mm and 5.32±0.13mm to 5.15±0.15mm respectively). At 6 months, these differences were no longer significant (p=0.510). Adjunctive PDT did not offer additional reduction in levels of GCF cytokines.
  
  
  CONCLUSIONS
  A single application of PDT to residual pockets provided a modest improvement of CAL and PPD over 3 months. Application of adjunctive PDT may lead to faster resolution of residual pockets and may be recommended for periodontal patients with slower healing capacity.
authors.affiliation: Discipline of Periodontology, Faculty of Dentistry, National University of Singapore, Republic of Singapore. Electronic address: edwin.goh.x.j@ndcs.com.sg.
authors.forename: Edwin X
authors.initials: EX
authors.lastname: Goh
authors.affiliation: Discipline of Oral Sciences, Faculty of Dentistry, National University of Singapore, Republic of Singapore. Electronic address: kai_soo_tan@nuhs.edu.sg.
authors.forename: Kai Soo
authors.initials: KS
authors.lastname: Tan
authors.affiliation: Biostatistics Unit, Yong Loo Lin School of Medicine, National University of Singapore, Republic of Singapore. Electronic address: yiong_huak_chan@nuhs.edu.sg.
authors.forename: Yiong Huak
authors.initials: YH
authors.lastname: Chan
authors.affiliation: Discipline of Periodontology, Faculty of Dentistry, National University of Singapore, Republic of Singapore. Electronic address: denlimlp@nus.edu.sg.
authors.forename: Lum Peng
authors.initials: LP
authors.lastname: Lim
mesh_terms: D000328:Adult; D000368:Aged; D003131:Combined Modality Therapy / Q000379:methods; D016207:Cytokines / Q000276:immunology; D003646:Debridement / Q000379:methods*; D012534:Dental Scaling / Q000379:methods*; D005260:Female; D005888:Gingival Pocket; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology / Q000473:pathology* / Q000628:therapy*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D014092:Tooth Root / Q000187:drug effects / Q000528:radiation effects; D016896:Treatment Outcome
chemical_list: D016207:Cytokines; D017319:Photosensitizing Agents
keywords: Inflammatory mediators; Periodontal maintenance; Periodontitis; Photodynamic therapy; Supportive periodontal therapy