title: Antiurolithiasis Activity of Bioactivity Guided Fraction ofBergenia ligulataagainst Ethylene Glycol Induced Renal Calculi in Rat.
abstract: Dried rhizome ofBergenia ligulata(pashanbhed) is commonly used as a traditional herbal medicine with a wide range of therapeutic applications including urolithiasis. Aqueous extract ofB. ligulatawas prepared through maceration followed by decoction (mother extract, 35.9% w/w). Further, polarity based fractions were prepared successively from mother extract which yielded 3.4, 2.9, 5.4, 7.5, and 11.3% w/w of hexane, toluene, dichloromethane (DCM),n-butanol, and water fractions, respectively. The in vitro, ex vivo, and real-time antiurolithiasis activity of mother extract and fractions were carried out using aggregation assay in synthetic urine and in rat plasma. The study revealed that DCM fraction has significantly (p< 0.05) greater inhibitory potential than other fractions. Ethylene glycol in drinking water (0.75%, v/v) for 28 days was used for induction of urolithiasis and the curative effects of mother extract and DCM fraction were checked for the level of oxalate, calcium, creatinine, uric acid, and urea of both urine and serum. Treatment with mother extract and DCM fraction at a dose of 185 mg/kg and 7 mg/kg, respectively, in ethylene glycol induced rats resulted in a significant (p< 0.05) decrease in serum and urine markers. Histological study revealed lower number of calcium oxalate deposits with minimum damage in the kidneys of mother extract and DCM fraction treated rats. This result provides a scientific basis for its traditional claims.
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Ikshit
authors.initials: I
authors.lastname: Sharma
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Washim
authors.initials: W
authors.lastname: Khan
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Rabea
authors.initials: R
authors.lastname: Parveen
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Md Javed
authors.initials: MJ
authors.lastname: Alam
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Iftekhar
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Mohd Hafizur Rehman
authors.initials: MH
authors.lastname: Ansari
authors.affiliation: Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Sayeed
authors.identifier: 0000-0003-1573-152X
authors.initials: S
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D004195:Disease Models, Animal; D019855:Ethylene Glycol / Q000633:toxicity; D006801:Humans; D007669:Kidney Calculi / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D031995:Saxifragaceae / Q000737:chemistry*; D052878:Urolithiasis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D019855:Ethylene Glycol