title: SIRT3 functions as a tumor suppressor in hepatocellular carcinoma.
abstract: Hepatocellular carcinoma is one of the leading causes for cancer-related mortality worldwide. SIRT3 may function as either oncogene or tumor suppressor in a panel of cancers; however, the role of SIRT3 in hepatocellular carcinoma remains unclear. In this study, we assayed the expression level of SIRT3 in hepatocellular carcinoma tissues by quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry. A loss-of-function approach was used to examine the effects of SIRT3 on biological activity, including cell proliferative activity and invasive potential. The results demonstrated that the expression levels of SIRT3 protein in hepatocellular carcinoma tissues were significantly downregulated compared with those in adjacent non-cancerous tissues. Furthermore, SIRT3 could decrease cell proliferation and inhibit cell migration/invasion in hepatocellular carcinoma cell line. Taken together, these results elucidated the function of SIRT3 in hepatocellular carcinoma development and suggested that SIRT3 might function as tumor suppressor in hepatocellular carcinoma by targeting PI3K/Akt pathway.
authors.affiliation: 1 Department of Medical Imaging, Guizhou Provincial People's Hospital, Guiyang, China.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Zeng
authors.affiliation: 1 Department of Medical Imaging, Guizhou Provincial People's Hospital, Guiyang, China.
authors.forename: Nanzhu
authors.initials: N
authors.lastname: Wang
authors.affiliation: 2 School of Basic Medicine, Guizhou Medical University, Guiyang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhai
authors.affiliation: 1 Department of Medical Imaging, Guizhou Provincial People's Hospital, Guiyang, China.
authors.forename: Rongpin
authors.initials: R
authors.lastname: Wang
authors.affiliation: 2 School of Basic Medicine, Guizhou Medical University, Guiyang, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Wu
authors.affiliation: 1 Department of Medical Imaging, Guizhou Provincial People's Hospital, Guiyang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Pu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D015536:Down-Regulation; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D056566:Sirtuin 3 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C466093:SIRT3 protein, human; D056566:Sirtuin 3
keywords: AKT; PI3K; SIRT3; hepatocellular carcinoma; oncoprotein