title: Cloacael Carriage and Multidrug ResistanceEscherichia coliO157:H7 from Poultry Farms, Eastern Ethiopia.
abstract: A cross-sectional study was carried out to determine antimicrobial drug resistance patterns ofE. coliO157:H7 isolates and estimate the level of the pathogen. A total of 194 cloacae swab samples were collected randomly in two poultry farms. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolateE. coliO157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of 194 cloacae samples examined, 13.4% (n= 26) were found to be positive forE. coliO157:H7. The finding indicated differences inE. coliO157:H7 infection among the different risk factors. Chicken from Adele Poultry Farm showed higherE. coliO157:H7 infection (OR = 3.89) than Haramaya University poultry farm and young birds had more infection (OR = 4.62) than adult birds. Of the total 14 antimicrobials included in the panel of study, the susceptibility results were varied with 96.15% and 0%E. coliO157:H7 isolates expressing resistance to erythromycin, clindamycin, spectinomycin, and ciprofloxacin, respectively. Multidrug resistance to more than two antimicrobial agents was detected in 24 (92.30%) of the isolates. The study showed high presence of antimicrobial resistant isolates ofE. coliO157:H7. Further study is required to better understand the ecology and evolution of bacterial resistance to antimicrobial agents.
authors.affiliation: School of Veterinary Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia.
authors.forename: Mude
authors.initials: M
authors.lastname: Shecho
authors.affiliation: School of Veterinary Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia.
authors.forename: Naod
authors.identifier: 0000-0002-9616-5015
authors.initials: N
authors.lastname: Thomas
authors.affiliation: College of Veterinary Medicine, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia.
authors.forename: Jelalu
authors.identifier: 0000-0002-1495-2972
authors.initials: J
authors.lastname: Kemal
authors.affiliation: College of Veterinary Medicine, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia.
authors.forename: Yimer
authors.initials: Y
authors.lastname: Muktar