title: Effect of selenylation modification on antitumor activity of peptidoglycan from Lactobacillus acidophilus.
abstract: Selenium is an essential trace element for human with the antitumor properties. In the present study, the peptidoglycan (PG) derived from Lactobacillus acidophilus was modified by selenylation with the HNO3-Na2SeO3method. Reaction temperature, reaction duration and the selenide content were optimized according to orthogonal design of three-factors. In addition, the molecular structure of selenizing peptidoglycan (Se-PG) was determined by infrared spectroscopy analysis. Furthermore, the antitumor activity of Se-PG was also investigated in HT-29 cells. The results showed that Se-PG exerted a greater antitumor activity than non-modified PG in a dose-dependent manner. These findings indicated that selenylation modification can enhance the antitumor activity of PG, and Se-PG could achieve its potential in antitumor activity.
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China. Electronic address: woodsen@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China; Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China. Electronic address: daodongpan@163.com.
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.affiliation: Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002473:Cell Wall; D019073:HT29 Cells; D006801:Humans; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D010457:Peptidoglycan / Q000494:pharmacology*; D012643:Selenium
chemical_list: D000970:Antineoplastic Agents; D010457:Peptidoglycan; D012643:Selenium
keywords: 5-Fluorouracil (PubChem CID:3385); Antitumor activity; Fluorescein isothiocyanate (PubChem CID: 18730); HT-29 cells; Peptidoglycan; Peptidoglycan (PubChem CID:3035036); Propidium iodide (PubChem CID: 104981); Selenylation modification; Trichloroacetic acid (PubChem CID: 6421)