title: Metabolomic analysis and lipid accumulation in a glucose tolerant Crypthecodinium cohnii strain obtained by adaptive laboratory evolution.
abstract: Adaptive laboratory evolution (ALE) was commonly used for strain improvement. Crypthecodinium cohnii is known to accumulate lipids with a high fraction of docosahexaenoic acid (DHA). In order to improve DHA production under high substrate concentration, a glucose-tolerant C. cohnii strain was firstly obtained by ALE after 260 cycles for 650days with gradually increased glucose concentration. The results of lipids content showed that DHA-rich lipids accumulation in the evolved strain could increase by 15.49% at 45g/L glucose concentrations. To reveal mechanisms related to glucose tolerance of C. cohnii through ALE, metabolic profiles were then compared and the results showed that hub metabolites including glycerol, glutamic acid, malonic acid and succinic acid were positively regulated during ALE. The study demonstrated that metabolomic analysis complemented with ALE could be an effective and valuable strategy for basic mechanisms of molecular evolution and adaptive changes in C. cohnii.
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China.
authors.forename: Xingrui
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Pei
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China.
authors.forename: Liangsen
authors.initials: L
authors.lastname: Liu
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China. Electronic address: lchen@tju.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science & Engineering, Tianjin, PR China; Center for Biosafety Research and Strategy, Tianjin University, Tianjin, PR China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D004141:Dinoflagellida / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D050356:Lipid Metabolism; D055432:Metabolomics
chemical_list: D004281:Docosahexaenoic Acids; D005947:Glucose
keywords: Adaptive laboratory evolution; C. cohnii; Glucose tolerance; Lipid accumulation; Metabolomic analysis