title: Combining Patient-Reprogrammed Neural Cells and Proteomics as a Model to Study Psychiatric Disorders.
abstract: The mechanisms underlying the pathophysiology of psychiatric disorders are still poorly known. Most of the studies about these disorders have been conducted on postmortem tissue or in limited preclinical models. The development of human induced pluripotent stem cells (iPSCs) has helped to increase the translational capacity of molecular profiling studies of psychiatric disorders through provision of human neuronal-like tissue. This approach consists of generation of pluripotent cells by genetically reprogramming somatic cells to produce the multiple neural cell types as observed within the nervous tissue. The finding that iPSCs can recapitulate the phenotype of the donor also affords the possibility of using this approach to study both the disease and control states in a given medical area. Here, we present a protocol for differentiation of human pluripotent stem cells to neural progenitor cells followed by subcellular fractionation which allows the study of specific cellular organelles and proteomic analysis.
authors.affiliation: Lab of Neuroproetomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil.
authors.forename: Giuliana S
authors.initials: GS
authors.lastname: Zuccoli
authors.affiliation: Lab of Neuroproetomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil.|UNICAMP's Neurobiology Center, Campinas, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.affiliation: Lab of Neuroproetomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Institute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, 21941-901, Brazil.|D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, 22281-100, Brazil.
authors.forename: Stevens K
authors.initials: SK
authors.lastname: Rehen
authors.affiliation: Lab of Neuroproetomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil. juminardi@gmail.com.|D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, 22281-100, Brazil. juminardi@gmail.com.
authors.forename: Juliana Minardi
authors.initials: JM
authors.lastname: Nascimento
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002478:Cells, Cultured; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D053595:Embryonic Stem Cells / Q000737:chemistry*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000737:chemistry*; D001523:Mental Disorders / Q000378:metabolism* / Q000473:pathology; D036103:Nanotechnology / Q000379:methods; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000302:isolation & purification; D058953:Neural Stem Cells / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D009419:Nerve Tissue Proteins
keywords: Brain; Differentiation; Mass spectrometry; Neuronal precursor cell; Proteomics; Psychiatric disorders; iPSC