title: Mind Bomb-Binding Partner RanBP9 Plays a Contributory Role in Retinal Development.
abstract: Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression ofranbp9in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown ofranbp9resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation markerhuC, elevation of neural proliferation markerher4, and alteration of cell cycle markerp57kip2. Expression of the Müller glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bomb-binding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Kyeong-Won
authors.initials: KW
authors.lastname: Yoo
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Maivannan
authors.initials: M
authors.lastname: Thiruvarangan
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Jeong
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Sateesh
authors.initials: S
authors.lastname: Maddirevula
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Myungchull
authors.initials: M
authors.lastname: Rhee
authors.affiliation: Comparative Biomedicine Research Branch, Research Institute, National Cancer Center, Goyang 10408, Korea.
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Bae
authors.affiliation: Department of OB/GYN, Department of Neuroscience and Regenerative Medicine, Augusta University, GA 30912, USA.
authors.forename: Hyung-Goo
authors.initials: HG
authors.lastname: Kim
authors.affiliation: Department of Biology, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Kim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D019556:COS Cells; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D000067778:ELAV-Like Protein 3 / Q000235:genetics / Q000378:metabolism; D063928:Ependymoglial Cells / Q000502:physiology; D055785:Gene Knockdown Techniques; D055495:Neurogenesis / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D015792:Retinal Dysplasia / Q000235:genetics; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051792:Basic Helix-Loop-Helix Proteins; D050761:Cyclin-Dependent Kinase Inhibitor p57; D003598:Cytoskeletal Proteins; D000067778:ELAV-Like Protein 3; D009687:Nuclear Proteins; D044767:Ubiquitin-Protein Ligases; D029961:Zebrafish Proteins; C116005:Ran binding protein 9; C088635:elavl3 protein, zebrafish; C119433:her4.1 protein, zebrafish; C471932:mib1 protein, zebrafish
keywords: RanBP9; mind bomb; notch; retinogenesis; zebrafish