title: ACE I/D Polymorphism in Hypertensive Patients of Kashmiri Population.
abstract: BACKGROUND
  The angiotensin-converting enzyme (ACE) gene in humans has an insertion-deletion (I/D) polymorphic state in intron 16 on chromosome 17q23. This polymorphism has been widely investigated in different diseases. In this study we aimed to investigate the ACE I/D genotype frequency in hypertensive cases in Kashmiri population.
  
  
  METHODS
  We designed a case control study, where 52 hypertensive cases were studied for ACE I/D polymorphism against 150 age/sex matched controls taken from general population. The polymorphisms of ACE gene were investigated using polymerase chain reaction for detection of ACE I/D genotype. Fisher's Chi square test was used for calculation of P value and OR.
  
  
  RESULTS
  We found the frequency of ACE DD genotype to be 46.15% (24/52), II 23.07% (12/52) and DI 30.77% (16/52) in 52 hypertensive cases.
  
  
  CONCLUSIONS
  The ACE I/D genotype is positively associated with hypertension in our population.
authors.affiliation: Departments of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190011, India; Departments of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: A Syed
authors.initials: AS
authors.lastname: Sameer
authors.affiliation: Departments of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190011, India.
authors.forename: Nidda
authors.initials: N
authors.lastname: Syeed
authors.affiliation: Departments of Cardiology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
authors.forename: Shahid A
authors.initials: SA
authors.lastname: Tak
authors.affiliation: Department of Clinical Biochemistry, Kashmri University, Hazratbal, Srinagar, Kashmir, India.
authors.forename: Samina
authors.initials: S
authors.lastname: Bashir
authors.affiliation: Departments of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190011, India; Department of Clinical Biochemistry, Kashmri University, Hazratbal, Srinagar, Kashmir, India.
authors.forename: Saniya
authors.initials: S
authors.lastname: Nissar
authors.affiliation: Departments of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190011, India.
authors.forename: Mushtaq A
authors.initials: MA
authors.lastname: Siddiqi
keywords: ACE; Hypertension; Kashmir; Polymorphism; RFLP