title: FBP1 is highly expressed in human hypertrophic scars and increases fibroblast proliferation, apoptosis, and collagen expression.
abstract: FBP1, one of the far-upstream element binding proteins(FBPs), is a distal upstream binding protein of c-myc, which is highly expressed in tumor tissues. This study aimed to investigate FBP1 expression in human hypertrophic scars and to determine the effects of FBP1 on fibroblasts.
  
  
  
  Human normal skin and scar specimens were collected during clinical surgery. One portion of each tissue specimen was embedded in paraffin and sliced to observe differences in histological features and FBP1 expression by immunohistochemistry and western blotting. The other portion of each tissue specimen was cultured to obtain fibroblasts. Fibroblasts from the second to the sixth passage were used for the experiments, which were divided into the following two groups: an experimental group, whose cells were transfected with an siRNA targeting FBP1, and a control group, whose cells where not transfected. MTT and TUNEL assays were performed, respectively, to assess fibroblast proliferation and apoptosis, and western blotting was performed to assess protein expression.
  
  
  
  We obtained fibroblasts by primary tissue culture and found that FBP1 was highly expressed in hypertrophic scars. MTT assay showed that an siRNA targeting FBP1 significantly reduced fibroblast proliferation in siRNA-treated cells compared to control cells. TUNEL assay showed that there was no difference in apoptosis between the two groups; however, western blotting showed that collagen I, collagen III, c-myc, caspase-3, and caspase-9 expression levels were all decreased in the experimental group.
  
  
  
  FBP1 is highly expressed in human hypertrophic scars and increases fibroblast proliferation, apoptosis and collagen expression.
authors.affiliation: a Department of Graduate School , Southern Medical University , Guangzhou , China.|b Department of Trauma and Microsurgery , Guangdong No. 2 People's Hospital , Guangzhou , China.
authors.forename: Jieyi
authors.initials: J
authors.lastname: Zuo
authors.affiliation: b Department of Trauma and Microsurgery , Guangdong No. 2 People's Hospital , Guangzhou , China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Chen
authors.affiliation: c Guangzhou Exon Biotechnology Co., Ltd , Guangzhou , China.
authors.forename: Xinchao
authors.initials: X
authors.lastname: Zhong
authors.affiliation: b Department of Trauma and Microsurgery , Guangdong No. 2 People's Hospital , Guangzhou , China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Lan
authors.affiliation: b Department of Trauma and Microsurgery , Guangdong No. 2 People's Hospital , Guangzhou , China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Kuang
authors.affiliation: a Department of Graduate School , Southern Medical University , Guangzhou , China.|b Department of Trauma and Microsurgery , Guangdong No. 2 People's Hospital , Guangzhou , China.
authors.forename: Dong
authors.initials: D
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004265:DNA Helicases / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D016601:RNA-Binding Proteins
chemical_list: D004268:DNA-Binding Proteins; C492258:FUBP1 protein, human; D016601:RNA-Binding Proteins; D003094:Collagen; D004265:DNA Helicases
keywords: Apoptosis; C-myc; FBP1; fibroblast; hypertrophic scar; proliferation