title: Pharmacokinetic study of representative anti-oxidative compounds from Denshen-Chuanxiong-Honghua following oral administration in rats.
abstract: Almost no pharmacokinetic compounds to date have been precisely linked with the activity of their herbal or Traditional Chinese Medicine (TCM) formula. This creates challenges for pharmacokinetic significance and application of the TCM. In our study, a sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to quantitatively or qualitatively determine multiple-components (tanshinol, ferulic acid, protocatechuic acid, rosmarinic acid, salvianolic acid B, baicalin and 9'-methyl lithospermate B) in rat plasma following the oral administration of Denshen-Chuanxiong-Honghua (DCH) extract (20g/kg). Chromatographic separation was carried out on a 300SB-C18 column using a gradient elution with a mobile phase composed of acetonitrile-water (containing 0.1% formic acid) at a flow rate of 1.0mL/min. Determination by mass spectrometry (MS) was conducted in multiple reaction monitoring (MRM) mode with negative electrospray ionization. The validated method exhibited good linearity, with correlation coefficients greater than 0.9949 over a wide concentration range, and the lower limits of quantification were 2.09-12.2ng/mL for the 5 analytes. This assay was successfully applied to investigate the pharmacokinetics of 5 compounds in rat plasma after the oral administration of DCH extracts. In addition, the anti-oxidant capacities of the 5 active ingredients of DCH extract in vitro and the total absorbed DCH extract in vivo were investigated at different concentrations during pharmacokinetic studies.
authors.affiliation: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, PR China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, PR China.
authors.forename: Xianhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Tcm-related Comorbid Depression, Nanjing University of Traditional Chinese Medicine, Nanjing 210064, PR China.
authors.forename: Wan
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Neurology, Binzhou Central Hospital, Binzhou Medical College, Binzhou 251700, PR China.
authors.forename: Huali
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of Tcm-related Comorbid Depression, Nanjing University of Traditional Chinese Medicine, Nanjing 210064, PR China. Electronic address: huangx59@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.affiliation: Institute of Tcm-related Comorbid Depression, Nanjing University of Traditional Chinese Medicine, Nanjing 210064, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.affiliation: School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zou
authors.affiliation: School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, 720 Rutland Ave, Ross Bldg 370B, Baltimore, MD 21205, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, Thomas Jefferson University,1025 Walnut Street, 808 College Building, Philadelphia, PA 19107, USA.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Ma
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics*; D031187:Carthamus tinctorius / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C079426:Honghua extract, Carthamus tinctorius
keywords: Anti-oxidative; DCH extract; Herb; LC/MS/MS; Pharmacokinetic