title: Cationic Phosphorus Dendrimer Enhances Photodynamic Activity of Rose Bengal against Basal Cell Carcinoma Cell Lines.
abstract: In the last couple of decades, photodynamic therapy emerged as a useful tool in the treatment of basal cell carcinoma. However, it still meets limitations due to unfavorable properties of photosensitizers such as poor solubility or lack of selectivity. Dendrimers, polymers widely studied in biomedical field, may play a role as photosensitizer carriers and improve the efficacy of photodynamic treatment. Here, we describe the evaluation of an electrostatic complex of cationic phosphorus dendrimer and rose bengal in such aspects as singlet oxygen production, cellular uptake, and phototoxicity against three basal cell carcinoma cell lines. Rose bengal-cationic dendrimer complex in molar ratio 5:1 was compared to free rose bengal. Obtained results showed that the singlet oxygen production in aqueous medium was significantly higher for the complex than for free rose bengal. The cellular uptake of the complex was 2-7-fold higher compared to a free photosensitizer. Importantly, rose bengal, rose bengal-dendrimer complex, and dendrimer itself showed no dark toxicity against all three cell lines. Moreover, we observed that phototoxicity of the complex was remarkably enhanced presumably due to high cellular uptake. On the basis of the obtained results, we conclude that rose bengal-cationic dendrimer complex has a potential in photodynamic treatment of basal cell carcinoma.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz , Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Dabrzalska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz , Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Janaszewska
authors.affiliation: Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Sienkiewicza 112, 90-363 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Zablocka
authors.affiliation: Laboratoire de Chimie et de Biochimie pharmacologiques et toxicologique, Université Paris Descartes, PRES Sorbonne Paris Cité, CNRS UMR 860 , 45 rue des Saints Pères, 75006 Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mignani
authors.affiliation: Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077 Toulouse, France.|Université de Toulouse, UPS, INPT , 31077 Toulouse Cedex 4, France.
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Majoral
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz , Pomorska 141/143, 90-236 Lodz, Poland.|Leibniz-Institut für Polymerforschung Dresden e.V. , Hohe Strasse 6, 01069 Dresden, Germany.
authors.forename: Barbara
authors.identifier: 0000-0003-3459-8947
authors.initials: B
authors.lastname: Klajnert-Maculewicz
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry*; D051379:Mice; D010758:Phosphorus / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D012395:Rose Bengal / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D012395:Rose Bengal; D010758:Phosphorus
keywords: basal cell carcinoma; phosphorus dendrimer; photodynamic therapy; photosensitizer; phototoxicity; rose bengal