title: Double strand break repair and γ-H2AX formation in erythrocytes of medaka (Oryzias latipes) after γ-irradiation.
abstract: The study of the DNA damage response in erythrocytes after γ-irradiation may provide evidence for its effectiveness as a biomarkers for genotoxic environmental stress. We previously reported various malformations in erythrocytes of medaka irradiated with10 Gy, but not in their micronuclei. In this study, we optimized an assay method for γ-H2AX and double strand breaks in erythrocytes of adult medaka fish after 15 Gy of γ-irradiation. The highest level of apoptosis and nuclear abnormalities, including in micronuclei, were recorded 4 h after γ-irradiation, as was the highest level of γ-H2AX foci in erythrocytes. These results suggest that recognition and repair processes occur as a response to DNA damage in erythrocytes in medaka.
authors.affiliation: Zoology Department, Faculty of Science, Assiut University, 71516, Assiut, Egypt; Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan. Electronic address: alaa_h254@yahoo.com.
authors.forename: Alaa El-Din Hamid
authors.initials: AEH
authors.lastname: Sayed
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Watanabe-Asaka
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitani
mesh_terms: D000818:Animals; D017209:Apoptosis; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D004912:Erythrocytes / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D006657:Histones / Q000528:radiation effects*; D009990:Oryzias / Q000235:genetics*
chemical_list: D006657:Histones
keywords: DNA repair; Erythrocytes; Fish; Radiation; γ-H2AX