title: NMR solution structure of the RED subdomain of the Sleeping Beauty transposase.
abstract: DNA transposons can be employed for stable gene transfer in vertebrates. The Sleeping Beauty (SB) DNA transposon has been recently adapted for human application and is being evaluated in clinical trials, however its molecular mechanism is not clear. SB transposition is catalyzed by the transposase enzyme, which is a multi-domain protein containing the catalytic and the DNA-binding domains. The DNA-binding domain of the SB transposase contains two structurally independent subdomains, PAI and RED. Recently, the structures of the catalytic domain and the PAI subdomain have been determined, however no structural information on the RED subdomain and its interactions with DNA has been available. Here, we used NMR spectroscopy to determine the solution structure of the RED subdomain and characterize its interactions with the transposon DNA.
authors.affiliation: Department of Physics and Optical Science, University of North Carolina, Charlotte, North Carolina, 28223.
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Konnova
authors.affiliation: Department of Physics and Optical Science, University of North Carolina, Charlotte, North Carolina, 28223.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Singer
authors.affiliation: Department of Physics and Optical Science, University of North Carolina, Charlotte, North Carolina, 28223.|Center for Biomedical Engineering and Science, University of North Carolina, Charlotte, North Carolina, 28223.
authors.forename: Irina V
authors.identifier: 0000-0002-1835-1533
authors.initials: IV
authors.lastname: Nesmelova
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D004251:DNA Transposable Elements; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D019895:Transposases / Q000737:chemistry*
chemical_list: D004251:DNA Transposable Elements; D004247:DNA; D019895:Transposases; C494471:sleeping beauty transposase, human
keywords: DNA binding; NMR spectroscopy; RED subdomain; SB transposase; folding; structure