title: Ultrasensitive electrochemical biosensing platform based on spherical silicon dioxide/molybdenum selenide nanohybrids and triggered Hybridization Chain Reaction.
abstract: An ultrasensitive sandwich-type electrochemical biosensor for DNA detection is developed based on spherical silicon dioxide/molybdenum selenide (SiO2@MoSe2) and graphene oxide-gold nanoparticles (GO-AuNPs) hybrids as carrier triggered Hybridization Chain Reaction (HCR) coupling with multi-signal amplification. The proposed sensoring assay utilizes a spherical SiO2@MoSe2/AuNPs as sensing platform and GO-AuNPs hybrids as carriers to supply vast binding sites. H2O2+HQ system is used for DNA detection and HCR as the signal and selectivity enhancer. The sensor is designed in sandwich type to increase the specificity. As a result, the present biosensor exhibits a good dynamic range from 0.1fM to 100pM with a low detection limit of 0.068fM (S/N=3). This work shows a considerable potential for quantitative detection of DNA in early clinical diagnostics.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Shuai
authors.affiliation: School of Physics and Electronic Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China. Electronic address: kejinghuang@163.com.
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Huang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.
authors.forename: Zi-Bo
authors.initials: ZB
authors.lastname: Zhai
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008982:Molybdenum / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D012822:Silicon Dioxide; D006108:Graphite; D008982:Molybdenum; D004247:DNA
keywords: Electrochemical biosensor; Graphene oxide–gold nanoparticles; Hybridization Chain Reaction; Sandwich-type; Signal amplification; Spherical silicon dioxide/molybdenum selenide