title: Newcastle disease virus-like particles induce dendritic cell maturation and enhance viral-specific immune response.
abstract: Circulating of genotype VII Newcastle disease virus (NDV) is a great threat to the poultry industry worldwide. Virus-like particles (VLPs) are increasingly being considered as potential viral vaccines due to their safety and efficacy. In this study, we analyzed in vitro the stimulatory effects of VLPs containing the matrix and hemagglutinin-neuraminidase of genotype VII NDV on dendritic cells (DCs) and evaluated their immunogenicity in mice. The results showed that immature bone marrow-derived dendritic cells (BMDCs) responded to stimulation with VLPs by up-regulating expressions of MHC II, CD40, CD80, and CD86 molecules and by increasing the cytokine secretions of TNF-α, IFN-γ, IL-6, and IL-12p70. Besides, VLPs enhanced the immunostimulatory capacity of DCs to stimulate autologous T cell proliferation. Furthermore, VLPs can induce efficient humoral and cellular immune responses, and recruit mature DCs to the spleen in C57BL/6 mice, as shown by an obvious increase in double-positive proliferation of splenic CD11c+CD86+ cells. These data indicate that NDV VLPs can be a valuable candidate for NDV vaccine development.
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Ding
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China.
authors.forename: Renfu
authors.initials: R
authors.lastname: Yin
authors.affiliation: Engineering Research Center of Jilin Province for Animals Probiotics, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Yixue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Xue
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xu
authors.affiliation: Engineering Research Center of Jilin Province for Animals Probiotics, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
authors.affiliation: Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Shunlin
authors.initials: S
authors.lastname: Hu
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China. congyanlong@126.com.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China. congyanlong@126.com.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun, 130062, China. dingzhuang@jlu.edu.cn.|Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun, 130062, China. dingzhuang@jlu.edu.cn.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000821:virology; D049109:Cell Proliferation; D002645:Chickens; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000821:virology; D005260:Female; D005777:Geese; D007111:Immunity, Cellular; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009521:Newcastle Disease / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
keywords: Dendritic cell; Immune response; Newcastle disease virus; Virus-like particle