title: Neuroprotective properties of Madecassoside from Centella asiatica after hypoxic-ischemic injury.
abstract: Madecassoside is one of increasingly used constituent of Centella asiatica, a frequently prescribed crude drug in South eastern Asia and China for wound healing. In the present experiment, it exposes the neuroprotective nature of Madecassoside in GT1-7 cell lines, further, which the antioxidant activities are performed. The cellular toxicity was assessed using 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay with increased cell viability with IC502.5µg/ml. the regulation of antioxidant levels showed changes in madecassoside treated cell lysate viz., SOD assay. Also, the antioxidative assays confirmed the negligible cellular damage caused to the GT1-7 cell lines. Hence, the results advocate that the current antioxidant and antitumor activity be justified by the high concentration of phenolic constituents, primarily the triterpene present in the C. asiatica.
authors.affiliation: Department of Neurology, Qian Foshan Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Shu Qing
authors.initials: SQ
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Shandong Provincial Corps Hospital of Chinese People's Armed Police Force, Shandong Province, China.
authors.forename: Yong Sheng
authors.initials: YS
authors.lastname: Xie
authors.affiliation: Department of Neurology, Shandong Police General Hospital, Shandong Province, China.
authors.forename: Qing Wen
authors.initials: QW
authors.lastname: Meng
authors.affiliation: Department of Neurology, Qian Foshan Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Qian Foshan Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D028041:Centella / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts; D010946:Plants, Medicinal; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; C526146:Centella asiatica extract; D018696:Neuroprotective Agents; D010851:Picrates; D010936:Plant Extracts; D014315:Triterpenes; C093443:madecassoside; C004931:1,1-diphenyl-2-picrylhydrazyl; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase