title: Cytochrome c oxidase subunit I haplotype diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae).
abstract: The rat lungworm Angiostrongylus cantonensis is a food-borne zoonotic parasite of public health importance worldwide. It is the primary etiologic agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans in many countries. It is highly endemic in Thailand especially in the northeast region. In this study, A. cantonensis adult worms recovered from the lungs of wild rats in different geographical regions/provinces in Thailand were used to determine their haplotype by means of the mitochondrial partial cytochrome c oxidase subunit I (COI) gene sequence. The results revealed three additional COI haplotypes of A. cantonensis. The geographical isolates of A. cantonensis from Thailand and other countries formed a monophyletic clade distinct from the closely related A. malaysiensis. In the present study, distinct haplotypes were identified in seven regions of Thailand - AC10 in Phitsanulok (northern region), AC11 in Nakhon Phanom (northeastern region), AC15 in Trat (eastern region), AC16 in Chantaburi (eastern region), AC4 in Samut Prakan (central region), AC14 in Kanchanaburi (western region), and AC13 in Ranong (southern region). Phylogenetic analysis revealed that these haplotypes formed distinct lineages. In general, the COI sequences did not differentiate the worldwide geographical isolates of A. cantonensis. This study has further confirmed the presence of COI haplotype diversity in various geographical isolates of A. cantonensis. The COI gene sequence will be a suitable marker for studying population structure, phylogeography and genetic diversity of the rat lungworm.
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University - Bangkok 10700, Thailand. Electronic address: praphathip.eam@mahidol.ac.th.
authors.forename: Praphathip
authors.initials: P
authors.lastname: Eamsobhana
authors.affiliation: Institute of Ocean and Earth Sciences, University of Malaya - 50603, Kuala Lumpur, Malaysia.
authors.forename: Sze-Looi
authors.initials: SL
authors.lastname: Song
authors.affiliation: Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Hoi-Sen
authors.initials: HS
authors.lastname: Yong
authors.affiliation: Department of Disease Control, Ministry of Public Health, Nonthaburi, 11000, Thailand.
authors.forename: Anchana
authors.initials: A
authors.lastname: Prasartvit
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University - Bangkok 10700, Thailand.
authors.forename: Sudarat
authors.initials: S
authors.lastname: Boonyong
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University - Bangkok 10700, Thailand. Electronic address: anchalee.tun@mahidol.ac.th.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tungtrongchitr
mesh_terms: D017185:Angiostrongylus cantonensis / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation; D006239:Haplotypes; D010802:Phylogeny; D058974:Phylogeography; D051381:Rats; D012376:Rodent Diseases / Q000453:epidemiology / Q000469:parasitology*; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary*; D013785:Thailand
chemical_list: D003576:Electron Transport Complex IV
keywords: Angiostrongyliasis; Angiostrongylus cantonensis; Genetic diversity; Haplotype; Phylogeography; Rat lungworm