title: Aflatoxin B1 Contamination of Dairy Feeds after Storage in Farm Practice in Tropical Environmen.
abstract: The objective of this study was to determine the contamination of aflatoxin B1 (AF-B1) when keeping various dairy feeds in a farm environment. The study was carried out from March to May 2011 and involved 63 small holder dairy farms belonging to a single dairy cooperative in Chiang Mai province, Thailand. All feed samples used for milking cows including 4 commercial concentrates (CC1 to CC4), by-products from local corn processing factories fermented in plastic bags (SIL), and corn and cob meal or corn dust (CCD). Feed samples were collected 2 times at before and after storage. Farmers were requested to store CC1 to CC4 and CCD for a month and SIL for a week using their routine on-farm storage arrangements. All samples were measured for their AF-B1 concentrations by ELISA. Results showed that AF-B1 concentrations of CC1 to CC4, SIL and CCD before storage were 5.1, 4.1, 4.0, 4.2, 5.5 and 5.5 μg/kg, respectively, and after storage the concentration of AF-B1 were 9.7, 6.5, 9.8, 12.3, 11.4 and 20.0 μg/kg, respectively. CCD at after storage was the only feed that had mean level more than 20 μg/kg. Concentrations of AF- B1 at before storage in all feeds were significantly lower than after storage (P<0.01), and the increased ratio of AF-B1 levels was approximately 2 to 3 times. The study concluded that increased AF-B1 levels are related to feed types and farm conditions.
authors.affiliation: Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University.
authors.forename: Wantanwa
authors.initials: W
authors.lastname: Mongkon
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
authors.forename: Wasana
authors.initials: W
authors.lastname: Chaisri
authors.forename: Witaya
authors.initials: W
authors.lastname: Suriyasathaporn
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000818:Animals; D003611:Dairy Products / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D000072480:Farms; D005506:Food Contamination; D005516:Food Microbiology; D061353:Food Storage; D014329:Tropical Climate
chemical_list: D016604:Aflatoxin B1