title: Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay.
abstract: Escherichia coliO157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detectEscherichia coliO157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; aCitrobacterfreundiistrain was isolated from the ground pork samples and identified by using CHROmagarTMplates, API 20E, and 16S RNA sequencing. SinceC.freundiishowed cross-reactivity withE. coliO157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection ofE. coliO157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized forE. coliO157:H7 enrichment andC.freundiiinhibition by the addition of 5 mg/L potassium tellurite and 0.10 mg/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU/gC.freundiidecreased to 5%.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. 15797769982@163.com.
authors.forename: Song
authors.initials: S
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. minghuiai@sina.com.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. joezgg@sina.com.
authors.forename: Gang-Gang
authors.initials: GG
authors.lastname: Zhang
authors.affiliation: Department of Science and Technology, Nanchang University, Nanchang 330047, China. yzb1156@126.com.
authors.forename: Zhi-Biao
authors.initials: ZB
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. defoelau@163.com.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. xiongyonghua@ncu.edu.cn.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. weihua@ncu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. laiweihua@ncu.edu.cn.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Lai
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D019453:Escherichia coli O157; D005516:Food Microbiology; D006046:Gold; D007118:Immunoassay; D053768:Metal Nanoparticles; D000069466:Red Meat; D013552:Swine
chemical_list: D006046:Gold
keywords: E. coli O157:H7; enrichment; false positive; ground pork sample