title: Comprehensive metabonomic analysis of heart tissue from isoproterenol-induced myocardial infarction rat based on reversed-phase and hydrophilic interaction chromatography coupled to mass spectrometry.
abstract: We aim to describe the metabonomic characteristics of myocardial infarction rats. High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was utilized to develop a metabonomic method of the heart homogenates of myocardial infarction rats. Hydrophilic interaction chromatography allows the analysis of high polar metabolites, providing complementary information to reversed-phase liquid chromatography. We combined reversed phase and hydrophilic interaction chromatographic separations to analyze 18 samples, ten from myocardial infarction rat hearts and eight from normal rat hearts. A total of 16 potential biomarkers in rat heart tissue were screened out, primarily related to oxidative stress, nitric oxide damage, taurine, and hypotaurine metabolism and sphingolipid metabolism. This research showed that a comprehensive metabonomic study is a useful tool to reveal the underlying mechanism of myocardial infarction.
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.|Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.
authors.forename: Jianxun
authors.identifier: http://orcid.org/0000-0003-4028-8427
authors.initials: J
authors.lastname: Liu
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.
authors.forename: Mingqian
authors.initials: M
authors.lastname: Sun
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Miao
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Xiyuan hospital, China Academy of Chinese Medical Sciences and Beijing key lab of TCM pharmacology, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006321:Heart / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D007545:Isoproterenol; D013058:Mass Spectrometry; D055432:Metabolomics; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats
chemical_list: D007545:Isoproterenol
keywords: biomarkers; hydrophilic interaction chromatography; metabonomics; myocardial infarction; reversed-phase chromatography