title: Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis.
abstract: Lymph node metastasis (LNM) is common in papillary thyroid cancer (PTC), and is an indicator of recurrence. The detailed molecular mechanism of LNM in PTC has not been well described. This study aimed to investigate the role of fibronectin 1 in PTC LNM and its clinical relevance. The expression of fibronectin 1 was confirmed in PTC tissues and cell lines. A correlation analysis was conducted and a receiver-operating characteristic curve obtained. The effect of fibronectin 1 on the proliferation of PTC cell lines was performed using a colony-formation assay and Cell Counting Kit 8. Cell-cycle analysis was performed with a flow-cytometry assay. Migration and invasion ability were evaluated by transwell and wound-healing assays. Fibronectin 1 was overexpressed in metastasized PTC. Overexpressed fibronectin 1 was positively correlated with PTC LNM. Receiver-operating characteristic analysis showed that the diagnostic accuracy of fibronectin 1 was 81.1%, with sensitivity of 80% and specificity of 82%. Overexpression of fibronectin 1 promoted proliferation, migration, and invasion in PTC. Fibronectin 1 plays a critical role in PTC metastasis by modulating the proliferation, migration, and invasion ability of PTC cells, and it is a valuable diagnostic biomarker for predicting PTC LNM.
authors.affiliation: Ultrasound Department, RuiJin Hospital, Shanghai Jiao Tong University School of Medicine.
authors.forename: Shujun
authors.initials: S
authors.lastname: Xia
authors.affiliation: Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) and Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai, China.
authors.forename: Chuandong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Surgery, University Medical Center Utrecht, Utrecht, the Netherlands.
authors.forename: Emily Louise
authors.initials: EL
authors.lastname: Postma
authors.affiliation: Ultrasound Department, RuiJin Hospital, Shanghai Jiao Tong University School of Medicine.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Ultrasound Department, RuiJin Hospital, Shanghai Jiao Tong University School of Medicine.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ni
authors.affiliation: Ultrasound Department, RuiJin Hospital, Shanghai Jiao Tong University School of Medicine.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhan
keywords: diagnostic biomarker; fibronectin 1; lymph node metastasis; papillary thyroid cancer