title: Embryonic yolk removal affects neither morphology nor escape performance of larval axolotls.
abstract: Maternal effects, the influences of maternal phenotype on the phenotypes of her offspring, mediate early ontogenetic traits through maternal investment. In amphibians, provisioning eggs with yolk is the main source of maternal investment. While larger eggs generally result in larger, higher-quality offspring, the relationship between egg size and offspring phenotype is complicated because offspring can evolve to be more or less responsive to variation in yolk provisions. Previous studies of several ambystomatid salamanders suggest that the effects of embryonic yolk reserve reduction on hatchling life history traits increase with egg size. In this study, a similar controlled experimental yolk removal technique in Ambystoma mexicanum was used to determine the effects of reduced yolk reserves on phenotypes including hatching time and stage, hatchling and larval size and performance in predation trials with fish. Surprisingly, yolk reduction revealed no effects on any traits. These findings suggest that larval morphology in A. mexicanum is highly canalized and larval phenotypes are decoupled from yolk reserve variation. This surprising lack of yolk removal effects in hatchling and larval axolotls illustrates the evolutionary flexibility of early life history traits. Traits can evolve to increase or decrease their response to resources and can even become completely unresponsive. Since we found no effects in early life history, we hypothesize that domestication of the axolotl may have altered yolk properties or allocation dynamics and that maternal investment in yolk reserves may manifest at later life stages by reducing the time to reproductive maturity or increasing fecundity.
authors.affiliation: Arcadia University, 450 South Easton Road, Glenside, PA 19038, USA. Electronic address: cbrain0114@gmail.com.
authors.forename: Charity
authors.initials: C
authors.lastname: Brain
authors.affiliation: Arcadia University, 450 South Easton Road, Glenside, PA 19038, USA. Electronic address: landbergt@arcadia.edu.
authors.forename: Tobias
authors.initials: T
authors.lastname: Landberg
mesh_terms: D000558:Ambystoma mexicanum / Q000196:embryology*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000502:physiology*; D004924:Escape Reaction / Q000502:physiology*; D007814:Larva / Q000254:growth & development / Q000502:physiology; D015017:Yolk Sac
keywords: Allometric engineering; Developmental plasticity; Egg size/number trade-off; Invasive tilapia