title: Effects of Dietary Resistant Starch on the Wnt Signaling Pathway and Preneoplastic Cells in the Colons of Azoxymethane-Treated Rats.
abstract: Dietary resistant starch (RS) has been suggested to reduce colonic neoplasia. To determine the effects of digestion-resistant cornstarch on colonic carcinogenesis and Wnt signaling in azoxymethane (AOM)-treated F344 rats, diets containing naturally occurring RS from corn lines derived partially from Guat209 (GUAT), AR16035 (AR), or a hybrid (ARxGUAT), containing 34.5 ± 2.0, 0.2 ± 0.1, and 1.9 ± 0.1% RS, respectively, were fed at 55% of the diet. GUAT-fed rats had increased cecal content and tissue weight and decreased cecal pH compared with AR- or ARxGUAT-fed rats. Numbers of aberrant crypt foci (ACF) were not different among diet groups. Increased numbers of crypts/focus were observed in AOM-injected rats fed GUAT compared with rats fed other diets. β-catenin mRNA expression of the crypts was significantly increased in GUAT-fed rats injected with AOM relative to those injected with saline. These findings suggest that selected dietary RSs may at some level further enhance colonocyte proliferation and differentiation in an AOM-treated colon.
authors.affiliation: a Department of Veterinary Pathology , Iowa State University , Ames , Iowa , USA.|b Interdepartmental Graduate Program in Genetics , Iowa State University , Ames , Iowa , USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Cray
authors.affiliation: b Interdepartmental Graduate Program in Genetics , Iowa State University , Ames , Iowa , USA.|c Department of Food Science and Human Nutrition , Iowa State University , Ames , Iowa , USA.
authors.forename: Yinsheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: d Department of Statistics , Iowa State University , Ames , Iowa , USA.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: d Department of Statistics , Iowa State University , Ames , Iowa , USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: e USDA-Agricultural Research Service , Washington, DC , USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Pollak
authors.affiliation: e USDA-Agricultural Research Service , Washington, DC , USA.|f Department of Agronomy (retired) , Iowa State University , Ames , Iowa , USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Duvick
authors.affiliation: b Interdepartmental Graduate Program in Genetics , Iowa State University , Ames , Iowa , USA.|c Department of Food Science and Human Nutrition , Iowa State University , Ames , Iowa , USA.
authors.forename: Diane F
authors.initials: DF
authors.lastname: Birt
authors.affiliation: a Department of Veterinary Pathology , Iowa State University , Ames , Iowa , USA.|b Interdepartmental Graduate Program in Genetics , Iowa State University , Ames , Iowa , USA.|g Pathogenesis , LLC , Gainesville , Florida , USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Whitley
mesh_terms: D058739:Aberrant Crypt Foci / Q000178:diet therapy / Q000473:pathology; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity; D001835:Body Weight / Q000187:drug effects; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000178:diet therapy / Q000473:pathology; D004032:Diet; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D011230:Precancerous Conditions / Q000178:diet therapy* / Q000378:metabolism; D011916:Rats, Inbred F344; D013213:Starch / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000187:drug effects* / Q000235:genetics; D003313:Zea mays / Q000737:chemistry / Q000235:genetics
chemical_list: D013213:Starch; D001397:Azoxymethane