title: DNA nanopore functionalized with aptamer and cell-penetrating peptide for tumor cell recognition.
abstract: In the present work, DNA nanopore was composed of a bundle of six DNA duplexes folded from six DNA strands and functionalized with Ramos cell aptamer and cell-penetrating peptide (CPP). Herein, we present a unique dually conjugated molecule with an aptamer and cell-penetrating peptide for targeting and recognition of Ramos cells. The aptamer sequence was specific bound to Ramos cell, at the meanwhile the nanopore assembly was taken onto the surface of cell membrane and then got into the cells with the help of CPP. Specific targeting and increased intracellular uptake of nanostructures by Ramos cells were aimed. The intracellular uptake of the structure was determined using confocal microscopy. This study is the first to describe the recognition of tumor cells with functional DNA nanopores, establishing the foundation for the tumor cell detection with low cytotoxic agents. Graphical abstract DNA nanopore was composed of a bundle of six DNA duplexes folded from six DNA strands and functionalized with Ramos cell aptamer and cell-penetrating peptide. The dually conjugated molecule was found to show both improved cellular uptake and effective Ramos cell targeting.
authors.affiliation: Center of Cooperative Innovation for Chemical Imaging Functional Probes in Universities of Shandong, College of Chemistry, Shandong Normal University, Jinan, Shandong, 250014, China.|Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers Research Institute of Biochemical Analysis, Linyi University, Linyi, Shandong, 276005, China.
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Guo
authors.affiliation: Center of Cooperative Innovation for Chemical Imaging Functional Probes in Universities of Shandong, College of Chemistry, Shandong Normal University, Jinan, Shandong, 250014, China.|Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers Research Institute of Biochemical Analysis, Linyi University, Linyi, Shandong, 276005, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Yuan
authors.affiliation: Center of Cooperative Innovation for Chemical Imaging Functional Probes in Universities of Shandong, College of Chemistry, Shandong Normal University, Jinan, Shandong, 250014, China.|Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers Research Institute of Biochemical Analysis, Linyi University, Linyi, Shandong, 276005, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Song
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers Research Institute of Biochemical Analysis, Linyi University, Linyi, Shandong, 276005, China. xuemei_li@yeah.net.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Li
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D058608:Nanopores / Q000648:ultrastructure; D009369:Neoplasms / Q000000981:diagnostic imaging*; D061848:Optical Imaging / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D057846:Cell-Penetrating Peptides; D004247:DNA
keywords: Aptamer; Cell-penetrating peptide; DNA nanopore; Ramos cell