title: Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms.
abstract: The unique properties of vesicular stomatitis virus (VSV) make it a promising vaccine platform. With the advent of plasmid-based approaches to generate recombinant VSV viruses that express glycoproteins of other viruses, researchers now have the means to generate vaccine candidates targeting a variety of human pathogens. This chapter gives a general overview of the workings of VSV as a vaccine platform and provides a detailed protocol for the generation of recombinant VSV from plasmids.
authors.affiliation: Department of Microbiology and National Emerging Infectious Disease Laboratory, Boston University School of Medicine, Boston, MA, USA.
authors.forename: John B
authors.initials: JB
authors.lastname: Ruedas
authors.affiliation: Department of Microbiology and National Emerging Infectious Disease Laboratory, Boston University School of Medicine, Boston, MA, USA. jhconnor@bu.edu.
authors.forename: John H
authors.initials: JH
authors.lastname: Connor
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D059386:Reverse Genetics; D014616:Vaccinia virus / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
keywords: Reverse genetics; T7 RNA polymerase; Vaccine platform; Vaccinia virus; Vesicular stomatitis virus