title: L-type amino acid transporter 1 expression in esophageal carcinogenesis according to WHO and Japanese classifications of intraepithelial neoplasia.
abstract: L-type amino acid transporter 1 (LAT1) has an essential role in cell proliferation especially in neoplasms. Although immunohistochemical expression of LAT1 has been investigated in invasive esophageal carcinoma, its expression in intraepithelial neoplasia (IEN) has not been reported. Further, classification of esophageal IEN is currently different between the World Health Organization (WHO) and Japanese criteria. Therefore, in this study, immunohistochemical expressions of LAT1 along with Ki-67 were analyzed in 66 esophageal samples of endoscopic submucosal dissection. Extension of cells positive for either marker within the epithelium, along with LAT1 intensity at the base of the epithelium, was evaluated. The results among early IENs, progressed IENs, and invasive carcinoma based on both WHO and Japanese criteria were compared. It was demonstrated that Ki-67+cells extended toward the superficial layer in IENs, which was more pronounced in progressed compared with early IENs based on both WHO and Japanese criteria. Although similar results were obtained for LAT1+cells, LAT1+cell extended more in invasive carcinoma than in progressed IENs according to the WHO criteria. Further, LAT1 intensity was different between early and progressed IENs based on the Japanese criteria alone. Thus, use of LAT1 immunohistochemistry and Japanese classification may be more meaningful to characterize esophageal carcinogenesis.
authors.affiliation: Department of Diagnostic Pathology, NTT Medical Center Tokyo.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Hashimoto
authors.affiliation: Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurata
authors.affiliation: Department of Preventive Medicine and Public Health, Tokyo Medical University.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kikuchi
authors.affiliation: Department of Diagnostic Pathology, NTT Medical Center Tokyo.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Fujita
authors.affiliation: Department of Laboratory Medicine, NTT Medical Center Tokyo.
authors.forename: Rikiya
authors.initials: R
authors.lastname: Okuyama
authors.affiliation: Department of Preventive Medicine and Public Health, Tokyo Medical University.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Department of Diagnostic Pathology, NTT Medical Center Tokyo.|Department of Laboratory Medicine, NTT Medical Center Tokyo.
authors.forename: Hajime
authors.initials: H
authors.lastname: Horiuchi
authors.affiliation: Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kuroda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D063646:Carcinogenesis; D002278:Carcinoma in Situ / Q000145:classification* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D004938:Esophageal Neoplasms / Q000145:classification* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014944:World Health Organization
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D027283:Large Neutral Amino Acid-Transporter 1
keywords: Ki-67; L-type amino acid transporter 1; classification; esophageal intraepithelial neoplasia; immunohistochemistry