title: The Antigen-Presenting Potential of Vγ9Vδ2 T Cells During Plasmodium falciparum Blood-Stage Infection.
abstract: During Plasmodium falciparum infections, erythrocyte-stage parasites inhibit dendritic cell maturation and function, compromising effective antimalarial adaptive immunity. Human Vγ9Vδ2 T cells can act in vitro as antigen-presenting cells (APCs) and induce αβ T-cell activation. However, the relevance of this activity in vivo has remained elusive. Because Vγ9Vδ2 T cells are activated during the early immune response against P. falciparum infection, we investigated whether they could contribute to the instruction of adaptive immune responses toward malaria parasites. In P. falciparum-infected patients, Vγ9Vδ2 T cells presented increased surface expression of APC-associated markers HLA-DR and CD86. In response to infected red blood cells in vitro, Vγ9Vδ2 T cells upregulated surface expression of HLA-DR, HLA-ABC, CD40, CD80, CD83, and CD86, induced naive αβ T-cell responses, and cross- presented soluble prototypical protein to antigen-specific CD8+ T cells. Our findings qualify Vγ9Vδ2 T cells as alternative APCs, which could be harnessed for therapeutic interventions and vaccine design.
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Howard
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.
authors.forename: Séverine
authors.initials: S
authors.lastname: Loizon
authors.affiliation: Division of Infection and Immunity, School of Medicine, and.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Tyler
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Duluc
authors.affiliation: Division of Infection and Immunity, School of Medicine, and.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Moser
authors.affiliation: Interdepartmental Section Tropical Medicine and Clinical International Health, Division of Infectious and Tropical Diseases, Department of Medicine, University Hospital Centre, Bordeaux.|INSERM 897 & Centre René-Labusquière (Tropical Medicine Branch), Faculty of Medicine, University of Bordeaux.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Mechain
authors.affiliation: Interdepartmental Section Tropical Medicine and Clinical International Health, Division of Infectious and Tropical Diseases, Department of Medicine, University Hospital Centre, Bordeaux.|INSERM 897 & Centre René-Labusquière (Tropical Medicine Branch), Faculty of Medicine, University of Bordeaux.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Duvignaud
authors.affiliation: Interdepartmental Section Tropical Medicine and Clinical International Health, Division of Infectious and Tropical Diseases, Department of Medicine, University Hospital Centre, Bordeaux.|INSERM 897 & Centre René-Labusquière (Tropical Medicine Branch), Faculty of Medicine, University of Bordeaux.
authors.forename: Denis
authors.initials: D
authors.lastname: Malvy
authors.affiliation: Department of Molecular Biosciences, Wenner-Gren Institute, Stockholm University, Sweden.
authors.forename: Marita
authors.initials: M
authors.lastname: Troye-Blomberg
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.|CHU de Bordeaux, Immunology and Immunogenetic Laboratory, and.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Moreau
authors.affiliation: Division of Infection and Immunity, School of Medicine, and.|Systems Immunity Research Institute, Cardiff University, United Kingdom ; and.
authors.forename: Matthias
authors.initials: M
authors.lastname: Eberl
authors.affiliation: Department of Parasites and Insect Vectors, Institut Pasteur,Paris, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Mercereau-Puijalon
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.
authors.forename: Julie
authors.initials: J
authors.lastname: Déchanet-Merville
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Behr
authors.affiliation: ImmunoConcEpt, CNRS UMR 5164, Bordeaux University.
authors.forename: Maria
authors.initials: M
authors.lastname: Mamani-Matsuda
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology*; D010641:Phenotype; D010963:Plasmodium falciparum / Q000276:immunology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
keywords: Plasmodium falciparum infection.; antigen presentation; malaria; γδ T cells