title: Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis.
abstract: Substances with valuable antitumor properties have been identified in many marine algae, including an edible polysaccharide from the marine algaGracilariopsis lemaneiformis(PGL). We previously reported transcriptome profiling data showing that PGL induced transcriptional alterations generate anti-lung cancer activity. To identify how PGL is detrimental to tumors, we purified PGL to characterize its chemical composition, molecular weight, and sugar and protein content and investigated its antitumor activity. We demonstrated that PGL exerted its antitumor activities by modulating cell viability, morphology, apoptosis, and the apoptosis-related Fas/FasL signaling pathway in the human lung cancer cell line A549, the gastric cancer cell line MKN28, and the mouse melanoma cell line B16. Our data provide the first evidence that PGL inhibits cell proliferation by inducing apoptosis, which is largely mediated by Fas/FasL in cancer cells, suggesting that PGL might be a novel therapeutic agent against cancer.
authors.affiliation: Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China. kangyani@sjtu.edu.cn.|School of Biomedical Engineering, Bio-ID Research Center, Shanghai Jiao Tong University, Shanghai 200240, China. kangyani@sjtu.edu.cn.
authors.forename: Yani
authors.initials: Y
authors.lastname: Kang
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China. wangzj1978@126.com.
authors.forename: Zhi-Jiang
authors.initials: ZJ
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. dshxie@sjtu.edu.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Xie
authors.affiliation: Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China. sunxue@nbu.edu.cn.
authors.forename: Xue
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China. yangwenge@nbu.edu.cn.
authors.forename: Wenge
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Biomedical Engineering, Bio-ID Research Center, Shanghai Jiao Tong University, Shanghai 200240, China. xiaodongzhao@sjtu.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China. xunianjun@nbu.edu.cn.
authors.forename: Nianjun
authors.initials: N
authors.lastname: Xu
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D053222:Fas Ligand Protein; D011134:Polysaccharides
keywords: Fas/FasL pathway; Gracilariopsis lemaneiformis; antitumor activity; apoptosis; cell proliferation; polysaccharide