title: Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi.
abstract: Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining using the EriG protein sequence as a probe led to the discovery of a new family of ubiquitous UbiA-related diterpene cyclases in bacteria and fungi. We successfully characterized seven new diterpene cyclases from bacteria or basidiomycete fungi with the help of an engineered Escherichia coli strain and determined the structures of their corresponding products. A new diterpene with an unusual skeleton was generated during this process. The discovery of this new family of diterpene cyclases provides new insight into the UbiA superfamily.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Microbial, Physiological, and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Microbial Resources, Yunnan Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
authors.forename: Shunxing
authors.initials: S
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Wenzhao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Microbial, Physiological, and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Hongwei
authors.identifier: 0000-0001-6471-131X
authors.initials: H
authors.lastname: Liu
mesh_terms: D000363:Agaricales / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001487:Basidiomycota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D003500:Cyclization; D004122:Dimethylallyltranstransferase / Q000235:genetics / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D005810:Multigene Family; D010802:Phylogeny
chemical_list: D004224:Diterpenes; C497675:cyathane; D004122:Dimethylallyltranstransferase
keywords: UbiA; biosynthesis; cyclases; diterpenes; natural products