title: Expression of the Antioxidative Enzyme Peroxiredoxin 2 in Multiple Sclerosis Lesions in Relation to Inflammation.
abstract: Multiple sclerosis is a chronic inflammatory disease of the central nervous system, characterized by demyelination and axonal damage as well as neuronal degeneration. Since oxygen-derived free radicals are an important factor leading to tissue damage in inflammatory multiple sclerosis (MS) lesions, research on antioxidative systems is essential to identify endogenous factors which can possibly counteract oxidative damage. As an important scavenging enzyme family, peroxiredoxins (PRDXs) play a crucial role in preventing oxidative damage; however little is known about their expression and function in MS lesions. In the present study we examined the expression of PRDX2 in white matter lesions of MS patients with long-standing, chronic disease. PRDX2 expression was investigated by immunohistochemistry in the context of oxidative stress and inflammation (determined by microglia/macrophage and T cell infiltration) in ten MS autopsy cases as well as seven control autopsy cases. PRDX2 was found to be upregulated in white matter MS lesions mainly in astrocytes, and its expression level was positively correlated with the degree of inflammation and oxidative stress. Our data suggest that PRDX2 expression contributes to the resistance of astrocytes against oxidative damage.
authors.affiliation: Institute of Neuropathology, University Medical Center, Robert-Koch-Straße 40, Göttingen 37075, Germany. david-voigt@gmx.net.
authors.forename: David
authors.initials: D
authors.lastname: Voigt
authors.affiliation: Institute of Neuropathology, University Medical Center, Robert-Koch-Straße 40, Göttingen 37075, Germany. uscheidt@med.uni-goettingen.de.
authors.forename: Uta
authors.initials: U
authors.lastname: Scheidt
authors.affiliation: Neurologic Clinic and Policlinic, University Hospital Basel, Basel 4031, Switzerland. tobias.derfuss@usb.ch.
authors.forename: Tobias
authors.initials: T
authors.lastname: Derfuss
authors.affiliation: Institute of Neuropathology, University Medical Center, Robert-Koch-Straße 40, Göttingen 37075, Germany. wbrueck@med.uni-goettingen.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brück
authors.affiliation: Institute of Neuropathology, University Hospital Essen, Hufelandstr. 55, Essen 45122, Germany. andreas.junker@uk-essen.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Junker
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001344:Autopsy; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D066127:White Matter / Q000378:metabolism* / Q000473:pathology
chemical_list: D054464:Peroxiredoxins; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human
keywords: NAD(P)H quinone dehydrogenase 1 (NQO1); inflammation; multiple sclerosis (MS); oxidative stress; peroxiredoxin 2 (PRDX2)