title: Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies-Synthesis and Characterization.
abstract: The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.
authors.affiliation: Faculty of Science and Letters, Department of Biology, Aksaray University, Aksaray 68100,Turkey. gamzetan2003@yahoo.com.|Philipp University of Marburg, Marburg 35001, Germany. gamzetan2003@yahoo.com.
authors.forename: Gamze
authors.initials: G
authors.lastname: Tan
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. karsten.kantner@physik.uni-marburg.de.
authors.forename: Karsten
authors.initials: K
authors.lastname: Kantner
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. qian.zhang@physik.uni-marburg.de.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. mahmoud.gamalsoliman@physik.uni-marburg.de.
authors.forename: Mahmoud G
authors.initials: MG
authors.lastname: Soliman
authors.affiliation: Centro de Investigación Cooperativa Biomagune, San Sebastián 20001, Spain. pdelpino@cicbiomagune.es.
authors.forename: Pablo
authors.initials: P
authors.lastname: Del Pino
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. pdelpino@cicbiomagune.es.|Centro de Investigación Cooperativa Biomagune, San Sebastián 20001, Spain. pdelpino@cicbiomagune.es.
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
authors.affiliation: Faculty of Science, Department of Biology, Hacettepe University, Ankara 06800, Turkey. mali@hacettepe.edu.tr.
authors.forename: Mehmet A
authors.initials: MA
authors.lastname: Onur
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. daniel.valdepereztoledo@physik.uni-marburg.de.
authors.forename: Daniel
authors.initials: D
authors.lastname: Valdeperez
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. j_rejman@hotmail.com.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rejman
authors.affiliation: Philipp University of Marburg, Marburg 35001, Germany. beatriz.pelazgarcia@physik.uni-marburg.de.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pelaz
keywords: VEGF; bioconjugation; cellular uptake; gold nanoparticles; nanoparticle characterization; nanoparticle-cell interaction; toxicity