title: Contents variation analysis of free amino acids, nucleosides and nucleobases in semen sojae praeparatum fermentation using UFLC-QTRAP MS.
abstract: UFLC-QTRAP MS was used to develop a sensitive and rapid method of evaluating content variation during Semen sojae praeparatum (SSP) fermentation. It did this through the simultaneous quantification of 22 free amino acids and 16 nucleosides and nucleobases in the raw materials and processed products of SSP. The method was shown to be reproducible and accurate. The limits of detection and quantity values were 0.09-168.75 and 0.31-562.50 ng/mL for the 38 analytes, respectively. The data were examined through principal components analysis to compare the content variations. The quantitative results showed that the ingredients were properly determined in most of the samples and were converted regularly throughout the SSP fermentation process. These results correspond to the morphologic changes and principal components analysis results.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chai
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Chenxiao
authors.initials: C
authors.lastname: Shan
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Hongmei
authors.identifier: http://orcid.org/0000-0003-1226-759X
authors.initials: H
authors.lastname: Wen
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D009705:Nucleosides / Q000032:analysis*; D015203:Reproducibility of Results; D045730:Soy Foods / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D009705:Nucleosides
keywords: UFLC-QTRAP MS; fermentation; free amino acids; nucleosides and nucleobases; principal component analysis; semen sojae praeparatum