title: Single step biotransformation of corn oil phytosterols to boldenone by a newly isolatedPseudomonas aeruginosa.
abstract: A new potentPseudomonas aeruginosaisolate capable for biotransformation of corn oil phytosterol (PS) to 4-androstene-3, 17-dione (AD), testosterone (T) and boldenone (BOL) was identified by phenotypic analysis and 16S rRNA gene sequencing. Sequential statistical strategy was used to optimize the biotransformation process mainly concerning BOL using Factorial design and response surface methodology (RSM). The production of BOL in single step microbial biotransformation from corn oil phytosterols byP. aeruginosawas not previously reported. Results showed that the pH concentration of the medium, (NH4)2SO4and KH2PO4were the most significant factors affecting BOL production. By analyzing the statistical model of three-dimensional surface plot, BOL production increased from 36.8% to 42.4% after the first step of optimization, and the overall biotransformation increased to 51.9%. After applying the second step of the sequential statistical strategy BOL production increased to 53.6%, and the overall biotransformation increased to 91.9% using the following optimized medium composition (g/l distilled water) (NH4)2SO4, 2; KH2PO4, 4; Na2HPO4. 1; MgSO4·7H2O, 0.3; NaCl, 0.1; CaCl2·2H2O, 0.1; FeSO4·7H2O, 0.001; ammonium acetate 0.001; Tween 80, 0.05%; corn oil 0.5%; 8-hydroxyquinoline 0.016; pH 8; 200 rpm agitation speed and incubation time 36 h at 30 °C. Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Al Azhar University, Assiut, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Eisa
authors.affiliation: Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt.
authors.forename: Heba
authors.initials: H
authors.lastname: El-Refai
authors.affiliation: Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Egypt.
authors.forename: Magdy
authors.initials: M
authors.lastname: Amin
keywords: Androstenedione; Boldenone; Corn oil phytosterols; Factorial design; Pseudomonads aeruginosa