title: HOTAIR upregulates an 18-gene cell cycle-related mRNA network in glioma.
abstract: HOTAIR is a tumor promoting long non-coding RNA (lncRNA) with roles in multiple cancers. However, the role of HOTAIR in glioma has not been well charaterized. Genes that positively correlated with HOTAIR were identified from the Chinese Glioma Genome Atlas and constructed into an interacting network. In total, 18 genes with P-values <0.01 were further extracted and constructed into a subnetwork. Real-time PCR, western blot and immunofluorescence analyses were employed to examine the expression of the genes after HOTAIR overexpression or knockdown. Intracranial glioblastoma multiform (GBM) models were used to test the potential of HOTAIR as a glioma therapy target. It was discovered that the 18 genes that most significantly correlated with HOTAIR expression formed a cell cycle-related mRNA network, which is positively regulated by HOTAIR. Furthermore, HOTAIR knockdown inhibited mouse intracranial GBM model formation. HOTAIR positively regulates a cell cycle-related mRNA network in glioma, and could be a potential therapeutic target for treating glioma.
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.affiliation: Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Bingcong
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Kang
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Qixue
authors.initials: Q
authors.lastname: Wang