title: BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage.
abstract: Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but the exact mechanism underlying this is not yet clearly understood. Here, using coimmunoprecipitation (Co-IP) and immunofluorescence methods, we prove that Bcl-2-associated athanogene 5 (BAG5), a BAG family member, interacts with DJ-1 in mammalian cells. Moreover, we show that BAG5 could decrease stability of DJ-1 and weaken its role in mitochondrial protection probably by influencing dimerization in stress condition. Our study reveals the relationship of BAG5 and DJ-1 suggesting a potential role for BAG5 in the pathogenesis of PD through its functional interactions with DJ-1.
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Li-Xia
authors.identifier: 0000-0002-3050-3181
authors.initials: LX
authors.lastname: Qin
authors.affiliation: The State Key Laboratory of Medical Genetics and School of Life Science, Central South University, Changsha, Hunan, China.
authors.forename: Jie-Qiong
authors.initials: JQ
authors.lastname: Tan
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Hai-Nan
authors.initials: HN
authors.lastname: Zhang
authors.affiliation: Department of Biology, Allama Iqbal Open University, Islamabad, Pakistan.
authors.forename: Kousar
authors.initials: K
authors.lastname: Rizwana
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Lu
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Tang
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xiang-Min
authors.initials: XM
authors.lastname: Shen
authors.affiliation: The State Key Laboratory of Medical Genetics and School of Life Science, Central South University, Changsha, Hunan, China; Department of Neurology, Xiangya Hospital, Central South University, Changsha, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, China; Neurodegenerative Disorders Research Center, Central South University, Changsha, China.
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Guo
authors.affiliation: The State Key Laboratory of Medical Genetics and School of Life Science, Central South University, Changsha, Hunan, China; Department of Neurology, Xiangya Hospital, Central South University, Changsha, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, China; Neurodegenerative Disorders Research Center, Central South University, Changsha, China.
authors.forename: Bei-Sha
authors.initials: BS
authors.lastname: Tang
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Tan
authors.affiliation: Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Chun-Yu
authors.identifier: 0000-0003-1629-0885
authors.initials: CY
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D057809:HEK293 Cells; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism*; D018384:Oxidative Stress; D000071617:Protein Deglycase DJ-1 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C496515:BAG5 protein, human; D018840:HSP70 Heat-Shock Proteins; D018696:Neuroprotective Agents; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D012402:Rotenone; D000071617:Protein Deglycase DJ-1