title: Highly sensitive microcantilever-based immunosensor for the detection of carbofuran in soil and vegetable samples.
abstract: Microcantilever-based immunosensor is a next-generation electromechanical technique with broad application in biological detection. In this paper, we reported a microcantilever-based immunosensor that quantitatively detect the carbofuran, by using monoclonal antibodies to carbofuran as the receptor molecules. The surface of gold-coated microcantilever was chemically modified by the crosslinking of l-cysteine (l-cys)/glutaraldehyde (GA). The monoclonal antibodies to carbofuran were then immobilized on the side of the microcantilever to fabricate the immunosensor, the mechanical bending induced by antigen-antibody specific binding under an experimental environment. Under the optimized conditions, immunosensor detected carbofuran showed a good linear relationship over the range from 1.0×10-7to 1.0×10-3g/L (R=0.998), with a detection limit of 0.1ng/mL. Moreover, the proposed immunosensor exhibited high sensitivity, specificity and good stability and can be successfully applied in the carbofuran determination in soil and vegetable samples with satisfactory results.
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou 225002, China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Dai
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou 225002, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou 225002, China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Environmental Science & Engineering, Yangzhou University, 196 Hua-Yang-Xi Road, Yangzhou 225002, China.
authors.forename: Shuzhao
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Taizhou Aimo Medical Science and Technology Development Limited Company, Taizhou 225300, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou 225002, China. Electronic address: wangcy@yzu.edu.cn.
authors.forename: Chengyin
authors.initials: C
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques / Q000379:methods*; D002235:Carbofuran / Q000032:analysis* / Q000276:immunology; D003545:Cysteine; D005976:Glutaral; D006046:Gold; D007118:Immunoassay / Q000379:methods*; D012680:Sensitivity and Specificity; D012987:Soil / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D012987:Soil; D006046:Gold; D003545:Cysteine; D002235:Carbofuran; D005976:Glutaral
keywords: Atomic force microscope; Carbofuran; Immunosensor; Microcantilever