title: Improved xylitol production by expressing a novel d-arabitol dehydrogenase from isolated Gluconobacter sp. JX-05 and co-biotransformation of whole cells.
abstract: In the present study, a novel ardh gene encoding d-arabitol dehydrogenase (ArDH) was cloned and expressed in Escherichia coli from a new isolated strain of Gluconobacter sp. JX-05. Sequence analysis revealed that ArDH containing a NAD(P)-binding motif and a classical active site motif belongs to the short-chain dehydrogenase family. Subsequently, the optimal pH and temperature, specific activities and kinetic parameter of ArDH were determined. In the co-biotransformation by the whole cells of BL21-ardh and BL21-xdh, 26.1g/L xylitol was produced from 30g/L d-arabitol in 22h with a yield of 0.87g/g. The xylitol production was increased by more than two times as compared with that of Gluconobacter sp. alone, and was improved 10.1% than that of Gluconobacter sp. mixed with BL21-xdh.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China. Electronic address: qxh@ujs.edu.cn.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Qi
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Tinashe Archbold
authors.initials: TA
authors.lastname: Magocha
authors.affiliation: College of Biosciences and Biotechnology, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110161, Liaoning, China.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: An
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Yun
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Yang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Nanning Bioclone Biotechnology Co., Ltd, 5 Keyuan Road, Nanning 530001, Guangxi, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Liang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles 611 Charles E. Young Dr. E, Los Angeles 90095, CA, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cao
mesh_terms: D001711:Biotransformation; D050542:D-Xylulose Reductase / Q000235:genetics; D020576:Gluconobacter / Q000378:metabolism*; D010088:Oxidoreductases; D014993:Xylitol / Q000096:biosynthesis*
chemical_list: D010088:Oxidoreductases; D050542:D-Xylulose Reductase; D014993:Xylitol
keywords: Biotransformation; Characteristic; Gluconobacter sp.; Whole cell; d-Arabitol dehydrogenase