title: Exosomes Isolated from Ascites of T-Cell Lymphoma-Bearing Mice Expressing Surface CD24 and HSP-90 Induce a Tumor-Specific Immune Response.
abstract: Extracellular vesicles (EVs), including endosome-derived nanovesicles (exosomes), are involved in cell-cell communication. Through transfer of their molecular contents, extracellular nanovesicles can alter the function of recipient cells. Due to these characteristics, EVs have shown potential as a new alternative for cancer immunotherapy. Tumor exosomes isolated from malignant ascites can activate dendritic cells, thereby priming the immune system to recognize and kill cancer cells. However, a suppressive role on tumor immune response has also been reported, suggesting that the neoplastic stage of carcinogenesis and the microenvironment where tumor cells grow may influence the amount of EVs released by the cell. This neoplastic stage and microenvironment may also impact EVs' components such as proteins and miRNA, determining their biological behavior. Most T-cell lymphomas have an aggressive clinical course and poor prognosis. Consequently, complementary alternative therapies are needed to improve the survival rates achieved with conventional treatments. In this work, we have characterized EVs isolated from ascites of mice bearing a very aggressive murine T-cell lymphoma and have studied their immunogenic properties. Small EVs were isolated by differential centrifugation, ultrafiltration, and ultracentrifugation at 100,000 ×gon a sucrose cushion. The EVs were defined as exosomes by their morphology and size analyzed by electron microscopy, their floating density on a sucrose gradient, as well as their expression of endosome marker proteins ALIX, TSG-101; the tetraspanins CD63, CD9, and CD81. In addition, they contain tumor antigens, the marker for malignancy CD24, the heat shock protein HSP-70, and an unusual surface expression of HSP-90 was demonstrated. The administration of EVs isolated from ascites (EVs A) into naïve-syngeneic mice induced both humoral and cellular immune responses that allowed the rejection of subsequent tumor challenges. However, the immunization had no effect on a non-related mammary adenocarcinoma, demonstrating that the immune response elicited was specific and also it induced immune memory.In vitroanalysis demonstrated that T-cells from EVs A-immunized mice secrete IFN-γ in response to tumor stimulation. Furthermore, tumor-specific CD4+ and CD8+ IFN-γ secreting cells could be efficiently expanded from mice immunized with EVs A, showing that a T helper 1 response is involved in tumor rejection. Our findings confirm exosomes as promising defined acellular tumor antigens for the development of an antitumor vaccine.
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Menay
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Leticia
authors.initials: L
authors.lastname: Herschlik
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Julieta
authors.initials: J
authors.lastname: De Toro
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Federico
authors.initials: F
authors.lastname: Cocozza
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Tsacalian
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA) , Buenos Aires , Argentina.
authors.forename: María José
authors.initials: MJ
authors.lastname: Gravisaco
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: María Paula
authors.initials: MP
authors.lastname: Di Sciullo
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Alejandrina
authors.initials: A
authors.lastname: Vendrell
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Claudia I
authors.initials: CI
authors.lastname: Waldner
authors.affiliation: Centro de Estudios Farmacológicos y Botánicos-Consejo Nacional de Investigaciones Científicas y Técnicas (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, Argentina.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mongini
keywords: T-cell lymphoma; ascites; exosomes; immune response; tumor vaccine