title: The Construction and Identification of Induced Pluripotent Stem Cells Derived from Acute Myelogenous Leukemia Cells.
abstract: OBJECTIVE
  The present study aimed to establish an induced pluripotent stem cell (iPSC) line from acute myelogenous leukemia (AML) cells in vitro and identify their biological characteristics.
  
  
  METHODS
  Cells from the AML-infiltrated skin from an M6 patient were infected with a lentivirus carrying OCT4, SOX2, KLF4 and C-MYC to induce iPSCs. The characteristics of the iPSCs were confirmed by alkaline phosphatase (ALP) staining. The proliferation ability of iPSCs was detected with a CCK-8 assay. The expression of pluripotency markers was measured by immunostaining, and the expression of stem cell-related genes was detected by qRT-PCR; distortion during the induction process was detected by karyotype analysis; the differentiation potential of iPSCs was determined by embryoid body-formation and teratoma-formation assays. ALP staining confirmed that these cells exhibited positive staining and had the characteristics of iPSCs.
  
  
  RESULTS
  The CCK-8 assay showed that the iPSCs had the ability to proliferate. Immunostaining demonstrated that iPSC clones showed positive expression of NANOG, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. qRT-PCR results revealed that the mRNA expression of Nanog, Lin28, Cripto, FOX3, DNMT3b, DPPA2, and DPPA4 significantly increased in iPSCs. Karyotype analysis found no chromosome aberration in the iPSCs. The results of the embryoid body-formation and teratoma-formation assays indicated that the iPSCs had the potential to differentiate into all three germ layers.
  
  
  CONCLUSIONS
  Our study provided evidence that an iPSC line derived from AML cells was successfully established.
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Liang-Fang
authors.initials: LF
authors.lastname: Zhu
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Qiu-Ru
authors.initials: QR
authors.lastname: Chen
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Shao-Zhen
authors.initials: SZ
authors.lastname: Chen
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Luo
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Ren
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Yuan
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Xue-Qiong
authors.initials: XQ
authors.lastname: Wu
authors.affiliation: Department of Hematology, Affiliated Nanping First Hospital of Fujian Medical University, Nanping, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zeng
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Chen
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Ying-Yu
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Lin
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Zheng-Jun
authors.initials: ZJ
authors.lastname: Wu
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Zhi-Zhe
authors.initials: ZZ
authors.lastname: Chen
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Jian-Da
authors.initials: JD
authors.lastname: Hu
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
mesh_terms: D000328:Adult; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D000090062:Kruppel-Like Factor 4; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D009363:Neoplasm Proteins; D014157:Transcription Factors
keywords: Acute myelogenous leukemia; C-MYC; Cell pluripotent factor; Induced pluripotent stem cell; KLF4; OCT4; Reprogramming; SOX2