title: Differential expression of ten-eleven translocation genes in endometrial cancers.
abstract: Ten-eleven translocation proteins are α-ketoglutarate-dependent dioxygenases involved in the conversion of 5-methylcytosines (5-mC) to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine, and 5-carboxylcytosine that play a significant role in DNA demethylation. Deregulation of TET genes expression and changes in the level of 5-hmC are thought to be associated with the onset and progression of several types of cancer, but there are no such data related to endometrial cancer. The aim of the work was to investigate the messenger RNA expression levels of TET1, TET2, and TET3 in relation to clinicopathological characteristics of endometrial cancer as well as the correlation between expression of TET genes and the level of 5-hmC/5-mC. The prognostic significance of TETs expression for overall survival was established. We found that TET1 and TET2 messenger RNA expression was lower and TET3 was higher in cancers compared to normal tissues. Positive correlation between 5-hmC and the relative expression of TET1 and TET2 was found, but no correlation was observed in the case of TET3. Decreased expression of TET1 and TET2 was significantly associated with increased lymph node metastasis and International Federation of Gynecology and Obstetrics stage. Kaplan-Meier analysis indicated that low TET1 expression predicted poor overall survival (p = 0.038). Multivariate analysis identified the TET1 expression in endometrial cancer as an independent prognostic factor. Our results suggest that decreased expression of TET1 correlates with tumor progression and may serve as a potential prognostic biomarker in endometrial cancer.
authors.affiliation: 1 Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ciesielski
authors.affiliation: 1 Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Jóźwiak
authors.affiliation: 2 Clinical Division of Gynecological Oncology, Medical University of Lodz, Łódź, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wójcik-Krowiranda
authors.affiliation: 1 Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Forma
authors.affiliation: 2 Clinical Division of Gynecological Oncology, Medical University of Lodz, Łódź, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Cwonda
authors.affiliation: 2 Clinical Division of Gynecological Oncology, Medical University of Lodz, Łódź, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Szczepaniec
authors.affiliation: 2 Clinical Division of Gynecological Oncology, Medical University of Lodz, Łódź, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bieńkiewicz
authors.affiliation: 1 Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bryś
authors.affiliation: 1 Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Krześlak
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives / Q000378:metabolism; D000368:Aged; D003596:Cytosine / Q000031:analogs & derivatives / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D049308:Dioxygenases / Q000096:biosynthesis* / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C560974:5-carboxylcytosine; C560973:5-formylcytosine; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D006899:Mixed Function Oxygenases; C462539:TET1 protein, human; C578792:TET3 protein, human; D049308:Dioxygenases; C541081:TET2 protein, human
keywords: 5-hydroxymethylcytosine; Ten-eleven translocation; endometrial cancer; prognostic factor