title: High cell density cultivation of six fungal strains efficient in azo dye bioremediation.
abstract: This work aims at optimizing the high cell density fungal cultivation for producing large quantities of fungal biomass to be used in azo dye residues bioremediation. In our previous studies the efficacy of using certain fungal strains to decolorize a range of commercial textile dyes of different structures (azo, disazo) were investigated. Several promising fungal strains belonging toAspergillus tubigenesis,Aspergillus niger,Aspergillus terreus,andAspergillus fumigatesdemonstrated high capacity in decolorizing various azo dyes. This study focuses on the high cell density cultivation of the fungal strains identified as potential bioremediation agents. The study includes the optimization of all parameters involved in bioprocess development for high cell density cultivation of six promising fungal strains. The growth of the fungal strains was tested on the sucrose medium in 7 l-fermenter. The growth of these fungal strains having the capacity to accumulate large quantities of biomass was also tested in medium containing molasses as a cheap substrate. The residual molasses, biomass dry weight and protein content of the six fungal strains showed that the strains 20 and 2 were marked by the highest protein content. In this study a comparative analysis between the results of dry weight, residual molasses and protein content of geowth of the strains 20, 5 and 2 under uncontrolled and controlled pH of media in batch fermentation was studied to follow the accumulation of biomass and protein production in the growth media. The results indicate that the dry weight accumulated by strains No. 20, 5 and 2 grown on molasses was better than those of strains grown on sucrose. Fungal strain No. 5 had the highest biomass dry weight accumulation. The study shows that the molasses as cheaper sugar sources were better than sucrose for growing fungal biomass.
authors.affiliation: Agricultural Microbiology Department, National Research Centre (NRC), Dokki, Cairo, Egypt; Biotechnology & Genetic Engineering Pilot Plant Unit, National Research Centre (NRC), Dokki, Cairo, Egypt.
authors.forename: Wafaa M
authors.initials: WM
authors.lastname: Abd El-Rahim
authors.affiliation: Chemical Engineering & Pilot Plant Department, National Research Centre (NRC), Dokki, Cairo, Egypt.
authors.forename: Enas M
authors.initials: EM
authors.lastname: Mostafa
authors.affiliation: Agricultural Microbiology Department, National Research Centre (NRC), Dokki, Cairo, Egypt.
authors.forename: Hassan
authors.initials: H
authors.lastname: Moawad
keywords: Azo textile dye; Controlled pH; Fungal cultivation; Molasses