title: Hydrogen Sulphide Production in Healthy and Ulcerated Gastric Mucosa of Rats.
abstract: Hydrogen sulphide (H₂S) is produced endogenously via two enzymes dependent on pyridoxal phosphate (PLP): cystathionine beta-synthase (CBS, EC 4.2.1.22), cystathionase γ-liase (CTH, EC 4.4.1.1), and a third, 3-mercaptopyruvate sulfurtransferase (MPST, EC 2.8.1.2). H₂S strengthens the defence mechanisms of the gastric mucosal barrier, and plays an important role in gastroprotection, including the increased resistance to damage caused by various irritants and non-steroidal anti-inflammatory drugs. The study was conducted to determine the role of H₂S in ulcerated gastric mucosa of rats caused by immobilization in cold water (WRS). The activity and expression of γ-cystathionase, cystathionine β-synthase, 3-mercaptopyruvate sulfurtransferase, and rhodanese was compared with healthy mucosa, together with H₂S generation, and cysteine, glutathione, and cystathionine levels. The results showed that the defence mechanism against stress is associated with stimulation of the production of H₂S in the tissue and confirmed the observed advantageous effect of H₂S on healing of gastric ulcers. In case of animals pretreated with exogenous sources of H₂S and NaHS, and some changes observed in the ulcerated gastric mucosa tend to return to values found in the healthy tissue, a finding that is in accordance with the previously determined gastroprotective properties of H₂S. The results presented in this paper point to the possible role of rhodanese in H₂S production in the gastric mucosa of rats, together with the earlier mentioned three enzymes, which are all active in this tissue.
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Medical College, Krakow, 7 Kopernika St., 31-034 Cracow, Poland. mbbronow@cyf-kr.edu.pl.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Bronowicka-Adamska
authors.affiliation: Chair of Medical Biochemistry, Jagiellonian University Medical College, Krakow, 7 Kopernika St., 31-034 Cracow, Poland. mbwrobel@cyf-kr.edu.pl.
authors.forename: Maria
authors.initials: M
authors.lastname: Wróbel
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 31-530 Krakow, Poland. magierowskim@yahoo.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Magierowski
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 31-530 Krakow, Poland. k.jasnos@interia.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Magierowska
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 31-530 Krakow, Poland. skwiecien@cm-uj.krakow.pl.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Kwiecień
authors.affiliation: Department of Physiology, Jagiellonian University Medical College, 31-530 Krakow, Poland. mpbrzozo@cyf-kr.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Brzozowski
mesh_terms: D000818:Animals; D003541:Cystathionine beta-Synthase / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006862:Hydrogen Sulfide / Q000008:administration & dosage* / Q000378:metabolism; D008297:Male; D051381:Rats; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism
chemical_list: D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; C021550:3-mercaptopyruvate sulphurtransferase; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide
keywords: 3-mercaptopyruvate sulfurtransferase; NaHS; cystathionine-β-synthase; gastric mucosa; hydrogen sulphide; rhodanese; γ-cystathionase