title: Characterization of suspended bacteria from processing units in an advanced drinking water treatment plant of China.
abstract: For the drinking water treatment plant (DWTP), the organic pollutant removal was the primary focus, while the suspended bacterial was always neglected. In this study, the suspended bacteria from each processing unit in a DWTP employing an ozone-biological activated carbon process was mainly characterized by using heterotrophic plate counts (HPCs), a flow cytometer, and 454-pyrosequencing methods. The results showed that an adverse changing tendency of HPC and total cell counts was observed in the sand filtration tank (SFT), where the cultivability of suspended bacteria increased to 34%. However, the cultivability level of other units stayed below 3% except for ozone contact tank (OCT, 13.5%) and activated carbon filtration tank (ACFT, 34.39%). It meant that filtration processes promoted the increase in cultivability of suspended bacteria remarkably, which indicated biodegrading capability. In the unit of OCT, microbial diversity indexes declined drastically, and the dominant bacteria were affiliated to Proteobacteria phylum (99.9%) and Betaproteobacteria class (86.3%), which were also the dominant bacteria in the effluent of other units. Besides, the primary genus was Limnohabitans in the effluents of SFT (17.4%) as well as ACFT (25.6%), which was inferred to be the crucial contributors for the biodegradable function in the filtration units. Overall, this paper provided an overview of community composition of each processing units in a DWTP as well as reference for better developing microbial function for drinking water treatment in the future.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai, 200092, P. R. China.|College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai, 200092, P. R. China. 123lwyktz@tongji.edu.cn.|Key Laboratory of Yangtze Aquatic Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China. 123lwyktz@tongji.edu.cn.
authors.forename: Weiying
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.|Key Laboratory of Yangtze Aquatic Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.
authors.forename: Junpeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai, 200092, P. R. China.|College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.
authors.forename: Wanqi
authors.initials: W
authors.lastname: Qi
authors.affiliation: College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.|Key Laboratory of Yangtze Aquatic Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai, 200092, P. R. China.|College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.|Key Laboratory of Yangtze Aquatic Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P. R. China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Shi
mesh_terms: D001419:Bacteria / Q000145:classification; D002606:Charcoal; D002681:China; D060766:Drinking Water / Q000382:microbiology; D005374:Filtration; D018508:Water Purification
chemical_list: D060766:Drinking Water; D002606:Charcoal
keywords: 454-Pyrosequencing; Biodegrading capability; Drinking water treatment plant; Flow cytometer; Suspended bacteria