title: Surface-imprinted magnetic nanoparticles for the selective enrichment and fast separation of fluoroquinolones in human serum.
abstract: Surface enrofloxacin-imprinted magnetic nanoparticles were prepared for the selective recognition and fast separation of fluoroquinolones in human serum by surface-initiated reversible addition fragmentation chain transfer polymerization. The surface morphology and imprinted behavior were investigated and optimized. The living/controlled nature of reversible addition-fragmentation chain transfer polymerization reaction allowed the successful construction of well-defined imprinted polymer layer outside the Fe3O4core. Such molecularly imprinted polymers exhibited superparamagnetic properties and specific recognition toward fluoroquinolones. Combined with reversed-phase high-performance liquid chromatography, the prepared molecularly imprinted polymers were used for the selective enrichment and analysis of fluoroquinolones in human serum samples. The recoveries of four fluoroquinolones were 86.8-95.3% with relative standard deviations of 2.0-6.8% (n = 3). Such magnetic molecularly imprinted polymers have great prospects in the separation and enrichment of trace analysts in complex biological samples.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Su
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
mesh_terms: D024841:Fluoroquinolones / Q000097:blood*; D006801:Humans; D008280:Magnetics; D058185:Magnetite Nanoparticles; D054802:Molecular Imprinting; D011108:Polymers
chemical_list: D024841:Fluoroquinolones; D058185:Magnetite Nanoparticles; D011108:Polymers
keywords: fluoroquinolones; human serum; molecularly imprinted polymers; polymerization; superparamagnetism