title: The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton.
abstract: Target of rapamycin (TOR) acts as an important regulator of cell growth, development and stress responses in most examined diploid eukaryotes. However, little is known about TOR in tetraploid species such as cotton. Here, we show that TORC1-S6K-RPS6, the major signaling components, are conserved and further expanded in cotton genome. Though the cotton seedlings are insensitive to rapamycin, AZD8055, the second-generation inhibitor of TOR, can significantly suppress the growth in cotton. Global transcriptome analysis revealed that genes associated with jasmonic acid (JA) biosynthesis and transduction were significantly altered in AZD8055 treated cotton seedlings, suggesting the potential crosstalk between TOR and JA signaling. Pharmacological and genetic approaches have been employed to get further insights into the molecular mechanism of the crosstalk between TOR and JA. Combination of AZD8055 with methyl jasmonate can synergistically inhibit cotton growth, and additionally JA levels were significantly increased when cotton seedlings were subjected to AZD8055. JA biosynthetic and signaling mutants including jar1, coi1-2 and myc2-2 displayed TOR inhibitor-resistant phenotypes, whereas COI1 overexpression transgenic lines and jaz10 exhibited sensitivity to AZD8055. Consistently, cotton JAZ can partially rescue TOR-suppressed phenotypes in Arabidopsis. These evidences revealed that the crosstalk between TOR and JA pathway operates in cotton and Arabidopsis.
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Song
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Chongqing University, Chongqing, China.
authors.forename: Linxuan
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Life Sciences, Chongqing University, Chongqing, China.
authors.forename: Fangjie
authors.initials: F
authors.lastname: Xiong
authors.affiliation: School of Life Sciences, Chongqing University, Chongqing, China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Zhuo
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
authors.forename: Zuoren
authors.initials: Z
authors.lastname: Yang
authors.affiliation: National Research Council of Canada, Saskatoon, Canada.
authors.forename: Raju
authors.initials: R
authors.lastname: Datla
authors.affiliation: School of Life Sciences, Chongqing University, Chongqing, China.
authors.forename: Maozhi
authors.initials: M
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
authors.forename: Fuguang
authors.initials: F
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009025:Morpholines / Q000494:pharmacology; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction; D020123:Sirolimus / Q000378:metabolism*
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D009025:Morpholines; D054883:Oxylipins; C011006:jasmonic acid; C072239:methyl jasmonate; C546624:(5-(2,4-bis((3S)-3-methylmorpholin-4-yl)pyrido(2,3-d)pyrimidin-7-yl)-2-methoxyphenyl)methanol; D019869:Phosphatidylinositol 3-Kinases; C512171:TOR protein, Arabidopsis; D020123:Sirolimus