title: Clinical, cellular, and bioinformatic analyses reveal involvement of WRAP53 overexpression in carcinogenesis of lung adenocarcinoma.
abstract: Lung cancer, of which non-small cell lung cancer accounts for 80%, remains a leading cause of cancer-related mortality and morbidity worldwide. Our study revealed that the expression of WD repeat containing antisense to P53 (WRAP53) is higher in lung-adenocarcinoma specimens than in specimens from adjacent non-tumor tissues. The prevalence of WRAP53 overexpression was significantly higher in patients with tumor larger than 3.0 cm than in patients with tumor smaller than 3.0 cm. The depletion of WRAP53 inhibits the proliferation of lung-adenocarcinoma A549 and SPC-A-1 cells via G1/S cell-cycle arrest. Several proteins interacting with WRAP53 were identified through co-immunoprecipitation and liquid chromatography/mass spectrometry. These key proteins indicated previously undiscovered functions of WRAP53. These observations strongly suggested that WRAP53 should be considered a promising target in the prevention or treatment of lung adenocarcinoma.
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Xiao-Shuai
authors.initials: XS
authors.lastname: Yuan
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Long-Xiang
authors.initials: LX
authors.lastname: Cao
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Ye-Ji
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Fei-Chao
authors.initials: FC
authors.lastname: Bao
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Zhi-Tian
authors.initials: ZT
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Cao
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Lv
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D019295:Computational Biology; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D016196:S Phase / Q000502:physiology; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018832:Molecular Chaperones; D019098:Telomerase; C535133:WRAP53 protein, human
keywords: A549 cells; SPC-A-1 cells; WRAP53; cell-cycle arrest; clinical characteristics; double-thymidine blocking; liquid chromatography/mass spectrometry; lung adenocarcinoma