title: Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics.
abstract: There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid-liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), 28040, Madrid, Spain. silvia@cib.csic.es monterroso@cib.csic.es grivas@cib.csic.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Sobrinos-Sanguino
authors.forename: Silvia
authors.initials: S
authors.lastname: Zorrilla
authors.forename: Christine D
authors.initials: CD
authors.lastname: Keating
authors.forename: Begoña
authors.initials: B
authors.lastname: Monterroso
authors.forename: Germán
authors.initials: G
authors.lastname: Rivas
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D008055:Lipids