title: Highly Time-Resolved Metabolic Reprogramming toward Differential Levels of Phosphate inChlamydomonas reinhardtii.
abstract: Understanding phosphorus metabolism in photosynthetic organisms is important as it is closely associated with enhanced crop productivity and pollution management for natural ecosystems (e.g., algal blooming). Accordingly, we exploited highly time-resolved metabolic responses to different levels of phosphate deprivation inChlamydomonas reinhardtii, a photosynthetic model organism. We conducted non-targeted primary metabolite profiling using gas-chromatography time-of-flight mass spectrometric analysis. Primarily, we systematically identified main contributors to degree-wise responses corresponding to the levels of phosphate deprivation. Additionally, we systematically characterized the metabolite sets specific to different phosphate conditions and their interactions with culture time. Among them were various types of fatty acids that were most dynamically modulated by the phosphate availability and culture time in addition to phosphorylated compounds.
authors.affiliation: Depatment of Bio and Fermentation Convergence Technology, BK21 PLUS Program, Kookmin University, Seoul 02707, Republic of Korea.
authors.forename: Cheol-Ho
authors.initials: CH
authors.lastname: Jang
authors.affiliation: Depatment of Bio and Fermentation Convergence Technology, BK21 PLUS Program, Kookmin University, Seoul 02707, Republic of Korea.
authors.forename: Gayeon
authors.initials: G
authors.lastname: Lee
authors.affiliation: Depatment of Bio and Fermentation Convergence Technology, BK21 PLUS Program, Kookmin University, Seoul 02707, Republic of Korea.
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Park
authors.affiliation: Depatment of Biotechnology, Graduate School, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Depatment of Bio and Fermentation Convergence Technology, BK21 PLUS Program, Kookmin University, Seoul 02707, Republic of Korea.
authors.forename: Do Yup
authors.initials: DY
authors.lastname: Lee
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D055442:Metabolome; D055432:Metabolomics; D010641:Phenotype; D010710:Phosphates / Q000378:metabolism* / Q000494:pharmacology; D010788:Photosynthesis; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D010710:Phosphates
keywords: Chlamydomonas reinhardtii; GC-TOF MS; dose responsiveness; phosphorus; temporal resolution