title: Effects of four kinds of electromagnetic fields (EMF) with different frequency spectrum bands on ovariectomized osteoporosis in mice.
abstract: Electromagnetic fields (EMF) was considered as a non-invasive modality for treatment of osteoporosis while the effects were diverse with EMF parameters in time domain. In present study, we extended analysis of EMF characteristics from time domain to frequency domain, aiming to investigate effects of four kinds of EMF (LP (1-100 Hz), BP (100-3,000 Hz), HP (3,000-50,000 Hz) and AP (1-50,000 Hz)) on ovariectomized (OVX) osteoporosis (OP) in mice. Forty-eight 3-month-old female BALB/c mice were equally assigned to Sham, OVX, OVX + LP, OVX + BP, OVX + HP and OVX + AP groups (n = 8). After 8-week exposure (3 h/day), LP and BP significantly increased serum bone formation markers and osteogenesis-related gene expressions compared with OVX. Bedsides, LP and BP also slightly increased bone resorption activity compared with OVX, evidenced by increased RANKL/OPG ratio. HP sharply decreased serum bone formation and resporption markers and osteogenesis and osteoclastogenesis related gene expressions compared with OVX. AP had accumulative effects of LP, BP and HP, which significantly increased bone formation and decreased bone resporption activity compared with OVX. As a result, LP, BP and HP exposure did not later deterioration of bone mass, microarchitecture and mechanical strength in OVX mice with OP. However, AP stimulation attenuated OVX-induced bone loss.
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lei
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Feijiang
authors.initials: F
authors.lastname: Li
authors.affiliation: Institute of Orthopaedics, Xijing hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Zhuowen
authors.initials: Z
authors.lastname: Liang
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Tang
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Kangning
authors.initials: K
authors.lastname: Xie
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Dong
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shan
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Nursing, Fourth Military Medical University, 17 West Changle Road, Xi'an, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Xu
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China. erpingluo@hotmail.com.
authors.forename: Erping
authors.initials: E
authors.lastname: Luo
authors.affiliation: School of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, China. guanghaoshen@outlook.com.
authors.forename: Guanghao
authors.initials: G
authors.lastname: Shen
mesh_terms: D000818:Animals; D015415:Biomarkers; D001835:Body Weight; D015519:Bone Density; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D004195:Disease Models, Animal; D004574:Electromagnetic Fields; D005260:Female; D006801:Humans; D055909:Magnetic Field Therapy / Q000379:methods; D051379:Mice; D010024:Osteoporosis / Q000175:diagnosis* / Q000209:etiology* / Q000378:metabolism / Q000628:therapy; D015663:Osteoporosis, Postmenopausal / Q000097:blood / Q000175:diagnosis / Q000378:metabolism / Q000628:therapy; D010052:Ovariectomy / Q000009:adverse effects; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers