title: Expression of kit ligand and insulin-like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep.
abstract: Expression of Kit ligand (KL) and insulin-like growth factor binding protein (IGFBP3) genes was studied at different in vivo and corresponding in vitro stages of development of the ovarian follicles in sheep. The expression of both KL and IGFBP3 was significantly higher in the primordial follicles relative to any other stage of development. Compared to the other stages, the KL expression in the cumulus cells from in vivo grown large antral follicles and that of IGFBP3 in COCs' isolated from large antral follicles matured in vitro for 24 hr were significantly higher. In the oocytes from in vivo grown ovarian follicles, the expression of KL was the same at all the stages of development. Insulin-like growth factor binding protein 3 expression was also the same in the oocytes at all the stages of the development except for a significantly lower expression in those from antral follicles. The expression of KL in the cumulus cells decreased significantly in the in vitro grown early antral follicles but did not change further as the development progressed. The expression of IGFBP3 in the cumulus cells from in vitro grown ovarian follicles appeared to increase as the development progressed although the increase was not significant between any two consecutive stages of development. In the oocytes in in vitro grown ovarian follicles, the expression levels of KL and IGFBP3 genes did not change with development. It is concluded that (i) KL and IGFBP3 genes follow specific patterns of expression during ovarian folliculogenesis and (ii) in vitro culture of preantral follicles compromises the development potential through alterations in the stage-specific patterns of expression of these and other developmentally important genes.
authors.affiliation: Department of Animal Reproduction and Gynecology, College of Veterinary Science, S.V. Veterinary University, Tirupati, India.
authors.forename: D
authors.identifier: http://orcid.org/0000-0001-5231-7032
authors.initials: D
authors.lastname: Srividya
authors.affiliation: Embryo Biotechnology Laboratory, Department of Physiology, College of Veterinary Science, S.V.Veterinary University, Tirupati, India.
authors.forename: V
authors.initials: V
authors.lastname: Praveen Chakravarthi
authors.affiliation: Embryo Biotechnology Laboratory, Department of Physiology, College of Veterinary Science, S.V.Veterinary University, Tirupati, India.
authors.forename: Ssr
authors.initials: S
authors.lastname: Kona
authors.affiliation: Embryo Biotechnology Laboratory, Department of Physiology, College of Veterinary Science, S.V.Veterinary University, Tirupati, India.
authors.forename: Avn
authors.initials: A
authors.lastname: Siva Kumar
authors.affiliation: Department of Animal Reproduction and Gynecology, College of Veterinary Science, S.V. Veterinary University, Tirupati, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Brahmaiah
authors.affiliation: Embryo Biotechnology Laboratory, Department of Physiology, College of Veterinary Science, S.V.Veterinary University, Tirupati, India.
authors.forename: V H
authors.identifier: http://orcid.org/0000-0001-8623-0138
authors.initials: VH
authors.lastname: Rao
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000254:growth & development* / Q000378:metabolism; D012756:Sheep; D019089:Stem Cell Factor / Q000235:genetics* / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000662:veterinary
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D019089:Stem Cell Factor