title: MicroBubble activated acoustic cell sorting.
abstract: Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave. In acoustophoresis, cells being positive acoustic contrast particles migrate to pressure nodes. On the contrary, air-filled polymer-shelled microbubbles being strong negative acoustic contrast particles migrate to pressure antinodes and can be used to selectively migrate target cells. As a proof of principle, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency. Moreover, 100% of the microbubble-cell conjugates migrated to the anti-node. Hence a better upstream affinity-capture has the potential to provide higher sorting efficiency. The BAACS technique expands the acoustic cell manipulation possibilities and offers cell-sorting solutions suited for applications at point of care.
authors.affiliation: Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
authors.forename: M A
authors.initials: MA
authors.lastname: Faridi
authors.affiliation: Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Ramachandraiah
authors.affiliation: Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.|Deptartment of Applied Physics, School of Engineering Science, KTH Royal Institute of Technology, Stockholm, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Iranmanesh
authors.affiliation: Department of Medical Engineering, School of Technology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Grishenkov
authors.affiliation: Deptartment of Applied Physics, School of Engineering Science, KTH Royal Institute of Technology, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Wiklund
authors.affiliation: Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. aman.russom@scilifelab.se.
authors.forename: A
authors.identifier: 0000-0002-0242-358X
authors.initials: A
authors.lastname: Russom
mesh_terms: D000162:Acoustics; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D056656:Lab-On-A-Chip Devices; D045423:Microbubbles
keywords: Acoustophoresis; Cell sorting; Contrast agent; Microbubble; Microfluidic separation