title: Quorum quenching properties of Actinobacteria isolated from Malaysian tropical soils.
abstract: In this study, a total of 147 soil actinobacterial strains were screened for their ability to inhibit response of Chromobacterium violaceum CV026 to short chain N-acyl homoserine lactone (AHL) which is a quorum sensing molecule. Of these, three actinobacterial strains showed positive for violacein inhibition. We further tested these strains for the inhibition of Pseudomonas aeruginosa PAO1 quorum sensing-regulated phenotypes, namely, swarming and pyocyanin production. The three strains were found to inhibit at least one of the quorum sensing-regulated phenotypes of PAO1. Phylogenetic analysis of the 16S rRNA gene sequences indicated that these strains belong to the genera Micromonospora, Rhodococcus and Streptomyces. This is the first report presenting quorum quenching activity by a species of the genus Micromonospora. Our data suggest that Actinobacteria may be a rich source of active compounds that can act against bacterial quorum sensing system.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Kavimalar
authors.initials: K
authors.lastname: Devaraj
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. gyatan@um.edu.my.
authors.forename: Geok Yuan Annie
authors.initials: GYA
authors.lastname: Tan
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.identifier: http://orcid.org/0000-0002-1883-1115
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D002861:Chromobacterium / Q000502:physiology; D008296:Malaysia; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D011710:Pyocyanine; D053038:Quorum Sensing; D012988:Soil Microbiology
chemical_list: C088386:homoserine lactone; D011710:Pyocyanine; D015107:4-Butyrolactone
keywords: Chromobacterium violaceum CV026; Micromonospora; N-Acyl homoserine lactones; Pseudomonas aeruginosa PAO1; Quorum quenching; Quorum sensing; Rhodococcus; Streptomyces