title: Two-Dimensional Gel Electrophoresis: A Reference Protocol.
abstract: Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s. Over this time, it has been used in the study of many diseases including cancer, diabetes, heart disease, and psychiatric disorders through the proteomic analysis of body fluids and tissues. This chapter presents a general protocol which can be applied in the study of biological samples such as blood serum or plasma and multiple tissues including the brain.
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), RuaMonteiroLobato, 25513083-862, Campinas, SP, Brazil.
authors.forename: Veronica M
authors.initials: VM
authors.lastname: Saia-Cereda
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), RuaMonteiroLobato, 25513083-862, Campinas, SP, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Aquino
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), RuaMonteiroLobato, 25513083-862, Campinas, SP, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), RuaMonteiroLobato, 25513083-862, Campinas, SP, Brazil. dmsouza@unicamp.br.|UNICAMP's Neurobiology Center, Campinas, Brazil. dmsouza@unicamp.br.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue
keywords: 2D-DIGE; 2DE; 2DE-MS; Proteomics; Two-dimensional gel electrophoresis