title: Comparative proteomics to reveal muscle-specific beef color stability of Holstein cattle during post-mortem storage.
abstract: Label-free strategy was applied to elucidate muscle-specific beef (M. longissimuss lumborum (LL) and M. psoas major (PM)) color stability of Holstein cattle during post-mortem storage at 4°C±1°C. LL showed greater (p<0.05) redness (a∗) value than PM at day 4 and 9 storage, while the proportion of metmyoglobin in PM exhibited a greater increase than in LL muscle. Furthermore, an overabundance of proteins with the functions of antioxidation, protection, and repair in LL were conducive to its color stability, whereas the overabundant proteins/subunits involved in tricarboxylic acid (TCA) cycle and mitochondrial electron transport chain (ETC) in PM indicated greater oxidative metabolism and degradation of ETC complexes, resulting in poor color stability. Bioinformatic analyses indicated that these proteins mainly participated in oxidation-reduction processes, TCA cycle, and ETC processes. All of these results provided a deeper understanding of muscle-specific beef color stability from the perspective of proteomics.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Tian
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jia
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China. Electronic address: dairuitong@hotmail.com.
authors.forename: Ruitong
authors.initials: R
authors.lastname: Dai
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Xingmin
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D008786:Metmyoglobin / Q000032:analysis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000032:analysis* / Q000378:metabolism; D060066:Protein Interaction Maps / Q000502:physiology; D040901:Proteomics / Q000379:methods*; D000069466:Red Meat / Q000032:analysis*
chemical_list: D000975:Antioxidants; D010860:Pigments, Biological; D008786:Metmyoglobin
keywords: Color stability; Label-free; M. longissimuss lumborum; M. psoas major; Proteomics