title: Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation.
abstract: OBJECTIVE
  This study aims to assess the effect of ozone gas on dentin exposed to Streptococcus mutans biofilm by evaluation of mineral content [log calcium-to-phosphorus (Ca/P)] using energy-dispersive X-ray (EDX) spectroscopy.
  
  
  METHODS
  Five human third molars were sectioned into four slices of dentin and distributed in four groups: I - control (no treatment); II - ozone therapy; III - biofilm development; IV - ozone therapy followed by biofilm development. Mineral content (log Ca/P) was evaluated by EDX. Data were analyzed by analysis of variance and Tukey's test (p < 0.05).
  
  
  RESULTS
  Results showed that the mineral content of control group (I) was similar to ozone group (II), and was statistically higher than biofilm (III) and ozone + biofilm (IV). The lowest log Ca/P was determined in biofilm group (III).
  
  
  CONCLUSIONS
  It can be concluded that ozone gas did not grant preventive effects of demineralization by S. mutans biofilm on dentin surface.
  
  
  CONCLUSIONS
  Ozone gas therapy may be an alternative noninvasive treatment aiming to reduce the levels of caries-associated microorganisms. This therapy may, thereby, be an alternative and/or complementary treatment strategy in preventive dentistry.
authors.affiliation: Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás Brazil.
authors.forename: Rafaella M
authors.initials: RM
authors.lastname: Chaves
authors.affiliation: Department of Stomatological Sciences, School of Dentistry Federal University of Goiás, Goiânia, Goiás, Brazil, e-mail: estrela3@terra.com.br.
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
authors.affiliation: Restorative Dentistry Department, Brazilian Dental Association Goiânia, Goiás, Brazil.
authors.forename: Paula C
authors.initials: PC
authors.lastname: Cardoso
authors.affiliation: Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás Brazil.
authors.forename: Terezinha
authors.initials: T
authors.lastname: de Je Barata
authors.affiliation: Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás Brazil.
authors.forename: João B
authors.initials: JB
authors.lastname: de Souza
authors.affiliation: Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás Brazil.
authors.forename: Érica M
authors.initials: ÉM
authors.lastname: de Torres
authors.affiliation: Department of Stomatological Sciences, School of Dentistry Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Cyntia Ra
authors.initials: CR
authors.lastname: Estrela
authors.affiliation: Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás Brazil.
authors.forename: Ana Pr
authors.initials: AP
authors.lastname: Magalhães
authors.affiliation: Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás Brazil.
authors.forename: Lawrence G
authors.initials: LG
authors.lastname: Lopes
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002118:Calcium / Q000032:analysis*; D003804:Dentin / Q000737:chemistry / Q000187:drug effects* / Q000382:microbiology; D006801:Humans; D010126:Ozone / Q000009:adverse effects*; D010758:Phosphorus / Q000032:analysis*; D013052:Spectrometry, X-Ray Emission; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D010758:Phosphorus; D010126:Ozone; D002118:Calcium
keywords: Biofilm; Dental caries; Dentin; Laboratory research Ozone.