title: Glu-Phe from onion (Allium Cepa L.) attenuates lipogenesis in hepatocytes.
abstract: A Glu-Phe (EF) was isolated from onion (Allium cepa L. cv. Sunpower). The chemical structure of EF was determined by nuclear magnetic resonance and electrospray ionization-mass (ESI-MS) spectroscopy. We showed that EF reduced lipid accumulation in mouse hepatocytes by inhibiting the expression of sterol regulatory element-binding protein-1c (SREBP-1c) and its lipogenic target genes. We also found that AMP-activated protein kinase (AMPK) was required for the inhibitory effect of EF on lipid accumulation in mouse hepatocytes. Furthermore, EF was qualified in nine onion cultivars by selective multiple reaction-monitoring detection of liquid chromatography-ESI-MS. These results suggest that EF could contribute to the beneficial effect of onion supplement in maintaining hepatic lipid homeostasis.
authors.affiliation: a Department of Food Science and Technology and Functional Food Research Center, BK21 Plus Program , Chonnam National University , Gwangju , South Korea.
authors.forename: Yu Geon
authors.initials: YG
authors.lastname: Lee
authors.affiliation: b Bioenergy Crop Research Center , National Institute of Crop Science, Rural Development Administration , Muan , South Korea.
authors.forename: Jeong-Yong
authors.initials: JY
authors.lastname: Cho
authors.affiliation: b Bioenergy Crop Research Center , National Institute of Crop Science, Rural Development Administration , Muan , South Korea.
authors.forename: Eom Ji
authors.initials: EJ
authors.lastname: Hwang
authors.affiliation: c Department of Animal Science , Chonnam National University , Gwangju , South Korea.
authors.forename: Tae-Il
authors.initials: TI
authors.lastname: Jeon
authors.affiliation: a Department of Food Science and Technology and Functional Food Research Center, BK21 Plus Program , Chonnam National University , Gwangju , South Korea.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Moon
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004151:Dipeptides / Q000302:isolation & purification / Q000494:pharmacology*; D054890:Fatty Acid Synthase, Type I / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D050155:Lipogenesis / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D019697:Onions / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D015398:Signal Transduction; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004151:Dipeptides; D000960:Hypolipidemic Agents; D010936:Plant Extracts; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; C020621:gamma-glutamylphenylalanine; C490333:Scd1 protein, mouse; D013230:Stearoyl-CoA Desaturase; D054890:Fatty Acid Synthase, Type I; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase
keywords: SREBP-1c; dipeptide; hepatocytes; lipogenesis; onion