title: Cold Stress and Nitrogen Deficiency Affected Protein Expression of PsychrotrophicDyadobacter psychrophilusB2 andPseudomonas jesseniiMP1.
abstract: Nitrogen (N) deficiency and low temperature conditions are the prominent facet of Western Himalayan agro-ecosystems. A slight change in the environment alters the protein expression of the microorganisms. Therefore, proteomes of the two psychrotrophsDyadobacter psychrophilusB2 andPseudomonas jesseniiMP1 were analyzed using two dimensional electrophoresis and MALDI-TOF-MS, to determine the physiological response of altitudinally different but indigenous microorganisms in response to cold stress under N depleting conditions. Functional assessment of 150 differentially expressed proteins from both the psychrotrophs revealed several mechanisms might be involved in cold stress adaptation, protein synthesis/modifications, energy metabolism, cell growth/maintenance, etc. In both the proteomes, abundance of the proteins related to energy production and stress were significantly increased while, proteins related to biosynthesis and energy consuming processes decreased.ATP synthase subunit alpha, beta, ATP-dependent Clp protease, Enolase, groL HtpGandN(2)-fixation sustaining protein CowNproteins were found to be expressed in both B2 and MP1, similarly to previously studied diazotrophs under low temperature N2fixing conditions and therefore, can be considered as a biomarker for monitoring the nitrogen fixation in cold niches. Nevertheless, in both the diazotrophs, a good fraction of the proteins were related to hypothetical proteins which are still uncharacterized, thereby, suggesting the need for in-depth studies on cold adapted diazotrophs and their adaptive mechanisms.
authors.affiliation: Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, India.
authors.forename: Deep C
authors.initials: DC
authors.lastname: Suyal
authors.affiliation: Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune University Campus Pune, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune University Campus Pune, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Shouche
authors.affiliation: Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, India.
authors.forename: Reeta
authors.initials: R
authors.lastname: Goel
keywords: 2-D gel electrophoresis; Western Indian Himalaya; cold diazotrophy; differential proteomics; nitrogen fixation; psychrotrophs