title: Pupil segmentation adaptive optics for invivo mouse retinal fluorescence imaging.
abstract: Adaptive Optics (AO) for scanning laser ophthalmoscopy enables high-resolution retinal imaging that can be used for preclinical research of diseases causing vision loss. Pupil Segmentation (PS) is an approach to wavefront-sensorless AO that acquires images within subregions across the imaging pupil to measure the wavefront slopes at the corresponding locations of the beam. We present PS-AO as an approach to correct ocular aberrations in ∼7  s, implemented to minimize respiratory motion from an anesthetized mouse. We demonstrated an improvement in resolution and an image intensity increase of ∼25% across all results using PS-AO for in vivo fluorescence retinal imaging in mice using a MEMS-based segmented deformable mirror.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Wahl
authors.forename: Christine
authors.initials: C
authors.lastname: Huang
authors.forename: Stefano
authors.initials: S
authors.lastname: Bonora
authors.forename: Yifan
authors.initials: Y
authors.lastname: Jian
authors.forename: Marinko V
authors.initials: MV
authors.lastname: Sarunic