title: The differential plasma proteome of obese and overweight individuals undergoing a nutritional weight loss and maintenance intervention.
abstract: The nutritional intervention program "DiOGenes" focuses on how obesity can be prevented and treated from a dietary perspective. We generated differential plasma proteome profiles in the DiOGenes cohort to identify proteins associated with weight loss and maintenance and explore their relation to body mass index, fat mass, insulin resistance, and sensitivity.
  
  
  
  Relative protein quantification was obtained at baseline and after combined weight loss/maintenance phases using isobaric tagging and MS/MS. A Welch t-test determined proteins differentially present after intervention. Protein relationships with clinical variables were explored using univariate linear models, considering collection center, gender and age as confounding factors.
  
  
  
  Four hundred and seventy three subjects were measured at baseline and end of the intervention; 39 proteins were longitudinally differential. Proteins with largest changes were sex hormone-binding globulin, adiponectin, C-reactive protein, calprotectin, serum amyloid A, and proteoglycan 4 (PRG4), whose association with obesity and weight loss is known. We identified new putative biomarkers for weight loss/maintenance. Correlation between PRG4 and proline-rich acidic protein 1 variation and Matsuda insulin sensitivity increment was showed.
  
  
  
  MS-based proteomic analysis of a large cohort of non-diabetic overweight and obese individuals concomitantly identified known and novel proteins associated with weight loss and maintenance.
authors.affiliation: Systems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, Lausanne, Switzerland.|Signal and Information Processing for Sensing Systems, Institute for Bioengineering of Catalonia, Barcelona, Spain.
authors.forename: Sergio
authors.identifier: 0000-0002-8994-1549
authors.initials: S
authors.lastname: Oller Moreno
authors.affiliation: Systems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: Ornella
authors.initials: O
authors.lastname: Cominetti
authors.affiliation: Systems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: Antonio
authors.initials: A
authors.lastname: Núñez Galindo
authors.affiliation: Nutrition and Metabolic Health, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: Irina
authors.initials: I
authors.lastname: Irincheeva
authors.affiliation: Systems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: John
authors.initials: J
authors.lastname: Corthésy
authors.affiliation: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Arne
authors.initials: A
authors.lastname: Astrup
authors.affiliation: Department of Human Biology, NUTRIM, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
authors.forename: Wim H M
authors.initials: WHM
authors.lastname: Saris
authors.affiliation: Nutrition and Metabolic Health, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hager
authors.affiliation: Systems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Kussmann
authors.affiliation: Systems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors.forename: Loïc
authors.identifier: 0000-0002-8499-270X
authors.initials: L
authors.lastname: Dayon
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000235:genetics* / Q000378:metabolism; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D004038:Diet, Reducing / Q000379:methods; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D039841:Leukocyte L1 Antigen Complex / Q000097:blood / Q000235:genetics; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000178:diet therapy* / Q000235:genetics / Q000473:pathology; D011257:Pregnancy Proteins / Q000097:blood / Q000235:genetics; D011509:Proteoglycans / Q000097:blood / Q000235:genetics; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000235:genetics / Q000378:metabolism; D012738:Sex Hormone-Binding Globulin / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D015431:Weight Loss / Q000502:physiology; D061217:Weight Reduction Programs / Q000379:methods*; D000099158:Proteoglycan 4
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D001798:Blood Proteins; D002097:C-Reactive Protein; D039841:Leukocyte L1 Antigen Complex; D011257:Pregnancy Proteins; D011509:Proteoglycans; D020543:Proteome; D000685:Serum Amyloid A Protein; D012738:Sex Hormone-Binding Globulin; C499918:ADIPOQ protein, human; C419197:PRG4 protein, human; D000099158:Proteoglycan 4; C581328:proline-rich acidic protein 1, human
keywords: Biomarker; Diabetes; Large-scale study; Mass spectrometry; Obesity