title: Construction of Methanol-SensingEscherichia coliby the Introduction of aParacoccus denitrificansMxaY-Based Chimeric Two-Component System.
abstract: Escherichia coliwas engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing theParacoccus denitrificansMxaY with theE. coliEnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription ofompCand the fluorescence at 0.01% of methanol, respectively. These results suggested thatE. coliwas successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.
authors.affiliation: Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
authors.forename: Irisappan
authors.initials: I
authors.lastname: Ganesh
authors.affiliation: Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
authors.forename: Selvamani
authors.initials: S
authors.lastname: Vidhya
authors.affiliation: Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.|Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44610, Republic of Korea.|Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
authors.forename: Gyeong Tae
authors.initials: GT
authors.lastname: Eom
authors.affiliation: Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea.
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics; D000432:Methanol / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000235:genetics*; D010766:Phosphorylation; D018272:Porins / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
chemical_list: D029968:Escherichia coli Proteins; C113724:OmpC protein; D018272:Porins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D000432:Methanol
keywords: Chimeric two-component system; Escherichia coli; green fluorescent protein; methanol