title: An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 by using chitosan, MWCNT, polypyrrole with gold nanoparticles hybrid sensing platform.
abstract: An electrochemical immunosensor for the common food pathogen Escherichia coli O157:H7 was developed. This novel immunosensor based on the PPy/AuNP/MWCNT/Chi hybrid bionanocomposite modified pencil graphite electrode (PGE). This hybrid bionanocomposite platform was modified with anti-E. coli O157:H7 monoclonal antibody. The prepared bionanocomposite platform and immunosensor was characterized by using cyclic voltammetry (CV). Under the optimum conditions, the results have shown the order of the preferential selectivity of the method is gram negative pathogenic species E. coli O157:H7. Concentrations of E. coli O157:H7 from 3×101to 3×107cfu/mL could be detected. The detection limit was ∼30cfu/mL in PBS buffer. Briefly, we developed a high sensitive electrochemical immunosensor for specific detection of E. coli O157:H7 contamination with the use of sandwich assay evaluated in this study offered a reliable means of quantification of the bacteria. For the applications in food quality and safety control, our immunosensor showed reproducibility and stability.
authors.affiliation: Vocational School of Health Services, Fatih University, Maltepe, Istanbul 34844, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Güner
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey; Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Çevik
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey. Electronic address: msenel81@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Şenel
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Alpsoy
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D019453:Escherichia coli O157 / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006046:Gold; D048271:Chitosan
keywords: E. coli O157:H7; Gold nanoparticles; Immunosensor; MWCNT; Polypyrrole