title: Inhibition of inflammation induced shedding of the endothelial glycocalyx with low molecular weight heparin.
abstract: The endothelial surface layer (ESL) consists of the endothelial cell (EC) glycocalyx and adsorbed proteins, and forms a barrier between blood and the EC. Enzymatic shedding of the ESL in response to cytokines may expose receptors for leukocyte (WBC) adhesion and increase vascular permeability. Thus, intravital microscopy was used to explore stabilization of the ESL with low molecular weight heparin (LMWH) to mitigate structural changes with inflammation. Following bolus infusions (i.v.) of LMWH (0.12-1.6mg/kg), shedding of glycans in response to 10-7M fMLP was measured by loss of fluorescently labeled lectins bound to the EC and WBC-EC adhesion was monitored in post-capillary venules of rat mesentery. During a 30min exposure to fMLP, a 50% reduction in fluorescence (indicative of glycan shedding) occurred at the lowest dose of LMWH whereas a 50% increase occurred (indicative of ESL compaction) at the highest dose. Shedding was reduced by LMWH in a dose dependent manner with an EC50 of 0.6mg/kg. Concomitant WBC-EC adhesion increased over 3-fold for all doses of LMWH. However, at a dose of 1.6mg/kg, WBC-EC adhesion did not rise significantly during the initial 10min exposure to fMLP. Correlation of WBC adhesion with intensity of the lectin stain for all measurements revealed a significant 40% reduction in adhesion as intensity increased 50%. This relationship was attributed to LMWH inhibition of heparanase and/or binding to components of the glycocalyx that resulted in mitigation of glycan shedding, compaction of the lectin stain and stabilization of the glycocalyx.
authors.affiliation: Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, United States. Electronic address: hhlbio@engr.psu.edu.
authors.forename: Herbert H
authors.initials: HH
authors.lastname: Lipowsky
authors.affiliation: Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, United States.
authors.forename: Anne
authors.initials: A
authors.lastname: Lescanic
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019276:Glycocalyx / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D007249:Inflammation / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000069416:Intravital Microscopy; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011134:Polysaccharides / Q000378:metabolism; D017208:Rats, Wistar; D013997:Time Factors; D014699:Venules / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D006495:Heparin, Low-Molecular-Weight; D011134:Polysaccharides; D009240:N-Formylmethionine Leucyl-Phenylalanine
keywords: Endothelium; Glycocalyx; Heparin; Inflammation; Mesentery; Venules