title: Human BJ Fibroblasts is an Alternative to Mouse BALB/c 3T3 Cells in In Vitro Neutral Red Uptake Assay.
abstract: The OECD GD 129 BALB/c 3T3 neutral red uptake (NRU) assay is a standardized test method for estimating starting dose for an acute oral systemic toxicity test in rodents. Mouse BALB/c 3T3 fibroblasts are the most commonly used cells in the NRU assay. We have previously transferred and validated BALB/c 3T3 NRU assay in our GLP laboratory. Subsequently, in order to obtain more human-relevant cytotoxicity data, we performed an intralaboratory validation using human BJ fibroblasts in the NRU assay instead of mouse BALB/c 3T3 fibroblasts. Here, we present comparative cytotoxicity data of 26 different test chemicals (pharmaceuticals, industrial chemicals, pesticides and food additives) produced with both BALB/c 3T3 NRU and BJ NRU assays.
authors.affiliation: FICAM, School of Medicine, University of Tampere, Tampere, Finland.
authors.forename: Marika
authors.initials: M
authors.lastname: Mannerström
authors.affiliation: FICAM, School of Medicine, University of Tampere, Tampere, Finland.
authors.forename: Tarja
authors.initials: T
authors.lastname: Toimela
authors.affiliation: Cell Biology, School of Medicine, University of Tampere, Tampere, Finland.
authors.forename: Jertta-Riina
authors.initials: JR
authors.lastname: Sarkanen
authors.affiliation: FICAM, School of Medicine, University of Tampere, Tampere, Finland.
authors.forename: Tuula
authors.initials: T
authors.lastname: Heinonen
mesh_terms: D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D041702:BALB 3T3 Cells; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D005347:Fibroblasts; D006801:Humans; D051379:Mice; D009499:Neutral Red / Q000737:chemistry; D023382:Toxicity Tests, Acute / Q000379:methods*
chemical_list: D009499:Neutral Red