title: Correlation analysis of VHL and Jade-1 gene expression in human renal cell carcinoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the correlation of von Hippel-Lindau tumor suppressor (VHL) mRNA expression and jade family PHD finger 1 (Jade-1) gene expression in patients with renal cell carcinoma (RCC). Another aim of this study was to analyze the relationship of these two genes with clinicalpathological features of the RCC patients.
  
  
  METHODS
  A total of 75 RCC patients who received surgically therapy in our hospital were included. All patients had complete pathological data. The expression of VHL/Jade-1 was determined by real-time polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  VHL and Jade-1 were both obviously downregulated in RCC tissues than that of the matched normal tissues, and both negatively correlated with tumor size as well as tumor grade. And we found a fine association of VHL gene expression with Jade-1.
  
  
  CONCLUSIONS
  VHL/Jade-1 exhibited significantly decreased expression in RCC tissues and was closely related to the clinical prognosis of patients. The finding of VHL expression positively correlated with Jade-1 expression shed light and provided crucial evidence on the connection of VHL protein with Wnt/b-catenin pathway.
authors.affiliation: Department of Urology Surgery, The Central Hospital of Lishui City, Lishui, 323000, People's Republic of China.
authors.forename: Wu
authors.initials: W
authors.lastname: Xiao-Fen
authors.affiliation: Department of Urology Surgery, The Central Hospital of Lishui City, Lishui, 323000, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ting
authors.affiliation: Department of Urology Surgery, The Central Hospital of Lishui City, Lishui, 323000, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Jie
authors.affiliation: Department of Urology Surgery, The Central Hospital of Lishui City, Lishui, 323000, People's Republic of China.
authors.forename: Ma
authors.initials: M
authors.lastname: Deng-Yang
authors.affiliation: Department of Urology Surgery, The Central Hospital of Lishui City, Lishui, 323000, People's Republic of China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Qing-Feng
authors.affiliation: Department of Urology Surgery, The Central Hospital of Lishui City, Lishui, 323000, People's Republic of China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xin
keywords: Jade-1; RCC; VHL; Wnt/b-catenin; correlation