title: Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages.
abstract: The endometrial layer of the uterus contains a population of cells with similar characteristics of mesenchymal stem cells (MSCs). In the present study, caprine endometrial mesenchymal stromal stem cells (En-MSCs) characters and differentiation potential to chondrogenic, osteogenic, and adipogenic cell lines as well as their growth kinetics in breeding and anestrous stages were evaluated. En-MSCs were enzymatically isolated from endometrial layer of the uterus of adult goats and were cultured and subcultured until passage 4. The growth kinetics and population doubling time (PDT) of caprine En-MSCs in breeding and anestrous stages were determined. En-MSCs in passage 4 were used for the karyotyping and differentiation into chondrocytes, osteocytes, and adipocytes. The PDT in anestrus phase was 40.6 h and in cyclic goats was 53 h. En-MSCs were fibroblast-like in all passages. The number of chromosomes was normal (2n= 60) with no chromosomal instability. Chondrogenic, osteogenic, and adipogenic differentiation of En-MSCs was confirmed by staining with Alcian blue, Alizarin red, and Oil Red O, respectively. Caprine En-MSCs demonstrated to be an alternative source of MSCs for cell therapy purposes in regenerative medicine.
authors.affiliation: Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amin
authors.identifier: 0000-0002-0222-3035
authors.initials: A
authors.lastname: Tamadon
authors.affiliation: Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.identifier: 0000-0002-5738-1719
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Younes
authors.initials: Y
authors.lastname: Zarezadeh
authors.affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Farhad
authors.identifier: 0000-0001-5086-9087
authors.initials: F
authors.lastname: Rahmanifar
authors.affiliation: Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Human Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dianatpour
authors.affiliation: Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Zare