title: Reducing Caloric Intake Prevents Ischemic Injury and Myocardial Dysfunction and Affects Anesthetic Cardioprotection in Type 2 Diabetic Rats.
abstract: Background. Type 2 diabetes mellitus (T2DM) increases the risk of myocardial ischemia, followed by increased perioperative risk of cardiovascular morbidity. We investigated whether reducing caloric intake reduces ischemic injury and myocardial dysfunction and affects the protective effects of the volatile anesthetic sevoflurane in diet-induced T2DM rats.Methods. Rats received a western (WD) or control diet (CD). Caloric intake was reduced by reversing WD-fed rats to CD. Myocardial function was determined with echocardiography. After 8 weeks of diet feeding, myocardial infarction was induced and the effect of sevoflurane was studied on myocardial function and ischemia/reperfusion injury.Results. WD-feeding resulted in a mild T2DM phenotype and myocardial dysfunction. Sevoflurane further impaired systolic function in WD-fed rats. Unexpectedly, WD-feeding reduced infarct size compared to CD-feeding. Sevoflurane reduced infarct size in CD-fed rats; however it enlarged infarct size in WD-fed rats. Caloric reduction restored myocardial dysfunction and the protective effect of sevoflurane against ischemia compared to WD-fed rats, whereas the protective effects of WD-feeding persisted.Conclusion. Caloric reduction restored the T2DM phenotype and myocardial function, while the cardioprotective properties of WD-feeding or sevoflurane persisted. Our data suggest that reducing caloric intake in T2DM might be a possible intervention to reduce perioperative risk of cardiovascular morbidity.
authors.affiliation: Experimental Laboratory for Vital Signs (ELVIS), Department of Anesthesiology, VU University Medical Center, Amsterdam, Netherlands; Department of Physiology, VU University Medical Center, Amsterdam, Netherlands.
authors.forename: Charissa E
authors.identifier: 0000-0002-0886-1388
authors.initials: CE
authors.lastname: van den Brom
authors.affiliation: Experimental Laboratory for Vital Signs (ELVIS), Department of Anesthesiology, VU University Medical Center, Amsterdam, Netherlands.
authors.forename: Christa
authors.initials: C
authors.lastname: Boer
authors.affiliation: Experimental Laboratory for Vital Signs (ELVIS), Department of Anesthesiology, VU University Medical Center, Amsterdam, Netherlands; Department of Physiology, VU University Medical Center, Amsterdam, Netherlands.
authors.forename: Rob F P
authors.initials: RF
authors.lastname: van den Akker
authors.affiliation: Experimental Laboratory for Vital Signs (ELVIS), Department of Anesthesiology, VU University Medical Center, Amsterdam, Netherlands.
authors.forename: Stephan A
authors.initials: SA
authors.lastname: Loer
authors.affiliation: Experimental Laboratory for Vital Signs (ELVIS), Department of Anesthesiology, VU University Medical Center, Amsterdam, Netherlands.
authors.forename: R Arthur
authors.initials: RA
authors.lastname: Bouwman
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D031204:Caloric Restriction; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D066273:Diet, Western; D004452:Echocardiography; D002149:Energy Intake; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D002316:Cardiotonic Agents; D008738:Methyl Ethers; D000077149:Sevoflurane