title: Comparison of thein VitroUptake and Toxicity of Collagen- and Synthetic Polymer-Coated Gold Nanoparticles.
abstract: We studied the physico-chemical properties (size, shape, zeta-potential), cellular internalization and toxicity of gold nanoparticles (NPs) stabilized with the most abundant mammalian protein, collagen. The properties of these gold NPs were compared to the same sized gold NPs coated with synthetic poly(isobutylene-alt-maleic anhydride) (PMA). Intracellular uptake and cytotoxicity were assessed in two cell lines (cervical carcinoma and lung adenocarcinoma cells) by employing inductively-coupled plasma-mass spectrometry (ICP-MS) analysis and a cell viability assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), respectively. We found that the collagen-coated gold NPs exhibit lower cytotoxicity, but higher uptake levels than PMA-coated gold NPs. These results demonstrate that the surface coating of Au NPs plays a decisive role in their biocompatibility.
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. nicolae.leopold@phys.ubbcluj.ro.|Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca 400084, Romania. nicolae.leopold@phys.ubbcluj.ro.
authors.forename: Oana T
authors.initials: OT
authors.lastname: Marisca
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. kantnerk@staff.uni-marburg.de.
authors.forename: Karsten
authors.initials: K
authors.lastname: Kantner
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. pfeiffer-christian@gmx.net.
authors.forename: Christian
authors.initials: C
authors.lastname: Pfeiffer
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. qian.zhang@physik.uni-marburg.de.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. beatriz.pelazgarcia@physik.uni-marburg.de.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pelaz
authors.affiliation: Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca 400084, Romania. nicolae.leopold@phys.ubbcluj.ro.
authors.forename: Nicolae
authors.initials: N
authors.lastname: Leopold
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. wolfgang.parak@physik.uni-marburg.de.|CIC Biomagune, San Sebastian 20009, Spain. wolfgang.parak@physik.uni-marburg.de.
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany. joanna.rejman@med.uni-muenchen.de.|Dr. von Hauner Children's Hospital, Ludwig Maximilians University, Munich 80337, Germany. joanna.rejman@med.uni-muenchen.de.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rejman
keywords: biocompatibility; cellular uptake; gold nanoparticles; toxicity