title: Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.
abstract: Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labour than in single immunoassays. This chapter details the methods to develop and manufacture a 5-plex immunoassay for the Luminex® platform. Although assay development is not included here, the same methods can be used to covalently couple antibodies to the Luminex beads and to label antibodies for the screening of sandwich pairs, if needed. An example will be given for the analysis of five hormones (glucagon-like peptide 1, growth hormone, insulin, leptin and thyroid-stimulating hormone) in serum samples from schizophrenia patients and controls.
authors.affiliation: Ampersand Biosciences, 3 Main Street, Saranac Lake, NY, USA. lstephen@ampersandbio.com.
authors.forename: Laurie
authors.initials: L
authors.lastname: Stephen
authors.affiliation: Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Schwarz
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária ZeferinoVaz, 13083-862, Campinas, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D019921:Biotinylation; D001798:Blood Proteins / Q000032:analysis; D016207:Cytokines / Q000097:blood; D005260:Female; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006728:Hormones / Q000097:blood; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D018189:Immunomagnetic Separation; D007202:Indicators and Reagents; D007834:Lasers; D008297:Male; D008863:Microspheres; D011933:Reagent Kits, Diagnostic; D012559:Schizophrenia / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D016207:Cytokines; D006728:Hormones; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic
keywords: Antibody; Biomarker; Disease; Hormone; Luminex assay; Multiplex assay; Schizophrenia