title: Reduction of greenhouse gases emissions during anoxic wastewater treatment by strengthening nitrite-dependent anaerobic methane oxidation process.
abstract: Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered process performed by NC10 phylum, which plays an important role in greenhouse gases (GHG) reduction. In this study, co-existence of n-damo bacteria and methanogens was successfully achieved by using upflow anaerobic sludge blanket (UASB) reactor. Reactor with inorganic carbon source (CO2/H2) showed the highest abundance of n-damo bacteria and the highest n-damo potential activity, resulted in its highest nitrogen removal rate. Significant reduction in GHG was obtained after introduction of n-damo process, especially for N2O. Furthermore, GHG emissions decreased with the increase of n-damo bacteria abundance. Community structure analysis found carbon source could influence the diversity of n-damo bacteria indirectly. And phylogenetic analysis showed that all the obtained sequences were assigned to group B, mainly due to in situ production and consumption of CH4.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Ma
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China. Electronic address: huzhen885@sdu.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ma
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Ru
mesh_terms: D000693:Anaerobiosis; D005740:Gases; D008697:Methane; D009573:Nitrites; D010084:Oxidation-Reduction; D010802:Phylogeny; D062065:Wastewater
chemical_list: D005740:Gases; D008697:Methane; D009573:Nitrites; D062065:Wastewater
keywords: Anoxic wastewater treatment; Carbon sources; Greenhouse gases; Nitrite-dependent anaerobic methane-oxidation; Reduction