title: Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells.
abstract: Hair follicles develop from the ectoderm in embryos and cyclically regenerate using proper spatiotemporal signaling molecules, which are conserved in organogenesis during adulthood. Previously, we demonstrated that bioengineered hair follicle germs could regenerate functional hair follicles via a three-dimensional cell manipulation technique, which we named the "organ germ method ." We could also regulate the type of hair follicle and pigmentation with correct structures by rearranging the source of the cells. In this article, we describe a detailed protocol for the regeneration of functional hair follicles and their stem cell niches by the rearrangement of embryonic or adult hair follicle-derived epithelial and mesenchymal cells.
authors.affiliation: Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Asakawa
authors.affiliation: Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe, Hyogo, 650-0047, Japan.|Organ Technologies Inc., Minato-ku, Tokyo, 108-0074, Japan.
authors.forename: Koh-Ei
authors.initials: KE
authors.lastname: Toyoshima
authors.affiliation: Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuou-ku, Kobe, Hyogo, 650-0047, Japan. t-tsuji@cdb.riken.jp.|Organ Technologies Inc., Minato-ku, Tokyo, 108-0074, Japan. t-tsuji@cdb.riken.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000166:cytology; D018859:Hair Follicle / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D038081:Organogenesis / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods
keywords: Bioengineered hair follicle; Organ culture; Organ germ method; Regeneration; Transplantation