title: Effects of Vitamin B6 Deficiency on the Composition and Functional Potential of T Cell Populations.
abstract: The immune system is critical in preventing infection and cancer, and malnutrition can weaken different aspects of the immune system to undermine immunity. Previous studies suggested that vitamin B6 deficiency could decrease serum antibody production with concomitant increase in IL4 expression. However, evidence on whether vitamin B6 deficiency would impair immune cell differentiation, cytokines secretion, and signal molecule expression involved in JAK/STAT signaling pathway to regulate immune response remains largely unknown. The aim of this study is to investigate the effects of vitamin B6 deficiency on the immune system through analysis of T lymphocyte differentiation, IL-2, IL-4, and INF-γsecretion, andSOCS-1andT-betgene transcription. We generated a vitamin B6-deficient mouse model via vitamin B6-depletion diet. The results showed that vitamin B6 deficiency retards growth, inhibits lymphocyte proliferation, and interferes with its differentiation. After ConA stimulation, vitamin B6 deficiency led to decrease in IL-2 and increase in IL-4 but had no influence on IFN-γ. Real-time PCR analysis showed that vitamin B6 deficiency downregulatedT-betand upregulatedSOCS-1transcription. This study suggested that vitamin B6 deficiency influenced the immunity in organisms. Meanwhile, the appropriate supplement of vitamin B6 could benefit immunity of the organism.
authors.affiliation: Department of Nutrition and Health, Research Centre of Biomedical Technology Co. Ltd., Yancheng Vocational Institute of Health Sciences (YIHS), Jiangsu 224005, China; Research Center for Food Safety and Nutrition, Key Lab of Urban Agriculture (South), Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Bingjun
authors.initials: B
authors.lastname: Qian
authors.affiliation: Research Center for Food Safety and Nutrition, Key Lab of Urban Agriculture (South), Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shanqi
authors.initials: S
authors.lastname: Shen
authors.affiliation: Research Center for Food Safety and Nutrition, Key Lab of Urban Agriculture (South), Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Research Center for Food Safety and Nutrition, Key Lab of Urban Agriculture (South), Bor S. Luh Food Safety Research Center, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Pu
authors.identifier: 0000-0001-8204-7475
authors.initials: P
authors.lastname: Jing
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016207:Cytokines / Q000235:genetics*; D004032:Diet; D015536:Down-Regulation; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000097:blood; D000071222:Suppressor of Cytokine Signaling 1 Protein / Q000235:genetics; D020825:T-Box Domain Proteins / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology*; D026681:Vitamin B 6 Deficiency / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D014977:Xanthurenates / Q000097:blood; D000098815:T-bet Transcription Factor
chemical_list: D016207:Cytokines; D007376:Interleukin-2; C491590:Socs1 protein, mouse; D000071222:Suppressor of Cytokine Signaling 1 Protein; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014977:Xanthurenates; D015847:Interleukin-4; C028330:xanthurenic acid; D011732:Pyridoxal Phosphate; D007371:Interferon-gamma; C024593:pyridoxine 5-phosphate