title: Induction of PINK1/Parkin-Mediated Mitophagy.
abstract: PINK1/Parkin mitophagy is a key mechanism to contribute mitochondrial quality control, and the defects are thought to be a cause of those Parkinson's disease onsets. Upon loss of mitochondrial membrane potential, PINK1 and Parkin are activated to promote the proteasomal degradation of mitochondrial outer membrane proteins and selective elimination of damaged mitochondria by autophagy. In this chapter, we describe the methods for induction of PINK1/Parkin-mediated mitophagy in tissue culture cell lines.
authors.affiliation: Department of Neurology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, 113-8421, Japan.
authors.forename: Shigeto
authors.initials: S
authors.lastname: Sato
authors.affiliation: Department of Neurology, Juntendo University School of Medicine, Hongo, Bunkyo, Tokyo, Japan. nohuruya@juntendo.ac.jp.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Furuya
mesh_terms: D000818:Animals; D001343:Autophagy; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism*; D000099202:PTEN-Induced Putative Kinase
chemical_list: D011494:Protein Kinases; D044767:Ubiquitin-Protein Ligases; D000099202:PTEN-Induced Putative Kinase; C111567:parkin protein
keywords: Mitochondrial membrane potential; PINK1; Parkin