title: MicroRNA-328 is involved in wound repair process in human bronchial epithelial cells.
abstract: Our aim was to investigate the role of microRNA on epithelial wound repair by global microRNA silencing. We have also analysed the influence of five miRNAs (miR-328, miR-342, miR-411, miR-609, miR-888, previously identified) on wound repair in 16HBE14o-bronchial epithelial cell line. Cells were transfected with siRNAs against human DROSHA and DICER1 or miRNA mimics or inhibitors. Wounding assays were performed and the cells were observed using time-lapse microscopy. The area of damage was calculated at chosen time points, followed by data analysis. Cells with silenced global miRNA expression showed a significantly slower repair rate compared to the control cells (p=0.001). For miR-328, we observed significantly delayed repair in cells transfected with the inhibitor compared to control (p=0.02). Global microRNA silencing significantly decreased the repair rate of airway epithelial cells in vitro, indicating an important role of miRNA in the regulation of wound repair and that miR-328, possibly involved in actin pathway, may be a potent modifier of this process.
authors.affiliation: Laboratory of Molecular and Cell Biology, Department of Pediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 27/33 Szpitalna St., 60-572 Poznan, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Narożna
authors.affiliation: Laboratory of Molecular and Cell Biology, Department of Pediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 27/33 Szpitalna St., 60-572 Poznan, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Langwinski
authors.affiliation: Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Jackson
authors.affiliation: Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Lackie
authors.affiliation: Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, UK.
authors.forename: John W
authors.initials: JW
authors.lastname: Holloway
authors.affiliation: Laboratory of Molecular and Cell Biology, Department of Pediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 27/33 Szpitalna St., 60-572 Poznan, Poland. Electronic address: alszczep@ump.edu.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szczepankiewicz
mesh_terms: D000704:Analysis of Variance; D001980:Bronchi / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D014162:Transfection; D014945:Wound Healing / Q000502:physiology*
chemical_list: C548170:MIRN328 microRNA, human; C582326:MIRN411 microRNA, human; C000589534:MIRN888 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: Epithelial cells; Gene silencing; Pathway analysis; Time-lapse microscopy; Wound repair; miRNA