title: Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae).
abstract: RNA interference has been used to study insects' gene function and regulation. Glycogen synthase (GS) and glycogen phosphorylase (GP) are two key enzymes in carbohydrates' conversion in insects. Glycogen content and GP and GS gene expression in several tissues and developmental stages of the Brown planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) were analyzed in the present study, using quantitative reverse-transcription polymerase chain reaction to determine their response to double-stranded trehalases (dsTREs), trehalose-6-phosphate synthases (dsTPSs), and validamycin injection. The highest expression of both genes was detected in the wing bud, followed by leg and head tissues, and different expression patterns were shown across the developmental stages analyzed. Glycogen content significantly decreased 48 and 72 h after dsTPSs injection and 48 h after dsTREs injection. GP expression increased 48 h after dsTREs and dsTPSs injection and significantly decreased 72 h after dsTPSs, dsTRE1-1, and dsTRE1-2 injection. GS expression significantly decreased 48 h after dsTPS2 and dsTRE2 injection and 72 h after dsTRE1-1 and dsTRE1-2 injection. GP and GS expression and glycogen content significantly decreased 48 h after validamycin injection. The GP activity significantly decreased 48 h after validamycin injection, while GS activities of dsTPS1 and dsTRE2 injection groups were significantly higher than that of double-stranded GFP (dsGFP) 48 h after injection, respectively. Thus, glycogen is synthesized, released, and degraded across several insect tissues according to the need to maintain stable trehalose levels.
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China (zhanglu_1140@163.com; whjj_701@163.com; jarrison@163.com; shenqida@yeah.net; sgwang@mail.hz.zj.cn).
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China (zhanglu_1140@163.com; whjj_701@163.com; jarrison@163.com; shenqida@yeah.net; sgwang@mail.hz.zj.cn).
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China (zhanglu_1140@163.com; whjj_701@163.com; jarrison@163.com; shenqida@yeah.net; sgwang@mail.hz.zj.cn).
authors.forename: Jianyi
authors.initials: J
authors.lastname: Chen
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China (zhanglu_1140@163.com; whjj_701@163.com; jarrison@163.com; shenqida@yeah.net; sgwang@mail.hz.zj.cn).
authors.forename: Qida
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China (zhanglu_1140@163.com; whjj_701@163.com; jarrison@163.com; shenqida@yeah.net; sgwang@mail.hz.zj.cn).
authors.forename: Shigui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021, China (xu 13588332930@163.com).
authors.forename: Hongxing
authors.initials: H
authors.lastname: Xu
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China (zhanglu_1140@163.com; whjj_701@163.com; jarrison@163.com; shenqida@yeah.net; sgwang@mail.hz.zj.cn).
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
mesh_terms: D000818:Animals; D015870:Gene Expression; D005964:Glucosyltransferases / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000235:genetics* / Q000378:metabolism; D006006:Glycogen Synthase / Q000235:genetics* / Q000378:metabolism; D006430:Hemiptera / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007294:Inositol / Q000031:analogs & derivatives; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008845:Microinjections; D009928:Organ Specificity; D034622:RNA Interference; D014198:Trehalase / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D007294:Inositol; C003749:validamycins; D006003:Glycogen; D014199:Trehalose; D005964:Glucosyltransferases; D024981:Glycogen Phosphorylase; D006006:Glycogen Synthase; C023180:trehalose-6-phosphate synthase; D014198:Trehalase
keywords: Nilaparvata lugens; RNA interference; glycogen; glycogen phosphorylase; glycogen synthase