title: Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing.
abstract: An ideal wound dressing can both promote wound healing and prevent bacterial infection. Here, we report a potential dressing prepared by incorporating an optimized concentration of silver nanoparticles (AgNPs) into the microfibers of a natural eggshell membrane (EM) using environmentally friendly and mussel-inspired dopamine. Briefly, acid-treated EM was used as a porous membrane for polydopamine-reduced AgNPs synthesis. To obtain the optimal cytocompatible silver concentration, cellular attachment and MTT assay were performed with different concentrations of AgNPs. The morphology of the EM and AgNPs was confirmed by scanning electronic microscopy, scanning transmission electronic microscopy and Fourier transform infrared spectroscopy. The synthesized EM/AgNPs exhibited steady and safe AgNPs release, which was further tested for antibacterial activity against Escherichia coli and Staphylococcus aureus by disc diffusion method and bacterial suspension assay. Finally, in a murine full-thickness skin wound model, we found that EM/AgNPs could promote re-epithelialization, granulation tissue formation and wound healing via enhancing cell proliferation, as demonstrated by the expression of proliferating cell nuclear antigen (PCNA), and controlling inflammation response, as demonstrated by the expression of interleukin-1β (IL-1β). These findings suggest that EM/AgNPs may have a promising application in wound management.
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Menglong
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Tan
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China. malcolm.xing@umanitoba.ca.|Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.|Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.|Children's Hospital Research Institute of Manitoba, Winnipeg, Canada. malcolm.xing@umanitoba.ca.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China. editorinchief@burninchina.com.|Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China. editorinchief@burninchina.com.|Department of Plastic and Burn Surgery, the First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, China. editorinchief@burninchina.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D012834:Silver / Q000008:administration & dosage* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000517:prevention & control; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver