title: Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum.
abstract: The National Institute of Standards and Technology (NIST) has developed Standard Reference Material (SRM) 972a Vitamin D Metabolites in Frozen Human Serum as a replacement for SRM 972, which is no longer available. SRM 972a was developed in collaboration with the National Institutes of Health's Office of Dietary Supplements. In contrast to the previous reference material, three of the four levels of SRM 972a are composed of unmodified human serum. This SRM has certified and reference values for the following 25-hydroxyvitamin D [25(OH)D] species: 25(OH)D2, 25(OH)D3, and 3-epi-25(OH)D3. The value assignment and certification process included three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). The value assignment methods employed have been modified from those utilized for the previous SRM, and all three approaches now incorporate chromatographic resolution of the stereoisomers, 25(OH)D3and 3-epi-25(OH)D3.
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Karen W
authors.identifier: 0000-0001-7540-5860
authors.initials: KW
authors.lastname: Phinney
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Susan S-C
authors.initials: SS
authors.lastname: Tai
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Mary
authors.initials: M
authors.lastname: Bedner
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Johanna E
authors.initials: JE
authors.lastname: Camara
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Rosalind R C
authors.initials: RRC
authors.lastname: Chia
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Lane C
authors.initials: LC
authors.lastname: Sander
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Sharpless
authors.affiliation: Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Wise
authors.affiliation: Statistical Engineering Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors.forename: James H
authors.initials: JH
authors.lastname: Yen
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , Atlanta, Georgia 30341, United States.
authors.forename: Rosemary L
authors.initials: RL
authors.lastname: Schleicher
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , Atlanta, Georgia 30341, United States.
authors.forename: Madhulika
authors.initials: M
authors.lastname: Chaudhary-Webb
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , Atlanta, Georgia 30341, United States.
authors.forename: Khin L
authors.initials: KL
authors.lastname: Maw
authors.affiliation: Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , Atlanta, Georgia 30341, United States.
authors.forename: Yasamin
authors.initials: Y
authors.lastname: Rahmani
authors.affiliation: Office of Dietary Supplements, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Betz
authors.affiliation: Office of Dietary Supplements, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Joyce
authors.initials: J
authors.lastname: Merkel
authors.affiliation: Office of Dietary Supplements, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Sempos
authors.affiliation: Office of Dietary Supplements, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Coates
authors.affiliation: Department of Public Health Sciences, Stritch School of Medicine, Loyola University Chicago , Maywood, Illinois 60153, United States.
authors.forename: Ramón A
authors.initials: RA
authors.lastname: Durazo-Arvizu
authors.affiliation: Bureau of Nutritional Sciences, Health Canada , Ottawa, Ontario Canada K1A 0K9.
authors.forename: Kurtis
authors.initials: K
authors.lastname: Sarafin
authors.affiliation: Bureau of Nutritional Sciences, Health Canada , Ottawa, Ontario Canada K1A 0K9.
authors.forename: Stephen P J
authors.initials: SPJ
authors.lastname: Brooks
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000097:blood* / Q000592:standards; D002112:Calcifediol / Q000097:blood* / Q000737:chemistry / Q000592:standards; D002853:Chromatography, Liquid / Q000592:standards*; D006801:Humans; D013058:Mass Spectrometry / Q000592:standards*; D012015:Reference Standards; D012016:Reference Values; D013237:Stereoisomerism; D014481:United States; D037041:United States Government Agencies
chemical_list: D015652:25-Hydroxyvitamin D 2; D002112:Calcifediol