title: Improved Neural Regeneration with Olfactory Ensheathing Cell Inoculated PLGA Scaffolds in Spinal Cord Injury Adult Rats.
abstract: Every year, around the world, between 250000 and 500000 people suffer from spinal cord injury (SCI). This study investigated the potential for poly (lactic-co-glycolic acid) (PLGA) complex inoculated with olfactory ensheathing cells (OECs) to treat spinal cord injury in a rat model.
  
  
  
  OECs were identified by immunofluorescence based on the nerve growth factor receptor (NGFR) p75. The Basso, Beattie, and Bresnahan (BBB) score, together with an inclined plane (IP) test were used to detect functional recovery. Nissl staining along with the luxol fast blue (LFB) staining were independently employed to illustrate morphological alterations. More so, immunofluorescence labeling of the glial fibrillary acidic protein (GFAP) and the microtubule-associated protein-2 (MAP-2), representing astrocytes and neurons respectively, were investigated at time points of weeks 2 and 8 post-operation.
  
  
  
  The findings showed enhanced locomotor recovery, axon myelination and better protected neurons post SCI when compared with either PLGA or untreated groups (P < 0.05).
  
  
  
  PLGA complexes inoculated with OECs improve locomotor functional recovery in transected spinal cord injured rat models, which is most likely due to the fact it is conducive to a relatively benevolent microenvironment, has nerve protective effects, as well as the ability to enhance remyelination, via a promotion of cell differentiation and inhibition of astrocyte formation.
authors.affiliation: Department of Orthopedics, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Changxing
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, Hangzhou Red Cross Hospital, Hangzhou, China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Huo
authors.affiliation: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Histology and Embryology, Shaoxing University School of Medicine, Shaoxing, China.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Benson O A
authors.initials: BOA
authors.lastname: Botchway
authors.affiliation: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Henry
authors.initials: H
authors.lastname: Davies
authors.affiliation: Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Marong
authors.initials: M
authors.lastname: Fang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D054457:Tissue Scaffolds
chemical_list: D005904:Glial Fibrillary Acidic Protein; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
keywords: Functional recovery; Olfactory ensheathing cells; Poly (lactic-co-glycolic acid) complex; Spinal cord injury