title: Development of certified reference materials for accurate determination of fluoroquinolone antibiotics in chicken meat.
abstract: Certified reference materials (CRMs; KRISS CRM 108-03-003, 108-03-004) were developed for the accurate determination of fluoroquinolones (enrofloxacin and ciprofloxacin, respectively) in chicken meat. Two groups of chickens were cured with feeds containing enrofloxacin and ciprofloxacin, respectively. After slaughter, the thigh and breast meats were combined for the respective groups and the meat was freeze-dried, pulverized, sieved, and V-mixed. The final bulk material was bottled in 10g portions. For certification of the CRMs, isotope dilution-liquid chromatography/tandem mass spectrometry was used. The certified values of the CRMs were (19.06±0.86)mg/kg for enrofloxacin and (1.095±0.038)mg/kg for ciprofloxacin. The stabilities of the CRMs were monitored at -70°C for 12months, at -20°C for 2months, and at room temperature for 1month. Both CRM candidates were stable during the monitoring period for each temperature.
authors.affiliation: Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Yuseong, Daejeon 34113, South Korea.
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Hyung
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, South Korea.
authors.forename: Chi-Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Yuseong, Daejeon 34113, South Korea. Electronic address: byungjoo@kriss.re.kr.
authors.forename: Byungjoo
authors.initials: B
authors.lastname: Kim
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000592:standards; D002645:Chickens; D002853:Chromatography, Liquid; D002939:Ciprofloxacin / Q000032:analysis* / Q000592:standards; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis* / Q000592:standards; D005504:Food Analysis; D007554:Isotopes; D011200:Poultry; D012015:Reference Standards; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D007554:Isotopes; D000077422:Enrofloxacin; D002939:Ciprofloxacin
keywords: Certified reference material; Chicken meat; Fluoroquinolones; ID-LC/MS/MS