title: Specific expression of novel long non-coding RNAs in high-hyperdiploid childhood acute lymphoblastic leukemia.
abstract: Pre-B cell childhood acute lymphoblastic leukemia (pre-B cALL) is a heterogeneous disease involving many subtypes typically stratified using a combination of cytogenetic and molecular-based assays. These methods, although widely used, rely on the presence of known chromosomal translocations, which is a limiting factor. There is therefore a need for robust, sensitive, and specific molecular biomarkers unaffected by such limitations that would allow better risk stratification and consequently better clinical outcome. In this study we performed a transcriptome analysis of 56 pre-B cALL patients to identify expression signatures in different subtypes. In both protein-coding and long non-coding RNAs (lncRNA), we identified subtype-specific gene signatures distinguishing pre-B cALL subtypes, particularly in t(12;21) and hyperdiploid cases. The genes up-regulated in pre-B cALL subtypes were enriched in bivalent chromatin marks in their promoters. LncRNAs is a new and under-studied class of transcripts. The subtype-specific nature of lncRNAs suggests they may be suitable clinical biomarkers to guide risk stratification and targeted therapies in pre-B cALL patients.
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Mathieu
authors.identifier: http://orcid.org/0000-0001-9162-086X
authors.initials: M
authors.lastname: Lajoie
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Simon
authors.initials: S
authors.lastname: Drouin
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Maxime
authors.initials: M
authors.lastname: Caron
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Pascal
authors.initials: P
authors.lastname: St-Onge
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Manon
authors.identifier: http://orcid.org/0000-0003-4729-6550
authors.initials: M
authors.lastname: Ouimet
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Romain
authors.initials: R
authors.lastname: Gioia
authors.affiliation: Mathematics and Statistics Department, University of Quebec at Montreal (UQAM), 201 President-Kennedy Av., Montreal, QC, Canada.
authors.forename: Marie-Hélène
authors.identifier: http://orcid.org/0000-0002-6047-4086
authors.initials: MH
authors.lastname: Lafond
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Ramon
authors.identifier: http://orcid.org/0000-0003-4217-2014
authors.initials: R
authors.lastname: Vidal
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.
authors.forename: Chantal
authors.initials: C
authors.lastname: Richer
authors.affiliation: Mathematics and Statistics Department, University of Quebec at Montreal (UQAM), 201 President-Kennedy Av., Montreal, QC, Canada.
authors.forename: Karim
authors.initials: K
authors.lastname: Oualkacha
authors.affiliation: Department of Endocrinology and Nephrology, Laval University, 2705 Laurier Blvd., Quebec City, QC, Canada.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Droit
authors.affiliation: Division of Hematology-Oncology, Research Center, Sainte-Justine University Health Center, 3175 Chemin de la Côte-Sainte-Catherine, Montréal, QC, Canada.|Department of Pediatrics, Faculty of Medicine, University of Montreal, Montreal, QC, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sinnett
mesh_terms: D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D008297:Male; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D054448:Precursor Cells, B-Lymphoid / Q000378:metabolism / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding