title: Proteome Profiling of Wheat Shoots from Different Cultivars.
abstract: Wheat is a cereal grain and one of the world's major food crops. Recent advances in wheat genome sequencing are by now facilitating its genomic and proteomic analyses. However, little is known about possible differences in total protein levels of hexaploid versus tetraploid wheat cultivars, and also knowledge of phosphorylated wheat proteins is still limited. Here, we performed a detailed analysis of the proteome of seedling leaves from two hexaploid wheat cultivars (Triticum aestivumL. Pavon 76 and USU-Apogee) and one tetraploid wheat (T. turgidumssp.durumcv. Senatore Cappelli). Our shotgun proteomics data revealed that, whereas we observed some significant differences, overall a high similarity between hexaploid and tetraploid varieties with respect to protein abundance was observed. In addition, already at the seedling stage, a small set of proteins was differential between the small (USU-Apogee) and larger hexaploid wheat cultivars (Pavon 76), which could potentially act as growth predictors. Finally, the phosphosites identified in this study can be retrieved from the in-house developed plant PTM-Viewer (bioinformatics.psb.ugent.be/webtools/ptm_viewer/), making this the first searchable repository for phosphorylated wheat proteins. This paves the way for further in depth, quantitative (phospho)proteome-wide differential analyses upon a specific trigger or environmental change.
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium; Center for Plant Systems Biology, VIBGhent, Belgium; Medical Biotechnology Center, VIBGhent, Belgium; Department of Biochemistry, Ghent UniversityGhent, Belgium.
authors.forename: Lam Dai
authors.initials: LD
authors.lastname: Vu
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium; Center for Plant Systems Biology, VIBGhent, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Verstraeten
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium; Center for Plant Systems Biology, VIBGhent, Belgium; Medical Biotechnology Center, VIBGhent, Belgium; Department of Biochemistry, Ghent UniversityGhent, Belgium.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Stes
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium; Center for Plant Systems Biology, VIBGhent, Belgium.
authors.forename: Michiel
authors.initials: M
authors.lastname: Van Bel
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium; Center for Plant Systems Biology, VIBGhent, Belgium.
authors.forename: Frederik
authors.initials: F
authors.lastname: Coppens
authors.affiliation: Medical Biotechnology Center, VIBGhent, Belgium; Department of Biochemistry, Ghent UniversityGhent, Belgium.
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.affiliation: Department of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgium; Center for Plant Systems Biology, VIBGhent, Belgium.
authors.forename: Ive
authors.initials: I
authors.lastname: De Smet
keywords: leaf; mass spectrometry; phosphoproteome; proteome; wheat