title: Bioinformatic identification of candidate genes induced by trichostatin A in BGC-823 gastric cancer cells.
abstract: The aim of the present study was to identify the candidate genes induced by trichostatin A (TSA) in BGC-823 gastric cancer (GC) cells and to explore the possible inhibition mechanism of TSA in GC. Gene expression data were obtained through chip detection, and differentially expressed genes (DEGs) between GC cells treated with TSA and untreated GC cells (control group) were identified. Gene ontology analysis of the DEGs was performed using the database for annotation, visualization and integrated discovery. Then sub-pathway enrichment analysis was performed and a microRNA (miRNA) regulatory network was constructed. We selected 76 DEGs, among which 43 were downregulated genes and 33 were upregulated genes. By sub-pathway enrichment analysis of the DEGs, the propanoate metabolism pathway was selected as the sub-pathway. By constructing a miRNA regulatory network, we identified thatDKK1andKLF13were the top hub nodes. The propanoate metabolism pathway and the genesDKK1andKLF13may play significant roles in the inhibition of GC induced by TSA. These genes may be potential therapeutic targets for GC. However, further experiments are still required to confirm our results.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150080, P.R. China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150080, P.R. China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150080, P.R. China.
authors.forename: Chunfa
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors.forename: Riheng
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Surgery, Traditional Chinese Medicine Hospital of Acheng, Harbin, Heilongjiang 150300, P.R. China.
authors.forename: Longbin
authors.initials: L
authors.lastname: Shang
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150080, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zou
keywords: differentially expressed gene; gastric cancer; therapeutic targets; trichostatin A