title: Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation.
abstract: SeveralSaccharomyces cerevisiaestrains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this study, we identify abundant proteins in the formation of the biofilm of an industrial flor yeast strain. A database search to enrich flor yeast "biological process" and "cellular component" according to Gene Ontology Terminology (GO Terms) and, "pathways" was carried out. The most abundant proteins detected were largely involved in respiration, translation, stress damage prevention and repair, amino acid metabolism (glycine, isoleucine, leucine and arginine), glycolysis/gluconeogenesis and biosynthesis of vitamin B9 (folate). These proteins were located in cellular components as in the peroxisome, mitochondria, vacuole, cell wall and extracellular region; being these two last directly related with the flor formation. Proteins like Bgl2p, Gcv3p, Hyp2p, Mdh1p, Suc2p and Ygp1p were quantified in very high levels. This study reveals some expected processes and provides new and important information for the design of conditions and genetic constructions of flor yeasts for improving the cellular survival and, thus, to optimize biological aging of Sherry wine production.
authors.affiliation: Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, 14014-Cordoba, Spain. b62mogaj@uco.es.
authors.forename: Jaime
authors.initials: J
authors.lastname: Moreno-García
authors.affiliation: Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, 14014-Cordoba, Spain. mi1gamaj@uco.es.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Mauricio
authors.affiliation: Department of Agricultural Chemistry, Agrifood Campus of International Excellence ceiA3, University of Cordoba, 14014-Cordoba, Spain. qe1movij@uco.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Moreno
authors.affiliation: Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, 14014-Cordoba, Spain. mi2gamam@uco.es.
authors.forename: Teresa
authors.initials: T
authors.lastname: García-Martínez
mesh_terms: D018441:Biofilms; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D053858:Metabolic Networks and Pathways; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: GO Terms; LTQ Orbitrap XL MS; OFFGEL electrophoresis; Saccharomyces cerevisiae; flor velum