title: Synaptic vesicles isolated from the electric organ ofTorpedo californicaand from the central nervous system ofMus musculuscontain small ribonucleic acids (sRNAs).
abstract: Synaptic vesicles (SVs) are presynaptic organelles that load and release small molecule neurotransmitters at chemical synapses. In addition to classic neurotransmitters, we have demonstrated that SVs isolated from the Peripheral Nervous Systems (PNS) of the electric organ ofTorpedo californica,a model cholinergic synapse, and SVs isolated from the Central Nervous System (CNS) ofMus musculus(mouse) contain small ribonucleic acids (sRNAs; ≤ 50 nucleotides) (Scientific Reports, 5:1-14(14918) Li et al. (2015) [1]). Our previous publication provided the five most abundant sequences associated with theT. californicaSVs, and the ten most abundant sequences associated with the mouse SVs, representing 59% and 39% of the total sRNA reads sequenced, respectively). We provide here a full repository of the SV sRNAs sequenced fromT. californicaand the mouse deposited in the NCBI as biosamples. Three data studies are included: SVs isolated from the electric organ ofT. californicausing standard techniques, SVs isolated from the electric organ ofT. californicausing standard techniques with an additional affinity purification step, and finally, SVs isolated from the CNS of mouse. The three biosamples are available at https://www.ncbi.nlm.nih.gov/biosample/ SRS1523467, SRS1523466, and SRS1523472 respectively.
authors.affiliation: Department of Biology, Texas A&M University, TAMU 3258, College Station, TX 77843-3474, United States.
authors.forename: Huinan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biology, Texas A&M University, TAMU 3258, College Station, TX 77843-3474, United States.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Biology, Texas A&M University, TAMU 3258, College Station, TX 77843-3474, United States.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Aramayo
authors.affiliation: Department of Biology, Texas A&M University, TAMU 3258, College Station, TX 77843-3474, United States.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Sachs
authors.affiliation: Department of Biology, Texas A&M University, TAMU 3258, College Station, TX 77843-3474, United States.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Harlow
keywords: Micro-RNA; Mus musculus; Synaptic vesicle; Torpedo californica; tRNA fragment