title: Temozolomide-Mediated Apoptotic Death Is Improved by Thymoquinone in U87MG Cell Line.
abstract: Apoptosis induction of cancer cells can be an appropriate strategy by which chemotherapeutic agents kill tumor cells. The aim of the present study was to investigate the effect of temozolomide and thymoquinone combination on apoptotic pathway of human glioblastoma multiforme cell line (U87MG). U87MG cells were cultured, treated with temozolomide and thymoquinone, and cell proliferation was measured. Apoptosis cell death and its possible mechanism were investigated by various methods. Combination of temozolomide and thymoquinone had a synergistic effect on cells viability. Thymoquinone intensified the temozolomide-induced apoptosis. Combination of temozolomide and thymoquinone can be a good strategy for treatment of glioblastoma.
authors.affiliation: a Fertility and Infertility Research Center , Kermanshah University of Medical Sciences , Kermanshah , Iran.
authors.forename: Mozafar
authors.initials: M
authors.lastname: Khazaei
authors.affiliation: a Fertility and Infertility Research Center , Kermanshah University of Medical Sciences , Kermanshah , Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Pazhouhi
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D016227:Benzoquinones / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D020552:Comet Assay; D045304:Cytochromes c / Q000378:metabolism; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D004357:Drug Synergism; D005909:Glioblastoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D000077204:Temozolomide
chemical_list: D016227:Benzoquinones; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D003606:Dacarbazine; D045304:Cytochromes c; D005978:Glutathione; C003466:thymoquinone; D000077204:Temozolomide
keywords: Glioblastoma multiforme; apoptosis; temozolomide; thymoquinone