title: Hydrogel containing silibinin-loaded pomegranate oil based nanocapsules exhibits anti-inflammatory effects on skin damage UVB radiation-induced in mice.
abstract: The present study shows the development of a topical formulation (hydrogel) containing silibinin-loaded pomegranate oil based nanocapsules suspension and its evaluation as an alternative for the treatment of cutaneous UVB radiation-induced damages. For this, an animal model of skin injury induced by UVB radiation was employed. Gellan gum was used as gel forming agent by its direct addition to nanocapsules suspension. The hydrogels showed adequate pH values (5.6-5.9) and a silibinin content close to the theoretical value (1mg/g). Through vertical Franz diffusion cells it was demonstrated that nanocapsules decreased the silibinin retention in the semisolid formulation. All formulations were effective in reducing mice ear edema and leukocyte infiltration induced by UVB radiation 24h after the treatments. After 48h, only the hydrogels containing nanocapsules or silibinin associated with pomegranate oil demonstrated anti-edematogenic effect, as well as the positive control (hydrogel containing silver sulfadiazine 1%). After 72h, the hydrogel containing unloaded pomegranate oil based nanocapsules still presented a small activity. In conclusion, the results of this investigation demonstrated the feasibility to prepare a semisolid formulation presenting performance comparable to the traditional therapeutic option for skin burns (silver sulfadiazine) and with prolonged in vivo anti-inflammatory activity compared to the non-nanoencapsulated compounds.
authors.affiliation: Laboratório de Tecnologia Farmacêutica, Programa de Pós-graduação em Ciências: Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000, Prédio 26 - Camobi, Santa Maria - RS, 97105-900 Santa Maria, Brazil.
authors.forename: Marila Crivellaro Lay
authors.initials: MCL
authors.lastname: Marchiori
authors.affiliation: Laboratório de Tecnologia Farmacêutica, Programa de Pós-graduação em Ciências: Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000, Prédio 26 - Camobi, Santa Maria - RS, 97105-900 Santa Maria, Brazil.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rigon
authors.affiliation: Laboratório de Neurotoxicidade e Psicofarmacologia, Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima, 1000, Prédio 18 - Camobi, Santa Maria - RS, 97105-900 Santa Maria, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Camponogara
authors.affiliation: Laboratório de Neurotoxicidade e Psicofarmacologia, Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima, 1000, Prédio 18 - Camobi, Santa Maria - RS, 97105-900 Santa Maria, Brazil.
authors.forename: Sara Marchesan
authors.initials: SM
authors.lastname: Oliveira
authors.affiliation: Laboratório de Tecnologia Farmacêutica, Programa de Pós-graduação em Ciências: Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000, Prédio 26 - Camobi, Santa Maria - RS, 97105-900 Santa Maria, Brazil. Electronic address: leticiacruz@smail.ufsm.br.
authors.forename: Letícia
authors.initials: L
authors.lastname: Cruz
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000627:therapeutic use; D004487:Edema / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D029561:Lythraceae / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry*; D012837:Silver Sulfadiazine / Q000627:therapeutic use; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry* / Q000627:therapeutic use; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000893:Anti-Inflammatory Agents; D053769:Nanocapsules; D010938:Plant Oils; D012838:Silymarin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D000077385:Silybin; D009195:Peroxidase; D012837:Silver Sulfadiazine
keywords: Nanoparticles; Photodamage; Semisolid formulation; Silymarin; UVB; Vegetable oil