title: Comparison of salivary hemoglobin measurements for periodontitis screening.
abstract: Salivary hemoglobin (Hb) for screening of periodontitis is approved under the pharmaceutical affairs law of Japan. Two reagents are commercially available for the modified fecal occult blood test: Saliva Hemo Plus and OC-AUTO S Latex Reagent. We simultaneously measured split specimens from 561 samples by using these two methods and compared the differences and agreement between both methods. Moreover, saliva samples were collected from 10 subjects at five time points during the day for analysis of circadian variations and fluctuation. The Pearson's correlation coefficient for these two reagents was 0.794. The Bland-Altman plot of differences in salivary Hb levels measured by the two reagents indicated that the difference included fixed errors (0.55 μg/mL). On analysis of circadian variations, no statistically significant differences were observed using the Friedman test. However, fixed errors were observed between wake-up time and before dinner and before lunch and before dinner, and no random errors were observed by Bland-Altman analysis. In conclusion, the salivary Hb levels measured using OC-AUTO S Latex Reagent were lower than those measured using Saliva Hemo Plus, along with a tendency for higher levels in the morning. Thus, when performing salivary tests these observations must be considered.
authors.affiliation: Department of Translational Research, School of Dental Medicine, Tsurumi University.
authors.forename: Ayako
authors.initials: A
authors.lastname: Okada
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nomura
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sogabe
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Oku
authors.forename: Arika
authors.initials: A
authors.lastname: Sato Gillbreath
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Hino
authors.forename: Hideo
authors.initials: H
authors.lastname: Hayashi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yoshino
authors.forename: Hisanori
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Suzuki
authors.forename: Keizo
authors.initials: K
authors.lastname: Koresawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Koba
authors.forename: Kimiyuki
authors.initials: K
authors.lastname: Uetani
authors.forename: Mami
authors.initials: M
authors.lastname: Kotoh
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Nishitsuji
authors.forename: Satoshi
authors.initials: S
authors.lastname: Akutsu
authors.forename: Takakazu
authors.initials: T
authors.lastname: Nakasone
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tobi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Fukuzawa
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Yabuki
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Naono
authors.forename: Masataka
authors.initials: M
authors.lastname: Yajima
authors.forename: Keita
authors.initials: K
authors.lastname: Shimizu
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanada
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000175:diagnosis*; D012463:Saliva / Q000378:metabolism*
chemical_list: D006454:Hemoglobins