title: A statistical approach for optimization of alkaline lipase production by ascidian associated-Halobacillus trueperiRSK CAS9.
abstract: A marine ascidian-associated bacterium,Halobacillus trueperiRSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0. An alkaline lipase was purified to 8.46 fold with 1193.59 U mg-1specific activities with the molecular weight of 44 kDa. The activity was substantially inhibited by EDTA and PMSF, indicating that it was a metalloenzyme serine residue which was essential for catalytic activity. Thus, lipase production by microbial conversion of marine fish wastes in this study suggested its potential utilization for the production of high value products.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Sathishkumar
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Gnanakkan
authors.initials: G
authors.lastname: Ananthan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Kathirvel
authors.initials: K
authors.lastname: Iyappan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Chinnathambi
authors.initials: C
authors.lastname: Stalin
keywords: Didemnum candidum; Haloalkaline lipase; Marine wastes; Response surface methodology