title: Establishing a small animal model for evaluating protective immunity against mumps virus.
abstract: Although mumps vaccines have been used for several decades, protective immune correlates have not been defined. Recently, mumps outbreaks have occurred in vaccinated populations. To better understand the causes of the outbreaks and to develop means to control outbreaks in mumps vaccine immunized populations, defining protective immune correlates will be critical. Unfortunately, no small animal model for assessing mumps immunity exists. In this study, we evaluated use of type I interferon (IFN) alpha/beta receptor knockout mice (IFN-α/βR-/-) for such a model. We found these mice to be susceptible to mumps virus administered intranasally and intracranially. Passive transfer of purified IgG from immunized mice protected naïve mice from mumps virus infection, confirming the role of antibody in protection and demonstrating the potential for this model to evaluate mumps immunity.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Adrian
authors.initials: A
authors.lastname: Pickar
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Pei
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Andrew
authors.initials: A
authors.lastname: Elson
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
authors.forename: James
authors.initials: J
authors.lastname: Zengel
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, United States of America.
authors.forename: Christian
authors.initials: C
authors.lastname: Sauder
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, United States of America.
authors.forename: Steve
authors.initials: S
authors.lastname: Rubin
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Biao
authors.identifier: http://orcid.org/0000-0001-8336-7171
authors.initials: B
authors.lastname: He
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000378:metabolism; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009107:Mumps / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control* / Q000821:virology*; D009109:Mumps virus / Q000276:immunology* / Q000472:pathogenicity*; D053633:Receptor, Interferon alpha-beta / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D053633:Receptor, Interferon alpha-beta