title: Proteomic response of Macrobrachium rosenbergii hepatopancreas exposed to chlordecone: Identification of endocrine disruption biomarkers?
abstract: The present work is the first study investigating the impacts of chlordecone, an organochlorine insecticide, on the proteome of the decapod crustacean Macrobrachium rosenbergii, by gel-free proteomic analysis. The hepatopancreas protein expression variations were analysed in organisms exposed to three environmental relevant concentrations of chlordecone (i.e. 0.2, 2 and 20µg/L). Results revealed that 62 proteins were significantly up- or down-regulated in exposed prawns compared to controls. Most of these proteins are involved in important physiological processes such as ion transport, defense mechanisms and immune system, cytoskeleton dynamics, or protein synthesis and degradation. Moreover, it appears that 6% of the deregulated protein are involved in the endocrine system and in the hormonal control of reproduction or development processes of M. rosenbergii (e.g. vitellogenin, farnesoic acid o-methyltransferase). These results indicate that chlordecone is potentially an endocrine disruptor compound for decapods, as already observed in vertebrates. These protein modifications could lead to disruptions of M. rosenbergii growth and reproduction, and therefore of the fitness population on the long-term. Besides, these disrupted proteins could be suggested as biomarkers of exposure for endocrine disruptions in invertebrates. However, further investigations are needed to complete understanding of action mechanisms of chlordecone on proteome and endocrine system of crustaceans.
authors.affiliation: University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE) - Freshwater and OceaniC sciences Unit of reSearch (FOCUS), Chemistry Institute, Bât. B6C, 11 allée du 6 Août, B-4000 Sart-Tilman, Belgium. Electronic address: Anne.Lafontaine@ulg.ac.be.
authors.forename: Anne
authors.initials: A
authors.lastname: Lafontaine
authors.affiliation: Laboratory of Mass Spectrometry, University of Liège, Liège, Belgium; GIGA Proteomics Facility, University of Liège, Liège, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Baiwir
authors.affiliation: University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE) - Freshwater and OceaniC sciences Unit of reSearch (FOCUS), Chemistry Institute, Bât. B6C, 11 allée du 6 Août, B-4000 Sart-Tilman, Belgium.
authors.forename: Célia
authors.initials: C
authors.lastname: Joaquim-Justo
authors.affiliation: Laboratory of Mass Spectrometry, University of Liège, Liège, Belgium; GIGA Proteomics Facility, University of Liège, Liège, Belgium.
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.affiliation: DYNECAR-UMR BOREA (MNHN/CNRS 7208/IRD207/UPMC/UA), University of the French West Indies, Campus de Fouillole, F-97110 Pointe-à-Pitre, Guadeloupe, France.
authors.forename: Soazig
authors.initials: S
authors.lastname: Lemoine
authors.affiliation: Normandie University, ULH, UMR I-02, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO) - FR CNRS 3730 SCALE, F-76600 Le Havre, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Boulangé-Lecomte
authors.affiliation: Normandie University, ULH, UMR I-02, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO) - FR CNRS 3730 SCALE, F-76600 Le Havre, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Forget-Leray
authors.affiliation: University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE) - Freshwater and OceaniC sciences Unit of reSearch (FOCUS), Chemistry Institute, Bât. B6C, 11 allée du 6 Août, B-4000 Sart-Tilman, Belgium.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Thomé
authors.affiliation: University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE) - Freshwater and OceaniC sciences Unit of reSearch (FOCUS), Chemistry Institute, Bât. B6C, 11 allée du 6 Août, B-4000 Sart-Tilman, Belgium.
authors.forename: Eric
authors.initials: E
authors.lastname: Gismondi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D007631:Chlordecone / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D043143:Hepatopancreas / Q000187:drug effects* / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D008780:Methyltransferases / Q000378:metabolism; D033541:Palaemonidae / Q000187:drug effects* / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D012098:Reproduction / Q000187:drug effects; D014819:Vitellogenins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D007306:Insecticides; D020543:Proteome; D014819:Vitellogenins; D008780:Methyltransferases; C032433:farnesoic acid O-methyltransferase; D007631:Chlordecone
keywords: Biomarkers; Chlordecone; Endocrine disruption; Invertebrates; Macrobrachium; Proteomics