title: Generation of the Acute Phencyclidine Rat Model for Proteomic Studies of Schizophrenia.
abstract: Administration of the non-competitive N-methyl-D-aspartate (NMDA) receptor antagonist phencyclidine (PCP) to rodents has been used as the gold standard preclinical model for schizophrenia. PCP treatment induces hyperlocomotion and stereotypic behaviour, which resemble the positive symptoms of schizophrenia. In addition, proteomic studies have identified changes in proteins associated with energy metabolism and neurotransmission which are typical hallmarks of the disease. This chapter presents a protocol for the generation of this model, behavioural assessment and preparation of key bio-samples to provide the raw materials for proteomic analyses.
authors.affiliation: Department of neurosciences, University of Cambridge, Clifford Allbutt building, Hills road, Cambridge, CB2 0AH, UK.
authors.forename: Dan
authors.initials: D
authors.lastname: Ma
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, Brazil. paulcguest@yahoo.com.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D000208:Acute Disease; D000818:Animals; D001923:Brain Chemistry; D004195:Disease Models, Animal; D006728:Hormones / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D007249:Inflammation; D008124:Locomotion; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010622:Phencyclidine / Q000633:toxicity*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012559:Schizophrenia / Q000139:chemically induced* / Q000378:metabolism; D044967:Serum
chemical_list: D006728:Hormones; D009419:Nerve Tissue Proteins; D010622:Phencyclidine
keywords: Behaviour; Brain tissue; PCP; Proteomics; Rat model; Schizophrenia; Serum