title: Exosome production and its regulation of EGFR during wound healing in renal tubular cells.
abstract: Kidney repair following injury involves the reconstitution of a structurally and functionally intact tubular epithelium. Growth factors and their receptors, such as EGFR, are important in the repair of renal tubules. Exosomes are cell-produced small (~100 nm in diameter) vesicles that contain and transfer proteins, lipids, RNAs, and DNAs between cells. In this study, we examined the relationship between exosome production and EGFR activation and the potential role of exosome in wound healing. EGFR activation occurred shortly after scratch wounding in renal tubular cells. Wound repair after scratching was significantly promoted by EGF and suppressed by EGFR inhibitor gefitinib. Interestingly, scratch wounding induced a significant increase of exosome production. The exosome production was decreased by EGF and increased by gefitinib, suggesting a suppressive role of EGFR signaling in exosome production. Conversely, inhibition of exosome release by GW4869 and manumycin A markedly increased EGFR activation and promoted wound healing. Moreover, exosomes derived from scratch-wounding cells could inhibit wound healing. Collectively, the results indicate that wound healing in renal tubular cells is associated with EGFR activation and exosome production. Although EGFR activation promotes wound healing, released exosomes may antagonize EGFR activation and wound healing.
authors.affiliation: Department of Urology, Taihe Hospital, Hubei University of Medicine, Hubei, China.|Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia.|Department of Nephrology, The Third Xiangya Hospital of Central South University, Changsha, China; and.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Urology, Taihe Hospital, Hubei University of Medicine, Hubei, China.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Yao
authors.affiliation: Department of Nephrology, The Third Xiangya Hospital of Central South University, Changsha, China; and.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia.
authors.forename: Guie
authors.initials: G
authors.lastname: Dong
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Chen
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia; zdong@augusta.edu.|Department of Nephrology, The Second Xiangya Hospital of Central South University, Changsha, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Dong
mesh_terms: D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D001597:Benzylidene Compounds / Q000494:pharmacology; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D055354:Exosomes / Q000378:metabolism* / Q000473:pathology; D000077156:Gefitinib; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D011090:Polyenes / Q000494:pharmacology; D053284:Polyunsaturated Alkamides / Q000494:pharmacology; D011799:Quinazolines / Q000494:pharmacology; D015398:Signal Transduction; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000814:Aniline Compounds; D001597:Benzylidene Compounds; C468773:GW 4869; D011090:Polyenes; D053284:Polyunsaturated Alkamides; D011799:Quinazolines; D004815:Epidermal Growth Factor; C541485:EGFR protein, mouse; D066246:ErbB Receptors; C054474:manumycin; D000077156:Gefitinib
keywords: EGFR; exosomes; kidney; wound healing