title: A Self-Quenching System Based on Bis-Naphthalimide: A Dual Two-Photon-Channel GSH Fluorescent Probe.
abstract: A single fluorescent signal output often does not satisfy the detection accuracy requirements for bioactive compounds in biological systems. It is of great interest to develop a dual-channel turn-on fluorescence sensing system for self-validated detection. Herein, we report a self-quenching nanoparticle composed of dual two-photon dyes of naphthalimides, displaying almost no fluorescence at both channels, owing to aggregation-caused quenching (ACQ). A dual two-photon-channel fluorescent probe for GSH was further developed by inserting a disulfide bond between two naphthalimides. Upon treatment with GSH, the disulfide bond was cleaved, and two napthalimide fluorophores were separated, leading to turn-on fluorescence at both channels. This dual-two-photon-channel design strategy can be easily extended to other analytes, simply by changing the linker molecules, which creates new opportunities to accurately monitor various biological processes in living cells.
authors.affiliation: Department of General Surgery, Jinhua Central Hospital, Jinhua, 321000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Ge
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, China.
authors.forename: Siyang
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.
authors.forename: Ruoyu
authors.identifier: http://orcid.org/0000-0002-4086-9566
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, China.
authors.forename: Chengyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Jinhua Central Hospital, Jinhua, 321000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Jinhua Central Hospital, Jinhua, 321000, China.
authors.forename: Shian
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D015394:Molecular Structure; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry*; D017785:Photons; D011789:Quantum Theory
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D005978:Glutathione
keywords: FRET; aggregation-caused quenching; fluorescent probes; naphthalimides; two-photon probes