title: PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.
abstract: A PCR-dipstick chromatography technique was designed and evaluated for differential identification ofblaNDM,blaKPC,blaIMP, andblaOXA-48carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
authors.affiliation: Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Rathina Kumar
authors.initials: RK
authors.lastname: Shanmugakani
authors.affiliation: Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan akeda@biken.osaka-u.ac.jp.|Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akeda
authors.affiliation: Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Sakamoto
authors.affiliation: Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Hagiya
authors.affiliation: Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.|Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Dan
authors.initials: D
authors.lastname: Takeuchi
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Yo
authors.initials: Y
authors.lastname: Sugawara
authors.affiliation: Tohoku Bio-Array, Sendai, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kodera
authors.affiliation: Tohoku Bio-Array, Sendai, Japan.|Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kawase
authors.affiliation: Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Warawut
authors.initials: W
authors.lastname: Laolerd
authors.affiliation: Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Narong
authors.initials: N
authors.lastname: Chaihongsa
authors.affiliation: Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Pitak
authors.initials: P
authors.lastname: Santanirand
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ishii
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Hamada
authors.affiliation: Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.|Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tomono
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Enterobacteriaceae; carbapenemase; molecular diagnostics