title: Structural variations of lignin macromolecule from different growth years of Triploid of Populus tomentosa Carr.
abstract: To better understand the variations of structural characteristics of lignin macromolecules during different growth years of Triploid of Populus tomentosa Carr, a novel lignin isolation procedure based on double ball-milling and enzymatic hydrolysis (DEL) was proposed in this study. The morphological distributions of lignin in the plant cell wall of these poplar wood samples were monitored by Confocal Raman Microscopy (CRM). The ultrahigh yields (105.1%-111.2%) of DELs were significantly higher than those (24.4-31.8%) of corresponding cellulolytic enzyme lignins (CELs). DELs and CELs were elaborately characterized by HPAEC, GPC, 2D-HSQC NMR and31P NMR techniques, and NMR results showed that DEL samples possess similar structural features as compared to CEL counterparts except for the decreased S/G ratio and p-hydroxybenzoate (PB) as well as increased p-hydroxyphenyl units (H). There are no obvious differences in the structural characteristics except for high contents of PB and H units in DEL-1, as well as high S/G ratio and β-O-4' linkages in DEL-5. It is believed that the DEL proposed in the present study can be used for characterizing the entire structural features of lignin macromolecules in the plant cell wall of different kinds of lignocellulosic biomass.
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Tian-Ying
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China. Electronic address: wenjialonghello@126.com.
authors.forename: Jia-Long
authors.initials: JL
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Chuan-Fu
authors.initials: CF
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Tong-Qi
authors.initials: TQ
authors.lastname: Yuan
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Run-Cang
authors.initials: RC
authors.lastname: Sun
mesh_terms: D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D008970:Molecular Weight; D032107:Populus / Q000737:chemistry* / Q000235:genetics* / Q000254:growth & development; D057885:Triploidy; D014934:Wood / Q000737:chemistry
chemical_list: D008031:Lignin
keywords: Lignin; Lignin distribution; NMR; Poplar wood; Structural characteristics