title: Systematic Trial for Evaluating Docetaxel in a Human Prostate Cancer Cell DU145 Xenograft Model.
abstract: The inhibitory activities of docetaxel at a wide range of doses (0.1-10 mg/kg; subcutaneously (s.c.), once/week) in nude mice bearing a human prostate cancer cell (DU145), xenograft model with implantation of DU145 cells or a DU145 solid tumor were examined. This systematic trial demonstrated that (i) docetaxel was more effective in the xenograft model formed by implantation of DU145 cells than in the solid DU145 tumor; (ii) administration of 2.5 mg/kg docetaxel was the critical dose because inhibitory activities were not observed at 2.5 mg/kg, while they were noted at 5 mg/kg and 10 mg/kg in both implantation approaches; (iii) edema-like effects (plump body shape and legs) were observed in both groups at 2.5 mg/kg and the tumor sizes, often increased by blood plasma and other fluids, as well as body weights were higher than at other doses; and (iv) suppression of body weight gain was observed at 10 mg/kg docetaxel.
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Mabuchi
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ueda
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Horiike
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Yamaoka
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Syuhei
authors.initials: S
authors.lastname: Nakao
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ueda
authors.affiliation: Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
authors.forename: Kazutake
authors.initials: K
authors.lastname: Tsujikawa
authors.affiliation: School of Pharmacy, Hyogo University of Health Sciences, Kobe, Japan tanaka-a@huhs.ac.jp.|Laboratory of Chemical Biology, Advanced Medicinal Research Center, Hyogo University of Health Sciences, Kobe, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077143:Docetaxel; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D043823:Taxoids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D043823:Taxoids; D000077143:Docetaxel
keywords: DU145; Docetaxel; anti-cancer drug; dose-dependency; nude mice; side-effects; xenograft model