title: Emerging roles of MICAL family proteins - from actin oxidation to membrane trafficking during cytokinesis.
abstract: Cytokinetic abscission is the terminal step of cell division, leading to the physical separation of the two daughter cells. The exact mechanism mediating the final scission of the intercellular bridge connecting the dividing cells is not fully understood, but requires the local constriction of endosomal sorting complex required for transport (ESCRT)-III-dependent helices, as well as remodelling of lipids and the cytoskeleton at the site of abscission. In particular, microtubules and actin filaments must be locally disassembled for successful abscission. However, the mechanism that actively removes actin during abscission is poorly understood. In this Commentary, we will focus on the latest findings regarding the emerging role of the MICAL family of oxidoreductases in F-actin disassembly and describe how Rab GTPases regulate their enzymatic activity. We will also discuss the recently reported role of MICAL1 in controlling F-actin clearance in the ESCRT-III-mediated step of cytokinetic abscission. In addition, we will highlight how two other members of the MICAL family (MICAL3 and MICAL-L1) contribute to cytokinesis by regulating membrane trafficking. Taken together, these findings establish the MICAL family as a key regulator of actin cytoskeleton dynamics and membrane trafficking during cell division.
authors.affiliation: Membrane Traffic and Cell Division Lab, Cell Biology and Infection department, Institut Pasteur, 25-28 rue du Dr Roux, Paris CEDEX 15 75724, France stephane.fremont@pasteur.fr arnaud.echard@pasteur.fr.|Centre National de la Recherche Scientifique UMR3691, Paris 75015, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Frémont
authors.affiliation: Institut Jacques Monod, CNRS, Université Paris Diderot, Université Sorbonne Paris Cité, Paris 75013, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Romet-Lemonne
authors.affiliation: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, Paris F-75005, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Houdusse
authors.affiliation: Membrane Traffic and Cell Division Lab, Cell Biology and Infection department, Institut Pasteur, 25-28 rue du Dr Roux, Paris CEDEX 15 75724, France stephane.fremont@pasteur.fr arnaud.echard@pasteur.fr.|Centre National de la Recherche Scientifique UMR3691, Paris 75015, France.
authors.forename: Arnaud
authors.identifier: 0000-0001-7402-1398
authors.initials: A
authors.lastname: Echard
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D048749:Cytokinesis; D006801:Humans; D005810:Multigene Family; D010084:Oxidation-Reduction; D021381:Protein Transport
chemical_list: D000199:Actins
keywords: Actin; Cytokinesis; MICAL; Membrane trafficking; Oxidoreduction; Rab GTPase