title: Digital Morphometrics of Two North American Grapevines (Vitis: Vitaceae) Quantifies Leaf Variation between Species, within Species, and among Individuals.
abstract: Recent studies have demonstrated that grapevine (Vitisspp.) leaf shape can be quantified using digital approaches which indicate phylogenetic signal in leaf shape, discernible patterns of developmental context within single leaves, and signatures of local environmental conditions. Here, we extend this work by quantifying intra-individual, intraspecific, and interspecific variation in leaf morphology in accessions of North AmericanVitis ripariaandV. rupestrisin a common environment. For each species at least four clonal replicates of multiple genotypes were grown in the Missouri Botanical Garden Kemper Center for Home Gardening. All leaves from a single shoot were harvested and scanned leaf images were used to conduct generalized Procrustes analysis, linear discriminant analysis, and elliptical Fourier analysis. Leaf shapes displayed genotype-specific signatures and species distinctions consistent with taxonomic classifications. Leaf shape variation within genotypes and among clones was the result of pest and pathogen-induced leaf damage that alters leaf morphology. Significant trends in leaf damage caused by disease and infestation were non-random with respect to leaf position on the shoot. Digital morphometrics is a powerful tool for assessing leaf shape variation among species, genotypes, and clones under common conditions and suggests biotic factors such as pests and pathogens as important drivers influencing leaf shape.
authors.affiliation: Department of Biology, Saint Louis University, St. LouisMO, USA; Science and Conservation Department, Missouri Botanical Garden, St. LouisMO, USA.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Klein
authors.affiliation: Department of Biology, Saint Louis University, St. Louis MO, USA.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Caito
authors.affiliation: Department of Biology, Saint Louis University, St. Louis MO, USA.
authors.forename: Chad
authors.initials: C
authors.lastname: Chapnick
authors.affiliation: Department of Biology, Saint Louis University, St. Louis MO, USA.
authors.forename: Cassandra
authors.initials: C
authors.lastname: Kitchen
authors.affiliation: Department of Biology, Saint Louis University, St. Louis MO, USA.
authors.forename: Regan
authors.initials: R
authors.lastname: O'Hanlon
authors.affiliation: Independent Researcher, St. Louis MO, USA.
authors.forename: Dan H
authors.initials: DH
authors.lastname: Chitwood
authors.affiliation: Department of Biology, Saint Louis University, St. LouisMO, USA; Science and Conservation Department, Missouri Botanical Garden, St. LouisMO, USA.
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Miller
keywords: Vitis; digital morphometrics; elliptical Fourier descriptors; generalized Procrustes analysis; leaf shape; linear discriminant analysis