title: High-throughput ultra high performance liquid chromatography combined with mass spectrometry approach for the rapid analysis and characterization of multiple constituents of the fruit of Acanthopanax senticosus (Rupr. et Maxim.) Harms.
abstract: Acanthopanax senticosus (Rupr. et Maxim.) Harms, a traditional Chinese medicine, has been widely used to improve the function of skeleton, heart, spleen and kidney. This fruit is rich in nutrients, but the chemical constituents of Acanthopanax senticosus fruit are still unclear. A rapid method based on ultra high performance liquid chromatography with time-of-flight mass spectrometry was developed for the compound analysis of Acanthopanax senticosus fruit in vitro and in vivo. In this study, the Acanthopanax senticosus fruit could significantly increase the weight of immune organs, promote the proliferation of lymphatic T cells, regulate the lymphatic B cell function, and decrease the ability of natural killer cells. A total of 104 compounds of Acanthopanax senticosus fruit including lignans, flavones, triterpenoidsaponins, phenolic acids, and other constituents were identified. Among them, seven chemical compounds were reported for the first time in the Acanthopanax senticosus fruit. Compared with the serum sample of blank and dosed samples, 24 prototype compositions were characterized. The results of our experiment could be helpful to understand the complex compounds of Acanthopanax senticosus fruit in vitro and in vivo for further pharmacological activity studies.
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Yingzhi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Xiangcai
authors.initials: X
authors.lastname: Meng
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Sino-America Chinmedomics Technology Collaboration Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau.
authors.forename: Xijun
authors.identifier: http://orcid.org/0000-0001-9375-8280
authors.initials: X
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D028001:Eleutherococcus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis; D017705:Lignans / Q000032:analysis; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis*; D012503:Saponins / Q000032:analysis; D014315:Triterpenes / Q000032:analysis
chemical_list: D062385:Hydroxybenzoates; D017705:Lignans; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C017616:phenolic acid
keywords: Acanthopanax senticosus fruit; immunomodulation; mass spectrometry; traditional Chinese medicine; ultra-high performance liquid chromatography