title: Integration of human papillomavirus type 16 in cervical cancer cells.
abstract: Cervical cancer remains an important cause of women morbidity and mortality. The progression of cervical pathology correlates with the HPV integration into the host genome. However, the data on the viral integration status in cervical dysplasias are controversial. The aim of the current study was to evaluate the status of HPV integration in two types of cervical pathology - invasive and non invasive cervical cancer (e.g. carcinomain situ). 156 women were included in the study: 66 women were diagnosed with invasive cervical cancer (CC) and 90 with non invasive cervical cancer (carcinomain situ, CIS). 74.2% [95% PI: 63.64÷84.76] of specimens collected from women with diagnosed CC and 85.6% [95% PI: 85.53÷92.85] of CIS specimens were positive for HPV. The most prevalent HPV genotype in both groups was HPV16. To evaluate HPV integration, three selected HPV16 E2 gene fragments were analyzed by PCR. In the majority of CC and CIS specimens the amplification of all three HPV16 E2 gene fragments was observed. The episomal HPV16 form was detected in the majority of CC and CIS specimens. The deletion of all three HPV16 E2 gene fragments was detected in 9.4% of CC specimens and 2.2% of CIS specimens. Finally, integration status could not be used as diagnostical additional test to distinguish between invasive and non invasive cervical cancer.
authors.affiliation: Institute of Oncology, Vilnius University, Santariskiu str. 1, LT-08660 Vilnius, Lithuania.
authors.forename: Zivile
authors.initials: Z
authors.lastname: Gudleviciene
authors.affiliation: Institute of Oncology, Vilnius University, Santariskiu str. 1, LT-08660 Vilnius, Lithuania.
authors.forename: Daiva
authors.initials: D
authors.lastname: Kanopiene
authors.affiliation: Institute of Oncology, Vilnius University, Santariskiu str. 1, LT-08660 Vilnius, Lithuania. Vilnius University, Universiteto str. 3, LT-01513 Vilnius, Lithuania.
authors.forename: Ausra
authors.initials: A
authors.lastname: Stumbryte
authors.affiliation: Vilnius University, Universiteto str. 3, LT-01513 Vilnius, Lithuania.
authors.forename: Raminta
authors.initials: R
authors.lastname: Bausyte
authors.affiliation: Vilnius University, Universiteto str. 3, LT-01513 Vilnius, Lithuania.
authors.forename: Edgaras
authors.initials: E
authors.lastname: Kirvelaitis
authors.affiliation: Institute of Biotechnology of Vilnius University, V.A.Graiciuno 8, LT-02241 Vilnius, Lithuania.
authors.forename: Vaida
authors.initials: V
authors.lastname: Simanaviciene
authors.affiliation: Institute of Biotechnology of Vilnius University, V.A.Graiciuno 8, LT-02241 Vilnius, Lithuania.
authors.forename: Aurelija
authors.initials: A
authors.lastname: Zvirbliene
keywords: HPV16 E2 deletion; HPV16 integration; carcinoma in situ; cervical cancer