title: Preparation of Biodiesel with Liquid Synergetic Lipases from Rapeseed Oil Deodorizer Distillate.
abstract: To reduce industrial production cost, cheap and easily available rapeseed oil deodorizer distillates were used as feedstock to prepare biodiesel in this study. As a result, liquid forms of Candida rugosa lipase and Rhizopus oryzae lipase (ROL) were functioned as new and effective catalysts with biodiesel yield of 92.63% for 30 h and 94.36% for 9 h, respectively. Furthermore, the synergetic effect between the two lipases was employed to enhance biodiesel yield with a result of 98.16% in 6 h under optimized conditions via response surface methodology. The obtained conversion rate surpassed both yields of the individual two lipases and markedly shortened the reaction time. The resultant optimal conditions were ROL ratio 0.84, water content 46 wt% (w/w), reaction temperature 34 °C, and reaction time 6 h.
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, China-EU Institute for Clean and Renewable Energy, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
authors.forename: Leping
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
authors.forename: Yaojia
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
authors.forename: Liangcheng
authors.initials: L
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China. yanyunjun@hust.edu.cn.
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D055162:Biocatalysis; D056804:Biofuels; D001709:Biotechnology; D002175:Candida / Q000201:enzymology; D004951:Esterification; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D009812:Odorants; D000074262:Rapeseed Oil / Q000737:chemistry*; D012233:Rhizopus / Q000201:enzymology; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D056804:Biofuels; D000074262:Rapeseed Oil; D014867:Water; D008049:Lipase
keywords: Biodiesel; Candida rugosa lipase; Liquid lipase; Rapeseed oil deodorizer distillates (RODDs); Rhizopus oryzae lipase; Synergetic effect