title: Improving anaerobic digestion with support media: Mitigation of ammonia inhibition and effect on microbial communities.
abstract: This study aimed at providing a better understanding of the influence of support media (10g/L of zeolites, 10g/L of activated carbons, and 1g/L of chitosan) on key phylotypes steering anaerobic digestion (AD) performance in presence of 19g/L of Total Ammonia Nitrogen (TAN) within batch digesters. Support media did not influence TAN concentration. However, both zeolites and activated carbon 1 reduced methanization lag phase by 47% and 25%, respectively. By contrast, activated carbon 2 and chitosan led to an increase of methanization lag phase by 51% and 32%, respectively. 16S rRNA gene sequencing revealed that zeolites preserved Methanosarcina and enhanced Methanobacterium. In presence of activated carbon 1, Methanoculleus, became predominant earlier than without support while chitosan and activated carbon 2 limited its implantation. This study highlighted potentialities to use supports to enhance AD stability under extreme TAN concentration and evidenced their specific influence on the microbiota composition.
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: simon.poirier@irstea.fr.
authors.forename: Simon
authors.initials: S
authors.lastname: Poirier
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: celine.madigou@irstea.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Madigou
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: theodore.bouchez@irstea.fr.
authors.forename: Théodore
authors.initials: T
authors.lastname: Bouchez
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: olivier.chapleur@irstea.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Chapleur
mesh_terms: D000641:Ammonia / Q000494:pharmacology*; D000693:Anaerobiosis; D019638:Methanomicrobiaceae / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017641:Zeolites
chemical_list: D012336:RNA, Ribosomal, 16S; D017641:Zeolites; D000641:Ammonia
keywords: 16S rRNA gene sequencing; Activated carbon; Chitosan; Methanization; Total Ammonia Nitrogen; Zeolite