title: Gene structure of the pregnancy-associated glycoprotein-like (PAG-L) in the Eurasian beaver (Castor fiber L.).
abstract: The pregnancy-associated glycoprotein-like family (PAG-L) is a large group of chorionic products, expressed in the pre-placental trophoblast and later in the post-implantational chorionic epithelium, and are involved in proper placenta development and embryo-maternal interaction in eutherians. This study describes identification of the PAG-L family in the genome of the Eurasian beaver (Castor fiber L.), named CfPAG-L. We identified 7657 bp of the CfPAG-L gDNA sequence (Acc. No. KX377932), encompassing nine exons (1-9) and eight introns (A-H). The length of the CfPAG-L exons (59-200 bp) was equivalently similar to the only known counterparts of bPAG1, bPAG2, and pPAG2. The length of the CfPAG-L introns ranged 288-1937 bp and was completely different from previously known PAG introns. The exonic CfPAG-L regions revealed 50.3-72.9% homology with equivalent segments of bPAG1 and pPAG2 structure. The intronic CfPAG-L regions alignments revealed a lack of homology. Within the entire CfPAG-L gene, 31 potential single nucleotide variants (SNV: 7 transversions and 24 transitions) were predicted. The identified exonic polymorphic loci did not affect the amino acid sequence of the CfPAG-L polypeptide precursor. This is the first report describing the CfPAG-L gene sequence, structural organization, and SNVs in the Eurasian beaver, one of the largest rodents.
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland. aleksandra.lipka@uwm.edu.pl.|Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Niepodleglosci Str 44, 10-045, Olsztyn, Poland. aleksandra.lipka@uwm.edu.pl.
authors.forename: Aleksandra
authors.identifier: http://orcid.org/0000-0003-0815-6635
authors.initials: A
authors.lastname: Lipka
authors.affiliation: Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Warszawska Str 30, 10-082, Olsztyn, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Majewska
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Panasiewicz
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Bieniek-Kobuszewska
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Szafranska
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D007438:Introns; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011257:Pregnancy Proteins / Q000737:chemistry / Q000235:genetics*; D012377:Rodentia / Q000235:genetics*; D017385:Sequence Homology
chemical_list: D006023:Glycoproteins; D011257:Pregnancy Proteins; D016282:Aspartic Acid Endopeptidases
keywords: APs; Aspartic proteinases; Beaver; Exon-intron structure; PAGs; SNVs