title: LC-ESI-MS/MS profiling of phenolics in the leaves of Eleutherococcus senticosus cultivated in the West Europe and anti-hyaluronidase and anti-acetylcholinestarase activities.
abstract: Neither secondary metabolites of the spring leaves nor the autumn leaves of Eleutherococcus senticosus species cultivated in Poland, or the bioactivity are known. The richest in polyphenols was the autumn leaves (171.1 mg/g DE), while in flavonoids the spring leaves (107.9 mg/g DE). Using LC-ESI-MS/MS, protocatechuic acid has been identified as the most abundant compound in the spring and autumn leaves (200 and 70 μg/g DE, respectively). Amongst flavonoids, naringenin 7-O-glucoside occurred in the largest amount (20 and 10 mg/g DE in the spring and autumn leaves, respectively). The autumn leaves inhibited Hyal the strongest (74.3%), comparing to the spring leaves (33%). A weak inhibition was found towards AChE (0.64 and 5.8% for the autumn and spring leaves, respectively). To our best knowledge, no information was available on the phytochemical composition and activity of the leaves of E. senticosus cultivated in Poland.
authors.affiliation: a Department of Pharmacognosy, Ludwik Rydygier Collegium Medicum , Nicolaus Copernicus University , Bydgoszcz , Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Kuźniewski
authors.affiliation: a Department of Pharmacognosy, Ludwik Rydygier Collegium Medicum , Nicolaus Copernicus University , Bydgoszcz , Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Załuski
authors.affiliation: b Department of Pharmaceutical Botany , Medical University of Lublin , Lublin , Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Olech
authors.affiliation: c Rogów Arboretum , Forest Experimental Station, Warsaw University of Life Sciences , Rogów , Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Banaszczak
authors.affiliation: b Department of Pharmaceutical Botany , Medical University of Lublin , Lublin , Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Nowak
mesh_terms: D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002853:Chromatography, Liquid; D004353:Drug Evaluation, Preclinical / Q000379:methods; D028001:Eleutherococcus / Q000737:chemistry* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005060:Europe; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D005960:Glucosides / Q000032:analysis; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D064209:Phytochemicals / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011044:Poland; D059808:Polyphenols / Q000032:analysis*; D064210:Secondary Metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D064209:Phytochemicals; D010936:Plant Extracts; D059808:Polyphenols; C529068:naringenin-7-O-glucoside; D006821:Hyaluronoglucosaminidase
keywords: AChE; Eleutherococcus; Hyal; LC-ESI-MS/MS; flavonoids; phenolic acids