title: [Effect of nucleolin on cardiac cell apoptosis in Type 2 diabetic cardiomyopathy mice].
abstract: To investigate the effect of nucleolin on cardiac cell apoptosis in Type 2 diabetic cardiomyopathy mice.  Methods: Mice were fed with high-fat and high-sugar food for 20 weeks (mice were injected intraperitoneally with 60 mg/kg streptozotocin in the 5th and 6th weeks) to establish a mouse model of Type 2 diabetes. The mice were divided into 4 groups: a wild type (WT) control group, a nucleolin transgenic (TG) control group, a WT diabetic group, a TG diabetic group. Diabetes-related indicators were detected at the end of the 8th week. At the end of the 20th week, HE staining was used to observe myocardial morphological changes; TUNEL staining and caspase-3 activity were used to detect the extent of apoptosis of cardiac myocytes.  Results: The level of fasting blood glucose was significantly increased in the diabetic group than that in the control group. In WT diabetic group, myocardial disarrangement, fragmentation and dissolution were observed (determined by HE staining); cellular apoptosis (determined by TUNEL staining and caspase-3 activity) also increased markedly in the WT diabetic group. Compared with the wild mice in the diabetic group, myocardial morphological changes and cardiac myocytes apoptosis were alleviated significantly.   Conclusion: Nucleolin overexpression affectes the occurrence and development of diabetic cardiomyopathy through inhibition of cardiac myocyte apoptosis.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Meidong
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Bimei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Sipin
authors.initials: S
authors.lastname: Tan
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017209:Apoptosis; D001786:Blood Glucose / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000150:complications* / Q000378:metabolism; D058065:Diabetic Cardiomyopathies / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D005215:Fasting / Q000097:blood; D020287:In Situ Nick-End Labeling; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000473:pathology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013311:Streptozocin; D000097796:Nucleolin
chemical_list: D001786:Blood Glucose; D053148:Caspase 3; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D013311:Streptozocin; D000097796:Nucleolin