title: Monitoring the Disassembly of Virus-like Particles by19F-NMR.
abstract: Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of19F-labeled VLPs derived from bacteriophage Qβ by19F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems.
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Rogen L C
authors.initials: RLC
authors.lastname: Leung
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Matthew D M
authors.initials: MDM
authors.lastname: Robinson
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Alaa A A
authors.initials: AAA
authors.lastname: Ajabali
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Gogulan
authors.initials: G
authors.lastname: Karunanithy
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Brian
authors.initials: B
authors.lastname: Lyons
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Ritu
authors.initials: R
authors.lastname: Raj
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Saeed
authors.initials: S
authors.lastname: Raoufmoghaddam
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Shabaz
authors.identifier: 0000-0003-2640-9560
authors.initials: S
authors.lastname: Mohammed
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Timothy D W
authors.identifier: 0000-0001-5583-6460
authors.initials: TDW
authors.lastname: Claridge
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Baldwin
authors.affiliation: Chemistry Research Laboratory, Department of Chemistry, University of Oxford , Mansfield Road, Oxford OX1 3TA, United Kingdom.
authors.forename: Benjamin G
authors.identifier: 0000-0002-5056-407X
authors.initials: BG
authors.lastname: Davis
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry; D005461:Fluorine / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D058425:Vaccines, Virus-Like Particle / Q000032:analysis*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: C066119:Q protein, Bacteriophage lambda; D058425:Vaccines, Virus-Like Particle; D014764:Viral Proteins; D005461:Fluorine