title: Assessment of Olea europaea L. fruit extracts: Phytochemical characterization and anticancer pathway investigation.
abstract: Olea europaea L. has been widely used as an advantageous rich source of bioactive compounds of high economic value leading to its use in pharmaceutical, cosmetic, and agriculture industries. Ethanolic extracts of olive fruits from three different cultivars (OFE) were studied for their phytochemical contents and were investigated for antioxidant activities and anticancer potential. Major polyphenols detected in these extracts were tyrosol, hydroxytyrosol, oleuropein, rutin, quercetin and glucoside forms of luteolin and apigenin. All these compounds have shown to significantly contribute to the antioxidant activity of OFE, which was evaluated by DPPH and ABTS assays. Proliferation of hepatic and colon cancer cells, HepG2 and Caco-2, were shown to be sensitive to OFE with IC50less than 1.6mg/ml for all tested extracts. Moreover, flow cytometry analysis showed that OFE induced cell cycle arrest in the S-phase within both HepG2 and Caco-2 cells. This has triggered a cell death mechanism as shown by DNA fragmentation, expression of p53 and phosphorylation level of Akt and Erk proteins. Interestingly, these extracts could be further used as a potential source of natural compounds with both antioxidant and anticancer effects.
authors.affiliation: Environmental Bioprocesses Laboratory, Sfax Biotechnology Center, P.O. Box 1177, Sfax 3038, Tunisia.
authors.forename: Amina
authors.initials: A
authors.lastname: Maalej
authors.affiliation: Environmental Bioprocesses Laboratory, Sfax Biotechnology Center, P.O. Box 1177, Sfax 3038, Tunisia.
authors.forename: Zouhaier
authors.initials: Z
authors.lastname: Bouallagui
authors.affiliation: Environmental Bioprocesses Laboratory, Sfax Biotechnology Center, P.O. Box 1177, Sfax 3038, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Hadrich
authors.affiliation: Alliance for Research on North Africa (ARENA), University of Tsukuba, Tennodai 1-1-1, Tsukuba City, Ibaraki 305-8587, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba City 305-8587, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
authors.affiliation: Environmental Bioprocesses Laboratory, Sfax Biotechnology Center, P.O. Box 1177, Sfax 3038, Tunisia. Electronic address: sami.sayadi@cbs.rnrt.tn.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D031658:Olea / Q000737:chemistry*; D064209:Phytochemicals / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D016196:S Phase / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D059808:Polyphenols
keywords: Anticancer effect; Antioxidant effect; Caco-2 cells; HepG2 cells; Olive fruit extracts; Polyphenols