title: Phylogenomic inferences from reference-mapped and de novo assembled short-read sequence data using RADseq sequencing of California white oaks (Quercus section Quercus).
abstract: The emergence of next generation sequencing has increased by several orders of magnitude the amount of data available for phylogenetics. Reduced representation approaches, such as restriction-sited associated DNA sequencing (RADseq), have proven useful for phylogenetic studies of non-model species at a wide range of phylogenetic depths. However, analysis of these datasets is not uniform and we know little about the potential benefits and drawbacks of de novo assembly versus assembly by mapping to a reference genome. Using RADseq data for 83 oak samples representing 16 taxa, we identified variants via three pipelines: mapping sequence reads to a recently published draft genome of Quercus lobata, and de novo assembly under two sets of locus filters. For each pipeline, we inferred the maximum likelihood phylogeny. All pipelines produced similar trees, with minor shifts in relationships within well-supported clades, despite the fact that they yielded different numbers of loci (68 000 - 111 000 loci) and different degrees of overlap with the reference genome. We conclude that both the reference-aligned and de novo assembly pipelines yield reliable results, and that advantages and disadvantages of these approaches pertain mainly to downstream uses of RADseq data, not to phylogenetic inference per se.
authors.affiliation: a Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
authors.forename: Sorel
authors.initials: S
authors.lastname: Fitz-Gibbon
authors.affiliation: b The Morton Arboretum, 4100 Illinois Route 53, Lisle, IL 60532-1293, USA.|c The Field Museum, 1400 S Lake Shore Drive, Chicago, IL 60605, USA.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Hipp
authors.affiliation: b The Morton Arboretum, 4100 Illinois Route 53, Lisle, IL 60532-1293, USA.|d Department of Plant Biology, Michigan State University, East Lansing, MI 48824-1312, USA.
authors.forename: Kasey K
authors.initials: KK
authors.lastname: Pham
authors.affiliation: e Department of Biology, Duke University, Durham, NC 27708, USA.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Manos
authors.affiliation: f Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095-7239, USA.|g Institute of the Environment and Sustainability, University of California, Los Angeles, CA 90095-1496, USA.
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Sork
mesh_terms: D002140:California; D018744:DNA, Plant; D014644:Genetic Variation; D010802:Phylogeny; D029963:Quercus / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant
keywords: GATK; PyRAD; de novo clustering; groupement de novo; identification de variants; phylogenetics; phylogénétique; restriction-site associated DNA sequencing; séquençage de l’ADN associé aux sites de restriction; variant discovery