title: Fowl Adenovirus-Based Vaccine Platform.
abstract: Nonpathogenic fowl adenoviruses (FAdVs) are amenable for engineering multivalent vaccine platforms due to large stretches of nonessential DNA sequences in their genomes. We describe the generation of FAdV-9-based vaccine platforms by targeted homologous recombination in an infectious clone (pPacFAdV-9 or wild type FAdmid) containing the entire viral genome in a cosmid vector. The viral DNA is subsequently released from the cosmid by restriction enzyme digestion followed by transfection in a chicken hepatoma cell line (CH-SAH). Virus is harvested, propagated, and verified for foreign gene expression.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, Canada, N1G 2W1.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Corredor
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, Canada, N1G 2W1.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Pei
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, Canada, N1G 2W1. enagy@uoguelph.ca.
authors.forename: Éva
authors.initials: É
authors.lastname: Nagy
mesh_terms: D000818:Animals; D000259:Aviadenovirus / Q000235:genetics*; D045744:Cell Line, Tumor; D002645:Chickens; D003360:Cosmids / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D016679:Genome, Viral; D014162:Transfection; D014765:Viral Vaccines / Q000235:genetics*
chemical_list: D014765:Viral Vaccines
keywords: FAdV-9; FAdmid; Fowl adenovirus-9; Homologous recombination; Lambda red recombinase; Recombinant virus vaccine; Virus vector