title: PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186.
abstract: Vigorous angiogenesis is one of the reasons for the poor prognosis of glioma. A number of studies have shown that long non-coding RNA can affect a variety of biological behaviors of tumors. However, the influence of long non-coding RNAs on glioma vascular endothelial cells remains unclear. To simulate the glioma microenvironment, we applied glioma-conditioned medium to human cerebral microvascular endothelial cells. The long non-coding RNA PVT1 was found to be highly expressed in glioma vascular endothelial cells. Cell Counting Kit-8, migration, and tube formation assays showed that PVT1 overexpression promoted glioma vascular endothelial cells proliferation, migration, and angiogenesis. We also found that PVT1 overexpression upregulated the expression of the autophagy-related proteins Atg7 and Beclin1, which induced protective autophagy. Bioinformatics software and dual-luciferase system analysis confirmed that PVT1 acts by targeting miR-186. In addition, our study showed that miR-186 could target the 3' untranslated region of Atg7 and Beclin1 to decrease their expression levels, thereby inhibiting glioma-conditioned human cerebral microvascular endothelial cell autophagy. In conclusion, PVT1 overexpression increased the expression of Atg7 and Beclin1 by targeting miR-186, which induced protective autophagy, thus promoting glioma vascular endothelial cell proliferation, migration, and angiogenesis. Therefore, PVT1 and miR-186 can provide new therapeutic targets for future anti-angiogenic treatment of glioma.
authors.affiliation: 1 Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.|2 Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People's Republic of China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Ma
authors.affiliation: 3 Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, People's Republic of China.|4 Institute of Pathology and Pathophysiology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: 3 Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, People's Republic of China.|4 Institute of Pathology and Pathophysiology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Yixue
authors.initials: Y
authors.lastname: Xue
authors.affiliation: 1 Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.|2 Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People's Republic of China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Qu
authors.affiliation: 1 Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.|2 Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: 1 Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.|2 Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People's Republic of China.
authors.forename: Xiaobai
authors.initials: X
authors.lastname: Liu
authors.affiliation: 3 Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, People's Republic of China.|4 Institute of Pathology and Pathophysiology, China Medical University, Shenyang, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: 1 Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.|2 Liaoning Research Center for Translational Medicine in Nervous System Disease, Shenyang, People's Republic of China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001343:Autophagy / Q000235:genetics; D000071193:Autophagy-Related Protein 7 / Q000096:biosynthesis*; D000071186:Beclin-1 / Q000096:biosynthesis* / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D042783:Endothelial Cells / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000235:genetics; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D000071186:Beclin-1; C533096:MIRN186 microRNA, human; D035683:MicroRNAs; C521123:PVT1 long-non-coding RNA, human; D062085:RNA, Long Noncoding; C509349:ATG7 protein, human; D000071193:Autophagy-Related Protein 7
keywords: Glioma; PVT1; angiogenesis; endothelial cells; miR-186