title: Deferoxamine-conjugated AgInS2nanoparticles as new nanodrug for synergistic therapy for hepatocellular carcinoma.
abstract: Herein, a new nanodrug that exhibits multi-therapeutic modalities for synergistic treatment of hepatocellular carcinoma is reported. The nanodrug is composed of carboxymethyl cellulose modified silver indium sulfide nanoparticle (CMC-AgInS2NP, served as a source of reactive oxygen species) covalently linked with deferoxamine (DFO, served as iron chelating agent). The DFO/CMC-AgInS2nanodrug was taken up by the HepG2 cell and accumulated within the cytosol as well as the nucleus, leading to induction of cell arrest in the G2/M phase and subsequent apoptosis cell death. Compared to DFO, the DFO/CMC-AgInS2nanodrug demonstrated better anti-proliferative activity against the HepG2 cell. As they were cultured in a medium supplemented with ferric ions, the HepG2 cells were induced to grow faster as compared to the cells without the addition of ferric ions. Fortunately, our nanodrug was found to inhibit the cell growth induced by ferric ions. Our results indicate that the nanodrug has synergistic effect for treatment of HepG2 cells via the intrinsic therapeutic property of CMC-AgInS2NP and the iron chelating capability of DFO.
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Molecular Imaging (CEMI), Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; The Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Isara
authors.initials: I
authors.lastname: Phiwchai
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: ttpthongtem@yahoo.com.
authors.forename: Titipun
authors.initials: T
authors.lastname: Thongtem
authors.affiliation: Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Thongtem
authors.affiliation: Center of Excellence for Molecular Imaging (CEMI), Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: chalermchai.pilapong@cmu.ac.th.
authors.forename: Chalermchai
authors.initials: C
authors.lastname: Pilapong
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D003676:Deferoxamine / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000188:drug therapy*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D007502:Iron Chelating Agents; D003676:Deferoxamine
keywords: AgInS(2) nanoparticle; Deferoxamine; Iron chelator; Liver cancer; Synergistic therapy