title: New and Old Genes Associated with Topotecan Resistance Development in Ovarian Cancer Cell Lines.
abstract: Low effectiveness of chemotherapy in ovarian cancer results from development of drug resistance. Topotecan is a drug used as second-line chemotherapy for this cancer type. We analyzed development of topotecan resistance in ovarian cancer cell lines.
  
  
  
  A chemosensitivity assay, MTT test, was performed to assess drug resistance. Quantitative polymerase chain reaction (Q-PCR) assays were performed to determine ABCB1, ABCG2, ALDH1A1, IFIH1, SAMD4 and EPHA3 gene expression.
  
  
  
  We observed dose-dependent responses to topotecan. In all topotecan-resistant cell lines an overexpression of ABCG2, IFIH1 and SAMD4 genes was observed. Expression of ABCB1 gene was observed in one cell line. Expression of ALDH1A1 was up-regulated in A2780 and down-regulated in SKOV-3-resistant cell lines. Short-time exposure led to similar patterns of gene expression for the investigated genes.
  
  
  
  Expression of ABCG2 and ABCB1 genes plays the most important role in topotecan resistance. The role of other investigated genes seems to be complementary.
authors.affiliation: Department of Nursing, Poznan University of Medical Sciences, Poznan, Poland aklejewski@ump.edu.pl.|Department of Obstetrics and Womens Dieseases, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Klejewski
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Świerczewska
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zaorska
authors.affiliation: Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Brązert
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.|Department of Histology and Embryology, Wrocław Medical University, Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Januchowski
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D059004:Topoisomerase I Inhibitors / Q000494:pharmacology; D019772:Topotecan / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D018528:ATP-Binding Cassette Transporters; D014408:Biomarkers, Tumor; D059004:Topoisomerase I Inhibitors; D019772:Topotecan
keywords: Ovarian cancer; drug transporters; new genes; topotecan resistance