title: Production and characterization of a new antibacterial peptide obtained fromAeribacillus pallidusSAT4.
abstract: A novel thermophilic bacterial strain of the genusAeribacilluswas isolated from Thar Dessert Pakistan. This strain showed significant antibacterial activity againstMicrococcus luteus,Staphylococcus aureus,and Pseudomonasaeruginosa. The strain coded as 'SAT4' resembled withAeribacillus pallidusin the morphological, biochemical and molecular tests. The production of antibacterial metabolites by SAT4 was optimized. These active metabolites were precipitated by 50% ammonium sulphate and purified through sephadex G-75 gel permeation chromatography and reverse phase HPLC. The molecular weight of 37 kDa was examined by SDS-PAGE. The structural elucidation of the purified product was studied by FTIR,1H and13C NMR. The X-ray diffractions study showed that the crystals belonged to the primitive orthorhombic lattice (a= 12.137,b= 13.421,c= 14.097 Å) and 3D structure (proposed name: Aeritracin) was determined. This new peptide antibacterial molecule can get a position in pharmaceutical and biotechnological industrial research.
authors.affiliation: Department of Biotechnology, Quaid-I-Azam University, Islamabad 45320, Pakistan.
authors.forename: Syed Aun
authors.initials: SA
authors.lastname: Muhammad
authors.affiliation: Department of Microbiology, Quaid-I-Azam University, Islamabad 45320, Pakistan.
authors.forename: Safia
authors.initials: S
authors.lastname: Ahmed
keywords: Aeribacillus pallidus SAT4; Antibiotics; Characterization; Production