title: Isolation and characterization of the TaSnRK2.10 gene and its association with agronomic traits in wheat (Triticum aestivum L.).
abstract: Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s. Based on the haplotypes of TaSnRK2.10-4A (Hap-4A-H and Hap-4A-L), a cleaved amplified polymorphic sequence (CAPS) marker designated TaSnRK2.10-4A-CAPS was developed and mapped between the markers D-1092101 and D-100014232 using a set of recombinant inbred lines (RILs). The TaSnRK2.10-4B alleles (Hap-4B-G and Hap-4B-A) were transformed into allele-specific PCR (AS-PCR) markers TaSnRK2.10-4B-AS1 and TaSnRK2.10-4B-AS2, which were located between the markers D-1281577 and S-1862758. No diversity was found for TaSnRK2.10-4D. An association analysis using a natural population consisting of 128 winter wheat varieties in multiple environments showed that the thousand grain weight (TGW) and spike length (SL) of Hap-4A-H were significantly higher than those of Hap-4A-L, but pant height (PH) was significantly lower.
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Zhao-Gui
authors.initials: ZG
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.|Tai'an Academy of Agricultural Science, Tai'an, China.
authors.forename: Guang-de
authors.initials: GD
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Fan-Mei
authors.initials: FM
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Si-Shen
authors.identifier: http://orcid.org/0000-0002-3790-1617
authors.initials: SS
authors.lastname: Li
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D018556:Crops, Agricultural / Q000235:genetics* / Q000254:growth & development; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D017343:Genes, Plant; D056726:Genetic Association Studies; D006239:Haplotypes; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D011494:Protein Kinases