title: Cytokine and antioxidant gene profiles from peripheral blood mononuclear cells of Pelibuey lambs after Haemonchus contortus infection.
abstract: The expression profiles of cytokines and antioxidant genes were determined from an experimental infection with H. contortus in Pelibuey lambs. The infection was followed for 34 days (d) to determine the number of eggs per gram (epg) and the packed cell volume (PCV). Differential white cell counts and expression profile estimations of IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, FCεR1A, GPX and SOD1 were determined at 0 hour, 4 hours, 2 days and 14 days post-infection (PI) in infected and control groups. Comparison of the fold change between 0 and 4-hours, 4-hours and 2-days and 2- and 14-days periods was performed. Significant differences (P<.05) between epg (>2000) and PCV (>30%) were determined after 21 days and were also observed with regard to monocyte and lymphocyte cells after 2 and 7 days PI. At 0 hour and 14 days PI, the GPX and IL-2 genes showed a 0.37- and 0.49-fold decrease in expression, respectively. In contrast, upregulation was observed at 4 hours of IL-8 (2.58) and FCεR1A (2.71), at 2 days for IL-4 (2.14) and IL-8 (4.02) and at 14 days for IL-2 (0.41), IL-10 (2.35) and FCεR1A (2.28). The comparison between the intervals of infection showed high expression values against H. contortus infection in Pelibuey sheep after the 2nd period of PI involving a dichotomy T cells.
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Mérida-Xmatkuil, Yucatán, México.
authors.forename: Z
authors.initials: Z
authors.lastname: Estrada-Reyes
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Jiutepec, Morelos, México.
authors.forename: M E
authors.identifier: http://orcid.org/0000-0002-8688-4411
authors.initials: ME
authors.lastname: López-Arellano
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Mérida-Xmatkuil, Yucatán, México.
authors.forename: F
authors.initials: F
authors.lastname: Torres-Acosta
authors.affiliation: Instituto Nacional de Rehabilitación, Tlalpan, Ciudad de México, México.
authors.forename: A
authors.initials: A
authors.lastname: López-Reyes
authors.affiliation: Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México.
authors.forename: A
authors.initials: A
authors.lastname: Lagunas-Martínez
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Jiutepec, Morelos, México.
authors.forename: P
authors.initials: P
authors.lastname: Mendoza-de-Gives
authors.affiliation: Unidad Regional Universitaria Sur-Sureste, Universidad Autónoma de Chapingo, Tabasco, México.
authors.forename: R
authors.initials: R
authors.lastname: González-Garduño
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Jiutepec, Morelos, México.
authors.forename: S
authors.initials: S
authors.lastname: Olazarán-Jenkins
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Jiutepec, Morelos, México.
authors.forename: D
authors.initials: D
authors.lastname: Reyes-Guerrero
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Jiutepec, Morelos, México.
authors.forename: G
authors.initials: G
authors.lastname: Ramírez-Vargas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics*; D005243:Feces; D006188:Haemonchiasis / Q000276:immunology / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D010270:Parasite Egg Count; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D016207:Cytokines
keywords: 
  Haemonchus contortus
  ; Cytokines; expression profiling; immunoregulation; peripheral blood mononuclear cells; ruminant