title: The antimicrobial volatile power of the rhizospheric isolate Pseudomonas donghuensis P482.
abstract: Soil and rhizosphere bacteria produce an array of secondary metabolites including a wide range of volatile organic compounds (VOCs). These compounds play an important role in the long-distance interactions and communication between (micro)organisms. Furthermore, bacterial VOCs are involved in plant pathogens inhibition and induction of soil fungistasis and suppressivenes. In the present study, we analysed the volatile blend emitted by the rhizospheric isolate Pseudomonas donghuensis P482 and evaluated the volatile effect on the plant pathogenic fungi and bacteria as well as one oomycete. Moreover, we investigated the role of the GacS/GacA system on VOCs production in P. donghuensis P482. The results obtained demonstrated that VOCs emitted by P. donghuensis P482 have strong antifungal and antioomycete, but not antibacterial activity. The production of certain volatiles such as dimethyl sulfide, S-methyl thioacetate, methyl thiocyanate, dimethyl trisulfide, 1-undecan and HCN is depended on the GacS/GacA two-component regulatory system. Apparently, these compounds play an important role in the pathogens suppression as the gacA mutant entirely lost the ability to inhibit via volatiles the growth of tested plant pathogens.
authors.affiliation: Laboratory of Biological Plant Protection, Department of Biotechnology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Gdansk, Poland.|Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands.
authors.forename: Adam
authors.initials: A
authors.lastname: Ossowicki
authors.affiliation: Laboratory of Biological Plant Protection, Department of Biotechnology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Jafra
authors.affiliation: Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands.
authors.forename: Paolina
authors.identifier: http://orcid.org/0000-0002-2359-5719
authors.initials: P
authors.lastname: Garbeva
mesh_terms: D060054:Agrobacterium / Q000187:drug effects; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D005658:Fungi / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006856:Hydrogen Cyanide / Q000378:metabolism; D009868:Oomycetes / Q000187:drug effects; D011549:Pseudomonas / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000187:drug effects; D011775:Pythium / Q000187:drug effects; D012232:Rhizoctonia / Q000187:drug effects; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents; D001426:Bacterial Proteins; D055549:Volatile Organic Compounds; D006856:Hydrogen Cyanide