title: Effect of local hIL-10 gene therapy on experimental periodontitis in ovariectomized rats.
abstract: OBJECTIVE
  To investigate the effect of local hIL-10 gene therapy on experimental periodontitis in rats and to elucidate the mechanism underlying this effect.
  
  
  METHODS
  Experimental periodontitis was induced in ovariectomized (OVX) rats using a silk ligature. We then injected 5 μg of hIL-10 plasmid with 5 μl of liposomes or 5 μg of vector plasmid with 5 μl of liposomes into the palatal side of the gingival mucosa of the upper left second molar once every two days. The rats were killed 48 hours after the seventh injection. The body weight; bone mineral density of the whole body, pelvis and spine; resorption of the alveolar bone; and number of cytokine-positive cells were measured to determine the effects of hIL-10 on the periodontal tissue.
  
  
  RESULTS
  hIL-10 was expressed in periodontal tissues after local gene delivery. The expressed hIL-10 protein inhibited alveolar bone resorption and downregulated IL-1β, IL-6, TNF-α, RANKL and MMP-8 in the periodontal ligament in the root furcation region.
  
  
  CONCLUSIONS
  Local hIL-10 gene transfer suppressed alveolar bone resorption in OVX rats, and this effect was probably associated with the decline in the expression of pro-inflammatory cytokines in the periodontal tissues.
authors.affiliation: a School and Hospital of Stomatology , Fujian Medical University , Fuzhou , China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Li
authors.affiliation: a School and Hospital of Stomatology , Fujian Medical University , Fuzhou , China.
authors.forename: Souzhi
authors.initials: S
authors.lastname: Ma
authors.affiliation: a School and Hospital of Stomatology , Fujian Medical University , Fuzhou , China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Guo
authors.affiliation: a School and Hospital of Stomatology , Fujian Medical University , Fuzhou , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: a School and Hospital of Stomatology , Fujian Medical University , Fuzhou , China.
authors.forename: Kai
authors.initials: K
authors.lastname: Luo
authors.affiliation: b Nanjing Stomatological Hospital , Medical School of Nanjing University , Nanjing , China.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Yan
authors.affiliation: c Institute of Health and Biomedical Innovation, Queensland University of Technology , Brisbane , Australia.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D016301:Alveolar Bone Loss; D000818:Animals; D016207:Cytokines; D004195:Disease Models, Animal; D015316:Genetic Therapy; D005881:Gingiva / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics*; D010052:Ovariectomy; D010513:Periodontal Ligament / Q000378:metabolism; D010518:Periodontitis / Q000150:complications / Q000188:drug therapy* / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D016753:Interleukin-10
keywords: Cytokine; IL-10; gene transfer; osteoporosis; periodontal tissues