title: A classification system for zebrafish adipose tissues.
abstract: The zebrafish model system offers significant utility forin vivoimaging of adipose tissue (AT) dynamics and for screening to identify chemical and genetic modifiers of adiposity. In particular, AT can be quantified accurately in live zebrafish using fluorescent lipophilic dyes. Although this methodology offers considerable promise, the comprehensive identification and classification of zebrafish ATs has not been performed. Here, we use fluorescent lipophilic dyes andin vivoimaging systematically to identify, classify and quantify the zebrafish AT pool. We identify 34 regionally distinct zebrafish ATs, including five visceral ATs and 22 subcutaneous ATs. For each of these ATs, we describe detailed morphological characteristics to aid their identification in future studies. Furthermore, we quantify the areas for each AT and construct regression models to allow prediction of expected AT size and variation across a range of developmental stages. Finally, we demonstrate the utility of this resource for identifying effects of strain variation and high-fat diet on AT growth. Altogether, this resource provides foundational information on the identity, dynamics and expected quantities of zebrafish ATs for use as a reference for future studies.
authors.affiliation: Department of Molecular Genetics & Microbiology, Duke University, Durham, NC 27710, USA james.minchin@ed.ac.uk.|Department of Cell Biology & Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.|British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, Edinburgh EH16 4TJ, UK.
authors.forename: James E N
authors.identifier: 0000-0003-2316-1010
authors.initials: JEN
authors.lastname: Minchin
authors.affiliation: Department of Molecular Genetics & Microbiology, Duke University, Durham, NC 27710, USA.|Department of Cell Biology & Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: John F
authors.identifier: 0000-0002-5976-5206
authors.initials: JF
authors.lastname: Rawls
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology* / Q000378:metabolism*; D050154:Adiposity; D058500:Animal Fins / Q000502:physiology; D000818:Animals; D059305:Diet, High-Fat; D004625:Embryo, Nonmammalian / Q000378:metabolism; D009929:Organ Size; D012044:Regression Analysis; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*
keywords: Adipose tissue; Classification system; In vivo imaging; Zebrafish