title: Aptamer entrapment in microfluidic channel using one-step sol-gel process, in view of the integration of a new selective extraction phase for lab-on-a-chip.
abstract: There is a great demand for integrating sample treatment into μTASs. In this context, we developed a new sol-gel phase for extraction of trace compounds in complex matrices. For this purpose, the incorporation of aptamers in silica-based gel within PDMS/glass microfluidic channels was performed for the first time by a one-step sol-gel process. The effective gel attachment onto microchannel walls and aptamer incorporation in the polymerized gel were evaluated using fluorescence microscopy. A good gel stability and aptamer incorporation inside the microchannel was demonstrated upon rinsing and over storage time. The ability of gel-encapsulated aptamers to interact with its specific target (either sulforhodamine B as model fluorescent target, or diclofenac, a pain killer drug) was assessed too. The binding capacity of entrapped aptamers was quantified (in the micromolar range) and the selectivity of the interaction was evidenced. Preservation of aptamers binding affinity to target molecules was therefore demonstrated. Dissociation constant of the aptamer-target complex and interaction selectivity were evaluated similar to those in bulk solution. This opens the way to new selective on-chip SPE techniques for sample pretreatment.
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.|INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (U 1022), Paris, France.|CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Perréard
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.|INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (U 1022), Paris, France.|CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: d'Orlyé
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.|INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (U 1022), Paris, France.|CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Griveau
authors.affiliation: Department of Chemistry, Fudan University, Shanghai, P. R. China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.|INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (U 1022), Paris, France.|CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.
authors.forename: Fethi
authors.initials: F
authors.lastname: Bedioui
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.|INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (U 1022), Paris, France.|CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France.
authors.forename: Anne
authors.identifier: 0000-0001-9711-1919
authors.initials: A
authors.lastname: Varenne
mesh_terms: D000700:Analgesics / Q000737:chemistry; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods; D004008:Diclofenac / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D044367:Phase Transition; D012235:Rhodamines / Q000737:chemistry; D012680:Sensitivity and Specificity; D058428:Silica Gel / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012235:Rhodamines; D014874:Water Pollutants, Chemical; D004008:Diclofenac; C022027:lissamine rhodamine B; D058428:Silica Gel
keywords: Affinity; Aptamer; Electrophoretic microchannel; Fluorescence microscopy; Retention; Sol-gel