title: Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm.
abstract: Oil palm is the most productive oil crop in the world and composes 36% of the world production. However, the molecular mechanisms of hybrids vigor (or heterosis) between Dura, Pisifera and their hybrid progeny Tenera has not yet been well understood. Here we compared the temporal and spatial compositions of lipids and transcriptomes for two oil yielding organs mesocarp and endosperm from Dura, Pisifera and Tenera. Multiple lipid biosynthesis pathways are highly enriched in all non-additive expression pattern in endosperm, while cytokinine biosynthesis and cell cycle pathways are highly enriched both in endosperm and mesocarp. Compared with parental palms, the high oil content in Tenera was associated with much higher transcript levels of EgWRI1, homolog of Arabidopsis thaliana WRINKLED1. Among 338 identified genes in lipid synthesis, 207 (61%) has been identified to contain the WRI1 specific binding AW motif. We further functionally identified EgWRI1-1, one of three EgWRI1 orthologs, by genetic complementation of the Arabidopsis wri1 mutant. Ectopic expression of EgWRI1-1 in plant produced dramatically increased seed mass and oil content, with oil profile changed. Our findings provide an explanation for EgWRI1 as an important gene contributing hybrid vigor in lipid biosynthesis in oil palm.
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.|Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY, 10021, USA.|School of Computing, National University of Singapore, 117417, NUS, Singapore.|China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, 450001, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Jin
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.|State Key laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.affiliation: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
authors.forename: Rahmad
authors.initials: R
authors.lastname: Syah
authors.affiliation: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
authors.forename: Chin-Huat
authors.initials: CH
authors.lastname: Lim
authors.affiliation: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
authors.forename: Yuzer
authors.initials: Y
authors.lastname: Alfiko
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
authors.forename: Nur Estya Bte
authors.initials: NEB
authors.lastname: Rahman
authors.affiliation: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
authors.forename: Antonius
authors.initials: A
authors.lastname: Suwanto
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
authors.forename: Genhua
authors.initials: G
authors.lastname: Yue
authors.affiliation: School of Computing, National University of Singapore, 117417, NUS, Singapore.
authors.forename: Limsoon
authors.identifier: http://orcid.org/0000-0003-1241-5441
authors.initials: L
authors.lastname: Wong
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY, 10021, USA.
authors.forename: Nam-Hai
authors.initials: NH
authors.lastname: Chua
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore. jianye@im.ac.cn.|State Key laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. jianye@im.ac.cn.
authors.forename: Jian
authors.identifier: http://orcid.org/0000-0002-7741-4354
authors.initials: J
authors.lastname: Ye
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins; D028023:Arecaceae / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D002678:Chimera; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D006823:Hybrid Vigor; D008055:Lipids / Q000032:analysis; D000073878:Palm Oil / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency
chemical_list: D029681:Arabidopsis Proteins; D008055:Lipids; D014157:Transcription Factors; C495665:WRINKLED1 protein, Arabidopsis; D000073878:Palm Oil