title: Gelatinases A and B and Antioxidant Enzyme Activity in the Early Phase of Acute Myocardial Infarction.
abstract: Oxidative stress plays important roles in the pathophysiology of acute myocardial infarction (AMI). The aim of this study was to investigate the correlation of the oxidative stress status and matrix metalloproteinase activity in AMI patients in comparison to controls. This study included 136 subjects: 68 patients with AMI (42 males/26 females; mean age 58.5 ± 10.5 years) and 68 controls (37 males/29 females; mean age 60.2 ± 12.4 years). Gelatinases A and B were assayed using gelatin zymography, enzyme activities were obtained spectrophotometrically. Gelatinase A and B activities were increased in the AMI patients' group compared to the control. Activities of serum superoxide dismutase (SOD) and xanthine oxidase (XO) were significantly higher in AMI patients (106.53 ± 23.45 U/l, P < 0.001 and 158.18 ± 29.59 U/l, P < 0.001) than in the control group (55.99 ± 10.79 U/l and 79.81 ± 7.93 U/l). The activity of catalase (CAT) in the sera of AMI patients was lower (271.31 ± 7.53 U/l, P < 0.005) than in the control group (305.94 ± 97.28 U/l). Plasma glutathione peroxidase (GPx) and glutathione reductase (GR) in AMI patients were significantly higher (582.47 ± 184.81 U/l, P < 0.001 and 59.64 ± 21.88 U/l, P < 0.001) than in the control group (275.32 ± 104.69 U/l and 47.71 ± 20.05 U/l). The present findings demonstrate activation of gelatinases A and B and oxidative stress markers in the early stage of AMI. Gelatinases, detected at high levels in AMI patients only, indicate their noticeable predisposition for becoming additional biomarkers of the early phase of AMI.
authors.affiliation: Institute for Chemistry in Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: K
authors.initials: K
authors.lastname: Gopcevic
authors.affiliation: Centre for Endocrine Surgery, Clinical Centre of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: B
authors.initials: B
authors.lastname: Rovcanin
authors.affiliation: Institute for Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Kekic
authors.affiliation: Clinic for Cardiology, Clinical Centre of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Milasinovic
authors.affiliation: Institute for Biochemistry, Faculty of Medicine, University of Nis, Nis, Serbia.
authors.forename: G
authors.initials: G
authors.lastname: Kocic
authors.affiliation: Institute for Biochemistry, Faculty of Medicine, University of Nis, Nis, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Stojanovic
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D018093:Gelatinases / Q000097:blood / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000201:enzymology*; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D020033:Protein Isoforms / Q000097:blood; D012015:Reference Standards; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase / Q000097:blood
chemical_list: D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D020033:Protein Isoforms; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D005980:Glutathione Reductase; D018093:Gelatinases