title: Effects of benzo[a]pyrene dietary intake to antioxidative enzymes of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from unpolluted and polluted forests.
abstract: Anthropogenic activity in industrial development has imposed great threats to the environment and wildlife in the form of persistent organic pollutants. Polycyclic aromatic hydrocarbons (PAH) tend to accumulate in vegetation foliage which is the main food source of polyphagous insect species Lymantria dispar L. Origin and multigenerational adaptation of L. dispar population to environmental challenges strongly condition the enzymes' sensitivity to pollutants. In this study, our aim was to investigate response of the superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) to the chronic dietary exposure of benzo[a]pyrene in the midgut tissues and hemolymph of two L. dispar populations originating from unpolluted and polluted forest habitat. Midgut tissue of the larvae from the polluted forest showed significant increase in SOD, CAT and GST activity, while in unpolluted forest's larvae SOD and CAT showed elevated activities in hemolymph. L. dispar populations adapted to different level of pollution in their environment and expressed distinct tissue-dependent antioxidative enzyme sensitivity to benzo[a]pyrene diet, implying high potential for further elucidation of these enzymes as molecular biomarkers.
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia. Electronic address: anja.gavrilovic@ibiss.bg.ac.rs.
authors.forename: Anja
authors.initials: A
authors.lastname: Gavrilović
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Larisa
authors.initials: L
authors.lastname: Ilijin
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Mrdaković
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Vlahović
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mrkonja
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Matić
authors.affiliation: Department of Insect Physiology and Biochemistry, University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Perić-Mataruga
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000494:pharmacology*; D004781:Environmental Exposure; D004787:Environmental Pollution / Q000009:adverse effects*; D065928:Forests; D006458:Hemolymph / Q000201:enzymology; D007814:Larva / Q000187:drug effects* / Q000201:enzymology; D009036:Moths / Q000187:drug effects / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis / Q000187:drug effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis
chemical_list: D000975:Antioxidants; D011084:Polycyclic Aromatic Hydrocarbons; D001564:Benzo(a)pyrene; D010088:Oxidoreductases
keywords: Antioxidative enzymes; Benzo[a]pyrene; Hemolymph; Lymantria dispar L.; Midgut; Unpolluted and polluted forest population