title: Biomimetic ELISA detection of malachite green based on molecularly imprinted polymer film.
abstract: A highly selective and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of malachite green (MG) using a molecularly imprinted polymer (MIP) film as bionic antibody. The MIP film, based on the self-polymerization of dopamine, was fabricated on the surfaces of a 96-well microplate. It showed specific recognition for MG in aqueous solution. A direct competitive ELISA method was established with the sensitivity reaching 10.31μgL-1and the detection limit being 0.3μgL-1. The cross-reactivity of two structural analogues to MG was less than 10%. The average recovery tested by MG standard spiking was 88.8% for bass and 90.4% for water, and the relative standard deviations were less than 3.6%. All the above results indicated that the developed method could be used to detect MG in fish and water samples rapidly, specifically and accurately.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Ai-Hong
authors.initials: AH
authors.lastname: Peng
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Zheng-Zhong
authors.initials: ZZ
authors.lastname: Lin
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zhong
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Chen
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361102, China. Electronic address: zhyhuang@jmu.edu.cn.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Huang
mesh_terms: D000818:Animals; D000906:Antibodies; D032701:Biomimetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005396:Fish Products; D005399:Fishes; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D011108:Polymers; D012394:Rosaniline Dyes; C005095:malachite green
keywords: Bionic antibody; ELISA; Fish; Malachite green; Molecularly imprinted polymer film; Water