title: Targeting of Apoptotic Cells Using Functionalized Fe₂O₃ Nanoparticles.
abstract: Fe₂O₃ nanoparticles (NPs) have been synthesized and functionalized with SiO₂ and -NH₂ group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to beca.80% for Fe₂O₃-SiO-NH₂ functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs)in vivoandin vitrohas been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan. mmekawy@zewailcity.edu.eg.|Center of Materials Science, Zewail City of Science and Technology, Sheikh Zayed District, 12588, 6th of October City, Giza, Egypt. mmekawy@zewailcity.edu.eg.
authors.forename: Moataz
authors.initials: M
authors.lastname: Mekawy
authors.affiliation: Department of Neurosurgery, Aomori Prefecture Central Hospital, 2 1 1 Higashitsukurimichi, Aomori 0308553, Japan. satsushi2002@yahoo.co.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Akita University, 1-1-1 Hondo, Akita 010-8543, Japan. hshim@nsg.med.akita-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: Department of Neurosurgery, Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan. tomi@nsg.med.tohoku.ac.jp.
authors.forename: Teiji
authors.initials: T
authors.lastname: Tominaga
keywords: apoptosis; brain diagnosis; fluorescently-labeled poly-caspase inhibitor (SR-FLIVO); functionalized nanoparticles (FNPs)