title: A furoviral replicase recruits host HSP70 to membranes for viral RNA replication.
abstract: Many host factors have been identified to be involved in viral infection. However, although furoviruses cause important diseases of cereals worldwide, no host factors have yet been identified that interact with furoviral genes or participate in the viral infection cycle. In this study, both TaHSP70 and NbHSP70 were up-regulated in Chinese wheat mosaic furovirus (CWMV)-infected plants. Their overexpression and inhibition were correlated with the accumulation of viral genomic RNAs, suggesting that the HSP70 genes could be necessary for CWMV infection. The subcellular distributions of TaHSP70 and NbHSP70 were significantly affected by CWMV infection or by infiltration of RNA1 alone. Further assays showed that the viral replicase encoded by CWMV RNA1 interacts with both TaHSP70 and NbHSP70 in vivo and vitro and that its region aa167-333 was responsible for the interaction. Subcellular assays showed that the viral replicase could recruit both TaHSP70 and NbHSP70 from the cytoplasm or nucleus to the granular aggregations or inclusion-like structures on the intracellular membrane system, suggesting that both HSP70s may be recruited into the viral replication complex (VRC) to promote furoviral replication. This is the first host factor identified to be involved in furoviral infection, which extends the list and functional scope of HSP70 chaperones.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.|College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.|College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Nian-Jun
authors.initials: NJ
authors.lastname: Cai
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.|Zhejiang Agriculture and Forest University, Linan 311300, China.
authors.forename: Ne
authors.initials: N
authors.lastname: Wu
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.|Zhejiang Agriculture and Forest University, Linan 311300, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Zhumadian Academy of Agriculture Sciences, Zhumadian 463000, China.
authors.forename: Xiang-Feng
authors.initials: XF
authors.lastname: Meng
authors.affiliation: Zhumadian Academy of Agriculture Sciences, Zhumadian 463000, China.
authors.forename: Tong-Quan
authors.initials: TQ
authors.lastname: Zhu
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control; MOA and Zhejiang Key Laboratory of Plant Protection and Biotechnology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.|College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
authors.forename: Heng-Mu
authors.initials: HM
authors.lastname: Zhang
mesh_terms: D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D014908:Triticum / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D014779:Virus Replication
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase