title: Biosynthetic pathways of aminoglycosides and their engineering.
abstract: Despite decades long clinical usage, aminoglycosides still remain a valuable pharmaceutical source for fighting Gram-negative bacterial pathogens, and their newly identified bioactivities are also renewing interest in this old class of antibiotics. As Nature's gift, some aminoglycosides possess natural defensive structural elements that can circumvent drug resistance mechanisms. Thus, a detailed understanding of aminoglycoside biosynthesis will enable us to apply Nature's biosynthetic strategy towards expanding structural diversity in order to produce novel and more robust aminoglycoside analogs. The engineered biosynthesis of novel aminoglycosides is required not only to develop effective therapeutics against the emerging 'superbugs' but also to reinvigorate antibiotic lead discovery in readiness for the emerging post-antibiotic era.
authors.affiliation: School of Biosystem and Biomedical Science, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Je Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Ban
authors.affiliation: Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.
authors.forename: Sang-Jip
authors.initials: SJ
authors.lastname: Nam
authors.affiliation: Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.
authors.forename: Sun-Shin
authors.initials: SS
authors.lastname: Cha
authors.affiliation: Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea. Electronic address: joonyoon@ewha.ac.kr.
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
mesh_terms: D000617:Aminoglycosides / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification; D053898:Biosynthetic Pathways / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents