title: Psoralen Inhibited Apoptosis of Osteoporotic Osteoblasts by Modulating IRE1-ASK1-JNK Pathway.
abstract: Osteoporosis is a common disease causing fracture in older populations. Abnormal apoptosis of osteoblasts contributes to the genesis of osteoporosis. Inhibiting apoptosis of osteoblasts provides a promising strategy to prevent osteoporosis. The proliferation of osteoblasts isolated from osteoporotic patients or healthy subjects was determined by MTT assay. Apoptosis was determined by Annexin V/PI assay. Protein expression was measured by western blot. The proliferation of osteoblasts isolated from osteoporotic patients was inhibited and the apoptosis level of these cells was higher than the osteoblasts from healthy subjects. Incubation with psoralen or estradiol significantly enhanced the proliferation and decreased the apoptosis level of osteoporotic osteoblasts. Western blot demonstrated that psoralen or estradiol treatment downregulated the expression of IRE1, p-ASK, p-JNK, and Bax. Meanwhile, expression of Bcl-2 was upregulated. Pretreatment by IRE1 agonist tunicamycin or JNK agonist anisomycin attenuated the effect of psoralen on osteoporotic osteoblasts. Psoralen inhibited apoptosis of osteoporotic osteoblasts by regulating IRE1-ASK1-JNK pathway.
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Houming
authors.identifier: 0000-0003-2851-0187
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Xinlin
authors.identifier: 0000-0003-0931-392X
authors.initials: X
authors.lastname: Wu
mesh_terms: D000841:Anisomycin / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D004722:Endoribonucleases / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005363:Ficusin / Q000008:administration & dosage*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000096:biosynthesis / Q000235:genetics*; D048848:MAP Kinase Kinase Kinase 5 / Q000096:biosynthesis / Q000235:genetics*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008875:Middle Aged; D010006:Osteoblasts / Q000187:drug effects; D010024:Osteoporosis / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D061251:Primary Cell Culture; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D014415:Tunicamycin / Q000008:administration & dosage; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D014415:Tunicamycin; D000841:Anisomycin; C528229:ERN1 protein, human; D017346:Protein Serine-Threonine Kinases; D048848:MAP Kinase Kinase Kinase 5; C482858:MAP3K5 protein, human; D048670:MAP Kinase Kinase 4; D004722:Endoribonucleases; D005363:Ficusin