title: Genetic diversity and relatedness between Canindé and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers.
abstract: The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers resulted in a total number of 145 alleles, with an average of 8.5 alleles per locus. The observed and expected heterozygosities were ≥0.687 and ≥0.627, respectively, for all loci. The polymorphic information content showed that all loci were highly informative with an overall mean of 0.757. Overall FSTacross all populations and loci was 18%, which was consistent with the coefficient of gene differentiation (GST= 0.104). AMOVA revealed that 12.8% of the variation was captured between breeds. The Bayesian STRUCTURE clustering detected the maximum likelihood for a model of two genetically distinct groups, in agreement with the number of predefined studied breeds and the two-dimensional plot from the PCoA analysis. The exotic British Alpine breed and the naturalized Brazilian Canindé breed were clearly differentiated by the microsatellite markers, indicating that these two breeds have distant genetic identities, despite the phenotypic similarity.
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, CE, Brasil talitavet2003@gmail.com.|Laboratório de Tecnologia do Sêmen Caprino e Ovino, Universidade Estadual do Ceará, Fortaleza, CE, Brasil talitavet2003@gmail.com.
authors.forename: T S
authors.initials: TS
authors.lastname: Câmara
authors.affiliation: Laboratório de Tecnologia do Sêmen Caprino e Ovino, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
authors.forename: J F
authors.initials: JF
authors.lastname: Nunes
authors.affiliation: Embrapa Caprinos e Ovinos, Sobral, CE, Brasil.
authors.forename: F M
authors.initials: FM
authors.lastname: Diniz
authors.affiliation: Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portella, Teresina, PI, Brasil.
authors.forename: G R
authors.initials: GR
authors.lastname: Silva
authors.affiliation: Embrapa Meio-Norte, Teresina, PI, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: Araújo
mesh_terms: D000483:Alleles; D000818:Animals; D001938:Brazil; D001947:Breeding; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D040961:Genetic Drift; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D006041:Goats / Q000235:genetics*; D006579:Heterozygote; D018895:Microsatellite Repeats
chemical_list: D004247:DNA