title: Proteomic Biomarker Identification in Cerebrospinal Fluid for Leptomeningeal Metastases with Neurological Complications.
abstract: Leptomeningeal metastases (LM) from solid tumours, lymphoma and leukaemia are characterized by multifocal neurological deficits with a high mortality rate. Early diagnosis and initiation of treatment are essential to kerb neurological deterioration. However, this is not always possible as 25% of cerebrospinal fluid samples produce false-negative results at first cytological examination. The identification of biomarkers that allow stratification of individuals according to risk for developing LM would be a major benefit. Proteomic-based approaches are now in increasing use for this purpose, and these are reviewed in this chapter with a focus on cerebrospinal fluid (CSF) analyses. The construction of a CSF proteome disease database would also facilitate analysis of other neurological disorders.
authors.affiliation: Proteomics Unit, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain.
authors.forename: Norma
authors.initials: N
authors.lastname: Galicia
authors.affiliation: Proteomics Unit, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain.|Department of Medicine and General Service of Cytometry, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Proteomics Unit, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Dégano
authors.affiliation: Laboratory of Neuroproteomics, Institute of Biology, University of Campinas, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Proteomics Unit, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain.
authors.forename: Nieves
authors.initials: N
authors.lastname: Ibarrola
authors.affiliation: Proteomics Unit, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain. mfuentes@usal.es.|Department of Medicine and General Service of Cytometry, Cancer Research Centre, IBSAL, University of Salamanca-CSIC, Campus Miguel de Unamuno s/n, 37007, Salamanca, Spain. mfuentes@usal.es.
authors.forename: Manuel
authors.initials: M
authors.lastname: Fuentes
mesh_terms: D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D002277:Carcinoma / Q000134:cerebrospinal fluid / Q000150:complications / Q000556:secondary*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D005544:Forecasting; D006801:Humans; D008223:Lymphoma / Q000134:cerebrospinal fluid; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid / Q000150:complications / Q000556:secondary*; D001523:Mental Disorders / Q000134:cerebrospinal fluid / Q000209:etiology; D009363:Neoplasm Proteins / Q000134:cerebrospinal fluid*; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D002556:Cerebrospinal Fluid Proteins; D009363:Neoplasm Proteins
keywords: Biomarkers; Cerebrospinal fluid; Leptomeningeal metastases; Mass spectrometry; Proteomics