title: Species-specific detection of processed animal proteins in feed by Raman spectroscopy.
abstract: The existing European Regulation (EC n° 51/2013) prohibits the use of animals meals in feedstuffs in order to prevent Bovine Spongiform Encephalopathy infection and diffusion, however the legislation is rapidly moving towards a partial lifting of the "feed ban" and the competent control organisms are urged to develop suitable analytical methods able to avoid food safety incidents related to animal origin products. The limitations of the official methods (i.e. light microscopy and Polymerase Chain Reaction) suggest exploring new analytic ways to get reliable results in a short time. The combination of spectroscopic techniques with optical microscopy allows the development of an individual particle method able to meet both selectivity and sensitivity requirements (0.1%w/w). A spectroscopic method based on Fourier Transform micro-Raman spectroscopy coupled with Discriminant Analysis is here presented. This approach could be very useful for in-situ applications, such as customs inspections, since it drastically reduces time and costs of analysis.
authors.affiliation: Department of Drug Science and Technology, Università degli Studi di Torino, Via Giuria 9, 10125 Torino, Italy. Electronic address: l.mandrile@inrim.it.
authors.forename: Luisa
authors.initials: L
authors.lastname: Mandrile
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Torino, Italy. Electronic address: Giuseppina.Amato@izsto.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Amato
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Torino, Italy. Electronic address: Daniela.Marchis@izsto.it.
authors.forename: Daniela
authors.initials: D
authors.lastname: Marchis
authors.affiliation: Department of Drug Science and Technology, Università degli Studi di Torino, Via Giuria 9, 10125 Torino, Italy. Electronic address: gianmario.martra@unito.it.
authors.forename: Gianmario
authors.initials: G
authors.lastname: Martra
authors.affiliation: Thermodynamic Division, Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce, 91, 10135 Torino, Italy. Electronic address: a.rossi@inrim.it.
authors.forename: Andrea Mario
authors.initials: AM
authors.lastname: Rossi
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D013045:Species Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*
keywords: Chemometrics; PAPs; Raman spectroscopy; Species identification