title: Genetic Variants of Cytochrome b-245, Alpha Polypeptide Gene and Premature Acute Myocardial Infarction Risk in an Iranian Population.
abstract: BACKGROUND
  Oxidative stress induced by superoxide anion plays critical roles in the pathogenesis of coronary artery disease (CAD) and hence acute myocardial infarction (AMI). The major source of superoxide production in vascular smooth muscle and endothelial cells is the NADPH oxidase complex. An essential component of this complex is p22phox, that is encoded by the cytochrome b-245, alpha polypeptide (CYBA) gene. The aim of this study was to investigate the association of CYBA variants (rs1049255 and rs4673) and premature acute myocardial infarction risk in an Iranian population.
  
  
  METHODS
  The study population consisted of 158 patients under the age of 50 years, with a diagnosis of premature AMI, and 168 age-matched controls with normal coronary angiograms. Genotyping of the polymorphisms was performed by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  There was no association between the genotypes and allele frequencies of rs4673 polymorphism and premature acute myocardial infarction (P>0.05). A significant statistical association was observed between the genotypes distribution of rs1049255 polymorphism and AMI risk (P=0.037). Furthermore, the distribution of AA+AG/GG genotypes was found to be statistically significant between the two groups (P=0.011).
  
  
  CONCLUSIONS
  Our findings indicated that rs1049255 but not rs4673 polymorphism is associated with premature AMI.
authors.affiliation: Department of Physiology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Amin
authors.affiliation: Faculty of Veterinary Science, Shahrekord Islamic Azad University, Shahrekord, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Jahani
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Ghaedi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Alipoor
authors.affiliation: Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Fatemi
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Michael
authors.initials: M
authors.lastname: Tajik
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Sharifi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Golmohammadi
authors.affiliation: Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Askari
authors.affiliation: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Asaad
authors.initials: A
authors.lastname: Azarnejad
authors.affiliation: Department of Nutrition, School of Health, Yasouj University of Medical Sciences, Yasouj, Iran.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Alipoor
authors.affiliation: Health Center Baghmalek, School of Health, Ahvaz University of Medical Sciences, Ahvaz, Iran.
authors.forename: Aliasghar
authors.initials: A
authors.lastname: Valipour
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Mousavizadeh
keywords: acute myocardial infarction; p22phox; polymorphism; rs1049255; rs4673