title: New tools for non-invasive exploration of collagen network in cartilaginous tissue-engineered substitute.
abstract: In tissue engineering approaches, the quality of substitutes is a key element to determine its ability to treat cartilage defects. However, in clinical practice, the evaluation of tissue-engineered cartilage substitute quality is not possible due to the invasiveness of the standard procedure, which is to date histology. The aim of this work was to validate a new innovative system performed from two-photon excitation laser adapted to an optical macroscope to evaluate at macroscopic scale the collagen network in cartilage tissue-engineered substitutes in confrontation with gold standard histologic techniques or immunohistochemistry to visualize type II collagen. This system permitted to differentiate the quality of collagen network between ITS and TGF-β1 treatments. Multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) at macroscopical scale represent an innovative and non-invasive technique to monitor the quality of collagen network in cartilage tissue-engineered substitutes before in vivo implantation.
authors.affiliation: Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Henrionnet
authors.affiliation: Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.|Plateforme d'Imagerie Cellulaire PTIBC-IBISA, FR3209 CNRS, Faculté de Médecine, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Dumas
authors.affiliation: Plateforme d'Imagerie Cellulaire PTIBC-IBISA, FR3209 CNRS, Faculté de Médecine, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Hupont
authors.affiliation: Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Jean François
authors.initials: JF
authors.lastname: Stoltz
authors.affiliation: Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.|Plateforme d'Imagerie Cellulaire PTIBC-IBISA, FR3209 CNRS, Faculté de Médecine, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.|Service de Chirurgie Orthopédique et Traumatologique, CHRU Nancy, 29 avenue du Maréchal de Lattre de Tassigny, CO60034, F54035 Nancy, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Mainard
authors.affiliation: Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Gillet
authors.affiliation: Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
authors.forename: Astrid
authors.initials: A
authors.lastname: Pinzano
mesh_terms: D002356:Cartilage / Q000033:anatomy & histology* / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis; D024043:Collagen Type II / Q000032:analysis*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D024043:Collagen Type II; D053773:Transforming Growth Factor beta1
keywords: Cartilage; mesenchymal stem cells; second harmonic generation; tissue-engineered substitute