title: Assessing the potential of angiotensin II type 1 receptor and donor specific anti-endothelial cell antibodies to predict long-term kidney graft outcome.
abstract: Endothelial cell antigens have been reported as potential targets for antibodies in the context of organ transplantation, leading to increased risk for graft failure. Serum samples from 142 consecutive living donor kidney recipients were tested for the presence of antibodies to angiotensin II - type 1 receptor (AT1R), donor endothelial cells, and donor HLA. Graft survival was monitored for five years post-transplant, and secondary outcomes, including biopsy-proven rejection, proteinuria, biopsy-proven vasculopathy, and renal function based on serum creatinine were also assessed for the first two to three years. AT1R antibody levels were positive (>17U/mL) in 11.3%, 18.8% and 8.1% of patients pre-transplant, post-transplant and at time of indication biopsy, respectively. XM-ONE assay was positive in 17.6% of patients pre-transplant (7 IgG+; 15 IgM+; 3 IgG+/IgM+). Overall, 4 patients experienced antibody-mediated rejection (AMR), 31 borderline cellular rejection (BCR), 19 cellular rejection (CR) and 3 mixed AMR and CR within the first 24months. While pre-existing and de novo donor-specific HLA antibodies were associated with graft failure and many secondary outcomes, no statistical association was found for either anti-endothelial or anti-AT1R antibodies, indicating that these tests may not be the best predictors of graft outcome in living donor renal transplantation.
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA. Electronic address: david.pinelli@northwestern.edu.
authors.forename: David F
authors.initials: DF
authors.lastname: Pinelli
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA; Northwestern University, Division of Nephrology and Hypertension, Department of Medicine, 251 East Huron Street, Galter Suite 3-150, Chicago, IL 60611, USA. Electronic address: John.Friedewald@nm.org.
authors.forename: John J
authors.initials: JJ
authors.lastname: Friedewald
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA. Electronic address: kelley.haarberg@uhs-sa.com.
authors.forename: Kelley M K
authors.initials: KMK
authors.lastname: Haarberg
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA. Electronic address: shree.radhakrishnan@northwestern.edu.
authors.forename: Shree L
authors.initials: SL
authors.lastname: Radhakrishnan
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA. Electronic address: jenniferzitzner@gmail.com.
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Zitzner
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA. Electronic address: wendy.wegner@duke.edu.
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Hanshew
authors.affiliation: Northwestern University Feinberg School of Medicine, Comprehensive Transplant Center, Division of Transplant Surgery, 303 E Chicago Ave, Tarry Building Suite 11-763, Chicago, IL 60611, USA. Electronic address: a-tambur@northwestern.edu.
authors.forename: Anat R
authors.initials: AR
authors.lastname: Tambur
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood*; D042783:Endothelial Cells / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis*; D006801:Humans; D007111:Immunity, Cellular; D016030:Kidney Transplantation; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D009928:Organ Specificity; D011237:Predictive Value of Tests; D011379:Prognosis; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000906:Antibodies; D044140:Receptor, Angiotensin, Type 1
keywords: Antibodies; Endothelial cells; HLA; Transplant