title: Suppression of p53-inducible gene 3 is significant for glioblastoma progression and predicts poor patient prognosis.
abstract: Glioblastoma is the most malignant and invasive brain tumor with extremely poor prognosis. p53-inducible gene 3, a downstream molecule of the tumor suppressor p53, has been found involved in apoptosis and oxidative stress response. However, the functions of p53-inducible gene 3(PIG3) in cancer are far from clear including glioblastoma. In this study, we found that p53-inducible gene 3 expression was suppressed in glioblastoma tissues compared with normal tissues. And the expression of p53-inducible gene 3 was significantly associated with the World Health Organization grade. Patients with high p53-inducible gene 3 expression have a significantly longer median survival time (15 months) than those with low p53-inducible gene 3 expression (8 months). According to Cox regression analysis, p53-inducible gene 3 was an independent prognostic factor with multivariate hazard ratio of 0.578 (95% confidence interval, 0.352-0.947; p = 0.030) for overall survival. Additionally, gain and loss of function experiments showed that knockdown of p53-inducible gene 3 significantly increased the proliferation and invasion ability of glioblastoma cells while overexpression of p53-inducible gene 3 inhibited the proliferation and invasion ability. The results of in vivo glioblastoma models further confirmed that p53-inducible gene 3 suppression promoted glioblastoma progression. Altogether, our data suggest that high expression of p53-inducible gene 3 is significant for glioblastoma inhibition and p53-inducible gene 3 independently indicates good prognosis in patients, which might be a novel prognostic biomarker or potential therapeutic target in glioblastoma.
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, Yanbian University Medical College, Yanji, China.
authors.forename: Jishu
authors.initials: J
authors.lastname: Quan
authors.affiliation: 2 Department of Pediatrics, Yanbian Maternity and Child Care Center, Yanji, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, Yanbian University Medical College, Yanji, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Jin
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, Yanbian University Medical College, Yanji, China.
authors.forename: Dunfu
authors.initials: D
authors.lastname: Chen
authors.affiliation: 3 Yanbian University Hospital, Yanji, China.
authors.forename: Xuezhe
authors.initials: X
authors.lastname: Yin
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, Yanbian University Medical College, Yanji, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002648:Child; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D008875:Middle Aged; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D011518:Proto-Oncogene Proteins; C495901:TP53 protein, human; C411296:TP53I3 protein, human; D016159:Tumor Suppressor Protein p53
keywords: glioblastoma; invasion; p53-inducible gene 3; prognosis