title: Role of microRNA-130a in myocardial hypoxia/reoxygenation injury.
abstract: The aim of this study was to investigate the role of microRNA (miR)-130a in the pathogenesis of myocardial hypoxia/reoxygenation (H/R) injury. Primary rat cardiomyocytes were cultured and subjected to H/R treatment. Reverse transcription-quantitative polymerase chain reaction was performed to detect the levels of miR-130a, western blot analysis was used to determine the expression of various proteins, and CCK-8 assay was performed to determine cell viability. In addition, flow cytometry was used to assess apoptosis. The cell viability was significantly decreased and the apoptosis rate was significantly increased in H/R-treated primary cardiomyocytes, and the expression level of miR-130a was also elevated in these model cells. Transfection with miR-130a inhibitor significantly elevated the cell viability and reduced the apoptosis rate in H/R-treated cardiomyocytes. Bioinformatics analysis indicated that autophagy-related gene 14 (ATG14) is the target for miR-130a, which was confirmed by dual-luciferase reporter assay and western blot analysis. When the H/R model cells were co-transfected with miR-130a inhibitor and small interfering RNA against ATG14, the cell viability was significantly reduced and the apoptosis rate was significantly elevated, compared with that of cells transfected with miR-130a inhibitor alone. miR-130a inhibitor transfection significantly elevated the levels of ATG14 and phosphorylated (p-)Beclin 1, increased the LC3II/LC3I ratio, and decreased the expression levels of P62 and cleaved caspase-3, while the co-transfection of miR-130a inhibitor and siR-ATG14 attenuated these effects in H/R-induced primary cardiomyocytes. These results indicate that miR-130a is involved in H/R-induced injuries in primary cardiomyocytes, and that the inhibition of miR-130a increases the levels of ATG14 and p-Beclin 1, thereby increasing autophagy and inhibiting apoptosis in these cells.
authors.affiliation: Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huan
authors.affiliation: Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Endocrinology, Traditional Chinese Medical Hospital of Laiwu, Laiwu, Shandong 271100, P.R. China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Qin
authors.affiliation: Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Zengtang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Cardiac Function Laboratory, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Junye
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Sino-German Laboratory, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100037, P.R. China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
keywords: apoptosis; autophagy; autophagy-related gene 14; hypoxia/reoxygenation; miR–130a; microRNA