title: Enrichment of apoplastic fluid with therapeutic recombinant protein for efficient biofarming.
abstract: OBJECTIVE
  For efficient biofarming we attempted to enrich plant interstitial fluid (IF)/apoplastic fluid with targeted recombinant therapeutic protein. We employed a synthetic human Glucocerebrosidase (GCB), a model biopharmaceutical protein gene in this study.
  
  
  RESULTS
  Twenty one Nicotiana varieties, species and hybrids were initially screened for individual IF recovery and based on the findings, we selected Nicotiana tabacum NN (S-9-6), Nicotiana tabacum nn (S-9-7) and Nicotiana benthamiana (S-6-6) as model plants for raising transgenic expressing GCB via Agrobacterium mediated transformation under the control of M24 promoter; GCB specific activity in each transgenic lines were analyzed and we observed higher concentration of recombinant GCB in IF of these transgenic lines (S-9-6, S-9-7, and S-6-6) in comparison to their concentration in crude leaf extracts.
  
  
  CONCLUSIONS
  Recovery of valuable therapeutics in plant IF as shown in the present study holds great promise for promoting plant based biofarming. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:726-736, 2017.
authors.affiliation: Dept. of Molecular Plant Virology and Plant Genetic Engineering, KTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546-0236.
authors.forename: Aparajita
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Dept. of Molecular Plant Virology and Plant Genetic Engineering, KTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546-0236.
authors.forename: Narayan C
authors.initials: NC
authors.lastname: Das
authors.affiliation: Dept. of Molecular Plant Virology and Plant Genetic Engineering, KTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546-0236.
authors.forename: Sumita
authors.initials: S
authors.lastname: Raha
authors.affiliation: Dept. of Molecular Plant Virology and Plant Genetic Engineering, KTRDC, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546-0236.
authors.forename: Indu B
authors.initials: IB
authors.lastname: Maiti
authors.affiliation: Dept. of Gene Function and Regulation, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.|Dept. of Biotechnology, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Shrestha
authors.affiliation: Dept. of Gene Function and Regulation, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.|Dept. of Biotechnology, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.
authors.forename: Ahamed
authors.initials: A
authors.lastname: Khan
authors.affiliation: Dept. of Gene Function and Regulation, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.|Dept. of Biotechnology, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.
authors.forename: Sefali
authors.initials: S
authors.lastname: Acharya
authors.affiliation: Dept. of Gene Function and Regulation, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.|Dept. of Biotechnology, Institute of Life Sciences, Government of India, Chandrasekharpur, Bhubaneswar, Odisha, India.
authors.forename: Nrisingha
authors.initials: N
authors.lastname: Dey
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D005962:Glucosylceramidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010936:Plant Extracts / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011994:Recombinant Proteins; D005962:Glucosylceramidase
keywords: GCB; Gaucher's disease; PMP; biofarming; interstitial fluid