title: Biosensing platform for the detection of uric acid based on graphene quantum dots and G-quadruplex/hemin DNAzyme.
abstract: In this paper, a label-free biosensing platform for fluorescence detection of uric acid was designed on the peroxidase-mimicking activities of G-quadruplex/hemin DNAzyme and the introduction of caffeic acid. Uric acid could be decomposed by uricase and then produced hydrogen peroxide and allantoin. We thus successfully achieved the indirect detection of uric acid by monitoring the concentration of hydrogen peroxide. The G-quadruplex/hemin DNAzyme could act as peroxidase and decompose the hydrogen peroxide into hydroxyl radicals at room temperature. Due to the strong oxidizing of hydroxyl radicals, caffeic acid was converted to corresponding quinone, thus leading to fluorescence quenching of GQDs. Under the optimized experimental conditions, the quenched fluorescence intensity was linearly relative to the concentration of uric acid, ranging from 2 μM to 300 μM with a detection limit of 500 nM. The applicability of proposed method was further proved with satisfactory results in human serum and urine samples.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Cai
authors.affiliation: Nursing Department, Children's Hospital of Changchun City, Changchun, 130051, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xia
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D015374:Biosensing Techniques; D021881:DNA, Catalytic; D054856:G-Quadruplexes; D006108:Graphite; D006427:Hemin; D006801:Humans; D006861:Hydrogen Peroxide; D045663:Quantum Dots; D014527:Uric Acid / Q000097:blood* / Q000652:urine*
chemical_list: D021881:DNA, Catalytic; D014527:Uric Acid; D006427:Hemin; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Caffeic acid; Fluorescence detection; G-quadruplex/hemin DNAzyme; Graphene quantum dots; Uric acid