title: Larvicidal activity ofOttonia anisummetabolites againstAedes aegypti: A potential natural alternative source for mosquito vector control in Brazil.
abstract: OBJECTIVE
  Aedes aegypti mosquito is the principal vector of the viruses responsible for urban yellow fever, dengue, dengue haemorrhagic fever, as well as Zika and chikungunya in Brazil. The present study was aimed to investigate the insecticidal potential of the extract and fractions of Ottonia anisum, along with special metabolites isolated from it, as natural alternatives against larvae (L3) of Ae. aegypti, vector of potentially deadly tropical infections in Brazil.
  
  
  METHODS
  The plant species O. anisum was collected in March 2015, at Xerιm area, in Rio de Janeiro City, Brazil. Crude extracts and the isolated pure compounds were screened for toxicity against Ae. aegypti larvae (L3). Bioassays were performed on 20 larvae (L3) of Ae. aegypti in triplicate. The samples were dissolved in a mixture of acetone and DMSO at final concentrations of 1-200 μg/ml. The toxicity of the solutions was evaluated towards the growth and development of Ae. aegypti larvae till emergence of adults.
  
  
  RESULTS
  The crude hexane extract showed 100% larval mortality 24 h after treatment at a concentration of 200 μg/ml. The bioassays using 1-butyl-3,4-methylenedioxybenzene revealed 100% mortality among L3 larvae, 24 h afterthe treatment at a concentration of 30 μg/ml, the LC recorded was 1.6 μg/ml. At concentration of 10 μg/ml, the L3 larval mortality recorded was 92%.
  
  
  CONCLUSIONS
  The metabolite 1-butyl-3,4-methylenedioxybenzene showed potent toxicity against Ae. aegypti larvae. This arylbutanoid agent could be used as a natural alternative adjuvant pesticide, in new compositions that would be environmentally safer.
authors.affiliation: Walter Mors Institute for Research on Natural Products (IPPN), Health Sciences Center, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: André M
authors.initials: AM
authors.lastname: Marques
authors.affiliation: Walter Mors Institute for Research on Natural Products (IPPN), Health Sciences Center, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Leosvaldo S
authors.initials: LS
authors.lastname: Velozo
authors.affiliation: Vector Insect Laboratory, Universidade Severino Sombra, Av. Expedicionário Oswaldo de Almeida Ramos, Rio de Janeiro, Brazil.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Carvalho
authors.affiliation: Vector Insect Laboratory, Universidade Severino Sombra, Av. Expedicionário Oswaldo de Almeida Ramos, Rio de Janeiro; Interdisciplinary Laboratory for Entomological Surveillance of Diptera and Hemiptera, Brazil.
authors.forename: Michele T
authors.initials: MT
authors.lastname: Serdeiro
authors.affiliation: Sentinel Operational Center for Vector Mosquitoes; Hematozoa Transmission Mosquitoes Laboratory (LATHEMA), Oswaldo Cruz Institute, Fiocruz, Brazil.
authors.forename: Nildimar A
authors.initials: NA
authors.lastname: Honório
authors.affiliation: Walter Mors Institute for Research on Natural Products (IPPN), Health Sciences Center, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Maria Auxiliadora C
authors.initials: MA
authors.lastname: Kaplan
authors.affiliation: Vector Insect Laboratory, Universidade Severino Sombra, Av. Expedicionário Oswaldo de Almeida Ramos, Rio de Janeiro; Professional Master's Course on Environmental Sciences and Professional Master's Course on Health Sciences, Universidade Severino Sombra, Av. Expedicionário Oswaldo de Almeida Ramos, Vassouras; Laboratório de Entomologia Médica e Forense, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Marise
authors.initials: M
authors.lastname: Maleck
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001681:Biological Assay; D001938:Brazil; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D000072138:Mosquito Vectors; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D007306:Insecticides; D064209:Phytochemicals; D010936:Plant Extracts