title: Bone Marrow-Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules.
abstract: OBJECTIVE
  We investigated the regenerative capacity of intravenous administration of bone marrow-derived mononuclear cells (BMMCs) in a rat model of bilateral renal ischemia/reperfusion (IR) injury and the involvement of inflammatory anti-inflammatory and other biological markers in this process.
  
  
  METHODS
  Rats were subjected to 1h bilateral renal pedicle clamping. BMMCs were injected i.v 1h after reperfusion and tracked by 99mTc and GFP+ BMMCs. Twenty-four hours after reperfusion, renal function and histological changes were evaluated. The mRNA (real time PCR) and protein (ELISA and immuno-staining) expression of biological markers were analyzed.
  
  
  RESULTS
  Renal function and structure improved after infusion of BMMCs in the IR group (IR-C). Labeled BMMCs were found in the kidneys after therapy. The expression of inflammatory and biological markers (TLR-2, TRL-4, RAGE, IL-17, HMGB-1, KIM-1) were reduced and the expression of anti-inflammatory and antioxidant markers (IL-10, Nrf2, and HO-1) were increased in IR-C animals compared with IR untreated animals (IR-S). The apoptotic index diminished and the proliferation index increased in IR-C compared with IR-S.
  
  
  CONCLUSIONS
  The results contribute to our understanding of the role of different biological players in morphofunctional renal improvement and cytoprotection in a post-ischemic reperfusion kidney injury model subjected to cellular therapy.
authors.affiliation: Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.|Laboratory of Immunopathology, Rio de Janeiro, Brazil.
authors.forename: Felipe Mateus
authors.initials: FM
authors.lastname: Ornellas
authors.affiliation: Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
authors.forename: Débora Santos
authors.initials: DS
authors.lastname: Ornellas
authors.affiliation: Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
authors.forename: Sabrina Vargas
authors.initials: SV
authors.lastname: Martini
authors.affiliation: Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.|Laboratory for Clinical and Experimental Research on Vascular Biology, Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Raquel Carvalho
authors.initials: RC
authors.lastname: Castiglione
authors.affiliation: Confocal Microscopy Unit, Institute of Biomedical Science, Rio de Janeiro, Brazil.
authors.forename: Grasiella Maria
authors.initials: GM
authors.lastname: Ventura
authors.affiliation: Laboratory of Pulmonary Investigation, Institute of Biophysics Carlos Chagas Filho, Rio de Janeiro, Brazil.
authors.forename: Patrícia R
authors.initials: PR
authors.lastname: Rocco
authors.affiliation: Department of Radiology, School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Bianca
authors.initials: B
authors.lastname: Gutfilen
authors.affiliation: Department of Radiology, School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: de Souza
authors.affiliation: Laboratory of Immunopathology, Rio de Janeiro, Brazil.
authors.forename: Christina Maeda
authors.initials: CM
authors.lastname: Takiya
authors.affiliation: Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
authors.forename: Marcelo Marcos
authors.initials: MM
authors.lastname: Morales
mesh_terms: D064591:Allografts; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D016026:Bone Marrow Transplantation; D005260:Female; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D018836:Inflammation Mediators / Q000378:metabolism*; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; D018836:Inflammation Mediators
keywords: Bone marrow; Cellular therapy; Ischemia- reperfusion; Renal