title: Chemokine receptor CCR5 and CXCR4 might influence virus replication during IBDV infection.
abstract: Both CCR5 and CXCR4 are important chemokine receptors and take vital role in migration, development and distribution of T cells, however, whether they will influence the process of T cell infiltration into bursa of Fabricius during infectious bursal disease virus (IBDV) infection is unclear. In the current study, CCR5 and CXCR4 antagonists, Maraviroc and AMD3100, were administrated into chickens inoculated with IBDV, and the gene levels of IBDV VP2, CCR5, CXCR4 and related cytokines were determined by real-time PCR. The results showed that large number of T cells began to migrate into the bursae on Day 3 post infection with IBDV and the mRNA of chemokine receptors CCR5 and CXCR4 began to increase on Day 1. Moreover, antagonist treatments have increased the VP2, CCR5 and CXCR4 gene transcriptions and influenced on the gene levels of IL-2, IL-6, IL-8, IFN-γ, TGF-β4, MHC-I and MDA5. In conclusion, the chemokine receptors CCR5 and CXCR4 might influence virus replication during IBDV infection and further study would focus on the interaction between chemokine receptors and their ligands.
authors.affiliation: Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China; College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
authors.forename: Changbo
authors.initials: C
authors.lastname: Ou
authors.affiliation: Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China. Electronic address: xhkong@htu.cn.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Kong
authors.affiliation: Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China. Electronic address: lxingyou@sohu.com.
authors.forename: Xingyou
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001596:Benzylamines; D002060:Bursa of Fabricius / Q000821:virology; D065100:CCR5 Receptor Antagonists; D002465:Cell Movement; D002645:Chickens; D000080027:Cyclams; D003510:Cyclohexanes / Q000037:antagonists & inhibitors; D016207:Cytokines / Q000235:genetics; D006571:Heterocyclic Compounds / Q000037:antagonists & inhibitors; D007243:Infectious bursal disease virus / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D000077592:Maraviroc; D011201:Poultry Diseases / Q000821:virology; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics; D014230:Triazoles / Q000037:antagonists & inhibitors; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology*
chemical_list: D001596:Benzylamines; D065100:CCR5 Receptor Antagonists; D000080027:Cyclams; D003510:Cyclohexanes; D016207:Cytokines; D006571:Heterocyclic Compounds; D007376:Interleukin-2; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine; D016212:Transforming Growth Factor beta; D014230:Triazoles; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; C445082:transforming growth factor beta4; D007371:Interferon-gamma; D000077592:Maraviroc; C088327:plerixafor
keywords: CCR5; CXCR4; Infectious bursal disease virus; T cell migration