title: Plasma microRNA192 in combination with serum CA19-9 as non-invasive prognostic biomarker in periampullary carcinoma.
abstract: In this study, circulating microRNAs (miRNAs) are being investigated as non-invasive biomarkers for early diagnosis and prognosis of human cancers. Since the prognosis for pancreatobiliary subtype of periampullary carcinoma is poor, we assessed the prognostic relevance of miRNAs in combination with CA19-9 as noninvasive biomarker in periampullary carcinoma. Circulating miRNAs in plasma and serum CA19-9 were evaluated in periampullary carcinoma patients (n = 109) undergoing Whipple's pancreaticoduodenectomy and in healthy volunteers (n = 92). Tumour tissues were subjected to staging and subtyping prior to determining differentially expressed miRNAs in them by quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis involved correlation, receiver operating characteristic, logistic regression, survival analyses and Cox-proportional regression. Of the three differentially expressed circulating miRNA, miRNA192 was significantly increased both in circulation and in tumour tissue and correlated with tumour stage and aggressiveness (r = 0.96, P < 0.0001). Area under the curve of circulating miRNA192 + CA19-9 combination was 0.877 (95% confidence interval, 0.72 to 0.96) for stage III and 0.92 (95% confidence interval, 0.77 to 0.88) for tumour aggressiveness. The combination was associated with poor survival (median: 22 months, P = 0.0008) in stage III patients. Cox-proportional regression analysis revealed prognostic importance of combination of circulating miR192 and CA19-9 (HR = 1.005, P = 0.0001) in periampullary carcinoma. In conclusion, combination of circulating miRNA192 with serum CA19-9 is a better prognostic biomarker than CA19-9 alone.
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: K
authors.initials: K
authors.lastname: Murali Manohar
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Mitnala
authors.initials: M
authors.lastname: Sasikala
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Yesaswini
authors.initials: Y
authors.lastname: Kvsrr
authors.affiliation: 2 Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: V
authors.initials: V
authors.lastname: Sunil
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.|2 Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Rupjyoti
authors.initials: R
authors.lastname: Talukdar
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: Hvv
authors.initials: H
authors.lastname: Murthy
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: C
authors.initials: C
authors.lastname: Ramji
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.|2 Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: G V
authors.initials: GV
authors.lastname: Rao
authors.affiliation: 2 Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: R
authors.initials: R
authors.lastname: Pradeep
authors.affiliation: 1 Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.|2 Asian Institute of Gastroenterology, Hyderabad, India.
authors.forename: D Nageshwar
authors.initials: DN
authors.lastname: Reddy
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D018395:CA-19-9 Antigen / Q000097:blood*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000473:pathology; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; C546783:MIRN192 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA; biomarker; non-invasive; perimapullary carcinoma; prognosis