title: Anti-Salmonella activity and probiotic trends of Kluyveromyces marxianus S-2-05 and Kluyveromyces lactis S-3-05 isolated from a French cheese, Tomme d'Orchies.
abstract: Kluyveromyces marxianus S-2-05 and Kluyveromyces lactis S-3-05 were recently isolated from a traditional French cheese, Tomme d'Orchies, and characterized here for their advantages using a different application perspective. First, we established their anti-Salmonella activity and downregulation of the virulence sopD gene of Salmonella enterica subsp. enterica serovar Typhimurium, mainly in the presence of K. marxianus S-2-05. In addition to their antagonism, these non-Saccharomyces yeasts were able to survive under conditions mimicking the gastrointestinal environment and to form biofilms on an abiotic device such as polystyrene. These strains also displayed highly hydrophilic cell wall surfaces properties and capacity for adhesion to intestinal Caco-2 cells, thus enhancing their potential as probiotic strains.
authors.affiliation: Université Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Ceugniez
authors.affiliation: Université Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: Francoise.Coucheney@univ-lille1.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Coucheney
authors.affiliation: Université Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacques
authors.affiliation: Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, University of Liège, Liège, B-4000, Belgium.
authors.forename: Georges
authors.initials: G
authors.lastname: Daube
authors.affiliation: Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, University of Liège, Liège, B-4000, Belgium.
authors.forename: Véronique
authors.initials: V
authors.lastname: Delcenserie
authors.affiliation: Université Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: djamel.drider@univ-lille1.fr.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development; D018938:Caco-2 Cells; D002611:Cheese / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000382:microbiology; D006801:Humans; D007422:Intestines / Q000737:chemistry / Q000382:microbiology; D007716:Kluyveromyces / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D011137:Polystyrenes; D019936:Probiotics; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology*; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011137:Polystyrenes; C116105:SopD protein, Salmonella
keywords: Anti-Salmonella antagonism; Non-Saccharomyces yeasts; Probiotic