title: Recycling of carbon dioxide by free air CO2enriched (FACE) Bacillus sp. SS105 for enhanced production and optimization of biosurfactant.
abstract: Carbon dioxide utilizing bacterium Bacillus sp. SS105 was isolated from FACE (free air CO2enriched) sample. The strain was grown in shake flask containing minimal salt medium with 50mM NaHCO3as autotrophic carbon source and molasses as a low cost byproduct for mixotrophic growth. Carbon dioxide sequestration property of Bacillus sp. SS105 was determined by enzyme assay of carbonic anhydrase and ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO). Along with CO2sequestration this strain produced biosurfactant and its characterization by FTIR and1H NMR indicated lipopeptide nature. Optimization of process parameter along with nutrient sources for higher biosurfactant production was done by Response Surface Methodology (RSM). Under optimized conditions, the yield of biosurfactant and biomass was 2.65 and 2.78gL-1respectively. The study revealed simultaneous CO2sequestration and biosurfactant production by Bacillus sp. SS105.
authors.affiliation: Amity School of Earth and Environmental Science, Amity University Gurgaon, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Maheshwari
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Madan
authors.initials: M
authors.lastname: Kumar
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
authors.affiliation: Amity School of Earth and Environmental Science, Amity University Gurgaon, India; School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: shailisrivastava05@gmail.com.
authors.forename: Shaili
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D000388:Air; D001407:Bacillus; D002245:Carbon Dioxide / Q000378:metabolism*; D057965:Carbon Sequestration; D013501:Surface-Active Agents
chemical_list: D013501:Surface-Active Agents; D002245:Carbon Dioxide
keywords: Bacillus sp.; Biosurfactant; CO(2) sequestration; Free air CO(2) enrichment (FACE); Response Surface Methodology (RSM)