title: Urine metabolomics in neonates with late-onset sepsis in a case-control study.
abstract: Although late-onset sepsis (LOS) is a major cause of neonatal morbidity and mortality, biomarkers evaluated in LOS lack high diagnostic accuracy. In this prospective, case-control, pilot study, we aimed to determine the metabolic profile of neonates with LOS. Urine samples were collected at the day of initial LOS evaluation, the 3rdand 10thday, thereafter, from 16 septic neonates (9 confirmed and 7 possible LOS cases) and 16 non-septic ones (controls) at respective time points. Urine metabolic profiles were assessed using non-targeted nuclear magnetic resonance spectroscopy and targeted liquid chromatography-tandem mass spectrometry analysis. Multivariate statistical models with data from either analytical approach showed clear separation between the metabolic profiles of septic neonates (both possible and confirmed) and the controls. Metabolic changes appeared to be related to disease progression. Overall, neonates with confirmed or possible LOS exhibited comparable metabolic profiles indicating similar metabolic alternations upon the onset of clinical manifestations. This methodology therefore enabled the discrimination of neonates with LOS from non-septic individuals, providing potential for further research toward the discovery of LOS-related biomarkers.
authors.affiliation: 1st Department of Neonatology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Kosmas
authors.initials: K
authors.lastname: Sarafidis
authors.affiliation: School of Chemistry, Aristotle University of Thessaloniki, Greece.
authors.forename: Anastasia Chrysovalantou
authors.initials: AC
authors.lastname: Chatziioannou
authors.affiliation: 1st Department of Neonatology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Agathi
authors.initials: A
authors.lastname: Thomaidou
authors.affiliation: Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Helen
authors.initials: H
authors.lastname: Gika
authors.affiliation: School of Pharmacy, National and Kapodistrian University of Athens, Greece.
authors.forename: Emmanouel
authors.initials: E
authors.lastname: Mikros
authors.affiliation: School of Pharmacy, National and Kapodistrian University of Athens, Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Benaki
authors.affiliation: 1st Department of Neonatology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Elisavet
authors.initials: E
authors.lastname: Diamanti
authors.affiliation: 1st Department of Neonatology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Agakidis
authors.affiliation: Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Raikos
authors.affiliation: 1st Department of Neonatology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Drossou
authors.affiliation: School of Chemistry, Aristotle University of Thessaloniki, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Theodoridis
mesh_terms: D015415:Biomarkers / Q000652:urine*; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D006801:Humans; D007231:Infant, Newborn; D000067562:Late Onset Disorders / Q000175:diagnosis / Q000473:pathology*; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D000071074:Neonatal Sepsis / Q000175:diagnosis / Q000473:pathology*; D010865:Pilot Projects; D011446:Prospective Studies; D053719:Tandem Mass Spectrometry; D016482:Urinalysis; D014556:Urine / Q000737:chemistry*
chemical_list: D015415:Biomarkers