title: Application of DNA metabarcoding on faeces to identify European catfish Silurus glanis diet.
abstract: In this study, the results of conventional stomach-content analysis are compared with the recent DNA metabarcoding approach on faeces to identify fish species consumed by non-native European catfish Silurus glanis in the Garonne River (south-western France), with a special emphasis on anadromous prey. Fourteen prey species were identified in the stomach contents or faeces, including four anadromous fish species. Despite higher intestine than stomach emptiness, more species were identified through faecal analysis (11 of 14) than through stomach-content analysis (five of 14) suggesting that DNA metabarcoding on faeces is an efficient, non-intrusive technique to study the diet of predatory fishes.
authors.affiliation: EcoLab, Université de Toulouse, CNRS, INPT, UPS, 31062, Toulouse, France.|Station d'Ecologie Expérimental du CNRS à Moulis, Lab. USR 2936, 09 100, Moulis, France.
authors.forename: N
authors.initials: N
authors.lastname: Guillerault
authors.affiliation: EcoLab, Université de Toulouse, CNRS, INPT, UPS, 31062, Toulouse, France.
authors.forename: S
authors.initials: S
authors.lastname: Bouletreau
authors.affiliation: CNRS, UPS, ENFA, UMR5174 EDB (Laboratoire Évolution et Diversité Biologique), 31062, Toulouse, France.
authors.forename: A
authors.initials: A
authors.lastname: Iribar
authors.affiliation: SPYGEN, Savoie Technolac-Bât. Koala, 17, Rue du Lac Saint-André-BP 274, 73375, Le Bourget-du-Lac Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Valentini
authors.affiliation: EcoLab, Université de Toulouse, CNRS, INPT, UPS, 31062, Toulouse, France.
authors.forename: F
authors.initials: F
authors.lastname: Santoul
mesh_terms: D000818:Animals; D002397:Catfishes / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic / Q000662:veterinary*; D004032:Diet / Q000662:veterinary*; D005243:Feces / Q000737:chemistry*; D005504:Food Analysis; D005602:France
chemical_list: D004247:DNA
keywords: DNA metabarcoding; diet; excrement; predatory fish diet; stomach content analysis