title: Interaction of mycotoxin zearalenone with human serum albumin.
abstract: Zearalenone (ZEN) is a mycotoxin produced mainly by Fusarium species. Fungal contamination of cereals and plants can result in the formation of ZEN, leading to its presence in different foods, animal feeds, and drinks. Because ZEN is an endocrine disruptor, it causes reproductive disorders in farm animals and hyperoestrogenic syndromes in humans. Despite toxicokinetic properties of ZEN were studied in more species, we have no information regarding the interaction of ZEN with serum albumin. Since albumin commonly plays an important role in the toxicokinetics of different toxins, interaction of ZEN with albumin has of high biological importance. Therefore the interaction of ZEN with human serum albumin (HSA) was investigated using spectroscopic methods, ultrafiltration, and molecular modeling studies. Fluorescence spectroscopic studies demonstrate that ZEN forms complex with HSA. Binding constant (K) of ZEN-HSA complex was quantified with fluorescence quenching technique. The determined binding constant (logK=5.1) reflects the strong interaction of ZEN with albumin suggesting the potential biological importance of ZEN-HSA complex formation. Based on the results of the investigations with site markers as well as docking studies, ZEN occupies a non-conventional binding site on HSA. Considering the above listed observations, we should keep in mind this interaction if we would like to precisely understand the toxicokinetic behavior of ZEN.
authors.affiliation: Department of Pharmacology, University of Pécs, Faculty of Pharmacy, Szigeti út 12, Pécs H-7624, Hungary; Department of Pharmacology and Pharmacotherapy, University of Pécs, Medical School, Szigeti út 12, Pécs H-7624, Hungary. Electronic address: poor.miklos@pte.hu.
authors.forename: Miklós
authors.initials: M
authors.lastname: Poór
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6, Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20, Pécs H-7624, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Kunsági-Máté
authors.affiliation: Department of Pharmacology and Pharmacotherapy, University of Pécs, Medical School, Szigeti út 12, Pécs H-7624, Hungary; Department of Biochemistry, Eötvös Loránd University, Pázmány sétány 1/C, Budapest H-1117, Hungary.
authors.forename: Mónika
authors.initials: M
authors.lastname: Bálint
authors.affiliation: Department of Pharmacology and Pharmacotherapy, University of Pécs, Medical School, Szigeti út 12, Pécs H-7624, Hungary; MTA-ELTE Molecular Biophysics Research Group, Hungarian Academy of Sciences, Pázmány sétány 1/C, Budapest H-1117, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Hetényi
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6, Pécs H-7624, Hungary.
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Gerner
authors.affiliation: Department of General and Physical Chemistry, University of Pécs, Ifjúság útja 6, Pécs H-7624, Hungary; János Szentágothai Research Center, Ifjúság útja 20, Pécs H-7624, Hungary.
authors.forename: Beáta
authors.initials: B
authors.lastname: Lemli
mesh_terms: D001665:Binding Sites; D006801:Humans; D006860:Hydrogen Bonding; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D009183:Mycotoxins / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D013974:Thyroxine / Q000737:chemistry / Q000378:metabolism; D015025:Zearalenone / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009183:Mycotoxins; D012709:Serum Albumin; D015025:Zearalenone; D013974:Thyroxine
keywords: Albumin-ligand interaction; Fluorescence spectroscopy; Human serum albumin; Zearalenone