title: Phenotyping Multiple Subsets of Immune Cells In Situ in Formalin-Fixed, Paraffin-Embedded Tissue Sections.
abstract: Some somatic illnesses such as peripheral tumours can present with psychiatric symptoms. Many of these are characterized by changes in biomarkers related to the inflammation or immune response. Here, we describe a multispectral imaging protocol that can be used to phenotype immune and other cell types through simultaneous imaging of multiple proteins in sections of peripheral solid tumours and other tissues. This approach can also be used to assess the spatial organization of these cells within the tissue.
authors.affiliation: Andor Technology, 5 Millennium Way, Belfast, BT12 7AL, UK. jim@jmansfield.com.
authors.forename: James R
authors.initials: JR
authors.lastname: Mansfield
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP, Cidade Universitária Zeferino Vaz), Rua Monteiro Lobato 255 F/01, 13083-862, Campinas, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Jared
authors.initials: J
authors.lastname: Burks
mesh_terms: D015415:Biomarkers; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D005557:Formaldehyde; D006801:Humans; D007091:Image Processing, Computer-Assisted; D016130:Immunophenotyping / Q000379:methods*; D001523:Mental Disorders / Q000175:diagnosis / Q000276:immunology*; D008856:Microscopy, Fluorescence / Q000379:methods; D009363:Neoplasm Proteins / Q000032:analysis; D009369:Neoplasms / Q000175:diagnosis / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000032:analysis; D016612:Paraffin Embedding; D040901:Proteomics / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D005557:Formaldehyde
keywords: Biomarker; Immune cells; Multispectral imaging; Proteomics; Psychiatric disorders; Tumour