title: Identification of Key Candidate Genes and Pathways in Colorectal Cancer by Integrated Bioinformatical Analysis.
abstract: Colorectal cancer (CRC) is one of the most common malignant diseases worldwide, but the involved signaling pathways and driven-genes are largely unclear. This study integrated four cohorts profile datasets to elucidate the potential key candidate genes and pathways in CRC. Expression profiles GSE28000, GSE21815, GSE44076 and GSE75970, including 319 CRC and 103 normal mucosa, were integrated and deeply analyzed. Differentially expressed genes (DEGs) were sorted and candidate genes and pathways enrichment were analyzed. DEGs-associated protein-protein interaction network (PPI) was performed. Firstly, 292 shared DEGs (165 up-regulated and 127 down-regulated) were identified from the four GSE datasets. Secondly, the DEGs were clustered based on functions and signaling pathways with significant enrichment analysis. Thirdly, 180 nodes/DEGs were identified from DEGs PPI network complex. Lastly, the most significant 2 modules were filtered from PPI, 31 central node genes were identified and most of the corresponding genes are involved in cell cycle process, chemokines and G protein-coupled receptor signaling pathways. Taken above, using integrated bioinformatical analysis, we have identified DEGs candidate genes and pathways in CRC, which could improve our understanding of the cause and underlying molecular events, and these candidate genes and pathways could be therapeutic targets for CRC.
authors.affiliation: Institute of Precision Medicine, Jining Medical University, Jining 272067, China. guoyongchen2005@126.com.
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Precision Medicine, Jining Medical University, Jining 272067, China. baoyonghua2005@126.com.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Surgery, Jining Medical University, Jining 272067, China. mingma17@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Institute of Precision Medicine, Jining Medical University, Jining 272067, China. wyang06@uic.edu.|Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA. wyang06@uic.edu.
authors.forename: Wancai
authors.initials: W
authors.lastname: Yang
mesh_terms: D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D020022:Genetic Predisposition to Disease; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome
keywords: bioinformatical analysis; colorectal cancer; differentially expressed genes