title: Lipopolysaccharide of Pantoea agglomerans 7969: Chemical identification, function and biological activity.
abstract: Lipopolysaccharide (LPS) of Pantoea agglomerans 7969 isolated from apple tree was purified and characterized chemically by sugar and fatty acid analysis. Lipid A was analysed by negative-ion mode ESI MS and found to consist mainly of hexa- and tetra-acyl species typical of E. coli lipid A. The O-specific polysaccharide of the LPS was studied by sugar analysis, Smith degradation, and one- and two-dimensional1H and13C NMR spectroscopy. The polysaccharide is built up of linear tetrasaccharide repeating units, and about ∼25% repeats contain glycerol 1-phosphate on the GlcNAc residue: →3)-α-l-Rha p-(1→6)-α-d-Man p-(1→3)-α-d-Fuc p-(1→3)-β-d-Glc pNAc-(1→∼25% Gro-1-P-(O→6)⌋The LPS showed low levels of toxic and pyrogenic activities and reduced the average adhesion and the index of adhesiveness.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia. Electronic address: zdorovenko@mail.ru.
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Alexandra A
authors.initials: AA
authors.lastname: Kadykova
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: D.K. Zabolotny Insitute of Microbiology and Virology of the National Academy of Sciences, 154 Zabolotnoho Str., 03143 Kiev, Ukraine.
authors.forename: Ludmila D
authors.initials: LD
authors.lastname: Varbanets
authors.affiliation: D.K. Zabolotny Insitute of Microbiology and Virology of the National Academy of Sciences, 154 Zabolotnoho Str., 03143 Kiev, Ukraine.
authors.forename: Tetiana V
authors.initials: TV
authors.lastname: Bulyhina
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D027845:Malus / Q000382:microbiology; D019081:O Antigens; D020636:Pantoea / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens
keywords: Adhesiveness; Glycerol phosphate; Lipid A; O-Polysaccharide structure; Pantoea agglomerans; Pyrogenicity; Toxicity