title: Sub-cellular mRNA localization modulates the regulation of gene expression by small RNAs in bacteria.
abstract: Small non-coding RNAs can exert significant regulatory activity on gene expression in bacteria. In recent years, substantial progress has been made in understanding bacterial gene expression by sRNAs. However, recent findings that demonstrate that families of mRNAs show non-trivial sub-cellular distributions raise the question of how localization may affect the regulatory activity of sRNAs. Here we address this question within a simple mathematical model. We show that the non-uniform spatial distributions of mRNA can alter the threshold-linear response that characterizes sRNAs that act stoichiometrically, and modulate the hierarchy among targets co-regulated by the same sRNA. We also identify conditions where the sub-cellular organization of cofactors in the sRNA pathway can induce spatial heterogeneity on sRNA targets. Our results suggest that under certain conditions, interpretation and modeling of natural and synthetic gene regulatory circuits need to take into account the spatial organization of the transcripts of participating genes.
authors.affiliation: Department of Physics, Harvard University, Cambridge, MA 02138, United States of America.
authors.forename: Hamid
authors.initials: H
authors.lastname: Teimouri
authors.forename: Elgin
authors.initials: E
authors.lastname: Korkmazhan
authors.forename: Joel
authors.initials: J
authors.lastname: Stavans
authors.forename: Erel
authors.initials: E
authors.lastname: Levine
mesh_terms: D003198:Computer Simulation; D004843:Epistasis, Genetic; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D008954:Models, Biological; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D058727:RNA, Small Untranslated