title: Determination of the native forms of vitamin B1in bovine milk using a fast and simplified UHPLC method.
abstract: The aim of this study was to develop a high-throughput UHPLC method for the determination vitamin B1active compounds; thiamin, thiamin monophosphate and thiamin diphosphate in bovine milk. In order to sustain the native vitamin B1phosphorus esters, sample preparation is crucial. Various acids as well as commonly used enzymes and their enzyme mixtures were compared. Method accuracy was confirmed using certified reference material as well as comparison with the corresponding CEN method, and was found to be satisfactory. Studied milk samples showed significant amounts of thiamin monophosphate, which can make up to 53.9% of the total vitamin B1content in commercial milk, and up to 78% in raw milk. Moreover, a tremendous variation of the total content of vitamin B1was observed between single cows, which ranged from 0.24mg/L up to 0.54mg/L of total vitamin B1.
authors.affiliation: Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Vienna, Austria. Electronic address: anatol.schmidt@boku.ac.at.
authors.forename: Anatol
authors.initials: A
authors.lastname: Schmidt
authors.affiliation: Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Vienna, Austria.
authors.forename: Herbert
authors.initials: H
authors.lastname: Pratsch
authors.affiliation: Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Vienna, Austria.
authors.forename: Maximilian G
authors.initials: MG
authors.lastname: Schreiner
authors.affiliation: Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Vienna, Austria.
authors.forename: Helmut K
authors.initials: HK
authors.lastname: Mayer
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D013831:Thiamine / Q000032:analysis*
chemical_list: D013831:Thiamine
keywords: Milk; Thiamin; Thiamin (PubChem CID: 1130); Thiamin diphosphate (PubChem CID: 15938963); Thiamin phosphate (PubChem CID: 3382778); Thiamin phosphate esters; UHPLC