title: Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
abstract: We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
authors.forename: Zenpei
authors.initials: Z
authors.lastname: Shimatani
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kashojiya
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Takayama
authors.affiliation: Laboratory of Genetics and Breeding Science, Faculty of Agriculture, Meijo University, Nagoya, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Terada
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Arazoe
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
authors.forename: Hisaki
authors.initials: H
authors.lastname: Ishii
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Teramura
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Komatsu
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Miura
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
authors.forename: Keiji
authors.identifier: 0000-0002-9371-4971
authors.initials: K
authors.lastname: Nishida
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Ariizumi
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.|Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan.
authors.forename: Akihiko
authors.identifier: 0000-0003-1527-5288
authors.initials: A
authors.lastname: Kondo
mesh_terms: D020029:Base Pairing / Q000235:genetics; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D003564:Cytidine Deaminase / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D017343:Genes, Plant / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D017354:Point Mutation / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D064130:CRISPR-Associated Proteins; D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D003564:Cytidine Deaminase