title: Targeting effect of microRNA on CD133 and its impact analysis on proliferation and invasion of glioma cells.
abstract: MiR-200b, a member of the microRNA-200 family, has been identified to be capable of suppressing glioma cell growth through targeting CREB1 or CD133. However, whether miR-200b affects the biological behavior (proliferation, invasion, and migration) of glioma cells is poorly understood. The aim of this study was to evaluate the effect of miR-200b on the biological behavior of glioma cells in vitro. MiRNA-200b mimics, miRNA-200b inhibitor, and mimic control were transfected into conventionally cultured glioma U251 cells, followed by measuring the expression of miR-200b and CD133 in transfected cells by RT-PCR; effect of miR-200b on CD133 mRNA 3'-UTR luciferase activity by luciferase reporter assay; proliferation activity of transfected U251 cells by MTT method; and changes in U251 cell invasion and migration by Transwell method after transfection. Compared to that in the miRNA-200b inhibitor, mimic control, and blank control groups, miRNA-200b expression was significantly increased and CD133 mRNA expression was significantly decreased in the mimic miRNA-200b group in a time-dependent manner (P < 0.05). Meanwhile, dual luciferase reporter assay showed that miR-200b could inhibit CD133 activity through binding to the 3'-UTR of CD133 mRNA (P < 0.05). Furthermore, the proliferation activity and invasion and migration abilities of U251 cells transfected with miRNA-200b mimic were significantly decreased (P < 0.05). In conclusion, overexpression of miR-200b inhibited the proliferation, invasion, and migration of glioma cells possibly through targeting CD133.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.|Department of Neurosurgery, The People's Hospital of Rizhao, Rizhao, Shandong, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Fifth Hospital of PLA, Yinchuan, Ningxia, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Ma
authors.affiliation: Department of Laboratory, The People's Hospital of Rizhao, Rizhao, Shandong, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Mou
authors.affiliation: Department of Neurosurgery, The People's Hospital of Rizhao, Rizhao, Shandong, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The People's Hospital of Rizhao, Rizhao, Shandong, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China guangmingxu220@sina.com.
authors.forename: W C
authors.initials: WC
authors.lastname: Yao
mesh_terms: D020413:3' Untranslated Regions; D000071916:AC133 Antigen / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D009423:Nervous System Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D000071916:AC133 Antigen; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; C000606023:PROM1 protein, human; D012333:RNA, Messenger