title: Xyloglucan, hibiscus and propolis for the prevention of urinary tract infections: results of in vitro studies.
abstract: To assess the properties of a medical device containing xyloglucan, propolis and hibiscus to create a bioprotective barrier to avoid the contact of uropathogenic Escherichia coli strains on cell walls in models of intestinal (CacoGoblet) and uroepithelial (RWPE-1) cells (derived from normal human prostate epithelium).
  
  
  
  Two uropathogenic E. coli strains (expressing type 1 fimbriae and P fimbriae) were used to assess, by electronic microscopy and ELISA, the barrier properties of the medical device. The antimicrobial activity was assessed in broth dilution assays.
  
  
  
  The three components (xyloglucan, propolis and hibiscus) did not alter E. coli cell integrity in intestinal and uroepithelial cell models and were devoid of antibacterial activity. The three components avoided bacterial contact in both cell monolayers.
  
  
  
  The nonpharmacological barrier properties of xyloglucan, propolis and hibiscus confirm the role of the medical device for the management of urinary tract infections.
authors.affiliation: Department of Biomedicine & Biotechnology, University of Alcalá, 28871, Alcalá de Henares, Madrid, Spain.
authors.forename: Benito
authors.initials: B
authors.lastname: Fraile
authors.affiliation: Laboratorios DIATER, SA. Avenida Gregorio Peces Barba, no. 2. Parque Tecnológico de Leganés. 208918 Leganés, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Alcover
authors.affiliation: Department of Biomedicine & Biotechnology, University of Alcalá, 28871, Alcalá de Henares, Madrid, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Royuela
authors.affiliation: Laboratorios DIATER, SA. Avenida Gregorio Peces Barba, no. 2. Parque Tecnológico de Leganés. 208918 Leganés, Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Rodríguez
authors.affiliation: Department of Biomedicine & Biotechnology, University of Alcalá, 28871, Alcalá de Henares, Madrid, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Chaves
authors.affiliation: Laboratorios DIATER, SA. Avenida Gregorio Peces Barba, no. 2. Parque Tecnológico de Leganés. 208918 Leganés, Madrid, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Palacios
authors.affiliation: Department of Microbiology & Parasitology, Pharmacy Faculty, Universitat de Barcelona (UB), Diagonal Sud, Facultat de Farmàcia, Edifici A, Av Joan XXIII, 08028 Barcelona, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Piqué
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D056705:Apitherapy; D001422:Bacterial Adhesion / Q000187:drug effects; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control; D010861:Fimbriae, Bacterial / Q000187:drug effects; D005936:Glucans / Q000494:pharmacology*; D031584:Hibiscus; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D011429:Propolis / Q000494:pharmacology*; D014552:Urinary Tract Infections / Q000382:microbiology / Q000517:prevention & control*; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000502:physiology; D014990:Xylans / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D011429:Propolis
keywords: hibiscus; intestinal epithelial cells; medical device; propolis; urinary tract infection; xyloglucan