title: A Protocol for Generation of a Corticosterone Model of Psychiatric Disorders.
abstract: Some patients with psychiatric disorders show hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis. This may be due to an impaired feedback inhibition and can be seen through increased levels of circulating cortisol. Here a protocol is described to mimic this situation by subcutaneous implantation of corticosterone pellets in mice. We also present characterization of the model by looking at effects on neuronal proliferation in hippocampus, one of the main tissues known to be affected by HPA axis hyper-activation. Such tissues could be used in analyses by proteomic platforms.
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil. paulcguest@yahoo.com.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D000818:Animals; D003345:Corticosterone / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D004195:Disease Models, Animal; D004343:Drug Implants; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006624:Hippocampus / Q000737:chemistry / Q000187:drug effects*; D001523:Mental Disorders / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D040901:Proteomics / Q000379:methods*; D011965:Receptors, Glucocorticoid / Q000819:agonists / Q000502:physiology; D018161:Receptors, Mineralocorticoid / Q000819:agonists / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D004343:Drug Implants; D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D003345:Corticosterone
keywords: Cell proliferation; Corticosteroid; HPA axis; Hippocampus; Proteomics; Psychiatric disorders