title: Kinetics of α-glucosidase inhibition by different fractions of three species of Labiatae extracts: a new diabetes treatment model.
abstract: BACKGROUND
  Glucosidases are a group of enzymes playing crucial roles in digestion of carbohydrates. Glucosidase inhibitors can reduce carbohydrate digestion rate and have the potential to prevent development of type 2 diabetes. The Labiatae is one of the largest plant families grown globally and many studies that have isolated new pharmaceutical compounds. In folk medicine, some of Labiatae plants such as Zataria multiflora Boiss, Salvia mirzayanii Rech. F. & Esfand, and Otostegia persica Boiss are consumed for the treatment of diabetes.
  
  
  OBJECTIVE
  This study investigates the inhibitory effects of different fractions of three mentioned species extracts on α-glucosidase.
  
  
  METHODS
  Ethanol extracts of these plants leaves were fractionated using petroleum ether, chloroform, ethyl acetate, and n-butanol solutions. The duration of this study was 12 months. To measure enzyme inhibition, 5 μL of the enzyme, 20 μL of substrate and samples were used and for evaluation mode of inhibition, constant amounts of α-glucosidase were incubated with rising concentrations of substrate (PNPG).
  
  
  RESULTS
  The results revealed that the ethyl acetate fraction of Zataria multiflora (IC50= 0.35 ± 0.01 mg/mL) and petroleum ether fraction of Salvia mirzayanii (IC50= 0.4 ± 0.11 mg/mL) were the most potent inhibitors of α-glucosidase in comparison with the other samples and acarbose as the standard (IC50= 7 ± 0.19 mg/mL). All of the samples exhibited noncompetitive-uncompetitive inhibition.
  
  
  CONCLUSIONS
  It can be inferred from this study that α-glucosidase inhibitory potential of the studied extracts may be a marker of antidiabetic potential of these extracts.
authors.affiliation: a Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences , Bandar Abbas , IR Iran.|b Biochemistry Department, Faculty of Medicine , Hormozgan University of Medical Sciences , Bandar Abbas , IR Iran.
authors.forename: Sahere
authors.initials: S
authors.lastname: Rouzbehan
authors.affiliation: a Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences , Bandar Abbas , IR Iran.|b Biochemistry Department, Faculty of Medicine , Hormozgan University of Medical Sciences , Bandar Abbas , IR Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Moein
authors.affiliation: c Department of Biochemistry, Faculty of Science , University of Hormozgan , Bandar Abbas , IR Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Homaei
authors.affiliation: d Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences , Shiraz , IR Iran.|e Department of Pharmacognosy , School of Pharmacy, Shiraz University of Medical Sciences , Shiraz , IR Iran.
authors.forename: Mahmood Reza
authors.initials: MR
authors.lastname: Moein
mesh_terms: D020909:Acarbose / Q000494:pharmacology; D000085:Acetates / Q000737:chemistry; D000473:Alkanes / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D065089:Glycoside Hydrolase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D019686:Lamiaceae / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D000085:Acetates; D000473:Alkanes; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D012997:Solvents; C007650:ethyl acetate; D000520:alpha-Glucosidases; C004544:naphtha; D020909:Acarbose
keywords: Enzyme retardation; Otostegia persica; Salvia mirzayanii; Zataria multifolra