title: Predictive value of p63, ki-67, and survivin expression in oral leukoplakia: A tissue microarray study.
abstract: The aim of this study was to analyze the immunohistochemical expression of survivin, ki-67, and p63 in oral leukoplakic lesions, histopathologically differentiated into dysplastic and nondysplastic. A tissue microarray containing 57 samples of biopsies from clinically classified lesions, such as leukoplakia, was immunolabeled for survivin, ki-67, and p63. Samples were scored for percentage of positively stained. Scores were designated as follows: low = less than 25% of positive cells; and high = more than 25% of positive cells. On performing histopathological diagnosis, 20 dysplastic lesions and 37 nondysplastic lesions were seen, in which female patients (56.1%) were predominant with an average age of 58.27 years. The study showed a high expression of 37.5% for survivin, 43.7% for ki-67, and 88.2% for p63 in dysplastic lesions. However, there was a high expression of 16.7% for survivin, 16.7% for ki-67, and 92% for p63 in nondysplastic lesions. There is a positive correlation of expression among the three antibodies. In the association of immunoreactivity, in both dysplastic and nondysplastic lesions, increased expression of survivin reflects on the increased expression of ki-67, and there is an overexpression of p63. In leukoplakia, the expression of survivin associated with that of ki-67 reinforces the assumption that all these lesions are potentially malignant, regardless of histopathology; and the overexpression of p63 may indicate carcinogenic potential. These findings may help in the treatment of patients with this type of lesion.
authors.affiliation: Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Kelly
authors.initials: K
authors.lastname: Bienk Dias
authors.affiliation: Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Anacláudia
authors.initials: A
authors.lastname: Pereira Costa Flores
authors.affiliation: Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Márcia
authors.identifier: http://orcid.org/0000-0002-6037-4456
authors.initials: M
authors.lastname: Gaiger Oliveira
authors.affiliation: Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Pantelis
authors.initials: P
authors.lastname: Varvaki Rados
authors.affiliation: Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Manoel
authors.initials: M
authors.lastname: Sant'ana Filho
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism*; D007972:Leukoplakia, Oral / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000077022:Survivin; D046888:Tissue Array Analysis
chemical_list: C107489:BIRC5 protein, human; C081876:CKAP4 protein, human; D051034:Inhibitor of Apoptosis Proteins; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D000077022:Survivin
keywords: Immunohistochemistry; leukoplakia; oral; tissue microarray