title: circRNA_100290 plays a role in oral cancer by functioning as a sponge of the miR-29 family.
abstract: Circular RNAs (circRNAs) represent a class of non-coding RNAs that are widely expressed in mammals. However, it is largely unknown about the function of human circRNAs and the roles of circRNAs in human oral squamous cell carcinomas (OSCC). Here we performed a comprehensive study of circRNAs in human OSCC using circRNA and mRNA microarrays, and identified many circRNAs that are differentially expressed between OSCC tissue and paired non-cancerous matched tissue. We further found a circRNA termed circRNA_100290 that served as a critical regulator in OSCC development. We discovered that circRNA_100290 was upregulated and co-expressed with CDK6 in OSCC tissue. Knockdown of circRNA_100290 decreased expression of CDK6 and inhibited proliferation of OSCC cell lines in vitro and in vivo. Via luciferase reporter assays, circRNA_100290 was observed to directly bind to miR-29 family members. Further EGFP/RFP reporter assays showed that CDK6 was the direct target of miR-29b. Taken together, we conclude that circRNA_100290 may function as a competing endogenous RNA to regulate CDK6 expression through sponging up miR-29b family members. Taken together, it indicates that circRNAs may exert regulatory functions in OSCC and may be a potential target for OSCC therapy.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, P.R. China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, People's Hospital of New District Longhua Shenzhen, Shenzhen, P.R. China.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, P.R. China.
authors.forename: J
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, P.R. China.
authors.forename: S
authors.initials: S
authors.lastname: Ge
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D051361:Cyclin-Dependent Kinase 6 / Q000378:metabolism; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D000079962:RNA, Circular; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D046888:Tissue Array Analysis; D015854:Up-Regulation
chemical_list: D027682:Cation Transport Proteins; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D000079962:RNA, Circular; D012333:RNA, Messenger; C472294:SLC30A7 protein, human; D012313:RNA; D008156:Luciferases; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6