title: d-Tagatose production by permeabilized and immobilized Lactobacillus plantarum using whey permeate.
abstract: The aim of the work is to produce d-Tagatose by direct addition of alginate immobilized Lactobacillus plantarum cells to lactose hydrolysed whey permeate. The cells were untreated and immobilized (UIC), permeabilized and immobilized (PIC) and the relative activities were compared with purified l-arabinose isomerase (l-AI) for d-galactose isomerization. Successive lactose hydrolysis by β-galactosidase from Escherichia coli and d-galactose isomerization using l-AI from Lactobacillus plantarum was performed to investigate the in vivo production of d-tagatose in whey permeate. In whey permeate, maximum conversion of 38% and 33% (w/w) d-galactose isomerization by PIC and UIC has been obtained. 162mg/g and 141mg/g of d-tagatose production was recorded in a 48h reaction time at 50°C, pH 7.0 with 5mM Mn2+ion concentration in the initial substrate mixture.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, Tamilnadu, India. Electronic address: jjnagai81@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Jayamuthunagai
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, Tamilnadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Srisowmeya
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Chakravarthy
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, Tamilnadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Gautam
mesh_terms: D019747:Aldose-Ketose Isomerases; D005690:Galactose / Q000737:chemistry; D006601:Hexoses / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum; D000067796:Whey; D001616:beta-Galactosidase
chemical_list: D006601:Hexoses; D001616:beta-Galactosidase; D019747:Aldose-Ketose Isomerases; D005690:Galactose
keywords: Lactose; Whey permeate; d-Galactose; d-Tagatose; l-Arabinose isomerase