title: Understanding Protein Structure Deformation on the Surface of Gold Nanoparticles of Varying Size.
abstract: Gold nanoparticles (AuNPs) have been of recent interest due to their unique optical properties and their biocompatibility. Biomolecules spontaneously adsorb to their surface, a trait that could potentially be exploited for drug targeting. Currently, it is unclear whether protein-AuNP interactions at the nanoparticle surface are dependent on nanoparticle size. In this work, we investigate whether varying surface curvature can induce protein unfolding and multilayer binding in citrate-coated AuNPs of various sizes. A recently developed NMR-based approach was utilized to determine the adsorption capacity, and protein NMR spectra were compared to determine whether nanoparticle size influences protein interactions at the surface. In addition, transmission electron microscopy (TEM) and dynamic light scattering (DLS) were employed to corroborate the NMR studies. Over a broad range of AuNP sizes (14-86 nm), we show that adsorption capacity can be predicted by assuming that proteins are compact and globular on the nanoparticle surface. Additionally, roughly one layer of proteins is adsorbed regardless of AuNP size. Our results hold for two proteins of significantly different sizes, GB3 (6 kDa) and bovine carbonic anhydrase (BCA, 29 kDa). However, the unstable drkN SH3 domain (ΔḠ0≈ 0, 7 kDa) does not appear to follow the same trend seen for stable, globular proteins. This observation suggests that unstable proteins can deform significantly when bound to AuNP surfaces. Taken together, the results of this work can be used to improve our knowledge of the mechanism of protein-AuNP interactions to optimize their use in the biomedical field.
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Woods
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Y Randika
authors.initials: YR
authors.lastname: Perera
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Mackenzie B
authors.initials: MB
authors.lastname: Davidson
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Chloe A
authors.initials: CA
authors.lastname: Wilks
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Yadav
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Fitzkee