title: Exploring Human Antimicrobial Antibody Responses on a Single B Cell Level.
abstract: Analysis of monoclonal antibodies (MAbs) derived from single B cell cloning has been highly beneficial for antimicrobial immunotherapy, vaccine design, and advancing our understanding of pathogen-triggered effects on the human immunoglobulin repertoire. Sequencing of variable domains of single B cells, and characterization of binding and functional activities of MAbs derived from those sequences, provides in-depth insight not only into sites of susceptibility for antibody-mediated neutralization or opsonization of the pathogen but also into the dynamics of protective antibody evolution during infection. This information can be utilized to rapidly develop novel immunotherapies of completely human origin and provides a roadmap for structure-based vaccine design that aims to elicit similar protective antibody responses. Here, we summarize recent aspects of the single B cell cloning approach.
authors.affiliation: Evotec AG, Manfred Eigen Campus, Hamburg, Germany daniel.hofmann@evotec.com jon.lai@einstein.yu.edu.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hofmann
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA daniel.hofmann@evotec.com jon.lai@einstein.yu.edu.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Lai
mesh_terms: D000890:Anti-Infective Agents / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D006801:Humans
chemical_list: D000890:Anti-Infective Agents; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing
keywords: antibody discovery; immune responses; immunotherapy; single B cell sorting