title: The effects of Nobiletin, Hesperetin, and Letrozole in a combination on the activity and expression of aromatase in breast cancer cells.
abstract: Nobiletin (NOB) and hesperetin (HES) are the citrus polymethoxyflavone and flavonone. Aromatase or cytochrome P450 (CYP19) enzyme is a key enzyme in estrogen biosynthesis. The objective of this study was to investigate the combinational effects of HES, NOB and letrozole (LET) as aromatase inhibitors on the activity and expression of aromatase in MCF-7 cells. In this study, aromatase enzyme activity based on the conversion of androgen substrate testosterone to 17β-Estradiol was determined. Estradiol concentrations were measured using an electrochemiluminescence immunoassay. CYP19 gene expression was determined by quantitative real-time PCR. Our findings demonstrated that none of combinations including LET+NOB, LET+HES, LET+NOB+HES, and NOB+HES had no significant effects on aromatase activity and expression. The present study showed for the first time that the combination of HES, NOB, and LET had no effects on activity and expression of aromatase in MCF-7 breast cancer cells.
authors.affiliation: Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: S T
authors.initials: ST
authors.lastname: Rahideh
authors.affiliation: Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Keramatipour
authors.affiliation: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Nourbakhsh
authors.affiliation: Department of Molecular and Cellular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Koohdani
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hoseini
authors.affiliation: Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Shidfar
mesh_terms: D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D047072:Aromatase Inhibitors / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism; D047309:Flavones / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D000077289:Letrozole; D061986:MCF-7 Cells; D009570:Nitriles / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D047072:Aromatase Inhibitors; D047309:Flavones; D009570:Nitriles; D014230:Triazoles; D004958:Estradiol; D000077289:Letrozole; C008661:nobiletin; D006569:Hesperidin; D001141:Aromatase; C561410:CYP19A1 protein, human; C013015:hesperetin
keywords: Aromatase; Breast cancer; Hesperetin; Letrozole; MCF-7 cells.; Nobiletin