title: Analysis of High-Throughput RNA Bisulfite Sequencing Data.
abstract: Methylation of the 5-cytosine (m5C) is a common but not well-understood RNA modification, which can be detected by sequencing of bisulfite-treated transcripts (RNA-BSseq). In this Chapter, we discuss computational RNA-BSseq data analysis methods for transcriptome-wide identification and quantification of m5C.
authors.affiliation: Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Innrain 80/IV, Innsbruck, 6020, Austria. dietmar.rieder@i-med.ac.at.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Rieder
authors.affiliation: Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Innrain 80/IV, Innsbruck, 6020, Austria.
authors.forename: Francesca
authors.initials: F
authors.lastname: Finotello
mesh_terms: D044503:5-Methylcytosine; D001482:Base Composition; D019295:Computational Biology / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D008745:Methylation; D011786:Quality Control; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D012984:Software; D059467:Transcriptome
chemical_list: D012313:RNA; D044503:5-Methylcytosine
keywords: (Cytosine-5) methylation; 5-methylcytosine; Bisulfite conversion; Data analysis; Differential methylation; High-throughput sequencing; Modification; RNA; RNA methylation; RNA modification; RNA-BSseq; Sodium bisulfite; Transcription; m5C