title: Adiponectin: Serum-saliva associations and relations with oral and systemic markers of inflammation.
abstract: This study addresses gaps in our understanding about the validity and utility of using salivary adiponectin to index serum adiponectin levels. Matched blood and saliva samples were collected on a single occasion from healthy adults (n=99; age 18-36 years, 53% male). Serum and saliva was assayed for adiponectin and inflammatory cytokines (IL-1β, IL-6, IL-8, TNFα), and saliva was also assayed for markers of blood contamination (transferrin), total protein (salivary flow rate) and matrix metalloproteinase-8 (MMP-8). We examined the extent to which salivary adiponectin was associated with serum adiponectin, and the influence of potential confounders on the serum-saliva correlation, including age, sex, body mass index, and markers of inflammation, oral health, salivary blood contamination, and flow rate. Findings revealed a modest serum-saliva association for adiponectin, and strong positive associations between salivary adiponectin and salivary levels of inflammatory cytokines, MMP-8, transferrin, and total protein. By contrast, salivary adiponectin was not related to serum levels of inflammatory activity. The magnitude of the serum-saliva association was strengthened when controlling for total protein in saliva, blood leakage into oral fluid, salivary inflammatory cytokines, and MMP-8. The pattern of findings extends our understanding of salivary adiponectin and its potential use as an index of circulating adiponectin levels.
authors.affiliation: Department of Population, Family, and Reproductive Health, Johns Hopkins University Bloomberg School of Public Health, MD, USA; Institute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA. Electronic address: jriis1@jhu.edu.
authors.forename: Jenna L
authors.initials: JL
authors.lastname: Riis
authors.affiliation: Institute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA; Sanford School of Social and Family Dynamics, Arizona State University, Tempe, AZ, USA.
authors.forename: Crystal I
authors.initials: CI
authors.lastname: Bryce
authors.affiliation: Institute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA; Department of Psychology, Arizona State University, Tempe, AZ, USA.
authors.forename: Thao
authors.initials: T
authors.lastname: Ha
authors.affiliation: Institute for Genomic Medicine, Johns Hopkins University School of Medicine, MD, USA.
authors.forename: Tracey
authors.initials: T
authors.lastname: Hand
authors.affiliation: Salimetrics Research and Technology Center, Carlsbad, CA, USA.
authors.forename: John L
authors.initials: JL
authors.lastname: Stebbins
authors.affiliation: Salimetrics Research and Technology Center, Carlsbad, CA, USA.
authors.forename: Marla
authors.initials: M
authors.lastname: Matin
authors.affiliation: School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK.
authors.forename: Katrin M
authors.initials: KM
authors.lastname: Jaedicke
authors.affiliation: Department of Population, Family, and Reproductive Health, Johns Hopkins University Bloomberg School of Public Health, MD, USA; Institute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA; Department of Acute and Chronic Care, Johns Hopkins University School of Nursing, MD, USA; Department of Pediatrics, Johns Hopkins University School of Medicine, MD, USA; Salimetrics Research and Technology Center, Carlsbad, CA, USA; Salivary Bioscience Laboratory and Department of Psychology, University of Nebraska, Lincoln, USA. Electronic address: IISBR@uci.edu.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Granger
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D015992:Body Mass Index; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000032:analysis; D007249:Inflammation / Q000276:immunology*; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D009909:Oral Health; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D044967:Serum / Q000737:chemistry*; D012737:Sex Factors; D014168:Transferrin / Q000032:analysis
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D015415:Biomarkers; D016207:Cytokines; D007155:Immunologic Factors; D012471:Salivary Proteins and Peptides; D014168:Transferrin; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8
keywords: Adiponectin; Cytokines; Inflammation; Oral health; Saliva; Serum