title: A quantitative method for the determination of bosutinib in human plasma using high-performance liquid chromatography and ultraviolet detection.
abstract: BACKGROUND
  We propose a simple, sensitive, and fast high-performance liquid chromatography ultraviolet detection (HPLC-UV) method for the quantitative determination of bosutinib in human plasma.
  
  
  METHODS
  Plasma samples were processed using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Bosutinib and the internal standard imatinib were separated using a mobile phase of 0.5% Na2PO4H2O (pH 3.5)-acetonitrile-methanol (55:25:20, v/v/v) on a CAPCELL PAK C18 MG II reversed-phase column 250 nm×4.6 nm i.d., at a flow rate of 1.0 mL/min, with ultraviolet detection at 250 nm.
  
  
  RESULTS
  The calibration curve exhibited linearity over the bosutinib concentration range of 25-1500 ng/mL at 250 nm, with coefficient of variation for intraday precision of 2.42%, 6.04%, and 1.11% for 100, 250, and 1500 ng/mL, respectively, of bosutinib. The lower limit of detection was 20 ng/mL. The extraction recovery rates for bosutinib ranged from 84.36% to 85.82%. The intra- and interday precision was below 8.7%, and the accuracy ranged from -5.95% to 5.85% over the linear range. No notable matrix effects or astaticism were observed.
  
  
  CONCLUSIONS
  The proposed HPLC-UV method was successfully applied as an assay to detect bosutinib in human plasma.
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan.
authors.forename: Takahiro
authors.identifier: http://orcid.org/0000-0002-2026-2142
authors.initials: T
authors.lastname: Sumimoto
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakahara
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Itoh
mesh_terms: D000814:Aniline Compounds / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D016014:Linear Models; D009570:Nitriles / Q000097:blood*; D011804:Quinolines / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D000814:Aniline Compounds; D009570:Nitriles; D011804:Quinolines; C471992:bosutinib
keywords: assay; bosutinib; chromic myeloid leukemia; high-performance liquid chromatography