title: Antioxidant capacity of phenolic compounds on human cell lines as affected by grape-tyrosinase and Botrytis-laccase oxidation.
abstract: Phenolic components (PCs) are well-known for their positive impact on human health. In addition to their action as radical scavengers, they act as activators for the intrinsic cellular antioxidant system. Polyphenol oxidases (PPOs) such as tyrosinase and laccase catalyze the enzymatic oxidation of PCs and thus, can alter their scavenging and antioxidative capacity. In this study, oxidation by tryosinase was shown to increase the antioxidant capacity of many PCs, especially those that lack adjacent aromatic hydroxyl groups. In contrast, oxidation by laccase tended to decrease the antioxidant capacity of red wine and distinct PCs. This was clearly demonstrated for p-coumaric acid and resveratrol, which is associated with many health benefits. While oxidation by tyrosinase increased their antioxidant activity laccase treatment resulted in a decreased activity and also of that for red wines.
authors.affiliation: Institute for Molecular Biophysics, Johannes Gutenberg-University Mainz, Jakob-Welder Weg 26, 55128 Mainz, Germany. Electronic address: riebelm@uni-mainz.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Riebel
authors.affiliation: Institute for Microbiology and Wine Research, Johannes Gutenberg-University Mainz, Johann-Joachim-Becherweg 15, 55128 Mainz, Germany. Electronic address: andrea-sabel@gmx.de.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sabel
authors.affiliation: Institute for Microbiology and Wine Research, Johannes Gutenberg-University Mainz, Johann-Joachim-Becherweg 15, 55128 Mainz, Germany. Electronic address: hclaus@uni-mainz.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Claus
authors.affiliation: Department of Pharmacology, Johannes Gutenberg University Medical Center Mainz, Obere Zahlbacher Str. 67, 55131 Mainz, Germany. Electronic address: xianing@uni-mainz.de.
authors.forename: Ning
authors.initials: N
authors.lastname: Xia
authors.affiliation: Department of Pharmacology, Johannes Gutenberg University Medical Center Mainz, Obere Zahlbacher Str. 67, 55131 Mainz, Germany. Electronic address: huigeli@uni-mainz.de.
authors.forename: Huige
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute for Microbiology and Wine Research, Johannes Gutenberg-University Mainz, Johann-Joachim-Becherweg 15, 55128 Mainz, Germany. Electronic address: hkoenig@uni-mainz.de.
authors.forename: Helmut
authors.initials: H
authors.lastname: König
authors.affiliation: Institute for Molecular Biophysics, Johannes Gutenberg-University Mainz, Jakob-Welder Weg 26, 55128 Mainz, Germany. Electronic address: hdecker@uni-mainz.de.
authors.forename: Heinz
authors.initials: H
authors.lastname: Decker
authors.affiliation: Institute for Molecular Biophysics, Johannes Gutenberg-University Mainz, Jakob-Welder Weg 26, 55128 Mainz, Germany. Electronic address: petra.fronk@uni-mainz.de.
authors.forename: Petra
authors.initials: P
authors.lastname: Fronk
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D020171:Botrytis / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D042845:Laccase / Q000032:analysis / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry / Q000378:metabolism*; D014920:Wine / Q000032:analysis
chemical_list: D000975:Antioxidants; D010636:Phenols; D042845:Laccase; D014442:Monophenol Monooxygenase
keywords: Antioxidant capacity; Laccase; Phenolic components; Radical scavenging; Tyrosinase; Wine