title: Plasma coenzyme Q10concentration, antioxidant status, and serum N-terminal pro-brain natriuretic peptide concentration in dogs with various cardiovascular diseases and the effect of cardiac treatment on measured variables.
abstract: OBJECTIVE To determine the plasma total antioxidant capacity, erythrocyte superoxide dismutase activity, whole blood glutathione peroxidase activity, and plasma coenzyme Q10(CoQ10) concentration in dogs with various stages of cardiovascular diseases and in healthy dogs; assess the influence of cardiac treatment on the levels of antioxidant variables, plasma CoQ10concentration, and serum N-terminal pro-brain natriuretic peptide (NT-proBNP) concentration, and determine any correlation between the disease severity (NT-proBNP concentration) and antioxidant variables or CoQ10concentration. ANIMALS 43 dogs with various types and stages of cardiovascular diseases (congenital and acquired) and 29 healthy dogs. PROCEDURES Blood samples were collected from all dogs for spectrophotometric assessment of antioxidant variables. Plasma CoQ10concentration was determined with a high-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry method. Serum NT-proBNP concentration was measured with an ELISA. RESULTS Values for antioxidant variables did not differ among groups of dogs with cardiovascular diseases, regardless of disease stage or treatment. Plasma CoQ10concentration was significantly increased in treated dogs with congestive heart failure (CHF), compared with untreated patients. However, plasma CoQ10concentration did not differ among heart failure classes. A significant, negative correlation between serum NT-proBNP and plasma CoQ10concentrations was identified in treated CHF-affected dogs, suggesting that low plasma CoQ10concentration may be associated with increased severity of CHF. CONCLUSIONS AND CLINICAL RELEVANCE The antioxidant variables evaluated were not altered in dogs with CHF, regardless of cardiac disease stage or treatment. Further investigation into the possible effects of CoQ10supplementation in dogs with advanced stages of CHF is warranted.
authors.forename: Alenka Nemec
authors.initials: AN
authors.lastname: Svete
authors.forename: Barbara
authors.initials: B
authors.lastname: Verk
authors.forename: Alenka
authors.initials: A
authors.lastname: Seliškar
authors.forename: Katerina
authors.initials: K
authors.lastname: Tomsič
authors.forename: Petra Jazbec
authors.initials: PJ
authors.lastname: Križman
authors.forename: Aleksandra Domanjko
authors.initials: AD
authors.lastname: Petrič
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002318:Cardiovascular Diseases / Q000097:blood / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006333:Heart Failure / Q000097:blood; D008297:Male; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D000975:Antioxidants; D020097:Natriuretic Peptide, Brain; D014451:Ubiquinone; C024989:coenzyme Q10