title: Pax8, Napsin A, and CD10 as Immunohistochemical Markers of Canine Renal Cell Carcinoma.
abstract: Pax8, napsin A, and CD10 are useful immunohistochemical markers of human renal cell carcinoma (RCC); however, their diagnostic utility in canine RCC is unclear. Forty formalin-fixed paraffin-embedded renal cell carcinomas from dogs (15 papillary, 12 solid, and 13 tubular) and 10 metastases were evaluated for expression of Pax8, napsin A, and CD10. Thirty-nine (98%), 24 (60%), and 19 (50%) tumors expressed Pax8 (nuclear labeling), napsin A (cytoplasmic labeling), and CD10 (cytoplasmic and membranous labeling), respectively. Pax8 was expressed in 92% of solid, 100% of papillary, and 100% of tubular tumors. Napsin A was expressed in 58% of solid, 60% of papillary, and 62% of tubular RCC. CD10 was expressed in 33% of solid, 47% of papillary, and 62% of tubular RCC. Pax8 was expressed in 80% of the metastatic tumors, napsin A in 60%, and CD10 in 50%. Additionally, Pax8 immunoreactivity was stronger overall than that of napsin A or CD10. In summary, Pax8 is a more sensitive marker than napsin A or CD10 for primary and metastatic canine RCC; its nuclear and more intense reactivity also makes it easier to interpret. Tubular and papillary RCCs were more likely than solid RCC to express all 3 markers. These findings highlight the utility of Pax8 as an immunohistochemical marker in diagnosing all major subtypes of canine primary and metastatic renal cell carcinoma.
authors.affiliation: 1 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Peat
authors.affiliation: 2 NCI, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.|3 Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
authors.forename: Elijah F
authors.initials: EF
authors.lastname: Edmondson
authors.affiliation: 1 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Miller
authors.affiliation: 1 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.
authors.forename: Dee M
authors.initials: DM
authors.lastname: DuSold
authors.affiliation: 1 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.
authors.forename: José A
authors.initials: JA
authors.lastname: Ramos-Vara
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D004285:Dogs; D005260:Female; D007680:Kidney Neoplasms / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D015260:Neprilysin / Q000276:immunology / Q000378:metabolism*; D000072636:PAX8 Transcription Factor / Q000276:immunology / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D000072636:PAX8 Transcription Factor; D016282:Aspartic Acid Endopeptidases; D015260:Neprilysin
keywords: CD10; Pax8; dogs; immunohistochemistry; napsin A; neoplasia; renal cell carcinoma