title: The soybean GmSNAP18 gene underlies two types of resistance to soybean cyst nematode.
abstract: Two types of resistant soybean (Glycine max (L.) Merr.) sources are widely used against soybean cyst nematode (SCN, Heterodera glycines Ichinohe). These include Peking-type soybean, whose resistance requires both the rhg1-a and Rhg4 alleles, and PI 88788-type soybean, whose resistance requires only the rhg1-b allele. Multiple copy number of PI 88788-type GmSNAP18, GmAAT, and GmWI12 in one genomic segment simultaneously contribute to rhg1-b resistance. Using an integrated set of genetic and genomic approaches, we demonstrate that the rhg1-a Peking-type GmSNAP18 is sufficient for resistance to SCN in combination with Rhg4. The two SNAPs (soluble NSF attachment proteins) differ by only five amino acids. Our findings suggest that Peking-type GmSNAP18 is performing a different role in SCN resistance than PI 88788-type GmSNAP18. As such, this is an example of a pathogen resistance gene that has evolved to underlie two types of resistance, yet ensure the same function within a single plant species.
authors.affiliation: Department of Plant, Soil and Agricultural Systems, Southern Illinois University, 1205 Lincoln Drive RM176, Carbondale, Illinois 62901, USA.
authors.forename: Shiming
authors.initials: S
authors.lastname: Liu
authors.affiliation: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA.
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Kandoth
authors.affiliation: Department of Plant, Soil and Agricultural Systems, Southern Illinois University, 1205 Lincoln Drive RM176, Carbondale, Illinois 62901, USA.
authors.forename: Naoufal
authors.initials: N
authors.lastname: Lakhssassi
authors.affiliation: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Plant, Soil and Agricultural Systems, Southern Illinois University, 1205 Lincoln Drive RM176, Carbondale, Illinois 62901, USA.
authors.forename: Vincent
authors.initials: V
authors.lastname: Colantonio
authors.affiliation: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Heinz
authors.affiliation: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA.
authors.forename: Greg
authors.initials: G
authors.lastname: Yeckel
authors.affiliation: Department of Plant, Soil and Agricultural Systems, Southern Illinois University, 1205 Lincoln Drive RM176, Carbondale, Illinois 62901, USA.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Plant, Soil and Agricultural Systems, Southern Illinois University, 1205 Lincoln Drive RM176, Carbondale, Illinois 62901, USA.
authors.forename: Sadia
authors.initials: S
authors.lastname: Bekal
authors.affiliation: Generation Biotech, Lawrenceville, New Jersey 08648, USA.
authors.forename: Johannes
authors.initials: J
authors.lastname: Dapprich
authors.affiliation: GenXPro-GmbH, Altenhöferallee 3, 60438 Frankfurt am Main, Germany.
authors.forename: Bjorn
authors.initials: B
authors.lastname: Rotter
authors.affiliation: Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cianzio
authors.affiliation: Division of Plant Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA.
authors.forename: Melissa G
authors.initials: MG
authors.lastname: Mitchum
authors.affiliation: Department of Plant, Soil and Agricultural Systems, Southern Illinois University, 1205 Lincoln Drive RM176, Carbondale, Illinois 62901, USA.
authors.forename: Khalid
authors.initials: K
authors.lastname: Meksem
mesh_terms: D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D006239:Haplotypes; D059014:High-Throughput Nucleotide Sequencing; D006790:Host-Parasite Interactions; D054643:INDEL Mutation; D008957:Models, Genetic; D009348:Nematoda / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D030262:Soybean Proteins / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000469:parasitology*
chemical_list: D018744:DNA, Plant; D030262:Soybean Proteins