title: Biocompatibility of PCL/PLGA-BCP porous scaffold for bone tissue engineering applications.
abstract: In this study, biomimic porous polycaprolactone/poly (lactide-co-glycolide) loading biphasic tricalcium phosphate (PCL/PLGA-BCP) scaffolds were fabricated successfully by solvent evaporation method. The distribution of biphasic tricalcium phosphate (BCP) in polycaprolactone/poly (lactide-co-glycolide) (PCL/PLGA) scaffold was confirmed by micro-computed tomography (micro-CT) scanning, scanning electron microscope (SEM) observation and Energy-dispersive X-ray Spectroscopy (EDS) analysis. The hydrophilicity of the scaffolds was confirmed by contact angle measurement. In in vitro experiments, proliferation of human bone marrow mesenchymal stem cell (hBMSCs) and its osteoblastic differentiation on scaffold were assessed for 1, 2 and 3 weeks using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence observation, hematoxylin & eosin (H&E) staining and real-time polymerase chain reaction (RT-PCR). In in vivo experiments, ossification was observed using micro-CT analysis and histological staining.
authors.affiliation: a Tissue Engineering and Regenerative Medicine Laboratory, Biomedical Engineering Department , International University, Vietnam National University-Ho Chi Minh City (VNU-HCMC) , Ho Chi Minh City , Vietnam.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Thi Hiep
authors.affiliation: a Tissue Engineering and Regenerative Medicine Laboratory, Biomedical Engineering Department , International University, Vietnam National University-Ho Chi Minh City (VNU-HCMC) , Ho Chi Minh City , Vietnam.
authors.forename: Huynh
authors.initials: H
authors.lastname: Chan Khon
authors.affiliation: c Institute of Applied Materials Science , Vietnam Academy of Science and Technology , Ho Chi Minh City , Vietnam.|d Graduate University of Science and Technology , Vietnam Academy of Science and Technology , Hanoi , Vietnam.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Dai Hai
authors.affiliation: b Department of Regenerative Medicine, College of Medicine , Soonchunhyang University , Cheonan , South Korea.
authors.forename: Lee
authors.initials: L
authors.lastname: Byong-Taek
authors.affiliation: a Tissue Engineering and Regenerative Medicine Laboratory, Biomedical Engineering Department , International University, Vietnam National University-Ho Chi Minh City (VNU-HCMC) , Ho Chi Minh City , Vietnam.
authors.forename: Vo
authors.initials: V
authors.lastname: Van Toi
authors.affiliation: e Faculty of Odonto-Stomatology , University of Medicine and Pharmacy , Ho Chi Minh City , Vietnam.
authors.forename: Le
authors.initials: L
authors.lastname: Thanh Hung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014057:Tomography, X-Ray Computed
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D011091:Polyesters; C553659:polycaprolactone-co-lactide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C018392:tricalcium phosphate
keywords: Poly (lactide-co-glycolide); biphasic calcium phosphate; bone regeneration; polycaprolactone