title: Discrimination of Four Marine Biofilm-Forming Bacteria by LC-MS Metabolomics and Influence of Culture Parameters.
abstract: Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC-MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC-MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.
authors.affiliation: Université de Toulon , MAPIEM, EA 4323, La Garde Cedex 83130, France.
authors.forename: Laurie
authors.initials: L
authors.lastname: Favre
authors.affiliation: Université de Toulon , MAPIEM, EA 4323, La Garde Cedex 83130, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Ortalo-Magné
authors.affiliation: CNRS, Aix Marseille Univ , IRD, Avignon Univ. Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale, Station marine d'Endoume, Marseille 13007, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Greff
authors.affiliation: CNRS, Aix Marseille Univ , IRD, Avignon Univ. Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale, Station marine d'Endoume, Marseille 13007, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Pérez
authors.affiliation: CNRS, Aix Marseille Univ , IRD, Avignon Univ. Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale, Station marine d'Endoume, Marseille 13007, France.|National University of Ireland Galway , School of Chemistry, Marine Biodiscovery, Galway, Ireland.
authors.forename: Olivier P
authors.identifier: 0000-0002-5708-1409
authors.initials: OP
authors.lastname: Thomas
authors.affiliation: Aix-Marseille Univ , INSERM, INRA, NORT, Marseille 13005, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Martin
authors.affiliation: Université de Toulon , MAPIEM, EA 4323, La Garde Cedex 83130, France.
authors.forename: Gérald
authors.identifier: 0000-0001-5760-6394
authors.initials: G
authors.lastname: Culioli
mesh_terms: D001419:Bacteria / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D018441:Biofilms; D015415:Biomarkers / Q000032:analysis; D002853:Chromatography, Liquid; D008386:Marine Biology; D055432:Metabolomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers
keywords: MS/MS networking; biofilms; liquid chromatography−mass spectrometry; marine bacteria; metabolomics; ornithine lipids; polyamines