title: Characterization of two novel antimicrobial peptides from the cuticular extracts of the ant Trichomyrmex criniceps (Mayr), (Hymenoptera: Formicidae).
abstract: Antimicrobial peptides (AMPs) from cuticular extracts of worker ants of Trichomyrmex criniceps (Mayr, Hymenoptera: Formicidae) were isolated and evaluated for their antimicrobial activity. Eight peptides ranging in mass from 804.42 to 1541.04 Da were characterized using a combination of analytical and bioinformatics approach. All the eight peptides were novel with no similarity to any of the AMPs archived in the Antimicrobial Peptide Database. Two of the eight novel peptides, the smallest and the largest by mass were named Crinicepsin-1 and Crinicepsin-2 and were chemically synthesized by solid phase peptide synthesis. The two synthetic peptides had antibacterial and weak hemolytic activity.
authors.affiliation: Department of Applied Zoology, School of Biological Sciences, Kuvempu University, Shankaraghatta, Shivamogga, Karnataka, India.|Department of Agricultural Entomology and School of Ecology & Conservation, University of Agricultural Sciences, Bengaluru, Karnataka, India.
authors.forename: Nagachaitanya
authors.initials: N
authors.lastname: Bhagavathula
authors.affiliation: Department of Applied Zoology, School of Biological Sciences, Kuvempu University, Shankaraghatta, Shivamogga, Karnataka, India.
authors.forename: Venkateshwarlu
authors.initials: V
authors.lastname: Meedidoddi
authors.affiliation: Department of Biology and Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada.
authors.forename: Simon
authors.initials: S
authors.lastname: Bourque
authors.affiliation: Department of Chemical Biology, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India.
authors.forename: Reshmy
authors.initials: R
authors.lastname: Vimaladevi
authors.affiliation: Department of Chemical Biology, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India.
authors.forename: Santoshkumar
authors.initials: S
authors.lastname: Kesavakurup
authors.affiliation: Department of Biology and Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada.
authors.forename: Dayanandan
authors.initials: D
authors.lastname: Selvadurai
authors.affiliation: Veterinary Biotechnology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: Sameer
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: Department of Agricultural Entomology and School of Ecology & Conservation, University of Agricultural Sciences, Bengaluru, Karnataka, India.
authors.forename: Chandrashekara
authors.initials: C
authors.lastname: Krishnappa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D001000:Ants / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D019476:Insect Proteins / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D019476:Insect Proteins; D009842:Oligopeptides; D014020:Tissue Extracts; C000617795:crinicepsin-1; C000617796:crinicepsin-2
keywords: Trichomyrmex criniceps; antimicrobial activity; antimicrobial peptides; hemolytic activity; solid-phase peptide synthesis