title: Nucleic acid protocols: Extraction and optimization.
abstract: Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic sources exploiting the physical and chemical properties of nucleic acids. Furthermore, this protocol showed that DNA and RNA are under triple protection (i.e. EDTA, SDS and NaCl) during lysis step, and this environment is improper for RNase to have DNA liberated of RNA and even for DNase to degrade the DNA. Therefore, the complete removal of RNA under RNase influence is achieved when RNase is added after DNA extraction, which gives optimal quality with any protocols. Similarly, DNA contamination in an isolated RNA is degraded by DNase to obtain high-quality RNA. Our protocol is the protocol of choice in terms of simplicity, recovery time, environmental safety, amount, purity, PCR and RT-PCR applicability.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, Beijing 100193, China; Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
authors.forename: Saeed
authors.initials: S
authors.lastname: El-Ashram
authors.affiliation: College of Science and Arts in Unaizah, Qassim University, Unaizah, Saudi Arabia; College of Applied Health Sciences in Ar Rass, Qassim University, Ar Rass 51921, Saudi Arabia.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Al Nasr
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, Beijing 100193, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
keywords: DNA; DNase; Prokaryotic and eukaryotic sources; RNA; RNase