title: Computational Prediction of Intrinsic Disorder in Proteins.
abstract: Computational prediction of intrinsically disordered proteins (IDPs) is a mature research field. These methods predict disordered residues and regions in an input protein chain. More than 60 predictors of IDPs have been developed. This unit defines computational prediction of intrinsic disorder, summarizes major types of predictors of disorder, and provides details about three accurate and recently released methods. We demonstrate the use of these methods to predict intrinsic disorder for several illustrative proteins, provide insights into how predictions should be interpreted, and quantify and discuss predictive performance. Predictions can be freely and conveniently obtained using webservers. We point to the availability of databases that provide access to annotations of intrinsic disorder determined by structural studies and putative intrinsic disorder pre-computed by computational methods. Lastly, we also summarize experimental methods that can be used to validate computational predictions. © 2017 by John Wiley & Sons, Inc.
authors.affiliation: Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.
authors.forename: Fanchi
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.|Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Uversky
authors.affiliation: Department of Computer Science, Virginia Commonwealth University, Richmond, USA.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Kurgan
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D011487:Protein Conformation; D020539:Sequence Analysis, Protein
chemical_list: D064267:Intrinsically Disordered Proteins
keywords: intrinsic disorder; intrinsically disordered protein; prediction