title: Neurite outgrowth in cultured mouse pelvic ganglia - Effects of neurotrophins and bladder tissue.
abstract: Neurotrophic factors regulate survival and growth of neurons. The urinary bladder is innervated via both sympathetic and parasympathetic neurons located in the major pelvic ganglion. The aim of the present study was to characterize the effects of the neurotrophins nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of sympathetic and parasympathetic neurites from the female mouse ganglion. The pelvic ganglion was dissected out and attached to a petri dish and cultured in vitro. All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher stimulatory effect on parasympathetic ganglion cells. The neurotrophin receptors TrkA, TrkB and TrkC were all expressed in neurons of the ganglia. Co-culture of ganglia with urinary bladder tissue, but not diaphragm tissue, increased the sprouting rate of neurites. Active forms of BDNF and NT-3 were detected in urinary bladder tissue using western blotting whereas tissue from the diaphragm expressed NGF. Neurite outgrowth from the pelvic ganglion was inhibited by a TrkB receptor antagonist. We therefore suggest that the urinary bladder releases trophic factors, including BDNF and NT-3, which regulate neurite outgrowth via activation of neuronal Trk-receptors. These findings could influence future strategies for developing pharmaceuticals to improve re-innervation due to bladder pathologies.
authors.affiliation: Department of Experimental Medical Science, Lund University, Lund, Sweden; Department of Biology, Lund University, Lund, Sweden. Electronic address: mari.ekman@med.lu.se.
authors.forename: Mari
authors.initials: M
authors.lastname: Ekman
authors.affiliation: Department of Experimental Medical Science, Lund University, Lund, Sweden; Department of Urology, The Sixth Affiliated Hospital of Guangzhou Medical University, Guang Dong, China.
authors.forename: Baoyi
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Experimental Medical Science, Lund University, Lund, Sweden.
authors.forename: Karl
authors.initials: K
authors.lastname: Swärd
authors.affiliation: Department of Urology, Clinical Sciences, Lund University, Lund, Sweden.
authors.forename: Bengt
authors.initials: B
authors.lastname: Uvelius
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage / Q000378:metabolism*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003964:Diaphragm / Q000294:innervation; D005260:Female; D005725:Ganglia, Autonomic / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000378:metabolism*; D000071446:Neuronal Outgrowth / Q000187:drug effects / Q000502:physiology*; D020933:Neurotrophin 3 / Q000008:administration & dosage / Q000378:metabolism*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010388:Pelvis; D011467:Prostate / Q000294:innervation; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001743:Urinary Bladder / Q000294:innervation*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020933:Neurotrophin 3; D020932:Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases
keywords: BNDF; NGF; NT-3; Parasympathetic; Sympathetic; Trk receptors