title: Association of cytochrome P4502E1 and NAD(P)H:quinone oxidoreductase 1 genetic polymorphisms with susceptibility to large artery atherosclerotic ischemic stroke: a case-control study in the Turkish population.
abstract: Stroke, a major cause of death and disability, is described as interruption or severe reduction of blood flow in cerebral arteries. Oxidative stress plays an important role in the pathogenesis of atherosclerosis and carotid atherosclerosis is a risk factor for stroke. Combination of multiple environmental and genetic risk factors is thought to increase stroke. Therefore, investigation of the polymorphisms of enzymes is of crucial importance to determine the molecular etiology of the disease. To test this hypothesis, we performed a case-control study in which we compared the distribution of CYP2E1 and NQO1 genotypes between 245 large artery atherosclerotic ischemic stroke patients and 145 controls, using PCR-RFLP. A significant difference was observed between stroke patients and controls with respect to the CYP2E1*5B genotype (odds ratio; OR 8.069, P = 0.011) and allele (OR 7.876, P = 0.011) distribution. However, this polymorphism was not a significant predictor of disease status in logistic regression analysis. NQO1*2 polymorphism genotype distribution was significantly different between patients and controls (P = 0.027) and heterozygote *1*2 genotype was found to be a protective factor against large artery atherosclerotic ischemic stroke in logistic regression analysis (OR 0.562, P = 0.018). This is the first study conducted regarding the association of CYP2E1 and NQO1 genetic polymorphisms and large artery atherosclerotic ischemic stroke risk in Turkish population.
authors.affiliation: Department of Molecular Biology and Genetics, Joint Graduate Program in Biochemistry, Middle East Technical University, 06800, Ankara, Turkey.|Food Safety and Agricultural Research Center, Akdeniz University, Antalya, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Türkanoğlu Özçelik
authors.affiliation: Department of Biomedical Engineering, TOBB University of Economics and Technology, Ankara, Turkey.
authors.forename: Birsen
authors.initials: B
authors.lastname: Can Demirdöğen
authors.affiliation: Department of Neurology, Gülhane Medical Faculty, University of Health Sciences, Ankara, Turkey.
authors.forename: Şeref
authors.initials: Ş
authors.lastname: Demirkaya
authors.affiliation: Department of Molecular Biology and Genetics, Joint Graduate Program in Biochemistry, Middle East Technical University, 06800, Ankara, Turkey. bioorhan@metu.edu.tr.
authors.forename: Orhan
authors.initials: O
authors.lastname: Adalı
mesh_terms: D000368:Aged; D050197:Atherosclerosis / Q000235:genetics*; D002545:Brain Ischemia / Q000235:genetics*; D016022:Case-Control Studies; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D011110:Polymorphism, Genetic; D020521:Stroke / Q000235:genetics*; D014421:Turkey; D044465:White People / Q000235:genetics
chemical_list: D019392:Cytochrome P-450 CYP2E1; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human
keywords: CYP2E1; Large artery atherosclerotic ischemic stroke; NQO1; Polymorphism; Turkish population