title: Association of Glu298Asp Polymorphism of Endothelial NO Synthase Gene with Metabolic Syndrome Development: a Pilot Study.
abstract: We studied association of single nucleotide polymorphism Glu298Asp (rs1799983) of the NOS3 gene with the risk of metabolic syndrome in the Slavic population. Blood samples were obtained from 128 patients with metabolic syndrome and 100 healthy individuals. Polymorphism Glu298Asp of the NOS3 gene was genotyped by allele-specific PCR. Allele Asp (OR=1.95, 95%CI 1.29-2.95, p=0.007) and genotype Asp/Asp (OR=2.56, 95%CI 0.98-6.72, p=0.04) were associated with the risk of metabolic syndrome in Slavic population. Patients with metabolic syndrome carrying genotype Asp/Asp had higher serum endothelin-1 level in comparison with Glu/Asp and Glu/Glu carriers.
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia. nikola.fattahov@mail.ru.|Mental Health Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia. nikola.fattahov@mail.ru.
authors.forename: N S
authors.initials: NS
authors.lastname: Fattakhov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Skuratovskaya
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Vasilenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Kirienkova
authors.affiliation: Regional Hospital of the Kaliningrad Region, Kaliningrad, Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Zatolokin
authors.affiliation: Regional Hospital of the Kaliningrad Region, Kaliningrad, Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Mironyuk
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: L S
authors.initials: LS
authors.lastname: Litvinova
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019332:Endothelin-1 / Q000097:blood; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000201:enzymology / Q000235:genetics*; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide
chemical_list: D019332:Endothelin-1; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: endothelial NO synthase; endothelial dysfunction; metabolic syndrome; nitric oxide; single nucleotide polymorphism