title: Novel strategy for the facile enrichment of isopentenyl pyrophosphate in rat plasma via Ti4+-immobilized polydopamine@Fe3O4core-shell microspheres.
abstract: In this paper, a facile extraction strategy is reported for the analysis of isopentenyl pyrophosphate, a key isoprenoid, based on magnetic core-shell microspheres with Ti4+ion exterior walls coupled with liquid chromatography and tandem mass spectrometry. Because of their excellent hydrophilicity and biological compatibility, the polydopamine@Fe3O4-Ti4+microspheres display ideal isopentenyl pyrophosphate extraction efficiency. The technique includes three steps: sample loading, nonphosphate washing, and phosphate elution. Moreover, the microspheres can be regenerated by thorough washing with a specific solvent and can be reused multiple times. The liquid chromatography with tandem mass spectrometry separation was performed on a Welch Ultimate® XB-C18column with a total chromatographic analysis time of 5 min; the analytical recovery was 98.52%. The proposed method was used to determine the isopentenyl pyrophosphate concentration in rat plasma samples collected from the Shanghai Chest Hospital. The results indicate the prospective value of the as-made microspheres for the sensitive and selective enrichment of phosphate compounds in complicated matrices.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P.R. China.
authors.forename: Yingjia
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Ling
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P.R. China.
authors.forename: Xueni
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P.R. China.
authors.forename: Gengli
authors.initials: G
authors.lastname: Duan
mesh_terms: D000818:Animals; D045782:Hemiterpenes / Q000097:blood*; D007211:Indoles; D008863:Microspheres; D009943:Organophosphorus Compounds / Q000097:blood*; D011108:Polymers; D051381:Rats
chemical_list: D045782:Hemiterpenes; D007211:Indoles; D009943:Organophosphorus Compounds; D011108:Polymers; C568283:polydopamine; C004809:isopentenyl pyrophosphate
keywords: hybrid solid-phase extraction; isopentenyl pyrophosphate; magnetic microspheres; titanium