title: The 2'-O-methyladenosine nucleoside modification gene OsTRM13 positively regulates salt stress tolerance in rice.
abstract: Stress induces changes of modified nucleosides in tRNA, and these changes can influence codon-anticodon interaction and therefore the translation of target proteins. Certain nucleoside modification genes are associated with regulation of stress tolerance and immune response in plants. In this study, we found a dramatic increase of 2'-O-methyladenosine (Am) nucleoside in rice seedlings subjected to salt stress and abscisic acid (ABA) treatment. We identified LOC_Os03g61750 (OsTRM13) as a rice candidate methyltransferase for the Am modification. OsTRM13 transcript levels increased significantly upon salt stress and ABA treatment, and the OsTrm13 protein was found to be located primarily to the nucleus. More importantly, OsTRM13 overexpression plants displayed improved salt stress tolerance, and vice versa, OsTRM13 RNA interference (RNAi) plants showed reduced tolerance. Furthermore, OsTRM13 complemented a yeast trm13Δ mutant, deficient in Am synthesis, and the purified OsTrm13 protein catalysed Am nucleoside formation on tRNA-Gly-GCC in vitro. Our results show that OsTRM13, encoding a rice tRNA nucleoside methyltransferase, is an important regulator of salt stress tolerance in rice.
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Youmei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Dongqin
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Junbao
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Xukai
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.affiliation: College of Horticulture and Forestry Sciences, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.|School of Biosciences, University of Melbourne, Parkville 3010 VIC, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
authors.affiliation: College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.|Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D055049:Salt Tolerance; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D010940:Plant Proteins; C024341:2'-O-methyladenosine; D000040:Abscisic Acid; D000241:Adenosine
keywords: 2′-O-methyladenosine; ABA; modified nucleoside; rice; salt stress tolerance; tRNA.