title: A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation.
abstract: In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe3+, followed by the restoration of luminescence upon the addition of Na2S. The probe, hereinafter referred to as 1-Fe3+, exhibited a linear range of detection for Na2S from 0.01 to 1.5mM, with a detection limit of 2.9μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1-Fe3+for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Chung-Nga
authors.initials: CN
authors.lastname: Ko
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; School of Metallurgical and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou, China. Electronic address: liujbgood@hotmail.com.
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China. Electronic address: Simonlee@umac.mo.
authors.forename: Simon Ming-Yuen
authors.initials: SM
authors.lastname: Lee
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China. Electronic address: duncanleung@umac.mo.
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. Electronic address: edmondma@hkbu.edu.hk.
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D007495:Iridium / Q000737:chemistry*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D059629:Signal-To-Noise Ratio; D013440:Sulfides / Q000737:chemistry / Q000302:isolation & purification*; D015027:Zebrafish
chemical_list: D013440:Sulfides; D007495:Iridium
keywords: Endogenous sulfide production; Iridum(III) complex; Sulfide detection in living organism