title: Improved campesterol production in engineered Yarrowia lipolytica strains.
abstract: OBJECTIVE
  To engineer Yarrowia lipolytica for improving the heterologous production of campesterol (a key precursor to manufacture pharmaceutical steroids).
  
  
  RESULTS
  By screening 7-dehydrocholesterol reductase (DHCR7) from diverse species, DHCR7 from Danio rerio was the best candidate for campesterol synthesis. Overexpression of ACL (ATP: citrate lyase) or POX2 (peroxisome acyl-CoA oxidase 2) were key to improving campesterol production. The highest yield of campesterol was 942 mg/l was with the strain overexpressing POX2 in a 5 l bioreactor via high cell density fermentation process with a restricted supply of carbon sourc, sunflower seed oil.
  
  
  CONCLUSIONS
  A promising platform to synthesize downstream steroid drugs was established. Efficient approaches were provided to improve the production of desired molecules in Y. lipolytica with high oil utilization efficiency.
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Mingdong
authors.initials: M
authors.lastname: Yao
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China. wenhai.xiao@tju.edu.cn.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China. wenhai.xiao@tju.edu.cn.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 92 Weijin Road, Tianjin, 300072, China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Yingjin
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D019149:Bioreactors / Q000382:microbiology; D053898:Biosynthetic Pathways / Q000235:genetics*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000096:biosynthesis; D015870:Gene Expression; D060847:Metabolic Engineering; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D010840:Phytosterols / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D025062:Yarrowia / Q000235:genetics* / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D010840:Phytosterols; D011994:Recombinant Proteins; C021273:campesterol; D002784:Cholesterol; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019995:7-dehydrocholesterol reductase; D001275:ATP Citrate (pro-S)-Lyase
keywords: 7-Dehydrocholesterol reductase; Campesterol; Metabolic engineering; Oil utilization; Synthetic biology; Yarrowia lipolytica