title: The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation.
abstract: Cell culture and cryopreservation are necessary for clinical therapy and cells storage. The addition of 10% (v/v) foetal bovine serum (FBS) to basal culture media has been common practice and is one of the most widely used methods. FBS media added with 10% DMSO (dimethyl sulfoxide) have also been used for cryopreservation cells. Ideally, FBS should be avoided because of high cost and bio-safety. Silk sericin has been used as a serum substitute and an additive due to its good hydrophilicity and biological safety. This article summarizes a few details about the processing of sericin and its application as a serum substitute or an additive for cell culture and cryopreservation media. Sericin can be a potential novel serum substitute or an additive for cell culture and cryopreservation media.
authors.affiliation: Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou, 215123, People's Republic of China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Cao
authors.affiliation: Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou, 215123, People's Republic of China. sericult@suda.edu.cn.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D047030:Sericins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001802:Blood Substitutes; D047030:Sericins
keywords: Cell culture; Cryopreservation; Recovery; Sericin; Serum