title: Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco.
abstract: It is currently possible to transfer a biosynthetic pathway from a plant to another organism. This system has been exploited to transfer the metabolic richness of certain plant species to other plants or even to more simple metabolic organisms such as yeast or bacteria for the production of high added value plant compounds. Another application is to bioconvert substrates into scarcer or biologically more interesting compounds, such as piceatannol and pterostilbene. These two resveratrol-derived stilbenes, which have very promising pharmacological activities, are found in plants only in small amounts. By transferring the human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) gene to tobacco hairy roots and cell cultures, we developed a system able to bioconvert exogenous t-resveratrol into piceatannol in quantities near to mg L-1. Similarly, after heterologous expression of resveratrol O-methyltransferase from Vitis vinifera (VvROMT) in tobacco hairy roots, the exogenous t-resveratrol was bioconverted into pterostilbene. We also observed that both bioconversions can take place in tobacco wild type hairy roots (pRiA4, without any transgene), showing that unspecific tobacco P450 hydroxylases and methyltransferases can perform the bioconversion of t-resveratrol to give the target compounds, albeit at a lower rate than transgenic roots.
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Av. Joan XXIII sn, 08028 Barcelona, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Hidalgo
authors.affiliation: Plant Proteomics and Functional Genomics Group, Department of Agrochemistry and Biochemistry, Faculty of Science, University of Alicante, Alicante, Spain.
authors.forename: Ascensión
authors.initials: A
authors.lastname: Martínez-Márquez
authors.affiliation: Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Moyano
authors.affiliation: Plant Proteomics and Functional Genomics Group, Department of Agrochemistry and Biochemistry, Faculty of Science, University of Alicante, Alicante, Spain.
authors.forename: Roque
authors.initials: R
authors.lastname: Bru-Martínez
authors.affiliation: Department of Plant Physiology, Campus Miguel de Unamuno, University of Salamanca, E-37007 Salamanca, Spain.
authors.forename: Purificación
authors.initials: P
authors.lastname: Corchete
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Av. Joan XXIII sn, 08028 Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Palazon
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D018929:Cell Culture Techniques; D065633:Cytochrome P-450 CYP1B1 / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013267:Stilbenes; C107773:pterostilbene; C041525:3,3',4,5'-tetrahydroxystilbene; D006899:Mixed Function Oxygenases; D065633:Cytochrome P-450 CYP1B1; D008780:Methyltransferases; D000077185:Resveratrol