title: Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel.
abstract: Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins using a mix of three different immobilized kinase inhibitors (Purvalanol B, Bisindolylmaleimide X, and (R)-3-(4-((1-Phenylethyl)amino)thieno[2,3-d]pyrimidin-6-yl)benzoic acid (SB6-060-05)) on sepharose bead columns. Raw data is deposited in the PRIDE database [1], project number PXD005286. Data presented () shows changes relative to untreated control for each biological replicate for the three cell lines.
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Voin
authors.initials: V
authors.lastname: Petrovic
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Camilla
authors.initials: C
authors.lastname: Olaisen
authors.affiliation: The Proteomics and Metabolomics Core Facility (PROMEC) at NTNU, Faculty of Medicine, Trondheim, Norway.
authors.forename: Animesh
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Anala
authors.initials: A
authors.lastname: Nepal
authors.affiliation: Department of Chemistry, Faculty of Natural Sciences and Technology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Steffen
authors.initials: S
authors.lastname: Bugge
authors.affiliation: Department of Chemistry and Material Technology, Faculty of Natural Sciences and Technology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Eirik
authors.initials: E
authors.lastname: Sundby
authors.affiliation: Department of Chemistry, Faculty of Natural Sciences and Technology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Bård Helge
authors.initials: BH
authors.lastname: Hoff
authors.affiliation: The Proteomics and Metabolomics Core Facility (PROMEC) at NTNU, Faculty of Medicine, Trondheim, Norway.
authors.forename: Geir
authors.initials: G
authors.lastname: Slupphaug
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Marit
authors.initials: M
authors.lastname: Otterlei