title: Oxidative modification and electrochemical inactivation of Escherichia coli upon cold atmospheric pressure plasma exposure.
abstract: Cold atmospheric pressure plasmas (CAPPs) are known to have bactericidal effects but the mechanism of their interaction with microorganisms remains poorly understood. In this study the bacteria Escherichia coli were used as a model and were exposed to CAPPs. Different gas compositions, helium with or without adjunctions of nitrogen or oxygen, were used. Our results indicated that CAPP induced bacterial death at decontamination levels depend on the duration, post-treatment storage and the gas mixture composition used for the treatment. The plasma containing O2 in the feeding gas was the most aggressive and showed faster bactericidal effects. Structural modifications of treated bacteria were observed, especially significant was membrane leakage and morphological changes. Oxidative stress caused by plasma treatment led to significant damage of E. coli. Biochemical analyses of bacterial macromolecules indicated massive intracellular protein oxidation. However, reactive oxygen and nitrogen species (RONS) are not the only actors involved in E. coli's death, electrical field and charged particles could play a significant role especially for He-O2 CAPP.
authors.affiliation: UMR 5254, IPREM, Université de Pau et des pays de l'Adour, Pau, France.
authors.forename: Marlène
authors.initials: M
authors.lastname: Dezest
authors.affiliation: UMR 5254, IPREM, Université de Pau et des pays de l'Adour, Pau, France.
authors.forename: Anne-Laure
authors.identifier: http://orcid.org/0000-0002-3315-5100
authors.initials: AL
authors.lastname: Bulteau
authors.affiliation: NSysA group, ENSCBP, CNRS UMR 5255, ISM, Université de Bordeaux, Pessac, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Quinton
authors.affiliation: UMR 5254, IPREM, Université de Pau et des pays de l'Adour, Pau, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Chavatte
authors.affiliation: UMR 5254, IPREM, Université de Pau et des pays de l'Adour, Pau, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Le Bechec
authors.affiliation: INSERM UMR-1048 I2MC, Université Paul Sabatier, Toulouse, France.
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Cambus
authors.affiliation: NSysA group, ENSCBP, CNRS UMR 5255, ISM, Université de Bordeaux, Pessac, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Arbault
authors.affiliation: INSERM UMR-1048 I2MC, Université Paul Sabatier, Toulouse, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Nègre-Salvayre
authors.affiliation: UMR 5254, IPREM, Université de Pau et des pays de l'Adour, Pau, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Clément
authors.affiliation: Faculté de Chirurgie Dentaire de Toulouse, centre Hospitalier Universitaire de Toulouse, Université Paul Sabatier, Toulouse, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Cousty
mesh_terms: D001274:Atmospheric Pressure; D003666:Decontamination; D004926:Escherichia coli / Q000187:drug effects*; D006371:Helium / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D009584:Nitrogen / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D058626:Plasma Gases / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D058626:Plasma Gases; D017382:Reactive Oxygen Species; D006371:Helium; D009584:Nitrogen