title: SMI-Ribosome inactivating protein conjugates selectively inhibit tumor cell growth.
abstract: Cell-targeting conjugates of Saporin 6, a ribosome inactivating protein (RIP), were prepared using the Saporin Ala 157 Cys mutant, a small molecule inhibitor (SMI) of integrins αvβ3/αvβ5, and a potent cytotoxin, auristatin F (AF). The conjugates selectively and potently inhibited proliferation of tumor cells expressing the target integrins. We anticipate that the small molecule-RIP bioconjugate approach can be broadly applied using other small molecule drugs.
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. ssinha@rockefeller.edu.
authors.forename: Saumya
authors.initials: S
authors.lastname: Roy
authors.forename: Jun Y
authors.initials: JY
authors.lastname: Axup
authors.forename: Jane S
authors.initials: JS
authors.lastname: Forsyth
authors.forename: Rajib K
authors.initials: RK
authors.lastname: Goswami
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Hutchins
authors.forename: Krishna M
authors.initials: KM
authors.lastname: Bajuri
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Kazane
authors.forename: Vaughn V
authors.initials: VV
authors.lastname: Smider
authors.forename: Brunhilde H
authors.initials: BH
authors.lastname: Felding
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Sinha