title: Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol?
abstract: The aim of the present study was to determine whether the reduction in liver fat previously observed in our laboratory in a cohort of rats which had been fed an obesogenic diet was mediated by changes in the expression of microRNA (miRNA)-103-3p, miRNA-107-3p and miRNA-122-5p, which represent 70% of total miRNAs in the liver, as well as in their target genes. The expression of the three analysed miRNAs was reduced in rats treated with resveratrol. A reduction in sterol-regulatory element binding protein 1 (SREBP1) and an increase in carnitine palmitoyltransferase 1a (CPT1a) were observed in resveratrol-treated rats. No changes were found in fatty acid synthase (FAS). In cultured hepatocytes, SREBP1 protein was increased after the transfection of each miRNA. FAS protein expression was decreased after the transfection of miRNA-122-5p, and CPT1a protein was down-regulated by the over-expression of miRNA-107-3p. This study provides new evidences which show that srebf1 is a target gene for miRNA-103-3p and miRNA-107-3p, fasn a target gene for miRNA-122-5p and cpt1a a target gene for miRNA-107-3p. Moreover, the reduction in liver steatosis induced by resveratrol in rats fed an obesegenic diet is mediated, at least in part, by the increase in CPT1a protein expression and activity, via a decrease in miRNA-107-3p expression.
authors.affiliation: 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain; anajadraque@gmail.com (A.G.); jonatan.miranda@ehu.eus (J.M.); itziareseberri@hotmail.com (I.E.); mariapuy.portillo@ehu.eus (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina; maidagon@fbcb.unl.edu.ar. anajadraque@gmail.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Gracia
authors.affiliation: 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain; anajadraque@gmail.com (A.G.); jonatan.miranda@ehu.eus (J.M.); itziareseberri@hotmail.com (I.E.); mariapuy.portillo@ehu.eus (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina; maidagon@fbcb.unl.edu.ar. alfredo.fernandez@ehu.eus.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fernández-Quintela
authors.affiliation: 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain; anajadraque@gmail.com (A.G.); jonatan.miranda@ehu.eus (J.M.); itziareseberri@hotmail.com (I.E.); mariapuy.portillo@ehu.eus (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina; maidagon@fbcb.unl.edu.ar. jonatan.miranda@ehu.eus.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Miranda
authors.affiliation: 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain; anajadraque@gmail.com (A.G.); jonatan.miranda@ehu.eus (J.M.); itziareseberri@hotmail.com (I.E.); mariapuy.portillo@ehu.eus (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina; maidagon@fbcb.unl.edu.ar. itziareseberri@hotmail.com.
authors.forename: Itziar
authors.initials: I
authors.lastname: Eseberri
authors.affiliation: 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain; anajadraque@gmail.com (A.G.); jonatan.miranda@ehu.eus (J.M.); itziareseberri@hotmail.com (I.E.); mariapuy.portillo@ehu.eus (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina; maidagon@fbcb.unl.edu.ar. maidagon@fbcb.unl.edu.ar.
authors.forename: Marcela
authors.initials: M
authors.lastname: González
authors.affiliation: 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain; anajadraque@gmail.com (A.G.); jonatan.miranda@ehu.eus (J.M.); itziareseberri@hotmail.com (I.E.); mariapuy.portillo@ehu.eus (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina; maidagon@fbcb.unl.edu.ar. mariapuy.portillo@ehu.eus.
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D059305:Diet, High-Fat; D015536:Down-Regulation; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005234:Fatty Liver / Q000139:chemically induced / Q000188:drug therapy / Q000235:genetics*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C000602463:MIRN103 microRNA, rat; C579315:MIRN107 microRNA, rat; C580643:MIRN122 microRNA, rat; D035683:MicroRNAs; C497389:Srebf1 protein, rat; D051780:Sterol Regulatory Element Binding Protein 1; D013267:Stilbenes; D002334:Carnitine O-Palmitoyltransferase; C583748:carnitine palmitoyltransferase-1a, rat; D064429:Fatty Acid Synthases; D000077185:Resveratrol
keywords: liver; miRNA-103; miRNA-107; miRNA-122; rat; resveratrol; steatosis