title: One-pot preparation of mercaptotetrazole-silica hybrid monoliths by the thiol-ene click reaction for mixed-mode capillary liquid chromatography.
abstract: A novel mercaptotetrazole-silica hybrid monolithic column was prepared for capillary liquid chromatography, in which the thiol-end mercaptotetrazole was mixed with hydrolyzed γ-methacryloxypropyltrimethoxysilane and tetramethyloxysilane for the co-polycondensation and thiol-ene click reaction in a one-pot process. The effects of the molar ratio of silanes, the amount of mercaptotetrazole, and the volume of porogen on the morphology, permeability and pore properties of the as-prepared mercaptotetrazole-silica hybrid monoliths were investigated in detail. A series of test compounds including alkylbenzenes, amides and anilines were employed for evaluating the retention behaviors of the mercaptotetrazole-silica hybrid monolithic columns. The results demonstrated that the mercaptotetrazole-silica hybrid monoliths exhibited hydrophobic, hydrophilic as well as ion-exchange interaction. The run-to-run, column-to-column and batch-to-batch reproducibilities of the mercaptotetrazole-silica hybrid monoliths were satisfactory with the relative standard deviations less than 1.4 (n = 5), 3.9 (n = 3) and 4.0% (n = 5), respectively. In addition, the mercaptotetrazole-silica hybrid monolith was further applied to the separation of sulfonamides, nucleobases and protein tryptic digests. These successful applications confirmed the promising potential of the mercaptotetrazole-silica hybrid monolith in the separation of complex samples.
authors.affiliation: School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi, China.
authors.forename: Haojiang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Hu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Bian
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
authors.forename: Zian
authors.identifier: http://orcid.org/0000-0002-0866-0711
authors.initials: Z
authors.lastname: Lin
keywords: capillary liquid chromatography; hybrid monolithic column; mixed-mode; one-pot; thiol-ene click reaction