title: Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications.
abstract: Herein, novel hybrid nanomaterials were developed for wound dressing applications with antimicrobial properties. Electrospinning was used to fabricate a double layer nanocomposite nanofibrous mat consisting of an upper layer of poly(vinyl alcohol) and chitosan loaded with silver nanoparticles (AgNPs) and a lower layer of polyethylene oxide (PEO) or polyvinylpyrrolidone (PVP) nanofibers loaded with chlorhexidine (as an antiseptic). The top layer containing AgNPs, whose purpose was to protect the wound site against environmental germ invasion, was prepared by reducing silver nitrate to its nanoparticulate form through interaction with chitosan. The lower layer, which would be in direct contact with the injured site, contained the antibiotic drug needed to avoid wound infections which would otherwise interfere with the healing process. Initially, the upper layer was electrospun, followed sequentially by electrospinning the second layer, creating a bilayer nanofibrous mat. The morphology of the nanofibrous mats was studied by scanning electron microscopy and transmission electron microscopy, showing successful nanofiber production. X-ray diffraction confirmed the reduction of silver nitrate to AgNPs. Fourier transform infrared spectroscopy showed a successful incorporation of the material used in the produced nanofibrous mats. Thermal studies carried out by thermogravimetric analysis indicated that the PVP-drug-loaded layer had the highest thermal stability in comparison to other fabricated nanofibrous mats. Antimicrobial activities of the as-synthesized nanofibrous mats againstStaphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa, andCandida albicanswere determined using disk diffusion method. The results indicated that the PEO-drug-loaded mat had the highest antibacterial activity, warranting further attention for numerous wound-healing applications.
authors.affiliation: Materials Science and Technology Program, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors.forename: Alaa J
authors.initials: AJ
authors.lastname: Hassiba
authors.affiliation: School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: El Zowalaty
authors.affiliation: Department of Chemical Engineering; Department of Bioengineering, Northeastern University, Boston, MA, USA; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.affiliation: Center for Advanced Materials.
authors.forename: Aboubakr M
authors.initials: AM
authors.lastname: Abdullah
authors.affiliation: Department of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar University, Doha, Qatar.
authors.forename: Gheyath K
authors.initials: GK
authors.lastname: Nasrallah
authors.affiliation: Department of Mechanical Engineering, College of Engineering, University of Sharjah, Sharjah, United Arab Emirates.
authors.forename: Khalil Abdelrazek
authors.initials: KA
authors.lastname: Khalil
authors.affiliation: Center for Advanced Materials.
authors.forename: Adriaan S
authors.initials: AS
authors.lastname: Luyt
authors.affiliation: Materials Science and Technology Program, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Elzatahry
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000494:pharmacology / Q000627:therapeutic use*; D001458:Bandages; D002175:Candida / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012835:Silver Nitrate / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013818:Thermogravimetry; D014945:Wound Healing / Q000187:drug effects; D014946:Wound Infection / Q000188:drug therapy*; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D048271:Chitosan; D012835:Silver Nitrate
keywords: activity; antimicrobial; biomedical; electrospinning; nanofibers; nanomaterials; wound dressing