title: Expression Profiling and Identification of Novel SNPs in CatSper2 Gene and Their Influence on Sperm Motility Parameters in Bovines.
abstract: 122 randomly selected Vrindavani cattle were studied to detect polymorphism in four fragments of the CatSper2 gene that were comprised of exon 2, 4, 5, and 6 with flanking regions. Using PCR-SSCP and sequencing analysis, three SNPs (T157C, C273A, and A274C) in the first fragment, one SNP (C30G) in the second fragment, and two SNPs (T86G and T292C) in the fourth fragment were identified. The third fragment did not reveal any polymorphism. The SNPs were used for construction of haplotypes and three haplotypes were found. The least square analysis of variance revealed a significant (P < 0.01) effect of haplotype on all three motility parameters. The haplotype II and III were nonsignificantly different from each other while being significantly (P < 0.01) different from haplotype I. The nonsignificant difference of haplotype II with III can lead to a hypothesis that T>G or C>T SNPs may not play a role in sperm motility. However, when the comparison was made between haplotype I and II, it can be inferred that C>T SNP may have a role in sperm motility, as haplotype II has better motility parameters. Expression profiling of Catper2 gene revealed nonsignificant down regulation of CatSper2 gene in poor motility sperm compared to good motility sperm.
authors.affiliation: a Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: A
authors.initials: A
authors.lastname: Sivakumar
authors.affiliation: a Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: b Department of Animal Genetics and Breeding, Veterinary College , Bidar , India.
authors.forename: H M
authors.initials: HM
authors.lastname: Yathish
authors.affiliation: c Department of Animal Breeding and Genetics , OUAT , Bhubaneswar , India.
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Mishra
authors.affiliation: a Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: Rajendra Prasad
authors.initials: RP
authors.lastname: Modi
authors.affiliation: a Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: Rajni
authors.initials: R
authors.lastname: Chaudhary
authors.affiliation: a Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: Subuhi
authors.initials: S
authors.lastname: Khan
authors.affiliation: d Nutrition, Genetics and Biotechnology Division , Central Institute of Brackishwater Aquaculture , Chennai , India.
authors.forename: B
authors.initials: B
authors.lastname: Sivamani
authors.affiliation: e Division of Animal Reproduction, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: S K
authors.initials: SK
authors.lastname: Ghosh
authors.affiliation: f Division of Physiology & Climatology, Indian Veterinary Research Institute , Izatnagar , India.
authors.forename: Mihir
authors.initials: M
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism*; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods; D006239:Haplotypes / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D029589:Seminal Plasma Proteins / Q000235:genetics* / Q000378:metabolism*; D013081:Sperm Motility / Q000235:genetics*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D029589:Seminal Plasma Proteins
keywords: CatSper2; SNPs; cattle; expression PCR-SSCP