title: On-site removal of antibiotics and antibiotic resistance genes from leachate by aged refuse bioreactor: Effects of microbial community and operational parameters.
abstract: The abuse of antibiotics has raised the prevalence of antibiotic resistance, which will pose potential risk to human health. Leachate, generated during the landfill treatment of municipal solid waste, is the important hotspot of the antibiotics and antibiotic resistance genes (ARGs), and no effective on-site treatment has been put forward for preventing ARGs dissemination. Herein, the aged refuse bioreactor was employed to remove antibiotics and ARGs from leachate, and the great removal performance was observed. For the detected antibiotics, the total removal efficiency was about 76.75%, and sulfanilamide and macrolide were removed with high efficiencies (>80%). Among the target ARGs, tetracycline and macrolide resistance genes (tetM, tetQ and ermB) were eliminated with 1.2-2.0 orders of magnitude. The occurrences of ARGs did not correlated with water quality parameters such as COD, total nitrogen, ammonia, nitrate and nitrite, but closely linked to the variations of the bacterial community structure. Redundancy analysis (RDA) indicated the significant correlations between four genera and the distribution of ARGs, which implied that these key genera (including potential pathogens) drove the ARGs removal. Furthermore, the hydraulic loading test confirmed that the aged refuse bioreactor was capable of achieving high removal efficiencies even under shock loading for the higher loading was negative for the proliferations of potential ARGs hosts. This study suggested that aged refuse bioreactor could be a promising way for antibiotics and ARGs on-site removal from leachate.
authors.affiliation: Key Laboratory for Urban and Ecological Restoration of Shanghai, School of Ecology and Environmental Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Key Laboratory for Urban and Ecological Restoration of Shanghai, School of Ecology and Environmental Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory for Urban and Ecological Restoration of Shanghai, School of Ecology and Environmental Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Zhiting
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory for Urban and Ecological Restoration of Shanghai, School of Ecology and Environmental Sciences, East China Normal University, Shanghai 200241, China. Electronic address: bxie@des.ecnu.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Xie
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D019149:Bioreactors / Q000382:microbiology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004784:Environmental Monitoring; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005735:Garbage; D006801:Humans; D062611:Solid Waste; D064091:Waste Disposal Facilities / Q000592:standards*
chemical_list: D000900:Anti-Bacterial Agents; D062611:Solid Waste
keywords: Aged refused bioreactor; Antibiotic resistance genes; Antibiotics; Bacterial community; Correlations; Leachate on-site treatment