title: Overexpression of G6PD Represents a Potential Prognostic Factor in Clear Cell Renal Cell Carcinoma.
abstract: Glucose-6-phosphate dehydrogenase (G6PD) participates in glucose metabolism and it acts as the rate-limiting enzyme of the pentose phosphate pathway (PPP). Recently, G6PD dysregulation has been found in a variety of human cancers. Through analyzing published data in The Cancer Genome Atlas (TCGA), our pilot study indicated thatG6PDmRNA expression was significantly higher in advanced Fuhrman grade in clear cell renal cell carcinoma (ccRCC). These clues promoted us to further evaluate the expression profile of G6PD and its prognostic impact in patients with ccRCC. In this study, G6PD expression levels were analyzed in 149 human ccRCC and normal tissues using immunohistochemistry. The results showed that compared with that in the normal renal samples, G6PD was found highly expressed in 51.0% of ccRCC (p<0.05). High expression of G6PD was significantly correlated to tumor extent, lymph node metastasis, Fuhrman grade, and TNM stage of ccRCC (allp<0.05). Moreover, positive G6PD expression was associated with poorer overall survival in ccRCC (p<0.001). In Cox regression analyses, high expression of G6PD also could be an independent prognostic factor for overall survival in ccRCC (p=0.007). This study suggests that overexpression of G6PD is associated with advanced disease status and therefore may become an important prognosticator for poor outcomes in ccRCC, as well as a potential therapeutic target for developing effective treatment modalities.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Kunming Medical University, Yunnan, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of pathology, The Second Affiliated Hospital of Kunming Medical University, Yunnan, China.
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of pathology, The First Affiliated Hospital of Kunming Medical University, Yunnan, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Kunming Medical University, Yunnan, China.
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Han
authors.affiliation: Department of organ transplantation, The First Affiliated Hospital of Kunming Medical University, Yunnan, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Di
authors.affiliation: Department of organ transplantation, The First Affiliated Hospital of Kunming Medical University, Yunnan, China.
authors.forename: Fufei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Kunming Medical University, Yunnan, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Kunming Medical University, Yunnan, China.
authors.forename: Zihan
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Department of organ transplantation, The First Affiliated Hospital of Kunming Medical University, Yunnan, China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Kuang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Kunming Medical University, Yunnan, China.
authors.forename: Yuechun
authors.initials: Y
authors.lastname: Zhu
keywords: TCGA data mining; glucose-6-phosphate dehydrogenase; immunohistochemistry; prognosis biomarker; renal cell carcinoma