title: Development of Near-Isogenic Lines in a Parthenogenetically Reproduced Thrips Species,Frankliniella occidentalis.
abstract: Although near-isogenic lines (NILs) can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant NILs in the western flower thrips,Frankliniella occidentalis(Pergande) (Thysanoptera: Thripidae), with a haplo-diploid reproduction system. The resultantF. occidentalisNIL-R strain maintained a resistance ratio over 30,000-fold, which was comparable to its parental resistant strain, Spin-R. More importantly,F. occidentalisNIL-R shared 98.90% genetic similarity with its susceptible parental strain Ivf03. By developing this toolset, we are able to segregate individual resistance and facilitate the mechanistic study of insecticide resistances in phloem-feeding arthropods, a group of devastating pest species reproducing sexually as well as asexually.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Guangdi
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yanran
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: He
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Baoyun
authors.initials: B
authors.lastname: Xu
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Entomology, University of Kentucky Lexington, KY, USA.
authors.forename: Xuguo
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Wu
keywords: Frankliniella occidentalis; ISSR; insecticide resistance; near-isogenic lines; spinosad