title: Therapeutic effect of curcumin on experimental colitis mediated by inhibiting CD8+CD11c+cells.
abstract: OBJECTIVE
  To verify whether curcumin (Cur) can treat inflammatory bowel disease by regulating CD8+CD11c+cells.
  
  
  METHODS
  We evaluated the suppressive effect of Cur on CD8+CD11c+cells in spleen and Peyer's patches (PPs) in colitis induced by trinitrobenzene sulfonic acid. Mice with colitis were treated by 200 mg/kg Cur for 7 d. On day 8, the therapeutic effect of Cur was evaluated by visual assessment and histological examination, while co-stimulatory molecules of CD8+CD11c+cells in the spleen and PPs were measured by flow cytometry. The levels of interleukin (IL)-10, interferon (IFN)-γ and transforming growth factor (TGF)-β1 in spleen and colonic mucosa were determined by ELISA.
  
  
  RESULTS
  The disease activity index, colon weight, weight index of colon and histological score of experimental colitis were obviously decreased after Cur treatment, while the body weight and colon length recovered. After treatment with Cur, CD8+CD11c+cells were decreased in the spleen and PPs, and the expression of major histocompatibility complex II, CD205, CD40, CD40L and intercellular adhesion molecule-1 was inhibited. IL-10, IFN-γ and TGF-β1 levels were increased compared with those in mice with untreated colitis.
  
  
  CONCLUSIONS
  Cur can effectively treat experimental colitis, which is realized by inhibiting CD8+CD11c+cells.
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Zhao
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Huang
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Shao-Min
authors.initials: SM
authors.lastname: Cheng
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Min-Fang
authors.initials: MF
authors.lastname: Huang
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Yue
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Han-Lin
authors.initials: HL
authors.lastname: Xu
authors.affiliation: Hai-Mei Zhao, Shao-Min Cheng, School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China.
authors.forename: Duan-Yong
authors.initials: DY
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D039521:CD11c Antigen / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D003106:Colon; D003474:Curcumin / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D039521:CD11c Antigen; C000611649:IFNG protein, mouse; C556062:IL10 protein, mouse; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D007371:Interferon-gamma; D014302:Trinitrobenzenesulfonic Acid; D003474:Curcumin
keywords: CD11c; CD8; Curcumin; Experimental colitis; Therapeutic effect