title: Evaluation of the solvent water effect on high solids saccharification of alkali-pretreated sugarcane bagasse.
abstract: Solvent water is an essential factor for high solids enzymatic hydrolysis. To investigate its effect on substrate conversion efficiency in high solids hydrolysis of sugarcane bagasse (SCB), oleyl alcohol was used to partially substitute the solvent water. The results in batch hydrolysis tests in which diverse ratio of solvent water was replaced found that the majority of the substrate was insoluble. Then high solids fed-batch hydrolysis with the reaction solution mixed with solvent water and oleyl alcohol in the ratio of 3:1 (solids concentration correspond to 24% (w/v)) was carried out at the final real solids loading of 18% (w/v). The produced sugars were found to be less than pure water system, which indicated that water played a significant role in high solids hydrolysis process, and solids effect was related to the solvent water content.
authors.affiliation: College of Mechanical and Electrical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Mechanical and Electrical Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Minchao
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: yuanzh@ms.giec.ac.cn.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000468:Alkalies; D002482:Cellulose / Q000737:chemistry; D006868:Hydrolysis; D031786:Saccharum / Q000737:chemistry*; D012997:Solvents; D014867:Water
chemical_list: D000468:Alkalies; D012997:Solvents; D014867:Water; D002482:Cellulose
keywords: Alkali-pretreated SCB; Enzymatic saccharification; Fed-batch strategy; High solids loading; Oleyl alcohol; Solvent water