title: Fyn regulates multipolar-bipolar transition and neurite morphogenesis of migrating neurons in the developing neocortex.
abstract: Fyn is a non-receptor protein tyrosine kinase that belongs to Src family kinases. Fyn plays a critical role in neuronal migration, but the mechanism remains unclear. Here, we reported that suppression of Fyn expression in mouse cerebral cortex led to migration defects of both early-born and late-born neurons. Morphological analysis showed that loss of Fyn function impaired multipolar-bipolar transition of newly generated neurons and neurite formation in the early phase of migration. Moreover, Fyn inhibition increased the length of leading process and decreased the branching number of the migrating cortical neurons. Together, these results indicate that Fyn controls neuronal migration by regulating the cytoskeletal dynamics and multipolar-bipolar transition of newly generated neurons during cortical development.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yingxue
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: An
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yanle
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xinran
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xuzhao
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Rihua
authors.initials: R
authors.lastname: Cong
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China. Electronic address: csl_1359@126.com.
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, PR China. Electronic address: shantingzhao@hotmail.com.
authors.forename: Shanting
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement / Q000502:physiology*; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D019579:Neocortex / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016501:Neurites / Q000378:metabolism*; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D050809:POU Domain Factors / Q000378:metabolism; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D014162:Transfection
chemical_list: D009419:Nerve Tissue Proteins; D050809:POU Domain Factors; C074066:Pou3f2 protein, mouse; D049452:Green Fluorescent Proteins; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn
keywords: Fyn; RNAi; in utero electroporation; neuronal migration