title: Structural abnormalities in the primary somatosensory cortex and a normal behavioral profile in Contactin-5 deficient mice.
abstract: Contactin-5 (Cntn5) is an immunoglobulin cell adhesion molecule that is exclusively expressed in the central nervous system. In view of its association with neurodevelopmental disorders, particularly autism spectrum disorder (ASD), this study focused on Cntn5-positive areas in the forebrain and aimed to explore the morphological and behavioral phenotypes of the Cntn5 null mutant (Cntn5-/-) mouse in relation to these areas and ASD symptomatology. A newly generated antibody enabled us to elaborately describe the spatial expression pattern of Cntn5 in P7 wild type (Cntn5+/+) mice. The Cntn5 expression pattern included strong expression in the cerebral cortex, hippocampus and mammillary bodies in addition to described previously brain nuclei of the auditory pathway and the dorsal thalamus. Thinning of the primary somatosensory (S1) cortex was found in Cntn5-/-mice and ascribed to a misplacement of Cntn5-ablated cells. This phenotype was accompanied by a reduction in the barrel/septa ratio of the S1 barrel field. The structure and morphology of the hippocampus was intact in Cntn5-/-mice. A set of behavioral experiments including social, exploratory and repetitive behaviors showed that these were unaffected in Cntn5-/-mice. Taken together, these data demonstrate a selective role of Cntn5 in development of the cerebral cortex without overt behavioral phenotypes.
authors.affiliation: a Department of Translational Neuroscience , Brain Centre Rudolf Magnus, University Medical Centre Utrecht , Utrecht , the Netherlands.
authors.forename: Kristel T E
authors.initials: KTE
authors.lastname: Kleijer
authors.affiliation: a Department of Translational Neuroscience , Brain Centre Rudolf Magnus, University Medical Centre Utrecht , Utrecht , the Netherlands.
authors.forename: Denise
authors.initials: D
authors.lastname: van Nieuwenhuize
authors.affiliation: a Department of Translational Neuroscience , Brain Centre Rudolf Magnus, University Medical Centre Utrecht , Utrecht , the Netherlands.
authors.forename: Henk A
authors.initials: HA
authors.lastname: Spierenburg
authors.affiliation: a Department of Translational Neuroscience , Brain Centre Rudolf Magnus, University Medical Centre Utrecht , Utrecht , the Netherlands.
authors.forename: Sara
authors.initials: S
authors.lastname: Gregorio-Jordan
authors.affiliation: a Department of Translational Neuroscience , Brain Centre Rudolf Magnus, University Medical Centre Utrecht , Utrecht , the Netherlands.
authors.forename: Martien J H
authors.initials: MJH
authors.lastname: Kas
authors.affiliation: a Department of Translational Neuroscience , Brain Centre Rudolf Magnus, University Medical Centre Utrecht , Utrecht , the Netherlands.
authors.forename: J Peter H
authors.initials: JPH
authors.lastname: Burbach
mesh_terms: D000818:Animals; D000067877:Autism Spectrum Disorder / Q000235:genetics / Q000473:pathology; D001522:Behavior, Animal / Q000502:physiology*; D002540:Cerebral Cortex / Q000254:growth & development*; D058966:Contactins / Q000172:deficiency* / Q000378:metabolism; D006624:Hippocampus / Q000473:pathology; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000378:metabolism; D013003:Somatosensory Cortex / Q000002:abnormalities*
chemical_list: C000629348:Cntn5 protein, mouse; D058966:Contactins; D009419:Nerve Tissue Proteins
keywords: autism spectrum disorder; behavior; contactin-5; hippocampus; primary somatosensory cortex