title: A selective HPLC-MS/MS method for quantification of SND-117 in rat plasma and its application to a pharmacokinetic study.
abstract: Rheumatoid arthritis (RA), a chronic systemic inflammatory disorder affects many adults. Sinomenine, a natural product, has been clinically available for the treatment of RA in China. SND-117, a sinomenine derivative with much more potent activity, might serve as a candidate for anti-arthritis. The aim of the present study was to develop a sensitive and rapid high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for quantification of SND-117 in rat plasma and to understand its absolute bioavailability. The HPLC-MS/MS method was developed and fully validated for determination of SND-117 in rat plasma, and the pharmacokinetic differences were investigated after different administration routes. The pharmacokinetics parameters were calculated by non-compartment model with DAS 3.0 software. After the oral or intravenous administration of different doses of SND-117, the time to peak is 1.5h, half-life time is 8-10h. The absolute oral bioavailability of SND-117 in rats was 9.60%. The results showed that SND-117 in rats was quickly absorbed, slowly eliminate, and the kinetics were linear. This method was suitable for pharmacokinetic studies of SNA-117 in rats.
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Bei-Hua
authors.initials: BH
authors.lastname: Bao
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: An
authors.initials: A
authors.lastname: Kang
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Di
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China. Electronic address: lijxnju@nju.edu.cn.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Li
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D018501:Antirheumatic Agents / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D019540:Area Under Curve; D001172:Arthritis, Rheumatoid / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D009019:Morphinans / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D018501:Antirheumatic Agents; D009019:Morphinans; C009271:sinomenine
keywords: Anti-arthritis; HPLC–MS/MS; Pharmacokinetics; SND-117