title: Affinity binding of proteins to the modified bacterial cellulose nanofibers.
abstract: The potential of the modified bacterial cellulose (BC) nanofibers was determined bearing metal ion coordination interactions to enhance the protein adsorption and binding capacity. Thus, a household synthesized metal chelating monomer, namely N-methacryloyl-l-histidine methylester (MAH), and a commercial metal chelating monomer, namely 4-vinylimidazole (VIm), were used to complex with metal ions Cu(II) and Ni(II) respectively for the synthesis of the modified BC nanofibers. The modified nanofibers were characterized by FT-IR, SEM and EDX measurements. The protein adsorption tests were carried out using hemoglobin as a model protein and it was determined that the maximum adsorption capacity of hemoglobin onto the modified BC nanofibers was found as 47.40mg/g. The novel strategy for the preparation of metal chelated nanofibers was developed.
authors.affiliation: Hacettepe University, Chemistry Department, 06800 Ankara, Turkey.
authors.forename: Monireh
authors.initials: M
authors.lastname: Bakhshpour
authors.affiliation: Hacettepe University, Chemistry Department, 06800 Ankara, Turkey; Hitit University, Chemical Engineering Department, 19030 Çorum, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Tamahkar
authors.affiliation: Hacettepe University, Environmental Engineering Department, 06800 Ankara, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Andaç
authors.affiliation: Hacettepe University, Chemistry Department, 06800 Ankara, Turkey. Electronic address: denizli@hacettepe.edu.tr.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D002482:Cellulose / Q000031:analogs & derivatives*; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods; D003300:Copper / Q000737:chemistry*; D041822:Gluconacetobacter xylinus / Q000737:chemistry; D006454:Hemoglobins / Q000302:isolation & purification; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000031:analogs & derivatives*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D002614:Chelating Agents; D006454:Hemoglobins; D011135:Polysaccharides, Bacterial; D011506:Proteins; D003300:Copper; D009532:Nickel; D002482:Cellulose
keywords: Bacterial cellulose nanofibers; Metal ion coordination; Protein recognition