title: Selective Reaction Monitoring Mass Spectrometry for Quantitation of Glycolytic Enzymes in Postmortem Brain Samples.
abstract: Patients with psychiatric disorders exhibit dysfunctions in peripheral and central metabolism. This may be a root cause of impaired neuronal function, manifested as changes in mood, behavior, and cognitive capabilities in patients suffering with these conditions. Here we describe a selective reaction monitoring mass spectrometry (SRM-MS)-based targeted proteomic protocol for precise simultaneous quantitation of three glycolytic enzymes in postmortem brain tissue extracts. The SRM-MS approach has several advantages in terms of sensitivity, reproducibility, and reduced sample consumption, compared to traditional MS methods.
authors.affiliation: Department of Biochemistry and Immunology, RibeirãoPreto Medical School, University of São Paulo, RibeirãoPreto, SP, Brazil.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Lanfredi
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Guiherme
authors.initials: G
authors.lastname: Reis-de-Oliveira
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Veronica M
authors.initials: VM
authors.lastname: Saia-Cereda
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.|UNICAMP's Neurobiology Center, Campinas, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.affiliation: Department of Biochemistry and Immunology, RibeirãoPreto Medical School, University of São Paulo, RibeirãoPreto, SP, Brazil. vitor.faca@fmrp.usp.br.|Center for Cell Based Therapy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil. vitor.faca@fmrp.usp.br.
authors.forename: Vitor M
authors.initials: VM
authors.lastname: Faça
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000201:enzymology*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D006019:Glycolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D011180:Postmortem Changes; D014305:Triose-Phosphate Isomerase / Q000032:analysis*
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D010455:Peptides; D007770:L-Lactate Dehydrogenase; D010751:Phosphopyruvate Hydratase; D014305:Triose-Phosphate Isomerase
keywords: Mass spectrometry; Protein quantitation; Psychiatric disorders; Selective reaction monitoring; Targeted proteomics