title: New putative cryptic species detection and genetic network analysis of Bemisia tabaci (Hempitera: Aleyrodidae) in China based on mitochondrial COI sequences.
abstract: The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex and widely distributed throughout tropical and subtropical regions. To understand the B. tabaci cryptic species diversity in China more comprehensively, in the year 2014 and 2016, a large-scale sampling was conducted from the famous biodiversity hotspot of China, Yunnan province. Mitochondrial cytochrome oxidase I gene sequences were used to identify new putative cryptic species. Phylogenetic analyses were performed using Bayesian methods to evaluate the position of new cryptic species in the context of the B. tabaci diversity in Asia. Two new cryptic species, China 5 and Asia V were identified. In total, 19 B. tabaci cryptic species are present in China, two invasive (MED and MEAM1) and 17 indigenous. A new sibling species of B. tabaci was first defined and reported. Based on the mtCOI sequences and haplotype network analyses, the genetic diversity of MED was far higher than MEAM1. We confirmed the exotic MED was originated from the western Mediterranean regions and first invaded into Yunnan, China. The genetic structures of other four indigenous species (Asia I, Asia II 1, Asia II 6, and China 1) with relatively wide distribution ranges in China were also discussed.
authors.affiliation: a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
authors.affiliation: a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: b School of Biology and Chemistry, Pu'er University , Pu'er , China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Li
authors.affiliation: a Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation , Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences , Kunming , China.
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D044822:Biodiversity; D002681:China; D003576:Electron Transport Complex IV / Q000235:genetics*; D006430:Hemiptera / Q000145:classification* / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D008522:Mediterranean Sea; D010802:Phylogeny; D058974:Phylogeography; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D019476:Insect Proteins; D003576:Electron Transport Complex IV
keywords: Bemisia tabaci; cryptic species; haplotype network; mitochondrial COI; phylogenetic tree