title: The combined effects of elevated pCO2and food availability on Tigriopus japonicus Mori larval development, reproduction, and superoxide dismutase activity.
abstract: Previous studies have shown that ocean acidification has little effect on adult Tigriopus japonicus copepods, and mainly impairs the early development and reproduction of females. This study investigated the possible interactive effect between CO2-induced seawater acidification and food availability on larval development and reproductive output in T. japonicus. Copepods were exposed to either pH8.1 or pH7.3 under different food concentrations (0.5×104-80.0×104cells/mL). Both the development of nauplii and copepodites was delayed at pH7.3 with a greater effect at lower food concentrations. The reproductive output followed a bell-shaped curve with the highest reproductive output at food concentrations between 30×104and 40×104cells/mL. As an indicator of oxidative stress, the activity of superoxide dismutase increased at lower pH, with a greater increase at lower food concentrations. Therefore, the effect of elevated pCO2on T. japonicus was food dependent.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China.
authors.forename: S G
authors.initials: SG
authors.lastname: Cheung
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China.
authors.forename: P K S
authors.initials: PKS
authors.lastname: Shin
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China.
authors.forename: Xiaoshou
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China. Electronic address: fanghongmu@ouc.edu.cn.
authors.forename: Fanghong
authors.initials: F
authors.lastname: Mu
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis*; D033342:Copepoda / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D005260:Female; D005502:Food; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012098:Reproduction; D012623:Seawater; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002245:Carbon Dioxide; D013482:Superoxide Dismutase
keywords: Development; Food concentration; Reproduction; SOD activity; Seawater acidification; Tigriopus japonicus