title: Effects of TiO2crystal structure on the luminescence quenching of [Ru(bpy)2(dppz)]2+-intercalated into DNA.
abstract: The intercalation of [Ru(bpy)2(dppz)]2+labeled as Ru(II) (bpy=2,2'-bipyridine and dppz=dipyrido[3,2,-a:2',3'-c]phenazine) into herring sperm DNA leads to the formation of emissive Ru(II)-DNA dyads, which can be quenched by TiO2nanoparticles (NPs) and sol-gel silica matrices at heterogeneous interfaces. The calcinations temperature exhibits a remarkable influence on the luminescence quenching of the Ru(II)-DNA dyads by TiO2NPs. With increasing calcinations temperature in the range from 200 to 850°C, the anatase-to-rutile TiO2crystal structure transformation increases the average particle size and hydrodynamic diameter of TiO2and DNA@TiO2. The anatase TiO2has the stronger ability to unbind the Ru(II)-DNA dyads than the rutile TiO2at room temperature. The TiO2NPs and sol-gel silica matrices can quench the luminescence of the Ru(II) complex intercalated into DNA by selectively capturing the negatively DNA and positively charged Ru(II) complex to unbind the dyads, respectively. This present results provide new insights into the luminescence quenching and competitive binding of dye-labeled DNA dyads by inorganic NPs.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Minggao
authors.initials: M
authors.lastname: Huang
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors.forename: Licai
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China. Electronic address: lihong@scnu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009942:Organometallic Compounds / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry*; D013696:Temperature; D014025:Titanium / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D010619:Phenazines; C009495:titanium dioxide; C077849:bis(2,2'-bipyridyl)(dipyrido(3,2-alpha-2',3'-c)phenazine)ruthenium (II); D004247:DNA; D014025:Titanium
keywords: Binding; DNA; Luminescence quenching; Polypyridyl ruthenium(II) complex; Titanium dioxide nanoparticle