title: Outbreak investigations and molecular characterization of foot-and-mouth disease viruses circulating in south-west Niger.
abstract: In Niger, the epidemiological situation regarding foot-and-mouth disease is unclear as many outbreaks are unreported. This study aimed (i) to identify Foot-and-mouth disease virus (FMDV) strains currently circulating in cattle herds, and (ii) to identify risk factors associated with Foot-and-mouth disease (FMD)-seropositive animals in clinical outbreaks. Epithelial tissues (n = 25) and sera (n = 227) were collected from cattle in eight districts of the south-western part of Niger. Testing of clinical material revealed the presence of FMDV serotype O that was characterized within the O/WEST AFRICA topotype. The antigenic relationship between one of the FMDV isolates from Niger (O/NGR/4/2015) and three reference vaccine strains was determined by the two-dimensional virus neutralization test (2dmVNT), revealing a close antigenic match between the field isolate from Niger and three FMDV serotype O vaccine strains. Serological analyses using a non-structural protein (NSP) test provided evidence for previous FMDV infection in 70% (158/227) of the sera tested. Multivariate logistic regression analysis revealed that only the herd composition (presence of both cattle and small ruminants) was significantly associated with FMDV seropositivity as defined by NSP-positive results (p-value = .006). Of these positive sera, subsequent testing by liquid-phase blocking ELISA (LPBE) showed that 86% (136/158) were positive for one (or more) of four FMDV serotypes (A, O, Southern African Territories (SAT) 1 and SAT 2). This study provides epidemiological information about FMD in the south-western part of Niger and highlights the complex transboundary nature of FMD in Africa. These findings may help to develop effective control and preventive strategies for FMD in Niger as well, as other countries in West Africa.
authors.affiliation: Faculty of Veterinary Medicine, Research Unit in Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH) Centre, University of Liege, Liege, Belgium.|Institut National de la Recherche Agronomique du Niger (INRAN), Niamey, Niger.
authors.forename: B
authors.initials: B
authors.lastname: Souley Kouato
authors.affiliation: Botswana Vaccine Institute, Gaborone, Botswana.
authors.forename: F M
authors.initials: FM
authors.lastname: Elliot
authors.affiliation: The Pirbright Institute, Woking, Surrey, UK.
authors.forename: D P
authors.initials: DP
authors.lastname: King
authors.affiliation: Botswana Vaccine Institute, Gaborone, Botswana.
authors.forename: J
authors.initials: J
authors.lastname: Hyera
authors.affiliation: The Pirbright Institute, Woking, Surrey, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Knowles
authors.affiliation: The Pirbright Institute, Woking, Surrey, UK.
authors.forename: A B
authors.initials: AB
authors.lastname: Ludi
authors.affiliation: The Pirbright Institute, Woking, Surrey, UK.
authors.forename: V
authors.initials: V
authors.lastname: Mioulet
authors.affiliation: Botswana Vaccine Institute, Gaborone, Botswana.
authors.forename: G
authors.initials: G
authors.lastname: Matlho
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Brussels, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: De Clercq
authors.affiliation: Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Thys
authors.affiliation: Faculté d'Agronomie, Université Abdou Moumouni de Niamey, Niamey, Niger.
authors.forename: H
authors.initials: H
authors.lastname: Marichatou
authors.affiliation: Institut National de la Recherche Agronomique du Niger (INRAN), Niamey, Niger.
authors.forename: S
authors.initials: S
authors.lastname: Issa
authors.affiliation: Faculty of Veterinary Medicine, Research Unit in Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH) Centre, University of Liege, Liege, Belgium.
authors.forename: C
authors.identifier: http://orcid.org/0000-0001-9087-7436
authors.initials: C
authors.lastname: Saegerman
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000453:epidemiology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009548:Niger / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D065288:Serogroup
keywords: foot-and-mouth disease virus; identification; molecular characterization; risk factors; serology; south-western Niger