title: Unliganded and substrate bound structures of the cellooligosaccharide active lytic polysaccharide monooxygenase LsAA9A at low pH.
abstract: Lytic polysaccharide monooxygenases (LPMOs) have been found to be key components in microbial (bacterial and fungal) degradation of biomass. They are copper metalloenzymes that degrade polysaccharides oxidatively and act in synergy with glycoside hydrolases. Recently crystallographic studies carried out at pH 5.5 of the LPMO from Lentinus similis belonging to the fungal LPMO family AA9 have provided the first atomic resolution view of substrate-LPMO interactions. The LsAA9A structure presented here determined at pH 3.5 shows significant disorder of the active site in the absence of substrate ligand. Furthermore some differences are also observed in regards to substrate (cellohexaose) binding, although the major interaction with the N-terminal histidine remains unchanged.
authors.affiliation: Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
authors.forename: Kristian E H
authors.initials: KEH
authors.lastname: Frandsen
authors.affiliation: Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
authors.forename: Jens-Christian N
authors.initials: JN
authors.lastname: Poulsen
authors.affiliation: Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
authors.forename: Tobias
authors.initials: T
authors.lastname: Tandrup
authors.affiliation: Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark. Electronic address: leila@chem.ku.dk.
authors.forename: Leila
authors.initials: L
authors.lastname: Lo Leggio
mesh_terms: D020134:Catalytic Domain; D006863:Hydrogen-Ion Concentration; D020074:Lentinula / Q000201:enzymology; D008024:Ligands; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D008024:Ligands; D009844:Oligosaccharides; C099423:cellohexaose; D006899:Mixed Function Oxygenases
keywords: Cellohexaose interactions; Lytic polysaccharide monooxygenases; pH-dependent disorder