title: Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals.
abstract: Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collected via neutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, 987696 Nebraska Medical Center, Omaha, NE 68198-7696, USA.
authors.forename: Jahaun
authors.initials: J
authors.lastname: Azadmanesh
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, 987696 Nebraska Medical Center, Omaha, NE 68198-7696, USA.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Trickel
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831, USA.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Weiss
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831, USA.
authors.forename: Leighton
authors.initials: L
authors.lastname: Coates
authors.affiliation: Eppley Institute for Research in Cancer and Allied Diseases, 987696 Nebraska Medical Center, Omaha, NE 68198-7696, USA.
authors.forename: Gloria E O
authors.initials: GE
authors.lastname: Borgstahl
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D003461:Crystallography; D017666:Deuterium Oxide / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D033363:Neutron Diffraction; D011522:Protons; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011522:Protons; D011994:Recombinant Proteins; D013482:Superoxide Dismutase; D017666:Deuterium Oxide
keywords: human; large unit cell; manganese superoxide dismutase; neutron diffraction; perdeuteration