title: Upregulation of SENP3/SMT3IP1 promotes epithelial ovarian cancer progression and forecasts poor prognosis.
abstract: As a crucial member of the small ubiquitin-like modifier system, SUMO-specific protease 3, was identified to be essential for cell proliferation and ribosomal RNA processing. Recent studies showed that SUMO-specific protease 3 was elevated in ovarian cancer compared to normal tissue samples. However, the connection between SUMO-specific protease 3-specific expression and clinicopathological parameters of epithelial ovarian cancer, as well as the physiologically potential role of SUMO-specific protease 3 in epithelial ovarian cancer remained unclear. In this study, an analysis of 124 paraffin-embedded slices by immunohistochemistry indicated that SUMO-specific protease 3 expression was positively correlated with the International Federation of Gynecology and Obstetrics stages (p = 0.025), tumor grade (p = 0.004), and lymph node metastasis (p = 0.001) and was also a critical prognostic factor for the overall survival of epithelial ovarian cancer patients, as revealed by Kaplan-Meier curve analysis. Knockdown of SUMO-specific protease 3 weakened the proliferation, migration, and invasion capability of ovarian cancer cells, down-regulated the expression of Proliferating Cell Nuclear Antigen, Forkhead Box C2, and N-cadherin, and resulted in upregulation of p21 and E-cadherin. Consistent with our results, SUMO-specific protease 3 had been verified to promote cell proliferation, metastasis, and tumorigenesis in multiple malignant cancers, which was a redox-sensitive molecule mediating the epithelial-mesenchymal transition. Collectively, our findings for the first time specifically supported that SUMO-specific protease 3 might play an important role in the regulation of epithelial ovarian cancer progression and could serve as a potential biomarker for prognosis as well as provide a promising therapeutic target against epithelial ovarian cancer.
authors.affiliation: 1 Department of Oncology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: 2 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.affiliation: 2 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Jin
authors.affiliation: 2 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xi
authors.affiliation: 2 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: 1 Department of Oncology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.|3 Department of Oncology, Jiangyin People's Hospital, Wuxi, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: 2 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: 2 Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 4 Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.affiliation: 5 Department of Radiology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Shi
authors.affiliation: 1 Department of Oncology, Affiliated Hospital of Nantong University, Nantong University, Nantong, People's Republic of China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Mao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D003546:Cysteine Endopeptidases; C499718:SENP3 protein, human
keywords: Epithelial ovarian cancer; SUMO-specific protease 3; cancer progression; prognosis