title: Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.
abstract: The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast, S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments.
authors.affiliation: Department of Molecular Biology and Biochemistry, Wesleyan University, 45 Wyllys Avenue, Middletown, CT, 06459, USA. amacqueen@wesleyan.edu.
authors.forename: Amy J
authors.initials: AJ
authors.lastname: MacQueen
authors.affiliation: Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California-Berkeley, Berkeley, CA, USA.
authors.forename: Beth
authors.initials: B
authors.lastname: Rockmill
mesh_terms: D015825:Chromosomes, Fungal; D004958:Estradiol / Q000494:pharmacology; D005455:Fluorescent Antibody Technique / Q000379:methods; D008540:Meiosis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004958:Estradiol
keywords: Chromatin; Conditional expression; Meiosis; Synaptonemal complex; Yeast