title: Human leptospirosis: occurrence of serovars of Leptospira spp. in the state of Minas Gerais, Brazil, from 2008 to 2012.
abstract: BACKGROUND
  Leptospirosis is an infectious and acute disease caused by Leptospira spp. that have high epidemic potential. This study verified the main Leptospira spp. serovars detected by MAT from serum of patients with suspicion of leptospirosis from 2008 to 2012 in Minas Gerais State.
  
  
  METHODS
  The laboratory received sera from 4654 patients. All serum were screened by IgM-ELISA according to the manufacturer's instructions. Each sample reactive or indeterminate were tested against twenty-four serovars of Leptospira by MAT.
  
  
  RESULTS
  In this study, 597 patients were classified as reactive on MAT. Only 301 patients were confirmed by laboratory test. It was not possible confirmation by laboratory diagnosis of 296 patients. Among the samples classified as reactive on MAT, 273 patients exhibited titers bigger than 800 for one or more serovars; seroconversion was detected in 28 cases. Percentage of 85.1% of the samples reactive on MAT corresponded to males, 39.4% corresponded to patients aged between 20 and 39 years old. The most common serovars found were Icterohaemorrhagiae, Andamana, Patoc, Tarassovi, Copenhageni, Hardjo and Australis. Concerning the samples that exhibited titers bigger than 800, serovar Icterohaemorrhagiae was also the most common, followed by Copenhageni, Andamana, Patoc, Tarassovi, Grippotyphosa and Canicola. In this study, 40% of the cases occurred to the metropolitan area, state capital and 34 neighboring towns.
  
  
  CONCLUSIONS
  Our results show the possibly spreading serovars in Minas Gerais State and contribute to knowledge of human leptospirosis, aiming at improving the prevention, control of the disease, as well as the treatment of infected patients.
authors.affiliation: Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil. Electronic address: marluce.oliveira@funed.mg.gov.br.
authors.forename: Marluce Aparecida Assunção
authors.initials: MAA
authors.lastname: Oliveira
authors.affiliation: Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
authors.forename: Élida Aparecida
authors.initials: ÉA
authors.lastname: Leal
authors.affiliation: Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
authors.forename: Max Assunção
authors.initials: MA
authors.lastname: Correia
authors.affiliation: Universidade Federal de Minas Gerais, Faculdade de Medicina, Belo Horizonte, MG, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Serufo Filho
authors.affiliation: Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
authors.forename: Ricardo Souza
authors.initials: RS
authors.lastname: Dias
authors.affiliation: Universidade Federal de Minas Gerais, Faculdade de Medicina, Belo Horizonte, MG, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Serufo
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D005260:Female; D006801:Humans; D007919:Leptospira / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007922:Leptospirosis / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology*; D008297:Male; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial
keywords: Epidemiology; Leptospira; MAT; Seroprevalence; Serovar