title: MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer.
abstract: We intended to investigate the functional role and clinical relevance of microRNA-125b in human gallbladder cancer. Quantitative real-time polymerase chain reaction was used to examine microRNA-125b expression in gallbladder cancer cell lines, and 79 pairs of gallbladder cancer and normal gallbladder clinical tissues. Clinical correlations between tumorous microRNA-125b expression and gallbladder cancer patients' clinicopathological variances or overall survivals were statistically analyzed. In gallbladder cancer cell lines, TYGBK-8 and G-415 cells, microRNA-125b was upregulated to examine its regulatory effect on gallbladder cancer proliferation and migration in vitro. MicroRNA-125b was significantly downregulated in gallbladder cancer cell lines and human gallbladder cancer tumors. MicroRNA-125b in gallbladder cancer was significantly correlated with patients' clinical stage, tumor differentiation, lymph metastasis, and tumor invasion. Low tumorous microRNA-125b expression was also found to be associated with poor overall survivals among gallbladder cancer patients. In vitro studies demonstrated that microRNA-125b upregulation significantly suppressed proliferation and migration in TYGBK-8 and G-415 cells. Tumorous microRNA-125b is an independent prognostic biomarker for patients with gallbladder cancer and possibly acts as a tumor suppressor in gallbladder cancer.
authors.affiliation: 1 Radiation Center, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.|Co-first authors.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: 2 Department of General Surgery, Fuzhou General Hospital, Fuzhou, China.|Co-first authors.
authors.forename: Sheng
authors.initials: S
authors.lastname: Huang
authors.affiliation: 1 Radiation Center, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.|Co-first authors.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Ma
authors.affiliation: 1 Radiation Center, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.|3 Department of Integrated Traditional Chinese and Western Medicine, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Wu
authors.affiliation: 1 Radiation Center, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.|4 Department of Biliary Tract, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Feiling
authors.initials: F
authors.lastname: Feng
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002763:Cholecystectomy; D015536:Down-Regulation; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D011237:Predictive Value of Tests; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs
keywords: Gallbladder cancer; clinic relevance; microRNA-125b; overall survival; proliferation