title: Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum.
abstract: Mycoplasma capricolum subsp. capricolum is one of the causative agents of contagious agalactia (CA). Nevertheless, there is still a lack of information about its antimicrobial susceptibility and genetic characteristics. Therefore, the aim of this work was to study the antimicrobial and genetic variability of different Mycoplasma capricolum subsp. capricolum field isolates. For this purpose, the growth inhibition effect of 18 antimicrobials and a multilocus sequence typing (MLST) scheme based on five housekeeping genes (fusA, glpQ, gyrB, lepA and rpoB) were performed on 32 selected field isolates from Italy and Spain.The results showed a wide range of growth inhibitory effects for almost all the antimicrobials studied. Macrolides presented lower efficacy inhibiting Mcc growth than in previous works performed on other CA-causative mycoplasmas. Erythromycin was not able to inhibit the growth of any of the studied strains, contrary to doxycycline, which inhibited the growth of all of them from low concentrations. On the other hand, the study of the concatenated genes revealed a high genetic variability among the different Mcc isolates. Hence, these genetic variations were greater than the ones reported in prior works on other mycoplasma species.
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Tatay-Dualde
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Miranda
authors.initials: M
authors.lastname: Prats-van der Ham
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Christian
authors.identifier: http://orcid.org/0000-0002-4354-1689
authors.initials: C
authors.lastname: de la Fe
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Paterna
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Sánchez
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Corrales
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Contreras
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", Sassari, Italy.
authors.forename: Sebastiana
authors.initials: S
authors.lastname: Tola
authors.affiliation: Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo s/n., Murcia, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gómez-Martin
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D014644:Genetic Variation; D007558:Italy; D018942:Macrolides / Q000494:pharmacology*; D058885:Multilocus Sequence Typing; D045623:Mycoplasma capricolum / Q000187:drug effects* / Q000235:genetics; D013030:Spain
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D018942:Macrolides