title: Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay.
abstract: Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk. The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL. The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively. For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0.30 ng/mL, and 0.50 ng/mL), the actual detection value was 0.0996, 0.3096, and 0.4905, respectively. The recovery rate of AFM1 was 99.7%-101.7%, while that for CAP was 95.3%-97.6%. For the test stability, AFM1 and CAP showed satisfactory test stability even at month 5. Compared with the sensitivity of liquid chromatography-mass spectrometry (LC-MS) method, no statistical difference was noticed in results of the bFQICA. Our method is convenient for the detection of AFM1 and CAP in milk with a test duration of about 8 minutes. Additionally, an internal WiFi facility is provided in the system allowing for quick connection and storage in the intelligent cell phone.
authors.affiliation: College of Pharmacy, Xinjiang Medical University, Urumqi 830000, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wu
authors.affiliation: Shanghai Simp Bio-Science Co., Ltd., Shanghai 201800, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Tian
authors.affiliation: Center for Disease Control and Prevention, Xinjiang Uyghur Autonomous Region, Urumqi 830000, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xu
authors.affiliation: Shanghai Simp Bio-Science Co., Ltd., Shanghai 201800, China.
authors.forename: Jiutong
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Pharmacy, Xinjiang Medical University, Urumqi 830000, China.
authors.forename: Xinxia
authors.identifier: 0000-0002-6860-5909
authors.initials: X
authors.lastname: Li
authors.affiliation: Center for Disease Control and Prevention, Xinjiang Uyghur Autonomous Region, Urumqi 830000, China.
authors.forename: Yuwen
authors.identifier: 0000-0001-5456-4168
authors.initials: Y
authors.lastname: Wang
mesh_terms: D016607:Aflatoxin M1 / Q000302:isolation & purification* / Q000633:toxicity; D000818:Animals; D002701:Chloramphenicol / Q000302:isolation & purification* / Q000633:toxicity; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry* / Q000633:toxicity
chemical_list: D002701:Chloramphenicol; D016607:Aflatoxin M1