title: Inhibitory Effects of Thai Essential Oils on Potentially Aflatoxigenic Aspergillus parasiticus and Aspergillus flavus.
abstract: The antiaflatoxigenic and antifungal activities of essential oils (EOs) of finger root (Boesenbergia rotunda (L.) Mansf.), pine (Pinus pinaster), rosewood (Aniba rosaedora), Siam benzoin (Styrax tonkinensis), Thai moringa (Moringa oleifera), and ylang ylang (Cananga odorata) were tested for Aspergillus parasiticus and Aspergillus flavus in potato dextrose broth. Aflatoxin B1(AFB1) was extracted from culture using a QuEChERS-based extraction procedure and analyzed with high performance liquid chromatography (HPLC) coupled to a fluorescence detector. EO of pine showed the greatest inhibition of growth and AFB1production of A. parasiticus, followed by EOs of rosewood, finger root, Siam benzoin, and ylang ylang. EO of finger root gave the best inhibitory effects on A. flavus, followed by EOs of rosewood, pine, ylang ylang, and Siam benzoin. EO of Thai moringa did not show any significant inhibition of aflatoxigenic fungi. The antiaflatoxigenic activities of EOs correlated with their antifungal activities in the dosedependent manner. Comparison of the application of the five selected EOs in peanut pods by direct and vapor exposure indicated that the AFB1production inhibitory effects of the five EOs by direct exposure were faster and more effective than by vapor exposure. EO of finger root showed the best inhibition of AFB1production of A. flavus in peanut pods by direct exposure, followed by EOs of pine, rosewood, ylang ylang, and Siam benzoin.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University.
authors.forename: Kittika
authors.initials: K
authors.lastname: Jantapan
authors.forename: Amnart
authors.initials: A
authors.lastname: Poapolathep
authors.forename: Kanjana
authors.initials: K
authors.lastname: Imsilp
authors.forename: Saranya
authors.initials: S
authors.lastname: Poapolathep
authors.forename: Phanwimol
authors.initials: P
authors.lastname: Tanhan
authors.forename: Susumu
authors.initials: S
authors.lastname: Kumagai
authors.forename: Usuma
authors.initials: U
authors.lastname: Jermnak
mesh_terms: D016604:Aflatoxin B1 / Q000096:biosynthesis; D000935:Antifungal Agents / Q000494:pharmacology*; D010367:Arachis / Q000382:microbiology; D001230:Aspergillus / Q000187:drug effects* / Q000378:metabolism; D001231:Aspergillus flavus / Q000187:drug effects* / Q000378:metabolism; D003470:Culture Media; D009822:Oils, Volatile / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D009822:Oils, Volatile; D016604:Aflatoxin B1