title: Synthesis of β-cyclodextrin-lysozyme conjugates and their physicochemical and biochemical properties.
abstract: Recently a great interest in the field of protein engineering and the design of innovative drug delivery systems employing specific ligands such as cyclodextrins is observed. The paper reports the solid state, thermal method for protein coupling with β-cyclodextrin and the physicochemical and biological properties of the obtained conjugates. The structure of the obtained conjugates was investigated via liquid chromatography-mass spectrometry, dynamic light scattering and circular dichroism analysis. The presented conjugates were biologically active and covalently bound β-cyclodextrin preserved the ability to form inclusion complexes with the model compound. This report demonstrates the great potential of cyclodextrin as a modifying unit that can be used to modulate the properties of therapeutic proteins, additionally giving such conjugates the possibility to transport many therapeutic substances in the form of inclusion complexes. In addition, the paper presents the potential of protein-cyclodextrin conjugates to construct innovative bioactive molecules for biological and medical applications.
authors.affiliation: Laboratory of Biomedical Chemistry, Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 12 Rudolf Weigl St., 53-114 Wrocław, Poland.
authors.forename: Tomasz Marek
authors.initials: TM
authors.lastname: Goszczyński
authors.affiliation: Laboratory of Biomedical Chemistry, Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 12 Rudolf Weigl St., 53-114 Wrocław, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Gawłowski
authors.affiliation: Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University, Armii Krajowej Ave., 13/15, 42 201 Częstochowa, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Girek
authors.affiliation: Laboratory of Biomedical Chemistry, Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 12 Rudolf Weigl St., 53-114 Wrocław, Poland.
authors.forename: Konrad
authors.initials: K
authors.lastname: Kowalski
authors.affiliation: Laboratory of Biomedical Chemistry, Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, 12 Rudolf Weigl St., 53-114 Wrocław, Poland.|Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University, Armii Krajowej Ave., 13/15, 42 201 Częstochowa, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Boratyński
authors.affiliation: Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University, Armii Krajowej Ave., 13/15, 42 201 Częstochowa, Poland.
authors.forename: Tomasz
authors.identifier: 0000-0001-6647-7168
authors.initials: T
authors.lastname: Girek
keywords: Conjugates; Cyclodextrin; Inclusion complex; Protein; Thermal reaction in solid state