title: Identification of ace inhibitory cryptides in Tilapia protein hydrolysate by UPLC-MS/MS coupled to database analysis.
abstract: An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry method was developed and applied to identify short angiotensin-I-converting enzyme (ACE) inhibitory cryptides in Tilapia (Oreochromis Niloticus) protein hydrolyzate. A database was created with previously identified ACE-inhibitory di- and tripeptides and the lowest molecular weight fraction of Tilapia hydrolysate was analysed for coincidences. Only VW and VY were identified. Further analysis of collected fractions conducted to the identification of 51 different peptides in major fractions. 19 peptides selected were synthesised and tested for their ACE inhibitory potential. TL, TI, IK, LR, LD, IQ, DI, AILE, ALLE, ALIE and AIIE were identified as new ACE inhibitors. The findings from this study point UPLC-MS/MS combined with the creation of a database as an efficient technique to identify specific short peptides within a complex hydrolysate, in addition with de novo sequencing. This efficient characterisation of bioactive factors like cryptides in protein hydrolysates will extend their use as functional foods.
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France.
authors.forename: Ben Henda
authors.initials: BH
authors.lastname: Yesmine
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France; Platform for the High Resolution Analysis of Biomolecules, University of La Rochelle, UMR CNRS 7266 LIENSs, 17042 La Rochelle, France.
authors.forename: Bonnet
authors.initials: B
authors.lastname: Antoine
authors.affiliation: UNIOESTE- campus Toledo, Rua da Faculdade, 645 - Jardim La Salle, Toledo, CEP 85903-000, Paraná, Brazil.
authors.forename: Nunes Gonzalez
authors.initials: NG
authors.lastname: da Silva Ortência Leocádia
authors.affiliation: UNIOESTE- campus Toledo, Rua da Faculdade, 645 - Jardim La Salle, Toledo, CEP 85903-000, Paraná, Brazil.
authors.forename: Boscolo Wilson
authors.initials: BW
authors.lastname: Rogério
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France.
authors.forename: Arnaudin
authors.initials: A
authors.lastname: Ingrid
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France.
authors.forename: Bridiau
authors.initials: B
authors.lastname: Nicolas
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France.
authors.forename: Maugard
authors.initials: M
authors.lastname: Thierry
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France.
authors.forename: Piot
authors.initials: P
authors.lastname: Jean-Marie
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France.
authors.forename: Sannier
authors.initials: S
authors.lastname: Frédéric
authors.affiliation: Université de La Rochelle, Laboratoire LIENSs UMR CNRS-7266, Approches Moléculaires, Environnement Santé, site Marie Curie, Faculté des Sciences et Technologies, BP 33060, F-17031 La Rochelle, France. Electronic address: sbordena@univ-lr.fr.
authors.forename: Bordenave-Juchereau
authors.initials: BJ
authors.lastname: Stéphanie
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011817:Rabbits; D053719:Tandem Mass Spectrometry; D017210:Tilapia / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D029941:Fish Proteins; D010455:Peptides; D007703:Peptidyl-Dipeptidase A
keywords: ACE; Cryptides; Database; MS/MS; Tilapia protein hydrolysate; UPLC