title: Investigation of repressive and enhancive effects of fruit extracts on the activity of glucose-6-phophatase.
abstract: Glucose-6-phosphatase is a key enzyme of glucose metabolic pathways. Deficiency of this enzyme leads to glycogen storage disease. This enzyme also plays a negative role in diabetes mellitus disorder in which the catalytic activity of this enzyme increases. Thus there is need for activators to enhance the activity of glucose-6-phosphatase in glycogen storage disease of type 1b while in diabetes mellitus repressors are needed to reduce its activity. Crude extracts of apricot, fig, mulberry and apple fruits were investigated for their repressive/enhancive effects on glucose-6-phosphatase in vivo. Albino mice were used as experimental animal. All the selected extracts showed depressive effects on glucose-6-phosphatase, which shows that all these extracts can be used as antidiabetic supplement of food. The inhibitory pattern was competitive one, which was evident from the effect of increasing dose from 1g/Kg body weight to 3g/Kg body weight for all the selected fruit extracts. However fig and apple fruit extracts showed high repressive effects for high doses as compared to apricot and mulberry fruit extracts. None of these selected fruit extracts showed enhancive effect on glucose-6-phosphatase activity. All these fruits or their extracts can be used as antidiabetic dietary supplement for diabetes mellitus.
authors.affiliation: Department of Chemistry, University of Malakand, Chakdara Dir (Lower), KPK, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zahoor
authors.affiliation: Department of Chemistry, University of Malakand, Chakdara Dir (Lower), KPK, Pakistan.
authors.forename: Muhammad Rasul
authors.initials: MR
authors.lastname: Jan
authors.affiliation: Department of Chemistry, University of Malakand, Chakdara Dir (Lower), KPK, Pakistan.
authors.forename: Sumaira
authors.initials: S
authors.lastname: Naz
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D020536:Enzyme Activators / Q000302:isolation & purification / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D030681:Ficus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D027845:Malus / Q000737:chemistry*; D051379:Mice; D031625:Morus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D000068219:Prunus armeniaca / Q000737:chemistry*
chemical_list: D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D005952:Glucose-6-Phosphatase