title: Impact of sulfadiazine on performance and microbial community of a sequencing batch biofilm reactor treating synthetic mariculture wastewater.
abstract: The impact of sulfadiazine on the performance, microbial activity and microbial community of a sequencing batch biofilm reactor (SBBR) were evaluated in treating mariculture wastewater due to the application of sulfadiazine as an antibiotic in mariculture. The COD and nitrogen removals kept stable at 0-6mg/L sulfadiazine and were inhibited at 10-35mg/L sulfadiazine. The microbial activities related to organic matter and nitrogen removals reduced with an increase in sulfadiazine concentration. The presence of sulfadiazine could affect the production and chemical composition of loosely bound extracellular polymeric substances (LB-EPS) and tightly bound EPS (TB-EPS) in the biofilm. High-throughput sequencing demonstrated that sulfadiazine could impact on the microbial richness and diversity of SBBR treating mariculture wastewater. The relative abundances of Nitrosomonas, Nitrospira, Paracoccus, Hyphomicrobium, Rhodanobacter, Thauera and Steroidobacter decreased with an increase in sulfadiazine concentration, indicating that the presence of sulfadiazine decreased the relative abundance of some nitrifying and denitrifying bacteria.
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, PR China. Electronic address: mengchungao@outlook.com.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Yangguo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Chunji
authors.initials: C
authors.lastname: Jin
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Xu
mesh_terms: D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology; D009584:Nitrogen / Q000378:metabolism; D013411:Sulfadiazine; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*
chemical_list: D009584:Nitrogen; D013411:Sulfadiazine; D062065:Wastewater
keywords: Extracellular polymeric substances; Mariculture wastewater; Microbial community; SBBR; Sulfadiazine