title: Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge.
abstract: Antibody-secreting cells are generated in regional lymphoid tissues and traffic as plasmablasts (PBs) via lymph and blood to target sites for local immunity. We used multiparameter flow cytometry to define PB trafficking programs (TPs, combinations of adhesion molecules and chemoattractant receptors) and their imprinting in patients in response to localized infection or immune insults. TPs enriched after infection or autoimmune inflammation of mucosae correlate with sites of immune response or symptoms, with different TPs imprinted during small intestinal, colon, throat, and upper respiratory immune challenge. PBs induced after intramuscular or intradermal influenza vaccination, including flu-specific antibody-secreting cells, display TPs characterized by the lack of mucosal homing receptors. PBs of healthy donors display diverse mucosa-associated TPs, consistent with homeostatic immune activity. Identification of TP signatures of PBs may facilitate noninvasive monitoring of organ-specific immune responses.
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, California, USA and the Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.|Program of Immunology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Yekyung
authors.initials: Y
authors.lastname: Seong
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, California, USA and the Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.|The Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.
authors.forename: Nicole H
authors.initials: NH
authors.lastname: Lazarus
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, California, USA and the Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.|The Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.
authors.forename: Lusijah
authors.initials: L
authors.lastname: Sutherland
authors.affiliation: Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Aida
authors.initials: A
authors.lastname: Habtezion
authors.affiliation: San Jose State University, Department of Biology, San Jose, California, USA.
authors.forename: Tzvia
authors.initials: T
authors.lastname: Abramson
authors.affiliation: The Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.|Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: He
authors.affiliation: The Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.|Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.|Department of Immunology and Microbiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Harry B
authors.initials: HB
authors.lastname: Greenberg
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, California, USA and the Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.|The Center for Molecular Biology and Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, California, USA.
authors.forename: Eugene C
authors.initials: EC
authors.lastname: Butcher
mesh_terms: D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology*; D001692:Biological Transport; D002446:Celiac Disease / Q000276:immunology; D003093:Colitis, Ulcerative / Q000276:immunology; D006801:Humans; D018928:Immunity, Mucosal; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology