title: Plasma-treated water eliminates Streptococcus mutans in infected dentin model.
abstract: Non-mechanical procedures for removing caries-infected dentin are warranted in dentistry. We previously demonstrated the marked sterilization effect for direct irradiation of low-temperature plasma using dentin model infected with Streptococcus mutans. However it requires 180 s of intraoral plasma irradiation to eliminate bacteria. We alternatively investigated whether plasma-treated water (PTW), i.e., pure water exposed to plasma in an atmosphere, has a same bactericidal activity with the plasma irradiation. In the infected dentin model, the viable S. mutans counts recovered by bur at depth of 0.8-2.4 mm from the cavity floor were 104-106CFU/round bur. After PTW application for only 10 s, the count was significantly decreased to below the detection limit (2.5 CFU/round bur) or 3.0±5.0 CFU/round bur. Since the bactericidal activity of PTW is rapidly deactivated at body temperature (37°C), PTW is likely to be biocompatible and holds significant potential for non-mechanical procedures for removing caries-infected dentin.
authors.affiliation: Department of Operative Dentistry, Tsurumi University School of Dental Medicine.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tasaki
authors.affiliation: Department of Oral Microbiology, Tsurumi University School of Dental Medicine.|Center for Atomic and Molecular Technologies, Graduate School of Engineering, Osaka Univirsity.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ohshima
authors.affiliation: Department of Operative Dentistry, Tsurumi University School of Dental Medicine.
authors.forename: Emi
authors.initials: E
authors.lastname: Usui
authors.affiliation: Technology Research Institute of Osaka Prefecture.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ikawa
authors.affiliation: Department of Oral Microbiology, Tsurumi University School of Dental Medicine.|Center for Atomic and Molecular Technologies, Graduate School of Engineering, Osaka Univirsity.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kitano
authors.affiliation: Department of Oral Microbiology, Tsurumi University School of Dental Medicine.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Maeda
authors.affiliation: Department of Operative Dentistry, Tsurumi University School of Dental Medicine.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Momoi
mesh_terms: D003731:Dental Caries; D003786:Dental Pulp Cavity; D003804:Dentin; D013295:Streptococcus mutans; D014867:Water
chemical_list: D014867:Water
keywords: Dentin; Plasma; Sterilization; Streptococcus mutans; Water