title: Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice.
abstract: Obesity is a major public health issue, with a prevalence of 4 to 28% for men and 6.2 to 36.5% for women in Europe (from 2003 to 2008). Morbid obesity is frequently associated with metabolic complications, such as type 2 diabetes, hypertension, and dyslipidemia, reducing life expectancy and quality. In the absence of any effective noninvasive treatments, bariatric surgery is a valuable therapeutic option for patients with morbid obesity (body mass index (BMI) >40 kg/m2), leading to long-term, sustained weight loss and improvements in metabolic complications. However, the underlying cellular and molecular mechanisms sustaining the beneficial effects of bariatric surgery are not yet fully understood. Due to the numerous genetically-modified strains available, the mouse model is the most convenient animal model to explore the molecular mechanisms behind the pleiotropic beneficial effects of bariatric surgeries. Here, we detailed the optimized healthcare methods and surgical protocols in mice for the two most widely-used bariatric surgeries: the sleeve gastrectomy and the modified Roux-en-Y gastric bypass. Deciphering the molecular mechanisms underlying the therapeutic effects of bariatric surgeries offers the promise of identifying new therapeutics targets.
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes.
authors.forename: Audrey
authors.initials: A
authors.lastname: Ayer
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes.
authors.forename: Frederic
authors.initials: F
authors.lastname: Borel
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes.
authors.forename: Francois
authors.initials: F
authors.lastname: Moreau
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes.
authors.forename: Xavier
authors.initials: X
authors.lastname: Prieur
authors.affiliation: CHU Nantes, Institut des Maladies de l'Appareil Digestif, INSERM U913.
authors.forename: Michel
authors.initials: M
authors.lastname: Neunlist
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, CHU Nantes.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Cariou
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes; Service de Clinique Chirurgicale Digestive et Endocrinienne, CHU de Nantes.
authors.forename: Claire
authors.initials: C
authors.lastname: Blanchard
authors.affiliation: l'Institut du Thorax, INSERM, CNRS, UNIV Nantes; cedric.lemay@univ-nantes.fr.
authors.forename: Cedric
authors.initials: C
authors.lastname: Le May
mesh_terms: D000818:Animals; D050110:Bariatric Surgery; D015992:Body Mass Index; D005260:Female; D005743:Gastrectomy / Q000379:methods*; D015390:Gastric Bypass / Q000379:methods*; D010535:Laparoscopy / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009767:Obesity, Morbid; D016896:Treatment Outcome; D015431:Weight Loss