title: Salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA.
abstract: The yeast cell wall plays an important role in maintaining cell morphology, cell integrity and response to environmental stresses. Here, we report that salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA (ρ0). Upon salt treatment, the cell wall is thickened, broken and becomes more sensitive to the cell wall-perturbing agent sodium dodecyl sulfate (SDS). Also,SCW11mRNA levels are elevated in ρ0cells. Deletion ofSCW11significantly decreases the sensitivity of ρ0cells to SDS after salt treatment, while overexpression ofSCW11results in higher sensitivity. In addition, salt stress in ρ0cells induces high levels of reactive oxygen species (ROS), which further damages the cell wall, causing cells to become more sensitive towards the cell wall-perturbing agent.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qiuqiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
authors.forename: Liang-Chun
authors.initials: LC
authors.lastname: Liou
authors.affiliation: Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
authors.forename: Qun
authors.initials: Q
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Zhang
keywords: SCW11; Saccharomyces cerevisiae; cell wall damage; reactive oxygen species; salt stress