title: Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing.
abstract: Ultrasensitive biosensing technologies without gene amplification held great promise for direct detection of DNA. Herein we report a novel biosensing method, combining target recycling signal-amplification strategy and a homemade electrochemical device. Especially, the target recycling was achieved by a strand displacement process, no needing the help of any nucleases. In the presence of target DNA, the recycling system could be activated to generate a cascade of assembly steps with three hairpin DNA segments. Each recycling process were accompanied by a disassembly step that the last hairpin DNA segment displaces target DNA from the complex at the end of each circulation, freeing targets to activate the self-assembly of more trefoil DNA structures. This biosensing method could detect target DNA at aM level and can distinguish target DNA from interfering DNAs, demonstrating its high sensitivity and high selectivity. Importantly, the biosensing method could work well with serum samples.
authors.affiliation: NEST Lab, Department of Chemistry, Shanghai University, Shanghai 200444, China; Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, China.
authors.forename: Zhihan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, China.
authors.forename: Jianbang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: NEST Lab, Department of Chemistry, Shanghai University, Shanghai 200444, China. Electronic address: xujiaqiang@shu.edu.cn.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Inorganic Chemistry, University of Cologne, D-50939 Cologne, Germany.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Mathur
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 2019 Jialuo Road, Shanghai 201800, China. Electronic address: spsong@sinap.ac.cn.
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA detection; Multiplexing biosensing; Nuclease-free; Target recycling