title: Inhibitory effect ofSalvia plebeialeaf extract on ultraviolet-induced photoaging-associated ion channels and enzymes.
abstract: In traditional Korean/Asian medicine, Salvia plebeia R.Br. (S. plebeia) leaves are used to treat inflammatory diseases, including dermatitis, cough, asthma and toothache. Recently,S. plebeialeaves have been applied in skin care, as they promote skin lightening and elasticity. Therefore, the present study investigated the anti-aging effects ofS. plebeialeaf methanolic extract and its fractions (dichloromethane, ethylacetate and n-butanol). The results of a whole-cell patch clamp analysis indicated that the methanolic extract mediated ultraviolet (UV)-induced photoaging-associated ion channels, transient receptor potential vanilloid 1 (TRPV1) and calcium release-activated calcium channel protein 1 (ORAI1) channel activity in HEK293T cells overexpressing TRPV1 or ORAI1 and STIM1. Electrophysiological analysis revealed that the butanol fraction inhibited capsaicin-induced TRPV1 (84±8% at -60 mV/86±1% at 100 mV at 100 µg/ml) and ORAI1 (87±2% at -120 mV at 100 µg/ml) currents. Furthermore, the dichloromethane and hexane fractions inhibited tyrosinase activity by 32.4±0.69 and 22.6±0.96% at 330 µg/ml, respectively. Furthermore, the ethylacetate and butanol fractions inhibited elastase activity by 65.2±1.30 and 31.7±1.23% at 330 µg/ml, respectively. Tyrosinase and elastase, which are UV-induced photoaging-associated enzymes, regulate skin pigmentation and wrinkle formation, respectively. The results of the present study indicated thatS. plebeialeaves may be a novel treatment for UV-induced photoaging.
authors.affiliation: Department of Korean Medical Ophthalmology, Otolaryngology and Dermatology, Dongguk University College of Korean Medicine, Goyang 410-773, Republic of Korea.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Chang
authors.affiliation: Division of Bioscience, Dongguk University, Gyeongju 780-714, Republic of Korea.
authors.forename: Dong-Ung
authors.initials: DU
authors.lastname: Lee
authors.affiliation: Division of Bioscience, Dongguk University, Gyeongju 780-714, Republic of Korea.
authors.forename: Da Yeong
authors.initials: DY
authors.lastname: Nam
authors.affiliation: Department of Pediatrics, Dongguk University Ilsan Hospital, Goyang 410-773, Republic of Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Cho
authors.affiliation: Department of Pediatrics, Dongguk University Ilsan Hospital, Goyang 410-773, Republic of Korea.
authors.forename: Seungug
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Physiology, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea; Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang 410-773, Republic of Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Nam
authors.affiliation: Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang 410-773, Republic of Korea; Department of Internal Medicine, Graduate School of Medicine, Dongguk University, Goyang 410-773, Republic of Korea.
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Kim
keywords: Salvia plebeia; calcium channel; calcium release-activated calcium channel protein 1; elastase activity; transient receptor potential vanilloid 1; tyrosinase activity; ultraviolet-induced photoaging