title: Structure of acid deoxyribonuclease.
abstract: Deoxyribonuclease II (DNase II) is also known as acid deoxyribonuclease because it has optimal activity at the low pH environment of lysosomes where it is typically found in higher eukaryotes. Interestingly, DNase II has also been identified in a few genera of bacteria and is believed to have arisen via horizontal transfer. Here, we demonstrate that recombinant Burkholderia thailandensis DNase II is highly active at low pH in the absence of divalent metal ions, similar to eukaryotic DNase II. The crystal structure of B. thailandensis DNase II shows a dimeric quaternary structure which appears capable of binding double-stranded DNA. Each monomer of B. thailandensis DNase II exhibits a similar overall fold as phospholipase D (PLD), phosphatidylserine synthase (PSS) and tyrosyl-DNA phosphodiesterase (TDP), and conserved catalytic residues imply a similar mechanism. The structural and biochemical data presented here provide insights into the atomic structure and catalytic mechanism of DNase II.
authors.affiliation: Department of Biological Sciences, Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Armando
authors.initials: A
authors.lastname: Varela-Ramirez
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98110, USA.|Beryllium Discovery Corp., 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Abendroth
authors.affiliation: Department of Biological Sciences, Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Adrian A
authors.initials: AA
authors.lastname: Mejia
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98110, USA.|Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), 307 Westlake Ave N, Seattle, WA 98109, USA.
authors.forename: Isabelle Q
authors.initials: IQ
authors.lastname: Phan
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98110, USA.|Beryllium Discovery Corp., 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.
authors.forename: Donald D
authors.initials: DD
authors.lastname: Lorimer
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98110, USA.|Beryllium Discovery Corp., 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
authors.affiliation: Department of Biological Sciences, Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Renato J
authors.initials: RJ
authors.lastname: Aguilera
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D019117:Burkholderia / Q000201:enzymology*; D020134:Catalytic Domain; D003300:Copper / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D005057:Eukaryotic Cells / Q000201:enzymology; D019143:Evolution, Molecular; D022761:Gene Transfer, Horizontal; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D010802:Phylogeny; D011387:Prokaryotic Cells / Q000201:enzymology; D011487:Protein Conformation; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D003300:Copper; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II