title: Caveat emptor: for researchers, a rose will not smell sweet unless we know its composition.
abstract: In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan. The preparations were thus not suitable to study specific activities of decorin or biglycan. Decorin and biglycan are widely studied SLRPs that are considered to have highly multi-functional effects on cells. Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials. This Commentary discusses Brown et al.'s findings and general issues raised for researchers who work with commercially sourced purified proteoglycans.
authors.affiliation: School of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K. jo.adams@bristol.ac.uk.
authors.forename: Josephine C
authors.initials: JC
authors.lastname: Adams
mesh_terms: D053674:Aggrecans / Q000737:chemistry; D058578:Biglycan / Q000737:chemistry; D058575:Decorin / Q000737:chemistry; D000071235:Fibromodulin / Q000737:chemistry; D007930:Leucine / Q000737:chemistry*; D011509:Proteoglycans / Q000097:blood*; D023822:Tissue Engineering / Q000379:methods; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors
chemical_list: D053674:Aggrecans; D058578:Biglycan; D058575:Decorin; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D000071235:Fibromodulin; D007930:Leucine
keywords: SLRP; biglycan; decorin; neurite