title: Genotyping of commensal Neisseria spp strains by pulsed-field gel electrophoresis and 16S rRNA gene sequencing.
abstract: BACKGROUND
  We investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis.
  
  
  METHODS
  Identification to the genus was done using conventional methods and API NH (bio-Mérieux®). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using SpeI.
  
  
  RESULTS
  Fourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens, two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty-three different PFGE patterns were found among the tested strains.
  
  
  CONCLUSIONS
  We demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.
authors.affiliation: The National Bone Marrow Transplant Centre, Tunis, Tunisia.|Faculty of Medicine of Tunis, UR12ES02, Tunis, Tunisia.
authors.forename: Arij
authors.identifier: http://orcid.org/0000-0003-4433-5091
authors.initials: A
authors.lastname: Mechergui
authors.affiliation: The National Bone Marrow Transplant Centre, Tunis, Tunisia.|Faculty of Medicine of Tunis, UR12ES02, Tunis, Tunisia.
authors.forename: Wafa
authors.initials: W
authors.lastname: Achour
authors.affiliation: The National Bone Marrow Transplant Centre, Tunis, Tunisia.|Faculty of Medicine of Tunis, UR12ES02, Tunis, Tunisia.
authors.forename: Assia
authors.initials: A
authors.lastname: Ben Hassen
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D060005:Genotyping Techniques; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D009343:Neisseria / Q000145:classification / Q000235:genetics*; D016870:Neisseriaceae Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; commensal Neisseria; genotyping; pulsed-field gel electrophoresis