title: Clumping Factor B Promotes Adherence of Staphylococcus aureus to Corneocytes in Atopic Dermatitis.
abstract: Staphylococcus aureusskin infection is a frequent and recurrent problem in children with the common inflammatory skin disease atopic dermatitis (AD).S. aureuscolonizes the skin of the majority of children with AD and exacerbates the disease. The first step during colonization and infection is bacterial adhesion to the cornified envelope of corneocytes in the outer layer, the stratum corneum. Corneocytes from AD skin are structurally different from corneocytes from normal healthy skin. The objective of this study was to identify bacterial proteins that promote the adherence ofS. aureusto AD corneocytes.S. aureusstrains from clonal complexes 1 and 8 were more frequently isolated from infected AD skin than from the nasal cavity of healthy children. AD strains had increased ClfB ligand binding activity compared to normal nasal carriage strains. Adherence of singleS. aureusbacteria to corneocytes from AD patientsex vivowas studied using atomic force microscopy. Bacteria expressing ClfB recognized ligands distributed over the entire corneocyte surface. The ability of an isogenic ClfB-deficient mutant to adhere to AD corneocytes compared to that of its parent clonal complex 1 clinical strain was greatly reduced. ClfB from clonal complex 1 strains had a slightly higher binding affinity for its ligand than ClfB from strains from other clonal complexes. Our results provide new insights into the first step in the establishment ofS. aureuscolonization in AD patients. ClfB is a key adhesion molecule for the interaction ofS. aureuswith AD corneocytes and represents a target for intervention.
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Orla M
authors.initials: OM
authors.lastname: Fleury
authors.affiliation: Clinical Medicine, Trinity College Dublin, Dublin, Ireland.|National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.|Paediatric Dermatology, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.
authors.forename: Maeve A
authors.initials: MA
authors.lastname: McAleer
authors.affiliation: Institute of Life Sciences, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Feuillie
authors.affiliation: Institute of Life Sciences, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Formosa-Dague
authors.affiliation: Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Sansevere
authors.affiliation: Epidemiology and Molecular Biology Unit, Temple Street Children's University Hospital, Dublin, Ireland.
authors.forename: Désirée E
authors.initials: DE
authors.lastname: Bennett
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Aisling M
authors.initials: AM
authors.lastname: Towell
authors.affiliation: Dermatology and Genetic Medicine, University of Dundee, Dundee, United Kingdom.
authors.forename: W H Irwin
authors.initials: WHI
authors.lastname: McLean
authors.affiliation: Coronel Institute of Occupational Health, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: Sanja
authors.initials: S
authors.lastname: Kezic
authors.affiliation: Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
authors.forename: D Ashley
authors.initials: DA
authors.lastname: Robinson
authors.affiliation: Clinical Medicine, Trinity College Dublin, Dublin, Ireland.|National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.
authors.forename: Padraic G
authors.initials: PG
authors.lastname: Fallon
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Foster
authors.affiliation: Institute of Life Sciences, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.|Walloon Excellence in Life Sciences and Biotechnology (WELBIO), Wavre, Belgium.
authors.forename: Yves F
authors.initials: YF
authors.lastname: Dufrêne
authors.affiliation: Clinical Medicine, Trinity College Dublin, Dublin, Ireland.|National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.|Paediatric Dermatology, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.
authors.forename: Alan D
authors.initials: AD
authors.lastname: Irvine
authors.affiliation: Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland geoghegj@tcd.ie.
authors.forename: Joan A
authors.initials: JA
authors.lastname: Geoghegan
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology*; D005260:Female; D000091344:Filaggrin Proteins; D006801:Humans; D008297:Male; D009296:Nasal Cavity / Q000382:microbiology; D017384:Sequence Deletion; D012867:Skin / Q000166:cytology / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; C434058:clfB protein, Staphylococcus aureus
keywords: Staphylococcus aureus; atomic force microscopy; atopic dermatitis; corneocytes; filaggrin