title: Overexpression of miR-10b in colorectal cancer patients: Correlation with TWIST-1 and E-cadherin expression.
abstract: MicroRNAs are emergent players of epigenetics that function as oncogenes or tumor suppressors and that have been implicated in regulating diverse cellular pathways. MiR-10b is an oncogenic microRNA involved in tumor invasion and metastasis in various cancers. Our data have shown that miR-10b is overexpressed in colorectal cancer samples in comparison with non-tumorous adjacent mucosa (p = 0.0025) and that it is associated with severe features such as tumor size >5 cm (p = 0.023), distant metastasis (p = 0.0022), non-differentiated tumors (p = 0.016), and vascular invasion (p = 0.01). Regarding the regulation of its expression, positive correlation between the loss of miR-10b and aberrant DNA methylation (p = 0.02) as well as a loss of TWIST-1 messenger RNA (p = 0.018) have been observed. Furthermore, expression analysis of the downstream miR-10b targets has shown that there are associations between low HOXD10 messenger RNA and E-cadherin protein levels (p < 0.0001, p = 0.0008, respectively) and overexpression of miR-10b. Our data suggests that overexpression of miR-10b results from high levels of TWIST-1 and may induce a decrease of E-cadherin membranous protein levels, thus contributing to the acquisition of metastatic phenotypes in colorectal cancer.
authors.affiliation: 1 Laboratory of Eukaryotes Molecular Biotechnology, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Rania
authors.initials: R
authors.lastname: Abdelmaksoud-Dammak
authors.affiliation: 1 Laboratory of Eukaryotes Molecular Biotechnology, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Nour
authors.initials: N
authors.lastname: Chamtouri
authors.affiliation: 1 Laboratory of Eukaryotes Molecular Biotechnology, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Mouna
authors.initials: M
authors.lastname: Triki
authors.affiliation: 1 Laboratory of Eukaryotes Molecular Biotechnology, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Amena
authors.initials: A
authors.lastname: Saadallah-Kallel
authors.affiliation: 1 Laboratory of Eukaryotes Molecular Biotechnology, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Wajdi
authors.initials: W
authors.lastname: Ayadi
authors.affiliation: 2 Department of Anatomopathology, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Slim
authors.initials: S
authors.lastname: Charfi
authors.affiliation: 2 Department of Anatomopathology, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Khabir
authors.affiliation: 2 Department of Anatomopathology, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Lobna
authors.initials: L
authors.lastname: Ayadi
authors.affiliation: 2 Department of Anatomopathology, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Tahia
authors.initials: T
authors.lastname: Sallemi-Boudawara
authors.affiliation: 1 Laboratory of Eukaryotes Molecular Biotechnology, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Raja
authors.initials: R
authors.lastname: Mokdad-Gargouri
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D019073:HT29 Cells; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D051879:Twist-Related Protein 1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015820:Cadherins; D018398:Homeodomain Proteins; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D012333:RNA, Messenger; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1; C072064:HOXD10 protein, human
keywords: E-cadherin; HOXD10; MiR-10b; TWIST-1; methylation; regulation