title: Quantification of Cell Turnover in the Bovine Leukemia Virus Model.
abstract: In a perspective of a comparative virology approach, characterization of the bovine leukemia virus (BLV) model may be helpful to better understand infection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle. We also describe methods to quantify apoptosis ex vivo and cell turnover in vivo.
authors.affiliation: Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
authors.forename: Alix
authors.initials: A
authors.lastname: de Brogniez
authors.affiliation: Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Barez
authors.affiliation: Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Carpentier
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina.
authors.forename: Geronimo
authors.initials: G
authors.lastname: Gutierrez
authors.affiliation: Department of Pathology, National Veterinary Research Institute, Pulawy, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Reichert
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina.
authors.forename: Karina
authors.initials: K
authors.lastname: Trono
authors.affiliation: Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium. Luc.willems@ulg.ac.be.
authors.forename: Luc
authors.initials: L
authors.lastname: Willems
mesh_terms: D000818:Animals; D017209:Apoptosis; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000378:metabolism* / Q000473:pathology; D015368:Human T-lymphotropic virus 1; D006801:Humans; D001909:Leukemia Virus, Bovine / Q000378:metabolism*; D008954:Models, Biological; D012756:Sheep
keywords: Apoptosis; Bovine leukemia virus; CFDA-SE; Deltaretrovirus; HTLV-1; Proliferation