title: Kelussia odoratissimapotentiates cytotoxic effects of radiation in HeLa cancer cell line.
abstract: OBJECTIVE
  Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity ofKelussia odoratissima(K. odoratissima) extract associated with radiotherapy in cervical cancer cells (HeLa cell line).
  
  
  METHODS
  Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.
  
  
  RESULTS
  K. odoratissimadecreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,K. odoratissimainduced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved inK. odoratissima-induced toxicity. It was also shown thatK. odoratissimasensitizes cells to radiation-induced toxicity.
  
  
  CONCLUSIONS
  Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid.
authors.affiliation: Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Hosseini
authors.affiliation: Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Shima
authors.initials: S
authors.lastname: Saeidi Javadi
authors.affiliation: Department of Oncology, Omid Hospitsal, Mashhad, Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Fani-Pakdel
authors.affiliation: Pharmacological Research Center of Medicinal Plants, MashhadUniversity of Medical Sciences, Mashhad, Iran ; Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Mousavi
keywords: Apoptosis; Cytotoxicity; HeLa cell line; Kelussia odoratissima; Radiotherapy