title: Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture.
abstract: A three-dimensional (3D) tissue generated in vitro is a promising source to study developmental biology and regenerative medicine. In the last decade, Yoshiki Sasai's group have developed a 3D stem cell culture technique known as SFEBq and demonstrated that embryonic stem cells (ESCs) have an ability to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs.
authors.affiliation: In Vitro Histogenesis team, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan. atsushi-kuwahara@ds-pharma.co.jp.|Regenerative and Cellular Medicine Office, Sumitomo Dainippon Pharma Co., Ltd., Chuo Kobe, 650-0047, Japan. atsushi-kuwahara@ds-pharma.co.jp.|Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 3-1-98 Kasugade-naka, Konohana, Osaka, 554-8558, Japan. atsushi-kuwahara@ds-pharma.co.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuwahara
authors.affiliation: Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 3-1-98 Kasugade-naka, Konohana, Osaka, 554-8558, Japan.
authors.forename: Tokushige
authors.initials: T
authors.lastname: Nakano
authors.affiliation: In Vitro Histogenesis team, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan. eiraku@cdb.riken.jp.
authors.forename: Mototsugu
authors.initials: M
authors.lastname: Eiraku
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D012160:Retina / Q000166:cytology*
keywords: Ciliary margin; Human ESCs; Neural retina; Optic cup; RPE; Retina; SFEBq culture