title: Inducible protein stabilization system in Leishmania mexicana.
abstract: Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Podešvová
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Huan
authors.initials: H
authors.lastname: Huang
authors.affiliation: Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice (Budweis), Czech Republic; Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic. Electronic address: vyacheslav.yurchenko@osu.cz.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Yurchenko
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation; D007894:Leishmania mexicana / Q000235:genetics* / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014295:Trimethoprim / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase
keywords: Leishmania mexicana; Protein stabilization; Trimethoprim; ecDHFR