title: Cytokine gene polymorphisms in populations from Parana, Southern Brazil.
abstract: In this study, were genotyped 22 single nucleotide polymorphisms (SNPs) in 13 genes that encode the pro-inflammatory (IL-1α, IL-1β, IL-1R, IL-4Rα, IL-12, IFN-γ, TNF-α, and IL-2) and anti-inflammatory (IL-1RA, TGF-β, IL-4, IL-6 and IL-10) cytokines of 350 individuals by PCR-SSP (polymerase chain reaction - sequence specific primer). A total of 473 individuals were genotyped for IL17A and IL17F genes by PCR-RFLP (restriction fragment length polymorphism). The sample consisted of healthy and unrelated subjects from a mixed population from Parana state, in the South region of Brazil. The frequency analyses and genotype data are available in the Supplementary materials and are accessible at Allele Frequency Net Database (AFND).
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Pâmela Guimarães
authors.initials: PG
authors.lastname: Reis
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Josiane Bazzo
authors.initials: JB
authors.lastname: de Alencar
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Luciana Conci
authors.initials: LC
authors.lastname: Macedo
authors.affiliation: Department of Biotechnology, Genetics, and Cell Biology, Maringa State University, Parana, Brazil.
authors.forename: Eliane Papa
authors.initials: EP
authors.lastname: Ambrosio-Albuquerque
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Julimary Suematsu
authors.initials: JS
authors.lastname: de Aquino
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Joana Maira Valentini
authors.initials: JMV
authors.lastname: Zacarias
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil.
authors.forename: Patrícia Yumeko
authors.initials: PY
authors.lastname: Tsuneto
authors.affiliation: Laboratory of Immunogenetics, Department of Basic Health Sciences, Maringa State University, Parana, Brazil. Electronic address: ramoliterno@uem.br.
authors.forename: Ricardo Alberto
authors.initials: RA
authors.lastname: Moliterno
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil; Laboratory of Immunogenetics, Department of Basic Health Sciences, Maringa State University, Parana, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Sell
authors.affiliation: Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil; Laboratory of Immunogenetics, Department of Basic Health Sciences, Maringa State University, Parana, Brazil. Electronic address: jelvisentainer@gmail.com.
authors.forename: Jeane Eliete Laguila
authors.initials: JEL
authors.lastname: Visentainer
mesh_terms: D001938:Brazil; D016207:Cytokines / Q000235:genetics*; D030541:Databases, Genetic; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D020381:Interleukin-17 / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D016207:Cytokines; D020381:Interleukin-17
keywords: Allele frequency; Cytokine; Population; SNPs