title: Hyaluronate-Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer.
abstract: Colon cancer is one of the most common death-related cancers in the world. For treating colon cancer, it is crucial to detect and remove malignant lesions early. Here, we developed hyaluronate (HA)-peanut agglutinin (PNA) conjugates for the bioimaging of colon cancer. The HA-PNA conjugates were successfully synthesized by the coupling reaction between aldehyde-modified HA and the N-terminal amine group of PNA. For diagnostic imaging, rhodamine B (RhoB) was chemically conjugated onto PNA in HA-PNA conjugates. After intraluminal injection of HA-PNA-RhoB conjugates into tumor-bearing mice, small-sized colon cancers could be effectively visualized by ex vivo imaging with an in vivo imaging system (IVIS) and a two-photon microscope. With these results taken together, we could confirm the feasibility of HA-PNA-RhoB conjugates as a bioimaging agent for detecting colon cancers.
authors.affiliation: Department of Materials Science and Engineering and ‡Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-ro, Nam-gu, Pohang 37673, Korea.
authors.forename: Songeun
authors.initials: S
authors.lastname: Beack
authors.affiliation: Department of Materials Science and Engineering and ‡Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-ro, Nam-gu, Pohang 37673, Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Cho
authors.affiliation: Department of Materials Science and Engineering and ‡Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-ro, Nam-gu, Pohang 37673, Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering and ‡Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-ro, Nam-gu, Pohang 37673, Korea.
authors.forename: G-One
authors.initials: GO
authors.lastname: Ahn
authors.affiliation: Department of Materials Science and Engineering and ‡Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-ro, Nam-gu, Pohang 37673, Korea.
authors.forename: Sei Kwang
authors.identifier: 0000-0002-7718-6259
authors.initials: SK
authors.lastname: Hahn
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D016264:Dextran Sulfate / Q000633:toxicity; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods*; D019887:Peanut Agglutinin / Q000737:chemistry* / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D019887:Peanut Agglutinin; D006820:Hyaluronic Acid; D016264:Dextran Sulfate