title: Integration of Chemical, Genetic, and Bioinformatic Approaches Delineates Fungal Polyketide-Peptide Hybrid Biosynthesis.
abstract: To identify natural products and their associated biosynthetic genes from underutilized, difficult-to-manipulate microbes, chemical screening and bioinformatic analysis were employed to identify secondary metabolites and a potentially associated biosynthetic gene cluster. Subsequently, a heterologous expression system was used to confirm the identity of the gene cluster and the proposed biosynthetic mechanism. This approach successfully identified the curvupallide and spirostaphylotrichin biosynthetic pathways in endophytic fungus Curvularia pallescens and the short-chain pyranonigrin biosynthetic pathway in Aspergillus niger.
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yokoyama
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Hirayama
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kishimoto
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Tsunematsu
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Kenji
authors.identifier: 0000-0002-0463-4831
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D053898:Biosynthetic Pathways; D019295:Computational Biology; D005800:Genes, Fungal; D005810:Multigene Family; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D061065:Polyketides / Q000737:chemistry* / Q000302:isolation & purification; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification; D064210:Secondary Metabolism
chemical_list: D001688:Biological Products; D010455:Peptides; D061065:Polyketides; D011753:Pyrones