title: Single-cell RNA sequencing identifies distinct mouse medial ganglionic eminence cell types.
abstract: Many subtypes of cortical interneurons (CINs) are found in adult mouse cortices, but the mechanism generating their diversity remains elusive. We performed single-cell RNA sequencing on the mouse embryonic medial ganglionic eminence (MGE), the major birthplace for CINs, and on MGE-like cells differentiated from embryonic stem cells. Two distinct cell types were identified as proliferating neural progenitors and immature neurons, both of which comprised sub-populations. Although lineage development of MGE progenitors was reconstructed and immature neurons were characterized as GABAergic, cells that might correspond to precursors of different CINs were not identified. A few non-neuronal cell types were detected, including microglia. In vitro MGE-like cells resembled bona fide MGE cells but expressed lower levels of Foxg1 and Epha4. Together, our data provide detailed understanding of the embryonic MGE developmental program and suggest how CINs are specified.
authors.affiliation: Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Ying-Jiun J
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Bioinformatics and Computational Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Brad A
authors.initials: BA
authors.lastname: Friedman
authors.affiliation: Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Connie
authors.initials: C
authors.lastname: Ha
authors.affiliation: Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.|Department of Bioinformatics and Computational Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Steffen
authors.initials: S
authors.lastname: Durinck
authors.affiliation: Department of Bioinformatics and Computational Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Psychiatry, University of California San Francisco, CA 94158, USA.
authors.forename: John L
authors.initials: JL
authors.lastname: Rubenstein
authors.affiliation: Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Somasekar
authors.initials: S
authors.lastname: Seshagiri
authors.affiliation: Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
authors.forename: Zora
authors.initials: Z
authors.lastname: Modrusan
mesh_terms: D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D007395:Interneurons / Q000378:metabolism; D008473:Median Eminence / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis