title: LncRNA TUG1 is upregulated and promotes cell proliferation in osteosarcoma.
abstract: OBJECTIVE
  To examine the expression and function of long non-coding RNAtaurine up-regulated 1(TUG1) in human osteosarcoma cells.
  
  
  METHODS
  Real-time quantitive PCR was used to detect the transcription level ofTUG1in a series of osteosarcoma cell lines. Knockdown ofTUG1in U2OS cells was carried out by transient transfection of siRNAs. MTT assay was performed to access the cell growth rates. Afterwards, RNA and protein of these cells were extracted to analyze the transfection efficient as well as the expression of other molecules.
  
  
  RESULTS
  Compared to the normal cell line,TUG1exhibited a significant upregulation in osteosarcoma cells. Phenotyping analysis showed the growth-promotion activity ofTUG1, since knockdown ofTUG1resulted in declined proliferation. We also found that AKT phosphorylation was impaired afterTUG1was inhibited, suggesting that the AKT pathway was involved in the regulation ofTUG1in U2OS cells.
  
  
  CONCLUSIONS
  Our data provided evidence thatTUG1was upregulated and acted as a possible oncogene via positively regulating cell proliferation in osteosarcoma cells.
authors.affiliation: Should be: Tangshan Gongren Hospital, Tangshan Hebei Province 063000, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yun-Bo
authors.affiliation: Department of orthopaedics, Tangshan City workers Hospital, Tangshan Hebei Province 063000 PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Xiao-Po
authors.affiliation: Department of geriatrics, Tangshan City workers Hospital, Tangshan Hebei Province 063000, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao-Li
authors.affiliation: Department of orthopaedics, Tangshan City workers Hospital, Tangshan Hebei Province 063000 PR China.
authors.forename: Cao
authors.initials: C
authors.lastname: Guo-Long
authors.affiliation: Department of neurology, Tangshan City workers Hospital, Tangshan Hebei Province 063000, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Pei
authors.affiliation: Center for medical experimental research, North China University of Science and Technology, Tangshan Hebei Province 063000, PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Fa-Ming
keywords: AKT; TUG1; osteosarcoma; proliferation