title: Development of novel monoclonal antibodies directed against catechins for investigation of antibacterial mechanism of catechins.
abstract: Catechins are major polyphenolic compounds of green tea. To investigate mechanism for antibacterial action of catechins, 11 monoclonal antibodies (MAbs) were raised against a 3-succinyl-epicatechin (EC)-keyhole limpet hemocyanin (KLH) conjugate. Amino acid sequences of variable regions determined for MAbs b-1058, b-1565, and b-2106 confirmed their innovative character. MAb b-1058 strongly interacted with its target substances in the following order of magnitude: theaflavin-3,3'-di-O-gallate (TFDG)>theaflavin-3-O-gallate (TF3G)≥theaflavin-3'-O-gallate (TF3'G)>gallocatechin gallate (GCg)>penta-O-galloyl-β-d-glucose (PGG)>epigallocatechin gallate (EGCg), as determined using surface plasmon resonance (SPR) on MAb-immobilized sensor chips. The affinity profiles of MAbs b-1058 and b-2106 to the various polyphenols tested suggested that flavan skeletons with both carbonyl oxygen and hydroxyl groups are important for this interaction to take place. S. aureus cells treated with EGCg showed green fluorescence around the cells after incubation with FITC-labeled MAb b-1058. The fluorescence intensity increased with increasing concentrations of EGCg. These MAbs are effective to investigate antibacterial mechanism of catechins and theaflavins.
authors.affiliation: Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: tmiyamot@agr.kyushu-u.ac.jp.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: College of Food Science and Engineering, Jilin University, No. 5988 Renmin Street, Nanguan District, Changchun 130022, PR China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Ueyama
authors.affiliation: Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Kitichalermkiat
authors.initials: K
authors.lastname: Apisada
authors.affiliation: R&D Core Technology Safety Science Research, Kao Corporation, Akabane 2606, Haga-Gun, Ichikai-Machi, Tochigi 321-3497, Japan.
authors.forename: Motokazu
authors.initials: M
authors.lastname: Nakayama
authors.affiliation: Product Quality Management, Chemicals Regulation, Global, Kao Corporation, 2-1-3, Bunka, Sumida-ku, Tokyo 131-8501, Japan.
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Bioscience Research, Kao Corporation, Akabane 2606, Haga-Gun, Ichikai-Machi, Tochigi 321-3497, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Ozawa
authors.affiliation: R&D Core Technology Safety Science Research, Kao Corporation, Akabane 2606, Haga-Gun, Ichikai-Machi, Tochigi 321-3497, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Mitani
authors.affiliation: R&D Core Technology Safety Science Research, Kao Corporation, Akabane 2606, Haga-Gun, Ichikai-Machi, Tochigi 321-3497, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Shigemune
authors.affiliation: R&D Core Technology Safety Science Research, Kao Corporation, Akabane 2606, Haga-Gun, Ichikai-Machi, Tochigi 321-3497, Japan.
authors.forename: Kanami
authors.initials: K
authors.lastname: Shimatani
authors.affiliation: R&D Core Technology Safety Science Research, Kao Corporation, Akabane 2606, Haga-Gun, Ichikai-Machi, Tochigi 321-3497, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yui
authors.affiliation: Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Honjoh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology* / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001483:Base Sequence; D002392:Catechin / Q000276:immunology* / Q000494:pharmacology*; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D006433:Hemocyanins / Q000276:immunology; D008807:Mice, Inbred BALB C; D059808:Polyphenols / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D059808:Polyphenols; D002392:Catechin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
keywords: EGCg; GCg; Monoclonal antibody; Myricetin; Theaflavins