title: An Azole-Tolerant Endosomal Trafficking Mutant of Candida albicans Is Susceptible to Azole Treatment in a Mouse Model of Vaginal Candidiasis.
abstract: We recently reported that aCandida albicansendosomal trafficking mutant continues to grow after treatment with the azole antifungals. Herein, we report that thevps21Δ/Δ mutant does not have a survival advantage over wild-type isolates after fluconazole treatment in a mouse model of vaginal candidiasis. Furthermore, loss ofVPS21does not synergize with established mechanisms of azole resistance, such as overexpression of efflux pumps or of Erg11p, the target enzyme of the azoles. In summary, although loss ofVPS21function enhancesC. albicanssurvival after azole treatmentin vitro, it does not seem to affect azole susceptibilityin vivo.
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA.|Department of Oral and Craniofacial Biology, School of Dentistry, LSU Health Sciences Center, New Orleans, Louisiana, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Peters
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA.
authors.forename: Arturo
authors.initials: A
authors.lastname: Luna-Tapia
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA.
authors.forename: Hélène
authors.initials: H
authors.lastname: Tournu
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Rybak
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA.
authors.forename: P David
authors.initials: PD
authors.lastname: Rogers
authors.affiliation: Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA gpalmer5@uthsc.edu.
authors.forename: Glen E
authors.initials: GE
authors.lastname: Palmer
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D002181:Candidiasis, Vulvovaginal / Q000188:drug therapy* / Q000382:microbiology; D004195:Disease Models, Animal; D025141:Drug Resistance, Fungal / Q000235:genetics; D005260:Female; D015725:Fluconazole / Q000627:therapeutic use*; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D020691:rab GTP-Binding Proteins / Q000235:genetics
chemical_list: D000935:Antifungal Agents; D026901:Membrane Transport Proteins; D015725:Fluconazole; D020691:rab GTP-Binding Proteins
keywords: Candida albicans; Vps21p; azoles; resistance; tolerance; vaginal candidiasis