title: Influence of mixed electrolytes on the adsorption of lysozyme, PEG, and PEGylated lysozyme on a hydrophobic interaction chromatography resin.
abstract: In a recent work (Werner A and Hasse H in J Chromatogr A 2013;1315:135) the influence of mixed electrolytes on the adsorption of the macromolecules lysozyme, PEG and di-PEGylated lysozyme on a hydrophobic resin has been studied, but only at one overall ionic strength (3000 mM). The present work, therefore, extends these studies to other ionic strengths (2400 and 2700 mM), and explores the application of a model to predict the entire data set. The adsorbent is Toyopearl PPG-600M. The solvent is a 25 mM aqueous sodium phosphate buffer at pH 7.0. The studied salts are sodium chloride, sodium sulfate, ammonium chloride and ammonium sulfate. Pure salts as well as binary and ternary mixtures of these salts with varying ratios of the amounts of the salts are studied at 25 °C. The loading of the adsorbent increases with increasing salt concentration for all macromolecules. Synergetic effects of the mixed electrolytes are observed. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1104-1115, 2017.
authors.affiliation: Laboratory of Engineering Thermodynamics, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Eva
authors.identifier: http://orcid.org/0000-0002-0296-7253
authors.initials: E
authors.lastname: Hackemann
authors.affiliation: Laboratory of Engineering Thermodynamics, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Albert
authors.initials: A
authors.lastname: Werner
authors.affiliation: Laboratory of Engineering Thermodynamics, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Hasse
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000737:chemistry*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004573:Electrolytes; D012117:Resins, Synthetic; D011092:Polyethylene Glycols; C421864:hen egg lysozyme; D009113:Muramidase
keywords: PEG; hydrophobic interaction chromatography (HIC); ionic strength; lysozyme; mixed electrolytes; modeling