title: Fabrication, Characterization, and Hemocompatibility Investigation of Polysulfone Grafted With Polyethylene Glycol and Heparin Used in Membrane Oxygenators.
abstract: Polysulfone (PSF) was grafted chemically with polyethylene glycol (PEG) of different molecular weights (400, 2000, 10 000, and 20 000) and heparin to prepare PSF-PEG-Hep membranes (labeled as PSF-PEG400-Hep, PSF-PEG2000-Hep, PSF-PEG10 000-Hep, and PSF-PEG20 000-Hep). These membranes were synthesized via the following steps: (i) PSF chloromethylation; (ii) PEGylation; and (iii) heparin immobilization process. Water contact angle, critical water permeation pressure, ATR-FTIR,1H NMR, UV-visible spectrophotometry, and X-ray photoelectron spectroscopy were conducted to prove grafting success and to fix the optimal reaction parameters during chemical modification processes. To further evaluate the application potential of the PSF-PEG-Hep membrane in a membrane oxygenator, we conducted pure CO2and O2gas permeation tests and measured the gas exchange rates of CO2and O2through a membrane oxygenator using fresh porcine blood. Meanwhile, the hemocompatibility of the membrane was analyzed and compared by protein adsorption, platelet adhesion, and blood coagulation tests. Results of pure gas permeation experiments indicated that the excellent gas transmission properties through PSF membrane were preserved after modification. Blood oxygenation experiments through the PSF-PEG10 000-Hep membrane showed that when the flow rate of porcine blood was 1.5 L/min, CO2and O2exchange rates reached 102 and 110 mL/min, respectively, which could basically satisfy the gas exchange potential of commercial membrane oxygenators. Besides, the PSF-PEG-Hep membrane has demonstrated more prominent blood compatibility than PSF.
authors.affiliation: Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Nanjing University.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Nanjing University.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Nanjing University.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Nanjing University.
authors.forename: Quan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine.
authors.forename: Wenling
authors.initials: W
authors.lastname: Fan
authors.affiliation: Medical School, Nanjing University, Nanjing, P. R. China.
authors.forename: Wenkui
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Nanjing University.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Nanjing University.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D001780:Blood Coagulation Tests; D006493:Heparin / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D010107:Oxygenators, Membrane; D010973:Platelet Adhesiveness; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D011092:Polyethylene Glycols; D006493:Heparin
keywords: Chemical grafting; Gas exchange rate; Hemocompatibility; Heparin membranes; Membrane oxygenator; Polyethylene glycol; Polysulfone