title: Functional interaction of fibroblast growth factor 8b and androgen in prostate cancer cell proliferation.
abstract: Fibroblast growth factor 8b and androgen play important roles in cell proliferation of prostate cancer. We investigated the effects of fibroblast growth factor 8b and androgen on the proliferation of prostate cell lines and the corresponding intracellular mechanisms. It is found that dihydrotestosterone and fibroblast growth factor 8b stimulated Lncap cell mitosis in a concentration-responsive manner, with 30 ng/mL as the most suitable concentration, respectively. Dihydrotestosterone treatment alone did not enhance the expression and phosphorylation level of fibroblast growth factor receptor but significantly enhanced the level of fibroblast growth factor receptor phosphorylation elicited by fibroblast growth factor 8b. Phosphorylations of extracellular signal-regulated kinase, p38, and c-Jun NH2-terminal kinase were stimulated by dihydrotestosterone or fibroblast growth factor 8b. Among these major downstream pathways for mitogen-activated protein kinase, c-Jun NH2-terminal kinase signaling was most significantly enhanced. Protein kinase C phosphorylation was higher than AKT by the combined stimulation of dihydrotestosterone and fibroblast growth factor 8b. The phosphorylation of CDC2 was significantly induced by dihydrotestosterone and fibroblast growth factor 8b synergetically, and Smad underwent the same induction as CDC2. So the promoting effect of fibroblast growth factor 8b on cell cycle might contribute to the G2/M transition. This study indicated that the functional interaction between fibroblast growth factor 8b and androgen was essential for the prostate cancer cell proliferation.
authors.affiliation: 1 Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.|2 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Xiao
authors.affiliation: 1 Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.|2 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.|3 Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Gui-Ming
authors.initials: GM
authors.lastname: Zhang
authors.affiliation: 1 Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.|2 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
authors.forename: Ding-Wei
authors.initials: DW
authors.lastname: Ye
mesh_terms: D016203:CDC2 Protein Kinase; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D013196:Dihydrotestosterone / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051524:Fibroblast Growth Factor 8 / Q000378:metabolism / Q000494:pharmacology*; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000473:pathology*; D051785:Smad Proteins / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C496456:FGF8 protein, human; D051785:Smad Proteins; D013196:Dihydrotestosterone; D051524:Fibroblast Growth Factor 8; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Fibroblast growth factor; androgen; proliferation; prostate cancer