title: MiR-613 suppresses retinoblastoma cell proliferation, invasion, and tumor formation by targeting E2F5.
abstract: Retinoblastoma is a common intraocular malignancy that occurs during childhood. MicroRNAs play critical roles in the regulation of retinoblastoma initiation and progression, and aberrant expression of miR-613 had been reported in various types of cancer. However, the role and mechanism of its function in retinoblastoma are still unclear. In this study, we found that miR-613 was downregulated in retinoblastoma tissues and cell lines. Overexpression of miR-613 suppressed retinoblastoma cell proliferation, migration, and invasion and induced cell cycle arrest in vitro. Additionally, overexpressed miR-613 also inhibited tumor formation of retinoblastoma cells in vivo. We further identified E2F5 as a direct target of miR-613. Reintroduction of E2F5 without 3'-untranslated region reversed the inhibitory effects of miR-613 on cell proliferation and invasion. Our data collectively indicate that miR-613 functions as a tumor suppressor in retinoblastoma through downregulating E2F5, supporting the targeting of the novel miR-613/E2F5 axis as a potentially effective therapeutic approach for retinoblastoma.
authors.affiliation: 1 Department of Ophthalmology, Medical School of Nanjing University, Jinling Hospital, Nanjing, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 Department of Ophthalmology, Medical School of Nanjing University, Jinling Hospital, Nanjing, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhu
authors.affiliation: 2 Department of Ophthalmology, Jinling Hospital, Nanjing, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: 2 Department of Ophthalmology, Jinling Hospital, Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 1 Department of Ophthalmology, Medical School of Nanjing University, Jinling Hospital, Nanjing, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 1 Department of Ophthalmology, Medical School of Nanjing University, Jinling Hospital, Nanjing, China.
authors.forename: Borui
authors.initials: B
authors.lastname: Yao
authors.affiliation: 1 Department of Ophthalmology, Medical School of Nanjing University, Jinling Hospital, Nanjing, China.
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Huang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D050700:E2F5 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology
chemical_list: D050700:E2F5 Transcription Factor; C092558:E2F5 protein, human; C561374:MIRN613 microRNA, human; D035683:MicroRNAs
keywords: E2F5; Retinoblastoma; miR-613