title: Honing Cell and Tissue Culture Conditions for Bone and Cartilage Tissue Engineering.
abstract: An avenue of tremendous interest and need in health care encompasses the regeneration of bone and cartilage. Over the years, numerous tissue engineering strategies have contributed substantial progress toward the realization of clinically relevant therapies. Cell and tissue culture protocols, however, show many variations that make experimental results among different publications challenging to compare. This collection surveys prevalent cell sources, soluble factors, culture medium formulations, environmental factors, and genetic modification approaches in the literature. The intent of consolidating this information is to provide a starting resource for scientists considering how to optimize the parameters for cell differentiation and tissue culture procedures within the context of bone and cartilage tissue engineering.
authors.affiliation: Department of Bioengineering, Rice University, Houston, Texas 77251.
authors.forename: Johnny
authors.initials: J
authors.lastname: Lam
authors.affiliation: Department of Bioengineering, Rice University, Houston, Texas 77251.
authors.forename: Esther J
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, Rice University, Houston, Texas 77251.
authors.forename: Elisa C
authors.initials: EC
authors.lastname: Clark
authors.affiliation: Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77251.
authors.forename: Antonios G
authors.initials: AG
authors.lastname: Mikos
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology*; D002356:Cartilage / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D006801:Humans; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*