title: SF3B4 is decreased in pancreatic cancer and inhibits the growth and migration of cancer cells.
abstract: Splicing factor 3b subunit 4, a critical component of pre-message RNA splicing complex, has been reported to play an important part in the tumorigenesis. However, the expression pattern and biological role of splicing factor 3b subunit 4 in pancreatic cancer have never been investigated. In this study, we found that both the messenger RNA ( p < 0.001) and protein level of splicing factor 3b subunit 4 were decreased significantly in pancreatic cancer specimens compared with their adjacent normal tissues. Overexpression of splicing factor 3b subunit 4 in pancreatic cancer cells inhibited cell growth and motility in vitro, while suppressing splicing factor 3b subunit 4 expression promoted the proliferation and migration of pancreatic cancer cells. In addition, splicing factor 3b subunit 4 was found to inhibit the activity of signal transducer and activator of transcription 3 signaling via downregulating the phosphorylation of signal transducer and activator of transcription 3 on a tyrosine residue at position 705. Taken together, these findings demonstrated that splicing factor 3b subunit 4 acted as a suppressive role in pancreatic cancer and indicated that restoring the function of splicing factor 3b subunit 4 might be a strategy for cancer therapy.
authors.affiliation: 1 Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhou
authors.affiliation: 2 Laboratory of Molecular Oncology, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: 3 East China University of Science and Technology, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: 1 Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Rong
authors.affiliation: 2 Laboratory of Molecular Oncology, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yuezhen
authors.initials: Y
authors.lastname: Deng
authors.affiliation: 2 Laboratory of Molecular Oncology, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: 1 Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Zhu
authors.affiliation: 1 Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Tiantao
authors.initials: T
authors.lastname: Kuang
authors.affiliation: 1 Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Lou
authors.affiliation: 2 Laboratory of Molecular Oncology, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Shanghai, China.|3 East China University of Science and Technology, Shanghai, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Xie
authors.affiliation: 1 Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Dansong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012326:RNA Splicing / Q000235:genetics; D000072260:RNA Splicing Factors / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: D000072260:RNA Splicing Factors; C486347:SF3B4 protein, human; D050796:STAT3 Transcription Factor
keywords: Splicing factor 3b subunit 4; cell migration; cell proliferation; pancreatic cancer; signal transducer and activator of transcription 3 signaling