title: Highly sensitive electrochemical immunoassay integrated with polymeric nanocomposites and enhanced SiO2@Au core-shell nanobioprobes for SirT1 determination.
abstract: An ultra-highly sensitive electrochemical immunosensor for SirT1 (a key protein in age-related diseases) evaluation has been designed, employing polymeric nanocomposites as sensing platform and core-shell SiO2@Au to immobilize HRP-Ab2as nanobioprobes. The approach includes chemical synthesis of PAMAM-Au-MWCNT nanocomposites (PNCs) with abundant PAMAM-Au nanoparticles immobilized on the MWCNT matrix, and biochemically synthesis of nanobioprobes with highly dispersed SiO2@Au tracing tags for successive efficient load functionalized enzyme-antibodies (HRP-Ab2). The PNCs nanocomposites could improve the efficiency of immune response via abundant capture antibodies (Ab1#1) on the electrode surface, and accelerate electron transfer through MWCNT and Au nanoparticles. Besides, the SiO2@Au was employed as tracing tags to label numerous HRP-Ab2to further enhance signal readout during HRP-thionine-H2O2system. Under optimal conditions, the signal intensity was linearly related to the concentration of SirT1 in the range of 20 pg mL-1to 500 ng ml-1, and the limit of detection was 12.5 pg mL-1. It is noteworthy that the proposed immunoassay protocol has been successfully applied to evaluate SirT1 expression in cells by different treatment with high sensitivity and accuracy.
authors.affiliation: College of Science, University of Shanghai for Science and Technology, Shanghai 200093, PR China; School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: anyarui@usst.edu.cn.
authors.forename: Yarui
authors.initials: Y
authors.lastname: An
authors.affiliation: College of Science, University of Shanghai for Science and Technology, Shanghai 200093, PR China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Zibo City Public Security, Zibo, 255000, PR China.
authors.forename: Wenji
authors.initials: W
authors.lastname: Bi
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Lu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Chongchong
authors.initials: C
authors.lastname: Feng
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: zaxu@chem.ecnu.edu.cn.
authors.forename: Zhiai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000072283:A549 Cells; D055910:Antibodies, Immobilized; D050091:Dendrimers; D055664:Electrochemical Techniques; D006046:Gold; D006801:Humans; D006861:Hydrogen Peroxide; D007118:Immunoassay; D057230:Limit of Detection; D053768:Metal Nanoparticles; D053761:Nanocomposites; D009757:Nylons; D012822:Silicon Dioxide; D056564:Sirtuin 1 / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; D006046:Gold; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Core-shell nanobioprobes; Electrochemistry; Immunoassay; Polymeric nanocomposites; SirT1