title: Effects of Catechins and Their Related Compounds on Cellular Accumulation and Efflux Transport of Mitoxantrone in Caco-2 Cell Monolayers.
abstract: The ability of catechins and their related compounds to inhibit breast cancer resistance protein (BCRP) function in Caco-2 cell monolayers was investigated with mitoxantrone as a BCRP substrate. The gallate or pyrogallol moiety on the catechin structure seemed to promote increased cellular accumulation and inhibit efflux transport of mitoxantrone. The ability of gallate catechins such as (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) to increase cellular accumulation and inhibit efflux transport of mitoxantrone was greater than that of nongallate catechins. Gallic acid octyl ester (GAO) also increased intracellular mitoxantrone accumulation. Experiments using GAO derivatives indicated that the gallate moiety required the presence of a long carbon chain for BCRP inhibition. Cellular accumulation and reduced efflux transport of mitoxantrone were greater with epigallocatechin 3-(3″-O-butyl) gallate than with EGCG. EGCG inhibition of BCRP seemed to be restricted by hydrophobicity. The co-administration of catechins, particularly EGCG and related compounds, with greater hydrophobicity may increase the therapeutic activities of BCRP substrates such as mitoxantrone.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama Univ., Sanzou 1,Gakuen-cho, Fukuyama, Hiroshima, Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Sugihara
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama Univ., Sanzou 1,Gakuen-cho, Fukuyama, Hiroshima, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Kuroda
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama Univ., Sanzou 1,Gakuen-cho, Fukuyama, Hiroshima, Japan.
authors.forename: Fumiya
authors.initials: F
authors.lastname: Watanabe
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama Univ., Sanzou 1,Gakuen-cho, Fukuyama, Hiroshima, Japan.
authors.forename: Tominari
authors.initials: T
authors.lastname: Choshi
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama Univ., Sanzou 1,Gakuen-cho, Fukuyama, Hiroshima, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kamishikiryo
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama Univ., Sanzou 1,Gakuen-cho, Fukuyama, Hiroshima, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Seo
mesh_terms: D000700:Analgesics / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006801:Humans; D008942:Mitoxantrone / Q000378:metabolism*; D013662:Tea / Q000737:chemistry
chemical_list: D000700:Analgesics; D013662:Tea; D002392:Catechin; D008942:Mitoxantrone
keywords: breast cancer resistance protein; catechin; gallic acid octyl ester; hydrophobicity; mitoxantrone