title: MiR-21/PTEN Axis Promotes Skin Wound Healing by Dendritic Cells Enhancement.
abstract: A number of miRNAs associated with wound repair have been identified and characterized, but the mechanism has not been fully clarified. MiR-21 is one of wound-related lncRNAs, and the study aimed to explore the functional involvement of miR-21 and its concrete mechanism in wound healing. In this study, the rat model of skin wounds was established. The expression of miR-21, PTEN and related molecules of wound tissues or cells was determined by quantitative real-time PCR and Western blot, respectively. The regulatory role of miR-21 on PTEN was examined by luciferase reporter gene assay. Flow cytometry assay was applied to measure cell number changes. MiR-21 was upregulated at 6, 24, 48, 72 h after model establishment, and the increase reached a maximum at 24 h in wound tissues. MMP-9 expression presented the same tread as miR-21 and was significantly enhanced within 6 h of wound formation, and then remained to be increased to the maximum at 24 h. The increase of miR-21 was accompanied by the increase of cell total number and DCs ratio in wound fluids. MiR-21 overexpression significantly improved the healing of skin wounds and increased the ratio of DCs in rats. The results of using FL confirmed that miR-21 overexpression obviously promoted DCs differentiation. Additionally, miR-21 could activate AKT/PI3K signaling pathway via inhibition of PTEN. MiR-21 contributes to wound healing via inhibition of PTEN that activated AKT/PI3K signaling pathway to increase DCs. J. Cell. Biochem. 118: 3511-3519, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Burn and Reconstruction, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Han
authors.affiliation: Department of Burn and Reconstruction, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Yile
authors.identifier: 0000-0002-0961-5613
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Burn and Reconstruction, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Aizhou
authors.initials: A
authors.lastname: Wei
authors.affiliation: Department of Burn and Reconstruction, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Burn and Reconstruction, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D035683:MicroRNAs / Q000096:biosynthesis*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D014945:Wound Healing
chemical_list: D035683:MicroRNAs; C546082:mirn21 microRNA, rat; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat
keywords: AKT/PI3K; DENDRITIC CELLS (DCS); MiR-21; PTEN; WOUND HEALING