title: Molecular characterisation of Salmonella strains isolated from outbreaks and sporadic cases of diarrhoea occurred in Paraná State, South of Brazil.
abstract: A total of 46 strains of Salmonella isolated from patients with sporadic diarrhoea or involved in foodborne outbreaks were analysed by PCR for genus identification and serotyping. Subtyping was performed using pulsed-field gel electrophoresis (PFGE) and multiple amplification of phage locus typing (MAPLT) for seven variable loci. Bacteria were identified as belonging to serotype Enteritidis (33 strains; 71·7%) or Typhimurium (13 strains; 28·3%). A high similarity coefficient (94·6%) was observed in the Salmonella Enteritidis group for which were found three related PFGE profiles and only one MAPLT; strains representing profile PA/P1/MI were prevalent (27; 81·8%). Two Salmonella Typhimurium isolates were untypeable by PFGE. The remaining 11 strains had eight PFGE and three MAPLT profiles. The discriminatory power of MAPLT was lower than that of PFGE. Salmonella Enteritidis of clonal nature is predominant in Paraná State, with the most prevalent profile PA/P1/M1 associated with sporadic diarrhoea and with seven of nine reported outbreaks. In conclusion, PFGE shows higher discriminatory power among Salmonella strains.
authors.affiliation: Departamento de Análises Clínicas,Universidade Federal do Paraná,Curitiba-PR,Brazil.
authors.forename: F E A
authors.initials: FEA
authors.lastname: Assis
authors.affiliation: Departamento de Análises Clínicas,Universidade Federal do Paraná,Curitiba-PR,Brazil.
authors.forename: C B
authors.initials: CB
authors.lastname: Dallagassa
authors.affiliation: Laboratório Central do Estado do Paraná,Curitiba-PR,Brazil.
authors.forename: S M S S
authors.initials: SMSS
authors.lastname: Farah
authors.affiliation: Departamento de Bioquímica e Biologia Molecular,Universidade Federal do Paraná,Curitiba-PR,Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Departamento de Bioquímica e Biologia Molecular,Universidade Federal do Paraná,Curitiba-PR,Brazil.
authors.forename: F O
authors.initials: FO
authors.lastname: Pedrosa
authors.affiliation: Departamento de Bioquímica e Biologia Molecular,Universidade Federal do Paraná,Curitiba-PR,Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Chubatsu
authors.affiliation: Departamento de Análises Clínicas,Universidade Federal do Paraná,Curitiba-PR,Brazil.
authors.forename: C M T
authors.identifier: 0000-0002-0478-6544
authors.initials: CMT
authors.lastname: Fadel-Picheth
mesh_terms: D001938:Brazil / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004196:Disease Outbreaks; D006801:Humans; D010802:Phylogeny; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial
keywords: 
                 Salmonella
              ; MAPLT; PFGE; molecular epidemiology