title: CD8+ T-cell mediated anti-malaria protection induced by malaria vaccines; assessment of hepatic CD8+ T cells by SCBC assay.
abstract: Malaria is a severe infectious disease with relatively high mortality, thus having been a scourge of humanity. There are a few candidate malaria vaccines that have shown a protective efficacy in humans against malaria. One of the candidate human malaria vaccines, which is based on human malaria sporozoites and called PfSPZ Vaccine, has been shown to protect a significant proportion of vaccine recipients from getting malaria. PfSPZ Vaccine elicits a potent response of hepatic CD8+ T cells that are specific for malaria antigens in non-human primates. To further characterize hepatic CD8+ T cells induced by the sporozoite-based malaria vaccine in a mouse model, we have used a cutting-edge Single-cell Barcode (SCBC) assay, a recently emerged approach/method for investigating the nature of T-cells responses during infection or cancer. Using the SCBC technology, we have identified a population of hepatic CD8+ T cells that are polyfunctional at a single cell level only in a group of vaccinated mice upon malaria challenge. The cytokines/chemokines secreted by these polyfunctional CD8+ T-cell subsets include MIP-1α, RANTES, IFN-γ, and/or IL-17A, which have shown to be associated with protective T-cell responses against certain pathogens. Therefore, a successful induction of such polyfunctional hepatic CD8+ T cells may be a key to the development of effective human malaria vaccine. In addition, the SCBC technology could provide a new level of diagnostic that will allow for a more accurate determination of vaccine efficacy.
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Alaina
authors.initials: A
authors.lastname: Kaiser
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Colin
authors.initials: C
authors.lastname: Ng
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Karcher
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Tim
authors.initials: T
authors.lastname: McConnell
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Paczkowski
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Fernandez
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Mackay
authors.affiliation: a IsoPlexis , Branford , CT , USA.|b Aaron Diamond AIDS Research Center , Affiliate of the Rockefeller University , New York , NY , USA.
authors.forename: Moriya
authors.identifier: 0000-0001-8587-277X
authors.initials: M
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003584:Cytological Techniques / Q000379:methods; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D008099:Liver / Q000276:immunology*; D008288:Malaria / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice
chemical_list: D017780:Malaria Vaccines