title: Bacterial kinesin light chain (Bklc) links the Btub cytoskeleton to membranes.
abstract: Bacterial kinesin light chain is a TPR domain-containing protein encoded by the bklc gene, which co-localizes with the bacterial tubulin (btub) genes in a conserved operon in Prosthecobacter. Btub heterodimers show high structural homology with eukaryotic tubulin and assemble into head-to-tail protofilaments. Intriguingly, Bklc is homologous to the light chain of the microtubule motor kinesin and could thus represent an additional eukaryotic-like cytoskeletal element in bacteria. Using biochemical characterization as well as cryo-electron tomography we show here that Bklc interacts specifically with Btub protofilaments, as well as lipid vesicles and could thus play a role in anchoring the Btub filaments to the membrane protrusions in Prosthecobacter where they specifically localize in vivo. This work sheds new light into possible ways in which the microtubule cytoskeleton may have evolved linking precursors of microtubules to the membrane via the kinesin moiety that in today's eukaryotic cytoskeleton links vesicle-packaged cargo to microtubules.
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR3348, F-91405 Orsay, France.|Université Paris Sud, Université Paris-Saclay, CNRS UMR3348, F-91405 Orsay, France.
authors.forename: Lurlène
authors.initials: L
authors.lastname: Akendengue
authors.affiliation: INSERM, U1196, Université Paris Sud, Université Paris-Saclay, F-91405 Orsay, France.|Institut Curie, PSL Research University, CNRS UMR9187, F-91405 Orsay, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Trépout
authors.affiliation: Institut Pasteur Montevideo, Unidad de Bioinformática, Mataojo 2020, 11400 Montevideo, Uruguay.
authors.forename: Martín
authors.initials: M
authors.lastname: Graña
authors.affiliation: Institut Pasteur, Unité de Biochimie des Interactions Macromoléculaires, CNRS UMR3528, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Voegele
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR3348, F-91405 Orsay, France.|Université Paris Sud, Université Paris-Saclay, CNRS UMR3348, F-91405 Orsay, France.
authors.forename: Carsten
authors.initials: C
authors.lastname: Janke
authors.affiliation: Institut Pasteur, Plateforme de Biophysique Moléculaire, CNRS UMR3528, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Raynal
authors.affiliation: Institut Pasteur, Unité de Biochimie des Interactions Macromoléculaires, CNRS UMR3528, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Chenal
authors.affiliation: INSERM, U1196, Université Paris Sud, Université Paris-Saclay, F-91405 Orsay, France.|Institut Curie, PSL Research University, CNRS UMR9187, F-91405 Orsay, France.
authors.forename: Sergio
authors.initials: S
authors.lastname: Marco
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR3348, F-91405 Orsay, France.|Université Paris Sud, Université Paris-Saclay, CNRS UMR3348, F-91405 Orsay, France.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Wehenkel
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D061327:Verrucomicrobia / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D008563:Membrane Lipids