title: Seasonal variations of carbonic anhydrase activity in Chongqing urban section of Jialing River and its influencing factors.
abstract: Carbonic anhydrase (CA) is an enzyme in algal carbon-utilization that plays an important role in the formation of algal blooms. A year-long monitoring program in the shore area of Chongqing Urban Section of the Jialing River (JR) was launched to determine the variations in carbonic anhydrase activity (CAA) and its change mechanism in the hydro-fluctuation belt of the tributaries in the Three Gorges Reservoir (TGR) area. The variations in basic water quality parameters, different carbon forms, and CAA were investigated from November 2013 to October 2014. Results showed that the mean CAA value in JR was 0.67 ± 0.31 EU/106cells. CAA was high during the flood stage, low during the impounding stage, and peaked on April 3, 2014 during the discharging stage. No significant difference was observed in the CAA of different sampling sites in JR. However, a significant difference was observed between the CAA of JR and that of the Yangtze River. Correlation analyses showed that water temperature, pH, algal cell density, and dissoluble organic carbon were positively correlated with CAA, whereas CO2and dissoluble inorganic carbon were negatively correlated with CAA. A model for CAA and related parameters was built through principal component regression. The equation was expressed as follows: CAA = 0.116T + 0.00746Cells+0.0156pH-0.0157CO2-0.0150DIC+0.0135DOC+0.565. Results revealed that CAA in JR was controlled by multiple factors, which could be used for CAA monitoring. The model demonstrated a potential value in controlling algal blooms.
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China. Electronic address: zhangzhicqu2016@126.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China.
authors.forename: Yinfan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Gao
authors.affiliation: Key Laboratory of Three Gorges Reservoir Region, Chongqing, 400045, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
mesh_terms: D002256:Carbonic Anhydrases / Q000378:metabolism*; D002681:China; D004784:Environmental Monitoring / Q000379:methods*; D005068:Eutrophication; D008962:Models, Theoretical; D045483:Rivers / Q000737:chemistry*; D012621:Seasons; D014507:Urbanization; D060753:Water Quality / Q000592:standards
chemical_list: D002256:Carbonic Anhydrases
keywords: Algal growth; Carbon form; Carbonic anhydrase activity; Hydro-fluctuation belt; Jialing River