title: High qualityin vitroexpansion of human endothelial progenitor cells of human umbilical vein origin.
abstract: The limited availability of qualified endothelial progenitor cells (EPCs) is a major challenge for regenerative medicine. In the present study, we isolated human EPCs from human umbilical vein endothelial cells (HUVECs) by using magnetic micro-beads coated with an antibody against human CD34. Flow cytometric assay showed that majority of these cells expressed VEGFR2 (KDR), CD34 and CD133, three molecular markers for early EPCs. It was also found that a bioreactor micro-carrier cell culture system (bio-MCCS) was superior to dish culture forin vitroexpansion of EPCs. It expanded more EPCs which were in the early stage, as shown by the expression of characteristic molecular markers and had better angiogenic potential, as shown by matrix-gel basedin vitroangiogenesis assay. These results suggest that HUVECs might be a novel promising resource of EPCs for regenerative medicine and that a bio-MCCS cell culture system might be broadly used forin vitroexpansion of EPCs.
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China;; The Second Hospital of Jilin University, Changchun, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mou
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yue
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China;; The First Hospital of Jilin University, Changchun, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Shi
authors.affiliation: The Second Hospital of Jilin University, Changchun, China.
authors.forename: Mingrui
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Chang
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Gao
authors.affiliation: Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China.
authors.forename: Ronggui
authors.initials: R
authors.lastname: Li
authors.affiliation: The Second Hospital of Jilin University, Changchun, China.
authors.forename: Zonggui
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000071916:AC133 Antigen / Q000096:biosynthesis; D018952:Antigens, CD34 / Q000096:biosynthesis; D019149:Bioreactors; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D005434:Flow Cytometry; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D044968:Regenerative Medicine; D014471:Umbilical Veins / Q000166:cytology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis
chemical_list: D000071916:AC133 Antigen; D018952:Antigens, CD34; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: angiogenesis; cell therapy.; endothelial progenitor cells; micro-carrier