title: Crystal structure of a small heat-shock protein from Xylella fastidiosa reveals a distinct high-order structure.
abstract: Citrus variegated chlorosis is a disease that attacks economically important citrus plantations and is caused by the plant-pathogenic bacterium Xylella fastidiosa. In this work, the structure of a small heat-shock protein from X. fastidiosa (XfsHSP17.9) is reported. The high-order structures of small heat-shock proteins from other organisms are arranged in the forms of double-disc, hollow-sphere or spherical assemblies. Unexpectedly, the structure reported here reveals a high-order architecture forming a nearly square cavity.
authors.affiliation: Laboratory of Structural Biology and Crystallography, Institute of Chemistry, University of Campinas, CP6154, 13083-970 Campinas-SP, Brazil.
authors.forename: Emanuella Maria Barreto
authors.initials: EM
authors.lastname: Fonseca
authors.affiliation: Laboratory of Structural Biology and Crystallography, Institute of Chemistry, University of Campinas, CP6154, 13083-970 Campinas-SP, Brazil.
authors.forename: Valéria
authors.initials: V
authors.lastname: Scorsato
authors.affiliation: Laboratory of Structural Biology and Crystallography, Institute of Chemistry, University of Campinas, CP6154, 13083-970 Campinas-SP, Brazil.
authors.forename: Marcelo Leite
authors.initials: ML
authors.lastname: Dos Santos
authors.affiliation: Molecular Biotechnology Group, Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics (IFSC), University of Sao Paulo (USP), Avenida Trabalhador São-carlense 400, Parque Arnold Schimidt, 13566-590 São Carlos-SP, Brazil.
authors.forename: Atilio Tomazini
authors.initials: AT
authors.lastname: Júnior
authors.affiliation: Laboratory of Molecular and Genetic Analysis, Center for Molecular Biology and Genetic Engineering, Institute of Biology, University of Campinas, CP6010, 13083-875 Campinas-SP, Brazil.
authors.forename: Susely Ferraz Siqueira
authors.initials: SF
authors.lastname: Tada
authors.affiliation: Laboratory of Molecular and Genetic Analysis, Center for Molecular Biology and Genetic Engineering, Institute of Biology, University of Campinas, CP6010, 13083-875 Campinas-SP, Brazil.
authors.forename: Clelton Aparecido
authors.initials: CA
authors.lastname: Dos Santos
authors.affiliation: Laboratory of Molecular and Genetic Analysis, Center for Molecular Biology and Genetic Engineering, Institute of Biology, University of Campinas, CP6010, 13083-875 Campinas-SP, Brazil.
authors.forename: Marcelo Augusto Szymanski
authors.initials: MA
authors.lastname: de Toledo
authors.affiliation: Laboratory of Molecular and Genetic Analysis, Center for Molecular Biology and Genetic Engineering, Institute of Biology, University of Campinas, CP6010, 13083-875 Campinas-SP, Brazil.
authors.forename: Anete Pereira
authors.initials: AP
authors.lastname: de Souza
authors.affiliation: Molecular Biotechnology Group, Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics (IFSC), University of Sao Paulo (USP), Avenida Trabalhador São-carlense 400, Parque Arnold Schimidt, 13566-590 São Carlos-SP, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
authors.affiliation: Laboratory of Structural Biology and Crystallography, Institute of Chemistry, University of Campinas, CP6154, 13083-970 Campinas-SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Aparicio
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D050888:Heat-Shock Proteins, Small / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D040681:Structural Homology, Protein; D044167:Xylella / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D050888:Heat-Shock Proteins, Small; C113725:HspA protein, bacteria; D011994:Recombinant Proteins
keywords: XfsHSP17.9; Xylella fastidiosa; chaperones; citrus variegated chlorosis; small heat-shock protein; α-crystallin domain