title: Direct labeling of serum proteins by fluorescent dye for antibody microarray.
abstract: Analysis of serum proteome by antibody microarray is used to identify novel biomarkers and to study signaling pathways including protein phosphorylation and protein-protein interactions. Labeling of serum proteins is important for optimal performance of the antibody microarray. Proper choice of fluorescent label and optimal concentration of protein loaded on the microarray ensure good quality of imaging that can be reliably scanned and processed by the software. We have optimized direct serum protein labeling using fluorescent dye Arrayit Green 540 (Arrayit Corporation, USA) for antibody microarray. Optimized procedure produces high quality images that can be readily scanned and used for statistical analysis of protein composition of the serum.
authors.affiliation: National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation.
authors.forename: M V
authors.initials: MV
authors.lastname: Klimushina
authors.affiliation: National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation. Electronic address: gumanova@mail.ru.
authors.forename: N G
authors.initials: NG
authors.lastname: Gumanova
authors.affiliation: National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation.
authors.forename: V A
authors.initials: VA
authors.lastname: Metelskaya
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002232:Carbocyanines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000906:Antibodies; D001798:Blood Proteins; D002232:Carbocyanines; D005456:Fluorescent Dyes
keywords: Antibody; Fluorophore; Microarray; Protein labeling