title: PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations.
abstract: To fabricate PEGylated liposomes which preserve the activity of hydrophobic Wnt3A protein, and to demonstrate their efficacy in promoting expansion of osteoprogenitors from human bone marrow.
  
  
  
  PEGylated liposomes composed of several synthetic lipids were tested for their ability to preserve Wnt3A activity in reporter and differentiation assays. Single-molecule microspectroscopy was used to test for direct association of protein with liposomes.
  
  
  
  Labeled Wnt3A protein directly associated with all tested liposome preparations. However, Wnt3A activity was preserved or enhanced in PEGylated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes but not in PEGylated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes. PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis.
  
  
  
  Active Wnt protein-containing PEGylated liposomes may have utility for systemic administration for bone repair.
authors.affiliation: Human Development & Health Academic Unit, Faculty of Medicine, Centre for Human Development, Stem Cells & Regeneration, Bone & Joint Research Group & Institute for Life Sciences, Institute for Developmental Sciences, University of Southampton, Tremona Road, Southampton, SO16 6YD, UK.
authors.forename: Agnieszka A
authors.initials: AA
authors.lastname: Janeczek
authors.affiliation: Human Development & Health Academic Unit, Faculty of Medicine, Centre for Human Development, Stem Cells & Regeneration, Bone & Joint Research Group & Institute for Life Sciences, Institute for Developmental Sciences, University of Southampton, Tremona Road, Southampton, SO16 6YD, UK.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Scarpa
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK.
authors.forename: Mathew H
authors.initials: MH
authors.lastname: Horrocks
authors.affiliation: Human Development & Health Academic Unit, Faculty of Medicine, Centre for Human Development, Stem Cells & Regeneration, Bone & Joint Research Group & Institute for Life Sciences, Institute for Developmental Sciences, University of Southampton, Tremona Road, Southampton, SO16 6YD, UK.
authors.forename: Rahul S
authors.initials: RS
authors.lastname: Tare
authors.affiliation: Microbiology Group, Chemical, Biological & Radiological Division, Dstl, Porton Down, Salisbury, SP4 0JQ, UK.
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Rowland
authors.affiliation: Microbiology Group, Chemical, Biological & Radiological Division, Dstl, Porton Down, Salisbury, SP4 0JQ, UK.
authors.forename: Dominic
authors.initials: D
authors.lastname: Jenner
authors.affiliation: Clinical & Experimental Sciences, Faculty of Medicine, Institute for Life Sciences, University of Southampton, Building 85, Life Sciences Building, Highfield Campus, Southampton, SO17 1BJ, UK.
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Newman
authors.affiliation: Human Development & Health Academic Unit, Faculty of Medicine, Centre for Human Development, Stem Cells & Regeneration, Bone & Joint Research Group & Institute for Life Sciences, Institute for Developmental Sciences, University of Southampton, Tremona Road, Southampton, SO16 6YD, UK.
authors.forename: Richard Oc
authors.initials: RO
authors.lastname: Oreffo
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK.
authors.forename: Steven F
authors.initials: SF
authors.lastname: Lee
authors.affiliation: Human Development & Health Academic Unit, Faculty of Medicine, Centre for Human Development, Stem Cells & Regeneration, Bone & Joint Research Group & Institute for Life Sciences, Institute for Developmental Sciences, University of Southampton, Tremona Road, Southampton, SO16 6YD, UK.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Evans
mesh_terms: D001854:Bone Marrow Cells / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry; D013234:Stem Cells / Q000187:drug effects; D060509:Wnt3A Protein / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D060509:Wnt3A Protein; D011092:Polyethylene Glycols; C017251:1,2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine
keywords: PEGylated liposomes; Wnt; fracture repair; liposomes; marrow stromal cells; mesenchymal stem cells; osteoprogenitors; regenerative medicine; skeletal stem cells