title: Identification of a Ribose-Phosphate Pyrophosphokinase that Can Interact In Vivo with the Anaphase Promoting Complex/Cyclosome.
abstract: 5-Phospho-d-ribosyl-1-diphosphate (PRPP) synthase (PRS) catalyzes the biosynthesis of PRPP, which is an important compound of metabolism in most organisms. However, noPRSgenes have been cloned, let alone studied for their biological function in rubber tree. In this study, we identify a novel protein (PRS4) that interacts in vivo with rubber tree anaphase promoting complex/cyclosome (APC/C) subunit 10 (HbAPC10) by yeast two-hybrid assays.PRS4has been cloned from rubber tree and named asHbPRS4. Blastp search in the genome ofArabidopsis thalianashowed that HbPRS4 shared the highest similarity with AtPRS4, with 80.71% identity. qRT-PCR was used to determine the expression ofHbPRS4in different tissues and under various treatments.HbPRS4was preferentially expressed in the bark. Moreover, the expression level ofHbPRS4was significantly induced by the proteasome inhibitor MG132 treatment, suggesting it might be regulated by the ubiquitin/26S proteasome pathway. The amount ofHbPRS4transcript was obviously decreased after mechanical wounding and abscisic acid (ABA) treatments, while a slight increase was observed at 24 h after ABA treatment.HbPRS4transcript in the latex was significantly upregulated by ethephon (ET) and methyl jasmonate (MeJA) treatments. These results suggested that HbPRS4 may be a specific substrate of HbAPC10 indirectly regulating natural rubber biosynthesis in rubber tree.
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. yuhaiyang@hainu.edu.cn.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. yuzhang_rain@hainu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. zhangdong19921@hotmail.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. luyanxi3@outlook.com.
authors.forename: Yanxi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. hhxn1991@hotmail.com.
authors.forename: Haixia
authors.initials: H
authors.lastname: He
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. zfucong@outlook.com.
authors.forename: Fucong
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. wangmeng@hainu.edu.cn.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D028482:Hevea / Q000235:genetics / Q000378:metabolism; D007976:Leupeptins / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology; D024301:Plant Bark / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012268:Ribose-Phosphate Pyrophosphokinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D007976:Leupeptins; D054883:Oxylipins; D010940:Plant Proteins; D000040:Abscisic Acid; C072239:methyl jasmonate; D064173:Anaphase-Promoting Complex-Cyclosome; D012268:Ribose-Phosphate Pyrophosphokinase; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde
keywords: ">d-ribosyl-1-diphosphate; 5-phospho-; anaphase promoting complex/cyclosome; ribose-phosphate pyrophosphokinase; rubber tree