title: Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli.
abstract: We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs and microemboli by 1.6 and 3.0-fold, respectively (both P < 0.05).
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw and Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Chian-Hui
authors.identifier: http://orcid.org/0000-0002-2697-4845
authors.initials: CH
authors.lastname: Lai
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw.
authors.forename: Syer
authors.initials: S
authors.lastname: Choon Lim
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw.
authors.forename: Liang-Chun
authors.initials: LC
authors.lastname: Wu
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw.
authors.forename: Chien-Fang
authors.initials: CF
authors.lastname: Wang
authors.affiliation: Graduate Institute of Clinical Medical Science, Chang-Gung University, Taoyuan, Taiwan and Division of Colon and Rectal Surgery, Chang-Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Wen-Sy
authors.initials: WS
authors.lastname: Tsai
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Han-Chung
authors.initials: HC
authors.lastname: Wu
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw and Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
authors.forename: Ying-Chih
authors.initials: YC
authors.lastname: Chang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D015179:Colorectal Neoplasms / Q000276:immunology / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020766:Intracranial Embolism / Q000276:immunology / Q000473:pathology*; D046210:Microfluidic Analytical Techniques; D008968:Molecular Conformation; D009360:Neoplastic Cells, Circulating / Q000276:immunology / Q000473:pathology*
chemical_list: D055910:Antibodies, Immobilized