title: Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal.
abstract: In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistantStaphylococcus aureusisolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC) of oxacillin. 264 (59%) samples were culture positive. The highest incidence of bacterial infections was noted in the age group of less than 1 year (76%). Out of 264 growth positive samples, Gram-positive bacteria were isolated from 162 (61%) samples and Gram-negative bacteria were found in 102 (39%) samples.Staphylococcus aureus(99%) was the predominant Gram-positive bacteria isolated andPseudomonas aeruginosa(44%) was predominant Gram-negative bacteria. About 19% ofS. aureusisolates were found to be methicillin-resistant MIC of oxacillin ranging from 4μg/mL to 128μg/mL. Among the children of Nepal, those of age less than 1 year were at higher risk of wound infections by bacteria.Staphylococcus aureusfollowed byPseudomonas aeruginosawere the most common bacteria causing wound infections in children.
authors.affiliation: Department of Microbiology, Goldengate International College, Kathmandu, Nepal.
authors.forename: Salu
authors.initials: S
authors.lastname: Rai
authors.affiliation: Forum for Health Research and Development, Dharan, Nepal.
authors.forename: Uday Narayan
authors.initials: UN
authors.lastname: Yadav
authors.affiliation: Department of Microbiology, Grande International Hospital, Kathmandu, Nepal.
authors.forename: Narayan Dutt
authors.identifier: 0000-0002-7577-2633
authors.initials: ND
authors.lastname: Pant
authors.affiliation: Central Campus of Technology, Hattisar, Dharan, Nepal.
authors.forename: Jaya Krishna
authors.initials: JK
authors.lastname: Yakha
authors.affiliation: Department of Microbiology, Kanti Children's Hospital, Kathmandu, Nepal.
authors.forename: Prem Prasad
authors.initials: PP
authors.lastname: Tripathi
authors.affiliation: Department of Microbiology, Goldengate International College, Kathmandu, Nepal.
authors.forename: Asia
authors.initials: A
authors.lastname: Poudel
authors.affiliation: Department of Microbiology, Goldengate International College, Kathmandu, Nepal.
authors.forename: Binod
authors.initials: B
authors.lastname: Lekhak