title: A multifunctional bioactive material that stimulates osteogenesis and promotes the vascularization bone marrow stem cells and their resistance to bacterial infection.
abstract: The main limitation of tissue engineering lies in the inability to stimulate osteogenesis, angiogenesis of stem cells and broad-spectrum antimicrobial activity. However, the development of multifunctional bioactive materials with these capabilities remains a great challenge. In this study, we prepared mesoporous silica nanoparticles encapsulated with silver nanocrystals (AG-MSN) with uniform sphere size and mesopores. Platelet-derived growth factor BB (PDGF-BB) was effectively loaded in the AG-MSN mesopores (P-AG-MSN). The silicon ions (Si) released by P-AG-MSN stimulate osteogenic differentiation of bone marrow stromal cells (BMSC) by activating the alkaline phosphatase (ALP) activity of bone-related genes and increasing protein (OCN, RUNX2 and OPN) expression. Ag+ ions could be slowly released from the interior of the shell, highlighting their durable antibacterial activity. The sustained release of PDGF-BB from P-AG-MSN stimulated the angiogenic differentiation of BMSC, as indicated by the enhanced secretion of vascular endothelial growth factor (VEGF), HIF-1α, HGF and ANG-1 and protein expression. Our results show that P-AG-MSN can clearly promote BMSC osteostimulation and vascularization. This research serves as a preliminary study of the utilization of this multifunctional mixture to fabricate a new active biological scaffold that integrates BMSC osteostimulation, vascularization and bactericidal effects by 3D printing technology.
authors.affiliation: Department of Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.|Department of Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University Chang Ji Branch, Chang Ji, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Ma
authors.affiliation: Xinjiang Key Laboratory of Medical Animal Model Research, Clinical Medical Research Institute of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cao
authors.affiliation: Carders Health Care No. 4 Department of Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, China.
authors.forename: Xinchun
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Clinical Laboratory, The first people's Hospital of Urumqi, Urumqi, China.
authors.forename: Juling
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Science and Research Education Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Pu
authors.affiliation: Department of Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Aihemaitijiang
authors.initials: A
authors.lastname: Yusufu
authors.affiliation: Department of Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
mesh_terms: D001424:Bacterial Infections / Q000188:drug therapy* / Q000382:microbiology; D000077214:Becaplermin; D001854:Bone Marrow Cells / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D066330:Printing, Three-Dimensional; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage* / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis; D012822:Silicon Dioxide / Q000737:chemistry; D013234:Stem Cells / Q000187:drug effects*; D023822:Tissue Engineering; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: C066855:HGF protein, human; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin; D017228:Hepatocyte Growth Factor; D012822:Silicon Dioxide; C046366:angiogenin; D012259:Ribonuclease, Pancreatic