title: Non-genetic impact factors on chronological lifespan and stress resistance of baker's yeast.
abstract: Survival under nutrient limitation is an essential feature of microbial cells, and it is defined by the chronological lifespan. We summarize recent findings, illustrating how crucial the choice of the experimental setup is for the interpretation of data in this field. Especially the impact of oxygen supply differs depending on the culture type, highlighting the differences of alternatives like the retentostat to classical batch cultures. Finally the importance of culture conditions on cell aging and survival in biotechnological processes is highlighted.
authors.affiliation: Department of Biotechnology, BOKU - VIBT, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria. ; Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Sauer
authors.affiliation: Department of Biotechnology, BOKU - VIBT, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria. ; Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Diethard
authors.initials: D
authors.lastname: Mattanovich
keywords: Yeast; aging; chronological lifespan; oxygen; stationary culture