title: An investigation on cytotoxic effect of bioactive AgNPs synthesized usingCassia fistulaflower extract on breast cancer cell MCF-7.
abstract: A single step protocol to produce biofunctionalized silver nanoparticles (AgNPs) using the aqueous extract ofCassia fistulaflower as "natural factory" was investigated. The reaction between silver ions and aqueous flower extract after the bioreduction process has resulted in the formation of reddish brown color colloidal solution. XRD pattern showed the face centered cubic crystalline structure of AgNPs and exhibited spherical morphology as characterized by FE-SEM. FTIR studies identified different functional groups involved in effective capping of AgNPs. Thezeta potentialaffirmed the phytoreduced AgNPs possess good stability and the size of the particle was measured by DLS. The synthesized AgNPs displayed effective cytotoxic potential against MCF7 and the inhibitory concentration (IC50) was recorded at 7.19 μg/mL. The apoptotic effects of the AgNPs were also confirmed by AO/EB staining. The investigation presents preliminary evidence that biosynthesized AgNPs can be used in the development of novel anticancer drugs.
authors.affiliation: Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamilnadu, India.
authors.forename: R R
authors.initials: RR
authors.lastname: Remya
authors.affiliation: Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamilnadu, India.
authors.forename: S R Radhika
authors.initials: SR
authors.lastname: Rajasree
authors.affiliation: Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamilnadu, India.
authors.forename: L
authors.initials: L
authors.lastname: Aranganathan
authors.affiliation: Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamilnadu, India.
authors.forename: T Y
authors.initials: TY
authors.lastname: Suman
keywords: Cassia fistula; Cytotoxicity; Silver nanoparticles