title: Detection Method for Aquatic Bacteria of the Fingers, as a Potential Origin of the Aqueous Solution Contamination.
abstract: Aquatic bacteria were isolated from the hands of working staffs by an adapted culture protocol. When the sample solution obtained by the" glove juice method" was incubated for 3 days at room temperature, viable cell counts increased up to 105-fold, and the majority of the isolated colonies were shown to be Gram-negative aquatic bacteria, which carry the risk of contaminating water. Using R2A medium, coagulase-negative staphylococci were the dominant microbes immediately after recovery from the hands. Here it was revealed that bacteria of the phylum Proteobacteria isolated from the hand can be the causative bacteria of aqueous contamination. This modification in the GJ method may be useful as an effective training protocol to demonstrate the importance of hand hygiene and clean operation for aseptic manufacturing.
authors.affiliation: Koshigaya Ohbukuro Clinic.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Osono
authors.forename: Kazumi
authors.initials: K
authors.lastname: Honda
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inoue
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Norose
authors.forename: Megumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ichimura
authors.forename: Chisako
authors.initials: C
authors.lastname: Kamano
authors.forename: Eiji
authors.initials: E
authors.lastname: Shinya
authors.forename: Shun
authors.initials: S
authors.lastname: Takaku
authors.forename: Kentaro
authors.initials: K
authors.lastname: Okamatsu
authors.forename: Shinya
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takizawa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058491:Bacterial Load; D005385:Fingers / Q000382:microbiology*; D063373:Hand Hygiene; D006801:Humans; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012996:Solutions
chemical_list: D012336:RNA, Ribosomal, 16S; D012996:Solutions