title: A farnesoic acid-responsive transcription factor, Hot1, regulates yeast-hypha morphogenesis in Candida albicans.
abstract: Candida albicans hyphal formation is inhibited by a quorum-sensing molecule, farnesoic acid, which accumulates in the medium as the cells proliferate. We recently showed that Pho81 is essential for the inhibition of hyphal growth by farnesoic acid. Here, we describe a newly identified regulator, Hot1, which increases the expression of PHO81. The binding site of Hot1 in the PHO81 promoter region was identified by DNase I protection assay. The hot1Δ mutant grows extensively as filaments. Furthermore, the inhibition of hyphal formation and the repression of major signaling pathway components in response to farnesoic acid are defective in hot1Δ mutant cells. These data suggest an important role for HOT1 in the inhibition of hyphal development by farnesoic acid in this fungus.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Chan-Hong
authors.initials: CH
authors.lastname: Ahn
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Sohyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Eunji
authors.initials: E
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Heegyu
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Beomkoo
authors.initials: B
authors.lastname: Chung
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Wanki
authors.initials: W
authors.lastname: Park
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Korea.
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Korea.
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D025301:Hyphae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009024:Morphogenesis / Q000187:drug effects / Q000235:genetics; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D005656:Fungal Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C007702:farnesoic acid
keywords: 
  Candida albicans
  ; Hot1; farnesoic acid