title: Biosafety Level 3 setup for multiphoton microscopy in vivo.
abstract: Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method that renders multiphoton microscopy in vivo compatible with biosafety level 3 regulations and present an example of its application and potential to visualize a Mycobacterium tuberculosis infection of the mouse lung.
authors.affiliation: EuroBioconcept SAS, 94380, Bonneuil sur Marne, France.
authors.forename: D
authors.initials: D
authors.lastname: Barlerin
authors.affiliation: EuroBioconcept SAS, 94380, Bonneuil sur Marne, France.
authors.forename: G
authors.initials: G
authors.lastname: Bessière
authors.affiliation: Centre National de la Recherche Scientifique (CNRS), Institut de Pharmacologie et de Biologie Structurale (IPBS), 31000, Toulouse, France.|Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale (IPBS), 31000, Toulouse, France.
authors.forename: J
authors.initials: J
authors.lastname: Domingues
authors.affiliation: Lavision Biotech GmbH, 33617, Bielefeld, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schuette
authors.affiliation: Lavision Biotech GmbH, 33617, Bielefeld, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Feuillet
authors.affiliation: Centre National de la Recherche Scientifique (CNRS), Institut de Pharmacologie et de Biologie Structurale (IPBS), 31000, Toulouse, France. antonio.peixoto@ipbs.fr.|Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale (IPBS), 31000, Toulouse, France. antonio.peixoto@ipbs.fr.
authors.forename: A
authors.initials: A
authors.lastname: Peixoto
mesh_terms: D003264:Containment of Biohazards / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D019983:Guideline Adherence; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods / Q000592:standards