title: Assessment of contrast-enhanced ultrasonography of the hepatic vein for detection of hemodynamic changes associated with experimentally induced portal hypertension in dogs.
abstract: OBJECTIVE To assess the use of contrast-enhanced ultrasonography (CEUS) of the hepatic vein for the detection of hemodynamic changes associated with experimentally induced portal hypertension in dogs. ANIMALS 6 healthy Beagles. PROCEDURES A prospective study was conducted. A catheter was surgically placed in the portal vein of each dog. Hypertension was induced by intraportal injection of microspheres (10 to 15 mg/kg) at 5-day intervals via the catheter. Microsphere injections were continued until multiple acquired portosystemic shunts were created. Portal vein pressure (PVP) was measured through the catheter. Contrast-enhanced ultrasonography was performed before and after establishment of hypertension. Time-intensity curves were generated from the region of interest in the hepatic vein. Perfusion variables measured for statistical analysis were hepatic vein arrival time, time to peak, time to peak phase (TTPP), and washout ratio. The correlation between CEUS variables and PVP was assessed by use of simple regression analysis. RESULTS Time to peak and TTPP were significantly less after induction of portal hypertension. Simple regression analysis revealed a significant negative correlation between TTPP and PVP. CONCLUSIONS AND CLINICAL RELEVANCE CEUS was useful for detecting hemodynamic changes associated with experimentally induced portal hypertension in dogs, which was characterized by a rapid increase in the intensity of the hepatic vein. Furthermore, TTPP, a time-dependent variable, provided useful complementary information for predicting portal hypertension. IMPACT FOR HUMAN MEDICINE Because the method described here induced presinusoidal portal hypertension, these results can be applied to idiopathic portal hypertension in humans.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Morishita
authors.forename: Akira
authors.initials: A
authors.lastname: Hiramoto
authors.forename: Asuka
authors.initials: A
authors.lastname: Michishita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takagi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Takaharu
authors.initials: T
authors.lastname: Itami
authors.forename: Sue Yee
authors.initials: SY
authors.lastname: Lim
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Osuga
authors.forename: Sayuri
authors.initials: S
authors.lastname: Nakamura
authors.forename: Kenji
authors.initials: K
authors.lastname: Ochiai
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Takiguchi
mesh_terms: D000818:Animals; D003287:Contrast Media; D004283:Dog Diseases / Q000000981:diagnostic imaging*; D004285:Dogs; D006439:Hemodynamics; D006503:Hepatic Veins / Q000000981:diagnostic imaging*; D006801:Humans; D006975:Hypertension, Portal / Q000000981:diagnostic imaging / Q000662:veterinary*; D008103:Liver Cirrhosis; D008297:Male; D008863:Microspheres; D010198:Pancytopenia; D010477:Perfusion; D011169:Portal Vein; D011446:Prospective Studies; D013163:Splenomegaly; D014463:Ultrasonography / Q000379:methods / Q000662:veterinary*; D000094724:Idiopathic Noncirrhotic Portal Hypertension
chemical_list: D003287:Contrast Media