title: Identification of conserved proteins from diverse shell matrix proteome in Crassostrea gigas: characterization of genetic bases regulating shell formation.
abstract: The calcifying shell is an excellent model for studying biomineralization and evolution. However, the molecular mechanisms of shell formation are only beginning to be elucidated in Mollusca. It is known that shell matrix proteins (SMPs) play important roles in shell formation. With increasing data of shell matrix proteomes from various species, we carried out a BLASTp bioinformatics analysis using the shell matrix proteome from Crassostrea gigas against 443 SMPs from nine other species. The highly conserved tyrosinase and chitin related proteins were identified in bivalve. In addition, the relatively conserved proteins containing domains of carbonic anhydrase, Sushi, Von Willebrand factor type A, and chitin binding, were identified from all the ten species. Moreover, 25 genes encoding SMPs were annotated and characterized that are involved in CaCO3crystallization and represent chitin related or ECM related proteins. Together, data from these analyses provide new knowledge underlying the molecular mechanism of shell formation in C.gigas, supporting a refined shell formation model including chitin and ECM-related proteins.
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Feng
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Kong
authors.affiliation: Institute of Marine and Environmental Technology, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, United States.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Du
mesh_terms: D060105:Animal Shells / Q000378:metabolism*; D000818:Animals; D049872:Bivalvia / Q000235:genetics / Q000378:metabolism; D002113:Calcification, Physiologic / Q000235:genetics; D049896:Crassostrea / Q000235:genetics* / Q000378:metabolism*; D049851:Gastropoda / Q000235:genetics / Q000378:metabolism; D007448:Invertebrates / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D020543:Proteome / Q000235:genetics*
chemical_list: D020543:Proteome