title: Identification of novel cancer fusion genes using chromosome breakpoint screening.
abstract: Gene fusion due to rearrangement or translocation of chromosomes is a powerful mutational mechanism during tumorigenesis. Several new high-resolution technologies have recently been developed to evaluate large numbers of small aberrations as candidate loci for fusion gene screening. In our previous whole-genome screening study using 500K SNP arrays, we identified more than 700 homozygous deletions (HDs) and amplicons in 23 cancer cell lines. To explore novel fusion genes in cancer, we established stringent criteria for defining HD and amplicon breakpoints. Then genomic PCR and sequencing analyses identified a fusion gene, FNDC3B-PRKCI, that resulted from chromosome intra-rearrangement. Western blotting and 3'-RACE analyses revealed that the chimeric transcript was an in-frame fusion between FNDC3B and PRKCI. Finally, cell migration and colony formation assays suggested that FNDC3B-PRKCI is a potential oncogene.
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan, R.O.C.
authors.forename: Kate
authors.initials: K
authors.lastname: Hua
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan, R.O.C.
authors.forename: Chin-Hui
authors.initials: CH
authors.lastname: Lin
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan, R.O.C.
authors.forename: Ya-Lun
authors.initials: YL
authors.lastname: Chen
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan, R.O.C.
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department and Institute of Pharmacology, National Yang-Ming University, Taipei 112, Taiwan, R.O.C.
authors.forename: Yueh-Hsin
authors.initials: YH
authors.lastname: Ping
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, R.O.C.
authors.forename: Yuh-Shan
authors.initials: YS
authors.lastname: Jou
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan, R.O.C.
authors.forename: Chian-Feng
authors.initials: CF
authors.lastname: Chen
mesh_terms: D056905:Chromosome Breakpoints; D005353:Fibronectins / Q000235:genetics; D055106:Genome-Wide Association Study / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011493:Protein Kinase C / Q000235:genetics; D017384:Sequence Deletion; D014178:Translocation, Genetic; D000099293:Protein Kinase C-lambda
chemical_list: D005353:Fibronectins; D007527:Isoenzymes; D015514:Oncogene Proteins, Fusion; D011493:Protein Kinase C; D000099293:Protein Kinase C-lambda; C543006:FNDC3B protein, human