title: 3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells.
abstract: The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany. Electronic address: mutsenko@imp.uni-hannover.de.
authors.forename: Vitalii V
authors.initials: VV
authors.lastname: Mutsenko
authors.affiliation: Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany.
authors.forename: Vasilii V
authors.initials: VV
authors.lastname: Bazhenov
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine.
authors.forename: Olena
authors.initials: O
authors.lastname: Rogulska
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine.
authors.forename: Dmitriy N
authors.initials: DN
authors.lastname: Tarusin
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Schütz
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Sophie
authors.initials: S
authors.lastname: Brüggemeier
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Gossla
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Ashwini R
authors.initials: AR
authors.lastname: Akkineni
authors.affiliation: Department of Prosthetic Dentistry, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Meißner
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Lode
authors.affiliation: BromMarin GmbH, Wernerstraße 1, 09599 Freiberg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Meschke
authors.affiliation: BromMarin GmbH, Wernerstraße 1, 09599 Freiberg, Germany.
authors.forename: Andre
authors.initials: A
authors.lastname: Ehrlich
authors.affiliation: Institute of Marine Biology, Institute of marine biology, University of Montenegro, 85330 Kotor, Montenegro.
authors.forename: Slavica
authors.initials: S
authors.lastname: Petović
authors.affiliation: Institute of Marine Biology, Institute of marine biology, University of Montenegro, 85330 Kotor, Montenegro.
authors.forename: Rajko
authors.initials: R
authors.lastname: Martinović
authors.affiliation: Institute of Marine Biology, Institute of marine biology, University of Montenegro, 85330 Kotor, Montenegro.
authors.forename: Mirko
authors.initials: M
authors.lastname: Djurović
authors.affiliation: Department of Biochemistry, Duke University School of Medicine, 27710 Durham, North Carolina, USA.
authors.forename: Allison L
authors.initials: AL
authors.lastname: Stelling
authors.affiliation: Moscow Institute of Physics and Technology, Institutskii Per. 9, 141700, Dolgoprudny, Moscow Region, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Nikulin
authors.affiliation: P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p. 3, 125284 Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Rodin
authors.affiliation: P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p. 3, 125284 Moscow, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tonevitsky
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Petrenko
authors.affiliation: Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Glasmacher
authors.affiliation: Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany. Electronic address: Hermann.Ehrlich@physik.tu-freiberg.de.
authors.forename: Hermann
authors.initials: H
authors.lastname: Ehrlich
mesh_terms: D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002686:Chitin / Q000737:chemistry; D020219:Chondrogenesis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D010012:Osteogenesis; D011161:Porifera / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D002686:Chitin
keywords: Chitin scaffolds; Mesenchymal stromal cells; Sponges