title: Promising anticancer activity of Cyclotrichium niveum L. extracts through induction of both apoptosis and necrosis.
abstract: In this study, cytotoxic effects of the dichloromethane, ethyl acetate, ethanol, and aqueous extracts of the aerial parts of Cyclotrichium niveum (Boiss.) Manden. & Scheng (Lamiaceae) were evaluated. We tested HeLa, MCF-7 cancer cells, and MRC-5 and MCF-10A normal cells. The ethyl acetate and dichloromethane extracts induced cytotoxicity whereas the ethanol and aqueous extracts had no cytotoxic activity against both cancer cells. IC50values of the dichloromethane extract were 353.0 ± 84.30 μg/ml, 114.8 ± 40.34 μg/ml, 39 ± 0.56 μg/ml, and 49 ± 13 μg/ml in HeLa, MCF-7, MRC-5, MCF-10A cells, respectively. IC50values of the ethyl acetate extract were 117.0 ± 36.24 μg/ml in HeLa cells, 156.3 ± 19.86 μg/ml in MCF-7 cells, 1100 ± 340 μg/ml in MRC-5 cells and 7900 ± 1200 μg/ml in MCF-10A cells. Additionally, the ethyl acetate extract showed more selectivity to HeLa and MCF-7 cancer cells than MRC-5 and MCF-10A normal cells. Our results of HPLC analysis showed that apigenin in the ethyl acetate extract (2.2518 ± 0.0005 mg/100 mg extract) might be responsible of that selective cytotoxic effect. In the current work, we have shown for the first time that C. niveum has cytotoxic properties in the cancer cell lines tested.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Özdemir
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Yildiz
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Fatma Sezer
authors.initials: FS
authors.lastname: Senol
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Yaprak Dilber
authors.initials: YD
authors.lastname: Şimay
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Burçin
authors.initials: B
authors.lastname: Ibişoglu
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Gokbulut
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Ilkay Erdogan
authors.initials: IE
authors.lastname: Orhan
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey. Electronic address: mark@gazi.edu.tr.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ark
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D061986:MCF-7 Cells; D009336:Necrosis / Q000188:drug therapy / Q000503:physiopathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
keywords: Apigenin; Cancer; Cyclotrichium niveum; Cytotoxicity; HPLC; Lamiaceae