title: Multiresidue determination of veterinary medicines in lyophilized egg albumen with subsequent consumer exposure evaluation.
abstract: The process of lyophilization causes that the veterinary drugs residues present in egg albumen do not decompose, as it takes place during the process of high-temperature drying. Thus, lyophilized albumen may be a potential source of their residues for consumers. As a consequence, reliable methods for the determination of veterinary medicinal products from egg albumen are needed. The method for the determination of 85 analytes in lyophilized egg albumen was developed and successfully validated. The recoveries were between 84 and 110%, within laboratory repeatability and reproducibility - in the range of 3.29-16.8% and -5.93 to 19.3%. The presence of enrofloxacin and doxycycline was confirmed in real egg albumen samples. The concentrations ranged from 5.65-596µg/kg for doxycycline to 0.89-134µg/kg for enrofloxacin. Nevertheless, the evaluated daily intake and % of the ADI (Acceptable Daily Intake) received by the consumers' were at a toxicologically accepted level.
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland. Electronic address: marta.piatkowska@piwet.pulawy.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Piątkowska
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Gbylik-Sikorska
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Gajda
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Jedziniak
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Błądek
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Żmudzki
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Posyniak
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D000418:Albumins
keywords: Coccidiostats; Doxycycline (CAS 24390-14-5); Egg albumen; Enrofloxacin (CAS 93106-60-6); LC/MS/MS; Residues; Veterinary medicines