title: Monitoring Migration of Activated T Cells to Antigen-Rich Non-lymphoid Tissue.
abstract: Effective immunity requires appropriate recirculation of naïve T cells through secondary lymphoid organs and migration of antigen-specific T cells to sites of inflammation. Leukocyte migration is a highly regulated process requiring specific interactions between leukocytes and endothelial cells (EC) termed collectively as the leukocyte adhesion cascade. Recruitment and retention of activated T cells to antigen-rich sites of inflammation is a key event in the immune response, which relies in part on local antigen presentation particularly by EC of inflamed vessels. Here we describe methods to assess the contributions of different molecules on antigen-dependent T cell migration, by utilizing IFN-γ to upregulate MHC molecules on EC and local antigen presentation, both in vitro and in vivo.
authors.affiliation: William Harvey Research Institute-Heart Centre Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London, EC1M 6BQ, UK.
authors.forename: Eleanor Jayne
authors.initials: EJ
authors.lastname: Ward
authors.affiliation: William Harvey Research Institute-Heart Centre Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London, EC1M 6BQ, UK.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Fu
authors.affiliation: William Harvey Research Institute-Heart Centre Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London, EC1M 6BQ, UK. f.marelli-berg@qmul.ac.uk.
authors.forename: Federica
authors.initials: F
authors.lastname: Marelli-Berg
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D004730:Endothelium, Vascular / Q000276:immunology; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000941:Antigens
keywords: Antigen; Endothelial cells; T lymphocytes; Transendothelial migration