title: Insights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptome.
abstract: The anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish norXenopusis electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish (Polyodon spathula). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Cavchannel and rectifying Kvchannel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution.
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Melinda S
authors.initials: MS
authors.lastname: Modrell
authors.affiliation: Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Mike
authors.initials: M
authors.lastname: Lyne
authors.affiliation: Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Carr
authors.affiliation: Department of Neuroscience, The University of Texas at Austin, Austin, United States.|Department of Integrative Biology, The University of Texas at Austin, Austin, United States.
authors.forename: Harold H
authors.initials: HH
authors.lastname: Zakon
authors.affiliation: Departmento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales-MNCN-CSIC, Madrid, Spain.|Department of Natural Sciences, Saint Louis University - Madrid Campus, Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Buckley
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Campbell
authors.affiliation: Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, United States.
authors.forename: Marcus C
authors.identifier: 0000-0002-2462-0138
authors.initials: MC
authors.lastname: Davis
authors.affiliation: Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Gos
authors.identifier: 0000-0002-6883-6168
authors.initials: G
authors.lastname: Micklem
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Clare Vh
authors.identifier: 0000-0002-4434-3107
authors.initials: CV
authors.lastname: Baker
mesh_terms: D000825:Animal Structures / Q000196:embryology*; D000818:Animals; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017423:Sequence Analysis, RNA; D014714:Vertebrates / Q000196:embryology*
keywords: Atoh1; Cav1.3; Cavβ2; Kv1.5; Kvβ3; Neurod4; Polyodon spathula (Mississippi paddlefish); Pou4f3; Vglut3; ampullary organs; beta-parvalbumins; developmental biology; electroreceptors; hair cells; hh; neuromasts; neuroscience; oncomodulin; otoferlin; stem cells; synaptic ribbons; voltage-gated ion channels