title: Melittin inhibits the invasion of MCF-7 cells by downregulating CD147 and MMP-9 expression.
abstract: Tumor invasion and metastasis are the critical steps in determining the aggressive phenotype of human cancers. Melittin, a major component of bee venom, has been reported to induce apoptosis in several cancer cells. However, the mechanisms of melittin involvement in cancer invasion and metastasis remain unclear. Our previous study indicated that melittin inhibits cyclophilin A (CypA), a ubiquitously distributed peptidylprolylcis-transisomerase, in macrophage cells. In the present study, the Transwell assay results showed that melittin may downregulate the invasion level of MCF-7 cells in a dose-dependent manner. Additionally, it was also found, using flow cytometry and reverse transcription-polymerase chain reaction, that melittin decreased the expression of cluster of differentiation (CD)147 and matrix metallopeptidase-9 (MMP-9), whereas CypA upregulated the expression of CD147 and MMP-9. Overall, the present study indicated that melittin decreased the invasion level of MCF-7 cells by downregulating CD147 and MMP-9 by inhibiting CypA expression. The results of the present study provide an evidence for melittin in anticancer therapy and mechanisms.
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Fengyu
authors.initials: F
authors.lastname: Li
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Tan
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Peng
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Jia
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Qingmiao
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Huo
authors.affiliation: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhao
keywords: CD147; MCF-7 cells; MMP-9; cyclophilin A; melittin