title: Surface Plasmon Resonance-Based Concentration Determination Assay: Label-Free and Antibody-Free Quantification of Morpholinos.
abstract: Surface plasmon resonance (SPR) is a physical process that allows label-free and real-time detection of biomolecular interactions. SPR provides a rapid and quantitative method for studying interactions of macromolecules such as proteins and nucleic acids. Antisense Morpholino oligomers are widely used to regulate gene expression and the US FDA has approved a Morpholino drug for treatment of Duchenne muscular dystrophy. Here, we describe an antibody-free, label-free, high-throughput, and walk-away SPR method for quantification of Morpholino compounds extracted from biological specimens. This provides a valuable way for determining pharmacokinetics and pharmacodynamics of Morpholino oligomers in biological matrices for research and therapeutic applications.
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR, 97331, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Boutilier
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR, 97331, USA. hong.moulton@oregonstate.edu.
authors.forename: Hong M
authors.initials: HM
authors.lastname: Moulton
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D060172:Morpholinos / Q000032:analysis* / Q000097:blood / Q000652:urine; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D060172:Morpholinos
keywords: Antibody-free; Bioavailability; Concentration analysis; Label-free; Morpholino oligomers; PMO; PPMO; SPR; Surface plasmon resonance