title: Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance.
abstract: A major conceptual breakthrough in cell signaling has been the finding of EV as new biomarker shuttles in body fluids. Now, one of the major challenges in using these nanometer-sized biological entities as diagnostic marker is the development of translational methodologies to profile them. SPR offers a promising label-free and real time platform with a high potential for biomarker detection. Therefore, we aimed to develop a uniform SPR methodology to detect specific surface markers on EV derived from patient with CHD. EVs having an approximate size range between 30 and 100 nm (~48.5%) and 100-300 nm (~51.5%) were successfully isolated. The biomarker profile of EV was verified using immunogold labeling, ELISA and SPR. Using SPR, we demonstrated an increased binding of EV derived from patients with CHD to anti-ICAM-1 antibodies as compared to EV from healthy donors. Our current findings open up novel opportunities for in-depth and label-free investigation of EV.
authors.affiliation: Hasselt University, Biomedical Research Institute, Hasselt, Belgium. Electronic address: Baharak.hosseinkhani@uhasselt.be.
authors.forename: Baharak
authors.initials: B
authors.lastname: Hosseinkhani
authors.affiliation: Maastricht University, Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht, The Netherlands.
authors.forename: Nynke
authors.initials: N
authors.lastname: van den Akker
authors.affiliation: Hasselt University, Institute for Materials Research, Hasselt, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: D'Haen
authors.affiliation: Maastricht University, Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht, The Netherlands.
authors.forename: Mick
authors.initials: M
authors.lastname: Gagliardi
authors.affiliation: Hasselt University, Biomedical Research Institute, Hasselt, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Struys
authors.affiliation: Hasselt University, Biomedical Research Institute, Hasselt, Belgium.
authors.forename: Ivo
authors.initials: I
authors.lastname: Lambrichts
authors.affiliation: Department of Cardiovascular Medicine, University Hospital Münster, Münster, Germany; Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Münster, Münster, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Waltenberger
authors.affiliation: Flemish Institute for Technological Research (VITO), Mol, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Nelissen
authors.affiliation: Hasselt University, Theoretical Physics, Hasselt, Belgium; Flemish Institute for Technological Research (VITO), Mol, Belgium.
authors.forename: Jef
authors.initials: J
authors.lastname: Hooyberghs
authors.affiliation: Maastricht University, Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht, The Netherlands.
authors.forename: Daniel G M
authors.initials: DGM
authors.lastname: Molin
authors.affiliation: Hasselt University, Biomedical Research Institute, Hasselt, Belgium. Electronic address: Luc.Michiels@uhasselt.be.
authors.forename: Luc
authors.initials: L
authors.lastname: Michiels
mesh_terms: D015415:Biomarkers; D003327:Coronary Disease; D042783:Endothelial Cells; D000067128:Extracellular Vesicles; D006801:Humans; D007249:Inflammation; D036103:Nanotechnology / Q000379:methods; D020349:Surface Plasmon Resonance
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Biosensor; Cardiovascular disease; Endothelial cells; Extracellular vesicles; Inflammation