title: Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression.
abstract: OBJECTIVE
  To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression.
  
  
  METHODS
  We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS®EasyQ®HPV). The samples were divided into three groups: positive (n=75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n=52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n=48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p=0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p<0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633; 95% CI, 1.399-4.954, p=0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups.
  
  
  CONCLUSIONS
  The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression.
authors.affiliation: Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Núcleo de Prevenção de Doenças Ginecológicas, São Paulo, SP, Brazil. Electronic address: acchuery@hotmail.com.
authors.forename: Ana Carolina Silva
authors.initials: ACS
authors.lastname: Chuery
authors.affiliation: Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brazil.
authors.forename: Ismael Dale Cotrim Guerreiro da
authors.initials: IDCGD
authors.lastname: Silva
authors.affiliation: Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Núcleo de Prevenção de Doenças Ginecológicas, São Paulo, SP, Brazil.
authors.forename: Julisa Chamorro Lascasas
authors.initials: JCL
authors.lastname: Ribalta
authors.affiliation: Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Núcleo de Prevenção de Doenças Ginecológicas, São Paulo, SP, Brazil.
authors.forename: Neila Maria de Góis
authors.initials: NMG
authors.lastname: Speck
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001120:Arginine / Q000235:genetics; D003062:Codon; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology*
chemical_list: D003062:Codon; C118860:E6 protein, Human papillomavirus type 6; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D001120:Arginine
keywords: Arginine; Genetic polymorphisms; Human papillomavirus; Oncoproteins; p53 gene