title: Fluorinated methacrylamide chitosan sequesters reactive oxygen species to relieve oxidative stress while delivering oxygen.
abstract: Antioxidants play an important role in regulating overabundant reactive oxygen species (ROS) in wound healing to reduce oxidative stress and inflammation. In this work, we demonstrate for the first time that functionalization of methacrylamide chitosan (MAC) with aliphatic pentadecafluoro chains, to synthesize pentadecafluoro-octanoyl methacrylamide chitosan (MACF), enhances the antioxidant capacity of the MAC base hydrogel material, while being able to deliver oxygen for future enhanced wound healing applications. As such, MACF was shown to sequester more nitric oxide (p < 0.01) and hydroxyl (p < 0.0001) radicals as compared to the negative control even when delivering additional oxygen. MACF's beneficial antioxidant capacity was further confirmed in in vitro cell culture experiments using human dermal fibroblasts stressed with 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH). © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2368-2374, 2017.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Akron, Ohio, 44325.
authors.forename: Pritam S
authors.initials: PS
authors.lastname: Patil
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Akron, Ohio, 44325.
authors.forename: Nic D
authors.initials: ND
authors.lastname: Leipzig
mesh_terms: D000178:Acrylamides / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005466:Fluorocarbons / Q000737:chemistry / Q000494:pharmacology*; D054879:Halogenation; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D000178:Acrylamides; D000975:Antioxidants; D005466:Fluorocarbons; D017382:Reactive Oxygen Species; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; C045985:methacrylamide; D010100:Oxygen
keywords: chitosan; oxidative stress; perfluorocarbons; reactive oxygen species