title: Effect of aquo-alchoholic extract of Glycyrrhiza glabra against Pseudomonas aeruginosa in Mice Lung Infection Model.
abstract: The prevalence of lung infection caused by Pseudomonas aeruginosa strains that are classified as multi-drug resistant has increased considerably and is mainly attributed to relative insufficiency of potent chemotherapeutic modalities. The present study was conducted to evaluate the antimicrobial activity of aquo-alcoholic extract of Glycyrrhiza glabra against the P. aeruginosa causing lung infection in Swiss albino mice. The study involves evaluation of lethal dose of P. aeruginosa in Swiss albino mice and analysis of disease manifestation that includes bacteremia, hypothermia, reduction in body weight and other parameters for 48h of infection. Physical manifestations of infected mice showed a significant decline in body temperature that is 29±0.57°C (at 48th h) from 38.81±0.33°C (0h) and 30% weight loss was observed at the end of the study. Further the efficacy of G. glabra extract against lung infection induced with the calculated lethal dose was evaluated by employing bacteremia, histopathology and radiological analysis. Bacterial burden showed that 2.30±0.02 Log10CFU/mL at day 7, a significant decline in the bacterial load as compared to day 1 when the bacterial burden was found to be 3.32±0.1 Log10CFU/mL. Histopathological results showed more diffuse and patchy accumulation of inflammatory cells within the alveolar space also the infiltrates were noted in all the lung section of infected mice. In treated animal group improved lung histology was seen with the exudates were less seen in D1 dose (20mg/kg) and disappeared in D2 dose (80mg/kg). The study clearly declares that the G. glabra extract is effective against lung infection caused by P. aeruginosa at dose of 80mg/kg. The LCMS results revealed that the extract contains Glycyrrhizin, Stigmasterol and Ergosterol, Licochalcone and Glabridin. The current study expected to further exploit the biomedical properties of this extract in the preparation of a potent regimen against such threatening pathogen.
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi - 110054, India.
authors.forename: Ankita Singh
authors.initials: AS
authors.lastname: Chakotiya
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi - 110054, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Tanwar
authors.affiliation: Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Delhi - 110054, India.
authors.forename: Pranay
authors.initials: P
authors.lastname: Srivastava
authors.affiliation: Department of Biotechnology, Jamia Hamdard, Delhi - 110062, India.
authors.forename: Alka
authors.initials: A
authors.lastname: Narula
authors.affiliation: Defence Food Research Laboratory, Mysuru - 570011, India. Electronic address: rksharmadrl@yahoo.com.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006035:Glycyrrhiza / Q000737:chemistry*; D007249:Inflammation / Q000188:drug therapy / Q000382:microbiology; D007529:Isoflavones / Q000494:pharmacology; D008168:Lung / Q000187:drug effects* / Q000382:microbiology*; D008297:Male; D051379:Mice; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
chemical_list: D007529:Isoflavones; D010636:Phenols; D010936:Plant Extracts; C107601:glabridin
keywords: Glycyrrhiza glabra; Liquid chromatography mass spectrometry; Lung infection; Pseudomonas aeruginosa