title: Evaluation of cellobiose dehydrogenase and laccase containing culture fluids ofTermitomycessp. OE147 for degradation of Reactive blue 21.
abstract: This study evaluates culture filtrate, rich in cellobiose dehydrogenase and laccases, ofTermitomycessp. OE 147, in decolouration and degradation of Reactive blue (RB) 21. About 35% decolouration was achieved at low volumes of the culture supernatant without addition of external redox mediators. An optimized dye to culture fluid ratio (75 ppm: 0.1 ml) at a pH of 4-5 resulted in removal of colour by 60%. The degradation products of RB21 were analysed by Electron Spray Ionization-Mass Spectrometry and several small molecules (ofm/z106-199) were detected. These were concluded to be o-Xylene, 2,3-Dihydro-1H-isoindole, Isoindole-1,3-dione, 2,Benzenesulfonyl-ethanol, (4-Hydroxy-phenyl)-sulfamic acid, 2,3-Dihydro-1H-isoindole-5-sulfonic acid and proposed to result from joint action of cellobiose dehydrogenase, laccase, peroxidases and unidentified oxidoreductases present in the culture fluids. Based on the products formed and the known reactions of these enzymes, a degradation pathway was proposed for RB21. The culture fluid was also effective in decolouration (by about 50%) and detoxification (by ∼25%) of the combined effluent collected from a local mill indicating a treatment process that bypasses use of H2O2and toxic mediators.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India; School of Biotechnology, Shri Mata Vaishno Devi University, Katra, Jammu and Kashmir 182320, India.
authors.forename: Rishabh
authors.initials: R
authors.lastname: Gangwar
authors.affiliation: School of Biotechnology, Shri Mata Vaishno Devi University, Katra, Jammu and Kashmir 182320, India.
authors.forename: Shafaq
authors.initials: S
authors.lastname: Rasool
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India.
authors.forename: Saroj
authors.initials: S
authors.lastname: Mishra
keywords: Cellobiose dehydrogenase/laccase; Complex phthalocyanine dyes; Fungal culture filtrates; Termitomyces sp. OE 147; Textile effluents