title: Inhibitory effects of 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid on lipopolysaccharide-induced TNF-α, IL-1β, and the high mobility group box 1 release in macrophages.
abstract: We investigated the anti-inflammatory effects of 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid (HLEDA)-a lupane-type triterpene isolated from leaves of Acanthopanax gracilistylus W. W.Smith (AGS), as well as the underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW264.7 cells. Our results demonstrated that HLEDA concentration-dependently reduced the production of nitric oxide (NO), signiﬁcantly suppressed LPS-induced expression of TNF-α and IL-1β at the mRNA and protein levels in RAW264.7 cells. Further analysis revealed that HLEDA could reduce the secretion of High Mobility Group Box 1 (HMGB1). Additionally, the results showed that HLEDA efficiently decreased nuclear factor-kappaB (NF-κB) activation by inhibiting the degradation and phosphorylation of IκBα. These results suggest that HLEDA exerts anti-inflammatory properties in LPS-induced macrophages, possibly through inhibition of the NF-κB signaling pathway, which mediates the expression of pro-inflammatory cytokines. These results warrant further studies that would concern candidate therapy for diseases, such as fulminant hepatitis and rheumatology of triterpenoids in AGS.
authors.affiliation: a School of Pharmacy, Hunan University of Chinese Medicine , Changsha , China.|b School of Chemistry and Chemical Engineering, Central South University , Changsha , China.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: b School of Chemistry and Chemical Engineering, Central South University , Changsha , China.|c Changsha Broad-Ocean Bio-science and Technique Co.,Ltd. , Changsha , China.
authors.forename: Qin-Peng
authors.initials: QP
authors.lastname: Zou
authors.affiliation: d Department of Clinical Biochemistry , Xiangya Medical College, Central South University , Changsha , China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Huang
authors.affiliation: e College of Pharmacy, KyungHee University , Seoul , Korea.
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Yook
authors.affiliation: f College of Pharmacy, Chung-Ang University , Seoul , Korea.
authors.forename: Wan-Kyunn
authors.initials: WK
authors.lastname: Whang
authors.affiliation: g Korea Research Institute of Bioscience and Biotechnology , Chungbuk , Korea.
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.affiliation: g Korea Research Institute of Bioscience and Biotechnology , Chungbuk , Korea.
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D028001:Eleutherococcus; D015870:Gene Expression; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D007249:Inflammation; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D010766:Phosphorylation / Q000187:drug effects; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; C068052:3-hydroxylup-20(29)en-23,28-dioic acid; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; C400150:Acanthopanax gracilistylus, extract
keywords: 3α-hydroxy-lup-20(29)-en-23,28-dioic acid; HMGB1; IL-1β; TNF-α; anti-inflammatory