title: Evolutionary Transcriptomics and Proteomics: Insight into Plant Adaptation.
abstract: Comparative transcriptomics and proteomics (T&P) have brought biological insight into development, gene function, and physiological stress responses. However, RNA-seq and high-throughput proteomics remain underutilised in studies of plant adaptation. These methodologies have created discovery tools with the potential to significantly advance our understanding of adaptive diversification. We outline experimental recommendations for their application. We discuss analysis models and approaches that accelerate the identification of adaptive gene sets and integrate transcriptome, proteome, phenotypic, and environmental data. Finally, we encourage widespread uptake and future developments in T&P that will advance our understanding of evolution and adaptation.
authors.affiliation: Max Planck Institute for Chemical Ecology, Jena, Germany. Electronic address: cvoelckel@ice.mpg.de.
authors.forename: Claudia
authors.initials: C
authors.lastname: Voelckel
authors.affiliation: Faculty of Biology, Health, and Medicine, University of Manchester, Manchester, UK.
authors.forename: Nicole
authors.initials: N
authors.lastname: Gruenheit
authors.affiliation: Institute for Fundamental Sciences, Massey University, Palmerston North, New Zealand.
authors.forename: Peter
authors.initials: P
authors.lastname: Lockhart
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics / Q000502:physiology*
chemical_list: D020543:Proteome
keywords: RNA-seq; adaptive diversification; ecology; evolution; gene expression modelling; shotgun proteomics