title: Isolation, Culture, and Expansion of Mesenchymal Stem Cells.
abstract: Mesenchymal stem cells (MSCs), together with hematopoietic stem cells (HSCs), are the most frequently used cell type for cell-based therapeutics. As for other cell types intended for research and translational use, it is important to establish correctly typed cell lines from human tissue donations. Here, we describe methods for isolating, culturing, and identifying MSCs from various tissues obtained through human tissue donation. The methods have been used in the context of a biobank, prepared as standard operating procedures (SOPs), ensuring traceability and reproducibility of cell production.
authors.affiliation: StemTek Therapeutics, Kabi612, Parque Tecnologico de Bizkaia, Astondo Bidea s/n, Derio, 48160, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: Ferrin
authors.affiliation: StemTek Therapeutics, Kabi612, Parque Tecnologico de Bizkaia, Astondo Bidea s/n, Derio, 48160, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: Beloqui
authors.affiliation: StemTek Therapeutics, Kabi612, Parque Tecnologico de Bizkaia, Astondo Bidea s/n, Derio, 48160, Spain.
authors.forename: Lorea
authors.initials: L
authors.lastname: Zabaleta
authors.affiliation: StemTek Therapeutics, Kabi612, Parque Tecnologico de Bizkaia, Astondo Bidea s/n, Derio, 48160, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Salcedo
authors.affiliation: StemTek Therapeutics, Kabi612, Parque Tecnologico de Bizkaia, Astondo Bidea s/n, Derio, 48160, Spain.
authors.forename: Cesar
authors.initials: C
authors.lastname: Trigueros
authors.affiliation: StemTek Therapeutics, Kabi612, Parque Tecnologico de Bizkaia, Astondo Bidea s/n, Derio, 48160, Spain. agmartin@stemtektherapeutics.com.
authors.forename: Angel G
authors.initials: AG
authors.lastname: Martin
mesh_terms: D018070:Biological Specimen Banks; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D015203:Reproducibility of Results
keywords: Bone marrow; Dental pulp; Lipoaspirate; Mesenchymal stem cells; Umbilical cord