title: Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant speciesin vitro.
abstract: This article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematicin vitroassessment of responses of roGFP2-based probes to physiologically relevant oxidant species" [1]. The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO-), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article [1].
authors.affiliation: Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Müller
authors.affiliation: Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Jannis F
authors.initials: JF
authors.lastname: Schneider
authors.affiliation: Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Adriana
authors.initials: A
authors.lastname: Degrossoli
authors.affiliation: Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Lupilova
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
authors.forename: Tobias P
authors.initials: TP
authors.lastname: Dick
authors.affiliation: Institute of Biochemistry and Pathobiochemistry - Microbial Biochemistry, Ruhr-University Bochum, 44780 Bochum, Germany.
authors.forename: Lars I
authors.initials: LI
authors.lastname: Leichert
keywords: Genetically encoded redox probes; H2O2; HOCl; Nitric oxide; Peroxynitrite; Polysulfides; glutathione