title: Augmented lipid accumulation in ethyl methyl sulphonate mutants of oleaginous microalga for biodiesel production.
abstract: The aim of this work was to generate high lipid accumulating mutants of Chlorella minutissima (CM) using ethyl methyl sulphonate (EMS) as a random chemical mutagen. Amid the 5% surviving cells after exposure to EMS (2M), three fast growing mutants (CM2, CM5, CM7) were selected and compared with wild type for lipid productivity and biochemical composition. Among these mutants, CM7 showed the maximum biomass (2.4g/L) and lipid content (42%) as compared to wild type (1.5g/L; 27%). Further, the mutant showed high photosynthetic pigments with low starch content signifying the re-allocation of carbon flux to lipid. The obtained mutant showed no visible morphological changes in comparison to its WT. The fatty acid profile showed increase in monounsaturated fatty acids while decreased saturated and polyunsaturated fatty acids signifying good quality biodiesel. The mutant strain thus obtained can be optimized further and applied for enhanced biodiesel production.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, India.
authors.forename: Juhi
authors.initials: J
authors.lastname: Mehtani
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Arora
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Patel
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Jain
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, India.
authors.forename: Parul A
authors.initials: PA
authors.lastname: Pruthi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, India.
authors.forename: Kirshna Mohan
authors.initials: KM
authors.lastname: Poluri
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, India. Electronic address: vikasfbs@iitr.ac.in.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
mesh_terms: D056804:Biofuels; D018533:Biomass; D002708:Chlorella; D005227:Fatty Acids; D008055:Lipids; D058086:Microalgae
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids
keywords: Chlorella minutissima; Ethyl Methyl Sulphonate; Lipid content; Mutant; Triacylglycerol