title: Artemisinin loaded chitosan magnetic nanoparticles for the efficient targeting to the breast cancer.
abstract: Artemisinin, a natural anti-malarial agent, also possesses anti-proliferative and anti-angiogenic activity in cancer cells with very low toxicity to normal healthy cells. Drug loaded magnetic nanoparticles by using external magnetic field could selectively accumulate the drug at the target site and thereby reduce the doses required to achieve therapeutic concentration which may otherwise produce serious side effects on healthy cells. In the present study the artemisinin magnetic nanoparticles were successfully formulated using chitosan by ionic-gelation method. The developed magnetic nanoparticles of artemisinin were smooth and spherical in nature and their size was in the range of 349-445nm. The polydispersity index (PDI) and zeta potential of the formulated nanoparticles were in the range of 0.373-0.908 and -9.34 to -33.3 respectively. They showed 55% to 62.5% of drug encapsulation efficiency and 20% to 25% drug loading capacity. Around 62% to 78% of artemisinin was released from the artemisinin magnetic nanoparticles over the period of 48h. On application of physiologically acceptable external magnetic field, FITC conjugated artemisinin magnetic nanoparticles showed an enhanced accumulation of nanoparticles in the 4T1 breast tumour tissues of BALB/c mice model.
authors.affiliation: Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu, India. Electronic address: natesansubbu@gmail.com.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Natesan
authors.affiliation: Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Chandrasekar
authors.initials: C
authors.lastname: Ponnusamy
authors.affiliation: Department of Pharmaceutics, SRM University, SRM Nagar, Kattankulathur, Tamil Nadu, India.
authors.forename: Abimanyu
authors.initials: A
authors.lastname: Sugumaran
authors.affiliation: Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: Chelladurai
authors.affiliation: Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Sharavanan
authors.initials: S
authors.lastname: Shanmugam Palaniappan
authors.affiliation: Department of Biotechnology, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu, India.
authors.forename: Rajaguru
authors.initials: R
authors.lastname: Palanichamy
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry*; D037621:Artemisinins / Q000008:administration & dosage* / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004195:Disease Models, Animal; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D005260:Female; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D037621:Artemisinins; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D048271:Chitosan
keywords: Artemisinin; Breast cancer; Magnetic nanoparticles; Targeting potential