title: Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae.
abstract: In this study, we present the characterization and genomic data of three Achromobacter phages belonging to the family Siphoviridae. Phages 83-24, JWX and JWF were isolated from sewage samples in Paris and Braunschweig, respectively, and infect Achromobacter xylosoxidans, an emerging nosocomial pathogen in cystic fibrosis patients. Analysis of morphology and growth parameters revealed that phages 83-24 and JWX have similar properties, both have nearly the same head and tail measurements, and both have a burst size between 85 and 100 pfu/cell. In regard to morphological properties, JWF had a much longer and more flexible tail compared to other phages. The linear double-stranded DNAs of all three phages are terminally redundant and not circularly permutated. The complete nucleotide sequences consist of 81,541 bp for JWF, 49,714 bp for JWX and 48,216 bp for 83-24. Analysis of the genome sequences showed again that phages JWX and 83-24 are quite similar. Comparison to the GenBank database via BLASTN revealed partial similarities to Roseobacter phage RDJL phi1 and Burkholderia phage BcepGomr. In contrast, BLASTN analysis of the genome sequence of phage JWF revealed only few similarities to non-annotated prophage regions in different strains of Burkholderia and Mesorhizobium.
authors.affiliation: Department of Microbiology/Genetechnology, University of Bielefeld, 33615, Bielefeld, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Dreiseikelmann
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Brunswick, Germany.
authors.forename: Boyke
authors.initials: B
authors.lastname: Bunk
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Brunswick, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Central Facility for Microscopy, Helmholtz Centre for Infection Research, 38124, Brunswick, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Protein Analytics Platform, Helmholtz Centre for Infection Research, 38124, Brunswick, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124, Brunswick, Germany. johannes.wittmann@dsmz.de.
authors.forename: Johannes
authors.identifier: http://orcid.org/0000-0002-7275-9927
authors.initials: J
authors.lastname: Wittmann
mesh_terms: D042402:Achromobacter / Q000821:virology*; D001483:Base Sequence; D002874:Chromosome Mapping; D016679:Genome, Viral; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification