title: Respirometry increases cortisol levels in rainbow trout Oncorhynchus mykiss: implications for measurements of metabolic rate.
abstract: This study aimed to assess the extent to which chasing, handling and confining Oncorhynchus mykiss to a small respirometer chamber during respirometric experiments is stressful and affects metabolic measurements. The study observed increased cortisol levels in animals tested using a chase protocol and subsequent intermittent-flow respirometry, suggesting that this procedural treatment may stress animals.
authors.affiliation: University of Manitoba, Department of Biological Sciences, 50 Sifton Road, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Murray
authors.affiliation: University of Manitoba, Department of Biological Sciences, 50 Sifton Road, Winnipeg, MB, R3T 2N2, Canada.|Lakehead University, Department of Biology, 955 Oliver Road, Thunder Bay, ON, P7B 5E, Canada.|IISD-Experimental Lakes Area, 111 Lombard Avenue, Suite 325, Winnipeg, MB, R3B OT4, Canada.
authors.forename: M D
authors.initials: MD
authors.lastname: Rennie
authors.affiliation: Technical University of Denmark, Jaegersborg Alle 1, 2920, Charlottenlund, Denmark.
authors.forename: J C
authors.initials: JC
authors.lastname: Svendsen
authors.affiliation: Fisheries & Oceans Canada, Freshwater Institute, 501 University Crescent, Winnipeg, MB, R3T 2N6, Canada.
authors.forename: E C
authors.initials: EC
authors.lastname: Enders
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000097:blood / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D006854:Hydrocortisone
keywords: fishes; metabolism; salmonid; standard metabolic rate; static respirometry; stress