title: Evaluation of the effectiveness of micro-Raman spectroscopy in monitoring the mineral contents change of human enamel in vitro.
abstract: The purpose of this in vitro study was to investigate the efficacy of micro-Raman spectroscopy on detecting mineral content change during the demineralization and de/remineralization cycling process. The enamel samples (n = 55) were randomly divided into three groups and separately treated with demineralization solution (n = 20), de/remineralization cycling solution (n = 30), and distilled water (n = 5). Micro-Raman spectroscopy, microhardness (MHS), and the released calcium ions concentration were performed before and after treatment, respectively. A one-way analysis of variance (ANOVA) with a post hoc Tukey test was used to analyze the results. The Spearman correlation coefficients among the parameters of Raman relative intensity decrease (RRID%), the percentage of MHS loss (PML), and the released calcium ions concentration were also analyzed. In demineralization group, RRID%, PML, and released calcium ions concentration were highly correlated with each other (r = 0.979, p < 0.001; r = 0.984, p < 0.001; and r = 0.983, p < 0.001, respectively). While for the de/remineralization cycling group, there also existed a high correlation between RRID% and PML (r = 0.987, p < 0.001). In conclusion, micro-Raman spectroscopy could effectively monitor the mineral content change, and its efficacy was validated by the measurement of released calcium ions concentration and MHS.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China.|Department of Prosthodontics, Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sa
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Feng
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Lei
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China.|Tianjin Stomatological Hospital, Nankai University, 75 Dagu Road, Heping District, Tianjin, 300000, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China. jiangtao2006@whu.edu.cn.|Department of Prosthodontics, Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China. jiangtao2006@whu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China. wang.yn@whu.edu.cn.|Department of Prosthodontics, Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People's Republic of China. wang.yn@whu.edu.cn.
authors.forename: Yining
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002118:Calcium / Q000032:analysis; D003743:Dental Enamel / Q000737:chemistry*; D006244:Hardness; D006801:Humans; D007477:Ions; D008903:Minerals / Q000032:analysis*; D055100:Optical Fibers; D013059:Spectrum Analysis, Raman / Q000379:methods*; D017001:Tooth Demineralization; D014089:Tooth Remineralization
chemical_list: D007477:Ions; D008903:Minerals; D002118:Calcium
keywords: Demineralization; Enamel; Micro-Raman spectroscopy; Mineral content; Remineralization