title: Fluorescence Image Analyzer - FLIMA: software for quantitative analysis of fluorescence in situ hybridization.
abstract: The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed.
authors.affiliation: Laboratório de Evolução Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brasil hallana19@gmail.com.|Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil hallana19@gmail.com.
authors.forename: H C M
authors.initials: HC
authors.lastname: Silva
authors.affiliation: Empresa Fermento, Manaus, AM, Brasil.
authors.forename: M M C
authors.initials: MM
authors.lastname: Martins-Júnior
authors.affiliation: Centro de Ciências da Saúde, Coordenação de Medicina, Universidade Federal de Roraima, Boa Vista, RR, Brasil.
authors.forename: L B
authors.initials: LB
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Evolução Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brasil.|Laboratório de Citogenômica Animal, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brasil.
authors.forename: D A
authors.initials: DA
authors.lastname: Matoso
mesh_terms: D002875:Chromosomes; D005069:Evaluation Studies as Topic; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012984:Software