title: Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation.
abstract: Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (-47%, P = .01), aromatase gene expression (-36%, P = .02), and estradiol production (-49%, P = .01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (-18%, P < .01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.
authors.affiliation: 1 Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Fayezi
authors.affiliation: 1 Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Marefat
authors.initials: M
authors.lastname: Ghaffari Novin
authors.affiliation: 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Darabi
authors.affiliation: 1 Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Norouzian
authors.affiliation: 3 Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nouri
authors.affiliation: 4 Women's Reproductive Health Research Center, Women University Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Laya
authors.initials: L
authors.lastname: Farzadi
authors.affiliation: 5 UMR INSERM-UPMC 1166 ICAN, Pavillon Benjamin Delessert, Hôpital de la Pitié, Paris, France.
authors.forename: Maryam
authors.initials: M
authors.lastname: Darabi
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis; D001141:Aromatase / Q000378:metabolism; D002470:Cell Survival; D018920:Coculture Techniques; D054885:Cumulus Cells / Q000187:drug effects / Q000201:enzymology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D019301:Oleic Acid / Q000008:administration & dosage; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D013256:Steroids; D014280:Triglycerides; D019301:Oleic Acid; D004958:Estradiol; D001141:Aromatase; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase
keywords: germinal vesicle; lipid; paracrine; steroid hormone