title: Mediator lipidomics by liquid chromatography-tandem mass spectrometry.
abstract: Comprehensive quantitative analysis of lipid mediators using liquid chromatography-tandem mass spectrometry is an effective strategy in the elucidation of disease mechanisms; but technically, it has been and is still a great challenge to achieve reliable datasets that cover variety of lipid metabolites contained at trace levels in complex biological matrices. In this opinion article, we introduce our experiences in developing lipid mediator profiling systems, and deliver some comments on limitations of current methodology.
authors.affiliation: Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Life Sciences Core Facility, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: kita@m.u-tokyo.ac.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kita
authors.affiliation: Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Suzumi M
authors.initials: SM
authors.lastname: Tokuoka
authors.affiliation: Department of Lipidomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Lipid Signaling, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D008055:Lipids
keywords: Cross-platform; Eicosanoids; Mass spectrometer; Quantification