title: Genomic Evidence for Bacterial Determinants Influencing Obesity Development.
abstract: Obesity is a major global public health problem requiring multifaceted interventional approaches including dietary interventions with probiotic bacteria. High-throughput genome sequencing of microbial communities in the mammalian gastrointestinal system continues to present diverse protein function information to understand the bacterial determinants that influence obesity development. The goal of the research reported in this article was to identify biological processes in probiotic bacteria that could influence the mechanisms for the extraction of energy from diet in the human gastrointestinal system. Our research strategy of combining bioinformatics and visual analytics methods was based on the identification of operon gene arrangements in genomes ofLactobacillusspecies andAkkermansiamuciniphilathat include at least a gene for a universal stress protein. The two major findings from this research study are related toLactobacillus plantarumandAkkermansia muciniphilabacteria species which are associated with weight-loss. The first finding is thatLactobacillus plantarumstrains have a two-gene operon that encodes a universal stress protein for stress response and the membrane translocator protein (TSPO), known to function in mitochondrial fatty acid oxidation in humans. The second finding is the presence of a three-gene operon inAkkermansia muciniphilathat includes a gene whose human mitochondrial homolog is associated with waist-hip ratio and fat distribution. From a public health perspective, elucidation of the bacterial determinants influencing obesity will help in educating the public on optimal probiotic use for anti-obesity effects.
authors.affiliation: College of Science, Engineering and Mathematics, Bethune-Cookman University, Daytona Beach, FL 32114, USA. isokpehir@cookman.edu.
authors.forename: Raphael D
authors.initials: RD
authors.lastname: Isokpehi
authors.affiliation: Division of Arts and Sciences, Jarvis Christian College, Hawkins, TX 75765, USA. shaneka.s.simmons@gmail.com.|Center of Excellence in Minority Health and Health Disparities, Jackson State University, Jackson, MS 39217, USA. shaneka.s.simmons@gmail.com.
authors.forename: Shaneka S
authors.initials: SS
authors.lastname: Simmons
authors.affiliation: Petrock College of Health Sciences, Bethune-Cookman University, Daytona Beach, FL 32114, USA. johnsonma@cookman.edu.
authors.forename: Matilda O
authors.initials: MO
authors.lastname: Johnson
authors.affiliation: Center of Excellence in Minority Health and Health Disparities, Jackson State University, Jackson, MS 39217, USA. marinelle.payton@jsums.edu.
authors.forename: Marinelle
authors.initials: M
authors.lastname: Payton
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004032:Diet; D041981:Gastrointestinal Tract; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D023281:Genomics; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007778:Lactobacillus / Q000235:genetics* / Q000502:physiology; D009765:Obesity / Q000382:microbiology*; D009876:Operon; D019936:Probiotics / Q000627:therapeutic use; D061327:Verrucomicrobia / Q000235:genetics* / Q000502:physiology; D049629:Waist-Hip Ratio
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins
keywords: Akkermansia; Lactobacillus; bacteria; body weight; microbiome; obesity; overweight; probiotics; public health; universal stress proteins