title: A high-fat diet impairs reproduction by decreasing the IL1β level in mice treated at immature stage.
abstract: Obesity causes low-grade inflammation that is involved in male infertility. Interleukin 1 beta (IL1β) plays an important role in this process. A high-fat diet (HFD) is the most common cause of obesity. However, the effect of a HFD on IL1β and its consequence in reproduction remain unclear. We established a HFD model in mice treated at immature stage (mice-TIS) and mice treated at mature stage (mice-TMS). Surprisingly, we found that a HFD decreased IL1β levels and was accompanied by an increase in testosterone in mice-TIS, while the reverse results were observed in mice-TMS. In addition, a HFD caused a reduction in testis macrophages and in the expression of inflammasome-related genes and proteins in mice-TIS. Furthermore, we found that IL1β inhibited testosterone secretion through down-regulating the gene expression of P450SCC and P450c17. However, the influence on mice-TIS that were induced by a HFD was recovered by stopping the HFD. In this study, we are the first to report that a HFD impairs the reproductive system by decreasing IL1β and enhancing testosterone levels in mice-TIS, which are different from the effects in mice-TMS. This provides new ideas for the treatment of obesity-induced infertility.
authors.affiliation: Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Guizen
authors.initials: G
authors.lastname: Huang
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ao
authors.affiliation: Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Haoze
authors.initials: H
authors.lastname: Tan
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
authors.forename: Di
authors.initials: D
authors.lastname: Gong
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Kang
authors.affiliation: Department of Pediatric Surgery, Provincial People's Hospital, Guizhou, China.
authors.forename: Nini
authors.initials: N
authors.lastname: An
authors.affiliation: Department of Pediatric Surgery, Provincial People's Hospital, Guizhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, China. linping@scu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China. lugang992001@aliyun.com.
authors.forename: Lugang
authors.initials: L
authors.lastname: Huang
mesh_terms: D000367:Age Factors; D000818:Animals; D017209:Apoptosis; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D005854:Germ Cells / Q000378:metabolism; D007150:Immunohistochemistry; D058847:Inflammasomes / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D012098:Reproduction / Q000235:genetics / Q000276:immunology; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000378:metabolism
chemical_list: D058847:Inflammasomes; D053583:Interleukin-1beta; D013739:Testosterone; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme