title: Emergence of a deviating genotype VI pigeon paramyxovirus type-1 isolated from India.
abstract: Pigeon paramyxovirus type 1 (PPMV-1) is an antigenic variant of avian paramyxovirus type 1 (APMV-1), which infects pigeons. The virus causes high morbidity and mortality, creating an alarming state for the poultry industry. The present work describes the molecular and pathogenic characterization of a PPMV-1 strain isolated from pigeon in Bhopal, India. Complete genome sequence analysis revealed a genome of 15,192 nucleotides encoding six genes organized in the order 3'-N-P-M-F-HN-L-5'. The fusion gene sequence analysis showed the presence of multiple basic amino acids112R-R-Q-K-R-F117at the cleavage site corresponding to pathogenic strains. The mean death time and intracerebral pathogenicity index values indicated a mesogenic nature for the PPMV-1 isolate. On phylogenetic analysis, the strain clustered with genotype VI viruses, including isolates from pigeon and dove. The Bhopal strain showed significant intra and inter-genotype evolutionary distance, suggesting the emergence of a new sub-genotype, VIj.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
authors.forename: Ketan
authors.initials: K
authors.lastname: Ganar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
authors.forename: Moushumee
authors.initials: M
authors.lastname: Das
authors.affiliation: National Institute of High Security Animal Diseases, OIE reference Lab on Avian Influenza, Bhopal, MP, 462022, India.
authors.forename: Ashwin Ashok
authors.initials: AA
authors.lastname: Raut
authors.affiliation: National Institute of High Security Animal Diseases, OIE reference Lab on Avian Influenza, Bhopal, MP, 462022, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India. sachinku@iitg.ernet.in.
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D010856:Columbidae / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D007194:India; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins