title: Trametes robiniophilamay induce apoptosis and inhibit MMPs expression in the human gastric carcinoma cell line MKN-45.
abstract: Gastric carcinoma (GC) is one of the most common malignant tumors and is mainly treated by invasive surgeries. The present study aimed to investigate the treatment potential ofTrametes robiniophilaon GC using the human GC cell line MKN-45. Cells were incubated withTrametes robiniophilaat a concentration of 0, 5 and 10 mg/ml for 24 h. The apoptosis of the cell line was examined with acridine orange/ethidium bromide staining and flow cytometry. The expression of B-cell lymphoma (Bcl)-2, Fas, caspase-3, matrix metalloproteinase (MMP)-2 and MMP-9 was analyzed using reverse transcription-polymerase chain reaction and western blotting. With increasing drug concentrations, the proportion of apoptotic and necrotic cells increased. For a certain concentration, the apoptotic ratio also increased with increasing response times. Compared with the control group, theBcl-2,MMP-2andMMP-9expression levels in the MKN-45 cell line decreased, while the expression levels ofFasandcaspase-3increased (P<0.05), and the expression patterns were strengthened with increasing drug concentrations. The present study revealed thatTrametes robiniophilahad treatment potential on GC, and it may act on gastric cells through apoptotic induction and MMPs expression inhibition. Based on the present results,Trametes robiniophilamay be considered as an alternative approach for noninvasive therapy of GC. However, future studies should be performed to clarify this further.
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Xuening
authors.initials: X
authors.lastname: Ji
authors.affiliation: Department of Oncology, The Third People's Hospital of Dalian, Dalian, Liaoning 116033, P.R. China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Xiulian
authors.initials: X
authors.lastname: Quan
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
keywords: Trametes robiniophila; antitumor; apoptosis; gastric carcinoma; matrix metalloproteinases