title: Dual-mode electrochemical analysis of microRNA-21 using gold nanoparticle-decorated MoS2nanosheet.
abstract: The detection of microRNA plays an important role in early cancer diagnosis. Herein, a dual-mode electronic biosensor was developed for microRNA-21 (miRNA-21) detection based on gold nanoparticle-decorated MoS2nanosheet (AuNPs@MoS2). A classical DNA "sandwich" structure was employed to construct MoS2-based electrochemical sensor, including capture DNA, target miRNA-21 and DNA-modified nanoprobe. [Fe(CN)6]3-/4-and [Ru(NH3)6]3+were selected as electrochemical indicators to monitor the preparation process and evaluate the performance of MoS2-based electrochemical biosensor by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), respectively. Such MoS2-based biosensor exhibited excellent performance for miRNA-21 detection in the range from 10 fM to 1nM with detection limit of 0.78fM and 0.45fM for DPV and EIS technique, respectively. Furthermore, the proposed MoS2-based biosensor displayed high selectivity and stability, which could be used to determine miRNA-21 in human serum samples with satisfactory results. All data suggested that such MoS2-based nanocomposite may be a potential candidate for biosensing ranging from nucleic acid to protein detection.
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cao
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Zaiwei
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.affiliation: College of Geography and Biological Information, Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
authors.forename: Lixing
authors.initials: L
authors.lastname: Weng
authors.affiliation: Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China; Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. Electronic address: fanchunhai@sinap.ac.cn.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), Jiangsu National Syngerstic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts &Telecommunications, 9 Wenyuan Road, Nanjing 210023, China. Electronic address: iamlhwang@njupt.edu.cn.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D008982:Molybdenum / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry
chemical_list: D004220:Disulfides; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D006046:Gold; D008982:Molybdenum; D004247:DNA; C082964:molybdenum disulfide
keywords: Biosensor; Dual-mode; Gold nanoparticles; MicroRNA; Molybdenum disulfide