title: Polypropylene microtitre plates modified with [Cr(OH)6]3-for enhanced ELISA sensitivity.
abstract: Chromium solutions have been used as wet chemical modifiers for polymer microtitre plates used in improving immunoassay performance. However, polypropylene has been excluded from the list of potentially modifiable substrates (AnteoTechnologies, 2015). Here we show that untreated polypropylene microtitre plates can indeed be modified using a [Cr(OH)6]3-complex. Compared to unmodified polypropylene, the chromium modified surfaces demonstrate an up to 4-fold improvement in both assay sensitivity and signal intensity in an antigen capture ELISA. Atomic force microscope (AFM) images indicate that the chromium complex facilitates dispersion of the antibody, reducing aggregation.
authors.affiliation: Centre for Materials and Surface Science, Department of Chemistry and Physics, La Trobe University, VIC 3086, Australia; CSIRO, Clayton, VIC 3168, Australia.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Welch
authors.affiliation: CSIRO, Clayton, VIC 3168, Australia; Chimie ParisTech, Paris 75005, France.
authors.forename: Cedric J
authors.initials: CJ
authors.lastname: Lebot
authors.affiliation: CSIRO, Clayton, VIC 3168, Australia.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Easton
authors.affiliation: CSIRO, Clayton, VIC 3168, Australia.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Scoble
authors.affiliation: Centre for Materials and Surface Science, Department of Chemistry and Physics, La Trobe University, VIC 3086, Australia.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Pigram
authors.affiliation: CSIRO, Clayton, VIC 3168, Australia. Electronic address: Ben.Muir@csiro.au.
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Muir
mesh_terms: D000906:Antibodies; D002857:Chromium / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D056951:Photoelectron Spectroscopy; D011126:Polypropylenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D000906:Antibodies; D011126:Polypropylenes; D002857:Chromium