title: [Effects of bisphenol A on the expression of N-cadherin, Vimentin and FSHR in rat Sertoli cells].
abstract: Objective:To explore the effects of BPA on the expression of N-cadherin, Vimentin and FSHR in rat Sertoli cells.Methods:Primary Sertoli cells collected from prepuberty rats (18-21 d) were cultured for 48 h, and then they were treated with 0, 30, 50, 70 μmol/L BPA respectively for 24 h. The methods of MTT, real-time quantitative PCR and Western blotting were utilized to measure the cell ability of Sertoli cells, the mRNA and protein expression levels of N-cadherin, Vimentin and FSHR respectively.Results:Compared with control, the cell abilities of Sertoli cells in 50 μmol/L BPA group and 70 μmol/L BPA group increased significantly (P<0.05) . The cell abilities of Sertoli cells decreased with the increases of exposure doses of BPA. Compared with control, the expression of N-cadherin mRNA only increased in 30 μmol/L BPA group (P<0.05) , the expression of Vimentin mRNA decreased significantly in all doses group of BPA (P<0.05) , the expression of FSHR mRNA increased in all doses group of BPA (P<0.05) . Compared with the control, the protein levels of N-cadherin increased significantly in 50 μmol/L BPA group (P<0.05) , the protein levels of Vimentin decreased significantly in all doses group of BPA (P<0.05) , the protein levels of FSHR decreased significantly in 50 μmol/L BPA group and 70 μmol/L BPA group (P<0.05) .Conclusion:The mechanism of testicular toxicity from BPA might be the alterations of N-cadherin, Vimentin and FSHR by disturbing normal spermatogenesis.
authors.affiliation: MOE Key Laboratory of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Duan
authors.forename: S
authors.initials: S
authors.lastname: Tang
authors.forename: W T
authors.initials: WT
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Quan
authors.forename: S Q
authors.initials: SQ
authors.lastname: Qi
authors.forename: K D
authors.initials: KD
authors.lastname: Yang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015820:Cadherins; D010636:Phenols; D014746:Vimentin; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; FSHR; N-cadherin; Sertoli cells; Vimentin