title: Leukocyte Adhesion Under Hemodynamic Flow Conditions.
abstract: Vascular endothelial cells (ECs) line the luminal side of all blood vessels and act as a selective barrier between blood and tissue. ECs are constantly exposed to biochemical and biomechanical stimuli from the blood and underlying tissue. Fluid shear stress acts in parallel to the vessel wall, resulting from friction of blood against EC. Despite the importance of flow on normal EC function, much of the information regarding EC function and dysfunction has been derived from cells harvested, grown, and studied in static culture.In order to study the effects of shear stress on EC function a number of in vitro models have been developed. This manuscript provides methodology for use of a system which enables recirculation of leukocytes and cell culture medium over the endothelium for a period of several minutes to days and enables investigation of the effects of prolonged leukocyte coculture on both the endothelial and leukocyte populations.
authors.affiliation: Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK. chlawson@rvc.ac.uk.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lawson
authors.affiliation: Harefield Hospital, Imperial College, London, UB9 6JH, UK.
authors.forename: Marlene
authors.initials: M
authors.lastname: Rose
authors.affiliation: Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.
authors.forename: Sabine
authors.initials: S
authors.lastname: Wolf
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells; D004730:Endothelium, Vascular / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D013314:Stress, Mechanical
keywords: Endothelium; Leukocyte; Parallel-plate flow chamber; Shear stress