title: Detection of HIV RNA in dried blood spots and oral fluids.
abstract: : We evaluated detection of HIV-1 RNA from dried blood spots (DBS) and oral fluid specimens. Between February 2010 and August 2014, HIV-1 was newly diagnosed in eight (2.6%) study participants who had median blood HIV-1 RNA of 61 500 copies/ml (interquartile range 7500-146 000). RNA was detected in seven (87.5%) DBS and three (37.5%) oral fluid swabs but was not detected in either specimen from one participant. DBS may be a reasonable specimen collection method to detect acute infection.
authors.affiliation: aDepartment of Medicine bDepartment of Epidemiology cLaboratory Medicine, University of Washington, Seattle, Washington, USA.
authors.forename: Joanne D
authors.initials: JD
authors.lastname: Stekler
authors.forename: George
authors.initials: G
authors.lastname: Ure
authors.forename: Joan
authors.initials: J
authors.lastname: Dragavon
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.forename: Robert W
authors.initials: RW
authors.lastname: Coombs
mesh_terms: D000328:Adult; D001769:Blood / Q000821:virology*; D003430:Cross-Sectional Studies; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012367:RNA, Viral / Q000032:analysis*; D012463:Saliva / Q000821:virology*; D055815:Young Adult
chemical_list: D012367:RNA, Viral