title: Identification of Filamin-A and -B as potential biomarkers for prostate cancer.
abstract: OBJECTIVE
  A novel strategy for prostate cancer (PrCa) biomarker discovery is described.
  
  
  METHODS
  In vitroperturbation biology, proteomics and Bayesian causal analysis identified biomarkers that were validated inin vitromodels and clinical specimens.
  
  
  RESULTS
  Filamin-B (FLNB) and Keratin-19 were identified as biomarkers. Filamin-A (FLNA) was found to be causally linked to FLNB. Characterization of the biomarkers in a panel of cells revealed differential mRNA expression and regulation. Moreover, FLNA and FLNB were detected in the conditioned media of cells. Last, in patients without PrCa, FLNA and FLNB blood levels were positively correlated, while in patients with adenocarcinoma the relationship is dysregulated.
  
  
  CONCLUSIONS
  These data support the strategy and the potential use of the biomarkers for PrCa.
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Niven R
authors.initials: NR
authors.lastname: Narain
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Anne R
authors.initials: AR
authors.lastname: Diers
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Arleide
authors.initials: A
authors.lastname: Lee
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Socheata
authors.initials: S
authors.lastname: Lao
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Joyce Y
authors.initials: JY
authors.lastname: Chan
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Sally
authors.initials: S
authors.lastname: Schofield
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Joe
authors.initials: J
authors.lastname: Andreazi
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Rakibou
authors.initials: R
authors.lastname: Ouro-Djobo
authors.affiliation: Department of Dermatology, University of Miami Miller School of Medicine, Miami, FL, USA 33136; Department of Dermatology, University of Miami Miller School of Medicine, Miami, FL, USA 33136.
authors.forename: Joaquin J
authors.initials: JJ
authors.lastname: Jimenez
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Tracey
authors.initials: T
authors.lastname: Friss
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Nikunj
authors.initials: N
authors.lastname: Tanna
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Aditee
authors.initials: A
authors.lastname: Dalvi
authors.affiliation: Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, USA 44195; Department of Chemistry, Cleveland State University, Cleveland, OH, USA 44115; Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, USA 44195; Department of Chemistry, Cleveland State University, Cleveland, OH, USA 44115.
authors.forename: Sihe
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, USA 44195; Department of Chemistry, Cleveland State University, Cleveland, OH, USA 44115; Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, USA 44195; Department of Chemistry, Cleveland State University, Cleveland, OH, USA 44115.
authors.forename: Dustin
authors.initials: D
authors.lastname: Bunch
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Yezhou
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Khampaseuth
authors.initials: K
authors.lastname: Thapa
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Stephane
authors.initials: S
authors.lastname: Gesta
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Leonardo O
authors.initials: LO
authors.lastname: Rodrigues
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Viatcheslav R
authors.initials: VR
authors.lastname: Akmaev
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Vivek K
authors.initials: VK
authors.lastname: Vishnudas
authors.affiliation: Berg, LLC, Framingham, MA, USA; Berg, LLC, Framingham, MA, USA.
authors.forename: Rangaprasad
authors.initials: R
authors.lastname: Sarangarajan
keywords: Bayesian network learning; FLNA; FLNB; KRT19; biomarkers; prostate cancer