title: BNIP3, a cell pro-apoptotic protein, involved in response to viral infection in orange spotted grouper, Epinephelus coioides.
abstract: BNIP3 is a kind of BH3-only protein that induces both cell death and autophagy. Here, a BNIP3 gene (EcBNIP3) was identified from orange spotted grouper, Epinephelus coioides. EcBNIP3 possessed 236 amino acids residues, contained a conservative BNIP3 domain and a transmembrane region. Besides, EcBNIP3 expressed at a relative high level in heart and spleen. EcBNIP3 transcript was up-regulated after SGIV infection in vitro. Subcellular localization analysis revealed that EcBNIP3 was predominantly localized in the cytoplasm and co-localized with mitochondria. In addition, overexpression EcBNIP3 accelerated SGIV infection induced cell death but inhibited viral genes transcription. Taken together, these results provided new evidence that fish BNIP3 might involved in response to virus infection.
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, PR China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cai
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
authors.forename: Shina
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, PR China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, PR China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Yu
authors.affiliation: Hunan Fisheries Science Institute, Changsha 410153, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Song
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, PR China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, PR China.
authors.forename: Yishan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, PR China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, PR China.
authors.forename: Zaohe
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China; College of Marine Sciences, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: qinqiwei64@outlook.com.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Qin
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, PR China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, PR China. Electronic address: jianjc@gdou.edu.cn.
authors.forename: Jichang
authors.initials: J
authors.lastname: Jian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001492:Bass; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017995:Ranavirus / Q000502:physiology*; D016415:Sequence Alignment / Q000662:veterinary; D014018:Tissue Distribution
chemical_list: D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger
keywords: BNIP3; Epinephelus coioides; Singapore grouper iridovirus (SGIV); Viral infection