title: One mechanism of glucocorticoid action in asthma may involve the inhibition of IL-25 expression.
abstract: While the mechanism of action of classic cytokines in asthma has received increased attention from researchers, certain non-classical cytokines, such as IL-25, also participate in this mechanism. The present study was performed to investigate the changes in IL-25 (IL-17E) mRNA and protein in bronchial asthma and to further characterize the mechanism underlying the action of glucocorticoids in asthma. A total of 96 specific pathogen-free BALB/c male mice were randomly divided into three normal groups (after the first allergization, after the second allergization and after excitation), three asthma groups (with the same three subgroups), a dexamethasone group and a budesonide group (n=12/group). An asthma model was established via the ovalbumin-sensitized excitation method. Mice in the dexamethasone group received intraperitoneal injections of dexamethasone 1 h prior to each excitation, the budesonide group received a budesonide suspension via inhalation 2 h before and after each provocation, and the normal group was sensitized and challenged with isotonic saline. IL-25 protein expression levels in the bronchoalveolar lavage fluid were measured by ELISA, and the relative IL-25 mRNA content in lung tissue was determined by reverse transcription-quantitative polymerase chain reaction. Compared with the normal groups, both the protein and mRNA levels of IL-25 were significantly increased (P<0.05) in the asthma groups. Dexamethasone and budesonide groups exhibited significant protein and mRNA reductions in IL-25, as compared with the asthma group after excitation (P<0.05), whereas these two groups significantly increased levels compared with the normal group after excitation (P<0.05). No significant differences in IL-25 mRNA expression levels were detected in the dexamethasone and budesonide groups when compared with the normal group after excitation. Therefore, we conclude that IL-25 is involved throughout the process of inflammation and inflammatory immune pathogenesis in asthma. One of the mechanisms of glucocorticoid action in asthma may involve inhibition of IL-25 expression.
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Chengming
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Zhang
keywords: asthma; budesonide; dexamethasone; glucocorticoid; interleukin-25