title: Pinus massonianabark extract inhibits migration of the lung cancer A549 cell line.
abstract: The bark of Pinus massoniana is a traditional Chinese medicine for the treatment of various health disorders. Previous studies have demonstrated that P. massoniana bark extract (PMBE) may induce the apoptosis of hepatoma and cervical cancer cells. However, whether PMBE is able to inhibit the migration of lung cancer cells requires further investigation. In the current study, the effects of PMBE on the viability of human lung cancer A549 cells were detected using an MTT assay. The migration of lung cancer cells following exposure to PMBE were quantified using wound healing and Transwell assays, respectively. The expression levels of matrix metalloproteinase (MMP)-9 were determined using western blotting. The results revealed that PMBE significantly inhibited the growth of the lung cancer cells. In addition, the wound closure rate and the migration of the lung cancer cells were suppressed by PMBE. Furthermore, the expression levels of MMP-9 were reduced. These findings indicated that PMBE is able to restrict the migration and invasion of lung cancer cells, and that PMBE may serve as a novel therapeutic agent for patients with metastatic lung cancer in the future.
authors.affiliation: Department of General Surgery, The People's Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Mao
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Ershao
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Likun
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The People's Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Daqing
authors.initials: D
authors.lastname: Rong
authors.affiliation: Department of General Surgery, The People's Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
keywords: Pinus massoniana bark extract; lung cancer; matrix metalloproteinase-9; migration