title: Alpha-bisabolol Promotes Glioma Cell Death by Modulating the Adenosinergic System.
abstract: Glioblastoma multiforme is the most malignant type of glioma. Alpha-bisabolol is an essential oil reported as a potent cell death agent. In the present work, we evaluated the effect of alpha-bisabolol on ecto-5'-nucleotidase/CD73, the most well-characterized enzymatic source of adenosine, present in lipid rafts.
  
  
  
  Glioma cells were treated with alpha-bisabolol and, in some experiments, pre-treated with an A3antagonist. MTT assay (viability), malachite green method (ecto-5'-nucleotidase/CD73 activity) and quantitative polymerase chain reaction (qPCR) (A3mRNA) were carried out.
  
  
  
  Alpha-bisabolol led to a decrease in C6 and U138-MG glioma cells viability, accompanied by an increase in ecto-5'-NT/CD73 activity. Pre-treatment with an A3antagonist reverted the effect of α-bisabolol with an increase of mRNA expression of this receptor.
  
  
  
  Our data indicated the participation of ecto-5'-nucleotidase/CD73 and A3receptor in the anti-proliferative effect of α-bisabolol on glioma cells.
authors.affiliation: Graduate Program in Biological Sciences - Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Franciane Brackmann
authors.initials: FB
authors.lastname: Mendes
authors.affiliation: Graduate Program in Biological Sciences - Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Letícia Scussel
authors.initials: LS
authors.lastname: Bergamin
authors.affiliation: Graduate Program in Biological Sciences - Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Dos Santos Stuepp
authors.affiliation: Graduate Program in Biological Sciences - Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Elizandra
authors.initials: E
authors.lastname: Braganhol
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Thatiana
authors.initials: T
authors.lastname: Terroso
authors.affiliation: Department of Organic Chemistry, Institute of Chemistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Adriana Rafin
authors.initials: AR
authors.lastname: Pohlmann
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Sílvia Stanisçuaski
authors.initials: SS
authors.lastname: Guterres
authors.affiliation: Graduate Program in Biological Sciences - Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil abattastini@gmail.com.|Department of Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Ana Maria Oliveira
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D000081222:Monocyclic Sesquiterpenes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D043684:Receptor, Adenosine A3 / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000081222:Monocyclic Sesquiterpenes; D012333:RNA, Messenger; D043684:Receptor, Adenosine A3; D012717:Sesquiterpenes; C004497:bisabolol; D015720:5'-Nucleotidase
keywords: Glioma; adenosine; ecto-5’-NT/CD73; α-bisabolol