title: Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant byBacillus subtilisPF1 using agro industrial waste.
abstract: The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design expert software which resulted in a 1.2, 0.84 and 2.28 fold increase in protease, amylase and biosurfactant production. The proteolytic activity was found to be optimum at pH 9.0 and 60 °C while optimum amylolytic activity was recorded at pH 6.0 and 70 °C respectively. Both enzymes were found to be stable in the presence of organic solvents, ionic and commercial detergent and oxidizing agents. The biosurfactant was extracted with chloroform and was found to be stable at varying pH and temperature; however a reduction in the activity was observed at temperature higher than 70 °C. The isolated enzymes and biosurfactants may find applications in the effective removal of stains.
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 495009, Chhattisgarh, India.
authors.forename: Khushboo
authors.initials: K
authors.lastname: Bhange
authors.affiliation: School of Biotechnology, Banaras Hindu University, Varanasi, U.P., India.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Chaturvedi
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur 495009, Chhattisgarh, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Bhatt
keywords: Amylase; Bacillus subtilis PF1; Biosurfactant; Protease