title: miR-199a-3p downregulation in thyroid tissues is associated with invasion and metastasis of papillary thyroid carcinoma.
abstract: OBJECTIVE
  miR-199a-3p may play an important role in tumour inhibition. The aim of the present study was to determine any link between miR-199a-3p expression in thyroid tissues and clinicopathologic features, as well as to assess potential usefulness of miR-199a-3p in prediction for invasion and metastasis of papillary thyroid carcinoma (PTC).
  
  
  METHODS
  A total of 188 tissue samples (136 PTCs, 52 normal thyroid tissue) were collected. We measured the levels of miR-199a-3p with quantitative reverse-transcriptase PCR (RT-qPCR) in all subjects. In addition, the correlation between the expression levels of miR-199a-3p and clinicopathological factors was explored.
  
  
  RESULTS
  qRT-PCR indicated that the expression levels of miR-199a-3p was 7.1 (95% CI, 3.9-12.4) in PTCs, which was significantly lower than that of in the normal thyroid tissues 31.4 (95% CI, 15.4-44.3) (p = 0.002). Receiver operating characteristic curve (ROC) analyses revealed that miR-199a-3p could be promising biomarkers for PTC, with relatively high area under the curve (AUC) values was 0.87 (95% CI, 0.66-0.90; p = 0.001). Low miR-199a-3p expression levels were linked to TNM stage (p = 0.026), extra-thyroidal extension (p = 0.02), lymph node (LN) metastasis (p = 0.036), distant metastasis (p = 0.002) and recurrence of LN metastasis (p = 0.03).
  
  
  CONCLUSIONS
  Our data suggest that downregulation of miR-199a-3p in thyroid tissues is linked to invasion and metastasis of PTC and may be a potential target for therapeutic intervention.
authors.affiliation: a Department of General Surgery , The Central Hospital of Yishui , Linyi , China.
authors.forename: Chengbiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: b Department of Blood Transfusion , Yantai Yu-huang-ding Hospital , Yantai , China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Xing
authors.affiliation: c Department of Blood transfusion , People's Hospital of Weifang , Weifang , China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: d Department of Thyroid Surgery , The Affiliated Hospital of Qingdao University , Qingdao , China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Zhang
mesh_terms: D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012372:ROC Curve; D000077273:Thyroid Cancer, Papillary / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D013961:Thyroid Gland / Q000473:pathology*
chemical_list: D035683:MicroRNAs; C531408:mirn199 microRNA, human
keywords: Papillary thyroid carcinoma; metastasis, miR-199a-3p