title: Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection.
abstract: A monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ornidazole (ONZ) detection were developed. ONZ was conjugated with cationic bovine serum albumin as a hapten to generate the artificial immunogens and coating antigens. BALB/c mice were immunized, and mAbs were obtained. The competitive inhibition curve of ic-ELISA was y=0.0438x2-0.2101x+0.2925, with R2=0.9941. The 50% inhibition concentration, the limit of detection, and limit of quality for ONZ were 0.15, 0.01, and 0.05µg/kg, respectively. The cross-reactivity of the mAbs to secnidazole was 0.33%. The recoveries were from 89.18% to 101.63% and the coefficient of variation was less than 7.15% in chicken, chicken liver, and honey samples, all of which had ONZ concentrations of 0.05 and 0.1μg/kg. Results showed that the ic-ELISA based on mAb could be used for the rapid detection for ONZ.
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Tiangang
authors.initials: T
authors.lastname: Wang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Jin
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100083, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Ebeydulla
authors.initials: E
authors.lastname: Rahman
authors.affiliation: Beijing Weifengyongzhu Technology Developing Co., Ltd., District A, Beijing 100020, People's Republic of China; Pingyuan County Weifengyongzhu Technology Co., Ltd., Shandong Province, Dezhou City 252100, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Ji
authors.affiliation: Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China. Electronic address: zf@cau.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D009950:Ornidazole / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D009950:Ornidazole
keywords: ELISA; Monoclonal antibodies; Ornidazole