title: Production of surfactin from waste distillers' grains by co-culture fermentation of two Bacillus amyloliquefaciens strains.
abstract: Distillers' grains (DGS), the main waste by-products of Chinese liquor industry, were used as substrate for surfactin production. Bacillus amyloliquefaciens MT45 could grow with DGS as sole carbon source to produce 1.04g/l surfactin. However, low amylase activity of MT45 limited sugar supply and the subsequent surfactin production. Therefore, MT45 was co-cultured with Bacillus strains that exhibited remarkable hydrolases activities. Surfactin yield increased by 50% when MT45 was co-cultured with B. amyloliquefaciens X82 that showed no product inhibition effect and did not develop extracellular matrix. The inoculation ratio of X82 greatly influenced the sugar supply, cellular growth, and surfactin production of the co-culture fermentation. Maximum surfactin titration (3.4g/l) was obtained when MT45 and X82 were co-cultured with inoculation ratio at 1:0.5, using 200g/l DGS. This work highlights the feasibility of using industrial waste DGS as promising feedstocks to produce value-added surfactin by co-culture fermentation.
authors.affiliation: State Key Laboratory of Food Science and Technology, The Key Laboratory of Industrial Biotechnology, Synergetic Innovation Centre of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: State Key Laboratory of Food Science and Technology, The Key Laboratory of Industrial Biotechnology, Synergetic Innovation Centre of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology, The Key Laboratory of Industrial Biotechnology, Synergetic Innovation Centre of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: yxu@jiangnan.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D001407:Bacillus; D000069977:Bacillus amyloliquefaciens; D002241:Carbohydrates; D018920:Coculture Techniques; D005285:Fermentation; D007220:Industrial Waste
chemical_list: D002241:Carbohydrates; D007220:Industrial Waste
keywords: Bacillus amyloliquefaciens; Co-culture fermentation; Distillers’ grains; Surfactin