title: Response of the rhizosphere prokaryotic community of barley (Hordeum vulgare L.) to elevated atmospheric CO2concentration in open-top chambers.
abstract: The effect of elevated atmospheric CO2concentration [CO2] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) from six chambers with ambient [CO2] (approximately 400 ppm) and six chambers with elevated [CO2] (700 ppm). The V4 region of the 16S rRNA gene was PCR-amplified from the extracted DNA and sequenced on an Illumina MiSeq instrument. Above-ground plant biomass was not affected by elevated [CO2] at anthesis, but plants exposed to elevated [CO2] had significantly higher grain yield. The composition of the rhizosphere prokaryotic communities was very similar under ambient and elevated [CO2]. The dominant taxa were Bacteroidetes, Actinobacteria, Alpha-, Gamma-, and Betaproteobacteria. Elevated [CO2] resulted in lower prokaryotic diversity in the rhizosphere, but did not cause a significant difference in community structure.
authors.affiliation: Thünen Institute of Biodiversity, Braunschweig, Germany.
authors.forename: Márton
authors.identifier: 0000-0002-7264-3369
authors.initials: M
authors.lastname: Szoboszlay
authors.affiliation: Thünen Institute of Biodiversity, Braunschweig, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Näther
authors.affiliation: Thünen Institute of Biodiversity, Braunschweig, Germany.
authors.forename: Esther
authors.initials: E
authors.lastname: Mitterbauer
authors.affiliation: Thünen Institute of Biodiversity, Braunschweig, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bender
authors.affiliation: Thünen Institute of Biodiversity, Braunschweig, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Weigel
authors.affiliation: Thünen Institute of Biodiversity, Braunschweig, Germany.
authors.forename: Christoph C
authors.identifier: 0000-0003-4861-0214
authors.initials: CC
authors.lastname: Tebbe
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058448:Biota / Q000187:drug effects*; D002245:Carbon Dioxide / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D001467:Hordeum / Q000254:growth & development; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002245:Carbon Dioxide
keywords: 16S rRNA amplicon sequencing; Barley; atmospheric CO2; climate change; prokaryotic diversity; rhizosphere