title: Structural characterization and evaluation of the antioxidant activities of polysaccharides extracted from Qingzhuan brick tea.
abstract: The crude tea polysaccharides (CTPS) from Qingzhuan brick tea(QZBT) were extracted and fractionated to afford two fractions, namely TPS-1 and TPS-2. Analyses were conducted concerning the structural characterization and antioxidant activities of these samples. Component analysis revealed that the carbohydrate, uronic acid, protein and polyphenol contents of these samples differed significantly. Fourier transform infrared analysis showed that these samples showed similar characteristic absorption peaks for polysaccharides. Ultraviolet-visible spectroscopy, circular dichroism, scanning electron microscopy and thermogravimetric analyses indicated that there were considerable differences in the presence of protein, surface features, conformational characteristics and thermodynamic behaviors. For antioxidant activities in vitro, CTPS, TPS-1 and TPS-2 exhibited concentration-dependent antioxidant activities, with TPS-2 showing significantly higher antioxidant activity than CTPS and TPS-1. These results provide a scientific and strong foundation for the use of tea polysaccharides(TPS) from QZBT and further research towards the relationships between the characteristics and antioxidant activities of TPS.
authors.affiliation: Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan, Hubei 432000, PR China. Electronic address: hbxhyang@163.com.
authors.forename: Xinhe
authors.initials: X
authors.lastname: Yang
authors.affiliation: Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan, Hubei 432000, PR China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Huang
authors.affiliation: Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan, Hubei 432000, PR China.
authors.forename: Caiqin
authors.initials: C
authors.lastname: Qin
authors.affiliation: Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan, Hubei 432000, PR China.
authors.forename: Bangyu
authors.initials: B
authors.lastname: Lv
authors.affiliation: Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan, Hubei 432000, PR China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Hubei Provincial Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hubei Engineering University, Xiaogan, Hubei 432000, PR China; Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, PR China. Electronic address: larkin-liu@163.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D005285:Fermentation; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry; D013662:Tea / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; D013662:Tea; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron
keywords: Antioxidant activity; Polysaccharides of Qingzhuan brick tea; Structure