title: Insights into metabolism and sodium chloride adaptability of carbaryl degrading halotolerant Pseudomonas sp. strain C7.
abstract: Pseudomonas sp. strain C7 isolated from sediment of Thane creek near Mumbai, India, showed the ability to grow on glucose and carbaryl in the presence of 7.5 and 3.5% of NaCl, respectively. It also showed good growth in the absence of NaCl indicating the strain to be halotolerant. Increasing salt concentration impacted the growth on carbaryl; however, the specific activity of various enzymes involved in the metabolism remained unaffected. Among various enzymes, 1-naphthol 2-hydroxylase was found to be sensitive to chloride as compared to carbaryl hydrolase and gentisate 1,2-dioxygenase. The intracellular concentration of Cl-ions remained constant (6-8 mM) for cells grown on carbaryl either in the presence or absence of NaCl. Thus the ability to adapt to the increasing concentration of NaCl is probably by employing chloride efflux pump and/or increase in the concentration of osmolytes as mechanism for halotolerance. The halotolerant nature of the strain will be beneficial to remediate carbaryl from saline agriculture fields, ecosystems and wastewaters.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Vikas D
authors.initials: VD
authors.lastname: Trivedi
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Anahita
authors.initials: A
authors.lastname: Bharadwaj
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Madhushri S
authors.initials: MS
authors.lastname: Varunjikar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Arminder K
authors.initials: AK
authors.lastname: Singha
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Upadhyay
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Kamini
authors.initials: K
authors.lastname: Gautam
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India. pphale@iitb.ac.in.
authors.forename: Prashant S
authors.initials: PS
authors.lastname: Phale
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D012721:Carbaryl / Q000378:metabolism*; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005841:Gentisates / Q000378:metabolism; D007194:India; D007306:Insecticides / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005841:Gentisates; D007306:Insecticides; D012965:Sodium Chloride; D049308:Dioxygenases; C026247:gentisate 1,2-dioxygenase; D012721:Carbaryl
keywords: 1-Naphthol 2-hydroxylase; Adaptability; Carbaryl metabolism; Halotolerance; Intracellular Cl− ions; Pseudomonas