title: Methionine-mediated gene expression and characterization of the CmhR regulon inStreptococcus pneumoniae.
abstract: This study investigated the transcriptomic response ofStreptococcus pneumoniaeD39 to methionine. Transcriptome comparison of theS. pneumoniaeD39 wild-type grown in chemically defined medium with 0-10 mM methionine revealed the elevated expression of various genes/operons involved in methionine synthesis and transport (fhs,folD,gshT,metA,metB-csd,metEF,metQ,tcyB,spd-0150,spd-0431andspd-0618). Furthermore,β-galactosidase assays and quantitative RT-PCR studies demonstrated that the transcriptional regulator, CmhR (SPD-0588), acts as a transcriptional activator of thefhs,folD,metB-csd,metEF,metQandspd-0431genes. A putative regulatory site of CmhR was identified in the promoter region of CmhR-regulated genes and this CmhR site was further confirmed by promoter mutational experiments.
authors.affiliation: 1​Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands.|2​Department of Bioinformatics and Biotechnology, G C University, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
authors.affiliation: 3​Department of Microbiology, Tumor and Cell Biology, , Karolinska Institutet, Nobels väg 16, Stockholm, SE-171 77, Sweden.
authors.forename: Sulman
authors.initials: S
authors.lastname: Shafeeq
authors.affiliation: 1​Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands.
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008715:Methionine / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D018085:Regulon / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D008715:Methionine
keywords: CmhR; MetE; MetQ; Methionine; Pneumococcus