title: Zizyphin modulates calcium signalling in human taste bud cells and fat taste perception in the mouse.
abstract: Zizyphin, isolated from Zizyphus sps. leaf extracts, has been shown to modulate sugar taste perception, and the palatability of a sweet solution is increased by the addition of fatty acids. We, therefore, studied whether zizyphin also modulates fat taste perception. Zizyphin was purified from edible fruit of Zizyphus lotus L. Zizyphin-induced increases in [Ca2+]i in human taste bud cells (hTBC). Zizyphin shared the endoplasmic reticulum Ca2+pool and also recruited, in part, Ca2+from extracellular environment via the opening of store-operated Ca2+channels. Zizyphin exerted additive actions on linoleic acid (LA)-induced increases in [Ca2+]i in these cells, indicating that zizyphin does not exert its action via fatty acid receptors. However, zizyphin seemed to exert, at least in part, its action via bile acid receptor Takeda-G-protein-receptor-5 in hTBC. In behavioural tests, mice exhibited preference for both LA and zizyphin. Interestingly, zizyphin increased the preference for a solution containing-LA. This study is the first evidence of the modulation of fat taste perception by zizyphin at the cellular level in hTBC. Our study might be helpful for considering the synthesis of zizyphin analogues as 'taste modifiers' with a potential in the management of obesity and lipid-mediated disorders.
authors.affiliation: Physiologie de la Nutrition & Toxicologie, UMR U866 INSERM/Université de Bourgogne-Franche Compté (BFC)/Agro-Sup, Dijon, 21000, France.
authors.forename: Babar
authors.initials: B
authors.lastname: Murtaza
authors.affiliation: LAPRONA, Université Aboubekr Belkaid, Tlemcen, 13000, Algeria.
authors.forename: Meryem
authors.initials: M
authors.lastname: Berrichi
authors.affiliation: LAPRONA, Université Aboubekr Belkaid, Tlemcen, 13000, Algeria.
authors.forename: Chahid
authors.initials: C
authors.lastname: Bennamar
authors.affiliation: Interactions Cellulaires et Physiopathologie Hépatique, UMR 1174INSERM/Université Paris Sud, 91405, Orsay Cedex, Paris, USA.
authors.forename: Thierry
authors.initials: T
authors.lastname: Tordjmann
authors.affiliation: LAPRONA, Université Aboubekr Belkaid, Tlemcen, 13000, Algeria.
authors.forename: Fatima Z
authors.initials: FZ
authors.lastname: Djeziri
authors.affiliation: Physiologie de la Nutrition & Toxicologie, UMR U866 INSERM/Université de Bourgogne-Franche Compté (BFC)/Agro-Sup, Dijon, 21000, France.
authors.forename: Aziz
authors.initials: A
authors.lastname: Hichami
authors.affiliation: Physiologie de la Nutrition & Toxicologie, UMR U866 INSERM/Université de Bourgogne-Franche Compté (BFC)/Agro-Sup, Dijon, 21000, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Leemput
authors.affiliation: LAPRONA, Université Aboubekr Belkaid, Tlemcen, 13000, Algeria.
authors.forename: Meriem
authors.initials: M
authors.lastname: Belarbi
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104-3308, USA.
authors.forename: Hakan
authors.initials: H
authors.lastname: Ozdener
authors.affiliation: Physiologie de la Nutrition & Toxicologie, UMR U866 INSERM/Université de Bourgogne-Franche Compté (BFC)/Agro-Sup, Dijon, 21000, France.
authors.forename: Naim A
authors.identifier: http://orcid.org/0000-0002-8930-9332
authors.initials: NA
authors.lastname: Khan
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004041:Dietary Fats / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D013650:Taste Buds / Q000187:drug effects* / Q000378:metabolism; D055697:Taste Perception / Q000187:drug effects* / Q000502:physiology; D031957:Ziziphus
chemical_list: D000470:Alkaloids; D004041:Dietary Fats; C473772:GPBAR1 protein, human; D010456:Peptides, Cyclic; D010936:Plant Extracts; D043562:Receptors, G-Protein-Coupled; C432974:zizyphine A
keywords: Zizyphus lotus L; fat taste; sweet taste; zizyphin