title: A Smart Molecule for Selective Sensing of Nitric Oxide: Conversion of NO to HSNO; Relevance of Biological HSNO Formation.
abstract: A smart molecule, QT490, containing thiosemicarbazide moiety acts as a highly selective turn-on in vitro NO sensor through the unprecedented NO-induced transformation of thiosemicarbazide moiety to 1,3,4-oxadiazole heterocycle with the concomitant release of HSNO, thereby eliminating any interference from various endogenous biomolecules including dehydroascorbic acid, ascorbic acid, etc. The kinetic studies of the reactions between QT490and NO provide a mechanistic insight into formation of HSNO/RSNO from the reaction between H2S/RSH and NO in the biological system. This novel probe is non-cytotoxic, cell permeable, water-soluble, and appropriate for intracellular cytoplasmic NO sensing with the possibilities of in vivo applications.
authors.affiliation: Department of Chemistry, Jadavpur University , 188 Raja S.C. Mallick Road, Kolkata 700 032, India.
authors.forename: Abu Saleh Musha
authors.initials: ASM
authors.lastname: Islam
authors.affiliation: Department of Chemistry, Jadavpur University , 188 Raja S.C. Mallick Road, Kolkata 700 032, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Bhowmick
authors.affiliation: Department of Chemistry, Jadavpur University , 188 Raja S.C. Mallick Road, Kolkata 700 032, India.
authors.forename: Kaberi
authors.initials: K
authors.lastname: Pal
authors.affiliation: Molecular & Human Genetics Division, CSIR-Indian Institute of Chemical Biology , 4 Raja S.C. Mallick Road, Kolkata 700 032, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Katarkar
authors.affiliation: Molecular & Human Genetics Division, CSIR-Indian Institute of Chemical Biology , 4 Raja S.C. Mallick Road, Kolkata 700 032, India.
authors.forename: Keya
authors.initials: K
authors.lastname: Chaudhuri
authors.affiliation: Department of Chemistry, Jadavpur University , 188 Raja S.C. Mallick Road, Kolkata 700 032, India.
authors.forename: Mahammad
authors.identifier: http://orcid.org/0000-0003-0756-0468
authors.initials: M
authors.lastname: Ali
mesh_terms: D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D009569:Nitric Oxide / Q000737:chemistry*; D026403:S-Nitrosothiols / Q000138:chemical synthesis* / Q000737:chemistry; D012663:Semicarbazides / Q000737:chemistry*
chemical_list: D026403:S-Nitrosothiols; D012663:Semicarbazides; D009569:Nitric Oxide; C005151:thiosemicarbazide