title: A Protocol for Producing the Maternal Low-Protein Rat Model: A Tool for Preclinical Proteomic Studies.
abstract: Epidemiological studies have shown that periods of poor nutrition during pregnancy can lead to an increased risk of metabolic and psychiatric disorders in the offspring. In addition, some individuals with disorders such as schizophrenia show signs of impaired insulin signalling. These findings indicate that there may be a link between metabolism and neuronal function and they also provide supporting evidence for neurodevelopmental origins of mental illness. This chapter gives a detailed protocol for generation of the maternal low-protein (LP) rat model, which leads to metabolic disturbances in the offspring. This model has been used in proteomic studies of several disorders including schizophrenia.
authors.affiliation: Department of neurosciences, University of Cambridge, Clifford Allbutt building, Hills road, Cambridge, CB2 0AH, UK.
authors.forename: Dan
authors.initials: D
authors.lastname: Ma
authors.affiliation: University of Cambridge Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, Addenbrooke's Hospital, Cambridge, UK, CB2 0QQ.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Ozanne
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), RuaMonteiroLobato 255 F/01, CidadeUniversitáriaZeferinoVaz, 13083-862, Campinas, Brazil. paulcguest@yahoo.co.uk.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D007774:Lactation; D011247:Pregnancy; D011248:Pregnancy Complications / Q000209:etiology / Q000503:physiopathology*; D011297:Prenatal Exposure Delayed Effects; D011502:Protein-Energy Malnutrition / Q000209:etiology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D012559:Schizophrenia / Q000209:etiology*
keywords: Biomarkers; Low-protein model; Metabolic programming; Preclinical proteomics; Schizophrenia