title: Valorization of spent oyster mushroom substrate and laccase recovery through successive solid state cultivation of Pleurotus, Ganoderma, and Lentinula strains.
abstract: Spent mushroom substrate (SMS) of Pleurotus ostreatus was supplemented with wheat bran and soybean flour in various proportions to obtain C/N ratios of 10, 20, and 30, and their effect was evaluated in successive cultivation of Pleurotus ostreatus, Pleurotus pulmonarius, Ganoderma adspersum, Ganoderma resinaceum, and Lentinula edodes strains with respect to mycelium growth rate, biomass concentration, recovery of the enzyme laccase and crude exopolysaccharides, and also with additional fruiting body production. All fungi showed the highest growth rate on unamended SMS (C/N 30), with G. resinaceum being the fastest colonizer (Kr = 9.84 mm day-1), while biomass concentration maximized at C/N 10. Moreover, supplementation affected positively laccase activity, with P. pulmonarius furnishing the highest value (44,363.22 U g-1) at C/N 20. On the contrary, L. edodes growth, fruiting, and laccase secretion were not favored by SMS supplementation. Fruiting body formation was promoted at C/N 30 for Ganoderma and at C/N 20 for Pleurotus species. Exopolysaccharide production of further studied Pleurotus strains was favored at a C/N 20 ratio, at the initial stage of SMS colonization. The obtained results support the potential effective utilization of supplemented SMS for laccase production from Ganoderma spp. and for new fruiting body production of Pleurotus spp.
authors.affiliation: Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization-Demeter, 1 Sofokli Venizelou Street, 14123, Lykovryssi, Attiki, Greece.
authors.forename: Christina N
authors.initials: CN
authors.lastname: Economou
authors.affiliation: Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization-Demeter, 1 Sofokli Venizelou Street, 14123, Lykovryssi, Attiki, Greece. sdiamantopoulou@gmail.com.
authors.forename: Panagiota A
authors.initials: PA
authors.lastname: Diamantopoulou
authors.affiliation: Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization-Demeter, 1 Sofokli Venizelou Street, 14123, Lykovryssi, Attiki, Greece.
authors.forename: Antonios N
authors.initials: AN
authors.lastname: Philippoussis
mesh_terms: D000363:Agaricales / Q000737:chemistry; D018533:Biomass; D045424:Complex Mixtures / Q000494:pharmacology*; D004043:Dietary Fiber / Q000494:pharmacology; D005285:Fermentation; D005433:Flour; D048690:Fruiting Bodies, Fungal / Q000187:drug effects / Q000254:growth & development; D032902:Ganoderma / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D042845:Laccase / Q000096:biosynthesis* / Q000378:metabolism; D020074:Lentinula / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D020076:Pleurotus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013025:Glycine max / Q000737:chemistry
chemical_list: D045424:Complex Mixtures; D004043:Dietary Fiber; D042845:Laccase
keywords: Biomass; Exopolysaccharides; Fruiting body; Glass tubes; Growth rate; Solid state fermentation