title: Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations.
abstract: The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population. Other two populations of Bryconamericus sp. from the Vermelho stream/PR and Cambuta river/PR exhibited interpopulation variation. The chromosome mapping of rDNA sites revealed unique markings among the three populations, showing inter- and intrapopulation variability located in the terminal region. The molecular analysis using DNA barcoding complementing the cytogenetic analysis also showed differentiation among the three populations. The U2 small nuclear DNA repetitive sequence exhibited conserved features, being located in the interstitial region of a single chromosome pair. This is the first report on its occurrence in the genus Bryconamericus. Data obtained revealed a karyotype variability already assigned to the genus, along with polymorphism of ribosomal sites, demonstrating that this group of fish can be undergoing a divergent evolutionary process, constituting a substantive model for studies of chromosomal evolution.
authors.affiliation: 1 Laboratório de Citogenética Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .
authors.forename: Angélica Rossotti Dos
authors.initials: ARD
authors.lastname: Santos
authors.affiliation: 1 Laboratório de Citogenética Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .
authors.forename: Mariana Campaner
authors.initials: MC
authors.lastname: Usso
authors.affiliation: 1 Laboratório de Citogenética Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .
authors.forename: Juceli Gonzalez
authors.initials: JG
authors.lastname: Gouveia
authors.affiliation: 2 Laboratório de Citogenética de Vertebrados, ICBM Facultad de Medicina, Universidad de Chile , Santiago, Chile .|3 Laboratório de Genética e Ecologia Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .|4 Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista , Botucatu, Brazil .
authors.forename: Cristian
authors.initials: C
authors.lastname: Araya-Jaime
authors.affiliation: 3 Laboratório de Genética e Ecologia Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .
authors.forename: Wilson
authors.initials: W
authors.lastname: Frantine-Silva
authors.affiliation: 1 Laboratório de Citogenética Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .
authors.forename: Lucia
authors.initials: L
authors.lastname: Giuliano-Caetano
authors.affiliation: 4 Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista , Botucatu, Brazil .
authors.forename: Fausto
authors.initials: F
authors.lastname: Foresti
authors.affiliation: 1 Laboratório de Citogenética Animal, Departamento de Biologia Geral, Universidade Estadual de Londrina , Londrina, Brazil .
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Dias
mesh_terms: D000818:Animals; D060126:Characidae / Q000145:classification* / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D014644:Genetic Variation; D005828:Genetics, Population; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D012091:Repetitive Sequences, Nucleic Acid
keywords: U2 snDNA; fish cytogenetics; karyotypic polymorphism; molecular cytogenetics; ribosomal DNA