title: The lipopolysaccharide lipid A structure from the marine sponge-associated bacterium Pseudoalteromonas sp. 2A.
abstract: Suberites domuncula is a marine demosponge harbouring a large bacterioflora, including commensal, opportunistic and pathogenic bacteria, among which, species of the Gram-negative genus Pseudoalteromonas were identified. The sponge-bacteria interaction mechanisms are still not fully understood. As the main component of the Gram-negative bacterial outer membrane, the lipopolysaccharide (LPS) may play a role in such a crucial relationship. Moreover, the LPS is known to be the most versatile bioactive macromolecule of Gram-negative bacteria and its lipid A structure is responsible for the immunological activity of the whole LPS on eukaryotic host cells. Here it is reported the structural characterisation of the LPS lipid A moiety isolated from the S. domuncula-associated commensal bacterium, Pseudoalteromonas sp. 2A. Chemical and MALDI mass spectrometry analyses, performed on both the LPS and the isolated lipid A as well as on the intact bacterial cells, highlighted a complex family of penta-acylated lipid A species carrying two phosphate units on the disaccharide backbone.
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cinthia 4, 80126, Naples, Italy. flaviana.dilorenzo@unina.it.
authors.forename: Flaviana
authors.initials: F
authors.lastname: Di Lorenzo
mesh_terms: D000818:Animals; D005227:Fatty Acids / Q000737:chemistry; D008050:Lipid A / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010766:Phosphorylation; D031161:Pseudoalteromonas / Q000737:chemistry* / Q000166:cytology; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D047233:Suberites / Q000382:microbiology*; D013559:Symbiosis
chemical_list: D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides
keywords: Innate immunity; Lipid A; Lipopolysaccharide; Pseudoalteromonas sp.; Structural characterisation; Suberites domuncula