title: Relationship of Microbiota and Cyanobacterial Secondary Metabolites in Planktothricoides-Dominated Bloom.
abstract: The identification of phytoplankton species and microbial biodiversity is necessary to assess water ecosystem health and the quality of water resources. We investigated the short-term (2 days) vertical and diel variations in bacterial community structure and microbially derived secondary metabolites during a cyanobacterial bloom that emerged in a highly urbanized tropical reservoir. The waterbody was largely dominated by the cyanobacteria Planktothricoides spp., together with the Synechococcus, Pseudanabaena, Prochlorothrix, and Limnothrix. Spatial differences (i.e., water depth) rather than temporal differences (i.e., day versus night) better-explained the short-term variability in water quality parameters and bacterial community composition. Difference in bacterial structure suggested a resource-driven distribution pattern for the community. We found that the freshwater bacterial community associated with cyanobacterial blooms is largely conserved at the phylum level, with Proteobacteria (β-proteobateria), Bacteroidetes, and Actinobacteria as the main taxa despite the cyanobacterial species present and geographical (Asia, Europe, Australia, and North America) or climatic distinctions. Through multivariate statistical analyses of the bacterial community, environmental parameters, and secondary metabolite concentrations, we observed positive relationships between the occurrences of cyanobacterial groups and off-flavor compounds (2-methyisoborneol and β-ionone), suggesting a cyanobacterial origin. This study demonstrates the potential of 16S rRNA gene amplicon sequencing as a supporting tool in algal bloom monitoring or water-resource management.
authors.affiliation: NUS Environmental Research Institute, National University of Singapore , 5A Engineering Drive 1, No. 02-01 T-Lab Building, Singapore 117411.
authors.forename: Shu Harn
authors.identifier: 0000-0002-7288-6512
authors.initials: SH
authors.lastname: Te
authors.affiliation: Centre for Environmental Sensing and Modelling, Singapore-MIT Alliance for Research and Technology Centre , 1 CREATE Way, #09-03 CREATE Tower, Singapore 138602.
authors.forename: Boon Fei
authors.initials: BF
authors.lastname: Tan
authors.affiliation: Centre for Environmental Sensing and Modelling, Singapore-MIT Alliance for Research and Technology Centre , 1 CREATE Way, #09-03 CREATE Tower, Singapore 138602.|Department of Civil and Environmental Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Janelle R
authors.initials: JR
authors.lastname: Thompson
authors.affiliation: NUS Environmental Research Institute, National University of Singapore , 5A Engineering Drive 1, No. 02-01 T-Lab Building, Singapore 117411.|Department of Civil and Environmental Engineering, National University of Singapore , 1 Engineering Drive 2, E1A 07-03, Singapore 117576.
authors.forename: Karina Yew-Hoong
authors.initials: KY
authors.lastname: Gin
mesh_terms: D000458:Cyanobacteria / Q000145:classification*; D005068:Eutrophication; D005618:Fresh Water / Q000382:microbiology; D064307:Microbiota; D010839:Phytoplankton; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S