title: Susceptibility to Ulcerative Colitis and Genetic Polymorphisms of A251G SOD1 and C-262T CAT.
abstract: BACKGROUND
  Reactive oxygen species can attack and damage almost every molecule found in living cells, including proteins, carbohydrates, lipids, and DNA. For this reason, their production is normally tightly controlled. Among the most important defenses against these radicals are the superoxide dismutase (SOD) enzymes and catalase (CAT). Increasing attention has been given to the role of reactive oxygen metabolites in the pathogenesis of ulcerative colitis (UC), which is defined as an idiopathic and chronic intestinal inflammation. Accordingly, we hypothesized a relation between genetic polymorphisms in the two antioxidant enzymesSOD1A251G (rs2070424) andCATC-262T (rs1001179) and the risk of UC.
  
  
  METHODS
  The present case-control study included 109 UC patients (46 males and 50 females) and 186 (67 males and 119 females) gender-matched healthy controls. Genotyping was done by the PCR-RFLP method.
  
  
  RESULTS
  After adjusting for age and gender, a significant association was observed between the AG+GG genotypes ofSOD1A251G polymorphism (vs. AA genotype) and risk of UC (OR=0.29, 95% CI: 0.10-0.86, P= 0.025) after adjusting for age and gender. Our statistical analysis revealed that theCATC-262T polymorphism did not associate with the risk of UC before and/or after adjusting for age and gender.
  
  
  CONCLUSIONS
  Based on the present statistical analysis, the G allele of theSOD1A251G polymorphism decreases the risk of UC, thus it might be assumed that the G allele has a protective role.
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Gadier
authors.initials: G
authors.lastname: El-Kheshen
authors.affiliation: Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Moeini
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Saadat
keywords: CAT; SOD1; inflammatory bowel disease; ulcerative colitis