title: Construction of 3D multicellular microfluidic chip for an in vitro skin model.
abstract: Current in vitro skin models do not recapitulate the complex architecture and functions of the skin tissue. In particular, on-chip construction of an in vitro model comprising the epidermis and dermis layer with vascular structure for mass transport has not been reported yet. In this study, we aim to develop a microfluidic, three-dimensional (3D) skin chip with fluidic channels using PDMS and hydrogels. Mass transport within the collagen hydrogel matrix was verified with fluorescent model molecules, and a transport-reaction model of oxygen and glucose inside the skin chip was developed to aid the design of the microfluidic skin chip. Comparison of viabilities of dermal fibroblasts and HaCaT cultured in the chip with various culture conditions revealed that the presence of flow plays a crucial role in maintaining the viability, and both cells were viable after 10 days of air exposure culture. Our 3D skin chip with vascular structures can be a valuable in vitro model for reproducing the interaction between different components of the skin tissue, and thus work as a more physiologically realistic platform for testing skin reaction to cosmetic products and drugs.
authors.affiliation: Department of Chemical Engineering, Hongik University, Seoul, Republic of Korea.
authors.forename: Sojin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.|Institute of Human-Environmental Interface Biology, Medical Research Center, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seon-Pil
authors.initials: SP
authors.lastname: Jin
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.|Institute of Human-Environmental Interface Biology, Medical Research Center, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yeon Kyung
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Material Science & Engineering, Hallym University, Chuncheon, Republic of Korea.
authors.forename: Gun Yong
authors.initials: GY
authors.lastname: Sung
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.|Institute of Human-Environmental Interface Biology, Medical Research Center, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
authors.affiliation: Department of Chemical Engineering, Hongik University, Seoul, Republic of Korea. jhsung22@hongik.ac.kr.
authors.forename: Jong Hwan
authors.identifier: 0000-0002-4019-866X
authors.initials: JH
authors.lastname: Sung
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D012867:Skin / Q000166:cytology*
chemical_list: D004129:Dimethylpolysiloxanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C013830:baysilon; D003094:Collagen
keywords: In vitro skin model; Microfluidic; Organ-on-a-chip; Transport phenomena