title: Liposomes Incorporating Transferrin and Stearic Acid-modified Octa-arginine for siRNA Delivery.
abstract: Liposomes incorporating stearic acid-modified octa-arginine (StA-R8) and conjugated to transferrin (Tf) were synthesized and evaluated for delivery of small interfering RNA (siRNA) against survivin. Characteristics, cytotoxicity, cellular uptake and biological activity of liposomes complexed to survivin siRNA were investigated. Tf-conjugated liposomes were shown to have reduced cytotoxicity and improved delivery efficiency for siRNA into cancer cells compared to non-conjugated StA-R8 liposomes. These data suggest that Tf-conjugated StA-R8 liposomes are promising vehicles for siRNA delivery in cancer therapy.
authors.affiliation: College of Animal Science, Jilin University, Changchun, P.R. China.|Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, P.R. China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Jilin University, Changchun, P.R. China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Jilin University, Changchun, P.R. China.|Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
authors.affiliation: College of Animal Science, Jilin University, Changchun, P.R. China xiejing@jlu.edu.cn songcailiu@jluhp.edu.cn.
authors.forename: Songcai
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Jilin University, Changchun, P.R. China xiejing@jlu.edu.cn songcailiu@jluhp.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
mesh_terms: D001120:Arginine / Q000737:chemistry*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D013229:Stearic Acids / Q000737:chemistry*; D000077022:Survivin; D014168:Transferrin / Q000737:chemistry*
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008081:Liposomes; D034741:RNA, Small Interfering; D013229:Stearic Acids; D000077022:Survivin; D014168:Transferrin; C031183:stearic acid; D001120:Arginine
keywords: Cell-penetrating peptide; cancer; liposomes; survivin; transferrin