title: GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.
abstract: Glycyl-l-histidyl-l-lysine (GHK)-Cu is considered to be an activator of tissue remodeling, and has been used in cosmetic products. In this study, we prepared liposomes encapsulating GHK-Cu and analyzed their effect on human umbilical vein endothelial cells (HUVECs) proliferation and scald wound healing in mice. The nanoscaled GHK-Cu-liposomes promoted HUVECs proliferation, with a 33.1% increased rate. Flow cytometry analysis showed increased cell number at G1 stage and decreased cell number at G2 stage after GHK-Cu-liposomes treatment. Western blotting indicated that the expression of vascular endothelial growth factor and fibroblast grow factors-2 were both enhanced, as well as cell cycle-related proteins CDK4 and CyclinD1. In a mice scald model, angiogenesis in burned skin treated with GHK-Cu-liposomes was better compared with free GHK-Cu, and immunofluorescence analysis showed enhanced signal of CD31 and Ki67 in GHK-Cu-liposomes treated mice. Moreover, the wound healing time was shortened to 14 days post injury. Our results provide the evidence that GHK-Cu-liposomes could be utilized as a treatment for skin wounds.
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biotechnology and Bioresources Utilization, (Dalian Minzu University), Ministry of Education, Dalian, China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Liu
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Meijun
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002056:Burns / Q000188:drug therapy* / Q000378:metabolism; D016203:CDC2 Protein Kinase; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D051358:Cyclin-Dependent Kinase 4; D004195:Disease Models, Animal; D005346:Fibroblast Growth Factors; D005347:Fibroblasts; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D008081:Liposomes; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D008081:Liposomes; D009842:Oligopeptides; D042461:Vascular Endothelial Growth Factor A; C020905:glycyl-histidyl-lysine; D005346:Fibroblast Growth Factors; D016203:CDC2 Protein Kinase; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4