title: A novel staphylococcal enterotoxin B subunit vaccine candidate elicits protective immune response in a mouse model.
abstract: Staphylococcal enterotoxin B (SEB), produced by the gram-positive bacterium Staphylococcus aureus, is responsible for food poisoning and toxic shock syndrome, and is considered a potential bioterrorism agent. Unfortunately, still now no approved vaccines are available against SEB. In this study, we constructed a series of nontoxic SEB mutants (mSEBs) and examined whether these mSEBs provide protective immunity against SEB challenge. These mSEB vaccine candidates did not demonstrate superantigen activity in mouse splenocyte cultures. Immunization with the vaccine candidates triggered the production of IgG-antibodies with neutralizing activity. In addition, increased production of IgG1 and IgG3 was observed after immunization, which signifies both Th1- and Th2-induced immune responses. Among the vaccine candidates tested, S9, a double mutant (N23A and Y90A) and S19, a quadruple mutant (N23A, Y90A, R110A, and F177A), demonstrated complete protection against a lethal SEB challenge. Altogether, our results strongly suggest that these mSEBs could be an effective recombinant SEB vaccine candidates for further/future preclinical and clinical studies.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Bio-MAX/N-Bio, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sungho
authors.initials: S
authors.lastname: Shin
authors.affiliation: ABION Inc., R&D Center, Seoul, Republic of Korea.
authors.forename: Na Young
authors.initials: NY
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Pocheon-si, Republic of Korea.
authors.forename: Woo Sung
authors.initials: WS
authors.lastname: Son
authors.affiliation: College of Pharmacy, Sahmyook University, Seoul, Republic of Korea.
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Kang
authors.affiliation: Agency for Defense Development, Republic of Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Song
authors.affiliation: Agency for Defense Development, Republic of Korea.
authors.forename: Chi Ho
authors.initials: CH
authors.lastname: Yu
authors.affiliation: Agency for Defense Development, Republic of Korea.
authors.forename: Gyeung Haeng
authors.initials: GH
authors.lastname: Hur
authors.affiliation: Agency for Defense Development, Republic of Korea.
authors.forename: Seong Tae
authors.initials: ST
authors.lastname: Jeong
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, Republic of Korea; Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea; Bio-MAX/N-Bio, Seoul National University, Seoul, Republic of Korea. Electronic address: ykeeshin@snu.ac.kr.
authors.forename: Young Kee
authors.initials: YK
authors.lastname: Shin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000276:immunology*; D005260:Female; D007114:Immunization; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D011487:Protein Conformation; D012772:Shock, Septic / Q000276:immunology* / Q000517:prevention & control; D013202:Staphylococcal Food Poisoning / Q000276:immunology* / Q000517:prevention & control; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D018089:Superantigens / Q000097:blood; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D004768:Enterotoxins; D013209:Staphylococcal Vaccines; D018089:Superantigens; D022223:Vaccines, Subunit; C031818:enterotoxin B, staphylococcal
keywords: Mutation; Recombinant vaccine; Staphylococcal enterotoxin B; Vaccine candidates