title: A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri.
abstract: Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.
authors.affiliation: Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Kraemer
authors.affiliation: Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Todd A
authors.initials: TA
authors.lastname: Brenner
authors.affiliation: Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Julia O
authors.initials: JO
authors.lastname: Krumholz
authors.affiliation: Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: hrosenberg@niaid.nih.gov.
authors.forename: Helene F
authors.initials: HF
authors.lastname: Rosenberg
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry; D000818:Animals; D004804:Eosinophils / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods*; D006098:Granulocytes / Q000382:microbiology; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology; D007612:Kanamycin / Q000494:pharmacology; D052200:Limosilactobacillus reuteri / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000493:pharmacokinetics*
chemical_list: D000617:Aminoglycosides; D007612:Kanamycin
keywords: Fluorescence; Granulocyte; Inflammation; Probiotic