title: Resveratrol promotes regression of renal carcinoma cells via a renin-angiotensin system suppression-dependent mechanism.
abstract: The aim of the present study was to investigate the effect of resveratrol on renal carcinoma cells and explore possible renin-angiotensin system-associated mechanisms. Subsequent to resveratrol treatment, the cell viability, apoptosis rate, cytotoxicity levels, caspase 3/7 activity and the levels of angiotensin II (AngII), AngII type 1 receptor (AT1R), vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) were evaluated in renal carcinoma cells. The effects of AngII, AT1R, VEGF and COX-2 on resveratrol-induced cell growth inhibition and apoptosis were also examined. The results indicated that resveratrol treatment may suppress growth, induce apoptosis, and decrease AngII, AT1R, VEGF and COX-2 levels in renal carcinoma ACHN and A498 cells. In addition, resveratrol-induced cell growth suppression and apoptosis were reversed when co-culturing with AT1R or VEGF. Thus, resveratrol may suppress renal carcinoma cell proliferation and induce apoptosis via an AT1R/VEGF pathway.
authors.affiliation: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.
authors.forename: Jianchang
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.
authors.forename: Mingning
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.
authors.forename: Lieqian
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.
authors.forename: Guobin
authors.initials: G
authors.lastname: Tan
authors.affiliation: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
keywords: apoptosis; cell proliferation; renal carcinoma cells; renin-angiotensin system; resveratrol