title: Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion.
abstract: Multivalent receptor-mediated interactions between virions and a lipid membrane can be weakened using competitive nonpathogenic ligand binding. In particular, the subsequent binding of such ligands can induce detachment of bound virions, a phenomenon of crucial relevance for the development of new antiviral drugs. Focusing on the simian virus 40 (SV40) and recombinant cholera toxin B subunit (rCTB), and using (monosialotetrahexosyl)ganglioside (GM1) as their common receptor in a supported lipid bilayer (SLB), we present the first detailed investigation of this phenomenon by employing the quartz crystal microbalance with dissipation (QCM-D) and total internal reflection fluorescence (TIRF) microscopy assisted 2D single particle tracking (SPT) techniques. Analysis of the QCM-D-measured release kinetics made it possible to determine the binding strength of a single SV40-GM1 pair. The release dynamics of SV40, monitored by SPT, revealed that a notable fraction of SV40 becomes mobile just before the release, allowing to estimate the distribution of SV40-bound GM1 receptors just prior to release.
authors.affiliation: Department of Physics, Chalmers University of Technology , Gothenburg, Sweden.
authors.forename: Nagma
authors.initials: N
authors.lastname: Parveen
authors.affiliation: Department of Physics, Chalmers University of Technology , Gothenburg, Sweden.
authors.forename: Stephan
authors.initials: S
authors.lastname: Block
authors.affiliation: Department of Physics, Chalmers University of Technology , Gothenburg, Sweden.|Boreskov Institute of Catalysis, Russian Academy of Sciences , Novosibirsk, Russia.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Zhdanov
authors.affiliation: Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg , Gothenburg, Sweden.
authors.forename: Gustaf E
authors.initials: GE
authors.lastname: Rydell
authors.affiliation: Department of Physics, Chalmers University of Technology , Gothenburg, Sweden.
authors.forename: Fredrik
authors.identifier: 0000-0003-1994-5015
authors.initials: F
authors.lastname: Höök
mesh_terms: D000818:Animals; D002417:Cattle; D002772:Cholera Toxin / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry; D013539:Simian virus 40 / Q000378:metabolism; D014771:Virion / Q000378:metabolism*; D053585:Virus Attachment / Q000187:drug effects*
chemical_list: D008024:Ligands; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D005677:G(M1) Ganglioside; D002772:Cholera Toxin; C028694:1-palmitoyl-2-oleoylphosphatidylcholine