title: Honey improves spermatogenesis and hormone secretion in testicular ischaemia-reperfusion-induced injury in rats.
abstract: This study was conducted to survey the protective effect of pre-treatment with Persian honey during post-ischaemia reperfusion on ischaemia-reperfusion (IR)-induced testis injury. Animals were divided into four groups of IR, honey + ischaemia- reperfusion (HIR), vitamin C + ischaemia- reperfusion (VIR) and carbohydrates + ischaemia- reperfusion (CIR). The testes were examined for spermatogenesis index. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed. Total serum concentration of FSH, LH and testosterone was measured using ELISA. All data were expressed as mean ± SD in each group, and significance was set at p ≤ .05. Spermatogenesis index was significant in the HIR group (p < .001). Serum levels of FSH and LH were significantly higher in the CIR and HIR groups. Serum levels of testosterone were significantly higher in VIR and HIR groups. Apoptotic cells in IR and CIR groups increased significantly statistically (p < .001), while in HIR and VIR groups, the number of apoptotic cells decreased and the positive cells of TUNEL staining were detected in spermatocytes and spermatid. The present study indicates that honey decreases the cellular damage and apoptosis during testicular I/R injury, with significant protective effects on reproductive hormone production.
authors.affiliation: Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: M
authors.identifier: http://orcid.org/0000-0003-3719-1659
authors.initials: M
authors.lastname: Gholami
authors.affiliation: Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Abbaszadeh
authors.affiliation: Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Khanipour Khayat
authors.affiliation: Department of Social Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Anbari
authors.affiliation: Department of Social Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Baharvand
authors.affiliation: School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
authors.forename: A M
authors.initials: AM
authors.lastname: Gharravi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000097:blood; D006722:Honey; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control*; D013087:Spermatids / Q000187:drug effects; D013090:Spermatocytes / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000098:blood supply* / Q000187:drug effects; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: honey; ischaemia/reperfusion; spermatogenesis; testis