title: Esculetin Neutralises Cytotoxicity oft-BHP but Not of H2O2on Human Leukaemia NB4 Cells.
abstract: The coumarin esculetin shows antioxidant action on some cell types, both by scavenging ROS and by decreasing ROS production. We have previously demonstrated the induction of apoptosis by esculetin on NB4 human leukaemia cells by an ill-defined mechanism related to ROS levels. In this work, we analyze the effect of the simultaneous treatment with esculetin and two oxidants to observe the early events in the mechanism of esculetin-induced apoptosis. Our results show that, from the early time of 15 min, esculetin acts synergistically with H2O2to decrease cell viability and metabolic activity and to increase apoptosis in NB4 cells. In contrast, the early oxidative effects oft-BHP are neutralised by esculetin, protecting human leukaemia NB4 cells from apoptosis. Esculetin seems to restrict the increase in peroxides caused by H2O2ort-BHP in the time interval analyzed. These results contribute to a better understanding of the cytotoxic effect caused by esculetin on NB4 cells. At the same time, the early neutralisation of exogenous oxidants could be of interest to prevent diseases related to oxidative stress imbalance.
authors.affiliation: Unidad de Bioquímica y Biología Molecular, Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Rubio
authors.affiliation: Unidad de Bioquímica y Biología Molecular, Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: García-Pérez
authors.affiliation: Unidad de Bioquímica y Biología Molecular, Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Tejedor
authors.affiliation: Unidad de Bioquímica y Biología Molecular, Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: Angel
authors.identifier: 0000-0002-9900-6845
authors.initials: A
authors.lastname: Herráez
authors.affiliation: Unidad de Bioquímica y Biología Molecular, Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: José C
authors.identifier: 0000-0002-6435-505X
authors.initials: JC
authors.lastname: Diez
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000633:toxicity; D007938:Leukemia / Q000188:drug therapy; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014468:Umbelliferones / Q000008:administration & dosage* / Q000737:chemistry; D020122:tert-Butylhydroperoxide / Q000737:chemistry / Q000633:toxicity
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014468:Umbelliferones; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; C007628:esculetin