title: Cryobanking Mesenchymal Stem Cells.
abstract: Cryopreservation and storage of culture-expanded mesenchymal stem cells (MSCs) is essential for a biobank to maintain a collection of cell lines for research and clinical use. Optimization of cryopreservation protocols and methods to minimize damage to cells during freezing and thawing is critical to ensure reliable availability of viable cells. Controlling the freezing rate and the use of appropriate cryoprotectant, as well as stable storage temperature, can minimize the negative effects on cell viability. In this chapter, protocols for cryopreserving MSCs are described.
authors.affiliation: StemTek Therapeutics, Derio, 48160, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Pavón
authors.affiliation: StemTek Therapeutics, Derio, 48160, Spain.
authors.forename: Izaskun
authors.initials: I
authors.lastname: Beloqui
authors.affiliation: StemTek Therapeutics, Derio, 48160, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Salcedo
authors.affiliation: StemTek Therapeutics, Derio, 48160, Spain. agmartin@stemtektherapeutics.com.
authors.forename: Angel G
authors.initials: AG
authors.lastname: Martin
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D005615:Freezing; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents
keywords: Control rate freezing; Cryopreservation; Cryoprotectant; DMSO; Mesenchymal stem cells; Viability