title: Electrochemical Characterization of Graphene and MWCNT Screen-Printed Electrodes Modified with AuNPs for Laccase Biosensor Development.
abstract: The aim of this work is to show how the integration of gold nanoparticles (AuNPs) into multi-wall-carbon-nanotubes (MWCNTs) based screen-printed electrodes and into graphene-based screen-printed electrodes (GPHs) could represent a potential way to further enhance the electrochemical properties of those electrodes based on nanoparticles. Laccase fromTrametes versicolor(TvL) was immobilized over MWCNTs and GPH previously modified with AuNPs (of 5 and 10 nm). The characterization of the modified electrode surface has been carried out by cyclic voltammetry. The results showed that the use of AuNPs for modification of both graphene and MWCNTs screen-printed electrode surfaces would increase the electrochemical performances of the electrodes. MWCNTs showed better results than GPH in terms of higher electroactive area formation after modification with AuNPs. The two modified nanostructured electrodes were successively proven to efficiently immobilize the TvL; the electrochemical sensing properties of the GPH- and MWCNT-based AuNPs-TvL biosensors were investigated by choosing 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic-acid diammonium salt (ABTS), catechol and caffeic acid as laccase mediators; and the kinetic parameters of the laccase biosensor were carefully evaluated.
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy. gabriele.favero@uniroma1.it.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy. gabriele.favero@uniroma1.it.|Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy. gabriele.favero@uniroma1.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Fusco
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy. franco.mazzei@uniroma1.it.
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
authors.affiliation: Department of Chemistry of Materials, University of Santiago of Chile, Av. Libertador Bernardo O' Higgins 3363 Estacíon Central, Santiago, Chile. federico.tasca@usach.cl.
authors.forename: Federico
authors.initials: F
authors.lastname: Tasca
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Roma, Italy. riccarda.antiochia@uniroma1.it.
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
keywords: biosensor; gold nanoparticles; graphene; laccase; multi-walled carbon nanotubes; screen-printed electrodes