title: Facile synthesis of bovine serum albumin conjugated low-dimensional ZnS nanocrystals.
abstract: We present a facile synthesis of bovine serum albumin (BSA) conjugated low-dimensional ZnS nanocrystals. The experimental parameters such as effects of BSA concentration and precursor vol ratios of Zn:S on the formation of ZnS nanoparticles in BSA matrix were investigated. The ZnS crystalline sizes of 1.9, 1.8 and 1.6nm were obtained by using the BSA concentrations of 1×10-4, 5×10-4and 10×10-4g/mL, respectively, with a fixed Zn:S vol ratio of 1:1. The ZnS samples prepared from 1:10 and 10:1 vol ratios of Zn:S at BSA concentration of 5×10-4g/mL shows the crystalline sizes of ZnS are 2.1 and 1.5nm, respectively. FT-IR analysis suggests that the prepared ZnS nanoparticles might be conjugated through the interactions of hydroxyl and amine groups present in BSA. We evaluate the cytotoxicity of the prepared ZnS nanoparticles, the THP-1 cells showed a good viability (>88%) for all the prepared ZnS samples. The plausible mechanism for the formation of ZnS-BSA composite has also been discussed.
authors.affiliation: School of Engineering Science and Technology, University of Hyderabad, Gachibowli, Hyderabad, 500046, India.
authors.forename: Bhanu
authors.initials: B
authors.lastname: Mullamuri
authors.affiliation: Department of Engineering Chemistry, SRKR Engineering College, China Amiram, Andhra Pradesh, 534204, India.
authors.forename: Subrahmanya Diwakar
authors.initials: SD
authors.lastname: Bhagavathula
authors.affiliation: School of Engineering Science and Technology, University of Hyderabad, Gachibowli, Hyderabad, 500046, India.
authors.forename: Krishna C S B
authors.initials: KCSB
authors.lastname: Kasturi
authors.affiliation: School of Engineering Science and Technology, University of Hyderabad, Gachibowli, Hyderabad, 500046, India. Electronic address: rvenu8@uohyd.ac.in.
authors.forename: Venu
authors.initials: V
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D060326:Chemistry Techniques, Synthetic; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013440:Sulfides / Q000138:chemical synthesis* / Q000737:chemistry*; D017967:Zinc Compounds / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D013440:Sulfides; D017967:Zinc Compounds; D012710:Serum Albumin, Bovine; C031238:zinc sulfide
keywords: Bioconjugation; Bovine serum albumin; ZnS nanoparticles