title: Quantification of Total Folate, Folate Species and Polyglutamyl Folate Distribution in Winged Beans (Psophocarus tetragonolobus (L) DC) from Different Cultivars and Growth Stages by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry.
abstract: Winged beans are an important natural source of some micronutrients. This paper presents the first complete characterization of folate derivatives including polyglutamyl 5-methyltetrahydrofolate (5-CH3-H4PteGlun), folate species and total folate accumulating in pods and immature seeds of winged beans from 9 cultivars and 7 growth stages. 5-CH3-H4PteGlunand folate species were determined with a UHPLC-MS/MS method. Accurate determination of 5-CH3-H4PteGlunand folate species was optimized and validated according to EMA guidelines including method selectivity, sensitivity, linearity, accuracy, precision, matrix effect and carry-over. The level of total folate is in the range of 73-200 μg/100 g in the pods and 33-61 μg/100 g in the immature seeds. The predominant folate species in winged beans is 5-CH3-H4PteGlu1. 5-CH3-H4PteGlu5is the major polyglutamyl folate derivative. The level of total folate is increased about 4 fold with advancing maturity. For pods, the chain length is increased with growth which shifts from 5-CH3-H4PteGlu1in the early stage to 5-CH3-H4PteGlu5and 5-CH3-H4PteGlu6in the 7th stage. Our findings demonstrate that winged beans are good source of folate. The validated UHPLC-MS/MS method allows for the determination of 5-CH3-H4PteGlunand folate species from other vegetable matrices.
authors.affiliation: Department of Food Science and Technology, Jinan University.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Luo
authors.forename: Hanying
authors.initials: H
authors.lastname: Duan
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zou
authors.forename: Ruixia
authors.initials: R
authors.lastname: Qiu
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005492:Folic Acid / Q000032:analysis*; D027805:Phaseolus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D013763:Tetrahydrofolates / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry
chemical_list: D013763:Tetrahydrofolates; D005492:Folic Acid; C005984:5-methyltetrahydrofolate