title: XTEND: Extending the depth of field in cryo soft X-ray tomography.
abstract: We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Otón
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona, 08290, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Pereiro
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
authors.forename: José J
authors.initials: JJ
authors.lastname: Conesa
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Chichón
authors.affiliation: Centro Nacional de Microbiología, ISCIII, Majadahonda, Madrid, 28220, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Luque
authors.affiliation: Centro Nacional de Microbiología, ISCIII, Majadahonda, Madrid, 28220, Spain.
authors.forename: Javier M
authors.initials: JM
authors.lastname: Rodríguez
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona, 08290, Spain.
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Pérez-Berná
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
authors.forename: Carlos Oscar S
authors.initials: CO
authors.lastname: Sorzano
authors.affiliation: Department of Computer Science, The Graduate Center, City University of New York, NY 10016, USA.
authors.forename: Joanna
authors.initials: J
authors.lastname: Klukowska
authors.affiliation: Department of Computer Science, The Graduate Center, City University of New York, NY 10016, USA.
authors.forename: Gabor T
authors.initials: GT
authors.lastname: Herman
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Vargas
authors.affiliation: Escuela Politécnica Superior, Univ. Autónoma de Madrid, Cantoblanco, Madrid, 28049, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Marabini
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.|Unidad Asociada CNB-Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia),Cantoblanco, Madrid, 28049, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Carrascosa
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Carazo
mesh_terms: D000465:Algorithms; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D014056:Tomography, X-Ray / Q000379:methods*