title: DNA/Ag Nanoparticles as Antibacterial Agents against Gram-Negative Bacteria.
abstract: Silver (Ag) nanoparticles were produced using DNA extracted from salmon milt as templates. Particles spherical in shape with an average diameter smaller than 10 nm were obtained. The nanoparticles consisted of Ag as the core with an outermost thin layer of DNA. The DNA/Ag hybrid nanoparticles were immobilized over the surface of cotton based fabrics and their antibacterial efficiency was evaluated usingE. colias the typical Gram-negative bacteria. The antibacterial experiments were performed according to the Antibacterial Standard of Japanese Association for the Functional Evaluation of Textiles. The fabrics modified with DNA/Ag nanoparticles showed a high enough inhibitory and killing efficiency againstE. coliat a concentration of Ag ≥ 10 ppm.
authors.affiliation: Graduate School of Environmental Science, Hokkaido University, Hokkaido 060-0810, Japan. tomomi.takeshima@utsouthwestern.edu.|Nissei Bio Co. Ltd., Hokkaido 061-1374, Japan. tomomi.takeshima@utsouthwestern.edu.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takeshima
authors.affiliation: Nissei Bio Co. Ltd., Hokkaido 061-1374, Japan. tada@nisseibio.co.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Tada
authors.affiliation: Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Hokkaido 060-8628, Japan. sakaguchi@eng.hokudai.ac.jp.
authors.forename: Norihito
authors.initials: N
authors.lastname: Sakaguchi
authors.affiliation: Graduate School of Dental Medicine, Hokkaido University, Hokkaido 060-8586, Japan. watari@den.hokudai.ac.jp.
authors.forename: Fumio
authors.initials: F
authors.lastname: Watari
authors.affiliation: Graduate School of Environmental Science, Hokkaido University, Hokkaido 060-0810, Japan. bunshifugetsu@pari.u-tokyo.ac.jp.|Policy Alternative Research Institute, The University of Tokyo, Tokyo 113-0032, Japan. bunshifugetsu@pari.u-tokyo.ac.jp.
authors.forename: Bunshi
authors.initials: B
authors.lastname: Fugetsu
keywords: DNA/Ag nanoparticles; Gram-negative bacteria; antibacterial; electrostatic interaction; nanoparticle immobilization; salmon milt DNA