title: Preparation and characterization of aminoethyl hydroxypropyl starch modified with collagen peptide.
abstract: The preparation of aminoethyl hydroxypropyl starch collagen peptide (AEHPS-COP) was via an enzyme-catalyzed reaction between amino groups in aminoethyl hydroxypropyl starch (AEHPS) and γ-carboxamide groups in collagen peptide (COP) by using microbial transglutaminase (MTGase) as biocatalyst. As an intermediate reactant, AEHPS was synthesized from hydroxypropyl starch (HPS) and 2-chloroethylamine hydrochloride (CEH). The chemical structures of HPS, AEHPS and AEHPS-COP were characterized by Fourier transform infrared spectroscopy (FT-IR) and13C nuclear magnetic resonance (13C NMR). The reaction conditions that influenced the degree of substitution (DS) of AEHPS-COP were optimized, which included the reaction temperature, the reaction time, the mass ratio of collagen peptide to aminoethyl hydroxypropyl starch and the pH value. In addition, in vitro antioxidant activities of AEHPS-COP were evaluated through the scavenging activity of hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Furthermore, the methylthiazol tetrazolium (MTT) assay was applied to investigate the cell viability of AEHPS-COP. The results indicated that the AEHPS-COP exhibited better cell viability to L929 mouse fibroblast cells. Therefore, the AEHPS-COP showed a promising potential application in cosmetic, biomedical and pharmaceutical fields.
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Huigao
authors.initials: H
authors.lastname: Wen
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Ge
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Shengqiong
authors.initials: S
authors.lastname: Zou
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
authors.forename: Han
authors.initials: H
authors.lastname: Feng
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China. Electronic address: lihongfan2000@hotmail.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D005021:Ethylamines / Q000737:chemistry; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D010446:Peptide Fragments / Q000737:chemistry*; D051381:Rats; D013213:Starch / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity
chemical_list: D005021:Ethylamines; D016166:Free Radical Scavengers; D010446:Peptide Fragments; D013213:Starch; D003094:Collagen; C046042:2-hydroxypropyl starch; C010076:2-chloroethylamine
keywords: Aminoethyl hydroxypropyl starch; Antioxidant activity; Cell viability; Collagen peptide