title: The extent of the temperature-induced membrane remodeling in two closely relatedBordetellaspecies reflects their adaptation to diverse environmental niches.
abstract: Changes in environmental temperature represent one of the major stresses faced by microorganisms as they affect the function of the cytoplasmic membrane. In this study, we have analyzed the thermal adaptation in two closely related respiratory pathogensBordetella pertussisandBordetella bronchisepticaAlthoughB. pertussisrepresents a pathogen strictly adapted to the human body temperature,B. bronchisepticacauses infection in a broad range of animals and survives also outside of the host. We applied GC-MS to determine the fatty acids of bothBordetellaspecies grown at different temperatures and analyzed the membrane fluidity by fluorescence anisotropy measurement. In parallel, we also monitored the effect of growth temperature changes on the expression and production of several virulence factors. In response to low temperatures,B. pertussisadapted its fatty acid composition and membrane fluidity to a considerably lesser extent when compared withB. bronchisepticaRemarkably,B. pertussismaintained the production of virulence factors at 24 °C, whereasB. bronchisepticacells resumed the production only upon temperature upshift to 37 °C. This growth temperature-associated differential modulation of virulence factor production was linked to the phosphorylation state of transcriptional regulator BvgA. The observed differences in low-temperature adaptation betweenB. pertussisandB. bronchisepticamay result from selective adaptation ofB. pertussisto the human host. We propose that the reduced plasticity of theB. pertussismembranes ensures sustained production of virulence factors at suboptimal temperatures and may play an important role in the transmission of the disease.
authors.affiliation: From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Seydlova
authors.affiliation: From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Beranova
authors.affiliation: the Laboratories of Post-transcriptional Control of Gene Expression and.
authors.forename: Ilona
authors.initials: I
authors.lastname: Bibova
authors.affiliation: the Laboratories of Post-transcriptional Control of Gene Expression and.
authors.forename: Ana
authors.initials: A
authors.lastname: Dienstbier
authors.affiliation: the Laboratories of Post-transcriptional Control of Gene Expression and.
authors.forename: Jakub
authors.initials: J
authors.lastname: Drzmisek
authors.affiliation: Molecular Biology of Bacterial Pathogens, Institute of Microbiology v.v.i., Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Masin
authors.affiliation: From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.
authors.forename: Radovan
authors.initials: R
authors.lastname: Fiser
authors.affiliation: From the Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 12843 Prague, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Konopasek
authors.affiliation: the Laboratories of Post-transcriptional Control of Gene Expression and vecerek@biomed.cas.cz.
authors.forename: Branislav
authors.initials: B
authors.lastname: Vecerek
mesh_terms: D000064:Acclimatization; D016880:Anisotropy; D001426:Bacterial Proteins / Q000378:metabolism; D001831:Body Temperature; D016950:Bordetella bronchiseptica / Q000166:cytology* / Q000502:physiology; D001886:Bordetella pertussis / Q000166:cytology* / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004777:Environment; D005227:Fatty Acids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010766:Phosphorylation; D015398:Signal Transduction; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D005227:Fatty Acids; D014157:Transcription Factors; D037521:Virulence Factors
keywords: bacterial pathogenesis; bacterial signal transduction; fatty acid; host adaptation; membrane function; virulence factor