title: Investigate the Effect of Thawing Process on the Self-Assembly of Silk Protein for Tissue Applications.
abstract: Biological self-assembly is a process in which building blocks autonomously organize to form stable supermolecules of higher order and complexity through domination of weak, noncovalent interactions. For silk protein, the effect of high incubating temperature on the induction of secondary structure and self-assembly was well investigated. However, the effect of freezing and thawing on silk solution has not been studied. The present work aimed to investigate a new all-aqueous process to form 3D porous silk fibroin matrices using a freezing-assisted self-assembly method. This study proposes an experimental investigation and optimization of environmental parameters for the self-assembly process such as freezing temperature, thawing process, and concentration of silk solution. The optical images demonstrated the possibility and potential of -80ST48 treatment to initialize the self-assembly of silk fibroin as well as controllably fabricate a porous scaffold. Moreover, the micrograph images illustrate the assembly of silk protein chain in 7 days under the treatment of -80ST48 process. The surface morphology characterization proved that this method could control the pore size of porous scaffolds by control of the concentration of silk solution. The animal test showed the support of silk scaffold for cell adhesion and proliferation, as well as the cell migration process in the 3D implantable scaffold.
authors.affiliation: Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University-Vietnam National University-Ho Chi Minh City (VNU-HCM), Ho Chi Minh City 700000, Vietnam.
authors.forename: Hiep Thi
authors.identifier: 0000-0002-1636-7152
authors.initials: HT
authors.lastname: Nguyen
authors.affiliation: Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University-Vietnam National University-Ho Chi Minh City (VNU-HCM), Ho Chi Minh City 700000, Vietnam.
authors.forename: Hien Thu
authors.identifier: 0000-0001-5890-3059
authors.initials: HT
authors.lastname: Luong
authors.affiliation: Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 70000, Vietnam.
authors.forename: Hai Dai
authors.identifier: 0000-0003-3501-7390
authors.initials: HD
authors.lastname: Nguyen
authors.affiliation: Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University-Vietnam National University-Ho Chi Minh City (VNU-HCM), Ho Chi Minh City 700000, Vietnam.
authors.forename: Hien Anh
authors.initials: HA
authors.lastname: Tran
authors.affiliation: Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University-Vietnam National University-Ho Chi Minh City (VNU-HCM), Ho Chi Minh City 700000, Vietnam.
authors.forename: Khon Chan
authors.initials: KC
authors.lastname: Huynh
authors.affiliation: Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University-Vietnam National University-Ho Chi Minh City (VNU-HCM), Ho Chi Minh City 700000, Vietnam.
authors.forename: Toi Van
authors.initials: TV
authors.lastname: Vo
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry* / Q000627:therapeutic use; D005615:Freezing; D006801:Humans; D017433:Protein Structure, Secondary; D047011:Silk / Q000737:chemistry* / Q000627:therapeutic use; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D047011:Silk; D005349:Fibroins