title: Endometrial cancer and microsatellite instability status.
abstract: Microsatellite instability (MSI) is an important factor in the development of various cancers as an identifier of a defective DNA mismatch repair system. The objective of our study was to define the association between microsatellite instability status and traditional clinicopathologic characteristics of endometrioid type adenocarcinoma.
  
  
  METHODS
  MSI status of endometrial cancer was examined by employing the Promega MSI Analysis System. This system uses 5 mononucleotide markers to identify MSI in tumour and normal tissue DNA (BAT-25, BAT-26, NR-21, NR-24, and MONO-27), and 2 pentanucleotide markers (Penta C and Penta D) for specimen identification. In this study, we investigated MSI status in 109 endometrial carcinomas.
  
  
  CONCLUSIONS
  One hundred (92%) of 109 endometrial cancers showed endometrioid type histology and only 9 (8%) non-endometrioid type. MSI-high was found in 17% (17/100) of endometrioid type adenocarcinomas, in 0% (0/9) of non-endometrioid carcinomas. Selected clinicopathologic parameters for endometrioid type adenocarcinomas were compared to the MSI status which was separated into two groups - MSI-high and MSI stable. The results showed that MSI-high status was related to clinicopathologic parameters such as deep myometrial invasion and higher histologic grade in endometrioid type adenocarcinomas.
authors.affiliation: Out Patient Clinic, National cancer institute, Santariskiu 1, LT-08660, Vilnius, Lithuania.
authors.forename: Daiva
authors.initials: D
authors.lastname: Kanopiene
authors.affiliation: Promega Corporation, Madison WI, USA.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Vidugiriene
authors.affiliation: Radiotherapy and Drug Therapy Center, National cancer institute, Santariskiu 1, LT-08660, Vilnius, Lithuania.
authors.forename: Konstantinas Povilas
authors.initials: KP
authors.lastname: Valuckas
authors.affiliation: Scientific Research Center, National cancer institute, Santariskiu 1, LT-08660, Vilnius, Lithuania.
authors.forename: Giedre
authors.initials: G
authors.lastname: Smailyte
authors.affiliation: Scientific Research Center, National cancer institute, Santariskiu 1, LT-08660, Vilnius, Lithuania.
authors.forename: Saule
authors.initials: S
authors.lastname: Uleckiene
authors.affiliation: Promega Corporation, Madison WI, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Bacher
keywords: Clinicopathologic parameters; Endometrial cancer; Microsatellite instability