title: A sensitive liquid chromatography-mass spectrometry method for the simultaneous determination in plasma of pentacyclic triterpenes of Olea europaea L.
abstract: Table olives are especially rich in pentacyclic triterpenic compounds, which exert several biological activities. A crucial step in order to know if these compounds could contribute to the beneficial and healthy properties of this food is their measurement in blood. Therefore, the present study describes a simple and accurate liquid-liquid extraction followed by LC-QqQ-MS analysis for the simultaneous determination of the main pentacyclic triterpenes from Olea europaea L. in rat plasma. The method was validated by the analysis of blank plasma samples spiked with pure compounds, obtaining a linear correlation, adequate sensitivity with a limit of quantification ranging from 1nM for maslinic acid to 10nM for uvaol. Precision and accuracy were lower than 10% in all cases and recoveries were between 95 and 104%. The oral administration of olives to rats and its determination in plasma verified that the established methodology is appropriate for bioavailability studies.
authors.affiliation: Departament de Enginyeria Química i Química Analítica, Universitat de Barcelona (UB), Av. Diagonal 645, 08028 Barcelona, Spain; Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Recinte Torribera, Av. Prat de la Riba 171, 08921 Santa Coloma Gramenet, Spain. Electronic address: estelagimenez@ub.edu.
authors.forename: Estela
authors.initials: E
authors.lastname: Giménez
authors.affiliation: Departament de Bioquímica i Fisiologia, Universitat de Barcelona (UB), Av. Joan XXIII 27-31, 08028 Barcelona, Spain; Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Recinte Torribera, Av. Prat de la Riba 171, 08921 Santa Coloma Gramenet, Spain. Electronic address: scalvomelia@hotmail.com.
authors.forename: M Emília
authors.initials: ME
authors.lastname: Juan
authors.affiliation: Departament de Enginyeria Química i Química Analítica, Universitat de Barcelona (UB), Av. Diagonal 645, 08028 Barcelona, Spain; Departament de Bioquímica i Fisiologia, Universitat de Barcelona (UB), Av. Joan XXIII 27-31, 08028 Barcelona, Spain. Electronic address: mejuan@ub.edu.
authors.forename: Sara
authors.initials: S
authors.lastname: Calvo-Melià
authors.affiliation: Departament de Bioquímica i Fisiologia, Universitat de Barcelona (UB), Av. Joan XXIII 27-31, 08028 Barcelona, Spain; Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Recinte Torribera, Av. Prat de la Riba 171, 08921 Santa Coloma Gramenet, Spain. Electronic address: jmplanas@ub.edu.
authors.forename: Joana M
authors.initials: JM
authors.lastname: Planas
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D059625:Liquid-Liquid Extraction; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D031658:Olea / Q000737:chemistry; D053978:Pentacyclic Triterpenes / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D053978:Pentacyclic Triterpenes
keywords: Erythrodiol (PubChem CID: 101761); Liquid chromatography; Maslinic acid (PubChem CID: 73659); Mass spectrometry; Oleanolic acid (PubChem CID: 10494); Pentacyclic triterpenes; Plasma; Table olives; Ursolic acid (PubChem CID: 64945); Uvaol (PubChem CID: 92802)