title: One step salting-out assisted liquid-liquid extraction followed by UHPLC-ESI-MS/MS for the analysis of isoflavones in soy milk.
abstract: We developed and validated a fast UHPLC-ESI-MS/MS in combination with one step salting-out assisted liquid-liquid extraction (SALLE) for high throughput analysis of 12 isoflavones in soy milks. Conventional sample preparation requires two steps composing time-consuming freeze-drying (72h) and solvent extraction (2h). The SALLE required only about 40min, which was over 100times faster without compromising the extraction efficiency. The baseline separation of 12 isoflavones was achieved in 2.5min by the UHPLC-MS/MS which is the fastest chromatographic method ever reported. The new method was fast, simple, selective, sensitive, precise and accurate. The method was successfully applied to 22 commercial soy milks for the high throughput analysis of isoflavones. The new method would be a fast, simple, reliable, and useful measure for the analysis of isoflavones in soy milk especially in the laboratories facing the challenges of saving the analytical time, labor cost and solvent usage.
authors.affiliation: Department of Food Science and Biotechnology, Graduate School, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-gun, Jeonbuk Province 565-701, Republic of Korea.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Graduate School, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-gun, Jeonbuk Province 565-701, Republic of Korea. Electronic address: munjung@woosuk.ac.kr.
authors.forename: Mun Yhung
authors.initials: MY
authors.lastname: Jung
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007529:Isoflavones / Q000032:analysis*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D012965:Sodium Chloride / Q000737:chemistry*; D041942:Soy Milk / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007529:Isoflavones; D012965:Sodium Chloride
keywords: High throughput analysis; Isoflavones; Salting-out assisted extraction; Soy milk; Tandem mass spectrometry; Ultra-high performance liquid chromatography