title: Circadian Rhythm Influences the Promoting Role of Pulsed Electromagnetic Fields on Sciatic Nerve Regeneration in Rats.
abstract: Circadian rhythm (CR) plays a critical role in the treatment of several diseases. However, the role of CR in the treatment of peripheral nerve defects has not been studied. It is also known that the pulsed electromagnetic fields (PEMF) can provide a beneficial microenvironment to quicken the process of nerve regeneration and to enhance the quality of reconstruction. In this study, we evaluate the impact of CR on the promoting effect of PEMF on peripheral nerve regeneration in rats. We used the self-made "collagen-chitosan" nerve conduits to bridge the 15-mm nerve gaps in Sprague-Dawley rats. Our results show that PEMF stimulation at daytime (DPEMF) has most effective outcome on nerve regeneration and rats with DPEMF treatment achieve quickly functional recovery after 12 weeks. These findings indicate that CR is an important factor that determines the promoting effect of PEMF on peripheral nerve regeneration. PEMF exposure in the daytime enhances the functional recovery of rats. Our study provides a helpful guideline for the effective use of PEMF mediations experimentally and clinically.
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, 323rd Hospital of PLA, Xi'an, China; Department of Anatomy, The Fourth Military Medical University, Xi'an, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, 161st Hospital of PLA , Wuhan , China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Faculty of Biomedical Engineering, Fourth Military Medical University , Xi'an , China.
authors.forename: Da
authors.initials: D
authors.lastname: Jing
authors.affiliation: Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Ran
authors.affiliation: General Political Department Hospital of PLA , Beijing , China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University , Xi'an , China.
authors.forename: Zhuojing
authors.initials: Z
authors.lastname: Luo
keywords: chronotherapy; circadian rhythm; functional recovery; nerve regeneration; peripheral nerves; pulsed electromagnetic fields