title: A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule.
abstract: Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.
authors.affiliation: Department of Cellular and Molecular Function Analysis, Kanazawa University Graduate School of Medical Science, Kanazawa, 920-8640, Japan.
authors.forename: Jun-Ichi
authors.initials: JI
authors.lastname: Morishige
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Ryouhei
authors.initials: R
authors.lastname: Yamashita
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan. tanaka.tamotsu@tokushima-u.ac.jp.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Applied Biological Science, Fukuyama University, Fukuyama, 729-0292, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Satouchi
mesh_terms: D002518:Ceramides / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D006801:Humans; D008246:Lysophospholipids / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000378:metabolism
chemical_list: D002518:Ceramides; D008246:Lysophospholipids; C065576:ceramide 1-phosphate; C060506:sphingosine 1-phosphate; D013110:Sphingosine
keywords: Ceramide-1-phosphate; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Phos-tag; Sphingosine-1-phosphate