title: Discrimination of Nosiheptide Sources with Plasmonic Filters.
abstract: Bacteria identification plays a vital role in the field of clinical diagnosis, food industry, and environmental monitoring, which is in great demand of point of care detection methods. In this paper, in order to discriminate the source of nosiheptide product, a plasmonic filter was fabricated to filtrate, capture and identify Streptomycete spores with Surface enhanced Raman Scattering (SERS). Since the plasmonic filter was derived from self-assembled photonic crystal coated with silver, the plasmonic "hot spots" on the filter surface was distributed evenly in a fare good density and the SERS enhancement factor was 7.49 × 107. With this filter, a stain- and PCR-free detection was realized with only 5 μL sample solution and 5 min in a manner of "filtration and measure". Comparison to traditional Gram stain method and silver-plated nylon filter membrane, the plasmonic filter showed good sensitivity and efficiency in the discrimination of nosiheptide prepared with chemical and biological methods. It is anticipated that this simple SERS detection method with plasmonic filter has promising potentials in food safety, environmental, or clinical applications.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.|Suzhou Key Laboratory of Environment and Biosafety, Suzhou Research Institute of Southeast University , Suzhou 215123, China.
authors.forename: Delong
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.|Suzhou Key Laboratory of Environment and Biosafety, Suzhou Research Institute of Southeast University , Suzhou 215123, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Ni
authors.affiliation: SUNNY GROUP·SEL BIOCHEM , Paradise Software Park, Hangzhou 310012, China.
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.|Suzhou Key Laboratory of Environment and Biosafety, Suzhou Research Institute of Southeast University , Suzhou 215123, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Chemistry, Nanjing University , Nanjing 210093, China.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.|Suzhou Key Laboratory of Environment and Biosafety, Suzhou Research Institute of Southeast University , Suzhou 215123, China.
authors.forename: Zhongze
authors.identifier: http://orcid.org/0000-0001-8926-7710
authors.initials: Z
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.|Suzhou Key Laboratory of Environment and Biosafety, Suzhou Research Institute of Southeast University , Suzhou 215123, China.
authors.forename: Xiangwei
authors.identifier: http://orcid.org/0000-0003-0678-5219
authors.initials: X
authors.lastname: Zhao
mesh_terms: D005374:Filtration; D012834:Silver; D013059:Spectrum Analysis, Raman; D013844:Thiazoles / Q000737:chemistry
chemical_list: D013844:Thiazoles; D012834:Silver; C014134:nosiheptide
keywords: SERS; nosiheptide; photonic crystal; plasmonic filters; spore detection