title: Comparison of Recombinant Proteins of Kinesin 39, Heat Shock Protein 70, Heat Shock Protein 83, and Glycoprotein 63 for Antibody Detection of Leishmania martiniquensis Infection.
abstract: Leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (CL) and visceral leishmaniasis (VL) among humans and animals. This organism, first reported in Martinique Island, now has become an emerging infectious agent in Thailand. Symptomatic cases of L. martiniquensis infection among humans have continuously increased. In the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and control disease transmission. The unsuccessful serological test using the commercial rK39 dipstick based on antigen from Leishmania donovani to detect the antibodies against VL among infected Thai patients has encouraged us to further explore a new sensitive and specific antigenic epitope. In this study, we determined the sequences and expressed recombinant proteins of kinesin 39 (k39), heat shock protein 70 (hsp70), heat shock protein 83 (hsp83), and glycoprotein 63 (gp63) of L. martiniquensis to evaluate the diagnostic efficiency to detect antibodies against L. martiniquensis in patient sera. The preliminary results from western blot analysis have suggested that K39 is the most sensitive recombinant protein to detect L. martiniquensis. Moreover, this recombinant protein reacts with antibodies against L. donovani and Leishmania infantum, making it a promising antigen for further development of a universal rapid diagnostic tool for VL.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Suradej
authors.initials: S
authors.lastname: Siripattanapipong
authors.affiliation: Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, 329-0498, Japan.
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Kato
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Peerapan
authors.initials: P
authors.lastname: Tan-Ariya
authors.affiliation: Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, 10400, Thailand.
authors.forename: Mathirut
authors.initials: M
authors.lastname: Mungthin
authors.affiliation: Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, 10400, Thailand.
authors.forename: Saovanee
authors.identifier: 0000-0003-2696-2075
authors.initials: S
authors.lastname: Leelayoova
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007896:Leishmaniasis / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013785:Thailand
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D011994:Recombinant Proteins
keywords: Visceral leishmaniasis