title: Combined effects off indomethacin and oxaliplatin on lymph node metastasis related factors in human lung cancerxenografts in nude mice.
abstract: To investigate the combined effects of indomethacin and oxaliplatin on expressions of epidermal growth factor receptor (EGFR), E-cadherin (E-cad), intercellular adhesion molecule-1 (ICAM-1) and CD44v6 related to lymph node metastasis of human lung cancer cell lines. Human lung adenocarcinoma A549 cells were inoculated subcutaneously into the left armpit of nude mice to establish human lung cancer xenografts. The mice were randomly divided into control group, indomethacin group, oxaliplatin group and combination therapy group, which were treated with sterile distilled water, indomethacin, oxaliplatin and indomethacin combined with oxaliplatin, respectively. After 42 days, the mice were sacrificed. The immunohistochemistry and reverse transcription polymerase chain reaction were used to detect the expressions of EGFR, E-cad, ICAM-1 and CD44v6 in tumor tissues. Compared to control group, the protein and mRNA expressions of EGFR, ICAM-1 and CD44v6 in the indomethacin, oxaliplatin, and combination therapy groups were significantly reduced (P<0.05) and the protein and mRNA expressions of E-cad expression were significantly increased (P<0.05). Compared to indomethacin group and oxaliplatin group, the protein and mRNA expressions of EGFR, ICAM-1 and CD44v6 in combination therapy groups were significantly reduced (P<0.05), and the protein and mRNA expressions of E-cad expression were significantly increased (P<0.05). There was no significant difference between indomethacin and oxaliplatin groups. Indomethacin and oxaliplatin have synergistic effect on expressions of lymph node metastasis related factors in lung cancer cell lines.
authors.affiliation: Department of Respiratory Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xing
authors.affiliation: Department of Respiratory Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Dong-Chang
authors.initials: DC
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D000077192:Adenocarcinoma of Lung; D000818:Animals; D015703:Antigens, CD; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D004357:Drug Synergism; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D007213:Indomethacin / Q000008:administration & dosage*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009944:Organoplatinum Compounds / Q000008:administration & dosage*; D000077150:Oxaliplatin; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D015703:Antigens, CD; C112697:CD44v6 antigen; C513384:CDH1 protein, human; D015820:Cadherins; D018960:Hyaluronan Receptors; C573420:ICAM1 protein, human; D009944:Organoplatinum Compounds; D012333:RNA, Messenger; D000077150:Oxaliplatin; D018799:Intercellular Adhesion Molecule-1; C512478:EGFR protein, human; D066246:ErbB Receptors; D007213:Indomethacin