title: Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking.
abstract: Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.
authors.affiliation: Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, UK. bgw093@bham.ac.uk.
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Wiggins
authors.affiliation: Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, UK.
authors.forename: Zania
authors.initials: Z
authors.lastname: Stamataki
authors.affiliation: Centre for Liver Research, Immunity and Immunotherapy, Institute of Biomedical Research, University of Birmingham, Birmingham, UK.
authors.forename: Patricia F
authors.initials: PF
authors.lastname: Lalor
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D018925:Chemokines / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods
chemical_list: D015815:Cell Adhesion Molecules; D018925:Chemokines
keywords: Antibody; Human; Inflammation; Liver; Lymphocyte; Organ culture