title: A novel aptasensor for lysozyme based on electrogenerated chemiluminescence resonance energy transfer between luminol and silicon quantum dots.
abstract: In the present work, electrogenerated chemiluminescence (ECL) of luminol was investigated in neutral condition at a gold electrode in the presence of silicon quantum dots (SiQDs). The results revealed that SiQDs can not only greatly enhance luminol ECL, but also act as energy acceptor to construct a novel ECL resonance energy transfer (ECL-RET) system with luminol. As a result, strong anodic ECL signal was obtained in neutral condition at the bare gold electrode, which is suitable for biosensing application. Lysozyme exhibited apparent inhibiting effect on the ECL-RET system, based on which an ECL aptasensor was fabricated for the sensitive detection of lysozyme. The proposed method showed high sensitivity, good selectivity, and wide linearity for the detection of lysozyme in the range of 5.0×10-14-5.0×10-9gmL-1with a detection limit of 5.8×10-15gmL-1(3σ). The results suggested that as-proposed luminol/SiQDs ECL biosensor will be promising in the detection enzyme.
authors.affiliation: School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Dong
authors.affiliation: School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: jjzhu@nju.edu.cn.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004735:Energy Transfer; D006046:Gold / Q000737:chemistry; D006801:Humans; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D045663:Quantum Dots / Q000737:chemistry; D012825:Silicon / Q000737:chemistry
chemical_list: D052158:Aptamers, Peptide; D008165:Luminol; D006046:Gold; D009113:Muramidase; D012825:Silicon
keywords: Electrogenerated chemiluminescence; Luminol; Lysozyme; Resonance energy transfer; Si quantum dots