title: Fluorous Solid-Phase Extraction Technique Based on Nanographite Fluoride.
abstract: Fluorous solid-phase extraction (FSPE) has been employed to isolate target compounds from complex chemical or biological samples in many research areas. However, the lack of efficient and economical available perfluorinated materials impeded its development. In this study, we present a novel nanographite fluoride-based fluorous solid-phase extraction (GF-FSPE) as a replacement of commercially available cartridge-based FSPE, which showed remarkable selectivity, low LOD, high post enrichment recovery, and large enrichment capacity. To demonstrate the potency of GF-FSPE, it was designed and successfully applied to isolate cysteine-containing peptide subsets from complex protein samples to improve the proteome coverage. Additionally, since graphite fluoride was inexpensive and highly commercialized, this study was expected to promote the popularization of FSPE in both chemical and biological separations as well as encourage the synthesis and broaden the application of highly fluorinated carbon fluoride in material science.
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.|Department of Chemistry, Fudan University , Shanghai, 200433, P. R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.|Department of Chemistry, Fudan University , Shanghai, 200433, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.|Department of Chemistry, Fudan University , Shanghai, 200433, P. R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.|Department of Chemistry, Fudan University , Shanghai, 200433, P. R. China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University , Shanghai, 200032, P. R. China.|Department of Chemistry, Fudan University , Shanghai, 200433, P. R. China.|Key Laboratory of Glycoconjugates Research Ministry of Public Health, Fudan University , Shanghai, 200032, P. R. China.
authors.forename: Haojie
authors.identifier: 0000-0003-3477-7662
authors.initials: H
authors.lastname: Lu