title: Development and validation of a fast SFC method for the analysis of flavonoids in plant extracts.
abstract: Flavonoids from plants always show a wide range of biological activities. In the present study, a rapid and highly efficient supercritical fluid chromatography (SFC) method was developed for the separation of 12 flavonoids. After careful optimization, the 12 flavonoids were baseline separated on a ZORBAX RX-SIL column using gradient elution. A 0.1% phosphoric acid solution in methanol was found to be the most suitable polar mobile phase component for the separation of flavonoids. From the viewpoint of retention and resolution, a backpressure of 200bar and a temperature of 40°C were shown to give the best results. Compared with a previously developed reverse phase liquid chromatography method, the SFC method could provide flavonoid separations that were about three times faster, while maintaining good peak shape and comparable peak efficiency. This SFC method was validated and applied to the analysis of five flavonoids (kaempferol, luteolin, quercetin, luteoloside, buddleoside) present in Chrysanthemum morifolium Ramat. from different cultivars (Chuju, Gongju, Hangju, Boju). The results indicated a good repeatability and sensitivity for the quantification of the five analytes with RSDs for overall precision lower than 3%. The limits of detection ranged from 0.73 to 2.34μg/mL, while the limits of quantification were between 2.19 and 5.86μg/mL. The method showed that SFC could be employed as a useful tool for the quality assessment of Traditional Chinese medicines (TCMs) containing flavonoids as active components.
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China; Laboratory for the Analysis of Medicine, Department of Pharmaceutical Sciences, CIRM, University of Liege, CHU B36, B-4000 Liege, Belgium.
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China.
authors.forename: Guangyun
authors.initials: G
authors.lastname: Tang
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China.
authors.forename: Minyi
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China. Electronic address: sunrat_115@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China; Laboratory for the Analysis of Medicine, Department of Pharmaceutical Sciences, CIRM, University of Liege, CHU B36, B-4000 Liege, Belgium.
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China; Laboratory for the Analysis of Medicine, Department of Pharmaceutical Sciences, CIRM, University of Liege, CHU B36, B-4000 Liege, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, Guangdong, 510632, China. Electronic address: jzjjackson@hotmail.com.
authors.forename: Zhengjin
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D025924:Chromatography, Supercritical Fluid; D005419:Flavonoids; D000432:Methanol; D010936:Plant Extracts
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D000432:Methanol
keywords: Flavonoids; Supercritical fluid chromatography; Traditional Chinese medicines