title: Inhibition factors and Kinetic model for ammonium inhibition on the anammox process of the SNAD biofilm.
abstract: The aim of the present work was to evaluate the anaerobic ammonium oxidation (anammox) activity of simultaneous partial nitrification, anammox and denitrification (SNAD) biofilm with different substrate concentrations and pH values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH4+-N removal rate of anammox process remained 87.4% of the maximum rate with the NO2--N concentration of 100mg/L. Based on the results of Haldane model, no obvious difference in kinetic coefficients was observed under high or low free ammonia (FA) conditions, indicating that ammonium rather than FA was the true inhibitor for anammox process of SNAD biofilm. With the pH value of 7.0, the rmax, Ks and KIof ammonium were 0.209kg NO2--N/kg VSS/day, 9.5mg/L and 422mg/L, respectively. The suitable pH ranges for anammox process were 5.0 to 9.0. These results indicate that the SNAD biofilm performs excellent tolerance to adverse conditions.
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zhengzhaomingb@sina.com.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Du
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Bian
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yanzhuo
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Baihang
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000641:Ammonia; D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D018441:Biofilms; D019149:Bioreactors; D052837:Chemoautotrophic Growth; D058440:Denitrification; D007700:Kinetics; D008962:Models, Theoretical; D058465:Nitrification; D014865:Waste Disposal, Fluid
chemical_list: D064751:Ammonium Compounds; D000641:Ammonia
keywords: Ammonium; Anammox; Free ammonia; Haldane model; Kinetic characteristics; SNAD biofilm