title: Culturing and Cryobanking Human Neural Stem Cells.
abstract: The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.
authors.affiliation: ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.|Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia. jcrook@uow.edu.au.|Department of Surgery, St. Vincent's Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia. jcrook@uow.edu.au.
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Crook
authors.affiliation: ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia.|Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia.
authors.forename: Eva
authors.initials: E
authors.lastname: Tomaskovic-Crook
mesh_terms: D018070:Biological Specimen Banks; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000502:physiology; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology
keywords: Cryopreservation; Culture; Expansion; Freezing; Human neural stem cells; Passaging