title: Immune responses to vaccination and infection with Mycoplasma gallisepticum in turkeys.
abstract: Infection with Mycoplasma gallisepticum induces severe lymphoproliferative lesions in multiple sites along the respiratory tract in chickens and turkeys. These immunopathological responses have been well-characterized in chickens, but have not been studied closely in turkeys. The aim of the study described here was to examine the immune responses of turkeys after live vaccination and infection with M. gallisepticum. In a strain comparison study, the mean log10antibody titre of birds exposed to an aerosol culture of M. gallisepticum strain Ap3AS was found to be significantly higher at day 14 than that of birds exposed to strain 100809/31. In a dose-response study, there was a significant difference in the mean log10antibody titre between birds exposed to mycoplasma broth and birds exposed to the highest dose of strain Ap3AS at day 7 after exposure. Immunohistochemical analysis of the tracheal mucosa and the air sacs revealed similar patterns of distribution of CD4+and CD8+lymphocytes to those seen in the tracheal mucosa of chickens, implicating these cell types in the pathogenesis of respiratory mycoplasmosis in turkeys. Turkeys that had been vaccinated with M. gallisepticum GapA+ts-11 had significantly higher antibody titres than unvaccinated birds at both 7 and 14 days after challenge with strain Ap3AS. Vaccination with GapA+ts-11 protected against the lymphoproliferative response to infection with virulent M. gallisepticum in both the tracheal mucosa and the air sacs, suggesting that this strain may be a useful vaccine candidate for use in turkeys.
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Dinidu S
authors.initials: DS
authors.lastname: Wijesurendra
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Anna
authors.initials: A
authors.lastname: Kanci
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Tivendale
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Devlin
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Nadeeka K
authors.initials: NK
authors.lastname: Wawegama
authors.affiliation: b Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Werribee , Australia.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bacci
authors.affiliation: b Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Werribee , Australia.
authors.forename: Amir H
authors.initials: AH
authors.lastname: Noormohammadi
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Philip F
authors.initials: PF
authors.lastname: Markham
authors.affiliation: a Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences , The University of Melbourne , Parkville , Australia.
authors.forename: Glenn F
authors.initials: GF
authors.lastname: Browning
mesh_terms: D000400:Air Sacs / Q000166:cytology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D017252:CD3 Complex / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D009175:Mycoplasma Infections / Q000517:prevention & control / Q000662:veterinary*; D045686:Mycoplasma gallisepticum / Q000276:immunology*; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000502:physiology; D014132:Trachea / Q000166:cytology; D014422:Turkeys; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D017252:CD3 Complex; D016827:CD8 Antigens; D007074:Immunoglobulin G
keywords: ELISA; Mycoplasma gallisepticum; T lymphocytes; Turkeys; immune responses; pathogenesis; respiratory mycoplasmosis; vaccines