title: Yap/Taz transcriptional activity is essential for vascular regression via Ctgf expression and actin polymerization.
abstract: Vascular regression is essential to remove redundant vessels during the formation of an efficient vascular network that can transport oxygen and nutrient to every corner of the body. However, no mechanism is known to explain how major blood vessels regress during development. Here we use the dorsal part of the caudal vein plexus (dCVP) in Zebrafish to investigate the mechanism of regression and discover a new role of Yap/Taz in vascular regression. During regression, Yap/Taz is activated by blood circulation in the endothelial cells. This leads to induction of Ctgf and actin polymerization. Interference with Yap/Taz activation decreased Ctgf production, which decreased actin polymerization and vascular regression. These results implicate a novel role of Yap/Taz in vascular regression.
authors.affiliation: Laboratory of Function and Morphology, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1 Bunkyo-ku, Tokyo, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Nagasawa-Masuda
authors.affiliation: Laboratory of Function and Morphology, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1 Bunkyo-ku, Tokyo, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Terai
mesh_terms: D000199:Actins / Q000378:metabolism*; D000217:Acyltransferases; D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis*; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D060172:Morpholinos / Q000235:genetics; D018919:Neovascularization, Physiologic; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D000091102:YAP-Signaling Proteins; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D060172:Morpholinos; D015534:Trans-Activators; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C583457:Yes-associated protein (yap), zebrafish; D029961:Zebrafish Proteins; D055513:Connective Tissue Growth Factor; D000217:Acyltransferases; C512741:tafazzin protein, zebrafish