title: Sustainable hydroxypropyl methylcellulose/xyloglucan/gentamicin films with antimicrobial properties.
abstract: Hydroxypropyl methylcellulose (HPMC) and xyloglucan (XG) crosslinked with citric acid over a range of HPMC/XG weight ratios formed sustainable blend films characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, tensile tests, circular dichroism and determination of inhibitory activity against Staphylococcus aureus and Escherichia coli. Both in solution and in the crosslinked films, HPMC chains lost the original ordered conformation upon interacting with XG, giving rise to an entropic gain. The highest values of tensile strength (25MPa) and Young's modulus (689MPa) occurred for the 50:50 HPMC/XG blend films. In vitro loading of gentamicin sulfate (GS) in the films amounted to 0.18±0.05 -0.37±0.05g of GS per g polymer. At pH 7.4 and 37°C, the GS release kinetics from the films fitted with the Korsmeyer-Peppas model revealed a non-Fickian release mechanism with diffusional coefficient n∼0.7. The cross-linked films of HPMC, XG and their blends loaded with GS showed outstanding antibacterial activity against Staphylococcus aureus and Escherichia coli, disclosing their potential for novel biomedical applications.
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, Brazil.
authors.forename: Stalin
authors.initials: S
authors.lastname: Kondaveeti
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, Brazil.
authors.forename: Tatiana C
authors.initials: TC
authors.lastname: Damato
authors.affiliation: Biochemistry Department, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Carmona-Ribeiro
authors.affiliation: BioPol, Universidade Federal do Paraná, Paraná, Brazil.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Sierakowski
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, São Paulo, Brazil. Electronic address: dfsp@usp.br.
authors.forename: Denise Freitas Siqueira
authors.initials: DF
authors.lastname: Petri
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology*; D005936:Glucans / Q000494:pharmacology*; D065347:Hypromellose Derivatives / Q000494:pharmacology*; D008747:Methylcellulose; D013211:Staphylococcus aureus / Q000187:drug effects; D014990:Xylans / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D005839:Gentamicins; D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D065347:Hypromellose Derivatives; D008747:Methylcellulose
keywords: Antibacterial properties; Blend films; Crosslinking; Gentamicin sulfate; Hydroxypropyl methylcellulose (HPMC); Xyloglucan (XG)