title: A Member of the14-3-3Gene Family inBrachypodium distachyon, BdGF14d, Confers Salt Tolerance in Transgenic Tobacco Plants.
abstract: Plant 14-3-3 proteins are involved in diverse biological processes, but for the model monocotyledonous species,Brachypodium distachyon, their roles in abiotic stress tolerance are not well understood. In this study, a total of eightBd14-3-3genes were identified fromB. distachyonand these were designated respectively asBdGF14a-BdGF14g. The qRT-PCR analyses of 3-month-old plants ofB. distachyonshowed that these genes were all expressed in the stems, leaves, and spikelets. By contrast, most of the plants had relatively lower transcriptional levels in their roots, except for theBdGF14ggene. The different expression profiles of theBd14-3-3sunder various stress treatments, and the diverse interaction patterns between Bd14-3-3s and BdAREB/ABFs, suggested that these gene products probably had a range of functions in the stress responses. The NaCl-inducedBd14-3-3gene,BdGF14d, was selected for overexpression in tobacco. BdGF14d was found to be localized throughout the cell and it conferred enhanced tolerance to salt in the transgenic plants. Lowered contents of malondialdehyde, H2O2, and Na+, and lower relative electronic conductance (Rec%), yet greater activities of catalase and peroxidase, were observed in the overexpressing plants. Higher photosynthetic rate, transpiration rate, stomatal conductance, and water use efficiency were measured in the transgenic lines. Following abscisic acid (ABA) or NaCl treatment, stomatal aperture in leaves of theBdGF14d-overexpression plants was significantly lower than in leaves of the wild type (WT) controls. The stress-related marker genes involved in the ABA signaling pathway, the reactive oxygen species (ROS)-scavenging system, and the ion transporters were all up-regulated in theBdGF14d-overexpressing plants as compared with WT. Taken together, these results demonstrate that theBd14-3-3genes play important roles in abiotic stress tolerance. The ABA signaling pathway, the ROS-scavenging system, and ion transporters were all involved in enhancing the tolerance to salt stress in theBdGF14d-overexpression plants.
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Wu
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Qingchen
authors.initials: Q
authors.lastname: Luo
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Wei
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Kexiu
authors.initials: K
authors.lastname: Li
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Chang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Guangxiao
authors.initials: G
authors.lastname: Yang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: He
keywords: ABA signaling; B. distachyon; Bd14-3-3s; BdGF14d; ROS-scavenging system; salt tolerance