title: Sensitive detection of PDT-induced cell damages with luminescent oxygen nanosensors.
abstract: In this work luminescent nanosensors specifically created for intracellular oxygen (ic-O2) were utilized to assess photodynamic therapy (PDT) -induced cell damages. Firstly, ic-O2was demonstrated to be consumed much faster than extracellular O2with respective O2nanosensors. Using the ic-O2nanosensors, PDT-treated cells with different degree of impairment were then resolved according to the oxygen consumption rate (OCR). The evolving trend of cytotoxicity derived from OCRs was in agreement with cell viability obtained from 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Moreover, the direct damage of PDT on cell mitochondria was successfully detected by monitoring respiration instantly after PDT treatment, which is actually beyond the scope of MTT assay. These results suggest that fluorescence sensing of ic-O2-associated cell respiration is promising and even may become a standardized method, complementary to MTT assay, to evaluate PDT-induced cytotoxicity.
authors.affiliation: College of Science, Minzu University of China, Beijing 100081, People's Republic of China. Key Laboratory of Luminescence and Optical Information, Ministry of Education, Institute of Optoelectronic Technology, Beijing Jiaotong University, Beijing 100044, People's Republic of China.
authors.forename: Hong-Ru
authors.initials: HR
authors.lastname: Ma
authors.forename: Hong-Shang
authors.initials: HS
authors.lastname: Peng
authors.forename: Fang-Tian
authors.initials: FT
authors.lastname: You
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Ping
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Guo
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D019069:Cell Respiration; D002470:Cell Survival; D006801:Humans; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption; D010778:Photochemotherapy; D017319:Photosensitizing Agents; D026082:Singlet Oxygen
chemical_list: D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D010100:Oxygen