title: Preparation and bioavailability of calcium-chelating peptide complex from tilapia skin hydrolysates.
abstract: BACKGROUND
  With the continuous improvement in material life, the generation of fish by-products and the market demand for calcium supplements have been increasing in China. Therefore a calcium-chelating peptide complex (CPC) from tilapia skins was prepared and its effect on calcium (Ca)-deficient mice was investigated.
  
  
  RESULTS
  The molecular weight distribution of CPC mainly ranged from 2000 to 180 Da, and its contents of complete amino acids and free amino acids were 85.30 and 8.67% (w/w) respectively. Scanning electron microscopy images and Fourier transform infrared data revealed that Ca crystals were bound with gelatin hydrolysates via interaction between Ca ions and NH/CN groups. When Ca-deficient mice were fed CPC and CaCO3respectively for 4 weeks, no significant differences in serum biochemistry or bone mineral density were found. However, the length, weight, Ca content and hydroxyproline content of the femur, Ca absorption and body weight gain of mice fed CPC were significantly higher than those of mice fed CaCO3.
  
  
  CONCLUSIONS
  It is concluded that the prepared CPC could promote bone formation via better bioavailability of Ca and an increase in bone collagen. © 2017 Society of Chemical Industry.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, China.|Xiamen Key Laboratory of Marine Functional Food, Xiamen, China.|Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, China.|Xiamen Key Laboratory of Marine Functional Food, Xiamen, China.|Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, China.
authors.forename: Xujian
authors.initials: X
authors.lastname: Qiu
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, China.|Xiamen Key Laboratory of Marine Functional Food, Xiamen, China.|Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, China.
authors.forename: Gengxin
authors.initials: G
authors.lastname: Hao
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen, China.|Xiamen Key Laboratory of Marine Functional Food, Xiamen, China.|Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, China.
authors.forename: Wuyin
authors.identifier: http://orcid.org/0000-0003-2996-5423
authors.initials: W
authors.lastname: Weng
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D002136:Calcium, Dietary / Q000032:analysis / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D005269:Femur / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D012867:Skin / Q000737:chemistry*; D017210:Tilapia
chemical_list: D000596:Amino Acids; D002136:Calcium, Dietary; D029941:Fish Proteins; D010455:Peptides; D005780:Gelatin; D003094:Collagen; D002118:Calcium
keywords: bone mineral; calcium absorption; calcium-chelating peptide; tilapia skin; ultrafiltration