title: Root extractive fromDaphne genkwabenefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts.
abstract: Wound healing is the main problem in the therapy of anal fistula (AF).Daphne genkwaroot has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms ofD. genkwaroot treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without theD. genkwaextractive. Wound healing times were compared and granulation tissues were collected.In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment ofTNF-α(10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects ofD. genkwaroot extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels ofTGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) andMEK/ERKsignalling pathways were investigated bothin vivoandin vitroResults showed thatD. genkwaroot extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions ofCOL1A1, COL3A1, Timp-1, c-fosandCyclin D1, while reduced the expression ofMMP-3Further detection presented thatMEK/ERKsignalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive fromD. genkwaroot could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions ofCOL1A1andCOL3A1.
authors.affiliation: First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, P.R. China.|Department of Anorectal Surgery, The First People's Hospital of Lianyungang, Lianyungang 222002, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Anorectal Surgery, Affiliated Hospital of Nanjing Uiversity of Chinese Medicine, Nanjing 210029, P.R. China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Xu
authors.affiliation: Department of Anorectal Surgery, Affiliated Hospital of Nanjing Uiversity of Chinese Medicine, Nanjing 210029, P.R. China renjie_shi_nj@163.com.|Department of Anorectal Surgery, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, P.R. China.
authors.forename: Ren-Jie
authors.initials: RJ
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000032:analysis / Q000235:genetics*; D032364:Daphne / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D012003:Rectal Fistula / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D003094:Collagen
keywords: Anal fistula; Cell signaling pathways; Daphne genkwa; Wounds healing; human skin fibroblasts