title: Serum levels of 9α,11β-PGF2and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis.
abstract: Anaphylaxis is a life-threatening hypersensitivity reaction. To identify biomarkers for the condition, we assessed serum levels of apolipoprotein (Apo)A and ApoE. We found a reduction of both lipoproteins in anaphylactic mice as well as in orally challenged food allergic patients. We then compared patients after acute anaphylaxis with several control groups (nonallergic, history of allergen-triggered anaphylaxis, acute cardiovascular/febrile reactions). In this unpaired setting, ApoE levels were unaltered, while ApoA1 was reduced in the anaphylactic group. Although unable to discriminate between anaphylaxis and cardiovascular/febrile reactions, ROC curve analysis revealed a reasonably high area under the curve (AUC) of 0.91 for ApoA1. Serum 9α,11ß-PGF2, recently identified as a suitable biomarker for anaphylaxis, outperformed ApoA1 with AUC=0.95. Intriguingly however its power further increased upon combination of both mediators reaching AUC=1.Our data suggest that ApoA1 combined with 9α,11ß-PGF2represents a useful composite biomarker of anaphylaxis, achieving superior diagnostic power over either factor alone.
authors.affiliation: Department of Dermatology and Allergy, Allergy Center Charité, CCM, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wittenberg
authors.affiliation: Department of Dermatology and Allergy, Allergy Center Charité, CCM, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Nassiri
authors.affiliation: Department of Dermatology and Allergy, Allergy Center Charité, CCM, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Francuzik
authors.affiliation: Proteome Factory AG, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Lehmann
authors.affiliation: Department of Dermatology and Allergy, Allergy Center Charité, CCM, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Babina
authors.affiliation: Department of Dermatology and Allergy, Allergy Center Charité, CCM, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: M
authors.identifier: http://orcid.org/0000-0002-3449-1245
authors.initials: M
authors.lastname: Worm
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000175:diagnosis*; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood*; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D015237:Dinoprost / Q000097:blood*; D006801:Humans; D051379:Mice; D011237:Predictive Value of Tests; D012372:ROC Curve
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D015415:Biomarkers; D015237:Dinoprost
keywords: anaphylaxis; apolipoprotein A1; apolipoprotein E; biomarkers; mast cell activation