title: Vitellogenin receptor selectively endocytoses female-specific and highly-expressed hemolymph proteins in the silkworm, Bombyx mori.
abstract: VgR, a member of the LDLR family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, BmVgR has been shown to interact with BmSP1 in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with BmVg and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells. Parallel immunofluorescence showed that most BmVg and BmVgR were localized in the inner oocyte membrane, showing tissue localization similar to that of BmVg labeled with pHrodo Red absorbed by the ovaries on day 2 of pupation. Although BmSP1 showed localization similar to BmVgR during the same phase, little BmSP1 was present in the ovary. Additionally, BmSP1 did not exist in ovaries when the ovaries contained BmVgR on day 5 of pupation, suggesting that BmSP1 in the ovaries was not endocytosed by BmVgR. In summary, BmVgR could facilitate uptake of BmVg by developing oocytes, but did not modulate in the transport of BmSP1.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Chaoshan
authors.initials: C
authors.lastname: Han
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Enxiang
authors.initials: E
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Guanwang
authors.initials: G
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Yinying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Yandi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D012831:Bombyx / Q000166:cytology* / Q000378:metabolism*; D004527:Egg Proteins / Q000378:metabolism*; D004705:Endocytosis; D005260:Female; D006458:Hemolymph / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D019476:Insect Proteins; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor
keywords: endocytose; endocytosis; protéine de stockage 1; récepteur de la vitellogénine; silkworm; storage protein 1; ver à soie; vitellogenin; vitellogenin receptor