title: Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.
abstract: Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, UK.
authors.forename: Florian
authors.initials: F
authors.lastname: Ströhl
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: Julie Qiaojin
authors.identifier: http://orcid.org/0000-0002-2669-6478
authors.initials: JQ
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, UK.
authors.forename: Romain F
authors.initials: RF
authors.lastname: Laine
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: Hovy Ho-Wai
authors.initials: HH
authors.lastname: Wong
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: Vasja
authors.initials: V
authors.lastname: Urbančič
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.
authors.forename: Roberta
authors.initials: R
authors.lastname: Cagnetta
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK. ceh33@cam.ac.uk.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Holt
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, UK. cfk23@cam.ac.uk.
authors.forename: Clemens F
authors.initials: CF
authors.lastname: Kaminski
mesh_terms: D000199:Actins / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D057054:Molecular Imaging / Q000379:methods; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000378:metabolism*; D014981:Xenopus
chemical_list: D000199:Actins; D012333:RNA, Messenger