title: A novel multicopper oxidase (laccase) from cyanobacteria: Purification, characterization with potential in the decolorization of anthraquinonic dye.
abstract: A novel extracellular laccase enzyme produced from Spirulina platensis CFTRI was purified by ultrafiltration, cold acetone precipitation, anion exchange and size exclusion chromatography with 51.5% recovery and 5.8 purification fold. The purified laccase was a monomeric protein with molecular mass of ~66 kDa that was confirmed by zymogram analysis and peptide mass fingerprinting. The optimum pH and temperature of the enzyme activity was found at 3.0 and 30°C using ABTS as substrate but the enzyme was quite stable at high temperature and alkaline pH. The laccase activity was enhanced by Cu+2, Zn+2 and Mn+2. In addition, the dye decolorization potential of purified laccase was much higher in terms of extent as well as time. The purified laccase decolorized (96%) of anthraquinonic dye Reactive blue- 4 within 4 h and its biodegradation studies was monitored by UV visible spectra, FTIR and HPLC which concluded that cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment.
authors.affiliation: Cyanobacterial Biotechnology laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Sumbul
authors.initials: S
authors.lastname: Afreen
authors.affiliation: Department of Biotechnology, Jamia Millia Islamia, New Delhi, India.
authors.forename: Tooba Naz
authors.initials: TN
authors.lastname: Shamsi
authors.affiliation: Department of Biotechnology, Jamia Millia Islamia, New Delhi, India.
authors.forename: Mohd Affan
authors.initials: MA
authors.lastname: Baig
authors.affiliation: Cyanobacterial Biotechnology laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Nadeem
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Department of Biotechnology, Jamia Millia Islamia, New Delhi, India.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Fatima
authors.affiliation: Department of Biotechnology, Jamia Millia Islamia, New Delhi, India.
authors.forename: M Irfan
authors.initials: MI
authors.lastname: Qureshi
authors.affiliation: Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Md Imtaiyaz
authors.initials: MI
authors.lastname: Hassan
authors.affiliation: Cyanobacterial Biotechnology laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Tasneem
authors.identifier: http://orcid.org/0000-0003-3712-8692
authors.initials: T
authors.lastname: Fatma
mesh_terms: D002384:Catalysis; D002850:Chromatography, Gel; D004396:Coloring Agents / Q000737:chemistry; D000458:Cyanobacteria / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000302:isolation & purification* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004396:Coloring Agents; D042845:Laccase