title: Differential Expression of Toll-Like Receptor Signaling Pathway Is Associated with Microscopic Polyangiitis in Peripheral Blood Neutrophils.
abstract: Constitutive or excessive activation of Toll-like receptor (TLR) signaling pathway can disrupt the body's immune tolerance to autoantigen, thus promoting the development of autoimmune disease. However, the expression profile of TLR signaling pathway in peripheral blood neutrophils in the pathogenesis of microscopic polyangiitis (MPA) remains unclear. Thus, improved understanding of the pathobiology of this disease may aid in the development of therapeutic targets for patients with MPA. In the present study, we assessed the expression of TLR signaling pathway-related genes in peripheral blood neutrophils in patients with MPA. PCR array analysis was performed on 20 patients with MPA and 12 healthy controls. Gene expression proﬁle was performed using the human TLR for autoimmunity and inflammation PCR array of Genecopoeia, containing 84 genes related to TLR signaling pathway and six house-keeping genes. We then used quantitative real-time PCR to validate the array test. The array results identified 13 upregulated genes and 5 genes which were downregulated. The resulting qRT-PCR was consistent with the findings by PCR array. Our results suggest that peripheral blood neutrophils display changes in the expression of TLR signaling pathway-related genes associated with the pathogenesis of microscopic polyangiitis.
authors.affiliation: a Department of Nephrology , The Second Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Lai
authors.affiliation: a Department of Nephrology , The Second Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xue
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Liao
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Wei
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Linlin
authors.initials: L
authors.lastname: He
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Aimei
authors.initials: A
authors.lastname: Gong
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of Guangxi Medical University , Nanning , China.
authors.forename: Minwen
authors.initials: M
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D001775:Blood Circulation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D055953:Microscopic Polyangiitis / Q000276:immunology*; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D015398:Signal Transduction; D051201:Toll-Like Receptor 10 / Q000235:genetics / Q000378:metabolism; D051216:Toll-Like Receptor 6 / Q000235:genetics / Q000378:metabolism; D051199:Toll-Like Receptor 7 / Q000235:genetics / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome; D055815:Young Adult
chemical_list: C475955:TLR10 protein, human; C495368:TLR6 protein, human; C495348:TLR7 protein, human; D051201:Toll-Like Receptor 10; D051216:Toll-Like Receptor 6; D051199:Toll-Like Receptor 7; D051193:Toll-Like Receptors
keywords: Array; TLR; gene expression; microscopic polyangiitis