title: Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates.
abstract: The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Rebeca M
authors.initials: RM
authors.lastname: Torrente-Rodríguez
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Ruiz-Valdepeñas Montiel
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Pedrero
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Meryem
authors.initials: M
authors.lastname: Farchado
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Vargas
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Manuel de Villena
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Garranzo-Asensio
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: José M
authors.identifier: http://orcid.org/0000-0003-2271-1383
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D045744:Cell Line, Tumor; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D051519:Fibroblast Growth Factor 4 / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D061986:MCF-7 Cells; D008280:Magnetics
chemical_list: D014408:Biomarkers, Tumor; C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4