title: Evaluation of the toxic effect of endocrine disruptor Bisphenol A (BPA) in the acute and chronic toxicity tests with Pomacea lineata gastropod.
abstract: Bisphenol A (BPA) is a plasticizer and a risk when it interacts with organisms, and can cause changes in the development and reproduction of them. This study aimed to evaluate the effects of BPA, by acute and chronic toxicity tests with neonates and adults of Pomacea lineata. Adults and neonates were divided into groups exposed to BPA (1-20mg/L), or 17β-estradiol (1mg/L) and control in the acute and chronic toxicity tests. Behavior, heart rate, reproduction and hemolymph biochemical analysis were measured. In the acute toxicity test, the 96-h LC50with adults was 11.09 and with neonates was 3.14mg/L. In this test, it was observed lethargic behavior and an increase of 77.6% of aspartate aminotransferase in the adults' hemolymph (p<0.05); and neonates' heart rate decreased 72.7% (p<0.05). In the chronic toxicity test, it was observed behaviors associated with reproduction, as Copulate, in the groups exposed to BPA. The results that were found in this study proved that BPA is a potentially toxic agent to Pomacea lineata according to biological parameters evaluated. These data contribute to the understanding of BPA toxic effects' in the aquatic invertebrates.
authors.affiliation: Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil.
authors.forename: André Lucas Correa
authors.initials: ALC
authors.lastname: de Andrade
authors.affiliation: Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil.
authors.forename: Priscila Rafaela Leão
authors.initials: PRL
authors.lastname: Soares
authors.affiliation: Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil.
authors.forename: Stephannie Caroline Barros Lucas
authors.initials: SCBL
authors.lastname: da Silva
authors.affiliation: Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil.
authors.forename: Marília Cordeiro Galvão
authors.initials: MCG
authors.lastname: da Silva
authors.affiliation: Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil.
authors.forename: Thamiris Pinheiro
authors.initials: TP
authors.lastname: Santos
authors.affiliation: Unidade Acadêmica de Serra Talhada (UAST), Universidade Federal Rural de Pernambuco, Avenida Gregório Ferraz Nogueira s/n, 56909-535, José Ramos, Serra Talhada, PE, Brazil.
authors.forename: Marilia Ribeiro Sales
authors.initials: MRS
authors.lastname: Cadena
authors.affiliation: Departamento de Medicina Veterinária (DMV), Universidade Federal de Pernambuco (UFPE), Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil.
authors.forename: Pierre Castro
authors.initials: PC
authors.lastname: Soares
authors.affiliation: Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900, Dois Irmãos, Recife, PE, Brazil. Electronic address: pabyton.cadena@ufrpe.br.
authors.forename: Pabyton Gonçalves
authors.initials: PG
authors.lastname: Cadena
mesh_terms: D000367:Age Factors; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001522:Behavior, Animal / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D049851:Gastropoda / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D006458:Hemolymph / Q000187:drug effects; D008297:Male; D010636:Phenols / Q000633:toxicity*; D023382:Toxicity Tests, Acute / Q000379:methods*; D036401:Toxicity Tests, Chronic / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001219:Aspartate Aminotransferases; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Xenoestrogen; animal behavior; biochemical profile; ecotoxicology; mollusk; reproduction