title: miR-1193 Suppresses the Proliferation and Invasion of Human T-Cell Leukemia Cells Through Directly Targeting the Transmembrane 9 Superfamily 3 (TM9SF3).
abstract: miRNAs have been involved in various types of cancer, including T-cell leukemia. In this study, the role of miR-1193 in the proliferation and invasion of T-cell leukemia cells was explored. First, we found that miR-1193 was sharply downregulated in T-cell leukemia cells when compared with normal T cells. miR-1193 markedly decreased the proliferation and invasion in Jurkat human T-cell leukemia cells. Transmembrane 9 superfamily 3 (TM9SF3) was then predicted to be a potential target gene of miR-1193, the levels of which displayed a strongly negative correlation with miR-1193 levels in T-cell leukemia patients. We confirmed that TM9SF3 was a target gene of miR-1193 by luciferase reporter gene assay. Finally, gene overexpression and knockdown experiments in Jurkat cells revealed that TM9SF3 positively regulated cell proliferation and invasion.
authors.forename: Liyun
authors.initials: L
authors.lastname: Shen
authors.forename: Xingjun
authors.initials: X
authors.lastname: Du
authors.forename: Hongyan
authors.initials: H
authors.lastname: Ma
authors.forename: Shunxi
authors.initials: S
authors.lastname: Mei
mesh_terms: D000328:Adult; D002352:Carrier Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D015536:Down-Regulation; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; C437715:TM9SF3 protein, human