title: Electrophysiological dysfunction induced by anti-ribosomal P protein antibodies injection into the lateral ventricle of the rat brain.
abstract: Objective Anti-ribosomal P antibodies (anti-P) are strongly associated with neuropsychiatric lupus. This study was designed to determine whether these antibodies are capable of causing electro-oscillogram (EOSG) and behavior alterations in rats. Methods IgG fraction anti-P positive and affinity-purified anti-P antibodies were injected intraventricularly in rats. Sequential cortical and subcortical EOSGs were analyzed during 30 days. IgG anti-Ro/SS-A and normal IgG were used as controls. Results All 13 animals injected with IgG anti-P demonstrated a high prevalence of polyspikes, diffusely distributed in hippocampal fields and cerebral cortex. These abnormalities persisted approximately a month. Remarkably, an identical electrical disturbance was observed with the inoculation of affinity-purified anti-P antibodies. The EOSG alterations were associated with behavioral disorders with varying degrees of severity in every animal injected with anti-P. In contrast, no changes in EOSG or behavioral disturbances were observed in the control group. Conclusion Our study indicates that anti-P antibodies can directly induce electrophysiological dysfunction in central nervous system particularly in hippocampus and cortex associated with behavior disturbances.
authors.affiliation: 1 Rheumatology Division from Faculdade de Medicina da Universidade de São Paulo e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Gaburo
authors.affiliation: 2 Federal University of Bahia, Salvador-BA, Brazil.
authors.forename: J Freire
authors.initials: JF
authors.lastname: de Carvalho
authors.affiliation: 3 (in memoriam) Laboratory of Functional Neurosurgery, Department of Neurology and Neurosurgery from Faculdade de Medicina da Universidade de São Paulo e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Timo-Iaria
authors.affiliation: 1 Rheumatology Division from Faculdade de Medicina da Universidade de São Paulo e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Bueno
authors.affiliation: 4 Formerly of Oklahoma University Health Sciences Center, Department of Medicine, Oklahoma City, OK, USA.
authors.forename: M
authors.initials: M
authors.lastname: Reichlin
authors.affiliation: 1 Rheumatology Division from Faculdade de Medicina da Universidade de São Paulo e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: V S T
authors.initials: VS
authors.lastname: Viana
authors.affiliation: 1 Rheumatology Division from Faculdade de Medicina da Universidade de São Paulo e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Bonfá
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000008:administration & dosage / Q000009:adverse effects; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000009:adverse effects; D007267:Injections; D020547:Lateral Ventricles / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000503:physiopathology; D008297:Male; D001523:Mental Disorders / Q000139:chemically induced* / Q000503:physiopathology; D051381:Rats; D012269:Ribosomal Proteins / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D012269:Ribosomal Proteins
keywords: Systemic lupus erythematosus; anti-P ribosomal antibody; autoimmunity; central nervous system; experimental model; psychosis