title: Clinical perspectives of dried blood spot protein quantification using mass spectrometry methods.
abstract: Although dried blood spot (DBS) sampling methods have been used since the 1960s, they have recently attracted renewed interest because of the development of new clinical applications. In addition to their other advantages, DBS methods can now be used to quantify many blood proteins using the latest highly sensitive and robust, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) approaches such as multiple reaction monitoring. The DBS blood sampling approach could provide a useful alternative means of conducting blood sampling for routine clinical purposes and patients' follow-up. In this review, we examine the current use of DBS for LC-MS/MS protein quantification in clinical settings and discuss potential clinical applications.
authors.affiliation: a Laboratory of Biochemistry and Clinical Proteomics, CHU Montpellier , Institute of Regenerative Medicine & Biotherapy , Montpellier , France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
authors.affiliation: a Laboratory of Biochemistry and Clinical Proteomics, CHU Montpellier , Institute of Regenerative Medicine & Biotherapy , Montpellier , France.
authors.forename: Alexia
authors.initials: A
authors.lastname: Picas
authors.affiliation: a Laboratory of Biochemistry and Clinical Proteomics, CHU Montpellier , Institute of Regenerative Medicine & Biotherapy , Montpellier , France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Tiers
authors.affiliation: a Laboratory of Biochemistry and Clinical Proteomics, CHU Montpellier , Institute of Regenerative Medicine & Biotherapy , Montpellier , France.
authors.forename: Jerome
authors.initials: J
authors.lastname: Vialaret
authors.affiliation: a Laboratory of Biochemistry and Clinical Proteomics, CHU Montpellier , Institute of Regenerative Medicine & Biotherapy , Montpellier , France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
mesh_terms: D059788:Dried Blood Spot Testing; D006801:Humans; D013058:Mass Spectrometry
keywords: Dried blood spots; mass spectrometry; multiple reaction monitoring; pre-analytical