title: Recombinant expression and purification of the RNA-binding LARP6 proteins from fish genetic model organisms.
abstract: The RNA-binding proteins that comprise the La-related protein (LARP) superfamily have been implicated in a wide range of cellular functions, from tRNA maturation to regulation of protein synthesis. To more expansively characterize the biological function of the LARP6 subfamily, we have recombinantly expressed the full-length LARP6 proteins from two teleost fish, platyfish (Xiphophorus maculatus) and zebrafish (Danio rerio). The yields of the recombinant proteins were enhanced to >2 mg/L using a tandem approach of an N-terminal His6-SUMO tag and an iterative solubility screening assay to identify structurally stabilizing buffer components. The domain topologies of the purified fish proteins were probed with limited proteolysis. The fish proteins contain an internal, protease-resistant 40 kDa domain, which is considerably more stable than the comparable domain from the human LARP6 protein. The fish proteins are therefore a lucrative model system in which to study both the evolutionary divergence of this family of La-related proteins and the structure and conformational dynamics of the domains that comprise the LARP6 protein.
authors.affiliation: Department of Chemistry and Biochemistry, Texas State University, 601 University Dr., San Marcos, TX, 78666, United States.
authors.forename: José M
authors.initials: JM
authors.lastname: Castro
authors.affiliation: Department of Chemistry and Biochemistry, Texas State University, 601 University Dr., San Marcos, TX, 78666, United States.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Horn
authors.affiliation: Department of Chemistry and Biochemistry, Texas State University, 601 University Dr., San Marcos, TX, 78666, United States.
authors.forename: Xinzhu
authors.initials: X
authors.lastname: Pu
authors.affiliation: Department of Chemistry and Biochemistry, Texas State University, 601 University Dr., San Marcos, TX, 78666, United States. Electronic address: karen.lewis@txstate.edu.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Lewis
mesh_terms: D000818:Animals; D003532:Cyprinodontiformes / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D000072417:Protein Domains; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D029961:Zebrafish Proteins
keywords: Bacterial expression; La-related protein; Protein solubility; RNA binding protein