title: Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase.
abstract: In order to improve the silage fermentation of stylo (Stylosanthes guianensis) in tropical areas, stylo silages were prepared with commercial additives Lactobacillus plantarum Chikuso-1 (CH1), L. rhamnasus Snow Lact L (SN), Acremonium cellulase (CE) and their combination as SN+CE or CH1 + CE, and the fermentation quality, chemical composition and ruminal degradation of these silages were studied. Stylo silages treated with lactic acid bacteria (LAB) or cellulase, the pH value and NH3-N ⁄ total-N were significantly (P < 0.05) decreased while the ruminal degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (aNDFom) and acid detergent fiber (ADFom) were significantly (P < 0.05) increased compared to control. Compared to LAB or cellulase-treated silages, the DM, CP contents and relative feed value (RFV), and the ruminal degradability in LAB plus cellulase-treated silages were significantly (P < 0.05) higher, but the aNDFom content was significantly (P < 0.05) lower. CH1 + CE treatment was more effective in silage fermentation and ruminal degradation than SN+CE treatment. The results confirmed that LAB or LAB plus cellulase treatment could improve the fermentation quality, chemical composition and ruminal degradation of stylo silage. Moreover, the combined treatment with LAB and cellulase may have beneficial synergistic effects on ruminal degradation.
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Li
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, China.
authors.forename: Hanlin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan, China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Zi
authors.affiliation: National Agriculture and Food Research Organization, Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Yimin
authors.identifier: http://orcid.org/0000-0003-2650-5210
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000164:Acremonium / Q000201:enzymology; D000641:Ammonia / Q000032:analysis; D000818:Animals; D002480:Cellulase; D004044:Dietary Proteins / Q000032:analysis; D004063:Digestion; D005285:Fermentation; D006041:Goats / Q000378:metabolism* / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid; D048191:Lactiplantibacillus plantarum; D052201:Lacticaseibacillus rhamnosus; D009584:Nitrogen / Q000032:analysis; D012417:Rumen / Q000378:metabolism* / Q000502:physiology*; D012820:Silage / Q000032:analysis
chemical_list: D004044:Dietary Proteins; D019344:Lactic Acid; D000641:Ammonia; D002480:Cellulase; D009584:Nitrogen
keywords: cellulase; fermentation quality; lactic acid bacteria; ruminal degradation; stylo silage