title: Preparation, Characterization and Properties of Alginate/Poly(γ-glutamic acid) Composite Microparticles.
abstract: Alginate (Alg) is a renewable polymer with excellent hemostatic properties and biocapability and is widely used for hemostatic wound dressing. However, the swelling properties of alginate-based wound dressings need to be promoted to meet the requirements of wider application. Poly(γ-glutamic acid) (PGA) is a natural polymer with high hydrophility. In the current study, novel Alg/PGA composite microparticles with double network structure were prepared by the emulsification/internal gelation method. It was found from the structure characterization that a double network structure was formed in the composite microparticles due to the ion chelation interaction between Ca2+and the carboxylate groups of Alg and PGA and the electrostatic interaction between the secondary amine group of PGA and the carboxylate groups of Alg and PGA. The swelling behavior of the composite microparticles was significantly improved due to the high hydrophility of PGA. Influences of the preparing conditions on the swelling behavior of the composites were investigated. The porous microparticles could be formed while compositing of PGA. Thermal stability was studied by thermogravimetric analysis method. Moreover, in vitro cytocompatibility test of microparticles exhibited good biocompatibility with L929 cells. All results indicated that such Alg/PGA composite microparticles are a promising candidate in the field of wound dressing for hemostasis or rapid removal of exudates.
authors.affiliation: School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. zrtong@163.com.
authors.forename: Zongrui
authors.initials: Z
authors.lastname: Tong
authors.affiliation: School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. cylsy@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. lyang425@yeah.net.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China. tongli29@bnu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.affiliation: Taizhou Roosin Medical Co., Ltd., Taizhou 225300, China. jameschu@roosin.com.
authors.forename: Jiamian
authors.initials: J
authors.lastname: Chu
authors.affiliation: School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. xiaokecen@163.com.
authors.forename: Kecen
authors.initials: K
authors.lastname: Xiao
authors.affiliation: School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. zhouzhiyuandy@163.com.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. dwb194413@126.com.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Dong
authors.affiliation: Taizhou Roosin Medical Co., Ltd., Taizhou 225300, China. rongxingchu@roosin.net.
authors.forename: Xingwu
authors.initials: X
authors.lastname: Chu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002460:Cell Line; D020723:Glucuronic Acid / Q000737:chemistry; D006487:Hemostasis / Q000187:drug effects; D006603:Hexuronic Acids / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011108:Polymers; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D020723:Glucuronic Acid; D002118:Calcium
keywords: alginate; emulsification/internal gelation method; microparticle; poly(γ-glutamic acid); swelling behavior