title: Pattern of LRP6 gene expression in tumoral tissues of colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is known as one of the most leading causes of death from cancer in the world. Wnt signaling pathway is the most important signaling pathway activated in this type of cancer. The low-density lipoprotein (LDL) receptor-related protein-6 (LRP6) functions as a co-receptor in order to trigger this signaling pathway.
  
  
  OBJECTIVE
  The expression of LRP6 was studied in cell lines, tumoral and normal adjacent tissues from patients affected with colorectal cancer.
  
  
  METHODS
  The expression of LRP6 was assayed by quantitative Real time PCR.
  
  
  RESULTS
  LRP6 is overexpressed at the transcript and protein level in HCT116 and SW480 in comparison to HUVEC as the non-cancerous cell. Furthermore, LRP6 was significantly up-regulated in 61% of human malignant tissues (p-value = 0.0008). In inherited type of disease, this upregulation was lower than other types of colorectal cancer, while in rectal cancer the overexpression was significantly higher (p-value = 0.039). Gene expression was not correlated with the age, gender and the stage of disease.
  
  
  CONCLUSIONS
  Due to the profound overexpression of LRP6 in sporadic and rectal types of cancer compared to normal colonic ones, antagonist related approaches can be promising for targeted therapies of cancer.
authors.affiliation: Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Rismani
authors.affiliation: Department of Surgery, Division of Colo-Rectal Surgery, Imam Khomeini Medical Complex, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Sadegh
authors.initials: MS
authors.lastname: Fazeli
authors.affiliation: Cancer Institute of Iran, Imam Khomeini Medical Complex, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Habibollah
authors.initials: H
authors.lastname: Mahmoodzadeh
authors.affiliation: Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Aghdas
authors.initials: A
authors.lastname: Movassagh
authors.affiliation: Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Sina
authors.initials: S
authors.lastname: Azami
authors.affiliation: Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Karimipoor
authors.affiliation: Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Teimoori-Toolabi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D060488:Low Density Lipoprotein Receptor-Related Protein-6 / Q000235:genetics* / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C555475:LRP6 protein, human; D060488:Low Density Lipoprotein Receptor-Related Protein-6
keywords: Colorectal neoplasm; LRP6 protein; Wnt signaling pathway; gene expression; human