title: Fractionation, amino acid profiles, antimicrobial and free radical scavenging activities of Citrullus lanatus seed protein.
abstract: In the present study, a modified Osborne fractionation method was followed to isolate albumin (Calb), globulin (Cglo), prolamin (Cpro) and glutelin (Cglu) successively from seeds of Citrullus lanatus (watermelon). This research work was undertaken to investigate the antimicrobial and antioxidant activities of isolated protein fractions of C. lanatus seed. Amino acid composition and molecular weight distribution were determined to establish their relationship with antimicrobial and antioxidant activity. Among all the fractions, Cprowas found to be most effective against A. baumannii followed by Calband Cglo. The results showed that growth of inhibition of these protein fractions differ significantly from each other (p ≤ 0.05). In view of antioxidant potential, Cgloexhibited strongest antioxidant capacity while Cglushowed weakest antioxidant potential.
authors.affiliation: a Department of Pharmacognosy, School of Pharmaceutical Sciences , Siksha 'O' Anusandhan University , Bhubaneswar , India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Dash
authors.affiliation: a Department of Pharmacognosy, School of Pharmaceutical Sciences , Siksha 'O' Anusandhan University , Bhubaneswar , India.
authors.forename: Goutam
authors.initials: G
authors.lastname: Ghosh
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000032:analysis* / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D005591:Chemical Fractionation; D029773:Citrullus / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005983:Glutens / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D005983:Glutens
keywords: Citrullus lanatus; antimicrobial; antioxidant; globulin; prolamin