title: The heterologous expression of a chrysanthemum TCP-P transcription factor CmTCP14 suppresses organ size and delays senescence in Arabidopsis thaliana.
abstract: TCP transcription factors are important for plant growth and development, but their activity in chrysanthemum (Chrysanthemum morifolium) has not been thoroughly explored. Here, a chrysanthemum TCP-P sequence, which encodes a protein harboring the conserved basic helix-loop-helix (bHLH) motif, was shown to be related phylogenetically to the Arabidopsis thaliana gene AtTCP14. A yeast-one hybrid assay showed that the encoding protein had no transcriptional activation ability, and a localization experiment indicated that it was localized in the nucleus. Transcription profiling established that the gene was most active in the stem and leaf. Its heterologous expression in A. thaliana down-regulated certain cell cycle-related genes, reduced the size of various organs and increased the chlorophyll and carotenoid contents of the leaf which led to delayed senescence and a prolonged flowering period. Moreover, by screening the cDNA library of chrysanthemum, we found that the CmTCP14 can interact with CmFTL2 and some CmDELLAs.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2014204028@njau.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2014104097@njau.edu.cn.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: hb@njau.edu.cn.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: sherlock47@126.com.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: aiping_song@aliyun.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2015104094@njau.edu.cn.
authors.forename: Yueheng
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: jiangjiafu@njau.edu.cn.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chenfd@njau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D031202:Chrysanthemum / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D009929:Organ Size / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics / Q000502:physiology
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Chrysanthemum morifolium; Leaf senescence; Organ size; TCP transcription factor