title: Synthesis of a chitosan-based photo-sensitive hydrogel and its biocompatibility and biodegradability.
abstract: Hydroxyethyl chitosan (HECTS) is one of the most important water soluble derivatives of chitosan. In this study, photo-sensitive azidehydroxyethyl chitosan (AZ-HECTS) was synthesized with grafting degree 3.4%, and its water solution resulted in an insoluble hydrogel by 254nm UV irradiation for 90s. AZ-HECTS hydrogels, with water absorption 86.21%, had little impact on growth of mouse fibroblast (L929) and presented good cell biocompatibility. Obvious sudden degradation stage, slow degradation stage and no apparent toxicity was observed after AZ-HECTS hydrogels implanted into rats, and tissue inflammation was slighter, which indicated favorable biological safety. Furthermore, AZ-HECTS hydrogels was loaded with heparin, and released 50% of heparin accumulated 14d. AZ-HECTS-heparin hydrogels showed inhibitory effects on L929, and pro-growth functions within vascular endothelial cells seeded on the hydrogels, meanwhile a positive influence on vascular endothelial growth factor expression. In conclusion, AZ-HECTS hydrogels possessed favorable biocompatibility and biodegradability and had good potential as drug carrier.
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: He
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Baoqin
authors.initials: B
authors.lastname: Han
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China. Electronic address: jiangzhiwen1987@163.com.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Wanshun
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology; D006493:Heparin / Q000008:administration & dosage; D020100:Hydrogels; D051381:Rats; D042461:Vascular Endothelial Growth Factor A
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D020100:Hydrogels; D042461:Vascular Endothelial Growth Factor A; D006493:Heparin; D048271:Chitosan
keywords: Biocompatibility; Biodegradability; Chitosan; Hydrogel; Photo-sensitive