title: In-Phase Ultra High-Resolution In Vivo NMR.
abstract: Although current NMR techniques allow organisms to be studied in vivo, magnetic susceptibility distortions, which arise from inhomogeneous distributions of chemical moieties, prevent the acquisition of high-resolution NMR spectra. Intermolecular single quantum coherence (iSQC) is a technique that breaks the sample's spatial isotropy to form long range dipolar couplings, which can be exploited to extract chemical shift information free of perturbations. While this approach holds vast potential, present practical limitations include radiation damping, relaxation losses, and non-phase sensitive data. Herein, these drawbacks are addressed, and a new technique termed in-phase iSQC (IP-iSQC) is introduced. When applied to a living system, high-resolution NMR spectra, nearly identical to a buffer extract, are obtained. The ability to look inside an organism and extract a high-resolution metabolic profile is profound and should find applications in fields in which metabolism or in vivo processes are of interest.
authors.affiliation: Dept. of Chemistry and Dept. Phys. Env. Sci., University of Toronto at Scarborough, 1265 Military Trail, Scarborough, ON, M1C 1A4, Canada.
authors.forename: Ioana
authors.initials: I
authors.lastname: Fugariu
authors.affiliation: Bruker Biospin GmbH, Silberstreifen 4, 76287, Rheinstetten, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bermel
authors.affiliation: Dept. of Chemistry and Dept. Phys. Env. Sci., University of Toronto at Scarborough, 1265 Military Trail, Scarborough, ON, M1C 1A4, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lane
authors.affiliation: Dept. of Chemistry and Dept. Phys. Env. Sci., University of Toronto at Scarborough, 1265 Military Trail, Scarborough, ON, M1C 1A4, Canada.
authors.forename: Ronald
authors.initials: R
authors.lastname: Soong
authors.affiliation: Dept. of Chemistry and Dept. Phys. Env. Sci., University of Toronto at Scarborough, 1265 Military Trail, Scarborough, ON, M1C 1A4, Canada.
authors.forename: Andre J
authors.initials: AJ
authors.lastname: Simpson
keywords: intermolecular single quantum coherence; in vivo NMR; magnetic susceptibilities; metabolic fingerprinting