title: Expression, Mutation, and Amplification Status of EGFR and Its Correlation with Five miRNAs in Salivary Gland Tumours.
abstract: Malignant salivary gland tumours are rare histologically and clinically heterogeneous group of tumours, missing prognostic factors and therapeutic targets. MicroRNAs (miRNAs), small noncoding RNAs, and posttranscriptional regulators of mRNA are poorly described in different subtypes of salivary gland tumours. Epidermal growth factor receptor (EGFR), an important therapeutic target and target of certain miRNAs (i.e.,miR-133b), shows variable degrees of expression in salivary gland tumours. Our study included 70 parotid gland tumours of different histological subtypes. Expression, mutations, and copy number variations (CNVs) of EGFR were determined using immunohistochemistry, single-stranded conformation polymorphism, quantitative polymerase chain reaction (qPCR), and fluorescence in situ hybridization. Expression ofmiR-99b,miR-133b,miR-140,miR-140-3p, andlet-7awas analysed using qPCR. Expression of EGFR was observed in 37% of tumours with low and 40% of tumours with high malignant potential. There were no mutations, with the majority of samples showing polysomy of chromosome 7. Based on histological subtypes, we found differential expression of all five miRNAs. We confirmed association of reactivity of EGFR,miR-133b,miR-140,miR-140-3p, andlet-7awith CNV of EGFR and a positive association betweenmiR-133b/let-7aand reactivity of EGFR. Age and need for postoperative radiotherapy were characterized as significant in multivariate survival analysis.
authors.affiliation: Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Zaloška Cesta 4, 1000 Ljubljana, Slovenia.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Boštjančič
authors.affiliation: Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Zaloška Cesta 4, 1000 Ljubljana, Slovenia.
authors.forename: Nina
authors.identifier: 0000-0002-7575-7716
authors.initials: N
authors.lastname: Hauptman
authors.affiliation: Department of Otorhinolaryngology and Cervicofacial Surgery, University Medical Centre Ljubljana, Zaloška Cesta 2, 1000 Ljubljana, Slovenia.
authors.forename: Aleš
authors.initials: A
authors.lastname: Grošelj
authors.affiliation: Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Zaloška Cesta 4, 1000 Ljubljana, Slovenia.
authors.forename: Damjan
authors.identifier: 0000-0001-9912-3331
authors.initials: D
authors.lastname: Glavač
authors.affiliation: Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia.
authors.forename: Metka
authors.identifier: 0000-0002-2878-4877
authors.initials: M
authors.lastname: Volavšek
mesh_terms: D000328:Adult; D000368:Aged; D056915:DNA Copy Number Variations / Q000235:genetics; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D012469:Salivary Glands / Q000378:metabolism / Q000473:pathology
chemical_list: C548234:MIRN133 microRNA, human; D035683:MicroRNAs; C544302:Mirn140 microRNA, human; C487931:mirnlet7 microRNA, human; C512478:EGFR protein, human; D066246:ErbB Receptors