title: A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis.
abstract: The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa247-370) was synthesized and cloned into pT1NX vector to construct the pT1NX-alpha plasmid. This surface-expressing plasmid was electroporated into Lactobacillus casei ATCC 393, generating the recombinant L. casei strain expressing alpha-toxoid (LC-α strain). Expression of this modified alpha-toxoid was confirmed by SDS-PAGE, immunoblotting, and direct immunofluorescence microscopy. BALB/c mice, immunized orally by the recombinant LC-α strain, elicited mucosal and significantly humoral immune responses (p < 0.05) and developed a protection against 900 MLD/mL of the standard alpha-toxin. This study showed that this recombinant LC-α strain could be a promising vaccine candidate against gas gangrene and necrotic enteritis.
authors.affiliation: Department of Molecular Microbiology, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.|Department of Anaerobic Bacterial Vaccine Research and Production, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.|Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, Kerman, 7636161313, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Alimolaei
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, Kerman, 7636161313, Iran. golchin@uk.ac.ir.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Golchin
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Abshenas
authors.affiliation: Department of Anaerobic Bacterial Vaccine Research and Production, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Ezatkhah
authors.affiliation: Department of Anaerobic Bacterial Vaccine Research and Production, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
authors.forename: Mehrdad Shamsaddini
authors.initials: MS
authors.lastname: Bafti
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000235:genetics / Q000276:immunology*; D004751:Enteritis / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005738:Gas Gangrene / Q000276:immunology / Q000517:prevention & control*; D015870:Gene Expression; D006801:Humans; D007114:Immunization; D007780:Lacticaseibacillus casei / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000008:administration & dosage*; D014121:Toxoids / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C035454:Clostridium perfringens toxoid; D014121:Toxoids
keywords: Clostridium perfringens; Lactobacillus casei; Probiotic; Toxoid; Vaccine