title: Roles of differential expression of microRNA-21-3p and microRNA-433 in FSH regulation in rat anterior pituitary cells.
abstract: Follicle-stimulating hormone (FSH) secreted by adenohypophyseal cells plays an important role in the regulation of reproduction, but whether microRNAs (miRNAs) regulate the secretion of FSH remains unclear. In the present study, we predicted and screened miRNAs that might act on the follicle-stimulating hormone beta-subunit (FSHb) gene of rats using the TargetScan program and luciferase reporter assays, and the results identified two miRNAs, miR-21-3p and miR-433. We then transfected these miRNAs into rat anterior adenohypophyseal cells and assessed the FSHb expression levels in and FSH secretion by the transfected cells through quantitative PCR and ELISA. The results showed that both miR-21-3p and miR-433 down-regulated the expression levels of FSHb and resulted in the decrease of the secretion of FSH compared with the control group, and treatment with miR-21-3p and miR-433 inhibitors up-regulated the expression levels of FSHb and resulted in the increase of the secretion of FSH. Taken together, our results indicate that miR-21-3p and miR-433 can down-regulate the expression of FSHb by directly targeting the FSHb 3'UTR in rat primary pituitary cells. Our findings provide evidence that miRNAs can regulate FSHb expression and further affect the secretion of FSH and might contribute to the use of miRNAs for the regulation of animal reproduction.
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Dong-Xu
authors.initials: DX
authors.lastname: Han
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Xu-Lei
authors.initials: XL
authors.lastname: Sun
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Ming-Qiang
authors.initials: MQ
authors.lastname: Xu
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Cheng-Zhen
authors.initials: CZ
authors.lastname: Chen
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Bao
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Jia-Bao
authors.initials: JB
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats; D015203:Reproducibility of Results; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C548883:MIRN433 microRNA, human; D035683:MicroRNAs; C546082:mirn21 microRNA, rat; D005640:Follicle Stimulating Hormone
keywords: FSH secretion; animal reproduction; miR-21-3p; miR-433; rat anterior pituitary cell