title: Isolation and identification of multiple drug resistant nontuberculous mycobacteria from organs of cattle produced typical granuloma lesions.
abstract: Nontuberculosis mycobacteria are widespread in the environment and some are zoonotic. 320 tissue samples with visible lesions were obtained from dairy cows and examined by histopathology. Eleven samples showed typical granulomatous lesions and a total of 8 strains were cultured. Three genes (16S rRNA, hsp65 and rpoB) were sequenced for species identification. All mycobacterial isolates were tested for rifampicin, isoniazid, ethambutol, streptomycin, capreomycin, kanamycin, para-aminosalicylic acid susceptibility. Six strains were identified as M. fortuitum, 1 was M. avium, 1 was M. conceptionense, isolated from cattle for the first time. Seven of the 8 isolated strains showed multiple drug resistance.
authors.affiliation: National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: hnchdd@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Cheng
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Tuberculosis Reference Laboratory, Changping, 100193 Beijing, China. Electronic address: xudonglei@icdc.cn.
authors.forename: Donglei
authors.initials: D
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018 Huhhot, China. Electronic address: wangjinlin-721@163.com.
authors.forename: Jinling
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: 75636301@qq.com.
authors.forename: Chunfa
authors.initials: C
authors.lastname: Liu
authors.affiliation: National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: 79554071@qq.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: 569057286@qq.com.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Tuberculosis Reference Laboratory, Changping, 100193 Beijing, China. Electronic address: liuhaican@icdc.cn.
authors.forename: Hancan
authors.initials: H
authors.lastname: Liu
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Tuberculosis Reference Laboratory, Changping, 100193 Beijing, China. Electronic address: wankanglin@icdc.cn.
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wan
authors.affiliation: National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: zhouxm@cau.edu.cn.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D018834:Chaperonin 60 / Q000235:genetics; D002681:China; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D006099:Granuloma / Q000382:microbiology* / Q000473:pathology; D008099:Liver / Q000473:pathology; D008826:Microbial Sensitivity Tests; D009164:Mycobacterium Infections / Q000382:microbiology* / Q000473:pathology; D009336:Necrosis / Q000382:microbiology / Q000473:pathology; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases
keywords: Drug resistance; Histopathology; M. fortuitum; Typical granulomatous lesions