title: Local application of statins in the treatment of experimental periodontal disease in rats.
abstract: OBJECTIVE
  The objective of this study was to evaluate the local effects of statins as adjuvants for treatment by scaling and root planing (SRP) of periodontal disease induced in rats.
  
  
  METHODS
  Ninety rats were used in the present experiment. Periodontal disease was induced in all animals using a cotton thread placed in the left first mandibular molar. After 7 days of induction, the bandage was removed and the animals were divided into three groups: 1) NT group (n=30), no treatment; 2) SRP group (n=30): SRP and irrigation with control gel; 3) S group (n=30) - SRP and irrigation with Simvastatin. Ten animals from each group were euthanized at 7, 15 and 30 days after treatment. Gingival biopsy specimens were processed to analyze the expression of matrix metalloproteinase 8 (MMP-8). The mandibles were removed and submitted to radiographic and laboratory processing for histometric analysis.
  
  
  RESULTS
  The S group showed a significantly lower expression of MMP-8 compared to NT and SRP groups in all experimental periods. In the radiographic and histometric analyses between the groups, S group showed a significantly lower bone loss (BL) compared to NT and SRP groups in all experimental periods.
  
  
  CONCLUSIONS
  Within the limits of this study, it can be concluded that locally applied statin was effective as an adjuvant treatment for SRP in rats with induced periodontal disease.
authors.affiliation: Universidade Federal de Alfenas, Faculdade de Odontologia, Alfenas, MG, Brasil.
authors.forename: Bianca Fernanda Espósito
authors.initials: BF
authors.lastname: Santos
authors.affiliation: Universidade Federal de Alfenas, Faculdade de Odontologia, Alfenas, MG, Brasil.
authors.forename: Eduardo Quintão Manhanini
authors.initials: EQ
authors.lastname: Souza
authors.affiliation: Universidade Federal de Alfenas, Faculdade de Odontologia, Departamento de Bioquímica, Alfenas, MG, Brasil.
authors.forename: Maísa Ribeiro Pereira Lima
authors.initials: MR
authors.lastname: Brigagão
authors.affiliation: Universidade Federal de Alfenas, Faculdade de Odontologia, Departamento de Clínica e Cirurgia, Alfenas, MG, Brasil.
authors.forename: Daniela Coelho de
authors.initials: DC
authors.lastname: Lima
authors.affiliation: Universidade Federal de Alfenas, Faculdade de Odontologia, Departamento de Clínica e Cirurgia, Alfenas, MG, Brasil.
authors.forename: Leandro Araújo
authors.initials: LA
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D001706:Biopsy; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D017024:Chemotherapy, Adjuvant; D005881:Gingiva / Q000187:drug effects / Q000473:pathology; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D010518:Periodontitis / Q000188:drug therapy* / Q000473:pathology; D017208:Rats, Wistar; D015203:Reproducibility of Results; D016745:Root Planing / Q000379:methods*; D019821:Simvastatin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D050071:Bone Density Conservation Agents; D019821:Simvastatin; D020784:Matrix Metalloproteinase 8; C000613587:Mmp8 protein, rat