title: Endo-siRNA deficiency results in oocyte maturation failure and apoptosis in porcine oocytes.
abstract: Both microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs) play key regulatory roles in gene expression. Some studies have demonstrated that the function of miRNA is suppressed in mouse oocytes, suggesting that endo-siRNA, not miRNA, is essential for female meiosis. This finding has yet to be confirmed in other species. In this study, by knockdown of DICER1, DROSHA and its cofactor DiGeorge syndrome critical region 8 (DGCR8) in porcine oocytes, we found that the proportion of oocytes with DICER1 deficiency that developed to meiosis II (MII) stage was significantly lower than oocytes with DROSHA and DGCR8 deficiency (39.23 versus 68.71 and 71.25% respectively; P<0.05). Oocytes lacking DROSHA and DGCR8 formed a barrel-shaped metaphase I spindle, with chromosomes tightly aligned at the metaphase plate whereas most oocytes (87%) lacking DICER1 showed spindle abnormalities during oocyte in vitro maturation. Furthermore, DICER1 deficiency also resulted in oocyte apoptosis. These results indicate that endo-siRNAs are essential for oocyte maturation in pigs.
authors.affiliation: Laboratory of Animal Developmental Biology, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China.
authors.forename: Wanxin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Laboratory of Animal Developmental Biology, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Laboratory of Animal Developmental Biology, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China.
authors.forename: Shanhua
authors.initials: S
authors.lastname: Piao
authors.affiliation: Laboratory of Animal Developmental Biology, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Laboratory of Embryo Biotechnology, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China.
authors.forename: Qingran
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Laboratory of Animal Developmental Biology, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang Province 150040, China.
authors.forename: Tiezhu
authors.initials: T
authors.lastname: An
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D008540:Meiosis / Q000502:physiology; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases