title: Experimental infections of wild bank voles (Myodes glareolus) from nephropatia epidemica endemic and non-endemic regions revealed slight differences in Puumala virological course and immunological responses.
abstract: In Europe, the occurrence of nephropathia epidemica (NE), a human disease caused by Puumala virus (PUUV), exhibits considerable geographical heterogeneity despite the continuous distribution of its reservoir, the bank vole Myodes glareolus. To better understand the causes of this heterogeneity, wild voles sampled in two adjacent NE endemic and non-endemic regions of France were infected experimentally with PUUV. The responses of bank voles to PUUV infection, based on the levels of anti-PUUV IgG and viral RNA, were compared. Slight regional differences were highlighted despite the high inter-individual variability. Voles from the NE non-endemic region showed greater immune responsiveness to PUUV infection, but lower levels of RNA in their organs than voles from the endemic region. These results suggest the existence of regional variations in the sensitivity of bank voles that could contribute to the apparent absence of PUUV circulation among voles and the absence of NE in the non-endemic region.
authors.affiliation: INRA, Centre de Biologie pour la Gestion des Populations, Montferrier sur Lez, France; ANSES, Unité de Virologie, Laboratoire de Lyon, France. Electronic address: adelaide.dubois@inra.fr.
authors.forename: Adélaïde
authors.initials: A
authors.lastname: Dubois
authors.affiliation: INRA, Centre de Biologie pour la Gestion des Populations, Montferrier sur Lez, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Castel
authors.affiliation: ANSES, Unité de Virologie, Laboratoire de Lyon, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Murri
authors.affiliation: ANSES, Plateforme d'Expérimentation Animale, Laboratoire de Lyon, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Pulido
authors.affiliation: CNRS, Laboratoire de Biométrie et Biologie Evolutive, Villeurbanne, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Pons
authors.affiliation: INRA, Centre de Biologie pour la Gestion des Populations, Montferrier sur Lez, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Benoit
authors.affiliation: INRA, Centre de Biologie pour la Gestion des Populations, Montferrier sur Lez, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Loiseau
authors.affiliation: ANSES, Plateforme d'Expérimentation Animale, Laboratoire de Lyon, France.
authors.forename: Latifa
authors.initials: L
authors.lastname: Lakhdar
authors.affiliation: INRA, Centre de Biologie pour la Gestion des Populations, Montferrier sur Lez, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Galan
authors.affiliation: INRA, Centre de Biologie pour la Gestion des Populations, Montferrier sur Lez, France. Electronic address: nathalie.charbonnel@inra.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Charbonnel
authors.affiliation: ANSES, Unité de Virologie, Laboratoire de Lyon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Marianneau
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003411:Arvicolinae; D004197:Disease Reservoirs; D004198:Disease Susceptibility; D005602:France; D006480:Hemorrhagic Fever with Renal Syndrome / Q000473:pathology / Q000821:virology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D029262:Puumala virus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D012367:RNA, Viral / Q000097:blood; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral
keywords: Experimental infections; Immunology; Myodes glareolus; Nephropathia epidemica; Puumala virus; Wild bank vole