title: Anti-obesity effect of a novel caffeine-loaded dissolving microneedle patch in high-fat diet-induced obese C57BL/6J mice.
abstract: Natural products such as caffeine have been found to be effective in reducing body weight through lipolysis. Here, we report the successful loading of caffeine onto dissolving microneedle following inhibition of its crystal growth by hyaluronic acid (HA), the matrix material of the dissolving microneedle (DMN). Further, the anti-obesity activity of caffeine was evaluated in high-fat diet-induced obese C57BL/6J mice. After 6weeks of caffeine loaded dissolving microneedle patch (CMP) administration, lipolysis improved significantly as shown by leptin and adiponectin activity, which resulted in considerable weight loss of about 12.8±0.75% in high-fat diet-induced obese mice. Comparison of the levels of triglyceride, total cholesterol, high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol after CMP administration with the initial levels in obese mice indicated significant anti-obesity activity of CMP. These findings suggested that a novel CMP with an increased amount of caffeine loaded onto DMN has therapeutic activity against obesity.
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Manita
authors.initials: M
authors.lastname: Dangol
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; Juvic Biotech, Inc., Building 102, Yonsei Engineering Research Park, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Suyong
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Cheng Guo
authors.initials: CG
authors.lastname: Li
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Shayan
authors.initials: S
authors.lastname: Fakhraei Lahiji
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; Juvic Biotech, Inc., Building 102, Yonsei Engineering Research Park, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Jang
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Yonghao
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Inyoung
authors.initials: I
authors.lastname: Huh
authors.affiliation: Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; Juvic Biotech, Inc., Building 102, Yonsei Engineering Research Park, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea. Electronic address: hijung@yonsei.ac.kr.
authors.forename: Hyungil
authors.initials: H
authors.lastname: Jung
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002110:Caffeine / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival; D002784:Cholesterol / Q000378:metabolism; D059305:Diet, High-Fat; D016503:Drug Delivery Systems / Q000379:methods; D005079:Excipients; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000378:metabolism; D008422:Materials Testing / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008845:Microinjections; D009339:Needles; D009765:Obesity / Q000188:drug therapy*; D011108:Polymers / Q000737:chemistry; D012867:Skin / Q000378:metabolism; D057968:Transdermal Patch; D014280:Triglycerides / Q000378:metabolism; D015431:Weight Loss
chemical_list: D019440:Anti-Obesity Agents; D005079:Excipients; D008075:Lipoproteins, HDL; D011108:Polymers; D014280:Triglycerides; D002110:Caffeine; D006820:Hyaluronic Acid; D002784:Cholesterol
keywords: Anti-obesity; Caffeine; Crystal growth; Diet induced obesity; Weight loss