title: Association of IL-1β, IL-1Ra and FABP1 gene polymorphisms with the metabolic features of polycystic ovary syndrome.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS), a highly prevalent endocrinopathy is currently being designated as chronic low grade inflammatory state. IL-1β, IL-1Ra and FABP1 are critical mediators of inflammatory processes and are speculated to play a role in the pathogenesis of PCOS. The aim of this study was to study the association of IL-β, IL-1Ra and FABP1 gene polymorphisms with PCOS and related metabolic features.
  
  
  METHODS
  95 PCOS and 45 age matched healthy control subjects were enrolled in this study.
  
  
  METHODS
  Polymorphism in genes IL-1β, IL-1Ra and FABP1 was studied by PCR, PCR-RFLP and sequencing methods, respectively. Hormonal and lipid profiles were evaluated for all the subjects.
  
  
  RESULTS
  Hormonal and lipid profiles showed significant differences between PCOS and control subjects. Allele and genotype frequencies of IL-1β, IL-1Ra and FABP1 gene polymorphisms did not vary between the control and PCOS group. However, T allele of C[-511]T variant of IL-1β, allele II in intron 2 of IL-1Ra and A allele of A/G variant of FABP1 (rs2197076) showed significant association with many metabolic features associated with PCOS.
  
  
  CONCLUSIONS
  Polymorphism in genes encoding cytokines and proteins involved in lipid metabolism can provide insights into the genetics of the disease and may contribute to assess the associated risk of cardiovascular diseases (CVD), dyslipidemia and metabolic syndrome (MetS) associated with PCOS.
authors.affiliation: Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard (Hamdard University), New Delhi, 110062, India.
authors.forename: Nadia
authors.initials: N
authors.lastname: Rashid
authors.affiliation: Department of Gynaecology and Obstetrics, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard (Hamdard University), New Delhi, 110062, India.
authors.forename: Aruna
authors.initials: A
authors.lastname: Nigam
authors.affiliation: Department of Obstetrics and Gynaecology, Lady Hardinge Medical College and SSK Hospital, New Delhi, 110001, India.
authors.forename: Pikee
authors.initials: P
authors.lastname: Saxena
authors.affiliation: Department of Biochemistry, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard (Hamdard University), New Delhi, 110062, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Jain
authors.affiliation: Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard (Hamdard University), New Delhi, 110062, India. swajid@jamiahamdard.ac.in.
authors.forename: Saima
authors.initials: S
authors.lastname: Wajid
mesh_terms: D000328:Adult; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; C508585:IL1B protein, human; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta
keywords: FABP1; IL-1Ra; IL-1β; Metabolic syndrome; PCOS