title: Cerium Oxide Nanoparticles in Lung Acutely Induce Oxidative Stress, Inflammation, and DNA Damage in Various Organs of Mice.
abstract: CeO2nanoparticles (CeO2NPs) which are used as a diesel fuel additive are emitted in the particulate phase in the exhaust, posing a health concern. However, limited information exists regarding thein vivoacute toxicity of CeO2NPs on multiple organs. Presently, we investigated the acute (24 h) effects of intratracheally instilled CeO2NPs in mice (0.5 mg/kg) on oxidative stress, inflammation, and DNA damage in major organs including lung, heart, liver, kidneys, spleen, and brain. Lipid peroxidation measured by malondialdehyde production was increased in the lungs only, and reactive oxygen species were increased in the lung, heart, kidney, and brain. Superoxide dismutase activity was decreased in the lung, liver, and kidney, whereas glutathione increased in lung but it decreased in the kidney. Total nitric oxide was increased in the lung and spleen but it decreased in the heart. Tumour necrosis factor-αincreased in all organs studied. Interleukin- (IL-) 6 increased in the lung, heart, liver, kidney, and spleen. IL-1βaugmented in the lung, heart, kidney, and spleen. Moreover, CeO2NPs induced DNA damage, assessed by COMET assay, in all organs studied. Collectively, these findings indicate that pulmonary exposure to CeO2NPs causes oxidative stress, inflammation, and DNA damage in multiple organs.
authors.affiliation: Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Abderrahim
authors.identifier: 0000-0002-0699-1015
authors.initials: A
authors.lastname: Nemmar
authors.affiliation: Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Priya
authors.initials: P
authors.lastname: Yuvaraju
authors.affiliation: Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Sumaya
authors.initials: S
authors.lastname: Beegam
authors.affiliation: Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Fahim
authors.affiliation: Department of Pharmacology, College of Medicine & Health Sciences, Sultan Qaboos University, P.O. Box 35, Al-Khod, 123 Muscat, Oman.
authors.forename: Badreldin H
authors.initials: BH
authors.lastname: Ali
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000503:physiopathology; D002563:Cerium / Q000633:toxicity*; D004249:DNA Damage; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D007249:Inflammation / Q000139:chemically induced; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000503:physiopathology; D008168:Lung / Q000187:drug effects* / Q000503:physiopathology*; D051379:Mice; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress; D052638:Particulate Matter / Q000633:toxicity; D013154:Spleen / Q000187:drug effects / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D052638:Particulate Matter; D002563:Cerium; C030583:ceric oxide; D013482:Superoxide Dismutase