title: Modelling apical columnar epithelium mechanics from circumferential contractile fibres.
abstract: Simple columnar epithelia are formed by individual epithelial cells connecting together to form single cell high sheets. They are a main component of many important body tissues and are heavily involved in both normal and cancerous cell activities. Prior experimental observations have identified a series of contractile fibres around the circumference of a cross section located in the upper (apical) region of each cell. While other potential mechanisms have been identified in both the experimental and theoretical literature, these circumferential fibres are considered to be the most likely mechanism controlling movement of this cross section. Here, we investigated the impact of circumferential contractile fibres on movement of the cross section by creating an alternate model where movement is driven from circumferential contractile fibres, without any other potential mechanisms. In this model, we utilised a circumferential contractile fibre representation based on investigations into the movement of contractile fibres as an individual system, treated circumferential fibres as a series of units, and matched our model simulation to experimental geometries. By testing against laser ablation datasets sourced from existing literature, we found that circumferential fibres can reproduce the majority of cross-sectional movements. We also investigated model predictions related to various aspects of cross-sectional movement, providing insights into epithelium mechanics and demonstrating the usefulness of our modelling approach.
authors.affiliation: Department of Mechanical Engineering, University of Melbourne, Melbourne, VIC, 3010, Australia.
authors.forename: A R B
authors.identifier: http://orcid.org/0000-0002-0462-5767
authors.initials: ARB
authors.lastname: Boyd
authors.affiliation: IBM Research Australia, Level 5, 204 Lygon Street, Carlton, VIC, 3010, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Moore
authors.affiliation: School of Mathematics and Statistics, University of Melbourne, Melbourne, VIC, 3010, Australia.
authors.forename: J E
authors.initials: JE
authors.lastname: Sader
authors.affiliation: Department of Mechanical Engineering, University of Melbourne, Melbourne, VIC, 3010, Australia. pvlee@unimelb.edu.au.
authors.forename: P V S
authors.identifier: http://orcid.org/0000-0003-3666-4872
authors.initials: PVS
authors.lastname: Lee
mesh_terms: D001696:Biomechanical Phenomena; D004848:Epithelium / Q000502:physiology*; D053685:Laser Therapy; D008954:Models, Biological
keywords: Columnar epithelium; Computational biomechanics; Drosophila; Laser ablation; Mechanistic modelling; Vertex model