title: Serum and exosomal miR-122 and miR-199a as a biomarker to predict therapeutic efficacy of hepatitis C patients.
abstract: MicroRNA (miRNA), which has been shown to correlate with liver functions, has been proposed as a new biomarker reflecting liver injury. The aim of the study was to investigate miRNA-122 (miR-122) and mir-RNA-199a (miR-199a) as a biomarker for predicting therapeutic efficacy in hepatitis C (HepC) patients. A total of 47 HepC 1b patients and 16 healthy subjects were enrolled in the study. Serum and exosomal mir-RNAs and other conventional biomarkers reflecting liver function were evaluated. The miR-122 levels in serum (miR-122ser) and exosomes (miR-122exo) were significantly lower in the Hepatitis C virus (HCV) genotype 1b patients than in the normal controls, but these levels were higher compared to the non-genotype 1b group. The mean miR-122serlevel in the sustained virological response (SVR) group was significantly higher than that in the non-response (NR) group (P < 0.01), and the miR-122exolevel in the SVR group was also higher than that in the NR group (P > 0.05), although this difference was not significant. miR-199a levels showed similar trends with the miR-122 levels in serum and exosomes. HCV RNAserwas negatively correlated with the miR-122ser(r = -0.473, P = 0.004) and miR-122exo(r = -0.424, P = 0.009) levels. miR-122serlevels were positively associated with miR-199aserlevels (r = 0.453, P = 0.002).Univariate and multivariate regression analyses reveal that the miR-122serlevels and ALT/AST ratio demonstrated a predictive value in evaluating patient outcomes. Serum miR-122 and miR-199a are potential biomarkers that reflect therapeutic efficacy.
authors.affiliation: Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.
authors.forename: Xiaoyang
authors.identifier: 0000-0002-7568-0595
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.|Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine, Second Affiliated Hospital of Henan University of TCM, Henan, P.R. China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.
authors.forename: Huanzhu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, P.R. China.
authors.forename: Qiaoxin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Cell Biology and Genetics Department, Shantou University Medical College, Shantou, Guangdong, P.R. China.|Center for Disease Control and Prevention of Guangdong Province, Guangzhou.
authors.forename: Changwen
authors.initials: C
authors.lastname: Ke
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D016903:Drug Monitoring / Q000379:methods*; D055354:Exosomes / Q000737:chemistry*; D005260:Female; D019698:Hepatitis C, Chronic / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011237:Predictive Value of Tests; D044967:Serum / Q000737:chemistry*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; C531408:mirn199 microRNA, human
keywords: HCV; exosome; miR-122; miR-199a