title: Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis).
abstract: Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and characterized. The full-length cDNA of aqp1aa is 1411 bp with a 786 bp open reading frame encoding a 261-amino acid putative protein with a characteristic structure consisting of 6 membrane-spanning α-helical domains and two highly conserved asparagine-proline-alanine motifs. Real-time quantitative PCR revealed that aqp1aa mRNA is expressed predominantly in the testis of males and pseudo-males, while its expression is low in the ovary and lowest in doublesex and mab-3-related transcription factor 1(DMRT1) knock out fish and triploid males. In situ hybridization indicated that aqp1aa mRNA is expressed mainly in the germ cells of males and pseudo-males, especially in spermatozoa and spermatids. These results suggest that the aqp1aa may play a role in spermatogenesis of C. semilaevis.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|College of Fisheries and Life, Shanghai Ocean University, Shanghai, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Guo
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Wenteng
authors.initials: W
authors.lastname: Xu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Meng
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Shao
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Lu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.
authors.forename: Fengtao
authors.initials: F
authors.lastname: Gao
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|College of Fisheries and Life, Shanghai Ocean University, Shanghai, PR China.
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|College of Fisheries and Life, Shanghai Ocean University, Shanghai, PR China.
authors.forename: Zhanfei
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, PR China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: Fazhen
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, PR China.|College of Fisheries and Life, Shanghai Ocean University, Shanghai, PR China.|Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, PR China.|Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
authors.forename: Songlin
authors.identifier: http://orcid.org/0000-0002-7007-0640
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D005412:Flatfishes / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008297:Male; D010053:Ovary / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000378:metabolism
chemical_list: D051398:Aquaporin 1