title: Methylation on RNA: A Potential Mechanism Related to Immune Priming within But Not across Generations.
abstract: Invertebrate immune priming is a growing field in immunology. This phenomenon refers to the ability of invertebrates to generate a more vigorous immune response to a second encounter with a specific pathogen and can occur within and across generations. Although the precise mechanism has not been elucidated, it has been suggested that methylation of DNA is a cornerstone for this phenomenon. Here, using a novel method of analytical chemistry (a reversed-phase liquid chromatography procedure) and the beetleTenebrio molitoras a model system, we did not find evidence to support this hypothesis taking into account the percentage of methylated cytosine entities in DNA (5mdC) within or across generations. However, we found a lower percentage of methylated cytosine entities in RNA (5mC) within but not across generations in immune priming experiments with adults against the bacteriaMicrococcus lysodeikticusand larvae against the fungusMetarhizium anisopliae. To our knowledge, this is the first report suggesting a role of differential methylation on RNA during immune priming within generations.
authors.affiliation: Departamento de Biología, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Castro-Vargas
authors.affiliation: Departamento de Biología, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: César
authors.initials: C
authors.lastname: Linares-López
authors.affiliation: Departamento de Química, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: Adolfo
authors.initials: A
authors.lastname: López-Torres
authors.affiliation: Departamento de Química, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wrobel
authors.affiliation: Departamento de Biología, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Torres-Guzmán
authors.affiliation: Departamento de Biología, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: Gloria A G
authors.initials: GA
authors.lastname: Hernández
authors.affiliation: Departamento de Química, Universidad de Guanajuato Guanajuato, Mexico.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Wrobel
authors.affiliation: Instituto Nacional de Salud Pública Cuernavaca, Mexico.
authors.forename: Humberto
authors.initials: H
authors.lastname: Lanz-Mendoza
authors.affiliation: ENES, Unidad Morelia, Universidad Nacional Autónoma de México Morelia, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Contreras-Garduño
keywords: DNA; RNA; Tenebrio molitor; epigenetics; high-performance liquid chromatography; immune priming; innate immune memory; methylation