title: DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo.
abstract: Dendritic cells (DCs) are efficient antigen-presenting cells equipped with various cell surface receptors for the direct or indirect recognition of pathogenic microorganisms. Interestingly, not much is known about the specific expression pattern and function of the individual activating and inhibitory Fcγ receptors (FcγRs) on splenic DC subsets in vivo and how they contribute to the initiation of T cell responses. By targeting antigens to select activating and the inhibitory FcγR in vivo, we show that antigen uptake under steady-state conditions results in a short-term expansion of antigen-specific T cells, whereas under inflammatory conditions especially, the activating FcγRIV is able to induce superior CD4+and CD8+T cell responses. Of note, this effect was independent of FcγR intrinsic activating signaling pathways. Moreover, despite the expression of FcγRIV on both conventional splenic DC subsets, the induction of CD8+T cell responses was largely dependent on CD11c+CD8+DCs, whereas CD11c+CD8-DCs were critical for priming CD4+T cell responses.
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Christian H K
authors.identifier: 0000-0001-5927-9761
authors.initials: CHK
authors.lastname: Lehmann
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.|Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Institut National de la Santé et de la Recherche Médicale-Centre National de la Recherche Scientifique, 13288 Marseille-Luminy, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Baranska
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Gordon F
authors.identifier: 0000-0002-0197-1031
authors.initials: GF
authors.lastname: Heidkamp
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Lukas
authors.identifier: 0000-0001-5591-2187
authors.initials: L
authors.lastname: Heger
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Neubert
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Jennifer J
authors.identifier: 0000-0001-6927-0867
authors.initials: JJ
authors.lastname: Lühr
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Alana
authors.identifier: 0000-0002-5476-1038
authors.initials: A
authors.lastname: Hoffmann
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Katharina C
authors.identifier: 0000-0002-7118-9133
authors.initials: KC
authors.lastname: Reimer
authors.affiliation: Department of Biology, Chair of Genetics, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Christin
authors.identifier: 0000-0002-2463-5436
authors.initials: C
authors.lastname: Brückner
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Beck
authors.affiliation: Department of Biology, Chair of Genetics, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Michaela
authors.identifier: 0000-0002-4788-4021
authors.initials: M
authors.lastname: Seeling
authors.affiliation: Department of Biology, Chair of Genetics, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Melissa
authors.initials: M
authors.lastname: Kießling
authors.affiliation: Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Didier
authors.identifier: 0000-0002-3688-5846
authors.initials: D
authors.lastname: Soulat
authors.affiliation: Institute for Immunology, Biomedical Center, Ludwig-Maximilians-University Munich, 82152 Planegg-Martinsried, Germany.
authors.forename: Anne B
authors.initials: AB
authors.lastname: Krug
authors.affiliation: Leonard Wagner Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York, NY 10065.
authors.forename: Jeffrey V
authors.initials: JV
authors.lastname: Ravetch
authors.affiliation: Immunotherapy Laboratory, Laboratory for Translational Immunology, University Medical Center Utrecht, 3584 Utrecht, Netherlands.
authors.forename: Jeanette H W
authors.identifier: 0000-0003-4982-6914
authors.initials: JHW
authors.lastname: Leusen
authors.affiliation: Department of Biology, Chair of Genetics, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany diana.dudziak@uk-erlangen.de falk.nimmerjahn@fau.de.|Medical Immunology Campus Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Falk
authors.initials: F
authors.lastname: Nimmerjahn
authors.affiliation: Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital of Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany diana.dudziak@uk-erlangen.de falk.nimmerjahn@fau.de.|Medical Immunology Campus Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Diana
authors.identifier: 0000-0001-9358-134X
authors.initials: D
authors.lastname: Dudziak
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D017452:Receptors, IgG / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D011948:Receptors, Antigen, T-Cell; D017452:Receptors, IgG