title: An Open-Source Automated Peptide Synthesizer Based on Arduino and Python.
abstract: The development of the first open-source automated peptide synthesizer, PepSy, using Arduino UNO and readily available components is reported. PepSy was primarily designed to synthesize small peptides in a relatively small scale (<100 µmol). Scripts to operate PepSy in a fully automatic or manual mode were written in Python. Fully automatic script includes functions to carry out resin swelling, resin washing, single coupling, double coupling, Fmoc deprotection, ivDde deprotection, on-resin oxidation, end capping, and amino acid/reagent line cleaning. Several small peptides and peptide conjugates were successfully synthesized on PepSy with reasonably good yields and purity depending on the complexity of the peptide.
authors.affiliation: 1 Department of Pharmaceutical Sciences, College of Pharmacy, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Hariprasad
authors.initials: H
authors.lastname: Gali
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods; D060326:Chemistry Techniques, Synthetic / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis*
chemical_list: D010455:Peptides
keywords: Arduino; Python; SPPS; do-it-yourself; laboratory automation; peptide synthesizer