title: Establishment of preliminary regulatory network of TRPV1 and related cytokines.
abstract: Our purpose was to investigate the regulatory mechanism of TRPV1 and related cytokines on children bronchial asthma. TRPV1 mRNA level and two SNP genotypes of children in case group and control group were detected by real-time quantitative PCR. Western blot and ELISA were used to measure the levels of cytokines like IgE, IL-2, etc. Their correlations were analyzed by Logistic regression and KEGG analysis. Moreover, tertiary structure of protein and miRNA binding sites were also predicted by online tools. Case group was obviously different from control group in TRPV1 mRNA level, the two SNP genotypes distribution and the related cytokines levels. Logistic regression analysis further demonstrated that TRPV1 mRNA level, EOS, IL-4 and IL-5 may be risk factors for children bronchial asthma. And based on that, the preliminary regulatory network of children bronchial asthma was drawn. What's more, mutation of rs4790521 and rs4790522 in TRPV1 gene both induced its corresponding miRNA binding site's change. The preliminary regulatory network of TRPV1 and related cytokines on children bronchial asthma established in this study provides certain theoretical basis for pathogenesis and treatment of children bronchial asthma.
authors.affiliation: Medical Engineering Technology and Data Mining Institute of Zhengzhou University, No. 100 Science Ave., Gaoxin Dist., Zhengzhou 450001, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiration, The Second Affiliated Hospital of Zhengzhou University, No. 2 Jingba Rd., Zhengzhou 450014, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Medical Engineering Technology and Data Mining Institute of Zhengzhou University, No. 100 Science Ave., Gaoxin Dist., Zhengzhou 450001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Medical Engineering Technology and Data Mining Institute of Zhengzhou University, No. 100 Science Ave., Gaoxin Dist., Zhengzhou 450001, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Jin
authors.affiliation: Department of Chinese Internal Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 East Jianshe Rd., Zhengzhou 450052, China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Hospital Office, People's Hospital of Zhengzhou University, No. 7 Weiwu Rd., Zhengzhou 450003, China.
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Chen
keywords: Children bronchial asthma; Cytokines; Molecular mechanism; Polymorphis; Preliminary regulatory network; TRPV1; miRNA