title: An injectable nucleus pulposus cell-modified decellularized scaffold: biocompatible material for prevention of disc degeneration.
abstract: We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix. The xenogeneic decellularized SIS did not display cytotoxicity in vitro and its application prevented NPC degradation. Furthermore, the xenogeneic SIS microparticles were effective in preventing IVD progression in vivo in a rabbit disc degeneration model. In conclusion, our study describes an optimized method for decellularized SIS preparation and demonstrated that the material is safe and effective for treating IVD degeneration.
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Shan
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Macromolecular synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Yichuan
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Shengyun
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Tianming
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Shunwu
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
authors.forename: Fengdong
authors.initials: F
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007403:Intervertebral Disc / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000517:prevention & control* / Q000628:therapy*; D007413:Intestinal Mucosa / Q000166:cytology; D017116:Low Back Pain / Q000628:therapy; D000070614:Nucleus Pulposus / Q000166:cytology*; D011817:Rabbits; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials
keywords: decellularization; extracellular matrix; intervertebral disc degeneration; nucleus pulposus cell; small intestinal submucosa