title: Bicomponent fibrous scaffolds made through dual-source dual-power electrospinning: Dual delivery of rhBMP-2 and Ca-P nanoparticles and enhanced biological performances.
abstract: Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning. The release behavior of rhBMP-2 and Ca2+ions could be separately tuned and the released rhBMP-2 retained a 68% level for biological activity. MC3T3-E1 cells showed high viability and normal morphology on scaffolds. Compared to monocomponent scaffolds, enhanced cell proliferation, alkaline phosphatase activity, cell mineralization, and gene expression of osteogenic markers were achieved for bicomponent scaffolds due to the synergistic effect of rhBMP-2 and Ca-P nanoparticles. Bicomponent scaffolds with a double mass elicited further enhanced cell adhesion, spreading, proliferation, and osteogenic differentiation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2199-2209, 2017.
authors.affiliation: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Orthopedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sasson Road, Hong Kong.
authors.forename: William Weijia
authors.initials: WW
authors.lastname: Lu
authors.affiliation: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D053758:Nanoparticles / Q000008:administration & dosage*; D010012:Osteogenesis / Q000187:drug effects; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2
keywords: Ca-P nanoparticle; bicomponent scaffold; bone tissue engineering; dual-source dual-power electrospinning; rhBMP-2