title: Intraspecies comparative genomics of three strains ofOrientia tsutsugamushiwith different antibiotic sensitivity.
abstract: We recently reported the genome ofOrientia tsutsugamushi(OT) strain Karp (GenBank Accession #: NZ_LYMA00000000.2, https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2) with > 2 Mb in size through clone-based sequencing and high throughput genomic shotgun sequencing (HTS). The genomes of OT strains AFSC4 and AFSC7 were similarly sequenced by HTS Since strains AFSC4 (GenBank Accession #: NZ_LYMT00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035784408) and AFSC7 (GenBank Accession #: NZ_LYMB00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035854767) were more resistant to antibiotics than strain Karp, we conducted comparative analysis of the three draft genomes annotated by RAST server aimed to identify possible genetic bases of difference in microbial antibiotic sensitivity. Intraspecies comparative genomics analysis of the three OT strains revealed that two ORFs encoding hypothetical proteins in both strains AFSC4 and AFSC7 are absent in strain Karp.
authors.affiliation: Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Hsiao-Mei
authors.initials: HM
authors.lastname: Liao
authors.affiliation: Naval Medical Research Center, Silver Spring, MD, USA; Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Chao
authors.affiliation: Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lei
authors.affiliation: Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Li
authors.affiliation: Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Shien
authors.initials: S
authors.lastname: Tsai
authors.affiliation: Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Guo-Chiuan
authors.initials: GC
authors.lastname: Hung
authors.affiliation: Naval Medical Research Center, Silver Spring, MD, USA; Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
authors.forename: Wei-Mei
authors.initials: WM
authors.lastname: Ching
authors.affiliation: Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Shyh-Ching
authors.initials: SC
authors.lastname: Lo
keywords: Antibiotic sensitivity; Comparative genomic analysis; Gene function comparison; Genome sequence comparison; Orientia tsutsugamushi; RAST genome annotation