title: Characterization of oligonucleotide aptamers targeting the 5'-UTR from dengue virus.
abstract: The dengue virus is responsible for a high worldwide incidence of infections, aggravated by late diagnosis, and often confused with other tropical diseases. Results/methodology: Oligonucleotide aptamers binding to the 5'-UTR from dengue virus selected after eight rounds by systematic evolution of ligands by exponential enrichment technology were analyzed by dot-blot assay and in silico prediction of secondary structures, demonstrating the presence of stem-loops that may have the potential for interaction with the viral genome, which can lead to loss of their original conformation.
  
  
  
  This is the first description of RNA aptamers against functional RNA elements of the dengue virus genome with implications for disease control, which may have potential as tools in the future of antiviral therapies and for diagnostics.
authors.affiliation: Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.
authors.forename: Elismar Jn
authors.initials: EJ
authors.lastname: Cnossen
authors.affiliation: Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.
authors.forename: Amanda G
authors.initials: AG
authors.lastname: Silva
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics & Biochemistry, Universidade Federal de Uberlandia, Uberlandia, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Marangoni
authors.affiliation: Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.
authors.forename: Rívia Ar
authors.initials: RA
authors.lastname: Arruda
authors.affiliation: Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.
authors.forename: Evellyn G
authors.initials: EG
authors.lastname: Souza
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics & Biochemistry, Universidade Federal de Uberlandia, Uberlandia, Brazil.
authors.forename: Fabiana Aa
authors.initials: FA
authors.lastname: Santos
authors.affiliation: Laboratory of Nanobiotechnology, Institute of Genetics & Biochemistry, Universidade Federal de Uberlandia, Uberlandia, Brazil.
authors.forename: Patrícia T
authors.initials: PT
authors.lastname: Fujimura
authors.affiliation: Laboratory of Virology, Institute of Biomedical Sciences, Universidade Federal de Uberlandia, Uberlandia, Brazil.
authors.forename: Jonny
authors.initials: J
authors.lastname: Yokosawa
authors.affiliation: Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.|Department of Medical Microbiology & Immunology, University of California Davis, Davis, CL 95616, USA.
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Goulart
authors.affiliation: Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Neves
mesh_terms: D020121:5' Untranslated Regions / Q000187:drug effects* / Q000235:genetics; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D003716:Dengue Virus / Q000187:drug effects* / Q000235:genetics; D008024:Ligands; D008826:Microbial Sensitivity Tests; D009841:Oligonucleotides / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D020121:5' Untranslated Regions; D000998:Antiviral Agents; D052157:Aptamers, Nucleotide; D008024:Ligands; D009841:Oligonucleotides
keywords: SELEX; dot-blot; polymerase chain reaction; reverse transcription; untranslated region