title: A randomized controlled study on the efficacy of a novel combination vaccine against enzootic pneumonia (Mycoplasma hyopneumoniae) and porcine Circovirus type 2 (PCV2) in the presence of strong maternally derived PCV2 immunity in pigs.
abstract: BACKGROUND
  Mycoplasma hyopneumoniae (M. hyo) and Porcine Circovirus Type 2 (PCV2) are major pathogens that cause significant health problems in swine worldwide. Maternal derived immunity (MDI) has been suggested as a significant immediate defence factor for newborn piglets and may interfere with piglet's vaccination-induced immunity. The study aimed to assess the efficacy of a novel combination vaccine (consisting of PCV2 subunits and inactivated M. hyo strain J), against PCV2 and M. hyo natural infection [Porcilis®PCV M Hyo (MSD Animal Health, Boxmeer, the Netherlands)], in the presence of strong maternally derived PCV2 immunity (antibody titre averaged 11.08 log2), under field conditions. The study was performed according to a controlled, randomized and blinded design in a Greek swine unit with Enzootic Pneumonia (EP) and subclinical PCV2 infection. In total, 600 healthy three-week-old suckling piglets were allocated randomly, either to treatment (vaccinated with the test product) or control group (injected with sterile buffered saline).
  
  
  RESULTS
  Vaccination significantly reduced the severity of lung lesions at slaughter (lesions of cranio-ventral pulmonary consolidation) (P < 0.001). The overall mean lung lesion score (LLS) was 9.6 in the vaccinated group and 12.2 in controls. The level of PCV2 viraemia was significantly reduced in vaccinated pigs. Furthermore, 25 g higher average daily weight gain (ADWG) was observed during the finishing phase (P < 0.001) and 18 g greater ADWG overall (P < 0.001).
  
  
  CONCLUSIONS
  Results of LLS, PCV2 viremia and ADWG support the test product's efficacy in the face of strong maternally derived PCV2 immunity.
authors.affiliation: Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 54627, Thessaloniki, Greece. ptassis@vet.auth.gr.
authors.forename: Panagiotis D
authors.initials: PD
authors.lastname: Tassis
authors.affiliation: Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 54627, Thessaloniki, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Tsakmakidis
authors.affiliation: Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, P.O. Box 199, Trikalon 224, Karditsa, Greece.
authors.forename: Vassileios G
authors.initials: VG
authors.lastname: Papatsiros
authors.affiliation: Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 54627, Thessaloniki, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Koulialis
authors.affiliation: Animal Health, Clinical Study Team Biologicals, P.O. Box 31, Boxmeer, 5830 AA, the Netherlands.
authors.forename: Tom
authors.initials: T
authors.lastname: Nell
authors.affiliation: Laboratory of Pathology, School of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, 54124, Thessaloniki, Greece.
authors.forename: Georgia
authors.initials: G
authors.lastname: Brellou
authors.affiliation: Farm Animals Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, St. Voutyra 11, 54627, Thessaloniki, Greece.
authors.forename: Eleni D
authors.initials: ED
authors.lastname: Tzika
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology; D005260:Female; D006115:Greece; D007112:Immunity, Maternally-Acquired; D008168:Lung / Q000473:pathology; D008297:Male; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000517:prevention & control*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D017778:Vaccines, Combined / Q000276:immunology; D014766:Viremia; D015430:Weight Gain
chemical_list: D017778:Vaccines, Combined
keywords: Enzootic pneumonia; Mycoplasma hyopneumoniae; Pigs; Porcilis PCV M Hyo; Porcine circovirus type 2