title: Phenotypic characterization of regulatory T cells from antiretroviral-naive HIV-1-infected people.
abstract: Regulatory T (Treg) cells play a key role in dampening excessive immune activation. However, antiretroviral therapy (ART) -naive HIV-1 infection maintains the immune system in a sustained state of activation that could alter both Treg cell surface markers and functions. As Treg cell surface markers are directly linked to their functions the overall objective of this study was to determine how ART-naive HIV infection affects the phenotypic properties of Treg cells. Our data showed that in ART-naive HIV-1 infection, Treg cells are dominated by effector (CD45RA+CD27-CCR7-CD62L-) and effector memory (CD45RA-CD27-CCR7-CD62L-) cells. In contrast Treg cells from HIV-negative individuals were mainly naive (CD45RA+CD27+CCR7+CD62L+) and central memory (CD45RA-CD27+CCR7+CD62L+) cells. Whereas effector and effector memory Treg cells showed enhanced expression of CD39 (P < 0·05), CD73 (P < 0·001), HLA-DR and CD38 (P < 0·001); naive and central memory Treg cells showed a significant reduction in the expression of these markers. Overall Treg cell frequencies within total CD4+T cells correlated positively with plasmatic HIV-1 viral load. As increased viral load is associated with augmented CD4+T-cell destruction; this could suggest a resistance of peripheral Treg cells to HIV-1 destruction. Hence the modulation of Treg cell phenotype and frequencies could be considered in designing immunotherapeutic strategies targeting immune system restoration during HIV-1 infection.
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.|Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde one, Yaounde, Cameroon.
authors.forename: Georgia N
authors.initials: GN
authors.lastname: Ambada
authors.affiliation: Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde one, Yaounde, Cameroon.
authors.forename: Claudine E
authors.initials: CE
authors.lastname: Ntsama
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.
authors.forename: Nadesh N
authors.initials: NN
authors.lastname: Nji
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.|Department of Biochemistry, Faculty of Sciences, University of Yaounde one, Yaounde, Cameroon.
authors.forename: Loveline N
authors.initials: LN
authors.lastname: Ngu
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.|Department of Biochemistry, Faculty of Sciences, University of Yaounde one, Yaounde, Cameroon.
authors.forename: Carole N
authors.initials: CN
authors.lastname: Sake
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.|Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde one, Yaounde, Cameroon.
authors.forename: Abel
authors.initials: A
authors.lastname: Lissom
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.|Department of Biochemistry, University of Dschang, Dschang, Cameroon.
authors.forename: Flaurent T
authors.initials: FT
authors.lastname: Tchouangeu
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.|Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde one, Yaounde, Cameroon.
authors.forename: Jules
authors.initials: J
authors.lastname: Tchadji
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.
authors.forename: Martin
authors.initials: M
authors.lastname: Sosso
authors.affiliation: Department of Microbiology, University of Yaounde one, Yaounde, Cameroon.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Etoa
authors.affiliation: Laboratory of Vaccinology/Biobanking, the Chantal Biya International Reference Centre for Research on the Prevention and Management of HIV/AIDS, Yaounde, Cameroon.
authors.forename: Godwin W
authors.identifier: 0000-0003-0611-0561
authors.initials: GW
authors.lastname: Nchinda
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use; D015703:Antigens, CD / Q000378:metabolism; D002163:Cameroon; D005260:Female; D015658:HIV Infections / Q000276:immunology* / Q000628:therapy; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D016219:Immunotherapy, Adoptive / Q000379:methods* / Q000639:trends; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000821:virology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000821:virology; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D015703:Antigens, CD
keywords: antiretroviral therapy-naive HIV infection; phenotype; regulatory T cells; sustained activation