title: Spatial landscape of oxygen in and around microvasculature during epileptic events.
abstract: Measuring changes in cerebral oxygen in tissue microdomains during epilepsy is important to identify hypoxic potential. Here, using a custom-built two-photon microscopy system, we present microscopic measurements of oxygen partial pressure ([Formula: see text]) in cortical microvessels and tissue of anesthetized mice during 4-aminopyridine (4-AP)-induced epileptic seizures. Investigating epileptic events, we characterized the distribution of the "initial dip" in [Formula: see text] in arterioles, venules, and tissue near the 4-AP injection site. Our results reveal a correlation between the percent change in [Formula: see text] during the "initial dip" and the diameter of nearest arterioles and venules.
authors.affiliation: École Polytechnique de Montréal, Department of Electrical Engineering, Montreal, Quebec, Canada; Montreal Heart Institute, Montreal, Quebec, Canada.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: École Polytechnique de Montréal, Department of Electrical Engineering, Montreal, Quebec, Canada; Montreal Heart Institute, Montreal, Quebec, Canada.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Moeini
authors.affiliation: École Polytechnique de Montréal, Department of Electrical Engineering, Montreal, Quebec, Canada; Montreal Heart Institute, Montreal, Quebec, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lesage
keywords: epilepsy; oxygen partial pressure; two-photon phosphorescence lifetime microscopy