title: Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl.
abstract: Dendrobium nobileLindl., an epiphytic herb distributed in the Southeast Asia, is used as a tonic and antipyretic herbal medicine in China. In this study, a water-soluble acidic heteropolysaccharide, DNP-W4, containing mannose, glucose, galactose, xylose, rhamnose, and galacturonic acid, in the molar ratios of 1.0:4.9:2.5:0.5:1.0:0.9, was obtained from the stems ofDendrobium nobileLindl. Using methylation analysis, partial acid hydrolysis, pectolyase treatment, NMR, and ESI-MS, the structure of DNP-W4 was elucidated. The obtained data indicated that DNP-W4 was a complex heteropolysaccharide and possessed a backbone composed of (1→4)-linked β-d-Glcp, (1→6)-linked β-d-Glcp, and (1→6)-linked β-d-Galp, with substitutes at O-4/6 of Glcp residues and O-3 of Galp. The branches of DNP-W4 were composed of terminal Manp, (1→6)-linked β-d-Manp, (1→3)-linked β-d-Glcp, β-d-Glcp, β-d-Galp, (1→4)-linked α-d-GalAp, (1→2)-linked α-L-Rhap, and Xylp. DNP-W4 had little immunological activities, but its derivatives had immuno-stimulating activities to some extent.
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. jianpingluo@hfut.edu.cn.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. zuoshurong727@163.com.
authors.forename: Shu-Rong
authors.initials: SR
authors.lastname: Zuo
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000818:Animals; D031667:Dendrobium / Q000737:chemistry*; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Dendrobium nobile Lindl.; acidic polysaccharide; immunological activities; polysaccharide