title: Antioxidant and anticancer effects of functional peptides from ovotransferrin hydrolysates.
abstract: BACKGROUND
  Egg white is a good source for making functional peptides that can be used in the food industry. Ovotransferrin (OTF) is one of the major egg white proteins, with many functional properties, including antioxidant, antimicrobial and antiviral activities. However, enzymatic hydrolysis of ovotransferrin is known to further improve the functional activities of OTF. The aim of this study was to investigate the antioxidant and anticancer activities of functional peptides produced by two-step enzyme hydrolysis of OTF.
  
  
  RESULTS
  OTF hydrolysates were prepared using promod 278P, thermolysin and a combination of the two enzymes. OTF and OTF hydrolysates showed strong antioxidant activities when analyzed using the DPPH assay. However, only OTF hydrolysates showed a strong free radical scavenging activity when NO- or ABTS-scavenging activity was measured. OTF hydrolysates showed stronger cytotoxic activities than the natural OTF against human cancer cell lines. Furthermore, OTF hydrolysates prepared with promod 278P + thermolysin combination showed the strongest cytotoxic activity, and their IC50values were 0.79, 0.78, 0.92 and 0.78 mg mL-1against AGS, LoVo, HT-29 and HeLa, respectively.
  
  
  CONCLUSIONS
  These results indicated that the two-step enzyme hydrolysates of OTF have great potential for use as a food ingredient with antioxidant and anticancer activities. © 2017 Society of Chemical Industry.
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, USA.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Korea.
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Kyungnam University, Changwon, Korea.
authors.forename: Eunju
authors.initials: E
authors.lastname: Park
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, USA.
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Ahn
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Korea.
authors.forename: Hyun-Dong
authors.identifier: http://orcid.org/0000-0001-9891-7703
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D003207:Conalbumin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010455:Peptides; D011492:Protein Hydrolysates; D003207:Conalbumin
keywords: anticancer; antioxidant; enzyme hydrolysis; ovotransferrin