title: Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata.
abstract: The ortholog of Aspergillus nidulans VelB, which is known as ClVelB, was studied to gain a broader insight into the functions of a velvet protein in Curvularia lunata. With the expected common and specific functions of ClVelB, the deletion of clvelB results in similar though not identical phenotypes. The pathogenicity assays revealed that ΔClVelB was impaired in colonizing the host tissue, which corresponds to the finding that ClVelB controls the production of conidia and the methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in C. lunata. However, the deletion of clvelB led to the increase in aerial hyphae and melanin formation. In addition, ΔClVelB showed a decreased sensitivity to iprodione and fludioxonil fungicides and a decreased resistance to cell wall-damaging agents and osmotic stress and tolerance to H2O2. The ultrastructural analysis indicated that the cell wall of ΔClVelB became thinner, which agrees with the finding that the accumulated level of glycerol in ΔClVelB is lower than the wild-type. Furthermore, the interaction of ClVelB with ClVeA and ClVosA was identified in the present research through the yeast two-hybrid and bimolecular fluorescence complementation assays. Results indicate that ClVelB plays a vital role in the regulation of various cellular processes in C. lunata.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Jin-Xin
authors.initials: JX
authors.lastname: Gao
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Chuan-Jin
authors.initials: CJ
authors.lastname: Yu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Jia-Nan
authors.initials: JN
authors.lastname: Sun
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Ya-Qian
authors.initials: YQ
authors.lastname: Li
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity* / Q000648:ultrastructure; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D008543:Melanins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009995:Osmosis; D018384:Oxidative Stress / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D011485:Protein Binding; D012100:Reproduction, Asexual / Q000187:drug effects; D064210:Secondary Metabolism / Q000187:drug effects; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D014118:Toxins, Biological / Q000096:biosynthesis; D014774:Virulence / Q000187:drug effects; D003313:Zea mays / Q000382:microbiology
chemical_list: D005656:Fungal Proteins; D005659:Fungicides, Industrial; D008543:Melanins; D014118:Toxins, Biological; D005990:Glycerol