title: Laser technique for the fabrication of blood vessels-like models for preclinical studies of pathologies under flow conditions.
abstract: In this work a method for fabricating functionalized preclinical devices is presented. The manufacturing process combines a laser indirect writing technique to fabricate a soda-lime glass master and soft-lithography methods to obtain the final structure in polydimethylsiloxane (PDMS). The roughness of the device is modified in a controlled manner by applying a post-thermal treatment to the master, and thus devices with different roughness values are created. The PDMS devices are fully covered with human umbilical vein cells in a two-step process. In order to determine the most suitable device to perform bioassays, the cell attachment to the channel is evaluated with regards to the walls roughness when flow experiments are carried out.
authors.affiliation: Photonics4Life Research Group, Departamento de Física Aplicada, Facultade de Física, Universidade de Santiago de Compostela E-15782, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Aymerich
authors.forename: E
authors.initials: E
authors.lastname: Álvarez
authors.forename: C
authors.initials: C
authors.lastname: Bao-Varela
authors.forename: I
authors.initials: I
authors.lastname: Moscoso
authors.forename: J R
authors.initials: JR
authors.lastname: González-Juanatey
authors.forename: M T
authors.initials: MT
authors.lastname: Flores-Arias
mesh_terms: D001672:Biocompatible Materials; D035843:Biomedical Research / Q000295:instrumentation / Q000379:methods; D017610:Calcium Compounds; D002478:Cells, Cultured; D004867:Equipment Design; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007834:Lasers; D008955:Models, Cardiovascular; D010087:Oxides; D012972:Sodium Hydroxide; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D010087:Oxides; C004569:soda lime; D012972:Sodium Hydroxide