title: Apoptotic Effects of Some Tetrahydronaphthalene Derivatives on K562 Human Chronic Myelogenous Leukemia Cell Line.
abstract: Retinoids which are vitamin A (Retinol) derivatives have been suggested to mediate the inhibition of cancer cell growth and apoptosis. It has been reported that all trans retinoic acid (ATRA) exhibited suppressive effects on different types of leukemia including chronic myelogenous leukemia.
  
  
  
  In the present study, we aim to find out the effects of 6 synthetic N-(3,5,5,8,8-pentamethyl-5,6,7,8- tetrahydronaphthalene-2-yl)-carboxamide derivatives (compound 6-12) on cell viability and apoptotic pathways in K562 human chronic myelogenous leukemia cell line.
  
  
  
  Cell viability and apoptosis were examined by spectrophotometric thiazolyl blue tetrazolium bromide (MTT) and caspase-3 assay, western blot, RT-PCR and flow cytometry.
  
  
  
  Our results indicated that compound 6 (5-(1,2-Dithiolan-3-yl)-N-(3,5,5,8,8-pentamethyl-5,6,7,8- tetrahydronaphthalen-2-yl)pentanamide), 8 (4-(3,4-Dimethoxyphenyl)-N-(3,5,5,8,8-pentamethyl-5,6,7,8- tetrahydronaphthalen-2-yl)butanamide) and 11 (E-3-(4-Hydroxy-3-methoxyphenyl)-N-(3,5,5,8,8-pentamethyl- 5,6,7,8-tetrahydronaphthalen-2-yl)acrylamide) exhibited apoptotic effects in K562 human chronic myelogenous leukemia cell line and induced caspase 3, PARP cleavage, Bax/Bcl-2 ratio, Bad and Bim gene expressions.
  
  
  
  Some retinoid derivatives tested in this study induced apoptosis of K562 cells which suggest that these compounds may serve as potential agents in the treatment of chronic myelogenous leukemia.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Koc
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Tulin
authors.initials: T
authors.lastname: Ozkan
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Yalda
authors.initials: Y
authors.lastname: Hekmatshoar
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: A Selen
authors.initials: AS
authors.lastname: Gurkan-Alp
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: Fugen
authors.initials: F
authors.lastname: Aktan
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Buyukbingol
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Asuman
authors.initials: A
authors.lastname: Sunguroglu
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: Erdem
authors.initials: E
authors.lastname: Buyukbingol
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
authors.forename: Arzu Zeynep
authors.initials: AZ
authors.lastname: Karabay
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D013764:Tetrahydronaphthalenes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D013764:Tetrahydronaphthalenes
keywords: K562; Retinoids; apoptosis; cancer; chronic myelogenous leukemia; tetrahydronaphthalene.