title: Protective effect ofBifidobacterium infantisCGMCC313-2 on ovalbumin-induced airway asthma and β-lactoglobulin-induced intestinal food allergy mouse models.
abstract: OBJECTIVE
  To determine whether oral administration ofBifidobacterium infantisCGMCC313-2 (B. infantisCGMCC313-2) inhibits allergen-induced airway inflammation and food allergies in a mouse model.
  
  
  METHODS
  Ovalbumin (OVA)-induced allergic asthma and β-lactoglobulin-induced food allergy mouse models were used in this study. Following oral administration ofB. infantisCGMCC313-2 during or after allergen sensitization, histopathologic changes in the lung and intestine were evaluated by hematoxylin and eosin (HE) staining. In the allergic asthma mouse model, we evaluated the proportion of lung-infiltrating inflammatory cells. OVA-specific IgE and IgG1 levels in serum and cytokine levels in bronchoalveolar lavage fluid (BALF) were also assessed. In the food allergy mouse model, the levels of total IgE and cytokines in serum were measured.
  
  
  RESULTS
  Oral administration ofB. infantisCGMCC313-2 during or after allergen sensitization suppressed allergic inflammation in lung and intestinal tissues, while the proportion of infiltrating inflammatory cells was significantly decreased in the BALF of allergic asthma mice. Moreover,B. infantisCGMCC313-2 decreased the serum levels of total IgE in food allergy mice, and reductions in IgE and IgG1 were also observed in OVA-induced allergic asthma mice. The expression of interleukin-4 (IL-4) and IL-13 in both serum and BALF was suppressed following the administration ofB. infantisCGMCC313-2, while an effect on serum IL-10 levels was not observed.
  
  
  CONCLUSIONS
  B. infantisCGMCC313-2 inhibits the secretion of allergen-induced IgE, IL-4 and IL-13, and attenuates allergic inflammation.
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Meng-Yun
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Yang
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Wen-Kui
authors.initials: WK
authors.lastname: Dai
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Jian-Qiong
authors.initials: JQ
authors.lastname: Huang
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Yin-Hu
authors.initials: YH
authors.lastname: Li
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Chuang-Zhao
authors.initials: CZ
authors.lastname: Qiu
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wei
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Dong-Fang
authors.initials: DF
authors.lastname: Li
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Wang
authors.affiliation: Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children's Hospital, Shenzhen 518026, China.
authors.forename: Yue-Jie
authors.initials: YJ
authors.lastname: Zheng
mesh_terms: D000485:Allergens / Q000737:chemistry; D000818:Animals; D001249:Asthma / Q000139:chemically induced*; D000070236:Bifidobacterium longum subspecies infantis; D001992:Bronchoalveolar Lavage Fluid; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000517:prevention & control* / Q000628:therapy; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007249:Inflammation; D018793:Interleukin-13 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007422:Intestines / Q000276:immunology*; D007782:Lactoglobulins / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000737:chemistry; D019936:Probiotics
chemical_list: D000485:Allergens; D016207:Cytokines; D007074:Immunoglobulin G; D018793:Interleukin-13; D007782:Lactoglobulins; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: Allergy; Asthma; Bifidobacterium infantis; Ovalbumin; β-lactoglobulin