title: Protein/Protein, DNA/DNA and DNA/Protein based vaccination strategies using truncated Omp2b against Brucella infection in BALB/c Mice.
abstract: The purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (TOmp2b) from Brucella abortus in BALB/c mice. Three immunization regimens Protein/Protein, DNA/DNA and DNA/Protein were used. Immunization of mice with all vaccine strategies elicited a strong specific IgG responses (IgG2a titers over IgG1) and provided T helper1 (Th1) oriented immune responses. Furthermore, Protein/Protein (Pro/Pro-) and DNA/Pro- vaccinated groups conferred protection levels against B. abortus challenge not significantly different from those induced by B. abortus RB51 vaccine stain. In conclusion, TOmp2b is potential to stimulate specific immune responses and to confer cross protection against B. melitensis and B. abortus infection. Therefore, TOmp2b could be introduced as a new subunit vaccine candidate against Brucella infection.
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Golshani
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Rafati
authors.affiliation: Bacterial Vaccine and Antigen Production Branch, Pasteur Institute of Iran, Karaj, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nejati-Moheimani
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran; Department of Biology, Azad University of North branch, Tehran, Iran.
authors.forename: Somaye
authors.initials: S
authors.lastname: Pourabdi
authors.affiliation: Bacterial Vaccine and Antigen Production Branch, Pasteur Institute of Iran, Karaj, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Arsang
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: saeidbouzari@yahoo.com.
authors.forename: Saeid
authors.initials: S
authors.lastname: Bouzari
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control*; D004269:DNA, Bacterial / Q000276:immunology*; D005260:Female; D000071497:Immunogenicity, Vaccine; D051379:Mice; D008807:Mice, Inbred BALB C; D018272:Porins / Q000235:genetics / Q000276:immunology*; D011487:Protein Conformation; D015203:Reproducibility of Results; D018417:Th1 Cells / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002004:Brucella Vaccine; D004269:DNA, Bacterial; C096736:Omp2b protein, Brucella; D018272:Porins; D019444:Vaccines, DNA; D022223:Vaccines, Subunit
keywords: Brucella abortus; DNA vaccine; Prime-boost strategy; Recombinant protein; TOmp2b Brucella melitensis