title: Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects.
abstract: OBJECTIVE
  The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot.
  
  
  METHODS
  Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media. Substitutes were immunocharacterized with collagen IV and cytokeratin 5-14 as specific markers. A Student´s t- test was performed to assess the differences between both groups.
  
  
  RESULTS
  It was possible to isolate epidermal cells from the oral mucosa of diabetic and healthy subjects and develop autologous dermal-epidermal skin substitutes using autologous serum as a supplement. Differences in the expression of specific markers were observed and the cytokeratin 5-14 expression was lower in the diabetic substitutes, and the collagen IV expression was higher in the diabetic substitutes when compared with the healthy group, showing a significant difference.
  
  
  CONCLUSIONS
  Cells from oral mucosa could be an alternative and less invasive source for skin substitutes and wound healing. A difference in collagen production of diabetic cells suggests diabetic substitutes could improve diabetic wound healing. More research is needed to determine the crosstalk between components of these skin substitutes and damaged tissues.
authors.affiliation: Universidad Autónoma de San Luis Potosí, Facultad de Estomatología, Grupo de Investigación en Ingeniería Tisular, San Luis Potosí, México.|Universidad Autónoma de San Luis Potosí, Facultad de Medicina, Maestría en Ciencias en Investigación Clínica, San Luis Potosí, México.
authors.forename: Daniela
authors.initials: D
authors.lastname: Guzmán-Uribe
authors.affiliation: Universidad Autónoma de San Luis Potosí, Facultad de Estomatología, Grupo de Investigación en Ingeniería Tisular, San Luis Potosí, México.
authors.forename: Keila Neri
authors.initials: KN
authors.lastname: Alvarado-Estrada
authors.affiliation: Universidad Autónoma de San Luis Potosí, Facultad de Medicina, Maestría en Ciencias en Investigación Clínica, San Luis Potosí, México.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Pierdant-Pérez
authors.affiliation: Hospital Central Dr Ignacio Morones Prieto, Departamento de Dermatología, San Luis Potosí, México.
authors.forename: Bertha
authors.initials: B
authors.lastname: Torres-Álvarez
authors.affiliation: Universidad Autónoma de San Luis Potosí, Facultad de Medicina, Maestría en Ciencias en Investigación Clínica, San Luis Potosí, México.
authors.forename: Jesus Martin
authors.initials: JM
authors.lastname: Sánchez-Aguilar
authors.affiliation: Universidad Autónoma de San Luis Potosí, Facultad de Estomatología, Grupo de Investigación en Ingeniería Tisular, San Luis Potosí, México.|Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Iztacala, Laboratorio en Ingeniería Tisular y Medicina Traslacional, Tlanepantla, México.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rosales-Ibáñez
mesh_terms: D000328:Adult; D000368:Aged; D001672:Biocompatible Materials; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D003924:Diabetes Mellitus, Type 2 / Q000628:therapy; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000637:transplantation*; D005260:Female; D005347:Fibroblasts; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology*; D015203:Reproducibility of Results; D012883:Skin Ulcer / Q000628:therapy; D017768:Skin, Artificial; D013997:Time Factors; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D003094:Collagen