title: In vivo human corpus cavernosum regeneration: fabrication of tissue-engineered corpus cavernosum in rat using the body as a natural bioreactor.
abstract: OBJECTIVE
  Few researches have been conducted to develop an ideal method for tissue engineering of corpus cavernosum. We produce a decellularized human corpus cavernosum scaffold and investigate the in vivo cell seeding of the scaffold after transplantation into the rat omentum.
  
  
  METHODS
  Eight adult human male corpus cavernosum were obtained in sterile condition. After dissecting the urethra, corpus cavernosum was decellularized by inserting an 18-gauge needle into the body of the tissue. The gauge was connected to a peristaltic pump to circulate the detergents in the corpus. Several assessments were performed to evaluate the efficacy of decellularization and extracellular matrix (ECM) preservation. A section of decellularized scaffold was washed several times and transplanted into the omentum of 4 male healthy Sprague-Dawley rats and located into the scrotum. Biopsies were taken 1, 3, and 6 months after transplantation. Histological examination, SEM, and immunohistochemical staining were performed to assess the efficacy of natural recellularization.
  
  
  RESULTS
  The results of the examinations performed prior to transplantation revealed a decellularized ECM resembling to the native tissue with normal pits that may be appropriate for further in vivo cell seeding. Histopathology examination of the biopsies after transplantations confirmed successful cell seeding with endothelium-like cells in different time points. CD34 staining was dominant in the short-time biopsies, while CD31 staining was higher than CD34 in long-term specimens.
  
  
  CONCLUSIONS
  The feasibility of natural bioreactor in recellularizing corpus cavernosum was confirmed. This technique may have the potential to facilitate homologous transplantation for repair of corpus defects.
authors.affiliation: Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419433151, Iran. kajbafzd@sina.tums.ac.ir.
authors.forename: Abdol-Mohammad
authors.initials: AM
authors.lastname: Kajbafzadeh
authors.affiliation: Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419433151, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Abbasioun
authors.affiliation: Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419433151, Iran.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Sabetkish
authors.affiliation: Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419433151, Iran.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Sabetkish
authors.affiliation: Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419433151, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Habibi
authors.affiliation: Department of Urology and Renal Transplantation, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Kamyar
authors.initials: K
authors.lastname: Tavakkolitabassi
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D005109:Extracellular Matrix / Q000637:transplantation*; D006801:Humans; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D009852:Omentum; D010413:Penis / Q000166:cytology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D000093667:Penile Transplantation
chemical_list: D018952:Antigens, CD34; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Extracellular matrix; Scaffold; Tissue engineering; Transplantation