title: Micro RNA-19a suppresses interleukin-10 in peripheral B cells of patients with diabetic retinopathy.
abstract: A number of patients with diabetes suffer from retinopathy; the pathogenesis is to be further investigated. Recent reports indicate that micro RNA (miR) plays critical roles in the development of immune inflammation. This study test a hypothesis that miR-17-92 cluster is associated with the pathogenesis of diabetes retinopathy (DR). In this study, peripheral blood samples were collected from DR patients and healthy subjects. B cells were isolated from the blood samples to be analyzed the expression of interleukin (IL)-10. The results showed that lower levels of IL-10 were detected in peripheral B cells of DR patients as compared with healthy subjects. miR-19a was increased in B cells of DR patients, which was negatively correlated with the IL-10 expression. Exposure of naive B cells to IL-17 increased the expression of miR-19a and suppression of IL-10 expression in the B cells, in which histone deacetylase 11 (HDAC 11) played a critical role. In conclusion, the IL-17 suppresses IL-10 expression in peripheral B cells via enhancing miR-19a expression and HDAC activity in DR patients. The miR-19a and HDAC 11 may be novel therapeutic targets in the treatment of DR.
authors.affiliation: Beijing Tongren Eye Centre, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmol and Vis Sci Key Lab Beijing 100730, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Lab Beijing 100730, China.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: You
authors.affiliation: Beijing Tongren Eye Centre, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmol and Vis Sci Key Lab Beijing 100730, China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Ophthalmology, 306th Hospital of PLA Beijing 100101, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, Beijing Anzhen Hospital, Capital Medical University Beijing 100029, China.
authors.forename: Xinxiao
authors.initials: X
authors.lastname: Gao
authors.affiliation: Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Lab Beijing 100730, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Peng
keywords: Autoimmunity; B lymphocyte; interleukin 10; interleukin 17; retinopathy