title: Comparative Bone Regeneration Potential Studies of Collagen, Heparin, and Polydopamine-Coated Multichannelled BCP Granules.
abstract: The current study is a comparative assessment of the bone regeneration potentiality of bone substitutes composed of bioactive polymer-coated biphasic calcium phosphate (BCP) granules. The bone substitutes were primarily composed of multichanneled BCP granules, which were coated separately with biochemical polymer coatings, namely collagen, heparin, or polydopamine (PD), using chemical methods. The morphologic features and chemical structure of the granules and surface coatings were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. The biological characterizations in terms of cellular interaction with the bone substitutes in vitro (MTT assay and proliferation) and in vivo (bone regeneration efficacy) were performed and compared with uncoated BCP granules. It was found that PD-coated BCP granules were superior to the others with respect to promoting more rapid healing. Therefore, PD multichannel BCP granule system can be used as a promising injectable bone substitute for clinical applications.
authors.affiliation: From the Department of Regenerative Medicine, College of Medicine and Institute of Tissue Regeneration, Soonchunhyang University, Cheonan, South Korea.
authors.forename: Gun Hee
authors.initials: GH
authors.lastname: Lee
authors.affiliation: From the Department of Regenerative Medicine, College of Medicine and Institute of Tissue Regeneration, Soonchunhyang University, Cheonan, South Korea.
authors.forename: Preeti
authors.initials: P
authors.lastname: Makkar
authors.affiliation: From the Department of Regenerative Medicine, College of Medicine and Institute of Tissue Regeneration, Soonchunhyang University, Cheonan, South Korea.
authors.forename: Kallyanshis
authors.initials: K
authors.lastname: Paul
authors.forename: Byong Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology; D001842:Bone and Bones / Q000503:physiopathology; D018929:Cell Culture Techniques; D003094:Collagen / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects; D011108:Polymers / Q000494:pharmacology*; D011817:Rabbits; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; D007211:Indoles; D011108:Polymers; C074950:hydroxyapatite-beta tricalcium phosphate; C568283:polydopamine; D006493:Heparin; D003094:Collagen