title: Facile and efficient electrochemical enantiomer recognition of phenylalanine using β-Cyclodextrin immobilized on reduced graphene oxide.
abstract: This work demonstrates the facile and efficient preparation protocol of β-Cyclodextrin-reduced graphene oxide modified glassy carbon electrode (β-CD/RGO/GCE) sensor for an impressive chiral selectivity analysis for phenylalanine enantiomers. In this work, the immobilization of β-CD over graphene sheets allows the excellent enantiomer recognition due to the large surface area and high conductivity of graphene sheets and extraordinary supramolecular (host-guest interaction) property of β-CD. The proposed sensor was well characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and electrochemical impedance spectroscopy (EIS) techniques. The analytical studies demonstrated that the β-CD/RGO/GCE exhibit superior chiral recognition toward L-phenylalanine as compared to D-phenylalanine. Under optimum conditions, the developed sensor displayed a good linear range from 0.4 to 40µM with the limit of detection (LOD) values of 0.10µM and 0.15µM for l- and D-phenylalanine, respectively. Furthermore, the proposed sensor exhibits good stability and regeneration capacity. Thus, the as-synthesized material can be exploited for electrochemical enantiomer recognition successfully.
authors.affiliation: Department of Chemistry, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea. Electronic address: shabizaidi79@gmail.com.
authors.forename: Shabi Abbas
authors.initials: SA
authors.lastname: Zaidi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010087:Oxides; D047392:beta-Cyclodextrins; D010649:Phenylalanine; D002244:Carbon; D006108:Graphite
keywords: Enantiomer recognition; Graphene; Phenylalanine; β-Cyclodextrin