title: rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats.
abstract: The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.|Faculdade de Medicina Veterinária, Pontifícia Universidade Católica do Paraná - PUCPR, Toledo, PR, Brasil.
authors.forename: Dauton Luiz
authors.initials: DL
authors.lastname: Zulpo
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso - UFMT, Cuiabá, MT, Brasil.
authors.forename: Michelle
authors.initials: M
authors.lastname: Igarashi
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Ana Sue
authors.initials: AS
authors.lastname: Sammi
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Joeleni Rosa Dos
authors.initials: JR
authors.lastname: Santos
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: João Pedro
authors.initials: JP
authors.lastname: Sasse
authors.affiliation: Faculdade de Medicina Veterinária, Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão - UFMA, São Luís, MA, Brasil.
authors.forename: Ivo Alexandre Leme da
authors.initials: IA
authors.lastname: Cunha
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Taroda
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Luiz Daniel de
authors.initials: LD
authors.lastname: Barros
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Jonatas Campos de
authors.initials: JC
authors.lastname: Almeida
authors.affiliation: Animal Parasitic Disease Laboratory, United States Department of Agriculture, Annapolis, MD, USA.
authors.forename: Mark Christopher
authors.initials: MC
authors.lastname: Jenkins
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Italmar Teodorico
authors.initials: IT
authors.lastname: Navarro
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D000913:Antibodies, Protozoan; D002371:Cat Diseases / Q000276:immunology / Q000517:prevention & control*; D002415:Cats; D008565:Membrane Proteins / Q000008:administration & dosage / Q000276:immunology*; D033661:Oocysts / Q000276:immunology; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D062605:Quillaja Saponins / Q000008:administration & dosage / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D062605:Quillaja Saponins; C090555:ROP 2 protein, Toxoplasma gondii; D011994:Recombinant Proteins; C046386:Quil A