title: Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model.
abstract: The use of the existing endogenous neural progenitor cells (NPCs) in the brains of adult mammalian animals is challenging for cell therapy in treating Parkinson's disease (PD). Previous studies have indicated that there is a low level of neurogenesis in the substantia nigra (SN) of adult mice. To assess the regenerative/neurogenic capacity of NPCs following an intranigral injection of 6-hydroxydopamine (6-OHDA), the proliferation and differentiation of subventricular zone (SVZ)- and midbrain-derived NPCs were investigated, and the origin of SN newborn dopaminergic neurons was traced by using Nestin-CreERTM::ROSA26-LacZ mice and constructing a plasmid CD133-Promoter2-Cre. Our results showed that an intranigral injection of 6-OHDA-induced loss of dopaminergic neurons produced a significant increase in the SVZ-derived NPCs of the third ventricle (3V), cerebral aqueduct (Aq), and their surrounding regions. The SN newly generated dopaminergic neurons might contribute a little to an incomplete recovery of the nigrostriatal system. In addition, we found that SN newborn dopaminergic neurons were mainly derived from the migration and differentiation of the NPCs in the 3V- and Aq-SVZ and their adjacent regions. Thus, it will become an ideal strategy to treat PD by promoting the proliferation and differentiation of endogenous NPCs.
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Xie
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Taizhou Affiliated Hospital of Wenzhou Medical University, Taizhou, Zhejiang Province, 317000, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Department of Histology and Embryology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: T T
authors.initials: TT
authors.lastname: Wang
authors.affiliation: Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Ding
authors.affiliation: Department of Pharmacy, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, China.
authors.forename: S S
authors.initials: SS
authors.lastname: Qi
authors.affiliation: Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: S X
authors.initials: SX
authors.lastname: Chen
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Shao
authors.affiliation: Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Department of Histology and Embryology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China.
authors.forename: M
authors.initials: M
authors.lastname: Liao
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China; Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, China. Electronic address: sunchenyou1972@aliyun.com.
authors.forename: C Y
authors.initials: CY
authors.lastname: Sun
mesh_terms: D000071916:AC133 Antigen / Q000235:genetics / Q000378:metabolism; D018663:Adrenergic Agents / Q000633:toxicity*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D003342:Corpus Striatum / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects*; D004965:Estrogen Antagonists / Q000494:pharmacology; D020547:Lateral Ventricles / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects; D016627:Oxidopamine / Q000633:toxicity*; D010300:Parkinson Disease / Q000209:etiology / Q000473:pathology*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D000071916:AC133 Antigen; D018663:Adrenergic Agents; D004965:Estrogen Antagonists; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D064231:Nestin; D022661:RNA, Untranslated; D013629:Tamoxifen; D016627:Oxidopamine
keywords: 6-hydroxydopamine; Parkinson’s disease; dopaminergic neuron; neural progenitor cell; neurogenesis; substantia nigra