title: Hydrogen Peroxide-Induced Secreted Frizzled-Related Protein 1 Gene Demethylation Contributes to Hydrogen Peroxide-Induced Apoptosis in Human U251 Glioma Cells.
abstract: Glioblastoma multiforme is a type of central nervous system tumor with extremely poor prognosis. Previously, hydrogen peroxide (H2O2), which promotes the oxidative stress response, has been reported to induce the apoptosis of glioma cells. Recently, secreted frizzled-related protein 1 (SFRP1) has been shown to be associated with various types of malignant tumors and with H2O2-induced oxidative stress in cardiomyocytes by negatively regulating the Wnt signaling pathway. This study aimed to explore SFRP1 expression and its roles in H2O2-induced apoptosis in human glioma cells. We found that the SFRP1 level was decreased in several human glioma cell lines, including U87, U251, and SW1783 cells. In U251 cells, SFRP1 could function as a cancer suppressor gene, and the growth of U251 cells could be inhibited not only by H2O2but also by the overexpression of SFRP1. Furthermore, we demonstrated that H2O2-induced SFRP1 gene demethylation partially contributed to H2O2-induced U251 cell apoptosis, which was verified by studies using an SFRP inhibitor (WAY-316606). Our research identified that H2O2-induced SFRP1 gene demethylation contributes to H2O2-induced apoptosis in human U251 glioma cells.
authors.affiliation: 1 Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical University , Xi'an, China .
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Xing
authors.affiliation: 2 Department of General Medicine and Geriatrics, The First Affiliated Hospital of Xi'an Medical University , Xi'an, China .
authors.forename: Yaping
authors.initials: Y
authors.lastname: Ni
authors.affiliation: 3 Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an, China .
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: 3 Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an, China .
authors.forename: Xudong
authors.initials: X
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D019175:DNA Methylation / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D006861:Hydrogen Peroxide; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C117636:SFRP1 protein, human; D000094742:Secreted Frizzled-Related Proteins
keywords: U251 cells; cell apoptosis; demethylation; hydrogen peroxide; secreted frizzled-related protein 1