title: Investigation of 8-OHdG, CYP1A, HSP70 and transcriptional analyses of antioxidant defence system in liver tissues of rainbow trout exposed to eprinomectin.
abstract: Eprinomectin (EPM), a member of avermectin family, is a semi-synthetic antibiotic. It has been known that avermectin family enters the aquatic environments and adversely affects the aquatic organisms. Effects of EPM is fully unknown in aquatic organisms especially fish, thus the aim of the present study was to investigate transcriptional changes (sod, cat, gpx) and activities of some antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)) and transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A) in liver tissues of rainbow trout exposed to sublethal EPM concentration (0.001 μg/L, 0.002 μg/L, 0.01 μg/L, 0.05 μg/L) for 24 h, 48 h, 72 h and 96 h. The decrease in antioxidant enzyme (SOD, CAT and GPx) activity, transcriptional changes (sod, cat, gpx, HSP70 and CYP1A genes) and increase in MDA level and activity of 8-OHdG in a dose-time-dependent manner in the liver of rainbow trout were observed. The down-regulated of antioxidant (sod, cat and gpx), HSP70 and CYP1A obviously, the severity of which increased with the concentration of EPM and exposure time. The results imply that EPM could induce oxidative damage to the liver tissue of rainbow trout. The information presented in this study is helpful to understand the mechanism of veterinary pharmaceuticals-induced oxidative stress in fishes.
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey. Electronic address: galak@atauni.edu.tr.
authors.forename: Gonca
authors.initials: G
authors.lastname: Alak
authors.affiliation: Department of Chemistry, Faculty of Science, University of Yuzuncu Yıl, TR-65080, Van, Turkey.
authors.forename: Aslı Çilingir
authors.initials: AÇ
authors.lastname: Yeltekin
authors.affiliation: Department of Parasitology, Faculty of Veterinary, Ataturk University, TR-25030 Erzurum, Turkey.
authors.forename: Ismail Hakkı
authors.initials: IH
authors.lastname: Tas
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Ucar
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey.
authors.forename: Veysel
authors.initials: V
authors.lastname: Parlak
authors.affiliation: Department of Basic Sciences, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Topal
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey.
authors.forename: Esat Mahmut
authors.initials: EM
authors.lastname: Kocaman
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Atamanalp
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D000072461:Cytochrome P450 Family 1 / Q000235:genetics / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D007306:Insecticides; D007559:Ivermectin; C101434:eprinomectin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000072461:Cytochrome P450 Family 1; D003849:Deoxyguanosine
keywords: 8-OHdG; Antibiotic; CYP1A; Eprinomectin; Fish; HSP70; Liver; Oxidative stress