title: Parental diuron-exposure alters offspring transcriptome and fitness in Pacific oyster Crassostrea gigas.
abstract: One of the primary challenges in ecotoxicology is to contribute to the assessment of the ecological status of ecosystems. In this study, we used Pacific oyster Crassostrea gigas to explore the effects of a parental exposure to diuron, a herbicide frequently detected in marine coastal environments. The present toxicogenomic study provides evidence that exposure of oyster genitors to diuron during gametogenesis results in changes in offspring, namely, transcriptomic profile alterations, increased global DNA methylation levels and reduced growth and survival within the first year of life. Importantly, we highlighted the limitations to identify particular genes or gene expression signatures that could serve as biomarkers for parental herbicide-exposure and further for multigenerational and transgenerational effects of specific chemical stressors. By analyzing samples from two independent experiments, we demonstrated that, due to complex confounding effects with both tested solvent vehicles, diuron non-specifically affected the offspring transcriptome. These original results question the potential development of predictive genomic tools for detecting specific indirect impacts of contaminants in environmental risk assessments. However, our results indicate that chronic environmental exposure to diuron over several generations may have significant long term impacts on oyster populations with adverse health outcomes.
authors.affiliation: Ifremer, UMR 5244, IHPE Interactions-Hosts-Pathogens-Environments, UPVD, CNRS, Université de Montpellier, CC 80, F-34095 Montpellier, France. Electronic address: ebachere@ifremer.fr.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Bachère
authors.affiliation: Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes cedex 03, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Barranger
authors.affiliation: Acobiom, 1682 rue de la Valsière, CS 77394 Cap Delta Biopole Euromédecine II, 34184 Montpellier Cedex 04, France.
authors.forename: Roman
authors.initials: R
authors.lastname: Bruno
authors.affiliation: Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes cedex 03, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Rouxel
authors.affiliation: Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes cedex 03, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Menard
authors.affiliation: Acobiom, 1682 rue de la Valsière, CS 77394 Cap Delta Biopole Euromédecine II, 34184 Montpellier Cedex 04, France; Diag4Zoo, 1 rue des Loutres, 34170 Montpellier, France.
authors.forename: David
authors.initials: D
authors.lastname: Piquemal
authors.affiliation: Ifremer, Laboratoire d'Ecotoxicologie, Rue de l'Ile d'Yeu, BP21105, 44311 Nantes cedex 03, France.
authors.forename: Farida
authors.initials: F
authors.lastname: Akcha
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000187:drug effects* / Q000254:growth & development; D019175:DNA Methylation / Q000187:drug effects; D004237:Diuron / Q000633:toxicity*; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis; D005718:Gametogenesis / Q000187:drug effects* / Q000235:genetics; D055106:Genome-Wide Association Study; D006540:Herbicides / Q000633:toxicity*; D043922:Toxicogenetics; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D014874:Water Pollutants, Chemical; D004237:Diuron
keywords: Biomarkers; Global DNA-methylation; Herbicide; Marine bivalve; Survival; Toxicogenomics