title: Polydatin impairs mitochondria fitness and ameliorates podocyte injury by suppressing Drp1 expression.
abstract: Polydatin (PD), a resveratrol glycoside, has been shown to protect renal function in diabetic nephropathy (DN), but the underlying molecular mechanism remains unclear. This study demonstrates that PD stabilize the mitochondrial morphology and attenuate mitochondrial malfunction in both KKAy mice and in hyperglycemia (HG)-induced MPC5 cells. We use Western blot analysis to demonstrate that PD reversed podocyte apoptosis induced by HG via suppressing dynamin-related protein 1 (Drp1). This effect may depend on the ability of PD to inhibit the generation of cellular reactive oxygen species (ROS). In conclusion, we demonstrate that PD may be therapeutically useful in DN, and that, podocyte apoptosis induced by HG can be reversed by PD through suppressing Drp1 expression.
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Zheng
authors.identifier: http://orcid.org/0000-0001-7314-4249
authors.initials: Z
authors.lastname: Ni
authors.affiliation: College of Stomatology of Guangxi Medical University, Nanning, Guangxi, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tao
authors.affiliation: Pharmaceutical College, Guangxi Medical University, Nanning, Guangxi, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Xiaohui
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zelin
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Jiangang
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Huo
authors.initials: H
authors.lastname: Weikang
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hongchao
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangdong Province, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Qiujing
authors.affiliation: Shenzhen Hospital of Southern Medical University, Shenzhen, Guangdong, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001786:Blood Glucose / Q000378:metabolism; D002460:Cell Line; D019610:Cytoprotection; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D034281:Dynamins / Q000235:genetics / Q000378:metabolism*; D005960:Glucosides / Q000494:pharmacology*; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D063154:Mitochondrial Dynamics / Q000187:drug effects; D050199:Podocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D014162:Transfection
chemical_list: D001786:Blood Glucose; D005960:Glucosides; D017382:Reactive Oxygen Species; D013267:Stilbenes; C486391:Dnm1l protein, mouse; D034281:Dynamins; C058229:polydatin
keywords: diabetic nephropathy; dynamin-related protein 1; polydatin