title: Regulation of protein-binding activities of molecularly imprinted polymers via post-imprinting modifications to exchange functional groups within the imprinted cavity.
abstract: We prepared lysozyme-imprinted polymers bearing modifiable sites within the imprinted cavity to introduce various functional groups via post-imprinting modifications. For this purpose, ({[2-(2-methacrylamido)-ethyldithio]-ethylcarbamoyl}-methoxy)acetic acid (MDTA), which has a carboxy group to interact with the target protein, lysozyme, and a disulfide linkage for post-imprinting modifications, was used as a functional monomer. A lysozyme-imprinted polymer film was prepared by copolymerization of MDTA with a cross-linker, N,N'-methylenebisacrylamide, in the presence of lysozyme. After removing lysozyme, followed by reducing the disulfide linkage, various functional groups, such as carboxy, amino, sulfonate, and oligo-ethylene oxide, were introduced to the exposed thiol groups via a disulfide exchange reaction using the pyridyldisulfide derivatives of these functional groups. Various functional groups could be introduced reversibly via this post-imprinting disulfide exchange reaction after the construction of the lysozyme-imprinted cavities. The modification regulated the protein-binding activity. The proposed post-imprinting modification system, based on a molecular imprinting process, is expected to lead to the development of advanced materials for fine-tuning and/or introducing desired functions.
authors.affiliation: Graduate School of Engineering, Kobe University, Kobe, Japan.
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Sunayama
authors.affiliation: Graduate School of Engineering, Kobe University, Kobe, Japan.
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Kitayama
authors.affiliation: Graduate School of Engineering, Kobe University, Kobe, Japan.
authors.forename: Toshifumi
authors.identifier: 0000-0002-5641-2333
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D054802:Molecular Imprinting; D009113:Muramidase / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000178:Acrylamides; D004220:Disulfides; D011108:Polymers; D011506:Proteins; D009113:Muramidase; C021221:N,N'-methylenebisacrylamide
keywords: molecular imprinting; polymers; post-imprinting modification; sensor; synthetic receptor