title: De novo analysis of the oriental armyworm Mythimna separata antennal transcriptome and expression patterns of odorant-binding proteins.
abstract: To better understand the olfactory mechanisms in the oriental armyworm Mythimna separate, one of the most serious pests of cereals, an antennal transcriptome was constructed in this study. A total of 130 olfactory related transcripts were identified. These transcripts were predicted to encode 32 odorant-binding proteins (OBPs), 16 chemosensory proteins (CSPs), 71 olfactory receptors (ORs), 8 ionotropic receptors (IRs), 1 gustatory receptor (GR) and 2 sensory neuron membrane proteins (SNMPs). Q-PCR analysis of the temporal expression profiles of seven OBPs in different tissues indicated that, except for MsepOBP19 which was highly expressed in the wings of 0-day-old adult and MsepOBP20 which was low expressed in all tissues, other tested MsepOBPs were significantly more highly expressed in the antenna than in the head (antenna excluded), thorax, abdomen, legs and wings. The expression levels of MsepOBPs were diverse in different life stages (differed on eclosion days). MsepOBP5 exhibited female-biased expression in 0- and 5-day-old adult, while no gender bias in 1- and 3-day-old adult was detected and similar expression profiles were found for MsepOBP7, 20, 24 and 26. In addition, we found that although the expression of MsepOBP22 was female biased in 0- and 5-day-old adult, in the 3-day-old adult it was male-biased. Our findings established a foundation for future studies of the functions of olfactory proteins in M. separata.
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hubei Province Key Laboratory for Crop Diseases, Insect Pests and Weeds Control, Institute of Plant Protection & Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430068, People's Republic of China.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Chang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Xiao-Pei
authors.initials: XP
authors.lastname: Nie
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Zeng
authors.affiliation: Hubei Province Key Laboratory for Crop Diseases, Insect Pests and Weeds Control, Institute of Plant Protection & Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430068, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Lv
authors.affiliation: Hubei Province Key Laboratory for Crop Diseases, Insect Pests and Weeds Control, Institute of Plant Protection & Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430068, People's Republic of China. Electronic address: ricezs6410@163.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: mqwang@mail.hzau.edu.cn.
authors.forename: Man-Qun
authors.initials: MQ
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000254:growth & development / Q000378:metabolism*; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D009036:Moths / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D018035:Receptors, Odorant / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Antennal transcriptome; Chemosensory proteins; Expression patterns; Mythimna separata