title: Identification of short single disulfide-containing contryphans from the venom of cone snails using de novo mass spectrometry-based sequencing methods.
abstract: We identified 12 short single disulfide-containing conopeptides from the venom of Conus coronatus, C. leopardus, C. lividus and C. zonatus. Interestingly, we detected the shortest contryphan sequence thus far characterized which contains only six amino acid residues. We also identified three distinct contryphan sequences of C. lividus without any proline residues and one sequence with an unusual post-translational modification (bromination of tryptophan). Furthermore, we characterized venom peptides of C. zonatus for the first time.
authors.affiliation: Andaman and Nicobar Centre for Ocean Science and Technology, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Dollygunj, Port Blair 744103, India. Electronic address: benjamin@niot.res.in.
authors.forename: Jayaseelan Benjamin
authors.initials: JB
authors.lastname: Franklin
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Rajaian Pushpabai
authors.initials: RP
authors.lastname: Rajesh
authors.affiliation: Andaman and Nicobar Centre for Ocean Science and Technology, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Dollygunj, Port Blair 744103, India.
authors.forename: Nambali Valsalan
authors.initials: NV
authors.lastname: Vinithkumar
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Pallikaranai, Chennai 600100, India.
authors.forename: Ramalingam
authors.initials: R
authors.lastname: Kirubagaran
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D052078:Conus Snail; D013058:Mass Spectrometry; D008978:Mollusk Venoms / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*
chemical_list: D008978:Mollusk Venoms; D010456:Peptides, Cyclic; C102355:contryphan
keywords: Cone snails; Conopeptides; Contryphans; Linear peptides; Mass spectrometry; Short conopeptides