title: Verification and fine mapping of qGW1.05, a major QTL for grain weight in maize (Zea mays L.).
abstract: Grain weight, one of the important factors to determine corn yield, is a typical quantitative inheritance trait. However, the molecular genetic basis of grain weight still remains limited. In our previous researches, a major QTL associated with grain weight, qGW1.05, has been identified between SSR markers umc1601 and umc1754 at bin locus 1.05-1.06 in maize. Here, its genetic and environmental stabiliteis were verified using a BC3F2population to identify the effect of qGW1.05 on grain weight. Further, qGW1.05-NILs were obtained by MAS successfully. Via a large BC6F2segregation population, together with polymorphic microsatellite markers developed between the parents to screen the genotype of the recombinant plants, qGW1.05 was positioned to a 1.11 Mb genome interval. Furthermore, the progenies of 15 recombinants were tested to confirm the effect of qGW1.05 on grain weight. Combining collinearity among cereal crops and genome annotation, the several candidate genes taking part in grain development were identified in the qGW1.05 region. In this study, qGW1.05 was limited to a 1.11 Mb region on chromosome 1, which established the foundation for understanding the molecular basis underlying kernel development and improving grain weight through MAS using the tightly flanking molecular markers in maize.
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
authors.forename: Huanqing
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Hu
authors.affiliation: College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China. yuling_li@126.com.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Li
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018556:Crops, Agricultural / Q000235:genetics; D008040:Genetic Linkage; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics*; D012639:Seeds / Q000502:physiology*; D003313:Zea mays / Q000235:genetics*
keywords: Fine mapping; Grain weight; Maize; NIL; QTL