title: Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism.
abstract: Adseverin is an actin-severing/capping protein that may contribute to osteoclast differentiation in vitro but its role in bone remodeling of healthy animals is not defined. We analyzed bone and osteoclast structure in adseverin conditional null mice at alveolar and long bone sites. In wild-type and adseverin null mice, as measured by dual-energy X-ray absorptiometry, there were no differences of bone mineral content or bone mineral density, indicating no change of bone metabolism. In tibiae, TRAcP+osteoclasts were formed in comparable numbers in adseverin null and wild-type mice. Ultrastructural analysis showed normal and similar abundance of ruffled borders, sealing zones, and mitochondria, and with no difference of osteoclast nuclear numbers. In contrast, analyses of long bone showed that in the absence of adseverin osteoclasts were smaller (120 ± 13 vs. 274 ± 19 µm2; p < 0.05), as were nuclear size and the surface area of cytoplasm. The nuclei of adseverin null osteoclasts exhibited more heterochromatin (31 ± 3%) than wild-type cells (8 ± 1%), suggesting that adseverin affects cell differentiation. The data indicate that in healthy, developing tissues, adseverin contributes to the regulation of osteoclast structure but not to bone metabolism in vivo.
authors.affiliation: Department of Oral Cell Biology and Functional Anatomy, Academic Centre for Dentistry Amsterdam (ACTA), Research Institute MOVE, University of Amsterdam and VU University Amsterdam, 11N-43, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands. y.cao@acta.nl.
authors.forename: Yixuan
authors.identifier: 0000-0002-6025-637X
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oral Cell Biology and Functional Anatomy, Academic Centre for Dentistry Amsterdam (ACTA), Research Institute MOVE, University of Amsterdam and VU University Amsterdam, 11N-43, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
authors.forename: Sara
authors.initials: S
authors.lastname: Sprangers
authors.affiliation: Department of Cell Biology and Histology, Core Facility Cellular Imaging, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Daisy I
authors.initials: DI
authors.lastname: Picavet
authors.affiliation: Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Glogauer
authors.affiliation: Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: McCulloch
authors.affiliation: Department of Oral Cell Biology and Functional Anatomy, Academic Centre for Dentistry Amsterdam (ACTA), Research Institute MOVE, University of Amsterdam and VU University Amsterdam, 11N-43, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
authors.forename: Vincent
authors.initials: V
authors.lastname: Everts
mesh_terms: D015502:Absorptiometry, Photon / Q000379:methods; D000818:Animals; D015519:Bone Density / Q000502:physiology; D001862:Bone Resorption / Q000235:genetics / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D018260:Gelsolin / Q000172:deficiency*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism*
chemical_list: D018260:Gelsolin; D008840:Microfilament Proteins; C065395:scinderin
keywords: Adseverin; Morphology; Osteoclast; Transmission electron microscopy