title: Wound Healing Activity of Extracts and Formulations ofAloe vera, Henna,Adiantum capillus-veneris, and Myrrh on Mouse Dermal Fibroblast Cells.
abstract: BACKGROUND
  Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such asAdiantum capillus-veneris,Commiphora molmol,Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts.
  
  
  METHODS
  Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of theTgfβ1 andVegf-Agenes were monitored by real-time polymerase chain reaction.
  
  
  RESULTS
  A. capillus-veneris,C. molmoland henna extract improved the expression ofTgfβ1gene. All used extracts upregulated the expression ofVegf-Agene and promoted the migration of mouse fibroblast cellsin vitro.
  
  
  CONCLUSIONS
  The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression ofTgfβ1andVegf-Agenes in fibroblast cells treated with extracts.
authors.affiliation: Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Negahdari
authors.affiliation: Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Galehdari
authors.affiliation: Department of Biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Kesmati
authors.affiliation: Department of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Anahita
authors.initials: A
authors.lastname: Rezaie
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Shariati
keywords: Fibroblast; herbal; transforming growth factor-β1; vascular endothelial growth factor; wound healing