title: The MicroRNA miR-210 Is Expressed by Cancer Cells but Also by the Tumor Microenvironment in Triple-Negative Breast Cancer.
abstract: The triple-negative breast cancer (TNBC) subtype occurs in about 15% of breast cancer and is an aggressive subtype of breast cancer with poor outcome. Furthermore, treatment of patients with TNBC is more challenging due to the heterogeneity of the disease and the absence of well-defined molecular targets. Microribonucleic acid (RNA) represents a new class of biomarkers that are frequently dysregulated in cancer. It has been described that the microRNA miR-210 is highly expressed in TNBC, and its overexpression had been linked to poor prognosis. TNBC are often infiltrated by immune cells that play a key role in cancer progression. The techniques traditionally used to analyze miR-210 expression such as next generation sequencing or quantitative real-time polymerase chain reaction (PCR) do not allow the precise identification of the cellular subtype expressing the microRNA. In this study, we have analyzed miR-210 expression by in situ hybridization in TNBC. The miR-210 signal was detected in tumor cells, but also in the tumor microenvironment, in a region positive for the pan-leucocyte marker CD45-LCA. Taken together, our results demonstrate that miR-210 is expressed in tumor cells but also in the tumor microenvironment. Our results also highlight the utility of using complementary approaches to take into account the cellular context of microRNA expression.
authors.affiliation: Laboratory of Translational Oncology (IB, SS, PD), Grand Hôpital de Charleroi/Institute of Pathology and Genetics, Gosselies, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bar
authors.affiliation: IPG-Biobank (AM, FA-S, ABA), Gosselies, Belgium.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Merhi
authors.affiliation: IPG-Biobank (AM, FA-S, ABA), Gosselies, Belgium.
authors.forename: Fadi
authors.initials: F
authors.lastname: Abdel-Sater
authors.affiliation: IPG-Biobank (AM, FA-S, ABA), Gosselies, Belgium.
authors.forename: Abduelhakem
authors.initials: A
authors.lastname: Ben Addi
authors.affiliation: Laboratory of Translational Oncology (IB, SS, PD), Grand Hôpital de Charleroi/Institute of Pathology and Genetics, Gosselies, Belgium.
authors.forename: Sara
authors.initials: S
authors.lastname: Sollennita
authors.affiliation: Service of Oncology-Haematology (J-LC), Grand Hôpital de Charleroi, Charleroi, Belgium.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Canon
authors.affiliation: Laboratory of Translational Oncology (IB, SS, PD), Grand Hôpital de Charleroi/Institute of Pathology and Genetics, Gosselies, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: Delrée
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D064726:Triple Negative Breast Neoplasms / Q000175:diagnosis / Q000235:genetics*; D059016:Tumor Microenvironment / Q000235:genetics*
chemical_list: C543800:MIRN210 microRNA, human; D035683:MicroRNAs
keywords: TNBC; in situ hybridization; miR-210; tumor microenvironment