title: The uniform structure of O-polysaccharides isolated from Dickeya solani strains of different origin.
abstract: O-polysaccharides were isolated from lipopolysaccharides obtained from four different strains of plant pathogenic bacteria belonging to the species Dickeya solani: two of them were isolated in Poland (IFB0099 and IFB0158), the third in Germany (IFB0223) and the last one, D. solani Type Strain IPO2222, originated from the Netherlands. In addition, the O-polysaccharide of a closely related species D. dadantii strain 3937 was isolated. The purified polysaccharides of the five strains were analyzed using NMR spectroscopy and chemical methods. Sugar and methylation analyses, including absolute configuration assignment, together with NMR data revealed that all O-polysaccharides tested are homopolymers of 6-deoxy-d-altrose (d-6dAlt) the following structure: →2)-β-d-6dAltp-(1→.
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Ossowska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czerwicka
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology University of Gdansk, Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Sledz
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology University of Gdansk, Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Zoledowska
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology University of Gdansk, Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Motyka
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology University of Gdansk, Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Golanowska
authors.affiliation: Univ Lyon, Université Lyon 1, INSA de Lyon, CNRS UMR5240 Microbiologie Adaptation et Pathogénie, F-69622, Villeurbanne, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Condemine
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology University of Gdansk, Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Lojkowska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland. Electronic address: zbigniew.kaczynski@ug.edu.pl.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kaczyński
mesh_terms: D004755:Enterobacteriaceae / Q000737:chemistry*; D019081:O Antigens / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D019081:O Antigens
keywords: 6-Deoxy-d-altrose; Dickeya dadantii; Dickeya solani; NMR; O-polysaccharide; Structure