title: Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping.
abstract: Colorectal cancer (CRC) is an aggressive human malignancy with a complex genomic landscape harboringKRASmutations. In 40%-60% of patients with CRC, constantly activeKRASproteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor (EGFR) agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the mainKRASmutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing.
authors.affiliation: Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy. maria.guarnaccia@cnr.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Guarnaccia
authors.affiliation: Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy. iemmolo.rosario@gmail.com.
authors.forename: Rosario
authors.initials: R
authors.lastname: Iemmolo
authors.affiliation: STMicroelectronics, Stradale Primosole, 50-95121 Catania, Italy. sabrina.conoci@st.com.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Petralia
authors.affiliation: STMicroelectronics, Stradale Primosole, 50-95121 Catania, Italy. sabrina.conoci@st.com.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Conoci
authors.affiliation: Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy. sebastiano.cavallaro@cnr.it.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Cavallaro
mesh_terms: D000970:Antineoplastic Agents; D015179:Colorectal Neoplasms; D066246:ErbB Receptors; D005838:Genotype; D006801:Humans; D009154:Mutation; D016283:Proto-Oncogene Proteins p21(ras); D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; C117307:KRAS protein, human; C512478:EGFR protein, human; D066246:ErbB Receptors; D016283:Proto-Oncogene Proteins p21(ras)
keywords: Genome-based test; colorectal cancer; diagnostics; qPCR