title: Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles.
abstract: The effect of citrate-stabilized gold nanoparticles (AuNPs) on the secondary structure of an artificial β-sheet-forming cationic peptide has been studied. The AuNPs inhibited β-sheet formation and led to fragmented fibrils and spherical oligomers with assembled AuNPs on their surface. Besides this structural change, the functional properties of the peptide are also different. Whereas the peptide was unable to act as a vector for gene delivery, formation of a complex with AuNPs allowed successful gene delivery into cells.
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117, Essen, Germany.
authors.forename: Poulami
authors.initials: P
authors.lastname: Jana
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117, Essen, Germany.
authors.forename: Krishnananda
authors.initials: K
authors.lastname: Samanta
authors.affiliation: Institute for Biology, University of Duisburg-Essen, 45117, Essen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Bäcker
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117, Essen, Germany.
authors.forename: Elio
authors.initials: E
authors.lastname: Zellermann
authors.affiliation: Institute for Biology, University of Duisburg-Essen, 45117, Essen, Germany.
authors.forename: Shirley
authors.initials: S
authors.lastname: Knauer
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117, Essen, Germany.
authors.forename: Carsten
authors.identifier: 0000-0001-6062-0357
authors.initials: C
authors.lastname: Schmuck
keywords: amyloid fibers; beta sheet formation; gene delivery; nanoparticles; self-assembly