title: B7-H3 Promotes the Migration and Invasion of Human Bladder Cancer Cells via the PI3K/Akt/STAT3 Signaling Pathway.
abstract: Bladder cancer is one of most common malignant cancer. Although previous studies have found abnormal expression of B7-H3 in human bladder cancer tissues, the exact role and molecular mechanism of B7-H3 in bladder cancer remain unknown. In this study, we first detected the expression of B7-H3 in human bladder cancer samples and cell lines, and analyzed its correlations with clinicopathological pathological parameters. Next, siRNAs or overexpression plasmids of B7-H3 were transfected into T24 or 5637 cells, and cell proliferation, apoptosis, migration and invasion were analyzed via CCK-8, colony formation, flow cytometry and transwell assays, protein expression levels were determined by western blotting. The results presented here showed B7-H3 was upregulated in bladder cancer samples compared with normal tissues, and the expression level was correlated with local invasion status. B7-H3 did not affect cell proliferation and apoptosis, but cell migration and invasion were changed through the regulation of matrix metalloproteinase (MMP) 2/9. Knockdown of B7-H3 resulted in decreased activity of the STAT3 and PI3K/Akt pathways, and the Akt served as an upstream regulator of the STAT3. Our results suggest that the overexpression of B7-H3 promotes the migration and invasion of human bladder cancer cells through the PI3K/Akt/STAT3 signaling pathway.
authors.affiliation: Department of Cell Biology, Third Military Medical University, Chongqing 400038, China;; Trainee Brigade, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Guoning
authors.initials: G
authors.lastname: Guo
authors.affiliation: Trainee Brigade, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.affiliation: Trainee Brigade, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Cell Biology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Urology Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Cell Biology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Cell Biology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Qiangguo
authors.initials: Q
authors.lastname: Gao
keywords: B7-H3; MMP; PI3K/Akt/STAT3; human bladder cancer; invasion; migration