title: The Effect of Phototherapy on Cancer Predisposition Genes of Diabetic and Normal Human Skin Fibroblasts.
abstract: The purpose of this study was to investigate whether LED light at different wavelengths affects the expression profile of 143 cancer predisposition genes in both diabetic and normal human fibroblasts. In this study, both diabetic and normal fibroblast cell lines were cultured and irradiated with red (635 nm), green (520 nm), and blue (465 nm) LED light for 10 minutes at 0.67 J/cm2each. After that, mRNA from all cell lines was extracted for microarray analysis. We found that green light activates EPHB2, KIT, ANTXR2, ESCO2, MSR1, EXT1, TSC1, KIT, NF1, BUB1B, FANCD2, EPCAM, FANCD2, NF, DIS3L2, and RET in normal fibroblast cells, while blue and red light can upregulate RUNX1, PDGFRA, EHBP1, GPC3, AXIN2, KDR, GLMN, MSMB, EPHB2, MSR1, KIT, FANCD2, BMPR1A, BUB1B, PDE11A, and RET. Therefore, genetic screening before phototherapy treatment may be required.
authors.affiliation: Biomedical Engineering Institute, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
authors.forename: Pongsathorn
authors.identifier: 0000-0002-3588-4577
authors.initials: P
authors.lastname: Chotikasemsri
authors.affiliation: Department of Orthopedic Surgery and Physical Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
authors.forename: Boonsin
authors.initials: B
authors.lastname: Tangtrakulwanich
authors.affiliation: Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
authors.forename: Surasak
authors.initials: S
authors.lastname: Sangkhathat
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics* / Q000528:radiation effects; D003920:Diabetes Mellitus / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D020022:Genetic Predisposition to Disease; D006801:Humans; D008027:Light / Q000009:adverse effects; D046228:Microarray Analysis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010789:Phototherapy / Q000009:adverse effects*; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects
chemical_list: D009363:Neoplasm Proteins