title: Internal validation of the DNAscan/ANDE™ Rapid DNA Analysis™ platform and its associated PowerPlex®16 high content DNA biochip cassette for use as an expert system with reference buccal swabs.
abstract: Rapid DNA platforms are fully integrated systems capable of producing and analyzing short tandem repeat (STR) profiles from reference sample buccal swabs in less than two hours. The technology requires minimal user interaction and experience making it possible for high quality profiles to be generated outside an accredited laboratory. The automated production of point of collection reference STR profiles could eliminate the time delay for shipment and analysis of arrestee samples at centralized laboratories. Furthermore, point of collection analysis would allow searching against profiles from unsolved crimes during the normal booking process once the infrastructure to immediately search the Combined DNA Index System (CODIS) database from the booking station is established. The DNAscan/ANDE™ Rapid DNA Analysis™ System developed by Network Biosystems was evaluated for robustness and reliability in the production of high quality reference STR profiles for database enrollment and searching applications. A total of 193 reference samples were assessed for concordance of the CODIS 13 loci. Studies to evaluate contamination, reproducibility, precision, stutter, peak height ratio, noise and sensitivity were also performed. The system proved to be robust, consistent and dependable. Results indicated an overall success rate of 75% for the 13 CODIS core loci and more importantly no incorrect calls were identified. The DNAscan/ANDE™ could be confidently used without human interaction in both laboratory and non-laboratory settings to generate reference profiles.
authors.affiliation: DNA Support Unit, FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, United States; Rapid DNA Program Office, FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, United States. Electronic address: Lilliana.Moreno@ic.fbi.gov.
authors.forename: Lilliana I
authors.initials: LI
authors.lastname: Moreno
authors.affiliation: DNA Support Unit, FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, United States.
authors.forename: Alice L
authors.initials: AL
authors.lastname: Brown
authors.affiliation: Rapid DNA Program Office, FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, United States.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Callaghan
mesh_terms: D004247:DNA / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000295:instrumentation*; D030561:Databases, Nucleic Acid; D053803:Forensic Genetics; D006801:Humans; D018895:Microsatellite Repeats; D009061:Mouth Mucosa / Q000737:chemistry; D060885:Multiplex Polymerase Chain Reaction / Q000295:instrumentation*; D015203:Reproducibility of Results
chemical_list: D004247:DNA
keywords: Arrestee DNA collection; Booking station; CODIS; DNAscan/ANDE™ Rapid DNA Analysis™ system; PowerPlex 16(®); Rapid DNA