title: Chiral recognition and determination of enantiomeric excess by mass spectrometry: A review.
abstract: Chiral analysis is of great importance to fundamental and applied research in chemical, biological and pharmaceutical sciences. Due to the superiority of mass spectrometry (MS) over other analytical methods in terms of speed, specificity and sensitivity, chiral analysis by MS has attracted much interest in recent years. Chiral analysis by MS typically involves introduction of a chiral selector to form diastereomers with analyte enantiomers, and comparison of the behaviors of diastereomers in MS. Chiral differentiation can be achieved by comparing the relative abundances of diastereomers, the thermodynamic or kinetic constants of ion-molecule reactions of diastereomers in the gas phase, the dissociation of diastereomers in MS/MS, or the mobility of diastereomers in ion mobility mass spectrometry. In this review, chiral recognition and determination of enantiomeric excess by these chiral MS methods were summarized, and the prospects of chiral analysis by MS were discussed.
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, Guangdong, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of Hong Kong Polytechnic University, Shenzhen 518057, China.
authors.forename: Xiangying
authors.initials: X
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of Hong Kong Polytechnic University, Shenzhen 518057, China; Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules (Yanbian University), Ministry of Education, Yanji 133002, Jilin, China; State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China. Electronic address: zhongping.yao@polyu.edu.hk.
authors.forename: Zhong-Ping
authors.initials: ZP
authors.lastname: Yao
mesh_terms: D000596:Amino Acids / Q000032:analysis; D007700:Kinetics; D004364:Pharmaceutical Preparations / Q000032:analysis; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D013816:Thermodynamics
chemical_list: D000596:Amino Acids; D004364:Pharmaceutical Preparations
keywords: Chiral recognition; Enantiomeric excess (ee); Ion mobility; Mass spectrometry