title: Silence of Long Noncoding RNA NEAT1 Inhibits Malignant Biological Behaviors and Chemotherapy Resistance in Gastric Cancer.
abstract: Gastric cancer (GC) is the most common solid tumor in digestive system. Nuclear-enriched abundant transcript 1 (NEAT1) gene is a lncRNA, and reveal potential oncogene role in several malignant tumors. The aim of this study is to investigate the expression and clinical significance of Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) gene and its influence to malignant biologic behaviors and chemotherapy resistance to adriamycin in GC. This study found NEAT1 was up-regulated in GC tissues and cells, especially in in GC adriamycin-resistant cells. NEAT1 silence in SGC7901 cells could inhibit proliferation and invasion ability, and promote cell apoptosis significantly. NEAT1 silence in adriamycin-resistant SGC7901/ADR cells also depressed the half maximal inhibitory concentration (IC50) for adriamycin, chemotherapy resistance to adriamycin was inhibited significantly. NEAT1 knockdown promoted apoptosis in SGC7901/ADR cells induced by adriamycin. In summary, lncRNA NEAT1 is high-expressed in GC and functions as an oncogene to modulate apoptosis, invasion, proliferation and chemotherapy resistance of GC cells, which might be a novel potential therapeutic target for GC.
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China.
authors.forename: Bochao
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China.
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China.
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China.
authors.forename: Huimian
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Surgical Oncology, Affiliated First Hospital, China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning, 110001, China. huang_bj@163.com.
authors.forename: Baojun
authors.initials: B
authors.lastname: Huang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D014408:Biomarkers, Tumor / Q000235:genetics*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors* / Q000235:genetics; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D004317:Doxorubicin
keywords: Chemotherapy resistance; Gastric cancer; Long noncoding RNA; Malignant behaviors; NEAT1