title: Increased activities of peroxidase and polyphenol oxidase enhance cassava resistance to Tetranychus urticae.
abstract: In order to study the function of peroxidase (POD) and polyphenol oxidase (PPO) in cassava resistance to spider mites, we tested the changes of transcription levels and activities of these two protective enzymes in both cassava and Tetranychus urticae (=T. cinnabarinus) during the interaction. The results showed that after damage of the mite-susceptible cassava cultivar BRA900 by T. urticae for 1 and 8 days, the transcription levels of MePOD and MePPO and the activities of POD and PPO showed no significant difference compared with those in undamaged leaves. However, the corresponding transcription levels and activities in 1- and 8-day-damaged leaves of mite-resistant cassava cultivar C1115 increased to a significant level of approximately twofold. When T. urticae fed on BRA900 for 1 and 8 days, the transcription levels of TcPPO and TcPOD and the activities of PPO and POD showed no significant difference compared with those before feeding. However, the corresponding transcription levels and activities of these two protective enzymes in T. urticae feeding on C1115 significantly decreased by about half. This study preliminarily validates the function of POD and PPO in cassava resistance to T. urticae, and provides candidate gene resource for molecular breeding of spider mite-resistant cassava.
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China.|Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China.|Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China.|Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China. chqingztq@163.com.|Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China. chqingztq@163.com.|Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China. chqingztq@163.com.|Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China. chqingztq@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China.|Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China.|Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China.|Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China.|Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China.|Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China.|Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wu
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China.|Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China.|Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China.|Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China.
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China.|Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China.|Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China.|Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000378:metabolism*; D005247:Feeding Behavior; D002365:Manihot / Q000469:parasitology*; D008924:Mite Infestations / Q000469:parasitology; D009195:Peroxidase / Q000378:metabolism*; D010935:Plant Diseases / Q000469:parasitology; D040102:Tetranychidae / Q000254:growth & development*
chemical_list: D004156:Catechol Oxidase; D009195:Peroxidase
keywords: Cassava resistance; Peroxidase; Polyphenol oxidase; Tetranychus urticae