title: MiR-155 promotes cell proliferation and inhibits apoptosis by PTEN signaling pathway in the psoriasis.
abstract: MicroRNAs (miRNAs) have been demonstrated to contribute to malignant progression in psoriasis development. The purposes of the study was to evaluated the effects of miRNA-155 on cell proliferation, migration and apoptosis in psoriasis development via PTEN singaling pathway and identify its direct target protein. Quantitative real-time RT-PCR (qRT-PCR) was performed to examine the level of miR-155 in psoriasis cells, miR-155 was downregulated in a psoriasis cell line Hacat by transfected with small interfering RNA (siRNA), respectively. Cell survival was detected by the MTT assay and colony formation assay. Cell migration and invasion were measured via wound-healing assayand transwell assay. In addition, cell cycle and apoptosis about psoriasis cells was measured by flow cytometry. In this study, qRT-PCR assay showed that the expressions of miR-155 mRNA in psoriasis tissues were significantly higher than that in normal tissues. The assays about cell growth and proliferation showed that miR-155 knockdown led to a significant decrease in cell proliferation which was determined by MTT assay and colony formation assay compared to those of Lv-NC cells. Flow cytometry analysis showed that depletion of miR-155 could cause cell cycle change and the number of apoptotic cells was significantly increased in Lv-miR155 cells compared with control cells. In addition, the expression of several apoptosis-related factors were dramatically changed, such as PTEN, PIP3, AKT, p-AKT, Bax and Bcl-2. Our findings indicate that down-regulation of miR-155 significantly inhibits proliferation, migration, invasion and promotes apoptosis through PTEN singaling pathway in psoriasis cells. miR-155 might function as an oncogene miRNA in the progress of psoriasis.
authors.affiliation: The Second Affiliated Hospital of Soochow University, SuZhou, 215004, China.
authors.forename: Longjiang
authors.initials: L
authors.lastname: Xu
authors.affiliation: The Second Affiliated Hospital of Soochow University, SuZhou, 215004, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Leng
authors.affiliation: The Second Affiliated Hospital of Soochow University, SuZhou, 215004, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
authors.affiliation: The Second Affiliated Hospital of Soochow University, SuZhou, 215004, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Ji
authors.affiliation: The Second Affiliated Hospital of Soochow University, SuZhou, 215004, China. Electronic address: fujx_2016@sina.com.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Fu
authors.affiliation: The Second Affiliated Hospital of Soochow University, SuZhou, 215004, China. Electronic address: lenhong_2016@sina.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Leng
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D011565:Psoriasis / Q000235:genetics*; D034622:RNA Interference / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D051017:Apoptosis Regulatory Proteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: Apoptosis; MiR-155; Proliferation; Psoriasis; Signaling pathway