title: Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples.
abstract: In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic. In order to better evaluate the performance, it was compared with a conventional amperometric immunosensor, working with a competitive format with the aim of finding out experimental real advantages and disadvantages of two respective methods. Results showed that certain analytical features of the new SPR immunodevice, such as the lower limit of detection (LOD) value and the width of the linear range, are poorer than those of a conventional amperometric immunosensor, which adversely affects the application to samples such as natural waters. On the other hand, the SPR immunosensor was more selective to ampicillin, and measurements were more easily and quickly attained compared to those performed with the conventional competitive immunosensor.
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. mauro.tomassetti@uniroma1.it.
authors.forename: Mauro
authors.initials: M
authors.lastname: Tomassetti
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. gancetto2@hotmail.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Merola
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. elisabettamartini@libero.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Martini
authors.affiliation: Department of Chemistry, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. luigi.campanella@uniroma1.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Campanella
authors.affiliation: Department of Chemistry and Pharmacy Technology, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. gabriella.sanzo@uniroma1.it.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sanzò
authors.affiliation: Department of Chemistry and Pharmacy Technology, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. gabriele.favero@uniroma1.it.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.affiliation: Department of Chemistry and Pharmacy Technology, "Sapienza" University of Rome, P.le A. Moro, 5, 00185 Rome, Italy. franco.mazzei@uniroma1.it.
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
mesh_terms: D000667:Ampicillin; D000900:Anti-Bacterial Agents; D015374:Biosensing Techniques; D007118:Immunoassay; D020349:Surface Plasmon Resonance
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin
keywords: ampicillin; analytical comparison; competitive amperometric immunosensor; flow surface plasmon resonance (SPR) immunosensor