title: Co-production of chitin-glucan complex and xylitol by Komagataella pastoris using glucose and xylose mixtures as carbon source.
abstract: Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46-0.54g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64g/L, with a yield on xylose of 0.52g/g. Lower CGC production was reached as the xylose content was increased in the substrate mixtures, due to the lower biomass production. Moreover, cultivation in the presence of xylitol resulted in CGC enriched in chitin with higher molecular weight. These results suggest the possibility of using K. pastoris for the co-production of CGC and xylitol using glucose/xylose-rich substrates. It may also be a strategy to tailor CGC composition and average molecular weight.
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal. Electronic address: df.araujo@campus.fct.unl.pt.
authors.forename: Diana
authors.initials: D
authors.lastname: Araújo
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal. Electronic address: a4406@fct.unl.pt.
authors.forename: Filomena
authors.initials: F
authors.lastname: Freitas
authors.affiliation: Interfacultary Research Centre of Biomaterials (CEIB), University of Liège, B-4000 Liège, Belgium. Electronic address: csevrin@ulg.ac.be.
authors.forename: Chantal
authors.initials: C
authors.lastname: Sevrin
authors.affiliation: Interfacultary Research Centre of Biomaterials (CEIB), University of Liège, B-4000 Liège, Belgium. Electronic address: c.grandfils@ulg.ac.be.
authors.forename: Christian
authors.initials: C
authors.lastname: Grandfils
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal. Electronic address: amr@fct.unl.pt.
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Reis
mesh_terms: D002244:Carbon; D002686:Chitin / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis*; D005947:Glucose; D004718:Saccharomycetales / Q000378:metabolism*; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose
chemical_list: D003470:Culture Media; D005936:Glucans; D002686:Chitin; D002244:Carbon; D014994:Xylose; D005947:Glucose; D014993:Xylitol
keywords: Chitin-glucan complex (CGC); Glucose; Komagataella pastoris; Xylitol; Xylose