title: A biorefinery-based approach for the production of ethanol from enzymatically hydrolysed cotton stalks.
abstract: Cotton post-harvest residue/cotton stalk (CS) - a major agro-residue in south asian countries was evaluated as a feed stock for bioethanol production. The common thermochemical pretreatment strategies based on dilute acid and alkali and different combinations of biomass hydrolyzing enzymes were evaluated for saccharification of CS biomass. A hydrolytic efficiency of 80% was achieved for alkali treated biomass using cellulase supplemented with beta glucosidase. Recycling of undigested/residual biomass and/or enzyme supported same final sugar concentration as for fresh hydrolytic experiments. Fermentation was carried out using a novel, inhibitor-resistant strain of Saccharomyces cerevisiae where 76% of theoretical maximum efficiency was attained. Material balances were derived for the entire process from biomass pre-processing to hydrolysis.
authors.affiliation: Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Meera
authors.initials: M
authors.lastname: Christopher
authors.affiliation: Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Mathew
authors.affiliation: Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India.
authors.forename: M
authors.initials: M
authors.lastname: Kiran Kumar
authors.affiliation: Centre for Innovative and Applied Bioprocessing, Mohali 160 071, Punjab, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India. Electronic address: rajeevs@niist.res.in.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Sukumaran
mesh_terms: D018533:Biomass; D002480:Cellulase; D032561:Cotton Fiber; D000431:Ethanol; D005285:Fermentation; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae
chemical_list: D000431:Ethanol; D002480:Cellulase
keywords: Alkaline pre-treatment; Bioethanol; Biofuel; Cotton residue; Cotton stalk; Saccharomyces cerevisiae