title: Ethylene response factor AtERF72 negatively regulates Arabidopsis thaliana response to iron deficiency.
abstract: Ethylene regulates the plant's response to stress caused by iron (Fe) deficiency. However, specific roles of ERF proteins in response to Fe deficiency remain poorly understood. Here, we investigated the role of ERF72 in response to iron deficiency in Arabidopsis thaliana. In this study, the levels of the ethylene response factor AtERF72 increased in leaves and roots induced under the iron deficient conditions. erf72 mutant plants showed increased growth compared to wild type (WT) when grown in iron deficient medium for 5 d. erf72 mutants had increased root H+velocity and the ferric reductase activity, and increase in the expression of the iron deficiency response genes iron-regulated transporter 1 (IRT1) and H+-ATPase (HA2) levels in iron deficient conditions. Compared to WT plants, erf72 mutants retained healthy chloroplast structure with significantly higher Fe and Mg content, and decreased chlorophyll degradation gene pheophorbide a oxygenase (PAO) and chlorophyllase (CLH1) expression when grown in iron deficient media. Yeast one-hybrid analysis showed that ERF72 could directly bind to the promoter regions of iron deficiency responses genes IRT1, HA2 and CLH1. Based on our results, we suggest that ethylene released from plants under iron deficiency stress can activate the expression of ERF72, which responds to iron deficiency in the negative regulation.
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Han
authors.affiliation: Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing 100193, PR China; Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, PR China. Electronic address: xuefengx@cau.edu.cn.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Xu
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins; D007501:Iron / Q000378:metabolism*; D010935:Plant Diseases; D018515:Plant Leaves / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000607352:RAP2.3 protein, Arabidopsis; D014157:Transcription Factors; D007501:Iron
keywords: Arabidopsis thaliana; Chlorophyll degradation; ERF72; Iron uptake; Negative regulation