title: Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction.
abstract: The detection of mycotoxin contamination in foodstuffs is highly significant for public health. Herein we report an analytical method based on magnetic solid-phase extraction (MSPE) and UPLC-MS/MS for the simultaneous determination of mycotoxins, including fumonisins B1(FB1), zearalenone (ZON) and ochratoxin A (OTA), in vegetable oil. Magnetic nanoparticles coated with double layers of silicon dioxide were synthesised and found to be an effective MSPE adsorbent for mycotoxins. The proposed MSPE procedure serves not only for sample clean-up but also for mycotoxin enrichment that enhances greatly the assay's sensitivity. Under the selected MSPE conditions, linear matrix-matched calibration curves were obtained for mycotoxins in a concentration range from 0.178 to 625 μg kg-1. The limits of detection were 0.210 μg kg-1for FB1, 0.0800 μg kg-1for OTA and 1.03 μg kg-1for ZON. The proposed MSPE UPLC-MS/MS method was applied for the determination of mycotoxins in vegetable oil samples, including maize oil, rapeseed oil and soybean oil. ZON was detected in a maize oil at 101 μg kg-1, which is below the European Union limit of 200 μg kg-1in foodstuffs.
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.|c University of Chinese Academy of Sciences , Beijing , China.|d Sichuan Willtest Technology Co., Ltd , Chengdu , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wan
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Bai
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.|b Department of Chemistry and Biochemistry , Jackson State University , Jackson , MS , USA.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: d Sichuan Willtest Technology Co., Ltd , Chengdu , China.
authors.forename: Feng-Ping
authors.initials: FP
authors.lastname: Zhang
authors.affiliation: d Sichuan Willtest Technology Co., Ltd , Chengdu , China.
authors.forename: Yao-Min
authors.initials: YM
authors.lastname: Liu
authors.affiliation: a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.
authors.forename: Xun
authors.initials: X
authors.lastname: Liao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008280:Magnetics; D009183:Mycotoxins / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D009183:Mycotoxins; D010938:Plant Oils
keywords: UPLC-MS/MS; Vegetable oil; fumonisin B1; magnetic solid-phase extraction (MSPE); ochratoxin A; zearalenone