title: Synthesis of macromolecular mimics of small leucine-rich proteoglycans with a poly(ethylene glycol) core and chondroitin sulphate bristles.
abstract: Small leucine-rich proteoglycans (SLRPs) are a class of molecules prevalent in almost all tissues types and are thought to be responsible for collagen organization and macro-scale biological properties. However, when they are dysfunctional or degraded, severe pathological phenotypes are observed. Here we investigate macromolecular mimics to SLRPs using poly(ethylene glycol) (PEG) as a core (replacing the protein core of natural SLRPs) and chondroitin sulphate (CS) bristle(s) in an end-on attachment (via epoxide-amine reactions), mimicking the physical structure of the natural SLRPs. Poly(ethylene glycol)-diglycidyl ether (PEG-DEG) and ethylene glycol-diglycidyl ether (EG-DGE) monomers were used to incorporate CS bristles into a macromolecule that closely mimics the SLRP biglycan structure in a grafting-to strategy. The kinetics of these reactions was studied along with the specific viscosity and cytocompatibility of resulting CS macromolecules. Structures were found to incorporate two CS chains (similar to biglycan) on average and exhibited cytocompatibility equivalent to or better than CS-only controls.
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut St, Philadelphia, PA 19104-2816, United States.
authors.forename: Sumona
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut St, Philadelphia, PA 19104-2816, United States.
authors.forename: Carli
authors.initials: C
authors.lastname: Moorehead
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut St, Philadelphia, PA 19104-2816, United States.
authors.forename: Katsiaryna
authors.initials: K
authors.lastname: Prudnikova
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut St, Philadelphia, PA 19104-2816, United States.
authors.forename: Caroline L
authors.initials: CL
authors.lastname: Schauer
authors.affiliation: Department of Chemistry, Drexel University, 3141 Chestnut St, Philadelphia, PA 19104-2816, United States.
authors.forename: Lynn S
authors.initials: LS
authors.lastname: Penn
authors.affiliation: Department of Materials Science and Engineering, Drexel University, 3141 Chestnut St, Philadelphia, PA 19104-2816, United States. Electronic address: marcolms@drexel.edu.
authors.forename: Michele
authors.initials: M
authors.lastname: Marcolongo
mesh_terms: D058578:Biglycan; D002809:Chondroitin Sulfates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D000071232:Small Leucine-Rich Proteoglycans / Q000138:chemical synthesis*
chemical_list: D058578:Biglycan; D000071232:Small Leucine-Rich Proteoglycans; D011092:Polyethylene Glycols; D002809:Chondroitin Sulfates
keywords: Biomimetic; Chondroitin sulphate; Epoxide; Extracellular matrix; Small leucine-rich proteoglycans (SLRP)