title: Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration.
abstract: TCTP (Translationally Controlled Tumour Protein) is a multifunctional protein that plays a role in the development, immune system, tumour reversion, and maintenance of stem cells. The mRNA of the Tpt1 gene is over-expressed during liver regeneration. But, the function of the protein in regeneration is not known. To study the role of the protein in regeneration, the earthworm Eudrilus eugeniae was chosen. First, the full length cDNA of the Tpt1 gene was sequenced. The size of the cDNA is 504 bp and the protein has 167 amino acids. The highest level of TCTP expression was documented in the worm after three days of regeneration. The protein was found to be expressed specifically in the epithelial layer of the skin. During regeneration, the protein expression was found to be the highest at the tip of blastema. The pharmacological suppression of TCTP using nutlin-3 and TCTP RNAi experiments resulted in the failure of the regeneration process. The suppression of TCTP caused the arrest of proliferation in posterior amputated worms. The severe cell death was documented in the amputated region of nutlin-3 injected worm. The silencing of TCTP has blocked the modification of clitellar segments. The experiments confirm that TCTP has major functions in the upstream signalling of cell proliferation in the early regeneration process in E. eugeniae.
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Elaiya Raja
authors.initials: ER
authors.lastname: Subramanian
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Nino
authors.initials: N
authors.lastname: Gopi Daisy
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Dinesh Kumar
authors.initials: DK
authors.lastname: Sudalaimani
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Kalidas
authors.initials: K
authors.lastname: Ramamoorthy
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Subburathinam
authors.initials: S
authors.lastname: Balakrishnan
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Jackson Durairaj
authors.initials: JD
authors.lastname: Selvan Christyraj
authors.affiliation: Viral Hepatitis and Gene/Cell Therapy Laboratory, CSMC Viral Vector Core, University of California, Los Angeles, United States of America.
authors.forename: Vaithilingaraja
authors.initials: V
authors.lastname: Arumugaswami
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.
authors.forename: Sudhakar
authors.identifier: http://orcid.org/0000-0002-1648-5474
authors.initials: S
authors.lastname: Sivasubramaniam
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D015870:Gene Expression; D008938:Mitosis; D009835:Oligochaeta / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D012038:Regeneration
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering