title: Reference Values for the Revised Anti-Müllerian Hormone Generation II Assay: Infertile Population-based Study.
abstract: Anti-Müllerian hormone (AMH) is now accepted as an important clinical marker of ovarian reserve and is increasingly measured as an initial evaluation at infertility clinics. The aim of this study was to establish reference values for the revised second generation (Gen II) assay using population-based data. In this population-based cohort study, AMH data from unselected infertile women aged 25-45 years from June 2013 to June 2014 (n = 15,801) were collected. The AMH values were measured using the revised Gen II assay. We established and validated 5 AMH-age regression models. Based on the optimal AMH-age model, reference values and centile charts were obtained. The quadratic model (log AMH = 0.410 × age -0.008 × age² -3.791) was the most appropriate for describing the age-dependent decrease in AMH measured using the revised Gen II assay. This is the largest population-based study to establish age-specific reference values of AMH using the revised Gen II assay. These reference values may provide more specific information regarding the ovarian reserve estimation of infertile women.
authors.affiliation: Hamchoon Women's Clinic, Seoul, Korea.
authors.forename: Joong Yeup
authors.identifier: http://orcid.org/0000-0002-1536-3811
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Medical Research Collaborating Center, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Soyeon
authors.identifier: http://orcid.org/0000-0003-3440-2027
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Jung Ryeol
authors.identifier: http://orcid.org/0000-0003-3743-2934
authors.initials: JR
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Byung Chul
authors.identifier: http://orcid.org/0000-0003-2289-6090
authors.initials: BC
authors.lastname: Jee
authors.affiliation: Hamchoon Women's Clinic, Seoul, Korea.
authors.forename: Chung Hyon
authors.identifier: http://orcid.org/0000-0001-7643-2975
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Samkwang Medical Laboratories, Seoul, Korea.
authors.forename: Soyeon
authors.identifier: http://orcid.org/0000-0001-8216-6562
authors.initials: S
authors.lastname: Seo
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Chang Suk
authors.identifier: http://orcid.org/0000-0003-1835-7350
authors.initials: CS
authors.lastname: Suh
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. seokhyun@snu.ac.kr.
authors.forename: Seok Hyun
authors.identifier: http://orcid.org/0000-0003-0649-3224
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000328:Adult; D000367:Age Factors; D054304:Anti-Mullerian Hormone / Q000032:analysis* / Q000592:standards; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D006801:Humans; D016014:Linear Models; D008875:Middle Aged; D065851:Ovarian Reserve / Q000502:physiology; D011933:Reagent Kits, Diagnostic; D012016:Reference Values
chemical_list: D011933:Reagent Kits, Diagnostic; D054304:Anti-Mullerian Hormone
keywords: AMH; AMH Generation II Assay; Ovarian Reserve; Reference Values