title: Elevated serum levels of TNF-α, IL-8, and ECP can be involved in the development and progression of bronchial asthma.
abstract: This study aimed to explore the value of elevated serum levels of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), and eosinophil cationic protein (ECP) in the diagnosis of bronchial asthma (BA).
  
  
  
  A total of 170 patients with BA (case group, 85 patients in acute attack and 85 patients in clinical remission) and 150 healthy individuals (control group) were enrolled in this study. Enzyme-linked immunosorbent assay and receiver operating characteristic (ROC) curves were calculated for the contents and diagnostic values of serum TNF-α, IL-8, and ECP in BA.
  
  
  
  Compared with the control group, patients in acute attack and clinical remission had higher TNF-α, IL-8, and ECP levels (p < 0.05). The serum level of TNF-α was positively correlated with IL-8 and ECP (p < 0.05). ROC curves showed that the diagnostic threshold value of IL-8 was 13.53 ng/ml, its area under the curve (AUC) was 0.87, its specificity was 99.3%, and its sensitivity was 57.6%. The diagnostic threshold value of TNF-α was 1.29 ng/ml with AUC being 0.94, specificity was 89.3%, and sensitivity was 83.5%. ECP showed 7.22 ng/ml diagnostic threshold value (AUC = 0.88, specificity = 74.0%, sensitivity = 86.5%). The FEV1/pre(%) and FEV1/FVC were negatively correlated and the Z5/pre(%) and resonance frequency (Fres) were positively correlated with the serum levels of TNF-α, IL-8, and ECP in patients in acute attack and in clinical remission (all p < 0.05).
  
  
  
  Our findings reveal that elevated serum levels of TNF-α, IL-8, and ECP can be involved in the development and progression of BA.
authors.affiliation: a Department of Biochemistry and Molecular Biology , Xi'an Jiaotong University College of Medicine , Xi'an , P. R. China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Jiang
authors.affiliation: a Department of Biochemistry and Molecular Biology , Xi'an Jiaotong University College of Medicine , Xi'an , P. R. China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Yang
authors.affiliation: b Xi'an Public Health Bureau , Xi'an , P. R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Lv
authors.affiliation: c Department of Surgery , The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , P. R. China.
authors.forename: Peng-Hui
authors.initials: PH
authors.lastname: Zhuang
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood* / Q000209:etiology / Q000503:physiopathology; D047150:Eosinophil Cationic Protein / Q000097:blood*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D014797:Vital Capacity
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D047150:Eosinophil Cationic Protein
keywords: Bronchial asthma; Interleukin-8; eosinophil cationic protein; tumor necrosis factor-α