title: Cannabidiol reduces lung injury induced by hypoxic-ischemic brain damage in newborn piglets.
abstract: BackgroundBrain hypoxic-ischemic (HI) damage induces distant inflammatory lung damage in newborn pigs. We aimed to investigate the effects of cannabidiol (CBD) on lung damage in this scenario.MethodsNewborn piglets received intravenous vehicle, CBD, or CBD+WAY100635 (5-HT1Areceptor antagonist) after HI brain damage (carotid flow interruption and FiO20.10 for 30 min). Total lung compliance (TLC), oxygenation index (OI), and extravascular lung water content (EVLW) were monitored for 6 h. Histological damage, interleukin (IL)-1β concentration, and oxidative stress were assessed in brain and lung tissue. Total protein content was determined in bronchoalveolar lavage fluid (BALF).ResultsCBD prevented HI-induced deleterious effects on TLC and OI and reduced lung histological damage, modulating inflammation (decreased leukocyte infiltration and IL-1 concentration) and reducing protein content in BALF and EVLW. These effects were related to CBD-induced anti-inflammatory changes in the brain. HI did not increase oxidative stress in the lungs. In the lungs, WAY100635 blunted the beneficial effects of CBD on histological damage, IL-1 concentration, and EVLW.ConclusionsCBD reduced brain HI-induced distant lung damage, with 5-HT1Areceptor involvement in these effects. Whether the effects of CBD on the lungs were due to the anti-inflammatory effects on the brain or due to the direct effects on the lungs remains to be elucidated.
authors.affiliation: Department of Neonatology, Hospital Clínico San Carlos-IdISSC, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Arruza
authors.affiliation: Health Research Institute Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: Maria Ruth
authors.initials: MR
authors.lastname: Pazos
authors.affiliation: Health Research Institute Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: Nagat
authors.initials: N
authors.lastname: Mohammed
authors.affiliation: Department of Pathology, Hospital Clínico San Carlos, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Escribano
authors.affiliation: Intensive Care Translational Research Group, Biocruces Health Research Institute, Bizkaia, Spain.
authors.forename: Hector
authors.initials: H
authors.lastname: Lafuente
authors.affiliation: Health Research Institute Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: Martín
authors.initials: M
authors.lastname: Santos
authors.affiliation: Intensive Care Translational Research Group, Biocruces Health Research Institute, Bizkaia, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Alvarez-Díaz
authors.affiliation: GW Research, Cambridge, UK.
authors.forename: William
authors.initials: W
authors.lastname: Hind
authors.affiliation: Department of Neonatology, Hospital Clínico San Carlos-IdISSC, Madrid, Spain.|Health Research Institute Puerta de Hierro Majadahonda, Madrid, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Martínez-Orgado
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid; D002185:Cannabidiol / Q000494:pharmacology*; D004195:Disease Models, Animal; D006439:Hemodynamics; D000860:Hypoxia / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology*; D007249:Inflammation / Q000188:drug therapy; D053583:Interleukin-1beta / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000503:physiopathology; D055370:Lung Injury / Q000188:drug therapy*; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D013552:Swine
chemical_list: D053583:Interleukin-1beta; D002185:Cannabidiol; D010100:Oxygen