title: Proteomic analysis of human plasma and peripheral blood mononuclear cells in Systemic Lupus Erythematosus patients.
abstract: Systemic Lupus Erythematosus (SLE) is a systemic autoimmune disease with a broad spectrum of clinical presentations and incompletely understood pathogenesis. This autoimmune disease is characterized by alterations in both the innate and adaptive immune system that lead to the loss of immunologic tolerance. In autoimmune diseases particularly in SLE, early diagnosis, flare or remission phases can be difficult to identify. Proteomics can help to find new therapeutic targets and it also could help to better understand the cellular mechanisms. The aim of this study was to observe the variations in plasma and Peripheral Blood Mononuclear Cells (PBMCs) proteome in order to increase our knowledge about pathogenesis and to find possible diagnostic markers and/or therapeutic targets for improving diagnosis and treatment. The comparative proteomic analyses showed that several proteins were differentially expressed in the PBMCs from SLE patients. Among these, PRDX2 may be used as candidate biomarker or target protein for further investigations. In plasma, we showed that plasma clusterin levels increased in SLE patients compared to healthy controls, but this increase is not statistically significant. These proteomic results provide suggestions for understanding the molecular mechanisms of SLE, as well as the physiological changes correlated with SLE disease.
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Pinna
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Pasella
authors.affiliation: Associazione "L'Isola dei Centenari", Sassari, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Deiana
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Baralla
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mannu
authors.affiliation: Internal Medicine 1, A.O.U. University of Sassari, Sassari, Italy.
authors.forename: Antonio Giovanni Emilio
authors.initials: AGE
authors.lastname: Masala
authors.affiliation: Internal Medicine 1, A.O.U. University of Sassari, Sassari, Italy.
authors.forename: Piera Veronica
authors.initials: PV
authors.lastname: Pileri
authors.affiliation: Associazione "L'Isola dei Centenari", Sassari, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Deiana
authors.affiliation: Surgical Pathology, A.O.U. University of Sassari, Sassari, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Scognamillo
authors.affiliation: Surgical Pathology, A.O.U. University of Sassari, Sassari, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Pala
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy; Associazione "L'Isola dei Centenari", Sassari, Italy. Electronic address: deiana@uniss.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Deiana
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054464:Peroxiredoxins / Q000097:blood / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D020543:Proteome; C086065:PRDX2 protein, human; D054464:Peroxiredoxins
keywords: Autoimmune diseases; PBMC; Proteomic; SLE; Two dimensional SDS PAGE