title: Skin inflammation induced by lupus serum was inhibited in IL-1R deficient mice.
abstract: Skin inflammation induced by lupus serum is a useful tool to investigate the pathogenesis of lupus skin injury. IL-1 is a proinflammatory cytokine, and its role in lupus skin lesion is still unclear. We determined the role of IL-1 in lupus skin injury by using gene deficient mice. We found that skin inflammation induced by lupus serum was significantly reduced in IL-1R deficient mice and caspase-1 deficient mice. IL-1R deficiency did not affect the expression of FcγRI (CD64), FcγRII (CD32) and MHC class II (CD74) induced by lupus serum. IL-1R deficiency reduced the lipid raft clustering, and decreased expression of MCP-1 and TNFα in monocytes. Keratinocyte proliferation induced by lupus serum was significantly decreased in TNFα deficient mice. Our findings indicate that IL-1 plays an important role in skin lesions of SLE. This study suggests that IL-1 is a therapeutic target in skin lesions of SLE.
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Xuanxuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Huicheng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Gongming
authors.initials: G
authors.lastname: Gao
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Guangqiong
authors.initials: G
authors.lastname: Xu
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Xibin
authors.initials: X
authors.lastname: Fei
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Fang
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China. Electronic address: gmdeng@njmu.edu.cn.
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Deng
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000276:immunology; D020170:Caspase 1 / Q000235:genetics; D049109:Cell Proliferation; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D015603:Keratinocytes / Q000502:physiology; D008180:Lupus Erythematosus, Systemic; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D017452:Receptors, IgG / Q000276:immunology; D017472:Receptors, Interleukin-1 / Q000235:genetics; D044967:Serum / Q000276:immunology*; D012867:Skin / Q000473:pathology; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D039481:CD11b Antigen; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D017452:Receptors, IgG; D017472:Receptors, Interleukin-1; D020170:Caspase 1
keywords: IL-1 receptor deficient mice; Lupus serum; Skin inflammation; Systemic lupus erythematosus (SLE)