title: Biodegradation of BTEX Aromatics by a Haloduric Microbial Consortium Enriched from a Sediment of Bohai Sea, China.
abstract: This study focused on a haloduric BTEX-degrading microbial consortium EC20 enriched from Bohai Sea sediment. EC20 degraded 87% of BTEX at 435 mg L-1initial concentration (benzene, toluene, ethylbenzene, and xylenes in equal proportions) in the presence of 3.4% NaCl. 16S rRNA gene-based PCR-DGGE profiles revealed that the dominant bacteria in EC20 were Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes at the phylum level, and Pseudomonas, Mesorhizobium, Achromobacter, Stenotrophomonas, and Halomonas at the genus level. PCR detection of genes coding the key enzymes which participated in BTEX degradation pathways showed that the enriched consortium EC20 contained TOL pathway and TOD pathway to initiate biodegradation of BTEX.
authors.affiliation: State Key Laboratory of AgroBiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Deng
authors.affiliation: State Key Laboratory of AgroBiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Fenglong
authors.initials: F
authors.lastname: Yang
authors.affiliation: State Key Laboratory of AgroBiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Deng
authors.affiliation: State Key Laboratory of AgroBiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Jinshui
authors.initials: J
authors.lastname: Yang
authors.affiliation: National Research Center for Geoanalysis, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.affiliation: State Key Laboratory of AgroBiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China. hlyuan@cau.edu.cn.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yuan
mesh_terms: D001554:Benzene / Q000302:isolation & purification / Q000378:metabolism; D001555:Benzene Derivatives / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D002681:China; D004785:Environmental Pollutants / Q000302:isolation & purification / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D006841:Hydrocarbons, Aromatic / Q000302:isolation & purification / Q000378:metabolism*; D059013:Microbial Consortia; D009792:Oceans and Seas; D014050:Toluene / Q000302:isolation & purification / Q000378:metabolism; D014992:Xylenes / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D004785:Environmental Pollutants; D006841:Hydrocarbons, Aromatic; D014992:Xylenes; D014050:Toluene; D001554:Benzene; C004912:ethylbenzene
keywords: BTEX; Bacterial consortium; Biodegradation; Metabolic pathway; PCR-DGGE