title: Prooxidant-Antioxidant Balance and Antioxidant Properties of Thuja orientalis L: A Potential Therapeutic Approach for Diabetes Mellitus.
abstract: Diabetes mellitus (DM) is a major health problem with an increasing global prevalence. It is usually associated with an imbalance between pro-oxidant mechanisms and antioxidant defenses, contributing to oxidative-stress, and this leads to an increased susceptibility to endothelial dysfunction, atherosclerosis, insulin-resistance and impaired-pancreatic β-cell function.
  
  
  
  We have assessed the Prooxidant-antioxidant balance (PAB) and anti-hemolytic effect of Thuja orientalis L using the PAB assay and the analysis of hematological markers.
  
  
  
  The antioxidant and anti-hemolytic activity of Thuja orientalis was evaluated using the PAB assay and the inhibition of RBC hemolysis using the hydrogen peroxide hemolysis test. The percentage of anti-hemolysis was calculated from the ratio of the measurements (A-B)/B×100.
  
  
  
  Our results showed that the antioxidant effect of Thuja orientalis L. was greater in water than in ethyl-acetate, ethanol and methanol extract, respectively. We also observed its anti-hemolytic effect, which was higher in water, than in ethyl-acetate, methanol and ethanol extract, respectively. In particular our data showed that the H2O2-induced RBC hemolysis was inhibited in a dose-dependent manner.
  
  
  
  we demonstrated the antioxidant and anti-hemolytic effect of Thuja orientalis L. extracts in human serum and RBC, showing its potential property of reducing free radicals supporting further investigations to assess its functional role in larger samples size and in vivo models, as a potential antioxidant agent.
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Daryoush H
authors.initials: DH
authors.lastname: Alamdari
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Malihe
authors.initials: M
authors.lastname: Aghasizadeh-Sharbaf
authors.affiliation: Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mohadjerani
authors.affiliation: Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, United Kingdom.
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Ferns
authors.affiliation: Metabolic syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Avan
mesh_terms: D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000494:pharmacology; D003920:Diabetes Mellitus / Q000188:drug therapy*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D016877:Oxidants / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D029783:Thuja / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016877:Oxidants; D010936:Plant Extracts; D001205:Ascorbic Acid
keywords: Antioxidant; Diabetes mellitus (DM); PAB assay; Thuja orientalis L; anti-hemolytic; therapeutic approaches.