title: Four Aromatic Sulfates with an Inhibitory Effect against HCV NS3 Helicase from the Crinoid Alloeocomatella polycladia.
abstract: Bioassay-guided separation of a lipophilic extract of the crinoidAlloeocomatella polycladia, inhibiting the activity of HCV NS3 helicase, yielded two groups of molecules: cholesterol sulfate and four new aromatic sulfates1-4. The structures of the aromatics were elucidated by spectroscopic analysis in addition to theoretical studies. The aromatic sulfates1-4showed moderate inhibition against NS3 helicase with IC50values of 71, 95, 7, and 5 μM, respectively.
authors.affiliation: Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan. hermawan_idam@yahoo.com.
authors.forename: Idam
authors.initials: I
authors.lastname: Hermawan
authors.affiliation: Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. atsushi.5961@ruri.waseda.jp.|Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. atsushi.5961@ruri.waseda.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Furuta
authors.affiliation: Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan. higashi@sci.u-ryukyu.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Higashi
authors.affiliation: General Education Center, Okinawa Prefectural University of Arts, 1-4 Shuri Tounokura, Naha, Okinawa 903-8602, Japan. fujitayo@okigei.ac.jp.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Fujita
authors.affiliation: Radioisotope Center, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. akimitsu@ric.u-tokyo.ac.jp.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Akimitsu
authors.affiliation: Department of Microbiology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi 409-3898, Japan. atsuyay@yamanashi.ac.jp.
authors.forename: Atsuya
authors.initials: A
authors.lastname: Yamashita
authors.affiliation: Department of Microbiology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi 409-3898, Japan. kmoriishi@yamanashi.ac.jp.
authors.forename: Kohji
authors.initials: K
authors.lastname: Moriishi
authors.affiliation: Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. stsuneda@waseda.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.affiliation: Environmental Measurement Research Institute, National Institute of Advanced Industrial Science and Technology, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan. h.tani@aist.go.jp.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tani
authors.affiliation: Department of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi-shi, Chiba 274-8510, Japan. captain.resonance@gmail.com.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nakakoshi
authors.affiliation: Institute of Medical Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. tsubuki@hoshi.ac.jp.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tsubuki
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. y.sekiguchi@aist.go.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sekiguchi
authors.affiliation: Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. noda-naohiro@aist.go.jp.|Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. noda-naohiro@aist.go.jp.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Noda
authors.affiliation: Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan. jtanaka@sci.u-ryukyu.ac.jp.
authors.forename: Junichi
authors.initials: J
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry*; D004447:Echinodermata / Q000737:chemistry*; D016174:Hepacivirus / Q000187:drug effects; D020365:RNA Helicases / Q000037:antagonists & inhibitors*; D013431:Sulfates / Q000494:pharmacology*; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors*
chemical_list: D000998:Antiviral Agents; D013431:Sulfates; D017361:Viral Nonstructural Proteins; D020365:RNA Helicases
keywords: NS3 helicase; aromatic sulfates; crinoid; hepatitis C virus (HCV)