title: Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation.
abstract: Lignin, one of the most abundant renewable feedstock, is used to develop a biocompatible hydrogel as anti-infective ointment. A hydrophilic polyoxazoline chain is grafted through ring opening polymerization, possess homogeneous spherical nanoparticles of 10-15 nm. The copolymer was covalently modified with triazole moiety to fortify the antimicrobial and antibiofilm activities. The hydrogel was capable of down regulating the expression level of IL-1β in LPS induced macrophage cells, and to cause significant reduction of iNOS production. It supported cellular anti-inflammatory activity which was confirmed with luciferase assay, western blot, and NF-κB analysis. This novel lignin-based hydrogel tested in-vivo has shown the abilities to prevent infection of burn wound, aid healing, and an anti-inflammatory dressing material. The hydrogel reported here provides a new material platform to introduce a cost-effective and efficient ointment option after undertaking further work to look at its use in the area of clinical practice.
authors.affiliation: Central Research Facility, Rubber Technology Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India.
authors.forename: Denial
authors.initials: D
authors.lastname: Mahata
authors.affiliation: Department of Neurological Sciences, Rush University Medical Centre, Chicago, IL, USA.
authors.forename: Malabendu
authors.initials: M
authors.lastname: Jana
authors.affiliation: Department of Neurological Sciences, Rush University Medical Centre, Chicago, IL, USA.
authors.forename: Arundhuti
authors.initials: A
authors.lastname: Jana
authors.affiliation: Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri-734 013, WB, India.
authors.forename: Nibendu
authors.initials: N
authors.lastname: Mondal
authors.affiliation: OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri-734 013, WB, India.
authors.forename: Tilak
authors.initials: T
authors.lastname: Saha
authors.affiliation: OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri-734 013, WB, India.
authors.forename: Subhajit
authors.initials: S
authors.lastname: Sen
authors.affiliation: Central Research Facility, Rubber Technology Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India.
authors.forename: Golok B
authors.initials: GB
authors.lastname: Nando
authors.affiliation: Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Chinmay K
authors.initials: CK
authors.lastname: Mukhopadhyay
authors.affiliation: OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri-734 013, WB, India.
authors.forename: Ranadhir
authors.initials: R
authors.lastname: Chakraborty
authors.affiliation: Central Research Facility, Rubber Technology Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India.
authors.forename: Santi M
authors.initials: SM
authors.lastname: Mandal
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008031:Lignin / Q000008:administration & dosage / Q000627:therapeutic use; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009824:Ointments / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014230:Triazoles / Q000008:administration & dosage / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D016328:NF-kappa B; D009824:Ointments; D014230:Triazoles; D008031:Lignin