title: Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts.
abstract: Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.
authors.affiliation: Sanquin Research, Dept. Hematopoiesis, Amsterdam, The Netherlands and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Marten
authors.initials: M
authors.lastname: Hansen
authors.affiliation: Sanquin Research, Dept. Hematopoiesis, Amsterdam, The Netherlands and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Eszter
authors.initials: E
authors.lastname: Varga
authors.affiliation: Sanquin Research, Dept. Hematopoiesis, Amsterdam, The Netherlands and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Wüst
authors.affiliation: Department of Laboratory Medicine, Laboratory of Hematology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands.
authors.forename: Nelleke
authors.initials: N
authors.lastname: Brouwer
authors.affiliation: Institut de recherches cliniques de Montréal (IRCM), Département de microbiologie, infectiologie et immunologie, Université de Montréal, Canada.
authors.forename: Hugues
authors.initials: H
authors.lastname: Beauchemin
authors.affiliation: Academic Medical Centre Amsterdam, Dept. Clinical genetics (cytogenetics laboratory), Amsterdam, The Netherlands.
authors.forename: Clemens
authors.initials: C
authors.lastname: Mellink
authors.affiliation: Academic Medical Centre Amsterdam, Dept. Clinical genetics (cytogenetics laboratory), Amsterdam, The Netherlands.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: van der Kevie-Kersemaekers
authors.affiliation: Institut de recherches cliniques de Montréal (IRCM), Département de microbiologie, infectiologie et immunologie, Université de Montréal, Canada; Division of Experimental Medicine, McGill University, Montréal, QC, Canada.
authors.forename: Tarik
authors.initials: T
authors.lastname: Möröy
authors.affiliation: Department of Laboratory Medicine, Laboratory of Hematology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands.
authors.forename: Bert
authors.initials: B
authors.lastname: van der Reijden
authors.affiliation: Sanquin Research, Dept. Hematopoiesis, Amsterdam, The Netherlands and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Marieke
authors.initials: M
authors.lastname: von Lindern
authors.affiliation: Sanquin Research, Dept. Hematopoiesis, Amsterdam, The Netherlands and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands. Electronic address: e.vandenakker@sanquin.nl.
authors.forename: Emile
authors.initials: E
authors.lastname: van den Akker
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002460:Cell Line; D065150:Cellular Reprogramming; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D059785:Karyotype; D016086:Lentivirus / Q000235:genetics; D008297:Male; D055016:Megakaryocyte Progenitor Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D040201:Platelet Membrane Glycoprotein IIb; D014157:Transcription Factors