title: Gene methylation as a powerful biomarker for detection and screening of non-small cell lung cancer in blood.
abstract: DNA methylation has been reported to become a potential powerful tool for cancer detection and diagnosis. However, the possibilities for the application of blood-based gene methylation as a biomarker for non-small cell lung cancer (NSCLC) detection and screening remain unclear. Hence, we performed this meta-analysis to evaluate the value of gene methylation detected in blood samples as a noninvasive biomarker in NSCLC. A total of 28 genes were analyzed from 37 case-control studies. In the genes with more than three studies, we found that the methylation of P16, RASSF1A, APC, RARβ, DAPK, CDH13, and MGMT was significantly associated with risks of NSCLC. The methylation statuses of P16, RASSF1A, APC, RARβ, DAPK, CDH13, and MGMT were not linked to age, gender, smoking behavior, and tumor stage and histology in NSCLC. Therefore, the use of the methylation status of P16, RASSF1A, APC, RARβ, DAPK, CDH13, and MGMT could become a promising and powerful biomarker for the detection and screening of NSCLC in blood in clinical settings. Further large-scale studies with large sample sizes are necessary to confirm our findings in the future.
authors.affiliation: Department of Thoracic Surgery, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: Bao-Hua
authors.initials: BH
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: Yan-Yu
authors.initials: YY
authors.lastname: Li
authors.affiliation: The Second Department of Oncology, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: Jin-Zhu
authors.initials: JZ
authors.lastname: Han
authors.affiliation: Department of Thoracic Surgery, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: Lian-Ya
authors.initials: LY
authors.lastname: Zhou
authors.affiliation: The Second Department of Oncology, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: Ying-Qian
authors.initials: YQ
authors.lastname: Lv
authors.affiliation: Department of Thoracic Surgery, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: He-Lin
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: The Second Department of Oncology, The Second Hospital of Heibei Medical University, Shijiazhuang 050000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
mesh_terms: D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics*; D016022:Case-Control Studies; D019175:DNA Methylation; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D016017:Odds Ratio; D017594:Publication Bias
chemical_list: D014408:Biomarkers, Tumor
keywords: NSCLC; biomarkers; blood; gene methylation