title: Biochemistry of malaria parasite infected red blood cells by X-ray microscopy.
abstract: Red blood cells infected by the malaria parasite Plasmodium falciparum are correlatively imaged by tomography using soft X-rays as well as by scanning hard nano-X-ray beam to obtain fluorescence maps of various elements such as S and Fe. In this way one can deduce the amount of Fe bound either in hemoglobin or in hemozoin crystals in the digestive vacuole of the malaria parasite as well as determine the hemoglobin concentrations in the cytosols of the red blood cell and of the parasite. Fluorescence map of K shows that in the parasite's schizont stage the K concentration in the red blood cell cytosol is diminished by a factor of seven relative to a pristine red blood cell but the total amount of K in the infected red blood cell is the same as in the pristine red blood cell.
authors.affiliation: Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen Ø, Denmark. sergey.kapishnikov@nbi.ku.dk.
authors.forename: S
authors.initials: S
authors.lastname: Kapishnikov
authors.affiliation: Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Leiserowitz
authors.affiliation: European Synchrotron Radiation Facility (ESRF), 71 avenue des Martyrs, 38000, Grenoble, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.affiliation: European Synchrotron Radiation Facility (ESRF), 71 avenue des Martyrs, 38000, Grenoble, France.
authors.forename: P
authors.identifier: http://orcid.org/0000-0002-4129-9091
authors.initials: P
authors.lastname: Cloetens
authors.affiliation: ALBA Synchrotron Light Source, MISTRAL Beamline-Experiments Division, 08290, Cerdanyola del Valles, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Pereiro
authors.affiliation: Molecular Parasitology, Institute of Biology, Humboldt University, Philippstr. 13, 10115, Berlin, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Awamu Ndonglack
authors.affiliation: Molecular Parasitology, Institute of Biology, Humboldt University, Philippstr. 13, 10115, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Matuschewski
authors.affiliation: Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen Ø, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Als-Nielsen
mesh_terms: D004577:Electron Probe Microanalysis / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism / Q000469:parasitology; D006420:Hemeproteins / Q000032:analysis / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000032:analysis; D016778:Malaria, Falciparum / Q000097:blood*; D010963:Plasmodium falciparum / Q000472:pathogenicity; D011188:Potassium / Q000032:analysis; D013455:Sulfur / Q000032:analysis
chemical_list: D006420:Hemeproteins; D006454:Hemoglobins; C020753:hemozoin; D013455:Sulfur; D007501:Iron; D011188:Potassium