title: Dietary Allium hookeri reduces inflammatory response and increases expression of intestinal tight junction proteins in LPS-induced young broiler chicken.
abstract: We undertook a study to assess the effects of Allium hookeri (AH)
  root and fermented root on inflammation and intestinal integrity of
  lipopolysaccharide (LPS)-challenged broiler chickens. Birds were assigned
  to six groups (n = 25 birds/treatment) and fed with basal diets
  (CON) or basal diets supplemented with AH root or fermented root at
  two concentrations (1 or 5%). At 7 d of age, five groups (n= 125) in
  each dietary treatment were injected with LPS (1 mg/kg body weight),
  and the remaining 25 birds were injected with sterile phosphate-buffered
  saline (PBS) as a negative control. LPS challenge significantly
  reduced average body weight gain at 24 h post-injection compared with
  PBS control. Fermented root supplementation increased average body
  weight gain by 1% compared with the LPS-challenged control. Serum α-
  1-AGP levels, interleukin (IL)-1β, IL-8, tumor necrosis factor superfamily
  member 15 (TNFSF15), and LPS-induced tumor necrosis factor-α
  factor (LITAF) transcript levels were significantly higher in the small
  intestine in LPS-injected chickens. However α-1-AGP levels were reduced
  by AH root or fermented root (1 and 5%) supplementation and
  IL-1β, IL-8, and LITAF were also down-regulated by root and fermented
  root (1 and 5%) supplementation. The reduced expression of tight
  junction proteins (junctional adhesion molecule 2 (JAM2) and occludin)
  and intestinal mucin 2 (MUC2) by LPS challenge was reversed
  by root or fermented root (1 and 5%) supplementation. These findings
  demonstrate that dietary AH root and fermented root influence antiinflammatory
  activity and tight junction protein expression in LPS-induced
  chickens.
authors.affiliation: Animal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, USA.
authors.forename: Youngsub
authors.initials: Y
authors.lastname: Lee
authors.affiliation: National Institute of Agricultural Sciences, Rural Department Administration, 166, Nongsaengmyeong-ro, Isoe-Myeon, Wanju-Gun, Jeollabuk-do, South Korea.
authors.forename: Sung-Hyen
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Animal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, USA.
authors.forename: Ujvala Deepthi
authors.initials: UD
authors.lastname: Gadde
authors.affiliation: Animal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, USA.
authors.forename: Sung-Taek
authors.initials: ST
authors.lastname: Oh
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Animal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, USA. Electronic address: hyun.lillehoj@ars.usda.gov.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
mesh_terms: D000490:Allium / Q000737:chemistry*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D007422:Intestines / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D011201:Poultry Diseases / Q000188:drug therapy / Q000276:immunology; D062725:Tight Junction Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D062725:Tight Junction Proteins