title: Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding.
abstract: Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women.Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation.Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy; CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, Italy.
authors.forename: Carmela
authors.identifier: 0000-0002-9540-3405
authors.initials: C
authors.lastname: Nardelli
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy; CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Iaffaldano
authors.affiliation: Dipartimento di Medicina e Chirurgia, Università di Salerno, Via Giovanni Paolo II 132, Fisciano, 84084 Salerno, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Pilone
authors.affiliation: IRCCS SDN-Istituto di Ricerca Diagnostica e Nucleare, Via Gianturco 113, 80100 Naples, Italy.
authors.forename: Giuseppe
authors.identifier: 0000-0002-4436-846X
authors.initials: G
authors.lastname: Labruna
authors.affiliation: CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, Italy.
authors.forename: Maddalena
authors.identifier: 0000-0003-4824-5886
authors.initials: M
authors.lastname: Ferrigno
authors.affiliation: Dipartimento di Scienze Biomediche Avanzate, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Nicola
authors.identifier: 0000-0002-9837-5809
authors.initials: N
authors.lastname: Carlomagno
authors.affiliation: Dipartimento di Scienze Biomediche Avanzate, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Concetta Anna
authors.initials: CA
authors.lastname: Dodaro
authors.affiliation: Dipartimento di Medicina Clinica e Chirurgia, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Forestieri
authors.affiliation: IRCCS SDN-Istituto di Ricerca Diagnostica e Nucleare, Via Gianturco 113, 80100 Naples, Italy; Dipartimento Scienze Motorie e del Benessere, Università di Napoli Parthenope, Via Amm. F. Acton 38, 80133 Naples, Italy.
authors.forename: Pasqualina
authors.identifier: 0000-0003-0755-6143
authors.initials: P
authors.lastname: Buono
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy; CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, Italy.
authors.forename: Francesco
authors.identifier: 0000-0002-2346-3564
authors.initials: F
authors.lastname: Salvatore
authors.affiliation: CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Naples, Italy.
authors.forename: Lucia
authors.identifier: 0000-0002-1550-8216
authors.initials: L
authors.lastname: Sacchetti
mesh_terms: D000199:Actins / Q000032:analysis; D017667:Adipocytes / Q000166:cytology; D052242:Adiponectin / Q000097:blood; D000328:Adult; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D005743:Gastrectomy; D006801:Humans; D010535:Laparoscopy; D020738:Leptin / Q000097:blood; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D009767:Obesity, Morbid / Q000601:surgery*; D047493:PPAR alpha / Q000032:analysis; D050151:Subcutaneous Fat / Q000737:chemistry*; D016387:Women's Health
chemical_list: D000199:Actins; D052242:Adiponectin; D020738:Leptin; D035683:MicroRNAs; D047493:PPAR alpha