title: Online Flowing Colloidosomes for Sequential Multi-analyte High-Throughput SERS Analysis.
abstract: 3D plasmonic colloidosomes are superior SERS sensors owing to their high sensitivity and excellent tolerance to laser misalignment. Herein, we incorporate plasmonic colloidosomes in a microfluidic channel for online SERS detection. Our method resolves the poor signal reproducibility and inter-sample contamination in the existing online SERS platforms. Our flow system offers rapid and continuous online detection of 20 samples in less than 5 min with excellent signal reproducibility. The isolated colloidosomes prevent cross-sample and channel contamination, allowing accurate quantification of samples over a concentration range of five orders of magnitude. Our system demonstrates high-resolution multiplex detection with fully preserved signal and Raman features of individual analytes in a mixture. High-throughput multi-assay analysis is performed, which highlights that our system is capable of rapid identification and quantification of a sequence of samples containing various analytes and concentrations.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Gia Chuong
authors.initials: GC
authors.lastname: Phan-Quang
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Elizabeth Hui Zi
authors.initials: EHZ
authors.lastname: Wee
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Fengling
authors.initials: F
authors.lastname: Yang
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.|Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, Singapore, 138634, Singapore.
authors.forename: Hiang Kwee
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03, Singapore, 138634, Singapore.
authors.forename: In Yee
authors.initials: IY
authors.lastname: Phang
authors.affiliation: Departamento de Química Física e Inorgánica and Emas, Universitat Rovira i Virgili, Carrer de Marcellí Domingo s/n, 43007, Tarragona, Spain.|ICREA, Passeig Lluís Companys 23, 08010, Barcelona, Spain.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Feng
authors.affiliation: Departamento de Química Física e Inorgánica and Emas, Universitat Rovira i Virgili, Carrer de Marcellí Domingo s/n, 43007, Tarragona, Spain.|ICREA, Passeig Lluís Companys 23, 08010, Barcelona, Spain.
authors.forename: Ramón A
authors.initials: RA
authors.lastname: Alvarez-Puebla
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Xing Yi
authors.identifier: 0000-0001-5495-6428
authors.initials: XY
authors.lastname: Ling
keywords: SERS; colloidosomes; microfluidics; multiplex assays; optical detection