title: Effect on intensity of treadmill running on learning, memory and expressions of cell cycle-related proteins in rats with cerebral ischemia.
abstract: OBJECTIVE
  We discussed the intensity of treadmill running on learning, memory and expression of cell cycle-related proteins in rats with cerebral ischemia.
  
  
  METHODS
  Eighty healthy male SD rats were randomly divided into normal group, model group, intensity I group and intensity II group, with 20 rats in each group. The four-vessel occlusion method of Pulsinelli (4-VO) was used to induce global cerebral ischemia. Brain neuronal morphology was observed by hematoxylin-eosin (HE) staining at 3h, 6h, 24h and 48h after modeling, respectively. Hippocampal expressions of cyclin A and cyclin E were detected by immunohistochemistry. At 48h after modeling, the learning and memory performance of rats was tested by water maze experiment.
  
  
  RESULTS
  Compared with the normal group, the other three groups had a significant reduction in surviving neurons, prolonging of escape latency and decreased number of passes over the former position of the platform (P<0.05). The number of surviving neurons and the number of passes over the former position of the platform were obviously lower in the model group than in intensity I group (P<0.05), but significantly higher compared with intensity II group (P<0.05). Escape latency of the model group was obviously prolonged as compared with intensity I group (P<0.05), but much shorter than that of intensity II group (P<0.05). Compared with the normal group, the expressions of cyclin A and cyclin E were significantly upregulated at different time points after modeling (P<0.05). The expression of the model group was higher than that of intensity I group, but lower than that of intensity II group (P<0.05).
  
  
  CONCLUSIONS
  Moderate intensity of treadmill running can help protect brain neurons and improve learning and memory performance of rats with global cerebral ischemia. But high intensity of treadmill running has a negative impact, possibly through the regulation of cell cycle-related proteins in ischemia/reperfusion injury.
authors.affiliation: Nursing and Rehabilitation College, North China University of Science and Technology, 063000, China.
authors.forename: Ya-Ning
authors.initials: YN
authors.lastname: Zhao
authors.affiliation: The Neurosurgery of Affiliated Hospital, North China University of Science and Technology, 063000, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Li
authors.affiliation: Nursing and Rehabilitation College, North China University of Science and Technology, 063000, China.
authors.forename: Chang-Xiang
authors.initials: CX
authors.lastname: Chen
authors.affiliation: Nursing and Rehabilitation College, North China University of Science and Technology, 063000, China.
authors.forename: Shu-Xing
authors.initials: SX
authors.lastname: Li
authors.affiliation: The Neurosurgery of Affiliated Hospital, North China University of Science and Technology, 063000, China.
authors.forename: Cheng-Jing
authors.initials: CJ
authors.lastname: Xue
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002470:Cell Survival; D019925:Cyclin A / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D008297:Male; D018782:Maze Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012420:Running / Q000502:physiology*; D013997:Time Factors
chemical_list: D018797:Cell Cycle Proteins; D019925:Cyclin A; D019927:Cyclin E
keywords: cerebral ischemia; cyclin A; cyclin E; learning and memory; treadmill running