title: [Quantity of Treg cells and Th17 cells in spleen of primary immune thrombocytopenic purpura patients].
abstract: Objective:To observe the quantity of Treg cells and Th17 cells in spleen of adult primary immune thrombocytopenic purpura (ITP) patients.Methods:43 ITP cases with splenectomy treatment were enrolled from December 2008 to June 2016 at Union Hospital of Fujian Medical University, including 20 males and 23 females with a median age of 36 (18-76) years. The controls were thirty patients who underwent splenectomy because of pancreatic diseases or splenic impairment, including 21 males and 9 females with a median age of 47 (21-69) years. The quantity and ratio of Treg cells and Th17 cells were examined by immunohistochemistry between ITP patients and controls.Results:①The quantity of Treg cells in ITP were less than controls[ (11.3±4.7) /mm(2)vs(59.0±15.0) /mm(2),t=-22.894,P<0.001], but Th17 cells were more than controls[ (235.2±69.4) /mm(2)vs(181.1±23.7) /mm(2),t=13.768,P<0.001]. So the ratio of Treg/Th17 in ITP was lower than controls (0.048±0.027vs0.328±0.086,t=19.522,P<0.001) . ② The quantity of Treg cells in cases without response after splenectomy were less than cases with response[ (9.5±5.0) /mm(2)vs(11.6±4.7) /mm(2),t=2.723,P=0.010], and there is no statistical differences between the two groups about the quantity of Th17 cells and the ratio of Treg/Th17 cells[ (232.3±80.8) /mm(2)vs(239.6±66.9) /mm(2),t=1.108,P=0.277; 0.040±0.024vs0.053±0.027,t=0.540,P=0.592].Conclusions:There is a significant difference about the quantity of Treg cells and Th17 cells in spleen between ITP patients and healthy controls, and they are relevant to the response after splenectomy.
authors.affiliation: Department of Genery Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yang
authors.forename: F Q
authors.initials: FQ
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Huang
authors.forename: D Y
authors.initials: DY
authors.lastname: Zeng
authors.forename: H G
authors.initials: HG
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic; D013154:Spleen; D013156:Splenectomy; D050378:T-Lymphocytes, Regulatory; D058504:Th17 Cells; D055815:Young Adult
keywords: Splenectomy; T-Lymphocytes, regulatory; Th17 cells; Thrombocytopenia