title: The integrative metabolomic-transcriptomic landscape of glioblastome multiforme.
abstract: The purpose of this study was to map the landscape of metabolic-transcriptional alterations in glioblastoma multiforme. Omic-datasets were acquired by metabolic profiling (1D-NMR spectroscopy n=33 Patient) and transcriptomic profiling (n=48 Patients). Both datasets were analyzed by integrative network modeling. The computed model concluded in four different metabolic-transcriptomic signatures containing: oligodendrocytic differentiation, cell-cycle functions, immune response and hypoxia. These clusters were found being distinguished by individual metabolism and distinct transcriptional programs. The study highlighted the association between metabolism and hallmarks of oncogenic signaling such as cell-cycle alterations, immune escape mechanism and other cancer pathway alterations. In conclusion, this study showed the strong influence of metabolic alterations in the wide scope of oncogenic transcriptional alterations.
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Dieter Henrik
authors.initials: DH
authors.lastname: Heiland
authors.affiliation: Institute of Physical Chemistry, Faculty of Chemistry and Pharmacy, University of Freiburg, Freiburg, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: Wörner
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Gerrit Haaker
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Delev
authors.affiliation: Institute of Physical Chemistry, Faculty of Chemistry and Pharmacy, University of Freiburg, Freiburg, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Pompe
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Bianca
authors.initials: B
authors.lastname: Mercas
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Pamela
authors.initials: P
authors.lastname: Franco
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Gäbelein
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Heynckes
authors.affiliation: Department of Hematology, Oncology and Stem Cell Transplantation, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Pfeifer
authors.affiliation: Institute of Physical Chemistry, Faculty of Chemistry and Pharmacy, University of Freiburg, Freiburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Weber
authors.affiliation: Department of Neuroradiology, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Mader
authors.affiliation: Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.|Faculty of Medicine, University of Freiburg, Freiburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Schnell
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism*; D016000:Cluster Analysis; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods; D053263:Gene Regulatory Networks; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D059467:Transcriptome; D057188:Workflow
keywords: WGCNA; glioblastoma multiforme; metabolomics; network analysis; transcriptomics