title: Naringin ameliorates diabetic nephropathy by inhibiting NADPH oxidase 4.
abstract: Naringin, a naturally flavanone glycoside, has been previously demonstrated to alleviate diabetic kidney disease by inhibiting oxidative stress and inflammatory reaction. However, the underlying mechanism of naringin in diabetic nephropathy (DN) has not been fully elucidated. Here, the beneficial effect of naringin on DN in streptozotocin (STZ)-induced DN rats and high glucose (HG)-induced podocytes and its underlying mechanism were elaborated. The result revealed that naringin alleviated STZ-induced renal dysfunction and injury in DN rats, relieved STZ-induced oxidative stress in vivo and inhibited HG-induced apoptosis and reactive oxygen species level i20n vitro. More importantly, naringin inhibited NOX4 expression at mRNA and protein levels in STZ-induced DN rats and HG-induced podocytes. Loss of function indicated that NADPH oxidases 4 (NOX4) down-regulation suppressed apoptosis and reactive oxygen species level in HG-treated podocytes. Take together, this study demonstrated that naringin ameliorates diabetic nephropathy by inhibiting NOX4, contributing to a better understanding of the progression of DN.
authors.affiliation: Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng 475000, China. Electronic address: junweizjw@yeah.net.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Huicong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D044950:Flavanones / Q000494:pharmacology* / Q000627:therapeutic use; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008297:Male; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D050199:Podocytes / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D044950:Flavanones; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; C498911:Nox4 protein, rat; C005274:naringin
keywords: Diabetic nephropathy; NOX4; Naringin; Oxidative stress; Reactive oxygen species