title: STZ causes depletion of immune cells in sciatic nerve and dorsal root ganglion in experimental diabetes.
abstract: Streptozotocin (STZ) treatment, a common model for inducing diabetes in rodent models, induces thermal hyperalgesia and neuronal toxicity independently of hyperglycemia by oxidizing and activating TRPA1 and TRPV1. Following treatment with STZ, CD45+immune cells were found to be depleted in sciatic nerve (SN) and DRG in mice, prior to hyperglycemia. Macrophages were also lost in DRG and NFκB-p65-activation was increased in SN macrophages. Immune cells were significantly reduced in both SN and DRG up to three weeks, post-treatment. Loss of PNS-resident macrophages in response to STZ-mediated toxicity may affect the regenerative capacity of the nerve in response to further injury caused by diabetes.
authors.affiliation: Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, INF 410, Heidelberg, Germany.
authors.forename: Asa S
authors.initials: AS
authors.lastname: Hidmark
authors.affiliation: Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, INF 410, Heidelberg, Germany; Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, Neuherberg, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Nawroth
authors.affiliation: Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, INF 410, Heidelberg, Germany; German Center for Diabetes Research (DZD), Ingolstädter Landstraße 1, 85764 Neuherberg, Germany. Electronic address: Thomas.Fleming@med.uni-heidelberg.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Fleming
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D015703:Antigens, CD / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005727:Ganglia, Spinal / Q000187:drug effects / Q000473:pathology*; D006930:Hyperalgesia / Q000209:etiology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007962:Leukocytes / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology*; D013311:Streptozocin / Q000633:toxicity; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D015703:Antigens, CD; D016207:Cytokines; D018799:Intercellular Adhesion Molecule-1; D013311:Streptozocin
keywords: Diabetic neuropathy; Experimental diabetes; Macrophage; Neurotoxicity