title: Structural analysis of SgvP involved in carbon-sulfur bond formation during griseoviridin biosynthesis.
abstract: Griseoviridin (GV) is a broad-spectrum antibiotic with antibacterial and antifungal activity. In the GV biosynthetic pathway, SgvP catalyzes formation of the carbon-sulfur bond in GV. Herein, we report the recombinant expression and characterization of SgvP from Streptomyces griseoviridis NRRL2427. We also present the 2.6 Å crystal structure of SgvP, which is the first structure of a cytochrome P450 involved in carbon-sulfur bond formation in GV. Structural analysis indicates that Pro237 in the I-helix of SgvP may play a critical role in dioxygen binding and proton transfer during the catalytic cycle. Of the three channels we observed in SgvP, channel 3 may be essential for substrate ingress and egress from the active site, while channels 1 and 2 may be the solvent and water pathway, respectively.
  
  
  
  Coordinate and structure factor were deposited in the Protein Data Bank database under the accession number 4MM0.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.|Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Hubei, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Dalian Institute for Food Control, Dalian Food and Drug Administration, Liaoning, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Dainan Senior High School, Taizhou, Jiangsu, China.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Hubei, China.
authors.forename: Huaidong
authors.identifier: 0000-0001-6572-4737
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011392:Proline / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013455:Sulfur / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; C010583:griseoviridin; D013455:Sulfur; D002244:Carbon; D003577:Cytochrome P-450 Enzyme System; D011392:Proline; D010100:Oxygen
keywords: carbon-sulfur bond; crystal structure; griseoviridin