title: Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity.
abstract: Hyperoxaluria and oxidative stress are risk factors in calcium oxalate (CaOx) stone formation. Supplement with antioxidant could be effective in prevention of recurrent stone formation. The present study aims to evaluate the protective effects of vitamin E and vitamin C in hyperoxaluric rat. The experiment was performed in rats for 21 days. Rats were divided into 5 groups as follows: control (group 1, n=8), hyperoxaluric rats (group 2, n=8), hyperoxaluric rats with vitamin E supplement (group 3, n=7), hyperoxaluric rats with vitamin C supplement (group 4, n=7) and hyperoxaluric rats with vitamin E and C supplement (group 5, n=7). Hyperoxaluria was induced by feeding hydroxyl L-proline (HLP) 2% w/v dissolved in drinking water. Intraperitoneal 200 mg/kg of vitamin E was given in groups 3 and 5 on days 1, 6, 11 and 16, while 500 mg of vitamin C was injected intravenously in groups 4 and 5 on days 1 and 11. Renal functions and oxidative status were measured. The urinary oxalate excretion was increased in HLP supplement rats, while glomerular filtration rate, proximal water and sodium reabsorption were significantly lower in group 2 compared with a control (P<0.05). Giving antioxidants significantly lower urinary calcium oxalate crystals (P<0.05). Hyperoxaluric rats had higher plasma malondialdehyde (PMDA) and lower urinary total antioxidant status (UTAS), which were alleviated by vitamin E and/or vitamin C supplement. In conclusion, giving combination of vitamin E and vitamin C exerts a protective role against HLP-induced oxalate nephropathy.
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Orapun
authors.initials: O
authors.lastname: Jaturakan
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Thasinas
authors.initials: T
authors.lastname: Dissayabutra
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Narongsak
authors.initials: N
authors.lastname: Chaiyabutr
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Anusak
authors.initials: A
authors.lastname: Kijtawornrat
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Piyaratana
authors.initials: P
authors.lastname: Tosukhowong
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Anudep
authors.initials: A
authors.lastname: Rungsipipat
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Thumnoon
authors.initials: T
authors.lastname: Nhujak
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Chollada
authors.initials: C
authors.lastname: Buranakarl
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D001835:Body Weight; D002951:Citrates / Q000652:urine; D004326:Drinking; D004359:Drug Therapy, Combination; D004435:Eating; D004573:Electrolytes / Q000378:metabolism; D006439:Hemodynamics; D006959:Hyperoxaluria / Q000188:drug therapy* / Q000473:pathology; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007669:Kidney Calculi / Q000517:prevention & control; D007678:Kidney Glomerulus / Q000187:drug effects / Q000502:physiology; D008297:Male; D010070:Oxalates / Q000652:urine; D020011:Protective Agents / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D002951:Citrates; D004573:Electrolytes; D010070:Oxalates; D020011:Protective Agents; D014810:Vitamin E; D001205:Ascorbic Acid
keywords: glomerular filtration rate; hyperoxaluria; oxidative stress; vitamin C; vitamin E