title: Evaluation of gelatin-hyaluronic acid composite hydrogels for accelerating wound healing.
abstract: Excellent wound dressings maintain a warm and moist environment, thus accelerating wound healing. In this study, we cross-linked gelatin and hyaluronic acid with ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride in different ratios (gelatin/hyaluronic acid = 8:2, gelatin/hyaluronic acid = 5:5, gelatin/hyaluronic acid = 2:8), and explored the effects and mechanisms of gelatinhyaluronic acid hydrogels on wound healing. This was done by examining dressing properties, such as fluid uptake ability, water vapor transmission rate, and the rate of water evaporation. We further verified biological function by using in vitro and in vivo wound models. The hydrogels display appropriate fluid uptake ability and good water vapor transmission rate and rate of water evaporation all of which can provide an adequate moisture environment for wound healing. Cell cytotoxicity and proliferation tests show that the hydrogels have no cytotoxicity, furthermore, gelatin/hyaluronic acid = 8:2 hydrogels have the potential to promote cell proliferation. Animal wound models demonstrate that the hydrogels can effectively promote wound healing in vivo, in particular, the gelatin/hyaluronic acid = 8:2 group which promoted the most rapid healing. Accordingly, gelatin-hyaluronic acid dressings, especially the gelatin/hyaluronic acid = 8:2 hydrogels, have a promising outlook for clinical applications in wound healing.
authors.affiliation: 1 Department of orthopedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Song
authors.initials: S
authors.lastname: Wu
authors.affiliation: 2 Xiangya medical school, Central South University, Changsha, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Deng
authors.affiliation: 1 Department of orthopedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Hanson
authors.initials: H
authors.lastname: Hsia
authors.affiliation: 2 Xiangya medical school, Central South University, Changsha, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: 2 Xiangya medical school, Central South University, Changsha, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.affiliation: 2 Xiangya medical school, Central South University, Changsha, China.
authors.forename: Qiangru
authors.initials: Q
authors.lastname: Huang
authors.affiliation: 1 Department of orthopedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Peng
authors.affiliation: 3 Key Laboratory of Education Ministry of Theoretical Chemistry and Molecular Simulation, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: 4 Department of Burns and Plastic Surgery of the 3rd Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry*; D002234:Carbodiimides / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D014945:Wound Healing
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D001672:Biocompatible Materials; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D020100:Hydrogels; D005780:Gelatin; D006820:Hyaluronic Acid
keywords: Gelatin; hyaluronic acid; hydrogel; moist environment; wound healing