title: Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio.
abstract: Most bacteria can swim by rotating the flagellum. The basal body of the flagellum is an essential part for this motor function. Recent comprehensive analysis of the flagellar basal body structures across bacteria by cryo-electron tomography has revealed that they all share core structures, the rod, and rings: the C ring, M ring, S ring, L ring, and P ring. Furthermore, it also has uncovered that in some bacteria, there are extra ring structures in the periplasmic space and outer-membrane. Here, we describe a protocol to isolate the basal body of the flagellar basal body from a marine bacterium, Vibrio alginolyticus, for structural analysis of additional ring structures, the T ring and H ring.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan. onoue.yasuhiro@h.mbox.nagoya-u.ac.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Onoue
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Homma
mesh_terms: D064652:Basal Bodies / Q000378:metabolism; D055032:Electron Microscope Tomography / Q000379:methods; D005407:Flagella / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D044149:Vibrio alginolyticus / Q000378:metabolism*
chemical_list: D020409:Molecular Motor Proteins; D012964:Sodium
keywords: Bacterial flagellum; Basal body; FlgT; H ring; MotX; MotY; T ring; Vibrio