title: Pathogenesis of coxsackievirus B2 in mice: characterization of clinical isolates of the coxsackievirus B2 from patients with myocarditis and aseptic meningitis in Korea.
abstract: Group B coxsackieviruses (CVBs) are a group of common human pathogens producing various clinical symptoms. Although the virology of CVB is well known, there is limited information on viral pathogenesis and the relationship between clinical symptoms and viral phenotype, particularly for CVB type 2 (CVB2). In 2004 in Korea, two CVB2 strains were isolated: CB2/04/279 from stool of an acute myocarditis patient with heart failure and CB2/04/243 from an aseptic meningitis patient. In this study, a high degree of homology was observed between the CB2/04/279 and CB2/04/243 full genome sequences. The two Korean CVB2 isolates had 93.1% homology compared to 82.1%-82.5% nucleotide sequence identity with the cardiovirulence-associated reference CVB strainOhio-1(CVB/O). CVB2-induced pathogenesis was analyzed, focusing on virus-induced pathology of various tissues in 4-week-old BALB/c inbred male mice. Myocarditis developed and extensive pancreatic inflammation was observed in all mice infected with CB2/04/279 or CVB/O, but not in animals infected with CB2/04/243. This is the first report of the full-genomic sequence and pathogenesis of the CVB2 strain isolated from an acute myocarditis patient in Korea.
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.|Vaccines Division, National Institute of Food & Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju 28159, Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Hong
authors.affiliation: Division of Vaccine Research, Center for Infectious Disease, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju 28159, Korea.
authors.forename: Bunghak
authors.initials: B
authors.lastname: Kang
authors.affiliation: Division of Vaccine Research, Center for Infectious Disease, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju 28159, Korea.
authors.forename: Sanggu
authors.initials: S
authors.lastname: Yeo
authors.affiliation: Division of Vaccine Research, Center for Infectious Disease, National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju 28159, Korea.
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jee
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D029822:Enterovirus B, Human / Q000235:genetics / Q000502:physiology*; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000473:pathology* / Q000821:virology; D010195:Pancreatitis / Q000473:pathology* / Q000821:virology
keywords: cardiovirulence; coxsackievirus B2; myocarditis; pathogenesis