title: Using a multi-biomarker approach to assess the effects of pollution on sand flathead (Platycephalus bassensis) from Port Phillip Bay, Victoria, Australia.
abstract: Hepatic gene expression and liver histology were examined in sand flathead (Platycephalus bassensis) from six locations in Port Phillip Bay, Victoria, Australia. Four sets of genes including thyroid-related genes (D1, D2, TTR, TRα and TRβ), metal metabolism-related genes (MT, MTF1, TF, Ferritin and FPN1), apoptosis-related genes (Diablo/SMAC1, Diablo/SMAC2 and CYP1A) and an endoplasmic reticulum stress biomarker gene (GRP78) were examined in female flathead using qRT-PCR. TRβ and Diablo/SMAC1 gene expression was significantly up-regulated in fish from all polluted sites compared to those from a reference site. The transcripts of TRα and FPN1 were significantly higher in flathead from Corio Bay, while the hepatic mRNA of TTR and GRP78 were significantly lower in those fish. Positive correlations were observed between Diablo/SMAC1 and CYP1A, D2 and TRβ, TRα and TRβ. This study demonstrates that application of pathway-based biomarker genes and histopathology can provide comprehensive information on the impact of environmental pollutants on fish.
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Locked Bag 1370, Launceston, Tasmania 7250, Australia.
authors.forename: Dingkun
authors.initials: D
authors.lastname: Fu
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Locked Bag 1370, Launceston, Tasmania 7250, Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bridle
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Locked Bag 1370, Launceston, Tasmania 7250, Australia.
authors.forename: Melanie
authors.initials: M
authors.lastname: Leef
authors.affiliation: Department of Environment & Agriculture, Curtin University, Bentley 6102, Western Australia, Australia.
authors.forename: Marthe Monique
authors.initials: MM
authors.lastname: Gagnon
authors.affiliation: Centre for Aquatic Pollution Identification and Management (CAPIM), The University of Melbourne, Parkville 3010, Victoria, Australia.
authors.forename: Kathryn L
authors.initials: KL
authors.lastname: Hassell
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Locked Bag 1370, Launceston, Tasmania 7250, Australia. Electronic address: B.Nowak@utas.edu.au.
authors.forename: Barbara F
authors.initials: BF
authors.lastname: Nowak
mesh_terms: D000818:Animals; D061006:Bays; D015415:Biomarkers; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005819:Genetic Markers; D014739:Victoria; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D014874:Water Pollutants, Chemical
keywords: Biomarkers; Gene expression; Histology; Port Phillip Bay; Sand flathead