title: Protective Effect of Juglans regia L. Walnut Extract Against Oxidative DNA Damage.
abstract: Walnuts (Juglans regia L.) are relevant components of the Mediterranean diet providing important macronutrients, micronutrients and other bioactive constituents including unsaturated fatty acids, proteins, fiber, vitamins, minerals, phytosterols and polyphenols. Although the walnut beneficial effects in human health are widely recognized by a lot of epidemiologic studies very little is known regarding its effect on damaged DNA. The aim of the present study was to investigate the effect of Juglans regia L. ethanolic extract from kernel on the induction of DNA strand breaks by thiol/Fe3+/O2mixed function oxidase, tert-butyl hydroperoxide or UVC radiations in acellular and cellular models. Plasmid DNA cleavage and fast Halo assay were used to monitor oxidative damage to DNA. Both approaches showed protection of oxidatively injured DNA. These results agree with a lot of scientific proofs which recommend walnut as dietary adjunct in health promotion and prevention as well as in treatment of lifestyle-related oxidative diseases.
authors.affiliation: Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via A. Saffi 2, I-61029, Urbino, PU, Italy.|Department for Life Quality Studies, Alma Mater Studiorum-University of Bologna, Corso d'Augusto 237, I 47921, Rimini, RN, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Calcabrini
authors.affiliation: Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via A. Saffi 2, I-61029, Urbino, PU, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: De Bellis
authors.affiliation: Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via A. Saffi 2, I-61029, Urbino, PU, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Mancini
authors.affiliation: Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via A. Saffi 2, I-61029, Urbino, PU, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Cucchiarini
authors.affiliation: Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via A. Saffi 2, I-61029, Urbino, PU, Italy.
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
authors.affiliation: Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via A. Saffi 2, I-61029, Urbino, PU, Italy. lucia.potenza@uniurb.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
mesh_terms: D002460:Cell Line; D053960:DNA Breaks / Q000187:drug effects / Q000528:radiation effects; D053837:DNA Cleavage / Q000187:drug effects; D000431:Ethanol; D006801:Humans; D031324:Juglans / Q000737:chemistry*; D015603:Keratinocytes / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009754:Nuts / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids; D014466:Ultraviolet Rays; D020122:tert-Butylhydroperoxide / Q000009:adverse effects
chemical_list: D010936:Plant Extracts; D000431:Ethanol; D020122:tert-Butylhydroperoxide; D006899:Mixed Function Oxygenases
keywords: DNA damage; Juglans regia L.; Keratinocytes; Oxidative stress