title: The TLR2 Binding Neisserial Porin PorB Enhances Antigen Presenting Cell Trafficking and Cross-presentation.
abstract: TOLL-like receptor (TLR) ligands activate both innate and adaptive immune cells, while modulating the cellular immune response. The outer membrane protein (OMP) from Neisseria meninigitidis, PorB, is a naturally occurring TLR2 ligand and functions as an adjuvant. Here, we demonstrate that PorB increases the level of OVA in the endo-/lysosomal cellular compartment of BMDCs, increases antigen presenting cell (APC) trafficking to draining lymph nodes, and enhances antigen cross-presentation. PorB is capable of mounting an antigen specific T cell response by efficiently stimulating antigen cross-presentation in vivo and in vitro assessed by BMDC OT-I cocultivation assays. The enhanced antigen cross-presentation and the increased APC recruitment to secondary lymphoid tissues expand the scope of known adjuvant effects of PorB on the immune system. Our findings lead to a better understanding of how TLR-ligand based adjuvants can alter and modulate immune responses.
authors.affiliation: Section of Infectious Diseases, Department of Medicine, Boston Medical Center, Boston, USA.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Reiser
authors.affiliation: Department of Microbiology, Boston University School of Medicine, Boston, USA.|Division of Immunology, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, USA.
authors.forename: Munir M
authors.initials: MM
authors.lastname: Mosaheb
authors.affiliation: Section of Infectious Diseases, Department of Medicine, Boston Medical Center, Boston, USA.
authors.forename: Christina
authors.identifier: http://orcid.org/0000-0001-6217-4063
authors.initials: C
authors.lastname: Lisk
authors.affiliation: Department of Microbiology, Boston University School of Medicine, Boston, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Platt
authors.affiliation: Department of Microbiology, Boston University School of Medicine, Boston, USA. lwetzler@bu.edu.|Section of Infectious Diseases, Department of Medicine, Boston Medical Center, Boston, USA. lwetzler@bu.edu.
authors.forename: Lee M
authors.initials: LM
authors.lastname: Wetzler
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology; D049109:Cell Proliferation; D045142:Cross-Priming / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005260:Female; D007114:Immunization; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008589:Meningococcal Infections / Q000276:immunology / Q000382:microbiology; D051379:Mice; D018345:Mice, Knockout; D009345:Neisseria meningitidis / Q000276:immunology; D018272:Porins / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D018272:Porins; D051195:Toll-Like Receptor 2; C057699:porin protein, Neisseria