title: Evaluation of calcium hydrogen carbonate mesoscopic crystals as a disinfectant for influenza A viruses.
abstract: In this study, the virucidal effect of a novel electrically charged disinfectant CAC-717 was investigated. CAC-717 is produced by applying an electric field to mineral water containing calcium hydrogen carbonate to generate mesoscopic crystals. Virus titration analysis showed a >3 log reduction of influenza A viruses after treatment with CAC-717 for 1 min in room temperature, while infectivity was undetectable after 15 min treatment. Adding bovine serum albumin to CAC-717 solution did not affect the disinfectant effect. Although CAC-717 is an alkaline solution (pH=12.39), upon contact with human tissue, its pH becomes almost physiological (pH 8.84) after accelerated electric discharge, which enables its use against influenza viruses. Therefore, CAC-717 may be used as a preventative measure against influenza A viruses and for biosecurity in the environment.
authors.affiliation: Research Institute for Animal Science in Biochemistry and Toxicology, Midori-ku, Sagamihara, Kanagawa 252-0132, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Nakashima
authors.affiliation: Research Institute for Animal Science in Biochemistry and Toxicology, Midori-ku, Sagamihara, Kanagawa 252-0132, Japan.
authors.forename: Masaomi
authors.initials: M
authors.lastname: Kawamoto
authors.affiliation: Research Institute for Animal Science in Biochemistry and Toxicology, Midori-ku, Sagamihara, Kanagawa 252-0132, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.affiliation: Mineral Activation Technical Research Center, Omuta, Fukuoka 836-0041, Japan.
authors.forename: Rumiko
authors.initials: R
authors.lastname: Onishi
authors.affiliation: Mineral Activation Technical Research Center, Omuta, Fukuoka 836-0041, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Furusaki
authors.affiliation: Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Osaki
authors.affiliation: Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Rikio
authors.initials: R
authors.lastname: Kirisawa
authors.affiliation: University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
authors.forename: Akikazu
authors.initials: A
authors.lastname: Sakudo
authors.affiliation: Research Center for Food Safety, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
mesh_terms: D002254:Carbonates / Q000494:pharmacology*; D003460:Crystallization; D004202:Disinfectants / Q000494:pharmacology*; D009980:Influenza A virus / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning
chemical_list: D002254:Carbonates; D004202:Disinfectants; C000624119:calcium hydrogen carbonate
keywords: antisepsis; biosecurity; calcium hydrogen carbonate; influenza virus; public health