title: Enhanced methane production by semi-continuous mesophilic co-digestion of potato waste and cabbage waste: Performance and microbial characteristics analysis.
abstract: Anaerobic granular sludge was used as an inoculum for co-digestion of potato waste (PW) and cabbage waste (CW) in batch and semi-continuous modes at 37±1°C for enhanced methane generation. Batch test results indicated that an equal proportion (1:1) by volatile solid was the optimal mixing ratio for co-digestion of PW and CW. Semi-continuous co-digestion process results showed that the stepwise increasing of the organic loading rates from 1.0 to 5.0kgVS/m3·d improved the methane yield from 224 to 360mL/g-VS. And the highest value was respectively 18.4% and 24.1% higher as compared to the mon-digestion of PW and CW. Further investigation with high-throughput sequencing analysis revealed that the enhanced methane generation was attributed to the partial shift from archaeal Methanosaeta to Methanosarcina and Methanobacterium, and from bacterial Firmicutes to Bacteroidetes and Proteobacteria. The volatile fatty acids concentration accounted for the modification of microbial communities.
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Mu
authors.affiliation: School of Resources & Environment, University of Jinan, Jinan 250022, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Hua
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China. Electronic address: zhangxd@sderi.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Energy Research Institute of Shandong Academy of Sciences, Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Xu
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D001937:Brassica; D008697:Methane / Q000096:biosynthesis; D012722:Sewage / Q000382:microbiology; D011198:Solanum tuberosum
chemical_list: D012722:Sewage; D008697:Methane
keywords: Anaerobic co-digestion; Batch and semi-continuous modes; Cabbage waste; Microbial community; Potato waste