title: Tuning Thermoresponsive Properties of Cationic Elastin-like Polypeptides by Varying Counterions and Side-Chains.
abstract: We report the synthesis of methionine-containing recombinant elastin-like polypeptides (ELPs) of different lengths that contain periodically spaced methionine residues. These ELPs were chemoselectively alkylated at all methionine residues to give polycationic derivatives. Some of these samples were found to possess solubility transitions in water, where the temperature of these transitions varied with ELP concentration, nature of the methionine alkylating group, and nature of the sulfonium counterions. These studies show that introduction and controlled spacing of methionine sulfonium residues into ELPs can be used as a means both to tune their solubility transition temperatures in water using a variety of different parameters and to introduce new side-chain functionality.
authors.affiliation: Université de Bordeaux/Bordeaux-INP, ENSCBP, CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629) , 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Rosine
authors.initials: R
authors.lastname: Petitdemange
authors.affiliation: Université de Bordeaux/Bordeaux-INP, ENSCBP, CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629) , 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Garanger
authors.affiliation: Université de Bordeaux/Bordeaux-INP, ENSCBP, CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629) , 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Laure
authors.identifier: 0000-0003-2351-8796
authors.initials: L
authors.lastname: Bataille
authors.affiliation: Université de Bordeaux/Bordeaux-INP, CNRS, Chimie et Biologie des Membranes et des Nano-objets (UMR5248) , Allée Geoffroy Saint Hilaire, Pessac 33600, France.
authors.forename: Katell
authors.initials: K
authors.lastname: Bathany
authors.affiliation: Université de Bordeaux/Bordeaux-INP, ENSCBP, CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629) , 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Garbay
authors.affiliation: Department of Chemistry and Biochemistry, and Department of Bioengineering, University of California , Los Angeles, California 90095, United States.
authors.forename: Timothy J
authors.identifier: 0000-0002-0594-5025
authors.initials: TJ
authors.lastname: Deming
authors.affiliation: Université de Bordeaux/Bordeaux-INP, ENSCBP, CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629) , 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Sébastien
authors.identifier: 0000-0003-0465-8603
authors.initials: S
authors.lastname: Lecommandoux
mesh_terms: D002412:Cations / Q000737:chemistry*; D004549:Elastin / Q000737:chemistry*; D008715:Methionine / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012995:Solubility; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D002412:Cations; D010455:Peptides; D014867:Water; D004549:Elastin; D008715:Methionine