title: Microbial Community Structure in a Serpentine-Hosted Abiotic Gas Seepage at the Chimaera Ophiolite, Turkey.
abstract: The surface waters at the ultramafic ophiolitic outcrop in Chimaera, Turkey, are characterized by high pH values and high metal levels due to the percolation of fluids through areas of active serpentinization. We describe the influence of the liquid chemistry, mineralogy, and H2and CH4levels on the bacterial community structure in a semidry, exposed, ultramafic environment. The bacterial and archaeal community structures were monitored using Illumina sequencing targeting the 16S rRNA gene. At all sampling points, four phyla,Proteobacteria,Actinobacteria,Chloroflexi, andAcidobacteria, accounted for the majority of taxa. Members of theChloroflexiphylum dominated low-diversity sites, whereasProteobacteriadominated high-diversity sites. Methane, nitrogen, iron, and hydrogen oxidizers were detected as well as archaea and metal-resistant bacteria.IMPORTANCEOur study is a comprehensive microbial investigation of the Chimaera ophiolite. DNA has been extracted from 16 sites in the area and has been studied from microbial and geochemical points of view. We describe a microbial community structure that is dependent on terrestrial, serpentinization-driven abiotic H2, which is poorly studied due to the rarity of these environments on Earth.
authors.affiliation: Department of Geological Sciences, Stockholm University, Stockholm, Sweden anna.neubeck@geo.su.se.
authors.forename: Anna
authors.identifier: http://orcid.org/0000-0002-1291-5529
authors.initials: A
authors.lastname: Neubeck
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Bettina
authors.initials: B
authors.lastname: Müller
authors.affiliation: Swedish Museum of Natural History, Department of Paleobiology and Nordic Center for Earth Evolution, Stockholm, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Ivarsson
authors.affiliation: Department of Geological Engineering, Istanbul University, Istanbul, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Hosgörmez
authors.affiliation: Department of Geological Engineering, Istanbul University, Istanbul, Turkey.
authors.forename: Dogacan
authors.initials: D
authors.lastname: Özcan
authors.affiliation: Department of Geological Sciences, Stockholm University, Stockholm, Sweden.
authors.forename: Curt
authors.initials: C
authors.lastname: Broman
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Schnürer
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D006859:Hydrogen / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D010802:Phylogeny; D014421:Turkey
chemical_list: D008670:Metals; D006859:Hydrogen; D008697:Methane
keywords: Archaea; bacteria; hydrogen; microbial community structure; ophiolite; serpentinization