title: Expression and localization of Yap and Taz during development of the mandibular first molar in rats.
abstract: Yes-associated protein (Yap) and transcriptional coactivator with PDZ-binding motif (Taz) are two downstream factors in the Hippo signaling pathway. Yap and Taz participate in regulating organ size, stem cell self-renewal, proliferation and differentiation. We investigated the spatial-temporal expression and relative expression levels of Yap and Taz using immunohistochemistry and real-time polymerase chain reaction. We found Yap and Taz in the oral epithelium and mesenchyme at embryonic (E) day 14.5 (E14.5) and E16.5. By E18.5, Yap and Taz were detected in the dental papilla and the entire enamel organ. At postnatal (P) day 0 (PN0), PN3 and PN7, Yap and Taz expression was localized in ameloblasts, odontoblasts and stratum intermedium. Yap and Taz were expressed in Hertwig's epithelial root sheath (HERS) at PN7. At PN3, PN7 and PN14, Yap was detected in the enamel matrix. From PN21 to PN28, Yap and Taz were absent from differentiated ameloblasts, but they were expressed in odontoblasts. From PN0 to PN10, the Yap and Taz mRNA expression increased, then decreased. We found that Yap and Taz may influence the differentiation of ameloblasts and odontoblasts; they also may contribute to enamel mineralization, crown morphogenesis and root formation.
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: B Y
authors.initials: BY
authors.lastname: Sun
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Ji
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: X X
authors.initials: XX
authors.lastname: Wang
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.affiliation: a School of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue, Jinan , Shandong , P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wen
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism*; D008334:Mandible / Q000196:embryology*; D008963:Molar / Q000196:embryology*; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins; D000091102:YAP-Signaling Proteins
chemical_list: D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins; C000612286:WWTR1 protein, rat; D000091102:YAP-Signaling Proteins; C509425:Yap1 protein, rat
keywords: Hippo pathway; PDZ-binding motif; Yes-associated protein; differentiation; immunohistochemistry; molar development; rat; real-time quantitative polymerase chain reaction; transcriptional co-activator