title: Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma-mass spectrometry.
abstract: The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin-1. The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2of 0.9999, the peak area repeatability was 1.9% (n=7), and the detection limit was 14ngmL-1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia.
authors.forename: David P
authors.initials: DP
authors.lastname: Bishop
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia.
authors.forename: Lucas
authors.initials: L
authors.lastname: Blanes
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia.
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Wilson
authors.affiliation: Agilent Technologies, Santa Clara, CA, USA.
authors.forename: Thor
authors.initials: T
authors.lastname: Wilbanks
authors.affiliation: Agilent Technologies, Santa Clara, CA, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Killeen
authors.affiliation: University of California Davis, Davis, CA, USA.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Grimm
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia; Pathology NSW, Royal North Shore Hospital, St Leonards, NSW, 2065, Australia.
authors.forename: Ross
authors.initials: R
authors.lastname: Wenzel
authors.affiliation: Agnes Banks Equine Clinic, Agnes Banks, NSW, 2753, Australia.
authors.forename: Derek
authors.initials: D
authors.lastname: Major
authors.affiliation: School of Physical Sciences and Australian Centre for Research on Separation Science at the University of Tasmania in Hobart, Australia.
authors.forename: Mirek
authors.initials: M
authors.lastname: Macka
authors.affiliation: Teledyne Technologies, Thousand Oaks, CA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Clarke
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, 48149 Münster, Germany.
authors.forename: Robin
authors.initials: R
authors.lastname: Schmid
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia; School of Optometry and Vision Science, University of NSW, Australia.
authors.forename: Nerida
authors.initials: N
authors.lastname: Cole
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia. Electronic address: philip.doble@uts.edu.au.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Doble
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006736:Horses / Q000097:blood; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D014805:Vitamin B 12 / Q000097:blood*
chemical_list: D014805:Vitamin B 12
keywords: Chip HPLC; Cobalamin; Microfluidics; NanoLC-ICP–MS