title: Stoichiometry and Turnover of the Stator and Rotor.
abstract: Fluorescence imaging techniques using green fluorescent protein (GFP) and related fluorescent proteins are utilized to monitor and analyze a wide range of biological processes in living cells. Stepwise photobleaching experiments can determine the stoichiometry of protein complexes. Fluorescence recovery after photobleaching (FRAP) experiments can reveal in vivo dynamics of biomolecules. In this chapter, we describe methods to detect the subcellular localization, stoichiometry, and turnovers of stator and rotor components of the Salmonella flagellar motor.
authors.affiliation: Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan. yvm010@riken.jp.
authors.forename: Yusuke V
authors.initials: YV
authors.lastname: Morimoto
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Minamino
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D005407:Flagella / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000378:metabolism*; D038761:Photobleaching; D012475:Salmonella / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020409:Molecular Motor Proteins; D049452:Green Fluorescent Proteins
keywords: FRAP; Flagellar motor; Fluorescence imaging; Fluorescent protein; Stoichiometry; Subcellular localization; Turnover