title: Early γ-irradiation and subsequent storage of red cells in SAG-M additive solution potentiate energy imbalance, microvesiculation and susceptibility to stress-induced apoptotic cell death.
abstract: γ-Irradiation of red blood cell (RBC) concentrates prevents transfusion-associated graft-versus-host disease but may diminish RBC quality. Herein, we show that early γ-irradiation (25 Gy) of RBC units and their subsequent storage in SAG-M additive solution altered membrane microvesiculation, supernatant haemoglobin and cytosolic ATP. γ-Irradiation did not influence phosphatidylserine externalization, a marker of erythrocyte apoptotic cell death (eryptosis), in RBC stored for 42 days. However, shorter periods (4-21 days) of storage accentuated eryptosis in γ-irradiated RBC versus untreated RBCs following energy depletion, suggesting that γ-irradiated RBC is primed for stress-induced eryptosis during storage.
authors.affiliation: Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada.|Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: S M
authors.initials: SM
authors.lastname: Qadri
authors.affiliation: Centre for Innovation, Canadian Blood Services, Vancouver, BC, Canada.|Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.affiliation: Centre for Innovation, Canadian Blood Services, Vancouver, BC, Canada.|Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Schubert
authors.affiliation: Centre for Innovation, Canadian Blood Services, Vancouver, BC, Canada.|Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
authors.forename: D V
authors.initials: DV
authors.lastname: Devine
authors.affiliation: Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada.|Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: W P
authors.identifier: http://orcid.org/0000-0002-5870-8189
authors.initials: WP
authors.lastname: Sheffield
mesh_terms: D017209:Apoptosis; D001793:Blood Preservation; D004912:Erythrocytes / Q000502:physiology* / Q000528:radiation effects; D000067128:Extracellular Vesicles / Q000378:metabolism; D005720:Gamma Rays; D006801:Humans; D012996:Solutions
chemical_list: D012996:Solutions
keywords: RBC storage lesion; eryptosis; γ-Irradiation