title: Tissue sulfomucin and sialomucin content in colon mucosa without intestinal transit subjected to intervention with Curcuma longa (curcumin).
abstract: To measure the tissue sulfomucin and sialomucin content of the colon mucosa without fecal flow, subjected to intervention with curcumin, and the influence of the concentration used and the intervention time.
  Thirty-six rats were subjected to proximal right colostomy and distal mucous fistula. They were divided into two groups according to whether sacrifice was performed two or four weeks after the intervention. Each group was divided into three subgroups according to the enema applied daily: saline alone; curcumin at 50 mg/kg/day or curcumin at 200 mg/kg/day. Acid mucins were diagnosed using the Alcian blue technique. The mucin content was quantified by means of computer-assisted image analysis. The significance level of 5% was used throughout (p < 0.05).
  There were dose-related increases in the quantities of sulfomucins in the animals subjected to interventions with curcumin, both after two weeks (p < 0.00001) and after four weeks (p < 0.00001). There were increases in sialomucin quantity that were concentration-related (p < 0.00001) and time-related (p < 0.00001).
  Curcumin enemas increase the quantity of acid mucins in the intestinal flow in the excluded colon, with dose and time dependency.
authors.affiliation: Fellow Master degree, Postgraduate Program in Surgical Sciences, Universidade Estadual de Campinas (UNICAMP), Brazil. Intellectual and scientific content of the study; acquisition, analysis and interpretation of data.
authors.forename: Antonio José Tiburcio
authors.initials: AJ
authors.lastname: Alves
authors.affiliation: PhD, Assistant Professor, Division of Pathology, Universidade São Francisco University (USF), Bragança Paulista-SP, Brazil. Acquisition, analysis and interpretation of data; histological examinations.
authors.forename: José Aires
authors.initials: JA
authors.lastname: Pereira
authors.affiliation: Undergraduate student, Medical School, USF, Bragança Paulista-SP, Brazil. PIBIQ/CNPq scholarship holder. Acquisition, analysis and interpretation of data.
authors.forename: Adrieli Heloísa Campardo
authors.initials: AH
authors.lastname: Pansani
authors.affiliation: PhD, Assistant Professor, Division of Nutrition, UNICAMP, Campinas-SP, Brazil. Acquisition and interpretation of data, statistical analysis.
authors.forename: Daniela Oliveira
authors.initials: DO
authors.lastname: Magro
authors.affiliation: PhD, Associate Professor, Head, Department of Surgery, UNICAMP, Campinas-SP, Brazil. Acquisition, analysis and interpretation of data; manuscript preparation.
authors.forename: Cláudio Saddy Rodrigues
authors.initials: CS
authors.lastname: Coy
authors.affiliation: PhD, Associate Professor, USF and UNICAMP, Brazil. Grant from National Council for Scientific and Technological Development (CNPq), Ministry of Science, Scientific Productivity, Level 2. Intellectual and scientific content of the study, conception and design of the study, manuscript writing.
authors.forename: Carlos Augusto Real
authors.initials: CA
authors.lastname: Martinez
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy / Q000473:pathology; D003106:Colon / Q000737:chemistry* / Q000187:drug effects* / Q000473:pathology; D003125:Colostomy; D030024:Curcuma; D004733:Enema / Q000379:methods; D005243:Feces; D005772:Gastrointestinal Transit / Q000187:drug effects; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009077:Mucins / Q000032:analysis* / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D010938:Plant Oils / Q000008:administration & dosage; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D052116:Sialomucins / Q000032:analysis* / Q000187:drug effects; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D009077:Mucins; D010936:Plant Extracts; D010938:Plant Oils; D052116:Sialomucins; C012564:sulfomucin; C012596:turmeric extract