title: Automated glycan assembly using the Glyconeer 2.1 synthesizer.
abstract: Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.|Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany.
authors.forename: Heung Sik
authors.identifier: 0000-0003-2326-7923
authors.initials: HS
authors.lastname: Hahm
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.|GlycoUniverse GmbH & Co. KGaA, 14195 Berlin, Germany.
authors.forename: Mark K
authors.initials: MK
authors.lastname: Schlegel
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
authors.forename: Mattan
authors.identifier: 0000-0002-1038-8104
authors.initials: M
authors.lastname: Hurevich
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Eller
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.|Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schuhmacher
authors.affiliation: Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany.|Fritz Haber Institute of the Max Planck Society, 14195 Berlin, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Hofmann
authors.affiliation: Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany.|Fritz Haber Institute of the Max Planck Society, 14195 Berlin, Germany.
authors.forename: Kevin
authors.initials: K
authors.lastname: Pagel
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany; peter.seeberger@mpikg.mpg.de.|Institute of Chemistry and Biochemistry, Free University Berlin, 14195 Berlin, Germany.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
keywords: Glyconeer; automated synthesis; glycobiology; glycochemistry; oligosaccharide synthesis