title: Generation and characterization of human iPSC line from CD34+cells isolated from umbilical cord blood belonging to Indian origin.
abstract: We describe here the reprogramming of CD34+cells isolated from umbilical cord blood obtained after full term delivery of a healthy female child of Indian origin. The cells were nucleofected by episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD (negative mutation in p53). Colonies were identified by alkaline phosphatase staining and characterized for expression of pluripotency markers at protein level by immunofluorescence, flow cytometry and at transcript level by PCR. Genomic stability of the cell line was checked by G-banded karyotype. The ability to differentiate to endoderm, mesoderm and ectoderm in vitro was confirmed by immunofluorescence staining.
authors.affiliation: Stem Cell Laboratory, National Centre for Cell Science, NCCS Complex, University of Pune Campus, Ganeshkhind, Pune 411007, India.
authors.forename: Sophia
authors.initials: S
authors.lastname: Fernandes
authors.affiliation: Stem Cell Laboratory, National Centre for Cell Science, NCCS Complex, University of Pune Campus, Ganeshkhind, Pune 411007, India.
authors.forename: Manasi
authors.initials: M
authors.lastname: Talwadekar
authors.affiliation: Obstetrics and Gynecology at Obstetrics and Gynecology Department, AFMC, Pune 411040, India.
authors.forename: Raju
authors.initials: R
authors.lastname: Agarwal
authors.affiliation: Medicine & Haematology, Armed Forces Medical College (AFMC), Pune 411040, India.
authors.forename: Velu
authors.initials: V
authors.lastname: Nair
authors.affiliation: Stem Cell Laboratory, National Centre for Cell Science, NCCS Complex, University of Pune Campus, Ganeshkhind, Pune 411007, India.
authors.forename: Vaijayanti
authors.initials: V
authors.lastname: Kale
authors.affiliation: Stem Cell Laboratory, National Centre for Cell Science, NCCS Complex, University of Pune Campus, Ganeshkhind, Pune 411007, India. Electronic address: lslimaye@nccs.res.in.
authors.forename: Lalita
authors.initials: L
authors.lastname: Limaye
mesh_terms: D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D065150:Cellular Reprogramming; D004475:Ectoderm / Q000378:metabolism / Q000473:pathology; D004707:Endoderm / Q000378:metabolism / Q000473:pathology; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007194:India; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008648:Mesoderm / Q000378:metabolism / Q000473:pathology; D008856:Microscopy, Fluorescence
chemical_list: D018952:Antigens, CD34; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4