title: Optimization of Glycyrrhiza polysaccharide liposome by response surface methodology and its immune activities.
abstract: Glycyrrhiza polysaccharide liposome (GPSL) was prepared by reverse-phase evaporation method and optimized with response surface methodology. As a result, the optimum preparation conditions were as follows: the ratio of soybean phosphatide to Glycyrrhiza polysaccharide (GPS) of 24:1, temperature of drug incubation of 46°C, the ultrasound time of 16min. Confirmation experiments and transmission electron micrograph (SEM) exhibited the entrapment efficiency of liposome as 78.33±0.25%, with spherical shape and uniform sizes. Furthermore, proliferation assay, allogeneic mixed lymphocyte reaction and supernatant cytokines of chBM-DCs were performed by MTT and ELISA methods to investigate the immune-modulating effects of GPSL and GPS. The results showed that GPSL could significantly promote the proliferation of immature chBM-DCs, enhance the ability of mature chBM-DCs to induce T-cell proliferation and modulate the mature chBM-DCs to secret cytokines such as IL-2, IFN-γ and IL-10. These evidences indicated that the immune-modulating activity of GPS was significantly improved after encapsulated with liposome.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China. Electronic address: wuyi2001cn@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yi
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China; College of Veterinary Medicine, South China Agricultural University,483 Wushan Road, Guangzhou 510642, PR China; Institute of Military Veterinary Medicine, Academy of Military Medical Science,666 Liuying West Road, Changchun 130122, PR China.
authors.forename: Entao
authors.initials: E
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Youying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
authors.forename: Peijuan
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Shandong University of Traditional Chinese Medicine, #4655 Daxue Road, Jinan 250355, Shandong Province, PR China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, #1 Weigang, Nanjing 210095, Jiangsu Province, PR China. Electronic address: dywang@njau.edu.cn.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006035:Glycyrrhiza / Q000737:chemistry*; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000494:pharmacology*; D008081:Liposomes; D008297:Male; D008962:Models, Theoretical; D011134:Polysaccharides / Q000008:administration & dosage* / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013696:Temperature; D000069453:Ultrasonic Waves
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D008081:Liposomes; D011134:Polysaccharides
keywords: Chloroform (PubChem CID: 6212); Cholesterol (PubChem CID: 5997); Dimethyl sulfoxide (PubChem CID: 679); Glycyrrhiza polysaccharide liposome; Immune activities; Lipopolysaccharide (PubChem CID: 11970143); MTT (PubChem CID: 64965); Phenol (PubChem CID: 996); Preparation and optimization; Tween-80 (PubChem CID: 5281955); α-d-Galactose (PubChem CID: 439357); α-d-Glucose (PubChem CID: 79025); α-l-Arabinose (PubChem CID: 439731)