title: Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission.
abstract: An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea. In this study, we sequenced and characterized the complete genome of strain KKL-117. On full-genomic analysis, strain KKL-117 was found to have the following genotype constellation: G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The non-G/P genotype constellation of this strain (I5-R1-C1-M1-A8-N1-T1-E1-H1) is commonly shared with rotavirus strains from pigs. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin. Our observations provide important insights into the dynamic interactions between human and porcine rotavirus strains.
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komoto
authors.affiliation: Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Ratana
authors.initials: R
authors.lastname: Tacharoenmuang
authors.affiliation: Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Ratigorn
authors.initials: R
authors.lastname: Guntapong
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Ide
authors.affiliation: Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Phakapun
authors.initials: P
authors.lastname: Sinchai
authors.affiliation: Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Sompong
authors.initials: S
authors.lastname: Upachai
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Piyanit
authors.initials: P
authors.lastname: Tharmaphornpilas
authors.affiliation: Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Sangkitporn
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000635:transmission / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013785:Thailand; D015047:Zoonoses / Q000635:transmission / Q000821:virology