title: 25-Hydroxyvitamin D and Peripheral Immune Mediators: Results from Two Nationwide Danish Pediatric Cohorts.
abstract: (1) Background: We aimed to examine if 25-hydroxyvitamin D (25(OH)D) was related to the peripheral immunological and inflammatory signature both at birth, and in newly diagnosed patients with childhood type 1 diabetes (T1D) and their healthy controls; (2) Methods: The birth cohort consisted of 470 patients and 500 healthy controls. Dried blood samples were collected from the neonates in the period 1981-1999. The newly diagnosed cohort consisted of 460 patients and 453 siblings. Serum samples were collected in the period 1997-2005. A variety of peripheral immune mediators were measured and compared to total 25(OH)D levels (25(OH)D₂ + 25(OH)D₃). For each immune mediator, the relative change (RC) in the mean level was modeled by robust log-normal regression and correction for multiple testing was performed; (3) Results: Two associations were identified; there was a negative association between 25(OH)D (10 nmol/L increase) and leptin (RC (95% confidence interval (CI)), 0.98 (0.96; 1.00)), and a positive association between 25(OH)D (10 nmol/L increase) and the chemokine, chemokine (c-x-c motif) ligand (CXCL) 8 (RC (95% CI), 1.07 (1.01; 1.13)); (4) Conclusion: CXCL8 and leptin have significant associations with levels of 25(OH)D in the newly diagnosed cohort. These results do not indicate a strong influence of 25(OH)D on the peripheral immunological or inflammatory signature.
authors.affiliation: Copenhagen Diabetes Research Center (CPH-DIRECT), Department of Pediatrics, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, 2730 Herlev, Denmark. s.u.thorsen@gmail.com.
authors.forename: Steffen U
authors.initials: SU
authors.lastname: Thorsen
authors.affiliation: Department of Public Health, Section of Biostatistics, University of Copenhagen, Copenhagen, Oester Farimagsgade 5, 1710 Copenhagen K, Denmark. pipper@sund.ku.dk.
authors.forename: Christian B
authors.initials: CB
authors.lastname: Pipper
authors.affiliation: Department of Congenital Disorders, Center for Neonatal Screening, Statens Serum Institute, Artillerivej 5, 2300 Copenhagen S, Denmark. ksk@ssi.dk.
authors.forename: Kristin
authors.initials: K
authors.lastname: Skogstrand
authors.affiliation: Copenhagen Diabetes Research Center (CPH-DIRECT), Department of Pediatrics, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, 2730 Herlev, Denmark. flemming.pociot.01@regionh.dk.|Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark. flemming.pociot.01@regionh.dk.
authors.forename: Flemming
authors.initials: F
authors.lastname: Pociot
authors.affiliation: Copenhagen Diabetes Research Center (CPH-DIRECT), Department of Pediatrics, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, 2730 Herlev, Denmark. Jannet.Svensson@regionh.dk.|Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark. Jannet.Svensson@regionh.dk.
authors.forename: Jannet
authors.initials: J
authors.lastname: Svensson
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D003718:Denmark; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000175:diagnosis; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000097:blood*; D007231:Infant, Newborn; D016209:Interleukin-8 / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D044465:White People
chemical_list: C508606:CXCL8 protein, human; D007155:Immunologic Factors; D016209:Interleukin-8; D020738:Leptin; D014807:Vitamin D; C104450:25-hydroxyvitamin D
keywords: Adolescent; C-reactive protein; Child; Cytokines/immunology; Diabetes Mellitus, Type 1; Infant, Newborn; TREM1, human; mannose-binding lectin; vitamin D