title: Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy.
abstract: The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the two dimensional second harmonic (SH) Stokes images of starch granules. The SH signal from hydrated and dry starch on heating differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan. nirmaluva@gmail.com.|Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India. nirmaluva@gmail.com.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Mazumder
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan.
authors.forename: Lu Yun
authors.initials: LY
authors.lastname: Xiang
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan.|Key Laboratory of Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan. fjkao@ym.edu.tw.
authors.forename: Fu-Jen
authors.initials: FJ
authors.lastname: Kao