title: Therapeutic effects of human amnion-derived mesenchymal stem cell transplantation and conditioned medium enema in rats with trinitrobenzene sulfonic acid-induced colitis.
abstract: Cell therapy with mesenchymal stem cells (MSCs) is expected to provide a new strategy for the treatment of inflammatory bowel disease (IBD). Large amounts of MSCs can be obtained from human amnion. Therefore, we investigated the effect of transplantation of human amnion-derived MSCs (hAMSCs) or enema of conditioned medium (CM) from hAMSCs into rats with 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. In the first experiment, 10-week-old male Sprague-Dawley rats were intravenously injected with hAMSCs (1 × 106cells) 3 h after rectal administration of TNBS (45 mg/kg). In the second experiment, rats with TNBS-induced colitis received CM by enema into the colon for 3 days. Colitis was investigated by endoscopy, histology, immunohistochemistry, and by measuring mRNA expression of inflammatory mediators. Administration of hAMSCs or CM enema significantly improved the endoscopic score. In addition, these two interventions resulted in significantly decreased infiltration of neutrophils and monocytes/macrophages and decreased expression levels of TNF-α, CXCL1, and CCL2. In conclusion, transplantation of hAMSCs and CM enema provided significant improvement in rats with TNBS-induced colitis. CM from hAMSCs and hAMSCs may be new strategies for the treatment of IBD.
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University Sapporo, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Miyamoto
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University Sapporo, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Ohnishi
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University Sapporo, Japan.
authors.forename: Reizo
authors.initials: R
authors.lastname: Onishi
authors.affiliation: Laboratory of Pathophysiology and Therapeutics, Faculty of Pharmaceutical Sciences, Hokkaido University Sapporo, Japan.
authors.forename: Ikuki
authors.initials: I
authors.lastname: Tsuchiya
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University Sapporo, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hosono
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University Sapporo, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Katsurada
authors.affiliation: Department of Transfusion Medicine and Cell Therapy, Hyogo College of Medicine Nishinomiya, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamahara
authors.affiliation: Laboratory of Pathophysiology and Therapeutics, Faculty of Pharmaceutical Sciences, Hokkaido University Sapporo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeda
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University Sapporo, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
keywords: Mesenchymal stem cells; amnion; colitis; conditioned medium; trinitrobenzene sulfonic acid