title: Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare.
abstract: Foeniculum vulgareMill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results ofFoeniculum vulgareelutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity onPseudomonas aeruginosawith a diameter of a zone of inhibition of 16 mm and IC50value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life,Foeniculum vulgareseeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia. raalakeel@ksu.edu.sa.
authors.forename: Raid
authors.initials: R
authors.lastname: Al Akeel
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia. ayeshamateen@gmail.com.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Mateen
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia. rabbanisyd@gmail.com.
authors.forename: Rabbani
authors.initials: R
authors.lastname: Syed
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia. abalyousef@ksu.edu.sa.
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Alyousef
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. rafiskm@gmail.com.
authors.forename: Mohammed Rafi
authors.initials: MR
authors.lastname: Shaik
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D030001:Foeniculum / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D010940:Plant Proteins
keywords: Foeniculum vulgare; antimicrobial proteins; ion exchange chromatography