title: Study on the apoptosis mediated by cytochrome c and factors that affect the activation of bovine longissimus muscle during postmortem aging.
abstract: This study investigates whether bovine longissimus muscle cell apoptosis occurs during postmortem aging and whether apoptosis is dependent on the mitochondria pathway. This study also determines the apoptosis process mediated by cytochrome c after its release from mitochondria and the factors that affect the activation processes. Results indicate that apoptotic nuclei were detected at 12 h postmortem. Cytochrome c release from the mitochondria to the cytoplasm activated the caspase-9 and caspase-3 at early postmortem aging and the activation of caspase-9 occurs before the activation of caspase-3. The pH level decreased during the first 48 h postmortem, whereas the mitochondria membrane permeability increased from 6 to 12 h. Results demonstrate that an apoptosis process of bovine muscle occurred during postmortem aging. Apoptosis was dependent on the mitochondria pathway and occurred at early postmortem aging. Increased mitochondria membrane permeability and low pH are necessary conditions for the release of cytochrome c during postmortem aging.
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China. yuqunlihh@163.com.
authors.forename: Qunli
authors.initials: Q
authors.lastname: Yu
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Chongqing Heng Du Agricultural Development Co., Ltd., Fengdu, 408200, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.affiliation: Shandong Lorain Corporation Co., Ltd., Linyi, 276600, China.
authors.forename: Guangxing
authors.initials: G
authors.lastname: Han
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D009132:Muscles / Q000378:metabolism* / Q000473:pathology*; D010539:Permeability; D011180:Postmortem Changes; D000069466:Red Meat
chemical_list: D045304:Cytochromes c; D020169:Caspases
keywords: Apoptosis; Bovine longissimus muscle; Cytochrome c-mediated; Mitochondria; Postmortem aging