title: Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic β-glucosidases with industrial potential.
abstract: The β-glucosidases are important enzymes employed in a large number of processes and industrial applications, including biofuel production from biomass. Therefore, in this study, we reported for the first time the photobiosynthesis of stable and functional silver/silver chloride nanoparticles (Ag/AgCl-NPs) using two hyperthermostable bacterial β-glucosidases with industrial potential. The syntheses were straightforward and rapid processes carried out by mixing β-glucosidase and silver nitrate (in buffer 10mM Tris-HCl, pH 8) under irradiation with light (over a wavelength range of 450-600nm), therefore, compatible with the green chemistry procedure. Synthesized Ag/AgCl-NPs were characterized using a series of physical techniques. Absorption spectroscopy showed a strong absorption band centered at 460nm due to surface plasmon resonance of the Ag-NPs. X-ray diffraction analysis revealed that the Ag/AgCl-NPs were purely crystalline in nature. Under electron microscopy, Ag/AgCl-NPs of variable diameter ranging from 10 to 100nm can be visualized. Furthermore, electron microscopy, zeta potential and Fourier transform infrared spectroscopy results confirmed the presence of β-glucosidases coating and stabilizing the Ag/AgCl-NPs. Finally, the results showed that the enzymatic activities were maintained in the β-glucosidases assisted Ag/AgCl-NPs. The information described here should provide a useful basis for future studies of β-glucosidases assisted Ag/AgCl-NPs, including biotechnological applications.
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
authors.forename: Juscemácia N
authors.initials: JN
authors.lastname: Araújo
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
authors.forename: Aryane
authors.initials: A
authors.lastname: Tofanello
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
authors.forename: Viviam M
authors.initials: VM
authors.lastname: da Silva
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
authors.forename: Juliana A P
authors.initials: JAP
authors.lastname: Sato
authors.affiliation: Programa de Processos Tecnológicos e Ambientais, Universidade de Sorocaba (UNISO), Sorocaba, SP, Brazil.
authors.forename: Fabio M
authors.initials: FM
authors.lastname: Squina
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
authors.forename: Iseli L
authors.initials: IL
authors.lastname: Nantes
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), Santo André, SP, Brazil. Electronic address: wanius.garcia@ufabc.edu.br.
authors.forename: Wanius
authors.initials: W
authors.lastname: Garcia
mesh_terms: D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000378:metabolism / Q000528:radiation effects; D007221:Industry; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D012834:Silver / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018030:Silver Compounds; D012834:Silver; D001617:beta-Glucosidase; C037548:silver chloride
keywords: Green synthesis; Hyperthermostable β-glucosidase; Nanoparticles