title: In vitro larvicidal effect of a hydroalcoholic extract from Acacia cochliacantha leaf against ruminant parasitic nematodes.
abstract: The aim of this study was to evaluate the in vitro lethal effect of a hydroalcoholic extract (HAE) from Acacia cochliacantha leaf against three gastrointestinal nematodes species (Haemonchus contortus, H. placei and Cooperia punctata) of domestic ruminants. The HAE was assessed using five concentrations: 100, 125, 175, 150 and 200 mg/ml; 0.5% Ivermectin was used as a positive control and distilled water, as negative control. The data were normalized using the square root and analysed with a completely randomized design through ANOVA analysis using the general lineal model (GLM) of the SAS program. The HAE tannin content was determined through spectrophotometry (UV-visible) and the other major phenols, were identified by chromatographic processes. The results showed an in vitro larvicidal activity of the HAE against the three assessed nematode species with all assessed concentrations. A clear HAE increased concentration dependence effect was observed. The highest activity of the HAE was obtained at the highest concentration (close to 100%, P < 0.05). This result was similar to the one obtained with Ivermectin. On the other hand, the chemical analysis of HAE showed the presence of tannins, caffeoyls and coumaroyl derivates and quercetin as the main compounds. The results suggest that the HAE from this plant species possess in vitro anthelmintic properties. The identified compounds in this study would good candidates for further in vivo researches.
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET). Carretera Federal Cuernavaca-Cuautla, No. 8534 / Col. Progreso/ A.P. 206-CIVAC, C.P. 62550, Jiutepec, Morelos, Mexico. olmedo.agustin@inifap.gob.mx.
authors.forename: Agustín
authors.initials: A
authors.lastname: Olmedo-Juárez
authors.affiliation: Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carr. Fed. Toluca-Tejupilco, CP 51300, Temascaltepec, Estado de México, Mexico.
authors.forename: Rolando
authors.initials: R
authors.lastname: Rojo-Rubio
authors.affiliation: Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, CP 62790, Xochitepec, Morelos, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zamilpa
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET). Carretera Federal Cuernavaca-Cuautla, No. 8534 / Col. Progreso/ A.P. 206-CIVAC, C.P. 62550, Jiutepec, Morelos, Mexico.
authors.forename: Pedro
authors.initials: P
authors.lastname: Mendoza de Gives
authors.affiliation: Estación Experimental de Pastos y Forrajes Indio Hatuey, Universidad de Matanzas, Central España Republicana, CP 44280, Matanzas, Cuba.
authors.forename: Javier
authors.initials: J
authors.lastname: Arece-García
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID PAVET). Carretera Federal Cuernavaca-Cuautla, No. 8534 / Col. Progreso/ A.P. 206-CIVAC, C.P. 62550, Jiutepec, Morelos, Mexico.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: López-Arellano
authors.affiliation: Centro de Enseñanza Investigación y Extensión en Ganadería Tropical, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Km 5.5 Carretera Federal Tlapacoyan-Martínez de la Torre, C.P. 93600, Veracruz, Mexico.
authors.forename: Elke
authors.initials: E
authors.lastname: von Son-de Fernex
mesh_terms: D000045:Acacia / Q000737:chemistry; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006189:Haemonchus / Q000187:drug effects*; D066298:In Vitro Techniques; D007814:Larva / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017168:Rhabditida / Q000187:drug effects*; D012418:Ruminants / Q000469:parasitology
chemical_list: D000969:Antinematodal Agents; D010936:Plant Extracts
keywords: Acacia cochliacantha; Cooperia; Flavonoids; Haemonchus; Nematodes; Tannins