title: Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal.
abstract: We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington, United StatesbTianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Li
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington, United StatesbTianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin, China.
authors.forename: Ruikang
authors.initials: R
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D001831:Body Temperature; D002196:Capillaries / Q000000981:diagnostic imaging* / Q000502:physiology; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology*; D006439:Hemodynamics; D007834:Lasers; D051379:Mice; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D010477:Perfusion; D012542:Scattering, Radiation