title: Rce1, a novel transcriptional repressor, regulates cellulase gene expression by antagonizing the transactivator Xyr1 in Trichoderma reesei.
abstract: Cellulase gene expression in the model cellulolytic fungus Trichoderma reesei is supposed to be controlled by an intricate regulatory network involving multiple transcription factors. Here, we identified a novel transcriptional repressor of cellulase gene expression, Rce1. Disruption of the rce1 gene not only facilitated the induced expression of cellulase genes but also led to a significant delay in terminating the induction process. However, Rce1 did not participate in Cre1-mediated catabolite repression. Electrophoretic mobility shift (EMSA) and DNase I footprinting assays in combination with chromatin immunoprecipitation (ChIP) demonstrated that Rce1 could bind directly to a cbh1 (cellobiohydrolase 1-encoding) gene promoter region containing a cluster of Xyr1 binding sites. Furthermore, competitive binding assays revealed that Rce1 antagonized Xyr1 from binding to the cbh1 promoter. These results indicate that intricate interactions exist between a variety of transcription factors to ensure tight and energy-efficient regulation of cellulase gene expression in T. reesei. This study also provides important clues regarding increased cellulase production in T. reesei.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Fanglin
authors.initials: F
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Guolei
authors.initials: G
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan, Shandong, 250100, P. R. China.
authors.forename: Weifeng
authors.identifier: 0000-0003-2939-1212
authors.initials: W
authors.lastname: Liu
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D050436:Regulatory Elements, Transcriptional / Q000235:genetics; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase