title: Dynamic changes of paraoxonase 1 activity towards paroxon and phenyl acetate during coronary artery surgery.
abstract: Serum paraoxonase 1 (PON1), an enzyme associated with high - density lipoproteins (HDL) particles, inhibits the oxidation of serum lipoproteins and cell membranes. PON1 activity is lower in patients with atherosclerosis and in inflammatory diseases. The systemic inflammatory response provoked during cardiopulmonary bypass grafting may contribute to the development of postoperative complications. The aim of the present study was to estimate the dynamic changes in paraoxonase 1 (PON1) activity towards paraoxon and phenyl acetate during and after coronary artery surgery.
  
  
  
  Twenty six patients with coronary heart disease undergoing coronary artery bypass grafting (CABG) were enrolled into the study. Venous blood samples were obtained preoperatively, after aortic clumping, after the end of operation, at 6, 18, 30 and 48 h after operation. Paraoxonase activity was measured spectrophotometrically in 50 mM glycine/NaOH buffer (pH 10.5) containing 1.0 mM paraoxon, and 1.0 mM CaCl2. Arylesterase activity was measured in 20 mM TrisCl buffer (pH 8.0) containing 1 mM phenyl acetate and 1 mM CaCl2.
  
  
  
  PON1 activity toward paraoxon and phenyl acetate significantly decreased after aorta cross clumping and increased directly after operation. PON1 activity towards paraoxon in preoperative period and PON1 activity towards phenyl acetate in seventh stage of experiment tended to inversely correlate with the occurrence of postoperative complications.
  
  
  
  The paraoxonase 1 plasma activity is markedly reduced during CABG surgery.
authors.affiliation: Cardiology Department, Medical University of Lublin, ul. Jaczewskiego 8, 20-954, Lublin, Poland.|Internal Medicine in Nursing Department, Medical University of Lublin, ul. Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wysocka
authors.affiliation: Biochemistry and Molecular Biology Department, Medical University of Lublin, ul. Chodźki 1, 20-093, Lublin, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Cybulski
authors.affiliation: Biochemistry and Molecular Biology Department, Medical University of Lublin, ul. Chodźki 1, 20-093, Lublin, Poland.
authors.forename: Henryk
authors.initials: H
authors.lastname: Berbeć
authors.affiliation: Cardiology Department, Medical University of Lublin, ul. Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wysokiński
authors.affiliation: Cardiosurgery Department, Medical University of Lublin, ul. Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Stążka
authors.affiliation: Internal Medicine in Nursing Department, Medical University of Lublin, ul. Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Daniluk
authors.affiliation: Cardiology Department, Medical University of Lublin, ul. Jaczewskiego 8, 20-954, Lublin, Poland. zapolia@wp.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zapolski
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001026:Coronary Artery Bypass / Q000009:adverse effects; D003324:Coronary Artery Disease / Q000097:blood / Q000201:enzymology / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010261:Paraoxon / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011183:Postoperative Complications / Q000097:blood / Q000201:enzymology / Q000209:etiology; D012307:Risk Factors; D013379:Substrate Specificity; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000085:Acetates; D015415:Biomarkers; D010636:Phenols; C570634:phenyl acetate; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D010261:Paraoxon
keywords: Coronary artery bypass; Coronary heart disease; Paraoxonase 1