title: Monitoring yeast mitochondria with peroxiredoxin-based redox probes: the influence of oxygen and glucose availability.
abstract: Mitochondrially generated oxidants are believed to play important roles in both physiology and pathophysiology. Therefore, it is of significant interest to better understand the metabolic conditions leading to enhanced mitochondrial oxidant generation. Here, we investigate the influence of oxygen and glucose availability on the redox state of peroxiredoxin-based redox probes, expressed in the cytosol and mitochondrial matrix of yeast cells. We observe that the redox state of peroxiredoxin probes reflects the balance between dioxygen-dependent peroxide generation and glucose-dependent generation of reducing equivalents. The oxidative pentose phosphate pathway appears to be the dominant source of NADPH in the system under study.
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH Alliance , German Cancer Research Center (DKFZ) , Im Neuenheimer Feld 280, 69120 Heidelberg , Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pastor-Flores
authors.affiliation: Biochemistry and Molecular Biology , Heinrich-Buff-Ring 26-32, Justus Liebig University , 35392 Giessen , Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Becker
authors.affiliation: Division of Redox Regulation, DKFZ-ZMBH Alliance , German Cancer Research Center (DKFZ) , Im Neuenheimer Feld 280, 69120 Heidelberg , Germany.
authors.forename: Tobias P
authors.identifier: 0000-0003-1367-973X
authors.initials: TP
authors.lastname: Dick
keywords: genetically encoded redox probes; hydrogen peroxide; mitochondria; pentose phosphate pathway