title: The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis.
abstract: PurposeTo evaluate the effect of mesenchymal stem cells (MSCs) on fertility in experimental retrocervical endometriosis.MethodsA total of 27 New Zealand rabbits were divided into three groups: endometriosis, in which endometrial implants were created; mesenchymal, in which MSCs were applied in addition to the creation of endometrial implants; and control, the group without endometriosis. Fisher's exact test was performed to compare the dichotomous qualitative variables among the groups. The quantitative variables were compared by the nonparametric Mann-Whitney and Kruskal-Wallis tests. The Mann-Whitney test was used for post-hoc multiple comparison with Boniferroni correction.ResultsRegarding the beginning of the fertile period, the three groups had medians of 14 ± 12.7, 40 ± 5, and 33 ± 8.9 days respectively (p= 0.005). With regard to fertility (number of pregnancies), the endometriosis and control groups showed a rate of 77.78%, whereas the mesenchymal group showed a rate of 11.20% (p= 0.015). No differences in Keenan's histological classification were observed among the groups (p= 0.730). With regard to the macroscopic appearance of the lesions, the mesenchymal group showed the most pelvic adhesions.ConclusionThe use of MSCs in endometriosis negatively contributed to fertility, suggesting the role of these cells in the development of this disease.
authors.affiliation: Center for Health and Biological Sciences, Pontifícia Universidade Católica do Paraná (PUC-PR), Escola de Medicina, Curitiba, Paraná, Brazil.
authors.forename: Jaqueline Pedroso de
authors.initials: JP
authors.lastname: Abreu
authors.affiliation: Experimental Laboratory of Cell Culture, PUC-PR, Curitiba, Paraná, Brazil.
authors.forename: Carmen Lúcia Kuniyoshi
authors.initials: CLK
authors.lastname: Rebelatto
authors.affiliation: Center for Health and Biological Sciences, Pontifícia Universidade Católica do Paraná (PUC-PR), Escola de Medicina, Curitiba, Paraná, Brazil.
authors.forename: Carolina Antunes
authors.initials: CA
authors.lastname: Savari
authors.affiliation: Center for Health and Biological Sciences, Escola de Ciências Agrárias e Medicina Veterinária, PUC-PR, Curitiba, Paraná, Brazil.
authors.forename: Luiz Guilherme Achcar
authors.initials: LGA
authors.lastname: Capriglione
authors.affiliation: Center for Health and Biological Sciences, Escola de Ciências Agrárias e Medicina Veterinária, PUC-PR, Curitiba, Paraná, Brazil.
authors.forename: Lye
authors.initials: L
authors.lastname: Miyague
authors.affiliation: Center for Health and Biological Sciences, Pontifícia Universidade Católica do Paraná (PUC-PR), Escola de Medicina, Curitiba, Paraná, Brazil.
authors.forename: Lucia de
authors.initials: L
authors.lastname: Noronha
authors.affiliation: Department of Obstetrics and Gynecology, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brazil.
authors.forename: Vivian Ferreira do
authors.initials: VFD
authors.lastname: Amaral
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004715:Endometriosis / Q000209:etiology* / Q000473:pathology; D005260:Female; D007247:Infertility, Female / Q000209:etiology* / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000502:physiology; D011817:Rabbits; D002577:Uterine Cervical Diseases / Q000209:etiology* / Q000473:pathology