title: USP19 suppresses cellular type I interferon signaling by targeting TRAF3 for deubiquitination.
abstract: To investigate host factors that mediate the immune escape of enterovirus 71 (EV71) in the context of deubiquitinating enzymes.
  
  
  
  Utilize PCR array to screen candidate genes that may be involved in EV71-induced cellular antiviral immune responses, and utilize protein mass spectrometry analysis to identify the functional targets of the candidate regulator.
  
  
  
  EV71 infection induces the upregulation of ubiquitin-specific protease 19 (USP19) gene expression, which negatively regulates cellular antiviral type I interferon signaling. Additionally, we identify that USP19 suppresses cellular type I interferon signaling by targeting tumor necrosis factor receptor-associated factor 3 (TRAF3) molecule and decreasing TRAF3 ubiquitination of K63-linkage.
  
  
  
  This work suggests that USP19 is a previously unrecognized regulator employed by EV71 to evade host antiviral defenses.
authors.affiliation: Department of Laboratory Medicine, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, PR China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Laboratory Medicine, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Laboratory Medicine, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, PR China.|Department of Laboratory Medicine, Suzhou Municipal Hospital, Suzhou, Jiangsu 215002, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Cyrus Tang Hematology Center & Collaborative Innovation Center of Hematology, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
mesh_terms: D000072017:Deubiquitinating Enzymes / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D029821:Enterovirus A, Human / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D057131:Immune Evasion; D007111:Immunity, Cellular; D007113:Immunity, Innate; D007370:Interferon Type I / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D007370:Interferon Type I; D048008:TNF Receptor-Associated Factor 3; D010450:Endopeptidases; C559917:USP19 protein, human; D000072017:Deubiquitinating Enzymes
keywords: TRAF3; USP19; deubiquitinating enzyme; enterovirus 71; type I interferon signaling