title: Development of a polymorphic short tandem repeat locus multiplex system for efficient human identification.
abstract: This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the amelogenin gene locus. Strategies were developed to overcome the challenges involved in creating a multiplex system. Based on the literature and available databases, the STR loci were selected with the aim to obtain discriminatory markers, and followed specific criteria for this purpose. Primers were projected using the Primer3 software, and AutoDimer was used to evaluate possible interactions between them. The 22 selected STR loci were validated individually and jointly, both to assess their sensitivity and to test the efficiency of the multiplex system. Statistical analyses were based on the genetic data of 450 unrelated individuals living in the State of Goiás, thus allowing the establishment of the parameters necessary to use this system. A total of 239 alleles were detected for the 22 loci in the set, allowing for a probability of identity of 4.23 x 10-25to be obtained. The combined power of discrimination was 0.999999999999999999999999 and the combined power of exclusion was 0.99999. Upon complete validation of the entire system, this multiplex assay was considered to be a powerful tool for application in human identification by DNA analysis.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Rede Pró Centro-Oeste, Goiânia, GO, Brasil ricardogrodovalho@hotmail.com.|Laboratório Biocroma - Clínica de Exames de DNA, Goiânia, GO, Brasil ricardogrodovalho@hotmail.com.
authors.forename: R G
authors.initials: RG
authors.lastname: Rodovalho
authors.affiliation: Promega Corporation, Departamento de Identificação Humana, São Paulo, SP, Brasil.
authors.forename: E L
authors.initials: EL
authors.lastname: Rodrigues
authors.affiliation: Laboratório Biocroma - Clínica de Exames de DNA, Goiânia, GO, Brasil.
authors.forename: G S
authors.initials: GS
authors.lastname: Santos
authors.affiliation: Laboratório Biocroma - Clínica de Exames de DNA, Goiânia, GO, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Cavalcanti
authors.affiliation: Laboratório Biocroma - Clínica de Exames de DNA, Goiânia, GO, Brasil.
authors.forename: P R
authors.initials: PR
authors.lastname: Lima
authors.affiliation: Programa de Pos-Graduação em Gestão Organizacional - Mestrado Profissional, Universidade Federal de Goiás, Catalão, GO, Brasil.
authors.forename: A G
authors.initials: AG
authors.lastname: Rodovalho
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde, Rio Verde, GO, Brasil.
authors.forename: R G
authors.initials: RG
authors.lastname: Vital
authors.affiliation: Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Rede Pró Centro-Oeste, Goiânia, GO, Brasil.|Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Laboratório de Citogenética Humana e Biologia Molecular, Secretaria Estadual de Saúde, Goiânia, GO, Brasil.
authors.forename: M A D
authors.initials: MA
authors.lastname: Gigonzac
authors.affiliation: Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Rede Pró Centro-Oeste, Goiânia, GO, Brasil.|Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Laboratório de Citogenética Humana e Biologia Molecular, Secretaria Estadual de Saúde, Goiânia, GO, Brasil.
authors.forename: A D
authors.initials: AD
authors.lastname: da Cruz
mesh_terms: D000483:Alleles; D004247:DNA / Q000032:analysis* / Q000235:genetics; D017931:DNA Primers; D053803:Forensic Genetics / Q000379:methods*; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D018895:Microsatellite Repeats; D025202:Molecular Diagnostic Techniques / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D004247:DNA