title: An In Vitro Study of the Antioxidant and Antihemolytic Properties of Buddleja globosa (Matico).
abstract: The antioxidant and antihemolytic properties contained in the leaves of Buddleja globosa (B. globosa), also known as "Matico," were determined. Aqueous extracts of leaves were assayed in human erythrocytes and molecular models of its membrane. The latter were bilayers built-up of lipids located in the outer and inner leaflets of the erythrocyte membrane. Observations by scanning electron microscopy showed that the extract altered the morphology of erythrocytes inducing the formation of crenated echinocytes. This result implied that the extract components were inserted into the outer leaflet of the cell membrane. This conclusion was confirmed by experiments carried out by fluorescence spectroscopy of red cell membranes and vesicles (LUV) of dimyristoylphosphatidylcholine (DMPC) and by X-ray diffraction of DMPC and dimyristoylphosphatidylethanolamine bilayers. Human erythrocytes were in vitro exposed to HClO, which is a natural powerful oxidant. Results demonstrated that low concentrations of B. globosa aqueous extract neutralized the harmful capacity of HClO. Hemolysis experiments also showed that the extract in very low concentrations reduced hemolysis induced by HClO.
authors.affiliation: Faculty of Chemical Sciences, University of Concepción, Concepción, Chile. msuwalsk@udec.cl.
authors.forename: Mario
authors.initials: M
authors.lastname: Suwalsky
authors.affiliation: Centre of Biotechnology and Bioengineering, University of Chile, Santiago, Chile.
authors.forename: José
authors.initials: J
authors.lastname: Duguet
authors.affiliation: Nutrition and Food Technology Institute, University of Chile, Santiago, Chile.
authors.forename: Hernán
authors.initials: H
authors.lastname: Speisky
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D032861:Buddleja / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D004134:Dimyristoylphosphatidylcholine
keywords: Antioxidant; Buddleja globosa; Cell membrane; Human erythrocyte; Plant extract