title: Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica.
abstract: The bacterial flagellum is a motility organelle. The flagellum is composed of three main structures: the basal body as a rotary engine embedded in the cellular membranes and cell wall, the long external filament that acts as a propeller, and the hook acting as a universal joint that connects them. I describe protocols for the purification of the filament and hook-basal body from Salmonella enterica serovar Typhimurium.
authors.affiliation: Department of Life Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima, 727-0023, Japan. aizawa@pu-hiroshima.ac.jp.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Aizawa
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D064652:Basal Bodies / Q000378:metabolism*; D021961:Cell Membrane Structures / Q000378:metabolism; D005407:Flagella / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins
keywords: Basal body; Cytoplasmic membrane; Electron microscopy; Filament; Hook; Lipopolysaccharide; Peptidoglycan layer