title: Profile of Tumour Necrosis Factor Alpha -308 G/A Gene Polymorphism in Psoriatic Patients in Karnataka, India.
abstract: BACKGROUND
  Tumour necrosis factor-alpha (TNFα) gene -308G/A polymorphism (rs1800629) are associated with psoriasis in several populations worldwide. Presently, there is no literature on the status of this polymorphism in the South Indian population.
  
  
  OBJECTIVE
  To determine the profile of TNFα -308G/A polymorphism among psoriatic patients.
  
  
  METHODS
  This case-control study involved 74 patients with Psoriasis Vulgaris (PsV) and 74 age and gender matched healthy individuals. Patients were recruited from the Department of Dermatology of R.L. Jalappa Hospital and Research Center, Tamaka, Kolar, Karnataka, India, from March 2014 to March 2016. TNFα -308G/A polymorphism was genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  The frequency of TNFα -308A allele 7.4% among psoriatic and 8.8% among non-psoriatic individuals. The difference was not statistically significant (p=0.82).
  
  
  CONCLUSIONS
  Our results indicate that TNFα gene -308G/A polymorphism is not a significant marker for the risk of developing PsV among South Indian (Karnataka) psoriatic patients.
authors.affiliation: Research Assistant, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
authors.forename: Deepa
authors.initials: D
authors.lastname: Rajesh
authors.affiliation: Postgraduate Student, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
authors.forename: Chaitra
authors.initials: C
authors.lastname: Chowdappa
authors.affiliation: Assistant Professor, Department of Dermatology, Sri Devaraj Urs Medical College , Kolar, Karnataka, India .
authors.forename: Rajesh
authors.initials: R
authors.lastname: Gurumurthy
authors.affiliation: Professor, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
authors.forename: A V Moideen
authors.initials: AV
authors.lastname: Kutty
authors.affiliation: Assistant Professor, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka, India .
authors.forename: Sharath
authors.initials: S
authors.lastname: Balakrishna
keywords: Genetics; Immunology; Psoriasis vulgaris