title: Ungulate copiparvovirus 2 in healthy and postweaning multisystemic wasting syndrome-affected pigs.
abstract: A SYBR Green-based real-time polymerase chain reaction (qPCR) was designed to detect Ungulate copiparvovirus 2, also known as porcine parvovirus 4 (PPV4). The test was applied to search for PPV4 DNAemia in sera from 1- to 4-month-old pigs displaying signs of postweaning multisystemic wasting syndrome (PMWS), as well as in sera from healthy swine at equivalent age and in sera from older healthy animals (>6 months old). High levels of PPV4 DNA were detected in PMWS-affected pigs. The mean viral DNA load in PMWS-affected pigs was 5.2 × 107copies/mL, whereas in young healthy pigs it was 1.4 × 105copies/mL (P ≤ 0.001). Although the copy numbers were lower in younger PMWS-affected individuals, this result sheds some light on the possible association between PPV4 viral load detection in this group and the immune impairment caused by PMWS.
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil. spcibulski@gmail.com.
authors.forename: Samuel P
authors.initials: SP
authors.lastname: Cibulski
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Thais F
authors.initials: TF
authors.lastname: Teixeira
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Ana Paula M
authors.initials: APM
authors.lastname: Varela
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Camila M
authors.initials: CM
authors.lastname: Scheffer
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Helton F
authors.initials: HF
authors.lastname: Santos
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Francisco E S
authors.initials: FES
authors.lastname: Lima
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Paulo M
authors.initials: PM
authors.lastname: Roehe
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D019219:Parvovirinae / Q000302:isolation & purification*; D028323:Parvovirus, Porcine / Q000502:physiology; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000821:virology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D019562:Viral Load / Q000662:veterinary*
chemical_list: D004279:DNA, Viral
keywords: PPV4; Ungulate copiparvovirus 2; Viral load; qPCR