title: Expansion of highly stable blaOXA-10β-lactamase family within diverse host range among nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of Northeast India.
abstract: BACKGROUND
  The current study reports dissemination of highly stable blaOXA-10family of beta lactamases among diverse group of nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of the northern part of India.
  
  
  METHODS
  In the current study, a total number of 590 Gram negative isolates were selected for a period of 1 year (i.e. 1st November 2011-31st October 2012). Members of Enterobacteriaceae and non fermenting Gram negative rods were obtained from Silchar Medical College and Hospital, Silchar, India. Screening and molecular characterization of β-lactamase genes was done. Integrase gene PCR was performed for detection and characterization of integrons and cassette PCR was performed for study of the variable regions of integron gene cassettes carrying blaOXA-10. Gene transferability, stability and replicon typing was also carried out. Isolates were typed by ERIC as well as REP PCR.
  
  
  RESULTS
  Twenty-four isolates of Gram-negative bacilli that were harboring blaOXA-10family (OXA-14, and OXA16) with fact that resistance was to the extended cephalosporins. The resistance determinant was located within class I integron in five diverse genetic contexts and horizontally transferable in Enterobacteriaceae, was carried through IncY type plasmid. MIC values were above break point for all the tested cephalosporins. Furthermore, co-carriage of blaCMY-2was also observed.
  
  
  CONCLUSIONS
  Multiple genetic environment of blaOXA-10in this geographical region must be investigated to prevent dissemination of these gene cassettes within bacterial population within hospital settings.
authors.affiliation: Department of Microbiology, Assam University, Silchar, 788011, India.
authors.forename: Anand Prakash
authors.initials: AP
authors.lastname: Maurya
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, 788014, India.
authors.forename: Debadatta
authors.initials: D
authors.lastname: Dhar
authors.affiliation: Department of Microbiology, Assam University, Silchar, 788011, India.
authors.forename: Mridul Kumar
authors.initials: MK
authors.lastname: Basumatary
authors.affiliation: Department of Microbiology, Assam University, Silchar, 788011, India.
authors.forename: Deepjyoti
authors.initials: D
authors.lastname: Paul
authors.affiliation: Department of Microbiology, Assam University, Silchar, 788011, India.
authors.forename: Birson
authors.initials: B
authors.lastname: Ingti
authors.affiliation: Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, India.
authors.forename: Debarati
authors.initials: D
authors.lastname: Choudhury
authors.affiliation: Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, India.
authors.forename: Anupam Das
authors.initials: AD
authors.lastname: Talukdar
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, 788014, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Chakravarty
authors.affiliation: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Microbiology, Assam University, Silchar, 788011, India. ab0404@gmail.com.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Bhattacharjee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D003428:Cross Infection / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005260:Female; D058507:Host Specificity; D006801:Humans; D007194:India; D032023:Integrons / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D005810:Multigene Family; D013045:Species Specificity; D062606:Tertiary Care Centers; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C425107:beta-lactamase OXA-10; C096781:beta-lactamase OXA-14; C117195:beta-lactamase OXA-16; D001618:beta-Lactamases
keywords: Expanded-spectrum cephalosporins; Gram-negative rods; IncY; Integron; OXA-10 family