title: Color Representation Is Retinotopically Biased but Locally Intermingled in Mouse V1.
abstract: Dichromatic vision is common in many mammals. However, color processing in the primary visual cortex (V1) of dichromatic mammals is relatively unknown compared to the trichromatic primates. In this study, we investigated the functional organization of color processing in mouse V1. The mouse retina has a graded expression pattern of two opsins along its dorsoventral axis. However, it is not clear whether and how this expression pattern is reflected in the cortical representation at local (several hundred microns) and areal (V1) level. Usingin vivotwo-photon calcium (Ca2+) imaging and wide-field Ca2+imaging, we revealed that V1 neurons responded to S (UV)- and M (green)-opsin isolating stimuli with slightly biased color preference depending on retinotopic position in V1. This was consistent with the distribution of retinal opsins. At the cellular level, preferences for S- and M-opsin isolating stimuli were intermingled in a local region encompassing several hundred microns. These results suggest that functional organizations of color information are locally intermingled, but slightly biased depending on the retinotopic position in mouse V1.
authors.affiliation: Department of Molecular Physiology, Graduate School of Medical Sciences, Kyushu University Fukuoka, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Aihara
authors.affiliation: Department of Molecular Physiology, Graduate School of Medical Sciences, Kyushu UniversityFukuoka, Japan; Department of Physiology, The University of Tokyo School of MedicineTokyo, Japan; Core Research for Evolutionary Science and Technology (CREST), Japan Science and Technology AgencyTokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Department of Molecular Physiology, Graduate School of Medical Sciences, Kyushu University Fukuoka, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Department of Molecular Physiology, Graduate School of Medical Sciences, Kyushu UniversityFukuoka, Japan; Department of Physiology, The University of Tokyo School of MedicineTokyo, Japan; Core Research for Evolutionary Science and Technology (CREST), Japan Science and Technology AgencyTokyo, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ohki
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D003118:Color Perception / Q000502:physiology*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D061848:Optical Imaging; D010775:Photic Stimulation; D014793:Visual Cortex / Q000166:cytology / Q000502:physiology*
chemical_list: D002413:Cations, Divalent; D002118:Calcium
keywords: color; functional architecture; mouse; two-photon imaging; visual cortex; wide field Ca2+ imaging