title: Unr defines a novel class of nucleoplasmic reticulum involved in mRNA translation.
abstract: Unr (officially known as CSDE1) is a cytoplasmic RNA-binding protein with roles in the regulation of mRNA stability and translation. In this study, we identified a novel function for Unr, which acts as a positive regulator of placental development. Unr expression studies in the developing placenta revealed the presence of Unr-rich foci that are apparently located in the nuclei of trophoblast giant cells (TGCs). We determined that what we initially thought to be foci, were actually cross sections of a network of double-wall nuclear membrane invaginations that contain a cytoplasmic core related to the nucleoplasmic reticulum (NR). We named them, accordingly, Unr-NRs. Unr-NRs constitute a novel type of NR because they contain high levels of poly(A) RNA and translation factors, and are sites of active translation. In murine tissues, Unr-NRs are only found in two polyploid cell types, in TGCs and hepatocytes.In vitro, their formation is linked to stress and polyploidy because, in three cancer cell lines, cytotoxic drugs that are known to promote polyploidization induce their formation. Finally, we show that Unr is requiredin vivofor the formation of Unr-containing NRs because these structures are absent inUnr-null TGCs.
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France frederic.saltel@inserm.fr helene.jacquemin-sablon@u-bordeaux.fr.|University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Frédéric
authors.identifier: 0000-0002-0724-9680
authors.initials: F
authors.lastname: Saltel
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France.|University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Alban
authors.initials: A
authors.lastname: Giese
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France.|University of Bordeaux, F-33000 Bordeaux, France.|Department of Tumor Biology, CHU, F-33000 Bordeaux, France.
authors.forename: Lamia
authors.initials: L
authors.lastname: Azzi
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France.|University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Habiba
authors.initials: H
authors.lastname: Elatmani
authors.affiliation: Laboratoire de Transgenèse, Université Bordeaux, F-33000 Bordeaux, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Costet
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France.|University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Zakaria
authors.initials: Z
authors.lastname: Ezzoukhry
authors.affiliation: University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Dugot-Senant
authors.affiliation: Aix-Marseille University, CNRS, IBDM UMR 7288, Marseille, France.
authors.forename: Lucile
authors.initials: L
authors.lastname: Miquerol
authors.affiliation: Taconic Europe, Centre d'Affaires DMCI, Lyon, France.
authors.forename: Oréda
authors.initials: O
authors.lastname: Boussadia
authors.affiliation: University of Bordeaux, F-33000 Bordeaux, France.|MFP CNRS UMR 5234, Microbiologie Fondamentale et Pathogénicité, Université de Bordeaux, F-33000 Bordeaux, France.
authors.forename: Harald
authors.initials: H
authors.lastname: Wodrich
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France.|University of Bordeaux, F-33000 Bordeaux, France.|Department of Tumor Biology, CHU, F-33000 Bordeaux, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Dubus
authors.affiliation: INSERM UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, F-33000 Bordeaux, France frederic.saltel@inserm.fr helene.jacquemin-sablon@u-bordeaux.fr.|University of Bordeaux, F-33000 Bordeaux, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Jacquemin-Sablon
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D020964:Embryo Loss / Q000473:pathology; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D008810:Mice, Inbred C57BL; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D010920:Placenta / Q000002:abnormalities; D011061:Poly A; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011123:Polyploidy; D011247:Pregnancy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013312:Stress, Physiological; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D039642:Eukaryotic Initiation Factors; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; C489441:UNR protein, mouse; D011061:Poly A
keywords: CSDE1; Nucleoplasmic reticulum; Polyploid cells; Unr; Unr knockout; mRNA translation