title: Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MSnwith the Aid of Chemometrics.
abstract: Ganodermatriterpenes (GTs) are the major secondary metabolites ofGanoderma lucidum, which is a popularly used traditional Chinese medicine for complementary cancer therapy. The present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Cluster Analysis (CA) and Principal Component Analysis (PCA) for the identification and quality control ofG. lucidum. Fifteen samples from the Chinese provinces of Hainan, Neimeng, Shangdong, Jilin, Anhui, Henan, Yunnan, Guangxi and Fujian were analyzed by HPLC-PAD and HPLC-MSn. Forty-seven compounds were detected by HPLC, of which forty-two compounds were tentatively identified by comparing their retention times and mass spectrometry data with that of reference compounds and reviewing the literature. Ganoderic acid B, 3,7,15-trihydroxy-11,23-dioxolanost-8,16-dien-26-oic acid, lucidenic acid A, ganoderic acid G, and 3,7-oxo-12-acetylganoderic acid DM were deemed to be the marker compounds to distinguish the samples with different quality according to both CA and PCA. This study provides helpful chemical information for further research on the anti-tumor activity and mechanism of action ofG. lucidum. The results proved that fingerprints combined with chemometrics are a simple, rapid and effective method for the quality control ofG. lucidum.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. fanglingwu@163.com.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Wu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. lwy1054289310@163.com.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Liang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. sdcwjing@163.com.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. lishi816@126.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. 20150931830@bucm.edu.cn.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Cui
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. cici_jiayou@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zhanglanzhen01@126.com.
authors.forename: Lanzhen
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D013058:Mass Spectrometry; D015394:Molecular Structure; D025341:Principal Component Analysis; D020940:Reishi / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D001688:Biological Products
keywords: Ganoderma lucidum; HPLC-MSn; Similarity Analysis (SA); chemometrics; triterpenes