title: The Non-Peptide Vasopressin V1b Receptor Antagonist, SSR149415, Ameliorates Spermatogenesis Function in a Mouse Model of Chronic Social Defeat Stress.
abstract: To determine the effects of SSR149415 on testis and spermatogenesis in male mice subjected to chronic social defeat stress, C57BL/6 male mice were divided into two groups: Control and Stress. Then Stress group was subdivided into four subgroups administered water, SSR149415 (1 mg/kg/day), SSR149415 (10 mg/kg/day), SSR149415 (30 mg/kg/day), respectively. The behavioral alterations revealed by social interaction test and open field test were measured. The physical indices, including body weight and gonad weight (testis and epididymis) as well as testis/body weight and cauda epididymis/body weight were detected. Serum hormones, including testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were determined. Sperm count and abnormality as well as testicular histology structure were assessed. The germ cells apoptosis were also evaluated. Chronic social defeat stress-induced behavioral abnormality, as well as gonad atrophy (testis and epididymis) was significantly alleviated in stressed male mice exposed to SSR149415. Regressed serum testosterone levels and elevated serum FSH and LH levels exhibited by stressed male mice were observably reversed following SSR149415 administration. Chronic social defeat stress-induced damage in testicular histology structure and semen quality were also improved after SSR149415 administration. In addition, SSR149415 significantly reversed chronic social defeat stress-induced germ cells apoptosis. Overall, we provide clear evidence indicating the amelioration of chronic social defeat stress-induced behavioral abnormality and testicular dysfunction via SSR149415, promoting the development of drug-directed therapy against this disease. J. Cell. Biochem. 118: 3891-3898, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Urology, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Zhuang
authors.affiliation: Department of Urology, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Liang-Liang
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Urology, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Jin-Xian
authors.initials: JX
authors.lastname: Pu
authors.affiliation: Department of Urology, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xia
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of SooChow University, SuZhou, 215006, China.
authors.forename: Jin-Xing
authors.identifier: 0000-0002-5185-1441
authors.initials: JX
authors.lastname: Lv
mesh_terms: D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists / Q000494:pharmacology*; D002908:Chronic Disease; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011759:Pyrrolidines / Q000494:pharmacology*; D017483:Receptors, Vasopressin / Q000378:metabolism*; D012919:Social Behavior; D013091:Spermatogenesis / Q000187:drug effects*; D013315:Stress, Psychological / Q000378:metabolism* / Q000473:pathology
chemical_list: C456029:1-(5-chloro-1-((2,4-dimethoxyphenyl)sulfonyl)-3-(2-methoxyphenyl)-2-oxo-2,3-dihydro-1H-indol-3-yl)-4-hydroxy-N,N-dimethyl-2-pyrrolidinecarboxamide; D065092:Antidiuretic Hormone Receptor Antagonists; D007211:Indoles; D011759:Pyrrolidines; D017483:Receptors, Vasopressin
keywords: CHRONIC SOCIAL DEFEAT STRESS; SPERMATOGENESIS; SSR149415; TESTIS ATROPHY