title: Molecular Characterization of Hypoderma SPP. in Domestic Ruminants from Turkey and Pakistan.
abstract: The aim of this study was to determine the morphological and molecular characterization of Hypoderma spp. in cattle and yak from provinces in Turkey and Pakistan. In total, 78 Hypoderma larvae were collected from slaughtered animals in Turkey and Pakistan from October 2015 to January 2016. Thirty-eight of these 78 Hypoderma larvae were morphologically classified as third instar larvae (L3s) of Hypoderma bovis, 37 were classified as Hypoderma lineatum, and 3 were classified as suspected or unidentified. The restriction enzyme TaqI was used to differentiate the Hypoderma spp. by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). According to the sequences and the PCR-RFLP results, all larval samples from cattle from Turkey were classified as H. bovis, except for 1 sample classified as H. lineatum. All Hypoderma larvae from Pakistan were classified as H. lineatum from cattle and as Hypoderma sinense from yak. This study provides the first molecular characterization of H. lineatum (cattle) and H. sinense (yak) in Pakistan based on PCR-RFLP and sequencing results.
authors.affiliation: *   Department of Bio Sciences, COMSATS Institute of Information Technology (CIIT), Park Road, Chakh Shazad, Islamabad 45550, Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Ahmed
authors.forename: Sami
authors.initials: S
authors.lastname: Simsek
authors.forename: Cem Ecmel
authors.initials: CE
authors.lastname: Saki
authors.forename: Harun Kaya
authors.initials: HK
authors.lastname: Kesik
authors.forename: Seyma Gunyakti
authors.initials: SG
authors.lastname: Kilinc
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004175:Diptera / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D007814:Larva / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics; D008297:Male; D009198:Myiasis / Q000469:parasitology / Q000662:veterinary*; D010154:Pakistan; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D014421:Turkey
chemical_list: D004247:DNA; D003576:Electron Transport Complex IV