title: Syrian hamster neuroplasticity mechanisms fail as temperature declines to 15 °C, but histaminergic neuromodulation persists.
abstract: Previous research suggests that hippocampal neurons in mammalian hibernators shift their major function from memory formation at euthermic brain temperatures (Tb= ~37 °C) to modulation of hibernation bout duration as Tbdecreases. This role of hippocampal neurons during torpor is based in part on in vivo studies showing that histamine (HA) infused into ground squirrel hippocampi lengthened torpor bouts by ~50%. However, it was unclear if HA acted directly on hippocampal neurons or on downstream brain regions via HA spillover into lateral ventricles. To clarify this, we used hippocampal slices to determine if HA would modulate pyramidal neurons at low levels of synaptic activity (as occurs in torpor). We tested the hypotheses that although LTP (a neuroplasticity mechanism) could not be generated at low temperatures, HA (via H2 receptors) would increase population spike amplitudes (PSAs) of Syrian hamster CA1 pyramidal neurons at low stimulation voltages and low temperatures. PSAs were recorded following Schaffer collateral stimulation from subthreshold levels to a maximum response plateau. We found that tetanus evoked LTP at 35 °C but not 15 °C; and at temperatures from 30 to 15 °C, HA significantly enhanced PSA at near threshold levels in slices from non-hibernating hamsters housed in "summer-like" or "winter-like" conditions and from hibernating hamsters. Cimetidine (H2 antagonist) blocked HA-mediated PSA increases in 8 of 8 slices; pyrilamine (H1 antagonist) had no effect in 7 of 8 slices. These results support our hypotheses and show that HA can directly enhance pyramidal neuron excitability via H2 receptors and thus may prolong torpor bouts.
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California at Davis, One Shields Ave, Davis, CA, 95616, USA.
authors.forename: Jock S
authors.initials: JS
authors.lastname: Hamilton
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California at Davis, One Shields Ave, Davis, CA, 95616, USA.
authors.forename: Sat M
authors.initials: SM
authors.lastname: Chau
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California at Davis, One Shields Ave, Davis, CA, 95616, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Malins
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California at Davis, One Shields Ave, Davis, CA, 95616, USA.
authors.forename: Giancarlo G
authors.initials: GG
authors.lastname: Ibanez
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California at Davis, One Shields Ave, Davis, CA, 95616, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Horowitz
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California at Davis, One Shields Ave, Davis, CA, 95616, USA. bahorwitz@ucdavis.edu.
authors.forename: Barbara A
authors.identifier: http://orcid.org/0000-0002-0345-8717
authors.initials: BA
authors.lastname: Horwitz
mesh_terms: D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D017774:Long-Term Potentiation; D008647:Mesocricetus / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D017966:Pyramidal Cells / Q000502:physiology*; D011970:Receptors, Histamine H2 / Q000502:physiology*; D013696:Temperature; D064348:Torpor / Q000502:physiology*
chemical_list: D006635:Histamine H2 Antagonists; D011970:Receptors, Histamine H2; D002927:Cimetidine; D006632:Histamine
keywords: Ascending arousal system; CA1 pyramidal cells; Hibernation; Histamine; Torpor