title: Microbial fuel cell characterisation and evaluation of Lysinibacillus macroides MFC02 electrigenic capability.
abstract: Microbial fuel cell (MFC) is the most prominent research field due to its capability to generate electricity by utilizing the renewable sources. In the present study, Two MFC designs namely, H type-Microbial fuel cell (HT-MFC) and U type-Microbial fuel cell (UT-MFC) were constructed based on standardized H shaped anode and cathode compartment as well as U shaped anode and cathode compartments, respectively. In order to lower the cost for MFC construction, Pencil graphite lead was used as electrode and salt agar as Proton exchange membrane. Results inferred that newly constructed UT-MFC showed high electron production when compared to the HT-MFC. UT-MFC displayed an output of about 377 ± 18.85 mV (millivolts); whereas HT-MFC rendered only 237 ± 11.85 mV (millivolts) of power generation, which might be due to the low internal resistance. By increasing the number of cathode in UT-MFC, power production was increased upto 313 ± 15.65 mV in Open circuit voltage (OCV). Electrogenic bacteria namely, Lysinibacillus macroides (Acc. No. KX011879) rendered enriched power generation. The attachment of bacteria as a biofilm on pencil graphite lead was analyzed using fluorescent microscope and Scanning Electron Microscope (SEM). Based on our findings, it was observed that UT-MFC has a tendency to produce high electron generation using pencil graphite lead as the electrode material.
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.
authors.forename: Murugan
authors.initials: M
authors.lastname: Uma Vanitha
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Natarajan
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.
authors.forename: Harikrishnamoorthy
authors.initials: H
authors.lastname: Sridhar
authors.affiliation: Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India. umamsu@gmail.com.
authors.forename: Sankaran
authors.identifier: http://orcid.org/0000-0002-5910-5881
authors.initials: S
authors.lastname: Umamaheswari
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D010802:Phylogeny
chemical_list: D006108:Graphite
keywords: Lysinibacillus macroides MFC02; Microbial fuel cell power generation; Pencil graphite lead