title: Comparison of Mitochondrial Function in Boar and Bull Spermatozoa Throughout Cryopreservation Based on JC-1 Staining.
abstract: BACKGROUND
  Poor reproductivity hampers the commercialization of cryopreserved boar semen.
  
  
  OBJECTIVE
  This study was to determine the differences in the sperm mitochondrial function between boar and bull semen at different cryopreservation stages.
  
  
  METHODS
  Boar and bull fresh, equilibrated, and frozen-thawed spermatozoa were evaluated for mitochondrial function using JC-1 under a fluorescent microscope.
  
  
  RESULTS
  Bull and boar percentage of spermatozoa staining green (PSSG) showed no difference between fresh and equilibrated semen (P> 0.05). However, frozen-thawed bull and boar semen demonstrated significantly higher PSSG (P < 0.01) than fresh and equilibrated semen. Frozen-thawed boar semen represented a significantly higher PSSG (P < 0.01) than bull semen.
  
  
  CONCLUSIONS
  Negative cryopreservation influence on boar and bull spermatozoa was not significantly produced by pre-freezing procedures, but rather by freezing and thawing. Cryopreservation has more pronounced negative effects on boar than on bull spermatozoa, which partly explains lagged commercialization of frozen boar semen.
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhuang
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Wei
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: S S
authors.initials: SS
authors.lastname: Lu
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Lu
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning, China. kehuanlu@163.com.
authors.forename: K H
authors.initials: KH
authors.lastname: Lu
mesh_terms: D000818:Animals; D001562:Benzimidazoles; D002232:Carbocyanines; D002417:Cattle; D015925:Cryopreservation / Q000379:methods; D005456:Fluorescent Dyes; D005615:Freezing; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D012661:Semen / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods; D013094:Spermatozoa / Q000378:metabolism*; D013194:Staining and Labeling; D013552:Swine
chemical_list: D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine