title: Modified n-HA/PA66 scaffolds with chitosan coating for bone tissue engineering: cell stimulation and drug release.
abstract: The dipping-drying procedure and cross-linking method were used to make drug-loaded chitosan (CS) coating on nano-hydroxyapatite/polyamide66 (nHA/PA66) composite porous scaffold, endowing the scaffold controlled drug release functionality. The prefabricated scaffold was immersed into an aqueous drug/CS solution in a vacuum condition and then crosslinked by vanillin. The structure, porosity, composition, compressive strength, swelling ratio, drug release and cytocompatibility of the pristine and coating scaffolds were investigated. After coating, the scaffold porosity and pore interconnection were slightly decreased. Cytocompatibility performance was observed through an in vitro experiment based on cell attachment and the MTT assay by MG63 cells which revealed positive cell viability and increasing proliferation over the 11-day period in vitro. The drug could effectively release from the coated scaffold in a controlled fashion and the release rate was sustained for a long period and highly dependent on coating swelling, suggesting the possibility of a controlled drug release. Our results demonstrate that the scaffold with drug-loaded crosslinked CS coating can be used as a simple technique to render the surfaces of synthetic scaffolds active, thus enabling them to be a promising high performance biomaterial in bone tissue engineering.
authors.affiliation: a Research Center for Nano-Biomaterials, Analytical & Testing Center , Sichuan University , Chengdu , China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zou
authors.affiliation: b Department of Materials Science & Engineering , Chengdu University of Technology , Chengdu , China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: a Research Center for Nano-Biomaterials, Analytical & Testing Center , Sichuan University , Chengdu , China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Niu
authors.affiliation: a Research Center for Nano-Biomaterials, Analytical & Testing Center , Sichuan University , Chengdu , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: a Research Center for Nano-Biomaterials, Analytical & Testing Center , Sichuan University , Chengdu , China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Li
authors.affiliation: a Research Center for Nano-Biomaterials, Analytical & Testing Center , Sichuan University , Chengdu , China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D065546:Drug Liberation; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D009757:Nylons; D048271:Chitosan; D017886:Durapatite
keywords: Porous composite scaffold; chitosan coating; control release; crosslinking; cytocompatibility