title: Resveratrol induces mitochondrial dysfunction and decreases chronological life span of Saccharomyces cerevisiae in a glucose-dependent manner.
abstract: A broad range of health benefits have been attributed to resveratrol (RSV) supplementation in mammalian systems, including the increases in longevity. Nonetheless, despite the growing number of studies performed with RSV, the molecular mechanism by which it acts still remains unknown. Recently, it has been proposed that inhibition of the oxidative phosphorylation activity is the principal mechanism of RSV action. This mechanism suggests that RSV might induce mitochondrial dysfunction resulting in oxidative damage to cells with a concomitant decrease of cell viability and cellular life span. To prove this hypothesis, the chronological life span (CLS) of Saccharomyces cerevisiae was studied as it is accepted as an important model of oxidative damage and aging. In addition, oxygen consumption, mitochondrial membrane potential, and hydrogen peroxide (H2O2) release were measured in order to determine the extent of mitochondrial dysfunction. The results demonstrated that the supplementation of S. cerevisiae cultures with 100 μM RSV decreased CLS in a glucose-dependent manner. At high-level glucose, RSV supplementation increased oxygen consumption during the exponential phase yeast cultures, but inhibited it in chronologically aged yeast cultures. However, at low-level glucose, oxygen consumption was inhibited in yeast cultures in the exponential phase as well as in chronologically aged cultures. Furthermore, RSV supplementation promoted the polarization of the mitochondrial membrane in both cultures. Finally, RSV decreased the release of H2O2with high-level glucose and increased it at low-level glucose. Altogether, this data supports the hypothesis that RSV supplementation decreases CLS as a result of mitochondrial dysfunction and this phenotype occurs in a glucose-dependent manner.
authors.affiliation: Universidad Autónoma de Querétaro, Cerro de las Campanas, 76010, Santiago de Querétaro, Qro, Mexico.
authors.forename: Minerva
authors.initials: M
authors.lastname: Ramos-Gomez
authors.affiliation: Universidad Autónoma de Querétaro, Cerro de las Campanas, 76010, Santiago de Querétaro, Qro, Mexico.
authors.forename: Ivanna Karina
authors.initials: IK
authors.lastname: Olivares-Marin
authors.affiliation: Laboratorio de Biotecnología Microbiana del Instituto Tecnológico Superior de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez #2120, 61100, Ciudad Hidalgo, Michoacán, Mexico.
authors.forename: Melina
authors.initials: M
authors.lastname: Canizal-García
authors.affiliation: Laboratorio de Bioquímica del Instituto Tecnológico de Morelia, Av. Tecnológico de Morelia #1500, 58120, Morelia, Michoacán, Mexico.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: González-Hernández
authors.affiliation: Universidad Autónoma de Querétaro, Cerro de las Campanas, 76010, Santiago de Querétaro, Qro, Mexico.
authors.forename: Gerardo M
authors.initials: GM
authors.lastname: Nava
authors.affiliation: Universidad Autónoma de Querétaro, Cerro de las Campanas, 76010, Santiago de Querétaro, Qro, Mexico. lmadrigal@itsch.edu.mx.|Laboratorio de Biotecnología Microbiana del Instituto Tecnológico Superior de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez #2120, 61100, Ciudad Hidalgo, Michoacán, Mexico. lmadrigal@itsch.edu.mx.
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Madrigal-Perez
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008136:Longevity / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D006861:Hydrogen Peroxide; D005947:Glucose; D000077185:Resveratrol
keywords: Aging; Glucose; Mitochondrial dysfunction; Resveratrol