title: Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum).
abstract: The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE)in vitroandin vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-α production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNγ.In vivostudies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement.
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project Team, Chonnam National University, Gwangju, Korea.
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project Team, Chonnam National University, Gwangju, Korea.
authors.forename: Sung-Gang
authors.initials: SG
authors.lastname: Jo
authors.affiliation: Institute of Biotechnology, Bioresource Inc., Cheomdan venture, Gwangju, Korea.
authors.forename: Seoung-Ki
authors.initials: SK
authors.lastname: Jung
authors.affiliation: Institute of Biotechnology, Bioresource Inc., Cheomdan venture, Gwangju, Korea.
authors.forename: Woo-Tae
authors.initials: WT
authors.lastname: Park
authors.affiliation: Institute of Biotechnology, Bioresource Inc., Cheomdan venture, Gwangju, Korea.
authors.forename: Keun-Young
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Department of Rheumatology, Chonnam National University Medical School, Chonnam National University Hospital, Gwangju, Korea.
authors.forename: Yong-Wook
authors.initials: YW
authors.lastname: Park
authors.affiliation: Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project Team, Chonnam National University, Gwangju, Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
keywords: Ice plant extract (IPE); cell proliferation; cytokines; lymphocyte; macrophage