title: Development and validation of a solid-phase extraction method coupled with HPLC-UV detection for the determination of biogenic amines in Chinese rice wine.
abstract: A reliable and accurate method for the determination of seven biogenic amines (BAs) was developed and validated with Chinese rice wine samples. The BAs were derivatised with dansyl chloride, cleaned up using solid-phase extraction (SPE) and separated by high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection. The optimised derivatisation reaction, conducted at pH 9.6 and 60°C for 30 min, ensured baseline separation and peak symmetry for each BA. SPE clean-up using Oasis MCX cartridges yielded good recovery rates for all BAs and effectively reduced matrix effects. The developed method shows good linearity with determination coefficients of more than 0.9989 over a concentration range of 0.1-100 mg l-1. The limits of detection (LODs) for the investigated BAs ranged from 2.07 to 5.56 µg l-1. The intra- and inter-day relative standard deviations (RSDs) ranged from 0.86% to 3.81% and from 2.13% to 3.82%, respectively. Spiking experiments showed that the overall recovery rates ranged from 85% to 113%. Thus, the proposed method was demonstrated as being suitable for simultaneous detection, with accurate and precise quantification, of BAs in Chinese rice wine.
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: b Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhu
authors.affiliation: c College of Chemical Engineering and Materials Science , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: b Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Hao-Yu
authors.initials: HY
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Lv
authors.affiliation: b Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology , Tianjin University of Science and Technology , Tianjin , China.|d Tianjin SF-Bio Industrial Bio-Tec Co., Ltd , Tianjin , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001679:Biogenic Amines / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid; D012275:Oryza / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D014466:Ultraviolet Rays; D014920:Wine / Q000032:analysis*
chemical_list: D001679:Biogenic Amines
keywords: Biogenic amines; Chinese rice wine; high-performance liquid chromatography; matrix effects; solid-phase extraction