title: Matrix Metalloproteinase-9 rs17576 Gene Polymorphism and Behçet's Disease: Is There an Association?
abstract: BACKGROUND
  Clinical studies have reported a significant association between matrix metalloproteinases (MMP), particularly (MMP-9), and inflammatory diseases including Behçet's disease (BD).
  
  
  OBJECTIVE
  To study the relationship between MMP-9 rs17576 gene polymorphism and the development of BD, and its relation to disease activity among Egyptian patients.
  
  
  METHODS
  A total of 100 BD patients and 100 healthy control volunteers were genotyped for MMP-9 rs17576 polymorphism with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), followed by the confirmation of our results in random subgroups using direct DNA sequencing technique.
  
  
  RESULTS
  The frequency of the GG genotype and G allele was significantly higher in BD patients as compared to the normal controls (p = 0.011, OR 8.61; p = 0.03, OR 1.65, respectively). There was no significant association between the MMP-9 rs17576 polymorphism and the clinical outcomes of BD.
  
  
  CONCLUSIONS
  our study suggests a significant association of the MMP-9 rs17576 A/G polymorphism with increased risk of BD development in Egyptian patients.
authors.affiliation: a Department of Clinical & Chemical Pathology, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors.forename: Asmaa
authors.initials: A
authors.lastname: Kamal
authors.affiliation: b Department of Rheumatology and Rehabilitation, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors.forename: Fatema T
authors.initials: FT
authors.lastname: Elgengehy
authors.affiliation: c Internal Medicine Department, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors.forename: Mohamed Momtaz
authors.initials: MM
authors.lastname: Abd Elaziz
authors.affiliation: b Department of Rheumatology and Rehabilitation, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors.forename: Sherif M
authors.initials: SM
authors.lastname: Gamal
authors.affiliation: b Department of Rheumatology and Rehabilitation, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors.forename: Nesreen
authors.initials: N
authors.lastname: Sobhy
authors.affiliation: d Complementary Medicine Department , National Research Center , Cairo , Egypt.
authors.forename: Amira
authors.initials: A
authors.lastname: Medhat
authors.affiliation: b Department of Rheumatology and Rehabilitation, Faculty of Medicine , Cairo University , Cairo , Egypt.
authors.forename: Al Hussein M
authors.initials: AHM
authors.lastname: El Dakrony
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence; D001528:Behcet Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D004534:Egypt; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012306:Risk; D017422:Sequence Analysis, DNA
chemical_list: C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Behçet’s disease; MMP-9 rs17576; gene polymorphism; matrix metalloproteinase