title: Analysis of Differentially Expressed Genes in Gastrocnemius Muscle between DGAT1 Transgenic Mice and Wild-Type Mice.
abstract: Adipose tissue was the major energy deposition site of the mammals and provided the energy for the body and released the external pressure to the internal organs. In animal production, fat deposition in muscle can affect the meat quality, especially the intramuscular fat (IMF) content. Diacylglycerol acyltransferase-1 (DGAT1) was the key enzyme to control the synthesis of the triacylglycerol in adipose tissue. In order to better understand the regulation mechanism of the DGAT1 in the intramuscular fat deposition, the global gene expression profiling was performed in gastrocnemius muscle between DGAT1 transgenic mice and wild-type mice by microarray. 281 differentially expressed transcripts were identified with at least 1.5-fold change and thepvalue < 0.05. 169 transcripts were upregulated and 112 transcripts were downregulated. Ten genes (SREBF1, DUSP1, PLAGL1, FKBP5, ZBTB16, PPP1R3C, CDC14A, GLUL, PDK4, and UCP3) were selected to validate the reliability of the chip's results by the real-time PCR. The finding of RT-PCR was consistent with the gene chip. Seventeen signal pathways were analyzed using KEGG pathway database and the pathways concentrated mainly on the G-protein coupled receptor protein signaling pathway, signal transduction, oxidation-reduction reaction, olfactory receptor activity, protein binding, and zinc ion binding. This study implied a function role of DGAT1 in the synthesis of TAG, insulin resistance, and IMF deposition.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Fei
authors.identifier: 0000-0002-1381-3882
authors.initials: F
authors.lastname: Ying
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hao
authors.identifier: 0000-0002-2798-6522
authors.initials: H
authors.lastname: Gu
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors.forename: Bo
authors.identifier: 0000-0001-5653-0797
authors.initials: B
authors.lastname: Zuo
mesh_terms: D000273:Adipose Tissue / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D051048:Diacylglycerol O-Acyltransferase / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D007333:Insulin Resistance / Q000235:genetics; D008460:Meat; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction; D014280:Triglycerides / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D014280:Triglycerides; C494882:Dgat1 protein, mouse; D051048:Diacylglycerol O-Acyltransferase