title: Sharp wave ripples during learning stabilize the hippocampal spatial map.
abstract: Cognitive representation of the environment requires a stable hippocampal map, but the mechanisms maintaining a given map are unknown. Because sharp wave-ripples (SPW-R) orchestrate both retrospective and prospective spatial information, we hypothesized that disrupting neuronal activity during SPW-Rs affects spatial representation. Mice learned new sets of three goal locations daily in a multiwell maze. We used closed-loop SPW-R detection at goal locations to trigger optogenetic silencing of a subset of CA1 pyramidal neurons. Control place cells (nonsilenced or silenced outside SPW-Rs) largely maintained the location of their place fields after learning and showed increased spatial information content. In contrast, the place fields of SPW-R-silenced place cells remapped, and their spatial information remained unaltered. SPW-R silencing did not impact the firing rates or proportions of place cells. These results suggest that interference with SPW-R-associated activity during learning prevents stabilization and refinement of hippocampal maps.
authors.affiliation: New York University Neuroscience Institute, New York University, New York, New York, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Roux
authors.affiliation: New York University Neuroscience Institute, New York University, New York, New York, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.affiliation: New York University Neuroscience Institute, New York University, New York, New York, USA.
authors.forename: Ronny
authors.initials: R
authors.lastname: Eichler
authors.affiliation: New York University Neuroscience Institute, New York University, New York, New York, USA.|Tel Aviv University, Sackler Faculty of Medicine and Sagol School of Neuroscience, Department of Physiology and Pharmacology, Tel Aviv, Israel.
authors.forename: Eran
authors.initials: E
authors.lastname: Stark
authors.affiliation: New York University Neuroscience Institute, New York University, New York, New York, USA.|Department of Neurology, Medical Center, New York University, New York, New York, USA.|Center for Neural Science, New York University, New York, New York, USA.
authors.forename: György
authors.initials: G
authors.lastname: Buzsáki
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000502:physiology*; D004558:Electric Stimulation; D007858:Learning / Q000502:physiology*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009433:Neural Inhibition / Q000502:physiology*; D000071037:Place Cells / Q000502:physiology*; D017966:Pyramidal Cells / Q000502:physiology*; D012890:Sleep; D065852:Spatial Memory / Q000502:physiology*