title: Identification of a novel Hemoplasma species from pigs in Zhejiang province, China.
abstract: Hemoplasmas belong to Mycoplasmataceae (Mollicutes: Mycoplasmatales) and are able to infect a broad range of mammalian species. We investigated prevalence of hemotropic mycoplasma species in pig farms in the region of Zhejiang by a PCR scheme using universal primers targeting 16S rRNA and RNase P RNA gene (rnpB). Representative positive samples from different farms were selected for sequencing of 16S rRNA and the 219bp rnpB gene fragments for phylogenetic analysis. Sequencing analysis of PCR products from first samples identified a novel hemoplasma species present in several pig farms in the region with highest nucleotide identity of 92% to Candidatus Mycoplasma turicensis. A duplex PCR assay was then designed for differential detection of the novel hemoplasma from Mycoplasma parvum/M. suis in field samples. Of 324 blood samples from clinically healthy pigs, 26.5% was positive for this novel hemoplasma species and 50% positive for M. suis/M. parvum, indicating that the novel hemotropic mycoplasma species were of considerably high prevalence in Zhejiang province, China.
authors.affiliation: Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.|Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Tuanyuan
authors.initials: T
authors.lastname: Shi
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xu
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.|College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Fuzhuang
authors.initials: F
authors.lastname: Lu
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Junxing
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Lv
authors.affiliation: Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Weihuan
authors.initials: W
authors.lastname: Fang
mesh_terms: D000818:Animals; D002681:China; D009178:Mycoplasmataceae / Q000145:classification / Q000302:isolation & purification*; D009180:Mycoplasmatales Infections / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Mycoplasma parvum; Mycoplasma suis; hemoplasma; novel hemotropic Mycoplasma hemosuis