title: Immunoproteomics Approach for Screening of Vaccine Candidates against Intestinal Botulism.
abstract: Intestinal botulism is an infectious form of botulism in which disease results from ingesting spores, which is followed by spore germination and intraluminal production of botulinum neurotoxins over an extended period. Botulinum neurotoxin is produced by endospore forming bacteria called C. botulinum. Immunoproteomic study was used to screen the cross reactive immunogenic proteins of Clostridium botulinum type B using C. botulinum type B live spore antiserum. The whole cell proteins were separated by two dimensional gel electrophoresis and transferred to polyvinylidene difluoride membranes. Further, the Western blotting was performed with mouse pups immune serum against C. botulinum type B live spores. Eight predominant cross immunoreactive proteins were identified by mass spectrometry. These immunogenic proteins might be used to develop novel subunit vaccine candidates against the intestinal botulism.
authors.affiliation: Biotechnology Division, Defence Research & Development Establishment, Gwalior- 474 002, Madhyapradesh. India.
authors.forename: Arti
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Biotechnology Division, Defence Research & Development Establishment, Gwalior- 474 002, Madhyapradesh. India.
authors.forename: Sarita
authors.initials: S
authors.lastname: Rani
authors.affiliation: Biotechnology Division, Defence Research & Development Establishment, Gwalior- 474 002, Madhyapradesh. India.
authors.forename: Syed Imteyaz
authors.initials: SI
authors.lastname: Alam
authors.affiliation: Biotechnology Division, Defence Research & Development Establishmnet, Gwalior -474 002, M.P. India.
authors.forename: Sarkaraisamy
authors.initials: S
authors.lastname: Ponmariappan
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology / Q000302:isolation & purification*; D001906:Botulism / Q000097:blood / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D003014:Clostridium botulinum / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D057166:High-Throughput Screening Assays; D007106:Immune Sera / Q000737:chemistry; D007422:Intestines; D051379:Mice; D040901:Proteomics / Q000379:methods; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology*; D053719:Tandem Mass Spectrometry
chemical_list: D001428:Bacterial Vaccines; D001798:Blood Proteins; D007106:Immune Sera
keywords: Immunoproteomics; botulinum neurotoxins; botulism; immunogenic
  protein; mass-spectrometry; whole cell protein