title: Mitochondria-targeted metformins: anti-tumour and redox signalling mechanisms.
abstract: Reports suggest that metformin exerts anti-cancer effects in diabetic individuals with pancreatic cancer. Thus, metformin is currently being repurposed as a potential drug in cancer treatment. Studies indicate that potent metformin analogues are required in cancer treatment because of the low bioavailability of metformin in humans at conventional antidiabetic doses. We proposed that improved mitochondrial targeting of metformin by attaching a positively charged lipophilic triphenylphosphonium group will result in a new class of mitochondria-targeted metformin analogues with significantly enhanced anti-tumour potential. Using this approach, we synthesized various mitochondria-targeted metformin analogues with different alkyl chain lengths. Results indicate that the antiproliferative effects increased with increasing alkyl chain lengths (100-fold to 1000-fold). The lead compound, mito-metformin10, potently inhibited mitochondrial respiration through inhibition of complex I, stimulation of superoxide and hydrogen peroxide formation and activation of AMPK. When used in combination with ionizing radiation, mito-metformin10acted as a radiosensitizer of pancreatic cancer cells. Because of the 1000-fold-higher potency of mitochondria-targeted metformin10, therapeutically effective plasma concentrations likely can be achieved in cancer patients.
authors.affiliation: Department of Biophysics and Free Radical Research , Medical College of Wisconsin , Milwaukee, WI , USA.
authors.forename: Balaraman
authors.identifier: 0000-0002-9180-8296
authors.initials: B
authors.lastname: Kalyanaraman
authors.affiliation: Department of Biophysics and Free Radical Research , Medical College of Wisconsin , Milwaukee, WI , USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Aix Marseille Univ, CNRS, ICR, UMR 7273 , 13013 Marseille , France.
authors.forename: Micael
authors.initials: M
authors.lastname: Hardy
authors.affiliation: Aix Marseille Univ, CNRS, ICR, UMR 7273 , 13013 Marseille , France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Ouari
authors.affiliation: Institute of Applied Radiation Chemistry , Lodz University of Technology , Zeromskiego 116, 90-924 Lodz , Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Sikora
authors.affiliation: Department of Biophysics and Free Radical Research , Medical College of Wisconsin , Milwaukee, WI , USA.
authors.forename: Jacek
authors.initials: J
authors.lastname: Zielonka
authors.affiliation: Department of Microbiology and Molecular Genetics and Cancer Center , Medical College of Wisconsin , Milwaukee, WI , USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Dwinell
keywords: AMPK; anti-cancer agent; metformin; mitochondria; reactive oxygen species; redox signalling