title: In vitro safety and efficacy evaluations of a complex botanical mixture of Eugenia dysenterica DC. (Myrtaceae): Prospects for developing a new dermocosmetic product.
abstract: In the context of developing a new natural product-based cosmetic, the in vitro efficacy and safety evaluations of a complex botanical mixture based on Eugenia dysenterica leaf hydroalcoholic extract (EDE) (2.5-1000μg/mL) were carried out. Chromatographic analysis demonstrated the presence of the tannin (ellagic acid) and flavonoids (quercetin and gallic acid) which characterize the EDE as a polyphenol-rich mixture. Using HFF-1 fibroblasts, it was shown that EDE promoted cell regeneration after UVA exposure. It also led to the inhibition of the collagenase, elastase and tyrosinase enzymes, which are involved in skin-related disorders. In terms of toxicological evaluation, the EDE was classified as non-phototoxic through the 3T3 Neutral Red Uptake Phototoxicity Test (OECD N° 432, 2004) and non-eye irritant by Bovine Corneal Opacity and Permeability (OECD N° 437, 2013) assay, in conjunction with corneal histomorphometric analysis. Furthermore, the EDE has no skin sensitization potential as demonstrated by a two-out-of-three prediction model [protein-binding/haptenization (OECD N° 442C, 2015), keratinocyte and dendritic cell activations]. In addition, it was shown that the EDE seems to be non-genotoxic through the cytokinesis-block micronucleus assay (OECD N° 487, 2014) using HepG2 cells. When considered together, these findings support the use of EDE botanical mixture in cosmetic/pharmaceutical products.
authors.affiliation: Laboratory of Celullar Toxicology and Pharmacology - FarmaTec, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Larissa Cleres
authors.initials: LC
authors.lastname: Moreira
authors.affiliation: Laboratory of Celullar Toxicology and Pharmacology - FarmaTec, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Renato Ivan
authors.initials: RI
authors.lastname: de Ávila
authors.affiliation: Laboratory of Pharmaceutical Technology - FarmaTec, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Danillo Fabrini Maciel Costa
authors.initials: DFMC
authors.lastname: Veloso
authors.affiliation: Laboratory of Biological Activity, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Tatiana Nascimento
authors.initials: TN
authors.lastname: Pedrosa
authors.affiliation: Laboratory of Biological Activity, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, AM, Brazil.
authors.forename: Emerson Silva
authors.initials: ES
authors.lastname: Lima
authors.affiliation: Laboratory of Natural Products Research - LPPN, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Renê Oliveira
authors.initials: RO
authors.lastname: do Couto
authors.affiliation: Laboratory of Pharmaceutical Technology - FarmaTec, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Eliana Martins
authors.initials: EM
authors.lastname: Lima
authors.affiliation: Laboratory of Oral Pathology, Dental School, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Aline Carvalho
authors.initials: AC
authors.lastname: Batista
authors.affiliation: Laboratory of Natural Products Research - LPPN, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: José Realino
authors.initials: JR
authors.lastname: de Paula
authors.affiliation: Laboratory of Celullar Toxicology and Pharmacology - FarmaTec, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil. Electronic address: marizeufg@gmail.com.
authors.forename: Marize Campos
authors.initials: MC
authors.lastname: Valadares
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D045424:Complex Mixtures; D003257:Consumer Product Safety; D003315:Cornea / Q000187:drug effects; D003358:Cosmetics / Q000737:chemistry* / Q000633:toxicity*; D003713:Dendritic Cells / Q000187:drug effects; D017484:Dermatitis, Phototoxic; D003879:Dermatologic Agents / Q000737:chemistry* / Q000633:toxicity*; D000068856:Eugenia / Q000737:chemistry* / Q000633:toxicity*; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism; D007509:Irritants / Q000633:toxicity; D015603:Keratinocytes / Q000187:drug effects; D051379:Mice; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D045424:Complex Mixtures; D003358:Cosmetics; D003879:Dermatologic Agents; D020382:Interleukin-18; D007509:Irritants; D010936:Plant Extracts
keywords: Alternative methods; Anti-aging; Complex botanical mixture; Cosmetic; Risk evaluation; Skin-whitening