title: Alternative Formation of Red-Shifted Channelrhodopsins: Noncovalent Incorporation with Retinal-Based Enamine-Type Schiff Bases and Mutated Channelopsin.
abstract: Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with all-trans-retinal.
authors.affiliation: Department of Organic Chemistry for Life Science, Kobe Pharmaceutical University.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okitsu
authors.forename: Take
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Toru
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Hiromu
authors.initials: H
authors.lastname: Yawo
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Imamoto
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shichida
authors.forename: Akimori
authors.initials: A
authors.lastname: Wada
mesh_terms: D015394:Molecular Structure; D012172:Retinaldehyde / Q000737:chemistry*; D012243:Rhodopsin / Q000138:chemical synthesis* / Q000737:chemistry; D012545:Schiff Bases / Q000737:chemistry; D014212:Tretinoin / Q000737:chemistry*
chemical_list: D012545:Schiff Bases; D014212:Tretinoin; D012243:Rhodopsin; D012172:Retinaldehyde