title: Generation and characterization of two iPSC lines from human epicardium-derived cells.
abstract: Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.
authors.affiliation: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Paulitschek
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors.forename: Peggy
authors.initials: P
authors.lastname: Schulze-Matz
authors.affiliation: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Hesse
authors.affiliation: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Schmidt
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors.forename: Wasco
authors.initials: W
authors.lastname: Wruck
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
authors.affiliation: Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany. Electronic address: schrader@uni-duesseldorf.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schrader
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D016172:DNA Fingerprinting; D058732:Embryoid Bodies / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008856:Microscopy, Fluorescence; D010496:Pericardium / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors