title: Multiplex digital colour-coded barcode technology on RNA extracted from routine cytological samples of patients with non-small cell lung cancer: pilot study.
abstract: In the advanced stages of non-small cell lung cancer (NSCLC), molecular testing is often performed on archival cytological smears. The nCounter system (NanoString Technologies) is a new promising multiplex digital colour-coded barcode technology. However, its feasibility to evaluate the RNA expression of clinical relevant biomarkers on routine cytological smears is still uncertain. To this end, RNA was extracted from 12 NSCLC routine stained cytological smears, and nCounter analysis performed by using a 48-gene panel. Overall, 11/12 (92%) of the smears were adequate for the secondary analysis, fulfilling the quality check parameter analysis of nSolver software. This pilot study shows that RNA nCounter analysis is feasible on routine cytological smears preparing the field for the implementation of this technology in the routine setting.
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Sgariglia
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Pisapia
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Mariantonia
authors.initials: M
authors.lastname: Nacchio
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: De Luca
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pepe
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Russo
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Claudio
authors.identifier: http://orcid.org/0000-0002-7479-6457
authors.initials: C
authors.lastname: Bellevicine
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
authors.affiliation: Department of Public Health, University of Naples Federico II, Naples, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Malapelle
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D005240:Feasibility Studies; D020869:Gene Expression Profiling / Q000379:methods*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D025202:Molecular Diagnostic Techniques; D009367:Neoplasm Staging; D010641:Phenotype; D011237:Predictive Value of Tests; D012334:RNA, Neoplasm / Q000235:genetics*; D015203:Reproducibility of Results; D059467:Transcriptome; D057188:Workflow
chemical_list: D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm
keywords: CYTOLOGY; LUNG CANCER; MOLECULAR PATHOLOGY