title: [Evaluation of renal tissue ischemia-reperfusion injury with ultrasound radiation force and targeted microbubbles].
abstract: OBJECTIVE
  To study the sensitivity and feasibility of detecting microvascular inflammation in renal ischemia- reperfusion injury using microbubbles (MB) targeted to the intercellular adhesion molecule ICAM-1 and ultrasound radiation force (USRF).
  
  
  METHODS
  Mouse models of kidney ischemia-reperfusion were randomized into 5 groups with reperfusion time of 1, 3, 6, 12, and 24 h (IRlh, IR3h, IR6h, IR12h, and IR24h group, respectively). Each group was subdivided into targeted MB group (MBICAMgroup) and targeted MB +USRF group (MBICAM+USRFgroup). Kidney enhancement and the video intensity (VI) of the kidneys were compared among the groups.
  
  
  RESULTS
  In normal mice in either MBICAMor MBICAM+USRFgroup, no obvious enhancement of the kidney or significant increase in VI of the kidneys was observed (P=0.923). The kidneys were enhanced in all the mice with renal ischemia-reperfusion injury, and with the passage of time, the enhancement increased progressively. VI in the kidneys of mice with renal ischemia-reperfusion injury in MBICAM+USRFgroup increased more significantly compared with the MBICAMgroup. Significant difference in the VI was noted among the groups with different perfusion time but not between IR12h and IR24h groups.
  
  
  CONCLUSIONS
  Microbubbles targeted to ICAM-1 combined with USRF can effectively evaluate renal ischemia-reperfusion injury in mice and can be used for early evaluation of microvascular inflammation and other endothelial responses.
authors.affiliation: Department of Diagnostic Ultrasound, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: 155045292@qq.com.
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Wang
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Luo
authors.forename: Feng-Lin
authors.initials: FL
authors.lastname: Wu
mesh_terms: D000818:Animals; D018799:Intercellular Adhesion Molecule-1 / Q000737:chemistry*; D007668:Kidney; D007674:Kidney Diseases / Q000000981:diagnostic imaging*; D051379:Mice; D045423:Microbubbles; D015427:Reperfusion Injury / Q000000981:diagnostic imaging*; D000069453:Ultrasonic Waves
chemical_list: D018799:Intercellular Adhesion Molecule-1