title: Protein interaction networks at the host-microbe interface inDiaphorina citri, the insect vector of the citrus greening pathogen.
abstract: The Asian citrus psyllid (Diaphorina citri)is the insect vector responsible for the worldwide spread of 'CandidatusLiberibacter asiaticus' (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such thatD. citritransmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in theD. citriimmune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host-microbe relationships. Using protein interaction reporter technology, aD. citrihaemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with aD. citrimyosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact withD. citriproteins. These co-evolved protein interaction networks at the host-microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.
authors.affiliation: Robert W. Holley Center for Agriculture and Health, Emerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, NY, USA; Boyce Thompson Institute for Plant Research, Ithaca, NY, USA.
authors.forename: J S
authors.identifier: 0000-0003-1439-500X
authors.initials: JS
authors.lastname: Ramsey
authors.affiliation: Department of Genome Sciences , University of Washington , Seattle, WA , USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Chavez
authors.affiliation: Department of Genome Sciences , University of Washington , Seattle, WA , USA.
authors.forename: R
authors.initials: R
authors.lastname: Johnson
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY, USA; Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Hosseinzadeh
authors.affiliation: Boyce Thompson Institute for Plant Research , Ithaca, NY , USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Mahoney
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, NY, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Mohr
authors.affiliation: Boyce Thompson Institute for Plant Research , Ithaca, NY , USA.
authors.forename: F
authors.initials: F
authors.lastname: Robison
authors.affiliation: Department of Genome Sciences , University of Washington , Seattle, WA , USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhong
authors.affiliation: US Horticultural Research Laboratory, Subtropical Insects and Horticulture Research Unit , USDA Agricultural Research Service , Ft. Pierce, FL , USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Hall
authors.affiliation: Department of Genome Sciences , University of Washington , Seattle, WA , USA.
authors.forename: M
authors.initials: M
authors.lastname: MacCoss
authors.affiliation: Department of Genome Sciences , University of Washington , Seattle, WA , USA.
authors.forename: J
authors.initials: J
authors.lastname: Bruce
authors.affiliation: Robert W. Holley Center for Agriculture and Health, Emerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, NY, USA; Boyce Thompson Institute for Plant Research, Ithaca, NY, USA; Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
authors.forename: M
authors.initials: M
authors.lastname: Cilia
keywords: Asian citrus psyllid; citrus greening disease; endosymbiont; insect vector; proteomics; ‘Candidatus Liberibacter asiaticus’