title: Establishment of novel therapy to reduce progression of myopia in rats with experimental myopia by fibroblast transplantation on sclera.
abstract: Myopia is one of the most common visual disorders, and is characterized by a progressive axial elongation of the eye. Several methods have been tried to reduce the progression of axial elongation and myopia, but there are still no well-accepted procedures. We hypothesized that transplantation of fibroblasts on the sclera would lead to the synthesis of collagen fibrils on the sclera and reinforce it, and reduce the degree of axial elongation of eyes with form deprivation myopia. To examine this, we developed a form deprivation myopia model in albino Wistar rats and examined the effects of human fibroblasts (hFbs) transplantation on the sclera in the progression of myopia and axial elongation. We found that the form deprivation by eyelid suture induced a myopic shift and axial elongation associated with a thinner sclera and smaller-diameter collagen fibrils in Wistar rats. We also found that the transplanted hFbs synthesized type 1 collagen fibrils on the rat sclera, and these eyes with form deprivation had significantly reduced ocular elongation and myopic shift than the eyes without hFbs transplantation. Some of the synthesized collagen fibrils migrated into the sclera and had a bundle-like appearance and a stripe-like pattern, indicating they had mature characteristics. These findings suggest that the rat sclera was reinforced by the newly synthesized collagen fibrils and the axial elongation was reduced. These results can provide important information for the development of a therapy targeting myopia in humans. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Kosei
authors.initials: K
authors.lastname: Shinohara
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Hongding
authors.initials: H
authors.lastname: Liu
authors.affiliation: Instrumental Analysis Research Center, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Tomoka
authors.initials: T
authors.lastname: Ishida
authors.affiliation: Cellular Physiological Chemistry, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Nakahama
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Nagaoka
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Muka
authors.initials: M
authors.lastname: Moriyama
authors.affiliation: Cellular Physiological Chemistry, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Morita
authors.affiliation: Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Bunkyoku, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ohno-Matsui
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D005347:Fibroblasts / Q000637:transplantation*; D006801:Humans; D008297:Male; D009216:Myopia / Q000473:pathology* / Q000628:therapy*; D017208:Rats, Wistar; D012029:Refraction, Ocular; D012030:Refractive Errors; D012590:Sclera / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D003094:Collagen
keywords: collagen 1; fibroblast; form deprivation myopia; myopia; rat; sclera; transplantation