title: Spectrophotometric analysis of crown discoloration induced by two different sealers: AnIn vitrostudy.
abstract: BACKGROUND
  Endodontic materials have been assessed with regards to their potential for dental staining.
  
  
  OBJECTIVE
  To evaluate the coronal discoloration effect of Apexit Plus and Resino Seal in mandibular premolars using spectrophotometer.
  
  
  METHODS
  The study design was original research.
  
  
  METHODS
  Forty human single-rooted mandibular first premolar teeth were selected for the study. They were divided into four groups of ten specimens each. Group 1 - Apexit Plus sealer, Group 2 - Resino Seal sealer, Group 3 - positive control (Blood), and Group 4 - negative control (distilled water). Teeth were resected 3 mm below at cementoenamel junction. In all samples, access cavities were prepared, instrumented, and irrigated. Sealers were mixed and placed into the pulp chamber through cervical access and control groups were filled with blood and distilled water. Coronal orifices were sealed using self-cure glass ionomer cement. Samples were analyzed using spectrophotometer to determine the discoloration in each group at 10, 17, and 24 days interval.
  
  
  METHODS
  Statistical analysis used Wilcoxon signed-rank test.
  
  
  RESULTS
  All sealers used in the present study caused a progressive coronal discoloration over a time period of 17-24 days. There was statistically significant difference between resino seal sealer and apexit plus sealer , with resino seal showing greater coronal discoloration.
  
  
  CONCLUSIONS
  Resino seal sealer has greater potential to cause discoloration of crown as compared to apexit plus sealer over a period of time.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College, Nagpur, Maharashtra, India.
authors.forename: Rashmi Keshav
authors.initials: RK
authors.lastname: Chahande
authors.affiliation: Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College, Nagpur, Maharashtra, India.
authors.forename: Sanjay S
authors.initials: SS
authors.lastname: Patil
authors.affiliation: Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College, Nagpur, Maharashtra, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Gade
authors.affiliation: Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College, Nagpur, Maharashtra, India.
authors.forename: Rishikesh
authors.initials: R
authors.lastname: Meshram
authors.affiliation: Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College, Nagpur, Maharashtra, India.
authors.forename: Deepika J
authors.initials: DJ
authors.lastname: Chandhok
authors.affiliation: Department of Conservative Dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College, Nagpur, Maharashtra, India.
authors.forename: Deepa A
authors.initials: DA
authors.lastname: Thakur
mesh_terms: D001641:Bicuspid / Q000187:drug effects; D002126:Calcium Hydroxide / Q000009:adverse effects*; D006801:Humans; D010895:Pit and Fissure Sealants / Q000009:adverse effects*; D012117:Resins, Synthetic / Q000009:adverse effects*; D013053:Spectrophotometry; D019228:Tooth Crown / Q000187:drug effects*; D014075:Tooth Discoloration / Q000139:chemically induced* / Q000175:diagnosis*
chemical_list: C074418:Apexit; D010895:Pit and Fissure Sealants; D012117:Resins, Synthetic; D002126:Calcium Hydroxide