title: Effects of dietary resveratrol supplementation on hepatic and serum pro-/anti-inflammatory activity in juvenile GIFT tilapia, Oreochromis niloticus.
abstract: Dietary resveratrol (RES) supplementation may have some pharmacological effects including anti-inflammation. Previous studies have shown that Kupffer cell activation and apoptosis induction increases the transcription of pro- and anti-inflammatory cytokines. The main purpose of this study was to investigate the pro- and anti-inflammatory activities of 0.1 or 0.3 g/kg RES as a dietary supplement in juvenile freshwater tilapia (Oreochromis niloticus). The results showed that hepatic and serum immunoglobulin M (IgM) significantly decreased and increased while anti- and pro-inflammatory cytokines significantly increased and decreased, respectively, in the RES-treated groups. The expression of serum and hepatic IgM and anti-inflammatory cytokines [interleukin (IL)-10] and its inverse inhibitor interferon (IFN)-γ significantly increased while pro-inflammatory cytokine transcription significantly decreased. Hematoxylin-eosin staining and scanning electron microscopy revealed intestinal deformation, irregular goblet cells, and apoptotic cells in the 0.3 g/kg RES groups. RES (0.3 g/kg) also induced necrosis, apoptosis, reduction in Kupffer cell number, compressed sinusoids, and deformation of epidermal cells in the liver of the treated groups. In conclusion, the results of the present study show that high doses of RES were absorbed in the gut and then damaged the liver and intestinal tissue.
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China; Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China; Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Song
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China.
authors.forename: Shunlong
authors.initials: S
authors.lastname: Meng
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Fan
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China; Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Bing
authors.affiliation: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Wuxi, Jiangsu 214081, China; Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China. Electronic address: chenjz@ffrc.cn.
authors.forename: Jiazhang
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000633:toxicity*; D023681:Cichlids / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D019587:Dietary Supplements / Q000009:adverse effects; D007075:Immunoglobulin M / Q000032:analysis; D008099:Liver / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000633:toxicity*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007075:Immunoglobulin M; D013267:Stilbenes; D000077185:Resveratrol
keywords: Apoptosis; GIFT tilapia; Gene expression; Inflammatory cytokine; Resveratrol