title: An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection ofEscherichia coliO157: H7 andVibrio choleraeO1.
abstract: The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection ofE. coliO157:H7 andVibrio choleraeO1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy. The successful approach of the simultaneous detection and quantification of two bacteria by an electrochemical biosensor demonstrated here could be readily expanded for the estimation of a variety of other pathogenic bacteria, proteins, and nucleotides. Because of their high sensitivity, electrochemical biosensors may represent a new avenue for early diagnosis of diseases.
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Dermatology, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Fang
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China;; Department of Clinical Biochemistry, Laboratory Sciences, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liang
authors.affiliation: Department of Clinical Laboratory Science, College of Medical Laboratory, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Junsong
authors.initials: J
authors.lastname: Zheng
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D058726:Nanoconjugates; D041241:Vibrio cholerae O1 / Q000302:isolation & purification*
chemical_list: D058726:Nanoconjugates
keywords: Electrochemical biosensor; Escherichia coli O157:H7; Multifunctional nanoconjugates; Simultaneous detection; Vibrio cholerae O1.