title: Uptake and selective partitioning of dietary lipids to ovarian and muscle tissue of maturing female coho salmon, Oncorhynchus kisutch, during secondary oocyte growth.
abstract: Female coho salmon, Oncorhynchus kisutch, were fed one of two experimental feeds containing lipids with markedly different stable13C isotope signatures during the late cortical alveolus, lipid droplet, and vitellogenesis stages of secondary oocyte growth. Ovarian and muscle lipids fatty acid concentrations were significantly affected by treatment during all three stages of development. Stable13C isotope analyses confirmed that dietary lipids were incorporated into both ovarian and muscle lipids during all three stages and revealed that ovarian lipids were more affected than muscle lipids during vitellogenesis. Arachidonic acid (ARA) was incorporated into ovarian lipids at the highest rate of all fatty acids examined with the greatest uptake observed during the cortical alveolus and lipid droplet stages of development. Docosahexaenoic acid (DHA) was incorporated into ovarian lipids at the next highest rate with the greatest uptake observed during the lipid droplet stage of development. The presence of an ovary specific, fatty acid transfer mechanism is proposed. Results from this study demonstrate the ability to greatly alter the fatty acid composition of ovarian lipids through a dietary change during secondary oocyte growth and may be of great interest to producers of farmed salmon and salmon broodstock programs.
authors.affiliation: Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, 2725 Montlake Blvd. E., Seattle, WA 98112, USA. Electronic address: Ronald.B.Johnson@noaa.gov.
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Johnson
authors.affiliation: Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, 2725 Montlake Blvd. E., Seattle, WA 98112, USA.
authors.forename: Eric L
authors.initials: EL
authors.lastname: Kroeger
authors.affiliation: Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, 2725 Montlake Blvd. E., Seattle, WA 98112, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: Reichert
authors.affiliation: Aquaculture Research Institute, University of Idaho, c/o 2725 Montlake Blvd E., Seattle, WA 98112, USA.
authors.forename: Cameron S
authors.initials: CS
authors.lastname: Carter
authors.affiliation: Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, 2725 Montlake Blvd. E., Seattle, WA 98112, USA.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Rust
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000378:metabolism*; D005260:Female; D008055:Lipids / Q000737:chemistry*; D009132:Muscles / Q000378:metabolism*; D017684:Oncorhynchus kisutch / Q000254:growth & development* / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D009866:Oogenesis / Q000502:physiology; D010053:Ovary / Q000166:cytology* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D008055:Lipids
keywords: Broodstock nutrition; Fatty acids; Lipid transport; Secondary oogenesis; Stable isotopes