title: Elite Haplotypes of a Protein Kinase GeneTaSnRK2.3Associated with Important Agronomic Traits in Common Wheat.
abstract: Plant-specific protein kinase SnRK2s play crucial roles in response to various environmental stimuli.TaSnRK2.3, a SnRK2 member, was involved in the response to multiple abiotic stresses in wheat. To facilitate the use ofTaSnRK2.3in wheat breeding, the three genomic sequences ofTaSnRK2.3, originating from the A, B, and D genomes of hexaploid wheat, were obtained. Sequence polymorphism assays showing 4 and 10 variations were detected atTaSnRK2.3-1Aand atTaSnRK2.3-1B, respectively, yet no variation was identified atTaSnRK2.3-1D. Three haplotypes for A genome, and two main haplotypes for B genome ofTaSnRK2.3were identified in 32 genotypes. Functional markers (2.3AM1, 2.3AM2, 2.3BM1, 2.3BM2) were successfully developed to distinguish different haplotypes. Association analysis was performed with the general linear model in TASSEL 2.1. The results showed that bothTaSnRK2.3-1AandTaSnRK2.3-1Bwere significantly associated with plant height (PH), length of peduncle and penultimate node, as well as 1,000-grain weight (TGW) under different environments. Additionally,TaSnRK2.3-1Bwas significantly associated with stem water-soluble carbohydrates at flowering and mid-grain filling stages.Hap-1A-1 had higher TGW and lower PH;Hap-1B-1 had higher TGW and stem water-soluble carbohydrates, as well as lower PH, thus the two haplotypes were considered as elite haplotypes. Geographic distribution and allelic frequencies indicated that the two preferred haplotypesHap-1A-1 andHap-1B-1 were positively selected in the process of Chinese wheat breeding. These results could be valuable for genetic improvement and germplasm enhancement using molecular marker assisted selection in wheat breeding.
authors.affiliation: College of Agronomy, Northeast Agricultural University Harbin, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Miao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural SciencesBeijing, China; College of Life Science and Technology, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Mao
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Zicheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Li
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chang
authors.affiliation: International Maize and Wheat Improvement Center Texcoco, Mexico.
authors.forename: Matthew
authors.initials: M
authors.lastname: Reynolds
authors.affiliation: College of Agronomy, Northeast Agricultural University Harbin, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Ruilian
authors.initials: R
authors.lastname: Jing
keywords: TaSnRK2.3; association analysis; functional marker; haplotype; stem water-soluble carbohydrates