title: A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A.
abstract: The primary human dermal fibroblasts (PHDFs) from breast cancer patient were obtained to generate the human induced pluripotent stem cell line GPCCi001-A via lentiviral transfection. Thus, a modified EF1a-hSTEMCCA-loxP with tetO operator which regulates transgene expression was used. This method takes advantage of epigenetic regulation of transcription and allows for stable silencing of the reprogramming factors in obtained hiPS cells. To increase the potential utility of hiPSCs for clinical applications, they were adapted to feeder- and xeno-free conditions. The pluripotency of GPCCi001-A cell line and ability to differentiate into three germ layers was confirmed.
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Poznan, Poland; Postgraduate School of Molecular Medicine, Warsaw University of Medical Sciences, Warsaw, Poland.
authors.forename: Michał Stefan
authors.initials: MS
authors.lastname: Lach
authors.affiliation: Department of Oncologic Pathology and Prophylactics, Poznan University of Medical Sciences, Poznan, Poland; Department of Oncologic Pathology, Greater Poland Cancer Center, Poznan, Poland.
authors.forename: Joanna P
authors.initials: JP
authors.lastname: Wroblewska
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Poznan, Poland; Postgraduate School of Molecular Medicine, Warsaw University of Medical Sciences, Warsaw, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Augustyniak
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Poznan, Poland; Department of Electroradiology, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: katarzyna.kulcenty@wco.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kulcenty
authors.affiliation: Radiobiology Laboratory, Greater Poland Cancer Centre, Poznan, Poland; Department of Electroradiology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Wiktoria Maria
authors.initials: WM
authors.lastname: Suchorska
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D058732:Embryoid Bodies / Q000378:metabolism / Q000473:pathology; D063185:Epigenetic Repression; D061252:Feeder Cells / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D008856:Microscopy, Fluorescence; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors