title: Diplosporous development in Boehmeria tricuspis: Insights from de novo transcriptome assembly and comprehensive expression profiling.
abstract: Boehmeria tricuspis includes sexually reproducing diploid and apomictic triploid individuals. Previously, we established that triploid B. tricuspis reproduces through obligate diplospory. To understand the molecular basis of apomictic development in B. tricuspis, we sequenced and compared transcriptomic profiles of the flowers of sexual and apomictic plants at four key developmental stages. A total of 283,341 unique transcripts were obtained from 1,463 million high-quality paired-end reads. In total, 18,899 unigenes were differentially expressed between the reproductive types at the four stages. By classifying the transcripts into gene ontology categories of differentially expressed genes, we showed that differential plant hormone signal transduction, cell cycle regulation, and transcription factor regulation are possibly involved in apomictic development and/or a polyploidization response in B. tricuspis. Furthermore, we suggest that specific gene families are possibly related to apomixis and might have important effects on diplosporous floral development. These results make a notable contribution to our understanding of the molecular basis of diplosporous development in B. tricuspis.
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Gonggu
authors.initials: G
authors.lastname: Zang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Mingbao
authors.initials: M
authors.lastname: Luan
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Zemao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Lining
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Su
mesh_terms: D060832:Apomixis; D032402:Boehmeria / Q000235:genetics* / Q000254:growth & development*; D016000:Cluster Analysis; D030541:Databases, Genetic; D004171:Diploidy; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D010937:Plant Growth Regulators / Q000378:metabolism; D011123:Polyploidy; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators