title: New doxorubicin nanocarriers based on cyclodextrins.
abstract: Polymeric nanoparticles and fibrin gels (FBGs) are attractive biomaterials for local delivery of a variety of biotherapeutic agents, from drugs to proteins. We combined these different drug delivery approaches by preparing nanoparticle-loaded FBGs characterized by their intrinsic features of drug delivery rate and antiproliferative/apoptotic activities. Inclusion complexes of doxorubicin (DOXO) with oligomeric β-cyclodextrins (oCyD) functionalized with different functional groups were studied. These nanocarriers were able to interact with FBGs as shown by a decreased release rate of DOXO. One of these complexes, oCyDNH2/DOXO, demonstrated good antiproliferative and apoptotic activity in vitro, reflecting a higher drug uptake by cells. As hypothesized, the nanocarrier/FBG complexes showed a lower drug release rate than similar FBGs loaded with the corresponding non-functionalized oCyD/DOXO. Taken together, our results provide experimental evidence that oCyDNH2/DOXO complexes may be useful components in enhanced FBGs and further build support for the great promise these complex molecules hold for clinical use in localized anticancer therapy of inoperable or surgically removable tumors of different histological origin.
authors.affiliation: IRCCS A.O.U. San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, U.O.C. Bioterapie, L.go R. Benzi 10, 16132, Genoa, Italy. maurizio.viale@hsanmartino.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Viale
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, 95125, Catania, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Giglio
authors.affiliation: IRCCS A.O.U. San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, U.O.C. Bioterapie, L.go R. Benzi 10, 16132, Genoa, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Monticone
authors.affiliation: IRCCS A.O.U. San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, U.O.C. Bioterapie, L.go R. Benzi 10, 16132, Genoa, Italy.
authors.forename: Irena
authors.initials: I
authors.lastname: Maric
authors.affiliation: Dipartimento di Farmacia - Scienze del Farmaco, Università degli Studi di Bari 'Aldo Moro', Via E. Orabona 4, 70126, Bari, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Lentini
authors.affiliation: IRCCS A.O.U. San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, U.O.S. Biopolimeri e Proteomica, L.go R. Benzi 10, 16132, Genoa, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: Rocco
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, 95125, Catania, Italy.
authors.forename: Graziella
authors.initials: G
authors.lastname: Vecchio
mesh_terms: D045744:Cell Line, Tumor; D003505:Cyclodextrins / Q000097:blood / Q000494:pharmacology*; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D005782:Gels / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D004337:Drug Carriers; D005782:Gels; D011108:Polymers; D004317:Doxorubicin
keywords: Cyclodextrin nanocarrier; Doxorubicin; Fibrin gels; Localized treatment; Tumor targeting