title: The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing.
abstract: The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression duringArabidopsis thalianareproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression acrossA. thalianaaccessions.
authors.affiliation: Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912.
authors.forename: Alexander R
authors.identifier: http://orcid.org/0000-0003-3034-1482
authors.initials: AR
authors.lastname: Leydon
authors.affiliation: Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912.
authors.forename: Caleb
authors.identifier: http://orcid.org/0000-0001-6100-6084
authors.initials: C
authors.lastname: Weinreb
authors.affiliation: Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912.
authors.forename: Elena
authors.identifier: http://orcid.org/0000-0001-8463-0533
authors.initials: E
authors.lastname: Venable
authors.affiliation: Department of Plant and Microbial Biology, University of Minnesota, St. Paul, Minnesota 55108-6106.
authors.forename: Anke
authors.identifier: http://orcid.org/0000-0001-5351-3940
authors.initials: A
authors.lastname: Reinders
authors.affiliation: Department of Plant and Microbial Biology, University of Minnesota, St. Paul, Minnesota 55108-6106.
authors.forename: John M
authors.identifier: http://orcid.org/0000-0001-8185-0434
authors.initials: JM
authors.lastname: Ward
authors.affiliation: Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912.
authors.forename: Mark A
authors.identifier: http://orcid.org/0000-0002-8508-2724
authors.initials: MA
authors.lastname: Johnson
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D029681:Arabidopsis Proteins; D018749:RNA, Plant