title: CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters.
abstract: Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology, and Research (A*STAR), Singapore.
authors.forename: Mingzi M
authors.initials: MM
authors.lastname: Zhang
authors.affiliation: Molecular Engineering Lab, Biomedical Science Institutes, A*STAR, Singapore.
authors.forename: Fong Tian
authors.initials: FT
authors.lastname: Wong
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Shangwen
authors.initials: S
authors.lastname: Luo
authors.affiliation: Organic Chemistry, Institute of Chemical and Engineering Sciences, A*STAR, Singapore.
authors.forename: Yee Hwee
authors.initials: YH
authors.lastname: Lim
authors.affiliation: Molecular Engineering Lab, Biomedical Science Institutes, A*STAR, Singapore.
authors.forename: Elena
authors.initials: E
authors.lastname: Heng
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology, and Research (A*STAR), Singapore.
authors.forename: Wan Lin
authors.initials: WL
authors.lastname: Yeo
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Ryan E
authors.initials: RE
authors.lastname: Cobb
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Behnam
authors.initials: B
authors.lastname: Enghiad
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology, and Research (A*STAR), Singapore.
authors.forename: Ee Lui
authors.initials: EL
authors.lastname: Ang
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology, and Research (A*STAR), Singapore.|Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao