title: Screening and Validation of Novel Biomarkers in Osteoarticular Pathologies by Comprehensive Combination of Protein Array Technologies.
abstract: Osteoarthritis (OA) is one of the most prevalent articular diseases. The identification of proteins closely associated with the diagnosis, progression, prognosis, and treatment response is dramatically required for this pathology. In this work, differential serum protein profiles have been identified in OA and rheumatoid arthritis (RA) by antibody arrays containing 151 antibodies against 121 antigens in a cohort of 36 samples. Then the identified differential serum protein profiles have been validated in a larger cohort of 282 samples. The overall immunoreactivity is higher in the pathological situations in comparison with the controls. Several proteins have been identified as biomarker candidates for OA and RA. Most of these biomarker candidates are proteins related to inflammatory response, lipid metabolism, or bone and extracellular matrix formation, degradation, or remodeling.
authors.forename: Álvaro
authors.identifier: 0000-0003-4511-408X
authors.initials: Á
authors.lastname: Sierra-Sánchez
authors.forename: Diego
authors.identifier: 0000-0002-4131-4458
authors.initials: D
authors.lastname: Garrido-Martín
authors.affiliation: Proteomics Group-PBR2-ProteoRed/ISCIII, Rheumatology Division, Instituto de Investigación Biomédica de A Coruña (INIBIC/CHUAC/Sergas/UDC) , 15001 A Coruña, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Lourido
authors.forename: María
authors.identifier: 0000-0001-8115-1086
authors.initials: M
authors.lastname: González-González
authors.forename: Paula
authors.identifier: 0000-0003-2150-6898
authors.initials: P
authors.lastname: Díez
authors.affiliation: Proteomics Group-PBR2-ProteoRed/ISCIII, Rheumatology Division, Instituto de Investigación Biomédica de A Coruña (INIBIC/CHUAC/Sergas/UDC) , 15001 A Coruña, Spain.
authors.forename: Cristina
authors.identifier: 0000-0001-7649-9803
authors.initials: C
authors.lastname: Ruiz-Romero
authors.affiliation: Affinity Proteomics, Science for Life Laboratory, School of Biotechnology, Royal Institute of Technology (KTH) , SE-17165 Stockholm, Sweden.
authors.forename: Ronald
authors.identifier: 0000-0003-1363-5796
authors.initials: R
authors.lastname: Sjöber
authors.forename: Conrad
authors.identifier: 0000-0001-6027-5805
authors.initials: C
authors.lastname: Droste
authors.forename: Javier
authors.identifier: 0000-0002-0984-9946
authors.initials: J
authors.lastname: De Las Rivas
authors.affiliation: Affinity Proteomics, Science for Life Laboratory, School of Biotechnology, Royal Institute of Technology (KTH) , SE-17165 Stockholm, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
authors.affiliation: Proteomics Group-PBR2-ProteoRed/ISCIII, Rheumatology Division, Instituto de Investigación Biomédica de A Coruña (INIBIC/CHUAC/Sergas/UDC) , 15001 A Coruña, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Blanco
authors.forename: Manuel
authors.identifier: 0000-0002-7305-3766
authors.initials: M
authors.lastname: Fuentes
mesh_terms: D000906:Antibodies; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D018450:Disease Progression; D006801:Humans; D010003:Osteoarthritis / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D011379:Prognosis; D040081:Protein Array Analysis / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D000906:Antibodies; D015415:Biomarkers
keywords: antibody arrays; biomarkers; contact printing; noncontact printing; osteoarthritis; rheumatoid arthritis; serum protein profiles