title: Differential Regulation of chTLR3 by Infectious Bursal Disease Viruses with Different Virulence In Vitro and In Vivo.
abstract: Toll-like receptor 3 (TLR3) is one of the TLRs whose ligand is double-stranded RNA (dsRNA). Infectious bursal disease virus (IBDV) is a dsRNA virus that could be recognized by TLR3. The purpose of this study was to determine the role of the virulence of IBDV on the expression of chicken TLR3 (chTLR3). For this purpose, the levels of chTLR3 expression and its downstream effectors, Interferon β (IFN-β) and Interleukin 8 (IL-8), were detected and analyzed after infection of IBDV field isolates with differential virulence in vitro (chicken embryo fibroblast and/or chicken peripheral blood mononuclear cells) and in vivo (commercial Three-Yellow chicken). The results showed that chTLR3 was activated by IBDV, resulting in the expression of antiviral IFN-β and chemokine IL-8. The expression of chTLR3, IFN-β, and IL-8 correlated well with the virulence of IBDV as the more virulent the IBDV strain that was used, the more pronounced was the expression of chTLR3, IFN-β, and IL-8. These results suggest that chTLR3 is involved in the pathogenesis of IBDV in commercial chickens and its downstream effectors (IFN-β and IL-8) might play an important role in this process.
authors.affiliation: 1 School of Marine Sciences and Biotechnology/Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities , Nanning, China .|2 Institute for Poultry Science and Health, Guangxi University , Nanning, China .|3 Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and Their Modification, Guangxi University for Nationalities , Nanning, China .
authors.forename: Xiumiao
authors.initials: X
authors.lastname: He
authors.affiliation: 1 School of Marine Sciences and Biotechnology/Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities , Nanning, China .
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 1 School of Marine Sciences and Biotechnology/Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi University for Nationalities , Nanning, China .
authors.forename: Synat
authors.initials: S
authors.lastname: Kang
authors.affiliation: 2 Institute for Poultry Science and Health, Guangxi University , Nanning, China .
authors.forename: Guo
authors.initials: G
authors.lastname: Chen
authors.affiliation: 2 Institute for Poultry Science and Health, Guangxi University , Nanning, China .
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D002645:Chickens / Q000821:virology*; D005786:Gene Expression Regulation / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000276:immunology / Q000472:pathogenicity*; D016899:Interferon-beta / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D012333:RNA, Messenger; D012367:RNA, Viral; D013997:Time Factors; D051196:Toll-Like Receptor 3 / Q000235:genetics*; D014774:Virulence / Q000276:immunology; D014779:Virus Replication
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger; D012367:RNA, Viral; D051196:Toll-Like Receptor 3; D016899:Interferon-beta
keywords: IFN-β; IL-8; chicken toll-like receptor 3; infectious bursal disease virus; three-yellow chicken