title: Effects of TGFβ1, PDGF-BB, and bFGF, on human corneal fibroblasts proliferation and differentiation during stromal repair.
abstract: In an effort to improve the regenerative nature of corneal repair, this study reports the use of an in vitro human corneal fibroblasts (HCFs) wound model after treatment with three of the main growth factors (GFs) involved in corneal healing: transforming growth factor beta 1 (TGFβ1), platelet-derived growth factor BB-isoform (PDGF-BB), and basic fibroblast growth factor (bFGF) in order to delve in cell proliferation and differentiation processes. HCFs were mechanically wounded. The individual effect of TGFβ1, PDGF-BB, and bFGF on cell proliferation and differentiation during the repair process was studied at different time points until wound closure. Wound dimensions and morphological changes were evaluated by microscopy. Cell proliferation and myofibroblast differentiation were analyzed by immunofluorescence cytochemistry. Changes in cell morphology were apparent at Day 4. PDGF-BB- and bFGF-treated cells had fibroblast-like morphology. TGFβ1 stimulated proliferation in the wound edge and surrounding area, induced myofibroblast differentiation and inhibited cellular migration. PDGF-BB induced rapid wound closure due to proliferation, high motility, and late myofibroblast differentiation. The time course of closure induced by bFGF was similar to that for PDGF-BB, but was mostly due to proliferation in the wound area, and inhibited myofibroblast differentiation. Each of the GFs induced increases in responses promoting stromal repair differently. This study provides insight regarding how to optimize the outcome of stromal repair following corneal injury.
authors.affiliation: Departamento de Biología Celular, Histología y Farmacología, Facultad de Medicina, Grupo de Investigación Reconocido: Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain. Electronic address: patricia.gallego.munoz@uva.es.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gallego-Muñoz
authors.affiliation: Departamento de Biología Celular, Histología y Farmacología, Facultad de Medicina, Grupo de Investigación Reconocido: Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain; Servicio de Oftalmología, Hospital Clínico Universitario de Valladolid, Valladolid, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Ibares-Frías
authors.affiliation: Departamento de Estadística, Facultad de Ciencias, Universidad de Valladolid, Valladolid, Spain.
authors.forename: María Cruz
authors.initials: MC
authors.lastname: Valsero-Blanco
authors.affiliation: Departamento de Biología Celular, Histología y Farmacología, Facultad de Medicina, Grupo de Investigación Reconocido: Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cantalapiedra-Rodriguez
authors.affiliation: Instituto Oftalmológico Fernández-Vega, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Merayo-Lloves
authors.affiliation: Departamento de Biología Celular, Histología y Farmacología, Facultad de Medicina, Grupo de Investigación Reconocido: Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martínez-García
mesh_terms: D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003315:Cornea / Q000166:cytology* / Q000187:drug effects; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D058628:Myofibroblasts / Q000187:drug effects* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2
keywords: Basic fibroblast growth factor (bFGF); Corneal stroma repair; Human corneal fibroblasts; Myofibroblasts, transforming growth factor beta 1 (TGFβ1); Platelet–derived growth factor BB-isoform (PDGF-BB)