title: An efficient rapid system for assaying HBx-mediated transactivation.
abstract: OBJECTIVE
  To develop a rapid and accurate assay system for screening inhibitors or enhancing agents targeting the transactivation capability of hepatitis B virus X protein (HBx) that activates cellular promoters in host cells to facilitate viral replication.
  
  
  RESULTS
  We constructed a new GFP-based reporter system which was different from a luciferase-based reporter system. Firstly, a FLAG-tagged HBx gene was inserted into an expression plasmid, resulting in plasmid pHBx. Next, HBx-FLAG was linked to EGFP by the internal ribosome entry site resulting in plasmid pHBxE. The transactivation effect of HBx-flag on cytomegalovirus (CMV) promoter was verified by EGFP expression using fluorescence quantitation and qPCR. Furthermore, the transactivation ability of the HBx gene was quantified by flow cytometry. Finally, this assay system was tested by known regulators of HBx including DDB1, ID1, and P53. As expected, the GFP reporter level in 293T cells changed with the increasing of HBx regulators. Furthermore, the system modeling the function of transactivation repressor in Hep3B, a HBV-integrated cell line.
  
  
  CONCLUSIONS
  Collectively, the GFP-based reporter system provides a rapid and accurate approach for analyzing transactivation ability of HBx.
authors.affiliation: Stem Cell P2 Laboratory, Shantou University Medical College, Shantou, 515041, People's Republic of China.|The Center for Reproductive Medicine, Shantou University Medical College, Shantou, 515041, People's Republic of China.|Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou, 515041, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Stem Cell P2 Laboratory, Shantou University Medical College, Shantou, 515041, People's Republic of China.|The Center for Reproductive Medicine, Shantou University Medical College, Shantou, 515041, People's Republic of China.|Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou, 515041, People's Republic of China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Shi
authors.affiliation: Stem Cell P2 Laboratory, Shantou University Medical College, Shantou, 515041, People's Republic of China.|The Center for Reproductive Medicine, Shantou University Medical College, Shantou, 515041, People's Republic of China.|Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou, 515041, People's Republic of China.
authors.forename: Shaozhe
authors.initials: S
authors.lastname: Yang
authors.affiliation: Stem Cell P2 Laboratory, Shantou University Medical College, Shantou, 515041, People's Republic of China. Pingnan_sun@yahoo.com.|The Center for Reproductive Medicine, Shantou University Medical College, Shantou, 515041, People's Republic of China. Pingnan_sun@yahoo.com.|Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou, 515041, People's Republic of China. Pingnan_sun@yahoo.com.
authors.forename: Pingnan
authors.initials: P
authors.lastname: Sun
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D013194:Staining and Labeling / Q000379:methods; D015534:Trans-Activators / Q000378:metabolism*; D015533:Transcriptional Activation; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C485184:enhanced green fluorescent protein; C057628:hepatitis B virus X protein; D049452:Green Fluorescent Proteins
keywords: Cytomegalovirus promoter; GFP; HBx gene; Hepatitus B virus X protein; Transactivation; Virus X protein