title: Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.
abstract: Functional complementation of cellular defects has been a valuable approach for localizing causative genes to specific chromosomes. The complementation strategy was followed by positional cloning and characterization of genes for their biological relevance. We herein describe strategies used for the construction of monochromosomal hybrids and their applications for cloning and characterization of genes related to cell growth, cell senescence and DNA repair. We have cloned RNaseT2, GluR6 (glutamate ionotropic receptor kainate type subunit 2-GRIK2) and protein tyrosine phosphatase, receptor type K (PTPRK) genes using these strategies.
authors.affiliation: Department of Basic Medical Sciences, Western University of Health Sciences, Pomona, CA, U.S.A. rathwal@temple.edu rkandpal@westernu.edu.
authors.forename: Raj P
authors.initials: RP
authors.lastname: Kandpal
authors.affiliation: Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, U.S.A.
authors.forename: Arbans K
authors.initials: AK
authors.lastname: Sandhu
authors.affiliation: Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, U.S.A.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, U.S.A.
authors.forename: Gursurinder P
authors.initials: GP
authors.lastname: Kaur
authors.affiliation: Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA, U.S.A. rathwal@temple.edu rkandpal@westernu.edu.
authors.forename: Raghbir S
authors.initials: RS
authors.lastname: Athwal
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002877:Chromosomes, Human / Q000235:genetics*; D018014:Gene Transfer Techniques; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000378:metabolism*; D008957:Models, Genetic; D018092:Kainic Acid Receptors / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics; D000097806:GluK2 Kainate Receptor
chemical_list: D018092:Kainic Acid Receptors; D012260:Ribonucleases; D025521:Tumor Suppressor Proteins; D000097806:GluK2 Kainate Receptor; C425232:RNASET2 protein, human
keywords: Chromosome transfer; GRIK-2; GluR6; cell senescence; functional cloning; glutamate ionotropic receptor kainate type subunit 2; monochromosomal hybrids; protein tyrosine phosphatase; receptor type K (PTPRK); review; senescence associated β-galactosidase