title: Polyhydroxybutyrate and polyhydroxydodecanoate produced by Burkholderia contaminans IPT553.
abstract: OBJECTIVE
  In this paper, we introduce a new Burkholderia contaminans capable of producing a newly characterized polymer.
  
  
  RESULTS
  CG-MS and magnetic nuclear resonance1H and13C were used to determine the constitution of polymers produced in glucose, glucose with casein, sucrose and sucrose with casein. Three pairs of primers were used to find the polyhydroxyalkanoates (PHA) synthase class and sequence. The synthesized polymers were composed by short-chain length PHA (scl-PHA), especially polyhydroxybutyrate (PHB), and medium chain length PHA (mcl-PHA), especially polyhydroxydodecanoate (PHDd), and their concentration, constitution and molecular weight depend on carbon source used. The bacterium showed only class I synthase which could not explain the mcl-PHA production.
  
  
  CONCLUSIONS
  Burkholderia contaminans has a class I PHA synthase and produces PHB combined to PHDd when cultivated in sucrose or glucose, and PHDd concentration is affected when casein is used.
  
  
  CONCLUSIONS
  PHA are natural polymers produced by a wide range of bacteria. The presence of PHDd monomers confers to the polymer elastomeric properties. Previously, PHDd was only obtained when bacteria were cultivated in related carbon source. In this work, B. contaminansIPT553 produced PHB with PHDd using simple and low-cost carbon sources that can make possible the cheaper production of a more flexible biopolymer with crystallinity and elasticity different from the more common PHAs.
authors.affiliation: Universidade de São Paulo, Instituto de Ciências Biomédicas IV, Programa de Pós-Graduação Interunidades em Biotecnologia IPT-USP-Butantan, São Paulo, São Paulo, Brazil.|Laboratório de Biotecnologia Industrial, Instituto de Pesquisas Tecnológicas do Estado de São Paulo, São Paulo, São Paulo, Brazil.|Departamento de Ciências Animais, Laboratório de Nanobiotecnologia, Universidade Federal Rural do Semi-Árido, Rio Grande do Norte, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Matias
authors.affiliation: Divisão de Ciências Fisiológicas e Químicas, Serviço de Química Orgânica, Instituto Butantan, São Paulo, São Paulo, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Brandt
authors.affiliation: Laboratório de Biotecnologia Industrial, Instituto de Pesquisas Tecnológicas do Estado de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: E S
authors.initials: ES
authors.lastname: da Silva
authors.affiliation: Laboratório de Biotecnologia Industrial, Instituto de Pesquisas Tecnológicas do Estado de São Paulo, São Paulo, São Paulo, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: de Andrade Rodrigues
keywords: 
  Burkholderia contaminans
  ; 
  PHA
  ; PHDd; biopolymer; polyhydroxyalkanoates