title: Enhanced Probiotic Potential ofLactobacillus reuteriWhen Delivered as a Biofilm on Dextranomer Microspheres That Contain Beneficial Cargo.
abstract: As with all orally consumed probiotics, the Gram-positive bacteriumLactobacillus reuteriencounters numerous challenges as it transits through the gastrointestinal tract of the host, including low pH, effectors of the host immune system, as well as competition with commensal and pathogenic bacteria, all of which can greatly reduce the availability of live bacteria for therapeutic purposes. Recently we showed thatL. reuteri, when adhered in the form of a biofilm to a semi-permeable biocompatible dextranomer microsphere, reduces the incidence of necrotizing enterocolitis by 50% in a well-defined animal model following delivery of a single prophylactic dose. Herein, using the same semi-permeable microspheres, we showed that providing compounds beneficial toL. reuterias diffusible cargo within the microsphere lumen resulted in further advantageous effects including glucosyltransferase-dependent bacterial adherence to the microsphere surface, resistance of bound bacteria against acidic conditions, enhanced adherence ofL. reuterito human intestinal epithelial cellsin vitro, and facilitated production of the antimicrobial compound reuterin and the anti-inflammatory molecule histamine. These data support continued development of this novel probiotic formulation as an adaptable and effective means for targeted delivery of cargo beneficial to the probiotic bacterium.
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Navarro
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Mashburn-Warren
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.
authors.forename: Lauren O
authors.initials: LO
authors.lastname: Bakaletz
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's HospitalColumbus, OH, USA; Wexner Medical Center, Institute for Behavioral Medicine Research, The Ohio State UniversityColumbus, OH, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Bailey
authors.affiliation: Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Goodman
keywords: Lactobacillus reuteri; dextranomer; glucosyltransferase; maltose; microsphere; reuterin