title: Characterization of chiral amino acids from different milk origins using ultra-performance liquid chromatography coupled to ion-mobility mass spectrometry.
abstract: Milk contains free amino acids (AAs) that play essential roles in maintaining the growth and health of infants, and D-AA isomers are increasingly being recognized as important signalling molecules. However, there are no studies of the different characteristics of chiral AA (C-AA) from different milk origins. Here, UPLC coupled to ion-mobility high-resolution MS (IM-HRMS) was employed to characterize 18 pairs of C-AAs in human, cow, yak, buffalo, goat, and camel milk. The results proved that milk origins can be differentiated based on the D- to L- AA ratio-based projection scores by principal component analysis. The present study gives a deeper understanding of the D- to L- AA ratio underlying the biological functions of different animal milks, and provide a new strategy for the study of AA metabolic pathways.
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: He
authors.initials: H
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: Songli
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: Yangdong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: Shengguo
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Laboratory of Quality&Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D000596:Amino Acids