title: Inhibition of Grass Carp Reovirus Infection by DNA Aptamers against S10 Protein.
abstract: Grass Carp reovirus (GCRV) is one of the most pathogenic agents among aquareovirus isolates and has the ability to cause a severe epidemic outbreak of hemorrhagic disease, thus resulting in both a high mortality rate during the culture of Grass Carp Ctenopharyngodon idella and an enormous economic loss. Aptamers have been demonstrated to have strong promising applications in antiviral drug development. In the present study, a complementary DNA fragment encoding the S10 gene of GCRV was cloned. The S10 protein was expressed and purified. Aptamers for S10 protein were selected by the method of selective evolution of ligands by exponential enrichment (SELEX), and their characteristics and antiviral actions were examined. All targeting-selected aptamers formed a similar structure, forming a 5-7 base loop at the terminus. The results show that the aptamers could inhibit the GCRV infection. The most significant inhibitory effect was obsereved when the aptamers were added to the cell culture for 1 h before the cells were infected by GCRV. Our data showed that these novel molecular agents could be considered suitable candidates for anti-GCRV therapy. Received August 23, 2016; accepted February 5, 2017.
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan 430070 , China.
authors.forename: Hongru
authors.initials: H
authors.lastname: Liang
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan 430070 , China.
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Fu
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan 430070 , China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Liu
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan 430070 , China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan 430070 , China.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Guo
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.affiliation: c Guangdong Haid Animal Husbandry and Veterinary Institute , Guangzhou 511400 , China.
authors.forename: JiaXu
authors.initials: J
authors.lastname: Liu
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Huang
authors.affiliation: a Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development , Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology , Guangdong Province , Guangzhou 510380 , China.|b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan 430070 , China.
authors.forename: Ningqiu
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000037:antagonists & inhibitors*; D002347:Carps; D005393:Fish Diseases / Q000517:prevention & control*; D012087:Reoviridae / Q000235:genetics*; D012088:Reoviridae Infections / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D052157:Aptamers, Nucleotide