title: Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytes.
abstract: In this study, microRNA (miRNA) profiles in postovulatory aging mouse oocytes were analyzed by microarray screening and RT-qPCR. Hierarchical cluster analysis on the microarray data and KEGG pathway enrichment analysis on the mRNAs targeted by differentially expressed (DE) miRNAs between two adjacent egg-ages suggest that while only a mild alteration in miRNA expression occurred from 13 to 18 h, a great change took place from 18 to 24 h post hCG injection. Theoretical exploration on functions of the predicted target genes suggest that KEGG pathways enriched by 13-18 h DE miRNAs are correlated with early events of oocyte aging while pathways most enriched by 18-24 h or 24-30 h DE miRNAs are correlated with the late symptoms of aged oocytes. Experimental verification on functions of the key proteins predicted by the KEGG analysis and injection of miR-98 mimics or inhibitors further confirmed that miRNAs played stimulatory/inhibitory roles in postovulatory oocyte aging. In conclusion, marked changes in miRNA expression are associated with significant alterations in function and morphology of postovulatory aging oocytes.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Tian-Yang
authors.initials: TY
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Hua-Yu
authors.initials: HY
authors.lastname: Lian
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Ming-Jiu
authors.initials: MJ
authors.lastname: Luo
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, P. R. China.
authors.forename: Jing-He
authors.initials: JH
authors.lastname: Tan
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D010060:Ovulation; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
chemical_list: D035683:MicroRNAs; C505409:Casp3 protein, mouse; D053148:Caspase 3; D002118:Calcium
keywords: aging; microRNA; molecular function; oocyte; signaling pathway