title: The choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system.
abstract: The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this study, we compared four variants of B. subtilis spores presenting a fragment of a FliD protein from Clostridium difficile in fusion with CotB, CotC, CotG or CotZ spore coat proteins. We show that these spores promote their own phagocytosis and activate both, the J774 macrophages and JAWSII dendritic cells of murine cell lines. Moreover, we used these spores for mucosal immunization of mice. We conclude that the observed effects vary with the type of displayed FliD-spore coat protein fusion and seem to be mostly independent of its abundance and localization in the spore coat structure.
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
authors.forename: Aurelia
authors.initials: A
authors.lastname: Piekarska
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Pełka
authors.affiliation: Tri-City Animal Laboratory, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Peszyńska-Sularz
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Negri
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Hinc
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Obuchowski
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Iwanicki
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology; D001412:Bacillus subtilis / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D016360:Clostridioides difficile / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology; D010587:Phagocytosis / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology; D013171:Spores, Bacterial / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014611:Vaccination
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; C439051:CotB protein, Bacillus subtilis; D011993:Recombinant Fusion Proteins; C091352:cotG protein, Bacillus subtilis; C076733:FlaD protein, Bacteria
keywords: APCs; Bacillus subtilis; Clostridium difficile; FliD; immune response; spore display