title: Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview.
abstract: The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.
authors.affiliation: 1 Spanish HIV HGM BioBank, Hospital General Universitario Gregorio Marañón , Instituto de Investigación Sanitaria Gregorio Marañón, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain .
authors.forename: Irene
authors.initials: I
authors.lastname: Consuegra
authors.affiliation: 2 Biobanco Vasco para la Investigación-OEHUN , Vizcaya, Spain .
authors.forename: Clara
authors.initials: C
authors.lastname: Rodríguez-Aierbe
authors.affiliation: 3 Instituto de Investigación Marqués de Valdecilla , Biobanco Valdecilla, Santander, Spain .
authors.forename: Inés
authors.initials: I
authors.lastname: Santiuste
authors.affiliation: 4 Biobanco Hospital Clínic-IDIBAPS , Institut d'Investigacions Biomèdiques August Pi i Sunyer, IDIBAPS, Barcelona, Spain .
authors.forename: Anna
authors.initials: A
authors.lastname: Bosch
authors.affiliation: 5 Hospital Virgen de la Arrixaca , BIOBANC-MUR, Murcia, Spain .
authors.forename: Rosario
authors.initials: R
authors.lastname: Martínez-Marín
authors.affiliation: 6 Clínica Universidad de Navarra , Biobanco de la Universidad de Navarra, Pamplona, Spain .
authors.forename: Ma Antonia
authors.initials: MA
authors.lastname: Fortuto
authors.affiliation: 7 Hospital Regional Universitario Carlos Haya , Málaga, Spain .
authors.forename: Tatiana
authors.initials: T
authors.lastname: Díaz
authors.affiliation: 8 Centro de Investigación Biomédica en Red de Enfermedades Raras , CIBERER BIOBANK, Valencia, Spain .
authors.forename: Salvador
authors.initials: S
authors.lastname: Martí
authors.affiliation: 1 Spanish HIV HGM BioBank, Hospital General Universitario Gregorio Marañón , Instituto de Investigación Sanitaria Gregorio Marañón, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain .
authors.forename: Ma Ángeles
authors.initials: MÁ
authors.lastname: Muñoz-Fernández
mesh_terms: D018070:Biological Specimen Banks; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D013030:Spain
keywords: PBMCs; SOP; biobanks; cryopreservation; functionality