title: Properties of Mitotic and Meiotic Recombination in the Tandemly-RepeatedCUP1Gene Cluster in the YeastSaccharomyces cerevisiae.
abstract: In the yeastSaccharomyces cerevisiae, the genes encoding the metallothionein protein Cup1 are located in a tandem array on chromosome VIII. Using a diploid strain that is heterozygous for an insertion of a selectable marker (URA3) within this tandem array, and heterozygous for markers flanking the array, we measured interhomolog recombination and intra/sister chromatid exchange in theCUP1locus. The rate of intra/sister chromatid recombination exceeded the rate of interhomolog recombination by >10-fold. Loss of the Rad51 and Rad52 proteins, required for most interhomolog recombination, led to a relatively small reduction of recombination in theCUP1array. Although interhomolog mitotic recombination in theCUP1locus is elevated relative to the average genomic region, we found that interhomolog meiotic recombination in the array is reduced compared to most regions. Lastly, we showed that high levels of copper (previously shown to elevateCUP1transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in theCUP1array; recombination events that delete theURA3insertion from theCUP1array occur at a rate of >10-3/division in unselected cells. This rate is almost three orders of magnitude higher than observed for mitotic recombination events involving single-copy genes. In summary, our study shows that some of the basic properties of recombination differ considerably between single-copy and tandemly-repeated genes.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710.|University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710.|University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Margaret
authors.initials: M
authors.lastname: Dominska
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710.|University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Petrova
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710.|University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Halle
authors.initials: H
authors.lastname: Bagshaw
authors.affiliation: Physical Sciences Directorate, United States Army Research Office, Research Triangle Park, North Carolina 27709.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kokoska
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710 tom.petes@duke.edu.|University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D059765:Homologous Recombination / Q000235:genetics*; D008540:Meiosis / Q000235:genetics; D008668:Metallothionein / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D051135:Rad51 Recombinase / Q000235:genetics; D051721:Rad52 DNA Repair and Recombination Protein / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D012854:Sister Chromatid Exchange; D020080:Tandem Repeat Sequences
chemical_list: C500769:CUP1-1 protein, S cerevisiae; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins; C554167:URA3 protein, S cerevisiae; D008668:Metallothionein; D051135:Rad51 Recombinase
keywords: meiotic recombination; mitotic recombination; repeated genes; sister chromatid crossovers