title: Gold nanoparticles modified porous silicon chip for SALDI-MS determination of glutathione in cells.
abstract: The gold nanoparticles (Au NPs) modified porous silicon chip based surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) was developed to capture and analyze glutathione (GSH) in cells. With silver-assisted chemical etching, Ag nanoparticles (Ag NPs) were generated and deposited on the silicon surface and the nanopores were etched on silicon substrate. Then Au NPs were in-situ synthesized on the ridges of silicon nanopores. This Ag-Au NPs modified porous silicon surface could specially capture and enrich thiol compounds through Au-S binding, and it could also function as matrix to assist ionization for SALDI-MS. The silicon chip was array patterned for high throughput SALDI-MS detection. GSH and cysteine could be distinguished without the interference from matrix signals. This approach was successfully applied to preconcentration and detection of GSH in Caco-2 cells. The GSH alterations in cells under drug stimulation were investigated. This invented silicon chip showed great potential for more efficient analysis of small thiol biomarkers in complex biological samples.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China; Graduate School of Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
authors.forename: Mingsha
authors.initials: M
authors.lastname: Jie
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China. Electronic address: lihaifang@mail.tsinghua.edu.cn.
authors.forename: Haifang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
authors.forename: Luyao
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China. Electronic address: jmlin@mail.tsinghua.edu.cn.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D018938:Caco-2 Cells; D005978:Glutathione / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D058608:Nanopores; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D006046:Gold; D005978:Glutathione; D012825:Silicon
keywords: Cells; Glutathione; Gold nanoparticles; Laser desorption/ionization mass spectrometry; Porous silicon