title: Development and Validation of Ion Chromatography-Tandem Mass Spectrometry-Based Method for the Multiresidue Determination of Polar Ionic Pesticides in Food.
abstract: An extraction method using acidified methanol based on the quick polar pesticide (QuPPe) method using suppressed ion chromatography coupled to mass spectrometry was developed and validated for the direct analysis of polar pesticides, without the need for derivatization or ion pairing, in cereals and grapes. The method was robust, and results for glyphosate, aminomethyl phosphonic acid (AMPA), N-acetyl-AMPA, glufosinate, 3-methylphosphinicopropionic acid (3-MPPA), N-acetyl glufosinate, ethephon, chlorate, perchlorate, fosetyl aluminum, and phosphonic acid at three concentration levels (typically 0.01, 0.05, and 0.1 mg/kg) were compliant with SANTE/11945/2015 guideline method performance criteria. Cereal-based infant food proved to be a more challenging matrix and validated only for glyphosate, chlorate, and perchlorate at 0.005, 0.01, and 0.05 mg/kg. The developed method enables the multiresidue analysis of 12 ionic pesticides and relevant metabolites in a single analysis. Until now, the analysis of these compounds required several different single-residue methods using different chromatographic conditions. This multiresidue approach offers the possibility of more cost-effective and more efficient monitoring of polar ionic pesticides and contaminants that are of concern to food regulation bodies and consumers.
authors.affiliation: Fera Science Ltd. , Sand Hutton, York YO41 1LZ, United Kingdom.
authors.forename: Stuart
authors.identifier: http://orcid.org/0000-0002-8200-9724
authors.initials: S
authors.lastname: Adams
authors.affiliation: Fera Science Ltd. , Sand Hutton, York YO41 1LZ, United Kingdom.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Guest
authors.affiliation: Fera Science Ltd. , Sand Hutton, York YO41 1LZ, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Dickinson
authors.affiliation: Thermo Fisher Scientific , Hemel Hempstead, United Kingdom.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Fussell
authors.affiliation: Thermo Fisher Scientific , San Jose, California, United States.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Beck
authors.affiliation: Special Solutions Center, Thermo Fisher Scientific , Dreieich, Germany.
authors.forename: Frans
authors.initials: F
authors.lastname: Schoutsen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D010573:Pesticide Residues / Q000737:chemistry* / Q000302:isolation & purification; D010575:Pesticides / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010573:Pesticide Residues; D010575:Pesticides
keywords: chlorate; glyphosate; ion chromatography; mass spectrometry; perchlorate