title: Finally, Archaea Get Their CRISPR-Cas Toolbox.
abstract: The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.
authors.forename: Uri
authors.initials: U
authors.lastname: Gophna
authors.affiliation: School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Allers
authors.affiliation: Department of Biology II, Ulm University, 89069 Ulm, Germany. Electronic address: anita.marchfelder@uni-ulm.de.
authors.forename: Anita
authors.initials: A
authors.lastname: Marchfelder
mesh_terms: D001105:Archaea / Q000235:genetics*; D019843:Archaeal Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D059766:DNA End-Joining Repair / Q000235:genetics; D017353:Gene Deletion; D000072669:Gene Editing; D018390:Gene Targeting; D019766:Genes, Archaeal; D059765:Homologous Recombination / Q000235:genetics; D017020:Methanosarcina / Q000235:genetics; D000094704:RNA, Guide, CRISPR-Cas Systems
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D000094704:RNA, Guide, CRISPR-Cas Systems
keywords: CRISPR-Cas; Cas9; HDR; MMEJ; NHEJ; archaea