title: The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
abstract: Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE.Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in them/zrange of 800-10000 Da were analyzed. Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE. Finally, the other 16 MPE and 10 TPE were included to verify the model. We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method.Results. Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS. The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test. The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P= 0.035).Conclusions. The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: National Center of Biomedical Analysis, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Chuanhao
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Weixia
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Tuberculosis, 309 Hospital, PLA, Beijing, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Oncology, General Hospital of the PLA Rocket Force, Beijing, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Gao
authors.affiliation: National Center of Biomedical Analysis, Beijing, China.
authors.forename: Kun
authors.identifier: 0000-0001-6706-8649
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Pulmonary Oncology, 307 Hospital, PLA, Beijing, China.
authors.forename: Xiaoqing
authors.identifier: 0000-0001-7738-109X
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D016022:Case-Control Studies; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism*; D016066:Pleural Effusion, Malignant / Q000378:metabolism* / Q000473:pathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D002272:Carcinoembryonic Antigen; D010455:Peptides