title: Involvement of Epithelial Na+Channel in the Elevated Myogenic Response in Posterior Cerebral Arteries from Spontaneously Hypertensive Rats.
abstract: Hypertension is characterized by increased peripheral vascular resistance which is related with elevated myogenic response. Recent findings have indicated that epithelial sodium channel (ENaC) is involved in mechanotransduction of the myogenic response. The purpose of this study was to investigate the involvement of ENaC in the elevated myogenic response of posterior cerebral arteries (PCAs) from spontaneously hypertensive rats (SHRs). Sixteen to eighteen weeks old male wistar kyoto rats (WKYs) and SHRs were used in this study. We found that wall to lumen (W/L) ratio was increased in the PCAs from SHRs compared with WKYs at the resting state. Interestingly, amiloride significantly inhibited myogenic response in the PCAs from SHRs and WKYs, however, the magnitude of the blockade was greater in SHRs. The transfection of γENaC-siRNA significantly reduced the expression of γENaC protein and inhibited myogenic response in the PCAs from SHRs. Furthermore, these data were supported by the findings that serum/glucocorticoid-induced kinase (Sgk1) and neural precursor cell-expressed developmentally downregulated gene 4-2 (Nedd4-2) were increased in SHRs compared with WKYs. Our results suggest that γENaC may play an important role in the elevated myogenic response in PCAs from SHRs.
authors.affiliation: Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for Medical Sciences, Yonsei University, Seoul, Korea.
authors.forename: Soo-Kyoung
authors.initials: SK
authors.lastname: Choi
authors.affiliation: Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for Medical Sciences, Yonsei University, Seoul, Korea.
authors.forename: Soo-In
authors.initials: SI
authors.lastname: Yeon
authors.affiliation: Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for Medical Sciences, Yonsei University, Seoul, Korea.
authors.forename: Youngin
authors.initials: Y
authors.lastname: Kwon
authors.affiliation: Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for Medical Sciences, Yonsei University, Seoul, Korea.
authors.forename: Seonhee
authors.initials: S
authors.lastname: Byeon
authors.affiliation: Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for Medical Sciences, Yonsei University, Seoul, Korea.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D017874:Immediate-Early Proteins / Q000378:metabolism; D008297:Male; D024510:Muscle Development; D000075702:Nedd4 Ubiquitin Protein Ligases / Q000378:metabolism; D010766:Phosphorylation; D020769:Posterior Cerebral Artery / Q000378:metabolism*; D025341:Principal Component Analysis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D000584:Amiloride; D053503:Epithelial Sodium Channels; D017874:Immediate-Early Proteins; D000075702:Nedd4 Ubiquitin Protein Ligases; D017346:Protein Serine-Threonine Kinases; D021122:Protein Subunits; D034741:RNA, Small Interfering; D000099184:Serum-Glucocorticoid Regulated Kinases; C000618479:NEDD4L protein, rat