title: A secreted chitinase-like protein (OsCLP) supports root growth through calcium signaling in Oryza sativa.
abstract: Chitinases belong to a conserved protein family and play multiple roles in defense, development and growth regulation in plants. Here, we identified a secreted chitinase-like protein, OsCLP, which functions in rice growth. A T-DNA insertion mutant of OsCLP (osclp) showed significant retardation of root and shoot growth. A comparative proteomic analysis was carried out using root tissue of wild-type and the osclp mutant to understand the OsCLP-mediated rice growth retardation. Results obtained revealed that proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1) were differentially regulated in osclp roots. Fura-2 molecular probe staining, which is an intracellular calcium indicator, and inductively coupled plasma-mass spectrometry (ICP-MS) analysis suggested that the intracellular calcium content was significantly lower in roots of osclp as compared with the wild-type. Exogenous application of Ca2+resulted in successful recovery of both primary and lateral root growth in osclp. Moreover, overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected. Taken together, our results highlight the involvement of OsCLP in rice growth by regulating the intracellular calcium concentrations.
authors.affiliation: Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, 660-701, South Korea.
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Institute of Crop Science, Rural Development Administration, Suwon, 16429, South Korea.
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, 446-701, South Korea.
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Jung
authors.affiliation: Department of Plant Bioscience, Pusan National University, Miryang, 627-706, South Korea.|Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, South Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, South Korea.
authors.forename: Joonyup
authors.initials: J
authors.lastname: Kim
authors.affiliation: Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, South Korea.|Department of Horticultural Bioscience, Pusan National University, Miryang, 627-706, South Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, 660-701, South Korea.|National Institute of Crop Science, Rural Development Administration, Suwon, 16429, South Korea.
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
authors.affiliation: Department of Plant Bioscience, Pusan National University, Miryang, 627-706, South Korea.
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D020013:Calcium Signaling; D002688:Chitinases / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology* / Q000254:growth & development; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development*; D040901:Proteomics; D012639:Seeds / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D002688:Chitinases