title: Starter Feeding Supplementation Alters Colonic Mucosal Bacterial Communities and Modulates Mucosal Immune Homeostasis in Newborn Lambs.
abstract: This study aims to investigate the effect of starter feeding supplementation on colonic mucosal bacterial communities and on mucosal immune homeostasis in pre-weaned lambs. We selected eight pairs of 10-day-old lamb twins. One twin was fed breast milk (M,n= 8), while the other was fed breast milk plus starter (M+S,n= 8). The lambs were sacrificed at 56 days age. Colonic content was collected to determine the pH and the concentrations of volatile fatty acids (VFA) and lactate. The colonic mucosa was harvested to characterize the bacterial communities using Illumina MiSeq sequencing and to determine mRNA expression levels of cytokines and toll-like receptors (TLR) using quantitative real-time PCR. The results show that starter feeding decreased luminal pH and increased the concentrations of acetate, propionate, butyrate, total VFA, and lactate in the colon. The principal coordinate analysis (PCA) and analysis of molecular variance show that starter feeding supplementation significantly affected the colonic mucosal bacterial communities with a higher relative abundance of the dominant taxa unclassified S24-7,Oscillibacter, Prevotella, Parabacteroides, Bifidobacterium, Ruminobacter, andSuccinivibrio, and a lower proportion of unclassified Ruminococcaceae,RC9_gut_group, Blautia, Phocaeicola, Phascolarctobacterium, unclassified BS11_gut_group, unclassified family_XIII, andCampylobacterin lambs. Meanwhile, starter feeding decreased mRNA expression of TLR4 and cytokines TNF-α and IFN-γ in colonic tissue. Furthermore, the changes in the colonic mucosal mRNA expression of TLR and cytokines were associated with changes in mucosal bacterial composition. These findings may provide new insights into colonic mucosal bacteria and immune homeostasis in developing lambs.
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University Nanjing, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of NGS Sequencing, Tianyi Health Sciences Institute Zhenjiang, China.
authors.forename: Gaorui
authors.initials: G
authors.lastname: Bian
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University Nanjing, China.
authors.forename: Daming
authors.initials: D
authors.lastname: Sun
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University Nanjing, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University Nanjing, China.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Mao
keywords: bacterial community; colonic mucosa; immune homeostasis; lamb; starter feeding