title: Biocompatible Colloidal Suspensions Based on Magnetic Iron Oxide Nanoparticles: Synthesis, Characterization and Toxicological Profile.
abstract: The use of magnetic iron oxide nanoparticles in biomedicine has evolved intensely in the recent years due to the multiple applications of these nanomaterials, mainly in domains like cancer. The aim of the present study was: (i) to develop biocompatible colloidal suspensions based on magnetic iron oxide nanoparticles as future theranostic tools for skin pathology and (ii) to test their effectsin vitroon human keratinocytes (HaCat cells) andin vivoby employing an animal model of acute dermal toxicity. Biocompatible colloidal suspensions were obtained by coating the magnetic iron oxide nanoparticles resulted during the solution combustion synthesis with a double layer of oleic acid, as innovative procedure in increasing bioavailability. The colloidal suspensions were characterized in terms of dynamic light scattering (DLS) and transmission electron microscopy (TEM). Thein vitroeffects of these suspensions were tested by means of Alamar blue assay and the noxious effects at skin level were measured using non-invasive methods. Thein vitroresults indicated a lack of toxicity on normal human cells induced by the iron oxide nanoparticles colloidal suspensions after an exposure of 24 h to different concentrations (5, 10, and 25 μg·mL-1). The dermal acute toxicity test showed that the topical applications of the colloidal suspensions on female and male SKH-1 hairless mice were not associated with significant changes in the quality of barrier skin function.
authors.affiliation: Faculty of Pharmacy, "Victor Babecs" University of Medicine and Pharmacy Timişoara, Romania.
authors.forename: Dorina-Elena
authors.initials: DE
authors.lastname: Coricovac
authors.affiliation: Faculty of Pharmacy, "Victor Babecs" University of Medicine and Pharmacy Timişoara, Romania.
authors.forename: Elena-Alina
authors.initials: EA
authors.lastname: Moacă
authors.affiliation: Faculty of Pharmacy, "Victor Babecs" University of Medicine and Pharmacy Timişoara, Romania.
authors.forename: Iulia
authors.initials: I
authors.lastname: Pinzaru
authors.affiliation: Faculty of Medicine, "Victor Babeş" University of Medicine and Pharmacy Timişoara, Romania.
authors.forename: Cosmin
authors.initials: C
authors.lastname: Cîtu
authors.affiliation: Faculty of Pharmacy, "Victor Babecs" University of Medicine and Pharmacy Timişoara, Romania.
authors.forename: Codruta
authors.initials: C
authors.lastname: Soica
authors.affiliation: "George Emil Palade" Electron Microscopy Center, Institute of Life Sciences, "Vasile Goldiş" Western University of Arad Arad, Romania.
authors.forename: Ciprian-Valentin
authors.initials: CV
authors.lastname: Mihali
authors.affiliation: Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University of Timişoara Timişoara, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Păcurariu
authors.affiliation: Federal Research Centre of Nutrition, Biotechnology and Food Safety Moscow, Russia.
authors.forename: Victor A
authors.initials: VA
authors.lastname: Tutelyan
authors.affiliation: Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete Crete, Greece.
authors.forename: Aristidis
authors.initials: A
authors.lastname: Tsatsakis
authors.affiliation: Faculty of Pharmacy, "Victor Babecs" University of Medicine and Pharmacy Timişoara, Romania.
authors.forename: Cristina-Adriana
authors.initials: CA
authors.lastname: Dehelean
keywords: bioavailability; colloidal suspensions; magnetic iron oxide nanoparticles; skin barrier; solution combustion synthesis; toxicity