title: Enhanced immunogenicity following miR-155 incorporation into the influenza A virus genome.
abstract: Influenza A vaccine efficacy in the elderly is generally poor and so identification of novel molecular adjuvants to improve immunogenicity is important to reduce the overall burden of disease. Short non-coding RNAs, known as microRNAs (miRNAs) are known to regulate gene expression and have the potential to influence immune responses. One such miRNA, miR-155, has been shown to modulate T and B cell development and function. We incorporated miR-155 into the influenza A virus (IAV) genome creating a self-adjuvanting 'live vaccine' with the ability to modify immunogenicity. Infection of mice with a recombinant influenza virus encoding miR-155 in the NS gene segment altered epitope-specific expansion of influenza-specific CD8+T cells and induced significantly higher levels of neutralising antibody.
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, Australia; Australian Animal Health Laboratory, CSIRO, Geelong, VIC, Australia.
authors.forename: Leonard
authors.initials: L
authors.lastname: Izzard
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, Australia; Australian Animal Health Laboratory, CSIRO, Geelong, VIC, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Dlugolenski
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, Australia; Australian Animal Health Laboratory, CSIRO, Geelong, VIC, Australia.
authors.forename: Yingju
authors.initials: Y
authors.lastname: Xia
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, Australia; Australian Animal Health Laboratory, CSIRO, Geelong, VIC, Australia.
authors.forename: Meagan
authors.initials: M
authors.lastname: McMahon
authors.affiliation: Australian Animal Health Laboratory, CSIRO, Geelong, VIC, Australia.
authors.forename: Deborah
authors.initials: D
authors.lastname: Middleton
authors.affiliation: University of Georgia, College of Veterinary Medicine, Department of Infectious Diseases, Athens, GA, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Tripp
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, Australia; Australian Animal Health Laboratory, CSIRO, Geelong, VIC, Australia. Electronic address: john.stambas@deakin.edu.au.
authors.forename: John
authors.initials: J
authors.lastname: Stambas
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016679:Genome, Viral; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; C108309:NS protein, influenza virus; D017361:Viral Nonstructural Proteins
keywords: B cell; Influenza A; RNAi; T cell; Vaccine; miR-155