title: Cross-Validation of High-Resolution Melting Analysis-Based Genotyping Platform.
abstract: OBJECTIVE
  Developing genetic and pharmacogenetic panels enhances genetic testing in clinical molecular diagnostics and precision medicine. This study was designed to cross-validate the performance of Canon's multiplex high-resolution DNA melting analysis platform with the Applied Biosystems TaqMan®-based Quant Studio Real-Time PCR System and Pyrosequencing®genotyping platforms for common genetic polymorphisms of the vitamin K epoxide reductase complex 1 (VKORC1) and CYP2C9.
  
  
  METHODS
  Genomic DNA isolated from 240 blood and saliva samples was used to genotype the VKORC1-1639 G/A (rs9923231), CYP2C9*2 (430C>T, rs28371674), and CYP2C9*3 (1075A>C, rs1057910) single-nucleotide polymorphisms (SNPs) on the three above-mentioned genotyping platforms.
  
  
  RESULTS
  There was 99.2%, 100%, and 100% concordance among the Canon DNA analyzer, the TaqMan-based QuantStudio, and the Pyrosequencing genotyping results for the VKORC1 (rs9923231), CYP2C9*2, and CYP2C9*3 SNPs, respectively, in DNA samples isolated from blood. The DNA samples isolated from saliva showed 100% concordance among the three test platforms for the three tested SNPs.
  
  
  CONCLUSIONS
  These results show that, the DNA analyzer performed very well when compared with two commonly used genotyping platforms. The reliability, multiple genetic variant testing capability, and short turnaround time for up to eight samples make the DNA analyzer an ideal genotyping platform for genetic testing in the clinical practice setting, where efficient genotyping is important to prevent delays in optimizing drug therapy.
authors.affiliation: Department of Pharmacotherapy and Translational Research, Center for Pharmacogenomics, University of Florida , College of Pharmacy, Gainesville, Florida.
authors.forename: Taimour
authors.initials: T
authors.lastname: Langaee
authors.affiliation: Department of Pharmacotherapy and Translational Research, Center for Pharmacogenomics, University of Florida , College of Pharmacy, Gainesville, Florida.
authors.forename: Lynda
authors.initials: L
authors.lastname: Stauffer
authors.affiliation: Department of Pharmacotherapy and Translational Research, Center for Pharmacogenomics, University of Florida , College of Pharmacy, Gainesville, Florida.
authors.forename: Cheryl
authors.initials: C
authors.lastname: Galloway
authors.affiliation: Department of Pharmacotherapy and Translational Research, Center for Pharmacogenomics, University of Florida , College of Pharmacy, Gainesville, Florida.
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Solayman
authors.affiliation: Department of Pharmacotherapy and Translational Research, Center for Pharmacogenomics, University of Florida , College of Pharmacy, Gainesville, Florida.
authors.forename: Larisa
authors.initials: L
authors.lastname: Cavallari
mesh_terms: D065729:Cytochrome P-450 CYP2C9 / Q000032:analysis / Q000097:blood / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009691:Nucleic Acid Denaturation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012463:Saliva; D064417:Vitamin K Epoxide Reductases / Q000032:analysis / Q000097:blood / Q000235:genetics*
chemical_list: C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; C578523:VKORC1 protein, human; D064417:Vitamin K Epoxide Reductases
keywords: high-resolution melting analysis; microfluidic; rapid serial PCR