title: A Novel Prosthetic Joint Infection Pathogen, Mycoplasma salivarium, Identified by Metagenomic Shotgun Sequencing.
abstract: Defining the microbial etiology of culture-negative prosthetic joint infection (PJI) can be challenging. Metagenomic shotgun sequencing is a new tool to identify organisms undetected by conventional methods. We present a case where metagenomics was used to identify Mycoplasma salivarium as a novel PJI pathogen in a patient with hypogammaglobulinemia.
authors.affiliation: Division of Infectious Diseases, Department of Medicine.
authors.forename: Matthew
authors.initials: M
authors.lastname: Thoendel
authors.affiliation: Department of Surgery and Center for Individualized Medicine.
authors.forename: Patricio
authors.initials: P
authors.lastname: Jeraldo
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology.
authors.forename: Kerryl E
authors.initials: KE
authors.lastname: Greenwood-Quaintance
authors.affiliation: Department of Surgery and Center for Individualized Medicine.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Chia
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Abdel
authors.affiliation: Division of Infectious Diseases, Department of Medicine.
authors.forename: James M
authors.initials: JM
authors.lastname: Steckelberg
authors.affiliation: Division of Infectious Diseases, Department of Medicine.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Osmon
authors.affiliation: Division of Infectious Diseases, Department of Medicine.|Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology.
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D000361:Agammaglobulinemia / Q000150:complications / Q000382:microbiology; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007595:Joint Prosthesis / Q000382:microbiology*; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D045804:Mycoplasma salivarium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016459:Prosthesis-Related Infections / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology*
keywords: Mycoplasma; metagenomics; prosthetic joint infection