title: Selective dysfunction of subsets of peripheral blood mononuclear cells during pediatric dengue and its relationship with clinical outcome.
abstract: During dengue virus (DENV) infection, a blockage of secretion of cytokines such as tumor necrosis factor (TNF)-α and members of the interferon (IFN) family has been described in vitro. We evaluated the functionality of monocytes as well as dendritic, B and T cells isolated from children with mild and severe dengue. Compared with those of healthy children, stimulated monocytes, CD4+T cells and dendritic cells from children with dengue had lower production of proinflammatory cytokines. The interferon axis was dramatically modulated by infection as plasmacytoid dendritic cells (pDCs) and CD4+T cells had low production of IFN-α and IFN-γ, respectively; plasma levels of IFN-α and IFN-γ were lower in severely ill children, suggesting a protective role. Patients with antigenemia had the highest levels of IFN-α in plasma but the lowest frequency of IFN-α-producing pDCs, suggesting that DENV infection stimulates a systemic type I IFN response but affects the pDCs function.
authors.affiliation: Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia.
authors.forename: Federico
authors.initials: F
authors.lastname: Perdomo-Celis
authors.affiliation: Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia; Servicio de Pediatría, Hospital Universitario de Neiva, Neiva, Colombia.
authors.forename: Doris M
authors.initials: DM
authors.lastname: Salgado
authors.affiliation: Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia. Electronic address: cfnarvaez@usco.edu.co.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Narváez
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology*; D005260:Female; D006801:Humans; D007223:Infant; D016898:Interferon-alpha / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology; D010372:Pediatrics / Q000706:statistics & numerical data; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D016898:Interferon-alpha; D014409:Tumor Necrosis Factor-alpha
keywords: Cytokine; Dengue; Interferon; Monocytes; Plasmacytoid dendritic cell; T cells