title: Treatment with adipose derived mesenchymal stem cells and their conditioned media reverse carrageenan induced paw oedema in db/db mice.
abstract: Mesenchymal stem cells are known for anti inflammatory and immunomodulatory activities. The aim of our study was to evaluate the effect of human adipose derived mesenchymal stem cells (hADMSCs) and its conditioned media (CM) on carrageenan induced acute inflammation in db/db mice. We injected 5×105ADMSCs or the CM in the inflamed paw. We assessed the paw volume, serum IL6 levels and histopathology of the paw to reveal the anti inflammatory effect. We observed a single injection of hADMSCs or CM could reverse the inflammation within 24h as evidenced by reduction in paw volume, IL6 levels and histological examination. Our result equivocally demonstrates the role of CM in normalising the inflammation better than hADMSCs. This study will pave way for an alternative to anti inflammatory drugs.
authors.affiliation: School of Regenerative Medicine, GKVK Post, Bellary Road, Bangalore 560065, India.
authors.forename: Nitya
authors.initials: N
authors.lastname: Shree
authors.affiliation: Department of Biotechnology, Jain University, Bangalore, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Venkategowda
authors.affiliation: Connexios Life Sciences Pvt. Ltd., Bangalore, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Venkatranganna
authors.affiliation: School of Regenerative Medicine, GKVK Post, Bellary Road, Bangalore 560065, India. Electronic address: rr.bhonde@manipal.edu.
authors.forename: Ramesh R
authors.initials: RR
authors.lastname: Bhonde
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D050154:Adiposity / Q000502:physiology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002351:Carrageenan / Q000494:pharmacology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D015850:Interleukin-6 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D000893:Anti-Inflammatory Agents; D017077:Culture Media, Conditioned; D015850:Interleukin-6; D002351:Carrageenan
keywords: Carrageenan; Conditioned media; Inflammation; Oedema; hADMSCs