title: Selected life-extending interventions reduce arterial CXCL10 and macrophage colony-stimulating factor in aged mouse arteries.
abstract: Cardiovascular disease (CVD) is the leading cause of death in the industrialized world. Aging is the most predictive risk factor for CVD and is associated with arterial inflammation which contributes to increased CVD risk. Although age-related arterial inflammation has been described in both humans and animals, only a limited number of inflammatory mediators, cytokines and chemokines have been identified. In this investigation we sought to determine whether lifespan extending interventions, including crowded litter early life nutrient deprivation (CL), traditional lifelong caloric restriction (CR) and lifelong Rapamycin treatment (Rap) would attenuate age-related arterial inflammation using multi analyte profiling. Aortas from Young (4-6months), Old (22months), Old CL, Old CR and Old Rap mice were homogenized and cytokine concentrations were assessed using Luminex Multi Analyte Profiling. Chemokines involved in immune cell recruitment, such as CCL2, CXCL9, CXCL10, GMCSF and MCSF, were increased in Old vs. Young (p<0.05). The age-related increase of CXCL10 was prevented by CR (p<0.05 vs. Old). MSCF concentrations were lower in aortas of Rap treated mice (p<0.05 vs. Old). Interleukins (IL), IL-1α, IL-1β and IL-10, were also greater in Old vs. Young mice (p<0.05). These data demonstrate selected lifespan extending interventions can prevent or limit age-related increases in selected aortic chemokines.
authors.affiliation: University of Utah, Department of Internal Medicine, Division of Geriatrics, Salt Lake City, UT, USA. Electronic address: daniel.trott@utah.edu.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Trott
authors.affiliation: University of Utah, Department of Internal Medicine, Division of Geriatrics, Salt Lake City, UT, USA; University of Utah, Department of Nutrition and Integrative Physiology, Salt Lake City, UT, USA; University of Utah, Department of Biochemistry, Salt Lake City, UT, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Lesniewski
authors.affiliation: University of Utah, Department of Internal Medicine, Division of Geriatrics, Salt Lake City, UT, USA; University of Utah, Department of Nutrition and Integrative Physiology, Salt Lake City, UT, USA; Veterans Affairs Medical Center, Geriatric Research, Education, and Clinical Center, Salt Lake City, UT, USA; University of Utah, Department of Biochemistry, Salt Lake City, UT, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Donato
mesh_terms: D000375:Aging; D000818:Animals; D001158:Arteries / Q000276:immunology*; D031204:Caloric Restriction; D002318:Cardiovascular Diseases / Q000188:drug therapy / Q000517:prevention & control; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054407:Chemokine CCL4 / Q000096:biosynthesis; D054357:Chemokine CXCL10 / Q000097:blood* / Q000276:immunology; D018925:Chemokines / Q000096:biosynthesis / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D061345:Early Medical Intervention; D016753:Interleukin-10 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000097:blood; D007378:Interleukins / Q000096:biosynthesis / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood* / Q000276:immunology; D008297:Male; D051379:Mice; D020123:Sirolimus / Q000627:therapeutic use
chemical_list: D018932:Chemokine CCL2; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D018925:Chemokines; C485433:Cxcl10 protein, mouse; D016207:Cytokines; D053583:Interleukin-1beta; D007378:Interleukins; D016753:Interleukin-10; D016173:Macrophage Colony-Stimulating Factor; D020123:Sirolimus
keywords: Aorta; Caloric restriction; Chemokine; Cytokine; Interleukin; Rapamycin