title: Induction of EROD and BFCOD activities in tissues of barbel (Barbus callensis) from a water reservoir in Algeria.
abstract: EROD and BFCOD activities were measured in liver and gills of barbel (Barbus callensis, a native North African species) captured at Beni Haroun lake, the most important water reservoir in Algeria. This lake receives wastewater from different origins. Thus, we assessed the level of pollution through the induction of detoxification activities in tissues of barbel, evaluating simultaneously the suitability of this species to be used as a sentinel. Fish were collected between March 2015 and January 2016 at three locations taking into account the pollution sources and accessibility. In liver, EROD and BFCOD showed the highest induction in October specially in the location of the dam that received pollutants. In gills, only EROD, but not BFCOD, activity was detected. Maximal EROD induction was noted in samples from January. Fish cell lines (RTG-2 and PLHC-1) were exposed to sediments extracts collected at Beni Haroun lake and enzyme activities (EROD and BFCOD, respectively) were measured. Sediment extracts did not induce BFCOD activity. The EROD induction observed in RTG-2 cells was in line with the results observed in fish tissues. Our results suggest that the lake is at risk from pollution and that Barbus callensis is a good sentinel species.
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Crta. de la Coruña, km. 7, 5, 28040 Madrid, Spain; Département d'Ecologie et Génie de l'Environnement., Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre et de l'Univers, Université 08, Mai 1945 Guelma, BP 401, 24000 Guelma, Algeria; Laboratoire de Biotechnologie, Environnement et Santé, Université Mohammed Seddik Benyahya, Jijel, BP 98, Ouled Aissa, Jijel 18000, Algeria.
authors.forename: Safia
authors.initials: S
authors.lastname: Habila
authors.affiliation: Laboratoire de Biotechnologie, Environnement et Santé, Université Mohammed Seddik Benyahya, Jijel, BP 98, Ouled Aissa, Jijel 18000, Algeria.
authors.forename: Essaid
authors.initials: E
authors.lastname: Leghouchi
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Crta. de la Coruña, km. 7, 5, 28040 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Valdehita
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Crta. de la Coruña, km. 7, 5, 28040 Madrid, Spain.
authors.forename: Azucena
authors.initials: A
authors.lastname: Bermejo-Nogales
authors.affiliation: Laboratoire de Pharmacologie et Phytochimie, Université Mohammed Seddik Benyahya, Jijel, BP 98, Ouled Aissa, Jijel 18000, Algeria.
authors.forename: Smail
authors.initials: S
authors.lastname: Khelili
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Crta. de la Coruña, km. 7, 5, 28040 Madrid, Spain. Electronic address: jmnavas@inia.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Navas
mesh_terms: D000462:Algeria; D000818:Animals; D002460:Cell Line; D003530:Cyprinidae / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004784:Environmental Monitoring; D005880:Gills / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D060106:Lakes / Q000737:chemistry*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D012621:Seasons; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1
keywords: Biomarkers; Fish; POPs; Pollution; Sediments