title: Dynamic Measures of Flagellar Gene Expression.
abstract: Many genes are required to assemble flagella. These genes encode not only the structural elements of the flagellum but also a number of regulators that control how the flagellar genes are temporally expressed during the assembly process. These regulators also specify the likelihood that a given cell will express the flagellar genes. In particular, not all cells express the flagellar genes, resulting in mixed populations of motile and non-motile cells. Nutrients provide one signal that specifies the motile fraction. In this chapter, we describe two methods for measuring flagellar gene expression dynamics using fluorescent proteins in Salmonella enterica. Both the methods can be used to investigate the mechanisms governing flagellar gene expression dynamics.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Koirala
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA. cvrao@illinois.edu.
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Rao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D005407:Flagella / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics
chemical_list: D001426:Bacterial Proteins
keywords: Flagella; Flow cytometry; Fluorescence; Gene expression; Salmonella