title: LMD proteomics provides evidence for hippocampus field-specific motor protein abundance changes with relevance to Alzheimer's disease.
abstract: Human hippocampal area Cornu Ammonis (CA) 1 is one of the first fields in the human telencephalon showing Alzheimer disease (AD)-specific neuropathological changes. In contrast, CA2 and CA3 are far later affected pointing to functional differences, which may be accompanied by differences in proteome endowment and changes.
  
  
  
  Human pyramidal cell layers of hippocampal areas CA1, CA2, and CA3 from neurologically unaffected individuals were excised using laser microdissection. The proteome of each individual sample was analyzed and differentially abundant proteins were validated by immuno-histochemistry.
  
  
  
  Comparison of CA1 to CA2 revealed 223, CA1 to CA3 197 proteins with differential abundance, among them we found motor proteins MYO5A and DYNC1H1. Extension of the study to human hippocampus slices from AD patients revealed extensive depletion of these proteins in CA1 area compared to unaffected controls.
  
  
  
  High abundance of motor proteins in pyramidal cell layers CA1 compared to CA2 and CA3 points the specific vulnerability of this hippocampal area to transport-associated changes based on microtubule dysfunction and destabilization in AD.
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Biomedical Research, Dortmund, Germany; Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Human Brain Proteomics I, Dortmund, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schrötter
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Biomedical Research, Dortmund, Germany; Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Human Brain Proteomics I, Dortmund, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Oberhaus
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Dortmund, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Kolbe
authors.affiliation: Chair of Plant Biochemistry, Ruhr University Bochum, Bochum, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Seger
authors.affiliation: Institute of Biochemistry and Pathobiochemistry, Ruhr University Bochum, Bochum, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mastalski
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Biomedical Research, Dortmund, Germany.
authors.forename: Fouzi
authors.initials: F
authors.lastname: El Magraoui
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Biomedical Research, Dortmund, Germany.
authors.forename: Edeltraut
authors.initials: E
authors.lastname: Hoffmann-Posorske
authors.affiliation: Institut of Legal Medicine, Julius-Maximilians-University Würzburg, Würzburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bohnert
authors.affiliation: Center of Mental Health, Department of Psychiatry, Psychosomatics and Psychotherapy, University of Würzburg, Füchsleinstrasse 15, 97080 Würzburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Deckert
authors.affiliation: Institute of Legal Medicine and Forensic Sciences, Ludwig-Maximilians-University Munich, Munich, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Braun
authors.affiliation: Institute of Legal Medicine and Forensic Sciences, Ludwig-Maximilians-University Munich, Munich, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Graw
authors.affiliation: Department of Neuroanatomy, Ludwig-Maximilians University of Munich Munich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Schmitz
authors.affiliation: Center for Neuropathology and Prion Research, Ludwig-Maximilians-University Munich, Munich D-81377,Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Arzberger
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Dortmund, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Loosse
authors.affiliation: Department of Psychiatry, Psychiatric Clinic, Julius-Maximilians-University Würzburg, Germany; Department of Pathology, LIM 22, University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Helmut
authors.initials: H
authors.lastname: Heinsen
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Biomedical Research, Dortmund, Germany. Electronic address: helmut.e.meyer@isas.de.
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Dortmund, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Müller
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D002853:Chromatography, Liquid; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D009419:Nerve Tissue Proteins
keywords: Alzheimer's disease; Hippocampus; Immunohistochemistry; Laser microdissection; Proteomics