title: Sequestration of Guest Intermediates by Dalesconol Bioassembly Lines in Daldinia eschscholzii.
abstract: Microbial constructions of secondary metabolites are generally biosynthetic gene cluster (BGC)-based, and the forging of different BGC-sourced intermediates tends to be overlooked. Here, we show that the dalesconol bioassembly lines in Daldinia eschscholzii can sequester guest intermediates (i.e., building blocks produced outside the dalesconol biosynthetic gene cluster) to form arrays of skeletally undescribed molecules such as (+)-dalescone A, a potent inhibitor against the NLRP3 inflammasome activation.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University , Nanjing 210046, China.
authors.forename: Ai Hua
authors.initials: AH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University , Nanjing 210046, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Nanjing Medical University , Nanjing 210029, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University , Nanjing 210046, China.
authors.forename: Xin Lei
authors.initials: XL
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University , Nanjing 210046, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University , Nanjing 210046, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University , Nanjing 210046, China.|State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine , Nanjing 210023, China.
authors.forename: Ren Xiang
authors.identifier: 0000-0001-6532-6261
authors.initials: RX
authors.lastname: Tan
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D053898:Biosynthetic Pathways; D002460:Cell Line; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D005810:Multigene Family; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry* / Q000378:metabolism; D064210:Secondary Metabolism; D013237:Stereoisomerism; D013098:Xylariales / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001688:Biological Products; D058847:Inflammasomes; D053583:Interleukin-1beta; D011084:Polycyclic Aromatic Hydrocarbons; C552720:dalesconol A