title: Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface.
abstract: Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate vascular functions as well as homeostasis at the vascular interface. To date, the astroglial mechanisms underlying these functions have been poorly addressed. Here we demonstrate that a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, a pool of messenger RNAs bound to ribosomes, the endfeetome, that primarily encodes for secreted and membrane proteins. We detected nascent protein synthesis in astrocyte endfeet. Finally, we determined the presence of smooth and rough endoplasmic reticulum and the Golgi apparatus in astrocyte perivascular processes and endfeet, suggesting for local maturation of membrane and secreted proteins. These results demonstrate for the first time that protein synthesis occurs in astrocyte perivascular distal processes that may sustain their structural and functional polarization at the vascular interface.
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology/Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique CNRS, UMR 7241/Institut National de la Santé et de la Recherche Médicale INSERM, U1050/University Pierre et Marie Curie UPMC, ED, No. 158/MEMOLIFE Laboratory of Excellence and PSL Research University, Paris, France; Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité, Paris, France.
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Boulay
authors.affiliation: Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité, Paris, France; Cellular and Molecular Imaging Facility, INSERM US25/CNRS UMS 3612/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Sorbonne Paris Cité, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Saubaméa
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology/Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique CNRS, UMR 7241/Institut National de la Santé et de la Recherche Médicale INSERM, U1050/University Pierre et Marie Curie UPMC, ED, No. 158/MEMOLIFE Laboratory of Excellence and PSL Research University, Paris, France; Institut Pasteur, Human Histopathology and Animal Models, Paris, France; Université Paris Descartes-Sorbonne Paris Cité, Paris, France; Service de Neuropathologie, Centre Hospitalier Sainte-Anne, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Adam
authors.affiliation: Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité , Paris, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Chasseigneaux
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology/Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique CNRS, UMR 7241/Institut National de la Santé et de la Recherche Médicale INSERM, U1050/University Pierre et Marie Curie UPMC, ED, No. 158/MEMOLIFE Laboratory of Excellence and PSL Research University , Paris, France.
authors.forename: Noémie
authors.initials: N
authors.lastname: Mazaré
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology/Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique CNRS, UMR 7241/Institut National de la Santé et de la Recherche Médicale INSERM, U1050/University Pierre et Marie Curie UPMC, ED, No. 158/MEMOLIFE Laboratory of Excellence and PSL Research University , Paris, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Gilbert
authors.affiliation: Plateforme Bioinformatique, Ecole Normale Supérieure/Institut de Biologie de l'ENS (IBENS) /INSERM, U1024/CNRS, UMR 8197 , Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Bahin
authors.affiliation: Plateforme Bioinformatique, Ecole Normale Supérieure/Institut de Biologie de l'ENS (IBENS) /INSERM, U1024/CNRS, UMR 8197 , Paris, France.
authors.forename: Leïla
authors.initials: L
authors.lastname: Bastianelli
authors.affiliation: Plateforme Génomique, Ecole Normale Supérieure/PSL Research University/IBENS/INSERM/CNRS , Paris, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Blugeon
authors.affiliation: Plateforme Génomique, Ecole Normale Supérieure/PSL Research University/IBENS/INSERM/CNRS , Paris, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Perrin
authors.affiliation: Plateforme de qPCR à Haut Débit, Ecole Normale Supérieure/IBENS , Paris, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Pouch
authors.affiliation: Plateforme de qPCR à Haut Débit, Ecole Normale Supérieure/IBENS, Paris, France; Laboratoire de Physique Statistique, ENS/CNRS UMR 8550/PSL Research University/Université Paris Diderot Sorbonne Paris-Cité/Sorbonne Universités UPMC, Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Ducos
authors.affiliation: Plateforme Génomique, Ecole Normale Supérieure/PSL Research University/IBENS/INSERM/CNRS, Paris, France; Evolution Paris Seine, Institut de Biologie Paris-Seine (IBPS)/Sorbonne Universités, UPMC/CNRS UMR 7138, Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Le Crom
authors.affiliation: Plateforme Bioinformatique, Ecole Normale Supérieure/Institut de Biologie de l'ENS (IBENS) /INSERM, U1024/CNRS, UMR 8197 , Paris, France.
authors.forename: Auguste
authors.initials: A
authors.lastname: Genovesio
authors.affiliation: Institut Pasteur, Human Histopathology and Animal Models, Paris, France; Université Paris Descartes-Sorbonne Paris Cité, Paris, France; Service de Neuropathologie, Centre Hospitalier Sainte-Anne, Paris, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chrétien
authors.affiliation: Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité , Paris, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Declèves
authors.affiliation: Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité , Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Laplanche
authors.affiliation: Neuroglial Interactions in Cerebral Physiopathology/Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique CNRS, UMR 7241/Institut National de la Santé et de la Recherche Médicale INSERM, U1050/University Pierre et Marie Curie UPMC, ED, No. 158/MEMOLIFE Laboratory of Excellence and PSL Research University , Paris, France.
authors.forename: Martine
authors.identifier: 0000-0002-5312-8476
authors.initials: M
authors.lastname: Cohen-Salmon
keywords: astrocyte; endfeet; gliovascular unit; local translation; mRNAs localization; translating ribosome immunoprecipitation