title: A novel mitochondria-targeted near-infrared fluorescence probe for ultrafast and ratiometric detection of SO2derivatives in live cells.
abstract: A novel mitochondria-targeted ratiometric near-infrared fluorescence probe NDMBT for Sulfur dioxide (SO2) derivatives was constructed based on the SO2derivatives-triggered Michael addition reaction. It displayed ultrafast response time (within 10s), large hypsochromic shift (260nm), high photostability, excellent selectivity and high sensitivity in aqueous media with a detection limit of 43nM. More importantly, it was successfully applied to imaging of the enzymatically generated SO2derivatives in mitochondria of live cells.
authors.affiliation: Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: wendao__wei@hnu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Xian-Jun
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: yqkuang@hnu.edu.cn.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Kuang
authors.affiliation: Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D049109:Cell Proliferation; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D057230:Limit of Detection; D008928:Mitochondria / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D013458:Sulfur Dioxide / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013458:Sulfur Dioxide
keywords: Mitochondria-targeted; Near-infrared fluorescence probe; Ratiometric fluorescence detection; SO(2) derivatives