title: Isolation of fucoidan from Sargassum polycystum brown algae: Structural characterization, in vitro antioxidant and anticancer activity.
abstract: In this study antioxidant and anticancer effect of fucoidan isolated from brown seaweed Sargassum polycystum was investigated. The total yield of fucoidan was 4.51±0.24%, of these, 46.8% of fucose and 22.35±0.23% of sulphate respectively. The structural characteristic of fucoidan was analyzed by Fourier transform infrared spectroscopy and nuclear magnetic resonance. The antioxidant properties were determined by DPPH scavenging, reducing power and total antioxidant assays. The maximum DPPH scavenging activity (61.2±0.33%), reducing ability (67.56±0.26%) and total antioxidant activity (65.3±0.66%) were obtained at 1000μg/ml of fucoidan. The cytotoxicity effect of fucoidan showed a higher percentage (90.4±0.25%) of inhibition against the MCF-7 cell line at 150μg/ml with an estimated IC50at 50μg/ml. Further, cytomorphological and apoptosis changes of fucoidan treated cells were observed under inverted light microscope and confocal laser scanning microscope (CLSM). The results demonstrated that the isolated fucoidan from S. polycystum possessed potent antioxidant and anticancer properties.
authors.affiliation: Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, India.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Palanisamy
authors.affiliation: Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, India.
authors.forename: Manoharan
authors.initials: M
authors.lastname: Vinosha
authors.affiliation: Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Chennai 600 119, India; Hi-Tech Vet Pharma, Animal Health Solution, Research & Development, Brindavanam, Nellore - 524 001, A.P, India.
authors.forename: Thangapandi
authors.initials: T
authors.lastname: Marudhupandi
authors.affiliation: Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, India.
authors.forename: Periyannan
authors.initials: P
authors.lastname: Rajasekar
authors.affiliation: Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, India. Electronic address: 71gnmprabhu@gmail.com.
authors.forename: Narayanan Marimuthu
authors.initials: NM
authors.lastname: Prabhu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival; D006801:Humans; D061986:MCF-7 Cells; D000459:Phaeophyceae / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; C007789:fucoidan; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Anticancer activity; Antioxidant activity; Brown seaweed; Fucoidan; Sargassum polycystum