title: High-efficient production of citric acid by Aspergillus niger from high concentration of substrate based on the staged-addition glucoamylase strategy.
abstract: Citric acid (CA), an important platform-compound, has attracted much attention because of its broad applications and huge market demand. To solve high residual sugar at the fermentation end, we put forwarded strategy of pre-saccharification and then fermentation. Results showed that the residual total sugar decreased by 10.4% and the productivity increased by 4.0% and initially high glucose inhibited cell growth. Furthermore, commercial glucoamylase with high low-pH stability was proposed to staged-add in the fermentation process, which timely compensated enzyme loss, ensuring the glucose supply rate. The fermentation productivity was evidently enhanced by 13.3% with residual total sugar decreasing by 31.3%, simplifying the subsequent product separation and extraction process. Our results confirmed that staged-addition glucoamylase strategy was feasible to effective production of CA.
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China.|School of Biotechology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Jiangsu Guoxin Union Energy Co., Ltd., Wuxi, 214203, People's Republic of China.
authors.forename: Baoshi
authors.initials: B
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China.|School of Biotechology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Linghuan
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Jiangsu Guoxin Union Energy Co., Ltd., Wuxi, 214203, People's Republic of China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Tan
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China.|School of Biotechology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Youran
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China.|School of Biotechology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China.|School of Biotechology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Ding
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Wuxi, 214122, People's Republic of China. gyshi@jiangnan.edu.cn.|School of Biotechology, Jiangnan University, Wuxi, 214122, People's Republic of China. gyshi@jiangnan.edu.cn.
authors.forename: Guiyang
authors.initials: G
authors.lastname: Shi
mesh_terms: D001234:Aspergillus niger; D019343:Citric Acid; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase; D013379:Substrate Specificity
chemical_list: D019343:Citric Acid; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Aspergillus niger; Citric acid; Glucoamylase; Simultaneous saccharification and fermentation; Staged-addition strategy