title: Genome-Wide Identification, Expression Patterns, and Functional Analysis of UDP Glycosyltransferase Family in Peach (Prunus persicaL. Batsch).
abstract: Peach (Prunus persicaL. Batsch) is a commercial grown fruit trees, important because of its essential nutrients and flavor promoting secondary metabolites. The glycosylation processes mediated by UDP-glycosyltransferases (UGTs) play an important role in regulating secondary metabolites availability. Identification and characterization of peachUGTsis therefore a research priority. A total of 168 peachUGTgenes that distributed unevenly across chromosomes were identified based on their conserved PSPG motifs. Phylogenetic analysis of these genes with plant UGTs clustered them into 16 groups (A-P). Comparison of the patterns of intron-extron and their positions within genes revealed one highly conserved intron insertion event in peachUGTs. Tissue specificity, temporal expression patterns in peach fruit during development and ripening, and in response to abiotic stress UV-B irradiation was investigated using RNA-seq strategy. The relationship betweenUGTstranscript levels and concentrations of glycosylated volatiles was examined to select candidates for functional analysis. Heterologous expressing these candidate genes inEscherichia coliidentified UGTs that were involved in thein vitrovolatile glycosylation. Our results provide an important source for the identification of functionalUGTgenes to potential manipulate secondary biosynthesis in peach.
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Boping
authors.initials: B
authors.lastname: Wu
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Liuxiao
authors.initials: L
authors.lastname: Gao
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Yaying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Hongru
authors.initials: H
authors.lastname: Liu
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Cao
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology/Laboratory of Fruit Quality Biology, Zhejiang University Hangzhou, China.
authors.forename: Kunsong
authors.initials: K
authors.lastname: Chen
keywords: UGT; expression pattern; peach; phylogeny; secondary metabolites