title: Enhanced Self-Renewal and Accelerated Differentiation of Human Fetal Neural Stem Cells Using Graphene Oxide Nanoparticles.
abstract: Graphene oxide (GO) has received increasing attention in bioengineering fields due to its unique biophysical and electrical properties, along with excellent biocompatibility. The application of GO nanoparticles (GO-NPs) to engineer self-renewal and differentiation of human fetal neural stem cells (hfNSCs) is reported. GO-NPs added to hfNSC culture during neurosphere formation substantially promote cell-to-cell and cell-to-matrix interactions in neurospheres. Accordingly, GO-NP-treated hfNSCs show enhanced self-renewal ability and accelerated differentiation compared to untreated cells, indicating the utility of GO in developing stem cell therapies for neurogenesis.
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
authors.forename: Kisuk
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, and Institute of Nano Science and Technology (INST), Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Yong Hwa
authors.initials: YH
authors.lastname: Hwang
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
authors.forename: Hyun-Ji
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Severance Children's Hospital, Department of Pediatrics and BK21 Plus Project for Medical Sciences, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.
authors.forename: Kook In
authors.initials: KI
authors.lastname: Park
authors.affiliation: Department of Bioengineering, College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, and Institute of Nano Science and Technology (INST), Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Dong Yun
authors.initials: DY
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
mesh_terms: D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects*; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism*
chemical_list: D006108:Graphite
keywords: differentiation; graphene oxide nanoparticle; neural stem cell; self-renewal