title: Both PepT1 and GLUT Intestinal Transporters Are Utilized by a Novel Glycopeptide Pro-Hyp-CONH-GlcN.
abstract: Pro-Hyp (PO) accounts for many beneficial biological effects of collagen hydrolysates for skin and bone health. The objective of this study was to conjugate PO with glucosamine (GlcN) to create a novel glycopeptide Pro-Hyp-CONH-GlcN (POGlcN) and then to investigate the potential involvement of multiple transepithelial transport pathways for this glycopeptide. Nuclear magnetic resonance results revealed the amide nature of this glycopeptide with α and β configurations derived from GlcN. This glycopeptide was very resistant to simulated gastrointestinal digestion. Also, it showed a rate of transepithelial transport [permeability coefficient (Papp) of (2.82 ± 0.15) × 10-6cm/s] across the Caco-2 cell monolayer superior to those of parental dipeptide PO and GlcN [Pappvalues of (1.45 ± 0.17) × 10-6and (1.87 ± 0.15) × 10-6cm/s, respectively]. A transport mechanism experiment indicated that the improved transport efficiency of POGlcN is attributed to the introduction of glucose transporters.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta , 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta , 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.
authors.forename: Mirko
authors.identifier: http://orcid.org/0000-0002-4256-7174
authors.initials: M
authors.lastname: Betti
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2 / Q000737:chemistry / Q000378:metabolism*; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007422:Intestines; D000074011:Peptide Transporter 1; D027981:Symporters / Q000737:chemistry / Q000378:metabolism*
chemical_list: D051273:Glucose Transporter Type 2; D006020:Glycopeptides; D000074011:Peptide Transporter 1; C082186:SLC15A1 protein, human; C495680:SLC2A2 protein, human; D027981:Symporters; D005947:Glucose
keywords: GLUT transporters; Pro-Hyp; Pro-Hyp-CONH-GlcN; glucosamine; glycopeptide