title: Aerobic Exercise Decreases Lung Inflammation by IgE Decrement in an OVA Mice Model.
abstract: Aerobic exercise (AE) reduces lung function decline and risk of exacerbations in asthmatic patients. However, the inflammatory lung response involved in exercise during the sensitization remains unclear. Therefore, we evaluated the effects of exercise for 2 weeks in an experimental model of sensitization and single ovalbumin-challenge. Mice were divided into 4 groups: mice non-sensitized and not submitted to exercise (Sedentary, n=10); mice non-sensitized and submitted to exercise (Exercise, n=10); mice sensitized and exposed to ovalbumin (OVA, n=10); and mice sensitized, submitted to exercise and exposed to OVA (OVA+Exercise, n=10). 24 h after the OVA/saline exposure, we counted inflammatory cells from bronchoalveolar fluid (BALF), lung levels of total IgE, IL-4, IL-5, IL-10 and IL-1ra, measurements of OVA-specific IgG1 and IgE, and VEGF and NOS-2 expression via western blotting. AE reduced cell counts from BALF in the OVA group (p<0.05), total IgE, IL-4 and IL-5 lung levels and OVA-specific IgE and IgG1 titers (p<0.05). There was an increase of NOS-2 expression, IL-10 and IL-1ra lung levels in the OVA groups (p<0.05). Our results showed that AE attenuated the acute lung inflammation, suggesting immunomodulatory properties on the sensitization process in the early phases of antigen presentation in asthma.
authors.affiliation: State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil.
authors.forename: Deborah
authors.initials: D
authors.lastname: Camargo Hizume-Kunzler
authors.affiliation: Nove de Julho University - UNINOVE, Research, São Paulo, Brazil.
authors.forename: Flavia R
authors.initials: FR
authors.lastname: Greiffo
authors.affiliation: State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Fortkamp
authors.affiliation: Universidade do Estado de Santa Catarina Centro de Ciencias da Departamento de Fisioterapia, Saude e do Esporte, Florianopolis, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Ribeiro Freitas
authors.affiliation: Universidade do Estado de Santa Catarina Centro de Ciencias da Departamento de Fisioterapia, Saude e do Esporte, Florianopolis, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Keller Nascimento
authors.affiliation: School of Medicine of University of Sao Paulo, Internal Medicine, Sao Paulo, Brazil.
authors.forename: Thayse
authors.initials: T
authors.lastname: Regina Bruggemann
authors.affiliation: State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Melo Avila
authors.affiliation: Faculdade de Medicina da Universidade de São Paulo, Departamento de Clínica Médica, São Paulo, Brazil.
authors.forename: Adenir
authors.initials: A
authors.lastname: Perini
authors.affiliation: Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Franciane
authors.initials: F
authors.lastname: Bobinski
authors.affiliation: Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Morgana
authors.initials: M
authors.lastname: Duarte Silva
authors.affiliation: Universidade do Contestado Campus Concordia, Ciências Biológicas, Concordia, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Rocha Lapa
authors.affiliation: Nove de Julho University (UNINOVE), Laboratory of Pulmonary and Exercise Immunology (LABPEI) and Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), Sao Paulo, Brazil.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Paula Vieira
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Farmacologia, Florianopolis, Brazil.
authors.forename: Verônica
authors.initials: V
authors.lastname: Vargas Horewicz
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Ciências Biológicas, Florianópolis, Brazil.
authors.forename: Adair Roberto
authors.initials: AR
authors.lastname: Soares Dos Santos
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Ciências Biológicas, Florianópolis, Brazil.
authors.forename: Kelly
authors.initials: K
authors.lastname: Cattelan Bonorino
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology / Q000628:therapy; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007378:Interleukins / Q000276:immunology; D008297:Male; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010047:Ovalbumin; D010805:Physical Conditioning, Animal; D011014:Pneumonia / Q000276:immunology / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D007378:Interleukins; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D007073:Immunoglobulin E; D010047:Ovalbumin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse