title: miRNA-221 acts as an oncogenic role by directly targeting TIMP2 in non-small-cell lung carcinoma.
abstract: MicroRNA-221 (miRNA-221) plays an important role in occurrence and development of tumor. However, expression pattern and role of miRNA-221 in the non-small-cell lung carcinoma (NSCLC) remains poorly understood. In the current study, we explored the roles and the underlying mechanism of miR-221 in NSCLC by gain and loss of function analysis. We found that the expression of miRNA-221 is significantly higher in NSCLC tissues and NSCLC cells compared to normal tissues and cells, respectively (P<0.05). NSCLC cell lines SPCA1 and H1299 were transfected with miRNA-221 mimic or inhibitor, respectively, and then performed to analysis. Experiments indicated that upregulation of miRNA-221 promotes cell proliferation, cell cycle, migration, invasion in SPCA1 cells, while downregulation of miRNA-221 exhibited the opposite role. Tissue inhibitor of metallopeptidases-2 (TIMP2) mRNA or protein levels were downregulated after miRNA-221 overexpression in SPCA1 and H1299 cells, respectively. Furthermore, TIMP2 was identified as a direct target of miRNA-221. In summary, our results indicate that miRNA-221 promotes NSCLC growth and invasion through repressing the expression of TIMP2, which suggest that inhibition of miRNA-221 could be a potential target for the treatment of NSCLC.
authors.affiliation: Department of oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China. Electronic address: yinzijun@jnu.edu.cn.
authors.forename: Zijun
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D019716:Tissue Inhibitor of Metalloproteinase-2; C525709:MIR221, human; C560823:TIMP2 protein, human
keywords: H1299; MicroRNA-221; Non-small-cell lung carcinoma; SPCA1; TIMP2