title: Honey Extracted Polyphenolics Reduce Experimental Hypoxia in Human Keratinocytes Culture.
abstract: Hypoxic assault affects fundamental cellular processes and generates oxidative stress on healthy cells/molecules. Honey extracted polyphenolics (HEP) as a natural antioxidant reduced hypoxic cytotoxicity in this study. Different honey samples were physicochemically characterized to identify preferred (jamun) honey [pH 3.55 ± 0.04, conductivity (μs/cm) = 6.66 ± 0.14, water content % (w/w) = 14.70 ± 0.35, total solid content % (w/w) = 85.30 ± 0.35, phenol content (mg GAE/100 g) = 403.55 ± 0.35, flavonoid content (mg QE/100 g) = 276.76 ± 4.10, radical scavenging activity (% 500 μL) = 147.75 ± 3.13, catalase activity (absorbance at 620 nm) = 0.226 ± 0.01]. HEP was tested in different doses on hypoxic and normoxic cells (HaCaT) using viability and antioxidant assays. Cardinal molecular expressions such as cadherin-catenin-cytoskeleton complex (namely, E-cadherin, β-catenin, and F-actin), hypoxia marker (Hif 1 α), proliferation marker (Ki67), and epithelial master regulator (p63) were studied by immuno-cytochemisty (ICC) and qRT-PCR. The 0.063 mg/mL HEP demonstrated better vitality and functionality of HaCaT cells as per viability assay (*, P < 0.01) even under hypoxia. ICC and qRT-PCR observations indicated restoration of cellular survival and homeostasis under 0.063 mg/mL HEP after hypoxic assault. Furthermore, major spectral changes for nucleic acid and membrane phospholipid reorganizations by Fourier transform infrared spectroscopy illustrated a positive impact of 0.063 mg/mL HEP on hypoxic cells considering proliferation and cellular integrity. It was concluded that a specific dose of jamun HEP reduces hypoxic cytotoxicity.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur , Kharagpur 721302, West Bengal, India.
authors.forename: Amrita
authors.identifier: http://orcid.org/0000-0001-6813-9031
authors.initials: A
authors.lastname: Chaudhary
authors.affiliation: Department of Biotechnology, National Institute of Technology Sikkim , Barfung Block Ravangla Sub-Division South Sikkim, Ravangla 737139, Sikkim, India.
authors.forename: Swarnendu
authors.initials: S
authors.lastname: Bag
authors.affiliation: Banerjees' Biomedical Research Foundation , Sainthia, Birbhum 731234, West Bengal, India.
authors.forename: Provas
authors.initials: P
authors.lastname: Banerjee
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur , Kharagpur 721302, West Bengal, India.
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006722:Honey / Q000032:analysis*; D006801:Humans; D000860:Hypoxia / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000494:pharmacology*
chemical_list: D015820:Cadherins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D059808:Polyphenols
keywords: HaCaT; antioxidant; honey; hypoxia; proliferation