title: [Expression profile of miR-501-5p in lung adenocarcinoma patients from Xuanwei area].
abstract: To investigate the relationship between miR-501-5p expression and the clinicopathological factors in patients with lung adenocarcinoma in Xuanwei area.
  
  
  
  Surgical specimens of lung adenocarcinoma and paired adjacent tissues from 24 patients with lung adenocarcinoma from Xuanwei area were examined for miR-501-5p expression using microRNA microarray technique and qPCR. Chi-square test was used to analyze the association of miR-501-5P expression with the clinicopathological characteristics of the patients. Multiple regression analysis was performed to analyze the association of miR-501-5p expression with the patients' gender, age, tumor stage, and preoperative CEA level.
  
  
  
  MicroRNA microarray analysis and qPCR validation results revealed significantly upregulated expressions of miR-501-5p in patients with lung adenocarcinoma from Xuanwei area (Plt;0.01). The microarray data showed an up-regulation of miR-501-5p by 3.17 folds in lung adenocarcinoma tissue compared with the adjacent tissue (P=0.22376, FDR=0.071395). Chi-square test indicated that miR-501-5p expression level was associated with the patients' age (f=7.168, P=0.014), TNM stage (f=36.627, P<0.01), and preoperative serum CEA level (f=30.045, Plt;0.01), but not with the patients' gender (f=3.612, P=0.071). Multiple regression analysis revealed that miR-501-5p expression was positively correlated with the patients' age, TNM stage of the tumor, and serum CEA (Plt;0.05).
  
  
  
  miR-501-5p expression is up-regulated in lung adenocarcinoma with significant associations with the patients' age, TNM stages and serum CEA level in patients from Xuanwei area, suggesting its potential role in the tumorigenesis and progression of lung adenocarcinoma in Xuanwei area.
authors.affiliation: Department of Thoracic surgery, Third Affiliated Hospital of Kunming Medical University/ Tumor Hospital of Yunnan Province/ Yunnan Key Laboratory of Lung Cancer Research, Kunming 650000, China.E-mail: ady_2010@qq.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Chen
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Li
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Lei
authors.forename: Lin-Wei
authors.initials: LW
authors.lastname: Tian
authors.forename: Guang-Qiang
authors.initials: GQ
authors.lastname: Zhao
authors.forename: Qiubo
authors.initials: Q
authors.lastname: Huang
authors.forename: Yunc-Hao
authors.initials: YH
authors.lastname: Huang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D002681:China; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging
chemical_list: C582676:MIRN501 microRNA, human; D035683:MicroRNAs