title: Early Detection of Circulating Antigen and IgM-Associated Immune Complexes during Experimental Mycobacterium bovis Infection in Cattle.
abstract: The presence of circulating antigen in cattle experimentally infected withMycobacterium boviswas demonstrated using dual-path platform (DPP) technology. The antigen capture immunoassays employed rabbit polyclonal antibody recognizing predominantlyM. tuberculosiscomplex-specific epitopes and were able to detect soluble substances and whole cells of mycobacteria. The antigen found in serum appeared to be mostly bound to IgM, but not to IgG, within the immune complexes formed at early stages ofM. bovisinfection. The antigen was also detected in bile and urine, indicating possible clearance pathways. The data correlation analyses supported the idea of the role of IgM responses in antigen persistence duringM. bovisinfection. The antigen was detectable in serum months prior to detectable antibody seroconversion. This proof-of-concept study suggested the potential for improved immunodiagnostics for bovine tuberculosis.
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA klyashchenko@chembio.com.
authors.forename: Konstantin P
authors.initials: KP
authors.lastname: Lyashchenko
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA.
authors.forename: Rena
authors.initials: R
authors.lastname: Greenwald
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA.
authors.forename: Alina
authors.initials: A
authors.lastname: Sikar-Gang
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA.
authors.forename: Archana A
authors.initials: AA
authors.lastname: Sridhara
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Johnathan
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Lambotte
authors.affiliation: Chembio Diagnostic Systems, Inc., Medford, New York, USA.
authors.forename: Javan
authors.initials: J
authors.lastname: Esfandiari
authors.affiliation: National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, USA.
authors.forename: Mayara F
authors.initials: MF
authors.lastname: Maggioli
authors.affiliation: National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, USA.
authors.forename: Tyler C
authors.identifier: http://orcid.org/0000-0001-6779-7649
authors.initials: TC
authors.lastname: Thacker
authors.affiliation: National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, USA.
authors.forename: Mitchell V
authors.initials: MV
authors.lastname: Palmer
authors.affiliation: National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, USA.
authors.forename: W Ray
authors.initials: WR
authors.lastname: Waters
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood*; D000942:Antigens, Bacterial / Q000032:analysis / Q000097:blood*; D001646:Bile / Q000382:microbiology; D002417:Cattle; D007075:Immunoglobulin M / Q000097:blood*; D009163:Mycobacterium bovis / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods*; D014380:Tuberculosis, Bovine / Q000175:diagnosis*; D014556:Urine / Q000382:microbiology
chemical_list: D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D007075:Immunoglobulin M
keywords: Mycobacterium bovis; antibody; antigen; immune complexes; tuberculosis