title: Enhanced acyclovir delivery using w/o type microemulsion: preclinical assessment of antiviral activity using murine model of zosteriform cutaneous HSV-1 infection.
abstract: The present study was aimed to develop and evaluate a microemulsion-based dermal drug delivery of an antiviral agent, acyclovir. A water-in-oil microemulsion was prepared using isopropyl myristate, Tween 20, Span 20, water and dimethylsulphoxide. It was characterized for drug content, stability, globule size, pH, viscosity and ex vivo permeation through mice skin. In vivo antiviral efficacy of optimized formulation was assessed in female Balb/c mice against herpes simplex virus-I (HSV-I)-induced infection. It was observed that optimized formulation when applied 24-h post-infection could completely inhibit the development of cutaneous herpetic lesions vis-à-vis marketed cream.
authors.affiliation: a Division of Pharmaceutics, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University , Chandigarh , India.
authors.forename: Amanpreet
authors.initials: A
authors.lastname: Kaur
authors.affiliation: a Division of Pharmaceutics, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University , Chandigarh , India.
authors.forename: Gajanand
authors.initials: G
authors.lastname: Sharma
authors.affiliation: a Division of Pharmaceutics, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University , Chandigarh , India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Gupta
authors.affiliation: b Postgraduate Institute of Medical Education and Research , Chandigarh , India.
authors.forename: Radha Kanta
authors.initials: RK
authors.lastname: Ratho
authors.affiliation: a Division of Pharmaceutics, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Studies, Panjab University , Chandigarh , India.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Katare
mesh_terms: D000212:Acyclovir / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D004655:Emulsions; D005260:Female; D006561:Herpes Simplex / Q000188:drug therapy; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009821:Oils / Q000737:chemistry*; D010539:Permeability; D012867:Skin / Q000378:metabolism; D012995:Solubility; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D009821:Oils; D014867:Water; D000212:Acyclovir
keywords: Acyclovir; HSV-1; dermal; microemulsion