title: Triptolide inhibits the growth of osteosarcoma by regulating microRNA-181a via targeting PTEN gene in vivo and vitro.
abstract: We aimed to study the anti-tumor effects of triptolide on osteosarcoma and the related molecular mechanisms. The cell viability, apoptosis portion, tumor size, tumor weight, and invasion of osteosarcoma cells were determined. The relative level of microRNA-181 in osteosarcoma tissues and the adjacent tissues was determined by quantitative real-time reverse transcription polymerase chain reaction. The target gene of microRNA-181a was determined and verified by luciferase report assay. At last, osteosarcoma cells were treated with triptolide and triptolide + microRNA-181a mimics to verify the relationship between triptolide and microRNA-181a. Triptolide inhibited the cell viability, promoted the apoptosis, decreased the tumor size and weight, and reduced the invasion of osteosarcoma cells. The level of microRNA-181a in osteosarcoma cells decreased significantly after treating with triptolide, and the relative level of microRNA-181a in osteosarcoma tissues was markedly higher than that in the adjacent tissues. PTEN was reported and verified the direct target gene of microRNA-181a. The overexpression of microRNA-181a decreased the inhibition of triptolide on osteosarcoma proliferation and promotion on osteosarcoma apoptosis. In conclusion, triptolide inhibited cell growth and invasion of osteosarcoma by regulating microRNA-181a via targeting PTEN gene in vivo and vitro.
authors.affiliation: 1 Department of Pediatrics, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Jiang
authors.affiliation: 2 Department of Clinical Laboratory, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Fang
authors.affiliation: 3 Department of Ophthalmology, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.
authors.forename: Hongxu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.|5 Hangzhou Orthopedic Institute, Hangzhou, China.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.|5 Hangzhou Orthopedic Institute, Hangzhou, China.
authors.forename: Maoqiang
authors.initials: M
authors.lastname: Li
authors.affiliation: 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.|5 Hangzhou Orthopedic Institute, Hangzhou, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Jiang
authors.affiliation: 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.|5 Hangzhou Orthopedic Institute, Hangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.affiliation: 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.|5 Hangzhou Orthopedic Institute, Hangzhou, China.
authors.forename: Liulong
authors.initials: L
authors.lastname: Zhu
authors.affiliation: 4 Department of Orthopedic Surgery, Hangzhou First People's Hospital, Nanjing Medical University, Hangzhou, China.|5 Hangzhou Orthopedic Institute, Hangzhou, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Bian
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000502:physiology*; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000188:drug therapy* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D010616:Phenanthrenes / Q000494:pharmacology*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D004224:Diterpenes; D004852:Epoxy Compounds; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D010616:Phenanthrenes; C001899:triptolide; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: PTEN; Triptolide; miR-181a; osteosarcoma