title: Polyanionic Antimicrobial Membranes: An Experimental and Theoretical Study.
abstract: Polycationic polymers have been widely used as antimicrobial materials because of their broad spectrum activity and potential use as new antibiotics. Herein, we report the synthesis of polyanionic antimicrobial membranes by in situ photo-cross-linking of a sulfate based anionic monomer, followed by cation-exchange with organic (quaternary ammonium or imidazolium) or metal (Ag+, Cu2+, Fe3+, Zn2+, Na+, K+) cations. The resultant polyanionic membranes show high and broad spectrum antibacterial activities against both bacteria (Escherichia coli, Staphylococcus aureus) and fungi (Candida albicans ). In addition, the polyanionic antimicrobial membranes efficiently inhibited the formation of biofilms by SC5314 and its crk1 gene deleted (Δcrk1) C. albicans strains. Furthermore, the synthesized polyanionic membranes exhibit good blood compatibility, low cytotoxicity and long-term antibacterial stability, demonstrating safe antimicrobial materials in the application of healthcare.
authors.affiliation: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University , Suzhou, 215123, China.
authors.forename: Jiangna
authors.identifier: 0000-0002-4052-252X
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Zhongshan Hospital, Fudan University , 180 Fenglin Road, Shanghai, 200032, China.
authors.forename: Qiming
authors.identifier: 0000-0001-7762-1794
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Institute of Technical Biology & Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences , 350 Shushanhu Road, Anhui, 230031, China.
authors.forename: Rongwei
authors.identifier: 0000-0002-5279-7328
authors.initials: R
authors.lastname: Shi
authors.affiliation: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University , Suzhou, 215123, China.
authors.forename: Zhiqiang
authors.identifier: 0000-0001-7973-7985
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Zhongshan Hospital, Fudan University , 180 Fenglin Road, Shanghai, 200032, China.
authors.forename: Hailei
authors.identifier: 0000-0003-2999-7142
authors.initials: H
authors.lastname: Mao
authors.affiliation: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University , Suzhou, 215123, China.
authors.forename: Feng
authors.identifier: 0000-0001-9269-7025
authors.initials: F
authors.lastname: Yan