title: Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study.
abstract: Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 109propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties.
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Stéphanie-Marie
authors.initials: SM
authors.lastname: Deutsch
authors.affiliation: MaIAGE, UR1404, INRA, 78352 Jouy-en-Josas, France.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Mariadassou
authors.affiliation: MaIAGE, UR1404, INRA, 78352 Jouy-en-Josas, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Nicolas
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Parayre
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Rozenn
authors.initials: R
authors.lastname: Le Guellec
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Victoria
authors.initials: V
authors.lastname: Chuat
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Peton
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Le Maréchal
authors.affiliation: MaIAGE, UR1404, INRA, 78352 Jouy-en-Josas, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Burati
authors.affiliation: MaIAGE, UR1404, INRA, 78352 Jouy-en-Josas, France.
authors.forename: Valentin
authors.initials: V
authors.lastname: Loux
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Briard-Bion
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Jardin
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection and Immunity of Lille, 59000 Lille, France.
authors.forename: Coline
authors.initials: C
authors.lastname: Plé
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection and Immunity of Lille, 59000 Lille, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Foligné
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Jan
authors.affiliation: STLO, UMR 1253, INRA, Agrocampus Ouest, 35000, Rennes, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Falentin
mesh_terms: D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002611:Cheese / Q000382:microbiology*; D003593:Cytoplasm / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D023281:Genomics; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D010802:Phylogeny; D000070002:Propionibacterium freudenreichii / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
chemical_list: D000893:Anti-Inflammatory Agents; D001426:Bacterial Proteins; C508609:IL10 protein, human; D016753:Interleukin-10