title: Prenatal microarray comparative genomic hybridization: Experience from the two first years of activity at the Lyon university-hospital.
abstract: OBJECTIVE
  This study aims to describe how microarray comparative genomic hybridization (aCGH) has shifted to become a prenatal diagnosis tool at the Lyon university-hospital.
  
  
  METHODS
  This retrospective study included all patients who were referred in the 3 pluridisciplinary centers for prenatal diagnosis of the Lyon university-hospital and who received a prenatal aCGH between June 2013 and June 2015. aCGH was systematically performed in parallel with a karyotype, using the PréCytoNEM array design.
  
  
  RESULTS
  A total of 260 microarrays were performed for the following indications: 249 abnormal ultrasounds (95.8%), 7 characterizations of chromosomal rearrangements (2.7%), and 4 twins with no abnormal ultrasounds (1.5%). With a resolution of 1 mega base, we found 235 normal results (90.4%), 23 abnormal results (8.8%) and 2 non-returns (0.8%). For the chromosomal rearrangements visible on the karyotype, aCGH identified all of the 12 unbalanced rearrangements and did not identify the 2 balanced rearrangements. Among the fetuses with normal karyotypes, 11 showed abnormal microarray results, corresponding to unbalanced cryptic chromosomal rearrangements (4.2%).
  
  
  CONCLUSIONS
  Transferring aCGH to a prenatal diagnosis at the Lyon university-hospital has increased the detection rate of chromosomal abnormalities by 4.2% compared to the single karyotype.
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France; Université Claude-Bernard Lyon 1, 69008 Lyon, France. Electronic address: linda.pons@chu-lyon.fr.
authors.forename: L
authors.initials: L
authors.lastname: Pons
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: M
authors.initials: M
authors.lastname: Till
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: E
authors.initials: E
authors.lastname: Alix
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: C
authors.initials: C
authors.lastname: Abel
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: D
authors.initials: D
authors.lastname: Boggio
authors.affiliation: Département d'obstétrique et de gynécologie, groupement hospitalier Est, HCL, 69500 Bron, France.
authors.forename: A
authors.initials: A
authors.lastname: Bordes
authors.affiliation: Département d'obstétrique et de gynécologie, centre hospitalier de la Croix-Rousse, HCL, 69004 Lyon, France.
authors.forename: J
authors.initials: J
authors.lastname: Caloone
authors.affiliation: Département d'obstétrique et de gynécologie, centre hospitalier Lyon Sud, HCL, 69310 Pierre-Bénite, France.
authors.forename: F C
authors.initials: FC
authors.lastname: Raskin
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France; Université Claude-Bernard Lyon 1, 69008 Lyon, France; Équipe Gendev, CNRS UMR 5292, Inserm U1028, centre de recherche en neuroscience de Lyon, 69500 Bron, France.
authors.forename: N
authors.initials: N
authors.lastname: Chatron
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: M-P
authors.initials: MP
authors.lastname: Cordier
authors.affiliation: Département d'obstétrique et de gynécologie, centre hospitalier de la Croix-Rousse, HCL, 69004 Lyon, France.
authors.forename: A
authors.initials: A
authors.lastname: Fichez
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: A
authors.initials: A
authors.lastname: Labalme
authors.affiliation: Département d'obstétrique et de gynécologie, groupement hospitalier Est, HCL, 69500 Bron, France.
authors.forename: C
authors.initials: C
authors.lastname: Lajeunesse
authors.affiliation: Département d'obstétrique et de gynécologie, centre hospitalier Lyon Sud, HCL, 69310 Pierre-Bénite, France.
authors.forename: É
authors.initials: É
authors.lastname: Liaras
authors.affiliation: Département d'obstétrique et de gynécologie, groupement hospitalier Est, HCL, 69500 Bron, France.
authors.forename: M
authors.initials: M
authors.lastname: Massoud
authors.affiliation: Département d'obstétrique et de gynécologie, groupement hospitalier Est, HCL, 69500 Bron, France.
authors.forename: J
authors.initials: J
authors.lastname: Miribel
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: E
authors.initials: E
authors.lastname: Ollagnon
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France; Université Claude-Bernard Lyon 1, 69008 Lyon, France; Équipe Gendev, CNRS UMR 5292, Inserm U1028, centre de recherche en neuroscience de Lyon, 69500 Bron, France.
authors.forename: C
authors.initials: C
authors.lastname: Schluth-Bolard
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France.
authors.forename: A
authors.initials: A
authors.lastname: Vichier-Cerf
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France; Université Claude-Bernard Lyon 1, 69008 Lyon, France; Équipe Gendev, CNRS UMR 5292, Inserm U1028, centre de recherche en neuroscience de Lyon, 69500 Bron, France.
authors.forename: P
authors.initials: P
authors.lastname: Edery
authors.affiliation: Université Claude-Bernard Lyon 1, 69008 Lyon, France; Département d'obstétrique et de gynécologie, centre hospitalier Lyon Sud, HCL, 69310 Pierre-Bénite, France.
authors.forename: J
authors.initials: J
authors.lastname: Attia
authors.affiliation: Département d'obstétrique et de gynécologie, centre hospitalier de la Croix-Rousse, HCL, 69004 Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Huissoud
authors.affiliation: Université Claude-Bernard Lyon 1, 69008 Lyon, France; Département d'obstétrique et de gynécologie, centre hospitalier de la Croix-Rousse, HCL, 69004 Lyon, France.
authors.forename: R C
authors.initials: RC
authors.lastname: Rudigoz
authors.affiliation: Département d'obstétrique et de gynécologie, groupement hospitalier Est, HCL, 69500 Bron, France.
authors.forename: J
authors.initials: J
authors.lastname: Massardier
authors.affiliation: Université Claude-Bernard Lyon 1, 69008 Lyon, France; Département d'obstétrique et de gynécologie, groupement hospitalier Est, HCL, 69500 Bron, France.
authors.forename: P
authors.initials: P
authors.lastname: Gaucherand
authors.affiliation: Service de génétique, groupement hospitalier Est, HCL, 59, boulevard Pinel, 69677 Bron, France; Université Claude-Bernard Lyon 1, 69008 Lyon, France; Équipe Gendev, CNRS UMR 5292, Inserm U1028, centre de recherche en neuroscience de Lyon, 69500 Bron, France.
authors.forename: D
authors.initials: D
authors.lastname: Sanlaville
mesh_terms: D000293:Adolescent; D000328:Adult; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis*; D055028:Comparative Genomic Hybridization; D005260:Female; D005602:France; D006785:Hospitals, University; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012189:Retrospective Studies; D055815:Young Adult
keywords: Abnormal ultrasounds; Characterization of chromosomal rearrangements; Microarray comparative genomic hybridization; Prenatal diagnosis