title: A rotating cerium anode X-ray system allows visualization of intramural coronary vessels after cardiac stem cell therapy for myocardial infarction.
abstract: Conventional angiography is insufficient for evaluating the therapeutic effect of cardiac regeneration therapy. A microangiographic X-ray system using a cerium anode was developed. Cerium has a characteristic X-ray with a peak at 34.6 keV, which allows visualization of tiny amounts of iodine. The performance of the cerium anode X-ray system was evaluated in two excised normal canine hearts and in excised ischemic canine hearts treated with c-kit-positive cardiac stem cells (5 canines) or without cells (5 control canines). In the normal canines, branches penetrating from the left anterior descending artery into the myocardium were visualized, down to third-order branches. In just the treated hearts treated with stem cells, small vessels characterized by irregular vessel walls were observed. The cerium anode X-ray system allowed visualization of microvessels in excised ischemic canine hearts, and may evaluate the effect of cardiac stem cell therapy.
authors.affiliation: Department of Cardiovascular Surgery, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan. chitty@is.icc.u-tokai.ac.jp.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tanaka
authors.affiliation: Tokai University Institute of Innovative Science and Technology, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Hosoda
authors.affiliation: Department of Physiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Yoshimori
authors.initials: Y
authors.lastname: Ikeya
authors.affiliation: Support Center for Medical Research and Education, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Shinozaki
authors.affiliation: Department of Physiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Kikue
authors.initials: K
authors.lastname: Todoroki
authors.affiliation: Department of Physiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Shizuma
authors.affiliation: NHK Engineering Services, Inc., Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiraishi
authors.affiliation: Department of Physiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Fukuyama
authors.affiliation: Department of Cardiovascular Surgery, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ueda
authors.affiliation: Department of Physiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan. coronary@is.icc.u-tokai.ac.jp.
authors.forename: Hidezo
authors.initials: H
authors.lastname: Mori
mesh_terms: D000818:Animals; D002563:Cerium; D003331:Coronary Vessels / Q000000981:diagnostic imaging*; D004195:Disease Models, Animal; D004285:Dogs; D006321:Heart / Q000000981:diagnostic imaging*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000628:therapy; D033581:Stem Cell Transplantation / Q000379:methods*; D014965:X-Rays
chemical_list: D002563:Cerium
keywords: C-kit-positive cardiac stem cells; Canine models; Cerium anode; Microangiography; Myocardial infarction