title: Transplantation of PDGF-AA-Overexpressing Oligodendrocyte Precursor Cells Promotes Recovery in Rat Following Spinal Cord Injury.
abstract: Our previous study showed that Schwann cells (SCs) promote survival, proliferation and migration of co-transplanted oligodendrocyte progenitor cells (OPCs) and neurological recovery in rats with spinal cord injury (SCI). A subsequentin vitrostudy confirmed that SCs modulated OPC proliferation and migration by secreting platelet-derived growth factor (PDGF)-AA and fibroblast growth factor-2 (FGF)-2. We also found that PDGF-AA stimulated OPC proliferation and their differentiation into oligodendrocytes (OLs) at later stages. We therefore speculated that PDGF-AA administration can exert the same effect as SC co-transplantation in SCI repair. To test this hypothesis, in this study we investigated the effect of transplanting PDGF-AA-overexpressing OPCs in a rat model of SCI. We found that PDGF-AA overexpression in OPCs promoted their survival, proliferation, and migration and differentiation into OLsin vivo. OPCs overexpressing PDGF-AA were also associated with increased myelination and tissue repair after SCI, leading to the recovery of neurological function. These results indicate that PDGF-AA-overexpressing OPCs may be an effective treatment for SCI.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeBengbu, China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical CollegeBengbu, China.
authors.forename: Zong-Feng
authors.initials: ZF
authors.lastname: Yao
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeBengbu, China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical CollegeBengbu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeBengbu, China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical CollegeBengbu, China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeBengbu, China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical CollegeBengbu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College Bengbu, China.
authors.forename: An-You
authors.initials: AY
authors.lastname: Zhu
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College Bengbu, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College Bengbu, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College Bengbu, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xi
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College Bengbu, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College Bengbu, China.
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeBengbu, China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical CollegeBengbu, China.
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeBengbu, China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical CollegeBengbu, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
keywords: functional recovery; oligodendrocyte progenitor cells; platelet-derived growth factor-AA; spinal cord injury; transplantation