title: Protective Effect of Cordycepin on Experimental Testicular Ischemia/Reperfusion Injury in Rats.
abstract: OBJECTIVE
  To investigate the role of cordycepin in testicular ischemia/reperfusion injury in rats.
  
  
  METHODS
  Forty Wistar albino rats were randomly divided into four groups, as follows: group one, control (C); group two, torsion and ischemia (I); group three: detorsion with ischemia-reperfusion (IR); and group four, detorsion/cordycepin. The rats were then analyzed macromorphologically and histopathologically, and blood tests were performed. Specifically, the total oxidant status (TOS) and total antioxidant status (TAS) were determined, and malondialdehyde (MDA), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β levels were analyzed. In addition, pyknotic nuclei, spermatozoa, edema, and hemorrhage were assessed.
  
  
  RESULTS
  When the IR and cordycepin groups were compared with the other groups, there was a statistically significant decrease in TNF-α and MDA levels (p < 0.05). Increased TAS levels were observed in the cordycepin group compared with the control group. TOS levels were significantly increased in the I and IR groups, but decreased in the cordycepin group (p < 0.05). Similar effects were observed in tissue biochemistry analysis. Histopathological evaluations revealed that the spermatozoa count was decreased in the I and IR groups. However, there was an increase in the cordycepin group, as well as a statistically significant difference between the IR and cordycepin groups (p < 0.01). Finally, edema and inflammation were increased in the I and IR groups, but decreased in the cordycepin group.
  
  
  CONCLUSIONS
  Histological and biochemical findings revealed that cordycepin protected against IR-induced testicular injury.
authors.affiliation: a Department of Pediatric Surgery , Medical Faculty of Dicle University , Diyarbakir , Turkey.
authors.forename: Mehmet Hanifi
authors.initials: MH
authors.lastname: Okur
authors.affiliation: a Department of Pediatric Surgery , Medical Faculty of Dicle University , Diyarbakir , Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Arslan
authors.affiliation: a Department of Pediatric Surgery , Medical Faculty of Dicle University , Diyarbakir , Turkey.
authors.forename: Bahattin
authors.initials: B
authors.lastname: Aydogdu
authors.affiliation: a Department of Pediatric Surgery , Medical Faculty of Dicle University , Diyarbakir , Turkey.
authors.forename: Hikmet
authors.initials: H
authors.lastname: Zeytun
authors.affiliation: a Department of Pediatric Surgery , Medical Faculty of Dicle University , Diyarbakir , Turkey.
authors.forename: Erol
authors.initials: E
authors.lastname: Basuguy
authors.affiliation: a Department of Pediatric Surgery , Medical Faculty of Dicle University , Diyarbakir , Turkey.
authors.forename: Mehmet Serif
authors.initials: MS
authors.lastname: Arslan
authors.affiliation: b Department of Pathology , Dicle University Medical School Diyarbakir , Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Ibiloglu
authors.affiliation: c Department of Biochemistry , Dicle University Medical School Diyarbakir , Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D003839:Deoxyadenosines / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D003839:Deoxyadenosines; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D015850:Interleukin-6; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; C058120:cordycepin
keywords: Testis; cordycepin; cytokines; ischemia/reperfusion; rat; torsion