title: Mutation in sorghumLOW GERMINATION STIMULANT 1alters strigolactones and causesStrigaresistance.
abstract: Strigais a major biotic constraint to sorghum production in semiarid tropical Africa and Asia. Genetic resistance to this parasitic weed is the most economically feasible control measure. Mutant alleles at theLGS1(LOW GERMINATION STIMULANT 1) locus drastically reduceStrigagermination stimulant activity. We provide evidence that the responsible gene atLGS1codes for an enzyme annotated as a sulfotransferase and show that functional loss of this gene results in a change of the dominant strigolactone (SL) in root exudates from 5-deoxystrigol, a highly activeStrigagermination stimulant, to orobanchol, an SL with opposite stereochemistry. Orobanchol, although not previously reported in sorghum, functions in the multiple SL roles required for normal growth and environmental responsiveness but does not stimulate germination ofStrigaThis work describes the identification of a gene regulatingStrigaresistance and the underlying protective chemistry resulting from mutation.
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN 47907.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gobena
authors.affiliation: Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands.
authors.forename: Mahdere
authors.initials: M
authors.lastname: Shimels
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN 47907.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Rich
authors.affiliation: Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands.
authors.forename: Carolien
authors.initials: C
authors.lastname: Ruyter-Spira
authors.affiliation: Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands.
authors.forename: Harro
authors.initials: H
authors.lastname: Bouwmeester
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN 47907.
authors.forename: Satish
authors.initials: S
authors.lastname: Kanuganti
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
authors.forename: Tesfaye
authors.initials: T
authors.lastname: Mengiste
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN 47907; gejeta@purdue.edu.
authors.forename: Gebisa
authors.initials: G
authors.lastname: Ejeta
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D006790:Host-Parasite Interactions; D007783:Lactones / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D009154:Mutation; D053147:Plant Exudates / Q000737:chemistry; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D045868:Sorghum / Q000235:genetics* / Q000469:parasitology*; D034362:Striga / Q000502:physiology*
chemical_list: D007783:Lactones; D053147:Plant Exudates; D010940:Plant Proteins
keywords: Striga; gene; sorghum; stereochemistry; strigolactone