title: Downregulation of sorting nexin 10 is associated with overexpression of miR-30d during liver cancer progression in rats.
abstract: As of 2012, liver cancer was the second leading cause of death worldwide, and hepatocellular carcinoma is the most common primary cancer of the liver. The identification of molecules that might be molecular markers or therapeutic targets is urgently needed to improve clinical management. Based on a microarray analysis performed in our laboratory, we selected six genes-namely, ANXA2, DYNLT1, PFKP, PLA2G7, KRT19, and SNX10-as candidates for validation as tumor markers of liver cancer in a rat model. Their patterns of overexpression in preneoplastic lesions and established tumors at 10 different time points between 24 h and 18 months were analyzed to identify putative tumor markers for further studies. We validated the microarray results by quantitative reverse transcription polymerase chain reaction, which revealed high transcriptional expression for five of the genes, consistent with their high protein expression during cancer progression reported in the literature. However, studies of the association of sorting nexin 10 with different types of cancer are limited, prompting further study. The characterization of sorting nexin 10 in preneoplastic lesions and established tumors revealed messenger RNA overexpression and a simultaneous decrease in sorting nexin 10 protein expression. A group of microRNAs related to sorting nexin 10 messenger RNA were selected based on a data analysis conducted using miRDB and microrna.org . An analysis of the expression of these microRNAs revealed an increase in the transcription of microRNA-30d whenever the sorting nexin 10 protein was downregulated. These results suggest that sorting nexin 10 is a potential liver cancer marker exhibiting characteristics of a putative suppressor protein that is likely regulated by microRNA-30d.
authors.affiliation: 1 Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: Nancy
authors.initials: N
authors.lastname: Cervantes-Anaya
authors.affiliation: 2 Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ponciano-Gómez
authors.affiliation: 1 Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: Guadalupe Soledad
authors.initials: GS
authors.lastname: López-Álvarez
authors.affiliation: 1 Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.|3 Unidad Académica de Ciencias Químico Biológicas y Farmacéutica, Universidad Autónoma de Nayarit, Tepic, México.
authors.forename: Christian
authors.initials: C
authors.lastname: Gonzalez-Reyes
authors.affiliation: 1 Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: Sergio
authors.initials: S
authors.lastname: Hernández-Garcia
authors.affiliation: 1 Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: María Asunción
authors.initials: MA
authors.lastname: Cabañas-Cortes
authors.affiliation: 2 Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: José Efraín
authors.initials: JE
authors.lastname: Garrido-Guerrero
authors.affiliation: 1 Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ciudad de México, México.
authors.forename: Saúl
authors.initials: S
authors.lastname: Villa-Treviño
mesh_terms: D000818:Animals; D000071187:Autophagy-Related Protein 5 / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011916:Rats, Inbred F344; D058305:Sorting Nexins / Q000032:analysis / Q000235:genetics* / Q000502:physiology
chemical_list: C545865:Atg5 protein, rat; D000071187:Autophagy-Related Protein 5; D014408:Biomarkers, Tumor; C556157:MIRN30 microRNA, rat; D035683:MicroRNAs; D058305:Sorting Nexins
keywords: Sorting nexin 10; hepatocellular carcinoma; microRNA; tumor suppressor