title: PI3K Overexpression and PIK3CA Mutations Are Associated with Age, Tumor Staging, and Other Clinical Characteristics in Chinese Patients with Esophageal Squamous Cell Carcinoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the association of PI3K expression and PIK3CA mutations with various clinical features in Chinese patients diagnosed with esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  The study included 112 patients diagnosed with ESCC from Jan 2013 to Dec 2015. Immunohistochemistry was used to determine the expression of PI3K. PIK3CA mutations were determined by sequencing. Statistical analysis was done using SPSS 19.0 software.
  
  
  RESULTS
  PI3K protein was expressed in 81.3% (91/112) of all ESCC samples, whereas it was found in only 4.9% (5/56) of adjacent normal cells. This rate of expression of PI3K was significantly higher in ESCC tissues (p < 0.001). PI3K protein expression was highly correlated with age, lymph node metastasis, and clinical stage (p < 0.05), but not with gender, location, tobacco use, alcohol use, or degree of differentiation. PIK3CA gene mutations were highly correlated with age, tobacco use, and clinical stage (p < 0.05), but not with gender, location, alcohol use, lymph node metastasis, or degree of differentiation. PI3K protein expression was not statistically correlated with PIK3CA gene mutations.
  
  
  CONCLUSIONS
  PI3K overexpression and PIK3CA mutations are associated with age, tumor staging, and other clinical characteristics in Chinese patients with ESCC and thus can be further exploited as biomarkers and therapeutic targets in esophageal cancer.
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital, Fujian Medical University , Fuzhou, China .
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Lin
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital, Fujian Medical University , Fuzhou, China .
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital, Fujian Medical University , Fuzhou, China .
authors.forename: Ming-Lian
authors.initials: ML
authors.lastname: Qiu
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital, Fujian Medical University , Fuzhou, China .
authors.forename: Rong-Gang
authors.initials: RG
authors.lastname: Luo
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital, Fujian Medical University , Fuzhou, China .
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Lin
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital, Fujian Medical University , Fuzhou, China .
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000503:physiopathology; D058534:Class I Phosphatidylinositol 3-Kinases; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000503:physiopathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015870:Gene Expression; D006239:Haplotypes; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human
keywords: PI3K; PIK3CA; biomarkers; esophageal squamous cell carcinoma