title: Improvement of isolated caprine islet survival and functionality in vitro by enhancing of PDX1 gene expression.
abstract: Dead islets replaced with viable islets are a promising offer to restore normal insulin production to a person with diabetes. The main reason for establishing a new islet source for transplantation is the insufficiency of human donor pancreas while using xenogeneic islets perhaps assists this problem. The expression of PDX1 is essential for the pancreas expansion. In mature β-cells, PDX1 has several critical roles such as glucose sensing, insulin synthesis, and insulin secretion. In this study, we aimed to evaluate the expression of pancreatic duodenal homeobox-1 (PDX1) in treated caprine islets in culture and to assess the protective effects of antioxidant factors on the PDX1 gene in cultured caprine islets.
  
  
  
  Purified islets were treated with serum-free, serum, IBMX, tocopherol, or IBMX and tocopherol media. Quantitative polymerase chain reaction and Western blotting were carried out to compare the expression levels of PDX1 in treated purified islets cultured with different media.
  
  
  
  Islets treated with IBMX/tocopherol exhibited the highest fold change in the relative expression of PDX1 on day 5 post-treatment (relative expression: 6.80±2.08), whereas serum-treated islets showed the lowest fold changes in PDX1 expression on day 5 post-treatment (0.67±0.36), as compared with the expression on day 1 post-treatment. Insulin production and viability tests of purified islets showed superiority of islet at supplemented serum-free media with IBMX/tocopherol compared to other cultures (53.875%±1.59%).
  
  
  
  Our results indicated that supplemented serum-free medium with tocopherol and IBMX enhances viability and PDX1 gene expression compared to serum-added and serum-free media.
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Homayoun
authors.identifier: 0000-0002-4590-8704
authors.initials: H
authors.lastname: Hani
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Zeenathul Nazariah
authors.initials: ZN
authors.lastname: Allaudin
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Mohd-Azmi
authors.initials: MA
authors.lastname: Mohd-Lila
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Kazhal
authors.initials: K
authors.lastname: Sarsaifi
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Mina
authors.initials: M
authors.lastname: Rasouli
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Yew Joon
authors.initials: YJ
authors.lastname: Tam
authors.affiliation: Department of Veterinary Preclinical, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Tengku-Azmi
authors.initials: TA
authors.lastname: Tengku-Ibrahim
authors.affiliation: Department of Animal Reproduction, Agro-Biotechnology Institute Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Abas Mazni
authors.initials: AM
authors.lastname: Othman
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D003470:Culture Media; D016895:Culture Media, Serum-Free; D015870:Gene Expression / Q000187:drug effects; D005801:Genes, Homeobox; D006041:Goats / Q000235:genetics* / Q000502:physiology*; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology*; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D024505:Tocopherols / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D016895:Culture Media, Serum-Free; D018398:Homeodomain Proteins; D007328:Insulin; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein; D024505:Tocopherols; D015056:1-Methyl-3-isobutylxanthine
keywords: PDX1 gene expression; antioxidant supplement; caprine islet; islet survival