title: Polydiacetylene-Coated Sensor Strip for Immunochromatic Detection of Xylella fastidiosa subsp. fastidiosa.
abstract: This study presents a sensor strip for user-friendly, naked-eye detection of Xylella fasitdiosa, the bacterial causal agent of Pierce's disease in grapevine. This sensor uses anti- X. fastidiosa antibodies conjugated to a polydiacetylene layer on a polyvinylidene fluoride strip to generate specific color transitions and discriminate levels of the pathogen. The detection limit of the sensor is 0.8 × 108cells/mL, which is similar to bacterial load in grapevine 18 days following bacterial inoculation. This sensor enables equipment-free detection that is highly desirable for in-field diagnostic tools in resource-limited settings.
authors.affiliation: 1 Department of Mechanical Engineering, University of California, Riverside, CA, USA.|2 Department of Bioengineering, University of California, Riverside, CA, USA.
authors.forename: Jessica T
authors.initials: JT
authors.lastname: Wen
authors.affiliation: 3 Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA.
authors.forename: Polrit
authors.initials: P
authors.lastname: Viravathana
authors.affiliation: 3 Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Ingel
authors.affiliation: 3 Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA.
authors.forename: Caroline
authors.initials: C
authors.lastname: Roper
authors.affiliation: 1 Department of Mechanical Engineering, University of California, Riverside, CA, USA.|2 Department of Bioengineering, University of California, Riverside, CA, USA.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tsutsui
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D012680:Sensitivity and Specificity; D027843:Vitis; D044167:Xylella / Q000302:isolation & purification*
keywords: Sensor strip; Xylella fastidiosa; immunochromatic; polydiacetylene