title: Bedside Immune Monitoring: An Automated Immunoassay Platform for Quantification of Blood Biomarkers in Patient Serum within 20 Minutes.
abstract: This Article presents an automated, compact, and self-contained system for sensitive quantitative detection of blood biomarkers. A disposable microfluidic chip, prefilled with biomarker-specific reagents and magnetic beads, can be processed fully automatically by a readout platform, enabling an immunoassay-based analysis with a processing time from sample incubation to signal analysis of 20 min. Novel concepts for on-chip vortexing of the magnetic beads and on-chip reagent storage and actuation were developed. A lens-free photodiode readout system represents a cost-efficient approach for detecting the chemiluminescent signal. IL-8 spiked serum samples were measured with a high reproducibility and a limit of detection of 2.05 pg·mL-1. The system was validated with undiluted serum samples collected from trauma patients at the intensive care unit. The developed platform demonstrated good correlation with the clinical reference method, and the clinical trajectory course of IL-8 could be sufficiently followed. With an automated assay approach and an easily adaptable protocol, this portable platform has the potential to be utilized as a universal instrument for analyzing proteins in small sample volumes (<25 μL) in point-of-care settings.
authors.affiliation: Molecular Diagnostics, Health & Environment Department, AIT Austrian Institute of Technology GmbH , 1190 Vienna, Austria.|Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, A-1200 Vienna, Austria.
authors.forename: Helene
authors.identifier: 0000-0001-7772-4099
authors.initials: H
authors.lastname: Zirath
authors.affiliation: Biegler GmbH , Allhangstrasse 18a, 3001 Mauerbach, Austria.
authors.forename: Guntram
authors.initials: G
authors.lastname: Schnetz
authors.affiliation: Molecular Diagnostics, Health & Environment Department, AIT Austrian Institute of Technology GmbH , 1190 Vienna, Austria.|Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, A-1200 Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Glatz
authors.affiliation: Surgical Research Laboratories and Core Facility Flow Cytometry, Medical University of Vienna , 1090 Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Spittler
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, A-1200 Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.affiliation: Molecular Diagnostics, Health & Environment Department, AIT Austrian Institute of Technology GmbH , 1190 Vienna, Austria.
authors.forename: Johannes R
authors.initials: JR
authors.lastname: Peham
mesh_terms: D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D016209:Interleukin-8 / Q000097:blood* / Q000276:immunology; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D015166:Monitoring, Immunologic / Q000379:methods*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D015415:Biomarkers; C508606:CXCL8 protein, human; D016209:Interleukin-8