title: Human Gyrovirus-Apoptin Interferes with the Cell Cycle and Induces G2/M Arrest Prior to Apoptosis.
abstract: The human gyrovirus-Apoptin (HGyv-Apoptin) is a protein that gained attention because it is selectively cytotoxic toward cancer cells. In this study, we have investigated the effect of HGyv-Apoptin on cell cycle progression of cancer cells. We also compared HGyv-Apoptin's action to its homologue chicken anemia virus Apoptin (CAV-Apoptin). We show that HGyv-Apoptin induces G2/M arrest in cancer cells. This is at least in part due to the fact that HGyv-Apoptin induces an abnormal spindle formation in mitotic cells that do not progress properly throughout the cell cycle. HGyv-Apoptin most likely inhibits APC function leading to a sustained cyclin-B1-expression. These results indicate that HGyv-Apoptin has a similar mechanism of action as its homolog CAV-Apoptin and further supports its cancer therapeutic potential.
authors.affiliation: Department of Biology, Faculty of Sciences, Tunis University, 2092, Tunis, Tunisia. wiemchaabane27@hotmail.fr.
authors.forename: Wiem
authors.initials: W
authors.lastname: Chaabane
authors.affiliation: Department of Human Anatomy and Cell Science, Faculty of Health Sciences, College of Medicine, University of Manitoba, Winnipeg, MB, Canada.|Health Policy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghavami
authors.affiliation: Faculty of Physiotherapy, The Jerzy Kukuczka Academy of Physical Education in Katowice, Katowice, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Małecki
authors.affiliation: ENT Department, School of Medicine, Medical University of Silesia in Katowice, Katowice, Poland. bioappl@gmail.com.
authors.forename: Marek J
authors.identifier: http://orcid.org/0000-0001-9518-1411
authors.initials: MJ
authors.lastname: Łos
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D017727:Chicken anemia virus / Q000276:immunology*; D018173:Circoviridae Infections / Q000378:metabolism*; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism; D059565:G2 Phase Cell Cycle Checkpoints; D046810:Gyrovirus / Q000276:immunology*; D045325:HCT116 Cells; D006801:Humans
chemical_list: D036022:Capsid Proteins; D056744:Cyclin B1; C091717:VP3 protein, Chicken anemia virus; D064173:Anaphase-Promoting Complex-Cyclosome
keywords: APC; Apoptin; Cell cycle; Cyclin B1; PKTCs