title: Generation and characterization of induced pluripotent stem cells from guinea pig fetal fibroblasts.
abstract: Induced pluripotent stem cells (iPS) represent an important tool to develop disease‑modeling assays, drug testing assays and cell‑based replacement therapies. The application of iPS in these fields requires the development of suitable animal models. Of the suitable species, guinea pigs are particularly important and offer significant advantages. Successful iPS generation has been accomplished in a number of species; however, it has not been reported in the guinea pig. The present study successfully generated iPS from guinea pigs (giPS) using single polycistronic virus transduction with mouse octamer‑binding transcription factor 4 (Oct4), sex determining region Y‑box 2 (Sox2), Kruppel‑like factor 4 and c‑Myc. The giPS cell lines were cultured in media containing leukemia inhibitory factor and guinea pig fibroblast cells were used as feeder cells. These cultures were expanded under feeder‑free culture conditions using ESGRO Complete Plus Clonal Grade medium containing 15% fetal bovine serum on gelatin‑coated dishes. The resultant cells had a normal karyotype, exhibited alkaline phosphatase activity and expressed the pluripotency markers Oct4, Sox2 and Nanog. The cells differentiated in vivo to form teratomas that contained all three germ layers of the tissue cells. The generation of giPS may facilitate future studies investigating the mechanisms underlying innate immunity, particularly for tuberculosis. These experiments provide proof of principle that iPS technology may be adapted to use the guinea pig as a model of human diseases.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Zhejiang Sci‑Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Zhejiang Sci‑Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Ouyang
authors.initials: O
authors.lastname: Li
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Department of Biochemistry and Molecular Biology, College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, P.R. China.
authors.forename: Chengwen
authors.initials: C
authors.lastname: He
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Department of Biochemistry and Molecular Biology, College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Department of Biochemistry and Molecular Biology, College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Department of Biochemistry and Molecular Biology, College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, P.R. China.
authors.forename: Xiaoming Liu
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Department of Biochemistry and Molecular Biology, College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, P.R. China.
authors.forename: Yujiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Zhejiang Sci‑Tech University, Hangzhou, Zhejiang 310018, P.R. China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D065150:Cellular Reprogramming / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006168:Guinea Pigs; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D007621:Karyotyping; D051379:Mice; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D014157:Transcription Factors