title: Coxiella burnetii isolates originating from infected cattle induce a more pronounced proinflammatory cytokine response compared to isolates from infected goats and sheep.
abstract: Coxiella burnetii is the causative agent of Q fever. Although the prevalence of C. burnetii in cattle is much higher than in goats and sheep, infected cattle are rarely associated with human outbreaks. We investigated whether the immune response of humans differs after contact with C. burnetii isolates from different host origins or with different multilocus variable number of tandem repeat analysis (MLVA) genotypes. Cytokine responses were measured in human peripheral blood mononuclear cells (PBMCs) stimulated with 16 C. burnetii isolates with known MLVA genotype from goats, sheep, cattle, acute and chronic Q fever patients. Coxiella burnetii isolates originating from cattle induce significantly more IL-1β, TNF-α and IL-22 than the isolates from goats, sheep or chronic Q fever patients. Comparing the cytokine induction of the isolates based on their MVLA genotype did not reveal differences in response between the MLVA genotypes. The proinflammatory cytokine response induced in human PBMCs by C. burnetii isolates from cattle may explain the low incidence of human Q fever outbreaks caused by cattle. The cytokine profile of PBMCs stimulated with C. burnetii isolates from chronic Q fever patients resembles isolates from goats. Furthermore, cytokine responses seem to be depending on host origin than on MLVA genotype.
authors.affiliation: Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.|Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands.
authors.forename: Anne
authors.initials: A
authors.lastname: Ammerdorffer
authors.affiliation: Department of Infection Biology, Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands.
authors.forename: Runa
authors.initials: R
authors.lastname: Kuley
authors.affiliation: Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands.
authors.forename: Annemieke
authors.initials: A
authors.lastname: Dinkla
authors.affiliation: Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.
authors.forename: Leo A B
authors.initials: LAB
authors.lastname: Joosten
authors.affiliation: Laboratory for Diagnosis and Prevention of Rickettsial and Chlamydial Infections, Institute of Virology, Slovak Academy of Sciences Bratislava, 811 04 Staré Mesto, Slovakia.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Toman
authors.affiliation: Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands.
authors.forename: Hendrik-Jan
authors.initials: HJ
authors.lastname: Roest
authors.affiliation: Department of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.|Department of Internal Medicine, Canisius-Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands.|Department of Medical Microbiology and Infectious Diseases, Canisius- Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands.
authors.forename: Tom
authors.initials: T
authors.lastname: Sprong
authors.affiliation: Department of Animal health and Welfare, Livestock Research Wageningen UR, 6708 WD Wageningen, The Netherlands.
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Rebel
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016997:Coxiella burnetii / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016207:Cytokines / Q000378:metabolism*; D005838:Genotype; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018598:Minisatellite Repeats; D011778:Q Fever / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D016207:Cytokines
keywords: Coxiella burnetii; Q fever; cattle; cytokines; immune response; strain diversity