title: Histological changes, lipid metabolism and oxidative stress in the liver of Bufo gargarizans exposed to cadmium concentrations.
abstract: Chinese toad (Bufo gargarizans) were exposed to different concentrations of cadmium (5, 50, 100, 200 and 500 μg Cd L-1) from Gosner stage 3-42. Metamorphosis rate, body weight, total length and body length were measured. Histological alterations in thyroid gland and liver were examined. Changes in hepatocyte were also examined using Transmission electron microscopic. In addition, the mRNA expression of several genes involved in lipid metabolism, oxidative stress and thyroid hormones signaling pathways were also measured. Our results showed that 200 and 500 μg Cd L-1decreased the metamorphosis rate and inhibited the body size of B. gargarizans larvae at G42. Moreover, histological examinations have clearly exhibited that cadmium caused liver damage. Ultrastructural examination revealed lipid accumulation and abnormal mitochondria. Exposure to 200 and 500 μg Cd L-1significantly up-regulated mRNA expression of D2, SOD, GPx, ACC and FAE, but down-regulated mRNA expression of TRα, TRβ, PPARα, ACOX, CPT and SCP. However, low Cd concentration (5, 50 and 100) exposure did not cause any effect in genes expression. Thus, we conclude that high Cd concentrations could affect the normal processes of lipid metabolism though increasing lipid synthesis and reducing the ability of fatty acid β-oxidation, and disturb thyroid hormone pathways in liver, and induced oxidative stress. In addition, lipid metabolism might be regulated by THs. To our knowledge, the present study is the first to report the influence of cadmium on hepatic lipid metabolism in B. gargarizans and will greatly provide new insights into cadmium hepatotoxicity in amphibian.
authors.affiliation: College of Life Science, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Life Science, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Engineering, Chang'an University, Xi'an 710054, China; Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Xi'an 710062, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chai
authors.affiliation: College of Life Science, Shaanxi Normal University, Xi'an 710119, China. Electronic address: hongyuanwang@snnu.edu.cn.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002025:Bufonidae / Q000033:anatomy & histology / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D004781:Environmental Exposure; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008675:Metamorphosis, Biological / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D013961:Thyroid Gland / Q000187:drug effects; D013963:Thyroid Hormones / Q000378:metabolism
chemical_list: D013963:Thyroid Hormones; D002104:Cadmium
keywords: Bufo gargarizans; Cadmium; Lipid metabolism; Liver; Oxidative stress