title: Enzymatic synthesis of N-succinyl chitosan-collagen peptide copolymer and its characterization.
abstract: Collagen peptide (COP) grafted N-succinyl chitosan (NSC) was prepared by using microbial transglutaminase (MTGase) as biocatalyst. The catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The degree of substitution (DS) of N-succinyl chitosan-collagen peptide (NSC-COP)depended on the reaction time, the reaction temperature, the mass ratio of COP to NSC and the mass ratio of MTGase to NSC. NSC-COP showed excellent moisture absorption and retention properties. Antioxidant activities of varying DS and concentration of NSC-COP were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl. Methylthiazol tetrazolium (MTT) assay exhibited that at a suitable concentration NSC-COP with different DS value could promote L929 mouse fibroblasts effectively. The animal experiment indicated that the wound covered with NSC-COP were completely filled with new epithelium within 2 weeks without any significant adverse side reactions. Therefore, the results may contribute to finding the application of NSC-COP in pharmaceutical and biomedical fields.
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Ge
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Shengqiong
authors.initials: S
authors.lastname: Zou
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Huigao
authors.initials: H
authors.lastname: Wen
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lihongfan2000@hotmail.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Anatomy, College of Medicine, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002056:Burns / Q000628:therapy; D048271:Chitosan / Q000138:chemical synthesis*; D003094:Collagen / Q000138:chemical synthesis*; D016166:Free Radical Scavengers; D010455:Peptides / Q000138:chemical synthesis*; D051381:Rats; D013696:Temperature; D014945:Wound Healing
chemical_list: D016166:Free Radical Scavengers; D010455:Peptides; D003094:Collagen; D048271:Chitosan
keywords: Antioxidant activity; Cell viability; Microbial transglutaminase; N-Succinyl chitosan-collagen peptide; Wound healing