title: Purification of Fla2 Flagella of Rhodobacter sphaeroides.
abstract: The photosynthetic bacterium R. sphaeroides expresses two flagellar systems that are encoded by two complete gene clusters that have distinct phylogenetic origins. The isolation and purification of the Filament-Hook Basal Body (F-HBB) or the Hook Basal Body (HBB) structure is a troublesome task given the complexity of this nano-machine that is composed of multiple loosely bound substructures that can be lost during the isolation and purification procedure. A successful procedure requires adjustments to the standard method established for Salmonella. In this chapter, we describe a detailed protocol to isolate and purify the Fla2 F-HBB and HBB from R. sphaeroides a photosynthetic bacterium that has a complex intracellular membrane system that frequently interferes with isolation of high-quality samples.
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Cicuito Exterior s/n, Mexico City, 04510, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: de la Mora
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Camarena
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Cicuito Exterior s/n, Mexico City, 04510, Mexico. gdreyfus@ifc.unam.mx.
authors.forename: Georges
authors.initials: G
authors.lastname: Dreyfus
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D064652:Basal Bodies / Q000378:metabolism; D005407:Flagella / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology; D012242:Rhodobacter sphaeroides / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins
keywords: Bacterial flagella; HBB purification; Motility organelle; Rhodobacter sphaeroides