title: Characterizing the lipid and metabolite changes associated with placental function and pregnancy complications using ion mobility spectrometry-mass spectrometry and mass spectrometry imaging.
abstract: Successful pregnancy is dependent upon discrete biological events, which include embryo implantation, decidualization, and placentation. Problems associated with each of these events can cause infertility or conditions such as preeclampsia. A greater understanding of the molecular changes associated with these complex processes is necessary to aid in identifying treatments for each condition. Previous nuclear magnetic resonance spectroscopy and mass spectrometry studies have been used to identify metabolites and lipids associated with pregnancy-related complications. However, due to limitations associated with conventional implementations of both techniques, novel technology developments are needed to more fully understand the initiation and development of pregnancy related problems at the molecular level. In this perspective, we describe current analytical techniques for metabolomic and lipidomic characterization of pregnancy complications and discuss the potential for new technologies such as ion mobility spectrometry-mass spectrometry and mass spectrometry imaging to contribute to a better understanding of the molecular changes that affect the placenta and pregnancy outcomes.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Burnum-Johnson
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. Electronic address: erin.baker@pnnl.gov.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. Electronic address: thomas.metz@pnnl.gov.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D010064:Embryo Implantation; D005260:Female; D006801:Humans; D000075663:Ion Mobility Spectrometry; D050356:Lipid Metabolism; D055432:Metabolomics / Q000379:methods* / Q000639:trends; D008954:Models, Biological; D010920:Placenta / Q000378:metabolism* / Q000502:physiology / Q000503:physiopathology; D010929:Placentation; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism* / Q000503:physiopathology; D021241:Spectrometry, Mass, Electrospray Ionization; D018629:Spectrometry, Mass, Secondary Ion
keywords: Deep placentation; Ion mobility spectrometry-mass spectrometry; Lipidome; Mass spectrometry imaging; Metabolome; Nano-DESI; Pregnancy complications