title: Alteration of high-density lipoprotein functionality in Alzheimer's disease patients.
abstract: The aims of the present study were to determine whether high-density lipoprotein (HDL) functionality-mediated cholesterol efflux is altered in Alzheimer's disease and to investigate the role and effect of amyloid-beta (Aβ) in the regulation of the anti-atherogenic activity of HDL. Eighty-seven elderly subjects were recruited, of whom 27 were healthy, 27 had mild cognitive impairment (MCI), and 33 had mild Alzheimer's disease (mAD). Our results showed that total cholesterol levels are negatively correlated with the Mini-Mental State Examination (MMSE) score (r = -0.2602, p = 0.0182). HDL from the mAD patients was less efficient at mediating cholesterol efflux from J774 macrophages (p < 0.05) than HDL from the healthy subjects and MCI patients. While HDL from the MCI patients was also less efficient at mediating cholesterol efflux than HDL from the healthy subjects, the difference was not significant. Interestingly, the difference between the healthy subjects and the MCI and mAD patients with respect to the capacity of HDL to mediate cholesterol efflux disappeared when ATP-binding cassette transporter A1 (ABCA1)-enriched J774 macrophages were used. HDL fluidity was significantly inversely correlated with the MMSE scores (r = -0.4137, p < 0.009). In vitro measurements of cholesterol efflux using J774 macrophages showed that neither Aβ1-40nor Aβ1-42stimulate cholesterol efflux from unenriched J774 macrophages in basal or ABCA1-enriched J774 macrophages.
authors.affiliation: a Department of Medicine, Geriatrics Service, Faculty of Medicine and Biological Sciences, University of Sherbrooke, Sherbrooke, QC J1H 4N4, Canada.
authors.forename: Paméla
authors.initials: P
authors.lastname: Camponova
authors.affiliation: a Department of Medicine, Geriatrics Service, Faculty of Medicine and Biological Sciences, University of Sherbrooke, Sherbrooke, QC J1H 4N4, Canada.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Le Page
authors.affiliation: a Department of Medicine, Geriatrics Service, Faculty of Medicine and Biological Sciences, University of Sherbrooke, Sherbrooke, QC J1H 4N4, Canada.|b Department of Biology, University Sultan Moulay Slimane, Beni Mellal, Morocco.
authors.forename: Hicham
authors.initials: H
authors.lastname: Berrougui
authors.affiliation: a Department of Medicine, Geriatrics Service, Faculty of Medicine and Biological Sciences, University of Sherbrooke, Sherbrooke, QC J1H 4N4, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Lamoureux
authors.affiliation: c Department of Internal Medicine II, Center for Medical Research, University of Tübingen, Tübingen, Germany.
authors.forename: Graham
authors.initials: G
authors.lastname: Pawelec
authors.affiliation: d Department of Pathophysiology, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: M Jacek
authors.initials: MJ
authors.lastname: Witkowski
authors.affiliation: a Department of Medicine, Geriatrics Service, Faculty of Medicine and Biological Sciences, University of Sherbrooke, Sherbrooke, QC J1H 4N4, Canada.
authors.forename: Tamas
authors.initials: T
authors.lastname: Fulop
authors.affiliation: a Department of Medicine, Geriatrics Service, Faculty of Medicine and Biological Sciences, University of Sherbrooke, Sherbrooke, QC J1H 4N4, Canada.
authors.forename: Abdelouahed
authors.initials: A
authors.lastname: Khalil
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood* / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000097:blood; D001692:Biological Transport; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D010446:Peptide Fragments / Q000097:blood
chemical_list: D016229:Amyloid beta-Peptides; D008075:Lipoproteins, HDL; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D002784:Cholesterol
keywords: Alzheimer’s disease; HDL; amyloid beta; bêta-amyloïde; cholesterol efflux; efflux de cholestérol; maladie d’Alzheimer