title: Design of Poly-l-Glutamate-Based Complexes for pDNA Delivery.
abstract: Due to the polyanionic nature of DNA, typically cationic or neutral delivery vehicles have been used for gene delivery. As a new approach, this study focuses on the design, development, and validation of nonviral polypeptide-based carriers for oligonucleotide delivery based on a negatively charged poly-l-glutamic acid (PGA) backbone partly derivatized with oligoaminoamide residues. To this end, PGA-derivatives modified with different pentameric succinyl tetraethylene pentamines (Stp5) are designed. Optionally, histidines for modulation of endosomal buffer capacity and cysteines for pDNA complex stabilization are included, followed by characterization of biophysical properties and gene transfer efficiency in N2a neuroblastoma or 4T1 breast cancer cells.
authors.affiliation: Polymer Therapeutics Lab, Centro de Investigación Príncipe Felipe (CIPF), C/Eduardo Primo Yúfera 3, Valencia, 46012, Spain.
authors.forename: Amaya
authors.initials: A
authors.lastname: Niño-Pariente
authors.affiliation: Polymer Therapeutics Lab, Centro de Investigación Príncipe Felipe (CIPF), C/Eduardo Primo Yúfera 3, Valencia, 46012, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Armiñán
authors.affiliation: Pharmaceutical Biotechnology, Department of Pharmacy, Center for Nanoscience, Ludwig-Maximilians-Universität, Butenandtstr. 5-13, Building D, 81377, Munich, Germany.
authors.forename: Sören
authors.initials: S
authors.lastname: Reinhard
authors.affiliation: Pharmaceutical Biotechnology, Department of Pharmacy, Center for Nanoscience, Ludwig-Maximilians-Universität, Butenandtstr. 5-13, Building D, 81377, Munich, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Scholz
authors.forename: Petra
authors.initials: P
authors.lastname: Kos
authors.affiliation: Pharmaceutical Biotechnology, Department of Pharmacy, Center for Nanoscience, Ludwig-Maximilians-Universität, Butenandtstr. 5-13, Building D, 81377, Munich, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Wagner
authors.affiliation: Polymer Therapeutics Lab, Centro de Investigación Príncipe Felipe (CIPF), C/Eduardo Primo Yúfera 3, Valencia, 46012, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Vicent
mesh_terms: D000577:Amides / Q000737:chemistry*; D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005029:Ethylenediamines / Q000737:chemistry*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011099:Polyglutamic Acid / Q000737:chemistry* / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry
chemical_list: D000577:Amides; D005029:Ethylenediamines; D013388:Succinimides; D049452:Green Fluorescent Proteins; D011099:Polyglutamic Acid; D006639:Histidine; D004247:DNA; D008156:Luciferases; D003545:Cysteine
keywords: pDNA delivery; polyglutamates; polymer therapeutics; polyplex