title: Integrated mRNA and lncRNA expression profiling for exploring metastatic biomarkers of human intrahepatic cholangiocarcinoma.
abstract: Long noncoding RNAs (lncRNAs) is crucial for various human cancers, but the function and mechanism of lncRNAs is largely unknown in human intrahepatic cholangiocarcinoma (ICC), the second most common liver cancer. In this study, we performed transcriptomic profiling of ICC and normal tissues, and found 2148 lncRNAs and 474 mRNAs were significantly upregulated, whereas 568 lncRNAs and 409 mRNAs were downregulated in ICC tissues. Enrichment analysis suggests these differentially expressed genes mainly focus on response to stimulus, development, and cell proliferation. Further, potential lncRNAs involved in five signaling pathways (ERBB, JAK/STAT, MAPK, VEGF and WNT) were constructed by highly co-expressed with mRNAs in these signaling pathways. The differentially expressed lncRNA-mRNA co-regulated signaling pathways in ICC were further confirmed by lncRNA target prediction. Finally, the differentially expressed lncRNAs were confirmed by quantitative real-time PCR in 32 paired ICC and adjacent tissues. The correlation analysis between the expression levels of lncRNAs and clinicopathologic characteristics showed that EMP1-008, ATF3-008, and RCOR3-013 were observed significantly downregulated in ICC with tumor metastasis. These findings suggested that lncRNA expression profiling in ICC is profoundly different from that in noncancerous tissues, and lncRNA may be used as a potential diagnostic and prognostic biomarker for ICC metastasis.
authors.affiliation: Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong UniversityShanghai, China; Department of General Surgery, Shengli Oilfield Central Hospital, Binzhou Medical UniversityBinzhou, Shandong, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Miaoyan
authors.initials: M
authors.lastname: Wei
authors.affiliation: Department of General Surgery, The Second Hospital, Xiamen University Xiamen, China.
authors.forename: Peiyi
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of General Surgery, Qingpu Hospital, Fudan University Shanghai Medical College Shanghai, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Dalian Medical University, Dalian Medical University Dalian, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gong
authors.affiliation: Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Quan
authors.affiliation: Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Tang
keywords: biomarkers; intrahepatic cholangiocarcinoma; lncRNA; metastasis; microarray