title: From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species.
abstract: Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000, Liège, Belgium. j.ceusters@ulg.ac.be.
authors.forename: J
authors.initials: J
authors.lastname: Ceusters
authors.affiliation: Mont Le Soie Equine Research Center, Mont Le Soie, 1 6690, Vielsalm, Belgium.
authors.forename: J-Ph
authors.initials: JP
authors.lastname: Lejeune
authors.affiliation: Department of Clinical Sciences, Equine Surgery, Faculty of Veterinary Medicine B41, University of Liège, Sart Tilman, 4000, Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Sandersen
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000, Liège, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Niesten
authors.affiliation: Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Université Libre de Bruxelles, Campus Erasme, Brussels, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Lagneaux
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000, Liège, Belgium.|Mont Le Soie Equine Research Center, Mont Le Soie, 1 6690, Vielsalm, Belgium.|Department of Clinical Sciences, Equine Surgery, Faculty of Veterinary Medicine B41, University of Liège, Sart Tilman, 4000, Liège, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Serteyn
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004285:Dogs; D006736:Horses; D006801:Humans; D056747:Immunomodulation; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013552:Swine