title: Generation and Application of Monoclonal Antibody Against Lycopene.
abstract: A monoclonal antibody (Mab) against lycopene was developed from hybridoma clones obtained from BALB/c mice immunized with trans-isomer of lycopene (t-lycopene, t-LC) conjugated with colloidal gold particles. An alternating immunization schedule which included injection of both formulations of immunogen (without and with Freund's adjuvant) was most effective in the elucidation of a measurable immune response to the t-Lycopene conjugate. Selected hybridoma clones were able to produce an Mab positive in competition assay. In particular, preincubation of 6B9 Mabs with t-LC abolished the ability of 6B9 Mabs to bind LC in the competition assay. Mabs produced by other clones (4F10, 4A3, and 3B12) worked similarly. Analysis of antigen specificity showed that 6B9 Mab raised against t-LC did not recognize other carotenoids such as lutein and carotene. Mab 6B9 was shown to recognize lycopene on a glass surface and in the settings of indirect immunofluorescence experiments performed in cultured hepatocytes and alveolar macrophages incubated with and without lycopene, as well as in sebum and corneocyte specimens from the skin of volunteers supplemented with nutraceutical formulation of lycopene. Newly generated Mabs against lycopene may provide a valuable tool for different analytical assays of lycopene content in various biological, agricultural, and food products.
authors.affiliation: 1 Gamaleya Research Center of Epidemiology and Microbiology , Ministry of Health, Moscow, Russia .
authors.forename: Valeriy V
authors.initials: VV
authors.lastname: Tsibezov
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Yuriy K
authors.initials: YK
authors.lastname: Bashmakov
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Pristenskiy
authors.affiliation: 1 Gamaleya Research Center of Epidemiology and Microbiology , Ministry of Health, Moscow, Russia .
authors.forename: Naylia A
authors.initials: NA
authors.lastname: Zigangirova
authors.affiliation: 1 Gamaleya Research Center of Epidemiology and Microbiology , Ministry of Health, Moscow, Russia .
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Kostina
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Natalya E
authors.initials: NE
authors.lastname: Chalyk
authors.affiliation: 1 Gamaleya Research Center of Epidemiology and Microbiology , Ministry of Health, Moscow, Russia .
authors.forename: Alexey Y
authors.initials: AY
authors.lastname: Kozlov
authors.affiliation: 1 Gamaleya Research Center of Epidemiology and Microbiology , Ministry of Health, Moscow, Russia .
authors.forename: Elena Y
authors.initials: EY
authors.lastname: Morgunova
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Marina P
authors.initials: MP
authors.lastname: Chernyshova
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Marina V
authors.initials: MV
authors.lastname: Lozbiakova
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Nigel H
authors.initials: NH
authors.lastname: Kyle
authors.affiliation: 2 Lycotec Ltd , Cambridge, United Kingdom .
authors.forename: Ivan M
authors.initials: IM
authors.lastname: Petyaev
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D000918:Antibody Specificity; D000941:Antigens / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002338:Carotenoids / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D005620:Freund's Adjuvant / Q000008:administration & dosage; D017979:Gold Colloid / Q000008:administration & dosage / Q000737:chemistry; D022781:Hepatocytes / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007117:Immunization, Secondary / Q000379:methods*; D018796:Immunoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D014975:Lutein; D000077276:Lycopene; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D017979:Gold Colloid; D018796:Immunoconjugates; D002338:Carotenoids; D005620:Freund's Adjuvant; D000077276:Lycopene; D014975:Lutein
keywords: antibody; hybridoma; lycopene