title: The Effects of Sertraline on Fathead Minnow (Pimephales promelas) Growth and Steroidogenesis.
abstract: The purpose of this study was to evaluate the steroidogenic effects of sertraline, a popular selective serotonin reuptake inhibitor, on larval fathead minnow (FHM; Pimephales promelas) and adult FHM. Larvae were exposed to 0.1, 1, and 10 µg/L sertraline for 28 days and analyzed for differential mRNA expression of 11β-hydroxysteroid dehydrogenase (11β-HSD), 20β-hydroxysteroid dehydrogenase (20β-HSD), aromatase (CYP19a), nuclear thyroid receptor alpha (TRα), and normalized to RP-L8. Adult FHM were exposed to 3 or 10 µg/L sertraline for 7 days and analyzed for differential expression of the same genes with the addition of thyroid receptor beta (TRβ). Larval FHM exposed to 0.1 μg/L had a significant upregulation of both 20β-HSD and TRα while adult FHM exposed to 10 µg/L had a significant upregulation of 11β-HSD expression in brain tissue. The significance of these findings with respect to survival, growth and reproduction are currently unknown, but represent areas for future research.
authors.affiliation: Department of Biology, Institute of Applied Sciences, University of North Texas, 1704 West Mulberry Room 215, Denton, TX, 76201, USA. dcarty@go.olemiss.edu.
authors.forename: D R
authors.identifier: http://orcid.org/0000-0002-6406-7058
authors.initials: DR
authors.lastname: Carty
authors.affiliation: Department of Biology, Institute of Applied Sciences, University of North Texas, 1704 West Mulberry Room 215, Denton, TX, 76201, USA.
authors.forename: D
authors.initials: D
authors.lastname: Hala
authors.affiliation: Department of Biology, Institute of Applied Sciences, University of North Texas, 1704 West Mulberry Room 215, Denton, TX, 76201, USA.
authors.forename: D B
authors.initials: DB
authors.lastname: Huggett
mesh_terms: D000818:Animals; D001141:Aromatase; D003530:Cyprinidae / Q000378:metabolism / Q000502:physiology*; D005260:Female; D008297:Male; D012098:Reproduction / Q000187:drug effects; D020280:Sertraline / Q000633:toxicity*; D013256:Steroids / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D013256:Steroids; D014874:Water Pollutants, Chemical; D001141:Aromatase; C561410:CYP19A1 protein, human; D020280:Sertraline
keywords: Endocrine disruption; Fathead minnow; Pharmaceuticals