title: Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress.
abstract: The present study was aimed at investigating the effect of amifostine on myocardial ischemia/reperfusion (I/R) injury of mice and H9c2 cells cultured with TBHP (tert-butyl hydroperoxide). The results showed that pretreatment with amifostine significantly attenuated cell apoptosis and death, accompanied by decreased reactive oxygen species (ROS) production and lower mitochondrial potential (ΔΨm). In vivo, amifostine pretreatment alleviated I/R injury and decreased myocardial apoptosis and infarct area, which was paralleled by increased superoxide dismutase (SOD) and reduced malondialdehyde (MDA) in myocardial tissues, increased Bcl2 expression, decreased Bax expression, lower cleaved caspase-3 level, fewer TUNEL positive cells, and fewer DHE-positive cells in heart. Our results indicate that amifostine pretreatment has a protective effect against myocardial I/R injury via scavenging ROS.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Shao-Ze
authors.initials: SZ
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lu-Yuan
authors.initials: LY
authors.lastname: Tao
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jiao-Ni
authors.initials: JN
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Xu
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Yang-Jing
authors.initials: YJ
authors.lastname: Xue
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Kai-Yu
authors.initials: KY
authors.lastname: Huang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jia-Feng
authors.initials: JF
authors.lastname: Lin
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Kang-Ting
authors.identifier: 0000-0003-3891-8741
authors.initials: KT
authors.lastname: Ji
mesh_terms: D004999:Amifostine / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D051379:Mice; D008928:Mitochondria / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000517:prevention & control; D018384:Oxidative Stress / Q000187:drug effects*; D065129:Pre-Exposure Prophylaxis; D011837:Radiation-Protective Agents / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D011837:Radiation-Protective Agents; D004999:Amifostine