title: AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging.
abstract: Near-IR fluorescence (NIRF) imaging is a new technology using IR fluorescent protein (iRFP) gene labelling and is potentially useful forin vivoapplications. In the present study, we expressed iRFP and the TNF-related apoptosis inducing ligand gene in mesenchymal stem cells (MSCs) using adeno-associated virus (AAV) and showed that iRFP-labelled MSCs can be detected by fluorescence microscopy. We injected mice with MSCs labelled with AAV-iRFP, which we were then able to detect by whole-animal NIRF imaging. Our technique provides a visualizable, convenient and sensitive platform for research on tracking the fate of transplanted MSC cellsin vivo.
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops,School of Life Science, Shanghai University, Shanghai 200436, PR China.|KAS key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Lan
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops,School of Life Science, Shanghai University, Shanghai 200436, PR China.|KAS key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.affiliation: KAS key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, PR China kdswgc2011@163.com Liuxm@sibet.ac.cn chenqincc@staff.shu.edu.cn.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: KAS key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, PR China kdswgc2011@163.com Liuxm@sibet.ac.cn chenqincc@staff.shu.edu.cn.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops,School of Life Science, Shanghai University, Shanghai 200436, PR China kdswgc2011@163.com Liuxm@sibet.ac.cn chenqincc@staff.shu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D058948:Cell Tracking / Q000379:methods*; D000229:Dependovirus / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007259:Infrared Rays; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D014162:Transfection
chemical_list: D008164:Luminescent Proteins
keywords: Adeno-associated virus; MSC; Site-specific integration; iRFP; in vivo imaging