title: Virulence of Pseudomonas syringae pv. tomato DC3000 Is Influenced by the Catabolite Repression Control Protein Crc.
abstract: Pseudomonas syringae infects diverse plant species and is widely used as a model system in the study of effector function and the molecular basis of plant diseases. Although the relationship between bacterial metabolism, nutrient acquisition, and virulence has attracted increasing attention in bacterial pathology, it is largely unexplored in P. syringae. The Crc (catabolite repression control) protein is a putative RNA-binding protein that regulates carbon metabolism as well as a number of other factors in the pseudomonads. Here, we show that deletion of crc increased bacterial swarming motility and biofilm formation. The crc mutant showed reduced growth and symptoms in Arabidopsis and tomato when compared with the wild-type strain. We have evidence that the crc mutant shows delayed hypersensitive response (HR) when infiltrated into Nicotiana benthamiana and tobacco. Interestingly, the crc mutant was more susceptible to hydrogen peroxide, suggesting that, in planta, the mutant may be sensitive to reactive oxygen species generated during pathogen-associated molecular pattern-triggered immunity (PTI). Indeed, HR was further delayed when PTI-induced tissues were challenged with the crc mutant. The crc mutant did not elicit an altered PTI response in plants compared with the wild-type strain. We conclude that Crc plays an important role in growth and survival during infection.
authors.affiliation: 1 School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.; and.
authors.forename: Suma
authors.initials: S
authors.lastname: Chakravarthy
authors.affiliation: 1 School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.; and.
authors.forename: Bronwyn G
authors.initials: BG
authors.lastname: Butcher
authors.affiliation: 1 School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.; and.
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 1 School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.; and.|2 Emerging Pests & Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, U.S.A.
authors.forename: Katherine
authors.initials: K
authors.lastname: D'Amico
authors.affiliation: 1 School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.; and.
authors.forename: Matthew
authors.initials: M
authors.lastname: Coster
authors.affiliation: 1 School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.; and.|2 Emerging Pests & Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, NY 14853, U.S.A.
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Filiatrault
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D057465:Catabolite Repression / Q000187:drug effects; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000633:toxicity; D018551:Solanum lycopersicum / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D009068:Movement; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D057865:Plant Immunity / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D044224:Pseudomonas syringae / Q000472:pathogenicity* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D014774:Virulence / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; C101896:crc protein, Pseudomonas; D006861:Hydrogen Peroxide