title: Mitophagy in Refractory Temporal Lobe Epilepsy Patients with Hippocampal Sclerosis.
abstract: This study aimed to determine if there is an association between mitophagy and refractory temporal lobe epilepsy (rTLE) with hippocampal sclerosis. During epilepsy surgery, we collected tissue samples from the hippocampi and temporal lobe cortexes of rTLE patients with hippocampal sclerosis (as diagnosed by a pathologist). Transmission electron microscopy (TEM) was used to study the ultrastructural features of the tissue. To probe for mitophagy, we used fluorescent immunolabeling to determine if mitochondrial and autophagosomal markers colocalized. Fourteen samples were examined. TEM results showed that early autophagosomes were present and mitochondria were impaired to different degrees in hippocampi. Immunofluorescent labeling showed colocalization of the autophagosome marker LC3B with the mitochondrial marker TOMM20 in hippocampi and temporal lobe cortexes, indicating the presence of mitophagy. Mitochondrial and autophagosomal marker colocalization was lower in hippocampus than in temporal lobe cortex (P < 0.001). Accumulation of autophagosomes and mitophagy activation are implicated in rTLE with hippocampal sclerosis. Aberrant accumulation of damaged mitochondria, especially in the hippocampus, can be attributed to defects in mitophagy, which may participate in epileptogenesis.
authors.affiliation: Neurology Department, West China Hospital, Sichuan University, 37th Guoxuexiang Road, Chengdu, 610041, China.
authors.forename: Mengqian
authors.initials: M
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital and College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Neurology Department, West China Hospital, Sichuan University, 37th Guoxuexiang Road, Chengdu, 610041, China.
authors.forename: Xiaosa
authors.initials: X
authors.lastname: Chi
authors.affiliation: Neurology Department, West China Hospital, Sichuan University, 37th Guoxuexiang Road, Chengdu, 610041, China.
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Neurology Department, West China Hospital, Sichuan University, 37th Guoxuexiang Road, Chengdu, 610041, China.
authors.forename: Weixi
authors.initials: W
authors.lastname: Xiong
authors.affiliation: West China College of Medicine, Sichuan University, Chengdu, China.
authors.forename: Siew Mun Vance
authors.initials: SMV
authors.lastname: Chiang
authors.affiliation: Neurology Department, West China Hospital, Sichuan University, 37th Guoxuexiang Road, Chengdu, 610041, China. zhoudong66@yahoo.de.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Neurology Department, West China Hospital, Sichuan University, 37th Guoxuexiang Road, Chengdu, 610041, China. jinmeili-neuro@qq.com.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D001343:Autophagy; D002648:Child; D004833:Epilepsy, Temporal Lobe / Q000000981:diagnostic imaging* / Q000503:physiopathology; D005260:Female; D006624:Hippocampus / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D008297:Male; D063306:Mitophagy; D012598:Sclerosis; D013702:Temporal Lobe / Q000473:pathology / Q000503:physiopathology; D055815:Young Adult
keywords: Autophagy; Hippocampal sclerosis; LC3B; Mitochondria; Mitophagy; Refractory temporal lobe epilepsy