title: Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases inArabidopsis.
abstract: Flowering time control integrates endogenous as well as environmental signals to promote flower development. The pathways and molecular networks involved are complex and integrate many modes of signal transduction. In plants ubiquitin mediated protein degradation pathway has been proposed to be as important mode of signaling as phosphorylation and transcription. To systematically study the role of ubiquitin signaling in the molecular regulation of flowering we have taken a genomic approach to identify flower related Ubiquitin Proteasome System components. As a large and versatile gene family the RING type ubiquitin E3 ligases were chosen as targets of the genomic screen. The complete list ofArabidopsisRING E3 ligases were retrieved and verified in theArabidopsisgenome v11 and their differential expression was used for their categorization into flower organs or developmental stages. Known regulators of flowering time or floral organ development were identified in these categories through literature search and representative mutants for each category were purchased for functional characterization by growth and morphological phenotyping. To this end, a workflow was developed for high throughput phenotypic screening of growth, morphology and flowering of nearly a thousandArabidopsisplants in one experimental round.
authors.affiliation: Department of Agricultural Sciences, University of HelsinkiHelsinki, Finland; Viikki Plant Science Centre, University of HelsinkiHelsinki, Finland.
authors.forename: Mirko
authors.initials: M
authors.lastname: Pavicic
authors.affiliation: Department of Agricultural Sciences, University of HelsinkiHelsinki, Finland; Viikki Plant Science Centre, University of HelsinkiHelsinki, Finland.
authors.forename: Katriina
authors.initials: K
authors.lastname: Mouhu
authors.affiliation: Department of Agricultural Sciences, University of HelsinkiHelsinki, Finland; Viikki Plant Science Centre, University of HelsinkiHelsinki, Finland.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Agricultural Sciences, University of HelsinkiHelsinki, Finland; Viikki Plant Science Centre, University of HelsinkiHelsinki, Finland.
authors.forename: Marcelina
authors.initials: M
authors.lastname: Bilicka
authors.affiliation: Department of Agricultural Sciences, University of Helsinki Helsinki, Finland.
authors.forename: Erik
authors.initials: E
authors.lastname: Chovanček
authors.affiliation: Department of Agricultural Sciences, University of HelsinkiHelsinki, Finland; Viikki Plant Science Centre, University of HelsinkiHelsinki, Finland.
authors.forename: Kristiina
authors.initials: K
authors.lastname: Himanen
keywords: Arabidopsis; RING E3 ligase; flower; high throughput; image based phenotyping; phenomics data analysis; ubiquitin