title: Cetylpyridinium chloride functionalized silica-coated magnetite microspheres for the solid-phase extraction and pre-concentration of ochratoxin A from environmental water samples with high-performance liquid chromatographic analysis.
abstract: A new method based on cetylpyridinium chloride coated ferroferric oxide/silica magnetic microspheres as an efficient solid-phase adsorbent was developed for the extraction and enrichment of ochratoxin A. The determination of ochratoxin A was obtained by high-performance liquid chromatography with fluorescence detection. In the presence of cetylpyridinium chloride, the adsorption capacity of ferroferric oxide/silica microspheres was 5.95 mg/g for ochratoxin A. The experimental parameters were optimized, including the amounts of ferroferric oxide/silica microspheres (20 mg) and cetylpyridinium chloride (0.18 mL, 0.5 mg/mL), pH value of media (9), ultrasonic time (5 min), elution solvent and volume [2(1 + 1) mL (washed twice, 1 mL each time) 1% acetic acid acetonitrile]. Under optimal experiment conditions, ochratoxin A had good linearity in the range of 2.5-250.0 ng/L in water samples with correlation coefficient of the calibration curve 0.9995. The limit of detection for ochratoxin A was 0.83 ng/L, and the recoveries were 89.8-96.8% with the relative standard deviation of 1.5-3.5% in environmental water samples. Furthermore, ferroferric oxide/silica microspheres show excellent reusability during extraction procedures for no less than six times.
authors.affiliation: College of Chemistry, Nanchang University, Nanchang, China.|Physical and Chemical Department, Nanchang Centre for Disease Control and Prevention, Nanchang, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Hu
authors.affiliation: College of Chemistry, Nanchang University, Nanchang, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Physical and Chemical Department, Nanchang Centre for Disease Control and Prevention, Nanchang, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Suo
authors.affiliation: College of Chemistry, Nanchang University, Nanchang, China.
authors.forename: Fangying
authors.identifier: http://orcid.org/0000-0002-8524-7904
authors.initials: F
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D002594:Cetylpyridinium; D002851:Chromatography, High Pressure Liquid; D052203:Ferrosoferric Oxide; D008863:Microspheres; D009793:Ochratoxins / Q000302:isolation & purification*; D012822:Silicon Dioxide; D052616:Solid Phase Extraction; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D009793:Ochratoxins; D014874:Water Pollutants, Chemical; C025589:ochratoxin A; D012822:Silicon Dioxide; D002594:Cetylpyridinium; D052203:Ferrosoferric Oxide
keywords: cetylpyridinium chloride; environmental water samples; magnetic microspheres; ochratoxin A