title: Upregulation of microRNA 142-3p in the peripheral blood and urinary cells of kidney transplant recipients with post-transplant graft dysfunction.
abstract: We analyzed microRNA (miR)-142-3p expression in leucocytes of the peripheral blood and urinary sediment cell samples obtained from kidney transplant recipients who developed graft dysfunction. Forty-one kidney transplant recipients with kidney graft dysfunction and 8 stable patients were included in the study. The groups were divided according to histological analysis into acute rejection group (n=23), acute tubular necrosis group (n=18) and stable patients group used as a control for gene expression (n=8). Percutaneous biopsies were performed and peripheral blood samples and urine samples were obtained. miR-142-3p was analyzed by real-time polymerase chain reaction. The group of patients with acute tubular necrosis presented significantly higher expressions in peripheral blood (P<0.05) and urine (P<0.001) compared to the stable patients group. Also, in the peripheral blood, miR-142-3p expression was significantly higher in the acute tubular necrosis group compared to the acute rejection group (P<0.05). Urine samples of the acute rejection group presented higher expression compared to the stable patients group (P<0.001) but the difference between acute tubular necrosis and acute rejection groups was not significant in the urinary analyzes (P=0.079). miR-142-3p expression has a distinct pattern of expression in the setting of post-operative acute tubular necrosis after kidney transplantation and may potentially be used as a non-invasive biomarker for renal graft dysfunction.
authors.affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: T D
authors.initials: TD
authors.lastname: Domenico
authors.affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: G
authors.initials: G
authors.lastname: Joelsons
authors.affiliation: Unidade de Transplante Renal, Serviço de Nefrologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brasil.
authors.forename: R M
authors.initials: RM
authors.lastname: Montenegro
authors.affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.|Unidade de Transplante Renal, Serviço de Nefrologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brasil.
authors.forename: R C
authors.initials: RC
authors.lastname: Manfro
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000652:urine; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000097:blood / Q000473:pathology* / Q000652:urine; D006801:Humans; D061705:Image-Guided Biopsy; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects*; D007683:Kidney Tubular Necrosis, Acute / Q000097:blood / Q000473:pathology* / Q000652:urine; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000652:urine*; D008875:Middle Aged; D055031:Primary Graft Dysfunction / Q000097:blood / Q000473:pathology / Q000652:urine; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D066027:Transplant Recipients; D016896:Treatment Outcome; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D015415:Biomarkers; C555159:MIRN142 microRNA, human; D035683:MicroRNAs