title: LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.
abstract: We aim to determine the lncRNA targets of ΔNp63α in cervical cancer and molecular programs in cancerous differentiation. Different profiles of the lncRNAs were assayed and validated in overexpressing p63 SiHa cells (SiHa/ΔNp63α) and the control cell lines (SiHa/pCon). ENST00000422259, ENST00000447565 (Lnc-LIF-AS) and ENST00000469965, together with their related antisense mRNA DPYD (dihydropyrimidine dehydrogenase, a pyrimidine catabolic pathway gene), LIF (leukemia inhibitor factor) and FLNC (filamin C) were all notably differentially expressed in both ΔNp63α overexpression cells and knockdown cells. Here, we illustrated that ΔNp63α can inhibit the levels of LIF mRNA by direct transcription regulation and decrease LIF mRNA stability by suppressing the expression of Lnc-LIF-AS. An inverse interaction of LIF and ΔNp63α expression was as well validated in clinical samples of cervical cancer, and high level of LIF in cervical cancers was related with poor patient survival. The decrease of ΔNp63α also attenuated the differentiation of cervical cancerous cells. Suggesting that ΔNp63α may be form a complex network in regulation cervical cancerous differentiation.
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science & Technology of China, Hefei, Anhui 230027, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science & Technology of China, Hefei, Anhui 230027, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Dabao
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Dingqing
authors.initials: D
authors.lastname: Feng
authors.affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ling
authors.affiliation: Departments of Oncological Radiotherapy, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science & Technology of China, Hefei, Anhui 230027, China. Electronic address: xiaow@ustc.edu.cn.
authors.forename: Weihua
authors.initials: W
authors.lastname: Xiao
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science & Technology of China, Hefei, Anhui 230027, China. Electronic address: shange@ustc.edu.cn.
authors.forename: Ge
authors.initials: G
authors.lastname: Shan
authors.affiliation: Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China. Electronic address: caddie1234@gmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D064448:Filamins / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000235:genetics*; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D064448:Filamins; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D062085:RNA, Long Noncoding; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D042943:Dihydrouracil Dehydrogenase (NADP)
keywords: Cervical cancer; LIF; LncRNA; ΔNp63α