title: High expression of IMPDH2 is associated with aggressive features and poor prognosis of primary nasopharyngeal carcinoma.
abstract: Inosine monophosphate dehydrogenase type II (IMPDH2) has been shown to play critical roles in the development and progression of several human cancers. However, little is known about IMPDH2 expression and its clinical significance in nasopharyngeal carcinoma (NPC). Western blotting, qRT-PCR and immunohistochemistry were employed to evaluate IMPDH2 expression in NPC cell lines and tissues. In our study, elevated expression of IMPDH2 was observed at both the protein and mRNA levels in NPC cell lines than in NPEC2 Bmi-1. IMPDH2 protein expression was markedly higher in NPC tissues than in adjacent non-tumorous tissues. Moreover, IMPDH2 expression in NPC correlated with several clinicopathological parameters, including T classification (P = 0.023), TNM stage (P = 0.020), distant metastasis (P = 0.001) and death (P = 0.002). Further Cox regression analysis suggested that IMPDH2 expression was an independent prognostic factor for overall survival (P = 0.001) and disease-free survival (P < 0.001). In addition, stratified survival analysis showed that high expression of IMPDH2 could be a prognostic factor for NPC patients with TNM stage I/II (OS: P = 0.012; DMFS: P = 0.007), TNM stage III/IV (OS: P = 0.028; DMFS: P = 0.020). Our study demonstrates IMPDH2 may be served as an independent prognostic biomarker for NPC patients, in which high IMPDH expression suggests poor prognosis of NPC patients.
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.|Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.
authors.forename: Zhousan
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Duan
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Extracorporeal Circulation, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Rong
authors.affiliation: Department of Surgical Laboratory, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.
authors.forename: Kebing
authors.initials: K
authors.lastname: Wang
authors.affiliation: Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, China.|Department of Ultrasound, Cancer Center, Sun Yat-Sen University, Guangzhou, 510060, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yun
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China.
authors.forename: Huiwen
authors.initials: H
authors.lastname: Weng
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China. yes20111212@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Ye
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China. zjxlundy@hotmail.com.|Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, China. zjxlundy@hotmail.com.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor; D002277:Carcinoma / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D006801:Humans; D007168:IMP Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D012372:ROC Curve; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D007168:IMP Dehydrogenase; C488211:IMPDH1 protein, human; C501687:IMPDH2 protein, human