title: Micro/extended-nano sampling interface from a living single cell.
abstract: Single-cell analysis is of increasing importance in many fields, but is challenging due to the ultra-small volumes (picoliters) of single cells. Indeed, analysis of a specific analyte might require the analysis of a single molecule or several molecules. Analytical processes usually include sampling, chemical processing, and detection. Although several papers have reported chemical processing and detection methods for single cells, a sampling method compatible with maintaining the viability of a single cell during sampling has yet to be developed. Here, we propose a femtoliter sampling method from a living single cell using micro/nanofluidic device technology. The sampling of 39 fL of cytoplasm from a single human aortic endothelial cell was demonstrated and its viability after sampling was confirmed.
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. kitamori@icl.t.u-tokyo.ac.jp.
authors.forename: L
authors.identifier: http://orcid.org/0000-0002-0117-7065
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. kitamori@icl.t.u-tokyo.ac.jp.
authors.forename: K
authors.identifier: http://orcid.org/0000-0001-7232-5531
authors.initials: K
authors.lastname: Mawatari
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. kitamori@icl.t.u-tokyo.ac.jp.
authors.forename: K
authors.identifier: http://orcid.org/0000-0002-9163-9373
authors.initials: K
authors.lastname: Morikawa
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. kitamori@icl.t.u-tokyo.ac.jp.
authors.forename: Y
authors.identifier: http://orcid.org/0000-0001-9265-8237
authors.initials: Y
authors.lastname: Pihosh
authors.affiliation: Department of Dermatology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan.
authors.forename: A
authors.identifier: http://orcid.org/0000-0002-8194-9140
authors.initials: A
authors.lastname: Yoshizaki
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. kitamori@icl.t.u-tokyo.ac.jp.
authors.forename: T
authors.identifier: http://orcid.org/0000-0003-2969-6550
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D036103:Nanotechnology; D059010:Single-Cell Analysis