title: Genetic polymorphisms of NFκB1-94ins/delATTG and NFκBIA-881A/G genes in Egyptian patients with colorectal cancer.
abstract: To assess the association of genetic polymorphisms of NFκB1 and NFκBIA genes with the susceptibility to colorectal cancer (CRC). Subjects included 100 Egyptian patients with CRC (60 males and 40 females) in addition to 85 healthy controls (47 males and 38 females) from the same locality. For all participants, genetic polymorphisms of NFκB1-94ins/delATTG (rs28362491) and NFκBIA-881A/G (rs3138053) were detected by using restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). CRC patients showed a significantly higher frequency of the NFκB1-94ins/ins genotype than controls (30 vs. 4.7%) that was significant in the recessive (OR 17.69, 95% CI   5.41-57.82, p < 0.0001) and codominant models (OR   18.28, 95% CI   4.87-68.6, p < 0.0001). The NFκB1-94ins allele frequency was significantly higher among patients than controls (58 vs. 39%, OR  2.18, 95% CI  1.4-3.3, p = 0.0004). We also noticed that the genotype G/G of NFκBIA-881 polymorphism was present in patients (4%) while it was absent (0%) in controls with increased frequency of the NFκBIA-881G allele in patients compared to controls (23 vs. 14%, p = 0.041). These polymorphisms were more associated with smoking and advanced tumor staging. This study indicates that the NFκB1-94ins/ins genotype was associated with the risk of developing colorectal cancer in Egyptian subjects. Also, CRC cases showed an increase in the frequency of NFκBIA-881G allele but not reaching statistical significance for multiple comparisons.
authors.affiliation: Physiology Department, Faculty of Science, Tanta University, Tanta, Egypt. Mohamed.youssef082@gmail.com.
authors.forename: Mohamed Ragab
authors.identifier: 0000-0002-9183-008X
authors.initials: MR
authors.lastname: Youssef
authors.affiliation: Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Zeinab Ibraheim
authors.initials: ZI
authors.lastname: Attia
authors.affiliation: Genetics Unit, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Rizk Ahmed
authors.initials: RA
authors.lastname: El-Baz
authors.affiliation: Oncology Center, Mansoura University, Mansoura, Egypt.
authors.forename: Sameh
authors.initials: S
authors.lastname: Roshdy
authors.affiliation: Genetics Unit, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Settin
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D004534:Egypt; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha / Q000235:genetics*; D052002:NF-kappa B p50 Subunit / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha
keywords: Colorectal cancer; Egypt; NFκB1-94ins/delATTG; NFκBIA-881A/G