title: Essential oil composition and antifungal activity of Melissa officinalis originating from north-Est Morocco, against postharvest phytopathogenic fungi in apples.
abstract: To investigate biological control methods against post-harvest phytopathogenic fungi in apples, tests on the antifungal activity of essential oil of Melissa officinalis were carried out. The essential oil, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Analysis of the essential oil was able to detect 88.7% of the components. The main components are P-mentha-1,2,3-triol (13.1%), P-menth-3-en-8-ol (8.8%), pulegone (8.8%), piperitynone oxide (8.4%) and 2-piperitone oxide (7.3%). The determination of the antifungal activity of the essential oil of M. officinalisis carried out in vitro using the technique of poison food (PF) and the volatile activity test (VA). To carry out these two tests, three phytopathogens that cause the deterioration of apples have been selected: Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer. The overall results of this study suggest that M. officinalis essential oil has potential as a bio-antifungal preservative for the control of post-harvest diseases of apple.
authors.affiliation: Laboratoire de chimie analytique appliquée, matériaux et environnement (LC2AME), Faculté des Sciences, B.P.717, 60000 Oujda, Morocco.
authors.forename: Y
authors.initials: Y
authors.lastname: El Ouadi
authors.affiliation: Laboratoire des Substances Naturelles & Synthese et Dynamique Moléculaire, Faculté des Sciences et Techniques, Errachidia, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: Manssouri
authors.affiliation: Laboratoire de chimie analytique appliquée, matériaux et environnement (LC2AME), Faculté des Sciences, B.P.717, 60000 Oujda, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Bouyanzer
authors.affiliation: Laboratoire des Substances Naturelles & Synthese et Dynamique Moléculaire, Faculté des Sciences et Techniques, Errachidia, Morocco.
authors.forename: L
authors.initials: L
authors.lastname: Majidi
authors.affiliation: LCOMPN-URAC25, Faculty of Sciences, Laboratory of Organic Chemistry, Macromolecular and Natural Products, University Mohamed 1st University, BP 524, 60000 Oujda, Morocco.
authors.forename: H
authors.initials: H
authors.lastname: Bendaif
authors.affiliation: Laboratoire de chimie analytique appliquée, matériaux et environnement (LC2AME), Faculté des Sciences, B.P.717, 60000 Oujda, Morocco. Electronic address: h.elmsellem@gmail.com.
authors.forename: H
authors.initials: H
authors.lastname: Elmsellem
authors.affiliation: Research Department, LLC «Science & Education», Russia & Researcher, All Russian Institue of Phtopatology, Moscow Region, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Shariati
authors.affiliation: LCOMPN-URAC25, Faculty of Sciences, Laboratory of Organic Chemistry, Macromolecular and Natural Products, University Mohamed 1st University, BP 524, 60000 Oujda, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Melhaoui
authors.affiliation: Laboratoire de chimie analytique appliquée, matériaux et environnement (LC2AME), Faculté des Sciences, B.P.717, 60000 Oujda, Morocco.
authors.forename: B
authors.initials: B
authors.lastname: Hammouti
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D061046:Biological Control Agents / Q000737:chemistry / Q000494:pharmacology; D020171:Botrytis / Q000187:drug effects / Q000472:pathogenicity; D005520:Food Preservatives; D061353:Food Storage; D005638:Fruit / Q000382:microbiology; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D005659:Fungicides, Industrial; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D027845:Malus / Q000382:microbiology*; D031334:Melissa / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009018:Morocco; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010407:Penicillium / Q000187:drug effects / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D012233:Rhizopus / Q000187:drug effects / Q000472:pathogenicity
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents; D005520:Food Preservatives; D005659:Fungicides, Industrial; D009822:Oils, Volatile
keywords: Antifungal activity; Apple; Essential oil; GC-MS analysis; Melissa officinalis