title: Fluorescent labelling in living cells.
abstract: The labelling of proteins with green fluorescent protein enabled the visualization of proteins in living cells for the first time. Since then, much progress has been made in the field. Modern strategies allow the labelling of proteins in live cells through a range of specialized methods with sophisticated chemical probes that show enhanced photophysical properties compared to fluorescent proteins. This review briefly summarizes recent advances in the field of fluorescent chemical protein labelling inside living cells and illustrates key aspects on the requirements and advantages of each given method.
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP), Campus Berlin-Buch, Robert-Roessle-Str. 10, D-13125 Berlin, Germany.
authors.forename: Anselm Fabian Lowell
authors.initials: AFL
authors.lastname: Schneider
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP), Campus Berlin-Buch, Robert-Roessle-Str. 10, D-13125 Berlin, Germany; Humboldt-Universität zu Berlin, Institut für Chemie, Brook-Taylor-Str. 2, D-12489 Berlin, Germany. Electronic address: hackenbe@fmp-berlin.de.
authors.forename: Christian Peter Richard
authors.initials: CPR
authors.lastname: Hackenberger
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D049452:Green Fluorescent Proteins