title: SINAT E3 Ligases Control the Light-Mediated Stability of the Brassinosteroid-Activated Transcription Factor BES1 in Arabidopsis.
abstract: The plant hormones brassinosteroids (BRs) participate in light-mediated regulation of plant growth, although the underlying mechanisms are far from being fully understood. In addition, the function of the core transcription factor in the BR signaling pathway, BRI1-EMS-SUPPRESSOR 1 (BES1), largely depends on its phosphorylation status and its protein stability, but the regulation of BES1 is not well understood. Here, we report that SINA of Arabidopsis thaliana (SINATs) specifically interact with dephosphorylated BES1 and mediate its ubiquitination and degradation. Our genetic data demonstrated that SINATs inhibit BR signaling in a BES1-dependent manner. Interestingly, we found that the protein levels of SINATs were decreased in the dark and increased in the light, which changed BES1 protein levels accordingly. Thus, our study not only uncovered a new mechanism of BES1 degradation but also provides significant insights into how light conditionally regulates plant growth through controlling accumulation of different forms of BES1.
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Center of Integrative Biology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Mengran
authors.initials: M
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Center of Integrative Biology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yinmeng
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Trevor
authors.initials: T
authors.lastname: Nolan
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
authors.forename: Guiliang
authors.initials: G
authors.lastname: Tang
authors.affiliation: Center of Integrative Biology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xlwang@mail.hzau.edu.cn.
authors.forename: Xuelu
authors.initials: X
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D060406:Brassinosteroids / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D055785:Gene Knockdown Techniques; D008027:Light; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000528:radiation effects; D055550:Protein Stability / Q000187:drug effects / Q000528:radiation effects; D059748:Proteolysis / Q000187:drug effects / Q000528:radiation effects; D054829:RING Finger Domains; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination / Q000187:drug effects / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; C456160:BES1 protein, Arabidopsis; D060406:Brassinosteroids; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D044767:Ubiquitin-Protein Ligases
keywords: BES1; SINAT; brassinosteroids; degradation; hypocotyl elongation; light