title: Cofilin 1 induces the epithelial-mesenchymal transition of gastric cancer cells by promoting cytoskeletal rearrangement.
abstract: Epithelial-mesenchymal transition (EMT) is an important biological process whereby malignant tumor cells obtain the ability to migrate, invade, resist apoptosis and degrade the extracellular matrix. We found that Cofilin1 (CFL1) expression was elevated in clinical gastric cancer specimens and correlated with biomarkers of EMT in BGC-823 gastric cancer cells. BGC-823 cells exhibited EMT phenotypes and increased metastatic ability when induced by TGF-β1. By contrast, BGC-823 cells transfected with Lv-siRNA-CFL1 did not exhibit EMT phenotypes under the same inducing conditions. As CFL1 expression increased, EMT cell filopodia stretched out. In addition, the ultrastructures observed using transmission electron microscopy indicated that silencing of CFL1 markedly inhibited depolymerization of fibrous actin and cytoskeletal reorganization during EMT. Similar results were obtained in vivo. These findings demonstrate that CFL1 induces EMT by promoting cytoskeletal rearrangement. Our results may provide the basis for developing new anticancer drugs to inhibit CFL1.
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.|Clinical Medicine College of Yangzhou University, Yangzhou 225000, China.|The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.|The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.
authors.forename: Lide
authors.initials: L
authors.lastname: Tao
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.|Clinical Medicine College of Yangzhou University, Yangzhou 225000, China.|The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.affiliation: The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Gu
authors.affiliation: The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Dai
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.|Clinical Medicine College of Yangzhou University, Yangzhou 225000, China.|The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Tengyang
authors.initials: T
authors.lastname: Ni
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.|Clinical Medicine College of Yangzhou University, Yangzhou 225000, China.|The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Ding
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Xiao
authors.affiliation: The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Qian
authors.affiliation: The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225000, China.|Clinical Medicine College of Yangzhou University, Yangzhou 225000, China.|The State Administration of Traditional Chinese Medicine Key Laboratory of Toxic Pathogens-Based Therapeutic Approaches to Gastric Cancer, Yangzhou 225000, China.|Institution of Combining Chinese Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou 225000, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D049109:Cell Proliferation; D051338:Cofilin 1 / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D011379:Prognosis; D015398:Signal Transduction; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D053773:Transforming Growth Factor beta1; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; C495810:CFL1 protein, human; D051338:Cofilin 1; D053773:Transforming Growth Factor beta1
keywords: CFL1; cytoskeleton rearrangement; epithelial-mesenchymal transition (EMT); gastric cancer (GC)