title: Increased circulating leukocyte-derived microparticles in ischemic cerebrovascular disease.
abstract: OBJECTIVE
  Circulating leukocyte-derived microparticles act as proinflammatory mediators that reflect vascular inflammation. In this study, we examined the hypothesis that the quantity of leukocyte-derived microparticles is increased in patients with ischemic cerebrovascular diseases, and investigated utility of various phenotypes of leukocyte-derived microparticles as specific biomarkers of vascular inflammation injury. Additionally we focused on identifying leukocyte-derived microparticles that may be correlated with stroke severity in acute ischemic stroke patients.
  
  
  METHODS
  The plasma concentration of leukocyte-derived microparticles obtained by a series of centrifugations of 76 consecutive patients with ischemic cerebrovascular diseases and 70 age-, sex-, and race-matched healthy controls were determined by flow cytometry.
  
  
  RESULTS
  Significantly elevated numbers of leukocyte (CD45+), monocyte (CD14+), lymphocyte (CD4+), granulocyte (CD15+) derived microparticles were found in the plasma samples of patients ischemic cerebrovascular diseases, compared to healthy controls (p<0.05). Furthermore, the plasma levels of CD14+ microparticles were significantly correlated with stroke severity (r=0.355, p=0.019), cerebral vascular stenosis severity (r=0.255, p=0.025) and stroke subtype (r=0.242, p=0.036). No association with stroke was observed for other leukocyte-derived phenotypes.
  
  
  CONCLUSIONS
  These results demonstrate that circulating leukocyte-derived microparticles amounts are increased in patients with ischemic cerebrovascular diseases, compared with healthy controls. As proinflammatory mediators, leukocyte-derived microparticles may contribute to vascular inflammatory and the inflammatory process in acute ischemic stroke. Levels of CD14+ microparticles may be a promising biomarker of ischemic severity and outcome of stroke in the clinic.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Guangxi Medical University, No. 22, Shuang Yong Road, Nanning 530021, Guangxi, China.
authors.forename: Zhangping
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Neurology, First Affiliated Hospital, Guangxi Medical University, No. 22, Shuang Yong Road, Nanning 530021, Guangxi, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Neurology, First Affiliated Hospital, Guangxi Medical University, No. 22, Shuang Yong Road, Nanning 530021, Guangxi, China. Electronic address: hmdqc6639@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Qin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002545:Brain Ischemia / Q000097:blood* / Q000150:complications / Q000473:pathology*; D015704:CD4 Antigens / Q000032:analysis / Q000097:blood; D055252:Cell-Derived Microparticles / Q000473:pathology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000150:complications / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000097:blood; D007962:Leukocytes / Q000473:pathology*; D016256:Lewis X Antigen / Q000032:analysis / Q000097:blood; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D015704:CD4 Antigens; D016256:Lewis X Antigen; D018950:Lipopolysaccharide Receptors; D017493:Leukocyte Common Antigens
keywords: Ischemic cerebrovascular disease; Leukocyte-derived Microparticles; Vascular inflammatory