title: Proliferation and maturation of intratumoral blood vessels in women with malignant ovarian tumors assessed with cancer stem cells marker nestin and platelet derived growth factor PDGF-B.
abstract: Platelet-derived growth factor B (PDGF-B) and nestin have been suggested to be useful in the assessment of neoangiogenesis in malignant ovarian masses. We aimed to investigate a possible association of these markers with newly formed microcapillaries and perivascular cells in ovarian tumors.
  
  
  
  Microvessel density (MVD) and pericytes were studied in 82 women with ovarian neoplasms, including 7 benign cysts, 7 borderline masses, 64 epithelial ovarian cancers and 4 other malignant ovarian tumors. Immunohistochemical staining included antibodies to CD34, PDGF-B and nestin.
  
  
  
  Median values of CD34-positive and nestin-positive MVD were: 24,5 (range:17-32) and 21 (range: 12-31), respectively. No significant correlation between intratumoral CD-34 positive MVD and nestin-positive MVD was found. Benign and borderline lesions more frequently than malignant tumors displayed low or medium values of nestin-positive MVD (p = 0.01). Histological grading of malignant tumors was associated with nestin-positive MVD (p = 0.01). Nestin expression in tumor cells was not correlated with tumor grade or histological subtype. PDGF-B expression was found in tumor microves-sels in 72% of cases (59/82). High expression of PDGF in pericapillary cells was strongly associated with high expression of this marker in cancer cells (p = 0.007). Significant correlations between PDGF-B and nestin expression in malignant tumor microvessels were also found (p = 0.04). Nestin and PDGF-B expressions were strongly associated with high grade tumors when compared to low grade or benign masses.
  
  
  
  We conclude that the assessment of PDGF-B and nestin-positive MVD could be used to identify only highly active, angiogenic malignant ovarian masses, where tumor vasculature is formed.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Czekierdowska
authors.forename: Norbert
authors.initials: N
authors.lastname: Stachowicz
authors.forename: Mieczysław
authors.initials: M
authors.lastname: Chróściel
authors.affiliation: Department of Gynecological Oncology and Gynecology, Medical University of Lublin, Poland. a.czekierdowski@gmail.com.
authors.forename: Artur
authors.initials: A
authors.lastname: Czekierdowski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D000077216:Carcinoma, Ovarian Epithelial; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D055806:Microvessels / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D064231:Nestin / Q000378:metabolism*; D010048:Ovarian Cysts / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D020286:Pericytes / Q000473:pathology*; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; C577484:NES protein, human; D064231:Nestin; D020574:Proto-Oncogene Proteins c-sis
keywords: PDGF; microvessel desity; nestin; ovarian cancer; pericytes