title: Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015).
abstract: Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Orf virus (ORFV) infection was confirmed by PCR amplification of viral DNA (RNA polymerase, B2L and virus interferon-resistance genes) in clinical samples. Some detected genes were sequenced and phylogenetically analyzed. This is the first report on molecular characterization of ORFV in goats in Zambia.
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia. esikabala@yahoo.com.
authors.forename: Edgar
authors.identifier: http://orcid.org/0000-0001-9423-0816
authors.initials: E
authors.lastname: Simulundu
authors.affiliation: Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.
authors.forename: Nandi
authors.initials: N
authors.lastname: Mtine
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Thoko F
authors.initials: TF
authors.lastname: Kapalamula
authors.affiliation: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kajihara
authors.affiliation: Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: James
authors.initials: J
authors.lastname: Ngoma
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Victor
authors.initials: V
authors.lastname: Zulu
authors.affiliation: Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Kwenda
authors.affiliation: Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.
authors.forename: Chrispin
authors.initials: C
authors.lastname: Chisanga
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Isaac K
authors.initials: IK
authors.lastname: Phiri
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.|Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.|Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan.
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Mweene
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D004474:Ecthyma, Contagious / Q000453:epidemiology / Q000821:virology*; D015511:Goat Diseases / Q000453:epidemiology / Q000821:virology*; D006041:Goats; D059014:High-Throughput Nucleotide Sequencing; D058751:Livestock / Q000821:virology; D009923:Orf virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015047:Zoonoses / Q000453:epidemiology / Q000821:virology
chemical_list: D004279:DNA, Viral