title: Malignant canine mammary tumours: Preliminary genomic insights using oligonucleotide array comparative genomic hybridisation analysis.
abstract: Neoplastic mammary disease in female dogs represents a major health concern for dog owners and veterinarians, but the genomic basis of the disease is poorly understood. In this study, we performed high resolution oligonucleotide array comparative genomic hybridisation (oaCGH) to assess genome wide DNA copy number changes in 10 malignant canine mammary tumours from seven female dogs, including multiple tumours collected at one time from each of three female dogs. In all but two tumours, genomic imbalances were detected, with losses being more common than gains. Canine chromosomes 9, 22, 26, 27, 34 and X were most frequently affected. Dissimilar oaCGH ratio profiles were observed in multiple tumours from the same dogs, providing preliminary evidence for probable independent pathogenesis. Analysis of adjacent samples of one tumour revealed regional differences in the number of genomic imbalances, suggesting heterogeneity within tumours.
authors.affiliation: Department of Microscopy, Laboratory of Histology and Embryology, Institute of Biomedical Sciences Abel Salazar, ICBAS - UPorto, University of Porto, Porto, Portugal. Electronic address: mssantos@icbas.up.pt.
authors.forename: Marta
authors.initials: M
authors.lastname: Santos
authors.affiliation: Department of Pathology and Molecular Immunology, ICBAS - UPorto, Porto, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Dias-Pereira
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine North Carolina State University, Raleigh, NC, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Williams
authors.affiliation: Department of Pathology and Molecular Immunology, ICBAS - UPorto, Porto, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lopes
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine North Carolina State University, Raleigh, NC, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA; Cancer Genetics, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Breen
mesh_terms: D000818:Animals; D055028:Comparative Genomic Hybridization / Q000662:veterinary*; D056915:DNA Copy Number Variations; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000473:pathology
keywords: Array comparative genomic hybridisation; Canine mammary tumours; Chromosome; Genetics