title: Serum Th1 and Th17 related cytokines and autoantibodies in patients with Posner-Schlossman syndrome.
abstract: Posner-Schlossman syndrome (PSS) shares some clinical features with uveitis and open angle glaucoma. Cytokines and autoantibodies have been associated with uveitis and open angle glaucoma. However, the role of serum cytokines and autoantibodies in the pathogenesis of PSS remains unknown. This study aimed to evaluate the associations of type 1 T helper (Th1) and Th17 related cytokines and autoantibodies with PSS. Peripheral blood serum samples were collected from 81 patients with PSS and 97 gender- and age-matched healthy blood donors. Th1 and Th17 related cytokines, including interleukin-1β (IL-1β), IL-12, tumor necrosis factor-α (TNF-α), interferon- γ (IFN-γ), IL-6 and IL-17, and glucose-6-phosphate isomerase (GPI) were determined by double antibody sandwich ELISA. Anti-nuclear antibody (ANA), anti-keratin antibody (AKA) and anti-neutrophil cytoplasmic antibody (ANCA) were detected by indirect immunofluorescence assay. Anti-cardiolipin antibody (ACA)-IgG, ACA-IgM, ACA-IgA, anti-double stranded DNA (anti-dsDNA) and anti-cyclic citrullinated peptide antibody (anti-CCP) were detected by indirect ELISA. Serum levels of IL-1β, IL-12 and IL-6 in PSS patients were significantly lower than those in controls (P < 0.003), and these associations survived the Bonferroni correction (Pc < 0.018). There was no significant difference in serum levels of TNF-α, IFN-γ and IL-17 between the PSS and control groups (Pc > 0.12). Positive rate of serum anti-dsDNA in PSS patients was significantly higher than that in the control group (P = 0.002, Pc = 0.018), while positive rates of serum ANA, AKA, ANCA, ACA-IgG, ACA-IgM, ACA-IgA, GPI and anti-CCP in the PSS group were not significantly different from those in the control group (Pc > 0.09). These results suggest that anti-dsDNA may contribute to the pathogenesis of PSS, while Th1 and Th17 related cytokines and other autoantibodies may not be major contributors to PSS.
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong, China.|School of Ophthalmology & Optometry Affiliated to Shenzhen University, Shenzhen, Guangdong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong, China.
authors.forename: Wenchieh
authors.initials: W
authors.lastname: Chen
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong, China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Huang
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Peng
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong, China.
authors.forename: Tianhui
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Deng
authors.affiliation: School of Ophthalmology & Optometry Affiliated to Shenzhen University, Shenzhen, Guangdong, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liang
authors.affiliation: Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen, Guangdong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States of America.
authors.forename: Bao Jian
authors.identifier: http://orcid.org/0000-0002-6851-2737
authors.initials: BJ
authors.lastname: Fan
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D013577:Syndrome; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014605:Uveitis / Q000097:blood* / Q000276:immunology
chemical_list: D001323:Autoantibodies; D016207:Cytokines