title: Zika virus detected in amniotic fluid and umbilical cord blood in an in vitro fertilization-conceived pregnancy in Venezuela.
abstract: To describe the consequences of Zika virus infection at 10 weeks of gestation in an IVF-conceived pregnancy in Venezuela.
  
  
  
  A case report.
  
  
  
  Private assisted reproduction unit.
  
  
  
  A 36-year-old patient who conceived her first pregnancy through IVF and became infected with Zika virus at 10 weeks' gestation in Venezuela.
  
  
  
  In vitro fertilization with fresh ET. Clinical, laboratory, and imaging Zika diagnostic methods.
  
  
  
  Zika virus detection by real-time polymerase chain reaction (PCR) in maternal plasma, PCR in amniotic fluid and umbilical cord blood. Ultrasonography findings of anatomic abnormalities.
  
  
  
  Zika infection was confirmed at 10 weeks' gestation by real-time PCR; ultrasound results appeared normal. At 19 weeks' gestation, an ultrasound revealed biometry on three SDs below the means for all parameters but with no apparent anatomic abnormality. Zika virus was positive in maternal urine and amniotic fluid by PCR at 19 weeks' gestation. Ultrasound at 21 weeks + 4 days of gestation showed fetal cerebellar hypoplasia with ventricular dysmorphia, particularly marked on the left, consistent with microcephaly and ventriculomegaly. Because of the poor prognosis, pregnancy was interrupted at 24 weeks' gestation, in France. The PCR in umbilical cord blood taken in this procedure was positive for Zika virus.
  
  
  
  Initial ultrasound findings in pregnancy may not be informative. Only at 21 weeks + 4 days of gestation did an ultrasound reveal fetal microcephaly and ventriculomegaly. Combined clinical, laboratory, and imaging findings provided a complete picture of the severe damage caused by Zika infection.
authors.affiliation: Fertility Unit, Unifertes, Clinica El Avila, Caracas, Venezuela. Electronic address: ibenjaminf@gmail.com.
authors.forename: Isaac
authors.initials: I
authors.lastname: Benjamin
authors.affiliation: Fertility Unit, Unifertes, Clinica El Avila, Caracas, Venezuela.
authors.forename: Gissel
authors.initials: G
authors.lastname: Fernández
authors.affiliation: Fertility Unit, Unifertes, Clinica El Avila, Caracas, Venezuela.
authors.forename: José Valentin
authors.initials: JV
authors.lastname: Figueira
authors.affiliation: Fertility Unit, Unifertes, Clinica El Avila, Caracas, Venezuela.
authors.forename: Leticia
authors.initials: L
authors.lastname: Parpacén
authors.affiliation: Fertility Unit, Unifertes, Clinica El Avila, Caracas, Venezuela.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Urbina
authors.affiliation: Fertility Unit, Unifertes, Clinica El Avila, Caracas, Venezuela.
authors.forename: Randolfo
authors.initials: R
authors.lastname: Medina
mesh_terms: D000028:Abortion, Induced; D000328:Adult; D000653:Amniotic Fluid / Q000821:virology; D005260:Female; D005307:Fertilization in Vitro; D005312:Fetal Blood / Q000821:virology; D006801:Humans; D006849:Hydrocephalus / Q000821:virology*; D007246:Infertility / Q000628:therapy / Q000821:virology; D008297:Male; D008431:Maternal-Fetal Exchange; D008831:Microcephaly / Q000821:virology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D016896:Treatment Outcome; D014687:Venezuela; D000071244:Zika Virus / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000821:virology*
keywords: Zika virus; amniotic fluid; congenital malformations; microcephaly; ventriculomegaly