title: [Over-expression of uracil DNA glycosylase 2 (UNG2) enhances the resistance to oxidative damage in HepG2 cells].
abstract: Objective To investigate the uracil glycosidic enzyme activity of uracil DNA glycosylase 2 (UNG2) and study the role of UNG2 in the resistance of antioxidant stress of HepG2 cells. Methods The UNG2-expressing vector was built. Western blotting was used to detect the expression of UNG2. Immunofluorescence staining was performed to observe the cellular location of UNG2. Oligonucleotide was used as substrate for the determination of the UNG2 glycosidic enzyme activity. H2O2toxicity assay was done to study the function of UNG2 in the antioxidant resistance of hepatocellular carcinoma HepG2 cells. Results UNG2 was successfully over-expressed in HEK293FT cells, and UNG2 was found to be mainly located in nucleus. Enzyme activity assay showed that UNG2 had significant oligonucleotide dU glycosidic enzyme activity. H2O2toxicity assay showed that over-expressed UNG2 could remarkably increase the survival of HepG2 cells after exposed to H2O2. Conclusion UNG2 possesses specific DNA glycosidic enzyme activity, and it can protect HepG2 cells against oxidative stress damage.
authors.affiliation: Institute of Genetics Diagnostic Technology, Pharmacy, Department of Pharmacy, Central Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032; Department of Blood Transfusion, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Cao
authors.affiliation: Institute of Genetics Diagnostic Technology, Pharmacy, Department of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Institute of Genetics Diagnostic Technology, Pharmacy, Department of Pharmacy, Fourth Military Medical University, Xi'an 710032, China; Department of Blood Transfusion, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Institute of Genetics Diagnostic Technology, Pharmacy, Department of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: gyanhai@fmmu.edu.cn.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Central Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: huangxf@fmmu.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Huang
mesh_terms: D002477:Cells / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D006861:Hydrogen Peroxide; C492570:CCNO protein, human; D045647:DNA Glycosylases