title: Genotoxicity study of nickel oxide nanoparticles in female Wistar rats after acute oral exposure.
abstract: Nanoparticles (NPs) apart from their widespread advantages and increased utilisation, have aroused concerns over their safe use. Nickel (II) oxides (NiO) NPs are used as catalysts, biosensors and in many of the consumer products. The increasing use of NiO NPs necessitates an improved understanding of their potential impact on the environment and human health. In this study, we investigated the acute genotoxic effects of NiO NPs by oral route administration with three different doses (125, 250 and 500 mg/kg bw). Before the in vivo toxicological evaluation, characterisation of particles by Transmission Electron Microscopy, X-ray diffraction, Dynamic Light Scattering (DLS) and Laser Doppler Velocimetry analysis was performed. Genotoxicity biomarkers such as comet, micronucleus and chromosomal aberrations (CAs) assays were utilised in this study. To document the uptake, retention and elimination of the NPs, biodistribution studies were also performed. The particle size obtained from Transmission Electron Microscopy analysis for NiO NPs was 15.62 ± 2.59 nm. The mean hydrodynamic diameter and PdI of NiO NPs in Milli-Q water suspension obtained by DLS was 168.9 ± 17.13 nm and 0.375, respectively. Comet assay revealed significant (P < 0.001) DNA damage at 500 mg/kg bw dose in the PBL, liver and kidney cells of rats at the 24-h sampling time. The result of micronucleus and CAs tests was in agreement with the comet assay data. Biodistribution of NiO NPs revealed a maximum accumulation of Ni in the liver tissue at the 24-h sampling time. Our study showed significant DNA damage at the high dose level and the effect was more prominent at 24-h sampling time, providing preliminary evidence that the NiO NPs are capable of inducing genotoxicity when administered through the oral route. However, mechanistic investigations are needed before drawing any firm conclusion regarding the toxicology of NiO NPs.
authors.affiliation: Toxicology Unit, Biology Division.|Academy of Scientific and Innovative Research, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana 500007, India.
authors.forename: Naresh
authors.initials: N
authors.lastname: Dumala
authors.affiliation: Toxicology Unit, Biology Division.|Academy of Scientific and Innovative Research, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana 500007, India.
authors.forename: Bhanuramya
authors.initials: B
authors.lastname: Mangalampalli
authors.affiliation: Toxicology Unit, Biology Division.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Chinde
authors.affiliation: Toxicology Unit, Biology Division.
authors.forename: Srinivas Indu
authors.initials: SI
authors.lastname: Kumari
authors.affiliation: Toxicology Unit, Biology Division.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mahoob
authors.affiliation: Toxicology Unit, Biology Division.
authors.forename: Mohammad Fazlur
authors.initials: MF
authors.lastname: Rahman
authors.affiliation: Toxicology Unit, Biology Division.|Academy of Scientific and Innovative Research, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana 500007, India.
authors.forename: Paramjit
authors.initials: P
authors.lastname: Grover
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002869:Chromosome Aberrations / Q000139:chemically induced; D004249:DNA Damage; D005260:Female; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D009532:Nickel / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D010316:Particle Size; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D009153:Mutagens; D009532:Nickel; C028007:nickel monoxide