title: Cylindrospermopsin effects on cell viability and redox milieu of Neotropical fish Hoplias malabaricus hepatocytes.
abstract: Cylindrospermopsin (CYN) is a cyanotoxin that is cytotoxic to a wide variety of cells, particularly to the hepatocytes. In this study, the toxic effects of purified CYN were investigated in primary cultured hepatocytes of Neotropical fish Hoplias malabaricus. After isolation, attachment, and recovery for 72 h, the cells were exposed for 72 h to 0, 0.1, 1.0, 10, and 100 μg l-1of CYN. Then, cell viability and a set of oxidative stress biomarker responses were determined. Catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, and glutathione S-transferase activities were not affected by exposure to CYN. Concentration-dependent decrease of glutathione reductase activity occurred for most CYN-exposed groups, whereas non-protein thiol content increased only for the highest CYN concentration. Lipid peroxidation, protein carbonylation, and DNA damage levels were not altered, but reactive oxygen species levels increased in the cells exposed to the highest concentration of CYN. Cell viability decreased in all the groups exposed to CYN. Thus, CYN may cause a slight change in redox balance, but it is not the main cause of cell death in H. malabaricus hepatocytes.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21949-900, Brazil.|Departamento de Biologia Estrutural, Molecular e Genética. Bloco M. Setor de Ciências Biológicas e da Saúde, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil, CEP 84030-900.
authors.forename: R C
authors.initials: RC
authors.lastname: Silva
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, Curitiba, PR, 81531-990, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Liebel
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, Curitiba, PR, 81531-990, Brazil.
authors.forename: H H P
authors.initials: HHP
authors.lastname: de Oliveira
authors.affiliation: Departamento de Genética, Universidade Federal do Paraná, Caixa Postal 19031, Curitiba, PR, 81531-990, Brazil.
authors.forename: W A
authors.initials: WA
authors.lastname: Ramsdorf
authors.affiliation: Campus Laranjeiras do Sul, Universidade Federal da Fronteira Sul, Laranjeiras do Sul, PR, 85303-775, Brazil.
authors.forename: J R E
authors.initials: JRE
authors.lastname: Garcia
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21949-900, Brazil.
authors.forename: S M F O
authors.initials: SMFO
authors.lastname: Azevedo
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21949-900, Brazil. valeria@biof.ufrj.br.
authors.forename: V F
authors.initials: VF
authors.lastname: Magalhães
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, Curitiba, PR, 81531-990, Brazil. ciro@ufpr.br.
authors.forename: C A
authors.initials: CA
authors.lastname: Oliveira Ribeiro
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, Curitiba, PR, 81531-990, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Filipak Neto
mesh_terms: D000470:Alkaloids; D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D060125:Characiformes; D000087522:Cyanobacteria Toxins; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D014498:Uracil / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D015415:Biomarkers; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil
keywords: Cyanotoxin; Effects; In vitro; Neotropical fish; Oxidative stress