title: Mitochondria Targeted Nanoscale Zeolitic Imidazole Framework-90 for ATP Imaging in Live Cells.
abstract: Zeolitic imidazole frameworks (ZIFs) are an emerging class of functional porous materials with promising biomedical applications such as molecular sensing and intracellular drug delivery. We report herein the first example of using nanoscale ZIFs (i.e., ZIF-90), self-assembled from Zn2+and imidazole-2-carboxyaldehyde, to target subcellular mitochondria and image dynamics of mitochondrial ATP in live cells. Encapsulation of fluorescent Rhodamine B (RhB) into ZIF-90 suppresses the emission of RhB, while the competitive coordination between ATP and the metal node of ZIF-90 dissembles ZIFs, resulting in the release of RhB for ATP sensing. With this method, we are able to image mitochondrial ATP in live cells and study the ATP level fluctuation in cellular glycolysis and apoptosis processes. The strategy reported here could be further extended to tune nanoscale ZIFs inside live cells for targeted delivery of therapeutics to subcellular organelles for advanced biomedical applications.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Deng
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Lanqun
authors.identifier: 0000-0001-8286-9321
authors.initials: L
authors.lastname: Mao
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry* / Q000494:pharmacology; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D017641:Zeolites / Q000737:chemistry* / Q000494:pharmacology; D015032:Zinc / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D007093:Imidazoles; D017641:Zeolites; C029899:imidazole; D000255:Adenosine Triphosphate; D015032:Zinc