title: Keto analogues and amino acids supplementation induces a decrease of white blood cell counts and a reduction of muscle damage during intense exercise under thermoneutral conditions.
abstract: This study evaluated the acute effect of keto analogue and amino acid (AA-KAAA) supplementation on both white blood cell counts and the established biomarkers of muscle damage during exercise under thermoneutral conditions. Sixteen male cyclists received a ketogenic diet for two days and were divided into two equal groups: a group taking AA-KAAA (KA) or a control group (PL). The athletes performed a two hour cycling session followed by a maximum incremental test until voluntary exhaustion (VExh). Blood samples were obtained at rest and during exercise for further hematological and biochemical analyses. Exercise-induced ammonemia increased in the PL group at VExh (75%) but remained unchanged in the KA group. Both groups exhibited a significant increase in leukocyte and neutrophil counts of ∼85% (∼13 × 109L-1), but the shape of the lymphocytes and the eosinophil counts suggest that AA-KAAA supplementation helps prevent lymphocytosis. AA-KAAA supplementation induced a decrease in creatine kinase and aspartate aminotransferase levels at VExh while showing a significant decrease in lactate dehydrogenase at 120 min. We found that AA-KAAA supplementation decreases both the lymphocyte count response in blood and the established biomarkers of muscle damage after intense exercise under a low heat stress environment.
authors.affiliation: Laboratory for Research in Physical Exercise and Metabolism, Federal University of Alagoas. Campus A.C. Simões, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, CEP 57.072-970, Maceió/AL, Brazil. esprado@cedu.ufal.com.
authors.forename: R C P
authors.initials: RCP
authors.lastname: Lima
authors.forename: S R A S
authors.initials: SRAS
authors.lastname: Camerino
authors.forename: T C L
authors.initials: TCL
authors.lastname: França
authors.forename: D S A
authors.initials: DSA
authors.lastname: Rodrigues
authors.forename: M G S
authors.initials: MGS
authors.lastname: Gouveia
authors.forename: A
authors.initials: A
authors.lastname: Ximenes-da-Silva
authors.forename: A
authors.initials: A
authors.lastname: Bassini
authors.forename: E S
authors.initials: ES
authors.lastname: Prado
authors.forename: L C
authors.initials: LC
authors.lastname: Cameron
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D056352:Athletes; D003402:Creatine Kinase; D019587:Dietary Supplements / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D007770:L-Lactate Dehydrogenase; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000293:injuries / Q000378:metabolism*; D055070:Resistance Training
chemical_list: D000596:Amino Acids; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase