title: Characterization of c-Maf+Foxp3-Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells.
abstract: The role of B cells in the development of CD4+regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4+CD25-T cells into CD4+CD25+Foxp3-T cells without additional cytokines or chemicals with regulatory activity and that referred to as Treg-of-B cells. The present study further showed that Treg-of-B cells increased the IL-10-producing population, and the expression of c-Maf, inducible T-cell co-stimulator (ICOS) as well as cytotoxic T-lymphocyte-associated protein 4 (CTLA4) after repeated stimulation of B cells in a cell-cell contact-dependent manner. Long-term cultured Treg-of-B cells exerted IL-10 and CTLA4-mediated antigen-specific suppressive activity; moreover, the single antigen-specific Treg-of-B cells inhibited in a non-antigen-specific fashion. In conclusion, these results suggest that repeated stimulation of B cells induced IL-10-producing CD4+Foxp3-regulatory T cells in a contact-dependent manner and these Treg-of-B cells possess IL-10 and CTLA4-dependent suppressive function.
authors.affiliation: Graduate Institute of Clinical Medicine, National Taiwan University, Taipei City, 10048, Taiwan R.O.C.
authors.forename: Chien-Hui
authors.initials: CH
authors.lastname: Chien
authors.affiliation: Graduate Institute of Clinical Medicine, National Taiwan University, Taipei City, 10048, Taiwan R.O.C.
authors.forename: Hui-Chieh
authors.initials: HC
authors.lastname: Yu
authors.affiliation: Graduate Institute of Clinical Medicine, National Taiwan University, Taipei City, 10048, Taiwan R.O.C.
authors.forename: Szu-Ying
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Graduate Institute of Clinical Medicine, National Taiwan University, Taipei City, 10048, Taiwan R.O.C.|Department of Medical Research, National Taiwan University Hospital, Taipei City, 10002, Taiwan R.O.C.
authors.forename: Bor-Luen
authors.initials: BL
authors.lastname: Chiang
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D051257:Proto-Oncogene Proteins c-maf / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C495608:Maf protein, mouse; D051257:Proto-Oncogene Proteins c-maf