title: Activation of Notch pathway is linked with epithelial-mesenchymal transition in prostate cancer cells.
abstract: Notch signaling has been reported to play an essential role in tumorigenesis. Several studies have suggested that Notch receptors could be oncoproteins or tumor suppressors in different types of human cancers. Emerging evidence has suggested that Notch pathway regulates cell growth, apoptosis, cell cycle, and metastasis. In the current study, we explore whether Notch-1 could regulate the cell invasion and migration as well as EMT (epithelial-mesenchymal transition) in prostate cancer cells. We found that overexpression of Notch-1 enhanced cell migration and invasion in PC-3 cells. However, downregulation of Notch-1 retarded cell migration and invasion in prostate cancer cells. Importantly, we observed that overexpression of Notch-1 led to EMT in PC-3 cells. Notably, we found that EMT-type cells are associated with EMT markers change and cancer stem cell phenotype. Taken together, we concluded that downregulation of Notch-1 could be a promising approach for inhibition of invasion in prostate cancer cells, which could be useful for the treatment of metastatic prostate cancer.
authors.affiliation: a Department of Urology , Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Department of Urology , Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Sha
authors.affiliation: a Department of Urology , Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yang
authors.affiliation: a Department of Urology , Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Xuyuan
authors.initials: X
authors.lastname: Huang
authors.affiliation: a Department of Urology , Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Juanjie
authors.initials: J
authors.lastname: Bo
authors.affiliation: a Department of Urology , Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Huang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D051881:Receptor, Notch1 / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C497761:NOTCH1 protein, human; D051881:Receptor, Notch1
keywords: EMT; Notch-1; invasion; migration; prostate cancer