title: EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans.
abstract: Nicotiana benthamiana ABCG1 and ABCG2 are ABC transporters which are probably involved in the export of capsidiol, the major phytoalexin of Nicotiana species. While capsidiol export by these transporters plays an essential role in post-invasion defense against Phytophthora infestans, they also export unidentified antimicrobial compound(s) involved in pre-invasion defense. In this study, promoter activity of NbABCG2 (Pabcg2a) was analyzed using a GFP marker. Expression of GFP under the control of Pabcg2a was significantly increased by co-expression with the INF1 elicitor from P. infestans. Disruption of the ethylene-responsive GCC box in Pabcg2a compromised INF1-induced activation of Pabcg2a. Consistently, penetration by P. infestans was increased by gene-silencing of NbEIN2, the key ethylene-signaling component, suggesting the involvement of ethylene for pre-invasion defense of N. benthamiana.
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Soriya
authors.initials: S
authors.lastname: Rin
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Mizuno
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Shibata
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Mayuka
authors.initials: M
authors.lastname: Fushimi
authors.affiliation: b Graduate School of Agriculture , Shinshu University , Nagano , Japan.
authors.forename: Shinpei
authors.initials: S
authors.lastname: Katou
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Sato
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Sotaro
authors.initials: S
authors.lastname: Chiba
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Kawakita
authors.affiliation: a Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa, Nagoya , Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Takemoto
mesh_terms: D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D055750:Phytophthora infestans / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; C036216:ethylene
keywords: ABC transporter; GCC box; NbABCG1/2; capsidiol; ethylene signaling; phytoalexin; pre-invasion defense