title: Evaluation of miR-506 and miR-4316 expression in early and non-invasive diagnosis of colorectal cancer.
abstract: OBJECTIVE
  Examination of the entire colon by colonoscopy remains the golden standard for screening of colorectal cancer (CRC). However, patients are reluctant to perform invasive colonoscopies because of interference with their intimacy. Therefore, the potential use of non-invasive analysis of microRNAs expression in liquid biopsy as a novel biomarker for early CRC has investigated in several studies. In this study, we analyzed the expression of two novel microRNAs: miR-506 and miR-4316, which have never been examined in CRC.
  
  
  METHODS
  Plasma samples were collected from 56 patients (median age of 68 years) with operable colorectal cancer and from 70 healthy individuals (median age of 59 years). Expression of plasma microRNAs was evaluated by quantitative reverse transcription polymerase chain reaction using Eco real-time PCR device (Illumina, USA).
  
  
  RESULTS
  We found a significant elevated expression of both examined microRNAs in early CRC patients when compared to those in healthy individuals (p = 0.0054 for miR-506 and p = 0.0025 for miR-4316). The expression of miR-506 and miR-4316 did not depend on gender, age, disease stage, and tumor localization of CRC patients. ROC curve analysis showed that both examined microRNAs could differentiate early stage colorectal cancer from healthy individuals with 76.8% specificity and 60.7% sensitivity for miR-506 analysis and 76.8% specificity and 75% specificity for miR-4316 analysis.
  
  
  CONCLUSIONS
  Our study revealed that elevated expression of miR-506 and miR-4316 in peripheral blood were potential molecular markers for early colorectal cancer.
authors.affiliation: Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland. krapa@poczta.onet.pl.
authors.forename: Paweł
authors.initials: P
authors.lastname: Krawczyk
authors.affiliation: Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Powrózek
authors.affiliation: Department of Oncological Gastroenterology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology in Warsaw, WK Roentgen 5, 02-781, Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Olesiński
authors.affiliation: Department of Oncological Gastroenterology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology in Warsaw, WK Roentgen 5, 02-781, Warsaw, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Dmitruk
authors.affiliation: Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Dziwota
authors.affiliation: Department of Lung and Chest Tumor, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology in Warsaw, WK Roentgen 5, 02-781, Warsaw, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kowalski
authors.affiliation: Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Milanowski
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012372:ROC Curve
chemical_list: C000626696:MIRN4316 microRNA, human; C561508:MIRN506 microRNA, human; D035683:MicroRNAs
keywords: Colorectal cancer; Early diagnosis and prevention; Liquid biopsy; microRNAs