title: High-resolution mapping of QTL for fatty acid composition in soybean using specific-locus amplified fragment sequencing.
abstract: We constructed a high-density linkage map comprising 3541 markers developed by specific-locus amplified fragment sequencing, and identified 26 stable QTL including nine novel loci, for fatty acid composition in soybean. Soybean oil quality and stability are mainly determined by the fatty acid composition of the seed. In the present study, we constructed a high-density genetic linkage map using 200 recombinant inbred lines derived from a cross between cultivated soybean varieties Luheidou2 and Nanhuizao, and SNP markers developed by specific-locus amplified fragment sequencing (SLAF-seq). This map comprises 3541 markers on 20 linkage groups and spans a genetic distance of 2534.42 cM, with an average distance of 0.72 cM between adjacent markers. Inclusive composite interval mapping revealed 26 stable QTL for five fatty acids, explaining 0.4-37.0% of the phenotypic variance for individual fatty acids across environments. Of these QTL, nine are novel loci (qLA1, qLNA2_1, qPA4_1, qLA4_1, qPA6_1, qSA12_1, qPA16_1, qOA18_1, and qFA19_1). These stable QTL harbor three fatty acid biosynthesis genes (GmFabG, GmACP, and GmFAD8), and 66 genes encoding lipid-related transcription factors. These stable QTL and tightly linked SNP markers can be used for marker-assisted selection in soybean breeding programs.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Shengxü
authors.initials: S
authors.lastname: Fan
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Fukuan
authors.initials: F
authors.lastname: Yu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Shengrui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Fenxia
authors.initials: F
authors.lastname: Han
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Shurong
authors.initials: S
authors.lastname: Yan
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China.
authors.forename: Lianzheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China. sunjunming@caas.cn.
authors.forename: Junming
authors.initials: J
authors.lastname: Sun
mesh_terms: D002874:Chromosome Mapping; D004843:Epistasis, Genetic; D005227:Fatty Acids / Q000737:chemistry*; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012639:Seeds / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods; D013025:Glycine max / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers