title: Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye.
abstract: Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Seon-Ju
authors.initials: SJ
authors.lastname: Yeo
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Bui Thi
authors.initials: BT
authors.lastname: Cuc
authors.affiliation: College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Soon-Ai
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Do Thi Hoang
authors.initials: DTH
authors.lastname: Kim
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Duong Tuan
authors.initials: DT
authors.lastname: Bao
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Trinh Thi Thuy
authors.initials: TTT
authors.lastname: Tien
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Nguyen Thi Viet
authors.initials: NTV
authors.lastname: Anh
authors.affiliation: Department of Pediatrics, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea.
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.affiliation: GenBody Inc, No.206, Biotech Business IC, DanKook University, San-29, Anseo-dong, Dongnam-gu, Cheonan, Republic of Korea.
authors.forename: Chom-Kyu
authors.initials: CK
authors.lastname: Chong
authors.affiliation: College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea. Electronic address: hankidad@wku.ac.kr.
authors.forename: Hak Sung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, 460, Iksan-daero, Iksan 54538, Republic of Korea. Electronic address: hyunpk@wku.ac.kr.
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002645:Chickens; D004196:Disease Outbreaks; D005456:Fluorescent Dyes; D006801:Humans; D057916:Influenza A Virus, H7N1 Subtype / Q000302:isolation & purification* / Q000472:pathogenicity; D009980:Influenza A virus / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes
keywords: Avian influenza A virus; Novel pyridine-styrylpyridine fluorescent dye; Photostability; Rapid fluorescent immunochromatographic test