title: Evaluation of Hematobin as a Vaccine Candidate to Control Haematobia irritans (Diptera: Muscidae) Loads in Cattle.
abstract: The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated. In this trial, horn fly loads and H. irritans's blood intake were assessed in vaccinated (n = 4) and control (n = 4) crossbred dark-coated steers, which were naturally infected. The vaccinated group received a 1 ml subcutaneous injection of 100 µg of HTB protein emulsified in 500 µl of Incomplete Freund Adjuvant (AIF) on days 0 and 30. The control group received on the same days 1 ml of distilled water emulsified in 500 µl of AIF. The vaccinated group had significantly more HTB-specific IgG antibodies after the HTB booster and had a lower fly load than the control group (206 ± 23 vs. 285 ± 23 flies per animal, respectively). Blood intake by H. irritans did not differ between groups. In summary, these results suggest that vaccinating cattle with HTB could reduce cattle H. irritans load.
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay ( mbreijo@fmed.edu.uy ; gabrielrocha85@gmail.com ; ximenaures@gmail.com ; palonzo@higiene.edu.uy ).|Corresponding author, e-mail: mbreijo@fmed.edu.uy.
authors.forename: M
authors.initials: M
authors.lastname: Breijo
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy).
authors.forename: S
authors.initials: S
authors.lastname: Rocha
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy).
authors.forename: X
authors.initials: X
authors.lastname: Ures
authors.affiliation: Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay (lpastro@fcien.edu.uy).
authors.forename: L
authors.initials: L
authors.lastname: Pastro
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy).
authors.forename: P
authors.initials: P
authors.lastname: Alonzo
authors.affiliation: Instituto de Higiene, Cátedra de Inmunología, Av. Alfredo Navarro 3051, 11600 Montevideo, Uruguay (cfernan@fq.edu.uy).
authors.forename: C
authors.initials: C
authors.lastname: Fernández
authors.affiliation: Laboratorio de Técnicas Nucleares, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay (meikleana@gmail.com).
authors.forename: A
authors.initials: A
authors.lastname: Meikle
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D004478:Ectoparasitic Infestations / Q000517:prevention & control / Q000662:veterinary*; D019476:Insect Proteins / Q000494:pharmacology*; D008297:Male; D009117:Muscidae / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014612:Vaccines
chemical_list: D019476:Insect Proteins; D011994:Recombinant Proteins; D014612:Vaccines
keywords: Haematobia irritans; cattle; hematobin; vaccine