title: Agrobacterium deltaense sp. nov., an endophytic bacteria isolated from nodule of Sesbania cannabina.
abstract: A Gram-negative, non-spore-forming, aerobic rods, strain YIC4121T, was isolated from root nodule of Sesbania cannabina grown in Dongying (Yellow River Delta), Shandong Province, PR China. Based on phylogenetic analysis of 16 S rRNA gene sequences, strain YIC4121Twas assigned to the genus Agrobacterium with 99.7, 99.3, 99.0, 98.8 and 98.7% sequence similarities to Agrobacterium radiobacter LMG140T, A. pusense NRCPB10T, A. arsenijevicii KFB 330T, A. nepotum 39/7Tand A. larrymoorei ATCC51759T. Analysis of the concatenated housekeeping genes (recA-atpD-glnII), showed lower sequence similarities (≤94.6%) between strain YIC4121Tand other recognized Agrobacterium species. Strain YIC4121Tshared whole-genome average nucleotide identities (ANI) 87.94, 91.27 and 77.42%, with A. pusense NRCPB10T, A. radiobacter LMG140Tand A. larrymoorei ATCC51759T. The predominant cellular fatty acids were C19:0cyclo ω8c, summed feature 2 (C12:0aldehyde/unknown 10.9525), summed feature 8 (C18:1ω7c/C18:1ω6c), C16:03 OH and C16:0. The G + C content of strain YIC4121Twas 59.80 mol%. Tween 80, lactulose, citric acid, α-ketoglutaric acid, glycyl-L-glutamic acid and 2, 3-butanediol could not be utilized as carbon source, distinguishing strain YIC4121Tfrom the other related species. Based on the distinctly genetic and phenotypic dissimilarity, a novel species Agrobacterium deltaense sp. nov. is proposed with YIC4121T(=HAMBI 3654T= LMG 29283T) as the type strain.
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Key Laboratory of Coastal Biology and Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China. xzhan@iga.ac.cn.
authors.forename: Xiao Zeng
authors.initials: XZ
authors.lastname: Han
authors.affiliation: State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.|Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Wen Feng
authors.initials: WF
authors.lastname: Chen
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China.
authors.forename: Wen Xiu
authors.initials: WX
authors.lastname: Zou
authors.affiliation: Key Laboratory of Coastal Biology and Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, People's Republic of China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
mesh_terms: D060054:Agrobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020043:Genes, Essential / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D049931:Sesbania / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: ANI; Agrobacterium; Sesbania cannabina