title: Genotypic and epitope characteristics of group A porcine rotavirus strains circulating in Canada.
abstract: Surveillance of Rotavirus A (RVA) infections in North America swine populations are limited and not performed over a significant time period to properly assess the diversity of RVA strains in swine. The VP7 (G) and VP4 (P) genes of 32 Canadian RVA strains, circulating between 2009 and 2015 were sequenced, identifying the G3P[13], G5P[7], G9P[7], G9[13], and G9[19] genotype combinations. The Canadian RVA strains were compared to the RVA strains present in the swine ProSystems RCE rotavirus vaccine. The comparison revealed multiple amino acid differences in the G and P antigenic epitopes, regardless of the G and P genotypes but specifically in the Canadian G3, P[13] and P[19] genotypes. Our study further contributes to the characterization of RVA's evolution and disease mitigation among swine, which may optimize target vaccine design, thereby minimizing RVA disease in this economically important animal population.
authors.affiliation: Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan.
authors.forename: Omer
authors.initials: O
authors.lastname: Naseer
authors.affiliation: Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Jarvis
authors.affiliation: Vaccines Clinical Research and Development, GlaxoSmithKline Vaccines, Cambridge, MA, United States.
authors.forename: Max
authors.initials: M
authors.lastname: Ciarlet
authors.affiliation: Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States. Electronic address: marth027@umn.edu.
authors.forename: Douglas G
authors.initials: DG
authors.lastname: Marthaler
mesh_terms: D000818:Animals; D002170:Canada / Q000453:epidemiology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D000939:Epitopes
keywords: Antigenic epitopes; Phylogenetic; Rotavirus A virus; Vaccine