title: MiRNA-27a promotes the proliferation and invasion of human gastric cancer MGC803 cells by targetingSFRP1via Wnt/β-catenin signaling pathway.
abstract: This study aims to elucidate the effects of microRNA-27a (miR-27a) on the proliferation and invasion of gastric cancer (GC) cells by targeting SFRP1 via Wnt/β-catenin signaling pathway. GC and normal adjacent tissues were collected from 273 GC patients. Human gastric cancer cell line (MGC803) and normal human gastric mucosal cell line (GES-1) were cultured. The miR-27a mRNA expression was analyzed using Quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemistry (IHC) test was used to detect miR-27a and SFRP1 protein expressions. After transfection, cells were divided into five groups: the negative control (NC) group, the miR-27a inhibitor group, the miR-27a mimics group, the miR-27a inhibitor +SFRP1siRNA group and the miR-27a mimics + SFRP1 overexpression group. Western blotting was conducted to test SFRP1 and Wnt/β-catenin protein expression. Analysis for the target gene of miR-27a was performed using Luciferase assay. Cell proliferation, migration and invasion were determined by CCK8 and Transwell assay. The dual-luciferase reporter assay system was applied to analyze the effects of miR-27a on Wnt/β-catenin signaling pathway. In GC tissue and cell line, miR-27a protein and mRNA expressions were up-regulated, and SFRP1 protein and mRNA expressions were down-regulated. Luciferase assay indicated that miR-27a might targetSFRP1and regulate its expressions. When miR-27a was down-regulated, SFRP1 was up-regulated, and β-catenin, Wnt, p-β-catenin, and p-Wnt were significantly down-regulated. Compared with the NC group, the proliferation, migration and invasion of GC cells were remarkably increased in the miR-27a group, but these were declined in the miR-27a mimics + SFRP1 overexpression group. The proliferation, migration and invasion of GC cells were elevated in the miR-27a inhibitor + SFRP1 siRNA group compared with the miR-27a inhibitor group. These results showed that miR-27a was highly expressed in GC tissues and cells, and it might promote cell proliferation, migration and invasion by targetingSFRP1via the activation of Wnt/β-catenin signaling pathway.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Radiation Oncology, Jiangxi Cancer Hospital Nanchang 330029, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
authors.forename: Ni
authors.initials: N
authors.lastname: Guo
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanchang University Nanchang 330006, P. R. China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Jiangxi Cancer Hospital Nanchang 330029, P. R. China.
authors.forename: Yu-Qian
authors.initials: YQ
authors.lastname: Liao
keywords: MGC803 cells; MicroRNA-27a; SFRP1; Wnt/β-catenin signaling pathway; gastric cancer; invasion; migration; proliferation