title: Efficacy of a new spot-on formulation of selamectin plus sarolaner for cats against adult Ctenocephalides felis, flea egg production and adult flea emergence.
abstract: A new spot-on formulation of selamectin plus sarolaner was evaluated against fleas for adulticidal efficacy, and for the effect on egg production and hatching when applied to flea-infested cats. Ten male and ten female adult domestic shorthair cats were randomly assigned to one of two treatment groups based on pre-treatment flea counts. Cats received topical treatment on Day 0 in a single spot to the dorsal scapular area with either a placebo formulation or with the combination formulation at the minimal dose of 6.0mg selamectin plus 1.0mg sarolaner per kg bodyweight. On Days -1, 5, 12, 19, 26 and 33, cats were infested with approximately 100 (±5) unfed Ctenocephalides felis fleas. At 24h after treatment or 48h after subsequent flea infestation, cats were housed for a 20-h period in a cage to allow collection of flea eggs. At the end of this period, flea eggs were collected from the cages and cats were combed to remove and count live fleas. Emerged viable larvae and emerged adult fleas were counted 3days and 35days, respectively, after egg collection. The new spot-on formulation of selamectin plus sarolaner provided 100% efficacy against adult fleas up to Day 36 following a single application. Fleas on placebo-treated cats produced large numbers of eggs throughout the study, with individual counts ranging from 110 to 1256 eggs. Following treatment, four flea eggs were collected from a single selamectin/sarolaner-treated cat on Day 29, but there were no eggs collected from any other selamectin/sarolaner-treated animal during the study. No larvae or adult fleas developed from these four eggs. From the eggs collected from the placebo-treated cats, the mean percentage of live larvae and adults that emerged ranged from 67.3% to 84.2% and from 50.7% to 81.8%, respectively. A single topical treatment with a new spot-on formulation of selamectin plus sarolaner at the minimum label dose thus controlled fleas on cats and was 100% effective in preventing flea reproduction for over one month after treatment.
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA. Electronic address: adriano.vatta@zoetis.com.
authors.forename: Adriano F
authors.initials: AF
authors.lastname: Vatta
authors.affiliation: BerTek, Inc., P.O. Box 606, Greenbrier, AR 72058, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Everett
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Holzmer
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Cherni
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Vickie L
authors.initials: VL
authors.lastname: King
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Rugg
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, Hoge Wei 10, B-1930 Zaventem, Belgium.
authors.forename: Thomas
authors.initials: T
authors.lastname: Geurden
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000977:Antiparasitic Agents / Q000008:administration & dosage / Q000494:pharmacology; D002371:Cat Diseases / Q000188:drug therapy*; D002415:Cats; D058251:Ctenocephalides / Q000187:drug effects*; D005260:Female; D058267:Flea Infestations / Q000188:drug therapy* / Q000662:veterinary; D007555:Isoxazoles / Q000008:administration & dosage* / Q000494:pharmacology*; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D011897:Random Allocation; D012098:Reproduction / Q000187:drug effects; D016896:Treatment Outcome; D015053:Zygote / Q000187:drug effects
chemical_list: D000977:Antiparasitic Agents; D007555:Isoxazoles; D007559:Ivermectin; C414354:selamectin
keywords: Cat; Ctenocephalides felis; Flea; Isoxazoline; Larvicide; Ovicide; Reproduction; Sarolaner; Selamectin; Topical