title: Reproductive stage physiological and transcriptional responses to salinity stress in reciprocal populations derived from tolerant (Horkuch) and susceptible (IR29) rice.
abstract: Global increase in salinity levels has made it imperative to identify novel sources of genetic variation for tolerance traits, especially in rice. The rice landrace Horkuch, endemic to the saline coastal area of Bangladesh, was used in this study as the source of tolerance in reciprocal crosses with the sensitive but high-yielding IR29 variety for discovering transcriptional variation associated with salt tolerance in the resulting populations. The cytoplasmic effect of the Horkuch background in leaves under stress showed functional enrichment for signal transduction, DNA-dependent regulation and transport activities. In roots the enrichment was for cell wall organization and macromolecule biosynthesis. In contrast, the cytoplasmic effect of IR29 showed upregulation of apoptosis and downregulation of phosphorylation across tissues relative to Horkuch. Differential gene expression in leaves of the sensitive population showed downregulation of GO processes like photosynthesis, ATP biosynthesis and ion transport. Roots of the tolerant plants conversely showed upregulation of GO terms like G-protein coupled receptor pathway, membrane potential and cation transport. Furthermore, genes involved in regulating membrane potentials were constitutively expressed only in the roots of tolerant individuals. Overall our work has developed genetic resources and elucidated the likely mechanisms associated with the tolerance response of the Horkuch genotype.
authors.affiliation: Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.|Department of Integrative Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
authors.forename: Samsad
authors.initials: S
authors.lastname: Razzaque
authors.affiliation: Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.|Department of Integrative Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
authors.forename: Taslima
authors.initials: T
authors.lastname: Haque
authors.affiliation: Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.|Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska 68583, USA.
authors.forename: Sabrina M
authors.initials: SM
authors.lastname: Elias
authors.affiliation: Plant Physiology Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh.
authors.forename: Md Sazzadur
authors.initials: MS
authors.lastname: Rahman
authors.affiliation: Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
authors.forename: Sudip
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Department of Integrative Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Schwartz
authors.affiliation: International Rice Research Institute, Los Banos, Laguna, Philippines.
authors.forename: Abdelbagi M
authors.initials: AM
authors.lastname: Ismail
authors.affiliation: Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska 68583, USA.
authors.forename: Harkamal
authors.initials: H
authors.lastname: Walia
authors.affiliation: Department of Integrative Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Juenger
authors.affiliation: Plant Biotechnology Lab, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
authors.forename: Zeba I
authors.initials: ZI
authors.lastname: Seraj
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D059372:Nucleotide Motifs / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger