title: Eosin fluorescence: A diagnostic tool for quantification of liver injury.
abstract: Hepatitis is one of the most common life threatening diseases. The diagnosis is mainly based on biochemical analysis such as liver function test. However, histopathological evaluation of liver serves far better for more accurate final diagnosis. The goal of our study was to evaluate the eosin fluorescence pattern in CCl4-induced liver injury model compared with normal and different treatment groups. For this purpose, liver tissues were stained with H/E and examined under bright field microscope but the fluorescence microscopy of H/E stained slides provided an interesting fluorescence pattern and was quite helpful in identifying different structures. Interesting fluorescence patterns were obtained with FITC, Texas Red and Dual channel filter cubes that were quite helpful in identifying different morphological features of the liver. During the course of hepatic injury, liver cells undergo necrosis, apoptosis and overall cellular microenvironment is altered due to the modification of proteins and other intracellular molecules. Intensified eosin fluorescence was observed around the central vein of injured liver compared to normal indicating enhanced binding of eosin to the more exposed amino acid residues. To conclude, eosin fluorescence pattern varies with the health status of a tissue and can be used further for the diagnosis and quantification of severity of various liver diseases.
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan; Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, 44000, Pakistan. Electronic address: hamidpcmd@yahoo.com.
authors.forename: Hamid
authors.initials: H
authors.lastname: Ali
authors.affiliation: Department of Physics and Applied Mathematics, PIEAS, Nilore, 45650, Islamabad, Pakistan; Department of Physics, Hazara University, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Safdar
authors.initials: S
authors.lastname: Ali
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mazhar
authors.affiliation: Faculty of Biological Sciences, Department of Biochemistry, Quaid-i-azam University, Islamabad, Pakistan.
authors.forename: Amjad
authors.initials: A
authors.lastname: Ali
authors.affiliation: Department of Zoology, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Azra
authors.initials: A
authors.lastname: Jahan
authors.affiliation: Laboratory of Germplasm Innovation and Molecular Breeding, Department of Vegetable Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Abid
authors.initials: A
authors.lastname: Ali
mesh_terms: D000818:Animals; D017209:Apoptosis; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology*; D008099:Liver / Q000473:pathology*; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004801:Eosine Yellowish-(YS)
keywords: Eosin fluorescence; H/E stain; In vivo; Liver injury; Molecular imaging; Multichannel fluorescence microscopy; Spectrum analysis