title: Study on the excision and integration mediated by class 1 integron in Enterococcus faecalis.
abstract: Recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. However, most of the investigations focused on Gram-positive bacteria with few exceptions. In this study, Enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. A total of eight plasmids were subjected to establish the transformants for excision and integration test. As results showed, positive excision assay was observed, which had been confirmed by the further integration assays and PCR amplification. The observation of class 1 integron mediated excision and integration of various exogenous antibiotics resistance genes should raise the attention of integrons as novel antibiotic resistance determinant in Gram-positive bacteria, especially in Enterococcus.
authors.affiliation: Clinical LaboratoryCenter, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: yuguangchao2017@163.com.
authors.forename: Guangchao
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Dermatology and Venerology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. Electronic address: yecongxiu1603@163.com.
authors.forename: Congxiu
authors.initials: C
authors.lastname: Ye
authors.affiliation: Clinical LaboratoryCenter, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: fuqiang1421@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Clinical LaboratoryCenter, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: liujuzhen8806@163.com.
authors.forename: Juzhen
authors.initials: J
authors.lastname: Liu
authors.affiliation: Clinical LaboratoryCenter, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: fanxiaoyi6203@163.com.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Fan
authors.affiliation: Clinical LaboratoryCenter, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: huangyunzu@163.com.
authors.forename: Yunzu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, 382 Zhonghuan Road East, Guangzhou 510006, China. Electronic address: zhoushishui2017@163.com.
authors.forename: Shishui
authors.initials: S
authors.lastname: Zhou
mesh_terms: D013293:Enterococcus faecalis / Q000235:genetics*; D005822:Genetic Vectors; D032023:Integrons; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014169:Transformation, Bacterial
keywords: Attsite; Enterococcusfaecalis; Excision; Gene cassette; Integration; Integron