title: The role of miR-320a and IL-1β in human chondrocyte degradation.
abstract: OBJECTIVE
  This study aimed to explore the role of miR-320a in the pathogenesis of osteoarthritis (OA).
  
  
  METHODS
  Human cartilage cells (C28/I2) were transfected with miR-320a or antisense oligonucleotides (ASO)-miR-320a, and treated with IL-1β. Subsequently the expression of collagen type II alpha 1 (Col2α1) and aggrecan (ACAN), and the concentrations of sulfated glycosaminoglycans (sGAG) and matrix metallopeptidase 13 (MMP-13), were assessed. Luciferase reporter assay, qRT-PCR, and Western blot were performed to explore whether pre-B-cell leukemia Homeobox 3 (PBX3) was a target of miR-320a. Furthermore, cells were co-transfected with miR-320a and PBX3 expressing vector, or cells were transfected with miR-320a and treated with a nuclear factor kappa B (NF-κB) antagonist MG132. The changes in Col2α1 and ACAN expression, and in sGAG and MMP-13 concentrations, were measured again. Statistical comparisons were made between two groups by using the two-tailed pairedt-test.
  
  
  RESULTS
  Expression of miR-320a was elevated in OA cartilage tissues and chondrocytes, and in IL-1β-stimulated C28/I2 cells (p < 0.05 or p < 0.01). MiR-320a overexpression enhanced IL-1β-induced down-regulation of Col2α1 and ACAN and sGAG, and increased the IL-1β-induced overexpression of MMP-13 (p < 0.01). PBX3 was a direct target of miR-320a. PBX3 and MG132 co-transfection attenuated the effects of miR-320a on the expression of Col2α1, ACAN, sGAG and MMP-13(p < 0.01).
  
  
  CONCLUSIONS
  Overexpression of miR-320a might enhance IL-1β-induced cartilage degradation factors. These effects might be via targeting PBX3 and regulating NF-κB.Cite this article:Y. Jin, X. Chen, Z. Y. Gao, K. Liu, Y. Hou, J. Zheng. The role of miR-320a and IL-1β in human chondrocyte degradation.Bone Joint Res2017;6:-203. DOI: 10.1302/2046-3758.64.BJR-2016-0224.R1.
authors.affiliation: Department of Orthopaedics, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou 450003, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Orthopaedics, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou 450003, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou 450003, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Gao
authors.affiliation: Department of Orthopaedics, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou 450003, China.
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou 450003, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Orthopaedics, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou 450003, China zhengjia1211@126.com.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
keywords: Extracellular matrix; NF-κB; Osteoarthritis (OA); PBX3; microRNA-320a