title: Comparison of Allogeneic and Syngeneic Rat Glioma Models by Using MRI and Histopathologic Evaluation.
abstract: Research in neurooncology traditionally requires appropriate in vivo animal models, on which therapeutic strategies are tested before human trials are designed and proceed. Several reproducible animal experimental models, in which human physiologic conditions can be mimicked, are available for studying glioblastoma multiforme. In an ideal rat model, the tumor is of glial origin, grows in predictable and reproducible patterns, closely resembles human gliomas histopathologically, and is weakly or nonimmunogenic. In the current study, we used MRI and histopathologic evaluation to compare the most widely used allogeneic rat glioma model, C6-Wistar, with the F98-Fischer syngeneic rat glioma model in terms of percentage tumor growth or regression and growth rate. In vivo MRI demonstrated considerable variation in tumor volume and frequency between the 2 rat models despite the same stereotactic implantation technique. Faster and more reproducible glioma growth occurred in the immunoresponsive environment of the F98-Fischer model, because the immune response is minimized toward syngeneic cells. The marked inability of the C6-Wistar allogeneic system to generate a reproducible model and the episodes of spontaneous tumor regression with this system may have been due to the increased humoral and cellular immune responses after tumor implantation.
authors.affiliation: Department of Veterinary Science, University of Turin, Grugliasco, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Biasibetti
authors.affiliation: Department of Veterinary Science, University of Turin, Grugliasco, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Valazza
authors.affiliation: Department of Veterinary Science, University of Turin, Grugliasco, Italy;, Email: mariateresa.capucchio@unito.it.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Capucchio
authors.affiliation: NeuroBioOncology Center, Polyclinic of Monza, Vercelli, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Annovazzi
authors.affiliation: Departments of Science and Pharmaceutical Technology , University of Turin, Turin, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Battaglia
authors.affiliation: Departments of Science and Pharmaceutical Technology , University of Turin, Turin, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Chirio
authors.affiliation: Departments of Science and Pharmaceutical Technology , University of Turin, Turin, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Gallarate
authors.affiliation: NeuroBioOncology Center, Polyclinic of Monza, Vercelli, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Mellai
authors.affiliation: Departments of Science and Pharmaceutical Technology , University of Turin, Turin, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Muntoni
authors.affiliation: Departments of Science and Pharmaceutical Technology , University of Turin, Turin, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Peira
authors.affiliation: Department of Oncology, University of Turin, Orbassano, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Riganti
authors.affiliation: NeuroBioOncology Center, Polyclinic of Monza, Vercelli, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Schiffer
authors.affiliation: Departments of Neuroscience, University of Turin, Turin, Italy.
authors.forename: Pierpaolo
authors.initials: P
authors.lastname: Panciani
authors.affiliation: Departments of Neuroscience, University of Turin, Turin, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Lanotte
mesh_terms: D064591:Allografts / Q000276:immunology / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D005910:Glioma / Q000276:immunology / Q000473:pathology*; D064596:Isografts / Q000276:immunology / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000662:veterinary; D051381:Rats / Q000235:genetics / Q000276:immunology*; D011916:Rats, Inbred F344 / Q000235:genetics / Q000276:immunology; D017208:Rats, Wistar / Q000235:genetics / Q000276:immunology; D015203:Reproducibility of Results