title: Measurements of Intra- and Extra-Cellular 5-Methyltetrahydrofolate Indicate thatBifidobacterium AdolescentisDSM 20083TandBifidobacterium PseudocatenulatumDSM 20438TDo Not Actively Excrete 5-MethyltetrahydrofolateIn vitro.
abstract: Certain intestinal bifidobacteria have the ability to synthesize folates.In vitroexperiments revealed a high production, cellular accumulation, and release of reduced folate vitamers like 5-methyltetrahydrofolate and tetrahydrofolate in folate-free medium (FFM). However, it is still unclear to which extent synthesized folates are polyglutamylated and probably not available for transport, and if they are actively released by excretion. To address these questions, we characterized intra- and extra-cellular pteroylmonoglutamates and polyglutamylated 5-methyltetrahydrofolate (5-CH3-H4PteGlu2-4) inBifidobacterium adolescentisDSM 20083TandBifidobacterium pseudocatenulatumDSM 20438Tin vitro. Folates were measured by means of stable isotope dilution assays (SIDA) coupled with LC-MS/MS analysis using [2H4]-5-methyltetrahydrofolic acid, [2H4]-tetrahydrofolic acid, and [2H4]-5-formyltetrahydrofolic acid as internal standards. Cell viability was examined by fluorescence microscopy. Quantitation of folate production byB. adolescentisduring the stationary phase revealed a linear increase of dead cells paralleled by increasing concentration of 5-formyltetrahydrofolate and 5-methyltetrahydrofolate (100% 5-CH3-H4PteGlu4) in FFM, whereas the intracellular concentrations of these vitamers remained constant. After 24 h,B. adolescentis(125 mg cells, wet weight) produced a total amount of 0.846 nmol 5-CH3-H4folate: 0.385 ± 0.059 nmol (46 ± 7%) and 0.461 ± 0.095 nmol (54 ± 11%) measured in the intracellular (viable cells; 52 ± 3% measured by fluorescence microscopy) and extracellular (lysed cells; 48 ± 3%) fraction, respectively. ForB. pseudocatenulatum(124 mg cells, wet weight), 1.135 nmol 5-CH3-H4folate was produced after 24 h, and a similar proportionality between intra- and extra-cellular folate concentrations and viable/lysed cells was observed. These results indicate that the strains tested produce and accumulate 5-CH3-H4PteGlu4for cellular metabolism, and that extracellular concentrations of the vitamer arise from cell lysis.
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität MünchenFreising, Germany; ZIEL Institute for Food and Health, Core Facility Microbiome/NGS, Technische Universiät MünchenFreising, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Kopp
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München Freising, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Dürr
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München Freising, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Steigleder
authors.affiliation: ZIEL Institute for Food and Health, Core Facility Microbiome/NGS, Technische Universiät MünchenFreising, Germany; Institute of Medical Microbiology, RWTH University HospitalAachen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clavel
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München Freising, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Rychlik
keywords: LC-MS/MS; bifidobacteria; folate bioavailability; folate synthesis; gut microbiota; stable isotope dilution assay