title: Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production from biodiesel by-product and propionic acid by mutant strains of Pandoraea sp.
abstract: Pandoraea sp. MA03 wild type strain was subjected to UV mutation to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was selected from 52 mutants affected in the propionate metabolism exhibiting a conversion rate of PA into 3HV units of 0.78 g g-1. The use of crude glycerol (CG) plus PA or valeric acid resulted in a copolymer with 3HV contents varying from 21.9 to 30 mol% and 22.2 to 36.7 mol%, respectively. Fed-batch fermentations were performed using CG and PA and reached a 3HV yield of 1.16 g g-1, which is 86% of the maximum theoretical yield. Nitrogen limitation was a key parameter for polymer accumulation reaching up to 63.7% content and 18.1 mol% of 3HV. Henceforth, mutant prp25 is revealed as an additional alternative to minimize costs and support the P(3HB-co-3HV) production from biodiesel by-products. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1077-1084, 2017.
authors.affiliation: Inst. de Biociências, Universidade Estadual Paulista, UNESP, Av.24A, 1515, Rio Claro, SP, 13506-900, Brazil.
authors.forename: Fabrício C
authors.identifier: http://orcid.org/0000-0001-8095-2156
authors.initials: FC
authors.lastname: de Paula
authors.affiliation: Inst. de Pesquisa em Bioenergia, Universidade Estadual Paulista, UNESP, Av.24A, 1515, Rio Claro, SP, 13506-900, Brazil.
authors.forename: Carolina B C
authors.initials: CBC
authors.lastname: de Paula
authors.affiliation: Inst. de Ciências Biomédicas, Universidade de São Paulo, USP, Av. Prof. Lineu Prestes, 1374, Butantã, São Paulo, SP, 05508-900, Brazil.
authors.forename: José Gregório C
authors.initials: JGC
authors.lastname: Gomez
authors.affiliation: Inst. für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Münster, Germany.|Faculty of Environmental Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinbüchel
authors.affiliation: Inst. de Biociências, Universidade Estadual Paulista, UNESP, Av.24A, 1515, Rio Claro, SP, 13506-900, Brazil.
authors.forename: Jonas
authors.initials: J
authors.lastname: Contiero
mesh_terms: D056804:Biofuels; D042521:Burkholderiaceae / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D011422:Propionates / Q000737:chemistry / Q000378:metabolism*; D014466:Ultraviolet Rays; D000094122:Polyhydroxybutyrates
chemical_list: D056804:Biofuels; D011091:Polyesters; D011422:Propionates; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C029658:propionic acid
keywords: Pandoraea sp; biodiesel; biopolymer; glycerol; polyhydroxyalkanoates