title: Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa.
abstract: Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem.Turnera diffusa(damiana) is a shrub that grows throughout Mexico and is traditionally used for many illnesses including diabetes. Although a large number of plant metabolites are known, there are no reports indicating which of these are responsible for this activity, and this identification was the objective of the present work. Through bioassay-guided fractionation of a methanolic extract obtained from the aerial part ofT. diffusa, teuhetenone A was isolated and identified as the main metabolite responsible for the plant's hypoglycemic activity. Alpha-glucosidase inhibitory activity and cytotoxicity of this metabolite were determined. Hypoglycemic and antidiabetic activities were evaluated in a murine model of diabetes in vivo, by monitoring glucose levels for six hours and comparing them with levels after administering various controls. Teuhetenone A was not cytotoxic at the tested concentrations, and did not show inhibitory activity in the glucosidase test, and the in vivo assays showed a gradual reduction in glucose levels in normoglycemic and diabetic mice. Considering these results, we suggest that teuhetenone A has potential as an antidiabetic compound, which could be further submitted to preclinical assays.
authors.affiliation: Departmento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, N.L., C.P. 64460, Mexico. ap._183@hotmail.com.
authors.forename: Aída
authors.initials: A
authors.lastname: Parra-Naranjo
authors.affiliation: Departmento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, N.L., C.P. 64460, Mexico. ceciliadmontemayor@gmail.com.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Delgado-Montemayor
authors.affiliation: Departmento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, N.L., C.P. 64460, Mexico. jany1524@hotmail.com.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Fraga-López
authors.affiliation: Depto. de Fisiología y Fisiopatología, Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Calle Leñeros s/n, Col. Los Volcanes, Cuernavaca Mor. C.P. 62350, Mexico. gabriela.castaneda@uaem.mx.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Castañeda-Corral
authors.affiliation: Departmento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, N.L., C.P. 64460, Mexico. salazar121212@yahoo.com.mx.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Salazar-Aranda
authors.affiliation: Depto. de Fisiología y Fisiopatología, Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Calle Leñeros s/n, Col. Los Volcanes, Cuernavaca Mor. C.P. 62350, Mexico. juan.acevedo@uaem.mx.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Acevedo-Fernández
authors.affiliation: Departmento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, N.L., C.P. 64460, Mexico. nwaksman@gmail.com.
authors.forename: Noemi
authors.initials: N
authors.lastname: Waksman
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D029647:Turnera / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000520:alpha-Glucosidases
keywords: Turnera diffusa; antidiabetic; damiana; diabetes mellitus; teuhetenone A