title: Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides.
abstract: The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disk degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disk material was bathed in 10 or 100μM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disk material among groups, but IL-1β and IL-6 expression was decreased in the fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disk degeneration.
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Ding
authors.affiliation: Department of Anesthesiology, University of Virginia, Charlottesville, VA, USA.
authors.forename: Azra
authors.initials: A
authors.lastname: Oklopcic
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA.
authors.forename: Bayan
authors.initials: B
authors.lastname: Aghdasi
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, University of Virginia, Charlottesville, VA, USA; Department of Anesthesiology, University of Colorado, Aurora, CO, USA. Electronic address: vesna.jevtovic-todorovic@ucdenver.edu.
authors.forename: Vesna
authors.initials: V
authors.lastname: Jevtovic-Todorovic
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA. Electronic address: xl2n@virginia.edu.
authors.forename: Xudong
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000187:drug effects; D058847:Inflammasomes / Q000378:metabolism*; D055959:Intervertebral Disc Degeneration / Q000150:complications* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009479:Neuropeptides / Q000378:metabolism*; D010146:Pain / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D011843:Radiculopathy / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009479:Neuropeptides; C492716:Nlrp3 protein, mouse
keywords: DRG; Fullerene; Hyperalgesia; Inflammasome; Low back pain; Radiculopathy