title: [How I explore…. HLA class II by ultrastructure in toxic epidermal necrolysis].
abstract: Toxic epidermal necrolysis (TEN, Lyell syndrome) is a severe paroxystic drug reaction whose inductive mechanisms remain poorly understood. The HLA glycoproteins are possibly involved in the disease process. Such investigations rely on biomolecular methods, and suggest specific interactions between some drugs or their metabolites and some HLA groups according to ethnicity of the TEN patients. Electron microscopy following the immunogold method for revealing HLA-DR did not disclose any evidence for distinguishing distinct patterns on Langerhans cell membrane between the initial and the resolution phases of TEN.
authors.affiliation: , Université de Franche-Comté, Besançon, France.
authors.forename: G E
authors.initials: GE
authors.lastname: Piérard
authors.affiliation: Département d'Hématologie et d'Oncologie, Hopital Universitaire, Cleveland, USA.
authors.forename: A
authors.initials: A
authors.lastname: Dowlati
authors.affiliation: Service de Dermatologie, CHR hutois, Huy, Belgique.
authors.forename: C
authors.initials: C
authors.lastname: Piérard-Franchimont
authors.affiliation: Laboratoire de Bioingénierie et d'Imagerie cutanée, Département des Sciences Cliniques, Université de Liège, Belgique.
authors.forename: P
authors.initials: P
authors.lastname: Paquet
authors.affiliation: Service de Dermatopathologie Unilab Lg, Liège, Belgique.
authors.forename: P
authors.initials: P
authors.lastname: Delvenne
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D013262:Stevens-Johnson Syndrome / Q000175:diagnosis*
keywords:  Drug eruption ;  Drug toxicity ;  HLA ;  Lyell syndrome ;  T lymphocyte ; Toxic epidermal necrolysis 