title: Acute Fasting Induces Expression of Acylglycerophosphate Acyltransferase (AGPAT) Enzymes in Murine Liver, Heart, and Brain.
abstract: During fasting, cells increase uptake of non-esterified fatty acids (NEFA) and esterify excess into phosphatidic acid (PtdOH), the common precursor of both triacylglycerols and phospholipids, using acylglycerophosphate acyltransferases/lysophosphatidic acid acyltransferases (AGPAT/LPAAT). Knowledge of the regulation of AGPAT enzymes is important for understanding fasting adaptations. Total RNA was isolated from liver, heart, and whole brain tissue of C57BL/6J mice fed ad libitum, or fasted for 16 h. Following fasting, induction of Agpat2, 3, 4, and 5 was observed in the liver, Agpat2 and 3 in heart tissue, and Agpat1, 2, and 3 in whole brain tissue. As a result, the relative abundance profile of the individual homologues within specific tissues was found to be significantly altered depending on the nutritive state of the animal. These data demonstrate tissue-specific effects of fasting on the regulation of different Agpat that are implicated in supporting unique downstream glycerolipid synthesis pathways.
authors.affiliation: Department of Kinesiology, Faculty of Applied Health Sciences, University of Waterloo, 200 University Avenue West, BMH1110, Waterloo, ON, N2L 3G1, Canada.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Bradley
authors.affiliation: Department of Kinesiology, Faculty of Applied Health Sciences, University of Waterloo, 200 University Avenue West, BMH1110, Waterloo, ON, N2L 3G1, Canada.
authors.forename: Emily B
authors.initials: EB
authors.lastname: Mardian
authors.affiliation: Department of Kinesiology, Faculty of Applied Health Sciences, University of Waterloo, 200 University Avenue West, BMH1110, Waterloo, ON, N2L 3G1, Canada.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Moes
authors.affiliation: Department of Kinesiology, Faculty of Applied Health Sciences, University of Waterloo, 200 University Avenue West, BMH1110, Waterloo, ON, N2L 3G1, Canada. reduncan@uwaterloo.ca.
authors.forename: Robin E
authors.initials: RE
authors.lastname: Duncan
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D005215:Fasting; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity; D014018:Tissue Distribution
chemical_list: D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase
keywords: Acylglycerophosphate acyltransferase (AGPAT)/lysophosphatidic acid acyltransferase (LPAAT); Fasting; Non-esterified fatty acids (NEFA); Phospholipid synthesis; Triacylglycerol (TAG)