title: Runt-Related Transcription Factor 2 (Runx2) Is Responsible for Galectin-3 Overexpression in Human Thyroid Carcinoma.
abstract: Runx2 promotes metastatic ability of cancer cells by directly activating some of the mediators regarding malignancy. Galectin-3 (Gal-3) extensively expressed in normal and transformed cells and it is responsible for many cellular processes. In this study, we aimed to investigate whether there is any relationship between runx2 transcription factor and regulation of galectin-3 expression in different human thyroid carcinoma cell lines. To show effects of runx2 transcription factor on gal-3 expression, we developed runx2 knockdown model in the thyroid carcinoma cell lines; anaplastic 8505C and 8305C and, papillary TPC-1 and follicular FTC-133 by using siRNA transfection. We analyzed the protein expressions and mRNA levels of gal-3 and MMP2/9 in the runx2-silenced cell lines using Western blotting, qPCR, and fluorescent microscopy. Our results showed that mRNA expression levels of gal-3 and MMP2/9 were downregulated in runx2-silenced cell lines. In this investigation, we revealed that regulation of gal-3 expression was strongly correlated with runx2 transcription factor in human thyroid carcinoma. Considering the contribution of human gal-3 in collaboration with MMP2/9 to the malignant characters of many cancers, regulation of their expressions through runx2 seems like one of the key regulatory mechanism for malignant potential of human thyroid carcinoma. Accordingly, runx2 transcription factor inhibitors can be a potential target in order to prevent gal-3 mediated malignancy of human thyroid carcinoma. J. Cell. Biochem. 118: 3911-3919, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Science, Department of Biology, Istanbul University, Vezneciler, Istanbul, 34134, Turkey.
authors.forename: Engin
authors.identifier: 0000-0003-0866-8796
authors.initials: E
authors.lastname: Kaptan
authors.affiliation: Faculty of Science, Department of Biology, Istanbul University, Vezneciler, Istanbul, 34134, Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Sancar Bas
authors.affiliation: Faculty of Science, Department of Biology, Istanbul University, Vezneciler, Istanbul, 34134, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Sancakli
authors.affiliation: Art and Science Faculty, Department of Biology, Harran University, Sanliurfa, Osmanbey, 63300, Turkey.
authors.forename: Hatice Gumushan
authors.initials: HG
authors.lastname: Aktas
authors.affiliation: Faculty of Engineering, Department of Chemistry, Istanbul University, Avcilar, Istanbul, 34320, Turkey.
authors.forename: Bertan Boran
authors.initials: BB
authors.lastname: Bayrak
authors.affiliation: Faculty of Engineering, Department of Chemistry, Istanbul University, Avcilar, Istanbul, 34320, Turkey.
authors.forename: Refiye
authors.initials: R
authors.lastname: Yanardag
authors.affiliation: Faculty of Science, Department of Biology, Istanbul University, Vezneciler, Istanbul, 34134, Turkey.
authors.forename: Sehnaz
authors.initials: S
authors.lastname: Bolkent
mesh_terms: D002291:Carcinoma, Papillary / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D037502:Galectin 3 / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D037502:Galectin 3; D009363:Neoplasm Proteins; C493709:RUNX2 protein, human
keywords: Galectin-3; MMP-2; MMP-9; Runx2; THYROID CARCINOMA