title: RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes.
abstract: The RNA recognition motif of Arabidopsis splicing factor SF1 affects the alternative splicing of FLOWERING LOCUS M pre-mRNA and a heat shock transcription factor HsfA2 pre-mRNA. Splicing factor 1 (SF1) plays a crucial role in 3' splice site recognition by binding directly to the intron branch point. Although plant SF1 proteins possess an RNA recognition motif (RRM) domain that is absent in its fungal and metazoan counterparts, the role of the RRM domain in SF1 function has not been characterized. Here, we show that the RRM domain differentially affects the full function of the Arabidopsis thaliana AtSF1 protein under different experimental conditions. For example, the deletion of RRM domain influences AtSF1-mediated control of flowering time, but not the abscisic acid sensitivity response during seed germination. The alternative splicing of FLOWERING LOCUS M (FLM) pre-mRNA is involved in flowering time control. We found that the RRM domain of AtSF1 protein alters the production of alternatively spliced FLM-β transcripts. We also found that the RRM domain affects the alternative splicing of a heat shock transcription factor HsfA2 pre-mRNA, thereby mediating the heat stress response. Taken together, our results suggest the importance of RRM domain for AtSF1-mediated alternative splicing of a subset of genes involved in the regulation of flowering and adaptation to heat stress.
authors.affiliation: Division of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
authors.forename: Keh Chien
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Division of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Jang
authors.affiliation: Center for Desert Agriculture, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
authors.forename: Soon-Kap
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
authors.forename: Hyo-Young
authors.initials: HY
authors.lastname: Park
authors.affiliation: Division of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
authors.forename: May Phyo
authors.initials: MP
authors.lastname: Thu
authors.affiliation: Department of Life Sciences, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea. jhwanlee90@jbnu.ac.kr.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea. jkkim@korea.ac.kr.
authors.forename: Jeong-Kook
authors.initials: JK
authors.lastname: Kim
mesh_terms: D017398:Alternative Splicing / Q000235:genetics / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D000076249:Heat Shock Transcription Factors / Q000235:genetics / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D000072417:Protein Domains / Q000235:genetics / Q000502:physiology; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D000072260:RNA Splicing Factors / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000595143:At5g51300 protein, Arabidopsis; C522476:FLM protein, Arabidopsis; C000620134:HSFA2 protein, Arabidopsis; D000076249:Heat Shock Transcription Factors; D026161:MADS Domain Proteins; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D014157:Transcription Factors
keywords: Alternative splicing; AtSF1; FLM; Flowering time; Heat stress; RRM domain