title: Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays.
abstract: Lactococcus lactis subsp. lactis NCDO 2118 was recently reported to alleviate colitis symptoms via its anti-inflammatory and immunomodulatory activities, which are exerted by exported proteins that are not produced by L. lactis subsp. lactis IL1403. Here, we used in vitro and in silico approaches to characterize the genomic structure, the safety aspects, and the immunomodulatory activity of this strain. Through comparative genomics, we identified genomic islands, phage regions, bile salt and acid stress resistance genes, bacteriocins, adhesion-related and antibiotic resistance genes, and genes encoding proteins that are putatively secreted, expressed in vitro and absent from IL1403. The high degree of similarity between all Lactococcus suggests that the Symbiotic Islands commonly shared by both NCDO 2118 and KF147 may be responsible for their close relationship and their adaptation to plants. The predicted bacteriocins may play an important role against the invasion of competing strains. The genes related to the acid and bile salt stresses may play important roles in gastrointestinal tract survival, whereas the adhesion proteins are important for persistence in the gut, culminating in the competitive exclusion of other bacteria. Finally, the five secreted and expressed proteins may be important targets for studies of new anti-inflammatory and immunomodulatory proteins. Altogether, the analyses performed here highlight the potential use of this strain as a target for the future development of probiotic foods.
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Letícia C
authors.identifier: http://orcid.org/0000-0002-2036-4456
authors.initials: LC
authors.lastname: Oliveira
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Tessália D L
authors.initials: TD
authors.lastname: Saraiva
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Wanderson M
authors.initials: WM
authors.lastname: Silva
authors.affiliation: Department of Preventive Veterinary Medicine, State University of Londrina, Londrina-PR, Brazil.
authors.forename: Ulisses P
authors.initials: UP
authors.lastname: Pereira
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Bruno C
authors.initials: BC
authors.lastname: Campos
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Leandro J
authors.initials: LJ
authors.lastname: Benevides
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Flávia S
authors.initials: FS
authors.lastname: Rocha
authors.affiliation: Official Laboratory of Fisheries Ministry-Veterinary School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Henrique C P
authors.initials: HC
authors.lastname: Figueiredo
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Laboratory of Cellular and Molecular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.|Department of Microbiology, Immunology and Parasitology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG, Brazil.
authors.forename: Siomar C
authors.initials: SC
authors.lastname: Soares
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics*; D019936:Probiotics; D013312:Stress, Physiological
chemical_list: D001647:Bile Acids and Salts