title: Immobilization of BSA on ionic liquid functionalized magnetic Fe3O4nanoparticles for use in surface imprinting strategy.
abstract: Combining template immobilization with surface imprinting technology is an effective strategy to overcome the difficulties associated with macromolecular template removal and to achieve high specific recognition ability. In this work, ionic liquid functionalized Fe3O4nanoparticles were prepared via a simple two-step modification process and were used as substrate to immobilize bovine serum albumin (BSA). The zeta potential study revealed immobilization of BSA on the nanoparticles through multiple interactions, and the immobilization capacity was about nine times higher compared with that of bare Fe3O4. Subsequently, dopamine was utilized as functional monomer to prepare BSA surface imprinted nanoparticles. Fourier transform infrared spectroscopy, thermo-gravimetric analysis and transmission electron microscopy verified the successful preparation of BSA imprinted nanoparticles with core-shell structure. The influence of imprinted layer thickness on recognition ability of imprinted nanoparticles was investigated, and the results suggested that 20nm was an optimum thickness to achieve the best recognition ability. The adsorption isotherm studies showed that the imprinted nanoparticles had a significantly higher adsorption capacity and stronger binding affinity than the non-imprinted ones. Furthermore, the selective as well as the competitive adsorption studies revealed higher selectivity and recognition ability of the imprinted nanoparticles for BSA. Therefore, the proposed strategy is an effective way to obtain protein imprinted polymers with high adsorption capacity and good recognition ability, thus would be beneficial for the further development and application of protein imprinting technology.
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education/Shandong Province, Qilu University of Technology, Jinan 250353, China. Electronic address: qianliwei@mail.nwpu.edu.cn.
authors.forename: Liwei
authors.initials: L
authors.lastname: Qian
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Jiexuan
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education/Shandong Province, Qilu University of Technology, Jinan 250353, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Hou
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education/Shandong Province, Qilu University of Technology, Jinan 250353, China.
authors.forename: Jinfan
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education/Shandong Province, Qilu University of Technology, Jinan 250353, China.
authors.forename: Yongwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lei
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Miaoxiu
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address: zhangsufeng@sust.edu.cn.
authors.forename: Sufeng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D005290:Ferric Compounds / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008280:Magnetics; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; D052578:Ionic Liquids; C000499:ferric oxide; D012710:Serum Albumin, Bovine
keywords: Ionic liquids modification; Magnetic nanoparticles; Protein immobilization; Protein imprinted polymers