title: A highly sensitive capillary electrophoresis immunoassay strategy based on dual-labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate-specific antigen.
abstract: An enzyme and antibody dual labeled gold nanoparticles enhancing chemiluminescence strategy was developed for highly sensitive CE immunoassay (IA) of prostate-specific antigen (PSA). In this work, gold nanoparticles were labeled with horseradish peroxidase and antiprostate specific antigen-antibody, and used as the marker (Ab*). After PSA (antigen, Ag) was added into the system, a noncompetitive immune reaction was happen between Ab*and Ag to form an immune complex (Ag-Ab*). Subsequently, the obtained Ag-Ab*and unreacted Ab*were separated by CE, and the chemiluminescence intensity of Ag-Ab*was used to estimate PSA concentration. The calibration curve showed a good linearity in the range of 0.25-10 ng/mL. Based on a S/N of 3, the detection limit for PAS was estimated to be 0.092 ng/mL. Proposed CE method was applied for PSA quantification in human serum samples from healthy volunteers and patients with prostate cancer. The obtained results demonstrated that the proposed CE method may serve as an alternative tool for clinical analysis of PSA.
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
authors.forename: Shuting
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
authors.forename: Shulin
authors.identifier: 0000-0002-2560-042X
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor; D006046:Gold; D017430:Prostate-Specific Antigen
keywords: Capillary electrophoresis; Chemiluminescence immunoassays; Gold nanoparticles; Prostate-specific antigen