title: Post-prandial systemic 8-isoprostane increases after consumption of moderate and high-fat meals in insufficiently active males.
abstract: A single high-fat meal (HFM) leads to an increase in triglycerides and oxidative stress. Oxidative stress can be assessed via 8-isoprostane generation, which is associated with the development of asthma and cardiovascular disease. No previous research has investigated whether airway and systemic 8-isoprostane increases postprandially in nonasthmatic participants according to the energy and fat content of a meal. Our purpose was to assess airway and systemic 8-isoprostane after a HFM and a true-to-life moderate-fat meal (MFM). We hypothesized that airway and systemic 8-isoprostane would increase after a HFM and a MFM, with the greatest increase in the HFM condition. Eight nonasthmatic men (25.8±6.9years) completed the HFM and MFM trials in a randomized crossover design. After a 10-hour fast, participants consumed either a HFM (71.13kJ/kg body mass, 60% fat, 23% CHO) or a MFM (35.56kJ/kg body mass, 30% fat, 52% CHO). Exhaled breath condensate to assess airway 8-isoprostane was collected at baseline and at 3 and 6hours postmeal. Venous blood samples were collected at baseline and hourly until 6hours postmeal to assess triglycerides, and every 3hours for systemic 8-isoprostane. Airway 8-isoprostane responses were not significant as a main effect of time (P=.072), between conditions (P=.365), or between time and condition (P=.319) postmeal. Systemic 8-isoprostane increased over time (P<.001), but not between conditions (P=.124) or between time and condition (P=.649) postmeal. Triglyceride incremental area under the curve was different in the HFM compared to the MFM condition (P=.013). After a HFM and a MFM, 8-isoprostane increases systemically; however, airway 8-isoprostane does not change.
authors.affiliation: Department of Kinesiology, Kansas State University, Manhattan, KS 66506, USA; Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA. Electronic address: stephkurti@ksu.edu.
authors.forename: S P
authors.initials: SP
authors.lastname: Kurti
authors.affiliation: Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA; Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA. Electronic address: same@ksu.edu.
authors.forename: S R
authors.initials: SR
authors.lastname: Emerson
authors.affiliation: Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA; Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA. Electronic address: sararose@ksu.edu.
authors.forename: S K
authors.initials: SK
authors.lastname: Rosenkranz
authors.affiliation: Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA; Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA. Electronic address: cteeman@fiu.edu.
authors.forename: C S
authors.initials: CS
authors.lastname: Teeman
authors.affiliation: Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA; Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA. Electronic address: eemerson@ksu.edu.
authors.forename: E M
authors.initials: EM
authors.lastname: Emerson
authors.affiliation: Physical Activity and Nutrition-Clinical Research Consortium, Kansas State University, Manhattan, KS 66506, USA; Department of Food, Nutrition, Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA. Electronic address: brooke1@ksu.edu.
authors.forename: B J
authors.initials: BJ
authors.lastname: Cull
authors.affiliation: Department of Kinesiology, Kansas State University, Manhattan, KS 66506, USA. Electronic address: smith424@ksu.edu.
authors.forename: J R
authors.initials: JR
authors.lastname: Smith
authors.affiliation: Department of Kinesiology, Kansas State University, Manhattan, KS 66506, USA. Electronic address: caharms@ksu.edu.
authors.forename: C A
authors.initials: CA
authors.lastname: Harms
mesh_terms: D000328:Adult; D019540:Area Under Curve; D002318:Cardiovascular Diseases / Q000209:etiology; D018592:Cross-Over Studies; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D006801:Humans; D008297:Male; D062407:Meals; D019518:Postprandial Period; D012137:Respiratory System / Q000378:metabolism; D012307:Risk Factors; D057185:Sedentary Behavior; D014280:Triglycerides / Q000097:blood*; D055815:Young Adult
chemical_list: D004041:Dietary Fats; D014280:Triglycerides; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost
keywords: Cardiovascular disease risk; Oxidative stress; Randomized crossover design; Triglycerides