title: Generalized aggressive periodontitis: microbiological composition and clinical parameters in non-surgical therapy.
abstract: The aim of this study was to determine the variations in periodontal parameters and microbiological composition in periodontal pockets at the baseline and 3 and 6 months post treatmentin patients with Generalized Aggressive Periodontitis(GAP) undergoing non surgical periodontal treatment combined with chlorhexidine and systemic antibiotics. Medical and dental history was taken from 10 subjects, average age 30.6±2.7 years, diagnosed with GAP. A non surgical periodontal treatment combined with 0.12% chlorhexidine, 875 mg amoxicillin and 500 mg metronidazole every 12 hours for ten days was conducted. At each visit, the following measurements wererecorded: bacterial plaque (BP), bleeding on probing (BOP), probing depth (PD), clinical attachment level (CAL), hypermobility, and furcation lesions, and a sample of subgingivalplaque was taken from the site of the deepest probing depth of each sextant to identify Porphyromonas gingivalis, Treponemadenticola, Tannerella forsythia, Prevotella intermedia and Aggregatibacter actinomycetemcomitans using molecular biology techniques. After 6 months, the Wilcoxon test showed an increase of 0.97 mm in CAL (p=0.0047) and 2.54 mm in PD(p=0.009). A healthy site was defined as having a PD <5 mm, negative BOP and no pathogenic bacteria detected at 6 months, indicating significant improvement (p=0.008), with OR (95%CI) =4.7 (1.102220.11).With the treatment protocol used in this study, 6 months after treatment, patients had an approximately 4- fold higher possibility of presenting PD <5 mm and periodontal pockets without periodontal pathogenic bacteria.
authors.affiliation: Periodontology B Department, School of Dentistry, National University of Cordoba, Argentina.|Biochemistry and Molecular Biology, School of Medical Science, National Universityof Cordoba, Argentina.
authors.forename: María M
authors.initials: MM
authors.lastname: Usin
authors.affiliation: Biochemistry and Molecular Biology, School of Medical Science, National Universityof Cordoba, Argentina.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Tabares
authors.affiliation: Periodontology B Department, School of Dentistry, National University of Cordoba, Argentina.
authors.forename: Julieta
authors.initials: J
authors.lastname: Menso
authors.affiliation: Independencia Foundation, Córdoba, National University of Cuyo, Mendoza, Argentina.
authors.forename: Estela R
authors.initials: ER
authors.lastname: de Albera
authors.affiliation: Biochemistry and Molecular Biology, School of Medical Science, National Universityof Cordoba, Argentina. asembaj@biomed.uncor.edu.
authors.forename: Adela
authors.initials: A
authors.lastname: Sembaj
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification*; D010520:Aggressive Periodontitis / Q000382:microbiology*; D000658:Amoxicillin / Q000627:therapeutic use*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003773:Dental Plaque; D005260:Female; D006801:Humans; D008297:Male; D008795:Metronidazole; D017622:Periodontal Attachment Loss; D010514:Periodontal Pocket; D016966:Porphyromonas gingivalis
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole; D000658:Amoxicillin
keywords: aggressive periodontitis; periodontal treatment