title: Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surfacein vitro.
abstract: Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05).
authors.affiliation: University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK; Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Sattam bin Abdulaziz University, AlKharj, Saudi Arabia.
authors.forename: N R
authors.initials: NR
authors.lastname: Alqhtani
authors.affiliation: University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Logan
authors.affiliation: University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: S
authors.initials: S
authors.lastname: Meghji
authors.affiliation: University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: R
authors.initials: R
authors.lastname: Leeson
authors.affiliation: University College London, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Brett
keywords: Bisphosphonates; Bone remodelling/regeneration; Human mesenchymal stem cells; Titanium