title: Developmental validation of a 6-dye STR kit with 27 loci.
abstract: The EX27 System is a 6-dye (i.e., FAM, HEX, TAMAR, ROX, VIG, and SIZ-500) kit that combines the 24 autosomal STR loci (i.e., D3S1358, D13S317, D7S820, D16S539, D1S1656, Penta E, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391, FGA, D22S1045, D2S441, SE33) with a Y-STR locus (DYS391), an Indel site (YIndel) and Amelogenin, which is designed to amplify DNA from extracts as well as direct amplification from substrates. The new system contains most of the widely used autosomal STR loci around the world and is sensitive to the male component in mixtures and is more accurate in gender determination owing to the DYS391 and YIndel.To demonstrate the suitability for personal identification and parentage testing, the system was validated for sensitivity of detection, species specificity, inhibitor tolerance, DNA mixtures, reproducibility study, and concordance studies based on the SWGDAM developmental validation guidelines. In view of the results, the EX27 System is a robust and reliable amplification kit which can be used for human identification testing.
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China.
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: AGCU ScienTch Incorporation, Wuxi, 214174, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China.
authors.forename: Quyi
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China. Liuchaogzf@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
mesh_terms: D053523:Amelogenin / Q000235:genetics; D000818:Animals; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000295:instrumentation*; D004586:Electrophoresis; D006801:Humans; D054643:INDEL Mutation; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D053523:Amelogenin
keywords: 6-dye multiplexing system; Forensic Science; Short tandem repeat (STR)