title: Proteomic characterization and schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena leucocephala wood under submerged fermentation.
abstract: In this study Schizophyllum commune ISTL04 was grown on Leucaena leucocephala wood (LLW) for secretome analysis and schizophyllan production. There is no report on extracellular protein profile and schizophyllan production on woody biomass by this fungus under submerged fermentation conditions. Leucaena leucocephala, a promising bioenergy crop having high holocellulose content was used as substrate without pretreatment. The maximum sugar, extracellular protein and exopolysaccharide (EPS) production during fermentation was found to be 8.53±0.07mgmL-1, 391±7.51mgL-1and 4.2±0.1gL-1or 0.21gg-1LLW on day 18 respectively. The secretome profile was dominated by glycoside hydrolases followed by carbohydrate esterase and other oxidative enzymes. EPS was further characterized by FTIR and GC-MS for functional group, monomer composition and linkage analysis and was identified as schizophyllan. The result indicated that LLW can be utilized as a low cost substrate for enzyme cocktail and schizophyllan production.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Singh
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Madan
authors.initials: M
authors.lastname: Kumar
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: isthakur@mail.jnu.ac.in.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D005285:Fermentation; D040901:Proteomics; D012567:Schizophyllum / Q000201:enzymology*; D012566:Sizofiran; D014934:Wood
chemical_list: D012566:Sizofiran
keywords: Leucaena leucocephala; Schizophyllan; Schizophyllum commune; Secretome; Submerged fermentation