title: Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells.
abstract: The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.
authors.affiliation: The Brown Foundation Institute of Molecular Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States.
authors.forename: John L
authors.initials: JL
authors.lastname: Mazzilli
authors.affiliation: The Brown Foundation Institute of Molecular Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States.
authors.forename: Aleksey Y
authors.initials: AY
authors.lastname: Domozhirov
authors.affiliation: The Brown Foundation Institute of Molecular Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States.
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Mueller-Ortiz
authors.affiliation: Department of Ophthalmology and Visual Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Garcia
authors.affiliation: The Brown Foundation Institute of Molecular Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States. Electronic address: rick.a.wetsel@uth.tmc.edu.
authors.forename: Rick A
authors.initials: RA
authors.lastname: Wetsel
authors.affiliation: The Brown Foundation Institute of Molecular Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Zsigmond
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002454:Cell Differentiation; D002460:Cell Line; D005260:Female; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D010587:Phagocytosis; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors