title: Elective embryo transfers on Day 6 reduce implantation compared with transfers on Day 5.
abstract: Is there a difference in pregnancy rates between embryos transferred electively on Day 5 and Day 6, respectively?
  
  
  
  The chance of pregnancy is significantly reduced (odds ratio (OR): 0.34; 95% CI 0.22-0.52) if transfer is performed on Day 6 compared with Day 5.
  
  
  
  Several studies report that Day 5 transfers have higher implantation rates (IRs) when compared with Day 6 transfers. These studies were based on non-elective Day 6 transfers, where transfers on Day 6 were performed with developmentally delayed embryos. Traditionally, difference in IRs has therefore been explained by an impaired embryo quality. An alternative explanation is that endometrial receptivity is higher on Day 5 compared with Day 6.
  
  
  
  The study was conducted as a retrospective cohort follow-up study on single blastocyst transfers from February 2011 until August 2015 in patients aged <38 years, with ≥eight oocytes retrieved and no diagnosis of endometriosis. Non-elective Day 6 transfers were excluded. Post hoc power-calculations (two-sided level of significance 0.05, power of 0.80) indicate that 91 embryos were needed in each group to detect a reduction in IR (primary outcome) from 40 to 20%.
  
  
  
  Day 5 or Day 6 transfers were implemented accordingly: from 2011 till 2013, transfers were performed on Day 6. If Day 6 was a Sunday, patients received Day 5 transfers. From 2013 onward, blastocysts were transferred on Day 5. If Day 5 was a Sunday, the transfer was delayed to Day 6. Univariable logistic regression analysis was performed to identify potential confounders. Factors with a P-value <0.1 were included in the multivariable logistic regression analysis.
  
  
  
  We included 334 single elective Day 5 and 268 elective Day 6 transfers. The unadjusted odds for implantation between Day 5 and Day 6 groups were 0.35 (95% CI 0.25-0.49). A univariable logistic regression analysis identified maternal age, BMI, cumulative FSH dose, number of cryopreserved embryos, score of inner cell mass and trophectoderm and day of transfer as predictors of clinical pregnancy. When adjusting for these variables in a multivariable logistic regression analysis, the implantation odds for Day 5 transfer remained significantly higher than Day 6 (OR 0.34; 95% CI 0.22-0.52).
  
  
  
  The study was conducted on good prognosis patients. The majority of Day 6 transfers were performed in the beginning of the study period. Day 5 transfers were generally performed in the end of the study period. This difference in time of recruitment may cause a minor variation in the data but a subanalysis indicates that this potential variation is negligible. Day 5 scores were higher in the Day 5 transfer group.
  
  
  
  Based on the findings in this study, transfers should be performed on Day 5. If Day 5 transfers are logistically impossible to perform, it is be preferable to cryopreserve the blastocyst and transfer in another cycle on Day 5, as Day 6 transfers should be avoided.
  
  
  
  None.
authors.affiliation: Department of Clinical Medicine, Aarhus University Hospital, Palle Juul Jensen Boulevard, 8200 Aarhus N, Denmark.
authors.forename: V
authors.initials: V
authors.lastname: Poulsen
authors.affiliation: Department of Clinical Medicine, Aarhus University Hospital, Palle Juul Jensen Boulevard, 8200 Aarhus N, Denmark.|Fertility Clinic, Aalborg University Hospital, 9000 Aalborg, Denmark.
authors.forename: H J
authors.initials: HJ
authors.lastname: Ingerslev
authors.affiliation: Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul Jensen Boulevard, 8200 Aarhus N, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Kirkegaard
mesh_terms: D000328:Adult; D001755:Blastocyst; D015331:Cohort Studies; D015925:Cryopreservation; D003718:Denmark / Q000453:epidemiology; D004477:Ectogenesis; D005191:Family Characteristics; D005260:Female; D005307:Fertilization in Vitro; D005500:Follow-Up Studies; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D007248:Infertility, Male; D016015:Logistic Models; D008297:Male; D008423:Maternal Age; D017410:Practice Guidelines as Topic; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D056826:Single Embryo Transfer; D013997:Time Factors
keywords: IVF; blastocyst; elective single embryo transfer; extended culture; implantation rate