title: Efficacy of vaccination and nisin Z treatments to eliminate intramammary Staphylococcus aureus infection in lactating cows.
abstract: This study evaluated the effect of a Staphylococcus aureus bacterin and nisin on bovine subclinical mastitis. A total of 75 Holstein subclinically mastitic cows were randomly allocated to three groups with 25 cows per group. In group I, an intramammary infusion of nisin Z at a dose of 2.50×106IU was carried out once daily for three days, and an autogenous S. aureus bacterin was inoculated into the supramammary lymph node one week before and one week after nisin treatment. In group II, nisin was administered in the same way as in group I, but no bacterin was inoculated. Group III received no treatment and served as a control. Milk was aseptically sampled from the affected quarters before and 2, 4, and 6 weeks after treatment, for bacteriological examination and analyses of N-acetyl-β-D-glucosaminidase (NAGase) activity, somatic cell count (SCC), and milk protein and fat contents. Results indicated that, compared to the nisin-treated group, nisin-bacterin treatment significantly reduced intramammary S. aureus infections, reduced the number of quarters with milk SCCs of more than 5×105cells/ml, and increased the protein and fat contents of the milk. Therefore, nisin-bacterin therapy is suggested when subclinical mastitis occurs in lactating cows.
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Guan
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Wu
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Su
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Song-Hua
authors.initials: SH
authors.lastname: Hu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D002417:Cattle; D002452:Cell Count; D004041:Dietary Fats / Q000032:analysis; D005260:Female; D007774:Lactation; D008414:Mastitis, Bovine / Q000276:immunology / Q000517:prevention & control*; D008892:Milk / Q000737:chemistry / Q000166:cytology / Q000382:microbiology; D008894:Milk Proteins / Q000032:analysis; D009561:Nisin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000276:immunology; D014611:Vaccination / Q000662:veterinary
chemical_list: D000900:Anti-Bacterial Agents; D004041:Dietary Fats; D008894:Milk Proteins; D013209:Staphylococcal Vaccines; D009561:Nisin; C071364:nisin Z
keywords: Subclinical mastitis; Intramammary infection; Staphylococcus aureus; Bacterin; Nisin