title: Production of bioactive hydrolysate using protease, β-glucosidase and α-amylase of Bacillus spp. isolated from kinema.
abstract: The aim of this study was the production of soybean bioactive hydrolysate using Bacillus spp. isolated from kinema. Totally 251 bacteria isolated from kinema samples, collected at different time period were screened for protease, β-glucosidase and α-amylase activities and further identified by ARDRA based grouping followed by analysis of 16S rRNA gene sequence similarity. The results showed that Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus licheniformis were the major Bacillus species. Twelve fermentative strains belonging to these groups and having high protease, α-amylase and β-glucosidase activity were used for solid state fermentation. The best strains for soybean fermentation that result in production of protein hydrolysates rich in polyphenols that have higher bioactivity were B. subtilis KN12C, B. amyloliquefaciens KN2G and B. licheniformis KN13C. Potential isolates can be applied for the production of soybean hydrolysates and can also find application in production of value added products from by-products of soybean processing industries.
authors.affiliation: Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong 737102, Sikkim, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Rai
authors.affiliation: Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong 737102, Sikkim, India.
authors.forename: Samurailatpam
authors.initials: S
authors.lastname: Sanjukta
authors.affiliation: Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong 737102, Sikkim, India.
authors.forename: Rounak
authors.initials: R
authors.lastname: Chourasia
authors.affiliation: Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong 737102, Sikkim, India.
authors.forename: Ishani
authors.initials: I
authors.lastname: Bhat
authors.affiliation: Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong 737102, Sikkim, India.
authors.forename: Pardeep K
authors.initials: PK
authors.lastname: Bhardwaj
authors.affiliation: Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong 737102, Sikkim, India; Institute of Bioresources and Sustainable Development, Imphal 795001, Manipur, India. Electronic address: dbsahoo@hotmail.com.
authors.forename: Dinabandhu
authors.initials: D
authors.lastname: Sahoo
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D005285:Fermentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D000516:alpha-Amylases / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D000516:alpha-Amylases; D001617:beta-Glucosidase
keywords: Fermentation; Peptides; Polyphenols; Protease; α-Amylase; β-Glucosidase