title: Adsorption of Oligo-DNA on Magnesium Aluminum-Layered Double-Hydroxide Nanoparticle Surfaces: Mechanistic Implication in Gene Delivery.
abstract: Magnesium aluminum-layered double-hydroxide nanoparticles (LDH NPs) are promising drug-delivery vehicles for gene therapy, particularly for siRNA interference; however, the interactions between oligo-DNA and LDH surfaces have not been adequately elucidated. Through a mechanistic study, oligo-DNA initially appears to rapidly bind strongly to the LDH outer surfaces through interactions with their phosphate backbones via ligand exchange with OH-on Mg2+centers and electrostatic forces with Al3+. These initial interactions might precede diffusion into interlayer spaces, and this knowledge can be used to design better gene therapy delivery systems.
authors.forename: Kori A
authors.initials: KA
authors.lastname: Andrea
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University , Hangzhou, Zhejiang 310018, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Carrier
authors.forename: Melanie
authors.initials: M
authors.lastname: Campbell
authors.affiliation: Department of Physics, Acadia University , Wolfville, Nova Scotia B4P 2R6, Canada.
authors.forename: Margaret
authors.initials: M
authors.lastname: Buhariwalla
authors.forename: MacKenzi
authors.initials: M
authors.lastname: Mutch
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: MacQuarrie
authors.affiliation: Department of Physics, Acadia University , Wolfville, Nova Scotia B4P 2R6, Canada.
authors.forename: Craig
authors.initials: C
authors.lastname: Bennett
authors.forename: Martin
authors.initials: M
authors.lastname: Mkandawire
authors.forename: Ken
authors.initials: K
authors.lastname: Oakes
authors.affiliation: College of Marine Life and Fisheries, Huaihai Institute of Technology , Lianyungang, Jiang Su 222005, People's Republic of China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Lu
authors.forename: Xu
authors.identifier: 0000-0002-8098-0410
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry*; D018014:Gene Transfer Techniques; D008276:Magnesium Hydroxide / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D009838:Oligodeoxyribonucleotides; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide