title: Alleviative effect of selenium on inflammatory damage caused by lead via inhibiting inflammatory factors and heat shock proteins in chicken testes.
abstract: The aim of this study was to investigate ameliorative effect of selenium (Se) on lead (Pb)-induced inflammatory damage in chicken testes. One hundred eighty 7-day-old male chickens were randomly assigned into the control group, the Se group, the Pb group, and the Pb/Se group. Lead acetate was added in drinking water (350 mg/L Pb). Sodium selenite was added in the standard commercial diet (1 mg/kg Se). On the 30th, 60th, and 90th days of the experiment, 15 chickens of each group were euthanized. Inductively coupled plasma mass spectrometry, hematoxylin and eosin staining, real-time quantitative PCR, and Western blot were used. The results indicated that excess Pb increased nitric oxide content; inducible nitric oxide synthase (iNOS) activity; nuclear factor-kappa B (NF-κB), tumor necrosis factor-α, cyclooxygenase-2, prostaglandin E synthases, and iNOS mRNA levels in a time-dependent manner; NF-κB, iNOS, heat shock protein (HSP) 60, HSP70, and HSP90 protein levels; and Pb concentration. Excess Pb decreased Se concentration and induced histological changes. Se-alleviated Pb caused all of the above changes. Se improved Pb-caused inflammatory damage by decreasing the expression of inflammatory factors and heat shock proteins in the chicken testes. Our results provided theoretical basis of an alleviative effect of Se on Pb-induced bird testis damage.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China. guxianhong@vip.sina.com.
authors.forename: Xianhong
authors.initials: X
authors.lastname: Gu
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People's Republic of China. tengxiaohua@neau.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Teng
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D011897:Random Allocation; D012643:Selenium / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D052247:Nitric Oxide Synthase Type II; D012643:Selenium
keywords: Bird testis; Heat shock protein; Inflammatory damage; Lead acetate; NF-κB; Sodium selenite