title: Assessing safety and protein interactions of surface-modified iron oxide nanoparticles for potential use in biomedical areas.
abstract: We have investigated the electrostatic interaction between bare iron oxide nanoparticles (IONPs) or low molecular weight chitosan coated iron oxide nanoparticles (LMWC-IONPs) and hen egg white lysozyme (HEWL) at different pH values using protein-nanoparticle reverse charge parity model. Physicochemical characterization of both IONPs and LMWC-IONPs were carried out using DLS, TEM, FE-SEM, XRD, TGA, XPS and VSM analysis. DLS, TEM and FE-SEM results indicated that both IONPs were monodispersed, with size ranging from 8 to 20nm. The coating of LMWC on IONPs was confirmed using zeta potential, TGA, XRD and XPS measurements. The cytotoxicity of both IONPs and LMWC-IONPs was studied in vitro in A549 human lung alveolar epithelial cells to assess their use in biomedical applications. Furthermore, the interactions between protein-nanoparticles were investigated by UV-visible, fluorescence and circular dichroism spectroscopic techniques. The present study suggests that water soluble LMWC surface modified IONPs are the promising nanomaterials. The safety and biocompatibility of these nanoparticles render them suitable for biomedical applications.
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, NP Marg, Matunga, Mumbai 400019, India.
authors.forename: Sathish
authors.initials: S
authors.lastname: Dyawanapelly
authors.affiliation: Division of Structural Biology, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Jehangir Merwanji Street, Parel, Mumbai 400012, India.
authors.forename: Dhanashree D
authors.initials: DD
authors.lastname: Jagtap
authors.affiliation: Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, NP Marg, Matunga, Mumbai 400019, India.
authors.forename: Prajakta
authors.initials: P
authors.lastname: Dandekar
authors.affiliation: UGC-DAE Consortium for Scientific Research, Trombay, Mumbai 400085, India. Electronic address: ghoshg@csr.res.in.
authors.forename: Goutam
authors.initials: G
authors.lastname: Ghosh
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga, Mumbai 400019, India. Electronic address: rd.jain@ictmumbai.edu.in.
authors.forename: Ratnesh
authors.initials: R
authors.lastname: Jain
mesh_terms: D000072283:A549 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D055672:Static Electricity
chemical_list: D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; C000499:ferric oxide; D048271:Chitosan; C421864:hen egg lysozyme; D009113:Muramidase
keywords: Hen egg white lysozyme; Iron oxide nanoparticle; Low molecular weight chitosan; Protein-nanoparticle electrostatics interaction; reverse charge parity model