title: Xenopus Vasa Homolog XVLG1 is Essential for Migration and Survival of Primordial Germ Cells.
abstract: Xenopus vasa-like gene 1 (XVLG1), a DEAD-Box Helicase 4 (DDX4) gene identified as a vertebrate vasa homologue, is required for the formation of primordial germ cells (PGCs). However, it remains to be clarified when and how XVLG1 functions in the formation of the germ cells. To gain a better understanding of the molecular mechanisms underlying XVLG1 during PGC development, we injected XVLG1 morpholino oligos into germ-plasm containing blastomeres of 32-cell stage of Xenopus embryos, and traced cell fates of the injected blastomere-derived PGCs. As a result of this procedure, migration of the PGCs was impaired and the number of PGCs derived from the blastomeres was significantly decreased. In addition, TUNEL staining in combination with in situ hybridization revealed that the loss of PGCs peaked at stage 27 was caused by apoptosis. This data strongly suggests an essential role for XVLG1 in migration and survival of the germ cells.
authors.affiliation: 1 Department of Biological Function, Faculty of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-0022, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Shimaoka
authors.affiliation: 1 Department of Biological Function, Faculty of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-0022, Japan.|2 Genetic Engineering Team, RIKEN Center for Life Science Technologies, Minatojimaminamimachi, Chuou-ku, Kobe 650-0047, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Mukumoto
authors.affiliation: 1 Department of Biological Function, Faculty of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-0022, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tanigawa
authors.affiliation: 1 Department of Biological Function, Faculty of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-0022, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Komiya
mesh_terms: D000818:Animals; D002465:Cell Movement; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D055785:Gene Knockdown Techniques; D005854:Germ Cells / Q000502:physiology*; D060172:Morpholinos; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014981:Xenopus / Q000378:metabolism*
chemical_list: D060172:Morpholinos; D012333:RNA, Messenger; D053487:DEAD-box RNA Helicases
keywords: DDX4; XVLG1; Xenopus; germ cells; germ plasm; knockdown; morpholino oligo; vasa