title: Transcriptional Profile for Detoxification Enzymes AeaGGT1 and AaeGGT2 From Aedes aegypti (Diptera: Culicidae) in Response to Larvicides.
abstract: Aedes aegypti (L.) is the vector responsible for transmitting dengue, chikungunya, yellow fever, and Zika viruses, as well as other pathogens. Microbial larvicides based on Bacillus thuringiensis Berliner israelensis (Bti) and Saccharopolyspora spinosa Mertz and Yao, such as VectoBac 12AS and Natular 2EC, have been shown to be effective in reducing larval populations of Ae. aegypti. We examined the gene expression of two detoxification enzymes, glucosyl and glucuronosyl transferases (AaeGGT1 and AaeGGT2), through developmental stages and a time course study in response to larvicide exposure using qualitative real-time polymerase chain reaction (qPCR). AaeGGT1 and AaeGGT2 gene expressions were differentially regulated during development of the immature stages. We also found that male adults had higher expression than female adults after controlling for age effects. AaeGGT1 and AaeGGT2 gene expression were both upregulated in response to VectoBac 12AS and Natular 2EC treatments with the maximum level of expression occurring 24 h after treatment applications. This information sheds light on larvicide-induced changes in the physiology of Ae. aegypti with implications for development of mosquito control strategies.
authors.affiliation: Florida Medical Entomology Laboratory, University of Florida, 200 9th St. South East, Vero Beach, FL 32962.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Florida Medical Entomology Laboratory, University of Florida, 200 9th St. South East, Vero Beach, FL 32962.
authors.forename: Barry W
authors.initials: BW
authors.lastname: Alto
authors.affiliation: Florida Medical Entomology Laboratory, University of Florida, 200 9th St. South East, Vero Beach, FL 32962.
authors.forename: Dagne
authors.initials: D
authors.lastname: Duguma
mesh_terms: D000330:Aedes / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008297:Male; D010063:Ovum / Q000187:drug effects / Q000254:growth & development; D011679:Pupa / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D016930:Saccharopolyspora / Q000737:chemistry
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D005964:Glucosyltransferases; D014453:Glucuronosyltransferase
keywords: Aedes aegypti; Natular 2EC; VectoBac 12AS; larvicide; metabolic detoxification