title: Comparative proteomics ofRhizopus delemarATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation.
abstract: The filamentous fungusRhizopus delemarnaturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation inR. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production inR. delemar, and propose that a substantial part of the fumarate accumulated inR. delemarduring nitrogen starvation results from the urea cycle due to amino acid catabolism.
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Dorett I
authors.initials: DI
authors.lastname: Odoni
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Tamayo-Ramos
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Jasper
authors.initials: J
authors.lastname: Sloothaak
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Ruben G A
authors.initials: RGA
authors.lastname: van Heck
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.|LifeGlimmer GmBH, Berlin, Germany.
authors.forename: Vitor A P
authors.initials: VAP
authors.lastname: Martins Dos Santos
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Leo H
authors.initials: LH
authors.lastname: de Graaff
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Maria
authors.initials: M
authors.lastname: Suarez-Diez
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schaap
keywords: Amino acid metabolism; Fumarate; Nitrogen metabolism; Proteomics; Rhizopus delemar; Transcriptomics