title: GNG11 (G-protein subunit γ 11) suppresses cell growth with induction of reactive oxygen species and abnormal nuclear morphology in human SUSM-1 cells.
abstract: Enforced expression of GNG11, G-protein subunit γ 11, induces cellular senescence in normal human diploid fibroblasts. We here examined the effect of the expression of GNG11 on the growth of immortalized human cell lines, and found that it suppressed the growth of SUSM-1 cells, but not of HeLa cells. We then compared these two cell lines to understand the molecular basis for the action of GNG11. We found that expression of GNG11 induced the generation of reactive oxygen species (ROS) and abnormal nuclear morphology in SUSM-1 cells but not in HeLa cells. Increased ROS generation by GNG11 would likely be caused by the down-regulation of the antioxidant enzymes in SUSM-1 cells. We also found that SUSM-1 cells, even under normal culture conditions, showed higher levels of ROS and higher incidence of abnormal nuclear morphology than HeLa cells, and that abnormal nuclear morphology was relevant to the increased ROS generation in SUSM-1 cells. Thus, SUSM-1 and HeLa cells showed differences in the regulation of ROS and nuclear morphology, which might account for their different responses to the expression of GNG11. Thus, SUSM-1 cells may provide a unique system to study the regulatory relationship between ROS generation, nuclear morphology, and G-protein signaling.
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takauji
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Ikuru
authors.initials: I
authors.lastname: Kudo
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Atsuki
authors.initials: A
authors.lastname: En
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Matsuo
authors.affiliation: b Department of Biochemistry, Primeasia University, 9 Banani C/A Banani, Dhaka 1213, Bangladesh.
authors.forename: Mohammad Nazir
authors.initials: MN
authors.lastname: Hossain
authors.affiliation: c Department of Maternal-Fetal Biology, National Center for Child Health and Development, Tokyo 157-8535, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakabayashi
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.|d Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama, Kanagawa 231-0048, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Miki
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.affiliation: a Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan.|d Ichiban Life Corporation, 1-1-7 Horai-cho, Naka-ku, Yokohama, Kanagawa 231-0048, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism* / Q000473:pathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D044388:GTP-Binding Protein gamma Subunits / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D044388:GTP-Binding Protein gamma Subunits; D017382:Reactive Oxygen Species
keywords: GNG11; cellular immortalization; cellular senescence; espèces réactives de l’oxygène; forme du noyau; immortalisation cellulaire; nuclear shape; reactive oxygen species; sénescence cellulaire