title: Bioresponsive Release System for Visual Fluorescence Detection of Carcinoembryonic Antigen from Mesoporous Silica Nanocontainers Mediated Optical Color on Quantum Dot-Enzyme-Impregnated Paper.
abstract: An all-in-one paper-based analytical device (PAD) was successfully developed for visual fluorescence detection of carcinoembryonic antigen (CEA) on CdTe/CdSe quantum dot (QD)-enzyme-impregnated paper by coupling with a bioresponsive controlled-release system from DNA-gated mesoporous silica nanocontainers (MSNs). The assay was carried out in a centrifuge tube by using glucose-loaded MSNs with a CEA aptamer and a QD-enzyme-paper attached on the lid. Initially, single-strand complementary DNA to a CEA aptamer was covalently conjugated to the aminated MSN, and then glucose (enzyme substrate) molecules were gated into the pore with the help of the aptamer. Glucose oxidase (GOD) and CdTe/CdSe QDs were coimmobilized on paper for the visual fluorescence signal output. Upon target CEA introduction in the detection cell, the analyte specifically reacted with the immobilized aptamer on the MSN to open the pore, thereby resulting in the glucose release. The released glucose was oxidized by the immobilized GOD on paper to produce gluconic acid and hydrogen peroxide, and the latter quenched the fluorescence of CdTe/CdSe QDs, which could be determined by the naked eye on a portable smartphone and a commercial fluorospectrometer. Under optimal conditions, the PAD-based sensing system enabled sensitive discrimination of target CEA against other biomarkers or proteins in a linear range of 0.05-20 ng mL-1with a limit of detection of 6.7 pg mL-1(ppt). In addition, our strategy displayed high specificity, good reproducibility, and acceptable accuracy for analyzing human serum specimens with a commercial human CEA ELISA kit. Importantly, this methodology offers promise for simple analysis of biological samples and is suitable for use in the mass production of miniaturized devices, thus opening new opportunities for protein diagnostics and biosecurity.
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University , Fuzhou 350108, People's Republic of China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University , Fuzhou 350108, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shu
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University , Fuzhou 350108, People's Republic of China.
authors.forename: Dianping
authors.identifier: 0000-0002-0134-3983
authors.initials: D
authors.lastname: Tang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000097:blood* / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D010209:Paper; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D000068997:Smartphone; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D005947:Glucose