title: Angiogenenic effects of BpLec, a C-type lectin isolated from Bothrops pauloensis snake venom.
abstract: The present work reports the effects of a C-type lectin (BpLec) isolated from Bothrops pauloensis snake venom upon in vitro and in vivo angiogenesis models. Initially, we noted that BpLec was not cytotoxic to endothelial cells (tEnd) in doses up to 40μg/mL, but lower doses (2.5μg/mL, 5μg/mL, 10μg/mL and 20μg/mL) reduced tEnd cells adhesion to some extracellular matrix proteins and inhibited the in vitro vessel formation in Matrigel assay stimulated by bFGF. β-galactosides (d-lactose, N-acetyl-d-galactosamine and d-galactose) at 400mM reversed the effect of BpLec on tEnd cells adhesion, whereas d-galactose (400mM) partially reversed BpLec property of inhibiting vessel formation by tEnd cells in Matrigel. In vivo assays showed that BpLec increased hemoglobin content and capillary vessels number in polyether-polyurethane sponge discs subcutaneously implanted into dorsal skin mice. Additionally, BpLec also reduced collagen deposition and did not induce a pro-inflammatory response, as demonstrated by the decreased the secretion of some inflammatory cytokines, whereas myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) activities were not altered by BpLec. Taken together, our results indicate that BpLec might represent an interesting angiogenesis and inflammatory modulator that could also be used for searching possible therapeutic targets involved in these processes.
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Letícia Eulalio
authors.initials: LE
authors.lastname: Castanheira
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Daiana Silva
authors.initials: DS
authors.lastname: Lopes
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Sarah Natalie Cirilo
authors.initials: SNC
authors.lastname: Gimenes
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Simone Ramos
authors.initials: SR
authors.lastname: Deconte
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Bruno Antônio
authors.initials: BA
authors.lastname: Ferreira
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Patricia Terra
authors.initials: PT
authors.lastname: Alves
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Luiz Ricardo Goulart
authors.initials: LRG
authors.lastname: Filho
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Tatiana Carla
authors.initials: TC
authors.lastname: Tomiosso
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Renata Santos
authors.initials: RS
authors.lastname: Rodrigues
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Kelly Aparecida Geraldo
authors.initials: KAG
authors.lastname: Yoneyama
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Fernanda de Assis
authors.initials: FA
authors.lastname: Araújo
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil. Electronic address: veridiana@ufu.br.
authors.forename: Veridiana de Melo
authors.initials: VM
authors.lastname: Rodrigues
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D017837:Bothrops; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003435:Crotalid Venoms / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D037181:Lectins, C-Type / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D003435:Crotalid Venoms; D037181:Lectins, C-Type; D009195:Peroxidase; D000118:Acetylglucosaminidase
keywords: Angiogenesis; Bothrops pauloensis; C-type lectin; Inflammation