title: Long-Term Kinetics of Immunologic Components and Neurological Deficits in Rats Following Repetitive Mild Traumatic Brain Injury.
abstract: BACKGROUND Despite growing awareness of repetitive mild traumatic brain injury (rmTBI), understanding of the involvement of long-term kinetics of immunologic components in the central and peripheral immune system took part remains incomplete. The present study aimed to provide a quantitative assay for certain immune system parameters in rmTBI rats. MATERIAL AND METHODS Neurological functions were assessed by modified Neurological Severity Score (mNSS) and Morris Water Maze (MWM), immunologic components from brain and peripheral blood were analyzed by flow cytometry (FCM), and concentrations of inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 were measure by enzyme-linked immunosorbent assay (ELISA). RESULTS Neurological functions of rmTBI rats were seriously impaired. In the brain, T cells were up-regulated and peaked at week 1. The percentage of CD4+ T cells decreased from week 1 to week 4, while CD8+ T cells notably decreased at week 1, then increased until week 4. The infiltration proportion of Treg cells was reduced at week 1 and peaked at week 2. CD86+/CD11b+ M1 peaked at week 4 and CD206+/CD11b+ M2 rose at week 1. IL-6/IL-10 showed a similar pattern, whose rise corresponded to the decrease in TNF-α at week 2 after rmTBI. FCM demonstrated peripheral immune dysfunction after rmTBI. CONCLUSIONS mNSS and MWM demonstrated neuronal deficits in rmTBI rats, and central and peripheral immune systems were implicated in the pathophysiological processes of rmTBI. Long-term immune response may play dual roles in injury and repair of rmTBI.
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Laboratory of Neuro-Trauma and Neurodegenerative Disorders, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China (mainland).|Key Laboratory of Injuries, Variation and Regeneration of Nervous System, Tianjin, China (mainland).|Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China (mainland).
authors.forename: Ruojing
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Laboratory of Neuro-Trauma and Neurodegenerative Disorders, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China (mainland).|Key Laboratory of Injuries, Variation and Regeneration of Nervous System, Tianjin, China (mainland).|Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China (mainland).
authors.forename: Huabin
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin Geriatrics Institute, Tianjin, China (mainland).
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Han
authors.affiliation: Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China (mainland).|Key Laboratory of Injuries, Variation and Regeneration of Nervous System, Tianjin, China (mainland).|Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China (mainland).|Department of Neurosurgery, Tianjin Neurological Institute General Hospital, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Xintong
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Laboratory of Neuro-Trauma and Neurodegenerative Disorders, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China (mainland).|Key Laboratory of Injuries, Variation and Regeneration of Nervous System, Tianjin, China (mainland).|Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China (mainland).
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.affiliation: Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China (mainland).|Key Laboratory of Injuries, Variation and Regeneration of Nervous System, Tianjin, China (mainland).|Laboratory of Neuro-Trauma, Tianjin Neurological Institute, Tianjin, China (mainland).
authors.forename: Fanglian
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin Geriatrics Institute, Tianjin, China (mainland).|Laboratory of Neuro-Trauma and Neurodegenerative Disorders, Tianjin Geriatrics Institute, Tianjin, China (mainland).
authors.forename: Ping
authors.initials: P
authors.lastname: Lei
mesh_terms: D000818:Animals; D001921:Brain / Q000276:immunology* / Q000473:pathology*; D001924:Brain Concussion / Q000276:immunology* / Q000473:pathology; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D016753:Interleukin-10 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10