title: [Preparation and identification of the polyclonal antibody against ATRX-C2193-2492].
abstract: Objective To prepare the polyclonal antibody against human alpha thalassemia/mental retardation syndrome X-linked (ATRX) C-terminal and study the distribution and expression of ATRX protein in human cervical cancer tissues. Methods The antiserum was obtained from the BALB/c mice immunized with6His-ATRX-C2193-2492protein and then purified by the saturated ammonium sulfate precipitation and affinity chromatography. The titer of anti-ATRX polyclonal antibody was determined by ELISA. Its specificity was identified by SDS-PAGE analysis and Western blotting. The expression and location of ATRX in human cervical tissues were analyzed by immunohistochemistry. Results The titer of the polyclonal antibody against6His-ATRX-C2193-2492protein was about 1:12 800. The antibody could recognize6His-ATRX-C2193-2492protein specifically. With the polyclonal antibody, the target protein was found mainly in the nucleus of para-carcinoma tissues, and it was also expressed in the nucleus of cervical cancer tissue cells, but the expression in the latter was obviously lower. Conclusion The polyclonal antibody against6His-ATRX-C2193-2492protein has been produced successfully and used to detect ATRX protein in human cervical cancer tissues.
authors.affiliation: Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang 421001, China.
authors.forename: Shuangyang
authors.initials: S
authors.lastname: Tang
authors.affiliation: Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang 421001, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang 421001, China.
authors.forename: Ranhui
authors.initials: R
authors.lastname: Li
authors.affiliation: Second Affiliated Hopsital, University of South China, Hengyang 421001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang 421001, China.
authors.forename: Lanhua
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang 421001, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shen
authors.affiliation: Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang 421001, China. *Corresponding author, E-mail: wanyy1991@aliyun.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D004265:DNA Helicases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D038901:X-Linked Intellectual Disability / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D000075924:X-linked Nuclear Protein; D017085:alpha-Thalassemia / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000906:Antibodies; D009687:Nuclear Proteins; D004265:DNA Helicases; C092332:ATRX protein, human; D000075924:X-linked Nuclear Protein