title: The use of serine protease from Yarrowia lipolytica yeast in the production of biopeptides from denatured egg white proteins.
abstract: Deriving non-conventional enzymes from cheaper sources than those used for commercially available enzymes may result in the production of hydrolysates with beneficial features, while drastically reducing the cost of hydrolysis. This is especially significant for enzymatic hydrolysis as a method of protein waste utilization. We have previously described the ability of non-commercial serine protease from Yarrowia lipolytica yeast to produce/release bioactive peptides from egg white protein by-products (EP). The enzymatic hydrolysis of EP was carried out for 24 h using the serine protease at an enzyme: substrate ratio of 1:30 (w/w). The obtained hydrolysate was characterized by protein degradation of 38% and also exhibited an antioxidant and cytokine-inducing activity. The isolation procedure (ultrafiltration and RP-HPLC) of bioactive peptides from the EP hydrolysate provided peptide fractions with significant antioxidant and ACE inhibitory activities. Three homogeneous and three heterogeneous peptide fractions were identified using MALDI-TOF/MS and the Mascot Search Results database. The peptides, mainly derived from ovalbumin, were composed of 2-19 amino-acid residues. We have thus demonstrated a novel ability of serine protease from Y. lipolytica to release biopeptides from an EP by-product.
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pokora
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Zambrowicz
authors.affiliation: Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zabłocka
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Dąbrowska
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Szołtysik
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Konrad
authors.initials: K
authors.lastname: Babij
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Eckert
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Trziszka
authors.affiliation: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Józefa
authors.initials: J
authors.lastname: Chrzanowska
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004527:Egg Proteins / Q000737:chemistry* / Q000187:drug effects; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry*; D059748:Proteolysis / Q000187:drug effects; D057057:Serine Proteases / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D025062:Yarrowia / Q000201:enzymology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D004527:Egg Proteins; D010455:Peptides; D057057:Serine Proteases
keywords: ACE-inhibitor; Yarrowia lipolytica; antioxidant; bioactive peptide; serine protease