title: T-Bet independent development of IFNγ secreting natural T helper 1 cell population in the absence of Itk.
abstract: Th1, Th2, Th9 and Th17 cells are conventional CD4+effector T cells identified as secretors of prototypical cytokines IFNγ, IL4, IL9, and IL-17A respectively. Recently, populations of natural Th17 and Th1 cells (nTh17 and nTh1) with innate-like phenotype have been identified in the thymus that are distinct from conventional Th17 and Th1 cells. The absence of the Tec family kinase Interleukin-2 inducible T cell kinase (Itk) results in T cell immunodeficiency in mice and humans. Here we show that Itk negatively regulates the development of nTh1 cells that express IFNγ in a Tbet independent manner, and whose expansion can be enhanced by IL4. Furthermore, we show that robust induction of IL4 responses during Trichinella spiralis infection enhance the presence of nTh1 cells. We conclude T cell receptor signaling via Itk controls the development of natural Th1 cells, which are expanded by the presence of IL4.
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Kannan
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Sonia
authors.initials: S
authors.lastname: Mohinta
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Weishan
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.|Baker Institute for Animal Health, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Koylass
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.|Baker Institute for Animal Health, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Appleton
authors.affiliation: Department of Microbiology &Immunology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Avery
authors.initials: A
authors.lastname: August
mesh_terms: D000818:Animals; D007371:Interferon-gamma / Q000276:immunology* / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology; D020825:T-Box Domain Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D060168:Thymocytes / Q000276:immunology / Q000378:metabolism; D017160:Trichinella spiralis / Q000276:immunology / Q000502:physiology; D014235:Trichinellosis / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D000098815:T-bet Transcription Factor
chemical_list: D011948:Receptors, Antigen, T-Cell; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase