title: Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro.
abstract: Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.
authors.affiliation: Department of Surgery, College of Medicine and Health, Lishui University/Lishui People's Hospital, Lishui, Zhejiang 323000, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Surgery, College of Medicine and Health, Lishui University/Lishui People's Hospital, Lishui, Zhejiang 323000, China.
authors.forename: Fuying
authors.initials: F
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm; C487931:mirnlet7 microRNA, human