title: Magnetic separate "turn-on" fluorescent biosensor for Bisphenol A based on magnetic oxidation graphene.
abstract: Bisphenol A (BPA) is commonly considered to cause a health hazard to wildlife and humans, acting as an exogenous estrogen. Herein, a magnetic separate "turn-on" fluorescent method for the detection of BPA was proposed based on fluorescence resonance energy transfer (FRET) between fluorescein-labeled BPA aptamer and magnetic oxidation graphene (MGO). At different concentrations of BPA, the fluorescence intensity of the sensing system was varied. The detection limit of 0.071ng/mL was obtained with the linear range of 0.2-10ng/mL. The biosensor exhibited excellent anti-interference ability and selectivity in actual water samples.
authors.affiliation: College of Environmental Science Engineering, Key Laboratory of Environmental Biology Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Liu-Yin
authors.initials: LY
authors.lastname: Hu
authors.affiliation: College of Environmental Science Engineering, Key Laboratory of Environmental Biology Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China. Electronic address: cgniu@hnu.edu.cn.
authors.forename: Cheng-Gang
authors.initials: CG
authors.lastname: Niu
authors.affiliation: College of Environmental Science Engineering, Key Laboratory of Environmental Biology Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Wang
authors.affiliation: South China Institute of Environmental Sciences, Ministry of Environmental Protection of PRC, Guangzhou 510655, China. Electronic address: huangdawei@scies.org.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Huang
authors.affiliation: College of Environmental Science Engineering, Key Laboratory of Environmental Biology Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Environmental Science Engineering, Key Laboratory of Environmental Biology Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Zeng
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008280:Magnetics; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D006108:Graphite; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aptamer; Bisphenol A; Magnetic oxidation graphene; Turn-on fluorescence