title: Analysis of chemical constituents in an herbal formula Jitong Ning Tablet.
abstract: Jitong Ning Tablet (JTNT), a traditional Chinese herbal formula, consists of Eucommia ulmodies oliv, Angelicae pubescentis radix, Aconiti radix cocta, Corydalis yanhusuo w.t. wang, Glycyrrhizae radix et rhizoma, Paeoniae radix rubra and Radix puerariae. It has been demonstrated to show protective effects on ankylosing spondylitis and anti-inflammatory effects. The chemical compositions of JTNT, playing a key role in quality control, remain unknown. In this study, an ultra-performance liquid chromatography combined with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) method in both positive and negative ion mode was established to investigate the chemical constituents of JTNT formula. In total, 162 compounds including flavonoids, triterpenoids, coumarins, alkaloids, phenylpropionic acids, lignans, terpenoids, and organic acids were detected, 152 of which were unambiguously or tentatively identified by comparing their retention times and accurate mass measurement with reference compounds and data in literatures. Our results would benefit quality control and chemical basis for JTNT.
authors.affiliation: State Key Laboratory of Natural Medicines, Clinical Metabolomics Center, China Pharmaceutical University, Nanjing 210009, China; Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China.
authors.forename: Di
authors.initials: D
authors.lastname: Gao
authors.affiliation: Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Wang
authors.affiliation: Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Huo
authors.affiliation: Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China.
authors.forename: Navaneethakrishnan
authors.initials: N
authors.lastname: Polachi
authors.affiliation: Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Natural Medicines, Clinical Metabolomics Center, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Tasly Academy, State Key Laboratory of Critical Technology in Innovative Chinese Medicine, TCM Research Center, Tianjin 300402, China. Electronic address: songzh@tasly.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Song
authors.affiliation: State Key Laboratory of Natural Medicines, Clinical Metabolomics Center, China Pharmaceutical University, Nanjing 210009, China. Electronic address: Qilw@cpu.edu.cn.
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal; D013058:Mass Spectrometry; D029596:Paeonia; D013607:Tablets
chemical_list: D004365:Drugs, Chinese Herbal; D013607:Tablets
keywords: Chemical compositions; Jitong Ning Tablet; Quality control; Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q–TOF–MS)