title: Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization.
abstract: OBJECTIVE
  This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.
  
  
  METHODS
  CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.
  
  
  RESULTS
  Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.
  
  
  CONCLUSIONS
  Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.
authors.affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd, Puzi City, Chiayi County, 61363, Taiwan.|Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan.|Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan.|Department of Ophthalmology, Changhua Christian Hospital, Yun Lin Branch, No. 375, Shichang S. Rd., Xiluo Township, Yunlin County, 64866, Taiwan.
authors.forename: Chien-Neng
authors.initials: CN
authors.lastname: Kuo
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No. 300 Syuefu Rd., Chiayi City, 60004, Taiwan.
authors.forename: Ching-Hsein
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Changhua Christian Hospital, No. 135, Nanxiao St., Changhua City, Changhua County, 50006, Taiwan.|School of Medicine, Chung-Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City, 40201, Taiwan.
authors.forename: San-Ni
authors.initials: SN
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd, Puzi City, Chiayi County, 61363, Taiwan.|Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan.|Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan.
authors.forename: Jou-Cheng
authors.initials: JC
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd, Puzi City, Chiayi County, 61363, Taiwan.|Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan.|Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan.
authors.forename: Li-Ju
authors.initials: LJ
authors.lastname: Lai
authors.affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd, Puzi City, Chiayi County, 61363, Taiwan.|Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan.|Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan.
authors.forename: Chien-Hsiung
authors.initials: CH
authors.lastname: Lai
authors.affiliation: Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan.|Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan.|Department of Dermatology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Hung
authors.affiliation: Department of Pharmacology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung City, 80708, Taiwan. chlee0818@gmail.com.
authors.forename: Chien-Hsing
authors.initials: CH
authors.lastname: Lee
authors.affiliation: School of Medical and Health Sciences, Fooyin University, No. 151, Jinxue Rd., Daliao Dist., Kaohsiung City, 83102, Taiwan. xx377@fy.edu.tw.
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Chen
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D015153:Blotting, Western; D003228:Conjunctiva / Q000187:drug effects / Q000378:metabolism; D016510:Corneal Neovascularization / Q000188:drug therapy* / Q000378:metabolism; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D006684:HLA-DR Antigens; D042461:Vascular Endothelial Growth Factor A; C569902:hexahydrocurcumin; D016222:Fibroblast Growth Factor 2; D003474:Curcumin
keywords: Angiogenesis; Corneal neovascularization; Hexahydrocurcumin; VEGF; bFGF