title: Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin.
abstract: Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2aas a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs.
authors.affiliation: 1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamada
authors.affiliation: 1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Mika K
authors.initials: MK
authors.lastname: Kaneko
authors.affiliation: 1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Takuro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: 3 Graduate School of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
authors.forename: Osamu
authors.initials: O
authors.lastname: Ichii
authors.affiliation: 3 Graduate School of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: 1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .|4 New Industry Creation Hatchery Center, Tohoku University , Sendai, Japan .
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000918:Antibody Specificity; D016466:CHO Cells; D003106:Colon / Q000033:anatomy & histology / Q000378:metabolism; D003412:Cricetulus; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D061905:Single-Domain Antibodies / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000098853:Podoplanin
chemical_list: D015100:3,3'-Diaminobenzidine; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D061905:Single-Domain Antibodies; C490441:Pdpn protein, mouse; D000098853:Podoplanin; C092025:Pdpn protein, rat
keywords: PMab-1; chimeric antibody; monoclonal antibody; podoplanin