title: A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts.
abstract: Human telomerase RNA (hTR), which is one component of telomerase, was deemed to be a biomarker to monitor tumor cells due to its different expression levels in tumor cells and normal somatic cells. Thus far, plentiful fluorescent probes have been designed to investigate nucleic acids. However, most of them are limited since they are time-consuming, require professional operators and even result in false positive signals in the cellular environment. Herein, we report a dual-colored ratiometric-fluorescent oligonucleotide probe to achieve the reliable detection of human telomerase RNA in cell extracts. The probe is constructed using a dual-labeled fluorescent oligonucleotide hybridized with target-complemented Dabcyl-labeled oligonucleotide. In the presence of the target, the dual-labeled fluorescent oligonucleotide translates into a hairpin structure, which leads to the generation of the fluorescence resonance energy transfer (FRET) phenomenon under UV excitation. Compared to conventional methods, this strategy could effectively avoid false positive signals, and it not only possesses the advantages of simplicity and high specificity but also has the merits of signal stability and distinguishable color variation. Moreover, the quantitative assay of hTR would have a far-reaching impact on the telomerase mechanism and even tumor diagnosis research.
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Dianhua
authors.initials: D
authors.lastname: Ning
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Changtian
authors.initials: C
authors.lastname: He
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Zhengjie
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: TingTing
authors.identifier: http://orcid.org/0000-0003-3193-8504
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. ttzhao@iim.ac.cn ryliu@iim.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Renyong
authors.initials: R
authors.lastname: Liu
mesh_terms: D002457:Cell Extracts / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D019098:Telomerase / Q000032:analysis*
chemical_list: D002457:Cell Extracts; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase