title: Rapid Microfluidic Double-Jump Mixing Device for Single-Molecule Spectroscopy.
abstract: We introduce a microfluidic double-jump mixing device for investigating rapid biomolecular kinetics with confocal single-molecule spectroscopy. This device enables nonequilibrium dynamics to be probed, e.g., transiently populated intermediates that are inaccessible with existing single-molecule approaches. We demonstrate the potential and reliability of the method on time scales from milliseconds to minutes by investigating the coupled folding and binding reaction of two intrinsically disordered proteins and the conformational changes occurring in a large cytolytic pore-forming toxin.
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Fabian
authors.initials: F
authors.lastname: Dingfelder
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Bengt
authors.initials: B
authors.lastname: Wunderlich
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Stephan
authors.initials: S
authors.lastname: Benke
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Franziska
authors.initials: F
authors.lastname: Zosel
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Niels
authors.initials: N
authors.lastname: Zijlstra
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nettels
authors.affiliation: Department of Biochemistry, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.|Department of Physics, University of Zurich , Winterthurerstrasse 190, 8057 Zurich, Switzerland.
authors.forename: Benjamin
authors.identifier: 0000-0002-5970-4251
authors.initials: B
authors.lastname: Schuler