title: A Phylogenetically Informed Comparison of GH1 Hydrolases between Arabidopsis and Rice Response to Stressors.
abstract: Glycoside hydrolases Family 1 (GH1) comprises enzymes that can hydrolyze β-O-glycosidic bond from a carbohydrate moiety. The plant GH1 hydrolases participate in a number of developmental processes and stress responses, including cell wall modification, plant hormone activation or deactivation and herbivore resistance. A large number of members has been observed in this family, suggesting their potential redundant functions in various biological processes. In this study, we have used 304 sequences of plant GH1 hydrolases to study the evolution of this gene family in plant lineage. Gene duplication was found to be a common phenomenon in this gene family. Although many members of GH1 hydrolases showed a high degree of similarity in Arabidopsis and rice, they showed substantial tissue specificity and differential responses to various stress treatments. This differential regulation implies each enzyme may play a distinct role in plants. Furthermore, some of salt-responsive Arabidopsis GH1 hydrolases were selected to test their genetic involvement in salt responses. The knockout mutants ofAtBGLU1andAtBGLU19were observed to be less-sensitive during NaCl treatment in comparison to the wild type seedlings, indicating their participation in salt stress response. In summary, Arabidopsis and rice GH1 glycoside hydrolases showed distinct features in their evolutionary path, transcriptional regulation and genetic functions.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural UniversityTaian, China; College of Life Sciences, Nantong UniversityNantong, China; Ministry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, Nantong UniversityNantong, China.
authors.forename: Yun-Ying
authors.initials: YY
authors.lastname: Cao
authors.affiliation: College of Chemistry, Central China Normal University Wuhan, China.
authors.forename: Jing-Fang
authors.initials: JF
authors.lastname: Yang
authors.affiliation: School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong Shatin, Hong Kong.
authors.forename: Tie-Yuan
authors.initials: TY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University Taian, China.
authors.forename: Zhen-Feng
authors.initials: ZF
authors.lastname: Su
authors.affiliation: School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong KongShatin, Hong Kong; Shenzhen Research Institute, The Chinese University of Hong KongShenzhen, China.
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Zhu
authors.affiliation: School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong KongShatin, Hong Kong; Shenzhen Research Institute, The Chinese University of Hong KongShenzhen, China.
authors.forename: Mo-Xian
authors.initials: MX
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University Taian, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Fan
authors.affiliation: School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong KongShatin, Hong Kong; Shenzhen Research Institute, The Chinese University of Hong KongShenzhen, China.
authors.forename: Neng-Hui
authors.initials: NH
authors.lastname: Ye
authors.affiliation: Jiangsu Entry-exit Inspection And Quarantine Bureau Nanjing, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Feng
authors.affiliation: College of Life Sciences, Nantong University Nantong, China.
authors.forename: Ling-Juan
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: College of Chemistry, Central China Normal University Wuhan, China.
authors.forename: Ge-Fei
authors.initials: GF
authors.lastname: Hao
authors.affiliation: School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong KongShatin, Hong Kong; Shenzhen Research Institute, The Chinese University of Hong KongShenzhen, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University Taian, China.
authors.forename: Ying-Gao
authors.initials: YG
authors.lastname: Liu
keywords: gene expression; multiple alignment; phylogenetic analysis; promoter; stress treatments; β-glucosidase