title: Trehalose metabolism genes ofAphelenchoides besseyi(Nematoda: Aphelenchoididae) in hypertonic osmotic pressure survival.
abstract: Some organisms can survive extreme desiccation caused by hypertonic osmotic pressure by entering a state of suspended animation known as osmobiosis. The free-living mycophagous nematodeAphelenchoides besseyican be induced to enter osmobiosis by soaking in osmolytes. It is assumed that sugars (in particular trehalose) are instrumental for survival under environmental stress. InA. besseyi, two putative trehalose-6-phosphate synthase genes (TPS) encoding enzymes catalyzing trehalose synthesis, and a putative trehalase gene (TRE) encoding enzymes that catalyze hydrolysis of trehalose were identified and then characterized based on their transcriptome. RT-qPCR analyses showed that each of these genes is expressed as mRNA whenA. besseyiis entering in, during and recovering from osmobiosis, but only for certain periods. The changes of TRE activity were consistent with the transcript level changes of the TRE gene, and the trehalose level declined at certain periods when the nematodes were in, as well as recovering from, osmobiosis; this suggested that the hydrolysis of threhalose is essential. The feeding method of RNA interference (RNAi) was used to temporarily knock down the expression of each of the TPS and TRE genes. No obviously different phenotype was observed from any of the genes silenced individually or simultaneously, but the survival under hypertonic osmotic pressure reduced significantly and the recovery was delayed. These results indicated that trehalose metabolism genes should play a role in osmobiosis regulation and function within a restricted time frame.
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, China fengwang@nefu.edu.cn danleili@nefu.edu.cn.
authors.forename: Danlei
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, China fengwang@nefu.edu.cn danleili@nefu.edu.cn.
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0002-5405-7884
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, China.
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, China.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Ling
keywords: Aphelenchoides besseyi; Osmobiosis; Trehalase; Trehalose; Trehalose-6-phosphate synthase