title: Screening and investigation Lactobacillius spp. to improve Secale cereale silage quality.
abstract: Silage is a high-moisture-content forage that is used to feed livestock. Using silage as feed is economically feasible and suitable for cattle management. Secale cereale is considered as a high-quality forage plant. After the heading stage the biomass of S. cereale increases by up to 30%; however, lignification in the cell wall causes low fermentation efficiency and coefficient digestibility, and it may also be contaminated by mycotoxin-producing fungi and can impair the quality of the silage. Therefore, the aim of this study was to screen beneficial Lactobacillius spp. for S. cereale silage to reduce fungi contamination and to increase fibrinolytic ability in the silage. Up to 100 days fermentation period and total 180 lactic acid bacteria were isolated and only a single isolate, R4-26, showed strong acidification ability and antifungal activities against mycotoxin-producing fungus. According to quantitative RT-PCR results, the mycotoxin-producing fungal contamination was significantly reduced in the S. cereale silage containing the R4-26 isolate. In enzymatic assays, only a single isolate, R48-27 demonstrated cellulase, xylanase, chitinase and esterase activities. In conclusion, the beneficial Lactobacillius spp. strains have the ability to reduce mycotoxigenic fungi contamination and increase fibrinolytic activity to improve quality of the S. cereale silage.
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: Hye Sun
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Central Area Crop Breeding Division, NICS, RDA, Suwon, Korea.
authors.forename: Ouk Kyu
authors.initials: OK
authors.lastname: Han
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: Sam Churl
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus) and Institute Agriculture & Life Science, Gyeongsang National University, Jinju, Korea.
authors.forename: Youn-Sig
authors.initials: YS
authors.lastname: Kwak
mesh_terms: D005285:Fermentation; D005506:Food Contamination / Q000517:prevention & control*; D063427:Food Quality; D005658:Fungi; D007778:Lactobacillus; D009183:Mycotoxins; D012434:Secale; D012820:Silage / Q000382:microbiology; D013997:Time Factors
chemical_list: D009183:Mycotoxins
keywords: S. cereale silage; fibrinolysis; lactic acid bacteria; mycotoxin