title: Long non-coding RNA MEG3 inhibits adipogenesis and promotes osteogenesis of human adipose-derived mesenchymal stem cells via miR-140-5p.
abstract: lncRNAs are an emerging class of regulators involved in multiple biological processes. MEG3, an lncRNA, acts as a tumor suppressor, has been reported to be linked with osteogenic differentiation of MSCs. However, limited knowledge is available concerning the roles of MEG3 in the multilineage differentiation of hASCs. The current study demonstrated that MEG3 was downregulated during adipogenesis and upregulated during osteogenesis of hASCs. Further functional analysis showed that knockdown of MEG3 promoted adipogenic differentiation, whereas inhibited osteogenic differentiation of hASCs. Mechanically, MEG3 may execute its role via regulating miR-140-5p. Moreover, miR-140-5p was upregulated during adipogenesis and downregulated during osteogenesis in hASCs, which was negatively correlated with MEG3. In conclusion, MEG3 participated in the balance of adipogenic and osteogenic differentiation of hASCs, and the mechanism may be through regulating miR-140-5p.
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors.forename: Chanyuan
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, 100081, China. jialingfei1984@sina.com.|Central Laboratory, Peking University School and Hospital of Stomatology, 22 Zhongguancun Avenue South, Haidian District, Beijing, 100081, China. jialingfei1984@sina.com.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of General Dentistry 2, Peking University School and Hospital of Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing, 100081, China. esther1234@bjmu.edu.cn.|Beijing Key Laboratory of Digital Stomatology, National Engineering Lab for Digital and Material Technology of Stomatology, Beijing, 100081, China. esther1234@bjmu.edu.cn.
authors.forename: Wenshu
authors.initials: W
authors.lastname: Ge
authors.affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.|Beijing Key Laboratory of Digital Stomatology, National Engineering Lab for Digital and Material Technology of Stomatology, Beijing, 100081, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010012:Osteogenesis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*
chemical_list: C498907:MEG3 non-coding RNA, human; D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D062085:RNA, Long Noncoding
keywords: Adipogenesis; Human adipose-derived stem cells; MEG3; Osteogenesis; lncRNA; miR-140-5p; miRNA