title: A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite.
abstract: In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, Kyiv, 01003, Ukraine.
authors.forename: O V
authors.initials: OV
authors.lastname: Soldatkina
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, Kyiv, 01003, Ukraine. alex_sold@yahoo.com.|Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, Kyiv, 03143, Ukraine. alex_sold@yahoo.com.
authors.forename: O O
authors.initials: OO
authors.lastname: Soldatkin
authors.affiliation: Micro and Nanotechnology Department, Middle East Technical University, 06531, Ankara, Turkey.
authors.forename: B Ozansoy
authors.initials: BO
authors.lastname: Kasap
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, Kyiv, 01003, Ukraine.
authors.forename: D Yu
authors.initials: DY
authors.lastname: Kucherenko
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, Kyiv, 03143, Ukraine.
authors.forename: I S
authors.initials: IS
authors.lastname: Kucherenko
authors.affiliation: Micro and Nanotechnology Department, Middle East Technical University, 06531, Ankara, Turkey.|Central Laboratory, Middle East Technical University, 06531, Ankara, Turkey.
authors.forename: B Akata
authors.initials: BA
authors.lastname: Kurc
authors.affiliation: Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, Kyiv, 01003, Ukraine.|Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, Kyiv, 03143, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Dzyadevych
keywords: Amperometry; Biosensor; Enzyme; Glutamate oxidase; Silicalite