title: Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation againstTrichuris murisinfection.
abstract: Despite the importance of the adjuvant in the immunization process, very few adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant serine/threonine phosphatase 2A from the nematodeAngiostrongylus costaricensis(rPP2A) was used for intranasal immunization in mice previously infected withTrichuris murisThe animal intranasal immunization with rPP2A-OVS showed a reduction of 99.01% in the number of the nematode eggs and 97.90% in adult. The immunohistochemical analysis of the intestinal sections showed that in immunized animals with lipopeptide the mucus was significantly higher than in the other experimental groups. Also, these animals presented significantly different chemokine, CCL20 and CCL11, levels. However, although the number and size of Tuft cells did not vary between groups, the intensity of fluorescence per cell was significant in the group immunized with the rPP2A-OVS. The results of the present study suggest that mice immunized with the lipopeptide are capable of activating a combined Th17/Th9 response. This strategy of immunization may be of great applicability not only in immunotherapy and immunoprophylaxis to control diseases caused by nematodes but also in pathologies necessitating action at the level of the Th9 response in the intestinal mucosa.
authors.affiliation: Instituto de Biotecnología, Grupo de Bioquímica y Parasitología Molecular, Departamento de Parasitología, Universidad de Granada, Campus Universitario Fuentenueva, 18071 Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Gomez-Samblas
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Plaza de Ramón y Cajal s/n. Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: García-Rodríguez
authors.affiliation: Àrea de Parasitologia, Departament de Farmàcia i Tecnologia Farmacèutica i Parasitologia, Universitat de València, Av. V.A. Estellés, s/n, 46100 Burjassot (Valencia), Spain.|Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics, Health Research Institute-La Fe, Universitat de Valencia, Av. Fdo. Abril Martorell, 106, 46026 Valencia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Trelis
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Universitat de València, C/ Dr Moliner, 50, 46100 Burjassot (Valencia), Spain.
authors.forename: D
authors.initials: D
authors.lastname: Bernal
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Lopez-Jaramillo
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Santoyo-Gonzalez
authors.affiliation: Instituto de Biotecnología, Grupo de Bioquímica y Parasitología Molecular, Departamento de Bioquímica, Universidad de Granada, Campus Universitario Fuentenueva, 18071 Granada, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Vilchez
authors.affiliation: Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, University of Puerto Rico, School of Medicine. PO Box 365067, San Juan 00936-5067, Puerto Rico.
authors.forename: A M
authors.initials: AM
authors.lastname: Espino
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Plaza de Ramón y Cajal s/n. Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Bolás-Fernández
authors.affiliation: Instituto de Biotecnología, Grupo de Bioquímica y Parasitología Molecular, Departamento de Parasitología, Universidad de Granada, Campus Universitario Fuentenueva, 18071 Granada, Spain aosuna@ugr.es.
authors.forename: A
authors.identifier: 0000-0002-3818-1471
authors.initials: A
authors.lastname: Osuna
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000138:chemical synthesis; D000281:Administration, Intranasal; D000595:Amino Acid Sequence; D000818:Animals; D054413:Chemokine CCL11 / Q000235:genetics / Q000276:immunology; D054418:Chemokine CCL20 / Q000235:genetics / Q000276:immunology; D005260:Female; D015870:Gene Expression; D015801:Helminth Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology / Q000469:parasitology; D055666:Lipopeptides / Q000008:administration & dosage* / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D010270:Parasite Egg Count; D054648:Protein Phosphatase 2 / Q000008:administration & dosage* / Q000096:biosynthesis / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology; D016415:Sequence Alignment; D013450:Sulfones / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology / Q000469:parasitology; D014257:Trichuriasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D014258:Trichuris / Q000187:drug effects / Q000276:immunology; D018074:Vaccines, Conjugate / Q000008:administration & dosage*
chemical_list: D000276:Adjuvants, Immunologic; C493447:CCL20 protein, mouse; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D054418:Chemokine CCL20; D015801:Helminth Proteins; D007378:Interleukins; D055666:Lipopeptides; D011994:Recombinant Proteins; D013450:Sulfones; D018074:Vaccines, Conjugate; C009873:divinyl sulfone; D054648:Protein Phosphatase 2
keywords: Trichuris muris vaccination; chemokines; cytokines; lipid vinyl sulfone; self-assembling lipopeptide rPP2A