title: CAMSAP3 accumulates in the pericentrosomal area and accompanies microtubule release from the centrosome via katanin.
abstract: The epithelium has an apico-basal axis polarity that plays an important role in absorption, excretion and other physiological functions. In epithelial cells, a substantial number of non-centrosomal microtubules (MTs) are scattered in the cytoplasm with an apico-basal polarity and reorientate as epithelial cells perform different functions. Several previous studies have found that non-centrosomal MTs are nucleated at the centrosome, and then released and translocated elsewhere. However, the detailed process and molecular mechanism remain largely unknown. In this study, we found that Nezha, also called calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), a non-centrosomal MT minus-end protein, accumulates in the pericentrosomal area and accompanies the release of MTs from the centrosome; whereas depletion of CAMSAP3 prevented MT release and instead caused focusing of MTs at centrosomes. Further studies demonstrated that CAMSAP3 precisely coordinates with dynein and katanin to regulate the MT detachment process. In conclusion, our results indicate that CAMSAP3 is a key molecule for generation of non-centrosomal MTs.
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: RIKEN Center for Developmental Biology, 2-2-3 Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: RIKEN Center for Developmental Biology, 2-2-3 Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Takeichi
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China wxmeng@genetics.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Wenxiang
authors.identifier: 0000-0003-0060-0535
authors.initials: W
authors.lastname: Meng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D018385:Centrosome / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D000074201:Katanin / Q000378:metabolism*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: C578015:Camsap3 protein, mouse; D008869:Microtubule-Associated Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D004398:Dyneins; D000074201:Katanin
keywords: CAMSAP3; Centrosome; Katanin; Microtubules; Release