title: Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected withHelicobacter pylori.
abstract: BACKGROUND
  Helicobacter pylori(H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, andH. pyloriinfection in pediatric and adolescent patients.
  
  
  METHODS
  DNA samples were obtained from 285 gastric biopsies from pediatric patients.H. pyloriwas detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T>A) polymorphism of IL-8.
  
  
  RESULTS
  The histological analysis revealed the presence of gastritis in 158 patients (55.44%).H. pyloriwas found in 71 samples (24.9%). The -251 (T>A) polymorphism revealed that 58 (29.47%) samples were TT, 143 (50.18%) samples were TA, and 84 (20.35%) samples were AA.
  
  
  CONCLUSIONS
  Our findings suggest that IL8-251 A allele may be an important risk factor for the development of gastric disease when associated withH. pyloriinfection.
authors.affiliation: Faculdade de Medicina de Marília (FAMEMA), rua Lourival Freire, 240, Jardim Fragata, CEP 17519-050 Marília, SP Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Saes
authors.affiliation: Faculdade de Medicina de Marília (FAMEMA), rua Lourival Freire, 240, Jardim Fragata, CEP 17519-050 Marília, SP Brazil.
authors.forename: Roger Willian
authors.initials: RW
authors.lastname: de Labio
authors.affiliation: Universidade do Sagrado Coração, Bauru, SP Brazil.
authors.forename: Lucas Trevizani
authors.initials: LT
authors.lastname: Rasmussen
authors.affiliation: Faculdade de Medicina de Marília (FAMEMA), rua Lourival Freire, 240, Jardim Fragata, CEP 17519-050 Marília, SP Brazil.|Universidade do Sagrado Coração, Bauru, SP Brazil.
authors.forename: Spencer Luiz Marques
authors.initials: SLM
authors.lastname: Payão
keywords: -251 (T>A); Gastric disease; Helicobacter pylori; Interleukin 8; Polymorphism