title: Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines.
abstract: Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.
authors.affiliation: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, Jílové U Prahy, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Mucksová
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Plachý
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Staněk
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Hejnar
authors.affiliation: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, Jílové U Prahy, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Kalina
authors.affiliation: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, Jílové U Prahy, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Benešová
authors.affiliation: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, Jílové U Prahy, Czech Republic. pavel.trefil@bri.cz.
authors.forename: Pavel
authors.identifier: 0000-0003-1074-636X
authors.initials: P
authors.lastname: Trefil
mesh_terms: D000818:Animals; D020296:Animals, Congenic / Q000276:immunology / Q000821:virology; D018033:Antibodies, Bispecific / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002645:Chickens / Q000276:immunology / Q000821:virology*; D016207:Cytokines / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D007111:Immunity, Cellular / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology; D015778:Minor Histocompatibility Antigens / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology; D052736:Rous sarcoma virus / Q000276:immunology*; D001357:Sarcoma, Avian / Q000276:immunology / Q000517:prevention & control*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D018033:Antibodies, Bispecific; D015703:Antigens, CD; D000956:Antigens, Viral; D016207:Cytokines; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface; D014765:Viral Vaccines