title: Current smokers with hyperlipidemia lack elevated preβ1-high-density lipoprotein concentrations.
abstract: Preβ1-high-density lipoprotein (HDL) is an efficient acceptor of cell-derived free cholesterol, which is converted into lipid-rich HDL by lecithin-cholesterol acyltransferase. Previous studies have shown that preβ1-HDL is significantly higher in individuals with hyperlipidemia. Preβ1-HDL concentrations may be altered in smokers, who are at high risk for atherosclerosis.
  
  
  
  The aim of the present study was to investigate the effect of smoking on preβ1-HDL concentrations.
  
  
  
  We measured the preβ1-HDL concentration and lecithin-cholesterol acyltransferase-dependent conversion rate (CHTpreβ1) in 74 men (39 nonsmokers and 35 smokers) using an immunoassay.
  
  
  
  The smoker and nonsmoker groups were further divided into normolipidemic and hyperlipidemic subjects. Among nonsmokers, the mean preβ1-HDL concentration was 27% higher in hyperlipidemics than in normolipidemics (25.5 ± 6.7 vs 20.3 ± 4.6 mg/L apoAI, P < .01). In contrast, mean preβ1-HDL concentrations did not differ between hyperlipidemic and normolipidemic smokers (19.9 ± 3.1 vs 22.4 ± 6.9 mg/L apoAI). We found a positive correlation between preβ1-HDL concentration and CHTpreβ1in nonsmokers, but not in smokers. Smoking a single cigarette did not change preβ1-HDL concentrations or CHTpreβ1. Compared with nonsmokers, preβ1-HDL concentrations were relatively low in hyperlipidemic smokers but not in normolipidemic smokers, and CHTpreβ1was not a significant determinant of preβ1-HDL concentrations in smokers.
  
  
  
  Our findings suggest that smoking may be disadvantageous to individuals with hyperlipidemia because preβ1-HDL metabolism is altered.
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nagao
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirayama
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Kon
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Sasamoto
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Sugihara
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hirayama
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Isshiki
authors.affiliation: Niigata Prefectural Institute of Environmental Radiation Monitoring, Niigata Branch, Niigata, Niigata Japan.
authors.forename: Utako
authors.initials: U
authors.lastname: Seino
authors.affiliation: Tsukuba Research Institute, Sekisui Medical Co., Ltd, Ryugasaki, Ibaraki, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Miyazaki
authors.affiliation: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo, Japan. Electronic address: tmiida@juntendo.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
mesh_terms: D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000201:enzymology / Q000209:etiology; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism; D012306:Risk; D012907:Smoking / Q000009:adverse effects*
chemical_list: D008075:Lipoproteins, HDL; D007862:Phosphatidylcholine-Sterol O-Acyltransferase
keywords: ABCA1; Apolipoprotein AI; Cholesterol efflux; Dyslipidemia; HDL metabolism; LCAT; Prebeta-HDL