title: Involvement of miR-125a in resistance to daunorubicin by inhibiting apoptosis in leukemia cell lines.
abstract: In this study, we investigated whether miR-125a participated in the resistance of the leukemia cell lines to the chemotherapeutic agent daunorubicin. Higher expression of miR-125a is correlated with lower treatment response and shorter overall survival in acute leukemia patients. Overexpression of miR-125a induced drug resistance in HL-60, K562, and THP-1cell lines through reducing apoptosis. We also showed that miR-125a mediated daunorubicin resistance in leukemia cell lines through the decrease of GRK2 and Puma which were proved to be direct targets of miR-125a. This study may provide novel therapeutic targets for therapy and improve predictions of therapeutic responses in leukemia to daunorubicin.
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Bai
authors.affiliation: 2 Department of Hematology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Jieling
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Qin
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Chuxian
authors.initials: C
authors.lastname: Zhao
authors.affiliation: 1 Department of Hematology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000502:physiology; D045744:Cell Line, Tumor; D003630:Daunorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D005260:Female; D054769:G-Protein-Coupled Receptor Kinase 2 / Q000502:physiology; D006801:Humans; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000502:physiology
chemical_list: D051017:Apoptosis Regulatory Proteins; C438378:BBC3 protein, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; C496567:GRK2 protein, human; D054769:G-Protein-Coupled Receptor Kinase 2; D003630:Daunorubicin
keywords: MicroRNA-125a; apoptosis; daunorubicin; leukemia