title: Heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview.
abstract: Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom ofCrotalus durissus terrificussnakes and a fibrinogen-rich cryoprecipitate extracted from the blood ofBubalus bubalisbuffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its preclinical applications aiming at repairing nervous system traumas and regenerating bone marrow. Finally, we present an innovative safety trial I/II that found the product to be a safe and clinically promising candidate for treating chronic venous ulcers. A multicenter clinical trial, phase II/III, with a larger number of participants will be performed to prove the efficacy of an innovative biopharmaceutical product derived from animal venom.
authors.affiliation: Graduate Program in Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.|Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.|CEVAP/UNESP, Avenida José Barbosa de Barros, 1780, Botucatu, SP CEP 18610-307 Brazil.
authors.forename: Rui Seabra
authors.initials: RS
authors.lastname: Ferreira
authors.affiliation: Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Luciana Curtolo
authors.initials: LC
authors.lastname: de Barros
authors.affiliation: Department of Dermatology and Radiology, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Luciana Patrícia Fernandes
authors.initials: LPF
authors.lastname: Abbade
authors.affiliation: Department of Dermatology and Radiology, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Silvia Regina Catharino Sartori
authors.initials: SRCS
authors.lastname: Barraviera
authors.affiliation: Department of Dermatology and Radiology, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Maria Regina Cavariani
authors.initials: MRC
authors.lastname: Silvares
authors.affiliation: Graduate Program in Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.|Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Leticia Gomes
authors.initials: LG
authors.lastname: de Pontes
authors.affiliation: Graduate Program in Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.|Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Lucilene Delazari
authors.initials: LD
authors.lastname: Dos Santos
authors.affiliation: Graduate Program in Tropical Diseases, Botucatu Medical School, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.|Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors.forename: Benedito
authors.initials: B
authors.lastname: Barraviera
keywords: Buffaloes; Cryoprecipitate coagulum; Fibrin sealant; Snake venom; Thrombin-like enzyme