title: 3-Amino 1,8-naphthalimide, a structural analog of the anti-cholera drug virstatin inhibits chemically-biased swimming and swarming motility in vibrios.
abstract: A screen for inhibitors of Vibrio cholerae motility identified the compound 3-amino 1,8-naphthalimide (3-A18NI), a structural analog of the cholera drug virstatin. Similar to virstatin, 3-A18NI diminished cholera toxin production. In contrast, 3-A18NI impeded swimming and/or swarming motility of V. cholerae and V. parahemolyticus suggesting that it could target the chemotaxis pathway shared by the polar and lateral flagellar system of vibrios. 3-A18NI did not inhibit the expression of V. cholerae major flagellin FlaA or the assembly of its polar flagellum. Finally, 3-A18NI enhanced V. cholerae colonization mimicking the phenotype of chemotaxis mutants that exhibit counterclockwise-biased flagellum rotation.
authors.affiliation: Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, 720 Westview Dr., SW, Atlanta, GA 30310, USA.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, 720 Westview Dr., SW, Atlanta, GA 30310, USA.
authors.forename: Anisia J
authors.initials: AJ
authors.lastname: Silva
authors.affiliation: Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, 720 Westview Dr., SW, Atlanta, GA 30310, USA. Electronic address: jbenitez@msm.edu.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Benitez
mesh_terms: D015057:1-Naphthylamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002087:Butyrates / Q000494:pharmacology*; D002771:Cholera / Q000188:drug therapy*; D002772:Cholera Toxin / Q000096:biosynthesis; D005407:Flagella / Q000187:drug effects / Q000502:physiology; D005408:Flagellin / Q000378:metabolism; D051379:Mice; D053644:Naphthalimides / Q000494:pharmacology*; D015363:Quinolones / Q000494:pharmacology*; D014734:Vibrio cholerae / Q000187:drug effects* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D002087:Butyrates; D053644:Naphthalimides; D015363:Quinolones; C505381:virstatin; D005408:Flagellin; C086538:4-amino-1,8-naphthalimide; D002772:Cholera Toxin; D015057:1-Naphthylamine
keywords: Chemotaxis; Cholera toxin; Motility; Vibrio; Vibrio cholerae; Virstatin