title: The -2549 insertion/deletion polymorphism of VEGF gene associated with uterine leiomyoma susceptibility in women from Southeastern Iran.
abstract: Vascular endothelial growth factor (VEGF) is an important angiogenic factor that regulates angiogenesis and mediates sex steroid-induced cell growth. The present study investigated the association of VEGF gene-2578C/A (rs699947) and -2549 insertion/deletion polymorphisms in the promoter region of VEGF-A gene and uterine leiomyoma susceptibility in Southeast of Iran.
  
  
  
  One hundred and fifty five women with uterine leiomyoma and 157 age, BMI, and ethnicity matched healthy women were enrolled in this study. VEGF gene -2578C/A polymorphism genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the -2549 insertion/dele-tion polymorphism was analyzed by PCR method.
  
  
  
  The frequency of alleles and genotypes of VEGF-2578C/A polymorphism was not different between women with uterine leiomyoma and the controls; however, a significant association was revealed between II genotype of -2549 insertion/deletion (I/D) polymorphism of VEGF gene and uterine leiomyoma.
  
  
  
  The findings showed that VEGF gene -2549 insertion/deletion polymorphism was associated with uterine leiomyoma.
authors.forename: Farshid
authors.initials: F
authors.lastname: Keshavarzi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. sasalimi@yahoo.com.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.forename: Abbas
authors.initials: A
authors.lastname: Mohammadpour-Gharehbagh
authors.forename: Batool
authors.initials: B
authors.lastname: Teimoori
authors.forename: Atefeh
authors.initials: A
authors.lastname: Yazdi
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Farajian-Mashhadi
authors.forename: Minoo
authors.initials: M
authors.lastname: Yaghmaei
authors.forename: Mojgan
authors.initials: M
authors.lastname: Mokhtari
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran; D007889:Leiomyoma / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014594:Uterine Neoplasms / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: VEGF; polymorphism; uterine leiomyoma