title: New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS.
abstract: Hydrogen sulfide exists widely in mammalian tissues and plays a vital role in physiological and pathophysiological processes. However, striking differences with orders of magnitude were observed for the detected hydrogen sulfide concentrations in biological matrices among different measurements in literature, which lead to the uncertainty for examination the biological relevance of hydrogen sulfide. Here, we developed and validated a liquid chromatography- mass spectrometry (LC-MS/MS) method for the determination of hydrogen sulfide in various biological matrices by determination of a derivative of hydrogen sulfide and monobromobimane named sulfide dibimane (SDB).36S-labeled SDB was synthesized and validated for using as an internal standard. This method has been successfully used to measure hydrogen sulfide levels in a broad range of biological matrices, such as blood, plasma, tissues, cells, and enzymes, across different species. Moreover, a novel mode that hydrogen sulfide could loosely and non-covalently bind to human serum protein (HSA) and hemoglobin (HB) was revealed by using the developed method.
authors.affiliation: Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.|State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau.|Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.affiliation: Department of Physiology, Hebei Medical University, Hebei, China.|Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Sun
authors.affiliation: Institutes of Biological Sciences, Fudan University, Shanghai, China.|State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital of Fudan University, Shanghai, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Keke
authors.initials: K
authors.lastname: Huo
authors.affiliation: Analysis Center, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Zonglian
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Analysis Center, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Xin
authors.affiliation: Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Lilong
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Furong
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Instrumental Analysis Center, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Instrumental Analysis Center, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Fuyuan
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.|State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau.|Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Yi Zhun
authors.initials: YZ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D006862:Hydrogen Sulfide / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D032342:Muscle Cells / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D004718:Saccharomycetales / Q000201:enzymology; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D006862:Hydrogen Sulfide