title: "Cell Migration" Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach.
abstract: Maturation of cumulus-oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation and differentiation of cumulus cells. The migration of COCs to the oviduct after ovulation and the interaction between female gametes and/or embryos with maternal tissues are still poorly recognized on the molecular level. This study was aimed to first demonstrate the mRNA expression profile of cell migration markers during different stages of porcine oocytes maturation and developmental capability in vitro. The COCs were collected from a total of 45 pubertal crossbred Landrace gilts, brilliant cresyl blue (BCB) stained, and analyzed before (n = 150) or after (n = 150) in vitro maturation (IVM). Using the Affymetrix®Porcine Gene 1.1 ST Array, the expression profile of 12,258 porcine transcripts was examined. We found nine genes involved in cell migration mechanisms, that is, PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4. These genes were upregulated in porcine oocytes before IVM as compared with post-IVM expression analysis. Moreover, important mechanisms of biological interaction between VEGFA-KIT and VEGFA-INSR were also observed. The upregulation and/or downregulation of selected mRNAs expression after microarray assays was checked and approved by real-time quantitative polymerase chain reaction. We suggest that several genes, including LAMA2 or TPM1, encode proteins participating in the formation of the oocyte's protein architecture such as microtubules and kinetochore reorganization. As the expression of all "migration regulatory genes" investigated in this study was significantly upregulated in oocytes before IVM, we conclude that they may contribute to the maturational capability of porcine oocytes. However, migration potency of COCs is not accompanied by achievement of the MII stage by porcine oocytes in vitro. The investigated genes such as PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4 may be recognized as a new marker of porcine oocytes maturational competence during in vitro culture.
authors.affiliation: 1 Department of Anatomy, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Wiesława
authors.initials: W
authors.lastname: Kranc
authors.affiliation: 2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Joanna
authors.initials: J
authors.lastname: Budna
authors.affiliation: 2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Adrian
authors.initials: A
authors.lastname: Chachuła
authors.affiliation: 1 Department of Anatomy, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Sylwia
authors.initials: S
authors.lastname: Borys
authors.affiliation: 1 Department of Anatomy, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Artur
authors.initials: A
authors.lastname: Bryja
authors.affiliation: 3 Institute of Veterinary, Poznań University of Life Sciences , Poznań, Poland .
authors.forename: Marta
authors.initials: M
authors.lastname: Rybska
authors.affiliation: 2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Sylwia
authors.initials: S
authors.lastname: Ciesiółka
authors.affiliation: 2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Ewa
authors.initials: E
authors.lastname: Sumelka
authors.affiliation: 4 Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Brno, Czech Republic .
authors.forename: Michał
authors.initials: M
authors.lastname: Jeseta
authors.affiliation: 3 Institute of Veterinary, Poznań University of Life Sciences , Poznań, Poland .
authors.forename: Klaus P
authors.initials: KP
authors.lastname: Brüssow
authors.affiliation: 3 Institute of Veterinary, Poznań University of Life Sciences , Poznań, Poland .
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.affiliation: 3 Institute of Veterinary, Poznań University of Life Sciences , Poznań, Poland .
authors.forename: Paweł
authors.initials: P
authors.lastname: Antosik
authors.affiliation: 1 Department of Anatomy, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bruska
authors.affiliation: 2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: 2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.affiliation: 1 Department of Anatomy, Poznań University of Medical Science , Poznań, Poland .|2 Department of Histology and Embryology, Poznań University of Medical Science , Poznań, Poland .
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kempisty
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D059471:In Vitro Oocyte Maturation Techniques; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000235:genetics*; D013552:Swine; D059467:Transcriptome
keywords: cell migration; microarray; oocyte; pig