title: Improved Isolation, Proliferation, and Differentiation Capacity of Mouse Ovarian Putative Stem Cells.
abstract: The recent discovery of ovarian stem cells in postnatal mammalian ovaries, also referred to as putative stem cells (PSCs), and their roles in mammalian fertility has challenged the long-existing theory that women are endowed with a certain number of germ cells. The rare amount of PSCs is the major limitation for utilizing them through different applications. Therefore, this study was conducted in six phases to find a way to increase the number of Fragilis- and mouse vasa homolog (MVH)-positive sorted cells from 14-day-old NMRI strain mice. Results showed that there is a population of Fragilis- and MVH-positive cells with pluripotent stem cell characteristics, which can be isolated and expanded for months in vitro. PSCs increase their proliferation capacity under the influence of some mitogenic agents, and our results showed that different doses of stem cell factor (SCF) induce PSC proliferation with the maximum increase observed at 50 ng/mL. SCF was also able to increase the number of Fragilis- and MVH-positive cells after sorting by magnetic-activated cell sorting and enhance colony formation efficiency in sorted cells. Differentiation capacity assay indicated that there is a basic level of spontaneous differentiation toward oocyte-like cells during 3 days of culture. However, relative gene expression was significantly higher in the follicle-stimulating hormone-treated groups, especially in the Fragilis- sorted PSCs. We suggest that higher number of PSCs provides us either a greater source of energy that can be injected into energy-impaired oocytes in women with a history of repeat IVF failure or a good source for research.
authors.affiliation: 1 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Hossein
authors.initials: H
authors.lastname: Yazdekhasti
authors.affiliation: 2 Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Marzieh Agha
authors.initials: MA
authors.lastname: Hosseini
authors.affiliation: 1 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Zahra
authors.initials: Z
authors.lastname: Rajabi
authors.affiliation: 3 Department of Anatomy, School of Medicine, Alborz University of Medical Sciences , Karaj, Iran .
authors.forename: Soraya
authors.initials: S
authors.lastname: Parvari
authors.affiliation: 4 Department of Anatomy, School of Medical Sciences, Tarbiat Modarres University , Tehran, Iran .
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Salehnia
authors.affiliation: 5 Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences , Tehran, Iran .
authors.forename: Morteza
authors.initials: M
authors.lastname: Koruji
authors.affiliation: 6 PrimeGen Biotech , Irvine, California.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Izadyar
authors.affiliation: 1 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Fereshte
authors.initials: F
authors.lastname: Aliakbari
authors.affiliation: 1 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Mehdi
authors.initials: M
authors.lastname: Abbasi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D010053:Ovary / Q000166:cytology* / Q000187:drug effects; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D005640:Follicle Stimulating Hormone
keywords: FSH; infertility treatment; oocyte-like cells; ovarian stem cells; proliferation induction; stem cell factor