title: Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress.
abstract: Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency.
authors.affiliation: Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Sector-125 Campus, Noida, Uttar Pradesh, 201 313, India. rameshnp@gmail.com.|Department of Biological Sciences, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263 145, India. rameshnp@gmail.com.
authors.forename: Ramesh Namdeo
authors.identifier: http://orcid.org/0000-0003-0124-8537
authors.initials: RN
authors.lastname: Pudake
authors.affiliation: Department of Biological Sciences, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263 145, India.|School of Agriculture, Lovely Professional University, Jalandhar-Delhi G.T. Road (NH-1), Phagwara, Punjab, 144 402, India.
authors.forename: Chandra Mohan
authors.initials: CM
authors.lastname: Mehta
authors.affiliation: Department of Biological Sciences, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263 145, India.|School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Mohanta
authors.affiliation: Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Sector-125 Campus, Noida, Uttar Pradesh, 201 313, India.
authors.forename: Suvigya
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Sector-125 Campus, Noida, Uttar Pradesh, 201 313, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Varma
authors.affiliation: Department of Biological Sciences, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263 145, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Sharma
keywords: Arbuscular mycorrhiza; Gene expression; Phosphate transporter; Pi stress; Symbiosis