title: Recyclable magnetic carboxymethyl chitosan/calcium alginate - cellulase bioconjugates for corn stalk hydrolysis.
abstract: The use of cellulase hydrolysis of straw to produce fermentable sugars has many application prospects. However, cellulase is very expensive, which hampers its industrial applications. To improve cellulase's catalytic activity and reduce the enzyme cost, magnetite carboxymethyl chitosan/calcium alginate - cellulase bioconjugate (MCCCB) was synthesized via an improved hydrothermal method, molecular self-assembly technology, physical absorption, embedding and covalent bonding. Its loading capacity was 3.95mg/mL, and the catalytic activity increased to 267.18%. We decreased the release rate, improved the reusability, and enhanced the stability of MCCCB. Corn stalk hydrolysis also greatly improved, and the overall yield of fermentable sugars increased by 698.26%. All of these results indicate that MCCCB could significantly improve the efficiency of cellulase, greatly reduce the cost of enzyme, and effectively promote the production of fermentable sugars.
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China. Electronic address: jiangjianfang2014@163.com.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Jiang
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: He
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
authors.forename: Baodong
authors.initials: B
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: jianghao@126.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ao
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
authors.forename: Guangyan
authors.initials: G
authors.lastname: Xiang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002480:Cellulase; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006868:Hydrolysis; D003313:Zea mays
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; C514968:carboxymethyl-chitosan; D020723:Glucuronic Acid; D048271:Chitosan; D002480:Cellulase
keywords: Carboxymethyl chitosan; Cellulase; Corn stalk; Fermentable sugars; Sodium alginate