title: Crown discoloration promoted by materials used in regenerative endodontic procedures and effect of dental bleaching: spectrophotometric analysis.
abstract: OBJECTIVE
  To assess tooth crown's color after intracanal treatment with triple antibiotic paste (TAP) or calcium hydroxide (CH); cervical sealing with glass ionomer cement (GIC) or mineral trioxide aggregate (MTA); and bleaching with carbamide peroxide.
  
  
  METHODS
  After pulp removal and color spectrophotometer measurement, 50 bovine incisors were divided into 4 experimental groups and one control (untreated). Experiments were performed in phases (Ph). Ph1: TAP (ciprofloxacin, metronidazole, minocycline), TAPM (ciprofloxacin, metronidazole, amoxicillin), DAP (ciprofloxacin, metronidazole), or CH treatment groups. After 1 and 3 days (d); 1, 2, 3 weeks (w); and 1, 2, 3 and 4 months (m), color was measured and medications were removed. Ph2: GIC or MTA cervical sealing, each using half of the specimens from each group. Color was assessed after 1d, 3d; 1w, 2w, 3w; 1m and 2m. Ph3: Two bleaching sessions, each followed by color measurement. Data were analyzed with ANOVA and post-hoc Holm-Sidak method.
  
  
  RESULTS
  Ph1: Specimens of TAP group presented higher color alteration (ΔE) mean than those of TAPM group. No significant difference was found among TAP or TAPM and CH, DAP or Control groups. Ph2: cervical sealing materials showed no influence on color alteration. Ph3: Different ΔE means (from different groups), prior to bleaching, became equivalent after one bleaching session.
  
  
  CONCLUSIONS
  TAP induces higher color alteration than TAPM; color alteration increases over time; cervical sealing material has no influence on color alteration; and, dental bleaching was able to recover, at least partially, the tooth crown's color.
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Odontologia, Florianópolis, Santa Catarina, Brasil.
authors.forename: Luciane Geanini Pena Dos
authors.initials: LG
authors.lastname: Santos
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Odontologia, Florianópolis, Santa Catarina, Brasil.
authors.forename: Wilson Tadeu
authors.initials: WT
authors.lastname: Felippe
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Odontologia, Florianópolis, Santa Catarina, Brasil.
authors.forename: Beatriz Dulcineia Mendes de
authors.initials: BD
authors.lastname: Souza
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Informática e Estatística, Florianópolis, Santa Catarina, Brasil.
authors.forename: Andrea Cristina
authors.initials: AC
authors.lastname: Konrath
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Ciências Morfológicas, Florianópolis, Santa Catarina, Brasil.
authors.forename: Mabel Mariela Rodríguez
authors.initials: MM
authors.lastname: Cordeiro
authors.affiliation: Universidade Federal de Santa Catarina, Departamento de Odontologia, Florianópolis, Santa Catarina, Brasil.
authors.forename: Mara Cristina Santos
authors.initials: MC
authors.lastname: Felippe
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry; D002126:Calcium Hydroxide / Q000737:chemistry; D000077463:Carbamide Peroxide; D002417:Cattle; D004338:Drug Combinations; D005899:Glass Ionomer Cements / Q000737:chemistry; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D017797:Prosthesis Coloring; D012016:Reference Values; D015203:Reproducibility of Results; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012388:Root Canal Irrigants / Q000737:chemistry*; D012390:Root Canal Therapy / Q000379:methods*; D017640:Silicates / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors; D014073:Tooth Bleaching / Q000379:methods; D058205:Tooth Bleaching Agents / Q000737:chemistry*; D019228:Tooth Crown / Q000187:drug effects*; D014075:Tooth Discoloration / Q000139:chemically induced*; D014508:Urea / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D017607:Aluminum Compounds; D000900:Anti-Bacterial Agents; D017610:Calcium Compounds; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D010087:Oxides; D010545:Peroxides; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D017640:Silicates; D058205:Tooth Bleaching Agents; C086631:mineral trioxide aggregate; D000077463:Carbamide Peroxide; D014508:Urea; D002126:Calcium Hydroxide