title: Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells.
abstract: Pre-mRNA processing factor 19 (Prp19) is involved in many cellular events including pre-mRNA processing and DNA damage response. Recently, it has been identified as a candidate oncogene in hepatocellular carcinoma (HCC). However, the role of Prp19 in tumor biology is still elusive. Here, we reported that Prp19 arrested cell cycle in HCC cells via regulating G2/M transition. Mechanistic insights revealed that silencing Prp19 inhibited the expression of cell division cycle 5-like (Cdc5L) via repressing the translation of Cdc5L mRNA and facilitating lysosome-mediated degradation of Cdc5L in HCC cells. Furthermore, we found that silencing Prp19 induced cell cycle arrest could be partially resumed by overexpressing Cdc5L. This work implied that Prp19 participated in mitotic progression and thus could be a promising therapeutic target of HCC.
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China. 14111210006@fudan.edu.cn.
authors.forename: Renzheng
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China. xue.ruyi@zs-hospital.sh.cn.
authors.forename: Ruyi
authors.initials: R
authors.lastname: Xue
authors.affiliation: Key Laboratory of Medical Molecular Virology, Shanghai Medical College of Fudan University, Shanghai 200032, China. dqu@shmu.edu.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Qu
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China. yin.jie1@zs-hospital.sh.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China. shen.xizhong@zs-hospital.sh.cn.|Key Laboratory of Medical Molecular Virology, Shanghai Medical College of Fudan University, Shanghai 200032, China. shen.xizhong@zs-hospital.sh.cn.|Shanghai Institute of Liver Diseases, Zhongshan Hospital of Fudan University, Shanghai 200032, China. shen.xizhong@zs-hospital.sh.cn.
authors.forename: Xi-Zhong
authors.initials: XZ
authors.lastname: Shen
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D045643:DNA Repair Enzymes / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008247:Lysosomes / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000072260:RNA Splicing Factors / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C508357:CDC5L protein, human; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human
keywords: cell cycle; cell division cycle 5-like; lysosome-mediated degradation; pre-mRNA processing factor 19; repressing the translation of mRNA; translation