title: Vaccinium angustifoliumRoot Extract Suppresses FcɛRI Expression in Human Basophilic KU812F Cells.
abstract: Vaccinium angustifolium, commonly known as the lowbush blueberry, is a rich source of flavonoids, with which various human physiological activities have been associated. The present study focuses on the investigation of the effect of the methanolic extract of V. angustifolium root extract (VAE) on high affinity immunoglobulin E receptor (FcɛRI) α chain antibody (CRA-1)-induced allergic reaction in human basophilic KU812F cells. The total phenolic content of VAE was found to be 170±1.9 mg gallic acid equivalents/g. Flow cytometry analysis revealed that the cell surface expression of FcɛRI was suppressed in a concentration-dependent manner upon culture with VAE. Reverse-transcriptase polymerase chain reaction analysis showed that the mRNA level of the FcɛRI α chain was reduced in a concentration-dependent manner as a result of VAE treatment. Western blot analysis revealed that the protein expression of FcɛRI and the phosphorylation of extracellular signal-regulated kinases (ERK) 1/2 were concentration-dependently inhibited by VAE. We determined that VAE inhibited anti-CRA-1-induced histamine release, in addition to the elevation of intracellular calcium concentration ([Ca2+]i), in a concentration-dependent manner. These results indicate that VAE may exert an anti-allergic effect via the inhibition of calcium influx and histamine release, which occurs as a result of the down-regulation of FcɛRI expression through inhibition of ERK 1/2 activation.
authors.affiliation: College of Pharmacy, Sunchon National University, Jeonnam 57922, Korea.
authors.forename: Sun Yup
authors.initials: SY
authors.lastname: Shim
authors.affiliation: Department of Oriental Medicine Materials, Dongsin University, Jeonnam 58245, Korea.
authors.forename: Kyung Dong
authors.initials: KD
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Sunchon National University, Jeonnam 57922, Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Lee
keywords: ERK 1/2; FcɛRI; Vaccinium angustifolium; calcium influx; histamine