title: CT120: A New Potential Target for c-Myc in Head and Neck Cancers.
abstract: Background: CT120 is a universally expressed protein with seven transmembrane domains. It functions in cell proliferation, survival and apoptosis by activating Raf/MEK/ERK and PI3K/Akt signaling pathways. Evidence suggests that CT120 plays important roles in lung carcinogenesis and oncogenic pathway activation. c-Myc is an important transcription factor modulating cell progression, apoptosis and cellular transformation. Previous studies have shown thatMYCgene is amplified in many types of cancer including head and neck squamous cell carcinoma (HNSCC). Myc can regulate expression of many genes by binding to E-boxes. The aim of this study was to investigate the relationship between c-Myc protein andCT120gene.Methods:Tumor and normal tissue samples from 50 patients with HNSCC were investigated with chromatin immunoprecipitation assay (ChIP), Illumina MiSeq, bisulphite sequencing and qRT-PCR.Results:c-Myc binds to all E-boxes except E-box 5 onCT120promoter. The CpG dinucleotides were found to be partially methylated in all tumor and normal tissue samples. Bisulphite sequencing showed a 10% down-regulation in the methylation levels of the tumor tissues.CT120gene was hypomethylated and up-regulated in 56% of the tumor tissue samples. Expression of c-Myc was significantly higher in tumor tissues than in non-cancerous tissue samples.MYCwas overexpressed in 68% of the tumor tissue samples compared to normal tissues. The meanMYClevels were 2.42-fold higher in the tumor tissue samples. In 48% of the tumor tissues,MYCandCT120AmRNA were up- or down-regulated simultaneously (p<0.001).Conclusion:We show thatCT120gene is a target of c-Myc and it contributes to cancer progression in HNSCC.
authors.affiliation: Istanbul University Cerrahpasa Medical Faculty, Department of Medical Biology.
authors.forename: Elif
authors.initials: E
authors.lastname: Baltaci
authors.affiliation: Istanbul University Cerrahpasa Medical Faculty, Department of Medical Biology.
authors.forename: Betül
authors.initials: B
authors.lastname: Seyhan
authors.affiliation: Istanbul University Cerrahpasa Medical Faculty, Department of Medical Biology.
authors.forename: Onur
authors.initials: O
authors.lastname: Baykara
authors.affiliation: Istanbul University Cerrahpasa Medical Faculty, Department of Medical Biology.
authors.forename: Nur
authors.initials: N
authors.lastname: Buyru
keywords: CT120; FAM57A; HNSCC; bisulphite; c-Myc; methylation.; sequencing