title: Bletilla striataMicron Particles Function as a Hemostatic Agent by Promoting Rapid Blood Aggregation.
abstract: The human body cannot control blood loss without treatment. Available hemostatic agents are ineffective at treating cases of severe bleeding and are expensive or raise safety concerns.Bletilla striataserve as an inexpensive, natural, and promising alternative. However, no detailed studies on its hemostatic approach have been performed. The aim of this study was to examine the hemostatic effects ofB. striataMicron Particles (BSMPs) and their hemostatic mechanisms. We prepared and characterized BSMPs of different size ranges and investigated their use as hemostatic agent. BSMPs of different size ranges were characterized by scanning electron microscope. In vitro coagulation studies revealed BSMP-blood aggregate formation via stereoscope and texture analyzers. In vivo studies based on rat injury model illustrated the BSMP capabilities under conditions of hemostasis. Compared to other BSMPs of different size ranges, BSMPs of 350-250μm are most efficient in hemostasis. As powder sizes decrease, the degree of aggregation between particles and hemostatic BSMP effects declines. The BSMP in contact with a bleeding surface locally forms a visible particle/blood aggregate as a physical barrier that facilitates hemostasis. Considering the facile preparation, low cost, and long shelf life ofB. striata, BSMPs offer great potential as mechanisms of trauma treatment.
authors.affiliation: The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base Co-Founded by Sichuan Province and MOST, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zeng
authors.affiliation: The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base Co-Founded by Sichuan Province and MOST, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Zhencheng
authors.initials: Z
authors.lastname: Liao
authors.affiliation: The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base Co-Founded by Sichuan Province and MOST, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Chaomei
authors.initials: C
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Hanshuo
authors.identifier: 0000-0003-3173-2338
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base Co-Founded by Sichuan Province and MOST, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Yan
authors.identifier: 0000-0001-5209-0261
authors.initials: Y
authors.lastname: Qu