title: Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy.
abstract: The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku, 20520, Finland. tibor.veres@utu.fi.
authors.forename: Tibor Z
authors.identifier: http://orcid.org/0000-0002-6980-8900
authors.initials: TZ
authors.lastname: Veres
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku, 20520, Finland.
authors.forename: Tamás
authors.initials: T
authors.lastname: Kopcsányi
authors.affiliation: Institute of Biomedicine, Turku Center for Disease Modeling, and Turku PET Centre, University of Turku, Turku, 20520, Finland.
authors.forename: Marko
authors.initials: M
authors.lastname: Tirri
authors.affiliation: Department of Preclinical Pharmacology and In Vitro Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, and Institute of Immunology, MHH; Hannover, 30625 Germany; Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Centre for Lung Research (DZL), Hannover, Germany.
authors.forename: Armin
authors.initials: A
authors.lastname: Braun
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku, 20520, Finland.|WPI Immunology Frontier Research Center, Osaka University, Osaka, 565-0871, Japan.
authors.forename: Masayuki
authors.identifier: http://orcid.org/0000-0001-6122-5201
authors.initials: M
authors.lastname: Miyasaka
authors.affiliation: Lymphocyte Biology Section, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Germain
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku, 20520, Finland.
authors.forename: Sirpa
authors.initials: S
authors.lastname: Jalkanen
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku, 20520, Finland.|Department of Medical Microbiology and Immunology, University of Turku, Turku, 20520, Finland.
authors.forename: Marko
authors.initials: M
authors.lastname: Salmi
mesh_terms: D000818:Animals; D002923:Cilia; D006801:Humans; D007091:Image Processing, Computer-Assisted; D000069416:Intravital Microscopy / Q000295:instrumentation / Q000379:methods; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods; D009079:Mucociliary Clearance; D020545:Respiratory Mucosa / Q000166:cytology* / Q000000981:diagnostic imaging*; D014132:Trachea / Q000166:cytology* / Q000000981:diagnostic imaging*; D055114:X-Ray Microtomography