title: Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing.
abstract: The Cannonball jellyfish (Stomolophus sp.) is a species of jellyfish with high relevance in artisanal fishing. Studies of their populations do not extend beyond the morphological descriptions knowing that presents a great morphological variability. However, there are no genetic studies to determine the number of independent populations, so microsatellite markers become a suitable option. Since there are no species-specific microsatellite loci, in this paper, 14 new microsatellite loci are characterized. Microsatellite loci were isolated de novo through next generation sequencing, by two runs on Illumina MiSeq. A total of 506,771,269 base pair were obtained, from which 142,616 were microsatellite loci, and 1546 of them could design primers. We tested 14 primer pairs on 32 individuals from Bahía de La Paz, Gulf of California. We observed low genetic variation among loci (mean number of alleles per locus = 4.33, mean observed heterozygosity 0.381, mean expected heterozygosity 0.501). These loci are the first ones described for the species and will be helpful to carry out genetic diversity and population genetics studies.
authors.affiliation: Laboratorio de Genética para la Conservación, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., México.|Centro para el Estudio de Sistemas Marinos, CESIMAR (CCT CENPAT-CONICET), Blvd. Brown 2915, U9120ACD, Puerto Madryn, Chubut, Argentina.
authors.forename: Leandro Nicolás
authors.initials: LN
authors.lastname: Getino-Mamet
authors.affiliation: Laboratorio de Genética para la Conservación, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., México.
authors.forename: Tania
authors.initials: T
authors.lastname: Valdivia-Carrillo
authors.affiliation: School of Natural Sciences, University of California, Merced, 5200 North Lake Road, Merced, 95343, USA.
authors.forename: Liza
authors.initials: L
authors.lastname: Gómez Daglio
authors.affiliation: Laboratorio de Genética para la Conservación, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., México. fgarciadl@cibnor.mx.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: García-De León
mesh_terms: D000483:Alleles; D000818:Animals; D017931:DNA Primers; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005828:Genetics, Population / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D018895:Microsatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D037401:Scyphozoa / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers
keywords: Cannonball jellyfish; Gulf of California; Microsatellite primers; Next generation sequencing; Stomolophidae; Stomolophus sp.