title: Effect of sulfamethoxazole on aerobic denitrification by strain Pseudomonas stutzeri PCN-1.
abstract: Sulfamethoxazole (SMX), as a common sulfonamide antibiotic, was reported to affect conventional anaerobic denitrification. This study presented effects of SMX on aerobic denitrification by an aerobic denitrifier strain Pseudomonas stutzeri PCN-1. Results demonstrated serious inhibition of N2O reduction as SMX reached 4μg/L, leading to higher N2O emission ratio (251-fold). Increase of SMX (∼8μg/L) would induce highest nitrite accumulation (95.3mg/L) without reduction, and severe inhibition of nitrate reduction resulted in lower nitrate removal rate (0.15mg/L/h) as SMX reached 20μg/L. Furthermore, corresponding inhibition of SMX on denitrifying genes expression (nosZ>nirS>cnorB>napA) was found with a time-lapse expression between nosZ and cnorB. Meanwhile, the decline in electron transport activity and active microbial biomass of strain PCN-1 was revealed. The insight into mechanism of SMX influence on aerobic denitrifier is of particular significance to upgrade nitrogen removal process in antibiotics-containing wastewater treatment plant.
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Gui
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China. Electronic address: nijinren@iee.pku.edu.cn.
authors.forename: Jinren
authors.initials: J
authors.lastname: Ni
mesh_terms: D000332:Aerobiosis; D058440:Denitrification / Q000187:drug effects*; D011285:Pregnenolone Carbonitrile; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D013420:Sulfamethoxazole / Q000494:pharmacology
chemical_list: D011285:Pregnenolone Carbonitrile; D013420:Sulfamethoxazole
keywords: Aerobic denitrification; ETSA; Gene expression; Pseudomonas stutzeri; SMX; Sensitivity