title: Bacteria can proliferate in thawed cryoprecipitate stored at room temperature for longer than 4 h.
abstract: Although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. Here, we separately spiked thawed cryoprecipitate units with four bacterial strains: Staphylococcus epidermidis, Serratia liquefaciens, Pseudomonas putida and Pseudomonas aeruginosa. No strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. Pathogen inactivation technologies could be explored to mitigate the safety risk posed by extending storage of thawed cryoprecipitate at room temperature.
authors.affiliation: Centre for Innovation, Canadian Blood Services, Ottawa, ON, Canada.
authors.forename: S
authors.identifier: http://orcid.org/0000-0003-0705-4987
authors.initials: S
authors.lastname: Ramirez-Arcos
authors.affiliation: Centre for Innovation, Canadian Blood Services, Ottawa, ON, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Jenkins
authors.affiliation: Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada.|Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: W P
authors.initials: WP
authors.lastname: Sheffield
mesh_terms: D016470:Bacteremia / Q000517:prevention & control; D058731:Blood Safety; D015925:Cryopreservation; D005615:Freezing; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D016958:Pseudomonas putida / Q000254:growth & development; D044090:Serratia liquefaciens / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000254:growth & development
keywords: bacteria; cryoprecipitate; safety