title: Rods-on-sphere silica particles for high performance liquid chromatography.
abstract: Silica spheres covered with rods perpendicular to the particle surface were prepared by a simple one-pot sol-gel process. Thus prepared rods-on-sphere silica particles possessed core-shell structure. Compared to other core-shell silica particles in which the shell was synthesized by the time-consuming multiple-step layer-by-layer coating technique, the shell of our rods-on-sphere particles was formed by directly grown rods from the silica spheres. The coverage of the rods on the particle surface could be tuned by changing the amount of water in the reaction. The rods on the particle surface increased the surface roughness which may help decreasing the A-term. Therefore, the calcined and modified rods-on-sphere silica particles were packed into stainless steel columns and then assessed for the separation of various samples including small molecules and proteins. In comparison with a commercially available Kromasil column, the pressure of the rods-on-sphere column is much lower under the same separation conditions, while the column efficiency was comparable. The separation results demonstrate that rods-on-sphere silica particles are a type of new and highly promising packing stationary phase for high performance liquid chromatography.
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China. Electronic address: quqishu@qq.com.
authors.forename: Qishu
authors.initials: Q
authors.lastname: Qu
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Han
authors.initials: H
authors.lastname: Xuan
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Kehua
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Siying
authors.initials: S
authors.lastname: Sun
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Shaojie
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000814:Aniline Compounds / Q000032:analysis / Q000302:isolation & purification; D002722:Chlorobenzenes / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009281:Naphthalenes / Q000032:analysis / Q000302:isolation & purification; D010316:Particle Size; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013806:Theophylline / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000814:Aniline Compounds; D002722:Chlorobenzenes; D009281:Naphthalenes; D011506:Proteins; C031721:naphthalene; C008625:nitroaniline; C004726:2-dichlorobenzene; D012822:Silicon Dioxide; D013806:Theophylline
keywords: HPLC; Particle; Rods-on-sphere; Silica