title: Monoterpenoid indole alkaloids from Alstonia mairei and their cytotoxicity.
abstract: Phytochemical investigation on the 70% ethanol extract of the leaves of Alstonia mairei resulted in the isolation of three new monoterpenoid indole alkaloids, alstomairines A-C (1-3), along with one known compound, alpneumine A (4). Structural elucidation of all the compounds was accomplished by spectral methods such as 1D and 2D NMR, IR, UV, and HRESIMS. The isolated compounds were tested in vitro for cytotoxic activities against four osteosarcoma cell lines. Consequently, alkaloids 2 and 3 exhibited cytotoxic activities for all tested tumor cell lines with IC50values from 9.2 to 13.0 μM.
authors.affiliation: a Department of Basic Medicine , Qujing Medical College , Qujing 655000 , China.
authors.forename: Ting-Liang
authors.initials: TL
authors.lastname: Yan
authors.affiliation: b Department of Spine Surgery , 107th Hospital of People's Liberation Army , Yantai , China.
authors.forename: Dun-Xin
authors.initials: DX
authors.lastname: Han
authors.affiliation: c College of Biological Resources and Environment Science , Qujing Normal University , Qujing 655011 , China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: a Department of Basic Medicine , Qujing Medical College , Qujing 655000 , China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Huang
authors.affiliation: d Department of Plastic and Burns Surgery , Fujian Dongfang Hospital, Xiamen University , Fuzhou 350025 , China.
authors.forename: Hong-Kun
authors.initials: HK
authors.lastname: Wang
mesh_terms: D031051:Alstonia / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D046948:Secologanin Tryptamine Alkaloids; C000621771:alstomairine B; C000621772:alstomairine C
keywords: Alstonia mairei; Apocynaceae; cytotoxicity; monoterpenoid indole alkaloids