title: Serological Evidence for the Circulation of Rift Valley Fever Virus in Domestic Small Ruminants in Southern Gabon.
abstract: Rift Valley fever (RVF) is a zoonotic disease, which caused several epidemics in humans in many countries of Africa. Using an inhibition enzyme-linked immunosorbent assay (ELISA), real-time reverse transcription PCR, and nested one-step reverse transcription PCR, we conducted a cross-sectional study in populations of sheep and goats from the Mongo County in 2014 to determine the circulation of the Rift Valley fever virus (RVFV) in small ruminants from this area. From a total of 201 small ruminants (95 sheep and 106 goats), the overall IgG seroprevalence against the RVFV was 6.47% (13/201). No RVFV RNA was detected in the animal plasmas. Logistic regression analysis showed that age, species, sex, and locality were not the significant risk factors. The findings of this study highlight the risk of RVF for domestic ruminants bred in this region and for the human rural population living in contact with these animals and they emphasize the need to develop adequate control measures to limit this threat.
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .|2 Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB) , Franceville, Gabon .
authors.forename: Gael Darren
authors.initials: GD
authors.lastname: Maganga
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .
authors.forename: Andre Lea
authors.initials: AL
authors.lastname: Abessolo Ndong
authors.affiliation: 2 Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB) , Franceville, Gabon .
authors.forename: Clency Sylde
authors.initials: CS
authors.lastname: Mikala Okouyi
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .
authors.forename: Sheila
authors.initials: S
authors.lastname: Makiala Mandanda
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .
authors.forename: Nadine
authors.initials: N
authors.lastname: N'Dilimabaka
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .
authors.forename: Anais
authors.initials: A
authors.lastname: Pinto
authors.affiliation: 3 SIAT-Gabon Group , Libreville, Gabon .
authors.forename: Ernest
authors.initials: E
authors.lastname: Agossou
authors.affiliation: 3 SIAT-Gabon Group , Libreville, Gabon .
authors.forename: Brieuc
authors.initials: B
authors.lastname: Cossic
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Akue
authors.affiliation: 1 Centre International de Recherches Médicales de Franceville (CIRMF) , Franceville, Gabon .|4 UMR 224 (MIVEGEC: Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle), Institut de Recherche pour le Développement (IRD) , IRD/CNRS/UM1/UM2, Montpellier, France .
authors.forename: Eric Maurice
authors.initials: EM
authors.lastname: Leroy
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005260:Female; D005681:Gabon / Q000453:epidemiology; D015511:Goat Diseases / Q000097:blood* / Q000453:epidemiology; D006041:Goats; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D012367:RNA, Viral / Q000097:blood; D012295:Rift Valley Fever / Q000097:blood* / Q000453:epidemiology; D012296:Rift Valley fever virus / Q000302:isolation & purification*; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood* / Q000453:epidemiology; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral
keywords: Gabon; IgG antibodies; RVFV; goats; sheep