title: Metallothionein 1M suppresses tumorigenesis in hepatocellular carcinoma.
abstract: Members of the metallothionein (MT) family are involved in metal detoxifcation and in the protection of cells against certain electrophilic carcinogens. In present study, it was found that MT1M was downregulated in more than 77.1% (91/118) of hepatocellular carcinoma (HCC) tissues compared with adjacent non-tumor tissues. Furthermore, overexpression of MT1M inhibited cell viability, colony formation, cell migration and invasion in HCC cell lines and tumor cell growth in xenograft nude mice, and activated cell apoptosis in HCC cell lines. In addition, immunohistochemistry analysis showed MT1M was negative or weak staining in tumor tissues but moderate or strong staining in adjacent non-tumor tissues. The sensitivity and specificity of MT1M for HCC diagnosis were 76.27% and 89.83%, respectively. In conclusion, MT1M was identified as a potential tumor marker for HCC and may serve as a useful therapeutic agent for HCC gene therapy.
authors.affiliation: Department of Pathology, The First Hospital of Taizhou, Wenzhou Medical University, Taizhou 318020, China.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Fu
authors.affiliation: Department of Pathology, The First Hospital of Taizhou, Wenzhou Medical University, Taizhou 318020, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Pan
authors.affiliation: Department of Pathology, The First Hospital of Taizhou, Wenzhou Medical University, Taizhou 318020, China.
authors.forename: Ju-Hua
authors.initials: JH
authors.lastname: Pan
authors.affiliation: Department of Pathology, Taizhou Hospital, Wenzhou Medical University, Taizhou Hospital of Zhejiang Province, Taizhou Enze Medical Center (Group), Linhai 317000, China.
authors.forename: Mei-Fu
authors.initials: MF
authors.lastname: Gan
mesh_terms: D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation
chemical_list: D014408:Biomarkers, Tumor; C000595829:MT1M protein, human; D008668:Metallothionein
keywords: MT1M; hepatocellular carcinoma; metallothionein 1M; tumor marker