title: Increased electrocatalyzed performance through high content potassium doped graphene matrix and aptamer tri infinite amplification labels strategy: Highly sensitive for matrix metalloproteinases-2 detection.
abstract: Herein, a super-labeled immunoassay was fabricated for matrix metalloproteinases-2 detection. A self-corrosion ITO micro circuit board was designed in this sensing platform to reduce the random error in the same testing condition, and the self-constructed sensing platform is portable with a cheap price. The K-modified graphene (K-GS) was utilized as the matrix material, which was synthesized well by phenylate and phenanthrene through the polar bond of nonpolar molecule phenylate and the π-π interaction for the first time. An aptamer-based labels based on Au nanoparticles (AuNPs), thionine (Th) and horseradish peroxidase (HRP) were applied as the signal source for tri infinite amplification. This fabricated super-labeled immunoassay exhibit excellent performance for MMPs-2 detection. It displayed a broad linear range of 10-4-10ng/mL with a low detection limit of 35 fg/mL, which may have a potential application in the clinical diagnose.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Wanruo
authors.initials: W
authors.lastname: Lou
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011188:Potassium / Q000737:chemistry
chemical_list: D052158:Aptamers, Peptide; D006046:Gold; D006108:Graphite; D006735:Horseradish Peroxidase; D020778:Matrix Metalloproteinase 2; D011188:Potassium
keywords: Aptamer; Immunoassay; Matrix metalloproteinases-2; Potassium doped graphene; Tri infinite amplification