title: Multi-organ toxicity induced by fine particulate matter PM2.5in zebrafish (Danio rerio) model.
abstract: The fine particulate matter (PM2.5) in air pollution is a major public health concern and now known to contribute to severe diseases, therefore, a comprehensive understanding of PM2.5-induced adverse effects in living organisms is needed urgently. This study was aimed to evaluate the toxicity of PM2.5on multi-organ systems in a zebrafish (Danio rerio) model. The embryonic toxicity induced by PM2.5was demonstrated by an increase in mortality and inhibition of hatching rate, in a dose- and time-dependent manner. PM2.5caused the pericardial edema, as well as reducing heart rate and cardiac output. The area of sub-intestinal vessels (SIVs) was significant reduced in Tg(fli-1:EGFP) transgenic zebrafish lines. Morphological defects and yolk sac retention were associated with hepatocyte injury. In addition, PM2.5disrupted the axonal integrity, altering of axon length and pattern in Tg(NBT:EGFP) transgenic lines. Genes involved in cardiac function (spaw, supt6h, cmlc1), angiogenesis (vegfr2a, vegfr2b), and neural function (gabrd, chrna3, npy8br) were markedly down-regulated; while genes linked to hepatic metabolism (cyp1a, cyp1b1, cyp1c1) were significantly up-regulated by PM2.5. In summary, our data showed that PM2.5induced the cardiovascular toxicity, hepatotoxicity and neurotoxicity in zebrafish, suggested that PM2.5could cause multi-organ toxicity in aquatic organism.
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Junchao
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Hejing
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Shi
authors.affiliation: University/BHF Centre for Cardiovascular Science, Queens Medical Research Institute, The University of Edinburgh, Edinburgh, UK. Electronic address: mark.miller@ed.ac.uk.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Miller
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, PR China. Electronic address: zwsun@ccmu.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000397:Air Pollution / Q000009:adverse effects; D000818:Animals; D002319:Cardiovascular System; D065633:Cytochrome P-450 CYP1B1; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian; D052638:Particulate Matter / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1
keywords: Air pollution; Cardiovascular toxicity; Hepatotoxicity; Neurotoxicity; PM(2.5); Zebrafish