title: Potential biomarkers as an indicator of vertical transmission of Johne's disease in a Korean native cattle farm.
abstract: Paratuberculosis (PTB) is caused byMycobacterium aviumsubsp.paratuberculosis(MAP) and is one of the most widespread and economically important diseases in cattle. After birth, calves are raised with natural breast feeding without separation from their mothers in most Korean native cattle (Hanwoo breed) farms. Vertical transmission of PTB has been reported, but the exact PTB infection route has not been revealed in Hanwoo farms. Calves of MAP seropositive dams were tested for MAP presence and MAP antibodies in feces and tissues. MAP was detected in calf tissues by using polymerase chain reaction. Expressions of genes reported to be prognostic biomarkers of MAP infection changed in both calves and cows (p＜ 0.05). Expression of two genes (HGFandSERPINE1) were significantly decreased in MAP-infected cattle and their offspring (p＜ 0.01). The results suggest that biomarker gene expression profiles can be useful in detecting early stage MAP infection. Based on the results, complete eradication of MAP may be possible if accurate diagnostic methods to detect infected calves are added to the current PTB eradication strategy, which, because infected individuals are likely to develop into fecal MAP shedders at any time, includes isolation of new born calves and feeding sterilized colostrum.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Hong-Tae
authors.initials: HT
authors.lastname: Park
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Hyun-Eui
authors.initials: HE
authors.lastname: Park
authors.affiliation: Department of Animal Science & Technology, Suncheon National University, Suncheon 57922, Korea.
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Cho
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Pyeongchang 25340, Korea.
authors.forename: Eui-Hyung
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Myunghwan
authors.initials: M
authors.lastname: Jung
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Su-Hyung
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000635:transmission*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics*; D010283:Paratuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000635:transmission*; D011379:Prognosis; D056910:Republic of Korea / Q000453:epidemiology; D059467:Transcriptome
chemical_list: D005819:Genetic Markers
keywords: Korean native cattle; Mycobacterium avium subsp. paratuberculosis; biomarker; subclinical infection; vertical transmission