title: Protective efficacy of Zika vaccine in AG129 mouse model.
abstract: Zika virus (ZIKV) is a mosquito-borne flavivirus that causes asymptomatic infection or presents only mild symptoms in majority of those infected. However, vaccination for ZIKV is a public health priority due to serious congenital and neuropathological abnormalities observed as a sequelae of the virus infection in the recent epidemics. We have developed an inactivated virus vaccine with the African MR 766 strain. Here we show that two doses of the vaccine provided 100% efficacy against mortality and disease following challenge with homotypic MR 766 and the heterotypic FSS 13025 ZIKV strains in the Type I and Type II interferon deficient AG129 mice. Two doses of the vaccine elicited high titer of neutralizing antibodies in Balb/c mice, and the vaccine antisera conferred protection against virus challenge in passively immunized mice. The studies were useful to rationalize vaccine doses for protective efficacy. Furthermore, the vaccine antisera neutralized the homotypic and heterotypic ZIKV strains in vitro with equivalent efficiency. Our study suggests a single ZIKV serotype, and that the development of an effective vaccine may not be limited by the choice of virus strain.
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: K
authors.initials: K
authors.lastname: Sumathy
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Bharathi
authors.initials: B
authors.lastname: Kulkarni
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Ravi Kumar
authors.initials: RK
authors.lastname: Gondu
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Sampath Kumar
authors.initials: SK
authors.lastname: Ponnuru
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Nagaraju
authors.initials: N
authors.lastname: Bonguram
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Eligeti
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Sindhuja
authors.initials: S
authors.lastname: Gadiyaram
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Usha
authors.initials: U
authors.lastname: Praturi
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Bhushan
authors.initials: B
authors.lastname: Chougule
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Latha
authors.initials: L
authors.lastname: Karunakaran
authors.affiliation: R&D Department Bharat Biotech International Ltd. Genome Valley, Shamirpet Hyderabad - 500 078, India.
authors.forename: Krishna M
authors.initials: KM
authors.lastname: Ella
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D004195:Disease Models, Animal; D051379:Mice; D016896:Treatment Outcome; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000627:therapeutic use*; D000071244:Zika Virus / Q000276:immunology*; D000071243:Zika Virus Infection / Q000517:prevention & control*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines