title: The value of applying a melatonin antagonist (Luzindole) in improving the success rate of the bipedal rat scoliosis model.
abstract: An ideal animal model has always been the key to research the pathogenesis and treatment of adolescent idiopathic scoliosis (AIS), while available methods have obvious disadvantages. The deficiency of melatonin has been proved relating to AIS. In this research, we intended to apply Luzindole, the melatonin antagonist, in bipedal rat model, for the block of combination of melatonin and its receptor, to inhibit the melatonin effect, and then to understand whether this method can effectively improve the scoliosis rate of bipedal rat model, and investigate the role of melatonin in scoliosis. To investigate the feasibility of improving the success rate of bipedal rat scoliosis model via intraperitoneal injection of melatonin antagonist (Luzindole).
  
  
  
  A total of 60 3-weeks-old Sprague-Dawley rats were included in this study, and were divided into 3 groups (A, B and C). Each group included 20 rats. Osteotomy of the bilateral proximal humerus and proximal tailbone was performed in group A and group B; intraperitoneal injection of Luzindole (0.2 mg/kg) was performed in group A and group C. X-rays were taken before the surgery, 1 month after the surgery, 3 months after the surgery, and 6 months after the surgery, to calculate the Cobb's angle of the spine (>10° was considered scoliosis). The weight of every rat was also measured at the same time. Rats were euthanized 6 months after surgery to determine the calmodulin level in thrombocytes.
  
  
  
  The rate of scoliosis in group A (14/20) was significantly higher than those in group B (6/20) and group C (0/20) (P < 0.05). The differences in the weights of the 3 groups were non-significant; as were differences in the calmodulin level in thrombocytes.
  
  
  
  The application of the melatonin antagonist of Luzindole can improve the success rate of the bipedal rat scoliosis model. Meanwhile, this study indicates that a decreased melatonin level is not the primary cause of scoliosis, but that it may increase the likelihood and severity of scoliosis.
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.|Department of Orthopedics, The Fifth People's Hospital, Fudan University, No.128, Ruili Road, Shanghai, 200240, China.
authors.forename: Feizhou
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Orthopedics, Huashan Hospital, Fudan University, No.12, Middle Wulumuqi Road, Shanghai, 200040, China. mxs893@sina.com.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008550:Melatonin / Q000037:antagonists & inhibitors*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012600:Scoliosis / Q000209:etiology*; D014363:Tryptamines
chemical_list: D014363:Tryptamines; C057154:luzindole; D008550:Melatonin
keywords: Bipedal rat model; Melatonin antagonist; Scoliosis