title: A Comparative Study of Oral Microbiota in Infants with Complete Cleft Lip and Palate or Cleft Soft Palate.
abstract: Few reports have been published on the early microbiota in infants with various types of cleft palate. We assessed the formation of the oral microbiota in infants with complete cleft lip and palate (CLPn= 30) or cleft soft palate (CSPn= 25) in the neonatal period (T1 time) and again in the gum pad stage (T2 time). Culture swabs from the tongue, palate, and/or cleft margin at T1 and T2 were taken. We analysed the prevalence of the given bacterial species (the percentage) and the proportions in which the palate and tongue were colonised by each microorganism. At T1,Streptococcus mitis (S. mitis)were the most frequently detected in subjects with CLP or CSP (63% and 60%, resp.). A significantly higher frequency of methicillin-sensitiveStaphylococcus aureus(S. aureusMSSA) was observed in CLP compared to the CSP group. At T2, significantly higher percentages ofS. mitis,S. aureusMSSA,Staphylococcus epidermidis, and members of the Enterobacteriaceae family were noted in CLP infants compared to the CSP.S. mitisandStreptococcus sanguinisappeared with the greatest frequency on the tongue, whereasStreptococcus salivariuswas predominant on the palate. The development of the microbiota in CLP subjects was characterised by a significant increase in the prevalence of pathogenic bacteria.
authors.affiliation: Department of Orthodontics, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Traugutta Square 2, 41-800 Zabrze, Poland.
authors.forename: Agnieszka
authors.identifier: 0000-0003-3894-1298
authors.initials: A
authors.lastname: Machorowska-Pieniążek
authors.affiliation: Department of Microbiology and Immunology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Anna
authors.identifier: 0000-0003-0796-8818
authors.initials: A
authors.lastname: Mertas
authors.affiliation: Department of Conservative Dentistry with Endodontics, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Akademicki Square 17, 41-902 Bytom, Poland.
authors.forename: Małgorzata
authors.identifier: 0000-0003-0584-968X
authors.initials: M
authors.lastname: Skucha-Nowak
authors.affiliation: Department of Conservative Dentistry with Endodontics, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Akademicki Square 17, 41-902 Bytom, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Tanasiewicz
authors.affiliation: Department of Dental Surgery, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Akademicki Square 17, 41-902 Bytom, Poland.
authors.forename: Tadeusz
authors.identifier: 0000-0002-2283-5784
authors.initials: T
authors.lastname: Morawiec
mesh_terms: D002971:Cleft Lip / Q000382:microbiology* / Q000473:pathology; D002972:Cleft Palate / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification / Q000472:pathogenicity; D064307:Microbiota; D010160:Palate, Soft / Q000002:abnormalities* / Q000382:microbiology / Q000473:pathology; D014059:Tongue / Q000166:cytology / Q000382:microbiology