title: Inhibiting Cytochrome C Oxidase Leads to Alleviated Ischemia Reperfusion Injury.
abstract: OBJECTIVE
  The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways.
  
  
  METHODS
  We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial H2O2and mitochondrial complex activity.
  
  
  RESULTS
  KCN pre-treatment led to decreased infarction area after IR injury and improved cardiac function. KCN pre-treated group challenged with IR injury was associated with reduced ROS production through inhibition of activity and not downregulation of CcO expression. In addition, KCN pre-treatment was associated with enhanced expression and activity of mitochondrial antioxidase, suggesting the role of CcO in regulating IR injury through oxidative stress.
  
  
  CONCLUSIONS
  KCN pre-treatment reduced the severity of IR injury. The potential mechanism could be increased endogenous anti-oxidase activity and consequently, the enhanced clearance of ROS.
authors.affiliation: Department of Anesthesia, Xiang-Ya Second Hospital, Central South University, Changsha, China.
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, the Second Affiliated Hospital, University of South China, Hengyang, China.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Duan
authors.affiliation: Department of Anesthesiology, Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Anesthesia, Xiang-Ya Second Hospital, Central South University, Changsha, China.
authors.forename: Junmei
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Anesthesia, Xiang-Ya Second Hospital, Central South University, Changsha, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
keywords: Cytochrome C oxidase; Ischemia-reperfusion injury; Mitochondria; Myocardial infarction