title: Gallic Acid and Dodecyl Gallate Prevents Carbon Tetrachloride-Induced Acute and Chronic Hepatotoxicity by Enhancing Hepatic Antioxidant Status and Increasing p53 Expression.
abstract: Gallic acid (3,4,5-trihydroxybenzoic acid, GA), a natural phenolic acid has been reported as a strong antioxidant. Therefore the present study was designed to evaluate the effects of GA and dodecyl gallate (DGA) against acute and chronic carbon tetrachloride (CCl4)-induced hepatotoxicity. For acute model, rats were orally treated with GA and DGA for 7 d prior to CCl4by intraperitoneally (i.p.) injection. For the chronic model, rats were orally treated with GA or DGA and CCl4i.p. twice a week for four weeks. In both acute and chronic models, the CCl4-treated groups showed significantly increase in serum hepatic enzyme activities and histopathologic alterations, as well as a disruption in antioxidative status. In contrast, the treatment with GA and DGA restored serum hepatic enzymes activities, improved histopathologic alterations, increased glutathione (GSH) and decreased lipid peroxidation levels. The activities of liver antioxidant enzymes were increased by GA and DGA only in acute model. The expression of p53 gene increased about 3.5 times after GA and DGA treatments, which could result in cell death of damaged hepatocytes preventing of a lifelong liver failure. Thus, these results suggest that GA and DGA has the potential to prevent liver damages as the case of fibrosis condition.
authors.affiliation: Núcleo de Biotecnologia, Universidade do Oeste de Santa Catarina.
authors.forename: Marlene Raimunda Andreola
authors.initials: MR
authors.lastname: Perazzoli
authors.forename: Camila Katerin
authors.initials: CK
authors.lastname: Perondi
authors.forename: Cesar Milton
authors.initials: CM
authors.lastname: Baratto
authors.forename: Evelyn
authors.initials: E
authors.lastname: Winter
authors.forename: Tânia Beatriz
authors.initials: TB
authors.lastname: Creczynski-Pasa
authors.forename: Claudriana
authors.initials: C
authors.lastname: Locatelli
mesh_terms: D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000517:prevention & control; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D058625:End Stage Liver Disease / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use*; D015870:Gene Expression; D016158:Genes, p53 / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; C008259:lauryl gallate; D005707:Gallic Acid; D002251:Carbon Tetrachloride