title: The Root Growth-Regulating Brevicompanine Natural Products Modulate the Plant Circadian Clock.
abstract: Plant growth regulating properties of brevicompanines (Brvs), natural products of the fungus Penicillium brevicompactum, have been known for several years, but further investigations into the molecular mechanism of their bioactivity have not been performed. Following chemical synthesis of brevicompanine derivatives, we studied their activity in the model plant Arabidopsis by a combination of plant growth assays, transcriptional profiling, and numerous additional bioassays. These studies demonstrated that brevicompanines cause transcriptional misregulation of core components of the circadian clock, whereas other biological read-outs were not affected. Brevicompanines thus represent promising chemical tools for investigating the regulation of the plant circadian clock. In addition, our study also illustrates the potential of an unbiased -omics-based characterization of bioactive compounds for identifying the often cryptic modes of action of small molecules.
authors.affiliation: Department of Developmental Biology, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linné-Weg 10, 50829 Köln, Germany.
authors.forename: Amaury
authors.initials: A
authors.lastname: de Montaigu
authors.affiliation: Department of Chemical Biology, Universität Duisburg-Essen, ZMB, Faculty of Biology , Universitätsstr. 2, 45117 Essen, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Oeljeklaus
authors.affiliation: Department of Chemical Biology, Universität Duisburg-Essen, ZMB, Faculty of Biology , Universitätsstr. 2, 45117 Essen, Germany.
authors.forename: Jan H
authors.initials: JH
authors.lastname: Krahn
authors.affiliation: Chemical Biology Laboratory, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linnè-Weg 10, 50829 Köln, Germany.
authors.forename: Mohamed N S
authors.initials: MNS
authors.lastname: Suliman
authors.affiliation: Department of Chemical Biology, Universität Duisburg-Essen, ZMB, Faculty of Biology , Universitätsstr. 2, 45117 Essen, Germany.|Chemical Biology Laboratory, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linnè-Weg 10, 50829 Köln, Germany.
authors.forename: Vivek
authors.initials: V
authors.lastname: Halder
authors.affiliation: Department of Developmental Biology, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linné-Weg 10, 50829 Köln, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: de Ansorena
authors.affiliation: Department of Chemical Biology, Universität Duisburg-Essen, ZMB, Faculty of Biology , Universitätsstr. 2, 45117 Essen, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Nickel
authors.affiliation: Chemical Biology Laboratory, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linnè-Weg 10, 50829 Köln, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Schlicht
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University , Šlechtitelů 241/27, 78371 Olomouc, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Plíhal
authors.affiliation: Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University , Šlechtitelů 241/27, 78371 Olomouc, Czech Republic.
authors.forename: Karolina
authors.initials: K
authors.lastname: Kubiasová
authors.affiliation: Center of Molecular Medicine, Central European Institute of Technology, Masaryk University , Kamenice 753/5, 62500 Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Radová
authors.affiliation: Bioinformatics, Department of Plant Microbe Interactions, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linnè-Weg 10, 50829 Köln, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kracher
authors.affiliation: Department of Developmental Biology, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linné-Weg 10, 50829 Köln, Germany.
authors.forename: Réka
authors.initials: R
authors.lastname: Tóth
authors.affiliation: Department of Chemical Biology, Universität Duisburg-Essen, ZMB, Faculty of Biology , Universitätsstr. 2, 45117 Essen, Germany.
authors.forename: Farnusch
authors.initials: F
authors.lastname: Kaschani
authors.affiliation: Department of Developmental Biology, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linné-Weg 10, 50829 Köln, Germany.
authors.forename: George
authors.initials: G
authors.lastname: Coupland
authors.affiliation: Chemical Biology Laboratory, Max-Planck-Institute for Plant Breeding Research , Carl-von-Linnè-Weg 10, 50829 Köln, Germany.
authors.forename: Erich
authors.initials: E
authors.lastname: Kombrink
authors.affiliation: Department of Chemical Biology, Universität Duisburg-Essen, ZMB, Faculty of Biology , Universitätsstr. 2, 45117 Essen, Germany.
authors.forename: Markus
authors.identifier: 0000-0002-6540-8520
authors.initials: M
authors.lastname: Kaiser
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology; D001688:Biological Products / Q000138:chemical synthesis / Q000494:pharmacology*; D002940:Circadian Rhythm / Q000187:drug effects*; D007211:Indoles / Q000138:chemical synthesis / Q000494:pharmacology*; D010407:Penicillium / Q000737:chemistry; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000494:pharmacology*; D018521:Plant Physiological Phenomena / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D001688:Biological Products; D007211:Indoles; D010456:Peptides, Cyclic; C499022:brevicompanine C