title: Motility of Spirochetes.
abstract: Spirochetes are bacteria distinguished by an undulate or helical cell body and intracellular flagellar called periplasmic flagella or endoflagella. Spirochetes translate by rotating the cell body. In this chapter, we show a method for simultaneous measurement of the cell body rotation and swimming speed in individual spirochete cells. We also describe a simple chemotaxis assay capable of observing the response of spirochete in real time under a microscope and quantitatively evaluating the response magnitude to attractants and repellents.
authors.affiliation: Department of Applied Physics, Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aoba-ku, Sendai, Miyagi, 980-8579, Japan. naka@bp.apph.tohoku.ac.jp.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Department of Applied Physics, Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aoba-ku, Sendai, Miyagi, 980-8579, Japan.|Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
authors.forename: Md Shafiqul
authors.initials: MS
authors.lastname: Islam
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D002633:Chemotaxis / Q000502:physiology; D005407:Flagella / Q000378:metabolism; D019897:Periplasm / Q000378:metabolism / Q000502:physiology; D012399:Rotation; D013144:Spirochaetales / Q000378:metabolism / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins
keywords: Chemotaxis; Microscopic agar-drop assay; One-sided dark-field microscope; Spirochetes