title: Bloodmeal Identification in Field-Collected Sand Flies From Casa Branca, Brazil, Using the Cytochrome b PCR Method.
abstract: PCR-based identification of vertebrate host bloodmeals has been performed on several vectors species with success. In the present study, we used a previously published PCR protocol followed by DNA sequencing based on primers designed from multiple alignments of the mitochondrial cytochrome b gene used to identify avian and mammalian hosts of various hematophagous vectors. The amplification of a fragment encoding a 359 bp sequence of the Cyt b gene yielded recognized amplification products in 192 female sand flies (53%), from a total of 362 females analyzed. In the study area of Casa Branca, Brazil, blood-engorged female sand flies such as Lutzomyia longipalpis (Lutz & Neiva, 1912), Migonemyia migonei (França, 1924), and Nyssomyia whitmani (Antunes & Coutinho, 1939) were analyzed for bloodmeal sources. The PCR-based method identified human, dog, chicken, and domestic rat blood sources.
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: G M L
authors.initials: GML
authors.lastname: Carvalho
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: F D
authors.initials: FD
authors.lastname: Rêgo
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Tanure
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: A C P
authors.initials: ACP
authors.lastname: Silva
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: T A
authors.initials: TA
authors.lastname: Dias
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: G F
authors.initials: GF
authors.lastname: Paz
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: J D
authors.initials: JD
authors.lastname: Andrade Filho
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000032:analysis*; D001717:Birds / Q000097:blood; D001938:Brazil; D045303:Cytochromes b / Q000032:analysis*; D005260:Female; D007303:Insect Vectors / Q000502:physiology*; D008322:Mammals / Q000097:blood; D024101:Mitochondrial Proteins / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011576:Psychodidae / Q000502:physiology*
chemical_list: D030161:Avian Proteins; D024101:Mitochondrial Proteins; D045303:Cytochromes b
keywords: bloodmeal identification; cytochrome b PCR; sand fly