title: Alleviation of Ultraviolet B-Induced Photodamage by Coffea arabica Extract in Human Skin Fibroblasts and Hairless Mouse Skin.
abstract: Coffea arabicaextract (CAE) containing 48.3 ± 0.4 mg/g of chlorogenic acid and a trace amount of caffeic acid was found to alleviate photoaging activity in human skin fibroblasts. In this study, polyphenol-rich CAE was investigated for its antioxidant and antiinflammatory properties, as well as for its capability to alleviate ultraviolet B (UVB)-induced photodamage in BALB/c hairless mice. The results indicated that 500 μg/mL of CAE exhibited a reducing power of 94.7%, ferrous ion chelating activity of 46.4%, and hydroxyl radical scavenging activity of 20.3%. The CAE dose dependently reduced UVB-induced reactive oxygen species (ROS) generation in fibroblasts. Furthermore, CAE inhibited the UVB-induced expression of cyclooxygenase-2 andp-inhibitor κB, and the translocation of nuclear factor-kappa B (NF-κB) to the nucleus of fibroblasts. In addition, CAE alleviated UVB-induced photoaging and photodamage in BALB/c hairless mice by restoring the collagen content and reduced UVB-induced epidermal hyperplasia. CAE also inhibited UVB-induced NF-κB, interleukin-6, and matrix metalloproteinase-1 expression in the hairless mouse skin. The results indicated that CAE exhibits antiphotodamage activity by inhibiting UV-induced oxidative stress and inflammation. Therefore, CAE is a candidate for use in antioxidant, antiinflammatory, and antiphotodamage products.
authors.affiliation: Department of Dermatology, China Medical University Hospital, Taichung 40402, Taiwan. wu.poyuan@gmail.com.|School of Medicine, China Medical University, Taichung 404, Taiwan. wu.poyuan@gmail.com.
authors.forename: Po-Yuan
authors.initials: PY
authors.lastname: Wu
authors.affiliation: Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. john5523@ntsu.edu.tw.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. dooo517@outlook.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. hedy9088723@hotmail.com.
authors.forename: Ya-Han
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. a50535l@hotmail.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. lesley456987@yahoo.com.tw.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. kcwen0520@mail.cmu.edu.tw.
authors.forename: Kuo-Ching
authors.initials: KC
authors.lastname: Wen
authors.affiliation: Department of Biotechnology, Asia University, Taichung 41354, Taiwan. yihlin@asia.edu.tw.
authors.forename: Chien-Yih
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Sports Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. meichich@kmu.edu.tw.
authors.forename: Mei-Chich
authors.initials: MC
authors.lastname: Hsu
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan. hmchiang@mail.cmu.eu.tw.
authors.forename: Hsiu-Mei
authors.initials: HM
authors.lastname: Chiang
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D002478:Cells, Cultured; D040503:Coffea / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D059808:Polyphenols / Q000008:administration & dosage* / Q000494:pharmacology; D011855:Radiodermatitis / Q000378:metabolism / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species
keywords: Coffea arabica; antioxidant; inflammatory; inhibitor κB (IκB); nuclear factor-kappa B (NF-κB); photodamage