title: Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis.
abstract: The mammalian epididymis is an exceptionally long ductal system tasked with the provision of one of the most complex intraluminal fluids found in any exocrine gland. This specialized milieu is continuously modified by the combined secretory and absorptive of the surrounding epithelium and thus finely tuned for its essential roles in promoting sperm maturation and storage. While considerable effort has been focused on defining the composition of the epididymal fluid, relatively less is known about the intracellular trafficking machinery that regulates this luminal environment. Here, we characterize the ontogeny of expression of a master regulator of this machinery, the dynamin family of mechanoenzymes. Our data show that canonical dynamin isoforms were abundantly expressed in the juvenile mouse epididymis. However, in peripubertal and adult animals dynamin takes on a heterogeneous pattern of expression such that the different isoforms displayed both cell- and segment-specific localization. Thus, dynamin 1 and 3 were predominately localized in the distal epididymal segments (corpus and cauda), where they were found within clear and principal cells, respectively. In contrast, dynamin 2 was expressed throughout the epididymis, but localized to the Golgi apparatus of the principal cells in the proximal (caput) segment and the luminal border of these cells in more distal segments. These dynamin isoforms are therefore ideally positioned to play complementary, nonredundant roles in the regulation of the epididymal milieu. In support of this hypothesis, selective inhibition of dynamin altered the profile of proteins secreted from an immortalized caput epididymal cell line.
authors.affiliation: Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Geoffry N
authors.initials: GN
authors.lastname: De Iuliis
authors.affiliation: School of Biological Sciences, University of Auckland, Auckland, New Zealand.
authors.forename: Adrian P
authors.initials: AP
authors.lastname: Turner
authors.affiliation: Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Reid
authors.affiliation: Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Anderson
authors.affiliation: Priority Research Centre for Chemical Biology, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: McCluskey
authors.affiliation: Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.|School of Biological Sciences, University of Auckland, Auckland, New Zealand.|Priority Research Centre for Chemical Biology, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Eileen A
authors.initials: EA
authors.lastname: McLaughlin
authors.affiliation: Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
authors.forename: Brett
authors.initials: B
authors.lastname: Nixon
mesh_terms: D000818:Animals; D034281:Dynamins / Q000378:metabolism*; D004822:Epididymis / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051379:Mice; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism
chemical_list: C480628:Atp6v1b1 protein, mouse; D025262:Vacuolar Proton-Translocating ATPases; D034281:Dynamins
keywords: epididymal milieu, protein trafficking, exosomes,
                      epididymosomes, apocrine secretion, merocrine secretion; epididymis, sperm maturation, dynamin