title: Notoginsenoside R1 suppresses wear particle-induced osteolysis and RANKL mediated osteoclastogenesis in vivo and in vitro.
abstract: Aseptic periprosthetic osteolysis is a complication induced by wear particles after hip joint replacement, which shortens the service life of prostheses. Although many methods have been applied to prevent this complication, the results were not always successful. In the present study, we explored the prophylactic effect of notoginsenoside R1 (NG-R1) on a Ti particle-induced mouse calvarial osteolysis model and the inhibitory effect on RANKL-mediated osteoclastogenesis and osteoclast bone resorption. Our results indicated that intraperitoneal injection of NG-R1(10 or 20mg/kg/day), a constituent of notoginseng, alleviates Ti particle-induced mouse calvarial osteolysis after twelve days post-treatment. In vitro, we observed that NG-R1 inhibits RANKL-mediated mitogen-activated protein kinases (MAPKs), including the phosphorylation levels of JNK1/2, P38, ERK1/2 and NF-kappa B (NF-κB) signalling pathways, and suppresses RANKL-mediated osteoclastogenesis and osteoclast bone resorption. These results suggest that NG-R1 may be a promising therapeutic agent for preventing wear particle-induced periprosthetic osteolysis.
authors.affiliation: Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China. Electronic address: zhaoshuai19870624@163.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China. Electronic address: yanlianqi@126.com.
authors.forename: Lianqi
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China. Electronic address: doclee86@163.com.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Li
authors.affiliation: Dalian Medical University, Dalian 116044, Liaoning, China. Electronic address: 1395637072@qq.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China. Electronic address: docyusun@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou 225001, China. Electronic address: jingchengwyz@163.com.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D019643:Arthroplasty, Replacement; D001862:Bone Resorption / Q000517:prevention & control*; D004195:Disease Models, Animal; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D036145:Ginsenosides / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D010010:Osteoclasts / Q000187:drug effects*; D010014:Osteolysis / Q000517:prevention & control*; D011183:Postoperative Complications / Q000517:prevention & control*; D011475:Prosthesis Failure / Q000009:adverse effects; D053245:RANK Ligand / Q000378:metabolism; D000067996:RAW 264.7 Cells; D015398:Signal Transduction; D014025:Titanium
chemical_list: D036145:Ginsenosides; D016328:NF-kappa B; D053245:RANK Ligand; D014025:Titanium; D048049:Extracellular Signal-Regulated MAP Kinases; C072936:notoginsenoside R1
keywords: Aseptic prosthesis loosening; Mitogen-activated protein kinase; NF-kappa B; Notoginsenoside R1; Osteoclast; Osteolysis