title: MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells.
abstract: MicroRNAs regulate insulin secretion, pancreatic development and beta cell differentiation. However, the function of microRNAs in the formation of insulin-producing cells (IPCs) from adult stem cells is poorly understood. We examine the microRNA expression profile in nestin-positive umbilical cord-derived mesenchymal stem cells (N-UCMSCs) and nestin-positive pancreatic mesenchymal stem cells using a deep sequencing approach. We also selected specific microRNAs for overexpression in N-UCMSCs and found that miR-375 and miR-26a induced IPCs differentiation from N-UCMSCs by downregulating target genes including mtpn, sox6, bhlhe22 and ccnd1. Small interfering RNAs were also used to knock down these genes in N-UCMSCs to induce the formation of IPCs. These results suggest that endogenous microRNAs involved in the formation of IPCs from adult stem cells show promise for advancing the development of an effective cell transplant therapy for diabetes. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Bai
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xiangchen
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Kunfu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Shan
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D002645:Chickens; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000637:transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D016513:Mice, SCID; D035683:MicroRNAs / Q000378:metabolism*; D064231:Nestin / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D035683:MicroRNAs; D064231:Nestin; D034741:RNA, Small Interfering
keywords: insulin secretion; insulin-producing cells; mesenchymal stem cells; microRNAs; small interfering RNAs