title: Genome editing: a robust technology for human stem cells.
abstract: Human pluripotent stem cells comprise induced pluripotent and embryonic stem cells, which have tremendous potential for biological and therapeutic applications. The development of efficient technologies for the targeted genome alteration of stem cells in disease models is a prerequisite for utilizing stem cells to their full potential. Genome editing of stem cells is possible with the help of synthetic nucleases that facilitate site-specific modification of a gene of interest. Recent advances in genome editing techniques have improved the efficiency and speed of the development of stem cells for human disease models. Zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system are powerful tools for editing DNA at specific loci. Here, we discuss recent technological advances in genome editing with site-specific nucleases in human stem cells.
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea.
authors.forename: Arun Pandian
authors.initials: AP
authors.lastname: Chandrasekaran
authors.affiliation: Division of Bioindustry, Department of Food Biotechnology, College of Medical and Life Science, Silla University, Seoul, Republic of Korea. songmj@silla.ac.kr.
authors.forename: Minjung
authors.initials: M
authors.lastname: Song
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea. suri28@hanyang.ac.kr.|College of Medicine, Hanyang University, Seoul, Republic of Korea. suri28@hanyang.ac.kr.
authors.forename: Suresh
authors.identifier: 0000-0002-4038-1085
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics / Q000378:metabolism; D064113:CRISPR-Cas Systems / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000378:metabolism; D000072669:Gene Editing; D015894:Genome, Human; D006801:Humans; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D000069896:Transcription Activator-Like Effector Nucleases / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D019713:Receptors, CCR5; D016159:Tumor Suppressor Protein p53; D004706:Endodeoxyribonucleases; D000069896:Transcription Activator-Like Effector Nucleases; D018631:ras Proteins
keywords: Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system; Embryonic stem cells; Induced pluripotent stem cells; Transcription activator-like effector nucleases; Zinc finger nucleases