title: Systematic comparison of biologically active foreign ions-codoped calcium phosphate microparticles on osteogenic differentiation in rat osteoporotic and normal mesenchymal stem cells.
abstract: Osteoporosis is a disease characterized by structural deterioration of bone tissue, leading to skeletal fragility with increased fracture risk. Calcium phosphates (CaPs) are widely used in bone tissue engineering strategies as they have similarities to bone apatite except for the absence of trace elements (TEs) in the CaPs. Bioactive glasses (BGs) have also been used successfully in clinic for craniomaxillofacial and dental applications during the last two decades due to their excellent potential for bonding with bone and inducing osteoblastic differentiation. In this study, we evaluated the osteogenic effects of the ionic dissolution products of the quaternary Si-Sr-Zn-Mg-codoped CaP (TEs-CaP) or 45S5 Bioglass® (45S5 BG), both as mixtures and separately, on rat bone marrow-derived mesenchymal stem cells (rOMSCs & rMSCs) from osteoporotic and normal animals, using an MTT test and Alizarin Red S staining. The materials enhanced cell proliferation and osteogenic differentiation, especially the combination of the BG and TEs-CaP. Analysis by quantitative PCR and ELISA indicated that the expression of osteogenic-specific genes and proteins were elevated. These investigations suggest that the TEs-CaP and 45S5 BG operate synergistically to create an extracellular environment that promotes proliferation and terminal osteogenic differentiation of both osteoporotic and normal rMSCs.
authors.affiliation: Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital, School of Medicine of Zhejiang University, Hangzhou 310009, China.
authors.forename: San-Zhong
authors.initials: SZ
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital, School of Medicine of Zhejiang University, Hangzhou 310009, China.
authors.forename: Xuan-Wei
authors.initials: XW
authors.lastname: Wang
authors.affiliation: Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xian-Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.affiliation: Rui'an People's Hospital & The 3rd Affiliated Hospital to Wenzhou Medical University, Rui'an 325005, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Rui'an People's Hospital & The 3rd Affiliated Hospital to Wenzhou Medical University, Rui'an 325005, China.
authors.forename: Guo-Jing
authors.initials: GJ
authors.lastname: Yang
authors.affiliation: Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Lin-Hong
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Dental and Craniofacial Research Institute, School of Dentistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Xin-Li
authors.initials: XL
authors.lastname: Zhang
authors.affiliation: Dental and Craniofacial Research Institute, School of Dentistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Kang
authors.initials: K
authors.lastname: Ting
authors.affiliation: Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chang-You
authors.initials: CY
authors.lastname: Gao
authors.affiliation: Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Xiao-Zhou
authors.initials: XZ
authors.lastname: Mou
authors.affiliation: Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhong-Ru
authors.initials: ZR
authors.lastname: Gou
authors.affiliation: Department of Cardiology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zou
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007477:Ions / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D010024:Osteoporosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051381:Rats
chemical_list: D002130:Calcium Phosphates; D007477:Ions; C020243:calcium phosphate; D000469:Alkaline Phosphatase
keywords: microparticles; mineral micronutrients; osteogenic differentiation; rMSCs; rOMSCs