title: Comparison of MastBurkholderia Cepacia, Ashdown + Gentamicin, andBurkholderia PseudomalleiSelective Agar for the Selective Growth ofBurkholderiaSpp.
abstract: Reliable identification of pathogenicBurkholderiaspp. likeBurkholderia malleiandBurkholderia pseudomalleiin clinical samples is desirable. Three different selective media were assessed for reliability and selectivity with variousBurkholderiaspp. and nontarget organisms. MastBurkholderia cepaciaagar, Ashdown + gentamicin agar, andB. pseudomalleiselective agar were compared. A panel of 116 reference strains and well-characterized clinical isolates, comprising 30B. pseudomallei, 20B. mallei, 18 otherBurkholderiaspp., and 48 nontarget organisms, was used for this assessment. While allB. pseudomalleistrains grew on all three tested selective agars, the otherBurkholderiaspp. showed a diverse growth pattern. Nontarget organisms, i.e., nonfermentative rod-shaped bacteria, other species, and yeasts, grew on all selective agars. Colony morphology did not allow unambiguous discrimination. While the assessed selective media reliably allowed the growth of a wide range ofB. pseudomalleistrains, growth of otherBurkholderiaspp. is only partially ensured. Growth of various nontarget organisms has to be considered. Therefore, the assessed media can only be used in combination with other confirmative tests in the diagnostic procedure for the screening for melioidosis or glanders.
authors.affiliation: Department of Dermatology, German Armed Forces Hospital of Hamburg , Hamburg, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Edler
authors.affiliation: CBRN Defence, Safety and Environmental Protection School, Science Division.
authors.forename: Henri
authors.initials: H
authors.lastname: Derschum
authors.affiliation: Bundeswehr Medical Academy , Munich, Germany.
authors.forename: Mirko
authors.initials: M
authors.lastname: Köhler
authors.affiliation: Friedrich Loeffler Institute, Federal Research Institute for Animal Health , Jena, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
authors.affiliation: Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany; Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany.
authors.forename: Hagen
authors.initials: H
authors.lastname: Frickmann
authors.affiliation: Department of Preventive Medicine, Bundeswehr Medical Academy , Munich, Germany.
authors.forename: Ralf Matthias
authors.initials: RM
authors.lastname: Hagen
keywords: Burkholderia mallei; Burkholderia pseudomallei; Burkholderia spp; comparison; selective agar