title: 5-Aminolevulinic acid production from inexpensive glucose by engineering the C4 pathway in Escherichia coli.
abstract: 5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L. Following optimization of biosynthesis pathways towards coenzyme A and precursor (glycine and succinyl-CoA), and downregulation of hemB expression, the production of ALA was further increased to 2.81 g/L in batch-fermentation.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Huanjiao
authors.initials: H
authors.lastname: Weng
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China. zkang@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China. zkang@jiangnan.edu.cn.|The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China. zkang@jiangnan.edu.cn.
authors.forename: Zhen
authors.identifier: 0000-0003-1479-3075
authors.initials: Z
authors.lastname: Kang
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000235:genetics / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D011163:Hydroxymethylbilane Synthase / Q000235:genetics / Q000378:metabolism; D007218:Industrial Microbiology; D000074041:Microorganisms, Genetically-Modified / Q000235:genetics*; D016354:Rhodobacter capsulatus / Q000235:genetics / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D000622:Aminolevulinic Acid; C012046:succinyl-coenzyme A; D000624:5-Aminolevulinate Synthetase; D011163:Hydroxymethylbilane Synthase; C073160:hemC protein, E coli; D005947:Glucose; D005998:Glycine
keywords: 5-Aminolevulinic acid; Coenzyme A; HemB; Metabolic engineering; The C4 pathway