title: Chemically different non-thermal plasmas target distinct cell death pathways.
abstract: A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.
authors.affiliation: Institute of Physics of the Academy of Sciences of the Czech Republic, Prague, 18221, Czech Republic. lunov@fzu.cz.
authors.forename: Oleg
authors.initials: O
authors.lastname: Lunov
authors.affiliation: Institute of Physics of the Academy of Sciences of the Czech Republic, Prague, 18221, Czech Republic.
authors.forename: Vitalii
authors.initials: V
authors.lastname: Zablotskii
authors.affiliation: Institute of Physics of the Academy of Sciences of the Czech Republic, Prague, 18221, Czech Republic.
authors.forename: Olexander
authors.initials: O
authors.lastname: Churpita
authors.affiliation: Institute for Clinical & Experimental Medicine (IKEM), Prague, 14021, Czech Republic.
authors.forename: Mariia
authors.initials: M
authors.lastname: Lunova
authors.affiliation: Institute for Clinical & Experimental Medicine (IKEM), Prague, 14021, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Jirsa
authors.affiliation: Institute of Physics of the Academy of Sciences of the Czech Republic, Prague, 18221, Czech Republic.
authors.forename: Alexandr
authors.identifier: http://orcid.org/0000-0002-5116-8781
authors.initials: A
authors.lastname: Dejneka
authors.affiliation: Institute of Physics of the Academy of Sciences of the Czech Republic, Prague, 18221, Czech Republic.|Institute of Experimental Medicine AS CR, Prague, 14220, Czech Republic.
authors.forename: Šárka
authors.initials: Š
authors.lastname: Kubinová
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects; D006371:Helium / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009336:Necrosis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D058626:Plasma Gases / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D006371:Helium