title: Effects of aflatoxin B1 on mitochondrial respiration, ROS generation and apoptosis in broiler cardiomyocytes.
abstract: Aflatoxin B1 (AFB1) develops various toxic effects in the liver by impairing mitochondrial function, inducing cell apoptosis. However, little is focused on its toxicity to broiler cardiomyocytes (BCMs). Here, the mitochondrial membrane potential (MMP), reactive oxygen species (ROS) generation, cardiac troponin T (cTnT) location, apoptosis induced by AFB1, and antioxidative genes were investigated in BCMs. It was found that AFB1 evoked intracellular ROS generation, and induced apoptosis in BCMs. AFB1 treatment resulted in increased percentage of apoptotic cells, increased location range of cTnT in cytoplasm, upregulated messenger RNA (mRNA) expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and downregulated mRNA expressions of Mn-superoxide dismutase in BCMs. These findings suggested AFB1 treatment caused significant cardiomyocyte damage and cardiotoxicity, impairment of mitochondrial functions, activated ROS generation, and induced apoptosis, and probably was involved in the Nrf2 signal pathway in BCMs.
authors.affiliation: College of Life Sciences, South-Central University for Nationalities, Wuhan, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Wang
authors.affiliation: The People's Hospital of Hanchuan City, Hanchuan, China.
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Xu
authors.affiliation: College of Life Sciences, South-Central University for Nationalities, Wuhan, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yu
authors.affiliation: The People's Hospital of Hanchuan City, Hanchuan, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Xu
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D002645:Chickens; D003593:Cytoplasm / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D015870:Gene Expression; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism* / Q000473:pathology*; D050983:NF-E2 Transcription Factor / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D020107:Troponin T / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D050983:NF-E2 Transcription Factor; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D020107:Troponin T; D016604:Aflatoxin B1; D013482:Superoxide Dismutase
keywords: aflatoxin B1; apoptosis; broiler cardiomyocytes; cardiac troponin T; reactive oxygen species