title: Identification and functional analyses of novel antioxidant peptides and antimicrobial peptides from skin secretions of four East Asian frog species.
abstract: In the present study, we identified 50 peptides that are classified into 21 peptide families with antioxidant and/or antimicrobial activity from Amolops daiyunensis, Pelophylax hubeiensis, Hylarana maosuoensis and Nanorana pleskei, which belong to four different genera in the Ranidae and Dicroglossidae families. These four frog species were found for the first time to express antioxidant peptides (AOPs) and antimicrobial peptides (AMPs). These peptides include seven newly discovered families daiyunin-1, daiyunin-2, daiyunin-3, maosonensis-1MS1, pleskein-1, pleskein-2, and pleskein-3. Antioxidant and antimicrobial activity assays showed that some of these peptides have good biological activities. For example, at a concentration of 50 μM, nigroain-B-MS1, and nigroain-C-MS1 both exhibited relatively strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (ABTS) free radical scavenging ability, with eradication rates of 99.7% and 68.3% (nigroain-B-MS1), and 99.8% and 58.3% (nigroain-C-MS1), respectively. These peptides are potential candidates for the development of novel antioxidant or AMP preparations.
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.|The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Ren
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Baowen
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Sihan
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Basic, Tianjin Agricultural University, Tianjin 300384, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ren
authors.affiliation: Instrumental Analysis Center, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction / Q000187:drug effects; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011898:Ranidae / Q000145:classification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010455:Peptides
keywords: amphibia; antimicrobial peptide; antioxidant peptide; free radical; skin