title: Detection of Brucella canis infection in dogs by blood culture and bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. Organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The instrument-provided security library identified the isolates as Brucella species. The isolates were further identified as B. canis with the help of phenotypic and genotypic characteristics. The mass spectral profiles from characterized B. canis isolates, when added to the MALDI-TOF MS standard reference library, allowed successful presumptive identification of B. canis.
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).|Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
authors.forename: Tanya J
authors.initials: TJ
authors.lastname: Purvis
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).|Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
authors.forename: Donna
authors.initials: D
authors.lastname: Krouse
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).|Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
authors.forename: Dawn
authors.initials: D
authors.lastname: Miller
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).|Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
authors.forename: Julia
authors.initials: J
authors.lastname: Livengood
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).|Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
authors.forename: Nagaraja R
authors.initials: NR
authors.lastname: Thirumalapura
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Manhattan, KS (Purvis).|Pennsylvania Veterinary Laboratory, Pennsylvania Department of Agriculture, Harrisburg, PA (Krouse, Miller, Livengood, Thirumalapura, Tewari).
authors.forename: Deepanker
authors.initials: D
authors.lastname: Tewari
mesh_terms: D000818:Animals; D000071997:Blood Culture / Q000379:methods / Q000662:veterinary*; D041002:Brucella canis / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000662:veterinary*
keywords: Blood culture; Brucella canis; dogs; lysis; mass spectrometry