title: Investigation of Genetic Diversity ofFusarium oxysporumf. sp.fragariaeUsing PCR-RFLP.
abstract: Fusarium wilts of strawberry, caused byFusarium oxysporumf. sp.fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-knownF. oxysporumhave different genetic information fromF. oxysporumf. sp.fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed usingF. oxysporumf. sp.fragariaespecies-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-α, and β-tubulin genes of the pathogens and four restriction enzymes:AluI,HhaI,HinP1I andHpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the β-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region.
authors.affiliation: Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Agricultural Plant Science and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Nam Jun
authors.initials: NJ
authors.lastname: Kang
authors.affiliation: Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Youn-Sig
authors.initials: YS
authors.lastname: Kwak
authors.affiliation: R&D Coordination Division, Rural Development Administration, Wanju 55365, Korea.
authors.forename: Choungkeun
authors.initials: C
authors.lastname: Lee
keywords: Fusarium wilt; PCR-RFLP analysis; strawberry