title: Scaffold of Selenium Nanovectors and Honey Phytochemicals for Inhibition ofPseudomonas aeruginosaQuorum Sensing and Biofilm Formation.
abstract: Honey is an excellent source of polyphenolic compounds that are effective in attenuating quorum sensing (QS), a chemical process of cell-to-cell communication system used by the opportunistic pathogenPseudomonas aeruginosato regulate virulence and biofilm formation. However, lower water solubility and inadequate bioavailability remains major concerns of these therapeutic polyphenols. Its therapeutic index can be improved by using nano-carrier systems to target QS signaling potently. In the present study, we fabricated a unique drug delivery system comprising selenium nanoparticles (SeNPs; non-viral vectors) and polyphenols of honey (HP) for enhancement of anti-QS activity of HP againstP. aeruginosaPAO1. The developed selenium nano-scaffold showed superior anti-QS activity, anti-biofilm efficacy, and anti-virulence potential in bothin-vitroandin-vivoover its individual components, SeNPs and HP. LasR is inhibited by selenium nano-scaffoldin-vitro. Using computational molecular docking studies, we have also demonstrated that the anti-virulence activity of selenium nano-scaffold is reliant on molecular binding that occurs between HP and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Our preliminary investigations with selenium-based nano-carriers hold significant promise to improve anti-virulence effectiveness of phytochemicals by enhancing effective intracellular delivery.
authors.affiliation: Pharmacognosy and Ethnopharmacology Division, Herbal Nanobiotechnology Lab, CSIR-National Botanical Research Institute Lucknow, India.
authors.lastname: Prateeksha
authors.affiliation: Centre of Excellence in Materials Science (Nanomaterials), Z. H. College of Engineering and Technology, Aligarh Muslim UniversityAligarh, India; TERI-Deakin Nanobiotechnology Centre, The Energy Research InstituteNew Delhi, India.
authors.forename: Braj R
authors.initials: BR
authors.lastname: Singh
authors.affiliation: Centre of Excellence in Materials Science (Nanomaterials), Z. H. College of Engineering and Technology, Aligarh Muslim UniversityAligarh, India; TERI-Deakin Nanobiotechnology Centre, The Energy Research InstituteNew Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Shoeb
authors.affiliation: Pharmacognosy and Ethnopharmacology Division, Herbal Nanobiotechnology Lab, CSIR-National Botanical Research Institute Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Centre of Excellence in Materials Science (Nanomaterials), Z. H. College of Engineering and Technology, Aligarh Muslim UniversityAligarh, India; TERI-Deakin Nanobiotechnology Centre, The Energy Research InstituteNew Delhi, India.
authors.forename: A H
authors.initials: AH
authors.lastname: Naqvi
authors.affiliation: Molecular Glyco-Biotechnology Group, Discipline of Biochemistry, School of Natural Sciences, NUI GalwayGalway, Ireland; Department of Chemistry and Biotechnology, ERA Chair of Green Chemistry, School of Science, Tallinn University of TechnologyTallinn, Estonia.
authors.forename: Vijai K
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Pharmacognosy and Ethnopharmacology Division, Herbal Nanobiotechnology Lab, CSIR-National Botanical Research Institute Lucknow, India.
authors.forename: Brahma N
authors.initials: BN
authors.lastname: Singh
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D006722:Honey; D051379:Mice; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008968:Molecular Conformation; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D064209:Phytochemicals / Q000737:chemistry* / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding; D011552:Pseudomonas Infections / Q000382:microbiology / Q000401:mortality; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*; D053038:Quorum Sensing / Q000187:drug effects*; D012643:Selenium; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism; D014774:Virulence / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C080365:LasR protein, Pseudomonas aeruginosa; D064209:Phytochemicals; D059808:Polyphenols; D015534:Trans-Activators; D012643:Selenium
keywords: Pseudomonas aeruginosa; honey; nanovectors; polyphenols; quorum sensing; selenium