title: Nano-encapsulant of ascorbic acid-loaded apoferritin-assisted photoelectrochemical sensor for protease detection.
abstract: A signal-on photoelectrochemical (PEC) biosensor based on nano-encapsulant of ascorbic acid-loaded apoferritin-assisted for protease detection is described. The loaded ascorbic acid could be released from the central cavity of apoferritin into the buffer solution as sacrificial electron donor to capture the photo-generated holes of CdTe quantum dots when light is turned on. In this system, the biosensor relied on monitoring the photocurrent intensity as a result of enzymatic substrate proteolysis in the homogeneous aqueous solution. A low detection limit of 2.7ngmL-1for trypsin in the linear range from 30 to 450ngmL-1is achieved. We believe that such a sensing system not only holds the potential ability as a probe for trypsin activity assay, but also could be used for the corresponding inhibitor-screening. It might provide a potentially feasible alternative tool for determining trypsin in human serum in a clinical laboratory. The established method could open a different perspective for PEC enzyme detection and provide a new platform for future development of PEC analysis with other clinically important proteins.
authors.affiliation: Anhui Key Laboratory of Chem-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China; Departement of Chemistry, Wannan Medical College, Wuhu 241002, PR China.
authors.forename: Jiexia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Anhui Key Laboratory of Chem-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: gczhao@mail.ahnu.edu.cn.
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Zhao
mesh_terms: D001052:Apoferritins / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D055668:Photochemical Processes; D045663:Quantum Dots; D014357:Trypsin / Q000032:analysis*
chemical_list: D001052:Apoferritins; D014357:Trypsin; D001205:Ascorbic Acid
keywords: Apoferritin; Photoelectrochemistry; Protease; Trypsin sensing