title: Identification and in vitro antioxidant activities of phenolic compounds isolated from Cynoglossum cheirifolium L.
abstract: In an extensive search for bioactive compounds from plant sources, the quantitative and qualitative characterisation of the compounds present in Cynoglossum cheirifolium extracts was studied. Total phenolic and flavonoid contents were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined through DPPH•scavenging activity and Ferric reducing antioxidant power (FRAP). Our study showed that leaves produce more phenolic compounds, followed by flowering aerial part. The butanolic fraction obtained from leaves extract exhibited the highest total phenolics (78.65 ± 3.58 mg GAE/g DW) and flavonoids (22.15 ± 4.66 mg CE/g DW). In contrast, this fraction displayed the highest DPPH•scavenging ability with IC50values of 0.06 ± 0.003 mg/mL. The RP-HPLC-PDA analysis of phenolic compounds from leaves of C. cheirifolium lets to identify: rosmarinic acid, ferulic acid, caffeic acid, p-coumaric acid, syringic acid, sinapic acid and rutin. The obtained results indicate that this plant represent a valuable source of natural antioxidants.
authors.affiliation: a Faculté SNV-STU, Laboratoire des Produits naturels, Département de Biologie , Université Aboubekr BELKAÏD Tlemcen , Imama , Algeria.
authors.forename: Bensaid
authors.initials: B
authors.lastname: Ilhem
authors.affiliation: a Faculté SNV-STU, Laboratoire des Produits naturels, Département de Biologie , Université Aboubekr BELKAÏD Tlemcen , Imama , Algeria.
authors.forename: Atik Bekkara
authors.initials: AB
authors.lastname: Fawzia
authors.affiliation: b Centre de Recherche scientifique et technique en Analyses Physico-chimiques (CRAPC) , Bou-Ismail , Algeria.
authors.forename: El Haci
authors.initials: EH
authors.lastname: Imad Abdelhamid
authors.affiliation: a Faculté SNV-STU, Laboratoire des Produits naturels, Département de Biologie , Université Aboubekr BELKAÏD Tlemcen , Imama , Algeria.
authors.forename: Belarbi
authors.initials: B
authors.lastname: Karima
authors.affiliation: c Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene USTHB , Bab-Ezzouar , Algeria.
authors.forename: Beddou
authors.initials: B
authors.lastname: Fawzia
authors.affiliation: a Faculté SNV-STU, Laboratoire des Produits naturels, Département de Biologie , Université Aboubekr BELKAÏD Tlemcen , Imama , Algeria.
authors.forename: Bekhechi
authors.initials: B
authors.lastname: Chahrazed
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D028082:Boraginaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D005419:Flavonoids; D005707:Gallic Acid; D010636:Phenols; D010936:Plant Extracts; D000097123:Rosmarinic Acid; C001945:syringic acid
keywords: Cynoglossum cheirifolium L.; RP-HPLC-PDA; antioxidant activity; flavonoids; phenolics