title: Fraxin Prevents Chemically Induced Hepatotoxicity by Reducing Oxidative Stress.
abstract: Fraxin isolated fromAcer tegmentosumis reported to exert potent anti-oxidative stress action. However, pharmacological activities of fraxin remain to be elucidated. This study investigated the potential hepatoprotective effects of fraxin and the underlying signaling mechanism involved. Treatment with fraxin significantly lowered the serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in a CCl₄-induced hepatotoxicity rat model. In the fraxin-treated group, glutathione (GSH) significantly increased, while the malondialdehyde (MDA) in the liver significantly decreased. Fraxin also showed radical-scavenging activity. Furthermore, it significantly reduced the t-BHP-induced cytotoxicity and production of reactive oxygen species (ROS) in Hep G2. Fraxin protected Hep G2 cells through Nrf2 pathway-dependent HO-1 expression. The results of this study indicate that fraxin shows potent hepatoprotective effects in vitro and in vivo, presumably through direct antioxidant activity and the Nrf2-mediated antioxidant enzyme system.
authors.affiliation: Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Korea. oama611@nate.com.
authors.forename: Bo Yoon
authors.initials: BY
authors.lastname: Chang
authors.affiliation: College of Pharmacy, Pusan National University, San 30, Jangjeon-dong, Busan 46241, Korea. youngjung@pusan.ac.kr.
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Jung
authors.affiliation: Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Korea. ycs1991@naver.com.
authors.forename: Chi-Su
authors.initials: CS
authors.lastname: Yoon
authors.affiliation: Dongbu Eastern Herbal Medicine Agricultural Association Corporation, Yeosunro 1679, Sunchun-si, Jeonnam 58019, Korea. 5811684@daum.net.
authors.forename: Jun Seok
authors.initials: JS
authors.lastname: Oh
authors.affiliation: Dongbu Eastern Herbal Medicine Agricultural Association Corporation, Yeosunro 1679, Sunchun-si, Jeonnam 58019, Korea. 5811684@naver.com.
authors.forename: Jae Heoi
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Korea. wku114@naver.com.
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Korea. sungykim@wonkwang.ac.kr.
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001706:Biopsy; D002251:Carbon Tetrachloride / Q000009:adverse effects; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003374:Coumarins; D051267:NF-E2-Related Factor 2; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D002251:Carbon Tetrachloride; D051547:Heme Oxygenase-1; C080614:fraxin
keywords: A. tegmentosum; HO-1; Nrf2; antioxidant; fraxin; hepatoprotective