title: Quantitative Proteomics Analysis of Developmental Reprogramming in Protoplasts of the Moss Physcomitrella patens.
abstract: The moss Physcomitrella patens is a model system for studying Plant developmental processes. To better understand the biochemical and physiological changes involved in developmental reprogramming, we conducted a quantitative proteomics analysis for protonemata, protoplasts made therefrom and protoplasts regenerated for 2 d. Using an iTRAQ peptide labeling strategy and liquid chromatography-tandem mass spectrometry (LC-MS/MS), >3,000 peptides and 1,000 proteins were quantified. Of these, 162 proteins were identified as having differential abundances during developmental reprogramming. These proteins were involved in various biological functions, such as defense, energy production, translation, metabolism, protein destination and storage, transcription, transport, cell growth/division, cell structure and signal transduction. Of these, the proteins involved in energy production and translation increased in abundance, while many of the metabolism and defense proteins decreased in abundance. In addition, most of the cell growth/division, protein stability and cell structure proteins were also down-regulated. This is the first report on the metabolic changes involved in developmental reprogramming in protoplasts. The significance of metabolic networks in developmental programming is beginning to emerge. Our study suggested that stress signals, energy metabolism and ribosomal proteins are pivotal components during developmental programming.
authors.affiliation: Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, Beijing University of Agriculture, Beijing, China.|Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit trees, Beijing University of Agriculture, Beijing, China.|College of Life Sciences, Capital Normal University, Beijing, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, Beijing University of Agriculture, Beijing, China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Yang
authors.affiliation: Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, Beijing University of Agriculture, Beijing, China.
authors.forename: Chunya
authors.initials: C
authors.lastname: Bu
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing, China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: He
mesh_terms: D019068:Bryopsida / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011523:Protoplasts / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D010940:Plant Proteins
keywords: Developmental reprogramming; Physcomitrella patens; Protoplast; Quantitative proteome