title: Tethered bone morphogenetic protein-2 onto sulfonated-polyrotaxane based surfaces promotes osteogenic differentiation of MC3T3-E1 cells.
abstract: Sulfonated-polyrotaxane (PRX)-coated surfaces with tethered bone morphogenetic protein-2 (BMP-2) were designed for inducing osteogenic differentiation of MC3T3-E1 cells (preosteoblasts). Sulfonated-PRX triblock copolymers composed of sulfopropyl ether-modified α-cyclodextrins (α-CDs) threaded onto a poly(ethylene glycol) (PEG) chain as a PRX segment and poly(benzyl methacrylate) (PBzMA) at both terminals of the PEG as anchoring segments were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. BMP-2 tethering on the PRX surfaces with a large number of threaded α-CDs was suitable for the proliferation of MC3T3-E1 cells, regardless of the presence or absence of sulfopropyl groups. However, the differentiation of MC3T3-E1 cells according to mineralization assays and the gene expression levels of runt-related transcription factor 2, alkaline phosphatase, and osteocalcin was markedly enhanced by BMP-2 tethering on the sulfonated-PRX surface. These results suggest that sulfonated-PRX surfaces can effectively promote the proliferation and induce the osteogenic differentiation of MC3T3-E1 cells by the number of threaded α-CDs and tethered-BMP-2 using electrostatic interactions with sulfopropyl groups.
authors.affiliation: a Department of Organic Biomaterials , Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , Tokyo , Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Arisaka
authors.affiliation: a Department of Organic Biomaterials , Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , Tokyo , Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yui
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D003505:Cyclodextrins / Q000737:chemistry* / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D020442:Poloxamer / Q000737:chemistry* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043862:Rotaxanes / Q000737:chemistry* / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D003505:Cyclodextrins; D012333:RNA, Messenger; D043862:Rotaxanes; D013451:Sulfonic Acids; C475673:polyrotaxane; D020442:Poloxamer
keywords: Polyrotaxane; bone morphogenetic protein-2; osteogenic differentiation; surface tethering