title: Peroxidase-mediated formation of corn fiber gum-bovine serum albumin conjugates: Molecular and structural characterization.
abstract: Corn fiber gum (CFG) was previously conjugated with bovine serum albumin (BSA) via peroxidase treatment to improve its emulsifying properties. The current study was undertaken to evaluate the molecular characteristics of CFG-BSA conjugates prepared at different CFG/BSA weight ratios of 10:1, 4:1, 1:1, and 1:4. After the peroxidase treatment, CFG-BSA mixtures at weight ratios of 10:1 and 4:1 showed an increase in molecular weight from 200 and 193kDa to 218 and 223kDa respectively. But for CFG-BSA mixtures at weight ratio of 1:4, their molecular weight decreased. Peroxidase treated CFG-BSA mixtures had a more compact but rougher surface morphology as compared with the untreated as revealed by scanning electron microscopy. At lower CFG to BSA weight ratio (1:4), particles of CFG-BSA conjugates appeared to be elongated while at higher CFG to BSA weight ratio (10:1), they were approximately spherical in transmission electron micrographs.
authors.affiliation: Beijing Key Laboratory for Food Non-thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, PO Box 40, 17 Qinghuadonglu, Haidian, Beijing 100083, PR China. Electronic address: liuyan1990@cau.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, US Department of Agricultural, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. Electronic address: Madhav.Yadav@ARS.USDA.GOV.
authors.forename: Madhav P
authors.initials: MP
authors.lastname: Yadav
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, US Department of Agricultural, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. Electronic address: rose.chau@ars.usda.gov.
authors.forename: Hoa K
authors.initials: HK
authors.lastname: Chau
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, US Department of Agricultural, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. Electronic address: Shuang.Qiu@ARS.USDA.GOV.
authors.forename: Shuang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: Beijing Key Laboratory for Food Non-thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, PO Box 40, 17 Qinghuadonglu, Haidian, Beijing 100083, PR China. Electronic address: zhanghui@cau.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory for Food Non-thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, PO Box 40, 17 Qinghuadonglu, Haidian, Beijing 100083, PR China; College of Food Science and Technology, Henan University of Technology, 140 Songshananlu, Zhengzhou 450052, PR China. Electronic address: ljyin@cau.edu.cn.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yin
mesh_terms: D004655:Emulsions; D009195:Peroxidase / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D004655:Emulsions; D053149:Plant Gums; D012710:Serum Albumin, Bovine; D009195:Peroxidase
keywords: Bovine serum albumin; Corn fiber gum; Intermolecular conjugation; Molecular characterization; Structural characterization