title: Effects of inhibitors of transcription factors, nuclear factor-κB and activator protein 1, on the expression of proinflammatory cytokines and chemokines induced by stimulation with Toll-like receptor ligands in hen vaginal cells.
abstract: The goal of this study was to determine whether nuclear factor-κB (NFκB) and activator protein 1 (AP-1) were the responsible transcription factors for the induction of proinflammatory cytokines in hen vaginal cells stimulated by different Toll-like receptor (TLR) ligands. Cultured vaginal cells were treated with or without poly I:C (TLR3 ligand; dsRNA virus), lipopolysaccharide (LPS) (TLR4 ligand; gram-negative bacteria), flagellin (TLR5 ligand; bacterial flagellum), R848 (TLR7 ligand; ssRNA virus), and CpG-oligodeoxynucleotide (CpG-ODN) (TLR21 ligand; bacteria and DNA virus) in the presence or absence of different doses of BAY11-7085 (NFκB inhibitor) and tanshinone IIA (AP-1 inhibitor). Then, gene expressions of IL1B, IL6, and CXCLi2 were examined by real-time PCR analysis. The results showed that the induction of the expression of IL1B, IL6 and CXCLi2 by poly I:C, LPS, and CpG-ODN were suppressed by Bay11-7085, but not by tanshinone IIA. IL1B expression was upregulated by flagellin and R848, and the increase in its expression was suppressed by Bay11-7085, but not by tanshinone. These results suggest that NFκB is the responsible transcription factor for the expression of proinflammatory cytokines and chemokines, including I IL1B, IL6, and CXCLi2 in response to the ligands of TLR3, 4, and 21, and IL1B in response to the ligands of TLR5 and 7 in the vaginal cells.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kamimura
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.|The Research Center for Animal Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Isobe
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.|The Research Center for Animal Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008024:Ligands; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000276:immunology*; D010057:Oviducts / Q000382:microbiology; D051193:Toll-Like Receptors / Q000276:immunology*; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors / Q000276:immunology*; D014621:Vagina / Q000166:cytology
chemical_list: D030161:Avian Proteins; D018925:Chemokines; D016207:Cytokines; D007166:Immunosuppressive Agents; D008024:Ligands; D016328:NF-kappa B; D051193:Toll-Like Receptors; D018808:Transcription Factor AP-1
keywords: NFκB; Toll-like receptors; chicken oviduct; cytokines; transcription factors