title: Gastrodin protects against LPS-induced acute lung injury by activating Nrf2 signaling pathway.
abstract: Gastrodin (GAS), a phenolic glucoside derived from Gastrodiaelata Blume, has been reported to have anti-inflammatory effect. The aim of this study was to investigate the effects of GAS on LPS-induced acute lung injury in mice. ALI was induced by the intranasal administration of LPS and GAS was given 1 h or 12 h after LPS treatment. The results indicated that GAS treatment markedly attenuated the damage of lung injury induced by LPS. GAS attenuated the activity of myeloperoxidase (MPO) and down-regulated the levels of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β in BALF. LPS-induced lung edema and lung function were also reversed by GAS. Furthermore, GAS was found to inhibit LPS-induced inflammatory cells infiltration. In addition, treatment of GAS inhibited LPS-induced NF-κB activation and up-regulated the expression of Nrf2 and HO-1. In conclusion, our results indicated that GAS had anti-inflammatory effects on LPS-induced acute lung injury. The anti-inflammatory mechanism of GAS was through the inhibition of NF-κB and activation of Nrf2 signaling pathways.
authors.affiliation: Laboratory of Pharmacology, College of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Laboratory of Science of Chinese Pharmacology, College of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Laboratory of Pharmacology, College of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
authors.forename: Daqiang
authors.initials: D
authors.lastname: Song
authors.affiliation: Laboratory of Pharmacology, College of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Research Department, College of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
authors.forename: Changli
authors.initials: C
authors.lastname: Liao
authors.affiliation: Department of Anesthesiology, The Affiliated Hospital of College of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Jiang
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005960:Glucosides / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects*; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D012129:Respiratory Function Tests; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D001592:Benzyl Alcohols; D015415:Biomarkers; D016207:Cytokines; D005960:Glucosides; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; D020011:Protective Agents; C045345:gastrodin; D009195:Peroxidase; D051547:Heme Oxygenase-1
keywords: LPS; Nrf2; gastrodin; lung injury