title: The effect of chrysin-loaded nanofiber on wound healing process in male rat.
abstract: Wound healing is an inflammatory process. Chrysin, a natural flavonoid found in honey, has been recently investigated to have anti-inflammatory and antioxidant effects. In this work, the effects of chrysin-loaded nanofiber on the expressions of genes that are related to wound healing process such as P53, TIMPs, MMPs, iNOS, and IL-6 in an animal model study were evaluated. The electrospinning method was used for preparation the different concentrations of chrysin-loaded PCL-PEG nanofiber (5%, 10%, and 20% [w/w]) and characterized by FTIR and SEM. The wound healing effects of chrysin-loaded PCL-PEG nanofiber were in vivo investigated in rats, and the expressions of genes related to wound healing process were evaluated by real-time PCR. The study results showed chrysin-loaded PLC-PEG compared to chrysin ointment and control groups significantly increase IL-6, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 (p < .05). On the other hand, nanofibers containing chrysin significantly decreased p53 and iNOS expression compared to chrysin ointment and control groups (p < .05). According to the results, chrysin-loaded PCL-PEG-PCL nanofibers have positive effects on the expression of the genes that have pivotal role in wound healing.
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.|Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Zoheyr
authors.initials: Z
authors.lastname: Mohammadi
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sharif Zak
authors.affiliation: Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hasan
authors.initials: H
authors.lastname: Majdi
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Khaled
authors.initials: K
authors.lastname: Seidi
authors.affiliation: Student Research Committee, School of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Meisam
authors.initials: M
authors.lastname: Barati
authors.affiliation: Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abolfazl
authors.identifier: http://orcid.org/0000-0001-9941-0357
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Latifi
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020784:Matrix Metalloproteinase 8 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004337:Drug Carriers; D005419:Flavonoids; D011091:Polyesters; D019715:Tissue Inhibitor of Metalloproteinase-1; D016159:Tumor Suppressor Protein p53; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D019716:Tissue Inhibitor of Metalloproteinase-2; C043561:chrysin; D011092:Polyethylene Glycols; D052247:Nitric Oxide Synthase Type II; D020778:Matrix Metalloproteinase 2; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9
keywords: chrysin; chrysin-loaded PCL-PEG-PCL nanofiber; gene expression; open wound healing