title: In vivoMitophagy Monitoring inCaenorhabditis elegansto Determine Mitochondrial Homeostasis.
abstract: Perturbation of mitochondrial function is a major hallmark of several pathological conditions and ageing, underlining the essential role of fine-tuned mitochondrial activity (Lopez-Otinet al., 2013). Mitochondrial selective autophagy, known as mitophagy, mediates the removal of dysfunctional and/or superfluous organelles, preserving cellular and organismal homeostasis (Palikaras and Tavernarakis, 2014; Pickrell and Youle, 2015; Scheibye-Knudsenet al., 2015). In this protocol, we describe a method for assessing mitophagy in the nematodeCaenorhabditis elegans.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Palikaras
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Tavernarakis
keywords: Ageing; Autophagosome; Autophagy; Caenorhabditis elegans; Lysosomes; Mitochondria; Mitophagy; mtRosella