title: Efficacy and speed of kill of a new spot-on formulation of selamectin plus sarolaner against flea infestations in cats.
abstract: The efficacy of a new spot-on formulation of selamectin plus sarolaner against induced flea infestations in cats was confirmed in three placebo-controlled, blinded studies. Purpose-bred adult cats (n=8/group) were blocked by pre-treatment flea counts and randomly allocated to treatment with either a placebo or with the spot-on formulation at the minimum dose of 6.0mg selamectin and 1.0mg sarolaner per kg bodyweight. Treatments were applied topically once on Day 0. All cats were infested with approximately 100 unfed, adult Ctenocephalides felis prior to treatment and at weekly intervals for 5 weeks. In Studies 1 and 2 comb counts were conducted to determine the numbers of viable fleas 24h after treatment and subsequent weekly infestations. In Study 3, flea counts were conducted at 6, 12, 24 and 48h after treatment and 3, 6, 12 and 24h after subsequent weekly infestations to evaluate the speed of kill against fleas. Cats in the placebo-treated groups maintained flea infestations throughout all studies. In Study 1, no live fleas were found on any of the treated cats, resulting in 100% efficacy for 5 weeks after a single treatment (P≤0.0001). In Study 2, selamectin/sarolaner reduced flea counts by 92.4% immediately after treatment and by 97.7%-100% after re-infestations for five weeks (P≤0.0001). In the speed of kill study, selamectin/sarolaner started killing fleas within 12h after treatment administration and within 6h following re-infestation for at least 28days. Efficacy was 98.1% by 24h after treatment and 100% within 24h after re-infestations for 5 weeks. A single topical administration of a new spot-on formulation of selamectin plus sarolaner at the minimum dose rapidly and consistently kills fleas on cats for at least 5 weeks.
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, Mercuriusstraat 20, Zaventem B-1930, Belgium. Electronic address: csilla.becskei@zoetis.com.
authors.forename: Csilla
authors.initials: C
authors.lastname: Becskei
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Cherni
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Adriano F
authors.initials: AF
authors.lastname: Vatta
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Vickie L
authors.initials: VL
authors.lastname: King
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, Mercuriusstraat 20, Zaventem B-1930, Belgium.
authors.forename: Dan
authors.initials: D
authors.lastname: Lin
authors.affiliation: Zoetis, Veterinary Medicine Research and Development, 333 Portage Street, Kalamazoo, MI 49007, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Rugg
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000977:Antiparasitic Agents / Q000008:administration & dosage; D002371:Cat Diseases / Q000188:drug therapy*; D002415:Cats; D005260:Female; D058267:Flea Infestations / Q000188:drug therapy / Q000662:veterinary*; D007555:Isoxazoles / Q000008:administration & dosage*; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008297:Male; D011897:Random Allocation; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000977:Antiparasitic Agents; D007555:Isoxazoles; D007559:Ivermectin; C414354:selamectin
keywords: Cat; Flea; Revolution; Sarolaner; Selamectin; Speed of kill; Stronghold