title: PCB 118-induced endothelial cell apoptosis is partially mediated by excessive ROS production.
abstract: Endothelial cell apoptosis, which may alter the integrity of the endothelium and lead to plaque instability, plays a critical role in the development and pathogenesis of atherosclerosis. Exposure of polychlorinated biphenyls (PCBs) is associated with increased risk of atherosclerosis and cardiovascular disease. In our present study, we explored whether exposure to PCB 118 influences endothelial cell apoptosis in vitro and the underlying mechanisms involved. As expected, exposure to PCB 118 increased the intracellular reactive oxygen species (ROS) levels in HUVECs. Increases in apoptosis and Bax/Bcl-2 ratios were observed in PCB 118-treated HUVECs. N-acetyl-l-cysteine (NAC), a ROS scavenger, partially reduced PCB 118-induced apoptosis and Bax/Bcl-2 ratios in HUVECs. Taken together, PCB 118-induced endothelial cell apoptosis was partially initiated by excessive ROS production.
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.affiliation: b Department of Dermatology , The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Jin-Nan
authors.initials: JN
authors.lastname: Cheng
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.|c Department of Endocrinology , The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Long
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: He
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Guan-Nan
authors.initials: GN
authors.lastname: Liang
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Tang
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.|c Department of Endocrinology , The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Jiang
authors.affiliation: a Experimental Medicine Center, The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan , PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; C070055:2,3',4,4',5-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls
keywords: 2,3′,4,4′,5-pentachlorobiphenyl; Polychlorinated biphenyls; ROS; apoptosis; endothelial cell