title: Inhibition ofPseudomonas aeruginosaBiofilm Formation by Traditional Chinese Medicinal HerbHerba patriniae.
abstract: New antimicrobial agents are urgently needed to treat infections caused by drug-resistant pathogens and by pathogens capable of persisting in biofilms. The aim of this study was to identify traditional Chinese herbs that could inhibit biofilm formation ofPseudomonas aeruginosa, an important human pathogen that causes serious and difficult-to-treat infections in humans. AluxCDABE-based reporter system was constructed to monitor the expression of six key biofilm-associated genes inP. aeruginosa. The reporters were used to screen a library of 36 herb extracts for inhibitory properties against these genes. The results obtained indicated that the extract ofHerba patriniaedisplayed significant inhibitory effect on almost all of these biofilm-associated genes. Quantitative analysis showed thatH. patriniaeextract was able to significantly reduce the biofilm formation and dramatically altered the structure of the mature biofilms ofP. aeruginosa. Further studies showedH. patriniaeextract decreased exopolysaccharide production byP. aeruginosaand promoted its swarming motility, two features disparately associated with biofilm formation. These results provided a potential mechanism for the use ofH. patriniaeto treat bacterial infections by traditional Chinese medicines and revealed a promising candidate for exploration of new drugs againstP. aeruginosabiofilm-associated infections.
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Qiaolian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Minyan
authors.initials: M
authors.lastname: Dang
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Dangdang
authors.initials: D
authors.lastname: Bai
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Lixin
authors.identifier: 0000-0002-7273-2847
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China; Department of Oral Biology & Medical Microbiology, Rady Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Ave., Winnipeg, MB, Canada R3E 0W2.
authors.forename: Kangmin
authors.identifier: 0000-0002-3016-786X
authors.initials: K
authors.lastname: Duan
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects*; D006801:Humans; D008516:Medicine, Chinese Traditional / Q000379:methods; D032405:Patrinia / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts