title: Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis.
abstract: Lindane is an organochlorine pesticide belonging to persistent organic pollutants (POPs) that has been widely used to treat agricultural pests. It is of particular concern because of its toxicity, persistence and tendency to bioaccumulate in terrestrial and aquatic ecosystems. In this context, we assessed the role of bacteria associated with the spongeHymeniacidon perlevisin lindane degradation. Seven bacteria isolates were characterized and identified. These isolates showed a remarkable capacity to utilize lindane as a sole carbon source leading to a percentage of residual lindane ranging from 3% to 13% after 12 days of incubation with the pesticide. The lindane metabolite, 1,3-6-pentachloro-cyclohexene, was identified as result of lindane degradation and determined by gas chromatography-mass spectrometry (GC-MS). The bacteria capable of lindane degradation were identified on the basis of the phenotypic characterization by morphological, biochemical and cultural tests, completed with 16S rDNA sequence analysis, and assigned toMameliella phaeodactyli,Pseudovibrioascidiaceicola,Oceanicaulis stylophorae,Ruegeria atlanticaand to three new uncharacterized species. The results obtained are a prelude to the development of future strategies for the in situ bioremediation of lindane.
authors.affiliation: Istituto per l'Ambiente Marino Costiero, Unità Operativa di Supporto di Taranto, CNR, Via Roma 3, 74123 Taranto, Italy. loredana.stabili@iamc.cnr.it.|Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, Italy. loredana.stabili@iamc.cnr.it.
authors.forename: Stabili
authors.initials: S
authors.lastname: Loredana
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, Italy. graziano.pizolante@unisalento.it.
authors.forename: Pizzolante
authors.initials: P
authors.lastname: Graziano
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, Italy. anto.mrg88@gmail.com.
authors.forename: Morgante
authors.initials: M
authors.lastname: Antonio
authors.affiliation: Dipartimento di Biologia, Università di Bari Aldo Moro, 70125 Bari, Italy. carlotta.nonnismarzano@uniba.it.|CoNISMa, Piazzale Flaminio 9, 00196 Roma, Italy. carlotta.nonnismarzano@uniba.it.
authors.forename: Nonnis Marzano
authors.initials: NM
authors.lastname: Carlotta
authors.affiliation: Dipartimento di Biologia, Università di Bari Aldo Moro, 70125 Bari, Italy. caterina.longo@uniba.it.|CoNISMa, Piazzale Flaminio 9, 00196 Roma, Italy. caterina.longo@uniba.it.
authors.forename: Longo
authors.initials: L
authors.lastname: Caterina
authors.affiliation: Dipartimento di Chimica, Università di Bari Aldo Moro, 70125 Bari, Italy. antonellamaria.aresta@uniba.it.
authors.forename: Aresta Antonella
authors.initials: AA
authors.lastname: Maria
authors.affiliation: Dipartimento di Chimica, Università di Bari Aldo Moro, 70125 Bari, Italy. carlo.zambonin@uniba.it.
authors.forename: Zambonin
authors.initials: Z
authors.lastname: Carlo
authors.affiliation: Dipartimento di Biologia, Università di Bari Aldo Moro, 70125 Bari, Italy. giuseppe.corriero@uniba.it.|CoNISMa, Piazzale Flaminio 9, 00196 Roma, Italy. giuseppe.corriero@uniba.it.
authors.forename: Corriero
authors.initials: C
authors.lastname: Giuseppe
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, Italy. pietro.alifano@unisalento.it.
authors.forename: Alifano
authors.initials: A
authors.lastname: Pietro
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism; D010575:Pesticides / Q000378:metabolism; D011161:Porifera / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology
chemical_list: D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D001556:Hexachlorocyclohexane
keywords: 1, 3–6-pentachloro-cyclohexene; H. perlevis; bioremediation; gas chromatography–mass spectrometry (GC–MS); lindane; sponge-associated bacteria