title: Mesenchymal stem cells cannot affect mRNA expression of toll-like receptors in different tissues during sepsis.
abstract: OBJECTIVE
  Experimental animal models and human clinical studies support a crucial role for TLRs in infectious diseases. The aim of this study was to test the ability of MSCs, which have immunomodulatory effects, of altering the mRNA expression of toll-like receptors during a experimental model of sepsis in different tissues.
  
  
  METHODS
  Three experimental groups (male C57BL/6 mice) were formed for the test: control group, untreated septic group and septic group treated with MSCs (1 × 106cells/animal). Lungs, cortex, kidney, liver and colon tissue were dissected after 12 h of sepsis induction and TLR2/3/4/9 mRNA were evaluated by RT-qPCR.
  
  
  RESULTS
  We observed a decrease of TLR2 and 9 mRNA expression in the liver of the sepsis group, while TLR3 was decreased in the lung and liver. No change was found between the sepsis group and the sepsis + MSC group.
  
  
  CONCLUSIONS
  In this model of experimental sepsis the MSCs were unable to modify the mRNA expression of the different toll-like receptors evaluated.
authors.affiliation: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil. leopedrazza@gmail.com.
authors.forename: Leonardo
authors.identifier: http://orcid.org/0000-0001-6815-9366
authors.initials: L
authors.lastname: Pedrazza
authors.affiliation: Laboratório de Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
authors.forename: Talita Carneiro Brandão
authors.initials: TCB
authors.lastname: Pereira
authors.affiliation: Programa de Graduação de Biotecnologia, Centro Universitário Univates, Lajeado, Rio Grande do Sul, CEP 95900-000, Brazil.
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Abujamra
authors.affiliation: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
authors.forename: Fernanda Bordignon
authors.initials: FB
authors.lastname: Nunes
authors.affiliation: Laboratório de Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
authors.affiliation: Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
authors.forename: Jarbas Rodrigues
authors.initials: JR
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D018805:Sepsis / Q000235:genetics* / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D051193:Toll-Like Receptors
keywords: Mesenchymal stem cells; Sepsis; Toll-like receptors