title: Clinical significance of the correlation between PLCE 1 and PRKCA in esophageal inflammation and esophageal carcinoma.
abstract: Esophagitis and Barrett's esophagus are linked to esophageal squamous cell carcinoma and adenocarcinoma, respectively. However, the underlying mechanisms are still unclear. This study analyzed the expression levels of and correlation between PLCE1 and PRKCA in human esophagitis, carcinogen NMBA-induced rat esophagus, PLCE1 genetic deficient mouse esophageal epithelial tissues and human esophageal cancer cell line, integrated with Online oncology data sets. We found that the expression levels of both PLCE1 and PRKCA were significantly elevated in human esophagitis, esophageal squamous cell carcinoma, Barrett's esophagus, esophageal adenocarcinoma and in NMBA-treated rat esophageal epithelia. However, PRKCA and cytokines were significantly downregulated in PLCE1-deficient mouse esophageal epithelia, and knockdown of PLCE1 in human esophageal cancer cells led to reduction of PRKCA and cytokines. Finally, high expression of both PLCE1 and PRKCA is significantly associated with poor outcomes of the patients with esophageal cancers. In conclusion, this study defined the initiation and progression of esophageal inflammation and malignant transformation, in which the positive correlation of PLCE1 and PRKCA exhibits critical clinical significance.
authors.affiliation: Department of Pathology and Institute of Precision Medicine, Jining Medical University, Jining 272067, China.
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Pathology and Institute of Precision Medicine, Jining Medical University, Jining 272067, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital, Jining Medical University, Jining 272067, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Pathology, Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Yongmeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Department of Pathology and Institute of Precision Medicine, Jining Medical University, Jining 272067, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Pathology and Institute of Precision Medicine, Jining Medical University, Jining 272067, China.
authors.forename: Yiqiong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pathology and Institute of Precision Medicine, Jining Medical University, Jining 272067, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Ansong
authors.affiliation: Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Huali
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Virgilia
authors.initials: V
authors.lastname: Macias
authors.affiliation: Department of Pathology and Institute of Precision Medicine, Jining Medical University, Jining 272067, China.|Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Wancai
authors.initials: W
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001471:Barrett Esophagus / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D001706:Biopsy; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D016207:Cytokines / Q000378:metabolism; D030541:Databases, Genetic; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D004941:Esophagitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004947:Esophagus / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D054801:Phosphoinositide Phospholipase C / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D051571:Protein Kinase C-alpha / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon
keywords: Barrett's esophagus; PLCE1; adenocarcinoma; esophagitis; squamous cell carcinoma