title: New ursane-type triterpenoids from Clerodendranthus spicatus.
abstract: Five new ursane-type triterpenoids, spicatusoids A-E (1, 3-6), and three known ones (2, 7, and 8), and a known oleanane-type triterpenoid (9) were isolated from the aerial parts of Clerodendranthus spicatus. Their structures were elucidated by spectroscopic methods. In particular, the structure of 3 including its absolute configuration was confirmed by single-crystal X-ray diffraction analysis. Cell viability of all the compounds against rat kidney fibroblast cells (NRK-49F) with or without TGF-β1 induction and human cancer cells (HL-60, SMMC-7721, A-549, MCF-7, and SW-480) was examined by using MTT or MST assays. It was found that, with exception of 1, all the tested compounds could inhibit cell proliferation in TGF-β1 induced NRK-49F cells with compounds 2 being most active.
authors.affiliation: Southwest Medical University, Luzhou 646000, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; Guangdong Pharmaceutical University, Guangzhou 5100069, PR China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; University of Chinese Academy of Sciences, Yuquan Road 19, Beijing 100049, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Yan
authors.affiliation: Guangdong Pharmaceutical University, Guangzhou 5100069, PR China.
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Southwest Medical University, Luzhou 646000, PR China. Electronic address: 496655521@qq.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China. Electronic address: yxcheng@mail.kib.ac.cn.
authors.forename: Yong-Xian
authors.initials: YX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification; D053978:Pentacyclic Triterpenes
chemical_list: D009828:Oleanolic Acid; D053773:Transforming Growth Factor beta1; D014315:Triterpenes; C413246:oleanane; C000606873:ursane; D053978:Pentacyclic Triterpenes
keywords: Clerodendranthus spicatus; Human cancer cells; Kidney fibroblast cells; Ursane-type triterpenoids