title: The t(8;14)(q24.1;q32) and its variant translocations: A study of 34 cases.
abstract: BACKGROUND
  The t(8;14)(q24.1;q32) and its variants - the t(2;8)(p12;q24.1) and t(8;22)(q24.1;q11.2) are associated with B-cell neoplasia and result in MYC/immunoglobulin (IG) gene rearrangement.
  
  
  METHODS
  We correlated the cytogenetic, molecular and clinico-pathological findings of patients with 8q24 translocations seen in the Department of Haematology, Christian Medical College, Vellore, from January 2003 to December 2015.
  
  
  RESULTS
  There were 34 patients with 8q24 translocations (31, ALL and three myeloma). The t(8;14) was seen in 25 patients, t(8;22) in seven and t(2;8) in two. The salient findings were as follows: 85% males; 79% adults, median age 37 years; L3 morphology in 61%; mature B immunophenotype in 77%; extra-medullary disease in 41%; additional abnormalities in 28 (85%), notably, structural abnormalities of chromosome 1q (41%) and 13q (9%) and monosomy 13 (15%); complex karyotypes in 68%. There were two double-hit lymphoma/leukemia, one with a t(14;18)(q32;q21) and the other with a t(3;14)(q27;q11.2), associated with nodal high grade B cell lymphoma and dermal leukemic infiltrates respectively. Only 13 samples were processed for DNA PCR and all these samples were positive for MYC-IgH (c-gamma type) rearrangement. Only in one patient, in addition to c-gamma, c-alpha rearrangement was also detected.
  
  
  CONCLUSIONS
  The frequency (1.7%) and distribution of these translocations in our series and the association with 1q and 13q abnormalities is similar to the literature. Trisomies 7 and 12 were seen in less than 10% of our patients.
authors.affiliation: Cytogenetics Unit, Christian Medical College & Hospital, Vellore, Tamil Nadu 632004, India.
authors.forename: Meenu
authors.initials: M
authors.lastname: Angi
authors.affiliation: Cytogenetics Unit, Christian Medical College & Hospital, Vellore, Tamil Nadu 632004, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Kamath
authors.affiliation: Cytogenetics Unit, Christian Medical College & Hospital, Vellore, Tamil Nadu 632004, India.
authors.forename: S
authors.initials: S
authors.lastname: Yuvarani
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: J
authors.initials: J
authors.lastname: Meena
authors.affiliation: Department of Transfusion Medicine and Immunohematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Usha
authors.initials: U
authors.lastname: Sitaram
authors.affiliation: Department of Pathology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Marie Therese
authors.initials: MT
authors.lastname: Manipadam
authors.affiliation: Department of Transfusion Medicine and Immunohematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Sukesh
authors.initials: S
authors.lastname: Nair
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Abhijeet
authors.initials: A
authors.lastname: Ganapule
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: N A
authors.initials: NA
authors.lastname: Fouzia
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Aby
authors.initials: A
authors.lastname: Abraham
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Auro
authors.initials: A
authors.lastname: Viswabandya
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: B
authors.initials: B
authors.lastname: Poonkuzhali
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Biju
authors.initials: B
authors.lastname: George
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Mathews
authors.affiliation: Department of Hematology, Christian Medical College & Hospital, Vellore, Tamil Nadu, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Cytogenetics Unit, Christian Medical College & Hospital, Vellore, Tamil Nadu 632004, India. Electronic address: cytogen@cmcvellore.ac.in.
authors.forename: Vivi M
authors.initials: VM
authors.lastname: Srivastava
mesh_terms: D000328:Adult; D002051:Burkitt Lymphoma / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D014178:Translocation, Genetic / Q000235:genetics*
keywords: 8q24 translocations; Burkitt leukemia/lymphoma; Complex karyotype; Double-hit leukemia/lymphoma; MYC/immunoglobulin gene rearrangement