title: Evaluation of a Panel of Single-Nucleotide Polymorphisms in miR-146a and miR-196a2 Genomic Regions in Patients with Chronic Periodontitis.
abstract: BACKGROUND
  Periodontitis is an inflammatory disease caused by bacterial triggering of the host immune-inflammatory response, which in turn is regulated by microRNAs (miRNA). Polymorphisms in the miRNA pathways affect the expression of several target genes such as tumor necrosis factor-α and interleukins, which are associated with progression of disease.
  
  
  OBJECTIVE
  The objective of this study was to identify the association between the MiR-146a single nucleotide polymorphisms (SNPs) (rs2910164, rs57095329, and rs73318382), the MiR-196a2 (rs11614913) SNP and chronic periodontitis.
  
  
  METHODS
  Genotyping was performed for the MiR-146a (rs2910164, rs57095329, and rs73318382) and the MiR-196a2 (rs11614913) polymorphisms in 180 healthy controls and 190 cases of chronic periodontitis by the direct Sanger sequencing technique. The strength of the association between the polymorphisms and chronic periodontitis was evaluated using logistic regression analysis. Haplotype and linkage analyses among the polymorphisms was performed. Multifactorial dimensionality reduction was performed to determine epistatic interaction among the polymorphisms.
  
  
  RESULTS
  The MiR-196a2 polymorphism revealed a significant inverse association with chronic periodontitis. Haplotype analysis of MiR-146a and MiR-196a2 polymorphisms revealed 13 different combinations, of which 5 were found to have an inverse association with chronic periodontitis.
  
  
  CONCLUSIONS
  The present study has demonstrated a significant inverse association of MiR-196a2 polymorphism with chronic periodontitis.
authors.affiliation: 1 Department of Human Genetics, Sri Ramachandra University , Chennai, India .
authors.forename: Priyanka
authors.initials: P
authors.lastname: Venugopal
authors.affiliation: 2 Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University , Chennai, India .
authors.forename: Vamsi
authors.initials: V
authors.lastname: Lavu
authors.affiliation: 2 Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University , Chennai, India .
authors.forename: Suresh
authors.initials: S
authors.lastname: RangaRao
authors.affiliation: 1 Department of Human Genetics, Sri Ramachandra University , Chennai, India .
authors.forename: Vettriselvi
authors.initials: V
authors.lastname: Venkatesan
mesh_terms: D000328:Adult; D055113:Chronic Periodontitis / Q000209:etiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007194:India; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009711:Nucleotides; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; D035683:MicroRNAs; D009711:Nucleotides; D014409:Tumor Necrosis Factor-alpha
keywords: chronic periodontitis; genotype; microRNA; single-nucleotide polymorphisms