title: Enhancing colour and oxidative stabilities of reduced-nitrite turkey meat sausages during refrigerated storage using fucoxanthin purified from the Tunisian seaweed Cystoseira barbata.
abstract: The present study investigated the angiotensin-I converting enzyme (ACE) inhibitory activity and the antioxidant properties, in vitro and in cured meat sausages containing reduced levels of sodium nitrite, of fucoxanthin extracted from the Tunisian brown seaweed Cystoseira barbata (CBFX). Results revealed that CBFX exhibited great scavenging activities against DPPH free radicals (EC50= 136 μg/ml), peroxyl radicals in the linoleate-β-carotene system (EC50= 43 μg/ml) and hydroxyl radicals generated by Fenton reaction (DNA nicking assay). A considerable ferric reducing potential was also recorded for CBFX (EC50= 34 μg/ml). It is interesting to note that CBFX was found to modulate the ACE activity, which is the key enzyme involved in the blood pressure regulation, with an EC50of 5 μg/ml. When fucoxanthin was supplemented, the concentration of sodium nitrite added to cured turkey meat sausages was reduced from 150 to 80 ppm, coupled with the enhancement of colour and oxidative stabilities. Thus, CBFX, with noticeable antioxidant and antihyertensive effects, could be used as a natural additive in functional foods to alleviate potential human health hazards caused by carcinogenic nitrosamines formation.
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia. Electronic address: sabrine.sellimi@gmail.com.
authors.forename: Sabrine
authors.initials: S
authors.lastname: Sellimi
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Ghada
authors.initials: G
authors.lastname: Ksouda
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Abdelkarim
authors.initials: A
authors.lastname: Benslima
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Nasri
authors.affiliation: Biomaterials Applications, 6, rue Lesdiguières 38000 Grenoble, France.
authors.forename: Marguerite
authors.initials: M
authors.lastname: Rinaudo
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hajji
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000032:analysis / Q000302:isolation & purification; D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification; D003116:Color; D005503:Food Additives / Q000737:chemistry*; D061353:Food Storage; D008461:Meat Products / Q000032:analysis*; D010084:Oxidation-Reduction; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D012624:Seaweed / Q000737:chemistry; D012977:Sodium Nitrite / Q000032:analysis* / Q000302:isolation & purification; D014422:Turkeys; D024341:Xanthophylls / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000959:Antihypertensive Agents; D000975:Antioxidants; D005503:Food Additives; D010936:Plant Extracts; D024341:Xanthophylls; C025164:fucoxanthin; D012977:Sodium Nitrite
keywords: ACE inhibitory activity; Antioxidant activity; Cystoseira barbata; Fucoxanthin; Lipid peroxidation; Turkey meat sausage