title: Proteomic analysis of Arabidopsis plasma membranes reveals lipopolysaccharide-responsive changes.
abstract: Plant plasma membranes (PMs) contain pattern recognition receptors (PRRs), lately believed to be associated within multicomponent complexes, which perceive microbe-associated molecular pattern (MAMP) molecules like lipopolysaccharides (LPSs) and result in signal transduction events that lead to activated immune defense responses. As such, Arabidopsis thaliana leaves were treated with LPS from Escherichia coli (LPSE.coli) over time, and PM fractions isolated and evaluated using gel-based and subsequent mass spectrometry approaches for identification of LPS-responsive proteins. From the identified protein bands and spots, it is concluded that perception of hexaacylated LPS and resulting signal transduction occurs via PM-associated protein(s), amongst others, receptor-like kinases (RLKs) including G-type lectin S-receptor kinase and BAK1, and mostly likely within specialized perception domains.
authors.affiliation: Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa.
authors.forename: Nwaxigombe M
authors.initials: NM
authors.lastname: Baloyi
authors.affiliation: Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa.
authors.forename: Ian A
authors.initials: IA
authors.lastname: Dubery
authors.affiliation: Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa. Electronic address: lpiater@uj.ac.za.
authors.forename: Lizelle A
authors.initials: LA
authors.lastname: Piater
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000493:pharmacokinetics*; D008565:Membrane Proteins / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D020543:Proteome
keywords: Lipopolysaccharide; Plasma membrane; Proteomics