title: Evaluation of efficient glucose release using sodium hydroxide and phosphoric acid as pretreating agents from the biomass of Sesbania grandiflora (L.) Pers.: A fast growing tree legume.
abstract: Sesbania grandiflora (L.) Pers. is one of the fast growing tree legumes having the efficiency to produce around 50tha-1above ground dry matters in a year. In this study, biomass of 2years old S. grandiflora was selected for the chemical composition, pretreatments and enzymatic hydrolysis studies. The stem biomass with a wood density of 3.89±0.01gmcm-3contains about 38% cellulose, 12% hemicellulose and 28% lignin. Enzymatic hydrolysis of pretreated biomass revealed that phosphoric acid (H3PO4) pretreated samples even at lower cellulase loadings [1 Filter Paper Units (FPU)], could efficiently convert about 86% glucose, while, even at higher cellulase loadings (60FPU) alkali pretreated biomass could convert only about 58% glucose. The effectiveness of phosphoric acid pretreatment was also supported by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) analysis.
authors.affiliation: Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India.
authors.forename: Nitesh K
authors.initials: NK
authors.lastname: Mund
authors.affiliation: Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Dash
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Chitta R
authors.initials: CR
authors.lastname: Barik
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Vaibhav V
authors.initials: VV
authors.lastname: Goud
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Lingaraj
authors.initials: L
authors.lastname: Sahoo
authors.affiliation: AICRP on Agroforestry, College of Forestry, Orissa University of Agriculture and Technology, Bhubaneswar 751016, Odisha, India.
authors.forename: Prasannajit
authors.initials: P
authors.lastname: Mishra
authors.affiliation: Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India. Electronic address: niharnayak@yahoo.com.
authors.forename: Nihar R
authors.initials: NR
authors.lastname: Nayak
mesh_terms: D018533:Biomass; D002480:Cellulase; D002482:Cellulose / Q000737:chemistry; D007887:Fabaceae; D005947:Glucose; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D010756:Phosphoric Acids; D049931:Sesbania; D012972:Sodium Hydroxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010756:Phosphoric Acids; D012972:Sodium Hydroxide; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; D005947:Glucose
keywords: Biomass composition; Enzymatic saccharification; Pretreatments; Sesbania grandiflora