title: Enhancement in multiple lignolytic enzymes production for optimized lignin degradation and selectivity in fungal pretreatment of sweet sorghum bagasse.
abstract: The objective of this work was to study the increase in multiple lignolytic enzyme productions through the use of supplements in combination in pretreatment of sweet sorghum bagasse (SSB) by Coriolus versicolor such that enzymes act synergistically to maximize the lignin degradation and selectivity. Enzyme activities were enhanced by metallic salts and phenolic compound supplements in SSF. Supplement of syringic acid increased the activities of LiP, AAO and laccase; gallic acid increased MnP; CuSO4increased laccase and PPO to improve the lignin degradations and selectivity individually, higher than control. Combination of supplements optimized by RSM increased the production of laccase, LiP, MnP, PPO and AAO by 17.2, 45.5, 3.5, 2.4 and 3.6 folds respectively for synergistic action leading to highest lignin degradation (2.3 folds) and selectivity (7.1 folds). Enzymatic hydrolysis of pretreated SSB yielded ∼2.43 times fermentable sugar. This technique could be widely applied for pretreatment and enzyme productions.
authors.affiliation: Department of Biotechnology, Dr B R A National Institute of Technology, Jalandhar 144011, Punjab, India.
authors.forename: Vartika
authors.initials: V
authors.lastname: Mishra
authors.affiliation: Department of Biotechnology, Dr B R A National Institute of Technology, Jalandhar 144011, Punjab, India. Electronic address: janaak@nitj.ac.in.
authors.forename: Asim K
authors.initials: AK
authors.lastname: Jana
authors.affiliation: Department of Chemistry, Dr B R A National Institute of Technology, Jalandhar 144011, Punjab, India.
authors.forename: Mithu Maiti
authors.initials: MM
authors.lastname: Jana
authors.affiliation: Department of Biotechnology, Dr B R A National Institute of Technology, Jalandhar 144011, Punjab, India.
authors.forename: Antriksh
authors.initials: A
authors.lastname: Gupta
mesh_terms: D005658:Fungi / Q000378:metabolism; D006868:Hydrolysis; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D045868:Sorghum / Q000378:metabolism*
chemical_list: D008031:Lignin; D042845:Laccase
keywords: Fungal pretreatment; Lignin degradation; Lignocellulolytic enzymes; Lignocellulosic biomass; Optimization; Supplements