title: Controlled Dual Delivery of Angiogenin and Curcumin by Electrospun Nanofibers for Skin Regeneration.
abstract: In this study, we successfully fabricated a novel drug and plasmid DNA (pDNA) dual delivery system by electrospinning the dispersion composed of polyethyleneimine-carboxymethyl chitosan/pDNA-angiogenin (ANG) nanoparticles, curcumin (Cur), poly (D, L-lactic-co-glycolic acid) (PLGA), and cellulose nanocrystals (CNCs). In vitro release studies showed that the bioactivity of Cur and ANG was preserved in the nanofibers, and a sequential release pattern was achieved in which nearly 90% of the Cur was released in ca. 6 days and the ANG release lasted up to about 20 days. In vitro cell culture results suggested that the composite nanofibers exhibit excellent biocompatibility. To evaluate the in vivo angiogenesis and anti-infection properties, the PLGA/CNC/Cur/pDNA-ANG composite nanofibers were transplanted into the infected full-thickness burn wounds. Biopsy specimens were harvested for histology, immunohistochemistry, immunofluorescence, real-time quantitative PCR, and Western blotting analyses. The results indicated that the PLGA/CNC/Cur/pDNA-ANG composite nanofibers not only prevented local infection but also promoted skin regeneration.
authors.affiliation: 1 Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University , Guangzhou, China .|2 Department of Biomedical Engineering, Jinan University , Guangzhou, China .
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Mo
authors.affiliation: 1 Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University , Guangzhou, China .|2 Department of Biomedical Engineering, Jinan University , Guangzhou, China .
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: 1 Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University , Guangzhou, China .|2 Department of Biomedical Engineering, Jinan University , Guangzhou, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University , Guangzhou, China .|2 Department of Biomedical Engineering, Jinan University , Guangzhou, China .
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.affiliation: 3 Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Command , Guangzhou, China .
authors.forename: Biao
authors.initials: B
authors.lastname: Cheng
authors.affiliation: 4 Department of Chemistry, Jinan University , Guangzhou, China .
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D012879:Skin Physiological Phenomena / Q000187:drug effects*
chemical_list: D003692:Delayed-Action Preparations; C046366:angiogenin; D012259:Ribonuclease, Pancreatic; D003474:Curcumin
keywords: angiogenesis; gene therapy; infection; nanofibers; skin regeneration