title: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic.
abstract: Neuro-osteological interactions have an important role in the regulation of bone metabolism and regeneration. Neuropeptides combined with porous biphasic calcium phosphates (BCP) using protein adsorption may contribute to the acceleration of bone formation. In the present study, we investigated the effect of BCP combined with nerve growth factor (NGF) on the growth of osteoblasts in vitro and the combinational therapeutic effect on the repair of calvarial defects in vivo. NGF was separated and purified from Chinese cobra venom using a simplified three-step chromatography method. BCP combined with NGF exerted a potent effect on osteoblast differentiation, as evidenced by enhanced cell proliferation, increased ALP activity and the up-regulated expression of osteogenesis-related genes and proteins. Further, combinational therapy with BCP and NGF improved calvarial regeneration, which was superior to treatment with therapy alone, as observed using imageological and morphological examination and histological and immunohistochemical staining. The results confirmed the effect of neuro-osteological interactions through combinatorial treatment with NGF and BCP to promote osteogenesis and bone formation, which may provide an effective and economical strategy for clinical application.
authors.affiliation: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.|Guangxi Collaborative Innovation Center for Biomedicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.|Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Jin
authors.affiliation: The Medical and Scientific Research Center, Guangxi Medical University, Nanning, People's Republic of China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Yin
authors.affiliation: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.|Guangxi Collaborative Innovation Center for Biomedicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.|Guangxi Collaborative Innovation Center for Biomedicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.|Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry; D002478:Cells, Cultured; D004546:Elapid Venoms / Q000737:chemistry*; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000627:therapeutic use; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D001672:Biocompatible Materials; C558777:BoneCeramic; D002130:Calcium Phosphates; D004546:Elapid Venoms; D006882:Hydroxyapatites; D020932:Nerve Growth Factor