title: Periodontal Ligament Stem Cells Regulate Apoptosis of Neutrophils.
abstract: Periodontal ligament stem cells (PDLSCs) are promising cell resource for the cell-based therapy for periodontitis and regeneration of bio-root. In this study, we investigated the effect of PDLSCs on neutrophil, a critical constituent of innate immunity, and the underlying mechanisms. The effect of PDLSCs on the proliferation and apoptosis of resting neutrophils and IL-8 activated neutrophils was tested under cell-cell contact culture and Transwell culture, with or without anti-IL-6 neutralizing antibody. We found that PDLSCs could promote the proliferation and reduce the apoptosis of neutrophils whether under cell-cell contact or Transwell culture. Anti-IL-6 antibody reduced PDLSCs-mediated inhibition of neutrophil apoptosis. IL-6 at the concentration of 10ng/ml and 20ng/ml could inhibit neutrophil apoptosis statistically. Collectively, PDLSCs could reduce the apoptosis of neutrophils via IL-6.
authors.affiliation: Department of Implantology, Shandong Provincial Key Laboratory of Oral Biomedicine, Shandong University School of Stomatology, Jinan250012, Republic of China; Department of Dentistry, Weifang People's Hospital, Weifang261000, Shandong Province, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Yidu Central Hospital, Weifang Medical University, Linglongshan South Road No.4138, Qingzhou262500, Shandong Province, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.affiliation: Department of Implantology, Shandong Provincial Key Laboratory of Oral Biomedicine, Shandong University School of Stomatology, Wenhua West Road No.44-1, Jinan250012, Shandong Province, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
keywords: Apoptosis; Interleukin-6; Neutrophil; Periodontal ligament stem cells