title: Comparative Modulation of Levels of Oxidative Stress in the Liver of Anti-Tuberculosis Drug Treated Wistar Rats by Vitamin B12, Beta-Carotene, and Spirulina fusiformis: Role of NF-κB, iNOS, IL-6, and IL-10.
abstract: Isoniazid (INH) and Rifampicin (RIF) are known hepatotoxic agents. We compared the efficacy of Spirulina fusiformis and its active components vitamin B12 and beta-carotene in attenuating INH and RIF induced hepatotoxicity. We also tried to elucidate the inflammatory mechanism behind anti-tuberculosis drug induced hepatotoxicity. INH and RIF were administered to Wistar albino rats for 28 days to induce hepatotoxicity. S. fusiformis, vitamin B12, and beta-carotene were co-administered with INH and RIF and their hepatoprotective, antioxidant, and immunomodulatory roles were studied through blood and liver analysis. Changes induced by INH and RIF in antioxidants, cytokines (IL-6 and IL-10) and expression of Nuclear Factor-κB (NF-κB) and Nitric Oxide Synthase (iNOS) were also studied. Supplement treatment caused restoration of liver function parameters to normal levels along with reversal of inflammatory changes in IL-6 and IL-10 levels. Liver PCNA, iNOS, and NF-κB expression were reduced in the supplement treated tissues compared to INH and RIF treated rats as evidenced by immunohistochemistry and quantitative PCR. Correlation of IL-6 levels, PCNA, and iNOS with NF-κB showed its pivotal role in the inflammatory process. Study shows the pivotal role of NF-kB and the equivalence in antioxidant efficacy of vitamin B12 and beta-carotene compared to Spirulina fusiformis. J. Cell. Biochem. 118: 3825-3833, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: School of Bio Science and Technology, VIT University, Vellore 632014, India.
authors.forename: Sherry
authors.initials: S
authors.lastname: Joseph Martin
authors.affiliation: School of Bio Science and Technology, VIT University, Vellore 632014, India.
authors.forename: Sabina
authors.identifier: 0000-0003-2073-5971
authors.initials: S
authors.lastname: Evan Prince
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000009:adverse effects* / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000378:metabolism*; D019587:Dietary Supplements; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D053545:Spirulina; D014805:Vitamin B 12 / Q000494:pharmacology*; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D015850:Interleukin-6; D016328:NF-kappa B; D019207:beta Carotene; D016753:Interleukin-10; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D014805:Vitamin B 12
keywords: BETA-CAROTENE; HEPATOTOXICITY; ISONIAZID; RIFAMPICIN; SPIRULINA FUSIFORMIS; VITAMIN B12