title: A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation.
abstract: With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.
authors.affiliation: School of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China. c_x_p@dicp.ac.cn.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Novel Technology and Materials for Separation and Detection of Biomolecules Group, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: School of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China.
authors.forename: Haowei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China. xuesong@dicp.ac.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
authors.affiliation: School of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China. tianjing@dlpu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D016825:Chlamydomonas reinhardtii / Q000378:metabolism; D005374:Filtration; D058086:Microalgae / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
keywords: Chlamydomonas reinhardtii; FASP; Proteomics; Sample preparation; mFASP