title: Neuroprotective effect of the Chinese medicine Tiantai No. 1 and its molecular mechanism in the senescence-accelerated mouse prone 8.
abstract: Tiantai No. 1, a Chinese medicine predominantly composed of powderedRhizoma Gastrodiae,Radix Ginseng, and Ginkgo leaf at a ratio of 2:1:2 and dissolved in pure water, is neuroprotective in animal models of various cognitive disorders, but its molecular mechanism remains unclear. We administered Tiantai No. 1 intragastrically to senescence-accelerated mouse prone 8 (SAMP8) mice (a model of Alzheimer's disease) at doses of 50, 100 or 150 mg/kg per day for 8 weeks and evaluated their behavior in the Morris water maze and expression of Alzheimer's disease-related proteins in the brain. Tiantai No. 1 shortened the escape latency in the water maze training trials, and increased swimming time in the target quadrant during the spatial probe test, indicating that Tiantai No. 1 improved learning and memory in SAMP8 mice. Immunohistochemistry revealed that Tiantai No. 1 restored the proliferation potential of Ki67-positive cells in the hippocampus. In addition, mice that had received Tiantai No. 1 had fewer astrocytes, and less accumulation of amyloid-beta and phosphorylated tau. These results suggest that Tiantai No. 1 is neuroprotective in the SAMP8 mouse model of Alzheimer's disease and acts by restoring neuronal number and proliferation potential in the hippocampus, decreasing astrocyte infiltration, and reducing the accumulation of amyloid-beta and phosphorylated tau.
authors.affiliation: First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital), Shenzhen, Guangdong Province, China.
authors.forename: Ying-Hong
authors.identifier: 0000-0002-1963-8560
authors.initials: YH
authors.lastname: Li
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Xu-Sheng
authors.initials: XS
authors.lastname: Wang
authors.affiliation: Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital), Shenzhen, Guangdong Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Shenzhen Key Laboratory of Health Sciences and Technology, Graduate School at Shenzhen, Tsinghua University, Beijing, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital), Shenzhen, Guangdong Province, China.
authors.forename: Xiu-Qin
authors.initials: XQ
authors.lastname: Jia
authors.affiliation: First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital), Shenzhen, Guangdong Province, China.
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital), Shenzhen, Guangdong Province, China.
authors.forename: Zheng-Zhi
authors.identifier: 0000-0002-8819-609X
authors.initials: ZZ
authors.lastname: Wu
keywords: Alzheimer's disease; SAMP8; Tiantai No. 1; amyloid-beta; astrocytosis; autophagy-lysosome pathway; nerve regeneration; neural regeneration; neuronal apoptosis; neuroprotective effects; tau phosphorylation; ubiquitin proteasome pathway