title: Low-intensity pulsed ultrasound (LIPUS) prevents periprosthetic inflammatory loosening through FBXL2-TRAF6 ubiquitination pathway.
abstract: Previous studies have shown that Low intensity pulsed ultrasound(LIPUS) prevents polyethylene-debris-induced periprosthetic loosening in vivo, but the details of the mechanism by which it does so remain unclear. In this article, we used polyethylene debris induced RAW 264.7 cells as the in vitro model, and tested the effect of LIPUS on this model. Changes in the level of inflammatory cytokines, cell proliferation, and apoptosis were assessed. Gene overexpression and siRNA technique were applied, and the levels of expression of FBXL2, TRAF6, ERK, and related inflammatory cytokines were also measured. Results indicated that FBXL2-mediated TRAF6 ubiquitination and degradation also plays an important role in aseptic periprosthetic loosening process, and LIPUS prevents such loosening by strengthening this pathway.
authors.affiliation: Department of orthopaedic surgery, the second affiliated hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Institute of orthopaedic research, Zhejiang University, Hangzhou, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedic Surgery, Yiwu Central Hospital, the affiliated hospital of Wenzhou Medical College, Yiwu, China.
authors.forename: Gangsheng
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of orthopaedic surgery, the second affiliated hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Institute of orthopaedic research, Zhejiang University, Hangzhou, China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of orthopaedic surgery, the second affiliated hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Institute of orthopaedic research, Zhejiang University, Hangzhou, China.
authors.forename: Chenhe
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedic Surgery, Ningbo sixth hospital, China.
authors.forename: Yunlin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of orthopaedic surgery, the second affiliated hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Institute of orthopaedic research, Zhejiang University, Hangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of orthopaedic surgery, the second affiliated hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Institute of orthopaedic research, Zhejiang University, Hangzhou, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Yan
mesh_terms: D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D044783:F-Box Proteins / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D020959:Polyethylene / Q000633:toxicity*; D019736:Prostheses and Implants / Q000009:adverse effects*; D000067996:RAW 264.7 Cells; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism*; D054875:Ubiquitination; D000069453:Ultrasonic Waves
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D044783:F-Box Proteins; C558726:FBXL2 protein, mouse; D048029:TNF Receptor-Associated Factor 6; D020959:Polyethylene