title: In VivoImaging Sheds Light on Immune Cell Migration and Function in Cancer.
abstract: There is ample evidence for both beneficial and harmful involvement of the immune system in tumor development and spread. Immune cell recruitment to tumors is essential not only for the success of anticancer immune therapies but also for tumor-induced immune suppression. Now that immune-based therapies are playing an increasingly important role in treatment of solid tumors such as metastatic melanomas, precise analysis of thein vivocontributions of different leukocyte subsets in tumor immunity has become an even greater priority. Recently, this goal has been markedly facilitated by the use of intravital microscopy, which has enabled us to visualize the dynamic interactions between cells of the immune system and tumor targets in the context of the tumor microenvironment. For example, intravital imaging techniques have shed new light on T cell infiltration of tumors, the mechanisms of cancer cell killing, and how myeloid cells contribute to tumor tolerance and spread. This mini-review summarizes the recent advances made to our understanding of the roles of innate and adaptive immune cells in cancer based on the use of thesein vivoimaging approaches.
authors.affiliation: Immunology Division, Garvan Institute of Medical Research, Sydney, NSW, Australia; Faculty of Medicine, St Vincent's Clinical School, University of New South Wales, Sydney, NSW, Australia.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Torcellan
authors.affiliation: Immunology Division, Garvan Institute of Medical Research, Sydney, NSW, Australia; Faculty of Medicine, St Vincent's Clinical School, University of New South Wales, Sydney, NSW, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Stolp
authors.affiliation: Immunology Division, Garvan Institute of Medical Research, Sydney, NSW, Australia; Faculty of Medicine, St Vincent's Clinical School, University of New South Wales, Sydney, NSW, Australia.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Chtanova
keywords: antitumor immune response; cellular dynamics; immune suppression; in vivo imaging; intravital microscopy; migration