title: Fibrin gel as a scaffold for skin substitute – production and clinical experience.
abstract: The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaffolds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed. After hydrosurgical preparation of deep burn necrotic tissue, wound bed was prepared for skin substitutes. Progress of healing was documented using visual estimation and photos. Scanning electron microscope images showed good cell attachment and colony spreading of
  keratinocytes and fibroblasts on fibrin scaff old. Immunofluorescent staining of cell cultures on fibrin scaffold showed expression of vimentin, a marker of fibroblast cells, cytokeratin 19, a marker of epithelial stem cells, as well as involucrin, a marker of differentiated keratinocytes. Clinical results clearly  showed that appearance of the skin did not differ significantly from the areas of transplanted skin using split-thickness skin graft techniques. In conclusion, using these fibrin-cultured autografts on massive full-thickness burn resulted in good healing.
authors.affiliation: Zagreb Children's Hospital, Zagreb, Croatia
authors.forename: Antun
authors.initials: A
authors.lastname: Kljenak
authors.affiliation: Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
authors.forename: Mirna
authors.identifier: 0000-0002-2918-7388
authors.initials: M
authors.lastname: Tominac Trcin
authors.affiliation: Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
authors.forename: Marina
authors.initials: M
authors.lastname: Bujić
authors.affiliation: Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
authors.forename: Tamara
authors.initials: T
authors.lastname: Dolenec
authors.affiliation: Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
authors.forename: Martina
authors.initials: M
authors.lastname: Jevak
authors.affiliation: Ivan Vučetić Center for Forensic Investigations, Research and Expertise, Zagreb, Croatia|Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
authors.forename: Gordan
authors.initials: G
authors.lastname: Mršić
authors.affiliation: Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
authors.forename: Gordana
authors.initials: G
authors.lastname: Zmiš
authors.affiliation: Zagreb Children's Hospital, Zagreb, Croatia
authors.forename: Zoran
authors.identifier: 0000-0001-7123-1429
authors.initials: Z
authors.lastname: Barčot
authors.affiliation: Tissue Bank, University Department of Traumatology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
authors.forename: Ante
authors.initials: A
authors.lastname: Muljačić
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
authors.forename: Maja
authors.initials: M
authors.lastname: Popović
mesh_terms: D000293:Adolescent; D002056:Burns / Q000473:pathology / Q000628:therapy*; D018929:Cell Culture Techniques; D002470:Cell Survival; D002675:Child, Preschool; D005337:Fibrin; D005347:Fibroblasts; D006801:Humans; D015603:Keratinocytes; D008297:Male; D016038:Skin Transplantation; D017768:Skin, Artificial; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D005337:Fibrin
keywords: Cells, cultured; Fibrin; Skin, artificial; Burns