title: Antagonistic Activity ofLactobacillus reuteriStrains on the Adhesion Characteristics of Selected Pathogens.
abstract: Adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. Therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. Presents study documents the antagonistic activity of viable/untreated, Lithium chloride (LiCl) treated or heat-killed forms of eight probioticLactobacillus reuteristrains on the adhesion characteristics of selected pathogens. All strains investigated were able to adhere to Caco-2 cells.L. reuteristrains tested were able to inhibit and displace (P< 0.05) the adhesion ofEscherichia coliATCC25922,Salmonella typhiNCDC113,Listeria monocytogenesATCC53135, andEnterococcus faecalisNCDC115. The probiotic strainL. reuteriLR6 showed the strongest adhesion and pathogen inhibition ability among the eightL. reuteristrains tested. In addition, the abilities to inhibit and to displace adhered pathogens depended on both the probiotic and the pathogen strains tested suggesting the involvement of various mechanisms. The adhesion and antagonistic potential of the probiotic strains were significantly decreased upon exposure to 5 M LiCl, showing that surface molecules, proteinaceous in nature, are involved. The heat-killed forms of the probioticL. reuteristrains also inhibited the attachment of selected pathogens to Caco-2 cells. In conclusion,in vitroassays showed thatL. reuteristrains, as viable or heat-killed forms, are adherent to Caco-2 cells and are highly antagonistic to pathogens tested in which surface associated proteins play an important role.
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute Karnal, India.
authors.forename: Tejinder P
authors.initials: TP
authors.lastname: Singh
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute Karnal, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute Karnal, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Kapila
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute Karnal, India.
authors.forename: Ravinder K
authors.initials: RK
authors.lastname: Malik
keywords: Caco-2 cells; Lactobacillus reuteri; adhesion; antagonistic activity; probiotics