title: Antidepressant-like activity of icariin mediated by group I mGluRs in prenatally stressed offspring.
abstract: OBJECTIVE
  The present study was performed to identify antidepressant-like activity of icariin in prenatally stressed male rats.
  
  
  METHODS
  The effects of icariin on PRS-induced depression were examined using sucrose preference test (SPT) and forced swimming test (FST) in male offspring, and measuring protein and mRNA expressions of group I mGluRs receptors and EAAT2 via western blotting and quantitative real-time PCR assays.
  
  
  RESULTS
  The results indicated that prenatal restraint stress (PRS) resulted in several behavioral anomalies. Treatment with icariin relieved the elevated protein and mRNA levels of group I mGluR receptors as well as the diminished protein and mRNA levels of EAAT2 in the PRS male offspring.
  
  
  CONCLUSIONS
  Collectivity, the data support that icariin ameliorates PRS-induced depressive-like behavior via regulating expression of mGluR1, mGluR5 and EAAT2 in the hippocampus.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 86-710069, PR China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shaanxi Institute of Pediatric Diseases, Affiliated Children's Hospital of Xi'an Jiaotong University, Xi'an 86-710003, PR China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 86-710061, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Su
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Lin
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 86-710061, PR China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 86-710061, PR China.
authors.forename: Shaokang
authors.initials: S
authors.lastname: Dang
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 86-710069, PR China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 86-710069, PR China. Electronic address: zlzhu@mail.xjtu.edu.cn.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D003866:Depressive Disorder / Q000188:drug therapy* / Q000378:metabolism*; D004195:Disease Models, Animal; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D064529:Receptor, Metabotropic Glutamate 5 / Q000378:metabolism; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D012149:Restraint, Physical; D013315:Stress, Psychological
chemical_list: D000928:Antidepressive Agents; D027342:Excitatory Amino Acid Transporter 2; D005419:Flavonoids; C578937:Grm5 protein, rat; D012333:RNA, Messenger; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; C493395:Slc1a2 protein, rat; C104077:metabotropic glutamate receptor type 1; C056599:icariin
keywords: Depression; EAAT2; Group I mGluR receptors; Icariin; Prenatal restraint stress