title: Ca2+Measurements in Mammalian Cells with Aequorin-based Probes.
abstract: Aequorin is a Ca2+sensitive photoprotein suitable to measure intracellular Ca2+transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca2+uptake and release of different intracellular organelles. Here, we describe how to use this probe to measure cytosolic Ca2+levels and mitochondrial Ca2+uptake in mammalian cells.
authors.affiliation: Department of Biomedical Sciences, University of Padua, Padua, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Tosatto
authors.affiliation: Department of Biomedical Sciences, University of Padua, Padua, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Rizzuto
authors.affiliation: Department of Biomedical Sciences, University of Padua, Padua, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Mammucari
keywords: Aequorin; Ca2+; Luminescence; Probes