title: The Arabidopsis Cop9 signalosome subunit 4 (CNS4) is involved in adventitious root formation.
abstract: The COP9 signalosome (CSN) is an evolutionary conserved multiprotein complex that regulates many aspects of plant development by controlling the activity of CULLIN-RING E3 ubiquitin ligases (CRLs). CRLs ubiquitinate and target for proteasomal degradation a vast number of specific substrate proteins involved in many developmental and physiological processes, including light and hormone signaling and cell division. As a consequence of CSN pleiotropic function, complete loss of CSN activity results in seedling lethality. Therefore, a detailed analysis of CSN physiological functions in adult Arabidopsis plants has been hampered by the early seedling lethality of csn null mutants. Here we report the identification and characterization of a viable allele of the Arabidopsis COP9 signalosome subunit 4 (CSN4). The allele, designated csn4-2035, suppresses the adventitious root (AR) phenotype of the Arabidopsis superroot2-1 mutant, potentially by altering its auxin signaling. Furthermore, we show that although the csn4-2035 mutation affects primary and lateral root (LR) formation in the 2035 suppressor mutant, CSN4 and other subunits of the COP9 complex seem to differentially control AR and LR development.
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187, Umeå, Sweden. Daniel.Pacurar@swetree.com.|SweTree Technologies AB, P.O. Box 4095, SE-904 03, Umeå, Sweden. Daniel.Pacurar@swetree.com.
authors.forename: Daniel Ioan
authors.initials: DI
authors.lastname: Pacurar
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187, Umeå, Sweden.|University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj Napoca, Romania.|SweTree Technologies AB, P.O. Box 4095, SE-904 03, Umeå, Sweden.
authors.forename: Monica Lacramioara
authors.initials: ML
authors.lastname: Pacurar
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187, Umeå, Sweden.
authors.forename: Abdellah
authors.initials: A
authors.lastname: Lakehal
authors.affiliation: University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj Napoca, Romania.
authors.forename: Andrea Mariana
authors.initials: AM
authors.lastname: Pacurar
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187, Umeå, Sweden.
authors.forename: Alok
authors.initials: A
authors.lastname: Ranjan
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187, Umeå, Sweden. Catherine.Bellini@umu.se.|Institut National de la Research Agronomic, UMR1318 INRA-AgroParisTech, Institut Jean-Pierre Bourgin, Univ. Paris-Sud, F-78000, Versailles, France. Catherine.Bellini@umu.se.
authors.forename: Catherine
authors.identifier: http://orcid.org/0000-0003-2985-6649
authors.initials: C
authors.lastname: Bellini