title: Postoperative Weight Loss Masks Metabolic Impacts of Periodontitis in Obese Rodents.
abstract: Postoperative weight loss (POWL) is expected to occur in combined models of obesity and periodontitis. This study explores the confounding effects of POWL on the impact of ligation-induced periodontitis on glucose and lipid metabolism in obese animals.
  
  
  
  Combined mouse models of diet-induced obesity (DIO) and ligation-induced periodontitis (5- or 10-day ligation) were studied. Fasting serum glucose (FSG), fasting insulin (Fins), and lipids including triglyceride (TG), total cholesterol (TC), and low- and high-density lipoprotein cholesterol (HDLC), were detected via biochemistry and enzyme-linked immunosorbent assay. POWL and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. Analysis of covariance was performed to identify confounding effects of POWL.
  
  
  
  The obesity, periodontitis, and 10-day groups exhibited greater POWL than corresponding controls (P <0.01). Without considering POWL, conflicting results were found, including: 1) contradictory changes in HDLC caused by obesity or periodontitis; and 2) unequal levels of FSG, TC, and HDLC between days 5 and 10 in the sham-ligation controls. Moreover, upregulating effects of periodontitis were found only on TG in the DIO mice, whereas those on Fins, HOMA-IR, and HDLC were statistically veiled. After the confounding effects of POWL were filtered, periodontitis promoted increased levels of not only TG but also Fins, HOMA-IR, and HDLC in the DIO mice (P <0.05).
  
  
  
  When analyzing the interrelationship between obesity and periodontitis, the confounding effects of an imbalanced POWL should be considered. Otherwise, impact of periodontitis on metabolic dysregulation in obese animals may be underestimated.
authors.affiliation: Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Prosthodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Stomatology, Guangdong Engineering Research Center of Technology and Materials for Oral Reconstruction, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Periodontology, Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Periodontology, Ai Kang Jian Dental Group, Shenzhen, China.
authors.forename: Meilian
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou, China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Periodontology, Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou, China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Periodontology, Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Periodontology, Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou, China.
authors.forename: Yixiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Periodontology, Affiliated Hospital of Stomatology, Southern Medical University, Guangzhou, China.
authors.forename: Baoyi
authors.initials: B
authors.lastname: Xie
authors.affiliation: Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
authors.affiliation: Department of Periodontology, Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Jincai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou, China.
authors.forename: Dongying
authors.initials: D
authors.lastname: Xuan
mesh_terms: D016301:Alveolar Bone Loss; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL / Q000097:blood; D016207:Cytokines / Q000097:blood; D004032:Diet; D004195:Disease Models, Animal; D005215:Fasting; D005260:Female; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000097:blood / Q000150:complications* / Q000378:metabolism*; D010518:Periodontitis / Q000097:blood / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D014280:Triglycerides / Q000097:blood; D015431:Weight Loss
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D016207:Cytokines; D007328:Insulin; D014280:Triglycerides; D002784:Cholesterol; D005947:Glucose
keywords: Dyslipidemias; insulin resistance; metabolism; obesity; periodontal diseases; weight loss