title: Exploring the iceberg: Prospects of coordinated myosin V and actin assembly functions in transport processes.
abstract: Spir actin nucleators and myosin V motor proteins were recently discovered to coexist in a protein complex. The direct interaction allows the coordinated activation of actin motor proteins and actin filament track generation at vesicle membranes. By now the cooperation of myosin V (MyoV) motors and Spir actin nucleation function has only been shown in the exocytic transport of Rab11 vesicles in metaphase mouse oocytes. Next to Rab11, myosin V motors however interact with a variety of Rab GTPases including Rab3, Rab8 and Rab10. As a common theme most of the MyoV interacting Rab GTPases function at different steps along the exocytic transport routes. We here summarize the different transport functions of class V myosins and provide as proof of principle data showing a colocalization of Spir actin nucleators and MyoVa at Rab8a vesicles. This suggests that besides Rab11/MyoV transport also the Rab8/MyoV and possibly other MyoV transport processes recruit Spir actin filament nucleation function.
authors.affiliation: a University Hospital Regensburg, Department of Neurology , Molecular Cell Biology Laboratory , Regensburg , Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Welz
authors.affiliation: a University Hospital Regensburg, Department of Neurology , Molecular Cell Biology Laboratory , Regensburg , Germany.
authors.forename: Eugen
authors.initials: E
authors.lastname: Kerkhoff
mesh_terms: D000199:Actins / Q000378:metabolism*; D001692:Biological Transport; D006801:Humans; D024701:Myosin Type V / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000199:Actins; D024701:Myosin Type V; D020741:rho GTP-Binding Proteins
keywords: Rab GTPases; Spir; actin nucleation; formin; myosin V; myosin actin motor proteins; vesicle transport