title: Cellular Prion Protein PrPCand Ecto-5'-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy.
abstract: Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrPCand the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73+cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrPCwas observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells.Cancer Res; 77(11); 2914-26. ©2017 AACR.
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors.forename: Patrícia H
authors.initials: PH
authors.lastname: Domingues
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.|Amrita Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India.
authors.forename: Lalitha S Y
authors.initials: LSY
authors.lastname: Nanduri
authors.affiliation: Group Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Martinsried, Germany.|Department of Molecular Genetics, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Seget
authors.affiliation: Division of Molecular Thoracic Oncology, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany.
authors.forename: Sharavan V
authors.initials: SV
authors.lastname: Venkateswaran
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Agorku
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Cristina
authors.initials: C
authors.lastname: Viganó
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Conrad
authors.initials: C
authors.lastname: von Schubert
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Erich A
authors.initials: EA
authors.lastname: Nigg
authors.affiliation: Translational Cancer Therapeutics Laboratory, Francis Crick Institute, London, United Kingdom.
authors.forename: Charles
authors.initials: C
authors.lastname: Swanton
authors.affiliation: Division of Molecular Thoracic Oncology, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany.
authors.forename: Rocío
authors.initials: R
authors.lastname: Sotillo
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bosio
authors.affiliation: Group Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Martinsried, Germany.|Department of Molecular Genetics, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Storchová
authors.affiliation: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. olaf.hardt@miltenyibiotec.de.
authors.forename: Olaf
authors.initials: O
authors.lastname: Hardt
mesh_terms: D015720:5'-Nucleotidase / Q000096:biosynthesis / Q000378:metabolism*; D000782:Aneuploidy; D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D000072002:Prion Proteins / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D000072002:Prion Proteins; D015720:5'-Nucleotidase