title: Overexpression of microRNA-196b Accelerates Invasiveness of Cancer Cells in Recurrent Epithelial Ovarian Cancer Through Regulation of Homeobox A9.
abstract: Although microRNAs (miRNAs) are known to influence messenger RNA post-transcriptional control and contribute to human tumorigenesis, little is known about the differences in miRNA expression between primary and recurrent epithelial ovarian cancer (EOC). The purpose of this study was to assess the differential miRNA expression between primary and recurrent EOC and to investigate whether miR-196b could regulate the expression of the Homeobox A9 (HOXA9) gene, and thus affect the invasiveness of cancer cells in recurrent EOC.
  
  
  
  Microarrays were used to generate the expression profiles of 6658 miRNAs from samples of 10 patients with EOC. miRNA expression patterns were compared between primary and recurrent EOC. Aberrantly expressed miRNA, associated genes, and invasion activities were validated by a luciferase assay and an in vitro invasion assay.
  
  
  
  miRNA microarray analysis identified 33 overexpressed miRNAs (including miR-196b) and 18 under expressed miRNAs in recurrent EOC from 6658 human miRNAs. HOXA9 expression was inversely correlated with miR-196b levels in recurrent EOC. We noted that miR-196b induced ovarian cancer cell invasiveness in recurrent EOC by an in vitro invasion assay.
  
  
  
  Overexpression of miR-196b may contribute to invasion activities in recurrent EOC by regulating the HOXA9 gene. Moreover, miR-196b can be a potential biomarker in recurrent EOC.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Gun Oh
authors.initials: GO
authors.lastname: Chong
authors.affiliation: Mmonitor. Inc., Daegu, Republic of Korea ylchoknuh@naver.com jeonh@mmonitorings.com.
authors.forename: Hyo-Sung
authors.initials: HS
authors.lastname: Jeon
authors.affiliation: Department of Physiology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Han
authors.affiliation: Department of Internal Medicine, Konyang University Hospital, Daejeon, Republic of Korea.
authors.forename: Ji Woong
authors.initials: JW
authors.lastname: Son
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Dae Gy
authors.initials: DG
authors.lastname: Hong
authors.affiliation: Mmonitor. Inc., Daegu, Republic of Korea.
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Yoon Soon
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea ylchoknuh@naver.com jeonh@mmonitorings.com.
authors.forename: Young Lae
authors.initials: YL
authors.lastname: Cho
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; D018398:Homeodomain Proteins; C542543:MIRN196 microRNA, human; D035683:MicroRNAs; C097899:homeobox protein HOXA9
keywords: Epithelial ovarian cancer; HOXA9 gene; invasiveness; miR-196b; recurrence