title: Nutrients and COD removal of swine wastewater with an isolated microalgal strain Neochloris aquatica CL-M1 accumulating high carbohydrate content used for biobutanol production.
abstract: In this study, a carbohydrate-rich microalga Neochloris aquatica CL-M1 was adapted to grow in swine wastewater. The effects of cultivation conditions (i.e., temperature, light intensity or N/P ratio) on COD/nutrients removal and carbohydrate-rich biomass production were investigated. The results indicate that the highest COD removal (81.7%) and NH3-N removal (96.2%) was achieved at 150µmolm-2s-1light intensity, 25°C and N/P ratio=1.5/1. The highest biomass concentration and carbohydrate content was 6.10gL-1and 50.46%, respectively, when N/P ratio=5/1. The resulting carbohydrate-rich microalgal biomass was pretreated and used as a feedstock for butanol fermentation. With the initial sugar concentration of 48.7gL-1glucose and 3.4gL-1xylose in the pretreated biomass, the butanol concentration, yield, and productivity were 12.0gL-1, 0.60molmol-1sugar, and 0.89gL-1h-1, respectively, indicating the high potential of using Neochloris aquatica CL-M1 for butanol fermentation.
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chieh-Lun
authors.initials: CL
authors.lastname: Cheng
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Dillirani
authors.initials: D
authors.lastname: Nagarajan
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Wan-Qian
authors.initials: WQ
authors.lastname: Guo
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chiayi
authors.initials: C
authors.lastname: Lin
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China.
authors.forename: Shuangfei
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D000818:Animals; D018533:Biomass; D002241:Carbohydrates; D000460:Chlorophyta; D058086:Microalgae; D013552:Swine; D062065:Wastewater
chemical_list: D002241:Carbohydrates; D062065:Wastewater
keywords: Butanol fermentation; COD/nutrient removal; Carbohydrate; Microalgae; Neochloris aquatica CL-M1; Swine wastewater