title: Lipid Layers on Nanoscale Surface Topography: Stability and Effect on Protein Adsorption.
abstract: Herein, we report the coating of a surface with a random nanoscale topography with a lipid film formed by an anchoring stearic acid (SA) monolayer and phospholipid (DPPC) layers. For this purpose, different procedures were used for phospholipid coating, including adsorption from solution, drop deposition, and spin-coating. Our results reveal that the morphology of the obtained lipid films is strongly influenced by the topography of the underlying substrate but also impacted by other factors, including the coating procedure and surface wettability (modulated by the presence of SA). These coated surfaces showed a remarkable antifouling behavior toward proteins, with different yields of repellency (Yrp) depending on the amount/organization of DPPC on the nanostructured substrate. The interaction between the proteins and phospholipids involves a partial detachement of the film. The use of characterization techniques with different charcateristics (accuracy, selectivity, analysis depth) did not reveal any obvious vertical heterogenity of the probed interface, indicating that the lipid film acts as a nonfouling coating on the whole surface, including the outermost part (nanoprotrusions) and deeper regions (valleys).
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 , F-75005 Paris, France.|CNRS, UMR 7197 , Laboratoire de Réactivité de Surface, F-75005 Paris, France.|State Research Institute Center for Physical Sciences and Technology , Saulėtekio av. 3, LT-10257 Vilnius, Lithuania.
authors.forename: Irma
authors.initials: I
authors.lastname: Liascukiene
authors.affiliation: Laboratoire de Biomécanique & Bioingénierie, CNRS, UMR 7338, Université de Technologie de Compiègne , BP 20529, F-60205 Compiègne Cedex, France.
authors.forename: Karim
authors.initials: K
authors.lastname: El Kirat
authors.affiliation: Alcatel-Lucent Bell Labs France , 7 route de Villejust, 91620 Nozay, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Beauvais
authors.affiliation: State Research Institute Center for Physical Sciences and Technology , Saulėtekio av. 3, LT-10257 Vilnius, Lithuania.
authors.forename: Svajus J
authors.initials: SJ
authors.lastname: Asadauskas
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 , F-75005 Paris, France.|CNRS, UMR 7197 , Laboratoire de Réactivité de Surface, F-75005 Paris, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Lambert
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06 , F-75005 Paris, France.|CNRS, UMR 7197 , Laboratoire de Réactivité de Surface, F-75005 Paris, France.
authors.forename: Jessem
authors.identifier: 0000-0003-2394-4437
authors.initials: J
authors.lastname: Landoulsi
mesh_terms: D000327:Adsorption; D049329:Nanostructures; D010743:Phospholipids; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D010743:Phospholipids; D011506:Proteins