title: Repellent and Larvicidal Activity of the Essential Oil From Eucalyptus nitens Against Aedes aegypti and Aedes albopictus (Diptera: Culicidae).
abstract: Dengue, chikungunya, and yellow fever are important vector-borne diseases transmitted by female mosquitoes when they feed on humans. The use of repellents based on natural products is an alternative for personal protection against these diseases. Application of chemicals with larvicidal activity is another strategy for controlling the mosquito population. The repellent and larvicidal activities of the essential oil from Eucalyptus nitens were tested against Aedes aegypti and Aedes albopictus, the main vectors of these arboviruses. The essential oil was extracted by hydrodistillation and then analyzed by gas chromatography-mass spectrometry. The main components of Eucalyptus nitens essential oil were found to be terpenes such as 1,8-cineole and p-cymene, followed by β-triketones and alkyl esters. The repellent activity of the essential oil against both species was significantly higher when compared with the main component, 1,8-cineole, alone. These results indicate that the repellent effect of E. nitens is not due only to the main component, 1,8-cineole, but also that other compounds may be responsible. Aedes aegypti was found to be more tolerant to the essential oil larvicidal effects than Ae. albopictus (Ae. aegypti LC50 = 52.83 ppm, Ae. albopictus LC 50 = 28.19 ppm). The repellent and larvicidal activity could be associated to the presence of cyclic β-triketones such as flavesone, leptospermone, and isoleptospermone.
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF-CONICET), Juan Bautista de La Salle 4397, B1603ALO, Villa Martelli, Buenos Aires, Argentina (aalvarezcosta@citedef.gob.ar; cnaspi@citedef.gob.ar; alucia@citedef.gob.ar; hmasuh@citedef.gob.ar).
authors.forename: Agustín
authors.initials: A
authors.lastname: Alvarez Costa
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF-CONICET), Juan Bautista de La Salle 4397, B1603ALO, Villa Martelli, Buenos Aires, Argentina (aalvarezcosta@citedef.gob.ar; cnaspi@citedef.gob.ar; alucia@citedef.gob.ar; hmasuh@citedef.gob.ar).
authors.forename: Cecilia V
authors.initials: CV
authors.lastname: Naspi
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF-CONICET), Juan Bautista de La Salle 4397, B1603ALO, Villa Martelli, Buenos Aires, Argentina (aalvarezcosta@citedef.gob.ar; cnaspi@citedef.gob.ar; alucia@citedef.gob.ar; hmasuh@citedef.gob.ar).
authors.forename: Alejandro
authors.initials: A
authors.lastname: Lucia
authors.affiliation: Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-UNIDEF-CONICET), Juan Bautista de La Salle 4397, B1603ALO, Villa Martelli, Buenos Aires, Argentina (aalvarezcosta@citedef.gob.ar; cnaspi@citedef.gob.ar; alucia@citedef.gob.ar; hmasuh@citedef.gob.ar).
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Masuh
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D005052:Eucalyptus / Q000033:anatomy & histology / Q000737:chemistry* / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D007302:Insect Repellents / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009822:Oils, Volatile / Q000032:analysis / Q000494:pharmacology*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000737:chemistry
chemical_list: D007302:Insect Repellents; D007306:Insecticides; D009822:Oils, Volatile
keywords: Aedes aegypti; Aedes albopictus; Eucalyptus nitens; larvicidal; repellent