title: Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots.
abstract: Meiotic homologous recombination, a critical event for ensuring faithful chromosome segregation and creating genetic diversity, is initiated by programmed DNA double-strand breaks (DSBs) formed at recombination hotspots. Meiotic DSB formation is likely to be influenced by other DNA-templated processes including transcription, but how DSB formation and transcription interact with each other has not been understood well. In this study, we used fission yeast to investigate a possible interplay of these two events. A group of hotspots in fission yeast are associated with sequences similar to the cyclic AMP response element and activated by the ATF/CREB family transcription factor dimer Atf1-Pcr1. We first focused on one of those hotspots,ade6-3049, and Atf1. Our results showed that multiple transcripts, shorter than theade6full-length messenger RNA, emanate from a region surrounding theade6-3049hotspot. Interestingly, we found that the previously known recombination-activation region of Atf1 is also a transactivation domain, whose deletion affected DSB formation and short transcript production atade6-3049These results point to a possibility that the two events may be related to each other atade6-3049In fact, comparison of published maps of meiotic transcripts and hotspots suggested that hotspots are very often located close to meiotically transcribed regions. These observations therefore propose that meiotic DSB formation in fission yeast may be connected to transcription of surrounding regions.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 153-8902, Japan.|Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 113-0032, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 153-8902, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Okamura
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 153-8902, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Oda
authors.affiliation: Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Tokyo 112-8551, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 153-8902, Japan.|Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 113-0032, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Ohta
authors.affiliation: Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Tokyo 112-8551, Japan tyamada@bio.chuo-u.ac.jp.
authors.forename: Takatomi
authors.initials: T
authors.lastname: Yamada
mesh_terms: D051697:Activating Transcription Factor 1 / Q000235:genetics*; D051696:Activating Transcription Factors / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein; D053903:DNA Breaks, Double-Stranded; D059765:Homologous Recombination / Q000235:genetics; D008540:Meiosis / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics*; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*
chemical_list: D051697:Activating Transcription Factor 1; D051696:Activating Transcription Factors; D017362:Cyclic AMP Response Element-Binding Protein; C097701:Pcr1 protein, S pombe; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; C106740:atf1 protein, S pombe
keywords: DNA double-strand break formation; chromatin; meiosis; meiotic recombination; transcription