title: Effects of exogenous short-chain N-acyl homoserine lactone on denitrifying process of Paracoccus denitrificans.
abstract: N-acyl-homoserine lactones (AHLs) serve as quorum-sensing signals, which control a number of bacterial processes in many proteobacteria. Here we report the effects of exogenous short-chain AHL on the denitrifying process of Paracoccus denitrificans, which are capable of aerobic and anaerobic growth by utilizing nitrate. The denitrification activity of these cells was monitored by measuring denitrification products (including nitrate, nitrite, and nitrous oxide), and the individual messenger ribonucleic acid (mRNA) levels of nitrate, nitrite, nitric oxide and nitrous oxide reductases. The results indicated that 2μmol/L C6-homoserine lactone (HSL) has little effect on cell density under either anaerobic or aerobic culture conditions, and the nitrate reduction activity appeared slightly affected by N-hexanoyl-DL-homoserine lactone (C6-HSL). However, exogenous C6-HSL significantly affected the transcription of nitrite reductase and nitric oxide reductase genes in P. denitrificans regardless of the presence of oxygen, and N2O accumulation activity in P. denitrificans was suppressed by C6-HSL under aerobic condition. In contrast, exogenous C6-HSL stimulated the production of N2O under anaerobic condition, suggesting that the regulation of denitrification by quorum sensing may be important in N2O release.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei 230026, China; CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: janeyun0919@126.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Qiuxuan
authors.initials: Q
authors.lastname: Shen
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: jiegao@rcees.ac.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xlzhuang@rcees.ac.cn.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhuang
mesh_terms: D015107:4-Butyrolactone / Q000378:metabolism; D054742:Acyl-Butyrolactones / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D058440:Denitrification / Q000502:physiology*; D010088:Oxidoreductases / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000502:physiology*; D053038:Quorum Sensing
chemical_list: D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D010088:Oxidoreductases; C022763:nitrous oxide reductase; C034747:nitric-oxide reductase; D015107:4-Butyrolactone
keywords: Denitrification activity; N-acyl-homoserine lactones; Quorum sensing; Transcriptional regulation