title: Different inoculum loads of Toxoplasma gondii induce reduction of myenteric neurons of the rat colon.
abstract: Toxoplasmosis, a disease caused by Toxoplasma gondii, is an important health problem, especially in immunocompromised hosts. T. gondii uses the gut wall as an infection gateway, with tropism for muscular and nervous tissues causing intestinal alterations, including some in the enteric nervous system. This study aims at investigating the colon of rats infected by T. gondii in order to understand how the amount of oocysts influences in myenteric neuronal changes. Sixty Wistar rats (Rattus norvegicus) were divided into six groups. One group remained as a control and the others received inocula of 10, 50, 100, 500 or 5,000 oocysts of T. gondii. The animals were euthanized after 30 days of infection. The total neuronal population and the nitrergic subpopulation in the colon myenteric plexus of each animal was counted. The data were statistically analyzed showing less weight gain in rats with 10, 500 and 5,000 oocysts. A decrease in the number of total neurons with 50, 100 or 5,000 oocysts and an increase in the nitrergic population with 10, 100, 500 or 5,000 oocysts were verified. These results show that neuronal alterations are more significant when the infection is induced by larger inocula and reinforces the suspicion that neuronal loss is directed at cholinergic neurons.
authors.affiliation: Programa de Pós-graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá - UEM, Maringá, PR, Brasil.
authors.forename: Rafael Isolani
authors.initials: RI
authors.lastname: Ferezin
authors.affiliation: Programa de Pós-graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá - UEM, Maringá, PR, Brasil.
authors.forename: Suellen Lais
authors.initials: SL
authors.lastname: Vicentino-Vieira
authors.affiliation: Programa de Pós-graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá - UEM, Maringá, PR, Brasil.
authors.forename: Marcelo Biondaro
authors.initials: MB
authors.lastname: Góis
authors.affiliation: Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: Eduardo José de Almeida
authors.initials: EJ
authors.lastname: Araújo
authors.affiliation: Programa de Pós-graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá - UEM, Maringá, PR, Brasil.
authors.forename: Gessilda de Alcântara Nogueira de
authors.initials: GA
authors.lastname: Melo
authors.affiliation: Laboratório de Doenças Infecciosas, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.affiliation: Programa de Pós-graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá - UEM, Maringá, PR, Brasil.
authors.forename: Débora de Mello Gonçales
authors.initials: DM
authors.lastname: Sant'Ana
mesh_terms: D000818:Animals; D003106:Colon / Q000294:innervation / Q000469:parasitology*; D009474:Neurons / Q000469:parasitology* / Q000473:pathology; D010270:Parasite Egg Count / Q000662:veterinary; D051381:Rats; D017208:Rats, Wistar; D014122:Toxoplasma; D014124:Toxoplasmosis, Animal / Q000150:complications*