title: NDV-D90 suppresses growth of gastric cancer and cancer-related vascularization.
abstract: Recent reports suggest promises on using oncolytic Newcastle disease viruses (NDV) to treat different cancers, while the effects of a NDV-D90 strain on gastric cancer remain unknown. Here we showed that NDV-D90 induced gastric cancer cell apoptosis in a dose-dependent manner in 3 gastric cancer cell lines BGC-823, SGC-7901 and MKN-28. Pronounced reduction in cell invasion was detected in NDV-D90-treated BGC-823 and SGC-7901 cells, but not in MKN-28 cells. The increases in cell apoptosis and reduction in cell growth in NDV-D90-treated gastric cancer cells seemingly resulted from augmentation of p38 signaling and suppression of ERK1/2 and Akt signaling. In vivo, orthotopic injection of NDV-D90 impaired tumor growth and induced intratumoral necrosis. Tumor cells that had been pre-treated with NDV-D90 showed defect in development of implanted tumor. Moreover, NDV-D90 appeared to reduce gastric tumor vascularization, possibly through suppression of vascular endothelial growth factor A and Matrix Metallopeptidase 2. Together, our data suggest that NDV-D90 may have potential anti-cancer effects on gastric cancer.
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sui
authors.affiliation: Department of Intervention, The Second Hospital Affiliated Harbin Medical University, Harbin 150086, China.
authors.forename: Kaibing
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xie
authors.affiliation: Division of Swine Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Medicine, Harbin 150069, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: Division of Swine Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Medicine, Harbin 150069, China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Abdominal Surgery, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Xishan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Intervention, The Second Hospital Affiliated Harbin Medical University, Harbin 150086, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Bai
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Jiazhuang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Radiology, The Fourth Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Shen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000628:therapy*; D009522:Newcastle disease virus / Q000254:growth & development*; D050130:Oncolytic Virotherapy / Q000379:methods*; D013274:Stomach Neoplasms / Q000098:blood supply / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
keywords: apoptosis; gastric cancer; matrix metallopeptidase 2 (MMP-2); newcastle disease viruses (NDV); vascular endothelial growth factor A (VEGF-A)