title: Quantitative evaluation of renal parenchymal perfusion using contrast-enhanced ultrasonography in renal ischemia-reperfusion injury in dogs.
abstract: This study evaluated whether renal perfusion changes can be noninvasively estimated by using contrast-enhanced ultrasonography (CEUS) in renal ischemia-reperfusion injury and investigated the correlation between renal perfusion measured by CEUS and necrosis and apoptosis of renal tubular epithelial cells. In six dogs with experimentally induced renal ischemia-reperfusion injury, changes in time to peak intensity, peak intensity, and area under the curve were measured on CEUS. Peak intensity and area under the curve of the renal cortex began to decrease on day 1 (about 20% lower than baseline) and reached the lowest levels (about 50% of baseline) on day 4. They then gradually increased until day 10, at which time peak intensity was about 87% and area under the curve was about 95% of baseline; neither fully recovered. Both parameters were strongly correlated with the necrosis scores on histopathologic examination on day 4 (r= -0.810 of peak intensity andr= -0.886 of area under the curve). CEUS allowed quantitative evaluation of perfusion changes in acute renal ischemia-reperfusion injury, and CEUS results were correlated with renal tubular damage on histopathologic examination. Thus, CEUS could be a noninvasive, quantitative diagnostic method for determining progress of renal ischemia-reperfusion injury.
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Gahyun
authors.initials: G
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Jeon
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Byunggyu
authors.initials: B
authors.lastname: Cheon
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Sohyeon
authors.initials: S
authors.lastname: Moon
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Jun-Gyu
authors.initials: JG
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
authors.affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Choi
mesh_terms: D000818:Animals; D017209:Apoptosis; D003287:Contrast Media / Q000737:chemistry; D004283:Dog Diseases / Q000000981:diagnostic imaging*; D004285:Dogs; D007672:Kidney Cortex / Q000000981:diagnostic imaging*; D007679:Kidney Medulla / Q000000981:diagnostic imaging*; D007684:Kidney Tubules / Q000000981:diagnostic imaging; D008297:Male; D009336:Necrosis / Q000000981:diagnostic imaging / Q000662:veterinary; D000072356:Parenchymal Tissue / Q000000981:diagnostic imaging*; D015427:Reperfusion Injury / Q000000981:diagnostic imaging / Q000662:veterinary*; D014463:Ultrasonography / Q000662:veterinary*
chemical_list: D003287:Contrast Media
keywords: contrast-enhanced ultrasonography; dogs; renal tubular damage; reperfusion injury; time-intensity curve