title: Protective effect of geraniin against carbon tetrachloride induced acute hepatotoxicity in Swiss albino mice.
abstract: Geraniin is a hydrolysable tannin, widely present in many plant species, specifically used in traditional medicines. It has been shown to exhibit strong antioxidant activity in vitro. This study was performed to investigate hepatoprotective activity of geraniin against carbon tetrachloride (CCl4) induced damage in Swiss albino mice. Mice were treated with 30 and 60 mg/kg geraniin for 10 days followed by CCl4administration for 24 h. Increase in Serum biochemical marker enzymes and histological deteriorative changes of liver tissue after CCl4administration were attenuated by geraniin. Geraniin significantly reduced CCl4induced lipid peroxidation, increase in amount of glutathione, glutathione reductase and Heme oxygenase-1 (HO-1). On the other hand it inhibited significant reduction in catalase activity and expression caused by CCl4administration. Pre-treatment with geraniin reduced phosphorylation of translation initiation factor eIF2α, at serine 51, caused by CCl4exposure and reduced elevated expression of its upstream kinase, Heme-regulated Inhibitor (HRI). These results clearly demonstrate hepatoprotective activity of geraniin against CCl4-induced acute hepatotoxicity via its free radical scavenging and antioxidant activities.
authors.affiliation: Department of Zoology, Savitribai Phule Pune University, Pune 411007, India.
authors.forename: Hoda
authors.initials: H
authors.lastname: Aayadi
authors.affiliation: Department of Zoology, Savitribai Phule Pune University, Pune 411007, India.
authors.forename: Smriti P K
authors.initials: SPK
authors.lastname: Mittal
authors.affiliation: AJ Organica Pvt. Ltd., Pune 411057, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Deshpande
authors.affiliation: AJ Organica Pvt. Ltd., Pune 411057, India.
authors.forename: Makarand
authors.initials: M
authors.lastname: Gore
authors.affiliation: Department of Zoology, Savitribai Phule Pune University, Pune 411007, India. Electronic address: ssg@unipune.ac.in.
authors.forename: Saroj S
authors.initials: SS
authors.lastname: Ghaskadbi
mesh_terms: D016115:Albinism, Oculocutaneous; D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005960:Glucosides / Q000008:administration & dosage*; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D047348:Hydrolyzable Tannins / Q000008:administration & dosage*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D005960:Glucosides; D047348:Hydrolyzable Tannins; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; C024603:Geraniin; D002251:Carbon Tetrachloride; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D017346:Protein Serine-Threonine Kinases; C531557:eIF2alpha kinase, mouse
keywords: CCl(4); Geraniin; HRI; Heme oxygenase-1; eIF2α