title: Modulation of antibiotic activity by the hydroalcoholic extract from leaves of Caryocar coriaceum WITTM.
abstract: The aim of this study was to identify the presence of tannins, phenols and flavonoids on the hydroalcoholic extract of Caryocar coriaceum leaves (HECCL) and to determine the antioxidant and antibacterial activity of this extract. The extract was tested alone (1024-1 μg/mL) or associated (MIC/8) with several antibiotics in order to identify any antibacterial activity against multiresistant bacterial strains (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa). The existence of tannins, total phenols (901.31 mg/g) and flavonoids (89.68 mg/g) was confirmed in the HECCL. The presence of rutin and quercetin were confirmed by Thin-layer chromatography (TLC). Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, the antioxidant activity of the extract (9 μg/mL) was determined. Moreover, the Minimum Inhibitory Concentration (MIC) value found for HECCL was 1024 μg/mL and the association between HECCL (MIC/8) with benzylpenicillin significantly changed its minimum inhibitory concentration from 2500 to 625 μg/mL against E. coli.
authors.affiliation: a Departamento de Química Biológica , Universidade Regional do Cariri - URCA , Crato , Brasil.|b Unidade Acadêmica Ciências da Vida , Universidade Federal de Campina Grande , Cajazeiras , Brasil.
authors.forename: Luís J
authors.initials: LJ
authors.lastname: de Lacerda Neto
authors.affiliation: a Departamento de Química Biológica , Universidade Regional do Cariri - URCA , Crato , Brasil.
authors.forename: A G B
authors.initials: AGB
authors.lastname: Ramos
authors.affiliation: a Departamento de Química Biológica , Universidade Regional do Cariri - URCA , Crato , Brasil.
authors.forename: M R
authors.initials: MR
authors.lastname: Kerntopf
authors.affiliation: a Departamento de Química Biológica , Universidade Regional do Cariri - URCA , Crato , Brasil.
authors.forename: H D M
authors.identifier: http://orcid.org/0000-0002-6634-4207
authors.initials: HDM
authors.lastname: Coutinho
authors.affiliation: c Departamento de Fisiologia , Universidade Federal de Sergipe , São Cristovão , Brasil.
authors.forename: L J
authors.identifier: http://orcid.org/0000-0003-1065-9581
authors.initials: LJ
authors.lastname: Quintans-Junior
authors.affiliation: d Departamento de Ciências Farmacêuticas , Universidade Federal do Vale do São Francisco , Petrolina , Brasil.
authors.forename: J R G S
authors.identifier: http://orcid.org/0000-0001-5155-938X
authors.initials: JRGS
authors.lastname: Almeida
authors.affiliation: e Departamento de Biomedicina , Centro Universitário Dr. Leão Sampaio - UNILEAO , Juazeiro do Norte , Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Ribeiro-Filho
authors.affiliation: a Departamento de Química Biológica , Universidade Regional do Cariri - URCA , Crato , Brasil.
authors.forename: I R A
authors.initials: IRA
authors.lastname: Menezes
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004357:Drug Synergism; D020448:Ericales / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis; D013211:Staphylococcus aureus / Q000187:drug effects; D013634:Tannins / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D012431:Rutin; D011794:Quercetin
keywords: Antibiotic; antibiotic modifying activity; pequi; synergism