title: Comparison of two sedation protocols for short electroretinography in cats.
abstract: Objectives The objectives were to compare two different sedative combinations, xylazine-ketamine and dexmedetomidine-ketamine, for the short electroretinography (ERG) protocol and their impact on sedative effect, reversal times and physiological variables in cats. Methods Six healthy spayed female domestic cats were sedated using one of two ketamine-containing protocols: intramuscular xylazine hydrochloride (1 mg/kg) plus ketamine hydrochloride (3 mg/kg) (XK), and dexmedetomidine hydrochloride (5 µg/kg) plus ketamine hydrochloride (3 mg/kg) (DK). A short ERG protocol was recorded from the left eye of each cat under XK and DK sedation. Thirty minutes later, the effects were reversed with yohimbine or atipamezole for the XK and DK treatment, respectively. The cats were evaluated for time to recumbency, time to head elevation, and time to standing position after reversal treatments. Other variables recorded were: systolic blood pressure, cardiac rhythm, heart rate, pulse oximetry and respiratory rate. Recorded ERG variables included a- and b-wave amplitudes and implicit times under photopic, scotopic and scotopic mixed ERG conditions. Results Time to lateral recumbency with XK was shorter than for DK ( P <0.05). After reversal, head elevation and standing position times were significantly longer for the XK than the DK group ( P <0.05). Heart rate increased and systolic blood pressure decreased from baseline in both groups ( P <0.05), but there were no significant differences between treatment groups. The b-wave amplitude recorded in the photopic study of cats treated with XK was lower than in animals treated with DK ( P <0.05). No other significant differences in ERG variables were observed between treatment groups ( P >0.05). Conclusions and relevance The present study shows that XK and DK treatments are chemical restraint alternatives for ERG recording in cats, with significant differences only in the photopic b-wave amplitude.
authors.affiliation: 1 Laboratory of Pharmacology, Tandil Center of Veterinary Investigation (CIVETAN, CONICET-CICPBA), Faculty of Veterinary Science, National University of the Center of Buenos Aires Province, University Campus, Tandil, Argentina.|2 Teaching Hospital, Faculty of Veterinary Science, National University of the Center of Buenos Aires Province, University Campus, Tandil, Argentina.
authors.forename: María J
authors.initials: MJ
authors.lastname: Del Sole
authors.affiliation: 2 Teaching Hospital, Faculty of Veterinary Science, National University of the Center of Buenos Aires Province, University Campus, Tandil, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Nejamkin
authors.affiliation: 3 Endocrinology Laboratory, Department of Pathophysiology, Faculty of Veterinary Science, National University of the Center of Buenos Aires Province, University Campus, Tandil, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: Cavilla
authors.affiliation: 4 National Council of Scientific and Technical Research, CONICET, Buenos Aires, Argentina.|5 Unit of Clinical Pharmacokinetics, Juan Pedro Garrahan Pediatric Hospital, Buenos Aires, Argentina.
authors.forename: Paula
authors.initials: P
authors.lastname: Schaiquevich
authors.affiliation: 1 Laboratory of Pharmacology, Tandil Center of Veterinary Investigation (CIVETAN, CONICET-CICPBA), Faculty of Veterinary Science, National University of the Center of Buenos Aires Province, University Campus, Tandil, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Moreno
mesh_terms: D000758:Anesthesia / Q000379:methods / Q000662:veterinary*; D000818:Animals; D002415:Cats; D020927:Dexmedetomidine / Q000008:administration & dosage*; D004596:Electroretinography / Q000662:veterinary*; D005260:Female; D006993:Hypnotics and Sedatives / Q000008:administration & dosage*; D007649:Ketamine / Q000008:administration & dosage*; D014991:Xylazine / Q000008:administration & dosage*
chemical_list: D006993:Hypnotics and Sedatives; D014991:Xylazine; D020927:Dexmedetomidine; D007649:Ketamine