title: Effect of combined administration of aripiprazole and fluoxetine on cognitive functions in female rats exposed to ethyl alcohol.
abstract: Alcoholism is a chronic and recurrent disease. The studies on ethyl alcohol show a progressive deterioration of cognitive functions (motor hyperactivity, operating memory). The aim of the study was to establish whether combined single and chronic administration of aripiprazole (ARI) and fluoxetine (FLU) affects animal locomotor activity or modifies spatial memory functions in female rats exposed to ethyl alcohol. Female Wistar rats were studied in the Morris Water Maze (MWM) and locomotor activity test. Rats undergoing the MWM and locomotor activity test were injected with saline on day 1, 7, 14 and 21 of testing. Results showed a statistically significant mobility increase in the group of ethanol‑exposed females (CEt) (21 days) compared to the non-ethanol-exposed group (CNEt). Upon ARI administration to CEt, no statistically significant differences in animal mobility were found, either upon single or chronic administration. Chronic administration of FLU (21 days) as well as combined administration of ARI+FLU (14 and 21 days) caused a statistically significant reduction of the females' mobility compared to the control CEt group. Single and chronic administration of ARI (7x) both show a spatial memory improvement in CEt. No memory improvement was observed, however, after 14 and 21 days of ARI administration. FLU, likewise, improved spatial memory both upon single and chronic administration. Combined administration of ARI+FLU improved memory in CEt only upon single administration. Lack of effect upon chronic administration may be due to tolerance to memory improvement developing upon combined administration of ARI+FLU. It can be concluded that ARI (1.5 mg/kg), FLU (5 mg/kg), and combined administration of these drugs improves spatial memory in CEt.
authors.affiliation: Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Poznan, Poland, kkus@ump.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kus
authors.affiliation: Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ratajczak
authors.affiliation: Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Natasza
authors.initials: N
authors.lastname: Czaja
authors.affiliation: Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zaprutko
authors.affiliation: Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Nowakowska
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000627:therapeutic use*; D000068180:Aripiprazole / Q000627:therapeutic use*; D001362:Avoidance Learning / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000633:toxicity; D003072:Cognition Disorders / Q000188:drug therapy* / Q000209:etiology*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D000431:Ethanol / Q000633:toxicity; D005260:Female; D005473:Fluoxetine / Q000627:therapeutic use*; D008124:Locomotion / Q000187:drug effects; D018782:Maze Learning / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D013997:Time Factors
chemical_list: D014150:Antipsychotic Agents; D002492:Central Nervous System Depressants; D005473:Fluoxetine; D000431:Ethanol; D000068180:Aripiprazole