title: Micro-computed tomography analysis of early stage bone healing using micro-porous titanium mesh for guided bone regeneration: preliminary experiment in a canine model.
abstract: The aim of this study was to evaluate the amount of bone formation beneath a defect area after treatment with titanium mesh membranes with different thicknesses and pore sizes alone or in combination with bone graft to induce bone formation during the early stage of healing time. The mandibular premolars were extracted bilaterally from three adult beagle dogs, and 8-mm-diameter bone defects were created on the buccal site of the premolar regions. Hydroxyapatite bone graft substitute was applied in the defect site unilaterally, and other site was left empty. Then, a novel micro-porous mesh (50 μm in pore diameter) or commercially available macro-porous titanium mesh (1700 μm in pore diameter) was placed on the defect and secured with screws. After 4 weeks, the mandibles were harvested, imaged using micro-computed tomography, and prepared for histological and morphometric evaluation. Higher new bone volumes (mm3), percentage of new bone volumes in the total defect volumes (bone ratio: %), and new bone area (mm2) through morphometric evaluation were found on the novel membranes with 50-μm-diameter pores compared to the commercial titanium mesh. Moreover, experiment sites without bone graft were observed with higher new bone volume and bone ratio compared with sites with bone graft. However, bone mineral density of novel mesh was observed to be lower compared with other experimental sites. Under the experimental condition, the result of this study suggests that titanium meshes with 50-μm-diameter pores were effective for guided bone regeneration in the early stage of healing.
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. rakhmatia@dent.kyushu-u.ac.jp.
authors.forename: Yunia Dwi
authors.initials: YD
authors.lastname: Rakhmatia
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ayukawa
authors.affiliation: Department of Oral and Maxillofacial Surgery and Oral Medicine, Faculty of Odontology, Malmö University, Carl Gustafs väg 34, SE 205 06, Malmö, Sweden.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Jinno
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Furuhashi
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Koyano
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology; D004285:Dogs; D017886:Durapatite / Q000494:pharmacology; D048091:Guided Tissue Regeneration / Q000379:methods*; D008334:Mandible / Q000601:surgery*; D008422:Materials Testing; D008567:Membranes, Artificial; D023421:Models, Animal; D016062:Porosity; D019736:Prostheses and Implants; D013499:Surface Properties; D013526:Surgical Mesh; D014025:Titanium; D014945:Wound Healing / Q000502:physiology*; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D008567:Membranes, Artificial; D017886:Durapatite; D014025:Titanium
keywords: Dog; Guided bone regeneration; Mandible defect; Micro-CT; Titanium mesh