title: A new prenylated flavone from Pleione bulbocodioides.
abstract: A new prenylated flavone, 3,5,7,3'-tetrahydroxy-8,4'-dimethoxy-6-(3-methylbut-2- enyl)flavone (1), together with three known flavone derivatives (2-4), two known dihydrophenanthrenes (5-6), two known lignin derivatives (7-8), and three known phenolic glycoside compounds (9-11) were isolated from the n-BuOH extract of Pleione bulbocodioides. High-resolution electrospray ionization mass spectroscopy (HRESIMS), FTIR spectroscopy, and NMR techniques were used to elucidate the structures of these compounds. Biological investigations showed that compound 5, 4,7-dihydroxy-2-methoxy-9,10-dihydrophenanthrene, exhibited potent anti-inflammatory activity on LPS-stimulated NO production in BV-2 microglial cells, with IC50value of 5.44 μM.
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: b The Eighth Affiliated Hospital , Sun Yat-Sen University , Shenzhen 518033 , China.|c Integrated Chinese and Western Medicine Postdoctoral Research Station , Jinan University , Guangzhou 510632 , China.
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Wu
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Ke-Wu
authors.initials: KW
authors.lastname: Zeng
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Ming-Bo
authors.initials: MB
authors.lastname: Zhao
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D029595:Orchidaceae / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry; D054876:Prenylation; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C000621751:3,5,7,3'-tetrahydroxy-8,4'-dimethoxy-6-(3-methylbut-2-enyl)flavone; D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D047309:Flavones; D008070:Lipopolysaccharides; D010616:Phenanthrenes; C505281:lusianthridin; D009569:Nitric Oxide
keywords: Orchidaceae; Pleione bulbocodioides; anti-inflammatory activity; prenylated flavone