title: Fluorescence properties of doxorubicin in PBS buffer and PVA films.
abstract: We studied steady-state and time-resolved fluorescence properties of an anticancer drug Doxorubicin in a saline buffer and poly-vinyl alcohol (PVA) film. Absorption of Doxorubicin, located at blue-green spectral region, allows a convenient excitation with visible light emitting diodes or laser diodes. Emission of Doxorubicin with maximum near 600nm can be easily detected with photomultipliers and CCD cameras. Both, absorption and fluorescence spectra in polymeric matrix show more pronounced vibronic structures than in solution. Also, the steady-state anisotropy in the polymer film is significantly higher than in the saline solution. In PVA film the fluorescence anisotropy is about 0.30 whereas in the saline buffer only 0.07. Quantum efficiencies of Doxorubicin were compared to a known standard Rhodamine 101 which has fluorescence emission in a similar spectral region. The quantum yield of Doxorubicin in PVA film is more than 10% and about twice higher than in the saline solution. Similarly, the lifetime of doxorubicin in PVA film is about 2ns whereas in the saline solution only about 1ns. The fluorescence anisotropy decays show that Doxorubicin molecules are freely rotating in the saline buffer with a correlation time of about 290ps, and are almost completely immobilized in the PVA film. The spectroscopic investigations presented in this manuscript are important, as they provide answers to changes in molecular properties of Doxorubicin depending changes in the local environment, which is useful when synthesizing nanoparticles for Doxorubicin entrapment.
authors.affiliation: Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. Electronic address: ss1069@live.unthsc.edu.
authors.forename: Sunil
authors.initials: S
authors.lastname: Shah
authors.affiliation: Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
authors.forename: Anjali
authors.initials: A
authors.lastname: Chandra
authors.affiliation: School of Public Health, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
authors.forename: Amanjot
authors.initials: A
authors.lastname: Kaur
authors.affiliation: Department of Integrative Physiology and Anatomy, UNT Health Science Center, Fort Worth, TX, USA.
authors.forename: Nirupama
authors.initials: N
authors.lastname: Sabnis
authors.affiliation: Department of Integrative Physiology and Anatomy, UNT Health Science Center, Fort Worth, TX, USA.
authors.forename: Andras
authors.initials: A
authors.lastname: Lacko
authors.affiliation: Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA; Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.affiliation: Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
authors.forename: Rafal
authors.initials: R
authors.lastname: Fudala
authors.affiliation: Institute for Molecular Medicine, Program in Fluorescence Technologies at Center for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski
mesh_terms: D002021:Buffers; D004317:Doxorubicin / Q000737:chemistry*; D005454:Fluorescence Polarization; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011789:Quantum Theory; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D002021:Buffers; D004317:Doxorubicin; D011142:Polyvinyl Alcohol