title: Efficacy of curcumin in inducing apoptosis and inhibiting the expression of VEGF in human pterygium fibroblasts.
abstract: Pterygium is an inflammatory and degenerative ocular surface disease, in which the conjunctiva on the cornea grows to form a fibrous tissue in the shape of a triangle. Although pterygium surgery is used broadly in clinical practice, its high recurrence rate poses a great concern for patients and doctors. In the present study, we examined the effects of curcumin on the apoptosis and proliferation of human pterygium fibroblasts (HPFs) in an in vitro culture system. HPFs were incubated with curcumin for 24 to 72 h (5, 20, 80 or 200 µmol/l). The proliferation-inhibiting effects of curcumin were assayed by MTT assay at different time points and at various concentrations. The expression of vascular endothelial growth factor (VEGF) in each group was detected by RT-qPCR and ELISA. The apoptotic effects of curcumin were detected by flow cytometry (FCM). Treatment with curcumin at 80 µmol/l for 48 h significantly inhibited HPF proliferation. Curcumin significant induced the apoptosis of HPFs. Our findings thus suggest that curcumin suppress cell proliferation in the pterygium by inducing HPF apoptosis and inhibiting VEGF expression. Thus, curcumin has potential for use as a novel agent for the treatment of pterygium in the near future.
authors.affiliation: Department of Ophthalmology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Lu
authors.affiliation: Department of Ophthalmology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Hao
authors.affiliation: Department of Dermatology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yao
authors.affiliation: Translational Medicine Research Institute, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Li
authors.affiliation: Department of Radiology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011625:Pterygium / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D003474:Curcumin