title: Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model.
abstract: Red seaweed (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) were digested using in vitro mouth, gastric and duodenal model. The digested seaweed then fermented in a pH-controlled batch culture system inoculated with human faeces to mimic the distal colon. Bacterial enumeration were monitored using fluorescent in situ hybridisation, and the fermentation end products, the short chain fatty acids (SCFA), were analysed using HPLC. Both RSS and RSL showed significant increase of Bifidobacterium sp.; from log107.96 at 0 h to log108.72 at 24 h, and from log107.96 at 0 h to log108.60 at 24 h, respectively, and shows no significant difference when compared to the Bifidobacterium sp. count at 24 h of inulin fermentation. Both seaweeds also showed significant increase in total SCFA production, particularly acetate and propionate. Overall, this data suggested that K. alvarezii might have the potential as a prebiotic ingredient.
authors.affiliation: a Department of Crop Science, Faculty of Agricultural and Food Sciences , Universiti Putra , Malaysia Bintulu Campus , Sarawak , Malaysia.
authors.forename: Dayang Marshitah
authors.initials: DM
authors.lastname: Bajury
authors.affiliation: a Department of Crop Science, Faculty of Agricultural and Food Sciences , Universiti Putra , Malaysia Bintulu Campus , Sarawak , Malaysia.
authors.forename: Muhamad Hanif
authors.initials: MH
authors.lastname: Rawi
authors.affiliation: a Department of Crop Science, Faculty of Agricultural and Food Sciences , Universiti Putra , Malaysia Bintulu Campus , Sarawak , Malaysia.
authors.forename: Iqbal Hakim
authors.initials: IH
authors.lastname: Sazali
authors.affiliation: b School of Chemical Sciences and Food Technology, Faculty of Science and Technology , Universiti Kebangsaan Malaysia , Bangi , Selangor , Malaysia.
authors.forename: Aminah
authors.initials: A
authors.lastname: Abdullah
authors.affiliation: a Department of Crop Science, Faculty of Agricultural and Food Sciences , Universiti Putra , Malaysia Bintulu Campus , Sarawak , Malaysia.
authors.forename: Shahrul Razid
authors.initials: SR
authors.lastname: Sarbini
mesh_terms: D001644:Bifidobacterium; D003106:Colon / Q000502:physiology*; D005285:Fermentation; D006801:Humans; D007444:Inulin; D008954:Models, Biological; D056692:Prebiotics; D000461:Rhodophyta; D012624:Seaweed
chemical_list: D056692:Prebiotics; D007444:Inulin
keywords: Bifidobacteria; Prebiotic; colon model; gut microbiota; seaweed