title: Differential colonization by bioprospected rhizobial bacteria associated with common bean in different cropping systems.
abstract: In this study, we evaluated the diversity of rhizobia isolated from root nodules on common bean (Phaseolus vulgaris) derived from Andean and Mesoamerican centers and grown under field and greenhouse conditions. Genetic characterization of isolates was performed by sequencing analyses of the 16S rRNA gene and 2 housekeeping genes, recA and glnII, and by the amplification of nifH. Symbiotic efficiency was evaluated by examining nodulation, plant biomass production, and plant nitrogen (N) accumulation. The influence of the environment was observed in nodulation capacity, where Rhizobium miluonense was dominant under greenhouse conditions and the Rhizobium acidisoli group prevailed under field conditions. However, strain LGMB41 fit into a separate group from the type strain of R. acidisoli in terms of multilocus phylogeny, implying that it could belong to a new species. Rhizobium miluonense LGMB73 showed the best symbiotic efficiency performance, i.e., with the highest shoot-N content (77.7 mg/plant), superior to the commercial standard strain (56.9 mg/plant). Biodiversity- and bioprospecting-associated studies are important to better understand ecosystems and to develop more effective strategies to improve plant growth using a N-fixation process.
authors.affiliation: a Universidade Federal do Paraná, Department of Genetics, C.P. 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: Josiele Polzin
authors.initials: JP
authors.lastname: de Oliveira-Francesquini
authors.affiliation: b Embrapa Soja, C.P. 231, 86001-970, Londrina, PR, Brazil.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Hungria
authors.affiliation: a Universidade Federal do Paraná, Department of Genetics, C.P. 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: Daiani Cristina
authors.initials: DC
authors.lastname: Savi
authors.affiliation: a Universidade Federal do Paraná, Department of Genetics, C.P. 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: Chirlei
authors.initials: C
authors.lastname: Glienke
authors.affiliation: a Universidade Federal do Paraná, Department of Genetics, C.P. 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Aluizio
authors.affiliation: a Universidade Federal do Paraná, Department of Genetics, C.P. 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Kava
authors.affiliation: a Universidade Federal do Paraná, Department of Genetics, C.P. 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: Lygia Vitoria
authors.initials: LV
authors.lastname: Galli-Terasawa
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics / Q000302:isolation & purification*; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Phaseolus vulgaris; Rhizobium; analyse multilocus; biological nitrogen fixation; cropping system; fixation biologique d’azote; multilocus analysis; système de culture