title: Non-photonic sensing of membrane-delimited reactive species with a Na+channel protein containing selenocysteine.
abstract: Photonic experiments are of key importance in life sciences but light-induced side effects are serious confounding factors. Here we introduce roNaV2, an engineered voltage-gated Na+channel harboring a selenocysteine in its inactivation motif, as a non-photonic, sensitive, gateable, and reversible sensor for membrane-delimited reactive species. roNaV2 allows for the assessment of chemical modification induced in fluorescence microscopy settings with high sensitivity and time resolution and it demonstrates the usefulness of ion channels as highly sensitive reporters of membrane processes.
authors.affiliation: Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena &Jena University Hospital, Jena, Germany.
authors.forename: Navin K
authors.initials: NK
authors.lastname: Ojha
authors.affiliation: Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena &Jena University Hospital, Jena, Germany.
authors.forename: Enrico
authors.initials: E
authors.lastname: Leipold
authors.affiliation: Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena &Jena University Hospital, Jena, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Schönherr
authors.affiliation: Department of Physiology, University of Pennsylvania, Philadelphia, USA.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Hoshi
authors.affiliation: Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena &Jena University Hospital, Jena, Germany.
authors.forename: Stefan H
authors.initials: SH
authors.lastname: Heinemann
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D010084:Oxidation-Reduction; D017785:Photons; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017279:Selenocysteine / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism*; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D015222:Sodium Channels; D017279:Selenocysteine