title: DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater.
abstract: PCR inhibitory substances in complex sample matrices can cause false negatives or under-estimation of target concentration. This study assessed DNA heat treatment for reducing inhibition during qPCR analysis of human adenovirus (HAdV) in wastewater samples. Inhibition was reduced by heat treating DNA, where mean HAdV concentration was increased by 0.71 log10GC/L (and up to 3.04 log10GC/L in one case), and replicate variability and false negatives were reduced. DNA heat treatment should be further investigated for improving reliability of HAdV concentration estimates in water, which can support more accurate assessment of health risks associated with viral pathogen exposure.
authors.affiliation: Genecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, QLD, 4556, Australia.
authors.forename: Emily
authors.initials: E
authors.lastname: Rames
authors.affiliation: School of Medicine and Menzies Health Institute Queensland, Griffith University, Nathan, Australia.
authors.forename: Anne
authors.initials: A
authors.lastname: Roiko
authors.affiliation: School of Natural Sciences, Griffith University, Nathan, Australia.
authors.forename: Helen
authors.initials: H
authors.lastname: Stratton
authors.affiliation: Genecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, QLD, 4556, Australia. jmacdon1@usc.edu.au.|Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, USA. jmacdon1@usc.edu.au.
authors.forename: Joanne
authors.initials: J
authors.lastname: Macdonald
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D062065:Wastewater / Q000821:virology*
chemical_list: D004279:DNA, Viral; D062065:Wastewater
keywords: Adenovirus; DNA heat treatment; Health risk; Inhibitors; QPCR; Wastewater