title: Study of the Binding between Camptothecin Analogs and FTO by Spectroscopy and Molecular Docking.
abstract: In this work, the interaction between camptothecin (CPT) analogs and fat mass and obesity associated (FTO) was investigated using spectroscopy and molecular docking. From the experimental results, it was found that the CPT analogs caused the fluorescence quenching of FTO through a static quenching procedure. The binding constants and thermodynamic parameters at three different temperatures, the number of binding sites were obtained, which suggested that the hydrophobic interaction and electrostatic force played major role in the reaction between CPT analogs and FTO. Results revealed that 10-hydroxycamptothecin was the strongest quencher.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ren
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China.
authors.forename: Zechun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China.
authors.forename: Lijiao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Han
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China. wangry@zzu.edu.cn.
authors.forename: Ruiyong
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China. changjunbiao@zzu.edu.cn.
authors.forename: Junbiao
authors.initials: J
authors.lastname: Chang
keywords: Binding; Camptothecin analogs; FTO; Fluorescence; Molecular modeling