title: DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 2 - Ocular DynaMiTES for drug absorption studies of the anterior eye.
abstract: In the present study, a formerly designed Dynamic Micro Tissue Engineering System (DynaMiTES) was applied with our prevalidated human hemicornea (HC) construct to obtain a test platform for improved absorption studies of the anterior eye (Ocular DynaMiTES). First, the cultivation procedure of the classic HC was slightly adapted to the novel DynaMiTES design. The obtained inverted HC was then compared to classic HC regarding cell morphology using light and scanning electron microscopy, cell viability using MTT dye reaction and epithelial barrier properties observing transepithelial electrical resistance and apparent permeation coefficient of sodium fluorescein. These tested cell criteria were similar. In addition, the effects of four different flow rates on the same cell characteristics were investigated using the DynaMiTES. Because no harmful potential of flow was found, dynamic absorption studies of sodium fluorescein with and without 0.005%, 0.01% and 0.02% benzalkonium chloride were performed compared to the common static test procedure. In this proof-of-concept study, the dynamic test conditions showed different results than the static test conditions with a better prediction of in vivo data. Thus, we propose that our DynaMiTES platform provides great opportunities for the improvement of common in vitro drug testing procedures.
authors.affiliation: Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Mendelssohnstraβe 1, 38106 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Beiβner
authors.affiliation: Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Straβe 203, 38124 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Mattern
authors.affiliation: Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Straβe 203, 38124 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Dietzel
authors.affiliation: Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Mendelssohnstraβe 1, 38106 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany. Electronic address: s.reichl@tu-braunschweig.de.
authors.forename: Stephan
authors.initials: S
authors.lastname: Reichl
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003315:Cornea / Q000187:drug effects / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004867:Equipment Design / Q000379:methods; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D023822:Tissue Engineering / Q000379:methods*; D057705:Transendothelial and Transepithelial Migration / Q000187:drug effects / Q000502:physiology
chemical_list: D005456:Fluorescent Dyes
keywords: Benzalkonium chloride; DynaMiTES; Dynamic flow system; Human hemicornea construct; In vitro drug absorption; Microfluidic system; Organ on chip; TEER measurement