title: Viral and serological kinetics in Zika virus-infected patients in South Korea.
abstract: Zika virus is a mosquito-borne flavivirus that causes clinical symptoms similar to those observed in dengue and chikungunya virus infections. The Korea Centers for Disease Control and Prevention initiated laboratory testing using a real-time reverse transcription-polymerase chain reaction in January 2016. More than 1,000 suspected cases of infection were tested and nine were confirmed as imported cases of Zika virus infection from January to July 2016. The travel destinations of the infected individuals were Brazil, Philippines, Viet Nam, Guatemala, Puerto Rico, and the Dominican Republic. Phylogenetic analysis based on the partial envelope gene indicated that the viruses belonged to the Asian genotype circulating in South America. We further investigated the duration for which the viral RNA and virus-specific antibodies were detectable after the symptom onset. After the day of symptom onset, Zika virus was detectable until 6 days in serum, 14 days in urine and saliva, and 58 days in semen. Immunoglobulin M against Zika virus was detected as early as 2 days after the symptom onset and was maintained at these levels until 41 days, whereas Immunoglobulin G was detectable from 8 days after the symptom onset and was maintained until 52 days. These findings would help diagnostic laboratories improve their testing programs for Zika virus infection.
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-yeup, Cheongju-si, Chungbuk-do, 28159, South Korea.
authors.forename: Young Eui
authors.initials: YE
authors.lastname: Jeong
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-yeup, Cheongju-si, Chungbuk-do, 28159, South Korea.
authors.forename: Go-Woon
authors.initials: GW
authors.lastname: Cha
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-yeup, Cheongju-si, Chungbuk-do, 28159, South Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Cho
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-yeup, Cheongju-si, Chungbuk-do, 28159, South Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Korea Centers for Disease Control and Prevention, Cheongju-si, 28159, Chungbuk-do, South Korea.
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jee
authors.affiliation: Division of Arboviruses, National Institute of Health, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-yeup, Cheongju-si, Chungbuk-do, 28159, South Korea. leewonja@gmail.com.
authors.forename: Won-Ja
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D001826:Body Fluids / Q000821:virology; D005838:Genotype; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010802:Phylogeny; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000235:genetics; D019562:Viral Load; D000071244:Zika Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000276:immunology* / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014759:Viral Envelope Proteins
keywords: Chikungunya virus; Dengue virus; Enzyme-Linked Immunosorbent Assay; Phylogenetic analysis; Reverse transcription-polymerase chain reaction; Zika virus