title: [PD-1/PD-L1 expression and its implications in patients with chronic lymphocytic leukemia].
abstract: Objective:To observe the expression levels of PD-1/PD-L1 costimulatory molecules and explore the clinical significance in patients with chronic lymphocytic leukemia (CLL) .Methods:The expression of PD-1/PD-L1 in peripheral blood CD8(+) T cells, CD4(+)T cells, CD19(+)B, and dendrites cells (DC) was detected by flow cytometry in 57 CLL patients and 20 healthy controls. The correlations of PD-1/PD-L1 expression with disease stage, CD38 expression, ZAP-70 expression, chromosome karyotype abnormality and β(2)-MG expression were analyzed.Results:①Compared with control, CLL patients, including 39 males and 18 females with the median age of (63.7±10.7) years, had no statistically significant difference in age and gender (P>0.05) . CLL patients had the higher PD-1/PD-L1 expression than healthy controls (P<0.05) . ②In Rai staging, the later the stage, the higher expression of PD-1/PD-L1 (P<0.05) . ③PD-1 expression in CD8(+)CD38(+) group (11 cases) was higher than that in CD8(+)CD38(-) group (46 cases) (P=0.004) , and CD8(+) poor prognosis chromosome group (14 cases) also had significant higher PD-1 expression than CD8(+)good prognosis chromosome group (28 cases) (P=0.004) . ④The expression of PD-L1 was higher in CD38(+)group, ZAP-70(+)group, and poor prognosis group, as compared to that in CD38(-)group (P=0.002) , in ZAP-70(-)group (P<0.001) , in good prognosis group (P=0.023) . There was no correlation between the expression of PD-1/PD-L1 and β(2)-MG (P>0.05) .Conclusion:This data reveals that PD-1/PD-L1 was highly expressed in CLL patients. Its expression levels were correlated with Rai stage, CD38, ZAP-70, chromosome karyotype, but not with β(2)-MG. PD-1/PD-L1 may be a prognostic factor in patients with CLL.
authors.affiliation: Xinjiang Medical University First Affiliated Hospital Blood Disease Center, Xinjiang Uygur Autonomous Region of Hematology, Urumqi 830054, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Li
authors.forename: N N
authors.initials: NN
authors.lastname: Pang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: J H
authors.initials: JH
authors.lastname: Qu
mesh_terms: D051997:ADP-ribosyl Cyclase 1; D000368:Aged; D060890:B7-H1 Antigen; D018414:CD8-Positive T-Lymphocytes; D002869:Chromosome Aberrations; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human; D051997:ADP-ribosyl Cyclase 1
keywords: Antigens, CD274; Leukemia, lymphocytic, chronic, B-cell; Programmed cell death 1 receptor