title: Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn.
abstract: Hyperpigmentation in Silky Fowl (SF) results in aberrant immune cell development. However, how melanocytes regulate B-cell proliferation in the bursa of Fabricius (BF) is unclear. To resolve this conundrum, we collected BFs from three-week-old SF and White Leghorn (WL) female chickens for RNA sequencing. The BF development was relatively weaker in SF than in WL. The transcriptome analyses identified 4848 differentially expressed genes, 326 long noncoding RNAs (lncRNAs), and 67 microRNAs in the BF of SF. The genes associated with melanogenesis was significantly higher, but that of the genes associated with the cytokine-cytokine receptor interactions and JAK-STAT signalling pathway was significantly lower in SF than in WL. Crucial biological processes, such as the receptor activity, cell communication, and cellular responses to stimuli, were clustered in SF. The predicted target lncRNAs genes were mainly associated with cell proliferation pathways such as JAK-STAT, WNT, MAPK, and Notch signalling pathways. Except for the above pathways, the target microRNA genes were related to the metabolism, melanogenesis, autophagy, and NOD-like and Toll-like receptor signalling pathways. The lncRNAs and microRNAs were predicted to regulate the JAK2, STAT3, and IL-15 genes. Thus, B-cell development in the BF of SF might be regulated and affected by noncoding RNAs.
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.|College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Deping
authors.initials: D
authors.lastname: Han
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Hua
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Science, Northeastern University, Shenyang 110004, China.
authors.forename: Xuegong
authors.initials: X
authors.lastname: Deng
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000166:cytology / Q000378:metabolism*; D002645:Chickens / Q000235:genetics*; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding