title: Downregulation of lncRNA ANRIL inhibits proliferation, induces apoptosis, and enhances radiosensitivity in nasopharyngeal carcinoma cells through regulating miR-125a.
abstract: Increasing evidence demonstrated that long non-coding RNA ANRIL serves as a fatal oncogene in many cancers, including nasopharyngeal carcinoma (NPC). However, little is known whether ANRIL regulated NPC cell radioresistance. Quantitative real-time PCR (qRT-PCR) was performed to examine the expression of lncRNA ANRIL and miR-125a in NPC tissues and cell lines. MTT assay was conducted to measure the cell viability of CNE2 and HONE1 cells. The apoptotic rate of CNE2 and HONE1 cells was determined by flow cytometry analysis. Colony survival was determined by clonogenic assay. Luciferase reporter assay was performed to verity the direct target of miR-125a. LncRNA ANRIL was evidently elevated in NPC tissues and cell lines. ANRIL inhibition suppressed proliferation, induced apoptosis, and enhanced radiosensitivity in NPC. Moreover, ANRIL could negatively modulate miR-125a expression. Furethermore, ANRIL upregulation reserved the inhibited proliferation, induced apoptosis, and enhanced radiosensitivity triggered by miR-125a overexpression. The expression of lncRNA ANRIL was upregulated in NPC tissues and cells. Moreover, knockdown of ANRIL repressed proliferation, promoted apoptosis, and improved radiosensitivity in NPC via functioning as a miR-125a sponge.
authors.affiliation: a Department of Radiotherapy , Huaihe Hospital of Henan University , Kaifeng , China.
authors.forename: Xigang
authors.initials: X
authors.lastname: Hu
authors.affiliation: a Department of Radiotherapy , Huaihe Hospital of Henan University , Kaifeng , China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: b Department of Oncology , The Second Division Korla Hospital of Xinjiang Production and Construction Corps , Korla , China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics / Q000528:radiation effects; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D062085:RNA, Long Noncoding / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics
chemical_list: C561518:CDKN2B antisense RNA, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: ANRIL; NPC; apoptosis; miR-125a; proliferation; radiosensitivity