title: Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress.
abstract: The aim of this study was to examine the effect of molecular hydrogen (H2) on the healing of alkali-injured cornea. The effects of the solution of H2in phosphate buffered saline (PBS) or PBS alone topically applied on the alkali-injured rabbit cornea with 0.25 M NaOH were investigated using immunohistochemical and biochemical methods. Central corneal thickness taken as an index of corneal hydration was measured with an ultrasonic pachymeter. Results show that irrigation of the damaged eyes with H2solution immediately after the injury and then within next five days renewed corneal transparency lost after the injury and reduced corneal hydration increased after the injury to physiological levels within ten days after the injury. In contrast, in injured corneas treated with PBS, the transparency of damaged corneas remained lost and corneal hydration elevated. Later results-on day 20 after the injury-showed that in alkali-injured corneas treated with H2solution the expression of proinflammatory cytokines, peroxynitrite, detected by nitrotyrosine residues (NT), and malondialdehyde (MDA) expressions were very low or absent compared to PBS treated injured corneas, where NT and MDA expressions were present. In conclusion, H2solution favorably influenced corneal healing after alkali injury via suppression of oxidative stress.
authors.affiliation: Institute of Experimental Medicine, Czech Academy of Sciences, 14220 Prague 4, Czech Republic.
authors.forename: Cestmir
authors.initials: C
authors.lastname: Cejka
authors.affiliation: Institute of Experimental Medicine, Czech Academy of Sciences, 14220 Prague 4, Czech Republic; Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kossl
authors.affiliation: Institute of Experimental Medicine, Czech Academy of Sciences, 14220 Prague 4, Czech Republic; Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Hermankova
authors.affiliation: Institute of Experimental Medicine, Czech Academy of Sciences, 14220 Prague 4, Czech Republic; Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Holan
authors.affiliation: Institute of Experimental Medicine, Czech Academy of Sciences, 14220 Prague 4, Czech Republic.
authors.forename: Jitka
authors.identifier: 0000-0001-7578-2084
authors.initials: J
authors.lastname: Cejkova
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D003315:Cornea / Q000378:metabolism / Q000473:pathology; D065306:Corneal Injuries / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006859:Hydrogen / Q000494:pharmacology*; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D053551:Keratin-12 / Q000378:metabolism; D053558:Keratin-3 / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D030421:Peroxynitrous Acid / Q000378:metabolism; D011817:Rabbits; D012972:Sodium Hydroxide / Q000633:toxicity*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D016207:Cytokines; D053583:Interleukin-1beta; D053551:Keratin-12; D053558:Keratin-3; D042461:Vascular Endothelial Growth Factor A; D030421:Peroxynitrous Acid; D008315:Malondialdehyde; D012972:Sodium Hydroxide; D006859:Hydrogen