title: Direct effects of interleukin-7 on the function of human T cells in vitro.
abstract: СD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in СD4-positive and in CD4-negative effector memory (CD45RA-CD197-) T cell subsets, as well as in terminally differentiated (CD45RA+CD197-) Т cells, without significantly affecting the activation status of naive (CD45RA+CD197+) and central memory (CD45RA-CD197+) T cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4, in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBead™ particles coated with CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA-CD197+), and effector memory (CD45RA-CD197-) T-cell compartments. In addition, IL-7 facilitated activation of СD4-(but not CD4+) terminally differentiated effector (CD45RA+CD197-) Т cells. Finally, IL-7 was found to upregulate the production of IL-2, IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional T cell activity without causing significant functional inbalance between various T cell subsets.
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Vyacheslav A
authors.initials: VA
authors.lastname: Shmarov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Malashchenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Maksim E
authors.initials: ME
authors.lastname: Meniailo
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Gazatova
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Todosenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Olga B
authors.initials: OB
authors.lastname: Melashchenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Andrei G
authors.initials: AG
authors.lastname: Goncharov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Victor I
authors.initials: VI
authors.lastname: Seledtsov
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000187:drug effects*; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D015851:Interleukin-7 / Q000276:immunology / Q000494:pharmacology*; D008297:Male
chemical_list: C573415:IFNG protein, human; C508609:IL10 protein, human; C508594:IL2 protein, human; C508598:IL4 protein, human; C508605:IL7 protein, human; D007376:Interleukin-2; D015851:Interleukin-7; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: CD25; T cell; T-cell activation; cytokine production; homeostatic activity; interleukin-7