title: Immobilization on Metal-Organic Framework Engenders High Sensitivity for Enzymatic Electrochemical Detection.
abstract: The protection effect of metal-organic framework (MOF) provides high stability for immobilized enzyme. The small cavities of MOFs, however, usually result in decreased apparent substrate affinity and enzymatic activity of immobilized enzyme, compared to native enzyme. We synthesized zeolitic imidazolate framework-8 (ZIF-8) with a combination of mesoporous and microporous channels for cytochrome c (Cyt c) immobilization. Compared with native Cyt c, the immobilized Cyt c displayed increased apparent substrate affinity (Michaelis constant Kmreduced by ∼50%), ∼128% increased enzymatic activity, and 1.4-fold increased sensitivity in the enzymatic electrochemical detection of H2O2. The immobilized Cyt c-coated screen-printed electrode was applied for the fast detection of residual H2O2in microliter food samples such as milk and beer, making it promising for the development of efficient biosensors.
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Xuerui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Hou
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Jun
authors.identifier: http://orcid.org/0000-0001-5503-8899
authors.initials: J
authors.lastname: Ge
mesh_terms: D015374:Biosensing Techniques; D045304:Cytochromes c; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D000073396:Metal-Organic Frameworks / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D000073396:Metal-Organic Frameworks; D045304:Cytochromes c; D006861:Hydrogen Peroxide
keywords: biosensor; cytochrome c; electrochemical detection; enzyme immobilization; metal-organic frameworks