title: Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle.
abstract: Endometrial receptivity is crucial for implantation and establishment of a normal pregnancy. The shift from proliferative to receptive endometrium is still far from being understood. In this paper, we comprehensively present the transcriptome of the human endometrium by comparing endometrial biopsies from proliferative phase with consecutive biopsies 7-9 days after ovulation. The results show a clear difference in expression between the two time points using both total and small RNA sequencing. A total of 3,297 messenger RNAs (mRNAs), 516 long noncoding RNAs (lncRNAs), and 102 small noncoding RNAs were identified as statistically differentially expressed between the two time points. We show a thorough description of the change in mRNA between the two time points and display lncRNAs, small nucleolar RNAs, and small nuclear RNAs not previously reported in the healthy human endometrium. In conclusion, this paper reports in detail the shift in RNA expression from the proliferative to receptive endometrium.
authors.affiliation: Science for Life Laboratory, School of Biotechnology, Kungliga Tekniska högskolan Royal Institute of Technology, Solna, Sweden.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sigurgeirsson
authors.affiliation: Department of Clinical Science and Education, Unit of Obstetrics and Gynecology, Karolinska Institute, Södersjukhuset, Stockholm, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Åmark
authors.affiliation: Science for Life Laboratory, School of Biotechnology, Kungliga Tekniska högskolan Royal Institute of Technology, Solna, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Jemt
authors.affiliation: Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden.
authors.forename: Dorina
authors.initials: D
authors.lastname: Ujvari
authors.affiliation: Department of Clinical Sciences, Intervention and Technology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Westgren
authors.affiliation: Science for Life Laboratory, School of Biotechnology, Kungliga Tekniska högskolan Royal Institute of Technology, Solna, Sweden.
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.affiliation: Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden.|Department of Clinical Sciences, Intervention and Technology, Karolinska Institute, Stockholm, Sweden.|Department of Obstetrics and Gynecology, Karolinska University Hospital Huddinge, Stockholm, Sweden.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gidlöf
mesh_terms: D000328:Adult; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase / Q000378:metabolism*; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism*; D061251:Primary Cell Culture; D025341:Principal Component Analysis; D012313:RNA / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D055815:Young Adult
chemical_list: D012313:RNA
keywords: RNA sequencing; endometrium; receptivity