title: A Proteomic Analysis of the Malignant Mesothelioma Secretome Using iTRAQ.
abstract: Backgound/Aim: Malignant mesothelioma (MM) is an aggressive and fatal pleural cancer. The cell secretome offers information allowing insight into the pathogenesis of MM while offering the possibility to identify potential therapeutic targets and biomarkers. In the present study the secretome protein profile of MM cell lines was compared to normal mesothelial cells.
  
  
  
  Six MM cell lines were compared against three primary mesothelial cell culture preparations using iTRAQ® mass spectrometry.
  
  
  
  MM cell lines more abundantly secreted exosome-associated proteins than mesothelial cells. MM cell secretomes were enriched in proteins that are involved in response to stress, carbon metabolism, biosynthesis of amino acids, antigen processing and presentation and protein processing in the endoplasmic reticulum.
  
  
  
  The MM cell secretome is enriched in proteins that are likely to enhance its growth and response to stress and help it inhibit an adaptive immune response. These are potential targets for therapeutic and biomarker discovery.
authors.affiliation: National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia and Australian Mesothelioma Tissue Bank, Sir Charles Gairdner Hospital, Perth, Western Australia jenette.creaney@uwa.edu.au.
authors.forename: Jenette
authors.initials: J
authors.lastname: Creaney
authors.affiliation: National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Sir Charles Gairdner Hospital, Perth, Western Australia.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Dick
authors.affiliation: National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Sir Charles Gairdner Hospital, Perth, Western Australia.
authors.forename: Justine S
authors.initials: JS
authors.lastname: Leon
authors.affiliation: National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Sir Charles Gairdner Hospital, Perth, Western Australia.
authors.forename: Bruce W S
authors.initials: BW
authors.lastname: Robinson
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D016000:Cluster Analysis; D055354:Exosomes / Q000235:genetics / Q000378:metabolism; D005260:Female; D063990:Gene Ontology; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008654:Mesothelioma / Q000378:metabolism / Q000473:pathology; D000086002:Mesothelioma, Malignant; D008875:Middle Aged; D025341:Principal Component Analysis; D020543:Proteome / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: Malignant mesothelioma; iTRAQ; proteomics; secretome