title: Interleukin-10 and Interferon Gamma Gene Polymorphisms and Hepatitis C Virus-Related Liver Cirrhosis Risk.
abstract: The aim of the study was to evaluate the association between the gene polymorphisms in interleukin-10 (IL-10) and interferon gamma (IFN-γ) genes with susceptibility and severity of hepatitis C virus (HCV) infection among Egyptian patients. Interleukin-10 -592 A/C, -1082 G/A and IFN-γ +874 T/A genotypes were determined in 100 chronic HCV patients and 50 healthy controls using restriction fragment length polymorphism (RFLP) and the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) respectively. IL-10 -592 A/C polymorphism genotyping revealed that the frequency of CC genotype was significantly higher in chronic HCV patients than in controls (58% versus 30%, P < 0.05). Regarding IL-10 -1082 G/A polymorphism genotyping, a higher frequency of GG genotype was found in chronic HCV patients compared to controls (31% versus 10%, P < 0.05). IFN-γ +874 T/A genotyping showed that TT genotype was significantly higher in chronic HCV participants than controls (31% versus 18%, P < 0.05), while a higher frequency of T allele was found in cirrhotic patients compared to noncirrhotic patients (P < 0.05). Our observations suggested that IL-10 -592 A/C, -1082 G/A, and IFN-γ +874 T/A polymorphisms had a strong association with susceptibility to HCV infection. However, no significant association was observed between the cytokines (IL-10 and IFN-γ) genotypes profile and HCV-liver cirrhosis risk in the studied population, except for the high frequency of IFN-γ +874 T allele in cirrhotic patients.
authors.affiliation: 1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University , Sohag, Egypt .
authors.forename: Abeer
authors.initials: A
authors.lastname: Sheneef
authors.affiliation: 1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University , Sohag, Egypt .
authors.forename: Mamdouh M
authors.initials: MM
authors.lastname: Esmat
authors.affiliation: 2 Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Sohag University , Sohag, Egypt .
authors.forename: Asmaa N
authors.initials: AN
authors.lastname: Mohammad
authors.affiliation: 3 Department of Medical Biochemistry, Faculty of Medicine, Sohag University , Sohag, Egypt .
authors.forename: Aida A
authors.initials: AA
authors.lastname: Mahmoud
authors.affiliation: 4 Department of Physiology, Faculty of Medicine, Sohag University , Sohag, Egypt .
authors.forename: Hoda M
authors.initials: HM
authors.lastname: Moghazy
authors.affiliation: 5 Department of Internal Medicine, Faculty of Medicine, Sohag University , Sohag, Egypt .
authors.forename: Amal K
authors.initials: AK
authors.lastname: Noureldin
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016174:Hepacivirus; D006526:Hepatitis C / Q000150:complications* / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008103:Liver Cirrhosis / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D019562:Viral Load
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma
keywords: gene polymorphism; hepatitis C virus; interferon-γ; interleukin-10