title: Identification of ACTG2 functions as a promoter gene in hepatocellular carcinoma cells migration and tumor metastasis.
abstract: Metastatic hepatocellular carcinoma (HCC) remains a mostly incurable disease. The fact that the identity of the mechanisms that regulate metastasis in HCC is known hampers the development of anti-metastatic therapies. Currently, there is no effective treatment for HCC once it is progressed to metastatic stage. Therefore, further study to elucidate the molecular mechanism underlying the metastasis of HCC is urgently required for the improvement of HCC treatment. Here, we describes actin gamma smooth muscle 2 (ACTG2) over-express in HCC and demonstrates high-expression of ACTG2 as a promising therapeutic target in HCC metastasis. The use of shRNA to knock-down ACTG2 impaired cells migration and invasion in vitro. Moreover, silencing of ACTG2 causes almost complete inhibition of metastasis in vivo. In contrast, overexpression ACTG2 significantly enforces HCC cells migration and metastasis. Finally, ACTG2 boosts the metastatic potential of HCC cells in a Notch homolog 1 (Notch1) dependent manner. Collectively, our study reveals a critical role of ACTG2 in HCC tumor metastasis, and renders it a novel target for the treatment of HCC.
authors.affiliation: Department of Pharmacy, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, Nantong 226001, China.
authors.forename: Zhao Guo
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Mei Qin
authors.initials: MQ
authors.lastname: Shi
authors.affiliation: Department of Clinical Laboratory, Nantong Hospital of Traditional Chinese Medicine, Nantong, 226001, China.
authors.forename: Hai Zhong
authors.initials: HZ
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: Department of Pharmacy, Nanton Maternal & Child Health Hospital, Nantong 226001, China.
authors.forename: Ai Hua
authors.initials: AH
authors.lastname: Yang
authors.affiliation: Natong Institute for Drug Control, Nantong 226001, China.
authors.forename: Xing Sheng
authors.initials: XS
authors.lastname: Fu
authors.affiliation: Department of Pharmacy, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Shuiying
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Oncology, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China; Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: shenshuijies@outlook.com.
authors.forename: Shui Jie
authors.initials: SJ
authors.lastname: Shen
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China. Electronic address: liweidong0801@outlook.com.
authors.forename: Wei Dong
authors.initials: WD
authors.lastname: Li
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000517:prevention & control / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D023041:Xenograft Model Antitumor Assays
chemical_list: C579475:ACTG2 protein, human; D000199:Actins; C497761:NOTCH1 protein, human; D034741:RNA, Small Interfering; D051881:Receptor, Notch1
keywords: ACTG2; HCC; Metastasis; Notch1