title: β-CD-Functionalized Microdevice for Rapid Capture and Release of Bacteria.
abstract: Most procedures for detecting pathogens in liquid media require an initial concentration step. In this regard, carbohydrates have proven to be attractive affinity ligands for the solid-phase capture of bacteria that use lectins for adhesion to host cell membranes. However, the use of cyclodextrin-immobilized substrates to selectively trap bacteria has not been explored before. Here, using quartz-crystal microbalance with dissipation monitoring experiments, we demonstrate that functionalization of surfaces by β-cyclodextrin (β-CD) can not only allow for rapid and efficient capture of bacterial cells in liquid but also their facile elution with an aqueous solution of a selectively methylated β-CD derivative as a competitive molecule. This capture/elution strategy, which is based on host-guest interactions between membrane components of the bacterial cell and the CD cavities, is performed in physiological conditions and can be integrated in a microchip. Indeed, proof-of-concept studies showed the potential of β-CD-modified micropillar-integrated microfluidic devices for concentration of bacteria. The results obtained with Escherichia coli suggest that this approach could be broadly applicable among Gram-negative bacteria, which share common cell membrane structures.
authors.affiliation: Grenoble Alpes University and Centre de Recherches sur les Macromolécules Végétales , 601, rue de la Chimie, BP 53, 38041 Grenoble Cedex 9, France.|Grenoble Alpes University and CEA LETI MlNATEC Campus , 17, avenue des Martyrs, 38054 Grenoble, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Perez-Anes
authors.affiliation: Grenoble Alpes University and Centre de Recherches sur les Macromolécules Végétales , 601, rue de la Chimie, BP 53, 38041 Grenoble Cedex 9, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Szarpak-Jankowska
authors.affiliation: Grenoble Alpes University and CEA LETI MlNATEC Campus , 17, avenue des Martyrs, 38054 Grenoble, France.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Jary
authors.affiliation: Grenoble Alpes University and Centre de Recherches sur les Macromolécules Végétales , 601, rue de la Chimie, BP 53, 38041 Grenoble Cedex 9, France.|CNRS and Centre de Recherches sur les Macromolécules Végétales , 601, rue de la Chimie, BP 53, 38041 Grenoble Cedex 9, France.
authors.forename: Rachel
authors.identifier: http://orcid.org/0000-0003-2038-7604
authors.initials: R
authors.lastname: Auzély-Velty
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D004926:Escherichia coli; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D003505:Cyclodextrins
keywords: bacterial capture; cyclodextrin; host−guest interaction; microfluidic chip; quartz crystal microbalance