title: miR-1827 inhibits osteogenic differentiation by targeting IGF1 in MSMSCs.
abstract: We recently reported that maxillary sinus membrane stem cells (MSMSCs) have osteogenic potential. However, the biological mechanisms of bone formation remain unclear. In this study, we investigated the role and mechanisms of microRNAs (miRNAs) in the osteogenic differentiation of MSMSCs. The expression of miRNAs was determined in differentiated MSMSCs by comprehensive miRNA microarray analysis and quantitative RT-PCR (qRT-PCR). We selected miR-1827 for functional follow-up studies to explore its significance in MSMSCs. Here, miR-1827 was found to be up-regulated during osteogenic differentiation of MSMSCs. Over expression of miR-1827 inhibited osteogenic differentiation of MSMSCs in vitro, whereas the repression of miR-1827 greatly promoted cell differentiation. Further experiments confirmed that insulin-like growth factor 1 (IGF1) is a direct target of miR-1827. miR-1827 inhibited osteogenic differentiation partially via IGF1, which in turn is a positive regulator of osteogenic differentiation. Moreover, miR-1827 suppressed ectopic bone formation and silencing of miR-1827 led to increased bone formation in vivo. In summary, this study is the first to demonstrate that miR-1827 can regulate osteogenic differentiation. The increase in miR-1827 expression observed during osteogenesis is likely a negative feedback mechanism, thus offering a potential therapeutic target to address inadequate bone volume for dental implantation through inhibiting miR-1827.
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: ShuangXi
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: JunQuan
authors.initials: J
authors.lastname: Weng
authors.affiliation: Department of Stomatology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: JunBing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: DaiYing
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Prosthetic Dentistry, the First People's Hospital of Yunnan, Kunming, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Rong
authors.affiliation: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.|Guang dong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: SongLing
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002828:Choristoma; D020869:Gene Expression Profiling; D020868:Gene Silencing; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008443:Maxillary Sinus / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C583557:IGF1 protein, human; C561495:MIRN1827 microRNA, human; D035683:MicroRNAs; D007334:Insulin-Like Growth Factor I