title: Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq.
abstract: Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str., 38446 Volos, Greece. Electronic address: emalandrak@uth.gr.
authors.forename: E
authors.initials: E
authors.lastname: Malandrakis
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str., 38446 Volos, Greece.
authors.forename: O
authors.initials: O
authors.lastname: Dadali
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str., 38446 Volos, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Kavouras
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str., 38446 Volos, Greece.
authors.forename: T
authors.initials: T
authors.lastname: Danis
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str., 38446 Volos, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Panagiotaki
authors.affiliation: Department of Applied Hydrobiology, Faculty of Animal Science and Aquaculture, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
authors.forename: H
authors.initials: H
authors.lastname: Miliou
authors.affiliation: Fisheries Research Institute, Hellenic Agricultural Organization-DEMETER, 640 07 Nea Peramos, Kavala, Greece. Electronic address: stsioli@inale.gr.
authors.forename: S
authors.initials: S
authors.lastname: Tsioli
authors.affiliation: Fisheries Research Institute, Hellenic Agricultural Organization-DEMETER, 640 07 Nea Peramos, Kavala, Greece. Electronic address: sorfanid@inale.gr.
authors.forename: S
authors.initials: S
authors.lastname: Orfanidis
authors.affiliation: Oceanlab, University of Aberdeen, Main Street, Newburgh AB41 6AA, UK.
authors.forename: F C
authors.initials: FC
authors.lastname: Küpper
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytokou str., 38446 Volos, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Exadactylos
mesh_terms: D035961:Alismatales / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D009997:Osmotic Pressure; D017423:Sequence Analysis, RNA; D059467:Transcriptome
keywords: Cymodocea nodosa; Differential expression; RNAseq; Transcriptome