title: siRNA Delivery Strategies: A Comprehensive Review of Recent Developments.
abstract: siRNA is a promising therapeutic solution to address gene overexpression or mutations as a post-transcriptional gene regulation process for several pathological conditions such as viral infections, cancer, genetic disorders, and autoimmune disorders like arthritis. This therapeutic method is currently being actively pursued in cancer therapy because siRNA has been found to suppress the oncogenes and address mutations in tumor suppressor genes and elucidate the key molecules in cellular pathways in cancer. It is also effective in personalized gene therapy for several diseases due to its specificity, adaptability, and broad targeting capability. However, naked siRNA is unstable in the bloodstream and cannot efficiently cross cell membranes besides being immunogenic. Therefore, careful design of the delivery systems is essential to fully utilize the potential of this therapeutic solution. This review presents a comprehensive update on the challenges of siRNA delivery and the current strategies used to develop nanoparticulate delivery systems.
authors.affiliation: Use-Inspired Biomaterials & Integrated Nano Delivery (U-BiND) Systems Laboratory, Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA. katyayani.tatiparti@wayne.edu.
authors.forename: Katyayani
authors.initials: K
authors.lastname: Tatiparti
authors.affiliation: Use-Inspired Biomaterials & Integrated Nano Delivery (U-BiND) Systems Laboratory, Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA. samaresh.sau@wayne.edu.
authors.forename: Samaresh
authors.initials: S
authors.lastname: Sau
authors.affiliation: Use-Inspired Biomaterials & Integrated Nano Delivery (U-BiND) Systems Laboratory, Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA. sushilkashaw@gmail.com.|Department of Pharmaceutical Sciences, Dr. Harisingh Gour University (A Central University), Sagar 470003, India. sushilkashaw@gmail.com.
authors.forename: Sushil Kumar
authors.initials: SK
authors.lastname: Kashaw
authors.affiliation: Use-Inspired Biomaterials & Integrated Nano Delivery (U-BiND) Systems Laboratory, Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA. arun.iyer@wayne.edu.|Molecular Therapeutics Program, Barbara Ann Karmanos Cancer Institute, School of Medicine, Wayne State University, Detroit, MI 48201, USA. arun.iyer@wayne.edu.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Iyer
keywords: RNAi; cancer therapy; delivery systems; gene delivery; nanoparticles; siRNA; targeted delivery; tumor