title: Upgrading Laccase Production and Biochemical Properties: Strategies and Challenges.
abstract: Improving laccases continues to be crucial in novel biotechnological developments and industrial applications, where they are concerned. This review breaks down and explores the potential of the strategies (conventional and modern) that can be used for laccase enhancement (increased production and upgraded biochemical properties such as stability and catalytic efficiency). The challenges faced with these approaches are briefly discussed. We also shed light on how these strategies merge and give rise to new options and advances in this field of work. Additionally, this article seeks to serve as a guide for students and academic researchers interested in laccases. This document not only gives basic information on laccases, but also provides updated information on the state of the art of various technologies that are used in this line of investigation. It also gives the readers an idea of the areas extensively studied and the areas where there is still much left to be done. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1015-1034, 2017.
authors.affiliation: Department of Environmental Biotechnology, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca, Morelos, CP 62209, México.
authors.forename: Brandt
authors.identifier: http://orcid.org/0000-0001-7333-4202
authors.initials: B
authors.lastname: Bertrand
authors.affiliation: Department of Environmental Biotechnology, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca, Morelos, CP 62209, México.
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez-Morales
authors.affiliation: Department of Environmental Biotechnology, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Chamilpa, Cuernavaca, Morelos, CP 62209, México.
authors.forename: María R
authors.initials: MR
authors.lastname: Trejo-Hernández
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001669:Biochemical Phenomena; D001709:Biotechnology; D006801:Humans; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D042845:Laccase
keywords: chemical modification; genetic engineering; laccase; laccase improvement; laccase production; native enzymes; traditional strategies