title: Effects of Interfacial Properties of a Surface Modified Surface Plasmon Resonance Chip on Protein Immobilization Performance.
abstract: In order to confirm the correlation between interfacial properties of modified surface plasmon resonance (SPR) chips and their SPR responses to immobilized anti-IgG, SPR chips were modified by mercaptoundecanoic acid, poly(ethylene glycol) diacrylate (PEG), PEG-based copolymer and cyclodextrin coupled PEG using self-assembled or radical polymerization methods. The resulting interfacial properties such as film thickness and hydrophilicity were characterized by AFM, elliptic polarization scanners and contact angle meter. Immobilization of human IgG on the modified chips was achieved by EDC/NHS activation through an amide bond. The association between fixed IgG and free anti-IgG was reflected by the variation of SPR responses and the binding ability was evaluated by Langmuir isotherms. As observed, the adsorption between IgG and anti-IgG was affected by the interfacial properties of different modifiers, such that a chip with a thinner and more hydrophilic layer may result in a higher SPR response, producing a larger adsorption equilibrium constant for protein interaction.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology.
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Jiang
authors.forename: Lishi
authors.initials: L
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zeng
mesh_terms: D000327:Adsorption; D005227:Fatty Acids / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols