title: Remodeling of peripheral nerve ensheathment during the larval-to-adult transition in Drosophila.
abstract: Over the course of a 4-day period of metamorphosis, the Drosophila larval nervous system is remodeled to prepare for adult-specific behaviors. One example is the reorganization of peripheral nerves in the abdomen, where five pairs of abdominal nerves (A4-A8) fuse to form the terminal nerve trunk. This reorganization is associated with selective remodeling of four layers that ensheath each peripheral nerve. The neural lamella (NL), is the first to dismantle; its breakdown is initiated by 6 hours after puparium formation, and is completely removed by the end of the first day. This layer begins to re-appear on the third day of metamorphosis. Perineurial glial (PG) cells situated just underneath the NL, undergo significant proliferation on the first day of metamorphosis, and at that stage contribute to 95% of the glial cell population. Cells of the two inner layers, Sub-Perineurial Glia (SPG) and Wrapping Glia (WG) increase in number on the second half of metamorphosis. Induction of cell death in perineurial glia via the cell death gene reaper and the Diptheria toxin (DT-1) gene, results in abnormal bundling of the peripheral nerves, suggesting that perineurial glial cells play a role in the process. A significant number of animals fail to eclose in both reaper and DT-1 targeted animals, suggesting that disruption of PG also impacts eclosion behavior. The studies will help to establish the groundwork for further work on cellular and molecular processes that underlie the co-ordinated remodeling of glia and the peripheral nerves they ensheath. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1144-1160, 2017.
authors.affiliation: Department of Biology and Center for Neuroscience, Miami University, Oxford, Ohio.
authors.forename: Aswati
authors.identifier: 0000-0002-4447-2945
authors.initials: A
authors.lastname: Subramanian
authors.affiliation: Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
authors.forename: Matthew
authors.initials: M
authors.lastname: Siefert
authors.affiliation: École Polytechnique Fédérale De Lausanne, Lausanne, CH-1015, Switzerland.
authors.forename: Soumya
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Kansas State University, Manhattan, Kansas.
authors.forename: Kumar
authors.initials: K
authors.lastname: Vishal
authors.affiliation: Department of Biology and Center for Neuroscience, Miami University, Oxford, Ohio.
authors.forename: Kayla A
authors.initials: KA
authors.lastname: Bergmann
authors.affiliation: Department of Biology and Center for Neuroscience, Miami University, Oxford, Ohio.
authors.forename: Clay C M
authors.initials: CCM
authors.lastname: Curts
authors.affiliation: Barrington Health and Dental Center, 3401 East Raymond St., Indianapolis, IN, 46203.
authors.forename: Meredith
authors.initials: M
authors.lastname: Dorr
authors.affiliation: The Johns Hopkins School of Medicine, Baltimore, Maryland, 21287.
authors.forename: Camillo
authors.initials: C
authors.lastname: Molina
authors.affiliation: Department of Biology and Center for Neuroscience, Miami University, Oxford, Ohio.
authors.forename: Joyce
authors.initials: J
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001973:Bromodeoxyuridine; D016923:Cell Death; D004167:Diphtheria Toxin / Q000235:genetics / Q000378:metabolism; D004330:Drosophila / Q000033:anatomy & histology* / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D007150:Immunohistochemistry; D007814:Larva / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D008675:Metamorphosis, Biological; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010525:Peripheral Nerves / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism
chemical_list: D004167:Diphtheria Toxin; D029721:Drosophila Proteins; D010446:Peptide Fragments; C029795:diphtheria toxin fragment A; C086973:rpr protein, Drosophila; D001973:Bromodeoxyuridine
keywords: Drosophila; ensheathment; fusion; metamorphosis; remodeling