title: Novel bioactive molecules from Lentzea violacea strain AS 08 using one strain-many compounds (OSMAC) approach.
abstract: A new eudesmane sesquiterpenoid (1), and a new homologue of virginiae butanolide E (2) along with butyl isobutyl phthalate (3) were isolated from, actinomycete-Lentzea violacea strain AS08 isolated from north western Himalayas by stressing on modified one strain-many compounds (OSMAC) method. The structures of the new compounds were elucidated by extensive spectroscopic analyses including 1D, 2D NMR along with HR-ESI-MS and FT-IR data. Herein, a distinctive method was added for inspecting secretory profile of the strain by quantification of extract value of cell free supernatant in different types of culture media fallowed by HPLC profiling of respective extracts, which revealed a highly altered metabolic profile of the strain and formed the base for the selection of media. The compounds 1 and 2 showed moderate activity against Gram negative (MIC ∼32-64µgml-1) in comparison to Gram positive bacterial pathogens. Compound 1 exhibited significant activity in human cancerous cell lines (IC50∼19.2µM).
authors.affiliation: Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India; Academy of Scientific and Innovative Research, CSIR - Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India.
authors.forename: Aehtesham
authors.initials: A
authors.lastname: Hussain
authors.affiliation: Clinical Microbiology and PK/PD Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Muzafar A
authors.initials: MA
authors.lastname: Rather
authors.affiliation: Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu Tawi, 180001, India.
authors.forename: Mohd S
authors.initials: MS
authors.lastname: Dar
authors.affiliation: Medicinal Chemistry Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Mushtaq A
authors.initials: MA
authors.lastname: Aga
authors.affiliation: Medicinal Chemistry Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Nisar
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Aabid
authors.initials: A
authors.lastname: Manzoor
authors.affiliation: Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Jammu Tawi, 180001, India.
authors.forename: Arem
authors.initials: A
authors.lastname: Qayum
authors.affiliation: Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Aiyatullah
authors.initials: A
authors.lastname: Shah
authors.affiliation: Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Saleem
authors.initials: S
authors.lastname: Mushtaq
authors.affiliation: Clinical Microbiology and PK/PD Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India.
authors.forename: Zahoor
authors.initials: Z
authors.lastname: Ahmad
authors.affiliation: Microbial Biotechnology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, Jammu & Kashmir 190005, India; Academy of Scientific and Innovative Research, CSIR - Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India. Electronic address: qphassan@iiim.ac.in.
authors.forename: Qazi P
authors.initials: QP
authors.lastname: Hassan
mesh_terms: D039903:Actinobacteria / Q000737:chemistry* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D045787:Sesquiterpenes, Eudesmane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: C000621976:8-hydroxy-7-(1-hydroxypropan-2-yl)-1,4a-dimethyl-5,6,7,8-tetrahydronaphthalen-2(4aH)-one; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D045787:Sesquiterpenes, Eudesmane
keywords: Actinomycetes; Antitumor and antimicrobial activity; Bioactive compounds; Lentzea violacea