title: Intracellular Cytokines Produced by Stimulated CD3+ Cells from Chronic Myeloid Leukemia Patients.
abstract: Our work examined the production of intracellular interferon (INF)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-2, and IL-4 by in vitro stimulated CD3+ cells from 38 chronic myeloid leukemia (CML) patients. At the time of diagnosis the percentages of cells producing INF-γ, TNF-α, and IL-2 were strongly suppressed compared to those in healthy control subjects. Hematological remission achieved through treatment with tyrosine-kinase inhibitors was associated with a highly significant increase in the ratio of cells producing all 4 cytokines. The percentages of CD3+ cells producing cytokines were dependent on age, more so in CML patients than in healthy controls, and they negatively correlated with the number of leukocytes. Patients with an optimal therapy outcome possessed higher percentages of cytokine-producing CD3+ cells at diagnosis than those with nonoptimal outcomes. This difference was statistically significant in the case of INF-γ-producing cells, and it was on the brink of significance in the case of IL-2-producing cells.
authors.affiliation: Department of Immunology, Institute of Hematology and Blood Transfusion, General University Hospital and First Faculty of Medicine of Charles University, Prague, Czech Republic.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Ptackova
authors.forename: Martina
authors.initials: M
authors.lastname: Petrackova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Hindos
authors.forename: Martina
authors.initials: M
authors.lastname: Duskova
authors.forename: Eva
authors.initials: E
authors.lastname: Hamsikova
authors.forename: Hana
authors.initials: H
authors.lastname: Klamova
authors.forename: Pavla
authors.initials: P
authors.lastname: Pecherkova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Humlova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vonka
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D017252:CD3 Complex / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D047368:Tumor Burden / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D017252:CD3 Complex; D016207:Cytokines; C508594:IL2 protein, human; C508598:IL4 protein, human; D007376:Interleukin-2; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Chronic myeloid leukemia; Interferon-γ; Interleukin-2; Interleukin-4; Intracellular cytokine production; Tumor necrosis factor-α