title: Enterococcus faecalisbacteriocin EntV inhibits hyphal morphogenesis, biofilm formation, and virulence ofCandida albicans.
abstract: Enterococcus faecalis, a Gram-positive bacterium, andCandida albicans, a fungus, occupy overlapping niches as ubiquitous constituents of the gastrointestinal and oral microbiome. Both species also are among the most important and problematic, opportunistic nosocomial pathogens. Surprisingly, these two species antagonize each other's virulence in both nematode infection and in vitro biofilm models. We report here the identification of theE. faecalisbacteriocin, EntV, produced from theentV(ef1097) locus, as both necessary and sufficient for the reduction ofC. albicansvirulence and biofilm formation through the inhibition of hyphal formation, a critical virulence trait. A synthetic version of the mature 68-aa peptide potently blocks biofilm development on solid substrates in multiple media conditions and disrupts preformed biofilms, which are resistant to current antifungal agents. EntV68is protective in three fungal infection models at nanomolar or lower concentrations. First, nematodes treated with the peptide at 0.1 nM are completely resistant to killing byC. albicansThe peptide also protects macrophages and augments their antifungal activity. Finally, EntV68reduces epithelial invasion, inflammation, and fungal burden in a murine model of oropharyngeal candidiasis. In all three models, the peptide greatly reduces the number of fungal cells present in the hyphal form. Despite these profound effects, EntV68has no effect onC. albicansviability, even in the presence of significant host-mimicking stresses. These findings demonstrate that EntV has potential as an antifungal agent that targets virulence rather than viability.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School, Houston, TX 77030.
authors.forename: Carrie E
authors.initials: CE
authors.lastname: Graham
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School, Houston, TX 77030.
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Cruz
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School, Houston, TX 77030; michael.lorenz@uth.tmc.edu danielle.a.garsin@uth.tmc.edu.|The University of Texas Center for Antimicrobial Resistance and Microbial Genomics, The University of Texas Health Science Center at Houston, Houston, TX 77030.
authors.forename: Danielle A
authors.initials: DA
authors.lastname: Garsin
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School, Houston, TX 77030; michael.lorenz@uth.tmc.edu danielle.a.garsin@uth.tmc.edu.|The University of Texas Center for Antimicrobial Resistance and Microbial Genomics, The University of Texas Health Science Center at Houston, Houston, TX 77030.
authors.forename: Michael C
authors.identifier: 0000-0002-7881-8027
authors.initials: MC
authors.lastname: Lorenz
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000378:metabolism* / Q000494:pharmacology*; D018441:Biofilms / Q000254:growth & development*; D017173:Caenorhabditis elegans / Q000382:microbiology; D002176:Candida albicans / Q000187:drug effects* / Q000472:pathogenicity; D002177:Candidiasis / Q000382:microbiology / Q000517:prevention & control; D013293:Enterococcus faecalis / Q000235:genetics / Q000378:metabolism*; D025301:Hyphae / Q000187:drug effects*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009960:Oropharynx / Q000382:microbiology; D000067996:RAW 264.7 Cells; D014774:Virulence
chemical_list: D001430:Bacteriocins
keywords: Candida albicans; Enterococcus faecalis; bacteriocins; biofilms