title: Protein Immobilization onto Cationic Spherical Polyelectrolyte Brushes Studied by Small Angle X-ray Scattering.
abstract: The immobilization of bovine serum albumins (BSA) onto cationic spherical polyelectrolyte brushes (SPB) consisting of a solid polystyrene (PS) core and a densely grafted poly(2-aminoethyl methacrylate hydrochloride) (PAEMH) shell was studied by small-angle X-ray scattering (SAXS). The observed dynamics of adsorption of BSA onto SPB by time-resolved SAXS can be divided into two stages. In the first stage (tens of milliseconds), the added proteins as in-between bridge instantaneously caused the aggregation of SPB. Then BSA penetrated into the brush layer driven by electrostatic attractions, and reached equilibrium in the second stage (tens of seconds). The amount of BSA immobilized onto brush layer reached the maximum when pH was increased to about 6.1 and BSA concentration to 10 g/L. The cationic SPB were confirmed to provide stronger adsorption capacity for BSA compared to anionic ones.
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.|Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH , Hahn-Meitner-Platz 1, 14109 Berlin, Germany.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Paul Scherer Institute , Laboratory for Synchrotron Radiation and Femtochemistry, 5232 Villigen PSI, Switzerland.
authors.forename: Katja
authors.initials: K
authors.lastname: Henzler
authors.affiliation: Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH , Hahn-Meitner-Platz 1, 14109 Berlin, Germany.|Institut für Phzsik, Humboldt-Universität zu Berlin , 12489 Berlin, Germany.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.
authors.forename: Junyou
authors.initials: J
authors.lastname: Wang
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.
authors.forename: Haoya
authors.initials: H
authors.lastname: Han
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.
authors.forename: Zhiming
authors.identifier: http://orcid.org/0000-0001-8462-4483
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: European Synchrotron Radiation Facility , F-38043, Grenoble, France.
authors.forename: Gudrun
authors.initials: G
authors.lastname: Lotze
authors.affiliation: European Synchrotron Radiation Facility , F-38043, Grenoble, France.
authors.forename: Theyencheri
authors.initials: T
authors.lastname: Narayanan
authors.affiliation: Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH , Hahn-Meitner-Platz 1, 14109 Berlin, Germany.|Institut für Phzsik, Humboldt-Universität zu Berlin , 12489 Berlin, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Ballauff
authors.affiliation: State-Key Laboratory of Chemical Engineering, East China University of Science and Technology , Shanghai 200237, People's Republic of China.|Engineering Research Center of Materials Chemical Engineering of Xinjiang Bingtuan, Shihezi University , Xinjiang 832000, People's Republic of China.
authors.forename: Xuhong
authors.identifier: http://orcid.org/0000-0002-1792-8564
authors.initials: X
authors.lastname: Guo
mesh_terms: D002412:Cations / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D000071228:Polyelectrolytes / Q000737:chemistry*; D053838:Scattering, Small Angle; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014965:X-Rays
chemical_list: D002412:Cations; D055767:Immobilized Proteins; D008689:Methacrylates; D000071228:Polyelectrolytes; D012710:Serum Albumin, Bovine