title: Bioactivity of NANOZR Induced by Alkali Treatment.
abstract: In recent years, zirconia has been a recognized implant material in clinical dentistry. In the present study, we investigated the performance of an alkali-modified ceria-stabilized tetragonal ZrO₂ polycrystalline ceramic-based nanostructured zirconia/alumina composite (NANOZR) implant by assessing surface morphology and composition, wettability, bovine serum albumin adsorption rate, rat bone marrow (RBM) cell attachment, and capacity for inducing bone differentiation. NANOZR surfaces without and with alkali treatment served as the control and test groups, respectively. RBM cells were seeded in a microplate with the implant and cultured in osteogenic differentiation medium, and their differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, osteocalcin (OCN) production, calcium deposition, and osteogenic gene expression. The alkali-treated NANOZR surface increased ALP activity, OCN production, calcium deposition, and osteogenesis-related gene expression in attached RBM cells. These data suggest that alkali treatment enhances the osteogenesis-inducing capacity of NANOZR implants and may therefore improve their biointegration into alveolar bone.
authors.affiliation: Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan. nishizaki-m@cc.osaka-dent.ac.jp.
authors.forename: Mariko
authors.initials: M
authors.lastname: Nishizaki
authors.affiliation: Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan. komasa-s@cc.osaka-dent.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komasa
authors.affiliation: Department of Periodotology, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan. taguchi@cc.osaka-dent.ac.jp.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Taguchi
authors.affiliation: Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan. nisizaki@cc.osaka-dent.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishizaki
authors.affiliation: Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan. joji@cc.osaka-dent.ac.jp.
authors.forename: Joji
authors.initials: J
authors.lastname: Okazaki
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D013499:Surface Properties; D017155:Wettability; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015921:Dental Implants; D003764:Dental Materials; D015040:Zirconium; D000537:Aluminum Oxide; C028541:zirconium oxide
keywords: alkali treatment; bioactivity; zirconia/alumina nano-composite