title: Metastasized murine oral squamous cell carcinoma cells induce intratumoral polymorphonuclear myeloid derived suppressor cells.
abstract: Myeloid derived suppressor cells (MDSCs) localize to hematopoietic organs and peripheral blood during inflammation or tumor tissues and lymph nodes in the presence of a tumor. However, whether there are differences in MDSCs found in the primary tumor and metastases is unknown. In the present study, we established a cell line of metastasized tumor cells to a lymph node, L5-11, which were derived from the Sq-1979 mouse buccal mucosa squamous cell carcinoma cell line. We then analyzed tumor immunogenicity, especially with regard to MDSCs, to clarify the differences between the primary tumor and metastases, using an isogenic heterotopic tumor transplantation model. Our data showed that the population of intratumoral MDSCs, especially polymorphonuclear MDSCs in the lymph node metastasis model were significantly increased compared with syngeneic grafts from the primary cell line Sq-1979 after 21 days. Furthermore, we identified that the lymph node metastasis cell line had increased expression of genes that promote the expansion of MDSCs, tumor growth and metastasis. Hence, these data suggest that tumor immunosuppression can occur via activation of MDSCs. However, further examination is required to clarify whether all or a subset of these factors from the lymph node metastasis tumor cells are required to induce intratumoral MDSCs.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sumi
authors.affiliation: Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Umemura
authors.affiliation: Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Takayama
authors.affiliation: Department of Natural and Medicinal Chemistry, Faculty of Pharmaceutical Sciences Aomori University, Aomori 030-0943, Japan.
authors.forename: Emika
authors.initials: E
authors.lastname: Ohkoshi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Adachi
authors.affiliation: Chemistry Laboratory Department of Management and Information Studies, Asahi University School of Business Administration, Gifu 501-0296, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Mizuno-Kamiya
authors.affiliation: Department of Oral and Maxillofacial Surgery, Division of Reparative and Regenerative Medicine, Institute of Medical Science, Mie University Graduate School of Medicine, Tsu 514-8507, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Inagaki
authors.affiliation: Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Kawaki
authors.affiliation: Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sumitomo
authors.affiliation: Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu 501‑0296, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kondoh
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D009062:Mouth Neoplasms / Q000473:pathology*; D000072737:Myeloid-Derived Suppressor Cells / Q000473:pathology*; D009368:Neoplasm Transplantation; D011379:Prognosis; D016042:Transplantation, Heterotopic