title: CD33, CD96 and Death Associated Protein Kinase (DAPK) Expression Are Associated with the Survival Rate and/or Response to the Chemotherapy in the Patients with Acute Myeloid Leukemia (AML).
abstract: BACKGROUND Leukemia stem cells (LSC) are involved in the incidence, drug resistance, and relapse of leukemia while LSC-related antigen CD33, CD96, and DAPK expression in AML and its prognosis is still unclear. This study explored LSC-related antigens expression in acute myeloid leukemia (AML) and its prognosis. MATERIAL AND METHODS A total of 156 cases of AML patients were enrolled in the experiment. The expression of CD33, CD96, and DAPK in CD34+CD38-CD123+ LSC were tested by flow cytometry. The survival curve was established using the Kaplan-Meier method. RESULTS Among different subtypes of AML, the positive rate of CD33 was M3> M5> M1> M2> M4; for CD96 it was M5> M4> M2> M3> M1; and for DAPK it was M3> M2> M5> M4> M1. After chemotherapy, the response rate in CD33 and CD96 high expression groups, and DAPK low expression group was significantly lower than the groups with CD33 low expression, CD96 low expression, and DAPK high expression. The median survival time in the CD33 high expression group was markedly lower than the CD33 low expression group (36.5 months). The CD96 high expression group exhibited obviously shorter median survival time than the CD96 low expression group. The DAPK high expression group exhibited longer median survival time than the DAPK low expression group. CONCLUSIONS CD33 and CD96 overexpression, and DAPK downregulation in the LSC of AML patients were associated with poor chemotherapy effect and prognosis, and higher recurrence rate.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors.forename: Dandan
authors.initials: D
authors.lastname: Yao
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China (mainland).
authors.forename: Haibin
authors.initials: H
authors.lastname: Dai
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D054316:Biomarkers, Pharmacological; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism*; D064550:Death-Associated Protein Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000378:metabolism; D011379:Prognosis; D043562:Receptors, G-Protein-Coupled; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate
chemical_list: C095004:ADGRE5 protein, human; D015703:Antigens, CD; D054316:Biomarkers, Pharmacological; C571163:CD33 protein, human; D043562:Receptors, G-Protein-Coupled; D063268:Sialic Acid Binding Ig-like Lectin 3; D064550:Death-Associated Protein Kinases