title: Molecular diversity of Pakistani mango (Mangifera indica L.) varieties based on microsatellite markers.
abstract: Understanding the genetic diversity of different Pakistani mango varieties is important for germplasm management and varietal characterization. Microsatellites are efficient and highly polymorphic markers for comparative genome mapping, and were used in the present study to determine the genetic relatedness and variability among 15 indigenous mango cultivars (Mangifera indica L.). Overall, 181 bands were produced using 12 simple sequence repeat (SSR) primers. Out of the 12 primers used, 10 were polymorphic and two were monomorphic. Genetic relatedness among cultivars was assessed by constructing a dendrogram using the unweighted pair group method of arithmetic means. The accessions exhibited coefficients of similarity ranging from 75 to 100%, indicating the frequent use of only a few parent cultivars and the presence of inbreeding. The primers used in the present study were found to be valuable for identifying genetic relationships among mango cultivars.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: T
authors.initials: T
authors.lastname: Nazish
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: G
authors.initials: G
authors.lastname: Shabbir
authors.affiliation: Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Ali
authors.affiliation: College of Agriculture, Sub-Campus Layyah, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Sami-Ul-Allah
authors.affiliation: Department of Plant Breeding and Genetics, University College of Agriculture, Islamia University, Bahawalpur, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Naeem
authors.affiliation: Department of Plant Breeding and Genetics, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Javed
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Batool
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: H
authors.initials: H
authors.lastname: Arshad
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: S B
authors.initials: SB
authors.lastname: Hussain
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: K
authors.initials: K
authors.lastname: Aslam
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: R
authors.initials: R
authors.lastname: Seher
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Tahir
authors.affiliation: Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan muhammadbabar@bzu.edu.pk.
authors.forename: M
authors.initials: M
authors.lastname: Baber
mesh_terms: D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D031022:Mangifera / Q000235:genetics*; D018895:Microsatellite Repeats; D010154:Pakistan; D010802:Phylogeny; D000069600:Plant Breeding; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers