title: Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources.
abstract: Oligodendrocytes are part of the glial cells located in the central nervous system, capable of providing trophic support to neurons and ensheathing their axons. These cells can become dysfunctional under pathologic condition. Rodent and human pluripotent stem cells are inexhaustible sources for producing oligodendrocytes that can be used for disease modeling and cell replacement therapy studies. They also offer many opportunities to model the contribution of oligodendrocytes in non-genetic disorders such as multiple system atrophy. In this method article, we provide robust and reproducible differentiation protocols to obtain oligodendrocyte progenitor cells and purify them using fluorescence activated cell sorting.
authors.affiliation: Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, Sweden; Strategic Research Area MultiPark, Lund University, 22184 Lund, Sweden; Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Djelloul
authors.affiliation: Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, Sweden; Strategic Research Area MultiPark, Lund University, 22184 Lund, Sweden; Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Carla
authors.initials: C
authors.lastname: Azevedo
authors.affiliation: Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, Sweden; Strategic Research Area MultiPark, Lund University, 22184 Lund, Sweden; Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Pomeshchik
authors.affiliation: MultiPark Strategic Research Area, Cellomics and Flow Cytometry Core Facility, Department of Experimental Medical Science, Lund University, BMC B12, 22184 Lund, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Hammarberg
authors.affiliation: Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, BMC A10, Lund University, 22184 Lund, Sweden; Strategic Research Area MultiPark, Lund University, 22184 Lund, Sweden; Lund Stem Cell Center, Lund University, 22184 Lund, Sweden. Electronic address: Laurent.roybon@med.lu.se.
authors.forename: Laurent
authors.initials: L
authors.lastname: Roybon
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D006801:Humans; D004676:Myelin Basic Protein / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein
keywords: FACS; O4; OPC purification; Oligodendrocytes; PDGFRα/CD140; Pluripotent stem cells