title: [Effect of androgen receptor on IgG expression, proliferation and migration of prostate cancer cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of androgen receptor (AR) on IgG protein expression and the proliferation and migration of prostate cancer cells.
  
  
  METHODS
  Western blotting was used to detect the expression of AR protein and IgG in androgen-dependent prostate cancer LNCap cells and castration-resistant prostate cancer PC-3 cells. In AR-overexpressing cells (PC-3-AR cells) established by transfecting PC-3 with AR gene (pCDNA3.1) and LNCap cells with small interfering RNA-mediated AR silencing (LNCap-siAR cells) were analyzed for expressions of AR protein and IgG with Western blotting; the expression of IgG mRNA was detected by Q-PCR, and the cell proliferation and migration were assessed with MTT assay and wound healing assay, respectively.
  
  
  RESULTS
  Compared with PC-3 cells, LNCap cells expressed a higher level of AR protein and a lower level of IgG (P<0.05). PC-3-AR cells showed attenuated proliferation and migration with a lowered expression of IgG (P<0.01), while LNCap-siAR cells showed enhanced proliferation and migration with increased expression of IgG (P<0.01).
  
  
  CONCLUSIONS
  The expression of AR is inversely correlated with IgG and is associated with the proliferation and migration of prostate cancer cells in vitro.
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail: 632609807@qq.com.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Deng
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.forename: Ying-Ke
authors.initials: YK
authors.lastname: Zeng
authors.forename: Kai-Hui
authors.initials: KH
authors.lastname: Wu
authors.forename: Chen
authors.initials: C
authors.lastname: Tang
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D011944:Receptors, Androgen / Q000378:metabolism*
chemical_list: D007074:Immunoglobulin G; D011944:Receptors, Androgen