title: Curcumin inhibits prostate cancer by targeting PGK1 in the FOXD3/miR-143 axis.
abstract: OBJECTIVE
  Curcumin is a potent antitumor agent. The objective of this study was to explore the interaction between curcumin and PGK1, an oncogene in the FOXD3/miR-143 axis, in prostate cancer therapy.
  
  
  METHODS
  MiRNA microarray analysis was used to identify miRNAs upregulated by curcumin treatment. MiR-143 was dramatically upregulated by curcumin. Cells were treated with antimiR-143 in combination to curcumin, followed by examining cell viability and migration. Bioinformatics analysis was used to investigate target genes of miR-143. The interaction between miR-143 and PGK1 was evaluated with dual-luciferase assay. Since FOXD3 is important in the regulation of miR-143, we explored whether curcumin regulated FOXD3 expression. FOXD3 was also ectopically overexpressed to investigate its effects on curcumin's regulation of miR-143.
  
  
  RESULTS
  Curcumin treatment significantly upregulated miR-143 and decreased prostate cancer cell proliferation and migration. Those effects were attenuated by anti-miR-143 transfection. Both miR-143 overexpression and curcumin treatment inhibited PGK1 expression and ectopic expression of PGK1 antagonized curcumin's antitumor effects. FOXD3 was upregulated by miR-143. Ectopic expression of FOXD3 synergized with curcumin in upregulating miR-143 expression.
  
  
  CONCLUSIONS
  Curcumin inhibits prostate cancer by upregulating miR-143. PGK1 is downregulated by miR-143, and FOXD3 upregulation is essential for the antitumor effect of curcumin.
authors.affiliation: Surgical Department I (Urology Department), Shanghai University of Traditional Chinese Medicine Affiliated LONGHUA Hospital, No. 725 Wanping Road South, Xuhui District, Shanghai, 200032, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Cao
authors.affiliation: Oncology Department of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine Affiliated PUTUO Hospital, No. 164 Lanxi Road, Putuo District, Shanghai, 200062, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Yu
authors.affiliation: Surgical Department I (Urology Department), Shanghai University of Traditional Chinese Medicine Affiliated LONGHUA Hospital, No. 725 Wanping Road South, Xuhui District, Shanghai, 200032, China.
authors.forename: Yigeng
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Surgical Department I (Urology Department), Shanghai University of Traditional Chinese Medicine Affiliated LONGHUA Hospital, No. 725 Wanping Road South, Xuhui District, Shanghai, 200032, China. chenleijoe@126.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Oncology Department of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine Affiliated PUTUO Hospital, No. 164 Lanxi Road, Putuo District, Shanghai, 200062, China. liangfang9876@126.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Liang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019295:Computational Biology; D003474:Curcumin / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000096:biosynthesis / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018014:Gene Transfer Techniques; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000187:drug effects*; D046228:Microarray Analysis; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis / Q000187:drug effects*; D011471:Prostatic Neoplasms / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents; C503514:FOXD3 protein, human; D051858:Forkhead Transcription Factors; C542359:MIRN143 microRNA, human; D035683:MicroRNAs; C577282:PGK1 protein, human; D010735:Phosphoglycerate Kinase; D003474:Curcumin
keywords: Curcumin; FOXD3/miR-143; PGK1; Prostate cancer