title: Changes in SCD gene DNA methylation after bariatric surgery in morbidly obese patients are associated with free fatty acids.
abstract: Stearoyl CoA Desaturase-1 (SCD) is considered as playing an important role in the explanation of obesity. The aim of this study was to evaluate whether the DNA methylation SCD gene promoter is associated with the metabolic improvement in morbidly obese patients after bariatric surgery. The study included 120 subjects with morbid obesity who underwent a laparoscopic Roux-en Y gastric by-pass (RYGB) and a control group of 30 obese subjects with a similar body mass index (BMI) to that found in morbidly obese subjects six months after RYGB. Fasting blood samples were obtained before and at six months after RYGB. DNA methylation was measured by pyrosequencing technology. DNA methylation levels of the SCD gene promoter were lower in morbidly obese subjects before bariatric surgery but increased after RYGB to levels similar to those found in the control group. Changes of DNA methylation SCD gene were associated with the changes of free fatty acids levels (r = -0.442, p = 0.006) and HOMA-IR (r = -0.249, p = 0.035) after surgery. RYGB produces an increase in the low SCD methylation promoter levels found in morbidly obese subjects. This change of SCD methylation levels is associated with changes in FFA and HOMA-IR.
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Málaga, Spain.
authors.forename: Sonsoles
authors.initials: S
authors.lastname: Morcillo
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain.
authors.forename: Gracia Mª
authors.initials: GM
authors.lastname: Martín-Núñez
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.|CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Málaga, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: García-Serrano
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Gutierrez-Repiso
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Rodriguez-Pacheco
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.|CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Málaga, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Valdes
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Gonzalo
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.|CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Málaga, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Rojo-Martinez
authors.affiliation: Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Moreno-Ruiz
authors.affiliation: Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Rodriguez-Cañete
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Málaga, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Tinahones
authors.affiliation: CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Málaga, Spain.|Unidad de Gestión Clínica de Aparato Digestivo, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Fuentes
mesh_terms: D000328:Adult; D050110:Bariatric Surgery; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics* / Q000378:metabolism* / Q000601:surgery; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*
chemical_list: D005230:Fatty Acids, Nonesterified; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase