title: Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system.
abstract: Our previous study found that co-culture with human vascular endothelial cells (HMVECs) is beneficial for dorsal root ganglion cells (DRGCs). The goal of the present study is to investigate whether co-culture with HMVECs could promote the development of DRGCs, and whether this effect is induced by the secretion of BDNF by HMVECs. DRGCs were mono-cultured, co-cultured with HMVECs or co-cultured with HMVECs that pre-transfected with BDNF siRNA, the expression of neurite formation and branching factors were determined. The results showed that transfecting with BDNF siRNA inhibited BDNF expression and reduced BDNF secretion. Co-culture with HMVECs increased the expression of Etv4, Etv5, FN-L, FN-M, and GAP-43 in DRGCs that accompanied by the activation of ERK pathway. However, these changes were all reversed by the inhibition of BDNF in HMVECs. In conclusion, our data demonstrate that HMVECs potentiated DRGCs development at least partly by the secretion of BDNF in the co-culture system.
authors.affiliation: a Department of Orthopedics , Shengjing Hospital of China Medical University , Shenyang , People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: b Department of Nephrology , The First Affiliated Hospital of China Medical University , Shenyang , People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: a Department of Orthopedics , Shengjing Hospital of China Medical University , Shenyang , People's Republic of China.
authors.forename: Honghao
authors.initials: H
authors.lastname: Yu
authors.affiliation: a Department of Orthopedics , Shengjing Hospital of China Medical University , Shenyang , People's Republic of China.
authors.forename: Chunhou
authors.initials: C
authors.lastname: An
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D061251:Primary Cell Culture; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004268:DNA-Binding Proteins; C540571:Etv4 protein, mouse; C513242:Etv5 protein, mouse; D019922:GAP-43 Protein; D050783:Proto-Oncogene Proteins c-ets; D034741:RNA, Small Interfering; D014157:Transcription Factors; C579455:BDNF protein, human
keywords: brain-derived neurotrophic factor; co-culture; dorsal root ganglion cells; neurite outgrowth