title: How the presence of a small molecule affects the complex coacervation between lactoferrin and β-lactoglobulin.
abstract: Heteroprotein complex coacervation corresponds to the formation of two liquid phases in equilibrium induced by the interaction of two oppositely charged proteins. The more concentrated phase known as coacervate phase, has attracted interest from several fields of science due to its potential applications for example for encapsulation and delivery of bioactives. Prior such application, it is necessary to understand how the presence of small ligands affects the complex coacervation. In this work, we report on the interaction of small ligand with individual proteins β-lactoglobulin (β-LG) and lactoferrin (LF) and consequences on their complex coacervation. ANS (8-Anilinonaphthalene-1-sulfonic acid), a fluorescent probe, was used as model ligand. While ANS did not interact with β-LG, it presented two sets of binding sites with LF inducing its self-aggregation. Depending on its concentration, ANS modulated the shape of β-LG-LF macromolecular assembly. Coacervates were observed for ANS/LF molar ratio <25 against amorphous aggregates for higher ANS/LF molar ratios. A maximum loading capacity of around 40mg of ANS per gram of LF in the formed heteroprotein coacervates was reached.
authors.affiliation: STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France; Laboratory of Research in Milk Products, Universidade Federal de Viçosa, BR-36570 Viçosa, Brazil.
authors.forename: Guilherme M
authors.initials: GM
authors.lastname: Tavares
authors.affiliation: STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Croguennec
authors.affiliation: STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France.
authors.forename: Pascaline
authors.initials: P
authors.lastname: Hamon
authors.affiliation: Laboratory of Research in Milk Products, Universidade Federal de Viçosa, BR-36570 Viçosa, Brazil.
authors.forename: Antônio F
authors.initials: AF
authors.lastname: Carvalho
authors.affiliation: STLO, UMR1253, INRA, Agrocampus Ouest, 35000 Rennes, France. Electronic address: said.bouhallab@rennes.inra.fr.
authors.forename: Saïd
authors.initials: S
authors.lastname: Bouhallab
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D007781:Lactoferrin / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D055672:Static Electricity
chemical_list: C515594:8-anilino-1-naphthalenesulfonic acid; D000817:Anilino Naphthalenesulfonates; D007782:Lactoglobulins; D007781:Lactoferrin
keywords: ANS; Binding; Complex coacervation; ITC; Lactoferrin; β-Lactoglobulin