title: Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity.
abstract: BACKGROUND
  Behçet disease (BD) is a relapsing inflammatory disease with increased production of inflammatory cytokines in peripheral blood mononuclear cells (PBMCs); however, the underlying molecular mechanisms are not well known.
  
  
  OBJECTIVE
  To analyze whether the differential expression of transcription factors is involved in the increased tumor necrosis factor (TNF)-α and interleukin (IL)-6 production by PBMCs of BD patients compared to healthy controls (HCs).
  
  
  METHODS
  Expression of transcription factors was examined by real-time reverse transcriptase-polymerase chain reaction and western blotting. Cytokine production by CD11b+ cells transfected with siRNAs against transcription factors was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  In the absence of lipopolysaccharide stimulation, the transcript level of CCAAT-enhancer-binding proteins (C/EBP) β was increased in PBMCs from patients with active BD compared to that in PBMCs from patients with stable BD. The C/EBPδ transcript level was higher in PBMCs from patients with active BD than in those from HCs. The activating transcription factor 3 (ATF3) transcript level was increased in PBMCs from patients with stable BD compared to that in PBMCs from HCs. siRNAs targeting C/EBPβ and C/EBPδ significantly reduced the production of IL-6 and TNF-α in lipopolysaccharide-stimulated CD11b+ cells from patients with BD as well as from HCs.
  
  
  CONCLUSIONS
  We found differential expression of C/EBPβ, C/EBPδ, and ATF3 in PBMCs from patients with BD depending on disease activity, indicating the involvement of these molecules in BD pathogenesis.
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, Korea.
authors.forename: Min-Yeong
authors.initials: MY
authors.lastname: Woo
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Yun
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, Korea.
authors.forename: Kyongmin
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Eun-So
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, Korea.
authors.forename: Sun
authors.initials: S
authors.lastname: Park
keywords: Behcet syndrome; CCAAT-enhancer-binding proteins; Gene expression; Interleukin-6; Tumor necrosis factor-alpha