title: JMJ24 antagonizes histone H3K9 demethylase IBM1/JMJ25 function and interacts with RNAi pathways for gene silencing.
abstract: Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work, we analyzed the function of a closely related homolog of IBM1/JMJ25, namely JMJ24. We show that jmj24 mutations produced a number of subtle developmental defects, while affecting only a relatively small number of genes at the vegetative stage. Interestingly, jmj24 mutation could complement plant growth defects and expression changes caused by the ibm1 mutation. In addition, we show that JMJ24 may synergistically interact with the RNAi pathways involving siRNAs. The present data suggest that JMJ24 may have a function to counteract IBM1/JMJ25 in gene expression and may cooperate with RNAi pathways for gene silencing.
authors.affiliation: Institute of Plant Sciences Paris Saclay (IPS2), Bâtiment 630, Université Paris-Sud 11, Université Paris-Saclay, 91405 Orsay, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Audonnet
authors.affiliation: Institute of Plant Sciences Paris Saclay (IPS2), Bâtiment 630, Université Paris-Sud 11, Université Paris-Saclay, 91405 Orsay, France.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Institute of Plant Sciences Paris Saclay (IPS2), Bâtiment 630, Université Paris-Sud 11, Université Paris-Saclay, 91405 Orsay, France. Electronic address: dao-xiu.zhou@up-sud.fr.
authors.forename: Dao-Xiu
authors.initials: DX
authors.lastname: Zhou
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics*; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D006657:Histones / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics*; D009154:Mutation; D034622:RNA Interference; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; C526313:IBM1 protein, Arabidopsis; C000608068:JMJ24 protein, Arabidopsis; D056484:Jumonji Domain-Containing Histone Demethylases