title: Regulation of miR-21 expression in human melanoma via UV-ray-induced melanin pigmentation.
abstract: Excessive environmental ultraviolet (UV) radiation produces genetic mutations that can lead to skin cancer. This study was designed to assess the potential inhibitory activity of microRNA-21 (miR-21) on the UV irradiation-stimulated melanogenesis signal pathway in melanoma cells. The molecular mechanism of miR-21-induced inhibitory activity on UV-ray-stimulated melanogenesis-regulating proteins was examined in A375.S2 human melanoma and B16F10 mouse melanoma cells. UV irradiation for 30 min induced melanogenesis signal pathway by increasing melanin production and the number of A375.S2 cells. Similarly, UV radiation increased the expression of α-melanocyte-stimulating hormone (α-MSH) protein and decreased the melanogenesis-regulating signal, such as EGFR and Akt phosphorylation. Notably, miR-21 overexpression in UV-ray-stimulated A375.S2 cells decreased α-MSH expression and increased EGFR and Akt phosphorylation levels. Furthermore, miR-21 on UV-ray- induced melanogenesis was down-regulated by the Akt inhibitor and the EGFR inhibitor (Gefitinib). Results suggest that the suppressive activity of miR-21 on UV-ray-stimulated melanogenesis may involve the down-regulation of α-MSH and the activation in both of EGFR and Akt.
authors.affiliation: Department of Nursing, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan.|Graduate Institute of Biotechnology, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan.
authors.forename: Kuan-Yu
authors.initials: KY
authors.lastname: Lin
authors.affiliation: Division of Neurosurgery, Department of Surgery, Changhua Christian Hospital, Changhua, 50094, Taiwan.|School of Medicine, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.
authors.forename: Chien-Min
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Graduate Institute of Biotechnology, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan.|Department of Neurology, Chang-Gung Memorial Hospital, Linkou, Taoyuan, 33305, Taiwan.
authors.forename: Cheng-You
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Division of Neurosurgery, Department of Surgery, Changhua Christian Hospital, Changhua, 50094, Taiwan.|School of Medicine, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.|Department of Biomedical Engineering, Chung Yuan Christian University, Taoyuan, 32023, Taiwan.
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Cheng
authors.affiliation: Dermatology, Feng Yuan Hospital, Ministry of Health and Welfare, Taichung, 42055, Taiwan.
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Wu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015536:Down-Regulation; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077156:Gefitinib; D006801:Humans; D008543:Melanins / Q000378:metabolism*; D008545:Melanoma / Q000235:genetics / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010766:Phosphorylation; D010858:Pigmentation; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D011799:Quinazolines / Q000494:pharmacology; D015398:Signal Transduction; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D000521:alpha-MSH / Q000378:metabolism
chemical_list: C524940:MIRN21 microRNA, human; C531950:MIRN21 microRNA, mouse; D008543:Melanins; D035683:MicroRNAs; D011799:Quinazolines; D000521:alpha-MSH; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; D000077156:Gefitinib
keywords: Akt; EGFR; Gefitinib; melanogenesis; miR-21