title: Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions.
abstract: Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.
authors.affiliation: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors.forename: Elias
authors.initials: E
authors.lastname: Uhlin
authors.affiliation: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors.forename: Harriet
authors.initials: H
authors.lastname: Rönnholm
authors.affiliation: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors.forename: Kelly
authors.initials: K
authors.lastname: Day
authors.affiliation: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Kele
authors.affiliation: Center of Neurodevelopmental Disorders at Karolinska Institutet, Pediatric Neuropsychiatry Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden; Center of Psychiatry Research, Stockholm County Council, Stockholm, Sweden.
authors.forename: Kristiina
authors.initials: K
authors.lastname: Tammimies
authors.affiliation: Center of Neurodevelopmental Disorders at Karolinska Institutet, Pediatric Neuropsychiatry Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden; Center of Psychiatry Research, Stockholm County Council, Stockholm, Sweden.
authors.forename: Sven
authors.initials: S
authors.lastname: Bölte
authors.affiliation: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden. Electronic address: anna.falk@ki.se.
authors.forename: Anna
authors.initials: A
authors.lastname: Falk
mesh_terms: D002460:Cell Line; D065150:Cellular Reprogramming; D003470:Culture Media / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D029082:Sendai virus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014430:Twins, Monozygotic
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D014157:Transcription Factors