title: The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation.
abstract: The insect short neuropeptide F (sNPF) family has been shown to modulate diverse physiological processes, such as feeding, appetitive olfactory behavior, locomotion, sleep homeostasis and hormone release. In this study, we identified the sNPF (BdsNPF) and its receptor (BdsNPFR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis (Hendel). Afterwards, the receptor cDNA was functionally expressed in Chinese hamster ovary cell lines. Activation of BdsNPFR by sNPF peptides caused an increase in intracellular calcium ions, with a 50% effective concentration values at the nanomolar level. As indicated by qPCR, the BdsNPF and BdsNPFR transcripts were mainly detected in the central nervous system and antennae, and they showed significantly starvation-induced expression patterns. Furthermore, we found that the starved flies had an increased electroantennogram response compared to the normally fed flies. However, this enhanced olfactory sensitivity was reversed when we decreased the expression of BdsNPF by double-stranded RNA injection in adults. We concluded that sNPF plays an important role in modulating the olfactory sensitivity of B. dorsalis upon starvation. Our results will facilitate the understanding of the regulation of early olfactory processing in B. dorsalis.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. Electronic address: jhb8342@swu.edu.cn.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. Electronic address: shunhuagui@163.com.
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Gui
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. Electronic address: xuli940208@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. Electronic address: pyx20830@163.com.
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Pei
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China; Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. Electronic address: guy.smagghe@ugent.be.
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. Electronic address: wangjinjun@swu.edu.cn.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D003412:Cricetulus; D055724:Electrophysiological Phenomena; D005508:Food Deprivation; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology / Q000502:physiology*; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism*; D012903:Smell / Q000502:physiology; D033621:Tephritidae / Q000187:drug effects / Q000502:physiology*
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides; D018013:Receptors, Neuropeptide; C076101:neuropeptide F; D002118:Calcium
keywords: G-protein coupled-receptor; Olfaction sensing; Oriental fruit fly; Short neuropeptide F; Starvation