title: Different nitrogen sources change the transcriptome of welan gum-producing strain Sphingomonas sp. ATCC 31555.
abstract: To reveal effects of different nitrogen sources on the expressions and functions of genes in Sphingomonas sp. ATCC 31555, it was cultivated in medium containing inorganic nitrogen (IN), organic nitrogen (ON), or inorganic-organic combined nitrogen (CN). Welan gum production and bacterial biomass were determined, and RNA sequencing (RNA-seq) was performed. Differentially expressed genes (DEGs) between the different ATCC 31555 groups were identified, and their functions were analyzed. Welan gum production and bacterial biomass were significantly higher in the ON and CN groups compared with those in the IN group. RNA-seq produced 660 unigenes, among which 488, 731, and 844 DEGs were identified between the IN vs. ON, IN vs. CN, and ON vs. CN groups, respectively. All the DEGs were related significantly to metabolic process and signal transduction. DEGs between the IN vs. CN and ON vs. CN groups were potentially associated with bacterial chemotaxis. Real-time PCR confirmed the expressions of selected DEGs. Organic nitrogen led to higher bacterial biomass and welan gum production than inorganic nitrogen, which might reflect differences in gene expression associated with metabolic process, signal transduction, and bacterial chemotaxis induced by different nitrogen sources.
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China.|National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hanghzou, 310018, China.
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Nie
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Jiangsu Rayguang Biotech Company, Ltd., Wuxi, Jiangsu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China. zhanxiaobeiz@hotmail.com.|National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China. zhanxiaobeiz@hotmail.com.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Zhan
mesh_terms: D001483:Base Sequence; D018533:Biomass; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D009584:Nitrogen / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D020578:Sphingomonas / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics
chemical_list: D011135:Polysaccharides, Bacterial; C068570:welan; D009584:Nitrogen
keywords: Differentially expressed gene; Sphingomonas; Transcriptome; Welan gum