title: Solute carrier organic anion transporter family member 4A1 (SLCO4A1) as a prognosis marker of colorectal cancer.
abstract: OBJECTIVE
  Solute carrier organic anion transporter family member 4A1 (SLCO4A1) is involved in the transport of various compounds, including sugars, bile salts, organic acids, metal ions, amine compounds, and estrogen. SLCO4A1 is highly expressed in several cancers and a gender bias has been observed in colorectal cancer (CRC). We investigated SLCO4A1 expression, its prognostic value in patients with CRC, and its role in CRC cell proliferation and metastasis.
  
  
  METHODS
  SLCO4A1 expression was assessed by immunohistochemistry (IHC) on specimens from 84 patients with CRC. The association of SLCO4A1 expression with clinicopathological features was examined. To confirm the biological role of SLCO4A1 in CRC, four CRC cell lines expressing SLCO4A1 were used and SLCO4A1 expression was knocked down by siRNA. Cell proliferation, MTT, migration, invasion, and semisolid agar colony formation assays were performed.
  
  
  RESULTS
  SLCO4A1 was overexpressed in 32% of the CRC samples. SLCO4A1 overexpression and pathologic T stage were independent prognostic factors of decreased survival (P = 0.021). Kaplan-Meier analysis indicated a decreased cumulative survival for patients highly expressing SLCO4A1 compared to patients showing low SLCO4A1 expression (Log-rank test, P = 0.025). In cell lines, SLCO4A1 knockdown resulted in a significant decrease of viability, invasion, and migration when compared to control cells. Semisolid colony formation assay indicated that SLCO4A1-knocked down cells presented poor carcinogenic abilities compared to control cells.
  
  
  CONCLUSIONS
  SLCO4A1 may be a valuable marker of poor prognostic for CRC. Furthermore, SLCO4A1 plays an important role in CRC cell proliferation, migration, invasion, and carcinogenesis.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.|Department of Medicine, The Graduate School of Yonsei University, Seoul, Republic of Korea.
authors.forename: Myung Jin
authors.initials: MJ
authors.lastname: Ban
authors.affiliation: Soonchunhyang Medical Science Research Institute, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Ji
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.
authors.forename: Chi-Kyu
authors.initials: CK
authors.lastname: Lee
authors.affiliation: Department of Oncology, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.
authors.forename: Sang Byung
authors.initials: SB
authors.lastname: Bae
authors.affiliation: Department of Oncology, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.
authors.forename: Han Jo
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Surgery, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Ahn
authors.affiliation: Department of Surgery, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea.
authors.forename: Moon Soo
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Surgery, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea. ssurge@schmc.ac.kr.
authors.forename: Moo-Jun
authors.initials: MJ
authors.lastname: Baek
authors.affiliation: Soonchunhyang Medical Science Research Institute, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea. juny1024@schmc.ac.kr.|Department of Pathology, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea. juny1024@schmc.ac.kr.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Jeong
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D027361:Organic Anion Transporters / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D027361:Organic Anion Transporters; C498174:SLCO4A1 protein, human
keywords: Cell proliferation; Colorectal cancer; Metastasis; Prognostic value; Solute carrier organic anion transporter family member 4A1