title: Highly Sensitive Detection of Bisphenol A by NanoAptamer Assay with Truncated Aptamer.
abstract: For the sensitive quantification of bisphenol A (BPA), we have developed NanoAptamer assay, which employs aptamer and complementary signaling DNA, a set of quantum dots (QD), and magnetic beads (MBs). Signaling DNA-QD655was tethered to MB-QD565via the aptamer. The affinity of the aptamer to BPA resulted in the release of the signaling DNA-QD655from the complex and hence the corresponding decrease in the QD655fluorescence measurement signal. Three new aptamers (23, 58, and 24-mer) were designed via truncation of the reference aptamer (73-mer). The sensitivity and selectivity of each aptamer for BPA detection via NanoAptamer assay were investigated. One of the truncated aptamers (24-mer) has shown a significantly better performance (limit of detection, LOD, 0.17 pg/mL) than the reference 73-mer aptamer (LOD, 570 pg/mL). It has also shown the best selectivity for BPA detection over BPA analogues (i.e., bisphenol B, bisphenol C, and diethylstilbestrol). It corresponded to a normalized fluorescence change of 33.7% at the environmentally relevant concentration of 1 ng/mL (1 ppb) BPA; however, the analogues remained unchanged (2.3-3.9%).
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University , Seoul 03760, Republic of Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Lee
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University , Seoul 03760, Republic of Korea.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Lim
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University , Seoul 03760, Republic of Korea.
authors.forename: Sang-Don
authors.initials: SD
authors.lastname: Lee
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University , Seoul 03760, Republic of Korea.
authors.forename: Ahjeong
authors.identifier: http://orcid.org/0000-0001-6210-0769
authors.initials: A
authors.lastname: Son
keywords: NanoAptamer assay; bisphenol A; endocrine-disrupting chemicals; quantum dots; truncated aptamer