title: Molecular epidemiology of influenza A(H1N1)PDM09 hemagglutinin gene circulating in São Paulo State , Brazil: 2016 anticipated influenza season.
abstract: Compared to previous years, seasonal influenza activity commenced early in São Paulo State, Brazil, Southern hemisphere during the 2016 year. In order to investigate the genetic pattern of influenza A(H1N1)pdm09 in the State of Sao Paulo a total of 479 respiratory samples, collected in January by Sentinel Surveillance Units, were screened by real-time RT-PCR. A total of 6 Influenza viruses A(H1N1)pdm09 presenting ct values ≤ 30 were sequenced following phylogenetic analysis. The present study identified the circulation of the new 6B.1 subgroup (A/Sao Paulo/10-118/2016 and A/Sao Paulo/3032/2016). In addition, influenza A(H1N1)pdm09 group 6B has also been identified during January in the State of Sao Paulo. Despite amino acid changes and changes in potential glycosylation motifs, 6B.1 viruses were well inhibited by the reference ferret antiserum against A/California/07/2009 virus, the A(H1N1)pdm09 component of the vaccine for the 2016 influenza season.
authors.affiliation: Instituto Adolfo Lutz, Laboratório de Vírus Respiratórios, Núcleo de Doenças Respiratórias, CV/CCD/SES/SP, São Paulo, São Paulo, Brazil.
authors.forename: Katia Corrêa de Oliveira
authors.initials: KC
authors.lastname: Santos
authors.affiliation: Instituto Adolfo Lutz, Laboratório de Vírus Respiratórios, Núcleo de Doenças Respiratórias, CV/CCD/SES/SP, São Paulo, São Paulo, Brazil.
authors.forename: Daniela Bernardes Borges da
authors.initials: DB
authors.lastname: Silva
authors.affiliation: Instituto Adolfo Lutz, Laboratório de Vírus Respiratórios, Núcleo de Doenças Respiratórias, CV/CCD/SES/SP, São Paulo, São Paulo, Brazil.
authors.forename: Norio Augusto
authors.initials: NA
authors.lastname: Sasaki
authors.affiliation: Instituto Adolfo Lutz, Laboratório de Vírus Respiratórios, Núcleo de Doenças Respiratórias, CV/CCD/SES/SP, São Paulo, São Paulo, Brazil.
authors.forename: Margarete Aparecida
authors.initials: MA
authors.lastname: Benega
authors.affiliation: Centers for Disease Control and Prevention, Influenza Division, Atlanta, GA, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Garten
authors.affiliation: Instituto Adolfo Lutz, Laboratório de Vírus Respiratórios, Núcleo de Doenças Respiratórias, CV/CCD/SES/SP, São Paulo, São Paulo, Brazil.
authors.forename: Terezinha Maria de
authors.initials: TM
authors.lastname: Paiva
mesh_terms: D001938:Brazil / Q000453:epidemiology; D006388:Hemagglutinins; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D010802:Phylogeny; D012621:Seasons; D017422:Sequence Analysis, DNA
chemical_list: D006388:Hemagglutinins