title: Enzyme-mediated tagging of RNA.
abstract: RNA molecules can play diverse roles in the cell owing to their secondary structure dynamics and various binding modes. Studying localization and dynamics of RNA in vitro or in cells requires tagging with suitable reporter molecules-fluorophores being the most prominent ones. Enzymatic RNA labeling approaches are currently emerging as valuable alternatives to purely chemical synthesis and to binding- or hybridization-based RNA-imaging approaches. Different classes of enzymes allow for cotranscriptional or posttranscriptional installation of small functional groups in RNA. The enzymatic step is typically combined with a second chemical step, providing flexibility regarding the reporter. The flourishing field of bioorthogonal chemistry propels this approach. We will present latest achievements and remaining challenges in the field of enzyme-mediated RNA tagging and emphasize efforts to achieve site-specificity and intracellular labeling.
authors.affiliation: University of Münster, Department of Chemistry, Institute of Biochemistry, Wilhelm-Klemm-Str. 2, 48149 Münster, Germany.
authors.forename: Lea
authors.initials: L
authors.lastname: Anhäuser
authors.affiliation: University of Münster, Department of Chemistry, Institute of Biochemistry, Wilhelm-Klemm-Str. 2, 48149 Münster, Germany; Cells-in-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, Germany. Electronic address: a.rentmeister@uni-muenster.de.
authors.forename: Andrea
authors.initials: A
authors.lastname: Rentmeister
mesh_terms: D000818:Animals; D004798:Enzymes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis* / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes; D012313:RNA