title: Undec-10-enoic acid functionalized chitosan based novel nano-conjugate: An enhanced anti-bacterial/biofilm and anti-cancer potential.
abstract: Fatty acid functionalized chitosan conjugates are of great interest in cancer therapeutics because of its internalization through receptor mediated endocytosis into the cancer cells. Keeping the above fact into consideration, herein we synthesized the undec-10-enoic acid functionalized chitosan based undecyl-chitosan (U-CS) nano-bioconjugate with the use of DCC as a coupling agent. The U-CS conjugate synthesized was confirmed and characterized by FTIR,1H NMR, TGA, XRD, SEM and TEM analysis. Generally, it is well established that conjugates of oleic acid with human Alpha-lactalbumin (HAMLET) induce cytotoxicity in the altered cells, but not in healthy cells. To check our presumptions, anti-bacterial and anti-cancer potential of U-CS was evaluated against bacterial pathogens (Gram +ve and Gram -ve) and human cancer cell lines (HeLa, MDA-MB-231 and Hep3B). The results of our study clearly revealed that conjugate showed enhance anti-bacterial, anti-biofilm as well as anti-cancer efficacy as compared to pure and free form of the chitosan.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, 202002, India.
authors.forename: Khairujjaman
authors.initials: K
authors.lastname: Laskar
authors.affiliation: Molecular Immunology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Syed Mohd
authors.initials: SM
authors.lastname: Faisal
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, 202002, India. Electronic address: abduloafchem@gmail.com.
authors.forename: Abdul
authors.initials: A
authors.lastname: Rauf
authors.affiliation: Hybridoma Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Anees
authors.initials: A
authors.lastname: Ahmed
authors.affiliation: Molecular Immunology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Owais
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001419:Bacteria; D018441:Biofilms / Q000187:drug effects*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D007768:Lactalbumin; D058726:Nanoconjugates; D019301:Oleic Acid; D014476:Undecylenic Acids / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D058726:Nanoconjugates; D014476:Undecylenic Acids; D019301:Oleic Acid; D048271:Chitosan; D007768:Lactalbumin; C538763:undecylenic acid
keywords: Anti-bacterial; Anti-biofilm; Anti-cancer; Fatty acid; Nano-bioconjugate; Undecyl-chitosan