title: Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliateTetrahymena.
abstract: The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of ∼30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type or physiological condition. Here, we present evidence for compositionally distinct NPCs that form within a single cell in a binucleated ciliate. InTetrahymena thermophila, each cell contains both a transcriptionally active macronucleus (MAC) and a germline micronucleus (MIC). By combiningin silicoanalysis, mass spectrometry analysis for immuno-isolated proteins and subcellular localization analysis of GFP-fused proteins, we identified numerous novel components of MAC and MIC NPCs. Core members of the Nup107-Nup160 scaffold complex were enriched in MIC NPCs. Strikingly, two paralogs of Nup214 and of Nup153 localized exclusively to either the MAC or MIC NPCs. Furthermore, the transmembrane components Pom121 and Pom82 localize exclusively to MAC and MIC NPCs, respectively. Our results argue that functional nuclear dimorphism in ciliates is likely to depend on the compositional and structural specificity of NPCs.
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology (NICT), Kobe 651-2492, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Iwamoto
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology (NICT), Kobe 651-2492, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Osakada
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology (NICT), Kobe 651-2492, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Mori
authors.affiliation: Graduate School of Agriculture, Tohoku University, Osaki, Miyagi 989-6711, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: Graduate School of Life Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nagao
authors.affiliation: Graduate School of Life Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Chikashi
authors.initials: C
authors.lastname: Obuse
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology (NICT), Kobe 651-2492, Japan.|Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan.|Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan.
authors.forename: Yasushi
authors.identifier: 0000-0001-9407-8228
authors.initials: Y
authors.lastname: Hiraoka
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology (NICT), Kobe 651-2492, Japan tokuko@nict.go.jp.|Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan.|Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan.
authors.forename: Tokuko
authors.identifier: 0000-0002-3813-6785
authors.initials: T
authors.lastname: Haraguchi
mesh_terms: D017124:Conserved Sequence; D048648:Macronucleus / Q000378:metabolism; D048631:Micronucleus, Germline / Q000378:metabolism; D008954:Models, Biological; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D010539:Permeability; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D016808:Tetrahymena thermophila / Q000378:metabolism*
chemical_list: D028861:Nuclear Pore Complex Proteins; D015800:Protozoan Proteins
keywords: FG-Nup; Nuclear dimorphism; Nuclear envelope; Nucleoporin; Y-complex