title: SHPRH regulates rRNA transcription by recognizing the histone code in an mTOR-dependent manner.
abstract: Many DNA repair proteins have additional functions other than their roles in DNA repair. In addition to catalyzing PCNA polyubiquitylation in response to the stalling of DNA replication, SHPRH has the additional function of facilitating rRNA transcription by localizing to the ribosomal DNA (rDNA) promoter in the nucleoli. SHPRH was recruited to the rDNA promoter using its plant homeodomain (PHD), which interacts with histone H3 when the fourth lysine of H3 is not trimethylated. SHPRH enrichment at the rDNA promoter was inhibited by cell starvation, by treatment with actinomycin D or rapamycin, or by depletion of CHD4. SHPRH also physically interacted with the RNA polymerase I complex. Taken together, we provide evidence that SHPRH functions in rRNA transcription through its interaction with histone H3 in a mammalian target of rapamycin (mTOR)-dependent manner.
authors.affiliation: Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892.|Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, 689-798, Korea.
authors.forename: Deokjae
authors.initials: D
authors.lastname: Lee
authors.affiliation: Center for Genomic Integrity, Institute for Basic Science, Ulsan, 689-798, Korea.
authors.forename: Jungeun
authors.initials: J
authors.lastname: An
authors.affiliation: Center for Genomic Integrity, Institute for Basic Science, Ulsan, 689-798, Korea.
authors.forename: Young-Un
authors.initials: YU
authors.lastname: Park
authors.affiliation: Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Hungjiun
authors.initials: H
authors.lastname: Liaw
authors.affiliation: Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2371.
authors.forename: Roger
authors.initials: R
authors.lastname: Woodgate
authors.affiliation: Center for Genomic Integrity, Institute for Basic Science, Ulsan, 689-798, Korea.
authors.forename: Jun Hong
authors.initials: JH
authors.lastname: Park
authors.affiliation: Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892; kmyung@ibs.re.kr.|Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, 689-798, Korea.|Center for Genomic Integrity, Institute for Basic Science, Ulsan, 689-798, Korea.
authors.forename: Kyungjae
authors.initials: K
authors.lastname: Myung
mesh_terms: D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: C115323:CHD4 protein, human; D006657:Histones; D012335:RNA, Ribosomal; C516226:SHPRH protein, human; D044767:Ubiquitin-Protein Ligases; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D004265:DNA Helicases
keywords: SHPRH; histone H3 methylation; mTOR; rRNA transcription