title: Effects of sintering temperature on surface morphology/microstructure, in vitro degradability, mineralization and osteoblast response to magnesium phosphate as biomedical material.
abstract: Magnesium phosphate (MP) was fabricated using a chemical precipitation method, and the biological performances of MP sintered at different temperatures as a biomedical material was investigated. The results indicated that the densification and crystallinity of MP increased as the sintering temperature increased. As the sintering temperature increased, the degradability of MP in PBS decreased, and the mineralization ability in SBF significantly increased. In addition, the MP sintered at 800 °C (MP8) possessed the lowest degradability and highest mineralization ability. Moreover, the positive response of MG63 cells to MP significantly increased as the sintering temperature increased, and MP8 significantly promoted the cell spreading, proliferation, differentiation and expressions of osteogenic differentiation-related genes. Faster degradation of MP0 resulted in higher pH environments and ion concentrations, which led to negative responses to osteoblasts. However, the appropriate degradation of MP8 resulted in suitable pH environments and ion concentrations, which led to positive responses to osteoblasts. This study demonstrated that the sintering temperature substantially affected the surface morphology/microstructure, degradability and mineralization, and osteoblasts response to magnesium phosphate.
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Zhejiang Provincial Armed Police Corps Hospital, Hangzhou City, Zhejiang Province, 310051, P.R. China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Liehu
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Weizong
authors.initials: W
authors.lastname: Weng
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China. jiacansu@126.com.
authors.forename: Jiacan
authors.initials: J
authors.lastname: Su
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006358:Hot Temperature; D006801:Humans; D017616:Magnesium Compounds / Q000737:chemistry* / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010710:Phosphates / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D017616:Magnesium Compounds; D010710:Phosphates; C030781:magnesium phosphate