title: Immunochemical engineering of cell surfaces to generate virus resistance.
abstract: Modern immunochemical engineering allows the creation of cells that either secrete antibodies or incorporate them into various cellular compartments, including the plasma membrane. Because the receptors for most viruses are known, if one can achieve the proper stoichiometry and geometry, plasma membrane-associated antibodies to these receptors should block viral infection. In this report, we test this concept for two different viruses, human rhinovirus and HIV. Plasma membrane-tethered antibodies efficiently rendered cells permanently nonpermissive for infection by both these viruses. Membrane-bound antibodies were much more efficient than free antibody in preventing infection, likely because of the effective molarity of membrane bound antibodies. Such resistant cells may restore immune-competence to otherwise compromised HIV patients.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Jia
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Devin
authors.initials: D
authors.lastname: Sok
authors.affiliation: Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Wu
authors.affiliation: Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Zheng
authors.affiliation: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Burton
authors.affiliation: Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037; rlerner@scripps.edu.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D002462:Cell Membrane / Q000235:genetics / Q000276:immunology; D057809:HEK293 Cells; D015483:HIV Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000235:genetics / Q000276:immunology / Q000628:therapy; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019169:Jurkat Cells; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012229:Rhinovirus / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D015483:HIV Antibodies; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies
keywords: HIV; cell surfaces; prevention