title: Effects of Feedstock Sources on Inoculant Acclimatization: Start-up Strategies and Reactor Performance.
abstract: Different inoculum sources and acclimatization methods result in different substrate adaptation and biodegradability. To increase straw degradation rate, shorten the digester start-up time, and enhance the biogas production, we domesticated anaerobic sludge by adding microcrystalline cellulose (MCC). During acclimatization, the start-up strategies and reactor performance were investigated to analyze changes in feedstock adaption, biodegradability, and methanogen activity. The effect of the domesticated inoculum was evaluated by testing batch un-pretreated corn stover with a dewatered sludge (DS)-domesticated inoculum as a control. The results showed that (1) using MCC as a substrate rapidly improved microorganism biodegradability and adaptation. (2) MCC as domesticated substrate has relatively stable system and high mass conversion, but with low buffer capacity. (3) Macro- and micronutrients should be added for improving the activity of methanogenic and system's buffer capacity. (4) Using the domesticated inoculums and batch tests to anaerobically digest untreated corn stover yielded rapid biogas production of 292 mL, with an early peak value on the first day. The results indicated that cultivating directional inoculum can efficiently and quickly start-up digester. These investigated results to promote anaerobic digestion of straw for producing biogas speed up the transformation of achievements of biomass solid waste utilization have a positive promoting significance.
authors.affiliation: College of Environmental Science and Engineering, Nankai University, 94 Weijin Street, Tianjin, 300071, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: College of Environmental Science and Engineering, Nankai University, 94 Weijin Street, Tianjin, 300071, People's Republic of China.|College of Chemistry and Environmental Science, Kashi University, Xinjiang Kashi, 844006, People's Republic of China.
authors.forename: Liang-Huan
authors.initials: LH
authors.lastname: Wei
authors.affiliation: College of Environmental Science and Engineering, Nankai University, 94 Weijin Street, Tianjin, 300071, People's Republic of China.
authors.forename: Wei-Zun
authors.initials: WZ
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Nankai University, 94 Weijin Street, Tianjin, 300071, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Environmental Science and Engineering, Nankai University, 94 Weijin Street, Tianjin, 300071, People's Republic of China. jumeit@nankai.edu.cn.
authors.forename: Mei-Ting
authors.initials: MT
authors.lastname: Ju
mesh_terms: D000064:Acclimatization / Q000187:drug effects*; D000693:Anaerobiosis / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology*; D002482:Cellulose / Q000494:pharmacology*; D008697:Methane / Q000096:biosynthesis; D012722:Sewage / Q000382:microbiology
chemical_list: D056804:Biofuels; D012722:Sewage; D002482:Cellulose; D008697:Methane; C109691:microcrystalline cellulose
keywords: Acclimatization; Anaerobic digestion; Dewatered sludge; Inoculum; Microcrystalline cellulose