title: Determination of phthalates in bottled water by automated on-line solid phase extraction coupled to liquid chromatography with uv detection.
abstract: An on-line solid phase extraction coupled to liquid chromatography with UV detection (SPE/LC-UV) method was automated by the multisyringe flow-injection analysis (MSFIA) system for the determination of three phthalic acid esters (PAEs). The PAEs determined in drinking water stored in polyethylene terephthalate (PET) bottles of ten commercial brands were dimethyl phthalate (DMP), diethyl phthalate (DEP) and dibutyl phthalate (DBP). C18-bonded silica membrane was used for isolation and enrichment of the PAEs in water samples. The calibration range of the SPE/LC-UV method was 2.5-100μgL-1for DMP and DEP and 10-100μgL-1for DBP with correlation coefficients (r) ranging from 0.9970 to 0.9975. Limits of detection (LODs) were between 0.7 and 2.4μgL-1. Inter-day reproducibility performed at two concentration levels (10 and 100μgL-1) expressed as relative standard deviation (%RSD) were found in the range of 0.9-4.0%. The solvent volume was reduced to 18mL with a total analysis time of 48min per sample. The major species detected in bottled water samples was DBP reaching concentrations between 20.5 and 82.8μgL-1. The recovery percentages for the three analytes in drinking water were 80-115%. The migration test showed a great variation in the sum of migrated PAEs level (10.2-50.6μgL-1) among the PET bottle brands analyzed indicating that the presence of these contaminants in the plastic containers may depend on raw materials and the conditions used during their production process.
authors.affiliation: Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Salazar-Beltrán
authors.affiliation: Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Hinojosa-Reyes
authors.affiliation: Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Ruiz-Ruiz
authors.affiliation: Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico.
authors.forename: Aracely
authors.initials: A
authors.lastname: Hernández-Ramírez
authors.affiliation: Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66455, Mexico. Electronic address: jorge.guzmanmr@uanl.edu.mx.
authors.forename: Jorge
authors.initials: J
authors.lastname: Luis Guzmán-Mar
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D060766:Drinking Water / Q000032:analysis*; D006801:Humans; D010795:Phthalic Acids / Q000032:analysis* / Q000302:isolation & purification*; D010969:Plastics / Q000737:chemistry*; D052616:Solid Phase Extraction; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D060766:Drinking Water; D010795:Phthalic Acids; D010969:Plastics; D014874:Water Pollutants, Chemical; C032279:phthalic acid
keywords: Bottled water; LC-UV detection; MSFIA automation; Migration; On-line solid-phase extraction; PET containers; Phthalates