title: Pretreated quercetin protects gerbil hippocampal CA1 pyramidal neurons from transient cerebral ischemic injury by increasing the expression of antioxidant enzymes.
abstract: Quercetin (QE; 3,5,7,3',4'-pentahydroxyflavone), a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.
authors.affiliation: Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Bai Hui
authors.initials: BH
authors.lastname: Chen
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, South Korea.
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, South Korea.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea.
authors.forename: Jeong Hwi
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea.
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea.
authors.forename: Jae Chul
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea.
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.affiliation: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, South Korea.
authors.forename: Choong-Hyun
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.affiliation: Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, South Korea.
authors.forename: Jong-Dai
authors.initials: JD
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Hallym University, Chuncheon, South Korea.
authors.forename: Il Jun
authors.initials: IJ
authors.lastname: Kang
authors.affiliation: Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, South Korea.
authors.forename: Bich Na
authors.initials: BN
authors.lastname: Shin
authors.affiliation: Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, South Korea.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, South Korea.
authors.forename: Yun Lyul
authors.identifier: 0000-0003-0504-5825
authors.initials: YL
authors.lastname: Lee
authors.affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea.
authors.forename: Seung Min
authors.identifier: 0000-0002-3594-8403
authors.initials: SM
authors.lastname: Park
keywords: Cu/Zn superoxide dismutase; Mn superoxide dismutase; catalase; flavonoids; glutathione peroxidase; nerve regeneration; neural regeneration; transient cerebral ischemia