title: Ellagic acid protects against neuron damage in ischemic stroke through regulating the ratio of Bcl-2/Bax expression.
abstract: An oxygen-glucose deprivation and reoxygenation model in primary cultured rat cortical neurons was developed for this study to investigate the effects of ellagic acid (EA), a low-molecular-weight polyphenol, on neuron cells and their function, and to evaluate whether EA can be safely utilized by humans as a functional food or therapeutic agent. Administration of EA significantly decreased the volume of cerebrum infarction and the neurological deficit scores of the rats; EA treatment also increased the number of Bcl-2-positive cells and the ratio of Bcl-2-positive to Bax-positive neurons in the semidarkness zone near the brain ischemic focus in the photothrombotic cerebral ischemia model. Treatment of EA resulted in increased neuron viability, cell nuclear integrity, and the ratio of Bcl-2/Bax expression in the primary cultured neuron model; EA treatment also lead to a decrease in the number of apoptotic cells. Our results therefore suggest a specific mechanism for the beneficial effects of EA, providing new insights into how it provides neuroprotection. To the best of our knowledge, these results represent new insights on the mechanisms of the brain cell protective activity of EA. Thus, EA may be used in functional foods or medicines to help treat nerve dysfunction, neurodegenerative disease, and aging.
authors.affiliation: a Key Lab of Ministry of Education, National Research Center on Minority Medicine and Translational Neuroscience, Minzu University of China, Beijing 100081, People's Republic of China.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Liu
authors.affiliation: a Key Lab of Ministry of Education, National Research Center on Minority Medicine and Translational Neuroscience, Minzu University of China, Beijing 100081, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Deng
authors.affiliation: a Key Lab of Ministry of Education, National Research Center on Minority Medicine and Translational Neuroscience, Minzu University of China, Beijing 100081, People's Republic of China.
authors.forename: Shuran
authors.initials: S
authors.lastname: Li
authors.affiliation: a Key Lab of Ministry of Education, National Research Center on Minority Medicine and Translational Neuroscience, Minzu University of China, Beijing 100081, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: a Key Lab of Ministry of Education, National Research Center on Minority Medicine and Translational Neuroscience, Minzu University of China, Beijing 100081, People's Republic of China.
authors.forename: Keqin
authors.initials: K
authors.lastname: Li
authors.affiliation: a Key Lab of Ministry of Education, National Research Center on Minority Medicine and Translational Neuroscience, Minzu University of China, Beijing 100081, People's Republic of China.
authors.forename: Gulibanumu
authors.initials: G
authors.lastname: Kebaituli
authors.affiliation: b Experimental Research Center, China Academy of traditional Chinese Medicine, Beijing, 100700, People's Republic of China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Li
authors.affiliation: c State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004610:Ellagic Acid / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000188:drug therapy*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; C000617196:Bcl2 protein, rat; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D004610:Ellagic Acid
keywords: AVC; Bax; Bcl-2; OGD/R; acide ellagique; ellagic acid; mort de cellule nerveuse; nerve cell death; neuron; neurone; stroke