title: CalR is required for the expression of T6SS2 and the adhesion of Vibrio parahaemolyticus to HeLa cells.
abstract: Vibrio parahaemolyticus expresses one major virulence determinant T6SS2, which is constituted into three putative operons, i.e., VPA1027-1024, VPA1043-1028, and VPA1044-1046. CalR, a LysR-type transcriptional regulator, was originally identified as a repressor of the swarming motility and T3SS1 gene expression. As shown in this study, CalR binds to the promoter-proximal region of each of the three operons to activate their transcription, and moreover, CalR activates the adhesion of V. parahaemolyticus to HeLa cells. In addition, competitive EMSAs demonstrated that CalR acts as an antagonist of H-NS in V. parahaemolyticus. Collectively, these studies confirmed a new physiological role for CalR in V. parahaemolyticus.
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: George
authors.initials: G
authors.lastname: Osei-Adjei
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, 102206, China.
authors.forename: He
authors.initials: H
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100000, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100000, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China. huxinx@ujs.edu.cn.
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100000, China. yhy324@aliyun.com.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China. zhangyiquanq@163.com.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D009876:Operon; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000069376:Type VI Secretion Systems / Q000235:genetics / Q000378:metabolism*; D014735:Vibrio Infections / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors; D000069376:Type VI Secretion Systems
keywords: CalR; T6SS2; Vibrio parahaemolyticus