title: [O-GlcNAc glycosylation influences the biological behaviors and etoposide-induced apoptosis of Nalm-6 cells].
abstract: Objective:To explore the effects of O-GlcNAc glycosylation and its key enzyme OGT on the biological behaviors and etoposide (Vp16) -induced apoptosis of Nalm-6 cells.Methods:Low O-GlcNAc modified Nalm-6 cells model was established with Alloxan, an inhibitor of OGT. The influence of Alloxan on Nalm-6 cells proliferation was checked by CCK-8 assay, apoptosis and cell cycle by flow cytometry. Nalm-6 cells were treated with different concentrations of Vp16 for 12 h, and then the O-GlcNAc level and the expressions of OGT were examined by Western blot. After treating Nalm-6 with Alloxan for 24 h and then 5 μg/ml of Vp16 for 12 h, the apoptosis of different groups were measured with flow cytometry, and the expression of apoptosis-associated proteins Bax and Bcl-2 were examined by Western blot.Results:With the concentration of Vp16 increasing, the O-GlcNAc modified levels of total protein and the expression of OGT were up regulated (P<0.05,n=6) ; Alloxan could slow down the proliferation capacity, induce apoptosis[ (15.190±2.539) %vs(21.910±4.105) %,P=0.007], arrest cell cycle[G1 phase: (43.534±4.453) %vs(57.322±6.091) %,P=0.003; S phase: (50.747±5.937) %vs(37.201±4.661) %,P=0.001]. Alloxan could inhibit the apoptosis caused by Vp16[ (75.195±13.845) %vs(52.741±10.815) %,P=0.011]along with Bax decreasing (5.496±1.998vs2.950±0.703,P=0.015) and Bcl-2 increasing (0.454±0.125vs0.803±0.223,P=0.013) .Conclusion:Changes of O-GlcNAc modified level of Nalm-6 cells along with the inhibition of OGT could influence the biological behaviors and inhibit apoptosis induced by Vp16.
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: X L
authors.initials: XL
authors.lastname: Ju
mesh_terms: D000496:Alloxan; D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D005047:Etoposide; D006031:Glycosylation; D015854:Up-Regulation
chemical_list: D005047:Etoposide; D000496:Alloxan
keywords: Etoposide; Glycosylation; Nalm-6 cells; O-linked N-acetylglucosamine; β-N-acetylglucosaminyltransferase