title: Detection of l-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates.
abstract: Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting l-alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.
authors.affiliation: Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Cellier
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Arthur L
authors.initials: AL
authors.lastname: James
authors.affiliation: Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Orenga
authors.affiliation: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Perry
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Ari K
authors.initials: AK
authors.lastname: Rasul
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK. Electronic address: steven.stanforth@northumbria.ac.uk.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Stanforth
mesh_terms: D000880:Anthraquinones / Q000737:chemistry* / Q000378:metabolism; D018826:CD13 Antigens / Q000378:metabolism*; D002863:Chromogenic Compounds / Q000737:chemistry* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000201:enzymology / Q000254:growth & development; D008670:Metals / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D000880:Anthraquinones; D002863:Chromogenic Compounds; D008670:Metals; C010078:alizarin; D018826:CD13 Antigens
keywords: Chromogenic enzyme substrates; Microorganisms; l-Alanylaminopeptidase detection