title: Differential effects of high and low glucose concentrations during lipolysis-like conditions on bovine in vitro oocyte quality, metabolism and subsequent embryo development.
abstract: Lipolytic metabolic conditions are traditionally associated with elevated non-esterified fatty acid (NEFA) concentrations, but may also be accompanied by hyperglycaemia in obesity or by hypoglycaemia during a negative energy balance status. Elevated NEFA concentrations disrupt oocyte and embryo development and quality, but little is known about whether the effects of lipolytic conditions on oocyte developmental competence are modulated by glucose availability. To answer this, bovine cumulus-oocyte complexes (COCs) were matured under different conditions: physiological NEFA (72µM) and normal glucose (5.5mM), pathophysiologically high NEFA (420µM) and normal glucose, high NEFA and high glucose (9.9mM), high NEFA and low glucose (2.8mM). Developmental potential, cumulus expansion and metabolism of COCs exposed to high NEFA and low glucose were affected to a greater extent compared with COCs matured under high NEFA and high glucose conditions. High NEFA and high glucose conditions caused a moderate increase in oocyte reactive oxygen species compared with their high NEFA and low glucose or control counterparts. Blastocyst metabolism and the transcriptome of metabolic and oxidative stress-related genes were not affected. However, both lipolytic conditions associated with hyper- or hypoglycaemia led to surviving embryos of reduced quality with regards to apoptosis and blastomere allocation.
authors.affiliation: Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: De Bie
authors.affiliation: Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
authors.forename: W F A
authors.initials: WFA
authors.lastname: Marei
authors.affiliation: Departamento de Reproduccion Animal, Instituto nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Crta. De la Coruña, km 7,5 28040 Madrid, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Maillo
authors.affiliation: Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Jordaens
authors.affiliation: Departamento de Reproduccion Animal, Instituto nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Crta. De la Coruña, km 7,5 28040 Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gutierrez-Adan
authors.affiliation: Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
authors.forename: P E J
authors.initials: PEJ
authors.lastname: Bols
authors.affiliation: Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
authors.forename: J L M R
authors.initials: JLMR
authors.lastname: Leroy
mesh_terms: D000818:Animals; D002417:Cattle; D054885:Cumulus Cells / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D005947:Glucose / Q000008:administration & dosage*; D059471:In Vitro Oocyte Maturation Techniques; D008066:Lipolysis / Q000187:drug effects* / Q000502:physiology; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D005947:Glucose