title: Gelatin device for the delivery of growth factors involved in endochondral ossification.
abstract: Controlled release drug delivery systems are well established as oral and implantable dosage forms. However, the controlled release paradigm can also be used to present complex soluble signals responsible for cellular organization during development. Endochondral ossification (EO), the developmental process of bone formation from a cartilage matrix is controlled by several soluble signals with distinct functions that vary in structure, molecular weight and stability. This makes delivering them from a single vehicle rather challenging. Herein, a gelatin-based delivery system suitable for the delivery of small molecules as well as recombinant human (rh) proteins (rhWNT3A, rhFGF2, rhVEGF, rhBMP4) is reported. The release behavior and biological activity of the released molecules was validated using analytical and biological assays, including cell reporter systems. The simplicity of fabrication of the gelatin device should foster its adaptation by the diverse scientific community interested in interrogating developmental processes, in vivo.
authors.affiliation: Institute for Macromolecular Chemistry, Hermann Staudinger Haus, University of Freiburg, Freiburg, Germany.|BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
authors.forename: Lucas A J
authors.initials: LA
authors.lastname: Ahrens
authors.affiliation: Institute for Macromolecular Chemistry, Hermann Staudinger Haus, University of Freiburg, Freiburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vonwil
authors.affiliation: Institute for Macromolecular Chemistry, Hermann Staudinger Haus, University of Freiburg, Freiburg, Germany.|BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
authors.forename: Jon
authors.initials: J
authors.lastname: Christensen
authors.affiliation: Institute for Macromolecular Chemistry, Hermann Staudinger Haus, University of Freiburg, Freiburg, Germany.|BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
authors.forename: V Prasad
authors.identifier: http://orcid.org/0000-0001-5125-9678
authors.initials: VP
authors.lastname: Shastri
mesh_terms: D055415:Bone Morphogenetic Protein 4 / Q000008:administration & dosage* / Q000494:pharmacology; D002460:Cell Line; D016503:Drug Delivery Systems / Q000379:methods*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000494:pharmacology; D005780:Gelatin / Q000627:therapeutic use*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage* / Q000494:pharmacology; D060509:Wnt3A Protein / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: C525809:BMP4 protein, human; D055415:Bone Morphogenetic Protein 4; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C555510:WNT3A protein, human; D060509:Wnt3A Protein; D016222:Fibroblast Growth Factor 2; D005780:Gelatin