title: Clearing the outer mitochondrial membrane from harmful proteins via lipid droplets.
abstract: In recent years it turned out that there is not only extensive communication between the nucleus and mitochondria but also between mitochondria and lipid droplets (LDs) as well. We were able to demonstrate that a number of proteins shuttle between LDs and mitochondria and it depends on the metabolic state of the cell on which organelle these proteins are predominantly localized. Responsible for the localization of the particular proteins is a protein domain consisting of twoα-helices, which we termed V-domain according to the predicted structure. So far we have detected this domain in the following proteins: mammalian BAX, BCL-XL, TCTP and yeast Mmi1p and Erg6p. According to our experiments there are two functions of this domain: (1) shuttling of proteins to mitochondria in times of stress and apoptosis; (2) clearing the outer mitochondrial membrane from pro- as well as anti-apoptotic proteins by moving them to LDs after the stress ceases. In this way the LDs are used by the cell to modulate stress response.
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Johannes
authors.identifier: 0000-0001-5309-1936
authors.initials: J
authors.lastname: Bischof
authors.affiliation: Institute of Physiology, Centre of Physiology and Pharmacology, Medical University of Vienna , Vienna, Austria.
authors.forename: Manuel
authors.initials: M
authors.lastname: Salzmann
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Maria Karolin
authors.initials: MK
authors.lastname: Streubel
authors.affiliation: Laboratory of Cell Reproduction, Institute of Microbiology of AS CR, v.v.i. , Prague, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Hasek
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Geltinger
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Jutta
authors.initials: J
authors.lastname: Duschl
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Bresgen
authors.affiliation: Department of Molecular Biology, University of Salzburg , Salzburg, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Briza
authors.affiliation: Laboratory of Cell Reproduction, Institute of Microbiology of AS CR, v.v.i. , Prague, Czech Republic.
authors.forename: Danusa
authors.initials: D
authors.lastname: Haskova
authors.affiliation: Laboratory of Cell Reproduction, Institute of Microbiology of AS CR, v.v.i. , Prague, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Lejskova
authors.affiliation: Faculty of Medicine, University of Prishtina , Prishtinë, Kosova.
authors.forename: Mentor
authors.initials: M
authors.lastname: Sopjani
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Richter
authors.affiliation: Department of Cell Biology and Physiology, Division of Genetics, University of Salzburg , Salzburg, Austria.
authors.forename: Mark
authors.initials: M
authors.lastname: Rinnerthaler