title: Bacterial communities on facial skin of teenage and elderly Thai females.
abstract: The Human Microbiome Project was first established to understand the roles of human-associated microbes to human health and disease. This study presents preliminary findings of Thai female facial skin microbiome using three pooled samples from groups of skin microbiome profiles, namely (1) healthy and (2) acne-prone young adults (teenage.hea and teenage.acn) and (3) healthy elderly adults (elderly.hea) based on standard dermatological criteria. These samples were sequenced using 454-pyrosequencing targeting 16S rRNA (V3-V4 regions). Good's coverage index of greater than 92% shows sufficient sampling of our data for each group. Three unique OTUs for each microbiome profile (43, 258 and 59 for teenage.hea, teenage.acn and ederly.hea, respectively) were obtained with 134 shared OTUs among the three datasets. Based on Morisita-Horn similarity coefficient, age is the major factor that brings the community relationship factor closer. The comparison among the three datasets reveal majority of Gemmatimonadetes, Planctomycetes and Nitrospirae in the teenage.hea, whereas Firmicutes are more prevalent in teenage.acn and elderly.hea skin types. In addition, when comparing Thai facial microbial diversity with the 16S data from U.S. forehead female database, significant differences were found among orders of bacteria, pointing to possible differences in human ecto-flora.
authors.affiliation: Department of Microbiology, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok, 10330, Thailand. naraporn.s@chula.ac.th.|Omics Sciences and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. naraporn.s@chula.ac.th.
authors.forename: Naraporn
authors.identifier: http://orcid.org/0000-0002-7830-3509
authors.initials: N
authors.lastname: Somboonna
authors.affiliation: Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
authors.forename: Alisa
authors.initials: A
authors.lastname: Wilantho
authors.affiliation: Division of Dermatology, Department of Medicine, Phramongkutklao Hospital, Bangkok, 10400, Thailand.
authors.forename: Chutika
authors.initials: C
authors.lastname: Srisuttiyakorn
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Anunchai
authors.initials: A
authors.lastname: Assawamakin
authors.affiliation: Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand. sissades@biotec.or.th.
authors.forename: Sissades
authors.initials: S
authors.lastname: Tongsima
mesh_terms: D000152:Acne Vulgaris / Q000382:microbiology*; D000328:Adult; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D005260:Female; D006801:Humans; D064307:Microbiota / Q000235:genetics*; D008875:Middle Aged; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012867:Skin / Q000382:microbiology*; D013785:Thailand; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Bacteria; Human microbiome; Microbial diversity; Pyrosequencing; Skin