title: Electrochemical and optical study of metallothionein interactions with prion proteins.
abstract: The prion protein (PrPC) can be structurally shifted to its PrPScisoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPCand MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrPC, MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS). Electrochemical properties of PrP itself and its interactions with both the Cu(II) ions and MTs have been found. Based on the results obtained, we hypothesised the formation of the complex in molar ratio 2:1 (PrPC:MT). Surface plasmon resonance imaging (SPRi) was used as a control reference assay to further confirm results obtained by the electrochemical approach, such as the specific interactions between PrPCand MT-3.
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Department of Veterinary Medicine, University of Cambridge, UK.
authors.forename: Alzbeta
authors.initials: A
authors.lastname: Cardova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Adam
authors.affiliation: Dipartimento di Chimica "Ugo Schiff" and CSGI, Università di Firenze, Sesto Fiorentino, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mariani
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Richtera
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Heger
authors.affiliation: Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
authors.forename: Jan
authors.initials: J
authors.lastname: Labuda
authors.affiliation: Dipartimento di Chimica "Ugo Schiff" and CSGI, Università di Firenze, Sesto Fiorentino, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Minunni
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic. Electronic address: vojtech.adam@mendelu.cz.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D003300:Copper; D055664:Electrochemical Techniques; D008668:Metallothionein; D000072002:Prion Proteins
chemical_list: D000072002:Prion Proteins; D003300:Copper; D008668:Metallothionein
keywords: Brain; Electrochemistry; MT; Neurodegenerative disorders; PrP