title: Lysozyme Associated Liposomal Gentamicin Inhibits Bacterial Biofilm.
abstract: Bacteria on living or inert surfaces usually form biofilms which make them highly resistant to antibiotics and immune clearance. Herein, we develop a simple approach to overcome the above conundrum through lysozyme-associated liposomal gentamicin (LLG). The association of lysozyme to the surface of liposomes can effectively reduce the fusion of liposomes and undesirable payload release in regular storage or physiological environments. The LLG was more effective at damaging established biofilms and inhibiting biofilm formation of pathogens including Gram-positive and Gram-negative bacteria than gentamicin alone. This strategy may provide a novel approach to treat infections due to bacterial biofilm.
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. stellanox1019@163.com.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. wzhj10111@nwafu.edu.cn.
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. 15291850570@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. baihu@nwafu.edu.cn.
authors.forename: Hu
authors.initials: H
authors.lastname: Bai
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. sunyuelin_123@nwafu.edu.cn.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. jduan@nwafu.edu.cn.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Duan
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. mhb1025@nwafu.edu.cn.
authors.forename: Haibo
authors.initials: H
authors.lastname: Mu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D008081:Liposomes; D009113:Muramidase
keywords: biofilm; gentamicin; liposome; lysozyme