title: Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production.
abstract: Komagataella phaffii (formerly Pichia pastoris) is a well-known fungal system for heterologous protein production in the context of modern biotechnology. To obtain higher protein titers in this system many researchers have sought to optimize gene expression by increasing the levels of transcription of the heterologous gene. This has been typically achieved by manipulating promoter sequences or by generating clones bearing multiple copies of the desired gene. The aim of this work is to describe how these different molecular strategies have been applied in K. phaffii presenting their advantages and drawbacks.
authors.affiliation: a Laboratório de Biologia Molecular, Instituto de Ciências Biológicas , Universidade de Brasília , Brasília , DF , Brazil.
authors.forename: Luiza Cesca
authors.initials: LC
authors.lastname: Piva
authors.affiliation: a Laboratório de Biologia Molecular, Instituto de Ciências Biológicas , Universidade de Brasília , Brasília , DF , Brazil.
authors.forename: Maritza Ocampo
authors.initials: MO
authors.lastname: Bentacur
authors.affiliation: a Laboratório de Biologia Molecular, Instituto de Ciências Biológicas , Universidade de Brasília , Brasília , DF , Brazil.
authors.forename: Viviane Castelo Branco
authors.initials: VCB
authors.lastname: Reis
authors.affiliation: a Laboratório de Biologia Molecular, Instituto de Ciências Biológicas , Universidade de Brasília , Brasília , DF , Brazil.
authors.forename: Janice Lisboa
authors.initials: JL
authors.lastname: De Marco
authors.affiliation: a Laboratório de Biologia Molecular, Instituto de Ciências Biológicas , Universidade de Brasília , Brasília , DF , Brazil.
authors.forename: Lidia Maria Pepe de
authors.initials: LMP
authors.lastname: Moraes
authors.affiliation: a Laboratório de Biologia Molecular, Instituto de Ciências Biológicas , Universidade de Brasília , Brasília , DF , Brazil.
authors.forename: Fernando Araripe Gonçalves
authors.initials: FAG
authors.lastname: Torres
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D018628:Gene Dosage / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D024861:Genetic Enhancement / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors
keywords: Komagataella phaffii; gene transcription; heterologous expression; multi-copy integration; promoter engineering