title: Templated Self-Assembly of a Covalent Polymer Network for Intracellular Protein Delivery and Traceless Release.
abstract: Trafficking proteins inside cells is an emerging field with potential utility in basic cell biology and biological therapeutics. A robust and sustainable delivery strategy demands not only good protection of the cargo but also reversibility in conjugation and activity. We report a protein-templated polymer self-assembly strategy for forming a sheath around the proteins and then tracelessly releasing them in the cytosol. The versatility of the approach, demonstrated here, suggests that the strategy is compatible with a wide array of biologics.
authors.affiliation: Department of Chemistry, ‡Department of Polymer Science and Engineering, §The Center for Bioactive Delivery, Institute for Applied Life Sciences, and ∥Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003-9336, United States.
authors.forename: Kingshuk
authors.initials: K
authors.lastname: Dutta
authors.affiliation: Department of Chemistry, ‡Department of Polymer Science and Engineering, §The Center for Bioactive Delivery, Institute for Applied Life Sciences, and ∥Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003-9336, United States.
authors.forename: Ding
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Chemistry, ‡Department of Polymer Science and Engineering, §The Center for Bioactive Delivery, Institute for Applied Life Sciences, and ∥Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003-9336, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, ‡Department of Polymer Science and Engineering, §The Center for Bioactive Delivery, Institute for Applied Life Sciences, and ∥Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003-9336, United States.
authors.forename: Alexander E
authors.identifier: 0000-0002-9924-3429
authors.initials: AE
authors.lastname: Ribbe
authors.affiliation: Department of Chemistry, ‡Department of Polymer Science and Engineering, §The Center for Bioactive Delivery, Institute for Applied Life Sciences, and ∥Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003-9336, United States.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Department of Chemistry, ‡Department of Polymer Science and Engineering, §The Center for Bioactive Delivery, Institute for Applied Life Sciences, and ∥Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003-9336, United States.
authors.forename: S
authors.identifier: 0000-0002-6475-6726
authors.initials: S
authors.lastname: Thayumanavan
mesh_terms: D003600:Cytosol / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D011108:Polymers; D011506:Proteins