title: Promoting Wound Healing Using Low Molecular Weight Fucoidan in a Full-Thickness Dermal Excision Rat Model.
abstract: Low molecular weight fucoidan (LMF) has been reported to possess anti-inflammatory and antioxidant activities. Thus, we examined the effects of LMF extracted fromUndaria pinnatifidaon dermal wounds. Five round dermal wounds were created on the dorsal back of rats, and they were then treated topically with distilled water (DW), Madecasol Care™ (MC) or LMF at 200, 100 and 50 mg/mL, twice a day for a week. There were dose-dependent increases in wound contraction in the groups receiving LMF but not in the MC group, compared with the DW. Histopathological examination revealed that LMF treatment accelerated wound healing, which was supported by increases in granular tissue formation on day four post-treatment but a decrease on day seven, accompanied by an evident reduction in inflammatory cells. In the LMF-treated wounds, collagen distribution and angiogenesis were increased in the granular tissue on days four and seven post-treatment. Immunoreactive cells for transforming growth factor-β1, vascular endothelial growth factor receptor-2 or matrix metalloproteinases 9 were also increased, probably due to tissue remodeling. Furthermore, LMF treatment reduced lipid peroxidation and increased antioxidant activities. These suggested that LMF promotes dermal wound healing via complex and coordinated antioxidant, anti-inflammatory and growth factor-dependent activities.
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. kjadjh123@naver.com.
authors.forename: Jun-Hyeong
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. ck0190@hanmail.net.
authors.forename: Seong-Hun
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. sjp124@gmail.com.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Preventive Medicine, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. gksxntk@dhu.ac.kr.
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Pathology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. moguri@dhu.ac.kr.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Urology, College of Medicine, Yeungnam University, Daegu 42415, Korea. sph04@yu.ac.kr.
authors.forename: Phil Hyun
authors.initials: PH
authors.lastname: Song
authors.affiliation: Faculty of Physical Education, College of Physical Education, Keimyung University, Daegu 42601, Korea. cmcho12@kmu.ac.kr.
authors.forename: Chang-Mo
authors.initials: CM
authors.lastname: Cho
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. gucci200@hanmail.net.
authors.forename: Sae-Kwang
authors.initials: SK
authors.lastname: Ku
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. dvmsong@hotmail.com.
authors.forename: Chang-Hyun
authors.initials: CH
authors.lastname: Song
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008970:Molecular Weight; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D044648:Undaria / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D003094:Collagen; C007789:fucoidan
keywords: anti-inflammation; antioxidant; fucoidan; low molecular weight; skin; wound