title: Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke.
abstract: Stroke survivors are typically left with structural brain damage and associated functional impairment in the chronic phase of injury, for which few therapeutic options exist. We reported previously that transplantation of human embryonic stem cell (hESC)-derived neural stem cells together with Matrigel scaffolding into the brains of rats after focal ischemia reduced infarct volume and improved neurobehavioral performance. Matrigel is a gelatinous protein mixture extracted from mouse sarcoma cells, thus would not be approved for use as a scaffold clinically. In this study, we generated a gel-like scaffold from plasma that was controlled by changing the concentration of CaCl2.In vitrostudy confirmed that 10-20 mM CaCl2and 10-40% plasma did not affect the viability and proliferation of human and rat bone marrow mesenchymal stem/stromal cells (BMSCs) and neural stem cells (NSCs). We transplanted plasma scaffold in combination of BMSCs into the cystic cavity after focal cerebral ischemia, and found that the atrophy volume was dramatically reduced and motor function was significantly improved in the group transplanted with scaffold/BMSCs compared with the groups treated with vehicle, scaffold or BMSCs only. Our data suggest that plasma-derived scaffold in combination of BMSCs is feasible for tissue engineering approach for the stroke treatment.
authors.affiliation: 1Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China; 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Fen
authors.initials: F
authors.lastname: Sun
authors.affiliation: 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA; 3Department of Rehabilitation, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Jixian
authors.initials: J
authors.lastname: Wang
authors.affiliation: 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Luokun
authors.initials: L
authors.lastname: Xie
authors.affiliation: 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Chenqi
authors.initials: C
authors.lastname: Yang
authors.affiliation: 1Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China; 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Mengxiong
authors.initials: M
authors.lastname: Pan
authors.affiliation: 1Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Shao
authors.affiliation: 4Med-x Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Yang
authors.affiliation: 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Yang
authors.affiliation: 1Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors.forename: Qichuan
authors.initials: Q
authors.lastname: ZhuGe
authors.affiliation: 1Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China; 2Institute for Healthy Aging, University of North Texas Health Science Center at Fort Worth, TX 76107, USA.
authors.forename: Kunlin
authors.initials: K
authors.lastname: Jin
keywords: aging; aging-related diseases; diagnosis; frailty; regulation; senescence