title: Linoleic acid isomerase gene FgLAI12 affects sensitivity to salicylic acid, mycelial growth and virulence of Fusarium graminearum.
abstract: Fusarium graminearum is the major causal agent of fusarium head blight in wheat, a serious disease worldwide. Linoleic acid isomerase (LAI) catalyses the transformation of linoleic acid (LA) to conjugated linoleic acid (CLA), which is beneficial for human health. We characterised a cis-12 LAI gene of F. graminearum (FGSG_02668; FgLAI12), which was downregulated by salicylic acid (SA), a plant defence hormone. Disruption of FgLAI12 in F. graminearum resulted in decreased accumulation of cis-9,trans-11 CLA, enhanced sensitivity to SA, and increased accumulation of LA and SA in wheat spikes during infection. In addition, mycelial growth, accumulation of deoxynivalenol, and pathogenicity in wheat spikes were reduced. Re-introduction of a functional FgLAI12 gene into ΔFgLAI12 recovered the wild-type phenotype. Fluorescent microscopic analysis showed that FgLAI12 protein was usually expressed in the septa zone of conidia and the vacuole of hyphae, but was expressed in the cell membrane of hyphae in response to exogenous LA, which may be an element of LA metabolism during infection by F. graminearum. The cis-12 LAI enzyme encoded by FgLAI12 is critical for fungal response to SA, mycelial growth and virulence in wheat. The gene FgLAI12 is potentially valuable for biotechnological synthesis of cis-9,trans-11 CLA.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Ya-Zhou
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Wei
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Bin-Jie
authors.initials: BJ
authors.lastname: Xu
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Zhen-Ru
authors.initials: ZR
authors.lastname: Guo
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Cao
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Han
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Feng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Qiao
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Lu-Juan
authors.initials: LJ
authors.lastname: Zong
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zheng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Deng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Qian-Tao
authors.initials: QT
authors.lastname: Jiang
authors.affiliation: Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wei
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Qi
mesh_terms: D055162:Biocatalysis / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity*; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007535:Isomerases / Q000235:genetics* / Q000378:metabolism; D007536:Isomerism; D019787:Linoleic Acid / Q000737:chemistry / Q000378:metabolism*; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development*; D010935:Plant Diseases / Q000382:microbiology; D020156:Salicylic Acid / Q000494:pharmacology*; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D013347:Subcellular Fractions / Q000378:metabolism; D014908:Triticum / Q000382:microbiology; D014774:Virulence / Q000187:drug effects / Q000235:genetics
chemical_list: D019787:Linoleic Acid; D007535:Isomerases; D020156:Salicylic Acid