title: Control of tissue size and development by a regulatory element in theyorkie3'UTR.
abstract: Regulation of the Hippo pathway via phosphorylation of Yorkie (Yki), theDrosophilahomolog of human Yes-associated protein 1, is conserved fromDrosophilato humans. Overexpression of a non-phosphorylatable form of Yki induces severe overgrowth in adult fly eyes. Here, we show thatykimRNA associates with microsomal fractions and forms foci that partially colocalize to processing bodies in the vicinity of endoplasmic reticulum. This localization is dependent on a stem-loop (SL) structure in the 3' untranslated region ofyki. Surprisingly, expression of SL deletedykiin eye imaginal discs also results in severe overgrowth phenotypes. When the structure of the SL is disrupted, Yki protein levels increase without a significant effect on RNA levels. When the SL is completely removed, protein levels drastically increase, but in this case, due to increased RNA stability. In the latter case, we show that the increased RNA accumulation is due to removal of a putativemiR-8seed sequence in the SL. These data demonstrate the function of two novel regulatory mechanisms, both controlled by theykiSL element, that are essential for proper Hippo pathway mediated growth regulation.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Takanari
authors.initials: T
authors.lastname: Umegawachi
authors.affiliation: Department of Applied Biology, Kyoto Institute of TechnologyMatsugasaki, Sakyo-ku, Kyoto 606-8585, Japan; The Center for Advanced Insect Research Promotion, Kyoto Institute of TechnologyMatsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Koshida
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Medical Institute of Bioregulation, Kyushu University Fukuoka 812-8582, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
authors.affiliation: Medical Institute of Bioregulation, Kyushu University Fukuoka 812-8582, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sato
authors.affiliation: Medical Institute of Bioregulation, Kyushu University Fukuoka 812-8582, Japan.
authors.forename: Mikita
authors.initials: M
authors.lastname: Suyama
authors.affiliation: Banting and Best Department of Medical Research, University of TorontoToronto, Ontario M5G 1L6, Canada; Department of Molecular Genetics, University of TorontoToronto, Ontario M5G 1L6, Canada; Donnelly Centre, University of TorontoToronto, Ontario M5G 1L6, Canada.
authors.forename: Henry M
authors.initials: HM
authors.lastname: Krause
authors.affiliation: Department of Applied Biology, Kyoto Institute of TechnologyMatsugasaki, Sakyo-ku, Kyoto 606-8585, Japan; The Center for Advanced Insect Research Promotion, Kyoto Institute of TechnologyMatsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
keywords: hippo pathway; mRNA localization; yki mRNA