title: Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension.
abstract: We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only during pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.
authors.affiliation: Genetic Laboratory Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia.
authors.forename: Mina
authors.initials: M
authors.lastname: Toljic
authors.affiliation: High-Risk Pregnancy Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia; School of Medicine, University of Belgrade, Street Dr Subotica 8, 11000 Belgrade, Serbia.
authors.forename: Amira
authors.initials: A
authors.lastname: Egic
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Street Vojvode Stepe 450, 11000 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Munjas
authors.affiliation: High-Risk Pregnancy Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia; School of Medicine, University of Belgrade, Street Dr Subotica 8, 11000 Belgrade, Serbia.
authors.forename: Natasa
authors.initials: N
authors.lastname: Karadzov Orlic
authors.affiliation: High-Risk Pregnancy Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia.
authors.forename: Zagorka
authors.initials: Z
authors.lastname: Milovanovic
authors.affiliation: Genetic Laboratory Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Radenkovic
authors.affiliation: High-Risk Pregnancy Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Vuceljic
authors.affiliation: Genetic Laboratory Department, Obstetrics and Gynecology Clinic "Narodni Front", Street Kraljice Natalije 62, 11000 Belgrade, Serbia. Electronic address: ivanajoksic@yahoo.com.
authors.forename: Ivana
authors.initials: I
authors.lastname: Joksic
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D048749:Cytokinesis; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D016640:Diabetes, Gestational / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008750:Methyldopa / Q000494:pharmacology / Q000627:therapeutic use; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D018384:Oxidative Stress; D011247:Pregnancy; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D055815:Young Adult
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D008750:Methyldopa; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine; Cytokinesis-block micronucleus cytome assay; Gestational arterial hypertension; Gestational diabetes mellitus; Malondialdehyde; Methyldopa; Micronuclei; Oxidative stress