title: KChIP2 regulates the cardiac Ca2+ transient and myocyte contractility by targeting ryanodine receptor activity.
abstract: Pathologic electrical remodeling and attenuated cardiac contractility are featured characteristics of heart failure. Coinciding with these remodeling events is a loss of the K+ channel interacting protein, KChIP2. While, KChIP2 enhances the expression and stability of the Kv4 family of potassium channels, leading to a more pronounced transient outward K+ current, Ito,f, the guinea pig myocardium is unique in that Kv4 expression is absent, while KChIP2 expression is preserved, suggesting alternative consequences to KChIP2 loss. Therefore, KChIP2 was acutely silenced in isolated guinea pig myocytes, which led to significant reductions in the Ca2+ transient amplitude and prolongation of the transient duration. This change was reinforced by a decline in sarcomeric shortening. Notably, these results were unexpected when considering previous observations showing enhanced ICa,L and prolonged action potential duration following KChIP2 loss, suggesting a disruption of fundamental Ca2+ handling proteins. Evaluation of SERCA2a, phospholamban, RyR, and sodium calcium exchanger identified no change in protein expression. However, assessment of Ca2+ spark activity showed reduced spark frequency and prolonged Ca2+ decay following KChIP2 loss, suggesting an altered state of RyR activity. These changes were associated with a delocalization of the ryanodine receptor activator, presenilin, away from sarcomeric banding to more diffuse distribution, suggesting that RyR open probability are a target of KChIP2 loss mediated by a dissociation of presenilin. Typically, prolonged action potential duration and enhanced Ca2+ entry would augment cardiac contractility, but here we see KChIP2 fundamentally disrupts Ca2+ release events and compromises myocyte contraction. This novel role targeting presenilin localization and RyR activity reveals a significance for KChIP2 loss that reflects adverse remodeling observed in cardiac disease settings.
authors.affiliation: Heart and Vascular Research Center, Department of Medicine, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America.|Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, United States of America.
authors.forename: Drew M
authors.initials: DM
authors.lastname: Nassal
authors.affiliation: Heart and Vascular Research Center, Department of Medicine, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wan
authors.affiliation: Heart and Vascular Research Center, Department of Medicine, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Heart and Vascular Research Center, Department of Medicine, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Laurita
authors.affiliation: Heart and Vascular Research Center, Department of Medicine, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America.|Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, United States of America.
authors.forename: Isabelle
authors.identifier: http://orcid.org/0000-0002-1812-7267
authors.initials: I
authors.lastname: Deschênes
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D006168:Guinea Pigs; D007150:Immunohistochemistry; D051605:Kv Channel-Interacting Proteins / Q000235:genetics / Q000502:physiology*; D032342:Muscle Cells / Q000378:metabolism*; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*
chemical_list: D051605:Kv Channel-Interacting Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium