title: Cryopreservation Of Nili-Ravi Buffalo Bull Sperm in Cryodiluent Supplemented with Lolium perenne Protein Preparations.
abstract: BACKGROUND
  Semen from the Nili-Ravi buffalo bull, Bubalus bubalis, shows poor survival after freeze storage compared to bovine (Bos taurus and Bos indicus) semen. Freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes.
  
  
  METHODS
  We measured the impact of protein preparations derived from a frost-resistant perennial grass, Lolium perenne, with ice recrystallization inhibition activity on the low temperature storage of B. bubalis semen.
  
  
  RESULTS
  When the L. perenne preparations (0.1, 1, 10 µg/mL) were added to buffalo semen [2 ejaculates per bull (N=3) per replicate (r=3)] in Tris-citrate extender (50×106sperm mL-1), there was no impact on semen quality, as measured by sperm motility and plasma membrane integrity, after storage at 4 degree C (P>0.05). However, when semen supplemented with the grass proteins (0.1 and 1 µg mL-1) was evaluated after freezing and storage in liquid nitrogen for 24 h, post-thaw sperm progressive motility and plasma membrane integrity was higher (P<0.05) than in control samples. Post-thaw sperm viability and sperm acrosome integrity was similar (P > 0.05) to controls.
  
  
  CONCLUSIONS
  The improvement in cryopreserved buffalo sperm progressive motility and plasma membrane integrity suggests that the use of these easily-made preparations may improve fertility after cryopreservation and offers the prospect of improved conception rates after artificial insemination with cryopreservation.
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Qadeer
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: M S
authors.initials: MS
authors.lastname: Ansari
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: B A
authors.initials: BA
authors.lastname: Rakha
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: R
authors.initials: R
authors.lastname: Ejaz
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: A U
authors.initials: AU
authors.lastname: Husna
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Azam
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: N
authors.initials: N
authors.lastname: Ullah
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario, Canada.
authors.forename: V K
authors.initials: VK
authors.lastname: Walker
authors.affiliation: Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. shamim@uaar.edu.pk.
authors.forename: S
authors.initials: S
authors.lastname: Akhter
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D008129:Lolium / Q000737:chemistry; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D010940:Plant Proteins / Q000494:pharmacology; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D010936:Plant Extracts; D010940:Plant Proteins