title: Synthetic glyco-O-sulfatome for profiling of human natural antibodies.
abstract: Our understanding of biological role of glycans O-sulfation remains at the level of beginners due to microheterogeneity, lability and other difficulties of exact structural assignment. Partially, problem of functional investigations, especially determination of glycoepitope specificity of carbohydrate-binding proteins could be solved with the help of synthetic glycans of certain structure. Here we summing up our synthetic efforts in creation of synthetic O-sulfatome, and bring together all the synthesized in our group sulfated glycans, both existing in nature, yet undiscovered but biochemically licit, and completely unnatural. All glycans have aminoalkyl spacer group allowing immobilization on a chip. We exemplify the capabilities of O-sulfoglycan microarray (containing >70 ligands) for profiling human natural antibodies; for a number of glycans O-sulfation dramatically changes interaction with human antibodies.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Galina
authors.initials: G
authors.lastname: Pazynina
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Marina
authors.initials: M
authors.lastname: Sablina
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Ovchinnikova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tyrtysh
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tsygankova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Alexander
authors.initials: A
authors.lastname: Tuzikov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Kira
authors.initials: K
authors.lastname: Dobrochaeva
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Shilova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation.
authors.forename: Nailia
authors.initials: N
authors.lastname: Khasbiullina
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, ul. Miklukho-Maklaya 16/10, Moscow, 11797, Russian Federation. Electronic address: professorbovin@yandex.ru.
authors.forename: Nicolai
authors.initials: N
authors.lastname: Bovin
mesh_terms: D000906:Antibodies / Q000276:immunology*; D002240:Carbohydrate Sequence; D060326:Chemistry Techniques, Synthetic; D006801:Humans; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D000906:Antibodies; D011134:Polysaccharides; D013431:Sulfates
keywords: Anti-glycan antibodies; Glycan synthesis; Glycome; Natural antibodies; O-sulfates; Sulfatome