title: Proteomics of mammalian mitochondria in health and malignancy: From protein identification to function.
abstract: The mitochondrial set of proteins is a dynamic system, crucial for multiple functions of this organelle. Differential expression of genes in various tissues, alternative splicing, post-translational modifications, turnover and spatial dynamics of proteins are the factors that influence mitochondrial proteomes increasing their versatility. A wide range of high-throughput proteomic approaches are extensively used for identification, quantification and functional assessment of human and other mammalian mitochondrial proteins. This article reviews the methods and approaches which can be utilized for achieving one or another specific goal in mitochondrial investigations, and the recent advances in application of proteomics to study the roles of mitochondria in tumorigenesis and cancer progression.
authors.affiliation: Laboratory of Biomedical Research, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 bd. 2 Leninsky Prospekt, 119071 Moscow, Russia. Electronic address: leryomina1@gmail.com.
authors.forename: Lidia
authors.initials: L
authors.lastname: Eremina
authors.affiliation: Laboratory of Biomedical Research, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 bd. 2 Leninsky Prospekt, 119071 Moscow, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Pashintseva
authors.affiliation: Laboratory of Biomedical Research, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 bd. 2 Leninsky Prospekt, 119071 Moscow, Russia.
authors.forename: Leonid
authors.initials: L
authors.lastname: Kovalev
authors.affiliation: Laboratory of Biomedical Research, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 bd. 2 Leninsky Prospekt, 119071 Moscow, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Kovaleva
authors.affiliation: Laboratory of Biomedical Research, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 bd. 2 Leninsky Prospekt, 119071 Moscow, Russia; Department of Biochemistry, Medical Institute, Peoples' Friendship University of Russia, 6 Miklukho-Maklaya Street, 117198 Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Shishkin
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D024101:Mitochondrial Proteins
keywords: Cancer; Mass spectrometry; Mitochondria; Proteomics