title: Deficiencies of Circulating Mucosal-associated Invariant T Cells and Natural Killer T Cells in Patients with Multiple Trauma.
abstract: Mucosal-associated invariant T (MAIT) cells and natural killer T (NKT) cells are known to play important roles in autoimmunity, infectious diseases and cancers. However, little is known about the roles of these invariant T cells in multiple trauma. The purposes of this study were to examine MAIT and NKT cell levels in patients with multiple trauma and to investigate potential relationships between these cell levels and clinical parameters. The study cohort was composed of 14 patients with multiple trauma and 22 non-injured healthy controls (HCs). Circulating MAIT and NKT cell levels in the peripheral blood were measured by flow cytometry. The severity of injury was categorised according to the scoring systems, such as Acute Physiology and Chronic Health Evaluation (APACHE) II score, Simplified Acute Physiology Score (SAPS) II, and Injury Severity Score (ISS). Circulating MAIT and NKT cell numbers were significantly lower in multiple trauma patients than in HCs. Linear regression analysis showed that circulating MAIT cell numbers were significantly correlated with age, APACHE II, SAPS II, ISS category, hemoglobin, and platelet count. NKT cell numbers in the peripheral blood were found to be significantly correlated with APACHE II, SAPS II, and ISS category. This study shows numerical deficiencies of circulating MAIT cells and NKT cells in multiple trauma. In addition, these invariant T cell deficiencies were found to be associated with disease severity. These findings provide important information for predicting the prognosis of multiple trauma.
authors.affiliation: Department of Surgery, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Young Goun
authors.identifier: http://orcid.org/0000-0002-4418-2729
authors.initials: YG
authors.lastname: Jo
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Hyun Jung
authors.identifier: http://orcid.org/0000-0003-2901-5962
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Surgery, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Jung Chul
authors.identifier: http://orcid.org/0000-0002-6774-1861
authors.initials: JC
authors.lastname: Kim
authors.affiliation: Department of Rheumatology, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Young Nan
authors.identifier: http://orcid.org/0000-0001-6922-8570
authors.initials: YN
authors.lastname: Cho
authors.affiliation: Department of Rheumatology, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Jeong Hwa
authors.identifier: http://orcid.org/0000-0002-6535-6230
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Department of Rheumatology, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Hye Mi
authors.identifier: http://orcid.org/0000-0002-3622-5995
authors.initials: HM
authors.lastname: Jin
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Seung Jung
authors.identifier: http://orcid.org/0000-0001-9708-5837
authors.initials: SJ
authors.lastname: Kee
authors.affiliation: Department of Rheumatology, Chonnam National University Medical School and Hospital, Gwangju, Korea. parkyw@jnu.ac.kr.
authors.forename: Yong Wook
authors.identifier: http://orcid.org/0000-0002-6937-7119
authors.initials: YW
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D000072336:Mucosal-Associated Invariant T Cells / Q000166:cytology* / Q000276:immunology; D009104:Multiple Trauma / Q000097:blood / Q000276:immunology / Q000473:pathology*; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000276:immunology; D012720:Severity of Illness Index
chemical_list: D006454:Hemoglobins
keywords: Flow Cytometry; Mucosal-Associated Invariant T Cells; Multiple Trauma; Natural Killer T Cells