title: EGFR-induced phosphorylation of type Iγ phosphatidylinositol phosphate kinase promotes pancreatic cancer progression.
abstract: Pancreatic cancer is one of the deadliest malignancies and effective treatment has always been lacking. In current study, we investigated how the type Iγ phosphatidylinositol phosphate kinase (PIPKIγ) participates in the progression of pancreatic ductal adenocarcinoma (PDAC) for novel therapeutic potentials against this lethal disease. We found that PIPKIγ is up-regulated in all tested PDAC cell lines. The growth factor (including EGFR)-induced tyrosine phosphorylation of PIPKIγ is significantly elevated in in situ and metastatic PDAC tissues. Loss of PIPKIγ inhibits the aggressiveness of PDAC cells by restraining the activities of AKT and STAT3, as well as MT1-MMP expression. Therefore when planted into the pancreas of nude mice, PIPKIγ-depleted PDAC cells exhibits substantially repressed tumor growth and metastasis comparing to control PDAC cells. Results from further studies showed that the phosphorylation-deficient PIPKIγ mutant, unlike its wild-type counterpart, cannot rescue PDAC progression inhibited by PIPKIγ depletion. These findings indicate that PIPKIγ, functioning downstream of EGFR signaling, is critical to the progression of PDAC, and suggest that PIPKIγ is potentially a valuable therapeutic target for PDAC treatment.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester MN, USA.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester MN, USA.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors.forename: Juemin
authors.initials: J
authors.lastname: Fang
authors.affiliation: Shanghai East Hospital, Tongji University, Shanghai, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester MN, USA.
authors.forename: Xunhao
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester MN, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Shanghai East Hospital, Tongji University, Shanghai, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester MN, USA.
authors.forename: Kun
authors.initials: K
authors.lastname: Ling
mesh_terms: D000818:Animals; D021441:Carcinoma, Pancreatic Ductal / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D018450:Disease Progression; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C020077:1-phosphatidylinositol-4-phosphate 5-kinase; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; metastasis; pancreatic cancer; type Iγ phosphatidylinositol phosphate kinase; tyrosine phosphorylation