title: In vitro tracking and intracellular protein distribution in immunology.
abstract: New imaging techniques have enabled major advances in understanding how immune reactions are initiated, coordinated and controlled. Imaging methods, which were previously mostly descriptive and supplementary to more quantitative approaches, have now reached sufficient precision and throughput that they are becoming integral to almost all aspects of immunology research. Imaging methodologies that increase the resolution and sensitivity of detection, alongside an ever-expanding range of fluorescent reporters of molecular and cellular activity, and vastly improved analysis methods, have all facilitated this transformation. In this review, we will discuss how advances in imaging are changing the way we view immune activation and control using T cells as the model immune system. We will describe how imaging has transformed our knowledge of molecular and signalling events in T-cell activation, and the impact of these molecular events on the behaviour of T cells.
authors.affiliation: Centre for Micro-Photonics, Faculty of Science, Engineering &Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia.
authors.forename: Kajal
authors.initials: K
authors.lastname: Zibaei
authors.affiliation: Centre for Micro-Photonics, Faculty of Science, Engineering &Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia.|Immune Signalling Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia.|Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.|Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Russell
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D042541:Intracellular Space / Q000378:metabolism; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods; D021381:Protein Transport; D015398:Signal Transduction; D059008:Time-Lapse Imaging