title: Dual crosslinked chondroitin sulfate injectable hydrogel formed via continuous Diels-Alder (DA) click chemistry for bone repair.
abstract: In the present work, a thermosensetive copolymer with a low gelation concentration under 37°C, F127@ChS (F127 crosslinked chondroitin sulfate) was synthesized via DA click chemistry between F127-AMI (maleimido terminated F127) and ChS-furan (furfurylamine grafted chondroitin sulfate). Then, dual crosslinked hydrogels were prepared based on F127@ChS and PEG-AMI (maleimido terminated polyethylene glycol). The physical crosslinking of F127@ChS affords the hydrogel fast gelation behavior, while in situ DA click reaction occurred between F127@ChS and PEG-AMI affords the hydrogel system covalent crosslinking. The dual crosslinked injectable hydrogel was applied as scaffold to load BMP-4 for rat cranial defect repair. As indicated by X-ray imaging, cranial digital images and histological (HE and Masson) staining analysis, new bone tissues were formed in the defected area after 12 weeks repair. The results demonstrate that the novel dual crosslinked injectable hydrogel offer an interesting option for cranial bone tissue engineering.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China. Electronic address: lshy@lzu.edu.cn.
authors.forename: Shaoyu
authors.initials: S
authors.lastname: Lü
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Gansu Tobacco Industrial Co., Ltd., Lanzhou 730050, People's Republic of China.
authors.forename: Boli
authors.initials: B
authors.lastname: Ni
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Piao
authors.initials: P
authors.lastname: Ning
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China. Electronic address: mzliu@lzu.edu.cn.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002809:Chondroitin Sulfates / Q000737:chemistry*; D057930:Click Chemistry; D020100:Hydrogels / Q000737:chemistry*; D011092:Polyethylene Glycols; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D011092:Polyethylene Glycols; D002809:Chondroitin Sulfates
keywords: Cranial bone tissue engineering; Diels-Alder; Dual crosslinked; Injectable hydrogel; Scaffold