title: Approaches of the Transcriptomic Analysis in Astrocytes: Potential Pharmacological Targets.
abstract: Astrocytes are important glial cells involved in the ionic regulation of the extracellular fluid in the Central Nervous System (CNS), the formation of the blood brain barrier (BBB) and the support to neurons for the maintenance of the Krebs cycle intermediaries. Even though these cells are known to be important for the brain functioning, several of their functions and their development have not been fully elucidated. In this context, identifying the algorithms used for their analysis plays a pivotal role in the development of new strategies in the study of astrocytes. The main objective of this review is to summarize the techniques that have helped to obtain transcriptomic data in astrocytes and the new algorithms that were used to perform the analysis of experimental data, elucidating new studies in which these had been used. We also highlight the current transcriptomics approaches targeting astrocytes function as a possible target for pharmacological interventions.
authors.affiliation: Departamento de Nutricion y Bioquimica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogota D.C. Colombia.
authors.forename: George E
authors.initials: GE
authors.lastname: Barreto
authors.affiliation: Fundacion NeuroRegeneracion en Colombia, Bogota. Colombia.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Gomez
authors.affiliation: Universidad La Sabana, Bogota. Colombia.
authors.forename: Rosa Helena
authors.initials: RH
authors.lastname: Bustos
authors.affiliation: Universidad Antonio Narino, Bogota. Colombia.
authors.forename: Diego A
authors.initials: DA
authors.lastname: Forero
authors.affiliation: University of Atlanta, John Greek, GA. United States.
authors.forename: Gjumrakch
authors.initials: G
authors.lastname: Aliev
authors.affiliation: Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, 19991 Moscow. Russian Federation.
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Tarasov
authors.affiliation: Department of Biochemistry and Bioinformatics, Institute of Science, GITAM University, Visakhapatnam-530 045, Andhra Pradesh. India.
authors.forename: Nagendra S
authors.initials: NS
authors.lastname: Yarla
authors.affiliation: Fac. Cs de la Salud, Universidad San Sebastiasn, Lientur 1457, Concepcion, 4080871. Chile.
authors.forename: Valentina
authors.initials: V
authors.lastname: Echeverria
authors.affiliation: Departamento de Nutricion y Bioquimica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogota D.C. Colombia.
authors.forename: Janneth
authors.initials: J
authors.lastname: Gonzalez
mesh_terms: D000465:Algorithms; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism; D015195:Drug Design; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009474:Neurons / Q000378:metabolism; D059467:Transcriptome
keywords: Astrocytes; blood brain barrier; brain; central nervous system; neurological disorders; transcriptome methods