title: Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy.
abstract: Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress. J. Cell. Biochem. 118: 3920-3931, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: CIBICI-CONICET, Faculty of Chemical Sciences, Department of Clinical Biochemistry, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Suarez
authors.affiliation: Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida.
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Piqueras
authors.affiliation: Department of Ophthalmology, University Clinic Reina Fabiola, Universidad Catolica de Cordoba, Cordoba, Argentina.
authors.forename: Leandro
authors.initials: L
authors.lastname: Correa
authors.affiliation: Department of Ophthalmology, University Clinic Reina Fabiola, Universidad Catolica de Cordoba, Cordoba, Argentina.
authors.forename: Evangelina
authors.initials: E
authors.lastname: Esposito
authors.affiliation: Department of Ophthalmology, University Clinic Reina Fabiola, Universidad Catolica de Cordoba, Cordoba, Argentina.
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Barros
authors.affiliation: Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida.
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhattacharya
authors.affiliation: Department of Ophthalmology, University Clinic Reina Fabiola, Universidad Catolica de Cordoba, Cordoba, Argentina.
authors.forename: Julio A
authors.initials: JA
authors.lastname: Urrets-Zavalia
authors.affiliation: CIBICI-CONICET, Faculty of Chemical Sciences, Department of Clinical Biochemistry, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.forename: Horacio M
authors.identifier: 0000-0003-4979-8642
authors.initials: HM
authors.lastname: Serra
mesh_terms: D003315:Cornea / Q000378:metabolism* / Q000473:pathology; D003316:Corneal Diseases / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010743:Phospholipids / Q000378:metabolism*
chemical_list: D010743:Phospholipids
keywords: CDK; CORNEA; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLSERINE; PHOSPHOLIPIDS; TANDEM MASS SPECTROMETRY