title: Cytotoxicity and Apoptosis Induction ofArdisia crispaand Its Solvent Partitions againstMus musculusMammary Carcinoma Cell Line (4T1).
abstract: This study was conducted to investigate the cytotoxicity and apoptosis effect ofA. crispaextract and its solvent partition (ethyl acetate and aqueous extract) againstMus musculusmammary carcinoma cell line (4T1). The normal mouse fibroblast cell line (NIH3T3) was used as comparison for selective cytotoxicity properties. The cytotoxicity evaluation was assessed using MTT assay. AO/PI dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis. Results showed that 80% methanol extract from leaves showed most promising antimammary cancer agent with IC50 value of 42.26 ± 1.82μg/mL and selective index (SI) value of 10.22. Ethyl acetate was cytotoxic for both cancer and normal cell while aqueous extract exhibited poor cytotoxic effect. 4T1 cells labelled with AO/PI and Annexin V-FITC and treated with 80% methanol extract demonstrated that the extract induces apoptosis to 4T1 mammary cancer cells. In conclusion, 80% methanol extract ofA. crispawas selectively cytotoxic towards 4T1 cells but less cytotoxic towards NIH3T3 cells and induced the cancerous cells into apoptotic stage as early as 6 hours.
authors.affiliation: Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Muhammad Luqman
authors.initials: ML
authors.lastname: Nordin
authors.affiliation: Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Arifah
authors.identifier: 0000-0002-6132-2877
authors.initials: A
authors.lastname: Abdul Kadir
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Zainul Amiruddin
authors.initials: ZA
authors.lastname: Zakaria
authors.affiliation: Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia; Prof. Fauziah Biohealth Sdn. Bhd, Malaysia.
authors.forename: Fauziah
authors.initials: F
authors.lastname: Othman
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Rasedee
authors.identifier: 0000-0001-6625-508X
authors.initials: R
authors.lastname: Abdullah
authors.affiliation: Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Muhammad Nazrul Hakim
authors.initials: MN
authors.lastname: Abdullah