title: Haemoglobin site-specifically modified with ferulic acid to suppress the autoxidation.
abstract: Haemoglobin site-specifically modified with ferulic acid (FA-Hb), was synthesized. The effects on the characteristic absorption and the oxygen saturation curve of FA-Hb were investigated by UV-Vis spectrophotometry and oxygen analyzer. Furthermore, the autoxidation rates of Hb and FA-Hb were also discussed with or without sodium azide in the system. The results showed that there was no obvious transformation of the heme structure of FA-Hb. It was also demonstrated that the oxygen-carrying capacity of FA-Hb was similar to the natural Hb, but the autoxidation rate of FA-Hb was much lower than Hb in different systems.
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Qi
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.affiliation: a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
mesh_terms: D003373:Coumaric Acids / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction
chemical_list: D003373:Coumaric Acids; D006454:Hemoglobins; C004999:ferulic acid
keywords: Haemoglobin; MetHb; ROS; antioxidation; ferulic acid