title: Phosphoprotein/chitosan electrospun nanofibrous scaffold for biomineralization.
abstract: In this study, negatively charged phosvitin (PV) and positively charged chitosan (CS) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. Morphologies of the LBL films coating mats were observed by scanning electron microscope (SEM). Afterwards, in vitro biomimetic mineralization was carried out through incubation of the fibrous mats in a simulated body fluid (SBF) solution for different time. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) were used to characterize the morphology and structure of the deposited mineral phase on the scaffolds. In addition, the cell culture experiment demonstrated that the scaffolds with the LBL structured films were of good cell compatibility for MC3T3-E1 cells. Moreover, the cell proliferation was affected by the number of deposition layers and the composition of outer-most layer. Confocal laser scanning microscopy (CLSM) and SEM imaging revealed a good performance of cell adhesion and spreading of MC3T3-E1 cells on the surface of biocomposite scaffold. So CS/PV nanofibrous mats were satisfactory for the composite to be used in bioapplications.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Liang
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, The College of Life Sciences, Hubei University, Wuhan 430062, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sheng
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, LinGang New City, Shanghai 201306, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Pei
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China. Electronic address: lijingfood@mail.hzau.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004560:Electricity; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D010750:Phosphoproteins; D048271:Chitosan
keywords: Biocompatibility; Biomineralization; Chitosan; Electrospun; Phosvitin