title: The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes.
abstract: Chemical and mechanical cues from the cerebrospinal fluid (CSF) can affect the development and function of the central nervous system (CNS). How such cues are detected and relayed to the CNS remains elusive. Cerebrospinal fluid-contacting neurons (CSF-cNs) situated at the interface between the CSF and the CNS are ideally located to convey such information to local networks. In the spinal cord, these GABAergic neurons expressing the PKD2L1 channel extend an apical extension into the CSF and an ascending axon in the spinal cord. In zebrafish and mouse spinal CSF-cNs originate from two distinct progenitor domains characterized by distinct cascades of transcription factors. Here we ask whether these neurons with different developmental origins differentiate into cells types with different functional properties. We show in zebrafish larva that the expression of specific markers, the morphology of the apical extension and axonal projections, as well as the neuronal targets contacted by CSF-cN axons, distinguish the two CSF-cN subtypes. Altogether our study demonstrates that the developmental origins of spinal CSF-cNs give rise to two distinct functional populations of sensory neurons. This work opens novel avenues to understand how these subtypes may carry distinct functions related to development of the spinal cord, locomotion and posture.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.|Muséum National d'Histoire Naturelle, Paris, 75005, France.
authors.forename: Lydia
authors.initials: L
authors.lastname: Djenoune
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Desban
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Johanna
authors.identifier: http://orcid.org/0000-0002-9557-1353
authors.initials: J
authors.lastname: Gomez
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Jenna R
authors.initials: JR
authors.lastname: Sternberg
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Andrew
authors.initials: A
authors.lastname: Prendergast
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Langui
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.|Muséum National d'Histoire Naturelle, Paris, 75005, France.
authors.forename: Feng B
authors.initials: FB
authors.lastname: Quan
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Hugo
authors.initials: H
authors.lastname: Marnas
authors.affiliation: Institut Curie, Paris, 75005, France.|Thomas O Auer, Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Auer
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Rio
authors.affiliation: Institut Curie, Paris, 75005, France.
authors.forename: Filippo
authors.identifier: http://orcid.org/0000-0001-8551-2846
authors.initials: F
authors.lastname: Del Bene
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France. pierreluc.bardet@icm-institute.org.
authors.forename: Pierre-Luc
authors.initials: PL
authors.lastname: Bardet
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, Inserm, CNRS, AP-HP, Institut du Cerveau et de la Moelle épinière (ICM) - Hôpital Pitié-Salpêtrière, Boulevard de l'hôpital, F-75013, Paris, France. claire.wyart@icm-institute.org.
authors.forename: Claire
authors.identifier: http://orcid.org/0000-0002-1668-4975
authors.initials: C
authors.lastname: Wyart
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D015415:Biomarkers; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal; D006720:Homozygote; D009154:Mutation; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D011984:Sensory Receptor Cells / Q000502:physiology / Q000648:ultrastructure; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology*; D013126:Spinal Nerve Roots; D050396:TRPP Cation Channels; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; C547527:PKD2 protein, zebrafish; D050396:TRPP Cation Channels; D029961:Zebrafish Proteins