title: Molecular and genomic features ofMycobacterium bovisstrain 1595 isolated from Korean cattle.
abstract: The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis ofMycobacterium (M.) bovisstrain 1595 isolated from a native Korean cow. Molecular typing showed thatM. bovis1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type ofM. bovisin Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes ofMycobacterium tuberculosiscomplex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed thatM. bovisAF2122/97 was the strain with the greatest number of completely matched proteins toM. bovis1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits amongM. bovisisolates in Korea.
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.|Department of Integrated Biomedical and Life Science, College of Health Science, Korea University, Seoul 02841, Korea.
authors.forename: Narae
authors.initials: N
authors.lastname: Kim
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Yunho
authors.initials: Y
authors.lastname: Jang
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Jin Kyoung
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Soyoon
authors.initials: S
authors.lastname: Ryoo
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Ka Hee
authors.initials: KH
authors.lastname: Kwon
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Miso
authors.initials: M
authors.lastname: Kim
authors.affiliation: Chungcheongbukdo Veterinary Service, Chungju 27492, Korea.
authors.forename: Shin Seok
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Chungcheongbukdo Veterinary Service, Chungju 27492, Korea.
authors.forename: Hyeon Seop
authors.initials: HS
authors.lastname: Byeon
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Hee Soo
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Public Health Science (Brain Korea 21 PLUS Program), Graduate School, Korea University, Seoul 02841, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
authors.affiliation: Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyang 14089, Korea.
authors.forename: Jae-Myung
authors.initials: JM
authors.lastname: Kim
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology; D016680:Genome, Bacterial / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics; D009163:Mycobacterium bovis / Q000235:genetics*; D056910:Republic of Korea; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000382:microbiology*
keywords: Korea; Mycobacterium bovis; cattle; genomics