title: Identification of urine biomarkers associated with lung adenocarcinoma.
abstract: Lung adenocarcinoma (LAC) progression is accompanied by changes in protein levels that may be reflected in body fluids, such as urine. Urine collected from LAC patients (n=34) and healthy controls (n=36) was analyzed via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange magnetic beads. The results revealed 76 urinary polypeptides significantly different between LAC patients and normal controls (P<0.05). Twenty-two of these peptides were up-regulated and 54 were down-regulated. Thirteen peptides had average peak intensities >600. Twelve of these 13 peptides were successfully identified using nano-liquid chromatography-tandem MS. Receiver operating characteristic analyses identified seven peptides with superior LAC diagnostic performances. Immunohistochemical staining in 20 paired LAC and adjacent normal tissues showed that IGKC, AAT, SH3BGRL3, osteopontin and gelsolin levels were higher in LAC tissues than in adjacent tissuesand were closely associated with LAC. Urinary peptides assessments may thus provide a novel, noninvasive, repeatable method for detecting and monitoring LAC. New, low-cost detection methods and bioinformatics tools are therefore urgently needed for the analysis of low abundance proteins and peptides in body fluids.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pulmonary and Critical Care Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.|Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000652:urine*; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000652:urine*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000652:urine*; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor
keywords: MALDI-TOF MS; biomarker; immunohistochemistry; lung adenocarcinoma; urine peptides