title: Molecular mechanism of bacterial Hsp90 pH-dependent ATPase activity.
abstract: Hsp90 is a dimeric molecular chaperone that undergoes an essential and highly regulated open-to-closed-to-open conformational cycle upon ATP binding and hydrolysis. Although it has been established that a large energy barrier to closure is responsible for Hsp90's low ATP hydrolysis rate, the specific molecular contacts that create this energy barrier are not known. Here we discover that bacterial Hsp90 (HtpG) has a pH-dependent ATPase activity that is unique among other Hsp90 homologs. The underlying mechanism is a conformation-specific electrostatic interaction between a single histidine, H255, and bound ATP. H255 stabilizes ATP only while HtpG adopts a catalytically inactive open configuration, resulting in a striking anti-correlation between nucleotide binding affinity and chaperone activity over a wide range of pH. Linkage analysis reveals that the H255-ATP salt bridge contributes 1.5 kcal/mol to the energy barrier of closure. This energetic contribution is structurally asymmetric, whereby only one H255-ATP salt-bridge per dimer of HtpG controls ATPase activation. We find that a similar electrostatic mechanism regulates the ATPase of the endoplasmic reticulum Hsp90, and that pH-dependent activity can be engineered into eukaryotic cytosolic Hsp90. These results reveal site-specific energetic information about an evolutionarily conserved conformational landscape that controls Hsp90 ATPase activity.
authors.affiliation: Department of Biochemistry, Brandeis University, Waltham, Massachusetts, 02453.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Biochemistry, Brandeis University, Waltham, Massachusetts, 02453.
authors.forename: Reyal S
authors.initials: RS
authors.lastname: Hoxie
authors.affiliation: Department of Biochemistry, Brandeis University, Waltham, Massachusetts, 02453.
authors.forename: Timothy O
authors.initials: TO
authors.lastname: Street
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics; D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000072417:Protein Domains; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary
chemical_list: D029968:Escherichia coli Proteins; D018841:HSP90 Heat-Shock Proteins; C489352:HtpG protein, E coli; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases
keywords: ATP; Hsp90; chaperone; pH