title: Optimization of Ionic Liquid-Assisted Extraction of Biflavonoids from Selaginella doederleinii and Evaluation of Its Antioxidant and Antitumor Activity.
abstract: As new green solvents, ionic liquids (ILs) have been generally applied in the extraction and separation of natural product. In this study, microwave assisted extraction based on IL (IL-MAE) was firstly employed to extract total biflavonoids fromSelaginella doederleinii. Based on single-factor experiment, microwave power (300-700 W), extract time (30-50 min) and extract temperature (40-60 °C) on total bioflavonoids and antioxidant activities of the extracts were further investigated by a Box-Behnken design of response surface methodology (RSM) selecting total bioflavonoids yields and IC50of radical scavenging as index. Besides antioxidant activity of the extract was evaluated by a 2,2-diphenyl-1-picrylhydarzyl (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) radical scavenging assay, ferric reducing power assay and chelation of ferrous ions assay, and then anticaner activity was also researched against A549 cell line and 7721 cell line. The results illustrated that three factors and their interactions could be well suited for second-order polynomial models (p< 0.05). Through process parameters, optimization of the extract (460 W, 40 min, and 45 °C) and detection of bioactivity, the yield of total bioflavonoids was 16.83 mg/g and IC50value was 56.24 μg/mL, respectively, indicating the extract has better anti-oxidation effect and antitumor activity. Furthermore, IL-MAE was the most efficient extracting method compared with MAE and Soxhlet extraction, which could improve extraction efficiency in a shorter time and at a lower temperature. In general, ILs-MAE was first adopted to establish a novel and green extraction process on the yields of total biflavonoids fromS. doederleinii. In addition, the extract of containing biflavones showed potent antioxidant and anticancer capacity as a utilized valuable bioactive source for natural medicine.
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563003, China. 13984515977@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563003, China. 18785413312@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qian
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563003, China. 18785413632@163.com.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Tian
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563003, China. 18311542078@163.com.
authors.forename: Shi-Meng
authors.initials: SM
authors.lastname: Yuan
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563003, China. 18076271737@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563003, China. wg8855350@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044946:Biflavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005591:Chemical Fractionation / Q000379:methods; D006801:Humans; D020128:Inhibitory Concentration 50; D052578:Ionic Liquids / Q000737:chemistry*; D008872:Microwaves; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D032503:Selaginellaceae / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D044946:Biflavonoids; D052578:Ionic Liquids; D010936:Plant Extracts
keywords: Selaginella doederleinii Hieron; anticancer; antioxidant; flavonoid; ionic liquids