title: Identification of Type III Interferon (IFN-λ) in Chinese Goose: Gene Structure, Age-Dependent Expression Profile, and Antiviral Immune Characteristics In Vivo and In Vitro.
abstract: Type III interferons (IFN-λ1/λ2/λ3, also known as IL-29/28A/28B, and IFN-λ4) are a recently discovered interferon group. In this study, we first identified the Chinese goose IFN-λ (goIFN-λ). The full-length sequence of goIFN-λ was found to be 823 bp. There was only one open reading frame that contained 570 bp, and, encoded 189 amino acids. The predicted goIFN-λ protein showed 78%, 67%, and 40% amino acid identity with duIFN-λ, chIFN-λ, and hIFN-λ3, respectively. The tissue distribution of goIFN-λ existed as a parallel distribution with goIFNLR1 as its functional receptor, which was mainly expressed in epithelium-rich tissues, such as lung, gizzard, proventriculus, skin and pancreas, and immune tissues, such as harderian gland and thymus. Furthermore, the immunological characteristics studies of goIFN-λ showed that there was a significant increase in the mRNA at the transcriptional level of goIFN-λ after the peripheral blood mononuclear cells were stimulated with ploy (I:C) and ODN2006, and infected with Gosling plague virus (GPV). In vivo, the mRNA transcriptional level of goIFN-λ increased nearly 20 times in the lung tissue and nearly 40 times in the pancreatic tissue after being artificially infected with H9N2 AIV. It is suggested that goIFN-λ might play a pivotal role in the mucosal immune protection and antiviral defense.
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Shun
authors.initials: S
authors.lastname: Chen
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
authors.forename: Anqi
authors.initials: A
authors.lastname: Wang
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
authors.forename: Lipei
authors.initials: L
authors.lastname: Sun
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Mingshu
authors.initials: M
authors.lastname: Wang
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.affiliation: 2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Dekang
authors.initials: D
authors.lastname: Zhu
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .
authors.forename: Mafeng
authors.initials: M
authors.lastname: Liu
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Kunfeng
authors.initials: K
authors.lastname: Sun
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yang
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.affiliation: 1 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|2 Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, China .|3 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University , Chengdu, China .
authors.forename: Anchun
authors.initials: A
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004372:Ducks; D005777:Geese / Q000235:genetics / Q000276:immunology* / Q000821:virology; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology; D007372:Interferons / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D016366:Open Reading Frames; D009975:Orthomyxoviridae / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
chemical_list: C000615642:ODN2006; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D007372:Interferons
keywords: Chinese goose; IFN-λ; immunological characteristics; tissue distribution profile