title: Stimulation of long-term ammonium nitrogen deposition on methanogenesis by Methanocellaceae in a coastal wetland.
abstract: Atmospheric nitrogen deposition caused by human activities has been receiving much attention. Here, after long-term simulated ammonium and nitrate nitrogen deposition (NH4Cl, KNO3, and NH4NO3) in the Yellow River Delta (YRD), a sensitive coastal wetland ecosystem typified by a distinct wet and dry season, methane fluxes were measured, by adopting a closed static chamber technique. The results showed that deposition of ammonium nitrogen accelerated methane emissions all year round. Ammonium nitrogen deposition transformed the YRD from a methane sink into a source during the dry season. Methanocellaceae is the only methanogen with increased abundance after the application of NH4Cl and NH4NO3, which promoted methane emissions, during the wet season. The findings suggested that Methanocellaceae may facilitate methane emissions in response to increased ammonium nitrogen deposition. Other methanogens might have profited from ammonium supplementation, such as Methanosarcinaceae. Deposition of nitrate nitrogen did not affect methane flux significantly. To the best of our knowledge, this study is the first to show that Methanocellaceae may be responsible for methane production in coastal wetland system. This study highlights the significant effect of ammonium nitrogen and slight effect of nitrate nitrogen on methane emission in the YRD and it will be helpful to understand the microbial mechanism responding to increased nitrogen deposition in the sensitive coastal wetland ecosystem.
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Xie
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jinchao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Guangxuan
authors.initials: G
authors.lastname: Han
authors.affiliation: Binzhou Medical University, Yantai, China. Electronic address: omwang@aliyun.com.
authors.forename: Oumei
authors.initials: O
authors.lastname: Wang
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. Electronic address: fhliu@yic.ac.cn.
authors.forename: Fanghua
authors.initials: F
authors.lastname: Liu
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D002681:China; D008697:Methane / Q000096:biosynthesis*; D019638:Methanomicrobiaceae / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D045483:Rivers; D053833:Wetlands
chemical_list: D064751:Ammonium Compounds; D009584:Nitrogen; D008697:Methane
keywords: Ammonium and nitrate nitrogen; Atmospheric nitrogen deposition; Coastal wetland; Dry-wet alternate; Methane flux; Methanogens