title: Chiral Recognition by Dissolution DNP NMR Spectroscopy of13C-Labeled dl-Methionine.
abstract: A method based on d-DNP NMR spectroscopy to study chiral recognition is described for the first time. The enantiodifferentiation of a racemic metabolite in a millimolar aqueous solution using a chiral solvating agent was performed. Hyperpolarized13C-labeled dl-methionine enantiomers were differently observed with a single-scan13C NMR experiment, while the chiral auxiliary at thermal equilibrium remained unobserved. The method developed entails a step forward in the chiral recognition of small molecules by NMR spectroscopy, opening new possibilities in situations where the sensitivity is limited, for example, when a low concentration of analyte is available or when the measurement of an insensitive nucleus, like13C, is required. The advantages and current limitations of the method, as well as future perspectives, are discussed.
authors.affiliation: Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona , E-08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Eva
authors.identifier: 0000-0002-5507-0108
authors.initials: E
authors.lastname: Monteagudo
authors.affiliation: Departament de Química, Universitat Autònoma de Barcelona , E-08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Virgili
authors.affiliation: Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona , E-08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Teodor
authors.identifier: 0000-0002-1914-2709
authors.initials: T
authors.lastname: Parella
authors.affiliation: Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona , E-08193 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Míriam
authors.identifier: 0000-0002-6919-7417
authors.initials: M
authors.lastname: Pérez-Trujillo