title: ELF18-INDUCED LONG-NONCODING RNA Associates with Mediator to Enhance Expression of Innate Immune Response Genes in Arabidopsis.
abstract: The plant immune response is a complex process involving transcriptional and posttranscriptional regulation of gene expression. Responses to plant immunity are initiated upon the perception of pathogen-associated molecular patterns, including peptide fragment of bacterial flagellin (flg22) or translation elongation factor Tu (elf18). Here, we identify anArabidopsis thalianalong-noncoding RNA, designated ELF18-INDUCED LONG-NONCODING RNA1 (ELENA1), as a factor enhancing resistance againstPseudomonas syringepvtomatoDC3000. ELENA1 knockdown plants show decreased expression ofPATHOGENESIS-RELATED GENE1(PR1) and the plants are susceptible to pathogens. By contrast, plants overexpressing ELENA1 show elevatedPR1expression after elf18 treatment and display a pathogen resistance phenotype. RNA-sequencing analysis of ELENA1-overexpressing plants after elf18 treatment confirms increased expression of defense-related genes compared with the wild type. ELENA1 directly interacts with Mediator subunit 19a (MED19a) and affects enrichment of MED19a on thePR1promoter. These results show that MED19a regulatesPR1expression through ELENA1. Our findings uncover an additional layer of complexity, implicating long-noncoding RNAs in the transcriptional regulation of plant innate immunity.
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065.|Temasek Life Sciences Laboratory, National University of Singapore, 117604, Singapore.
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Seo
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065.
authors.forename: Hai-Xi
authors.initials: HX
authors.lastname: Sun
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065.|Temasek Life Sciences Laboratory, National University of Singapore, 117604, Singapore.
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Park
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065.|Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Chung-Hao
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Shyi-Dong
authors.initials: SD
authors.lastname: Yeh
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065 jasmin@snu.ac.kr chua@mail.rockefeller.edu.
authors.forename: Choonkyun
authors.initials: C
authors.lastname: Jung
authors.affiliation: Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065 jasmin@snu.ac.kr chua@mail.rockefeller.edu.|Temasek Life Sciences Laboratory, National University of Singapore, 117604, Singapore.
authors.forename: Nam-Hai
authors.identifier: http://orcid.org/0000-0002-8991-0355
authors.initials: NH
authors.lastname: Chua
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D057865:Plant Immunity / Q000235:genetics / Q000502:physiology; D044224:Pseudomonas syringae / Q000472:pathogenicity; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D062085:RNA, Long Noncoding; C077262:PR-1 protein, Arabidopsis