title: Bioactive Metabolites from the Deep Subseafloor Fungus Oidiodendron griseum UBOCC-A-114129.
abstract: Four bioactive compounds have been isolated from the fungusOidiodendron griseumUBOCC-A-114129 cultivated from deep subsurface sediment. They were structurally characterized using a combination of LC-MS/MS and NMR analyses as fuscin and its derivatives (dihydrofuscin, dihydrosecofuscin, and secofuscin) and identified as polyketides. Albeit those compounds were already obtained from terrestrial fungi, this is the first report of their production by anOidiodendronspecies and by the deepest subseafloor isolate ever studied for biological activities. We report a weak antibacterial activity of dihydrosecofuscin and secofuscin mainly directed against Gram-positive bacteria (Minimum Inhibitory Concentration (MIC) equal to Minimum Bactericidal Concentration (MBC), in the range of 100 μg/mL). The activity on various protein kinases was also analyzed and revealed a significant inhibition of CDC2-like kinase-1 (CLK1) by dihysecofuscin.
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IBSAM, 6 Rue de l'université, 29000 Quimper, France. marion.navarri@univ-brest.fr.
authors.forename: Marion
authors.initials: M
authors.lastname: Navarri
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IBSAM, 6 Rue de l'université, 29000 Quimper, France. Camille.jegou@univ-brest.fr.
authors.forename: Camille
authors.initials: C
authors.lastname: Jégou
authors.affiliation: COrInt, UMR CNRS 6226 & Université de Rennes, 35043 Rennes, France. arnaud.bondon@univ-rennes1.fr.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bondon
authors.affiliation: COrInt, UMR CNRS 6226 & Université de Rennes, 35043 Rennes, France. sandrine.pottier@univ-rennes1.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Pottier
authors.affiliation: Kinase Inhibitor Specialized Screening facility (KISSf), Station Biologique de Roscoff, Centre National de la Recherche Scientifique (CNRS) USR 3151 (Protein Phosphorylation and Human Diseases), CS 90074, Roscoff CEDEX 29688, France. bach@sb-roscoff.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bach
authors.affiliation: Kinase Inhibitor Specialized Screening facility (KISSf), Station Biologique de Roscoff, Centre National de la Recherche Scientifique (CNRS) USR 3151 (Protein Phosphorylation and Human Diseases), CS 90074, Roscoff CEDEX 29688, France. baratte@sb-roscoff.fr.
authors.forename: Blandine
authors.initials: B
authors.lastname: Baratte
authors.affiliation: Kinase Inhibitor Specialized Screening facility (KISSf), Station Biologique de Roscoff, Centre National de la Recherche Scientifique (CNRS) USR 3151 (Protein Phosphorylation and Human Diseases), CS 90074, Roscoff CEDEX 29688, France. sruchaud@sb-roscoff.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ruchaud
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IBSAM, 6 Rue de l'université, 29000 Quimper, France. Georges.barbier@univ-brest.fr.
authors.forename: Georges
authors.initials: G
authors.lastname: Barbier
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IBSAM, 6 Rue de l'université, 29000 Quimper, France. Gaetan.burgaud@univ-brest.fr.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Burgaud
authors.affiliation: Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IBSAM, 6 Rue de l'université, 29000 Quimper, France. Yannick.fleury@univ-brest.fr.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Fleury
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000378:metabolism*; D001578:Benzopyrans / Q000494:pharmacology; D001685:Biological Factors / Q000494:pharmacology; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods; D061065:Polyketides / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D001578:Benzopyrans; D001685:Biological Factors; D061065:Polyketides; C100026:fuscin
keywords: anti-kinase; antibacterial; bioactivities; deep subseafloor fungi