title: Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp.
abstract: This study aimed to identify predictors of success rate of mesenchymal stem cell (MSC) isolation from human deciduous teeth pulp. A total of 161 deciduous teeth were extracted at the dental clinic of Chang Gung Memorial Hospital. The MSCs were isolated from dental pulps using a standard protocol. In total, 128 colonies of MSCs were obtained and the success rate was 79.5%. Compared to teeth not yielding MSCs successfully, those successfully yielding MSCs were found to have less severe dental caries (no/mild-to-moderate/severe: 63.3/24.2/12.5% versus 12.5/42.4/42.4%,P< 0.001) and less frequent pulpitis (no/yes: 95.3/4.7% versus 51.5/48.5%,P< 0.001). In a multivariate regression model, it was confirmed that the absence of dental caries (OR = 4.741, 95% CI = 1.564-14.371,P= 0.006) and pulpitis (OR = 9.111, 95% CI = 2.921-28.420,P< 0.001) was significant determinants of the successful procurement of MSCs. MSCs derived from pulps with pulpitis expressed longer colony doubling time than pulps without pulpitis. Furthermore, there were higher expressions of proinflammatory cytokines, interleukin- (IL-) 6 and monocyte chemoattractant protein- (MCP-) 1,P< 0.01, and innate immune response [toll-like receptor 1 (TLR1) and TLR8,P< 0.05; TLR2, TLR3, and TLR6,P< 0.01] in the inflamed than noninflamed pulps. Therefore, a carious deciduous tooth or tooth with pulpitis was relatively unsuitable for MSC processing and isolation.
authors.affiliation: Department of Pediatric Dentistry, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Aileen I
authors.initials: AI
authors.lastname: Tsai
authors.affiliation: Department of Periodontics, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Hsiang-Hsi
authors.initials: HH
authors.lastname: Hong
authors.affiliation: Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Wey-Ran
authors.identifier: 0000-0001-6078-1396
authors.initials: WR
authors.lastname: Lin
authors.affiliation: Department of Medical Research, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Jen-Fen
authors.initials: JF
authors.lastname: Fu
authors.affiliation: Department of Clinical Pathology, Far Eastern Memorial Hospital, New Taipei, Taiwan.
authors.forename: Chih-Chun
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Department of Nephrology, China Medical University Hospital and College of Medicine, China Medical University, Taichung, Taiwan.
authors.forename: I-Kuan
authors.initials: IK
authors.lastname: Wang
authors.affiliation: Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Huang
authors.affiliation: Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Cheng-Hao
authors.initials: CH
authors.lastname: Weng
authors.affiliation: Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan.
authors.forename: Ching-Wei
authors.initials: CW
authors.lastname: Hsu
authors.affiliation: Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan; Kidney Research Center, Chang Gung Memorial Hospital, Linkou, Taiwan; Center for Tissue Engineering, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors.forename: Tzung-Hai
authors.identifier: 0000-0002-0907-1505
authors.initials: TH
authors.lastname: Yen
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014094:Tooth, Deciduous / Q000166:cytology*