title: Expression of profilin-1 in endothelial cells of rats with acute myocardial infarction.
abstract: To analyze the expression of profilin-1 in endothelial cells of rats with acute myocardial infarction.
  
  
  
  Twenty adult male Wistar rats were randomly divided into the myocardial infarction (model) group (n=10) and sham-operation (control) group (n=10). The expression of profilin-1 and phosphorylated extracellular signal kinase (pERK1/2) in aortic endothelial cells, indexes of endothelial injury [levels of endothelial microparticles (EMPs) and nitric oxide (NO)], indexes of myocardial injury [cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB)], and mRNA levels of myocardial apoptotic factors (P53, Fas, Bax, and Bcl-2) in rats between the two groups were compared.
  
  
  
  The expression of profilin-1 and pERK1/2 in aortic endothelial cells of rats in the model group was higher than in the control group (p<0.05), the levels of EMPs were increased, and NO levels were lower (p<0.05); cTnT and CK-MB in myocardial tissue, and mRNA of pro-apoptotic factors (P53, Fas, and Bax) were increased, whereas Bcl-2 mRNA was decreased (p<0.05). The protein expression of profilin-1 and pERK1 was positively correlated with the levels of cTnT, CK-MB, EMP, P53, Fas, and Bax, and negatively correlated with the levels of NO and Bcl-2 (p<0.05).
  
  
  
  The high expression of profilin-1 is an important mechanism of acute myocardial infarction, and is expected to become a new target for the treatment of myocardial infarction.
authors.affiliation: Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China. nf9x33@163.com.
authors.forename: P
authors.initials: P
authors.lastname: Hao
authors.forename: K
authors.initials: K
authors.lastname: Fu
authors.forename: S-P
authors.initials: SP
authors.lastname: Wang
authors.forename: C-Y
authors.initials: CY
authors.lastname: Ma
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Xu
authors.forename: F-Y
authors.initials: FY
authors.lastname: Cao
authors.forename: J-H
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D010766:Phosphorylation; D051304:Profilins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020107:Troponin T / Q000378:metabolism
chemical_list: C495740:Pfn1 protein, rat; D051304:Profilins; D020107:Troponin T; D009569:Nitric Oxide; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D003402:Creatine Kinase