title: Gene and protein expression of a soluble form of CTLA-4 in a healthy dog.
abstract: Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
authors.affiliation: Veterinary Medical Center, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Michihito
authors.initials: M
authors.lastname: Tagawa
authors.affiliation: Veterinary Medical Center, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Veterinary Medical Center, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Genya
authors.initials: G
authors.lastname: Shimbo
authors.affiliation: Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Iguchi
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Tomihari
authors.affiliation: Veterinary Medical Center, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Kazuro
authors.initials: K
authors.lastname: Miyahara
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D060908:CTLA-4 Antigen / Q000097:blood / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D015870:Gene Expression
chemical_list: D060908:CTLA-4 Antigen
keywords: CD152; CTLA-4; dog; soluble form; splicing variant