title: A cell-printing approach for obtaining hASC-laden scaffolds by using a collagen/polyphenol bioink.
abstract: In the cell-printing process, bioink has been considered as an extremely important component for successful fabrication of macroscale cell-laden structures. Bioink should be non-toxic, biocompatible, and printable. To date, alginate has been widely used as a whole or partial component of bioink because it is non-toxic to embedded cells and even it can provide good printability with rapid gelation under calcium ions. However, alginate bioinks do not possess cell-activating ability. To overcome the shortcomings of alginate-based bioinks, a new collagen bioink, which was mixed with human adipose stem cells (hASCs) and crosslinked with a polyphenol (tannic acid), was proposed. The feasibility of the bioink was demonstrated using several in vitro assessments for comparison of the macroscale porous cell-laden collagen/polyphenol structure containing the hASCs with the conventional alginate-based cell-laden structure. The levels of the metabolic activity, including the cell viability and cell proliferation, of the cell-laden collagen structure were significantly higher than those of the control (alginate-based cell-laden structure). The results show that the newly designed bioink and cell-laden structure are potentially new outstanding components for regeneration of various tissues.
authors.affiliation: Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, Republic of Korea.
authors.forename: Myung Gu
authors.initials: MG
authors.lastname: Yeo
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D055119:Elastic Modulus; D004548:Elasticity; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007281:Ink; D051379:Mice; D061848:Optical Imaging; D059808:Polyphenols / Q000494:pharmacology*; D016062:Porosity; D066330:Printing, Three-Dimensional; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D034421:Sus scrofa; D013634:Tannins / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D003432:Cross-Linking Reagents; D059808:Polyphenols; D013634:Tannins; D003094:Collagen