title: Phlebotomine Sand Fly Composition (Diptera: Psychodidae) and Putative Vectors of American Cutaneous Leishmaniasis in Porto Velho Municipality, Western Amazon, Brazil.
abstract: This study evaluated the species composition of sand flies and identified potential vectors of Leishmania Ross species in rural areas of Porto Velho, Rondônia State, Brazil. American cutaneous leishmaniasis (ACL) is one of the gravest threats to public health in this state. Sand flies were collected over the course of 2014 and 2015 using HP light traps. Polymerase chain reaction was performed by targeting the Leishmania mkDNA region. In total, 2,344 sand flies were collected, from which 45 species, nine subgenera, and five species group were identified. The most abundant species were Lutzomyia antunesi (Coutinho) (n = 597, 25.47%), Lutzomyia ubiquitalis (Mangabeira) (n = 496, 21.16%), and Lutzomyia octavioi (Vargas) (n = 199, 8.49%). The greatest diversity occurred in the forest environment where the most abundant species were L. antunesi (n = 588, 25.07%), L. ubiquitalis (n = 493, 21.02%), L. octavioi (n = 199, 8.49%), and Lutzomyia flaviscutellata (Mangabeira) (n = 132, 5.63%). Two pools of L. ubiquitalis were positive for Leishmania DNA, which suggests that L. ubiquitalis is a putative vector of leishmaniasis in the municipality of Porto Velho.
authors.affiliation: Programa de Pós Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, BR 364, Km 9.5, Porto Velho, RO 76801-059, Brazil ( fabiores10@gmail.com; junior.ampj@gmail.com; paulaazevedo.2011@gmail.com ).|Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil ( jmedeiro@gmail.com ).
authors.forename: Fábio
authors.initials: F
authors.lastname: Resadore
authors.affiliation: Programa de Pós Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, BR 364, Km 9.5, Porto Velho, RO 76801-059, Brazil ( fabiores10@gmail.com; junior.ampj@gmail.com; paulaazevedo.2011@gmail.com ).|Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil ( jmedeiro@gmail.com ).
authors.forename: Antônio Marques
authors.initials: AM
authors.lastname: Pereira Júnior
authors.affiliation: Centro de Pesquisas Leônidas e Maria Deane, Rua Terezina, 476, Adrianópolis, Manaus, AM 69057-070, Brazil (lpccbio@gmail.com).
authors.forename: Luís Paulo Costa
authors.initials: LPC
authors.lastname: Carvalho
authors.affiliation: Programa de Pós Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, BR 364, Km 9.5, Porto Velho, RO 76801-059, Brazil ( fabiores10@gmail.com; junior.ampj@gmail.com; paulaazevedo.2011@gmail.com ).|Plataforma de Bioensaios de Malária e Leishmaniose, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil ( carbioni2004@gmail.com ).
authors.forename: Ana Paula de Azevedo
authors.initials: APA
authors.lastname: Dos Santos
authors.affiliation: Plataforma de Bioensaios de Malária e Leishmaniose, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil (carbioni2004@gmail.com).
authors.forename: Carolina Bioni Garcia
authors.initials: CBG
authors.lastname: Teles
authors.affiliation: Laboratório de Entomologia, Fiocruz Rondônia, Rua da Beira, 7671, Porto Velho, RO 76812-245, Brazil (jmedeiro@gmail.com).
authors.forename: Jansen Fernandes
authors.initials: JF
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D058448:Biota; D001938:Brazil; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D065928:Forests; D065948:Grassland; D007303:Insect Vectors / Q000469:parasitology* / Q000502:physiology*; D007891:Leishmania / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction; D011157:Population Dynamics; D011576:Psychodidae / Q000469:parasitology* / Q000502:physiology*
chemical_list: D016054:DNA, Protozoan
keywords: Amazonia; Leishmania; diversity