title: Rotation Measurements of Tethered Cells.
abstract: Direct observation of the rotation of tethered cells using optical microscopy is a simple method to examine dynamics of the bacterial flagellar motor. The rotational speed indicates not only the existence of the rotary motor, but also approximate number of stators that are torque-generating units in a motor. Since "run" and "tumble" of the peritrichous cells as Escherichia coli are regulated by the counterclockwise rotation and the clockwise rotation, respectively, rotational direction of the tethered cell is an important clue to understand the chemotactic system of the cells.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai, Miyagi, 980-8577, Japan. y.inoue@sigma-koki.com.|Sigmakoki Co., Ltd., Tokyo Head Office, 1-19-9 Midori, Sumidaku, Tokyo, 130-0021, Japan. y.inoue@sigma-koki.com.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D002633:Chemotaxis / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005407:Flagella / Q000378:metabolism*; D008954:Models, Biological; D012399:Rotation; D019415:Torque
chemical_list: D029968:Escherichia coli Proteins
keywords: Bacterial flagellar motor; Speed analysis; Switching of the rotational direction; Tethered cell; fliC-sticy mutant