title: Study on synthesis and bioactivity of biotinylated emodin.
abstract: A novel compound biotinylated emodin was synthesized by a two-step acyl chloride method which connects the biotin to emodin with esterification reaction. The product was characterized with ultraviolet-visible spectrophotometry, fourier transform infrared and high-performance liquid chromatography tandem mass spectrometry techniques. Its antibacterial activity against Staphylococcus aureus CMCC 26003 was investigated, and the emodin- and biotinylated emodin-caused antibacterial mechanism was proposed. It was shown that the product was isolated and activity of emodin was remained. These results indicated that our study provides a kind of chemosynthesis method under mild conditions and a strong molecular tool for investigating the emodin-binding protein.
authors.affiliation: College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, 010051, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Hou
authors.affiliation: Tongwei Food and Drug Administration, Gansu, 743300, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Wei
authors.affiliation: College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, 010051, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, 010051, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, 010051, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.affiliation: College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, 010051, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Hong
authors.affiliation: College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, 010051, China. penny__wang@126.com.
authors.forename: Cuiyan
authors.initials: C
authors.lastname: Wang
mesh_terms: D000880:Anthraquinones; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D019921:Biotinylation; D002853:Chromatography, Liquid; D004642:Emodin / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004951:Esterification; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D004642:Emodin
keywords: Bioactivity; Biotinylated emodin; Chemical synthesis; Emodin; Staphylococcus aureus