title: Effect of compatible herbs on the pharmacokinetics of effective components of Panax notoginseng in Fufang Xueshuantong Capsule.
abstract: Fufang Xueshuantong (FXT) is a well-known Chinese herbal formula which has been used to treat cardiovascular and ophthalmic diseases, especially diabetic retinopathy. Panax notoginseng (Burkill) F.H. Chen (PN) is the main herb of FXT, whose major bioactive constituents are ginsenosides. However, the scientific basis of the compatibility of FXT is still ambiguous. The present study investigated the scientific basis of the compatibility of FXT by comparing the pharmacokinetics of marker compounds after oral administrations of PN and FXT. A high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for simultaneous detection of notoginsenoside R1 (NR1), ginsenoside Rg1 (GRg1), and ginsenoside Rb1 (GRb1) in rat plasma. The pharmacokinetic studies of FXT and PN were performed using the established method with the pharmacokinetic parameters being determined by non-compartmental analysis. The results showed that the pharmacokinetic parameters (maximum concentration, area under the curve (AUC0-t), clearance, and mean residence time) of NR1, GRg1, and GRb1 were significantly different after oral administration of FXT (P<0.05) compared with PN. The AUC0-tvalues of GRg1 and GRb1 were 1.7- and 3.4-fold greater, respectively, in FXT than in PN. The compatible herbs of FXT could prolong the retention time and increase the systemic exposure of NR1, GRg1, and GRb1 compared with PN in vivo, providing some scientific basis for the compatibility and clinical use of FXT.
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Pang
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Meng-Yi
authors.initials: MY
authors.lastname: Li
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Min-Ke
authors.initials: MK
authors.lastname: Tang
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Ma
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jian-Mei
authors.initials: JM
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis; D002317:Cardiovascular Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003930:Diabetic Retinopathy / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D036145:Ginsenosides / Q000008:administration & dosage / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D053723:Panax notoginseng / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C519374:Panax notoginseng extract; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1; C072936:notoginsenoside R1
keywords: Fufang Xueshuantong; Panax notoginseng; Pharmacokinetics; Compatibility