title: RpoS Affects Gene Expression in Salmonella enterica serovar Typhi Under Early Hyperosmotic Stress.
abstract: During the infectious procedure of Salmonella enterica serovar Typhi (S. Typhi), Salmonella would suffer from some severe environmental stresses, such as gastric acid stress, enteric hyperosmotic stress, bile acids stress, and oxidative stress. S. Typhi must regulate the expression of numerous genes through the complex regulatory network to adapt strict stresses. RpoS, which encodes sigma factor σs, was reported to be a very important regulator in the maximal survival of enteric pathogens including S. enterica in the stress conditions. However, the role of RpoS in S. Typhi under early hyperosmotic stress is not clear. In this study, we prepared the RpoS-deleted mutant (△RpoS) and compared the growth of △RpoS and wild-type strain. In addition, we analyzed its global transcription profile under early hyperosmotic stress by a microarray. The results showed that △RpoS grew significantly slower than wild-type strain and 24 genes displayed differential expression between the wild-type strain and ΔRpoS upon 30-min exposure in the high osmolarity. Most of these genes are associated with enzymes of metabolism. Taken together, our study firstly demonstrated that RpoS affects gene expression in S. Typhi under early hyperosmotic stress and may impact the growth of bacteria by regulating bacterial metabolic enzymes.
authors.affiliation: Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, 215008, Jiangsu, China. 616463097@qq.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, 215008, Jiangsu, China.
authors.forename: Chaowang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, 215008, Jiangsu, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, 215008, Jiangsu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sui
authors.affiliation: Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, 215008, Jiangsu, China.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, 215008, Jiangsu, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Zhai
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics* / Q000502:physiology; D009994:Osmolar Concentration; D009997:Osmotic Pressure / Q000502:physiology*; D012485:Salmonella typhi / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D012808:Sigma Factor / Q000096:biosynthesis / Q000235:genetics*; D013312:Stress, Physiological / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria