title: Global analysis of H3K27me3 as an epigenetic marker in prostate cancer progression.
abstract: H3K27me3 histone marks shape the inhibition of gene transcription. In prostate cancer, the deregulation of H3K27me3 marks might play a role in prostate tumor progression.
  
  
  
  We investigated genome-wide H3K27me3 histone methylation profile using chromatin immunoprecipitation (ChIP) and 2X400K promoter microarrays to identify differentially-enriched regions in biopsy samples from prostate cancer patients. H3K27me3 marks were assessed in 34 prostate tumors: 11 with Gleason score > 7 (GS > 7), 10 with Gleason score ≤ 7 (GS ≤ 7), and 13 morphologically normal prostate samples.
  
  
  
  Here, H3K27me3 profiling identified an average of 386 enriched-genes on promoter regions in healthy control group versus 545 genes in GS ≤ 7 and 748 genes in GS > 7 group. We then ran a factorial discriminant analysis (FDA) and compared the enriched genes in prostate-tumor biopsies and normal biopsies using ANOVA to identify significantly differentially-enriched genes. The analysis identified ALG5, EXOSC8, CBX1, GRID2, GRIN3B, ING3, MYO1D, NPHP3-AS1, MSH6, FBXO11, SND1, SPATS2, TENM4 and TRA2A genes. These genes are possibly associated with prostate cancer. Notably, the H3K27me3 histone mark emerged as a novel regulatory mechanism in poor-prognosis prostate cancer.
  
  
  
  Our findings point to epigenetic mark H3K27me3 as an important event in prostate carcinogenesis and progression. The results reported here provide new molecular insights into the pathogenesis of prostate cancer.
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Ngollo
authors.affiliation: University Blaise Pascal, Institut Pascal UMR 6602 CNRS/UBP, 24 Avenue des Landais, Aubière, France.
authors.forename: Andre
authors.initials: A
authors.lastname: Lebert
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Daures
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Judes
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.
authors.forename: Khaldoun
authors.initials: K
authors.lastname: Rifai
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.
authors.forename: Lucas
authors.initials: L
authors.lastname: Dubois
authors.affiliation: Department of Biopathology, Gabriel Montpied Hospital, 58 rue Montalembert, 63000, Clermont-Ferrand, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Kemeny
authors.affiliation: INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.|Department of Biopathology, Centre Jean Perrin, 58 rue Montalembert, 63000, Clermont-Ferrand, France.
authors.forename: Frederique
authors.initials: F
authors.lastname: Penault-Llorca
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.
authors.forename: Yves-Jean
authors.initials: YJ
authors.lastname: Bignon
authors.affiliation: INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France.|Department of Urology, Gabriel Montpied Hospital, 58 rue Montalembert, 63000, Clermont-Ferrand, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Guy
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin - CBRV, 28 place Henri Dunant, BP 38, 63001, Clermont-Ferrand, France. dominique.gallon-bernard@clermont.unicancer.fr.|INSERM U 1240, IMOST, 58 rue Montalembert-BP184, 63005, Clermont-Ferrand, France. dominique.gallon-bernard@clermont.unicancer.fr.
authors.forename: Dominique
authors.initials: D
authors.lastname: Bernard-Gallon
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000090266:Chromobox Protein Homolog 5; D019175:DNA Methylation; D016002:Discriminant Analysis; D018450:Disease Progression; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011379:Prognosis; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: C000715593:CBX1 protein, human; D006657:Histones; D000090266:Chromobox Protein Homolog 5
keywords: Cancer; ChIP-on-chip; Epigenetics; H3K27me3; Histone methylation; Prostate