title: SNHG1 lncRNA negatively regulates miR-199a-3p to enhance CDK7 expression and promote cell proliferation in prostate cancer.
abstract: Long noncoding RNAs (lncRNAs) have been reported to play vital roles in the development of human cancers, but our understandings of most lncRNAs in cancers are still limited. Recently, accumlating evidences have showed that many RNA transcripts could function as competing endogenous RNAs (ceRNAs) by competitively binding common microRNAs. In this study, we demonstrated that a lncRNA, Small Nucleolar RNA Host Gene 1 (SNHG1), as a ceRNA for miR-199a-3p, played a critical role in prostate cancer cell proliferation. We found that SNHG1 was aberrantly up-regulated in prostate carcinoma tissues; while, miR-199a-3p was abnormally down-regulated. The level of SNHG1 in prostate cancer was significantly negatively correlated with that of miR-199a-3p. Our data indicated that SNHG1 could interact with miR-199a-3p and inhibit the activity of miR-199a-3p in prostate cancer cells. In addition, miR-199a-3p could target the 3' UTR of CDK7 and suppress CDK7 expression. More importantly, SNHG1 increased CDK7 expression by competitively binding miR-199a-3p, and then promoted cell proliferation and cell cycle progression in prostate cancer. Taken together, these findings elucidated a novel mechanism of prostate cancer progression. Thus, SNHG1 might serve as a potential target for prostate cancer therapies.
authors.affiliation: Department of Operation, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pubilc Health, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: People's Hospital of Luxian, Luzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Urology, Chengdu Chengfei Hospital, Chengdu, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Operation, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Operation, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
authors.forename: Lilan
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Operation, The Affiliated Hospital of Southwest Medical University, Luzhou, China. Electronic address: 2518494951@qq.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000235:genetics*; D014407:Tumor Cells, Cultured; D000095043:Cyclin-Dependent Kinase-Activating Kinase
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding; C000613724:long non-coding RNA SNHG1, human; C531408:mirn199 microRNA, human; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase; C000722416:CDK7 protein, human
keywords: CDK7; Proliferation; Prostate cancer; SNHG1; miR-199a-3p