title: Effect of Fenton pre-oxidation on mobilization of nutrients and efficient subsequent bioremediation of crude oil-contaminated soil.
abstract: Fenton pre-oxidation and a subsequent bioremediation phase of 80 days were used to investigate the importance of matching concentration of residual indigenous bacteria and nutrient levels on subsequent bioremediation of crude oil. Experiments were performed using either high (>107.7 ± 0.2CFU/g soil) or low (<105.9 ± 0.1CFU/g soil) concentrations of bacteria and three different nutrient levels: enough (C/N > 9.8), moderate (C/N:5-9.8), and lacking nutrient level (C/N < 5) conditions. Weak Fenton pre-oxidation (225 mM H2O2and 2.9 mM Fe2+) resulted in highly matching between nutrient level and the population of residual indigenous bacteria. Up to 53% of total petroleum hydrocarbon (TPH) and 58% of main hydrocarbon (C15C25, during the first 10 days) were removed from the soil. Under matching conditions, the activity of indigenous bacteria and nutrient mobilization were enhanced, promoting the bioremediation of crude oil. In addition, the biodegradation of long chain molecules (C26C30) required a high level of NH4+-N.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China. Electronic address: xujinlan@xauat.edu.cn.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China.
authors.forename: Fanxing
authors.initials: F
authors.lastname: Kong
authors.affiliation: Huaqing College, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Song
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Cao
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China.
authors.forename: Tinglin
authors.initials: T
authors.lastname: Huang
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Cui
mesh_terms: D001673:Biodegradation, Environmental; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: C045076:Fenton's reagent; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Bioremediation; Crude oil-contaminated soil; Fenton pre-oxidation; Nutrient mobilization; Residual indigenous bacteria