title: Impact of different nitrogen sources on the growth of Arthrospira sp. PCC 8005 under batch and continuous cultivation - A biochemical, transcriptomic and proteomic profile.
abstract: The aim of the present study was to evaluate the effects of varying concentrations of different nitrogen sources (individually or in combination) on the biochemical, transcriptomic and proteomic profiles of Arthrospira sp. PCC 8005 under batch and continuous modes. In batch mode, while ammonium showed a repressive effect on nitrate-assimilation pathway of the cyanobacteria; better growth and nutrient uptake rate were observed in presence of urea than nitrate. The inhibitory effect of ammonium was further confirmed by the continuous photobioreactor study wherein the nutrient feed was transiently replaced from nitrate to ammonium (28mM turbiostat regime). The changes in lipid, exopolysaccharide, transcriptomic and proteomic profiles of cyanobacteria on transition from nitrate to ammonium indicated at an onset of nutrient stress.
authors.affiliation: Department of Proteomic and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 20, B-7000 Mons, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Deschoenmaeker
authors.affiliation: Department of Proteomic and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 20, B-7000 Mons, Belgium.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bayon-Vicente
authors.affiliation: Department of Proteomic and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 20, B-7000 Mons, Belgium.
authors.forename: Neha
authors.initials: N
authors.lastname: Sachdeva
authors.affiliation: Laboratory Aquatic Biology, KU Leuven Campus Kortrijk, E. Sabbelaan 53, 8500 Kortrijk, Belgium.
authors.forename: Orily
authors.initials: O
authors.lastname: Depraetere
authors.affiliation: Biotechnology Unit, Materia Nova, Rue des Foudriers 1, B-7822 Ghislenghien, Belgium.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Cabrera Pino
authors.affiliation: Department of Proteomic and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 20, B-7000 Mons, Belgium.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Leroy
authors.affiliation: Laboratory Aquatic Biology, KU Leuven Campus Kortrijk, E. Sabbelaan 53, 8500 Kortrijk, Belgium.
authors.forename: Koenraad
authors.initials: K
authors.lastname: Muylaert
authors.affiliation: Department of Proteomic and Microbiology, Research Institute for Biosciences, University of Mons, Place du Parc 20, B-7000 Mons, Belgium. Electronic address: Ruddy.Wattiez@umons.ac.be.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
mesh_terms: D000458:Cyanobacteria; D015964:Gene Expression Regulation, Bacterial; D009566:Nitrates; D009584:Nitrogen; D040901:Proteomics; D053545:Spirulina
chemical_list: D009566:Nitrates; D009584:Nitrogen
keywords: Arthrospira sp. PCC 8005; Nitrogen metabolism; Omics; Photobioreactor; Waste recycling