title: Engineering and expressing circular RNAs via tRNA splicing.
abstract: Circular (circ)RNAs have recently become a subject of great biologic interest. It is now clear that they represent a diverse and abundant class of RNAs with regulated expression and evolutionarily conserved functions. There are several mechanisms by which RNA circularization can occur in vivo. Here, we focus on the biogenesis of tRNA intronic circular RNAs (tricRNAs) in archaea and animals, and we detail their use as research tools for orthogonal, directed circRNA expression in vivo.
authors.affiliation: a Curriculum in Genetics and Molecular Biology , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|b Integrative Program for Biological and Genome Sciences , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Noto
authors.affiliation: a Curriculum in Genetics and Molecular Biology , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|b Integrative Program for Biological and Genome Sciences , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: Casey A
authors.initials: CA
authors.lastname: Schmidt
authors.affiliation: a Curriculum in Genetics and Molecular Biology , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|b Integrative Program for Biological and Genome Sciences , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|c Lineberger Comprehensive Cancer Center , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|d Department of Biology , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|e Department of Genetics , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: A Gregory
authors.identifier: 0000-0002-6406-0630
authors.initials: AG
authors.lastname: Matera
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics* / Q000378:metabolism; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D005091:Exons; D005818:Genetic Engineering / Q000379:methods*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D051379:Mice; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D022821:RNA Splice Sites; D012326:RNA Splicing; D000079962:RNA, Circular; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D052157:Aptamers, Nucleotide; D012322:RNA Precursors; D022821:RNA Splice Sites; D000079962:RNA, Circular; D012313:RNA; D012343:RNA, Transfer
keywords: Back-splicing; RNA aptamers; circRNA; ectopic gene expression; tRNA processing; tricRNA