title: Effect of sodium salt of Butrin, a novel compound isolated from Butea monosperma flowers on suppressing the expression of SIRT1 and Aurora B kinase-mediated apoptosis in colorectal cancer cells.
abstract: The infrequent manifestation of SIRT1 and Aurora B kinase has shown to play a pivotal role in colorectal cancer (CRC) progression by regulating Wnt signaling pathway. The present study investigates the signaling events that regulate the modulation of SIRT1 and Aurora B kinase expression and it's mediated cell proliferation in SW480 human primary adenocarcinoma CRC cells using Butea monosperma floral compounds (BMFC). In this, cell viability, mitochondrial mediated apoptosis, cell cycle arrest and inhibition of Wnt pathway were examined. Interestingly, the active novel compound, sodium salt of butrin, from BMFC significantly enhances the apoptosis activity, where SIRT1 and Aurora B kinase were ectopically overexpressed in CRC cells. Moreover, mRNA and protein expressions analysis indicates that the expression of GSK-3β, β-catenin, cyclin D1, pAKT, TGF-3β, SIRT1 and Aurora B kinase were down regulated in BMFC treated cells. These findings provide valuable information that the active BMFC having great impact on SIRT1 and Aurora B kinase mediated Wnt signaling down regulation in SW480 CRC cells.
authors.affiliation: Department of Biomedical Science, School of Basic Medical Science, Bharathidasan University, Tiruchirappalli, 620 024 Tamilnadu, India.
authors.forename: Boopathi
authors.initials: B
authors.lastname: Subramaniyan
authors.affiliation: Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biomedical Science, School of Basic Medical Science, Bharathidasan University, Tiruchirappalli, 620 024 Tamilnadu, India. Electronic address: mathan@bdu.ac.in.
authors.forename: Ganeshan
authors.initials: G
authors.lastname: Mathan
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D064107:Aurora Kinase B / Q000378:metabolism*; D031290:Butea / Q000737:chemistry*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D056564:Sirtuin 1 / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D060449:Wnt Signaling Pathway / Q000187:drug effects; D051176:beta Catenin / Q000378:metabolism
chemical_list: D005419:Flavonoids; D012333:RNA, Messenger; D051176:beta Catenin; D019938:Cyclin D1; C049386:butrin; D012964:Sodium; D064107:Aurora Kinase B; D000071679:Glycogen Synthase Kinase 3 beta; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: AURKB and apoptosis; CRC; Curcmin; SIRT1; Sodium salt of butrin