title: Dicer1 dysfunction promotes stemness and aggression in endometrial carcinoma.
abstract: Endometrial carcinoma is one of the most common gynecological malignancies, but the molecular events involved in the development and progression of endometrial carcinoma remain unclear. Dicer1 and cancer stem cells play important roles in cell motility and survival. This study investigated the role of the let-7 family and Dicer1 in the stemness of endometrial carcinoma cells. We profiled Dicer1 expression in clinical samples and explored its relationship with stem cell-associated markers and clinical parameters. We showed that Dicer1 dysfunction leads to the enrichment of tumor stemness features and tumor aggression both in vitro and in vivo. We also identified the mechanism related to this potential tumor-predisposing phenotype: loss of Dicer1 induced abnormal expression of the let-7 family, which comprises well-known tumor suppressors, thus regulating stemness in endometrial carcinoma cells.
authors.affiliation: 1 Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Health Hospital, Tongji University School of Medicine, Shanghai, China.|2 Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: 3 Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Fei-Zhou
authors.initials: FZ
authors.lastname: Jiang
authors.affiliation: 1 Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Health Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Tong
authors.affiliation: 2 Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jie-Qi
authors.initials: JQ
authors.lastname: Ke
authors.affiliation: 1 Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Health Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Li
authors.affiliation: 2 Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: 2 Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Yan
authors.affiliation: 2 Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Fang-Yuan
authors.initials: FY
authors.lastname: Wang
authors.affiliation: 1 Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Health Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wan
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases / Q000502:physiology*; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000502:physiology; D043244:Ribonuclease III / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000502:physiology
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; C487931:mirnlet7 microRNA, human; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
keywords: Dicer1; Endometrial carcinoma; let-7; stemness