title: Phylogenetic analysis and survey of Apis cerana strain of Sacbrood virus (AcSBV) in Taiwan suggests a recent introduction.
abstract: The Sacbrood virus (SBV) is widely distributed in European honey bees, Apis mellifera. AcSBV, a distinct SBV strain in Asian honey bees (A. cerana) causes larva death before pupation and often depopulates colonies, leading to collapse. It is the most severe disease in A. cerana beekeeping. AcSBV infects A. cerana in most natural habitats, yet occurrences were not reported in Taiwan before 2015 and were not a concern for local beekeepers. However, in 2016, A. cerana beekeepers in central Taiwan reported SBV-like symptoms. We screened samples of larvae using RT-PCR and surveyed asymptomatic apiaries in north Taiwan. Phylogenetic analyses suggested that AcSBV isolates from central Taiwan were introduced; all isolates had high similarity in sequences to AcSBV genomes identified in mainland China, Vietnam, and Korea and distinct differences to SBV sequence identified in Taiwan. The overall prevalence in symptomatic colonies was low. No latent infections were detected in asymptomatic colonies. The AcSBV epizootic may not yet have reached its highest potential.
authors.affiliation: College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Wei-Fone
authors.initials: WF
authors.lastname: Huang
authors.affiliation: College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Shahid
authors.initials: S
authors.lastname: Mehmood
authors.affiliation: College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Shaokang
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Ilan 26047, Taiwan, ROC.
authors.forename: Yue-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Ilan 26047, Taiwan, ROC.
authors.forename: Chong-Yu
authors.initials: CY
authors.lastname: Ko
authors.affiliation: College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: susongkun@zju.edu.cn.
authors.forename: Songkun
authors.initials: S
authors.lastname: Su
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D013624:Taiwan
keywords: AcSBV; Apis cerana; Chinese Sacbrood; SBV; Sacbrood