title: Binding of poly(amidoamine), carbosilane, phosphorus and hybrid dendrimers to thrombin-Constants and mechanisms.
abstract: Thrombin is an essential part of the blood coagulation system; it is a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, and catalyzes many other coagulation-related reactions. Absorption at its surface of small nanoparticles can completely change the biological properties of thrombin. We have analyzed the influence on thrombin of 3 different kinds of small nanoparticles: dendrimers (phosphorus-based, carbosilane based and polyamidoamine) and 2 hybrid systems containing carbosilane, viologen and phosphorus dendritic scaffolds in one single molecule, bearing different flexibility, size and surface charge. There was significant alteration in the rigidity of the rigid dendrimers in contrast to flexible dendrimers. These differences in their action are important in understanding interactions taking place at a bio-nanointerface.
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus. Electronic address: shcharbin@gmail.com.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pedziwiatr-Werbicka
authors.affiliation: Department of Biophysics, Faculty of Physics, Belarussian State University, Minsk, Belarus.
authors.forename: Aliaksandra
authors.initials: A
authors.lastname: Vcherashniaya
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Janaszewska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marcinkowska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Goska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert-Maculewicz
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus.
authors.forename: Viktar
authors.initials: V
authors.lastname: Abashkin
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus.
authors.forename: Aliaksei
authors.initials: A
authors.lastname: Ihnatsyeu-Kachan
authors.affiliation: Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Spain; Networking Research Center on Bioengineering, Biomatrials and Nanomedicine (CIBER-BBN), Spain.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.affiliation: Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Spain; Networking Research Center on Bioengineering, Biomatrials and Nanomedicine (CIBER-BBN), Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Ortega
authors.affiliation: Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Spain; Networking Research Center on Bioengineering, Biomatrials and Nanomedicine (CIBER-BBN), Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Gomez-Ramirez
authors.affiliation: Laboratorie de Chimie de Coordination, CNRS, Toulouse, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Majoral
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D001665:Binding Sites; D001777:Blood Coagulation; D050091:Dendrimers / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D010758:Phosphorus / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012821:Silanes / Q000737:chemistry*; D012996:Solutions; D055672:Static Electricity; D013917:Thrombin / Q000737:chemistry*
chemical_list: D050091:Dendrimers; C577210:PAMAM G2.5 polyamidoamine; D012821:Silanes; D012996:Solutions; C504072:carbosilane; D010758:Phosphorus; D013917:Thrombin
keywords: Dendrimer; Interactions; Mechanisms; Thrombin