title: Antimicrobial Resistance and Molecular Characterization ofStaphylococcus aureusCausing Childhood Pneumonia in Shanghai.
abstract: Staphylococcus aureusor methicillin-resistantStaphylococcus aureus(MRSA) is a major pathogen causing pneumonia among children. To estimate the prevalence and molecular properties ofS. aureusin children pneumonia in Shanghai, China, 107 hospitalized children withS. aureuspneumonia from two children's hospitals from January 2014 through June 2015 were studied.S. aureusisolates from the respiratory specimens were characterized by antimicrobial susceptibility,agrtyping, toxin genes, multilocus sequence typing (MLST),spa, and SCCmec typing. Fifty-eight (54.2%, 58/107) were MSSA (methicillin-susceptibleStaphylococcus aureus) and 49 (45.8%, 49/107) were MRSA. No isolates were found resistant to teicoplanin, sulfamethoxazole/trimethoprim, rifampicin, quinupristin/dalfopristin, linezolid, or vancomycin. However, these isolates showed high resistant rates to erythromycin, fosfomycin-trometamol and clindamycin. TheagrI(87/107, 81.3%) was the most commonagrallele, followed byagrIII(10/107, 9.3%),agrII(9/107, 8.4%), andagrIV(1/107, 0.9%). Sixpvl-positive isolates (3 MRSA and 3 MSSA) and 7 isolates of livestock associated clone ST398 (4 MRSA, 3 MSSA) were identified. CC59 was found in 35 isolates (33 MRSA and 2 MSSA), constituting majority of MRSA (33/49, 67.35%). The dominant CC were CC59 (32.7%), CC188 (13.1%), CC7 (12.1%) and CC398 (9.3%) while t172 (16.8%), t189 (12.1%), t437 (9.3%), and t091 (9.3%) were the most commonspatypes. In conclusion, more particular concern should appeal to ST59-SCCmecIV-t172/t437 as it is the most common epidemic clone causing pneumonia among children in Shanghai.
authors.affiliation: Faculty of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Gu
authors.affiliation: Department of Immunology and Microbiology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Guo
authors.affiliation: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Yu-Xing
authors.initials: YX
authors.lastname: Ni
authors.affiliation: Department of Immunology and Microbiology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
authors.forename: Li-Zhong
authors.initials: LZ
authors.lastname: Han
keywords: MRSA; Staphylococcus aureus; antimicrobial resistance; childhood pneumonia; molecular characterization