title: Establishment of reference values for complete blood count and blood gases in cynomolgus monkeys (Macaca fascicularis).
abstract: Cynomolgus monkeys are closely related to humans phylogenetically, and this has resulted in their widespread use as a preclinical model. Hematological data with regard to these monkeys are thus important. Although reference values for blood components and sex hormones have been established for cynomolgus monkeys, those for arterial blood gases have not. The arterial blood gases quickly reflect respiratory and circulatory dynamics, and are thus useful for animal management and safe general anesthesia and surgical operations. Furthermore, since O2is transported by RBC, CBC and blood gases are closely related. The present study aimed to establish reference values for arterial blood gases and CBC in cynomolgus monkeys over a wide age range. Blood gases and CBC of arterial blood, collected from 41 female and 21 male anesthetized monkeys, were measured. Age correlated with RBC, HGB and HCT in the CBC. Values differed significantly between males and females in pCO2, CO2concentration, MCV and MCH. The pH of blood was equivalent to that of humans and pCO2was more stable, whereas MCV and MCH were lower than those in humans. Erythrocytes were smaller and less pigmented than in other Macaca species. Several relationships between gender and age, and blood gases and CBC were identified in cynomolgus monkeys. In conclusion, these reference values will be useful as markers for veterinary applications and in the care and maintenance of these animals.
authors.affiliation: Nihon University, Collage of Bioresource Science, Kanagawa 252-0880, Japan.|Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki 305-0843, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Nakayama
authors.affiliation: Nihon University, Collage of Bioresource Science, Kanagawa 252-0880, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koie
authors.affiliation: Nihon University, Collage of Bioresource Science, Kanagawa 252-0880, Japan.
authors.forename: Kiichi
authors.initials: K
authors.lastname: Kanayama
authors.affiliation: The Corporation for Production and Research of Laboratory Primates, Ibaraki 305-0003, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Katakai
authors.affiliation: Nihon University, Collage of Bioresource Science, Kanagawa 252-0880, Japan.|Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki 305-0843, Japan.
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Ito-Fujishiro
authors.affiliation: Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki 305-0843, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sankai
authors.affiliation: Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki 305-0843, Japan.|Mie University Graduate School of Medicine, Department of Molecular and Experimental Medicine, Mie 514-8507, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yasutomi
authors.affiliation: Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki 305-0843, Japan.
authors.forename: Naohide
authors.initials: N
authors.lastname: Ageyama
mesh_terms: D000375:Aging / Q000097:blood; D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary*; D001784:Blood Gas Analysis / Q000662:veterinary*; D005260:Female; D008252:Macaca fascicularis / Q000097:blood*; D008297:Male; D012016:Reference Values
keywords: aging; arterial blood gas; complete blood count; cynomolgus monkey; nonhuman primate