title: Functional bacterial and archaeal diversity revealed by 16S rRNA gene pyrosequencing during potato starch processing wastewater treatment in an UASB.
abstract: Microbial community structure of sludge sampled from an UASB treating potato starch processing wastewater (PSPW) was investigated. Operational taxonomic units revealed at 97% sequence identity tolerance was 2922, 2869 and 3919 for bottom, middle and top sections of the reactor, respectively. Overall abundant phylum observed within the UASB was low-G+C-Gram-positive bacteria affiliated to Firmicutes (26.01%) followed by Chloroflexi (16.70%), Proteobacteria (12.71%), Cloacimonetes (10.72%), Bacteroidetes (7.87%), Synergistetes (9.02%) and Euryarchaeota (8.82%). Whiles Firmicutes had dominated the bottom and top section by 34.01% and 28.64%, respectively, middle section was predominantly Euryarchaeota (24.32%) with major dominance in methanogens affiliated to genus Methanosaeta. The results demonstrated substantial stratification of the microbial community structure along the reactor height with various functional bacterial groups which subsequently allowed degradation of organics in PSPW in sequential mode. The findings herein would provide guidance for optimizing the anaerobic process and operation of the UASB.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China.
authors.forename: Philip
authors.initials: P
authors.lastname: Antwi
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China. Electronic address: ljz6677@163.com.
authors.forename: Jianzheng
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Management, Harbin Institute of Technology, 92 West Dazhi Street, Nan Gang District, Harbin 150001, PR China.
authors.forename: Portia
authors.initials: P
authors.lastname: Opoku Boadi
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China.
authors.forename: En
authors.initials: E
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Geography, University of North Carolina, 237 Graham Building, 1009 Spring Garden St, Greensboro, NC 27412, USA.
authors.forename: Frederick
authors.initials: F
authors.lastname: Ayivi
mesh_terms: D001105:Archaea / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D019149:Bioreactors / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012722:Sewage / Q000382:microbiology; D011198:Solanum tuberosum; D013213:Starch; D062065:Wastewater
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013213:Starch; D062065:Wastewater
keywords: Anaerobic process; Functional bacteria; Microbial community structure; Operational taxonomic units; Potato starch processing wastewater