title: Technological and Theoretical Aspects for Testing Electroporation on Liposomes.
abstract: Recently, the use of nanometer liposomes as nanocarriers in drug delivery systems mediated by nanoelectroporation has been proposed. This technique takes advantage of the possibility of simultaneously electroporating liposomes and cell membrane with 10-nanosecond pulsed electric fields(nsPEF)facilitating the release of the drug from the liposomes and at the same time its uptake by the cells. In this paper the design and characterization of a 10nsPEFexposure system is presented, for liposomes electroporation purposes. The design and the characterization of the applicator have been carried out choosing an electroporation cuvette with 1 mm gap between the electrodes. The structure efficiency has been evaluated at different experimental conditions by changing the solution conductivity from 0.25 to 1.6 S/m. With the aim to analyze the influence of device performances on the liposomes electroporation, microdosimetric simulations have been performed considering liposomes of 200 and 400 nm of dimension with different inner and outer conductivity (from 0.05 to 1.6 S/m) in order to identify the voltage needed for their poration.
authors.affiliation: Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 00161 Rome, Italy; Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome "La Sapienza", 00184 Rome, Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Denzi
authors.affiliation: Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome "La Sapienza", 00184 Rome, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Della Valle
authors.affiliation: Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome "La Sapienza", 00184 Rome, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Esposito
authors.affiliation: Vectorology and Antitumor Therapies, UMR 8203, CNRS, Univ. Paris-Sud, Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France.
authors.forename: Lluis M
authors.initials: LM
authors.lastname: Mir
authors.affiliation: Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome "La Sapienza", 00184 Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Apollonio
authors.affiliation: Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome "La Sapienza", 00184 Rome, Italy.
authors.forename: Micaela
authors.identifier: 0000-0002-7494-2696
authors.initials: M
authors.lastname: Liberti
mesh_terms: D002462:Cell Membrane / Q000187:drug effects; D016503:Drug Delivery Systems; D004574:Electromagnetic Fields; D018274:Electroporation / Q000379:methods*; D006801:Humans; D019169:Jurkat Cells; D008081:Liposomes / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D008081:Liposomes