title: Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata).
abstract: The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sparus aurata), is a self-limiting condition characterized by the appearance of hypertrophied fibroblasts (named lymphocysts) in the connective tissue of fish, primarily in the skin and fins. The causative agent of the disease is the Lymphocystis disease virus (LCDV), a member of the Iridoviridae family. In the present study, LCDV genome and transcripts were detected by real-time PCR in caudal fin, as well as in several internal organs, such as intestine, liver, spleen, kidney and brain, from asymptomatic, diseased and recovered gilthead seabream juveniles. These results indicate that the LCDV has a broad range tissue tropism, and can establish a systemic infection, even in subclinically infected fish. As showed by in situ hybridization, the permissive cells for LCDV infection seem to be fibroblasts, hepatocytes and cells of the mononuclear phagocyte system. Histopathological alterations associated with LCD were observed in all the organs analysed, including necrotic changes in liver and kidney, inflammatory response in the intestine submucosa or brain haemorrhage, although lymphocysts were only detected in the dermis of the caudal fin. Nevertheless, these histological changes were reverted in recovered animals.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario Teatinos, Malaga, Spain.
authors.forename: Estefania J
authors.initials: EJ
authors.lastname: Valverde
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario Teatinos, Malaga, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Borrego
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, CSIC, Puerto Real, Cádiz, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Sarasquete
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, CSIC, Puerto Real, Cádiz, Spain.
authors.forename: Juan B
authors.initials: JB
authors.lastname: Ortiz-Delgado
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario Teatinos, Malaga, Spain. dcastro@uma.es.
authors.forename: Dolores
authors.identifier: 0000-0002-1589-3820
authors.initials: D
authors.lastname: Castro
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D017403:In Situ Hybridization / Q000662:veterinary; D007497:Iridoviridae / Q000235:genetics / Q000502:physiology*; D021541:Sea Bream / Q000821:virology*; D019562:Viral Load / Q000662:veterinary; D014779:Virus Replication / Q000502:physiology
chemical_list: D004279:DNA, Viral