title: Genome analysis of Heliothis virescens ascovirus 3h isolated from China.
abstract: No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h (HvAV-3h) using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G+C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames (ORFs) along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between HvAV-3h and three other HvAV-3a strains and a distant relationship with Spodoptera frugiperda ascovirus 1a (SfAV-1a), Trichoplusia ni ascovirus 6a (TnAV-6a), and Diadromus pulchellus ascovirus 4a (DpAV-4a). Among the 185 potential genes encoded by the genome, 44 core genes were found in all the sequenced ascoviruses. In addition, 25 genes were found to be conserved in all ascoviruses except DpAV-4a. In the HvAV-3h genome, 24 baculovirus repeat ORFs (bros) were present, and the typical homologous repeat regions (hrs) were absent. This study supplies information important for understanding the conservation and functions of ascovirus genes as well as the variety of ascoviral genomes.
authors.affiliation: State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China.|Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Institute of Virology, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China.
authors.forename: Dian-Hai
authors.initials: DH
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China.
authors.forename: Manli
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Microbiology, 32 Pearson Hall, Miami University, Oxford, Ohio, 45056, USA.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China. huzh@wh.iov.cn.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D029223:Ascoviridae / Q000235:genetics* / Q000302:isolation & purification*; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D016679:Genome, Viral; D007915:Lepidoptera / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral
keywords: Heliothis virescens ascovirus 3h (HvAV-3h); bro genes; genome organization; phylogenetic relationship