title: Genotyping ofIL-4-590 (C>T) Gene in Iraqi Asthma Patients.
abstract: This study is the first investigation in Iraq dealing with genotyping ofIL-4-590 (C>T) gene, especially in Iraqi patients with asthma. We studied forty-eight blood samples collected from patients with asthma and compared with age-matched 25 healthy individuals as controls. The polymorphism results ofIL-4-590 (C>T) gene by using amplification refractory mutation system (ARMS-PCR) showed the presence ofCandTalleles and three genotypes (CC, CT, and TT). Interestingly the frequency ofCallele and CC genotype was higher in patients with asthma in comparison with the same allele and genotype in control (P1 × 10-6). This increase was associated with an increased risk factor of asthma (odds ratio [OR] 9.21; 95% confidence interval [CI] 3.58-23.71). Genotypes analysis by using Hardy-Weinberg distribution showed no significant differences between patients with asthma and healthy subjects. In conclusion, the increasing risk of asthma was associated withCallele and the CC genotype and these are revealed as etiological fraction with risk by having this disease, while theTallele percentage ratio in controls was higher when it is compared with asthma patients suggesting that these alleles have a protective effect (preventive fraction).
authors.affiliation: Department of Biology, College of Education for Pure Science-Ibn Al-Haytham, University of Baghdad, Baghdad, Iraq.
authors.forename: Ihsan A
authors.identifier: 0000-0002-2991-2113
authors.initials: IA
authors.lastname: Hussein
authors.affiliation: Department of Biology, College of Education for Pure Science-Ibn Al-Haytham, University of Baghdad, Baghdad, Iraq.
authors.forename: Saddam H
authors.initials: SH
authors.lastname: Jaber
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005838:Genotype; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D007493:Iraq; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C508598:IL4 protein, human; D015847:Interleukin-4