title: Differential Proteomic Profiles ofPleurotus ostreatusin Response to Lignocellulosic Components Provide Insights into Divergent Adaptive Mechanisms.
abstract: Pleurotus ostreatusis a white rot fungus that grows on lignocellulosic biomass by metabolizing the main constituents. Extracellular enzymes play a key role in this process. During the hydrolysis of lignocellulose, potentially toxic molecules are released from lignin, and the molecules are derived from hemicellulose or cellulose that trigger various responses in fungus, thereby influencing mycelial growth. In order to characterize the mechanism underlying the response ofP. ostreatusto lignin, we conducted a comparative proteomic analysis ofP. ostreatusgrown on different lignocellulose substrates. In this work, the mycelium proteome ofP. ostreatusgrown in liquid minimal medium with lignin, xylan, and carboxymethyl cellulose (CMC) was analyzed using the complementary two-dimensional gel electrophoresis (2-DE) approach; 115 proteins were identified, most of which were classified into five types according to their function. Proteins with an antioxidant function that play a role in the stress response were upregulated in response to lignin. Most proteins involving in carbohydrate and energy metabolism were less abundant in lignin. Xylan and CMC may enhanced the process of carbohydrate metabolism by regulating the level of expression of various carbohydrate metabolism-related proteins. The change of protein expression level was related to the adaptability ofP. ostreatusto lignocellulose. These findings provide novel insights into the mechanisms underlying the response of white-rot fungus to lignocellulose.
authors.affiliation: Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural UniversityKunming, China.
authors.forename: Qiuyun
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Fuying
authors.initials: F
authors.lastname: Ma
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University Kunming, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University Kunming, China.
authors.forename: Chengyun
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
keywords: Pleurotus ostreatus; fungal adaptability; lignocellulose; proteomics; white-rot fungus