title: Electrophoretic profiles of lipopolysaccharides from Rhizobium strains nodulating Pisum sativum do not reflect phylogenetic relationships between these strains.
abstract: Rhizobia that nodulate peas comprise a heterogeneous group of bacteria. The aim of this study was to investigate the relationship between phylogeny and electrophoretic and hydroxy fatty acid lipopolysaccharide (LPS) profiles of pea microsymbionts. Based on amplified fragment length polymorphism (AFLP) fingerprinting data, the pea microsymbionts were grouped into two clusters distinguished at 58% similarity level. Based on the concatenated 16S rRNA, recA, and atpD housekeeping gene data, the microsymbionts appeared to be most closely related to Rhizobium leguminosarum biovars viciae and trifolii. Applying cluster analysis to their LPS electrophoretic profiles, the strains were assigned to two major groups with different banding patterns. All hydroxy fatty acids common to R. leguminosarum and R. etli were detected in each examined strain. Differences in the proportions of 3- to ω-1 hydroxy fatty acids allowed us to distinguish two groups of strains. This classification did not overlap with one based on LPS electrophoretic profiles. No clear correlation was apparent between the genetic traits and LPS profiles of the pea nodule isolates.
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland. jolanta.kutkowska@poczta.umcs.lublin.pl.
authors.forename: Jolanta
authors.identifier: http://orcid.org/0000-0002-3307-591X
authors.initials: J
authors.lastname: Kutkowska
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marek-Kozaczuk
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Wielbo
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Wójcik
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Urbanik-Sypniewska
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis*; D008565:Membrane Proteins / Q000235:genetics; D018532:Pisum sativum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D016961:Rhizobium leguminosarum / Q000145:classification / Q000235:genetics / Q000378:metabolism; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases
keywords: 16S rRNA; AFLP fingerprinting; Fatty acids; Lipopolysaccharides; Pisum sativum; Rhizobium leguminosarum