title: Transcriptome, antioxidant enzyme activity and histopathology analysis of hepatopancreas from the white shrimp Litopenaeus vannamei fed with aflatoxin B1(AFB1).
abstract: Aflatoxin produced by Aspergillus flavus or Aspergillus parasiticus fungi during grain and feed processing and storage. Aflatoxins cause severe health problems reducing the yield and profitability of shrimp cultures. We sought to understand the interaction between shrimp immunity and aflatoxin B1 (AFB1), analyzing transcriptome expression, antioxidant enzyme activity, and histological features of the hepatopancreas of shrimp fed with AFB1. From over 4 million high-quality reads, de novo unigene assembly produced 103,644 fully annotated genes. A total of 1024 genes were differentially expressed in shrimp fed with AFB1, being involved in functions, such as peroxidase metabolism, signal transduction, transcriptional control, apoptosis, proteolysis, endocytosis, and cell adhesion and cell junction. Upon AFB1 challenge, there were severe histological alterations in shrimp hepatopancreas. AFB1 challenge increased the activity of several antioxidant enzymes. Our data contribute to improve the current understanding of host-AFB1 interaction, providing an abundant source for identification of novel genes.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; College of Fishery Science, Tianjin Agriculture University/Tianjin Key Laboratory of Aquaculture, Tianjin 300384, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Keyong
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Mengqiang
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Fishery Science, Tianjin Agriculture University/Tianjin Key Laboratory of Aquaculture, Tianjin 300384, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Cancan
authors.initials: C
authors.lastname: Qi
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: wangbaojie@qdio.ac.cn.
authors.forename: Baojie
authors.initials: B
authors.lastname: Wang
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D001231:Aspergillus flavus / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics; D004705:Endocytosis / Q000235:genetics; D005786:Gene Expression Regulation; D043143:Hepatopancreas / Q000473:pathology / Q000502:physiology*; D033561:Penaeidae / Q000502:physiology*; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D059748:Proteolysis; D015398:Signal Transduction / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D016604:Aflatoxin B1; D009195:Peroxidase
keywords: Aflatoxin B1; Enzyme activity; Hepatopancreas; Histopathology; Litopenaeus vannamei; Transcriptome