title: An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints.
abstract: DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints-touch DNA "sandwiched" between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post-amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri-Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7-100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases.
authors.affiliation: Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284.
authors.forename: April D
authors.initials: AD
authors.lastname: Solomon
authors.affiliation: Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284.
authors.forename: Madison E
authors.initials: ME
authors.lastname: Hytinen
authors.affiliation: Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284.
authors.forename: Aryn M
authors.initials: AM
authors.lastname: McClain
authors.affiliation: Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284.
authors.forename: Marilyn T
authors.initials: MT
authors.lastname: Miller
authors.affiliation: Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284.
authors.forename: Tracey
authors.initials: T
authors.lastname: Dawson Cruz
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D003878:Dermatoglyphics; D006801:Humans; D018895:Microsatellite Repeats; D013048:Specimen Handling / Q000379:methods*; D014110:Touch; D057188:Workflow
chemical_list: D004247:DNA
keywords: DNA typing; adhesive tape; archived latent fingerprints; forensic science; paper substrate; short tandem repeat typing; touch DNA