title: Circulating Aneuploid Cells Detected in the Blood of Patients with Infectious Lung Diseases.
abstract: The identification of circulating tumor cells (CTCs) is clinically important for diagnosing cancer. We have previously developed a size-based filtration platform followed by epithelial cell adhesion molecule immunofluorescence staining for detecting CTCs. To characterize CTCs independently of cell surface protein expression, we incorporated a chromosomal fluorescencein situhybridization (FISH) assay to detect abnormal copy numbers of chromosomes in cells collected from peripheral blood samples by the size-based filtration platform. Aneuploid cells were detected in the peripheral blood of patients with lung cancer. Unexpectedly, aneuploid cells were also detected in the control group, which consisted of peripheral blood samples from patients with benign lung diseases, such as empyema necessitatis and non-tuberculous mycobacterial lung disease. These findings suggest that chromosomal abnormalities are observed not only in tumor cells, but also in benign infectious diseases. Thus, our findings present new considerations and bring into light the possibility of false positives when using FISH for cancer diagnosis.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Sungkyunkwan University School of Medicine.
authors.forename: Hongsun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Sungkyunkwan University School of Medicine.
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine.
authors.forename: Chung-Hee
authors.initials: CH
authors.lastname: Sonn
authors.affiliation: Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Sungkyunkwan University School of Medicine.
authors.forename: Yul
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine.
authors.forename: Jinseon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Sungkyunkwan University School of Medicine; Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine.
authors.forename: Jhingook
authors.initials: J
authors.lastname: Kim
keywords: Circulating neoplastic cells; Fluorescent in situ hybridization; Lung neoplasms