title: Resistin polymorphims, plasma resistin levels and obesity in Tunisian volunteers.
abstract: BACKGROUND
  Adipose tissue is an important endocrine organ that secretes a number of adipokines, like Resistin (RETN); it's an adipocytes-secreted cytokine and has been proposed as a link between obesity and diabetes. Many resistin gene polymorphisms were described and their implication in obesity was controversial. This study was to investigate the prevalence of single nucleotide polymorphisms (SNPs) in RETN gene 420C/G; 44G/A; 62G/A; 394C/G and 299 G/A and their association with Resistin level and obesity in Tunisian volunteers.
  
  
  METHODS
  We recruited 169 nonobese (mean age=42.16-14.26 years; mean body mass index [BMI]=24.51-3.69 kg/m2) and 160 obese (mean age=47.86-11.17 years; mean BMI=36-4.78 kg/m2). Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism. Anthropometric parameters, lipid levels, Glycemia and insulinemia were measured, BMI was calculated and insulinresistance was evaluated with the homeostasis model assessment insulin resistance (HOMA-IR) and resistin level was measured by ELISA. Statistical analyses were performed by SPSS19.0.
  
  
  RESULTS
  After adjustment for confounding parameters; the Odds Ratio (OR) of obesity associated with mutated genotypes at 420C/G compared with normal genotype was as: OR=2.17; 95% CI [1.28-3.68], P=.004. The serum Resistin levels present no significant association with all RETN polymorphisms and it was significantly associated with BMI (P=.047). In our haplotype analysis, one haplotype seems to be protective and one other seems to be the highest risk to obesity.
  
  
  CONCLUSIONS
  The 420 C/G Polymorphism were associated with obesity and Leptin concentration in our population.
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Nesrine
authors.identifier: http://orcid.org/0000-0002-5610-1068
authors.initials: N
authors.lastname: Zayani
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Haithem
authors.initials: H
authors.lastname: Hamdouni
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Boumaiza
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Ons
authors.initials: O
authors.lastname: Achour
authors.affiliation: Laboratory of Biochemistry and Toxicology, Monastir's University Hospital, Monastir, Tunisia.
authors.forename: Fadoua
authors.initials: F
authors.lastname: Neffati
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Asma
authors.initials: A
authors.lastname: Omezzine
authors.affiliation: Laboratory of Biochemistry and Toxicology, Monastir's University Hospital, Monastir, Tunisia.
authors.forename: Mohamed Fadhel
authors.initials: MF
authors.lastname: Najjar
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Bouslama
mesh_terms: D000328:Adult; D005260:Female; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012372:ROC Curve; D052243:Resistin / Q000097:blood* / Q000235:genetics*; D012307:Risk Factors; D014416:Tunisia / Q000453:epidemiology
chemical_list: C499624:RETN protein, human; D052243:Resistin
keywords: levels; obesity; polymorphisms; resistin; tunisian volunteers