title: Effect of Fabp1/Scp-2/Scp-x Ablation on Whole Body and Hepatic Phenotype of Phytol-Fed Male Mice.
abstract: Liver fatty acid binding protein (Fabp1) and sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x) genes encode proteins that enhance hepatic uptake, cytosolic transport, and peroxisomal oxidation of toxic branched-chain fatty acids derived from dietary phytol. Since male wild-type (WT) mice express markedly higher levels of these proteins than females, the impact of ablating both genes (TKO) was examined in phytol-fed males. In WT males, high phytol diet alone had little impact on whole body weight and did not alter the proportion of lean tissue mass (LTM) versus fat tissue mass (FTM). TKO conferred on dietary phytol the ability to induce weight loss as well as reduce liver weight, FTM, and even more so LTM. Concomitantly TKO induced hepatic lipid accumulation, preferentially threefold increased phospholipid (PL) at the expense of decreased triacylglycerol (TG) and total cholesterol. Increased PL was associated with upregulation of membrane fatty acid transport/translocase proteins (FATP 2,4), cytosolic fatty acid/fatty acyl-CoA binding proteins (FABP2, ACBP), and the rate limiting enzyme in PL synthesis (Gpam). Decreased TG and cholesterol levels were not attributable to altered levels in respective synthetic enzymes or nuclear receptors. These data suggest that the higher level of Fabp1 and Scp2/Scpx gene products in WT males was protective against deleterious effects of dietary phytol, but TKO significantly exacerbated phytol effects in males.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TVMC, College Station, TX, 77843-4467, USA.
authors.forename: Danilo
authors.initials: D
authors.lastname: Landrock
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TVMC, College Station, TX, 77843-4467, USA.
authors.forename: Sherrelle
authors.initials: S
authors.lastname: Milligan
authors.affiliation: Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4466, USA.
authors.forename: Gregory G
authors.initials: GG
authors.lastname: Martin
authors.affiliation: Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4466, USA.
authors.forename: Avery L
authors.initials: AL
authors.lastname: McIntosh
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TVMC, College Station, TX, 77843-4467, USA.
authors.forename: Kerstin K
authors.initials: KK
authors.lastname: Landrock
authors.affiliation: Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4466, USA.
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Schroeder
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TVMC, College Station, TX, 77843-4467, USA. akier@cvm.tamu.edu.
authors.forename: Ann B
authors.initials: AB
authors.lastname: Kier
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D002352:Carrier Proteins / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D055786:Gene Knockout Techniques; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010836:Phytol / Q000008:administration & dosage* / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; C493320:Fabp1 protein, mouse; C493405:Fabp2 protein, mouse; D050556:Fatty Acid-Binding Proteins; D010743:Phospholipids; C072087:sterol carrier proteins; D010836:Phytol
keywords: Branched-fatty acid; FABP1; Obesity; Phytol