title: Genotypic analysis ofTropheryma whippleifrom patients with Whipple disease in the Americas.
abstract: Tropheryma whipplei, the agent of Whipple disease, causes a rare bacterial disease that may be fatal if not treated. The classical form of the disease includes diarrhoea, weight loss, arthritis, endocarditis and neurological manifestations. Genotyping studies done in Europe, Africa and Asia showed high genetic diversity with no correlation between genotypes and clinical features, but contributed to a better understanding of the epidemiology of the disease. More than 70 genotypes have been described. No similar assessment ofT. whippleiin the USA and the Caribbean has been performed. In this study, we describe genetic analysis of DNA from histopathological samples obtained from 30 patients from the Americas with Whipple disease and compare the genotypes with those previously identified. Complete genotypes were obtained from 18 patients (60%). Only 4 genotypes were previously described, and 14 were newly reported, confirming the diversity ofT. whippleistrains.
authors.affiliation: Infectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Dominique C
authors.identifier: http://orcid.org/0000-0001-8454-685X
authors.initials: DC
authors.lastname: Rollin
authors.affiliation: Rickettsial Zoonoses Branch, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Paddock
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Bobbi S
authors.initials: BS
authors.lastname: Pritt
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Cunningham
authors.affiliation: Infectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Amy M
authors.initials: AM
authors.lastname: Denison
mesh_terms: D000328:Adult; D000368:Aged; D000569:Americas; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D054851:Tropheryma / Q000235:genetics*; D008061:Whipple Disease / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: CLINICAL INFECTIOUS DISEASES; IMMUNOHISTOCHEMISTRY; MICROBIAL GENETICS