title: Fluoxetine effects on periodontogenesis: histomorphometrical and immunohistochemical analyses in rats.
abstract: OBJECTIVE
  This study aimed to assess the effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament during pregnancy and lactation in rat pups.
  
  
  METHODS
  Twelve pregnant rats of Wistar lineage were divided into four study groups. In the control group, 0.9% sodium chloride solution was administered orally, throughout the entire period of the 21 days of pregnancy (CG group) and in the CGL group, it was administrated during pregnancy and lactation (from day 1 of pregnancy to the 21st day after birth). Fluoxetine was administered orally at the dose of 20 mg/kg in a group treated during pregnancy only (FG group), and during pregnancy and lactation (FGL group). Histometrical, histochemical and immunohistochemical analysis of the maxillary first molar periodontium region of the 24 rat pups was made under light microscopy, and periodontal ligament collagen was qualitatively evaluated under a polarizing light microscope.
  
  
  RESULTS
  The quantity of fibroblasts (p=0.006), osteoblasts (p=0.027) and cementoblasts (p=0.001) was reduced in pups from the rats that received fluoxetine during pregnancy and lactation. No alterations were seen in the collagen fibers.
  
  
  CONCLUSIONS
  These findings suggest that periodontal tissue may be sensitive to fluoxetine, and its interference in reducing periodontal cells depends on exposure time during lactation.
authors.affiliation: Universidade Federal de Pernambuco, Faculdade de Odontologia, Recife, PE, Brasil.
authors.forename: Luciana Silva
authors.initials: LS
authors.lastname: Regueira
authors.affiliation: Universidade Federal de Pernambuco, Faculdade de Odontologia, Recife, PE, Brasil.
authors.forename: Priscylla Gonçalves Correia Leite de
authors.initials: PG
authors.lastname: Marcelos
authors.affiliation: Universidade Federal de Pernambuco, Faculdade de Odontologia, Recife, PE, Brasil.
authors.forename: Isabela Maria
authors.initials: IM
authors.lastname: Santiago-Jaegger
authors.affiliation: Universidade Federal de Pernambuco, Faculdade de Odontologia, Departamento de Clínica e Odontologia Preventiva, Seção de Patologia Oral Recife, PE, Brasil.
authors.forename: Danyel Elias da Cruz
authors.initials: DE
authors.lastname: Perez
authors.affiliation: Universidade Federal de Pernambuco, Faculdade de Odontologia, Departamento de Morfologia e Fisiologia Animal, Recife, PE.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Evêncio
authors.affiliation: Universidade Federal de Pernambuco, Faculdade de Odontologia, Departamento de Histologia e Embriologia, Recife, PE, Brasil.
authors.forename: Liriane
authors.initials: L
authors.lastname: Baratella-Evêncio
mesh_terms: D000539:Alveolar Process / Q000187:drug effects / Q000196:embryology; D000818:Animals; D005260:Female; D024022:Fibrillar Collagens / Q000032:analysis; D005473:Fluoxetine / Q000494:pharmacology*; D007150:Immunohistochemistry; D007774:Lactation; D008297:Male; D018811:Maternal Exposure; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D011247:Pregnancy; D011897:Random Allocation; D017208:Rats, Wistar; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D024022:Fibrillar Collagens; D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors