title: Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
abstract: In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides. The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes. By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable. Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties. Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis. Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model. Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China. Electronic address: yuxw@sjtu.edu.cn.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, PR China. Electronic address: yfzhou@sjtu.edu.cn.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002356:Cartilage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects; D020219:Chondrogenesis; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D009842:Oligopeptides; D010368:Pectins / Q000737:chemistry*; D013552:Swine; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D010368:Pectins; D006820:Hyaluronic Acid
keywords: Cartilage tissue engineering; Hyaluronic acid; Injectable hydrogel; Oligopeptide G(4)RGDS; Pectin