title: An easy method for bacterial expression and purification of wild-type and mutant superoxide dismutase 1 (SOD1).
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE), Sigmund-Freud-Straße 27, 53127 Bonn, Germany.
authors.forename: Babila J
authors.initials: BJ
authors.lastname: Tachu
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE), Sigmund-Freud-Straße 27, 53127 Bonn, Germany.
authors.forename: Katharina A
authors.initials: KA
authors.lastname: Wüsten
authors.affiliation: Centre for Prions and Protein Folding Diseases & Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Garza
authors.affiliation: Centre for Prions and Protein Folding Diseases & Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Holger
authors.initials: H
authors.lastname: Wille
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE), Sigmund-Freud-Straße 27, 53127 Bonn, Germany. Electronic address: erdem@dzne.de.
authors.forename: Gültekin
authors.initials: G
authors.lastname: Tamgüney
mesh_terms: D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D066329:Protein Aggregates; D000072105:Superoxide Dismutase-1 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D066329:Protein Aggregates; D000072105:Superoxide Dismutase-1
keywords: Aggregation; Amyloid; Amyotrophic lateral sclerosis (ALS); Enzyme activity; Oligomers; SOD1