title: Gene Expression Profiles of Human Phosphotyrosine Phosphatases Consequent to Th1 Polarisation and Effector Function.
abstract: Phosphotyrosine phosphatases (PTPs) constitute a complex family of enzymes that control the balance of intracellular phosphorylation levels to allow cell responses while avoiding the development of diseases. Despite the relevance of CD4 T cell polarisation and effector function in human autoimmune diseases, the expression profile of PTPs during T helper polarisation and restimulation at inflammatory sites has not been assessed. Here, a systematic analysis of the expression profile of PTPs has been carried out during Th1-polarising conditions and upon PKC activation and intracellular raise of Ca2+in effector cells. Changes in gene expression levels suggest a previously nonnoted regulatory role of several PTPs in Th1 polarisation and effector function. A substantial change in the spatial compartmentalisation of ERK during T cell responses is proposed based on changes in the dose of cytoplasmic and nuclear MAPK phosphatases. Our study also suggests a regulatory role of autoimmune-related PTPs in controlling T helper polarisation in humans. We expect that those PTPs that regulate T helper polarisation will constitute potential targets for intervening CD4 T cell immune responses in order to generate new therapies for the treatment of autoimmune diseases.
authors.affiliation: Department of Microbiology I (Immunology), School of Medicine, Complutense University and "12 de Octubre" Health Research Institute, Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Castro-Sánchez
authors.affiliation: Department of Microbiology I (Immunology), School of Medicine, Complutense University and "12 de Octubre" Health Research Institute, Madrid, Spain.
authors.forename: Rocio
authors.initials: R
authors.lastname: Ramirez-Munoz
authors.affiliation: Department of Microbiology I (Immunology), School of Medicine, Complutense University and "12 de Octubre" Health Research Institute, Madrid, Spain.
authors.forename: Pedro
authors.identifier: 0000-0003-3799-8823
authors.initials: P
authors.lastname: Roda-Navarro
mesh_terms: D001327:Autoimmune Diseases / Q000276:immunology* / Q000628:therapy; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002454:Cell Differentiation; D006801:Humans; D008213:Lymphocyte Activation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D015398:Signal Transduction; D018417:Th1 Cells / Q000276:immunology* / Q000502:physiology*; D059467:Transcriptome
chemical_list: D017027:Protein Tyrosine Phosphatases; D002118:Calcium