title: High Density Individually Addressable Nanowire Arrays Record Intracellular Activity from Primary Rodent and Human Stem Cell Derived Neurons.
abstract: We report a new hybrid integration scheme that offers for the first time a nanowire-on-lead approach, which enables independent electrical addressability, is scalable, and has superior spatial resolution in vertical nanowire arrays. The fabrication of these nanowire arrays is demonstrated to be scalable down to submicrometer site-to-site spacing and can be combined with standard integrated circuit fabrication technologies. We utilize these arrays to perform electrophysiological recordings from mouse and rat primary neurons and human induced pluripotent stem cell (hiPSC)-derived neurons, which revealed high signal-to-noise ratios and sensitivity to subthreshold postsynaptic potentials (PSPs). We measured electrical activity from rodent neurons from 8 days in vitro (DIV) to 14 DIV and from hiPSC-derived neurons at 6 weeks in vitro post culture with signal amplitudes up to 99 mV. Overall, our platform paves the way for longitudinal electrophysiological experiments on synaptic activity in human iPSC based disease models of neuronal networks, critical for understanding the mechanisms of neurological diseases and for developing drugs to treat them.
authors.forename: Ren
authors.initials: R
authors.lastname: Liu
authors.forename: Renjie
authors.identifier: 0000-0002-3145-6882
authors.initials: R
authors.lastname: Chen
authors.forename: Ahmed T
authors.initials: AT
authors.lastname: Elthakeb
authors.forename: Sang Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute , 10901 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Sandy
authors.initials: S
authors.lastname: Hinckley
authors.forename: Massoud L
authors.initials: ML
authors.lastname: Khraiche
authors.forename: John
authors.initials: J
authors.lastname: Scott
authors.affiliation: Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute , 10901 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Deborah
authors.initials: D
authors.lastname: Pre
authors.forename: Yoontae
authors.initials: Y
authors.lastname: Hwang
authors.forename: Atsunori
authors.initials: A
authors.lastname: Tanaka
authors.forename: Yun Goo
authors.initials: YG
authors.lastname: Ro
authors.forename: Albert K
authors.initials: AK
authors.lastname: Matsushita
authors.affiliation: Division of Physics and Applied Physics, Nanyang Technological University , 21 Nanyang Link, Singapore 637371, Singapore.
authors.forename: Xing
authors.initials: X
authors.lastname: Dai
authors.affiliation: Division of Physics and Applied Physics, Nanyang Technological University , 21 Nanyang Link, Singapore 637371, Singapore.
authors.forename: Cesare
authors.initials: C
authors.lastname: Soci
authors.affiliation: Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute , 10901 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Steven
authors.initials: S
authors.lastname: Biesmans
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories , Albuquerque, New Mexico 87185, United States.
authors.forename: Anthony
authors.initials: A
authors.lastname: James
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories , Albuquerque, New Mexico 87185, United States.
authors.forename: John
authors.initials: J
authors.lastname: Nogan
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories , Albuquerque, New Mexico 87185, United States.
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Jungjohann
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories , Albuquerque, New Mexico 87185, United States.
authors.forename: Douglas V
authors.initials: DV
authors.lastname: Pete
authors.affiliation: Center for Integrated Nanotechnologies, Sandia National Laboratories , Albuquerque, New Mexico 87185, United States.
authors.forename: Denise B
authors.initials: DB
authors.lastname: Webb
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute , 10901 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Anne G
authors.initials: AG
authors.lastname: Bang
authors.forename: Shadi A
authors.identifier: 0000-0002-1756-1774
authors.initials: SA
authors.lastname: Dayeh
mesh_terms: D000200:Action Potentials; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D056656:Lab-On-A-Chip Devices; D051379:Mice; D008839:Microelectrodes; D053770:Nanowires / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010316:Particle Size; D051381:Rats
keywords: Nanowire; drug-screening; extracellular; intracellular; neuron; subthreshold