title: Comparative bacterial community analysis in relatively pristine and anthropogenically influenced mangrove ecosystems on the Red Sea.
abstract: Mangrove habitats are ecologically important ecosystems that are under severe pressure worldwide because of environmental changes and human activities. In this study, 16S rRNA gene amplicon deep-sequencing was used to compare bacterial communities in Red Sea mangrove ecosystems at anthropogenically influenced coastal sites with those at a relatively pristine island site. In total, 32 phyla were identified from the mangrove rhizospheres, with Proteobacteria predominating at each of the studied sites; however, the relative abundance was significantly decreased at the coastal sites (Mastorah, MG-MS; Ar-Rayis, MG-AR) compared with the pristine island site near Dhahban (MG-DBI). The phyla Actinobacteria, Firmicutes, Acidobacteria, Chloroflexi, Spirochetes, and Planctomycetes were present at a relative abundance of >1% at the MG-MS and MG-AR sites, but their concentration was <1% at the MG-DBI site. A total of 1659 operational taxonomic units (OTUs) were identified at the species level, and approximately 945 OTUs were shared across the different sampling sites. Multivariate principal coordinate data analysis separated the MG-DBI site from the MG-AR and MG-MS cluster. Specific bacterial taxa were enriched at each location, and in particular, the genera Pseudoalteromonas and Cobetia were predominantly identified in the MG-DBI site compared with the anthropogenically influenced coastal sites.
authors.affiliation: a Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.|b Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Riaz
authors.initials: R
authors.lastname: Ullah
authors.affiliation: a Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yasir
authors.affiliation: a Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.|b Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.affiliation: a Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Fehmida
authors.initials: F
authors.lastname: Bibi
authors.affiliation: a Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Sayed Sartaj
authors.initials: SS
authors.lastname: Sohrab
authors.affiliation: c Department of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Ansari
authors.affiliation: b Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Fahad
authors.initials: F
authors.lastname: Al-Abbasi
authors.affiliation: d Marine Biology Department, Faculty of Marine Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Abdulmohsin A
authors.initials: AA
authors.lastname: Al-Sofyani
authors.affiliation: e Department of Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ihsanullah
authors.initials: I
authors.lastname: Daur
authors.affiliation: f Department of Applied Biology, Dong-A University, Busan 49315, Republic of Korea.
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Lee
authors.affiliation: a Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.|g Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Esam I
authors.initials: EI
authors.lastname: Azhar
mesh_terms: D061271:Acidobacteria / Q000235:genetics; D032407:Avicennia; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D017753:Ecosystem; D000068536:Bacillota; D019156:Indian Ocean; D020560:Proteobacteria / Q000145:classification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D014871:Water Microbiology; D053833:Wetlands
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Mer Rouge; Red Sea; bacterial community; communauté bactérienne; mangroves; metagenomic; métagénomique; rhizosphere; rhizosphère