title: A contribution to pharmaceutical biology of freshwater sponges.
abstract: In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50value of 5.01 ± 0.21 μg/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50value 2.42 ± 0.13 μg/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50values 3.68 ± 0.19 and 3.14 ± 0.09 μg/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin.
authors.affiliation: a Faculty of Biology , University of Belgrade , Belgrade , Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Talevska
authors.affiliation: b Department of Life Sciences , Institute for Multidisciplinary Research - IMSI, University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.affiliation: c Oncology Institute of Vojvodina, University of Novi Sad , Sremska Kamenica , Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Kojic
authors.affiliation: a Faculty of Biology , University of Belgrade , Belgrade , Serbia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Beric
authors.affiliation: a Faculty of Biology , University of Belgrade , Belgrade , Serbia.
authors.forename: Slavisa
authors.initials: S
authors.lastname: Stankovic
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005618:Fresh Water; D006801:Humans; D020128:Inhibitory Concentration 50; D011161:Porifera / Q000737:chemistry*; D014020:Tissue Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D016166:Free Radical Scavengers; D014020:Tissue Extracts; D004317:Doxorubicin
keywords: A-549; Lake Ohrid; Malawispongiidae; Ochridaspongia rotunda; anti-DPPH radical activity; crude extract; no genotoxicity