title: λ-Carrageenan improves the antitumor effect of dendritic cellbased vaccine.
abstract: In this study, we investigated the effect of λ-carrageenan on the maturation and function of dendritic cells (DCs) and its adjuvant effect on DC-based vaccine. We found that λ-carrageenan dose-dependently decreased the endocytosis of DCs, promoted DC maturation and increased cytokine production through TLR4 mediated signaling pathway. λ-carrageenan treatment also enhanced the ability of DCs in the stimulating allogenic splenocyte proliferation. In TC-1 tumor mouse model, HPV peptides pulsed λ-carrageenan-DC (HPV-CGN-DC) significantly inhibited tumor growth compared with control group. The frequencies of CD4+ and CD8+ T cells in spleens of tumor mice and their activation status were significantly increased in HPV-CGN-DC group, but the frequencies of natural regulatory T cells and CD11b+Gr-1+ cells were significantly decreased. Further, HPV-CGN-DC induced strong CD8+ T cell responses, which are negatively correlated with tumor volumes. The results suggested that λ-carrageenan promoted DC maturation through TLR4 signaling pathway and could be used as the adjuvant in DC-based vaccines.
authors.affiliation: Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.|College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.|XinJiang DingJu Biotech CO., LTD, Urumqi 830000, China.
authors.forename: Jinyao
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Adila
authors.initials: A
authors.lastname: Aipire
authors.affiliation: College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Hongge
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Bayin Guoleng Vocational and Technical College, Korla 841000, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Guo
authors.affiliation: Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Li
authors.affiliation: Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yang
authors.affiliation: Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002351:Carrageenan / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D004705:Endocytosis / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015398:Signal Transduction / Q000187:drug effects; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D016896:Treatment Outcome; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D019496:Cancer Vaccines; D016207:Cytokines; D051197:Toll-Like Receptor 4; D002351:Carrageenan
keywords: TLR4; cancer immunotherapy; cellular responses; dendritic cell-based vaccine; λ-carrageenan