title: miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2.
abstract: Many reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutical target for many diseases, even of human cancers; however, there are no reports on the role of miR-597 in human cancers. In the present study, by detecting mRNA expression with qRT-PCR, compared with the adjacent normal tissues we found that miR-597 was significantly downregulated in breast cancer tissues. By using the MTT assay, the cell wound-healing assay and the cell invasion assay, we demonstrated that miR-597 mimics were able to suppress breast cancer cell proliferation, migration and invasion. Additionally, with flow cytometry, we found that mir-597 influenced the growth of breast cancer cells through regulating the G1-S phase transition. Furthermore, we identified one binding site for miR-597 at the 3'UTR of the FOSL2 gene, using bioinformatics methods and the luciferase reporter assay, it was confirmed that FOSL2 was a direct target of miR-597. Moreover, overexpression of FOSL2 in MDA-MB‑231 and SK-BR-3 cells can block the vast majority of the miR-597 roles, suggesting that miR-597 acts as a tumor suppressor in breast cancer cells by the downregulation of FOSL2. Additionally, we also found a negative correlation between the expression of FOSL2 and miR-597 in the tumor samples. This new regulatory mechanism in breast cancer may provide another method for diagnosis and therapy.
authors.affiliation: The Research Center for Immunology, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Jiangtu
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Central Laboratory, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Mai
authors.affiliation: Department of Clinical Laboratory, Maternal and Child Health Care of Zaozhuang, Zaozhuang, Shandong 277102, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Tai Zhou Hospital of Zhejiang Province, Taizhou, Zhejiang 317099, P.R China.
authors.forename: Linyi
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Tai Zhou Hospital of Zhejiang Province, Taizhou, Zhejiang 317099, P.R China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: The Research Center for Immunology, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Central Laboratory, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Pan
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D051921:Fos-Related Antigen-2 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*
chemical_list: C497872:FOSL2 protein, human; D051921:Fos-Related Antigen-2; C000619454:MIRN-598 microRNA, human; D035683:MicroRNAs