title: Gallium-modified chitosan/poly(acrylic acid) bilayer coatings for improved titanium implant performances.
abstract: A gallium-modified chitosan/poly(acrylic acid) bilayer was obtained by electrochemical techniques on titanium to reduce orthopaedic and/or dental implants failure. The bilayer in vitro antibacterial properties and biocompatibility were evaluated against Escherichia coli and Pseudomonas aeruginosa and on MG63 osteoblast-like cells, respectively. Gallium loading into the bilayer was carefully tuned by the electrochemical deposition time to ensure the best balance between antibacterial activity and cytocompatibility. The 30min deposition time was able to reduce in vitro the viable cell counts of E. coli and P. aeruginosa of 2 and 3 log cfu/sheet, respectively. Our results evidenced that the developed antibacterial coating did not considerably alter the mechanical flexural properties of titanium substrates and, in addition, influenced positively MG63 adhesion and proliferation. Therefore, the gallium-modified chitosan/poly(acrylic acid) bilayer can be exploited as a promising titanium coating to limit bacterial adhesion and proliferation, while maintaining osseointegrative potential.
authors.affiliation: Dept. of Chemistry, University of Bari Aldo Moro, Bari, Italy.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Bonifacio
authors.affiliation: Jaber Innovation srl, Roma, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Cometa
authors.affiliation: Dept. of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Dicarlo
authors.affiliation: Institute of Sciences of Food Production (ISPA-CNR), National Research Council of Italy, Bari.
authors.forename: Federico
authors.initials: F
authors.lastname: Baruzzi
authors.affiliation: Institute of Sciences of Food Production (ISPA-CNR), National Research Council of Italy, Bari.
authors.forename: Silvia
authors.initials: S
authors.lastname: de Candia
authors.affiliation: Institute of Polymers, Composites and Biomaterials (IPCB-CNR), National Research Council of Italy, Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gloria
authors.affiliation: Institute of Nanotechnology (NANOTEC-CNR), National Research Council of Italy, Bari, Italy.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Giangregorio
authors.affiliation: Dept. of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Mattioli-Belmonte
authors.affiliation: Dept. of Chemistry, University of Bari Aldo Moro, Bari, Italy. Electronic address: elvira.degiglio@uniba.it.
authors.forename: Elvira
authors.initials: E
authors.lastname: De Giglio
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000900:Anti-Bacterial Agents; D001422:Bacterial Adhesion; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004926:Escherichia coli; D005708:Gallium / Q000737:chemistry*; D019736:Prostheses and Implants; D011550:Pseudomonas aeruginosa; D013499:Surface Properties; D014025:Titanium
chemical_list: D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; C006903:carbopol 940; D048271:Chitosan; D005708:Gallium; D014025:Titanium
keywords: Ammonium peroxydisulfate (PubChem CID: 62648); Antibacterial agents; Biocompatibility; Chitosan hydrochloride (PubChem SID: 295370713); Chitosan-based bilayer; Electrochemical deposition; Gallium; Gallium (III) nitrate hydrate (PubChem CID: 11550823); Poly(acrylic acid) (PubChem CID: 6581); Titanium; Ttanium (PubChem CID: 23963)