title: Beyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis.
abstract: The emergence of novel binding proteins or antibody mimetics capable of binding to ligand analytes in a manner analogous to that of the antigen-antibody interaction has spurred increased interest in the biotechnology and bioanalytical communities. The goal is to produce antibody mimetics designed to outperform antibodies with regard to binding affinities, cellular and tumor penetration, large-scale production, and temperature and pH stability. The generation of antibody mimetics with tailored characteristics involves the identification of a naturally occurring protein scaffold as a template that binds to a desired ligand. This scaffold is then engineered to create a superior binder by first creating a library that is then subjected to a series of selection steps. Antibody mimetics have been successfully used in the development of binding assays for the detection of analytes in biological samples, as well as in separation methods, cancer therapy, targeted drug delivery, and in vivo imaging. This review describes recent advances in the field of antibody mimetics and their applications in bioanalytical chemistry, specifically in diagnostics and other analytical methods.
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33136; email: sdaunert@med.miami.edu.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33136; email: sdaunert@med.miami.edu.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33136; email: sdaunert@med.miami.edu.
authors.forename: Emre
authors.initials: E
authors.lastname: Dikici
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33136; email: sdaunert@med.miami.edu.
authors.forename: Sapna K
authors.initials: SK
authors.lastname: Deo
authors.affiliation: Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33136; email: sdaunert@med.miami.edu.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Daunert
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D052158:Aptamers, Peptide / Q000737:chemistry / Q000378:metabolism; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D011485:Protein Binding; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000906:Antibodies; D052158:Aptamers, Peptide; D011993:Recombinant Fusion Proteins
keywords: antibodies; antibody mimetics; biosensors; diagnostics; molecular imaging; protein scaffolds