title: MicroRNA-27b functions as a new inhibitor of ovarian cancer-mediated vasculogenic mimicry through suppression ofVE-cadherinexpression.
abstract: Aggressive cancer cells gain robust tumor vascular mimicry (VM) capability that promotes tumor growth and metastasis.VE-cadherinis aberrantly overexpressed in vasculogenic cancer cells and regarded as a master gene of tumor VM. Although microRNAs (miRNAs) play an important role in modulating tumor angiogenesis and cancer metastasis, the miRNA that targetsVE-cadherinexpression in cancer cells to inhibit tumor cell-mediated VM is enigmatic. In this study, we found that miR-27b levels are negatively co-related toVE-cadherinexpression in ovarian cancer cells and tumor cell-mediated VM, and demonstrated that miR-27b could bind to the 3'-untranslated region (3'UTR) ofVE-cadherinmRNA. Overexpression of miR-27b in aggressive ovarian cancer cell lines Hey1B and ES2 significantly diminished intracellularVE-cadherinexpression; convincingly, the inhibitory effect of miR-27b could be reversed by miR-27b specific inhibitor. Intriguingly, miR-27b not only effectively suppressed ovarian cancer cell migration and invasion, but also markedly inhibited formation of ovarian cancer cell-mediated capillary-like structures in vitro and suppressed generation of functional tumor blood vessels in mice. Together, our study suggests that miR-27b functions as a new inhibitor of ovarian cancer cell-mediated VM through suppression ofVE-cadherinexpression, providing a new potential drug candidate for antitumor VM and anti-ovarian cancer therapy.
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, 2011 Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province, and Chinese Ministry of Science and Technology, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, 2011 Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province, and Chinese Ministry of Science and Technology, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Lv
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, 2011 Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province, and Chinese Ministry of Science and Technology, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, 2011 Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province, and Chinese Ministry of Science and Technology, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, 2011 Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province, and Chinese Ministry of Science and Technology, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Cao
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D015820:Cadherins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000098:blood supply* / Q000235:genetics*; D000099152:Cadherin 5
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; D015820:Cadherins; D035683:MicroRNAs; D000099152:Cadherin 5; C531388:MIRN27 microRNA, human
keywords: VE-cadherin; miR-27b; ovarian cancer; vasculogenic mimicry