title: NMR probing and visualization of correlated structural fluctuations in intrinsically disordered proteins.
abstract: A novel statistical analysis of paramagnetic relaxation enhancement (PRE) and paramagnetic relaxation interference (PRI) based nuclear magnetic resonance (NMR) data is proposed based on the computation of correlation matrices. The technique is demonstrated with an example of the intrinsically disordered proteins (IDPs) osteopontin (OPN) and brain acid soluble protein 1 (BASP1). The correlation analysis visualizes in detail the subtleties of conformational averaging in IDPs and highlights the presence of correlated structural fluctuations of individual sub-domains in IDPs.
authors.affiliation: Département de Chimie, Ecole Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Laboratoire des Biomolécules (LBM), 24 rue Lhomond, 75005 Paris, France. kurzbach@ens.fr.
authors.forename: Dennis
authors.initials: D
authors.lastname: Kurzbach
authors.forename: Andreas
authors.initials: A
authors.lastname: Beier
authors.forename: Agathe
authors.initials: A
authors.lastname: Vanas
authors.forename: Andrea G
authors.initials: AG
authors.lastname: Flamm
authors.forename: Gerald
authors.initials: G
authors.lastname: Platzer
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Schwarz
authors.forename: Robert
authors.initials: R
authors.lastname: Konrat
mesh_terms: D006801:Humans; D064267:Intrinsically Disordered Proteins / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D053495:Osteopontin / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: C104089:BASP1 protein, human; D064267:Intrinsically Disordered Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D053495:Osteopontin