title: Histone Modifications Pattern Associated With a State of Mesenchymal Stem Cell Cultures Derived From Amniotic Fluid of Normal and Fetus-Affected Gestations.
abstract: Human amniotic fluid (AF)-derived mesenchymal stem cells (MSCs) sharing embryonic and adult stem cells characteristics are interesting by their multipotency and the usage for regenerative medicine. However, the usefulness of these cells for revealing the fetal diseases still needs to be assessed. Here, we have analyzed the epigenetic environment in terms of histone modifications in cultures of MSCs derived from AF of normal pregnancies and those with fetal abnormalities. The comparison of MSCs samples from AF of normal pregnancies (N) and fetus-affected (P) revealed two distinct cultures by their proliferation potential (P I and P II). Cell populations from N and P I samples had similar growth characteristics and exhibited quite similar cell surface (CD44, CD90, CD105) and stemness markers (Oct4, Nanog, Sox2, Rex1) profile that was distinct in slower growing and faster senescent P II cultures. Those differences were associated with changes in 5-Cyt DNA methylation and alterations in the expression levels of chromatin modifiers (DNMT1, HDAC1/2), activating (H4ac, H3K4me3), and repressive (H3K9me2/me3, H3K27me3) histone marks. MSCs isolated from AF with the genetic or multifactorial fetal diseases (P II samples) were enriched with repressive histone marks and H4K16ac, H3K9ac, H3K14ac modifications. This study indicates that differential epigenetic environment reflects a state of AF-MSCs dependently on their growth, phenotype, and stemness characteristics suggesting a way for better understanding of epigenetic regulatory mechanisms in AF-MSCs cultures in normal and diseased gestation conditions. J. Cell. Biochem. 118: 3744-3755, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania.
authors.forename: Jūratė
authors.initials: J
authors.lastname: Savickienė
authors.affiliation: Electronics Faculty, Department Electronic Systems, Vilnius Gediminas Technical University, Vilnius LT-03227, Lithuania.
authors.forename: Dalius
authors.initials: D
authors.lastname: Matuzevičius
authors.affiliation: Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania.
authors.forename: Sandra
authors.initials: S
authors.lastname: Baronaitė
authors.affiliation: Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania.
authors.forename: Gražina
authors.initials: G
authors.lastname: Treigytė
authors.affiliation: Faculty of Medicine, Department of Human and Medical Genetics, Vilnius University, Vilnius LT-03101, Lithuania.
authors.forename: Natalija
authors.initials: N
authors.lastname: Krasovskaja
authors.affiliation: Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania.
authors.forename: Ilona
authors.initials: I
authors.lastname: Zaikova
authors.affiliation: Electronics Faculty, Department Electronic Systems, Vilnius Gediminas Technical University, Vilnius LT-03227, Lithuania.
authors.forename: Dalius
authors.initials: D
authors.lastname: Navakauskas
authors.affiliation: Faculty of Medicine, Department of Human and Medical Genetics, Vilnius University, Vilnius LT-03101, Lithuania.
authors.forename: Algirdas
authors.initials: A
authors.lastname: Utkus
authors.affiliation: Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius LT-10257, Lithuania.
authors.forename: Rūta
authors.identifier: 0000-0002-1077-9439
authors.initials: R
authors.lastname: Navakauskienė
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*
chemical_list: D006657:Histones
keywords: AMNIOTIC FLUID; EPIGENETICS; HISTONE MODIFICATIONS; STEM CELLS