title: Inflammatory and Oxidative Stress Markers in Experimental Allergic Asthma.
abstract: Ovalbumin-induced allergic lung inflammation (ALI) is a condition believed to be mediated by cytokines, extracellular matrix remodeling, and redox imbalance. In this study, we evaluated pulmonary function together with inflammatory markers as interleukin-4 (IL-4), myeloperoxidase (MPO), eosinophil cells, and redox markers in the lungs of BALB/c mice after ovalbumin (OVA) sensitization and challenge. Our results showed an increase in bronchial hyperresponsiveness stimulated by methacholine (Mch), inflammatory cell influx, especially eosinophils together with an increase of high mobility group box 1 (HMGB1) and altered lipid peroxidation (LP) and antioxidant defenses in the OVA group compared to the control group (p ≤ 0.5). Thus, we demonstrated that OVA-induced ALI altered redox status concomitantly with impaired lung function, which was associated with HMGB1 expression and proteolytic remodeling. Taken together all results found here, we may suggest HMGB1 is an important therapeutic target for asthma, once orchestrates the redox signaling, inflammation, and remodeling that contribute to the disease development.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. retiscoski@gmail.com.|Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Av. Universitária 1105, Criciúma, SC, 88806-000, Brazil. retiscoski@gmail.com.
authors.forename: Renata Tiscoski
authors.initials: RT
authors.lastname: Nesi
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.|DeVry/Fanor, Fortaleza, CE, 60191-156, Brazil.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Kennedy-Feitosa
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Manuella
authors.initials: M
authors.lastname: Lanzetti
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Mariana Barcellos
authors.initials: MB
authors.lastname: Ávila
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Clarissa Bichara
authors.initials: CB
authors.lastname: Magalhães
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Walter Araújo
authors.initials: WA
authors.lastname: Zin
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Débora Souza
authors.initials: DS
authors.lastname: Faffe
authors.affiliation: Programa de Pós-graduação em Biologia Humana e Experimental, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Luís Cristóvão
authors.initials: LC
authors.lastname: Porto
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Samuel Santos
authors.initials: SS
authors.lastname: Valença
mesh_terms: D000818:Animals; D001249:Asthma / Q000378:metabolism* / Q000473:pathology*; D015415:Biomarkers / Q000032:analysis; D016535:Bronchial Hyperreactivity; D004804:Eosinophils; D024243:HMGB1 Protein / Q000378:metabolism*; D007249:Inflammation / Q000175:diagnosis / Q000276:immunology; D015227:Lipid Peroxidation; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D018384:Oxidative Stress / Q000276:immunology; D059748:Proteolysis
chemical_list: D015415:Biomarkers; D024243:HMGB1 Protein; D010047:Ovalbumin
keywords: HMGB1; asthma; inflammation; oxidative stress; pulmonary mechanics