title: Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp.
abstract: Urinary tract infections (UTIs), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. Probiotic bacteria, especially of the genus Lactobacillus, are considered a promising preventive and/or treatment therapy against UTIs. In order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different Lactobacillus strains (Lactobacillus salivarius UCM572, L. plantarum CLC17 and L. acidophilus 01) in the adherence of reference and clinical uropathogenic strains (Escherichia coli ATCC® 53503, E. coli 10791, Enterococcus faecalis 04-1, En. faecalis 08-1 and Staphylococcus epidermidis 08-3) to T24 epithelial bladder cells. In general, the Lactobacillus strains with previous in vivo evidence of beneficial effects against UTIs (L. salivarius UCM572 and L. acidophilus 01) significantly inhibited the adherence of the five uropathogens to T24 cells, displaying percentages of inhibition ranging between 22.2% and 43.9%, and between 16.5% and 53.7%, respectively. On the other hand, L. plantarum CLC17, a strain with no expected effects on UTIs, showed almost negligible anti-adherence effects.Therefore, these in vitro results suggest that inhibition of the adherence of uropathogens to epithelial bladder cells may be one of the mechanisms involved in the potential beneficial effects of probiotics against UTIs in vivo.
authors.affiliation: Department of Food Biotechnology and Microbiology, Institute of Food Science Research, CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Dolores González
authors.initials: DG
authors.lastname: de Llano
authors.affiliation: Department of Food Biotechnology and Microbiology, Institute of Food Science Research, CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Amalia
authors.initials: A
authors.lastname: Arroyo
authors.affiliation: Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Nivia
authors.initials: N
authors.lastname: Cárdenas
authors.affiliation: Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Rodríguez
authors.affiliation: Department of Food Biotechnology and Microbiology, Institute of Food Science Research, CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Moreno-Arribas
authors.affiliation: Department of Food Biotechnology and Microbiology, Institute of Food Science Research, CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Bartolomé
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion; D002460:Cell Line; D013293:Enterococcus faecalis / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D019936:Probiotics; D013212:Staphylococcus epidermidis / Q000502:physiology*
keywords: UTI; bacteria adherence; bladder cells; probiotics; Lactobacillus; uropathogens