title: Description of Hyphopichia buzzinii f.a., sp. nov. and Hyphopichia homilentoma comb. nov., the teleomorph of Candida homilentoma.
abstract: During studies of the yeast diversity associated with rotting wood in Brazil and fruits, plants and insects in French Guiana, three strains of a new species were isolated. Analysis of the sequences of the internal transcribed spacer (ITS)-5.8S and D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the genus Hyphopichia and its closest relative is Candida homilentoma. These species differ by 44 nucleotide substitutions in D1/D2 sequences. A new species Hyphopichia buzzinii f. a., sp. nov., is proposed to accommodate these isolates. The type strain of Hyphopichia buzzinii sp. nov. is CLIB 1739T(=CBS 14300T= UFMG-CM-Y6121T; MycoBank number is MB 815609). In addition, we isolated 11 strains of C. homilentoma from rotting wood, leaf surfaces, and water bodies in Brazil, and these strains when crossed among one another and with the type strain (CBS 6312T) of this species, produced hat-shaped ascospores typical of the genus Hyphopichia. We describe the teleomorph of C. homilentoma as a new combination, Hyphopichia homilentoma comb. nov. (type strain CBS 6312T; MycoBank number is MB 820009). We also propose to transfer the other six Candida species of the Hyphopichia clade to this genus as new combinations. Hyphopichia homilentoma produced ethanol and xylitol from D-xylose whereas H. buzzinii was only able to convert this pentose to xylitol.
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Lucas R
authors.initials: LR
authors.lastname: Ribeiro
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Ana R O
authors.initials: ARO
authors.lastname: Santos
authors.affiliation: Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
authors.forename: Marizeth
authors.initials: M
authors.lastname: Groenewald
authors.affiliation: Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
authors.forename: Maudy Th H
authors.initials: MTH
authors.lastname: Smith
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Carla A
authors.initials: CA
authors.lastname: Lara
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Aristóteles
authors.initials: A
authors.lastname: Góes-Neto
authors.affiliation: Micalis Institute, INRA, AgroParisTech, CIRM-Levures, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Noémie
authors.initials: N
authors.lastname: Jacques
authors.affiliation: Micalis Institute, INRA, AgroParisTech, CIRM-Levures, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Grondin
authors.affiliation: Micalis Institute, INRA, AgroParisTech, CIRM-Levures, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Casaregola
authors.affiliation: Department of Biology, University of Western Ontario, N6A 5B7, London, ON, Canada.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
authors.affiliation: Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. carlrosa@icb.ufmg.br.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
mesh_terms: D000818:Animals; D001938:Brazil; D002175:Candida / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D016533:Mycological Typing Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Hyphopichia buzzinii; Hyphopichia homilentoma; New combinations; New yeast species; Xylitol; Xylose fermentation