title: miR-101 suppresses HBV replication and expression by targeting FOXO1 in hepatoma carcinoma cell lines.
abstract: microRNAs (miRNAs) have been identified to participate in the progression of cancers and in the infection of viruses. miR-101 expression has been found to be suppressed by HBV, however, the regulatory relationship between miR-101 and HBV replication remains elusive. In this report, miR-101 was significantly downregulated in HepG2.2.15 cells with HBV expression. miR-101 overexpression dramatically suppressed HBV replication and expression. Oppositely, overexpression of FOXO1 significantly promoted HBV replication and expression. Moreover, luciferase reporter analysis, qRT-PCR analysis and western blot assay confirmed that FOXO1 was a functional target of miR-101. Furthermore, restored FOXO1 expression abolished the inhibitory effect of miR-101 overexpression on HBV replication and expression in HepG2.2.15 cells. Our data suggested that miR-101 suppressed HBV replication and expression partially by targeting FOXO1, providing new insights into the molecular mechanisms of miR-101 in HBV-host interactions and a promising therapeutic target for HBV replication.
authors.affiliation: Department of Infectious Disease, Huaihe Hospital of Henan University, Kaifeng 475000, China. Electronic address: yanjingwang11@163.com.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Infectious Disease, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular; D000071161:Forkhead Box Protein O1 / Q000378:metabolism*; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000821:virology; D035683:MicroRNAs / Q000378:metabolism*; D014779:Virus Replication
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; C534072:MIRN101 microRNA, human; D035683:MicroRNAs
keywords: FOXO1; HBV replication; HBeAg; HBsAg; miR-101