title: A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, anddifsequences Is Effective in Construction of Recombinant Mycobacteria.
abstract: The genetic manipulation ofMycobacterium tuberculosisgenome is limited by the availability of selection markers. Spontaneous resistance mutation rate ofM. tuberculosisto the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available markers, we have created a cassette containing thiostrepton resistance gene (tsr) for selection inM. tuberculosisandM. bovisBCG, and gentamicin resistance gene (aacC1) forEscherichia coliandM. smegmatismc2155, flanked withdifsequences recognized by the Xer system of mycobacteria. This cassette adds to the limited available selection markers for mycobacteria.
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
authors.forename: Julius
authors.initials: J
authors.lastname: Mugweru
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Makafe
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Bangxing
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
authors.forename: Moses
authors.initials: M
authors.lastname: Njire
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
authors.forename: Chiranjibi
authors.initials: C
authors.lastname: Chhotaray
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital Guangzhou, China.
authors.forename: Yaoju
authors.initials: Y
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital Guangzhou, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital Guangzhou, China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital Guangzhou, China.
authors.forename: Shouyong
authors.initials: S
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences Wuhan, China.
authors.forename: Jiaoyu
authors.initials: J
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhang
keywords: gentamicin; mycobacteria; selection marker; thiostrepton