title: Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen.
abstract: Surface waters in urban areas are contaminated by ibuprofen (IBP), a popular and extensively used anti-inflammatory drug. In this study, we investigated the transcriptomic response in Arabidopsis thaliana (L.) Heynh. roots with the aim of revealing genes that are potentially involved in IBP detoxification and elucidating the effect of IBP on plants. IBP upregulated 63 and downregulated 38 transcripts (p-value < 0.1, fold change ≥2) after 2-day exposure to a 5-µM (1.03 mg/L) concentration of IBP under hydroponic conditions. Although the IBP concentration used in the experiment was highly relative to the concentrations found in rivers and wastewater, the number of genes with transcriptional changes was relatively low. The upregulation of cytochrome P450s, glutathione S-transferases, and UDP-glycosyltransferases indicates the occurrence of IBP oxidation in the first phase, followed by conjugation with glutathione and sugar in the second detoxification phase. ABC transporters could be involved in the transport of IBP and its metabolites. The identification of genes potentially involved in IBP detoxification could be useful in an IBP phytoremediation approach.
authors.affiliation: a Laboratory of Plant Biotechnologies , Institute of Experimental Botany AS CR, v.v.i. , Prague , Lysolaje , Czech Republic.
authors.forename: Premysl
authors.initials: P
authors.lastname: Landa
authors.affiliation: b Laboratory of Hormonal Regulations in Plants , Institute of Experimental Botany AS CR, v.v.i. , Prague , Lysolaje , Czech Republic.|c Department of Experimental Plant Biology, Faculty of Science , Charles University in Prague , Prague , Czech Republic.
authors.forename: Sylva
authors.initials: S
authors.lastname: Prerostova
authors.affiliation: a Laboratory of Plant Biotechnologies , Institute of Experimental Botany AS CR, v.v.i. , Prague , Lysolaje , Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Langhansova
authors.affiliation: a Laboratory of Plant Biotechnologies , Institute of Experimental Botany AS CR, v.v.i. , Prague , Lysolaje , Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Marsik
authors.affiliation: a Laboratory of Plant Biotechnologies , Institute of Experimental Botany AS CR, v.v.i. , Prague , Lysolaje , Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Vanek
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D007052:Ibuprofen / Q000494:pharmacology / Q000633:toxicity*; D018517:Plant Roots; D014158:Transcription, Genetic / Q000187:drug effects*; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D007052:Ibuprofen; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: detoxification; gene expression; metabolism of xenobiotics; microarrays; phytoremediation