title: Development of drug-loaded chitosan hollow nanoparticles for delivery of paclitaxel to human lung cancer A549 cells.
abstract: In this study, biodegradable chitosan hollow nanospheres (CHN) were fabricated using polystyrene nanospheres (PS) as templates. CHN were applied to increase the solubility of poorly water-soluble drugs. The lung cancer drug paclitaxel (PTX), which is used as a model drug, was loaded into CHN by the adsorption equilibrium method. The drug-loaded sample (PTX-CHN) offered sustained PTX release and good bioavailability. The state characterization of PTX by differential scanning calorimetry (DSC), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) showed that the PTX absorbed into CHN existed in an amorphous state. An in vitro toxicity experiment indicated that CHN were nontoxic as carriers of poorly water-soluble drugs. The PTX-CHN produced a marked inhibition of lung cancer A549 cells proliferation and encouraged apoptosis. A cell uptake experiment indicated that PTX-CHN was successfully taken up by lung cancer A549 cells. Furthermore, a degradation experiment revealed that CHN were readily biodegradable. These findings state clearly that CHN can be regarded as promising biomaterials for lung cancer treatment.
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Lv
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Andi
authors.initials: A
authors.lastname: Bai
authors.affiliation: a Pharmacy School, Jinzhou Medical University , Jinzhou , PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000072283:A549 Cells / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D001682:Biological Availability; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D008168:Lung / Q000737:chemistry / Q000378:metabolism*; D008175:Lung Neoplasms / Q000737:chemistry / Q000188:drug therapy*; D053758:Nanoparticles / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry*; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D048271:Chitosan; D017239:Paclitaxel
keywords: Chitosan hollow nanospheres; biodegradable; lung cancer A549 cells; paclitaxel; sustained release