title: A novel perivascular cell population in the zebrafish brain.
abstract: The blood-brain barrier is essential for the proper homeostasis and function of the CNS, but its mechanism of function is poorly understood. Perivascular cells surrounding brain blood vessels are thought to be important for blood-brain barrier establishment, but their roles are not well defined. Here, we describe a novel perivascular cell population closely associated with blood vessels on the zebrafish brain. Based on similarities in their morphology, location, and scavenger behavior, these cells appear to be the zebrafish equivalent of cells variably characterized as Fluorescent Granular Perithelial cells (FGPs), perivascular macrophages, or 'Mato Cells' in mammals. Despite their macrophage-like morphology and perivascular location, zebrafish FGPs appear molecularly most similar to lymphatic endothelium, and our imaging studies suggest that these cells emerge by differentiation from endothelium of the optic choroidal vascular plexus. Our findings provide the first report of a perivascular cell population in the brain derived from vascular endothelium.
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Marina
authors.identifier: 0000-0002-0262-1754
authors.initials: M
authors.lastname: Venero Galanternik
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Castranova
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Aniket V
authors.initials: AV
authors.lastname: Gore
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Nathan H
authors.initials: NH
authors.lastname: Blewett
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Hyun Min
authors.initials: HM
authors.lastname: Jung
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Amber N
authors.initials: AN
authors.lastname: Stratman
authors.affiliation: Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, United States.
authors.forename: Martha R
authors.initials: MR
authors.lastname: Kirby
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: James
authors.initials: J
authors.lastname: Iben
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Mayumi F
authors.initials: MF
authors.lastname: Miller
authors.affiliation: Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan.|Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Japan.
authors.forename: Koichi
authors.identifier: 0000-0001-9993-1435
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Maraia
authors.affiliation: Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
authors.forename: Brant M
authors.identifier: 0000-0003-4136-4962
authors.initials: BM
authors.lastname: Weinstein
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D001812:Blood-Brain Barrier / Q000166:cytology*; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation; D042783:Endothelial Cells / Q000166:cytology*; D015027:Zebrafish
keywords: Fluorescent Granular Periendothelial cells; Mato Cells; Perivascular macrophages; developmental biology; lymphatics; mouse; neuroscience; stem cells; vessels; zebrafish; zebrafish brain