title: P2Y6-deficiency increases micturition frequency and attenuates sustained contractility of the urinary bladder in mice.
abstract: The role of the P2Y6receptor in bladder function has recently attracted a great deal of attention in lower urinary tract research. We conducted this study to determine contributions of the P2Y6receptor in lower urinary tract function of normal phenotypes by comparing P2Y6-deficient mice and wild-type mice. In in vivo experiments, P2Y6-deficient mice had more frequent micturition with smaller bladder capacity compared to wild-type mice; however, there was no difference between these groups in bladder-filling pressure/volume relationships during cystometry under decerebrate, unanaesthetized conditions. Analysis of in vivo bladder contraction revealed significant difference between the 2 groups, with P2Y6-deficient mice presenting markedly shorter bladder contraction duration but no difference in peak contraction pressure. However, analysis of in vitro experiments showed no P2Y6involvements in contraction and relaxation of bladder muscle strips and in ATP release by mechanical stimulation of primary-cultured urothelial cells. These results suggest that the P2Y6receptor in the central nervous system, dorsal root ganglion, or both is involved in inhibition of bladder afferent signalling or sensitivity in the pontine micturition centre and that the receptor in the detrusor may be implicated in facilitation to sustain bladder contraction force.
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kira
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan. PXN15164@nifty.ne.jp.
authors.forename: Mitsuharu
authors.identifier: http://orcid.org/0000-0001-8695-884X
authors.initials: M
authors.lastname: Yoshiyama
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsuchiya
authors.affiliation: Department of Neuropharmacology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.|Japan Science and Technology Agency, CREST, Tokyo, 102-0076, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Shigetomi
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakagomi
authors.affiliation: Department of Neuropharmacology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.|Japan Science and Technology Agency, CREST, Tokyo, 102-0076, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Shibata
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Mochizuki
authors.affiliation: Department of Urology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takeda
authors.affiliation: Department of Neuropharmacology, University of Yamanashi Graduate School of Medical Science, Yamanashi, 409-3898, Japan.|Japan Science and Technology Agency, CREST, Tokyo, 102-0076, Japan.
authors.forename: Schuichi
authors.initials: S
authors.lastname: Koizumi
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D015870:Gene Expression; D051379:Mice; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000187:drug effects / Q000235:genetics*; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000172:deficiency* / Q000235:genetics; D012018:Reflex; D001743:Urinary Bladder / Q000378:metabolism / Q000503:physiopathology*; D014554:Urination
chemical_list: D000318:Adrenergic beta-Agonists; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; C099845:purinoceptor P2Y6; D000255:Adenosine Triphosphate