title: High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okano
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Baba
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yamasaki
authors.affiliation: Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, Hyogo, 669-1337, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Hidese
authors.affiliation: Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, Hyogo, 669-1337, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Fujiwara
authors.affiliation: Department of Developmental Medicine, Osaka Medical Center and Research Institute for Maternal and Child Health, Osaka, 594-1101, Japan.
authors.forename: Itaru
authors.initials: I
authors.lastname: Yanagihara
authors.affiliation: Kainos Laboratories, Inc., 38-18, Hongo 2-chome, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ujiiye
authors.affiliation: Kainos Laboratories, Inc., 38-18, Hongo 2-chome, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
authors.forename: Teisuke
authors.initials: T
authors.lastname: Takita
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan. Electronic address: yasukawa@kais.kyoto-u.ac.jp.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yasukawa
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008979:Moloney murine leukemia virus / Q000201:enzymology / Q000235:genetics; D015202:Protein Engineering; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D019712:Thermococcus / Q000201:enzymology / Q000235:genetics
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases; D020365:RNA Helicases; D008274:Magnesium
keywords: DNA polymerase; Helicase; Magnesium ion; Manganese ion; One-step RT-PCR; Reverse transcriptase