title: Ribosomal background of the Bacillus cereus group thermotypes.
abstract: In this study we reconstructed the architecture of Bacillus cereus sensu lato population based on ribosomal proteins, and identified a link between the ribosomal proteins' variants and thermal groups (thermotypes) of the bacilli. The in silico phyloproteomic analysis of 55 ribosomal proteins (34 large and 21 small subunit r-proteins) of 421 strains, representing 14 well-established or plausible B. cereus sensu lato species, revealed several ribosomal clusters (r-clusters), which in general were well correlated with the strains' affiliation to phylogenetic/thermal groups I-VII. However, a conformity and possibly a thermal characteristic of certain phylogenetic groups, e.g. the group IV, were not supported by a distribution of the corresponding r-clusters, and consequently neither by the analysis of cold-shock proteins (CSPs) nor by a content of heat shock proteins (HSPs). Furthermore, a preference for isoleucine and serine over valine and alanine in r-proteins along with a lack of HSP16.4 were recognized in non-mesophilic thermotypes. In conclusion, we suggest that the observed divergence in ribosomal proteins may be connected with an adaptation of B. cereus sensu lato members to various thermal niches.
authors.affiliation: Department of Microbiology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Fiedoruk
authors.affiliation: Department of Microbiology, Institute of Biology, University of Bialystok, Bialystok, Poland.
authors.forename: Justyna M
authors.initials: JM
authors.lastname: Drewnowska
authors.affiliation: Department of Microbiology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Tamara
authors.initials: T
authors.lastname: Daniluk
authors.affiliation: Department of Microbiology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Leszczynska
authors.affiliation: Department of Microbiology, Institute of Biology, University of Bialystok, Bialystok, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Iwaniuk
authors.affiliation: Department of Microbiology, Institute of Biology, University of Bialystok, Bialystok, Poland.|Laboratory of Applied Microbiology, University of Bialystok, Bialystok, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Swiecicka
mesh_terms: D000222:Adaptation, Physiological; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003198:Computer Simulation; D014644:Genetic Variation; D010802:Phylogeny; D040901:Proteomics / Q000379:methods*; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins