title: Immunological response to Mycobacterium tuberculosis infection in blood from type 2 diabetes patients.
abstract: The convergence of tuberculosis and diabetes represents a co-epidemic that threatens progress against tuberculosis. We have investigated type 2 diabetes as a risk factor for tuberculosis susceptibility, and have used as experimental model whole blood infected in vitro with Mycobacterium tuberculosis. Blood samples from diabetic patients were found to have a higher absolute neutrophil count that non-diabetic controls, but their immune functionality seemed impaired because they displayed a lower capacity to phagocytose M. tuberculosis, a finding that had been previously reported only for monocytes. In contrast, an increased production of TNFα was detected in infected blood from diabetic patients. Despite the altered phagocytic capacity showed by cells from these patients, the antimicrobial activity measured in both whole blood and monocyte derived macrophages was similar to that of controls. This unexpected result prompts further improvements in the whole blood model to analyze the immune response of diabetes patients to tuberculosis.
authors.affiliation: Servicio de Medicina Interna, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Raposo-García
authors.affiliation: Servicio de Medicina Interna, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Guerra-Laso
authors.affiliation: Servicio de Neumología, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: García-García
authors.affiliation: Servicio de Neumología, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Juan-García
authors.affiliation: Unidad de Investigación, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: López-Fidalgo
authors.affiliation: Servicio de Anatomía Patológica, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain; Institute of Biomedicine (IBIOMED), University of León, León, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Diez-Tascón
authors.affiliation: Servicio de Microbiología Clínica, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Nebreda-Mayoral
authors.affiliation: Servicio de Microbiología, Hospital Comarcal El Bierzo, León, Spain.
authors.forename: Ramiro
authors.initials: R
authors.lastname: López-Medrano
authors.affiliation: Unidad de Investigación, Complejo Asistencial Universitario de León, Altos de Nava s/n, 24008 León, Spain; Institute of Biomedicine (IBIOMED), University of León, León, Spain; Fundación Instituto de Estudios de Ciencias de la Salud de Castilla y León, Soria, Spain. Electronic address: omrivero@iecscyl.com.
authors.forename: Octavio Miguel
authors.initials: OM
authors.lastname: Rivero-Lezcano
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001773:Blood Cells / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D012306:Risk; D014376:Tuberculosis / Q000150:complications / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha
keywords: Antimycobacterial activity; Macrophages; Mycobacterium tuberculosis; Neutrophils; Tumor necrosis factor alpha; Type 2 diabetes