title: tRF-Leu-CAG promotes cell proliferation and cell cycle in non-small cell lung cancer.
abstract: tRNA-derived RNA fragments (tRFs), non-coding single-stranded RNAs with 14-35 nt in length, were found to play important roles in gene regulation, even in carcinogenesis. In this study, we investigated the expression of tRF-Leu-CAG in human non-small cell lung cancer (NSCLC) and its function in the cell proliferation and cell cycle of NSCLC. The expression level of tRF-Leu-CAG was detected in NSCLC tissues, cell lines, and sera. tRF-Leu-CAG RNA levels were higher in NSCLC tumor tissues than in normal tissues, and also upregulated in NSCLC cell lines. A significant relationship was observed between stage progression and tRF-Leu-CAG in NSCLC sera. We found that in H1299 cells, inhibition of tRF-Leu-CAG suppressed cell proliferation and impeded cell cycle. AURKA was also repressed with the knockdown of tRF-Leu-CAG. Thus, our study revealed that tRF-Leu-CAG may be involved in regulating AURKA and could be a new diagnostic marker and potential therapeutic target in NSCLC.
authors.affiliation: Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.|Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Yang
authors.identifier: http://orcid.org/0000-0002-3239-4265
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Central Laboratory, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Qiangling
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.|Experiment Center for Life Sciences, Shanghai University, Shanghai, China.
authors.forename: Renqi
authors.initials: R
authors.lastname: Wu
authors.affiliation: Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding
keywords: NSCLC; cell cycle; diagnostic biomarker; proliferation; tRF-Leu-CAG