title: Layer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering.
abstract: The conventional tissue engineering is based on seeding of macroporous scaffold on its surface ("top-down" approach). The main limitation is poor cell viability in the middle of the scaffold due to poor diffusion of oxygen and nutrients and insufficient vascularization. Layer-by-Layer (LBL) bioassembly is based on "bottom-up" approach, which considers assembly of small cellularized blocks. The aim of this work was to evaluate proliferation and differentiation of human bone marrow stromal cells (HBMSCs) and endothelial progenitor cells (EPCs) in two and three dimensions (2D, 3D) using a LBL assembly of polylactic acid (PLA) scaffolds fabricated by 3D printing. 2D experiments have shown maintain of cell viability on PLA, especially when a co-cuture system was used, as well as adequate morphology of seeded cells. Early osteoblastic and endothelial differentiations were observed and cell proliferation was increased after 7 days of culture. In 3D, cell migration was observed between layers of LBL constructs, as well as an osteoblastic differentiation. These results indicate that LBL assembly of PLA layers could be suitable for BTE, in order to promote homogenous cell distribution inside the scaffold and gene expression specific to the cells implanted in the case of co-culture system.
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France.|Fakultet Tehnickih Nauka, Univerzitet u Novom Sadu, Trg Dositeja Obradovica 3, Novi Sad, 21000, Serbia.
authors.forename: Vera
authors.identifier: http://orcid.org/0000-0003-1634-285X
authors.initials: V
authors.lastname: Guduric
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Metz
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France.
authors.forename: Robin
authors.initials: R
authors.lastname: Siadous
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France.
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.affiliation: Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Levato
authors.affiliation: Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engel
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Fricain
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Devillard
authors.affiliation: Fakultet Tehnickih Nauka, Univerzitet u Novom Sadu, Trg Dositeja Obradovica 3, Novi Sad, 21000, Serbia.
authors.forename: Ognjan
authors.initials: O
authors.lastname: Luzanin
authors.affiliation: Biotis, Inserm U1026, Université Bordeaux Segalen, 146 rue Léo-Saignat, Case 45, Bordeaux Cedex, 33076, France. sylvain.catros@u-bordeaux.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Catros
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D001842:Bone and Bones / Q000473:pathology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D010100:Oxygen / Q000737:chemistry; D010641:Phenotype; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D066330:Printing, Three-Dimensional; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C033616:poly(lactide); D010100:Oxygen