title: The Research on the Relationship of RAGE, LRP-1, and Aβ Accumulation in the Hippocampus, Prefrontal Lobe, and Amygdala of STZ-Induced Diabetic Rats.
abstract: Diabetes mellitus (DM) has been regarded as an important risk factor for Alzheimer's disease (AD), and diabetic patients and animals have shown cognitive dysfunction. More research has shown that the amyloid-β (Aβ), which is a hallmark of AD, was found deposited in the hippocampus of diabetic rats. This Aβ accumulation is regulated by the receptor for advanced glycation end products (RAGE) and low-density lipoprotein receptor-related protein (LRP-1). However, the expression of RAGE and LRP-1 in diabetic rats is not very clear. In the present study, we used streptozotocin (STZ)-induced diabetic rats to investigate whether the expression of RAGE and LRP-1 is related to Aβ1-42deposition at the hippocampus, prefrontal lobe, and amygdala in DM. We found that diabetic rats had longer escape latency and less frequency of entrance into the target zone than that of the control group (P < 0.05) in the Morris water maze (MWM) test. The Aβ1-42expression in the hippocampus and prefrontal lobe significantly increased in the DM group compared to the control group (P < 0.05). RAGE increased (P < 0.05), while LRP-1 decreased (P < 0.05) in the hippocampus tissue and prefrontal lobe tissue of DM rats. The Aβ1-42deposition was correlated with RAGE positively (P < 0.05), but with LRP-1 negatively (P < 0.05). Further, the expression levels of Aβ1-42, RAGE, and LRP-1 were not changed in the amygdala between the diabetic rats and the control group. These findings indicated that upregulating RAGE and/or downregulating LRP-1 at the hippocampus and the prefrontal lobe contributed to the Aβ1-42accumulation and then further promoted the cognitive impairment of diabetic rats.
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Lou-Yan
authors.initials: LY
authors.lastname: Ma
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yu-Lang
authors.initials: YL
authors.lastname: Fei
authors.affiliation: Department of science and education, Xi'an Ninth Hospital, Xi'an, China.
authors.forename: Xiao-Ye
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Neurology, First Hospital of Xi'an, Xi'an, China.
authors.forename: Song-Di
authors.initials: SD
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, Xi'an Ninth Hospital, Xi'an, China.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Du
authors.affiliation: Department of Psychology and Psychiatry, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhu
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Li
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Zhai
authors.affiliation: Department of Internal Medicine (1), the Fifth People's Hospital of Zhuhai, Guangdong, China.
authors.forename: Lu-Peng
authors.initials: LP
authors.lastname: Ji
authors.affiliation: Department of Neurology, Xi'an Ninth Hospital, Xi'an, China.
authors.forename: Ran-Ran
authors.initials: RR
authors.lastname: Ma
authors.affiliation: Department of Endocrinology, Xi'an Ninth Hospital, Xi'an, China.
authors.forename: Song-Fang
authors.initials: SF
authors.lastname: Liu
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Ma
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. quqiumin@medmail.com.cn.
authors.forename: Qiu-Min
authors.initials: QM
authors.lastname: Qu
authors.affiliation: The Second Department of Geriatrics, Xi'an Ninth Hospital, No. 151, the Second Ring South Road, Xi'an, 710054, China. dryalilv@163.com.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Lv
mesh_terms: D000679:Amygdala / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D003071:Cognition; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018782:Maze Learning; D017397:Prefrontal Cortex / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D000067759:Receptor for Advanced Glycation End Products
keywords: Amyloid; Cognition; Diabetes; LRP-1; Rage