title: A cancer cell-specific fluorescent probe for imaging Cu2+in living cancer cells.
abstract: Monitoring copper level in cancer cells is important for the further understanding of its roles in the cell proliferation, and also could afford novel copper-based strategy for the cancer therapy. Herein, we have developed a novel cancer cell-specific fluorescent probe for the detecting Cu2+in living cancer cells. The probe employed biotin as the cancer cell-specific group. Before the treatment of Cu2+, the probe showed nearly no fluorescence. However, the probe can display strong fluorescence at 581nm in response to Cu2+. The probe exhibited excellent sensitivity and high selectivity for Cu2+over the other relative species. Under the guidance of biotin group, could be successfully used for detecting Cu2+in living cancer cells. We expect that this design strategy could be further applied for detection of the other important biomolecules in living cancer cells.
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Dong
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China.
authors.forename: Xiuqi
authors.initials: X
authors.lastname: Kong
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China.
authors.forename: Xuezhen
authors.initials: X
authors.lastname: Song
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China. Electronic address: weiyinglin2013@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D003300:Copper / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D041681:NIH 3T3 Cells; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D003300:Copper
keywords: Cancer cell; Copper; Fluorescence imaging; Fluorescent probe