title: Dissolving Microneedles as Skin Allergy Test Device.
abstract: The possibility of using dissolving microneedles (DMs) as a skin allergy test device was studied in rats. Poly-L-arginine was used as a model allergen. Dextran was used to prepare three kinds of DM array chips containing different doses of poly-L-arginine: 17.1±0.5 µg (low-dose DM), 42.2±0.8 µg (medium-dose DM), and 87.4±1.1 µg (high-dose DM); each 1.0 cm2chip contained 300 DMs. The mean lengths of the low-, medium-, and high-dose DM were 489±3, 485±3, and 492±1 µm and mean diameters of the base were 301±2, 299±1, and 299±2 µm, respectively. Furthermore, for the low-, medium-, and high-dose DM, the administered doses of poly-L-arginine were estimated to be 9.3±1.9, 31.1±1.3, and 61.9±4.7 µg and the scratching behavior per 30 min was 9.8±3.4, 60.4±8.3, and 95.7±10.6 times, respectively. These results demonstrate the dose dependence of the immunoreactivity of the poly-L-arginine DMs, suggesting that DMs can be used an alternative skin allergy device.
authors.affiliation: Department of Pharmacokinetics, Kyoto Pharmaceutical University.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Ito
authors.forename: Kengo
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Osakama
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Yoshioka
authors.forename: Shinji
authors.initials: S
authors.lastname: Kobuchi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaeda
authors.forename: Kanji
authors.initials: K
authors.lastname: Takada
mesh_terms: D000279:Administration, Cutaneous; D000485:Allergens / Q000008:administration & dosage* / Q000009:adverse effects; D000818:Animals; D004305:Dose-Response Relationship, Drug; D006967:Hypersensitivity / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008845:Microinjections / Q000379:methods*; D009339:Needles; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption / Q000187:drug effects* / Q000502:physiology; D012882:Skin Tests / Q000379:methods
chemical_list: D000485:Allergens