title: [Allelic diversity of KIR2DL4 gene and identification of five novel alleles among southern Han Chinese population].
abstract: OBJECTIVE
  To study the polymorphisms of the KIR2DL4 gene in a southern Han Chinese population.
  
  
  METHODS
  Genomic DNA isolated from 306 unrelated individuals was amplified by using KIR2DL4-specific PCR primers. The PCR products were genotyped for the entire coding sequence by sequencing-based typing (SBT). Assignment of allelic profile was accomplished with an Assign 3.5 software. For samples with inconclusive SBT results, the RT-PCR products covering the entire coding sequence of the KIR2DL4 gene were subjected to cloning and haplotype sequencing.
  
  
  RESULTS
  Among the 306 individuals, 11 alleles were detected, of which 5 novel alleles were officially named by the KIR subcommittee of the World Health Organization Nomenclature Committee for factors of HLA system. The observed frequencies for the 11 alleles were KIR2DL4 *00102 (75.5%), *00103 (8.2%), *00501 (34.0%), *00503 (0.7%), *00504 (0.7%), *00602 (14.4%), *00801 (11.4%), *011 (22.2%), *032 (0.3%), *033 (0.3%) and *034 (0.3%). The ratio of 10A type alleles including 2DL4*00102, *00103, *00501, *00503, *00504, *00602, *032, *033, *034 and 9A type alleles including 2DL4*00801, *011 were 97.0% and 33.0%, respectively, with a ratio of 2.9:1.
  
  
  CONCLUSIONS
  The allelic diversity of the KIR2DL4 gene in southern Han Chinese has been elucidated, which may provide valuable data for research on transplantation, reproductive immunity, KIR-associated disease and evolution.
authors.affiliation: Southern Medical University, Guangzhou, Guangdong 510515, China; Shenzhen Blood Center, Shenzhen, Guangdong 518035, China. zhihui_deng@aliyun.com.
authors.forename: Guobin
authors.initials: G
authors.lastname: Zhang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D054345:Receptors, KIR2DL4 / Q000235:genetics*
chemical_list: C515079:KIR2DL4 protein, human; D054345:Receptors, KIR2DL4