title: Chikusetsu (CHI) triggers mitochondria-regulated apoptosis in human prostate cancer via reactive oxygen species (ROS) production.
abstract: The prostate cancer prognosis is still not fully understood. Chikusetsu saponin Iva (CHI), isolated from Aralia taibaiensis, shows anti-cancer and anti-inflammatory properties. Here, in our study, we attempted to explore the efficiency and the possible molecular mechanism by which CHI may suppress prostate cancer. CHI was found to inhibit prostate cancer cell proliferation and induce cell death without cytotoxicity in prostate normal cells. CHI resulted in intracellular reactive oxygen species (ROS) production, and induced apoptosis regulated by mitochondria in vitro studies. CHI-caused apoptosis was shown in both caspase-dependent and -independent manner, which released cyto-c, enhancing caspases expression and promoting apoptosis-inducing factors (AIF) as well as endonuclease G (Endo G) nuclear transfer, respectively. Moreover, in vivo study showed that prostate tumor was inhibited by CHI administration through apoptosis induction. Thus, the results illustrated that CHI might be an effective therapeutic strategy for prostate cancer treatment in future.
authors.affiliation: Department of Urology, Linyi People's Hospital, Linyi, 27 East Jiefang Rd, Lanshan, Linyi, Shandong, PR China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Zhu
authors.affiliation: Department of Dermatology, Linyi People's Hospital, Linyi, 27 East Jiefang Rd, Lanshan, Linyi, Shandong, PR China.
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Tian
authors.affiliation: Department of Dermatology, Linyi People's Hospital, Linyi, 27 East Jiefang Rd, Lanshan, Linyi, Shandong, PR China. Electronic address: liuliqianlinyi@foxmail.com.
authors.forename: Li-Qian
authors.initials: LQ
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004706:Endodeoxyribonucleases / Q000378:metabolism; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D051033:Apoptosis Inducing Factor; D017382:Reactive Oxygen Species; D012503:Saponins; C098580:chikusetsu saponin IVa; D009828:Oleanolic Acid; D004706:Endodeoxyribonucleases; C052391:endonuclease G; D020169:Caspases
keywords: Apoptosis; Chikusetsu saponin Iva; Prostate cancer; ROS