title: Intercalation of Alkynylplatinum(II) Terpyridine Complexes into a Helical Poly(phenylene ethynylene) Sulfonate: Application to Protein Sensing.
abstract: The interactions of two anionic poly(phenylene ethynylene) sulfonate-conjugated polyelectrolytes (mPPESO3-and pPPESO3-) with two alkynylplatinum(II) terpyridine complexes (Pt2+and Pt3+) were studied. The Pt(II) complexes interact with helical mPPESO3-by intercalation within the polymer helix to form a "guest-host" ensemble. Titration of Pt(II) complexes into an aqueous solution of mPPESO3-gives rise to efficient quenching of the polymer's fluorescence; meanwhile, triplet metal-metal-to-ligand charge transfer (3MMLCT) state emission from the intercalated Pt(II) complexes appears when the ensembles are excited into the polymer's absorption band. The3MMLCT state emission implies that the Pt(II) complexes aggregate or dimerize on the mPPESO3-scaffold. The responses of the mPPESO3-and Pt(II) complex ensembles to various proteins were examined by monitoring the mPPESO3-fluorescence change. Negatively charged proteins recover the mPPESO3-fluorescence more than the positively charged proteins under physiological pH, indicating that electrostatics play an important role in the protein-ensemble interaction.
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Zeman
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Junlin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.|Department of Chemistry, University of Texas at San Antonio , One UTSA Way, San Antonio, Texas 78249, United States.
authors.forename: Kirk S
authors.identifier: http://orcid.org/0000-0003-3342-4080
authors.initials: KS
authors.lastname: Schanze
mesh_terms: D000476:Alkanesulfonates; D008024:Ligands; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D011506:Proteins
chemical_list: D000476:Alkanesulfonates; D008024:Ligands; D011108:Polymers; D011506:Proteins; C473966:poly(phenylene ethynylene)
keywords: conjugated polyelectrolyte; fluorescence quenching; intercalation; platinum terpyridyl complex; protein sensing