title: Autologous Bone Marrow Cell Stimulation and Allogenic Chondrocyte Implantation for the Repair of Full-Thickness Articular Cartilage Defects in a Rabbit Model.
abstract: The aim of this study was to evaluate the results of autologous bone marrow cell stimulation and allogenic chondrocyte implantation using 3-dimensional gel-type fibrin matrix in an animal model.
  
  
  
  Eighteen rabbits were divided into 2 treatment groups. One group was treated with a microfracture and covering of it with gel-type fibrin (AutoBMS; n = 9), and the other group was treated with allogenic chondrocytes mixed gel-type fibrin at the cartilage defect (AlloCI; n = 9). The control group was untreated cartilage defect at the other side knee of each object. Twelve weeks after treatment, the cartilage was evaluated using the International Cartilage Repair Society (ICRS) scoring system, immunohistochemical staining, and modified O'Driscoll grading system.
  
  
  
  The ICRS scores were similar in the AutoBMS (9.44 ± 2.44) and the AlloCI (9.33 ± 1.67) groups ( P < 0.05). Immunohistochemical staining confirmed higher expression of cartilaginous collagen for both groups. The average difference (AutoBMS, 31.89 ± 6.54; AlloCI, 32.89 ± 5.25) in the modified O'Driscoll scores appeared to be nonsignificant ( P > 0.05); however, both treatment groups showed significantly higher scores with respect to their control group (18.45 ± 1.65; 18.97 ± 1.58) ( P < 0.05).
  
  
  
  This experimental study suggests autologous bone marrow cells stimulation and implantation of allogenic chondrocytes are both useful methodologies for regenerating hyaline-like cartilage in full-thickness cartilage defects in animal model.
authors.affiliation: 1 Department of Orthopedic Surgery, Jeju National University, Jeju, Republic of Korea.
authors.forename: Sungwook
authors.initials: S
authors.lastname: Choi
authors.affiliation: 1 Department of Orthopedic Surgery, Jeju National University, Jeju, Republic of Korea.
authors.forename: Gyeong Min
authors.initials: GM
authors.lastname: Kim
authors.affiliation: 2 Department of Pathology, Jeju National University, Jeju, Republic of Korea.
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Maeng
authors.affiliation: 1 Department of Orthopedic Surgery, Jeju National University, Jeju, Republic of Korea.
authors.forename: Hyunseong
authors.initials: H
authors.lastname: Kang
authors.affiliation: 1 Department of Orthopedic Surgery, Jeju National University, Jeju, Republic of Korea.
authors.forename: Chen Tai
authors.initials: CT
authors.lastname: Teong
authors.affiliation: 3 Shiley Center for Orthopaedic Research & Education at Scripps Clinic, La Jolla, CA, USA.
authors.forename: Emily E
authors.initials: EE
authors.lastname: Lee
authors.affiliation: 1 Department of Orthopedic Surgery, Jeju National University, Jeju, Republic of Korea.
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Yoo
authors.affiliation: 3 Shiley Center for Orthopaedic Research & Education at Scripps Clinic, La Jolla, CA, USA.
authors.forename: Darryl D
authors.initials: DD
authors.lastname: Dlima
authors.affiliation: 4 Department of Orthopedic Surgery, Inha University Hospital, Incheon, Republic of Korea.
authors.forename: Myung Ku
authors.initials: MK
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002357:Cartilage Diseases / Q000628:therapy*; D002358:Cartilage, Articular / Q000166:cytology*; D019902:Chondrocytes / Q000637:transplantation*; D004195:Disease Models, Animal; D011817:Rabbits; D033581:Stem Cell Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
keywords: allogenic chondrocyte; autologous bone marrow cell stimulation; cartilage repair