title: Polymer-modified fibrous mesoporous silica nanoparticles as coating material for open-tubular capillary electrochromatography.
abstract: A novel fibrous mesoporous silica nanoparticles (fSiO2) stationary phase grafted with polymer (Poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) was developed for open tubular capillary electrochromatography (OT-CEC). The preparation procedure included synthesizing fSiO2through biphase stratification approach, removing the surfactants, silanization and in situ graft polymerization with monomers via atom transfer radical polymerization (ATRP). Subsequently, PDMAEMA-modified mesoporous silica nanoparticles (P-fSiO2)/ethanol solution was immobilized onto the inner surface of the pretreated capillary and functionalized with octadecylsilane to fabricate the open-tubular column. Separation of polycyclic aromatic hydrocarbons (PAHs) and proteins were carried out to evaluate the performance of the column in CEC. The run-to-run, day-to-day and column-to-column reproducibility in terms retention time of naphthalene was 1.9%, 2.2%, and 3.7%, respectively. The effects of solvent concentration and pH on the separation were evaluated. The method was also used for the separation of real bio-sample, egg white proteins.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Molecule Design and Interface Process, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China.
authors.forename: Qishu
authors.initials: Q
authors.lastname: Qu
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: chaoyan@sjtu.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D008689:Methacrylates; D053758:Nanoparticles / Q000737:chemistry*; D009757:Nylons; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D009757:Nylons; D011084:Polycyclic Aromatic Hydrocarbons; D011108:Polymers; D011506:Proteins; C407037:poly(2-(dimethylamino)ethyl methacrylate); D012822:Silicon Dioxide
keywords: Fibrous mesoporous silica nanoparticles; Open-tubular capillary electrochromatography; Polymer; Stationary phase