title: Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer.
abstract: Despite complete resection of the early stage of oral tongue cancer by partial glossectomy, late cervical lymph node metastasis is frequently observed. Gene amplification of ACTN4 (protein name: actinin-4) is closely associated with the metastatic potential of various cancers. This retrospective study was performed to demonstrate the potential usefulness of ACTN4 gene amplification as a prognostic biomarker in patients with stage I/II oral tongue cancer. Fifty-four patients with stage I/II oral tongue cancer were enrolled retrospectively, in accordance with the reporting recommendations for tumour marker prognostic studies (REMARK) guidelines. The copy number of ACTN4 and the protein expression of actinin-4 were evaluated by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), respectively. The overall survival time of patients with gene amplification of ACTN4 was significantly shorter than that of patients without gene amplification (P=0.0010, log-rank test). Gene amplification of ACTN4 was a significant independent risk factor for death in patients with stage I/II oral tongue cancer (hazard ratio 6.08, 95% confidence interval 1.66-22.27). Gene amplification of ACTN4 is a potential prognostic biomarker for overall survival in oral tongue cancer.
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo, Japan; Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kakuya
authors.affiliation: Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Head and Neck Oncology, National Cancer Center Hospital, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshimoto
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Watabe
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miura
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shoji
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Onidani
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shibahara
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo, Japan; Japan Agency for Medical Research and Development (AMED) CREST, Tokyo, Japan. Electronic address: khonda@ncc.go.jp.
authors.forename: K
authors.initials: K
authors.lastname: Honda
mesh_terms: D000185:Actinin / Q000235:genetics*; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D005927:Glossectomy; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies; D012307:Risk Factors; D015996:Survival Rate; D014062:Tongue Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery
chemical_list: C111453:ACTN4 protein, human; D014408:Biomarkers, Tumor; D000185:Actinin
keywords: ACTN4; early stage oral tongue cancer; gene amplification; prognostic biomarker