title: Flavonoids of Rosa roxburghii Tratt Exhibit Anti-Apoptosis Properties by Regulating PARP-1/AIF.
abstract: Radioprotection is an important approach to reduce the side-effects of radiotherapy. The radioprotective effect of the flavonoids of Rosa roxburghii Tratt (FRT) has been confirmed, and the mechanism has been identified as theBcl-2/caspase-3/PARP-1 signaling pathway. In this study, we investigated the effects of FRT on the intercellular adhesion molecule (ICAM), and vascular cell adhesion protein (VCAM) in addition to apoptosis-related proteins such as Bax/Bcl-2, p-ERK/ERK, p-p53/p53, and p-p38/p38. In the present study, we focused on the effect of FRT on PARP-1/AIF. Ionizing radiation triggered the activation of PARP-1 and AIF translocation from the mitochondrion to the nucleus. The inhibition of PARP-1/AIF signaling pathway by FRT was investigated. Our results showed that the expressions of Bax/Bcl-2, p-ERK/ ERK, p-p53/p53, and p-p38/p38 were decreased after FRT treatment compared with the radiation-treated group. FRT inhibited PARP-1 activation to inhibit AIF translocation from mitochondrion to nucleus. Pretreatment with FRT diminished the comet's tail and reduced fragments in six Gy-irradiated thymocytes compared with the irradiated cells without FRT treatment. We conclude that FRT enhanced radioprotection at least partially by regulating PARP-1/AIF to reduce apoptosis. J. Cell. Biochem. 118: 3943-3952, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Departmentof Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Ping
authors.identifier: 0000-0001-9819-6870
authors.initials: P
authors.lastname: Xu
authors.affiliation: The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Xingxia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Xiwen
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Synthetic Biology Remaking Engineering and Application Laboratory, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Departmentof Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Cai
authors.affiliation: Departmentof Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Qiu
authors.affiliation: Departmentof Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Hao
authors.affiliation: Departmentof Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Academy of Military Medical Sciences, Institute of Radiation Medicine, Beijing, 100850, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Lu
authors.affiliation: Synthetic Biology Remaking Engineering and Application Laboratory, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Departmentof Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Wancai
authors.initials: W
authors.lastname: Yang
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism* / Q000473:pathology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D051379:Mice; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000378:metabolism*; D027826:Rosa / Q000737:chemistry*
chemical_list: D051033:Apoptosis Inducing Factor; D005419:Flavonoids; C494830:AIFM1 protein, mouse; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1
keywords: AIF; APOPTOSIS; FRT; PARP-1; THYMUS CELLS