title: Ultrasound-assisted extraction and structural characterization by NMR of alginates and carrageenans from seaweeds.
abstract: Polysaccharides from seaweeds are interesting materials for food and pharmaceutical applications such as drug delivery due to their biocompatibility and biodegradability. Extraction of these biopolymers is usually performed during several hours to obtain a significant extraction yield. In this paper, we report on a new process to extract alginates from brown seaweeds (Sargassum binderi and Turbinaria ornata) and carrageenans from red seaweeds (Kappaphycus alvarezii and Euchema denticulatum) with the assistance of ultrasound. The effect of several parameters (pH, temperature, algae/water ratio, ultrasound power and duration) was investigated to determine optimal extraction conditions. The extracted polysaccharides represented up to 55% of the seaweeds dry weight and were obtained in a short time (15-30min) as compared to 27% in 2h for conventional extraction. NMR, FTIR and SEC analysis were used to characterise the extracted polymers. Ultrasound allowed the reduction of extraction time without affecting the chemical structure and molar mass distribution of alginates and carrageenans.
authors.affiliation: UMR DéTROI, Inserm U1188 - Université de la Réunion, Diabète atherohrombose Thérapies Réunion Océan Indien, CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France.
authors.forename: Latufa
authors.initials: L
authors.lastname: Youssouf
authors.affiliation: Plateforme de recherche CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Lallemand
authors.affiliation: UMR DéTROI, Inserm U1188 - Université de la Réunion, Diabète atherohrombose Thérapies Réunion Océan Indien, CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Giraud
authors.affiliation: UMR DéTROI, Inserm U1188 - Université de la Réunion, Diabète atherohrombose Thérapies Réunion Océan Indien, CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France.
authors.forename: Faiza
authors.initials: F
authors.lastname: Soulé
authors.affiliation: Centre for Biomedical and Biomaterials Research (CBBR), University of Mauritius, MSIRI Building, Réduit, Mauritius.
authors.forename: Archana
authors.initials: A
authors.lastname: Bhaw-Luximon
authors.affiliation: UMR DéTROI, Inserm U1188 - Université de la Réunion, Diabète atherohrombose Thérapies Réunion Océan Indien, CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France; CHU de La Réunion, Saint-Denis de La Réunion, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Meilhac
authors.affiliation: UMR DéTROI, Inserm U1188 - Université de la Réunion, Diabète atherohrombose Thérapies Réunion Océan Indien, CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France.
authors.forename: Christian Lefèbvre
authors.initials: CL
authors.lastname: D'Hellencourt
authors.affiliation: Centre for Biomedical and Biomaterials Research (CBBR), University of Mauritius, MSIRI Building, Réduit, Mauritius.
authors.forename: Dhanjay
authors.initials: D
authors.lastname: Jhurry
authors.affiliation: UMR DéTROI, Inserm U1188 - Université de la Réunion, Diabète atherohrombose Thérapies Réunion Océan Indien, CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France. Electronic address: joel.couprie@univ-reunion.fr.
authors.forename: Joël
authors.initials: J
authors.lastname: Couprie
mesh_terms: D000464:Alginates / Q000302:isolation & purification*; D002351:Carrageenan / Q000302:isolation & purification*; D000461:Rhodophyta / Q000737:chemistry; D044647:Sargassum / Q000737:chemistry; D012624:Seaweed / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D000464:Alginates; D002351:Carrageenan
keywords: Alginates; Carrageenans; Extraction; NMR; SEC; Ultrasound