title: MicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targetting EIF4G2.
abstract: Osteosarcoma (OS) is an aggressive malignant mesenchymal neoplasm amongst adolescents. The aim of the present study was to explore the various modes of action thatmiR-379has on the proliferation, migration, and invasion of human OS cells.miR-379achieves this by targetting eukaryotic initiation factor 4GII (EIF4G2). Human OS cell lines U2OS and MG-63 were selected and assigned into blank,miR-379mimics,miR-379mimic negative control (NC),miR-379inhibitors,miR-379inhibitor NC, EIF4G2 shRNA, control shRNA, andmiR-379inhibitor + EIF4G2 shRNA group. ThemiR-379expression andEIF4G2mRNA expression were detected utilising quantitative real-time PCR (qRT-PCR) and the EIF4G2 protein expression using Western blotting. MTT assay, scratch test, Transwell assay, and flow cytometry were performed to determine the proliferation, migration, invasion, and cell cycle, respectively. In comparison with themiR-379mimic NC group, themiR-379mimics group had decreased EIF4G2 expression; themiR-379inhibitors group indicated an increased EIF4G2 expression. Compared with the control shRNA group, the EIF4G2 expression was lower in the EIF4G2 shRNA group and themiR-379expression was dropped in themiR-379inhibitor + EIF4G2 shRNA group. The proliferation, migration, and invasion abilities of OS cells were reduced in themiR-379mimics and EIF4G2 shRNA groups. The percentage of OS cells at the G0/G1stage was increased, and the percentage at the S-stage was decreased in themiR-379mimics and EIF4G2 shRNA groups.miR-379may inhibit the proliferation, migration and invasion of OS cells through the down-regulation of EIF4G2.
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.|Department of Day Surgery Center, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China liyusheng0731@sina.com luowei0928@126.com.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Xiao
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Zhen-Han
authors.initials: ZH
authors.lastname: Deng
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: He
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China liyusheng0731@sina.com luowei0928@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics*; D016193:G1 Phase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D016192:Resting Phase, Cell Cycle / Q000235:genetics
chemical_list: C491300:EIF4G2 protein, human; D039603:Eukaryotic Initiation Factor-4G; C561022:MIRN379 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering
keywords: EIF4G2; Invasion; MicroRNA-379; Migration; Osteosarcoma; Proliferation