title: Low doses of bisphenol A can impair postnatal testicular development directly, without affecting hormonal or oxidative stress levels.
abstract: Bisphenol A (BPA) is considered a potent endocrine disruptor, causing changes in the endocrine system due to its oestrogenic activity. Male individuals may be susceptible to endocrine, morphological and physiological alterations during testicular postnatal development. The aim of the present study was to evaluate whether exposure to BPA during the peripubertal period can damage testicular development. To this end, male Wistar rats were treated with BPA via gavage at doses of 20 or 200µgkg-1on Postnatal Days (PND) 36-66. The control group was treated with Oil+DMSO under the same conditions. On PND 67, rats were killed. The blood was collected for hormonal analysis, the testis for sperm count, oxidative stress, histopathological and immunohistochemical analyses for ki-67 and sperm of the vas deferens for morphological analysis. Both doses of BPA resulted in abnormal sperm morphology and seminiferous tubules, with the highest dose increasing the height of the germinal epithelium and reducing the number of spermatozoa at Stages IX-XIII of spermatogenesis. In conclusion, both doses of BPA administered during the peripubertal period impaired testicular development without any effects on hormone levels (luteinizing hormone (LH), follicle stimulating hormone (FSH) and testosterone levels) or oxidative stress.
authors.affiliation: Department of General Biology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 - Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Ogo
authors.affiliation: Department of General Biology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 - Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Glaucia E M L
authors.initials: GEML
authors.lastname: Siervo
authors.affiliation: Department of General Biology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 - Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Géssica D
authors.initials: GD
authors.lastname: Gonçalves
authors.affiliation: Department of General Pathology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 - Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Rubens
authors.initials: R
authors.lastname: Cecchini
authors.affiliation: Department of General Pathology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 - Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Flavia A
authors.initials: FA
authors.lastname: Guarnier
authors.affiliation: Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, FORP, Av. do Café - S/N, 14040-904, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Janete Ap
authors.initials: JA
authors.lastname: Anselmo-Franci
authors.affiliation: Department of General Biology, Biological Sciences Center, State University of Londrina, Rodovia Celso Garcia Cid, PR 445 - Km 380, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
authors.forename: Glaura S A
authors.initials: GSA
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000254:growth & development; D013739:Testosterone / Q000097:blood; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D005640:Follicle Stimulating Hormone; D007986:Luteinizing Hormone; D010636:Phenols; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds