title: [Three-dimensional morphology of C6/36 cells infected by dengue virus: a study based on digital holographic microscopy].
abstract: To monitor the 3-dimensional (3D) morphological changes of C6/36 cells during dengue virus (DENV) infection using a live-cell imaging technique based on digital holographic microscopy and provide clues for better understanding the mechanisms of DENV infection.
  
  
  
  C6/36 cells were seeded in 6-well plates to determine the optimal imaging density under a holographic cell imager, and the morphological changes of the cells were recorded in response to a culture temperature change from 28 degrees celsius; to 37 degrees celsius; C6/36 cells were infected with 4 DENV strains with different serotypes at 28 degrees celsius; and incubated at 37 degrees celsius; for 24 h, and the 3D holograms and relevant morphological parameters were recorded at different time points using HoloMonitor M4 holographic cell imaging and analysis system.
  
  
  
  The holograms of C6/36 cells inoculated at the optimal density for imaging (4×105per well) showed unified 3D morphologies of the single cells with minimal dispersions in the cell area, thickness and volume (P<0.05), which did not undergo obvious changes when the cells were incubated at 37 degrees celsius; for 24 h (P>0.05). The cell area and volume of the cells infected with the 4 DENV strains all increased and the cell thickness was reduced during incubation. Among the 4 strains, DENV-1 and DENV-2 caused reduced cell thickness while DENV-3 and DENV-4 increased the cell thickness, and the pattern and degree of such changes differ among the 4 strains.
  
  
  
  Digital holographic microscopy allows monitoring of the complex morphological changes of cells during DENV infection. The 4 DENV strains with different serotypes causes characteristic cell damages during infection.
authors.affiliation: Biosafety Level 3 Laboratory, School of Public Health, Southern Medical University, Guangzhou 510515, China.E-mail: chienhai@163.com.
authors.forename: Jian-Hai
authors.initials: JH
authors.lastname: Yu
authors.forename: Xu-Ling
authors.initials: XL
authors.lastname: Liu
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Liu
authors.forename: Xiao-En
authors.initials: XE
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hui
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000330:Aedes / Q000166:cytology*; D000818:Animals; D002460:Cell Line / Q000648:ultrastructure* / Q000821:virology; D003716:Dengue Virus; D008853:Microscopy