title: Simultaneous quantification of 11 active constituents in Shexiang Baoxin Pill by ultraperformance convergence chromatography combined with tandem mass spectrometry.
abstract: On account of the complexity of chemical constituents of Shexiang Baoxin Pill (SBP), a famous traditional Chinese medicine (TCM) formula, a novel and effective UPC2-MS/MS method was developed to simultaneously determine the content of 11 active compounds of SBP with outstanding separation ability. Eleven components in SBP, including 2 ginsenosides, 2 bile acids, 3 bufadienolides and 4 volatiles were detected by electrospray ionization tandem mass spectrometry in positive and negative ion modes with multiple reaction monitor (MRM). The analysis was performed at 30°C using an Acquity UPC2Diol (3.0×50mm, 1.7μm) column with linear gradient elution (eluent A, CO2; eluent B, methanol containing 20mM ammonium acetate), back pressure of 2000 psi, flow rate of 1.2mL/min and the injection volume of 1.0μL. The method was extensively validated regarding the linearity (r≥0.9974), precision (≤3.11%), recovery (93.34-104.50%), repeatability (≤2.00%) and stability (≤4.20%). Using this method, 11 active compounds of SBP with different polarity were simultaneously quantified in one chromatography analysis within 8min. Statistical analysis of the effects of 11 compounds on the quality of SBP revealed that the content of cinnamaldehyde varied widely in different batches. This work presents an exemplary study for quality control of complex samples, especially for TCMs.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Department of Nephrology, the 455th Hospital of PLA, Shanghai 200052, PR China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Niao
authors.initials: N
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Waters Corporation, Building 13, No. 1000 JinHai Road, Pudong New District, Shanghai 201206, PR China.
authors.forename: Yongwei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Waters Corporation, Building 13, No. 1000 JinHai Road, Pudong New District, Shanghai 201206, PR China.
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Nephrology, the 455th Hospital of PLA, Shanghai 200052, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Shanghai Hutchison Pharmaceuticals Company, Shanghai 200331, PR China.
authors.forename: Changsen
authors.initials: C
authors.lastname: Zhan
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, PR China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address: lyliurh@126.com.
authors.forename: Runhui
authors.initials: R
authors.lastname: Liu
mesh_terms: D001647:Bile Acids and Salts / Q000032:analysis; D002018:Bufanolides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D036145:Ginsenosides / Q000032:analysis; D057230:Limit of Detection; D011786:Quality Control; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D001647:Bile Acids and Salts; D002018:Bufanolides; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D055549:Volatile Organic Compounds; C526712:shexiang baoxin
keywords: Non-volatiles; Quantification; Shexiang Baoxin Pill; UPC(2)-MS/MS; Volatiles