title: Effectiveness of Photodynamic Therapy in Elimination of HPV-16 and HPV-18 Associated with CIN I in Mexican Women.
abstract: This study aimed to determine the effectiveness of photodynamic therapy (PDT), using δ-aminolevulinic acid (5-ALA), in the elimination of premalignant cervical lesions in Mexican patients with human papillomavirus (HPV) infection and/or cervical intraepithelial neoplasia (CIN). Thirty women diagnosed with CIN I and/or positive for HPV participated in the study. Topical 6% 5-ALA in gel form was applied to the uterine cervix; after 4 h, the lesion area was irradiated with a light dose of 200 J cm-2at 635 nm. This procedure was performed three times at 48-h intervals. Clinical follow-up was performed at 3, 6, and 12 months after the initial PDT administration, by colposcopy, cervical cytology, histopathological analysis, polymerase chain reaction, and hybrid capture. Of HPV-infected patients without evidence of CIN I, 80% cleared the infection, while HPV associated with CIN I was eliminated in 83% of patients (P < 0.05). At 12 months, CIN I had regressed in 57% of patients, although this response was not statistically significant. PDT using 6% 5-ALA is concluded to be effective in eliminating HPV infection associated or not with CIN I.
authors.affiliation: Laboratorio de Citopatología Ambiental, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Ciudad de México, México.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Maldonado Alvarado
authors.affiliation: Instituto Estatal de Cancerología de Guerrero "Dr. Arturo Beltrán Ortega", Acapulco Guerrero, México.
authors.forename: Martha Olivia
authors.initials: MO
authors.lastname: Osorio Peralta
authors.affiliation: Departamento de Patología, Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Ciudad de México, México.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Moreno Vázquez
authors.affiliation: Centro de Salud T-III México-España, Ciudad de México, México.
authors.forename: Lydia Alejandra
authors.initials: LA
authors.lastname: Martínez Guzmán
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Maria Eugenia
authors.initials: ME
authors.lastname: Melo Petrone
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Zulma Iveth
authors.initials: ZI
authors.lastname: Enriquez Mar
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Dora Estela
authors.initials: DE
authors.lastname: Jovel Galdamez
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Carrión Solana
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Balderas Martínez
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Parra
authors.affiliation: Comité para la Educación y Desarrollo Integral de la Mujer A.C., San Andrés Tuxtla, Veracruz, México.
authors.forename: Rossana Inés
authors.initials: RI
authors.lastname: Castellanos Oliveros
authors.affiliation: Laboratorio de Citopatología Ambiental, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Ciudad de México, México.
authors.forename: Rosa Linda
authors.initials: RL
authors.lastname: Bello Leiva
authors.affiliation: Clínica de Displasias, Hospital Regional "Gral. Ignacio Zaragoza" ISSSTE, Ciudad de México, México.
authors.forename: Araceli
authors.initials: A
authors.lastname: Espinosa Montesinos
authors.affiliation: Laboratorio de Citopatología Ambiental, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Ciudad de México, México.
authors.forename: Citlalli
authors.initials: C
authors.lastname: Barrera Mendoza
authors.affiliation: Laboratorio de Citopatología Ambiental, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Ciudad de México, México.
authors.forename: Selma Eugenia
authors.initials: SE
authors.lastname: Medina García
authors.affiliation: Laboratorio de Citopatología Ambiental, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Ciudad de México, México.
authors.forename: Eva
authors.initials: E
authors.lastname: Ramón Gallegos
mesh_terms: D000328:Adult; D000622:Aminolevulinic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D052162:Human papillomavirus 16 / Q000187:drug effects*; D052161:Human papillomavirus 18 / Q000187:drug effects*; D006801:Humans; D008800:Mexico; D030361:Papillomavirus Infections / Q000188:drug therapy* / Q000821:virology*; D010778:Photochemotherapy / Q000009:adverse effects*; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D016896:Treatment Outcome; D002578:Uterine Cervical Dysplasia / Q000188:drug therapy* / Q000821:virology
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid