title: Prospecting fungal parasites of the potato cyst nematode Globodera pallida using a rapid screening technique.
abstract: Seven filamentous fungal species were isolated from individual eggs of Globodera pallida cysts collected from infested fields in Shelley Idaho, USA and identified as Chaetomium globosum, Fusarium oxysporum, Fusarium solani, Fusarium tricinctum, Microdochium bolleyi, Purpureocillium lilacinum, and Plectosphaerella cucumerina. Their ability to reduce infection by G. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-ROCs). All fungi reduced G. pallida infection in potato, but greatest reduction was observed with C. globosum at an average reduction of 76%. Further non-destructive methods were developed to rapidly assess biological control potential of putative fungal strains by staining the infectious second stage juveniles of G. pallida with the live fluorescent stain PKH26. In comparisons between the standard, invasive acid fuchsin method and use of the live stain PKH26, no significant difference in infection level of G. pallida was observed whether roots were stained with PKH26 or acid fuchsin. For both methods, a similar reduction (77% for acid fuchsin, and 78% for PKH26 stain) in invasion of infectious stage of G. pallida was observed when potato plants were inoculated with C. globosum compared to non-inoculated potato.
authors.affiliation: Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow, Idaho.
authors.forename: Rinu
authors.identifier: http://orcid.org/0000-0002-0127-4674
authors.initials: R
authors.lastname: Kooliyottil
authors.affiliation: Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow, Idaho.
authors.forename: Louise-Marie
authors.identifier: http://orcid.org/0000-0003-2689-2548
authors.initials: LM
authors.lastname: Dandurand
authors.affiliation: Soil and Land Resources Division, University of Idaho, Moscow, Idaho.
authors.forename: Guy R
authors.initials: GR
authors.lastname: Knudsen
mesh_terms: D000818:Animals; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009930:Organic Chemicals; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology* / Q000469:parasitology*; D018517:Plant Roots / Q000382:microbiology / Q000469:parasitology; D058441:Rhizosphere; D012987:Soil / Q000469:parasitology; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000382:microbiology* / Q000469:parasitology*; D014431:Tylenchoidea / Q000382:microbiology*
chemical_list: D009930:Organic Chemicals; C070080:PKH 26; D012987:Soil
keywords: Globodera pallida; biocontrol agent; fungal antagonist; nematophagous fungi; potato cyst nematode