title: DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells.
abstract: The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Harterink
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Marta Esteves
authors.initials: ME
authors.lastname: da Silva
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Lena
authors.initials: L
authors.lastname: Will
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Turan
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Adiljan
authors.initials: A
authors.lastname: Ibrahim
authors.affiliation: Institute for Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs University of Freiburg, Freiburg, Germany.
authors.forename: Alexander E
authors.initials: AE
authors.lastname: Lang
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands.
authors.forename: Eljo Y
authors.initials: EY
authors.lastname: van Battum
authors.affiliation: Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands.
authors.forename: R Jeroen
authors.identifier: http://orcid.org/0000-0003-1631-6440
authors.initials: RJ
authors.lastname: Pasterkamp
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Lukas C
authors.initials: LC
authors.lastname: Kapitein
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Kudryashov
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Ben A
authors.initials: BA
authors.lastname: Barres
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Casper C
authors.initials: CC
authors.lastname: Hoogenraad
authors.affiliation: Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.|Department of Neurosurgery, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: J Bradley
authors.identifier: http://orcid.org/0000-0002-4706-2538
authors.initials: JB
authors.lastname: Zuchero
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D018260:Gelsolin / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D010455:Peptides / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014162:Transfection; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000199:Actins; D018260:Gelsolin; D010455:Peptides; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D049452:Green Fluorescent Proteins; D036002:ADP Ribose Transferases; C415223:spvB protein, Salmonella enterica virulence plasmid