title: Dcf1 regulates neuropeptide expression and maintains energy balance.
abstract: Neuropeptide Y (NPY) is an important neurotransmitter in the brain that plays a pivotal role in food intake and energy storage. Although many studies have focused on these functions, the regulation of NPY expression remains unclear. Here we showed that dendritic cell factor 1 (Dcf1) regulates NPY expression and maintains energy balance. We found that NPY expression is significantly reduced in the hypothalamus of Dcf1 knockout (Dcf1-/-, KO) mice. In contrast, Dcf1 overexpression significantly increases NPY expression in the cell line. We also found that Dcf1 acts upstream of the NPY gene to regulate NPY expression and modulates the NPY-NPY receptor 1-GABA signal. Notably, we observed a significant increase in the ATP concentration in Dcf1-/-mice, suggesting a greater demand for energy in the absence of Dcf1. We studied the relationship between Dcf1 and NPY and revealed that Dcf1 plays a critical role in energy balance.
authors.affiliation: Laboratory of Molecular Neural Biology, School of Life Science, Shanghai University, 333 Nanchen Road, Shanghai, 200444, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Neural Biology, School of Life Science, Shanghai University, 333 Nanchen Road, Shanghai, 200444, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Laboratory of Molecular Neural Biology, School of Life Science, Shanghai University, 333 Nanchen Road, Shanghai, 200444, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Neural Biology, School of Life Science, Shanghai University, 333 Nanchen Road, Shanghai, 200444, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: Laboratory of Molecular Neural Biology, School of Life Science, Shanghai University, 333 Nanchen Road, Shanghai, 200444, China. Electronic address: wtq@shu.edu.cn.
authors.forename: Tieqiao
authors.initials: T
authors.lastname: Wen
mesh_terms: D000818:Animals; D004435:Eating / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D014018:Tissue Distribution; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D005680:gamma-Aminobutyric Acid; C000626540:Tmem59 protein, mouse; C000606148:TMEM59 protein, human
keywords: Dcf1; GABA; NPY; NPY-Y1; energy balance