title: Capillary electrophoresis method for the analysis of organic acids and amino acids in the presence of strongly alternating concentrations of aqueous lactic acid.
abstract: During the production of bio-based bulk chemicals, such as lactic acid (LA), organic impurities have to be removed to produce a ready-to-market product. A capillary electrophoresis method for the simultaneous detection of LA and organic impurities in less than 10 min was developed. LA and organic impurities were detected using a direct UV detection method with micellar background electrolyte, which consisted of borate and sodium dodecyl sulfate. We investigated the effects of electrolyte composition and temperature on the speed, sensitivity, and robustness of the separation. A few validation parameters, such as linearity, limit of detection, and internal and external standards, were evaluated under optimized conditions. The method was applied for the detection of LA and organic impurities, including tyrosine, phenylalanine, and pyroglutamic acid, in samples from a continuous LA fermentation process from post-extraction tapioca starch and yeast extract.
authors.affiliation: Department of Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469, Potsdam, Germany. hendrik_laube@yahoo.de.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Laube
authors.affiliation: ICA Boden-Haumann-Mainka, Engineering Society for Chemical Analysis, Langen, Hessen, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Boden
authors.affiliation: Department of Bioengineering, Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V., Max-Eyth-Allee 100, 14469, Potsdam, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Schneider
mesh_terms: D000596:Amino Acids; D019075:Electrophoresis, Capillary; D019344:Lactic Acid / Q000032:analysis*
chemical_list: D000596:Amino Acids; D019344:Lactic Acid
keywords: Amino acids; Fermentation broth; Lactic acid; Organic acids; Process monitoring; Pyroglutamic acid