title: RAPD assisted selection of black gram (Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm.
abstract: Black gram (Vigna mungo L. Hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. The yield potential of Black gram is shallow due to lack of genetic variability and biotic stress susceptibility. Core biotic stress factors include mung bean yellow mosaic virus (MYMV), urdbean leaf crinkle virus (UCLV), wilt (Fusarium oxysporum) and powdery mildew (Erysiphe polygoni DC). Although many studies determine resistant varieties to a particular disease, however, it is often complimented by low yield and susceptibility to other diseases. Hence, this study focuses on investigating the genetic relationships among three varieties and nine accessions of black gram having disease resistance to previously described diseases and susceptibility using random amplified polymorphic deoxyribonucleic acid (RAPD) markers. A total of 33 RAPD primers were used for diversity analysis and yielded 206 fragments. Number of amplified fragments ranged from two (OPN-1) to 13 (OPF-1). The highest similarity coefficient was observed between IC-145202 and IC-164118 (0.921), while lowest similarity was between PU-31 and IC-145202 (0.572). The genetic diversity obtained in this study along with disease analysis suggests PU31as a useful variety for the development of markers linked to MYMV, UCLV, wilt and powdery mildew resistance by marker-assisted back cross breeding and facilitates the production of crosses with multiple disease resistance.
authors.affiliation: Biotechnology Section, Crop Improvement Division, Indian Institute of Rice Research (ICAR-IIRR), Hyderabad, India.
authors.forename: B
authors.initials: B
authors.lastname: Vishalakshi
authors.affiliation: Biotechnology Section, Crop Improvement Division, Indian Institute of Rice Research (ICAR-IIRR), Hyderabad, India.
authors.forename: B
authors.initials: B
authors.lastname: Umakanth
authors.affiliation: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre (BARC), Mumbai, India. anirudh.skylark@gmail.com.
authors.forename: Anirudh P
authors.initials: AP
authors.lastname: Shanbhag
authors.affiliation: Biomoneta Research Private Limited, Centre for Cellular and Molecular Platforms, NCBS, (TIFR), Bengaluru, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Ghatak
authors.affiliation: National Center for Biological Sciences (NCBS), TIFR, Bengaluru, India.
authors.forename: Nitish
authors.initials: N
authors.lastname: Sathyanarayanan
authors.affiliation: Biotechnology Section, Crop Improvement Division, Indian Institute of Rice Research (ICAR-IIRR), Hyderabad, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Madhav
authors.affiliation: Agri Biotech Foundation, Rajendra Nagar, Hyderabad, India.
authors.forename: G Gopala
authors.initials: GG
authors.lastname: Krishna
authors.affiliation: Regional Research Station (RARS-PJTSAU), Warangal, India.
authors.forename: Hari
authors.initials: H
authors.lastname: Yadla
keywords: Mung bean yellow mosaic virus (MYMV); Powdery mildew; Rapid amplification of polymorphic DNA (RAPD); Urdbean leaf crinkle virus (UCLV); Vigna mungo L. Hepper; Wilt