title: Low-Frequency Dynamics of BSA Complementarily Studied by Raman and Inelastic Neutron Spectroscopy.
abstract: The present study focuses on protein motions on the picosecond time scale, generally characterized by the overlapping of vibrational and relaxational dynamics in disordered molecular systems. Recently, it has been demonstrated that a dry protein, bovine serum albumin (BSA), shows a glass-like transition in the temperature range between 240 and 260 K. Here, we present the results of combined low-frequency Raman and inelastic neutron scattering studies of dry BSA under conditions similar to those of this glass-like transition. The use of both techniques allows us to perform a detailed comparison of the dynamic susceptibility and vibrational density of states of BSA obtained at different temperatures and to calculate the light-vibration coupling coefficient, C(ω). Moreover, we analyzed the temperature evolution of the boson peak and a peak located at ∼80 cm-1, which has previously been identified to originate from protein dynamics, and observed that both modes show anomalous temperature behavior in the vicinity of Tg.
authors.affiliation: Jülich Center for Neutron Science (JCNS), Forschungszentrum Jülich GmbH , Outstation at MLZ, Lichtenbergstraße 1, 85747 Garching, Germany.|A.F. Ioffe Physical Technical Institute , ul. Politekhnicheskaya 26, 194021 St. Petersburg, Russian Federation.
authors.forename: Anna V
authors.identifier: 0000-0002-6820-3731
authors.initials: AV
authors.lastname: Frontzek Neé Svanidze
authors.affiliation: Laboratory for Neutron Scattering and Imaging, Paul Scherrer Institut , CH-5232 Villigen, Switzerland.
authors.forename: Jan Peter
authors.identifier: 0000-0002-2816-2875
authors.initials: JP
authors.lastname: Embs
authors.affiliation: Université Lille Nord de France , F-59000 Lille, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Paccou
authors.affiliation: Université Lille Nord de France , F-59000 Lille, France.|USTL UMET UMR CNRS 8207 , F-59655 Villeneuve d'Ascq, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Guinet
authors.affiliation: Université Lille Nord de France , F-59000 Lille, France.|USTL UMET UMR CNRS 8207 , F-59655 Villeneuve d'Ascq, France.
authors.forename: Alain
authors.identifier: 0000-0002-8244-3512
authors.initials: A
authors.lastname: Hédoux
mesh_terms: D000818:Animals; D002417:Cattle; D056004:Molecular Dynamics Simulation; D033363:Neutron Diffraction; D009502:Neutrons; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013696:Temperature; D014732:Vibration
chemical_list: D012710:Serum Albumin, Bovine