title: IL-17 and IFN-γ producing NK and γδ-T cells are preferentially expanded in synovial fluid of patients with reactive arthritis and undifferentiated spondyloarthritis.
abstract: The IL-17/1L-23 axis is important in the pathogenesis of spondyloarthropathy. Innate cells produce IL-17 in addition to Th17 cells. We studied the frequencies of natural killer (NK) (total, CD56bright, CD56dim, perforin+ and granzyme+), NK-T, γδ-T, and IFN-γ+, IL-17+ NK and γδ-T cells in peripheral blood (PB) and synovial fluid (SF) of ReA/uSpA patients. PB from 45 patients and paired SF from 39 patients were studied, together with PB from 18 healthy controls (HC). The frequency of γδ-T cells was decreased (p<0.05) while IL-17 producing NK and γδ-T cells were increased (p<0.05) in PB of patients as compared to HC. In SF, CD56brightNK cells were increased (p<0.001) but had reduced expression of perforin and granzyme (p<0.0001) as compared to PB. Frequency of IL-17+, IFN-γ+ NK and γδ-T cells was higher in SF as compared to PB (p<0.05). We suggest that innate cells by producing pro-inflammatory cytokines may contribute to pathogenesis.
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India.
authors.forename: Abhra Chandra
authors.initials: AC
authors.lastname: Chowdhury
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India.
authors.forename: Smriti
authors.initials: S
authors.lastname: Chaurasia
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India.
authors.forename: Shravan Kumar
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Aggarwal
authors.affiliation: Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow Pin: 226014, India. Electronic address: rnmisra@sgpgi.ac.in.
authors.forename: Ramnath
authors.initials: R
authors.lastname: Misra
mesh_terms: D000293:Adolescent; D000328:Adult; D016918:Arthritis, Reactive / Q000473:pathology; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D008297:Male; D000091402:Prohibitins; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D025242:Spondylarthropathies / Q000473:pathology*; D013582:Synovial Fluid; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D020381:Interleukin-17; C000717331:PHB2 protein, human; D000091402:Prohibitins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma
keywords: Gamma Delta T cells; Inflammation; Interleukin 17; Natural killer cells; Synovial fluid