title: Hyaluronic acid and beta cyclodextrins films for the release of corneal epithelial cells and dexamethasone.
abstract: In this work we prepared hydrogels based on hyaluronic acid and β-cyclodextrins to sustain the release of both corneal epithelial cells and dexamethasone. This steroid is administered as eye drops several times per day to reduce the risk of rejection in the post operative period after the cornea transplantation and cell release techniques. Hydrogels were produced by crosslinking an amino derivative of hyaluronic acid, with the divinyl sulfone derivative of β-cyclodextrins, this last employed as a crosslinker and solubilizing agent. Drug release studies revealed that dexamethasone containing samples are able to extend the release of this drug for at least five days. Biological studies, conducted with human corneal epithelial cells, showed that it is possible to employ the hydrogels for the temporary seeding of the cells and their potential release onto the cornea.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: calogero.fiorica@unipa.it.
authors.forename: Calogero
authors.initials: C
authors.lastname: Fiorica
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Fabio Salvatore
authors.initials: FS
authors.lastname: Palumbo
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pitarresi
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Bongiovì
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy; Mediterranean Center for Human Advanced Biotechnologies (Med-Chab), Viale delle Scienze Ed.18, 90128 Palermo, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology; D003907:Dexamethasone / Q000008:administration & dosage*; D004337:Drug Carriers; D065546:Drug Liberation; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D047392:beta-Cyclodextrins; D003907:Dexamethasone; D006820:Hyaluronic Acid
keywords: Cell release systems; Corneal wounds; Dexamethasone; Hyaluronic acid hydrogels