title: The effects of chrysophanol on ovalbumin (OVA)-induced chronic lung toxicology by inhibiting Th17 response.
abstract: Chrysophanol (CH), extracted from plants of Rheum genus, possesses various pharmacological effects including anti-inflammatory activity. The purpose of the present study was to evaluate the protective effects and the underlying mechanisms of CH on ovalbumin (OVA)-induced asthma in mice. Fifty mice were randomly assigned to five experimental groups: control group, model group, dexamethasone (2 mg/kg) group and CH (5 and 10 mg/kg) groups. The number of eosinophil cells and the production of interleukin-6 (IL-6), IL-1β, IL-17 A and tumor necrosis factor-α in bronchoalveolar lavage fluid (BALF) were measured. In addition, pulmonary histopathology, airway resistance (Raw), T-helper17 (Th17) cells frequency and RORγt expression were evaluated. Our study demonstrated that CH effectively decreased eosinophil count and inflammatory cytokines production in BALF. In addition, treatment with CH significantly inhibited the Raw, Th17 percentage and RORγt expression in OVA-induced animals compared with those in model group. Histological studies also demonstrated that CH significantly suppressed OVA-induced eosinophilia in lung tissue compared with model group. Our findings supported that CH can prevent allergic asthma in the mouse model.
authors.affiliation: a Department of Cardiology , the Second Affiliated Hospital, Medical School of Xi?an Jiaotong University , Xi'an , P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a Department of Cardiology , the Second Affiliated Hospital, Medical School of Xi?an Jiaotong University , Xi'an , P.R. China.
authors.forename: Yafan
authors.initials: Y
authors.lastname: Song
authors.affiliation: a Department of Cardiology , the Second Affiliated Hospital, Medical School of Xi?an Jiaotong University , Xi'an , P.R. China.
authors.forename: Congxia
authors.initials: C
authors.lastname: Wang
authors.affiliation: a Department of Cardiology , the Second Affiliated Hospital, Medical School of Xi?an Jiaotong University , Xi'an , P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: b Department of Oncology, the Second Affiliated Hospital , Medical School of Xi'an Jiaotong University , Xi'an , P.R. China.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Kang
authors.affiliation: b Department of Oncology, the Second Affiliated Hospital , Medical School of Xi'an Jiaotong University , Xi'an , P.R. China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Ma
authors.affiliation: c Changchun University of Chinese Medicine , Changchun , P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: c Changchun University of Chinese Medicine , Changchun , P.R. China.
authors.forename: Tianzhu
authors.initials: T
authors.lastname: Zhang
authors.affiliation: c Changchun University of Chinese Medicine , Changchun , P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Shumin
authors.affiliation: c Changchun University of Chinese Medicine , Changchun , P.R. China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ma
mesh_terms: D000403:Airway Resistance / Q000187:drug effects / Q000276:immunology; D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D012250:Rheum / Q000737:chemistry; D058504:Th17 Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D000880:Anthraquinones; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010047:Ovalbumin; C027113:chrysophanic acid
keywords: Chrysophanol; RORγt; Th17 cell; lung toxicology