title: Simultaneous determination of pyrametostrobin and its two metabolites in cucumber and soil using a quick, easy, cheap, effective, rugged, and safe method with HPLC-MS/MS.
abstract: An easy, effective and sensitive analytical method for the simultaneous determination of a novel fungicide pyrametostrobin and its two metabolites pyrametostrobin-M1 and pyrametostrobin-M2 in cucumber and soil was developed using a quick, easy, cheap, effective, rugged, and safe method with high-performance liquid chromatography and tandem mass spectrometry. The extraction solvent was acetonitrile, and cleanup sorbents were primary secondary amine and graphitized carbon black for cucumber samples and primary secondary amine for soil samples. The three target compounds were successfully separated between 3.2 and 3.9 min using a Waters CORTECS™ C18column connected to an electrospray ionization source. All the matrix-matched samples at three fortified levels (10, 100 and 1000  μg/kg) provided satisfactory recoveries in the range of 78.8-93.8% with relative standard deviations below 6.9%. The limits of quantitation for the three compounds were below 0.183 μg/kg. The proposed method was validated by analyzing real samples.
authors.affiliation: College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. China.|Shenyang Research Institute of Chemical Industry, Shenyang, P. R. China.
authors.forename: Chengtian
authors.identifier: http://orcid.org/0000-0002-2793-7026
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. China.
authors.forename: He
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Shenyang Research Institute of Chemical Industry, Shenyang, P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Shenyang Research Institute of Chemical Industry, Shenyang, P. R. China.
authors.forename: Fuyan
authors.initials: F
authors.lastname: Jia
authors.affiliation: College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. China.
authors.forename: Bochui
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Shenyang Research Institute of Chemical Industry, Shenyang, P. R. China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wei
authors.affiliation: Shenyang Research Institute of Chemical Industry, Shenyang, P. R. China.
authors.forename: Dangsheng
authors.initials: D
authors.lastname: Gong
authors.affiliation: Shenyang Research Institute of Chemical Industry, Shenyang, P. R. China.
authors.forename: Weichang
authors.initials: W
authors.lastname: Xu
authors.affiliation: College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. China.
authors.forename: Mingshan
authors.initials: M
authors.lastname: Ji
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D012987:Soil / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014675:Vegetables / Q000737:chemistry*
chemical_list: D005659:Fungicides, Industrial; D012987:Soil
keywords: high-performance liquid chromatography; metabolites; multiresidue analysis; pyrametostrobin; tandem mass spectrometry