title: Multicenter Performance Assessment of Carba NP Test.
abstract: Eighty Gram-negative bacilli (54Enterobacteriaceaeand 26 nonfermenting Gram-negative bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P< 0.05). Previously described limitations withblaOXA-48-likecarbapenemases andblaOXAcarbapenemases associated withAcinetobacter baumanniiwere noted. Based on these data, we demonstrate that the Carba NP test, when implemented with the standardized CLSI methodology, provides reproducible results across multiple sites for detection of carbapenemases.
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Cunningham
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Brandi
authors.initials: B
authors.lastname: Limbago
authors.affiliation: Clinical Microbiology Institute, Wilsonville, Oregon, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Traczewski
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Anderson
authors.affiliation: International Health Management Associates, Inc., Schaumburg, Illinois, USA.
authors.forename: Meredith
authors.initials: M
authors.lastname: Hackel
authors.affiliation: University of California Los Angeles Medical Center, Los Angeles, California, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Hindler
authors.affiliation: International Health Management Associates, Inc., Schaumburg, Illinois, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Sahm
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Efe
authors.initials: E
authors.lastname: Alyanak
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Adrian
authors.initials: A
authors.lastname: Lawsin
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Gulvik
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Tom J B
authors.initials: TJB
authors.lastname: de Man
authors.affiliation: Divison of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Jayawant N
authors.initials: JN
authors.lastname: Mandrekar
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.|Weill Cornell Medical College/New York-Presbyterian Hospital, New York, New York, USA.
authors.forename: Audrey N
authors.initials: AN
authors.lastname: Schuetz
authors.affiliation: Weill Cornell Medical College/New York-Presbyterian Hospital, New York, New York, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Jenkins
authors.affiliation: University of California Los Angeles Medical Center, Los Angeles, California, USA.
authors.forename: Romney
authors.initials: R
authors.lastname: Humphries
authors.affiliation: Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Palavecino
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.|Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore, Republic of Singapore.|Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Shawn
authors.initials: S
authors.lastname: Vasoo
authors.affiliation: Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA patel.robin@mayo.edu.|Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014481:United States; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Gram-negative bacilli; antimicrobial resistance; carbapenemase; screening