title: Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy.
abstract: Diabetic nephropathy is a major cause of chronic kidney disease and end-stage kidney disease. However, so little is known about alterations of the glycopatterns in urine with the development of diabetic nephropathy. Presently, we interrogated glycopatterns in urine specimens using a lectin microarray. The results showed that expression levels of Siaα2-6Gal/GalNAc recognized by SNA exhibited significantly increased tendency with the development of diabetic nephropathy; moreover, SNA blotting indicated glycoproteins (90 kDa, 70 kDa, and 40 kDa) in urine may contribute to this alteration. Furthermore, the glycopatterns of (GlcNAc)2-4recognized by STL exhibited difference between diabetic and nondiabetic nephropathy. The results of urinary protein microarray fabricated by another 48 urine specimens also indicated (GlcNAc)2-4is a potential indictor to differentiate the patients with diabetic nephropathy from nondiabetic nephropathy. Furtherly, STL blotting showed that the 50 kDa glycoproteins were correlated with this alteration. In conclusion, our data provide pivotal information to monitor the development of diabetic nephropathy and distinguish between diabetic nephropathy and nondiabetic renal disease based on precise alterations of glycopatterns in urinary proteins, but further studies are needed in this regard.
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Hanyu
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Nephrology, General Hospital of Jinan Military Command, Jinan, China.
authors.forename: Moyan
authors.initials: M
authors.lastname: Liu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Hanjie
authors.identifier: 0000-0003-2684-8715
authors.initials: H
authors.lastname: Yu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Xiawei
authors.identifier: 0000-0001-5444-4692
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Xinle
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Guangyan
authors.initials: G
authors.lastname: Cai
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Geng
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Wu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Zheng
authors.identifier: 0000-0002-1839-3450
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, China.
authors.forename: Dong
authors.identifier: 0000-0001-5451-9877
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D001706:Biopsy; D003924:Diabetes Mellitus, Type 2 / Q000652:urine; D003928:Diabetic Nephropathies / Q000175:diagnosis* / Q000652:urine*; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000652:urine; D037102:Lectins / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D051436:Renal Insufficiency, Chronic / Q000652:urine; D046888:Tissue Array Analysis; D016482:Urinalysis / Q000379:methods*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D037102:Lectins