title: The Efficacy of Isolated Bacteriophages from Pig Farms against ESBL/AmpC-ProducingEscherichia colifrom Pig and Turkey Farms.
abstract: Extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases are plasmid (but also chromosomally) encoded enzymes found in Enterobacteriaceae, determining resistance to a variety of important antibiotics including penicillins, cephalosporins, and monobactams. In recent decades, the prevalence of ESBL/AmpC-producing bacteria has increased rapidly across the world. Here, we evaluate the potential use of bacteriophages in terms of a reduction of antibiotic-resistant bacteria in healthy animals. The aim of our studies was to isolate bacteriophages capable of destroying ESBL/AmpC-producingEscherichia coliisolated from livestock habitats. The efficacy of isolated phages against ESBL/AmpCE. colistrains varies, but creation of a phage cocktail with broad activity spectrum is possible. This may indicate that the role of phages may not be limited to phage therapy, but bacterial viruses may also be applied against spread of bacteria with antibiotic resistance genes in the environment. We also addressed the hypothesis, that phages, effective for therapeutic purposes may be isolated from distant places and even from different environments other than the actual location of the targeted bacteria. This may be beneficial for practical purposes, as the construction of effective phage preparations does not require access to disease outbreaks.
authors.affiliation: Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences Wrocław, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Skaradzińska
authors.affiliation: Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences Wrocław, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Śliwka
authors.affiliation: Department of Biotechnology and Food Microbiology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences Wrocław, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kuźmińska-Bajor
authors.affiliation: Department of Fermentation and Cereals Technology, Faculty of Food Science, Wrocław University of Environmental and Life Sciences Wrocław, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Skaradziński
authors.affiliation: Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Rząsa
authors.affiliation: Department of Veterinary Medicine, Institute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin Berlin, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Friese
authors.affiliation: Department of Veterinary Medicine, Institute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin Berlin, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Roschanski
authors.affiliation: Department of Veterinary Medicine, Institute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin Berlin, Germany.
authors.forename: Jayaseelan
authors.initials: J
authors.lastname: Murugaiyan
authors.affiliation: Department of Veterinary Medicine, Institute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin Berlin, Germany.
authors.forename: Uwe H
authors.initials: UH
authors.lastname: Roesler
keywords: ESBL/AmpC resistant E. coli; antibiotic resistance; antibiotic resistance genes; bacteriophages; phage therapy