title: Illumina HiSeq 2000 sequencing; 5â-tag sequencing analysis of mouse mitotic chromosome RNA
design: 5â-tag sequencing analysis of mouse mitotic chromosome RNA
library_construction_protocol: First, mitotic chromosomes were isolated from cells, and then after salt treatment RNA was extracted from these two mitotic chromosome samples. Mitotic chromosomes isolated by low salt PA buffer. '5'' end tag sequencing protocol'