title: GSM4597452: S2CK; Mus musculus; RNA-Seq
library_construction_protocol: Pituitaries were harvested, the posterior and the anterior lobes were removed and intermediate lobes isolated and incubated separately in a solution of papain  for 15 min at 37ºC in presence of 10ug/ml of both DNAse  and Rock Inhibitor. The papain solution was then removed, and mechanical dissociation was performed on ice in pituisphere medium. Library generation for 10x Genomics analysis were performed following the Chromium Single Cell 3′ Reagents Kits (10x Genomics) and sequenced on an Hiseq4000 (Illumina).