title: next gerenation RNA-seq
design: To achieve a cost-effective deep sequencing of the transcriptome, an equal aliquot of RNA from each of the nutrient conditions (nutrient-replete, -Cu, -Mn, Ni, + 1/5 Zn and + 1/5 Fe) was mixed to produce a mix > 5 g, then conducted Illumina RNA-Seq sequencing (10Gbp)