title: 16S RNA-Seq of upper gastrointestinal carcinoma microbiota
design: DNA was isolated from UGI tract biopsies and mucosal specimens by using the MOBIO PowerSoil DNA Isolation Kit. The V4 region of the 16S rRNA gene was amplified using the universal bacterial primer set of 515-Forward and 806-Reverse. Using Illumina V4 chemistry and paired-end 2150 bp reads, sequencing was performed on the Illumina MiniSeq platform. Original sequence data processing was performed by the Illumina MiniSeq Reporter to remove adapter and primer sequences and then sequence data was exported in the FASTQ format.