title: GSM4656050: Sample SWT1 from D melanogaster strain LUN-007 (Sensitive to desiccation), under Treated conditions; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit from 30 whole female flies, four to six days old, per replicate. RNA samples were treated with DNAse I (Thermo Fisher Scientific) following manufacturer's instructions. RNA concentration was checked with NanoDrop spectrophotometer (NanoDrop Technologies) and quality was assessed with Bioanalyzer. 1.5 µg of total RNA from each sample was used for library preparation and sequencing. Library preparation was performed using the Truseq Stranded mRNA Sample Prep kit from Illumina following the manufacturer's instructions. Libraries were sequenced using Illumina 125 bp paired-end reads, obtaining between 27 and 41 million reads/sample.