title: GSM2719416: CT26-WT-ex-del; Mus musculus; miRNA-Seq
library_construction_protocol: Small RNAs were isolated by miRNeasy kit (Quiagene). RNA samples were prepared using Illumina sample preparation kit (Illumina, San Diego, CA) according to the TruSeq Small RNA Sample Preparation Guide. The 3′ and 5′ adaptors were ligated to total RNA and reverse transcribed followed by PCR amplification. The enriched cDNA was size-fractionated and purified on a 6% polyacrylamide gel electrophoresis.  Bands containing the 18-40 nucleotide RNA fragments (140-155 nucleotides in length with both adapters) were excised as the small RNA library.