title: DNA-Sequencing of seawater from Monterey Bay, CA: 16S target markers
design: This protocol is aimed at amplifying the 16S rRNA hypervariable region 4 (16S V4) in prokaryotes. The primers (515fB and 806rB) utilized in this protocol are based on the primer utilized in Aprill et al 2015 and the Earth Microbiome Project (EMP).