title: Illumina sequencing of Human Activated Nucleosome genomic fragment library
design:            CD4+ T cells were purified from human blood as described in Barski et al., Cell, 2007. Incubating with anti-CD3 and anti-CD28 antibodies for 18 hr activated the cells. To prepare mononucleosomes, the activated T cells were treated with MNase to generate approximately 80% mononucleosomes and 20% dinucleosomes. The DNA fragments of approximately 150 bp were isolated from agarose gel, blunt-ended, ligated to the Solexa adaptors, and sequenced using the Illumina 1G Genome Analyzer as described in Barski et al., Cell, 2007.         