title: B73 HMPR Husk
design: A modified HMPR library was constructed according to Gore et al. (2009, The Plant Genome, In press). Approximately 5 ug of high molecular weight DNA was fragmented by nebulization to a size range of 300 to 500 bp. Preparation of 454 libraries, emulsion-based clonal amplification, library sequencing on the Genome Sequencer FLX System as well as signal processing and data analysis were performed as previously described by Margulies et al. (2005).