title: Genotyping by Sequencing of Avena barbata: RIL # F7.023 Double digest library.
design: Genotyping by Sequencing of double digested genomic DNA (Pst-I and Msp-I).  Well: G01 With four Barcodes: TTGCACCAG/ACATCACCG/TGTCAAT/CCGAACA