title: GSM4654215: Vegetative stage replicate 1 [NovaSeq]; Dictyostelium discoideum AX4; RNA-Seq
library_construction_protocol: Cells were dissociated into single cells from different stages by physical pipetting with PBS + 0.04% BSA. The cell numbers were counted using a microscope. Cell from each sample were subject to Chromium Single Cell Gene Expression kit V3 (10x Genomics) for library construction according to the manufacturer's instructions.