title: Paired-end MiSeq of nascent freshwater bacterial biofilm derived from planktonic cells
design: Nested PCR of 16S gene using primers from Turner, Pryer, Miao, & Palmer, 1999. Samples then sent to The Ohio State University's Molecular and Cellular Imaging Center for paired-end sequencing of the V4 region using primers 515F and 806R as described by Caporaso et al., 2012.