title: Mature oocytes diapause replicate 1
design: Mature oocytes diseccted from a single biological replicate of virgin, bloodfed Aedes albopoctus females reared under diapause-inducing conditions. RNA was extracted by homogenizing tissue in TRI Reagent (Sigma-Aldrich, St. Louis, Missouri, USA) and performing a phenol-chloroform extraction followed by an isopropanol precipitation according to the manufacturerŐs directions. Residual DNA was removed from the sample using TurboDNA Free (Ambion, Austin, Texas). The library was prepared and barcoded using the TruSeq RNA sample preparation kit (Illumina, San Diego, California, USA) at the University of Maryland Institute for Genome Science. The library was run using one-sixth of an Illumina MiSeq lane.