title: Maize transposon flanking sequence tags from the UniformMu Robertson's Mutator tagging population
design: TAIL-PCR was used to amplify random length fragments. The site specific primers annealed to the terminal inverted repeat of the Mu-element (MuTIR) as described in Settles et al (2004). A custom Amplicon A primer was synthesized for each pool with the structure 5'-Amplicon A:4bp bar code:MuTIR-3'. The custom primers were used for an annealing and extension reaction. The 24 extension reactions were pooled and ligated to Amplicon B plus biotin. The Amplicon A:MuFST:Amplicon B ligation products were then purifed following standard library construction techniques, and the products were sequenced with the Amplicon A primer.