title: Nitrogenase (nifH) amplicons from Pleurozium schreberi stems, Reivo, Sweden, senescent sections, pooled locations 1-6
design: Amplicons were generated with target-specific nifH primers IGK3_N (GCNWTHTAYGGNAARGGNGGNATHGGNAA) and DVV_N (ATNGCRAANCCNCCRCANACNACRTC), amended with Nextera XT adapter sequences on their 5' end (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG for forward primer, GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG for reverse primer). Multiplexing was carried out in a two-step PCR approach with Nextera XT indices. Pooled amplicon libraries were sequenced on the Illumina MiSeq with the paired-end 300 bp kit.