title: GSM2454144: RNA T+ KO TS13; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from approximately 20,000 cells was isolated using the RNeasy Micro kit (Qiagen) and genomic DNA was digested on column with the addition of an extra 1ul of RNase-free DNase I (Roche) to ensure complete digestion. The RNA from approximately 20,000 cellswas quantified using the Qubit RNA HS Assay and the integrity was verified using Bioanalyzer RNA Pico chips. Approximately 80-100ng of total RNA was used for the generation of strand-specific RNA-Seq libraries using the ScriptSeq v2 (Epicentre) low input libarary preparation kit according to manufacturer’s instructions. During library preparation, the purification after rRNA-depletion was performed using RNeasy Micro columns and the cDNA was purified using the MinElute PCR Purification Kit (Qiagen). The library was finally amplified with 15 PCR cycles.