title: GSM4307346: Sample_107_Incisional biopsy; Homo sapiens; miRNA-Seq
library_construction_protocol: Incisional biopsies were collected before chemotherapy from 223 patients with locally advanced breast cancer in a prospective study designed to identify the response to epirubicin (n = 109) and paclitaxel (n = 114) monotherapy. Primary response to therapy as well as long-term follow up (>10 years or death) was recorded for all patients. Tissue samples were snap-frozen in liquid nitrogen in the operating theatre and stored in liquid nitrogen until further processing. Total RNA was extracted from the biopsies using miRvanaTM kit (ThermoFisher), according to the manufacturer's instructions single sequencing were performed on Illumina HiSeq 2500 to obtain 50bp reads