title: GSM2544788: Extracellular Vesicles from 3-6mm bovine ovarian follicles; Bos indicus; RNA-Seq
library_construction_protocol: Trizol LS TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA). The libraries' average sizes were estimated using the Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA), and quantified using quantitative PCR with the KAPA Library Quantification kit (KAPA Biosystems, Foster City, CA, USA). Four lanes of a sequencing flowcell, using the TruSeq PE Cluster kit v3-cBot-HS kit (Illumina, San Diego, CA, USA), were clustered and sequenced using HiScanSQ equipment (Illumina, San Diego, CA, USA) with a TruSeq SBS Kit v3-HS (200 cycles).