title: GSM2108304: PSIΔAB male fly head rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For RNA-seq, Drosophila heads were isolated from 50mL of manually sorted and frozen males from the v16/v16 PSI mutant fly strain (PSI-null) carrying either the PSI genomic-cDNA wild-type or PSIΔAB transgenes.  Total RNA was isolated using Qiagen RNA Easy kits and polyA (+) RNA was purified using oligo (dT) Dynabeads (Life Technologies).  For iCLIP, Nuclei were isolated from suspension cultures of S2 cells by hypotonic lysis and Dounce homogenization.  Nuclei were resuspended in isotonic buffer and irradiated on ice with 150 mJ/cm2 at 254 nm in a Stratalinker 2400. NEBNext® mRNA Sample Prep kit (cat # E6110S; New England Biolabs)