title: GSM2107371: IMR5-75_DAC + EPZ6438 replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the miRNeasy Mini Kit  according to the manufacturer's protocol. Total RNA was depleted from ribosomal RNAs using the Ribo-Zero rRNA Removal Kit (epicentre) according to the manufacturer's protocol. RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer's protocol with the following changes: RNA was fragmented for 20 min at 94°C followed by first strand cDNA synthesis for 10 min at 25°C, 50 min at 42°C and 15 min at 70°C. Size selection of adapter-ligated DNA was done with a bead:DNA ratio of 0.4 (AMPure XP beads, Beckman Coulter) removing index primer and short fragments. Quality, quantity and sizing (approximately 320 bp) of the RNA Library were analyzed using a DNA High Sensitivity DNA chip run on a 2100 Bioanalyzer (Agilent Technologies). Libraries were sequenced (50 bases single-end) on the Illumina sequencing platform (German Cancer Research Center Core facility).