title: B73 UF WGS
design: Total genomic DNA was isolated from homogenized frozen 1-week-old root tissue of B73 using the DNeasy Plant Maxi Kit (QIAGEN, Valencia, CA) according to the manufacturer's protocol.  Approximately 5 ug of high molecular weight DNA was fragmented by nebulization to a size range of 300 to 500 bp.  Preparation of 454 libraries, emulsion-based clonal amplification, library sequencing on the Genome Sequencer FLX System, as well as signal processing and data analysis were performed as previously described by Margulies et al. (2005).