title: GSM2722733: dms denaturated 72hr post infection; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted using TRI ragent and phase separated using chloroform. mRNA was isolated using polyA selection kit (Ambion) Small RNA fragments were size selected via gel purification, dephosphorylated, ligated, and reverse transcribed. The resulting cDNA was circularized and PCR amplified.