title: 16S rRNA of rainbow trout gut bacteria: fed WA40 yeast
design: Bead beating, Qiagen QIAamp extraction, primers of 515F (5’-GTGCCAGCMGCCGCGGTAA-3’ and 805R (5’-GACTACHVGGGTATCTAATCC-3’) with Genejet and Ampure purification after barcoding.