title: GSM4473319: LuCaP_93_Cabozantinib_3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from flash frozen tissue from 6 xenografts of animals (3 animals treated with vehicle control, 3 animals treated with cabozantinib for 5 days) bearing LuCaP 93 tumors with RNA STAT-60 (Tel-Test).  The isolated RNA was then purified with Qiagen RNeasy Kit (Qiagen Inc.) according to the manufacturer's protocol utilizing the optional DNase treatment in solution prior to purification.  RNA concentration, purity, and integrity was assessed by NanoDrop 2000 (Thermo Fisher Scientific Inc) and 2100 Bioanalyzer (Agilent Technologies). RNA-Seq libraries were constructed from 1 ug total RNA using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit according to the manufacturer's protocol. Barcoded libraries were pooled and sequenced on an Illumina NovaSeq S1 100 flowcell generating 50 bp paired end reads.