title: Swanson_800bp_160nt
design: The shotgun library was prepared with the Hyper Kapa Library Preparation kit (Kapa Biosystems). Library insert size: 700bp - 900bp (average 750bp). The library was quantitated by qPCR, pooled with the three mate-pair libraries and sequenced on four lanes  for 161 cycles from each end of the fragments on a HiSeq2500 using a TruSeq SBS sequencing chemistry version 4. Fastq files were generated and demultiplexed with the bcl2fastq v1.8.4 Conversion Software (Illumina).