title: Fungal ITS-2 amplicons associated with Pyrola rotundifolia
design: Dual-index strategy (see Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform. Applied and Environmental Microbiology. 2013;79(17):5112-5120. doi:10.1128/AEM.01043-13.)