title: GSM3359659: MSC-Vehicle_2; Homo sapiens; RNA-Seq
library_construction_protocol: Ten wells per replicate were pooled for RNAseq. Total RNA was isolated using Qiagen RNeasy mini kit, catalog no. 74104. RNA yield and purity was obtained by NanoDrop 2000 (Thermo Fisher Scientific).  RNA Integrity was evaluated by 2100 Bioanalyzer (Agilent Technologies). RNA-seq libraries were constructed from 1 ug total RNA using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit according to the manufacturer's protocol. Barcoded libraries were pooled and sequenced on the Illumina HiSeq 2500 generating 50 bp paired end reads.