title: GSM2878216: G00253; Mus musculus; RNA-Seq
library_construction_protocol: Tumor tissue and surrounding unaffected tissue of Apc Min/+ and Apc Min/+ ::ADAM17 ex/ex mice were prepared freshly. To isolate total RNA from animal tissue for RNA sequencing, the RNeasy Mini Kit from Qiagen (Hilden, Germany) was used according to supplier's instructions including a QIAshredder column from Qiagen. Individual strand-specific sequencing libraries were prepared with the TruSeq stranded mRNA kit (Illumina) from 500 ng total RNA.