title: Pacbio RSII subreads of Durian Monthong variety
design: Young leaf tissue was collected from a single plant and used for DNA extraction with the standard CTAB method followed by clean-up using a DNeasy Mini spin column from Qiagen.  DNA was used to prepare libraries for the PacBio RSII following the Pacific Biosciences 'Procedure and Checklist - 20 kb Template Preparation Using BluePippin Size-Selection System' protocol. DNA (10 ug) was sheared with a Covaris gTube, 4500 rpm for 2 minutes and the BluePippin cassette used was '0.75%DF Marker S1 high-pass 15-20kb' with selection of 12-50 kb. Sequencing was performed for 8 cells on the PacBio RSII.