title: 16S rRNA amplicon sequencing to characterize the pelagic microbial community in coastal Monterey Bay
design: The 16S rRNA gene amplicons were prepared from each sample and sequenced using the MiSeq platform (Illumina; iTag amplicon sequencing), as part of a Community Science Program (CSP) project with the DOE Joint Genome Institute (JGI). Universal (Bacteria and Archaea) V4-V5 primers 515F-Y (5_- GTGCCAGCMGCCGCGGTAA) and 926R (5_- CCGYCAATTYMTTTRAGTTT) (39) were used for amplification.