title: RNAseq of C.thermocellum: sugarcane bagasse as carbon source
design: Total RNA extraction was carried using TRizol Reagent (Invitrogen), according to the manufacturers instructions with an additional step of bacterial cell wall enzymatic hydrolysis. This step consists to  resuspend the cell pellets with TE buffer containing 10 mg/mL lysozyme and incubates for 1 hour at 37 C. Extracted RNA was further submitted to a purification step using RiboPureTM bacteria RNA purification kit (Ambion, Life technologies, Inc.USA), according to the manufacters instructions. RNA quality was assessed by electrophoreseis on agarose gels 1% (m/v), integrity and quantification were assessed using analysis on Agilent Bioanalyzer 2100 (Agilent Technologies). RNA samples were sent to Eurofins Genomics Inc (USA), which carried library preparation and sequencing