title: DNA-Seq of soil
design: 40 pooled samples (first 5cm of mineral soil) from 28 m radius circular plot; 15 ml DNA extracted using MoBio Soil Maxi kit; PCR targeting the V7 region (~110 bases) of the 18S rRNA gene using the forward and reverse primers (5'-TTTGTCTGSTTAATTSCG-3') and (5'-TCACAGACCTGTTATTGC-3') of eukaryote; Macrogen Companie amplification and Illumina MiSeq sequencing.