title: RNA-Seq wheat rhizosphere: suppressive soil for Rhizoctonia solani AG8
design: RNA was extracted from the rhizosphere of 8 week old wheat plants                 growing in a field soil which was suppressive to the plant pathogen Rhizoctonia                 solani AG8 using a RNA PowerSoil Total RNA Isolation Kit (Mo-Bio). Ribosomal                 subtraction was performed using a Ribo-Zero Magnetic kit for Bacteria (Epicentre,                 Madison, WI, USA). Double-stranded cDNA was generated from the mRNA enriched RNA                 using Superscript II reverse transcriptase (Invitrogen, Carslbad, CA, USA). Library                 preparation using a TruSeq Stranded mRNA kit, processing and sequencing were                 performed in-house using the Illumina HiSeq 2000 with paired-end (PE) 100 bp                 sequencing of templates ~295 bp long. Index sequence                 TCTCGCGC-AGGCGAAG