title: IsoSeq_mixed stage transcript analysis for Dermanyssus gallinae
design: Full-length transcripts were obtained from total RNA from mixed stages of D. gallinae, analysed with PacBio Iso-Seq pipelines within the PacBio SMRT Portal v5.0.1.10424 (Minimum Quiver/Arrow Accuracy=0.85; Min GMAP Alignment Identity=0.8), generating 13,612 high-quality and 53,082 low-quality isoforms post-Quiver polishing (mean read length 1,142bp)