title: GSM3133258: toluene_15min_rnaseq 2; Sphingobium chlorophenolicum L-1; RNA-Seq
library_construction_protocol: Total RNA was purified using Ambion Purelink RNA mini kit according to manufacturer's instructions. Cells were homogenized using Qiagen Qiashredder columns. RNA was eluted in Rnase-free water. After elution, samples were treated with Turbo DNA-free kit (Ambion). RNAtag-seq libraries were prepared as described in (Shishkin, Alexander A., et al. "Simultaneous generation of many RNA-seq libraries in a single reaction." Nature methods 12.4 (2015): 323.).