title: RNA-Seq of Macrobrachium tolmerum forward and reverse : 0ppt
design: cDNA libraries were prepared following TrueSeqV1 Standard mRNA Sample Prep kit (Illumina, USA) instructions using 4 g of total RNA. cDNA libraries were prepared according to the methods including:  i) poly(A) tail mRNA capture and fragmentation, ii) cDNA synthesis from size selected fragments, iii) subsequent purification of cDNA fragments, iv) repair and adenylation of 3 ends, v) bar coding of each cDNA library and vi) enrichment of cDNA libraries via 15 cycles of PCR amplification. The quality of each cDNA library was assessed using Bioanalyzer (Agilent 2100, USA); quantity was estimated using Qubit2.0 Fluorometer (Invitrogen, Life Technologies) and RT-qPCR (BJS Biotechnologies, UK). cDNA libraries were used for sequencing on a NextSeqTM 500 Sequencer.