title: GSM3302730: PGM-111: DE442_TEXplus_5prime_dRNA-seq; Rhodobacter capsulatus DE442; RNA-Seq
library_construction_protocol: 500μ of culture were transferred into 100μl 95% Ethanol and 5% Phenol. Samples were spun down for 2 minutes and the pellets were stored at -80°C. Small RNA was extracted following the Macherey-Nagel protocol for DNA, RNA, and protein purification kit for small RNA fraction. Libraries for 5' and 3' targeted sequencing were prepared following a newly developed protocol based on dRNA-seq. Libraries for total RNA seq were prepared following the Ion Torrent Ion Total RNA-Seq Kit V2 for whole RNA Libraries protocol.