title: RNA-Seq of Tagetes erecta: mature leaves
design: Leaf or flower samples, were grounded to fine powder. According to the manufacturers  protocols, total RNAs were isolated using the Trizol reagent followed by treatment with RNase-free DNase I. The quality of RNAs was checked using Agilent 2100 Bioanalyzer. RNA-Seq libraries were prepared and sequenced on the HiSeq 2000 system, following the manufacturer s instructions.