title: Mycoplasma hominis 132 complete sequence
design: gDNA extraction + The genome Mycoplasma hominis 132 was sequenced as follow: mate pair with the Illumina MiSeq system (775X average coverage). Paired-end reads were also generated with Illumina GAIIx technology (1700X average coverage). Sequencing resulted in approximately approximately 26 million mate pair reads on average, with lengths ranging from 75 to 251 bp and insert sizes of 5000 and 2000 bp. Reads were filtered using NGS QC toolkit1  (version 2.2.3), with 20 cutoff quality score and 70% cut-off read length for high quality reads. . In the assembly step, two state of the art, de novo assembly tools were used on each dataset: ABySS2  (version 1.3.5) and CLC assembly cell3  (version 4.2.0). To make the most of the complementarity of these tools, the two assemblies obtained for each dataset were merged using two softwares, Mix4  and GAA5  (version 1.1). Finally, based on the paired information, the contigs that were obtained in the previous step were organized in scaffolds with Opera 6  (version 1.3.1) scaffolding tool.