title: Genotyping by Sequencing of Avena barbata: RIL # F7.136 Double digest library.
design: Genotyping by Sequencing of double digested genomic DNA (Pst-I and Msp-I).  Well: D05 With four Barcodes: TGTGACAAGA/ATGGTGA/CTCGCGG/TCGCG