title: DNA-Seq of litter
design: 40 pooled samples (all litter depth) from 28 m radius circular plot; 15 ml DNA extracted using MoBio Soil Maxi kit; PCR targeting the V3-V4 region (~460 bases) of the 16S rRNA gene using the forward primer (5'-CCTACGGGNGGCWGCAG-3') and reverse primer (5'-GACTACHVGGGTATCTAATCC-3') of prokaryotes; Macrogen Companie amplification and Illumina MiSeq sequencing