title: 18S rDNA amplicons - 9 barcode groups, 12 locational/sieve-size fraction samples per barcode
design: DNA was extracted from 108 samples derived from 3 reference locations (Boronia Park, Tambourine Bay and Woodforde Bay) and 3 sieve-size fractions (macro >500um, meio 500-40um and micro <40um). Conserved 18S primers were used to amplify a 200-300bp variable part in the 3'region of the eukaryotic 18S rRNA gene. 9 fusion primers with A and B adapters & 10 base barcodes were added by PCR to enable tracking of 18S rDNA sequences back to 9 groups of 12 samples derived from the 3 reference locations and 3 sieve-size fractions. Primer A was used to sequence through barcodes.