title: GSM3463021: Rad21WT primary dissociated cortical neuron Rep1; Mus musculus; RNA-Seq
library_construction_protocol: For polysome immunoprecipitation, homogenates from DIV10 dissociated cortical neurons from Rad21lox/lox;NexCre/+;RiboTag+ and Rad21+/+;NexCre/+;RiboTag+ were prepared as described in (Sanz et al., 2009) with minor modifications. RNA was extracted with QIAshredder and RNeasy mini kit (Qiagen). RNA-seq libraries were prepared from 300 ng of total RNA (RIN>9.0) with TruSeq Stranded Total RNA Human/Mouse/Rat kit (Illumina).