title: GSM4476810: HT29 cells, Shp53_2; Homo sapiens; ncRNA-Seq
library_construction_protocol: exoRNeasy Midi and Maxi Kit (Qiagen, Hilden, Germany; Cat. # 77144 After sample QC, library was constructed by Small RNA Sample Pre Kit. The final cDNA library was ready after a round of sequencing adaptor ligation, reverse transcription, PCR enrichment, purification and size selection (18-40 nt).