design: Grosmannia clavigera spores from strain kw1407 were spread onto cellophane overlaid on 1.5% agar containing 1% malt extract in 15 cm petri dishes. The fungal spores were incubated at 22 degrees Celsius in the dark for eight days, and the mycelia were removed from the cellophane and pooled.  DNA was extracted from mycelia as previously described (Moller EM et al 1992) but without first lyophilizing the mycelia.  For preparing the ~200-bp library on the Illumina GAii sequencer, 5ug of DNA was sonicated for 10 minutes, alternating one minute on and off, using a Sonic Dismembrator 550 (Fisher Scientific, Ottawa, CAN). Sonicated DNA was then separated in an 8% PAGE. The library was constructed from the eluted 190-to-210-bp fraction of DNA using Illumina's genomic DNA kit, following their protocol (Illumina, San Diego, CA). Four lanes in a single flow-cell were sequenced to 42 cycles using v.1 sequencing and cleavage reagents. Data was processed using Illumina's GA pipeline (v.0.3.0 beta3).