title: 18S sequence of Xyleborus affinis:Gallery:30 days of culture
design: Sequencing libraries were constructed using Nextera XT adapters (Illumina Inc.) using Kappa polymerase (Kappa HiFi Hotstart Ready Mix, cat. No. KK2602, Kappa Biosystems), purified using Agencourt Ampure XP magnetic beads (BeckmanCoulter, cat. No. A63881) and pooled to an equimolar concentration to be sequenced in a MiSeq run using a MiSeq Reagent kit V3 (600 cycle).