title: NextSeq 500 sequencing; A PP2A-Pab1-mediated crosstalk between TORC1 & TORC2 regulates the differentiation response in fission yeast
design: A PP2A-Pab1-mediated crosstalk between TORC1 & TORC2 regulates the differentiation response in fission yeast
library_construction_protocol: Cells were grown at temperature of 25C EMM (Moreno et al. Methods Enzymology 194: 795) containing NH4Cl as a nitrogen source. Total RNA preparation was performed with MasterPureTM Yeast RNA Purification Kit (Epicentre) following the manufacturers instructions. Libraries were generated using TruSeq Stranded mRNA Library Prep Kit according to the manufacturers instructions