title: Ion Torrent PGM 100bp Whole Gneome Shotgun Sequencing of E.coli Sakai
design: The 100bp sequencing of the Sakai strain were done with Bioruptor shearing, 316 chip, and TSS v1.5.2. Quality control analyses for the library were performed with a Caliper Labchip GX using the DNA High Sensitivity Assay (Caliper Life Sciences, Mainz, Germany). Library concentrations were determined with the Qubit dsDNA HS Assay Kit (Life Technologies).