title: Genotyping by Sequencing of Avena barbata: RIL # F7.112 Double digest library.
design: Genotyping by Sequencing of double digested genomic DNA (Pst-I and Msp-I).  Well: D04 With four Barcodes: GACGGCA/GCTCGCCAT/GGAACGA/CGTTGGT