title: GSM2977233: Embryo-Isoseq; Xenopus tropicalis; RNA-Seq
library_construction_protocol: Iso-seq libraries were constructed by the Genomics Core Laboratory, Washington State University. The Iso-seq method includes several major steps: poly(A)+ RNA enrichment, full length cDNA synthesis,  PCR amplification, size selection. Two fractions of cDNA, 1-2kb, 2-3kb were collected and sequenced. Total RNA was harvested using Trizol reagent.