title: 454 sequencing of Saccharomyces cerevisiae H3 nucleosome sample #2 genomic fragment library
design: H3 or H4 TAP-tagged BY4741 cells were formaldehyde-crosslinked, lysed with beads/vortexing, and digested with micrococcal nuclease.  Solubilized, nucleosomal chromatin was immunopurified for the histone of interest using IgG Sepharose (that recognizes the TAP tag), and mononucleosomal DNA was gel-purified.  DNA was then prepared for sequencing using manufacturer's recommended protocol.