title: 16S rRNA V4 library of feshwater metagenome: Unfiltered, no substrate, sampled after 333 hours, biological replicate 2
design: One-step PCR dual-indexing primer system decribed by Fadrosh et al., 2014. rRNA-annealing parts of the primers corresponded to standard F515-R806 primers with slight modifications: F515 GTGBCAGCMGCCGCGGTAA; R806 GGACTACHVGGGTWTCTAAT