title: RNA from thoc5[e00906]/thoc5[1]
design: Ribosomal rRNA depleted (hybridize to antisen rRNA oligo and digested with thermostable RNase H) RNA samples were fragmented, reverse transcribed, end-repaired, A-tailed, ligated with adapters and PCR amplified to generate library. The strand specificity is achieved by dUTP incorporation and UDG digestion.