title: GSM2719819: prdm14_puro_esc3; Mus musculus; OTHER
library_construction_protocol: gDNA was extracted using the QuiAmp® DNA Micro. The gDNA was digested with DpnI, adaptors were ligated and then digested with DpnII and pcr amplified. DNA was sonicated to an average size of 300bp using a Diagenode Bioruptor and DamID adaptors removed with AlwI digestion.  Sonicated material was end-repaired, A-tailed, sequencing adaptors ligated and PCR amplified.