title: Brewery Waste MEC, Day 91-supernatant
design: Active microbial population within supernatant of brewery waste MEC. Sample was collected on day 91, and RNA was extracted from cells. RNA was randomly reverse transcribed, then PCR was performed with A109f-U529r primers (Archaeal, V2-V3) and with B27f-U529r (Bacterial, V1-V3) to create 16S rRNA amplicons. Barcode was added to 5' end of the reverse primer: ACGAGTGCGT. These were sequenced with PacBio, and only circular consensus reads were used. Data submitted contains the raw read and the quality score from all reads making it through our preprocessing pipeline that REMOVED all reads containing: any ambiguous base (quality score less than 1), greater than 8 homopolymers, less than 300 bp, greater than 700 bp, regions of low quality score (average less than 25 over rolling window of 10 bp), primer or barcode mismatch greater than 1, chimeras. Note: Data used in our analyses involved taking these reads, aligning them to core greengenes sequences, and trimming off primers as well as parsing the bacteria from archaea.