title: PICO-LOVE offshrore transects
design: We extracted DNA using the Gentra Puregene Yeast/Bact. Kit (Qiagen) according to the manufacturers instructions supplemented with one round of bead beating (60 seconds).  The fungal internal transcribed spacer (ITS) region was amplified using the dual index PCR primers ITS1-F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') (Bruns & Gardes, 1993).