title: Illumina sequencing of RNA Pol2 ChIP in A549 Cells after 1h, 0.02% v/v Ethanol Treatment (Replicate 1)
design: A549 cells were treated for 1h with either 100nM Dexamethasone, or an equal volume of ethanol as a vehicle control, as indicated.  Chromatin was formaldehyde crosslinked, sheared, and immunoprecipitated with anti-Pol2 antibody. Purified DNA was made into Illumina sequencing libraries by addition of Illumina linkers to repaired ends, PCR amplification, gel extration of fragments ~150-250bp (excluding linkers), and a second PCR amplification.  Purified DNA was used for cluster generation and sequencing using the Illumina 1G Genome Analyzer.