title: GSM2977232: Adult-femalepool-HATTseq; Xenopus tropicalis; RNA-Seq
library_construction_protocol: HATT-seq library was constructed based on our protocols. The HATT-seq method includes several major steps: poly(A)+ RNA enrichment, 1st strand cDNA synthesis, fragmentation,circularization,size selection and PCR amplification Total RNA was harvested using Trizol reagent.