title: Shandong well watered, leaves, Eutrema salsugineum
design: E. salsugineum reads were sequenced using Illumina technology from Shandong ecotype rosette leaves grown under control, unstressed conditions as outlined in MacLeod et al. (2015). Fully-expanded leaves were used for RNA sequencing, and were collected between 8 and 10 hours into the day cycle. RNA was extracted from leaves flash-frozen using liquid nitrogen using a modified hot borate method as described Champigny et al. (2013). A quality control analysis was competed on the RNA using RNA Nano 600 chips on a Bioanalyzer 2100 and purified using three on-column purifications by Genelute mRNA miniprep kit (Cat. No. MRN10, Sigma). Finally, preparation of cDNA for sequencing was performed with the NEBNext multiplex cDNA synthesis kit for Illumina using random hexamers (Cat. No. E7335, New England Biolabs, Ipswich, MA). Cleanup of fragmented RNA was performed with Agencourt AMPure XP Beads (Cat. No. 163987, Beckman Coulter, Mississauga, ON) following the manufacturer's protocol.