title: WXS for Mus musculus 4 months  female thyroid induced by tamoxifen
design: Samples were collected from 5  thyroid tumors and 5 kidneys. Genomic DNA extraction was performed with the  All prep DNA/RNA kit (Qiagen, Hilden, Germany). Genomic DNA was sheared via  sonication using a S220 focused-ultrasonicator (Covaris, Woburn, MA). DNA  libraries were constructed using the SureSelectXT Mouse All Exon kit (Agilent  Technologies, Santa Clara, CA), according to the manufacturers  recommendations. The constructed libraries were sequenced using Illuminas  High Output Kit (150 cycles) on a NextSeq500 (Illumina, San Diego, USA) with  an average depth of 140x for tumor and 70x for normal (kidney) samples.