title: GSM4040233: RNA-seq_WT-2d-Rep2; Mus musculus; RNA-Seq
library_construction_protocol: 3T3-L1 cells were collected at the indicated time points and total RNA was isolated using the RNeasy kit (Qiagen) according to the manufacturer's instructions. The total RNA was then enriched for polyA+ RNA using Dynabeads Oligo(dT)25 (Invitrogen).  The polyA+ RNA was then used to generate strand-specific RNA-seq libraries as described previously.  The RNA-seq libraries were subjected to QC analyses (i.e., number of PCR cycles required to amplify each library, the final library yield, and the size distribution of final library DNA fragments) and sequenced using an Illumina HiSeq 2000