title: RNASeq of Ca. Electronema sp. GS metagenome: Oxygen reduction - Replicate 1
design: Freshwater cores with Ca. Electronema sp. GS were grown under oxic conditions. Total RNA from each sediment sample was extracted using the RNeasy PowerSoil Total RNA Kit (Qiagen). The RNA was concentrated with the kit RNA Clean & Concentrator (Zymo Research, USA) with an in-column DNase treatment (Ambion, USA) and stored at -80 C before sequencing. Illumina sequencing (HiSeq2500, SR50 reads) was performed by DNAsense (Aalborg, Denmark), after 16S and 23S rRNA removal with the Ribo-Zero rRNA Removal Kit for Bacteria (Illumina, USA).