title: GSM2719286: 12h_4; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI)