title: DNA sequencing of circular virome of Ambystoma mexicanum tissue
design: 1) mincing of tissue 2) lysis of cells via detergent 3) nuclease digestion 4) particle enrichment via ultracentrifugation over optiprep gradient 5) collection/fractionation of optiprep gradient 6) rolling circle amplification (aka MDA) of each fraction 7) DNA purification 8) library prep: Nextera XT 9) Illumina sequencing