title: GSM3131877: PD014_BCF2R5_RibosomeProfiling; Mus musculus; OTHER
library_construction_protocol: Ribosome Profiling: Monosomes were isolated from RNase I-digested brain lysates on a sucrose gradient fractionator. RNA-Seq: Total RNA was isolated from brain lysates using a Qiagen RNeasy kit (cat no. 74104) and ribosomal RNA was depleted using the Ribo-Zero rRNA removal kit from Illumina (Cat no. MRZH11124) according to the manufacturer's instructions. Ligation-free ribosome profiling : Ligation-free ribosome profiling libraries were prepared from dephosphorylated foot-prints (~ 28-34 nucleotides in length) using a commercially available kit (SMARTer small RNA-Seq Library Preparation Kit, Clontech, Cat no. 635029) following manufacturer's instructions.   RNA-Seq library construction : rRNA depleted total RNA samples were converted to a strand-specific sequencing library using the NEBNext® Ultra™ Directional RNA Library Prep Kit from Illumina (Cat no.E7420S).