title: GSM4476720: A549 cells, Module 3 Time End Untreated replicate 3; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted using phenol:chloroform followed by ethanol precipitation. sgRNAs were amplified by PCR using primers specific for the regions of the plasmid flanking the sgRNA (FwdU6-1: 5'-CAAGGCTGTTAGAGAGATAATTGG-3' R2: 5'-CGACAACAACGCAGAAATTTTGAAT-3'). The PCR product was sequenced with 80–100 million single-end 50 base pair reads. Crispr Knock-out screen