title: GSM4308841: Bru-seq_siPUM_rep4; Homo sapiens; OTHER
library_construction_protocol: Bru-seq and BruChase-seq were conducted as described in Paulsen et al. (doi: 10.1016/j.ymeth.2013.08.015) in HEK293 cells grown in the presence of siPUM1/2 or siNTC. SEQRS was conducted on PUM1 PUM-HD and PUM2 PUM-HD as described in Campbell et al. (doi: 10.1038/nsmb.2847) with minor modifications including the use of Magnetic Halolink beads.