title: RADseq of Carcinus maenas: muscle
design: 1 µg DNA, Sbf1 restriction enzyme, sheared with sonicator, individual barcodes appended, 22 individuals per sampling location pooled and sequenced on 1 lane, PCR cycles 98 °C for 30 seconds; 14 cycles of 98 °C for 30 seconds, 65 °C for 30 seconds, 72 °C for 30 seconds; 1 cycle of 72 °C for 5 minutes