title: Phaeoacremonium minimum isolate Pm1119 Genome sequencing (SMRT DNA sequencing)
design: Around 15 mg of high-quality genomic DNA was sheared using a Megaruptor (Diagenode, Denville, NJ, USA). After shearing, fragmented DNA was processed using a SMRTbell Template Prep Kit 1.0 (Pacific Biosciences, Menlo Park, CA, USA). Libraries were size selected at 12–50-kb cut-offs using a Blue Pippin (Sage Science, Beverly, MA, USA), submitted to a final DNA repair treatment (SMRTbell Damage Repair Kit, Pacific Biosciences) and then sequenced using a PacBio RS II platform (DNA Technologies Core Facility, University of California, Davis, CA, USA).