title: 454 sequencing of cDNA from C. spinosa leaf
design: Total RNA was isolated from leaves of 28 day old plants.  mRNA was selected by two rounds of oligo(dT) purification.  cDNA was synthesized using the Clontech SMART cDNA Libary Construction kit.  dscDNA was prepared for pyrosequencing using the standard 454 shotgun library preparation kit.