title: GSM2264867: GM12878 spiked with Sequins Mix B rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from K562 and GM12878 cells using TRIzol (Invitrogen). DNase treatment was performed with TURBO DNase (Life Technologies) followed by a cleanup with RNA Clean and Concentrator-25 Kit (Zymo Research). K562 total RNA was spiked with RNA sequins Mix A prior to library preparation. GM12878 total RNA was spiked with RNA sequins Mix B prior to library preparation. The neat samples of RNA sequins were sequenced without addition of any natural RNA. RNA was ribo-depleted using Ribo-Zero™ Magnetic Kit (Human/Mouse/Rat) (Epicentre), and libraries were prepared using KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems).