title: GSM2453817: NF2/Chr22_miRNA-seq [MN-64846]; Homo sapiens; miRNA-Seq
library_construction_protocol: 10 to 15 frozen sections of each tumor block or dura sample were collected for each experiment. miRNA was extracted using the mirVANA miRNA isolation kit (Ambion, AM1560). Samples were subjected to standard Illumina  library construction after assessment for material quality and quantity. Sequencing was performed on Illumina HiSeq instruments using 75 basepair, single-end reads and multiplexing 8 tumor samples per lane