title: GSM3418013: Islet1Cre;Ebf1fl/- 1; Mus musculus; RNA-Seq
library_construction_protocol: Embryos for each genotype were obtained by crossing either Ebf1+/-;Dlx5/6::Cre or Ebf1+/-;Islet1Cre/+ males with Ebf1fl/fl females. Embryonic tissue from individual dissected embryos was collected in RNAse free-conditions and stored in RNAlater solution. The genotype of each embryo was attested by PCR. Libraries were prepared according to Illumina's instructions accompanying the TruSeq Stranded mRNA  Kit (Part# 20020594), with 400ng of total RNA