title: GSM4654608: Retina WT 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA quality was verified by the University of Pittsburgh Health Genomics Core using Agilent   Tapestation RNA and Qubit Fluorometric Quantification. RNA samples with a   RNA integrity number of (RIN) >7 were utilized. Briefly, cDNA was synthesized with   SuperScript IV and strands underwent Adenylation of 3' ends followed by adapter   ligation and library amplification with indexing. Library preparation was conducted with  TruSeq Total RNA library kit (Illumina) using at least 1 µg of total RNA following the manufacture's  instruction. Modifications during the library preparation include rRNA reduced RNA cleaned up   was done with AMPureXP beads and library amplified with 13 cycles of PCR and cleaned up with   37µl AMPureXP beads. Sequencing was performed using 1 High Output 150 flowcell. Library was prepared following manufacturer's instructions with the following modifications. RNA Seq, Library kit:TruSEq Total RNA, Library type: Stranded Total RNA