title: 454 sequencing of Hydra magnipapillata genomic fragment library
design:                    For isolation of DNA for genome sequencing, the 105 strain was re-cloned from a                    single polyp in the Bode lab at UC Irvine using standard culture conditions.                    About 1000 polyps were harvested from the resulting culture and used to                    prepare DNA for genome sequencing. A single fragment library (400-800 bp)                    was prepared for 454 sequencing using standard procedures of nebulization,                    size-selection, adapter-ligation, emulsion PCR and pyrosequencing to approximately                   1.5X coverage of the genome.                  