title: Illumina sequencing of A549 mRNA after 1h, 0.02% Ethanol Treatment (Replicate 1)
design: A549 cells were treated for 1h with either 100nM Dexamethasone, or an equal volume of ethanol as a vehicle control, as indicated.  Total RNA was extracted, followed by two rounds of poly-A selection to select fo messenger RNA (mRNA) was fragmented, reverse-transcribed into cDNA using random primers, followed by second strand DNA synthesis. Resulting double-stranded DNA was made into Illumina sequencing libraries by addition of Illumina linkers to repaired ends, gel extration of fragments ~175-225bp (excluding linkers), and 15 cycles of PCR amplification.  Purified DNA was used for cluster generation and sequencing using the Illumina 1G Genome Analyzer.