title: 16s rRNA target sequencing from soil microcosm  under fipronil F2
design: For metagenomic library construction, primers for V4 and V5 regions of the 16S ribosomal RNA gene were used for PCR amplification (515F 5'-TGTGNCAGCMGCCGCGGTAA-3' and 926R 5'-barcode-CCCCGYCAATTYMTT-3') (Quince et al. 2011)