title: GSM4658057: S01_EX_7816_healthy: Healthy donor #1; Homo sapiens; RNA-Seq
library_construction_protocol: Total extracellular vesicle RNAs are isolated from plasma using exoRNeasy Serum/Plasma Maxi kits (Cat# 77164, QIAGEN) RNA libraries were prepared for sequencing using Ovation RNA-Seq System V2 (NuGEN) kit following the manufacturer's instructions. The amplified cDNA was fragmented using a Bioruptor (Diagenode), adapters were ligated to cDNA using TruSeq ChIP Sample Preparation kit (Illumina, San Diego, CA, USA), the DNA fragments were size-selected (300–350 bp) after electrophoresis on a 2.5% agarose gel, and the selected DNA was subjected to 17 cycles of PCR amplification.  Library quality was determined using a Bioanalyzer 2100 instrument and the final libraries were sequenced using the Illumina HiSeq 2500 instrument.