title: NextSeq 500 paired end sequencing; Transcriptomic response of Dickeya dadantii to DNA relaxation by novobiocin
design: Transcriptomic response of Dickeya dadantii to DNA relaxation by novobiocin
library_construction_protocol: Cells were collected in exponential phase (OD=0.2 at 600nm) or transition to stationary phase (OD=1.5 at 600nm).  Growth in minimal medium M63 supplemented with sucrose (0.2% wt/vol)  Treated samples were subjected to 15 min novobiocin shock (100 ug/ml) Total RNA from each D. dadantii sample was isolated using the frozen acid phenol method (Maes et Messens 1992), and submitted to Vertis Biotechnologie AG for ribosomal depletion (Illumina RiboZero kit) and RNA fragmentation. Strand-specific cDNA libraries were constructed by Vertis Biotechnologie AG.