title: GSM4156677: leukemic iMLL-AF9+ Lin- ckitlow cells from mice with nestin+ cell depletion (III); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Arcturus Picopure RNA Isolation Kit (Thermo Fisher Scientific). The RNA sequencing library was prepared with the TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA) to construct index-tagged cDNA. RNA was amplified and prepared for RNA-Seq using the Ovation RNA-Seq System v2 (NuGEN).