title: GSM2304577: WT-vein-2; Cucumis sativus; RNA-Seq
library_construction_protocol: Leaf veins and fruits four days before anthesis of WT and transgenic cucumber plants were collected for RNA-seq analysis. Total RNAs were extracted using the RNA extraction kit (Huayueyang, Beijing, China), and RNA quality check were performed as previously described (Sun et al., 2016). Two biological replicates were performed for each tissue sample. RNA-seq library construction was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA) according to the manufacturer’s instructions and four index codes were added to attribute sequences to different samples(Wang et al., 2009). RNA libraries were prepared for sequencing using standard Illumina protocols