title: Bacterial composition of the artisanal Mezcal of Guerrero WIF1b
design: For the nested PCR, the 16S rRNA gene was first amplified  using primers 27F and 1495R. Second PCR was carried out using Forward primer that contains an A adapter for massive sequencing, an Ion Xpress barcode and Reverse primer containing Truncated P1 (TrP1) adapters.