title: 16S iTag Stordalen Mire
design: Sequenced using V4 region with 515F/806R primers. The conditions for PCR were: 94C for 3 min to 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; with a final extension at 72 C for 10 min.