title: GSM2720232: AR-52116_M5CRe-; Mus musculus; RNA-Seq
library_construction_protocol: CD45+ Ly6G– CD11b+ F4/80+ cells were sorted into cold PBS containing 5% BSA and were sedimented by centrifugation. An aliquot of the sorted cells was subjected to reanalysis to determine the degree of sorting purity. Cells were resuspended in 350 μl of RLT+b-ME buffer (RNeasy Micro Kit, Qiagen), stored at -80°C and used for Illumina-based sequencing. RNA quantity and quality was determined by measurement on the Qubit device as well as using the Agilent BioAnalyzer 2100. Only the samples with the RNA integrity number above 8 were sequenced. Samples were prepped accordingly to the workflow for strand specific RNA-Seq library preparation (Ultra Directional RNA Library Prep, NEB)