title: GSM3729215: Rapa_1; Homo sapiens; RNA-Seq
library_construction_protocol: HeLa cells were collected and resuspended with PBS into a 1.5-ml microfuge tube after treatments. Samples were centrifuged at 1000 rpm for 3 mins to pellet the cells at room temperature. Total RNAs were extracted with the RNeasy Mini kit (QIAGEN, Cat. No. 74104). RNA libraries were prepared for sequencing using standard Illumina protocols