title: RNA-Seq of Callitropsis nootkatensis: First year seeds
design: RNA was extracted using PureLink Plant RNA Reagent (Ambion) and further purified by RNeasy Plant Mini (Qiagen).  RNA quality and quantity were assessed by NanoDrop spectrophotometer and the Bioanalyzer RNA 6000 Nano Kit (Agilent).  Sequencing was completed at the McGill University and Gnome Qubec Innovation Centre, Montral, Canada.  rRNA-depleted libraries (Plant Leaf Ribo-Zero, Illumina) were prepared using the KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems) and were paired-end sequenced on an Illumina HiSeq 2500 with 125 bp reads, using multiplexing to sequence four libraries per lane.