title: AB SOLiD sequencing of Human HapMap individual NA18507 genomic paired-end library
design: We prepared four libraries by shearing 200µg of NA18507 genomic DNA to 1.5kb.  Libraries were prepared as a single sample until circularization, and then the sample was split into two parts to compare library excision when using S1 nuclease alone or a combination of T7 exonuclease and S1 nuclease.  Prior to amplification the two libraries were once again divided in half to compare amplification efficiencies and bias between Invitrogen's Platinum Supermix and Applied Biosystem's SOLiD Library Amplification Master Mix.  The slides correspond to the library prep as follows:  CLARA_20080903_1 S1-Supermix,  JOAN_20080829_1 S1-Master Mix, CLARA_20080903_2 T7/S1-Supermix,  JOAN_20080911_2 T7/S1-Master Mix.