title: GSM3417504: Lcell_replicate_T3Dneg_DARTseq_0; Mus musculus; RNA-Seq
library_construction_protocol: The following steps are identical to those performed using standard Drop-seq protocol (Macasko, 2015). Briefly, droplets containing beads and lysed cells were broken and washed. Complementary DNA was created using Maxima Reverse Transcriptase (Thermo Fisher) and a transcript switch oligomer. cDNA was amplified using 15 cycles of PCR. Amplified cDNA libraried were tagmented and amplified using Nextera. Illumina sequencing was performed on the libaries using 20x130 paired-end sequencing.