title: GSM3066256: Culture 3; Spiroplasma poulsonii; RNA-Seq
library_construction_protocol: RNA was extracted from (i) the hemolymph of 30 1-week-old infected flies and (ii) 20 ml of 4-month-old in vitro culture pelleted by 30 min of centrifugation at 16,000 X g by the TRIzol method following the manufacturer's instructions. Three independent replicates were prepared for each condition. RNA quality was assessed on a Fragment Analyzer (Advanced Analytical Technologies, Inc., Ankeny, IA, USA. RNA-seq libraries were prepared using 25 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) without the polyA selection step.