title: ChIP-seq of cardiac and embryonic stem cells
design: ChIP-seq was performed from FACS-sorted cardiac myocyte nuclei (Gilsbach et al., Nat. Commun. 2014). For immunoprecipitation (used antibody: Diagenode, C15410193), the ChIP-IT High Sensitivity Kit (Active Motif) was used with 200 ng of chromatin according to manufacturer's instructions. Sequencing libraries were prepared from the resulting DNA with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). To avoid over-amplification of the sequencing library, test qPCR-amplification was carried out to determine minimum cycles for final PCR.