title: GSM2722594: Chicken_heart_M_rep3; Gallus gallus; miRNA-Seq
library_construction_protocol: Total RNA was extracted from 10 to 20 mg of flash frozen tissue using the Qiagen RNeasy Micro Kit with lysis buffer RLT/40mM DTT and RWT wash buffer,  and the Qiagen miRNeasy Mini Kit according to the manufacturer’s instructions. Small RNA fraction was purified from 0.5 – 2 μg total RNA by denaturing polyacrylamide gel electrophoresis. Small RNA libraries were prepared for sequencing using the NEXTflex Small RNA-Seq Kit v2 (NF) according to the manufacturer’s instructions. For two of the samples, libraries were also prepared with the lllumina TruSeq Small RNA Library Prep Kit (IL) according to Illumina's instructions and the HD protocol (HD) developed by Sorefan et al, 2012 (DOI: 10.1186/1758-907X-3-4). Libraries were sequenced on an Illumina HiSeq 2500 System.