title: RAD-seq of Oncorhynchus mykiss: juvenile finclips
design: Genomic DNA extracted from finclips using Quant-iT PicoGreen dsDNA Assay kits. gDNA was digested using the restriction enzyme pst1 and and individually barcoded using a six-nucleotide barcode adapter sequence.  Barcoded individuals were then divided and evenly pooled into two separate RAD libraries. Libraries were mechanically sheared to generate DNA fragments with length between 200-700 bp using a Bioruptor 300 sonicator (Diagenode, Denville, NJ, USA). Fragments were size-selected and isolated using an Agencourt AMPure XP bead purification system