title: Phomopsis longicolla sequence at Purdue Genomics
design: 1 ug of genomic DNA was subjected to the TruSeq DNA Sample preparation   kit (Illumina) with the following modifications. Fragmentation was   performed with a Covaris S2 using the program recommended in the kit   protocol except that the shearing duration was reduced to 100 seconds   and the fragmented DNA was subjected to a 1:0.8x Ampure (Beckman) prior   to subsequent steps. The resultant smear of fragments ranged from   100-2000 bp. Also, only 4 cycles of enrichment PCR were undertaken,   rather than the recommended 10 cycles, to minimize amplification bias.   Post-amplification the library minimum size had increased by   approximatedly 120 bp, as expected because of the addition of TruSeq   adapters to the initial fragments.  About 10% of a HiSeq2000 lane, 3.7   billion bases of sequence comprising 18.6 million read pairs were   generated. Insert sizes of the sequenced library molecules were obtained   from the ABySS-PE assembly at kmer60 to largely range from 120-500 bp