title: RADseq of Placopecten magellanicus: muscle
design: 1 ľg DNA, Sbf1 restriction enzyme, sheared with sonicator, individual barcodes appended, 20 individuals per sampling location pooled and sequenced on 1 lane, PCR cycles 98 °C for 30 seconds; 13 cycles of 98 °C for 30 seconds, 65 °C for 30 seconds, 72 °C for 30 seconds; 1 cycle of 72 °C for 5 minutes