title: GSM3462649: Muscle_F2 (mouse_BS); Mus musculus; Bisulfite-Seq
library_construction_protocol: Total RNA was isolated from tissues or cultured cells using chloroform and isopropanol following the manufacturer's protocol. Polyadenylated RNA was separated from total RNA using either GenElute mRNA miniprepKit (Sigma-Aldrich) or Oligo dT Magnetic Beads (Vazyme). Bisulfite treatment was performed using the EZ RNA methylation kit (Zymo Research) with significant modifications.