title: GSM3208983: NT 4-cell (to blastocyst) (WGBS-Seq); Mus musculus; Bisulfite-Seq
library_construction_protocol: For WGBS, 30-50 cells were used per reaction. All isolated cells were washed three times in 0.5% BSA-PBS solution to avoid possible contamination. The sequencing libraries were generated using the Pico Methyl-Seq Library Prep Kit following the manufacturer's instructions. Paired-end 150-bp sequencing was performed on a NovaSeq (Illumina) at Berry Genomics Corporation