title: GSM4476459: M. albugensis + A. laibachii Peeled Rep3; Arabidopsis thaliana; Albugo laibachii; Moesziomyces sp. 2020a; RNA-Seq
library_construction_protocol: Four weeks old A. thaliana plants were fixed and liquid latex was applied to the leaf surface. The latex was dried using the cold air option of a hair dryer, carefully peeled off  and immediately frozen in liquid nitrogen. Afterwards the frozen latex pieces were grinded with liquid nitrogen and the RNA was isolated by using Trizol® Reagent (Invitrogen) according to the manufacturer's instructions.DNA contaminations were removed by using Turbo DNA-Free™ Kit (Ambion). RNA libraries were prepared by the CCG (Cologne genome center) using standard Illumina protocols