title: 454 sequencing of Barley cv. Morax fragment library
design: Preparation and sequencing of the 454 sequencing library was essentially  performed according to manufactorer’s instructions (GS20 Shotgun DNA  Library Preparation Kit / Sequencing Kit, Roche Diagnostics, March  2007). About 4 µg of barley cv. Morex whole genome DNA were fragmented  for 1 min at 3 bar N2 by nebulizers to an average fragment length of 700  bp (determined on DNA-1000 LabChip, Agilent). The library was quantified  by RNA-1000 LabChip (Agilent) and the Quant-IT RiboGreen RNA assay  (Invitrogen). The reads analysed in this study were generated on a GS20  by two runs (two segments each) with 70x75 picotiterplates and 4 out of  8 segments ("titration") of a 40x75 picotiterplate.