title: RNA-Seq of gorilla: iPS
design: iPS cells were grown as described in (Marchetto et al., 2013) with the following modifications: Cells were cultured in the presence of the 3iL factors described in (Chan et al., 2013, PMID: 24315441). Upon optimization for primate iPS culture, the final conditions were: Recombinant Human Leukemia Inhibitory Factor (LIF) 0.4 ng/ml (ProSpec-Tany TechnoGene Ltd: cyt-644); BIO 80 nM, PD0325901 40 nM; Dorsomorphin 80 nM (Cayman Chemical: 13123, 13034, 11967). Trizol and TURBO DNase (Thermo Fisher) were used in RNA isolations. RNAs were further purified using Direct-zol RNA MiniPrep and RNA Clean &amp; Concentrator-5 (Zymo Research)