title: GSM2546274: Th2_AA_3; Mus musculus; miRNA-Seq
library_construction_protocol: 8,000-3,000,000 cells were lysed in Trizol LS (Life Technologies) and RNA isolated using a miRNAeasy Micro Kit (Qiagen). 5-50ng of total RNA from sorted and/or cultured cells were used to build miRNA libraries as previously described (PMID 23440203).