title: GSM2544688: Total (8WG16) mNET-Seq OH+; Homo sapiens; OTHER
library_construction_protocol: Nascent RNA was isolated using immunopurification of mammalian nascent elongating transcripts (mNET-IP) as described in Nojima 2016. For ChIP-seq: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with Tyrosine 1-p antibody. mNET-seq: Libraries were prepared as described in Nojima 2016; small RNA-seq library