title: small RNA-seq of Phytolacca americana leaf tissue
design: Libraries were prepared with the strand-specific SOLiD Total RNA-Seq Kit (Life Technologies, Burlington, ON, CA). Acrylamide gel purification of the 15–30?nt size fraction of RNA; ligation of primers; cDNA synthesis and acrylamide gel purification; PCR amplification (15?cycles) and column purification.