title: 454 sequencing of wastewater metagenome MallardCreek032007AerobicBasin-genomic genomic fragment library
design: PCR conditions: Initial denaturation of 94C for 5 minutes followed by cycling 20 rounds of 94C for 1 minute, 60C for 1 minute (dropping 1C every 2nd cycle) and 72C for 1 minute.  10 rounds of PCR cycling with annealing temp set to 55C. Final extension, 72C for 7 minutes.  Final dwell at 4C.