title: GSM3132757: WT_HFHC_K27ac_rep3; Mus musculus; ChIP-Seq
library_construction_protocol: Fresh mouse livers were crosslinked with 2 mM disuccinimidyl glutarate (DSG) for 30 min, followed by 1% formaldehyde for 10 min at room temperature. Nuclei were isolated, sonicated and incubated with magnetic bead-antibody complexes.  Briefly, DNA was end-repaired and ligated to stem-loop adaptors. Then DNA was PCR amplified with Illumina-compatible primers for 9 cycles and library fragments of 200 to 400 bp were selected using Ampure XP beads.