title: WGS of X. hellerii: muscle
design: Muscular tissue, was digested by  Protease K at room temperature for 1 hr. The lysate was then transferred to  2.0ml collection tubes. DNA isolation was performed by a QIAcube HT (Qiagen)  automated bio-sample isolation system, with reagent contained in QIAamp 96  DNA QIAcube HT Kit. The isolation system is equipped with a robotic arm with  8 pipettes. Each pipette is able to pick and eject pipette tips, self-clean,  and transfer liquids between wells/columns, or between master reservoirs and  wells/columns in standard 96-well plate formats. Each sample was  independently maintained throughout the isolation process. Concentrations of  DNA samples were measured using Qubit 2.0 fluorometer (Life Technologies,  Grand Island, NY, USA), and adjusted for sequencing library preparation. DNA  samples were forwarded for genome shotgun sequencing library preparation  using Illumina Nextera sequencing Library Prep Kit, followed by sequencing on  HiSeq 2000 (Illumina, Inc., San Diego, CA, USA) using 150 bp paired-end (PE)  sequencing strategy.