title: GSM4656193: Healthy_primary_shNTC_ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq was performed  as described in Corces et al. 20162. Briefly, cells were harvested and 50,000 cells pelleted prior to resuspension in 50 µl ATAC transposition reaction mix containing 2x Tagment DNA Buffer (Illumina), Tn5 transposase (Illumina) and 0.5 µl 1% Digitonin (Promega). DNA was purified using a MinElute Reaction Clean up kit (Qiagen). DNA was PCR amplified using Nextera custom primers and saturation of the PCR reaction avoided by monitoring the reaction to prevent overamplification as described in Buenrostro et al.3,4. Amplified DNA was subsequently purified using MinElute columns or Ampure Beads (Beckman Coulter) prior to validation. Libraries were sequenced in a pool of 12 indexed libraries using a NextSeq 500/550 High Output Kit v2 (150 cycles) for paired end sequencing (Illumina) at the Genomics Birmingham sequencing facility. ATAC-seq libraries were prepared essentially as described in Corces, M. R. et al. 2016 and in Buenrostro et al.2015. Libraries were sequenced in a pool of 12 indexed libraries using a NextSeq 500/550 High Output Kit v2 (150 cycles) for paired end sequencing (Illumina) at the Genomics Birmingham sequencing facility.