title: COLO829 associated blood-derive lymphoblast sequenced on Novaseq
design: "Libraries were prepared using the TruSeq DNA PCR-free Library Preparation Kit (Illumina) with 1ug DNA input following Illumina's recommended protocol3, with minor modifications as described. Intact genomic DNA was concentration normalized and sheared using the Covaris LE220 sonicator to a target size of 450bp. After cleanup and end-repair, an additional double-sided bead-based size selection was added to produce sequencing libraries with highly consistent insert sizes. This was followed by A-tailing, ligation of Illumina DNA Adapter Plate (DAP) adapters and two post-ligation bead-based library cleanups. Final libraries were run on the Fragment Analyzer to assess their size distribution and quantified by qPCR with adapter specific primers (Kapa Biosystems). The libraries were pooled together based on expected final coverage and sequenced across multiple flow cell lanes. Whole genome sequencing was performed on the NovaSeq (NSCS 1.3.1; RTA v3.3.3)  at 2x150 bp read length, using v1 S2/S4 300-cycle  reagents."