title: RNA_Seq of Homo sapiens: infant female blood: Sample CRC5332
design: Total RNA was extracted using the Zymo research Quick-RNA miniprep kit (Cat# 11-327). ERCC spike-in RNAs (Cat#4456740 Illumina) were added to 1mg of total RNA as recommended by the manufacturer, and ribosomal RNA depletion was performed using the RiboZero gold kit (Cat#MRZG126). cDNA libraries were prepared with 20ng of rRNA-depleted RNA using the NEBNext Ultradirectional RNA Lib Prep Kit (Cat #E74205) and NEBNext Multiplex Oligos (Cat#E73355).  Library quality was assessed on the Agilent 2100 Bioanalyzer and 75bp paired-end reads were obtained in a single multiplexed run on an Illumina NextSeq500 system with mid capacity cartridge in the UVA Biomolecular Core Facility. 