title: 454 sequencing of Microctonus aethiopoides venom gland transcript fragment library
design:              No library was constructed. cDNA was simply amplified and sequenced directly. Sequencing was undertaken by Roche Corporation using a GS20 DNA sequencer. The cDNA used for sequencing was 6             micrograms amplified cDNA derived from venom RNA prepared from M. aethiopoides venom glands dissected and purified. The SMART cDNA preparation protocol (Clonetech) was followed according             to the manufacturers instructions using 3 microliters of RNA solution as starting material and 26 cycles of amplification. To obtain the quantity of cDNA required for sequencing (6             micrograms) the cDNA protocol was repeated three times and the amplification products were pooled.         