title: HAT-seq bulk sequencing of human tissues
design: Multiplexed HAT-seq libraries were constructed using KAPA LTP Library Preparation Kit (KAPA Biosystems, KK8232) with substantial modifications. Briefly, genomic DNA were sonicated and constructed into WGS libraries without size selection. Adaptor ligated DNA were amplified with one primer specific to L1Hs (L1Hs-AC-28) and another primer annealing to adaptor. Enriched libraries were barcoded by additional five cycles of PCR and size selected with Pippin Prep (Sage Science). Pooled HAT-seq libraries were single-end sequenced (90bp) at BGI (Shenzhen, China).