title: GSM2455323: TruSeq Col-0 Rep2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA was extracted from inflorescence tissue using the TRIzol exctraction method. To generate total RNA-seq libraries, RNA was subjected to ribodepletion with Ribo Zero (Illumina) and libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina). RNA 3’end libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) adjusted for longer insert lengths by diluting the second strand synthesis buffer with water in a ratio 1:1.