title: GSM2722416: Extracted RNA from Ki67-RFP+ cells of hearts of Ki67TagRFP mice 14 days post-MI surgery, replicate 1_bulk RNA; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted into Trizol (Life Technologies) and total RNA was isolated according to the manufacturer's instructions, with the following alterations. RNA was precipitated overnight at -20 °C, with 2.5 ug GlycoBlue (Life Technologies). RNA was processed using the previously described CEL-seq technique, with the indicated modifications. Libraries were sequenced on an Illumina NextSeq500 using 75bp paired end sequencing.