title: meCLIP of EIF4A3: SuperScript III (readthrough)
design: UV-crosslinking and immunoprecipitation using anti-HA antibody on CRISPR-tagged HeLa cells. Ligation of 5'-biotinylated linker. RT performed using SuperScript III followed by NextSeq Illumina sequencing. Reads without the 5'-linker were obtained and named "readthrough" reads.