title: Metagneome of lab-scale EBPR activated sludge
design: AS samples were fixed with ethanol (1:1 in volume) on site and stored at -20  until DNA extraction. During DNA extraction, biomass was collected by centrifugation. Then DNA were extracted by using the FastDNATM Spin Kit for Soil (MP Biomedicals, US) according to the instruction manual. For DNA from isolates and AS samples, Illumina Truseq DNA PCR free (insert fragment 350 bp) libraries were constructed before paired-end sequencing on the Illumina HiSeq X10 platform (2  150 bp).