title: Gene content and virtual gene order of barley chromosome 1H - Reads run 6
design: Intact mitotic chromosomes were isolated by flow cytometric sorting and the purity of the obtained chromosome suspension was determined by fluorescence in situ hybridization (FISH) essentially as described previously (Suchankova et al., 2006). The DNA of sorted chromosomes was purified and amplified by multiple displacement amplification (MDA) as described by Šimková et al. (Simkova et al., 2008).