title: GSM2545685: prp8-V1860N; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells were harvested by filtration, flash frozen in liquid Nitrogen and RNA was extracted using hot acid-phenol, chloroform extraction. 5ug total RNA was used as input for Illumina Ribo-Zero Gold kit (Yeast) followed by treatment with RNaseR (Epicentre). RNA libraries were prepared for sequencing using altered double strand cDNA synthesis conditions followed by standard Illumina TruSeq RNA-seq protocol with modified cleanup strategy between steps.