title: Illumina sequencing of A549 total reverse-crosslinked chromatin after 1h, 0.02% Ethanol Treatment
design: A549 cells were treated for 1h with either 100nM Dexamethasone, or an equal volume of ethanol as a vehicle control, as indicated.  Chromatin was formaldehyde crosslinked, sheared, followed by heat reversal of crosslinks. Purified DNA was made into Illumina sequencing libraries by addition of Illumina linkers to repaired ends, PCR amplification, gel extration of fragments ~150-250bp (excluding linkers), and a second PCR amplification.  Purified DNA was used for cluster generation and sequencing using the Illumina 1G Genome Analyzer.