title: HiSeq X Ten paired end sequencing; Whole genome sequence (VarGoats 266 dataset)
library_construction_protocol: Next Generation sequencing libraries are prepared using Illumina SeqLab specific TruSeq Nano High Throughput library preparation kits in conjunction with the Hamilton MicroLab STAR and Clarity LIMS X Edition. The gDNA samples are normalised to the concentration and volume required for the Illumina TruSeq Nano library preparation kits, then sheared to a 450bp mean insert size using a Covaris LE220 focused-ultrasonicator. The inserts are ligated with blunt ended, Atailed, size selected, TruSeq adapters and enriched using 8 cycles of PCR amplification. The libraries are evaluated for mean peak size and quantity using the Caliper GX Touch with a HT DNA 1k/12K/HI SENS LabChip and HT DNA HI SENS Reagent Kit. The libraries are normalised to 5nM using the GX data and the actual concentration was established using a Roche LightCycler 480 and a Kapa Illumina Library Quantification kit and Standards.