title: NextSeq 500 paired end sequencing; Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO
design: Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO
library_construction_protocol: Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose.  RNA was extracted in Trizol by phenol-chloroform method.