title: GSM4406874: Splenocytes - Wtm_4; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq: Cell lysates were frozen in RLT plus buffer at -80oC prior to processing. ATAC-seq: cells were processed immediately For RNA-Seq: total RNA was extracted using the RNEasy plus micro kit (Qiagen).  Cells were reuspended in RLT plus buffer and stored briefly at – 80 °C prior to extraction. All reagents, plastic ware, and supplies used were sterile, nuclease free, and of molecular biology grade. RNA purity (A260/A280>1.80) and concentration were determined using nanodrop spectrophotometer (Thermo Fisher scientific, Delaware USA). RNA integrity was studied using the 2100 Bioanalyzer System (Agilent) and RNA 6000 Nano Kit. A RNA Integrity Number (RIN) of 6.3 was the lowest cut-off for RNA Sequencing.  For ATAC-seq, cells were processed immediately as described (Corces et al (2017), Nat. Methods, 14, 959) RNA-seq libraries were constructed using the Stranded total RNA-seq v2-pico kit (Takara) and ATAC-seq libraries were constructed as described (Corces et al (2017), Nat. Methods, 14, 959)