title: RNA-Seq of Avocado
design: Poly(A)-RNA was isolated using oligo(dT) magnetic beads (Invitrogen  Dynabeads). 0.5-1 g of poly(A)RNA were then converted into  full-length-enriched double stranded cDNA using the Mint-2 cDNA synthesis kit  and following the manufacturers instructions (Evrogen). The double stranded  cDNA was then normalized using the DSN-based Trimmer-2 cDNA normalization kit  and following the manufacturers instructions (Evrogen). The normalized cDNA  libraries were then sheared into ~300 bp fragments with a sonicator (Bioruptor,  Diagenode) and indexed with adaptors using technology NEBNext DNA Library  Prep Master Mix Set for Illumina