title: 16SrDNA of soil: glucose amended and 42 days incubated red ferralsol
design: The 16S rRNA gene V4 variable region PCR primers 515/806 with barcode on the forward primer  were used in a 28 cycle PCR. Multiple samples are pooled together (e.g., 100 samples) in equal proportions based on their molecular weight and DNA concentrations. Pooled samples are purified using calibrated Ampure XP beads. Then the pooled and purified PCR product is used to prepare illumina DNA library. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq following the manufacturerÕs guidelines.