title: GSM3462404: Etv5-3xFlag Knock-in (KI)-32 2i; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP-DNA was eluted with Qiagen MinElute PCR purification kit Ev5-ChIP-Seq libraries were generated   using NEB Next ChIP-Seq Library Prep Master Mix Set for Illumina (NEB. For H3K4me3 ChIP-seq libraries DNA samples were prepared for sequencing by end repair of 20 ng DNA as measured by Qubit (Invitrogen). Adaptors were ligated to DNA fragments, followed by size selection (∼300 bp) and 14 cycles of PCR amplification. Cluster generation and sequencing (36 bp) was performed with the Illumina Genome Analyzer IIx (GAIIx) platform according to standard Illumina protocols