title: GSM4656221: Th1 Polarized CD4 T-Cells; Mus musculus; RNA-Seq
library_construction_protocol: Purified populations of Naïve, Memory and effector CD4 T-cells were spun down in FACS medium, washed and RNA was extracted. Purified RNA was obtained using QIAGEN RNeasy Micro kit or Zymo-research Direct-zol RNA Microprep kit. mRNA for RNAseq analysis was purified using PolyA+ selection and processed by the Yale Center for Genome Analysis using standard methodology using Illumina TruSeq mRNA-Seq Sample Prep Kits, library quality and concentration assayed using a Bioanalyzer, RNA was sequenced on a HiSeq2500 with 75bp pair-ended reads.