title: Sequencing of 3'-end of transcripts of a normalized grape berry library
design: The cDNA libraries were prepared by Eurofins MWG Operon, Ebersberg, Germany (www.eurofinsdna.com) in cooperation with Vertis Biotechnologie AG, Freising, Germany. In short: After enrichment of polyA  RNA, the first strand cDNA was synthesized using an oligo(dT)-adapter primer. After purification of the first strand cDNA, second strand cDNA synthesis was performed using a random (N)6-adapter primer. The cDNA was amplified with 15 cycles of PCR using a high fidelity DNA polymerase. Frangments in the size range of 450-550 bp were eluted from preparative agarose gels. An aliquot of the size fractionated cDNA was analyzed on a 1.5% agarose gel.