title: WXS for  Mus musculus 12 months male kidney non-induced
design: Samples  were collected from 5 thyroid tumors and 5 kidneys. Genomic DNA extraction  was performed with the All prep DNA/RNA kit (Qiagen, Hilden, Germany).  Genomic DNA was sheared via sonication using a S220 focused-ultrasonicator  (Covaris, Woburn, MA). DNA libraries were constructed using the SureSelectXT  Mouse All Exon kit (Agilent Technologies, Santa Clara, CA), according to the  manufacturers recommendations. The constructed libraries were sequenced  using Illuminas High Output Kit (150 cycles) on a NextSeq500 (Illumina, San  Diego, USA) with an average depth of 140x for tumor and 70x for normal  (kidney) samples.