title: 16S RNA-Seq of upper gastrointestinal carcinoma microbiota
design: DNA was isolated from UGI tract biopsies and mucosal specimens by using the MOBIO PowerSoil DNA Isolation Kit. The V4 region of the 16S rRNA gene was amplified using the universal bacterial primer set of 515-Forward and 806-Reverse. Using Illumina 11 cheQ f