title: GSM4155855: H3K4me2_2h_r2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 15 minutes at RT with mild shaking. Crosslinking was halted with 125 mM Glycine for 5 minutes. Cells were washed with PBS and the pellet was flash frozen in liquid nitrogen. Cells were lysed by bead beating, the chromatin was sheared by sonication, and protein-DNA fragments of interest were isolated with a specific antibody and magnetic protein A Dynabeads. Libraries were prepared as instructed by the ThruPLEX DNA-seq Kit (R400427, Takara). 0.5-1 ng of starting material was used with 11 rounds of PCR. AMPure XP beads (A63881, Beckman Coulter) were used to select fragments between 200-500 bp.