title: GSM2877830: Primary_follicle_A18O; Homo sapiens; RNA-Seq
library_construction_protocol: Each oocyte and 10 randomly selected granulosa cells were transferred into lysis buffer using a mouth pipette. The reverse transcription reaction was performed on the whole-cell lysate and terminal deoxynucleotidyl transferase was used to add a poly A tail to the 3′ end of the first-strand cDNAs, and then 20 cycles of PCR were performed to amplify the single-cell cDNA RNA libraries were prepared according to instructions of Kappa Hyper Prep Kit (Kappa Biosystems).