title: WGS of Strix occidentalis caurina: adult female blood
design: We extracted genomic DNA from blood using a DNeasy Blood & Tissue Kit (Qiagen). We sheared 4,460 ng genomic DNA in 130 µL in a microTUBE AFA Fiber Pre-Slit Snap-Cap tube (Covaris) using a M220 focused-ultrasonicator (Covaris) targeting 550 bp as the center of the fragment distribution. We used peak incident power 50 W, 20% duty factor, 200 cycles per burst, and 45 s treatment time at 20°C. We then removed small fragments and concentrated the sheared material using a DNA Clean & Concentrator -5 kit (Zymo Research). We next constructed a genomic library by using a TruSeq DNA PCR-Free Library kit (Illumina) and following the manufacturer's protocol, including the use of bead-based size selection to remove large and small DNA fragments in succession to target a mean fragment size of 550 bp. We pooled the final library with two other libraries equimolarly and we obtained 350 bp read 1 and 250 bp read 2 for a total of 600 bp of paired-end read data from one lane (approximately 1/3 of one lane for this library) using a 600-cycle MiSeq Reagent Kit v3 on a MiSeq (Illumina).