title: mouse adult cardiac myocyte cells rep1: noncoding RNAseq
design: Total RNA was isolated from FACS sorted viable cardiomyocytes from one adult heart using RNeasy Micro Kit (Qiagen) including on-column DNAse digestion. Ribosomal RNA was depleted using the Low Input RiboMinus Eukaryote System v2 (Life technologies). Depleted RNA fragmented to approximately 200 bp fragments and was used for library construction (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). Size selection was performed with AMPure® XP Beads (Beckman Coulter) according to the NEBNext® Ultra RNA Library Prep Kit manual. 15 PCR cycles were used for amplification.