title: amplicon-seq of treponema pallidum: X-4 isolate
design: The genome sequencing strategy of the X-4 isolate was based on the pooled segment genome sequencing (PSGS) method as reported previously. Briefly, X-4 genomic DNA was amplified using treponemal specific primers (n=278) to obtain overlapping PCR product.To ensure separate sequencing of paralogous gene, all PCR products were purified and mixed equimolar amounts into four distinct pools.