title: Transcriptome sequencing of 02428-2x (diploid rice) anthers, the anthers at the meiosis stage
design: The quantity and purity of total RNA were analysis by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 8. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, CA, USA). Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to construct the final cDNA library according to the manuscript of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA). Finally, we performed the paired-end sequencing with the average insert size of 300 bp (±50 bp) on an Illumina Hiseq2000 (LC Sciences, USA) following the vendor’s recommended protocol.