title: meCLIP of EIF4A3: AffinityScript (readthrough)
design: UV-crosslinking and immunoprecipitation using anti-HA antibody on CRISPR-tagged HeLa cells. Ligation of 5'-biotinylated linker. RT performed using AffinityScript followed by NextSeq Illumina sequencing. Reads with the 5'-linker were obtained and named "read-through" reads.