title: GSM2977231: Adult-male6-WTTSseq; Xenopus tropicalis; RNA-Seq
library_construction_protocol: WTTS-seq libraries were constructed based on our protocols. The WTTS-seq method includes several major steps: fragmentation, poly(A)+ RNA enrichment, 1st strand cDNA synthesis, size selection and PCR amplification Total RNA was harvested using Trizol reagent.