title: GSM3729038: Untreated FRT cells, biological replicate 3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Extraction of total RNA was performed according to the manufacturer's instructions (RNeasy Mini Kit Cat No./ID: 74106 (250), Quiagen). RNA extract was precisely quantified and mixed at 50ng/5 µL Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit  (Lexogen, Vienna, Austria) according to manufacturer's instructions The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the NovaseqNovaSeq 6000 Sequencing System (Illumina) with a read length of 100bp