title: SBRRIA Low Nitrogen Content LNC
design: A random collection of biomass samples from one of the vertical PU foams of SBRRIA was performed at the end of both Low Nitrogen Content (LNC) and High Nitrogen Content (HNC) conditions. DNA extraction was based on the procedure described by Griffiths et al. (2000) . The primers set 341F and 805R were used for V3 and V4 hypervariable regions of the 16S rRNA.