title: GSM2876158: C3-rep3 [miRNA]; Litopenaeus vannamei; miRNA-Seq
library_construction_protocol: Hepatopancreas were removed, immediately snap frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. The library preparations were sequenced at the Novogene Bioinformatics Institute (Beijing, China) on an Illumina HiSeq 2500 platform and 50 bp single-end reads were generated after the clustering of the index-coded samples, performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumina), according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols