title: AB 5500xl Genetic Analyzer paired end sequencing; RNAseq of PBMC from HIDS patients
design: RNAseq of PBMC from HIDS patients
library_construction_protocol: Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from blood samples of VPER and VRIS using Ficoll-Paque™. PBMC  cells (106 cells) were seeded in 24-well plates in RPMI1640 supplemented with fetal calf serum (FCS), penicillin, streptomycin, amphotericin B (all from Invitrogen) and stimulated with LPS (1 µg/mL;  from Salmonella Abortus equi ; Sigma, Saint-Quentin-Fallavier, France) for 3 h or 6 h at 37 °C in a humidified atmosphere containing 5% CO2. The control condition without LPS stimulation is named mi. Description of the sample naming: lps: stimualtion with lps - mi: no stimulation - 3h/6h: lps stimulation for 3 or 6 hours - _1 or _2: replicate 1 or 2 - VPER: subject VPER - VRIS: subject VRIS Trizol SOLiD Total RNA-Seq Kit