title: DNA-Sequencing of seawater from Monterey Bay, CA: 18S target markers
design: This protocol is aimed at amplifying the 18S rRNA hypervariable region 9 (18S V9) in eukaryotes with a focus on microbial eukaryotes. The primers (1391F,  EukBr, and 1391_Blocker) utilized in this protocol are based on the primer utilized in Amaral-Zettler et al 2009 and the Earth Microbiome Project (EMP).