title: Miseq 18S Gregarinasina community: seminal vesicles
design: Two different regions of gregarine SSU (region of 200bp and 477bp) were generated by PCR with the use of specific primers.  The amplicon sequencing data were processed with SEED v2.1. Pair-end reads were joined using fastq-join. Chimeric sequences were detected using algorithm UCHIME, deleted and clustered using UPARSE at a 97% similarity level, both of which were conducted with USEARCH 8.1.1861. Singletons were excluded from all analyses.     So, we obtained two files with raw data, which were joined at first. Then we sorted sequences according to their primers and barcodes to appropriate samples. Like this we obtained all sequences belonging to the right sample and these were submitted.