title: GSM2720204: HTE RNA-seq rep3; Homo sapiens; RNA-Seq
library_construction_protocol: TruSeq Stranded Total RNA Library Prep kit with Ribo-Zero Gold genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. Total RNA is extracted by Trizol (HBE) or RNeasy (HTE) DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012. RNA seq libraries prepared by standard Illumina protocols.