title: GSM3462790: thoracic aortic aneurysm plasma sample 5; Homo sapiens; miRNA-Seq
library_construction_protocol: RNA from tissues and plasma were extracted using Mirvana Ambion and Mirvana Paris kit, respectively. cDNA libraries for sequencing were prepared using NEXTflex Small RNA-Seq v3 kit. Ligation reactions of 3′ and 5′ adapters were performed using 1,6 µg of total RNA or 10,5 µL of each RNA samples isolated from aortic tissues or blood plasma, respectively. 18 or 23 PCR cycles of amplification were performed to generate cDNA from tissue or plasma mRNAs, respectively.