title: Digital gene expression analysis reveals two sets genes associated with hexythiazox resistance in Panonychus citri (Acari: Tetranychidae)
design: Digital gene expression analysis reveals genes associated with hexythiazox resistance in citrus red mite, Panonychus citri
library_construction_protocol: A colony of Citrus red mite, Panonychus citri (McGregor), was collected from citrange next to citrus orchard fences,which had never been exposed to acaricides for many years. These mites were reared in citrange for 4 years under acaricide-free conditions (25 +/-1 degree C, 80 +/-5% RH and photoperiod of 14 hours light: 10 hours darkness) and considered as a susceptible strain.The susceptible strain was treated with hexythiazox (about 70% population mortality rate in every spraying) and continuously screened for 28 generations for 4 years, and the resultant strain was regarded as the resistant strain.The mixture of eggs, larvae and adults was collected in a PE (PerkinElmer) tube as one sample for SS and RS, respectively.  A collection of 200 individuals was used for each of the sample (SS and RS) for total RNA extraction