title: Mus musculus Raw sequence reads
design: Total RNA was isolated from two WT and two -/- hearts for each group.  RNA was extracted using Trizol regent and RNA-seq library was prepared according to manufacturer s instruction (Illumina, http://www.illumina.com).  The cDNA library was sequenced on the Illumina sequencing platform (Illumina HiSeq PE150).  Sequencing reads in FASTQ format were mapped to the reference genome (GRCm38/mm10), and splice junctions were identified using TopHat.  Replicate MATS (rMATS) was used to analyze AS.