title: GSM4407575: flag.PBS.rep12; Mus musculus; ChIP-Seq
library_construction_protocol: On day of harvest, mice were euthanized by CO2 inhalation. Livers were harvested and immediately snap frozen using liquid N2.  Livers were then treated for chromatin extraction as described in Savic et al, 2013. Briefly, following fixation, livers were rocked at 4˚C in 10 ml Farnham Lysis Buffer for 10 minutes followed by centrifugation. Pellets were resuspended in 4 mL RIPA buffer. 1 mL RIPA and 1 mL RIPA containing resuspended liver was placed into a 15 mL tube with 800 mg of Diagenode sonication beads (Diagenode, C01020031). This tube was sonicated for 30 cycles of 30s on and 30s off using a Bioruptor Pico. ChIP-seq libraries were prepared using a Kapa Hyper Prep kit.
attributes.tag: GEO Accession
attributes.value: GSM4407575