title: GSM3819853: wt_SMC_16wk_citeseq_antibody; Mus musculus; RNA-Seq
library_construction_protocol: Cells were enzymatically dissociated from the aortic root and ascending aorta (to the level of the brachiocephalic artery) of experimental mice Cells were captured on a 10x Chromium instrument (10x Genomics, Pleasanton, CA) using either the V2 or V3 chemistry for 3' whole transcriptome analysis.  Manufacturer's instructions for GEM (Gelbead EMulsion) generation, downstream reverse transcription, cDNA isolation were followed. Prior to library construction, cDNA was separated using size selection into mRNA and antibody barcode fractions and PCR amplification and library preparation were conducted separately. For the mRNA fraction, PCR amplification and library preparation were performed following the manufacturer's instructions. For the antibody barcode fraction, PCR and library construction was performed according to the standard CITE-seq protocol (https://cite-seq.com/protocol/). Both library fractions were pooled together prior to sequencing.