title: Amplicon-Seq of BP15
design: Microbial community DNA was extracted from the anaerobic digester                 sample using a CTAB-based method (modified from Griffiths et al. 2000) and then 16S                 amplicons were generated with the primerset 515YF (5´-GTGCCAGCMGCCGCGGTAA-3´) and                 806R (5´-GGACTACHVGGGTWTCTAAT-3´) as suggested by Klindworth et al. (2013).                 Sequencing was performed by Mcirosynth AG (Balgach, Switzerland) with the                 MiSeq-technology. Paired-end reads were merged to a single read.