title: GSM3904256: Group2-Asc-TMDKOAsc-2; mouse gut metagenome; OTHER
library_construction_protocol: DNA extraction and sequencing of the 16S ribosomal RNA gene was performed for mouse fecal samples as previously described (Tong et. al, PMID: 25367129). In brief, bacterial DNA was extracted using the ZymoBIOMICS DNA kit (cat#D4300) with bead beating. The V4 region of the 16S gene was amplified and barcoded using 515f/806r primers then 250x2 bp sequencing was performed on an Illumina HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM3904256