title: GSM3672442: WTF3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from liver tissues was isolated using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) and then the quality of isolated RNA was first assessed using the conventional A260/280 ratio and A260/230 ratio measurement (SpectraMax i3x; Molecular Devices, Sunnyvale, CA, USA) which were all satisfactory. In addition, RNA Integrity Number (also known as RIN) was assessed using the RNA R6K assay for the Agilent 2200 TapeStation (Agilent Technology, Santa Clara, CA, USA) in which RINs ranged between 8.5 and 9.5 hence satisfactory. Illumina TruSeq RNA Library Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
attributes.tag: GEO Accession
attributes.value: GSM3672442