title: GSM2983186: SIIIRTMn +CMC; Homo sapiens; OTHER
library_construction_protocol: Total RNA was isolated from confluent 100-mm plates of HEK293T cells by Trizol extraction (Life Technologies, 15596-018) and size-selected for RNAs over 200 nucleotides long with the mirVana miRNA isolation kit (Life Technologies, AM1561). RNA was fragmented, CMC- or mock-treated, end-repaired, ligated to a 3' adapter, and reverse transcribed with one of three conditions. The cDNA was then size-selected by PAGE, circularized, amplified with barcodes, size-selected by PAGE, and sequenced in a single lane.
attributes.tag: GEO Accession
attributes.value: GSM2983186