title: GSM4481481: KO_1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were isolated by sorting using an Aria sorter and total RNA purified using the RNeasy Micro kit (Qiagen). RNA quality was assessed with an Agilent 2100 Bioanalyzer (RNA integrity number>9 for all samples) Barcoded sequencing libraries were generated with 100ng of RNA with an Ovation RNAseq System V2 (Nugen), KAPA Hyper Prep Kit for Illumina (KAPA Biosystems), and NEXTflex DNA barcodes (Bioo Scientific). Single-end sequencing was performed on an Illumina HiSeq 4000 (UCSF Center for Advanced Technology)
attributes.tag: GEO Accession
attributes.value: GSM4481481