title: Illumina HiSeq 2500 sequencing; ChIP-seq of NuRD components and pluripotency factors in wild-type, Mbd3 and Sall4/1 mutant embryonic stem cells
design: ChIP-seq of NuRD components and pluripotency factors in wild-type, Mbd3 and Sall4/1 mutant embryonic stem cells
library_construction_protocol: ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. Cells were fixed using 1% formaldehyde for 10 min at room temperature and fixation quenched with 150 mM glycine. Chromatin was sheared to an average fragment size between 200 and 300 bp using a Bioruptor sonication instrument (Diagenode). Immunoprecipitation was performed using antibodies against endogenous proteins or the FLAG tag. Libraries were prepared with the NEXTflex Rapid Illumina DNA-seq Library Prep Kit (Bioo Scientific).
attributes.tag: Experimental Factor: 2lox
attributes.value: cell line
attributes.tag: Experimental Factor: Mbd3flox/-
attributes.value: genotype
attributes.tag: Experimental Factor: Esrrb
attributes.value: immunoprecipitate