title: GSM3671086: DMSO replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA extractions for RNA-seq were performed using the mirVana kit (Applied Biosystems). RNA concentration was measured using the Qubit RNA broad range assay in the Qubit Flurometer (Invitrogen) and RNA integrity was determined with Eukaryote Total RNA Nano Series II ChIP on a 2100 Bioanalyzer (Agilent). RNA-seq libraries were prepared using TruSeq RNA sample preparation kit (Illumina) Oligo-dT purified mRNA was fragmented and subjected to first and second strand cDNA synthesis. cDNA fragments were blunt-ended, ligated to Illumina adaptors and PCR amplified to enrich for the fragments ligated to adaptors. The resulting cDNA libraries were verified and quantified on Agilent Bioanalyzer before they were sequenced (paired-end 2x75 bp).
attributes.tag: GEO Accession
attributes.value: GSM3671086