title: GSM4661310: Female ESC 1; Mus musculus; Bisulfite-Seq
library_construction_protocol: Cells were collected and isolated their genomic DNA by QIAamp DNA Micro Kit (QIAGEN) DNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and DNA concentration was measured using Qubit® DNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). A total amount of 1.5 μg DNA spiked with moderate lambda DNA was handled by MspI, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer's instructions. Then these DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research), before the resulting single-strand DNA fragments were PCR amplified using KAPA HiFi HotStart Uracil and ReadyMix (2X). Library concentration was quantified by Qubit® 2.0 Flurometer and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system. The library preparations were sequenced on an Illumina Novaseq platform and 125bp/150bp paired-end reads generated. Image analysis and base calling were performed with Illumina CASAVA pipeline, and finally 125bp/150bp paired-end reads were generated.
attributes.tag: GEO Accession
attributes.value: GSM4661310