title: Nodule metagenome nifH sequencing of AG 5332_IR plot replicate 1
design: Nodules were cleaned and sterilized prior to DNA extraction.  NifH gene was amplified using PolF and PolR from (Poly et. al 2001). Library was prepared according to the Illumina Metagenome MiSeq sequencing protocol. Sequencing with performed using the 500 cycle v2 kit.