title: GSM4658357: 592T untreated_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: TRIzol (Invitrogen) Library preparation, sequencing, and basic analysis were conducted by MACROGEN Company (Republic of Korea). In order to construct cDNA libraries with the TruSeq Stranded mRNA LT Sample Prep Kit, total RNA was used. PolyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100 nt paired-end sequencing by Illumina NovaSeq 6000 were performed. The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using an Agilent Technologies 2100 Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM4658357