title: GSM4409132: cDC1_D7_rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Samples were prepared according to Buenrostro et al., Nature Methods 2015 for ATAC-seq or Corces et al., Nature Methods 2017 for Omni-ATAC-seq. Briefly, 5,000-50,000 sorted cells were centrifuged, washed, and resuspended in lysis buffer to extract nuclei. Nuclei were centrifuged and resuspended in Tn5 transposition reaction mix for 30 min at 37ºC. Reactions were scaled according to number of cells. Transposed DNA was column purified and stored at -20ºC. Samples were PCR amplified. In accordance with published protocols (Buenrostro et al., Nature Methods 2015; Corces et al., Nature Methods 2017) qPCR was used to determine the optimal number of PCR cycles to reduce GC and size bias. Samples were barcoded using custom primers as described (Buenrostro et al., Nature Methods 2013). Libraries were quantified with an Agilent Bioanalyzer and pooled for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4409132