title: GSM4259357: TNBC brain met scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: The freshly dissected brain tissue was cut up and then enzymatically dissociated to obtain a suspension of cells using a Tumor Dissociation Kit (Miltenyi Biotec) by incubating at 37degree for 15 minutes. Dissociated single cells were pelleted by centrifuged at 130g for 10 minutes after filtering through a 20 μm cell strainer. Single-cell libraries were prepared using the Chromium 3′ v2 platform (10X Genomics) following the manufacturer's protocol
attributes.tag: GEO Accession
attributes.value: GSM4259357