title: GSM4658305: Drosophila wing disc Control Ubx-GAL4>DamPol2 rep1; Drosophila melanogaster; OTHER
library_construction_protocol: 30 wing discs were dissected in 1xPBS, were frozen at -80˚C, thawed, 40ul of 500mM EDTA, 180ul of ATL buffer, and 20ul Proteinase K added, mixed gently and incubated for 56˚C overnight, cooled to RT and 20ul of RNAase (12.5ul/ul) added and incubated for 2 mins. 400ul of a 1:1 mix of Buffer AL and 100% ethanol was added and mixed gently, before processing through the DNeasy kit spin columns. The genomic DNA was then digested overnight with DpnI, cleaned up with a Qiagen PCR Purification kit, and DamID Adapters blunt ligated with T4 ligase, digested again with DpnII, and then adapter-ligated fragments PCR amplified using DamID primers and Advantage PCR kit DNA polymerase (Clontech). Adapters were then removed with AlwI digestion, Libraries were prepared using standard Illumina DNA shotgun library preparation with bead size selection protocol
attributes.tag: GEO Accession
attributes.value: GSM4658305