title: TUG-1 4C sequencing
design: cells are crosslinked with 1% formaldehyde 10 mins at room temperature to preserve three-dimensional nuclear architecture. An enzyme recognizing a 6-bp sequence (Hind III) was used as the primary restriction endonuclease to digest crosslinked chromatin. Then the digested chromatin was ligated using the T4 DNA Ligase. The digested and ligated chromatin is then de-crosslinked and submitted to a second restriction digest using an endonuclease recognizing a 4-bp sequence (CviQI) to reduce fragments sizes. PCR reactions were performed using TUG1-specific primers harbouring Illumina adapter sequences to amplify the genomic DNA fragments ligated to TUG1.