title: GSM4106035: patient K [C163_CD_M3]; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in a solution of TCL (Qiagen) and 1% 2-Mercaptoethanol.  RNA samples were   isolated using the Qiagen RNeasy Mini kit following the manufacturers recommendations. RNA quality check was done using Agilent RNA Nano 6000 chip (Agilent Technologies). Whole Transcriptome amplification (WTA) was performed using a modified SMART-Seq2 protocol, with Maxima Reverse Transcriptase (Life Technologies) used in place of Superscript II. Whole transcriptome amplification (WTA) products were cleaned with Agencourt XP DNA beads and 70% ethanol (Beckman Coulter, Brea, CA). Illumina sequencing libraries were prepared using Nextera XT (Illumina, San Diego, CA). The library of each sample was pooled together, and cleaned with two rounds of 0.8x DNA SPRIs (Beckman Coulter). Library quality was assessed with a high sensitivity DNA chip (Agilent) and quantified with a high sensitivity dsDNA Quant Kit (Life Technologies). Samples were sequenced on an Illumina NextSeq 500 instrument using the 75 cycles NextSeq 500/550 High Output Kit v2 (37base pairs paired-end reads).
attributes.tag: GEO Accession
attributes.value: GSM4106035