title: GSM1859903: A4-3; Aspergillus nidulans; RNA-Seq
library_construction_protocol: Three biological replicates were analyzed for each strain. All strains were cultured in agitating liquid-submerged medium (vegetative growth) for 18 h, and then shifted to an air-exposed medium to induce development. All samples were collected at time point 12 h following developmental induction, total RNA was extracted usiing TRIzol kit, then submitted for library preparation and sequencing. Strand specific library was prepared from total RNA using Illumina TruSeq Strand specific RNA sample preparation system. Briefly, mRNA was extracted from total RNA using polyA selection, followed by RNA fragmentation. Strand specific library was constructed by first-strand cDNA synthesis using random primers, followed by sample cleanup and the second-strand synthesis using DNA Polymerase I and RNase H. A single 'A' base was added to the cDNA fragments followed by ligation of the adapters. Final cDNA library was obtained by further purification and enrichment with PCR, and the quality was checked using Bioanalyzer 2100. The library was sequenced (PE100bp) using the Illumina HiSeq2500, with final of over 10 million high quality reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM1859903