title: RNA-seq of Sophora alopecuroides: CK_3
design: Total RNA was extracted using TRIzol reagent (Invitrogen, Burlington, Canada) according to the manufacturers protocol. The quality and purity of RNA samples were assessed using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA) with the RNA integrity number (RIN) > 7.0. Magnetic beads with Oligo (dT) (Invitrogen, Burlington, Canada) were used to isolate mRNA from 10 g total RNA. Following purification, the mRNA was cleaved into short fragments with divalent cations under elevated temperature. Then, the cleaved RNA fragments were reverse transcribed to create the final cDNA library in accordance with the protocol for the RNA-Seq sample preparation kit (Illumina, San Diego, USA). We performed the paired-end sequencing on an Illumina HiSeq Xten  following the vendors protocol.