title: GSM3371765: 1_Control; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from four lymphoblastoid cell lines including one derived from healthy control subject, one WAGR patient with C11orf46 gene intact and two WAGR patients with C11orf46 gene deletions using a total RNA extraction kit (Qiagen) according to manufacturer instructions RNA integrity determined in Bioanalyzer (Agilent's Technologies) and a total RNA-seq was performed at Eurofins genomics. DNA libraries were prepared from total RNA samples, the adaptor-ligated libraries (125bp) were enriched by PCR amplification and gel purified (average library size of ~270bp). Libraries were sequenced with the Genome Analyzer II (Illumina) using pair-end 50-bp raw fastq reads
attributes.tag: GEO Accession
attributes.value: GSM3371765