title: GSM3822226: Bull2_RNA_seq_2; Bos taurus; RNA-Seq
library_construction_protocol: Fresh semen samples were carefully laid on a monolayer of 40% Percoll. Somatic cell contamination of the sperm cells was removed by centrifugation at room temperature for 20 min at 2000 rpm. After removal of the Percoll solution, sperm pellets were washed twice in 5 mL of warm-up phosphate-buffered saline for 20 min at 2000 rpm. Total RNA extraction of sperm pellets was performed using the standard TRIzol method.  We first preamplified extracted RNA using the Smart-seq2 protocol following the manufacturer's instructions. A total of 20 ng of cDNA was subjected to library construction based on the manufacturer's instructions. Sequencing was conducted in Hiseq2500 and 250-bp paired end reads were obtained.
attributes.tag: GEO Accession
attributes.value: GSM3822226