title: RNA-seq of ventricular cardiomyocytes
design: For RNA sequencing amplified cDNA was prepared for the maximal input volume total RNA according to the manufacturers protocol (Oviation RNA-Seq System V2, NuGen). After purification SPIA-cDNA was fragmented with 25 cycles (30 sec ON/ 90 sec OFF, low energy) using a Bioruptor (Diagenode). 100 ng of fragmented cDNA was used for library preparation (NEBNext UltraTM DNA Library Prep Kit, NEB). 9-12 PCR cycles were used for library enrichment. Agencourt AMPure XP Beads (Beckman Coulter) were used for purification.