title: GSM2879534: iNPC_P25_2_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturer's instructions. The RNA was reversed transcribed by SuperScript II reverse transcriptase (Invitrogen). The double-stranded cDNA was amplified using KAPA HiFi HotStart ReadyMix (KAPA Biosystems) and purified using 1: 0.75 ratio of AMPure XP beads (Bechman Coulter). Amplified cDNA (~5 ng) was then used to construct Illumina sequencing libraries using Illumina's Nextera DNA sample preparation kit following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2879534