title: GSM4658277: 48hrAPF_Male_TRAP_5; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Translated mRNA purification was performed as described in Newell et al. 2016 with the following modifications: the 40µl of lysate supernatant (input) and TRAP (IP) fractions were placed into/resuspended in RA1 lysis buffer (Machery-Nagel Nucleospin RNA) for total RNA isolation according to manufacturer's protocol. The isolated total RNA from for TRAP and Input libraries underwent poly(A)+ selection using the NEBNext Poly(A) mRNA isolation module. TRAP and input libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.
attributes.tag: GEO Accession
attributes.value: GSM4658277