title: GSM2640579: MM1S myeloma cells grown in monoculture; Homo sapiens; RNA-Seq
library_construction_protocol: Performed as described in methods of Wiita et al eLife (2013) 2:01236.  Briefly, cells were lysed in Trizol and total RNA extracted.  Poly(A) mRNA was then isolated using New England Biolabs poly(dT) kit and quality and concentration evaluated by Nanodrop spectrophotometry. Performed as described in methods of Wiita et al eLife (2013) 2:01236.  Briefly, poly(A) mRNA was fragmented in alkaline buffer and then size-selected for 50-100 bp fragments on TBE-Urea gels.  These fragments were then ligated to a small RNA linker, reverse transcribed, circularized, and PCR amplified for final library construction.
attributes.tag: GEO Accession
attributes.value: GSM2640579