title: GSM2267223: KT_UT; Homo sapiens; RNA-Seq
library_construction_protocol: Cell culture medium was removed and cells were snap frozen.  RNA was extracted using Rneasy kit (qiagen) with on column Dnase I digestion. Intact poly(A) RNA was purified from total RNA samples (100-500 ng) with oligo(dT) magnetic beads and stranded mRNA sequencing libraries were prepared as described using the Illumina TruSeq Stranded mRNA Library Preparation Kit (RS-122-2101, RS-122-2102).  Purified libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583).  The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Individual libraries were normalized to 10 nM and equal volumes were pooled in preparation for Illumina sequence analysis.
attributes.tag: GEO Accession
attributes.value: GSM2267223