title: GSM2879467: A3_miRNA; Ovis aries; miRNA-Seq
library_construction_protocol: (mRNA) total  RNA was extracted using Trizol reagent. Ion Total RNA-seq Kit v2 (Life Technologies, USA) was used for the construction of sequencing libraries. (miRNA) miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by Gel electrophoresis. RNA libraries were prepared for sequencing using standard protocols of Total RNA-seq Kit v2 (Life Technologies, USA). Briefly, poly(A)-containing mRNA was purified by Dynabeads (Life technologies, USA) and subsequently fractionated into short fragments using RNase III and an Ion adaptor. The short RNA fragments was ligated with a 3' adaptor and a 5' adaptor.  Emulsion PCR was performed using mRNA and miRNA cDNA library as the template. Then, the short mRNA fragments were reverse-transcribed and amplified to synthetize double-stranded cDNA.
attributes.tag: GEO Accession
attributes.value: GSM2879467