title: lung microbiome from sputum for 5D_27_4.12_Recovery
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode CGAGAGATAC for subject 27 at a Recovery visit on 11_17_08