title: GSM3592650: Ferrate treatment before AF; Escherichia coli; RNA-Seq
library_construction_protocol: Cells were lysed using 5 mg/mL lysozyme in TE buffer. Following lysis, RNA was extracted using RNeasy Mini Kit (Qiagen Inc.) and following manufacturers recommended instructions. To ensure the elimination of contaminating DNA, RNA samples were also treated with DNAse 1 (Qiagen Inc.). Ribosomal RNA depletion was carried out to remove rRNA using Ribo-zero rRNA removal kit (Bacteria) from Illumina RNA libraries of purified RNA were constructed with TruSeq Stranded mRNA library preparation kit following manufacturer's instructions
attributes.tag: GEO Accession
attributes.value: GSM3592650