title: GSM2806536: Untreated_2_Input; Schizosaccharomyces pombe; OTHER
library_construction_protocol: Biological duplicates of Rpb3-flag S. pombe cells were treated or not with TSA. After lysis, each S. pombe lysate was mixed with an aliquot of a lysate of Rpb3-flag S. cerevisiae cells (spike), in a 50:1 ratio. Libraries were constructed according previously published protocol (Churchman and Weissman, 2012), with minor modifications, starting from 1L of exponentially growing cells. Ligation of DNA 3’-linker was performed as described (Marquardt et al., 2014), starting with 2-3 µg of purified nascent RNA. Ligated nascent RNA was then submitted to alkaline fragmentation for 20 minutes at 95°C. Single-end sequencing (50 nt) of the libraries was performed on a HiSeq 2500 sequencer. NET-Seq
attributes.tag: GEO Accession
attributes.value: GSM2806536