title: GSM3822246: Control3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from spinal cord tissue using QIAzol lysis reagent (Quiagen, Hilden, Germany) and purified with RNAEasy Lipid Tissue Mini kit (Quiagen). Samples were processed for high throughput sequencing using poly-A selection with the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, Illumina, San Diego, CA, USA). In brief, 1 µg of total RNA from each sample was used to construct a cDNA library, followed by sequencing on the Illumina HiSeq 2500 system with single end 50 bp reads and ~40 millions of reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM3822246