title: GSM3369540: V167M; Homo sapiens; RNA-Seq
library_construction_protocol: RNAlater-preserved samples were ground in liquid nitrogen and isolated with Trizol (Thermo Fisher Scientific) followed by column purification (EZNA Total RNA Kit I, Omega Bio-tek). 500 ng of total RNA was used as input for the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero to generate ribosomal RNA-depleted sequencing libraries. Each sample had a unique barcode to allow multiplexing, and was sequenced to >40 million raw reads in a single end 75 bp sequencing run on the Illumina NextSeq500.
attributes.tag: GEO Accession
attributes.value: GSM3369540