title: GSM1861894: NS5-untreated-RNA-seq-rep3; Mus musculus; RNA-Seq
library_construction_protocol: NSCs were harvested after 44 hrs after electroporation using 1mL of Trizol. This was mixed thoroughly with 200µl chloroform and centrifuged at max. speed for 15 minutes. The water phase was collected and subsequently the poly(A) RNA was isolated from this sample using the RNeasy kit (Qiagen). The RNA was retrieved from the column in 30 µl RNase-free water (Qiagen) RNA was isolated using the RNeasy kit (Qiagen), tested for quality with the Bioanalyzer and prepared using the TruSeq RNA sample prep kit v2
attributes.tag: GEO Accession
attributes.value: GSM1861894