title: GSM2111571: shNCAPH2_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from mouse ES cells was isolated with TRIzol Reagent (ThermoFisher Scientific, 15596) following the manufacturer’s instructions. RNA was treated with DNase I (NEB, M0303S) to remove contaminated genomic DNA. After that, RNA was depleted of ribosomal RNA with the Ribo-Zero kit (Epicentre). The ribo-depleted RNA samples were amplified with the TruSeq RNA Sample Prep Kit (Illumina) for Solexa sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2111571