title: GSM2637520: Lung single cell 1139; Mus musculus; RNA-Seq
library_construction_protocol: We used different transgenic mouse reporter lines with fluorescent expression in different populations of vascular cells, Cldn5(BAC)eGFP mice that strongly labels lung endothelial cells, mice positive for Pdgfrb(BAC)eGFP and NG2dsRed.  We chose to focus our efforts to adult mice 12 to 15 weeks old in order to primarily define fully differentiated and quiescent vascular cells and we chose a methodology based on fluorescence-activated cell sorting (FACS) into 384-well plates followed by the SmartSeq2 methodology for the generation and sequencing of full length polyadenylated cDNAs Libraries for RNA-seq were prepared by using SMART-seq2 methodology
attributes.tag: GEO Accession
attributes.value: GSM2637520