title: GSM2112671: C12-d100-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cerebral organoids by RNeasy (Qiagen), and 1 μg total RNA was primed with Oligo(dT)20 primers (Invitrogen) for cDNA synthesis. RNA-seq libraries were generated from duplicated samples per condition using the Illumina TruSeq RNA Sample Preparation Kit v2 following manufacturer’s protocol. An Agilent 2100 BioAnalyzer was used to quantify amplified cDNA and qPCR was applied to accurately quantify library concentration. 20 pM diluted libraries were used for sequencing. 50-cycle pair-end sequencings were performed using Illumina MISeq or HISeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM2112671