title: GSM2982219: IonXpress_022; Homo sapiens; RNA-Seq
library_construction_protocol: Four 5µm unstained sections underwent standard deparaffinization. All samples were enriched by manual microdissection. Total RNA was isolated using Qiagen RNeasy FFPE kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions Ten ng of total RNA  was reverse transcribed to cDNA using the Invitrogen SuperScript VILO cDNA Synthesis kit (Applied Biosystem - ThermoFisher Scientific, San Francisco, CA, USA).   Transcriptome libraries were generated according to the manufacturer's instructions. Briefly, after primers digestion, adapters and molecular barcodes were ligated to the amplicons followed by magnetic bead purification; then libraries were amplified and purified. Amplicon size and DNA concentration were measured using an Agilent high sensitivity DNA kit (Agilent Technologies, Waldbronn, Germany).
attributes.tag: GEO Accession
attributes.value: GSM2982219