title: GSM2550932: DM1_central 13; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA including low molecular weight (LMW) RNA was extracted using mirVanaTM miRNA kit (Thermo Fisher Scientific, Waltham, MA) from human central and limbal corneas.  Illumina TruSeq™ Small RNA Library Prep kit per the manufacturer’s protocol was used toconstruct libraries as recommended by manufcaturer.  In brief, adapters were ligated to both ends of the RNA, transcripts were reverse transcribed, and then cDNA was PCR amplified with indexed primers to enable multiplexing samples for sequencing.  Libraries between 145-160 base pairs long were purified from gel. Libraries were pooled in equimolar concentration for each experiment  and clustered on a single-end flow cell using an Illumina c-bot. The library-clustered flow cell was sequenced on the Illumina GAIIx, 1x36 bp sequencing chemistry.
attributes.tag: GEO Accession
attributes.value: GSM2550932