title: GSM2550463: HLF271_day21; Homo sapiens; RNA-Seq
library_construction_protocol: Freshly sorted SSEA4þ/SSEA1/small cells or their progeny (IPF and control, n Z 4; ie, 16 samples total) and were harvested and RNA was isolated using Trireagent. A minimum of 1 µg of total RNA per sample was used for the construction of sequencing libraries. Steps include oligo-dT purification of polyadenylated RNA, and reverse trascription to create cDNA. The cDNA is fragmented, blunt-ended, and ligated to indexed (barcoded) adaptors. The library was size selected (in the case of paired-end RNA-Seq), size distribution validated using capillary electrophoresis, and quantified using fluorimetry (PicoGreen) and via Q-PCR.
attributes.tag: GEO Accession
attributes.value: GSM2550463