title: GSM4601044: AB17GFP_RNA_rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNAs were isolated with TRIzol reagent (Life Technologies) and performed on three independent biological replicates. Extracted RNAs were digested with DNase I and purified using the RNeasy Mini Elute Cleanup Kit (Qiagen). The mRNAs were separated by the NEBNext Poly(A) mRNA Magnetic Isolation Module based on the coupling of Oligo d(T)25 to paramagnetic beads which was then served as the solid support for the direct binding of poly(A)+ RNA. Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit® for Illumina according to the manufacturer's instructions, and paired-end sequenced by Illumina HiSeq-2500.
attributes.tag: GEO Accession
attributes.value: GSM4601044