title: GSM4407519: WT; Mus musculus; RNA-Seq
library_construction_protocol: Tumor were harvested from 4 individual mice and pulled together to digest and process for signle cell suspension with a cocktail of Collagenase-II, Dispase-II, and Deoxyribonuclease 1. Samples were stained with PE-Cy7 CD31 and APC CD45 antibodies. CD45-CD31+ endothelial cells were FACS sorted using BD FACSAria II. Single-cell RNA-seq libraries were prepared using version 1 Chromium Single-cell 3′ Library, Gel Bead & Mutiplex Kit (10X Genomics). single-cell RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM4407519