title: GSM3135628: wild type transposon library PCP 1; Sphingobium chlorophenolicum L-1; OTHER
library_construction_protocol: Total genomic DNA was purified from cultures using the Purelink Genomic DNA Mini Kit (Invitrogen, K1820-02). Tn-Seq libraries were prepared as described in (Wiles TJ, Norton JP, Russell CW, Dalley BK, Fischer KF, Mulvey MA. 2013. Combining quantitative genetic footprinting and trait enrichment analysis to identify fitness determinants of a bacterial pathogen. PLoS Genet 9). Briefly, the transposon and the immediate surrounding genomic DNA were isolated by digestion with MmeI and size selection on an agarose gel. The fragments were then dephosphorylated, ligated to adaptors, and PCR amplified to prepare for Illumina short-read sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3135628