title: GSM2640447: Pineal Gland Night; Danio rerio; RNA-Seq
library_construction_protocol: Adult (0.5-1.5 years old) transgenic zebrafish, Tg(aanat2:EGFP), which express enhanced green fluorescent protein (EGFP) in the pineal gland under the control of the aanat2 regulatory regions, were used. Fish were raised under 12-hr light:12-hr dark (LD) cycles in a temperature controlled room. For tissue collection fish were anesthetized in 1.5 mM Tricane (Sigma), and sacrificed by decapitation. Dissections were conducted at mid-day (ZT6) and mid-night (ZT18). Fluorescent pineal glands, whole eyes and other tissues were removed under a dissecting microscope. A pool of 18 pineal glands 6 eyes and 4-8 of each peripheral tissue was collected at each time point. Total RNA for mRNA analysis was isolated using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer's instructions. The mixed tissue samples consisted of equal amounts of total RNA (1mg) from muscle, ovary, kidney, gill, liver, intestines, heart and brain. Poly-A RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2640447