title: GSM2107408: shUSP51_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from sub confluent (~75%) MCF7 cells expressing non-targeting shRNA or shRNA targeting either USP22, USP27x or USP51 was isolated using an RNeasy kit (Qiagen cat# 74134). The purified RNA was then treated with TUBRO DNase. (Ambion cat. # A1907) and used for library preparation. RNA-Seq sequencing libraries were made using TruSeq RNA library preparation kit (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM2107408