title: CsK2_mtDNA
design: High quality DNA was isolated from a pool of adults of C. sinensis using the Circulomics Tissue Kit (Circulomics, Baltimore, MD). Subsequently, low molecular weight DNA was removed using a 5 or 20 kb short read eliminator (SRE) kit (Circulomics, Baltimore, MD). High molecular weight C. sinensis genomic DNA was used to construct a Nanopore rapid barcoding (SQK-RBK004: Oxford Nanopore Technologies; 5 kb SRE) and two Nanopore ligation sequencing (SQK-LSK109: Oxford Nanopore Technologies; 5 and 20 kb SRE) gDNA libraries according to the manufacturers instructions. The rapid barcoding (SQK-RBK004; 5 kb SRE) and a ligation sequencing (SQK-LSK109; 5 kb SRE) library were sequenced on separate flow cells (version 9.4.1; Oxford Technologies). The flow cell used to sequence the SQK-LSK109; 5 kb SRE library was washed using a Flow Cell Wash Kit (EXP-WSH003; Oxford Technologies) according to manufacturers instructions and re-used to sequence the SQK-LSK109; 20 kb SRE library. All genomic libraries were sequenced on the MinION sequencer (Oxford Nanopore Technologies). Following sequencing, bases were called from raw fast5 reads using the program Guppy v.3.1.5 (Oxford, Nanopore) and stored in the FASTQ format