title: RNA-Seq of Jacobaea aquatica: pooled shoots and roots of 7 individuals
design: Total RNA was extracted with the NucleoSpin RNA Plant-Macherey-Nagel kit, mRNA was selected using oligo-dT Dynabeads. Then, RNA were fragmented to generate double stranded cDNA followed by duplex-specific thermostable nuclease treatment to remove abundant library molecules. Sequencing was performed on a HiSeq2500 platform.