title: GSM2879356: FS1; Homo sapiens; RNA-Seq
library_construction_protocol: Passage 3 or 4 organoid samples (n = 3; 200 ul of BME per sample) and matched cortical kidney tissue samples (n = 3) were harvested with TRIzol reagent (Life Technologies) and RNA was isolated according to manufacturer's instructions. RNA samples were processed into cDNA libraries based on the CEL-seq2 technique as described previously. Illumina sequencing libraries were prepared with the TruSeq small RNA primers (Illumina) and sequenced paired-end at 75 bp read length the Illumina NextSeq. Tissue samples and organoids were processed and sequenced in parallel.
attributes.tag: GEO Accession
attributes.value: GSM2879356