title: lung microbiome from sputum for 53B_77_3.1_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode TCACGTACTA for subject 77 at a Exacerbation visit on 03_16_09