title: GSM3592384: Yamato-SS RNA-seq [YAMA_XO5]; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using qiagen RNeasy kit along with Dnase treatment for 30 minutes at RT. 500 ng of total RNA was subjected to library synthesis using theNEB Ultra II RNA Library kit.NEBNext® Poly(A) mRNA Magnetic Isolation Module was used to enriched for the Poly-A mRNA . Illumina indexes prior to 12 cycles of PCR. Sequencing was performed using 12.5 pM hybridization to a HiSeq V4 2x50 paired end flow cell.
attributes.tag: GEO Accession
attributes.value: GSM3592384