title: GSM4106224: Aged USC rep 3 [lane5784_GATGAATC_A43_0d]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Allprep DNA/RNA Mini kit (Qiagen 80204) followed by Dnase treatment using the TURBO DNA-free kit (Life Technologies AM1907) mRNA was isolated from total DNA-free RNA (350ng) using the Dynabeads mRNA purification kit (Life Technologies 61006). Indexed, strand specific libraries were prepared using the Agilent SureSelect Strand specific Library Preparation kit (Agilent, G9691A) according to the manufacturer's instructions. Libraries were quantified/assessed using both the KAPA Library Quantification Kit (KAPA Biosystems KK4824) and Bioanalyzer 2100 system (Agilent). Indexed libraries were pooled and sequenced with a 100bp single-end protocol on an Illumina HiSeq2500 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM4106224