title: GSM4041772: Patient7; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from each ovarian cancer tissue sample using an RNeasy Plus Kit (Qiagen), with QIAshredder spin columns for homogenization and on-column DNaseI digestion (79254, Qiagen).  The total RNA was analyzed for integrity using an Agilent 2100 Bioanalyzer.  The RNA collected was then processed for whole-genome polyadenylated RNA sequencing (polyA+ RNA-seq). The total RNA samples were enriched for polyA+ RNA using Dynabead Oligo(dT)25 (Invitrogen) and strand-specific RNA-seq libraries were prepared from the polyA+ RNA as described previously.  The RNA-seq libraries were sequenced using an Illumina HiSeq 2500 (single-end, 50 nt; SE50; one replicate).
attributes.tag: GEO Accession
attributes.value: GSM4041772