title: GSM3731874: NV_GB_M1_1; Nezara viridula; RNA-Seq
library_construction_protocol: Tissue were dissected in PBS 0.1M pH 7.4, and then collected in RNA-later. For processing, all RNA-later was removed and total RNA was extracted through Ambion PureLink RNA extraction kit according to manufacturer's instructions. Eluted RNA was treated with DNAse I for 30 min. Reactions were cleaned up by overnight precipitation at -20 C with isopropanol and sodium acetate pH 3.2. After centrifugation, pellet was washed with 70% Ethanol in Rnase free water, and final pellet resuspended in Rnase free water. Sequencing facility prepared mRNA libraries by standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3731874