title: GSM3369721: 1.WT CD4+ CD90.1+ T cells (replicate 1); Mus musculus; RNA-Seq
library_construction_protocol: Cells enriched by flow cytometry were directly collected in RNA extraction buffer Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 10 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA assay.
attributes.tag: GEO Accession
attributes.value: GSM3369721