title: Amplicon sequencing of CRISPR/Cas9 genome edits at imprinted CpG islands
design: 25 cycles of pCR using locus specific primers with 5' extensions containing a random hexamer, illumina adapters and binding sites for universal for a second round of PCR. Primers for the second round (8 cycles) contained 5 ' extensions with unique indices (Nextera i5 and i7).