title: GSM2982230: IonXpressRNA002; Acinetobacter baumannii; RNA-Seq
library_construction_protocol: LB broth, Dnase-treated RNA (Ab-2_clon_2010 untreated versus Ab-2_clon_2010CHLX treated with CLHX) was isolated from stationary-phase cultures (OD=1.0-1.5) with the High Pure RNA Isolation Kit (Roche, Germany). The samples were treated with the Ribo-Zero rRNA Removal kit (Illumina, San Diego, California) in order to eliminate the ribosomal RNA (23S, 16S and 5S). The quality of the RNA samples was examined in a 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, California). Transcriptome libraries were constructed using the Ion Total RNA-Seq Kit v2 and Ion XpressTM RNa-Seq Barcode 01-16 kit (Thermo Fisher Scientific, Waltham Massachusetts) according to the manufacturer's instructions. The libraries were quantified in the 2100 Bioanalyzer system. The mix reaction was carried out in automated Ion Chef systems (Thermo Fisher Scientific, Waltham Massachusetts): Ion PITM Hi-QTM Chef and Ion PITM chips v3 kits (Thermo Fisher Scientific, Waltham Massachusetts). The resulting readings (all >80 million) were used to generate FASTQ files with the FileExporter 4.6.0.0 plugin (19).
attributes.tag: GEO Accession
attributes.value: GSM2982230