title: GSM2724023: NAT10_RNA-Seq_Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified from cultured cells using Trizol (ThermoFisher) followed by treatment with TurboTM DNase (ThermoFisher). Enrichment of polyadenylated RNA (poly(A)-RNA) from DNase-treated total RNA was carried out by two rounds of selection with Oligo-(dT)25 Dynabeads (ThermoFisher) according to the manufacturer’s instructions. Enrichment efficiencies were estimated with Bioanalyzer picoRNA chips (Agilent Technologies, Santa Clara, CA). Total RNA was used for library construction with the Illumina TruSeq stranded RNA Protocol RS-122-2201 protocol (Illumina, San Diego, CA), including rRNA depletion via RiboZero
attributes.tag: GEO Accession
attributes.value: GSM2724023