title: GSM3137315: tgmel.58_WF_trametinib-20nM; Oryzias latipes; RNA-Seq
library_construction_protocol: Fish samples were homogenized in TRI reagent followed by addition of 200 μl/ml chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15 min at 4 °C. Total RNA was furhter purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by incubating RNA samples with DNase for DNA digestion at 25°C for 15 min.Totall RNA concentration was determined using a Qubit 2.0 fluorometer (Life Technologies, Grand Island, NY, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) to confirm that RIN scores were above 8.0 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3137315