title: 16S rRNA amplicon sequencing: mock sample 2
design: Construction of the 16S rRNA  amplicon library was based on the dual-index strategy, multiplexing 8 forward  and 12 reverse primers. PCR amplification primers targeting the V3-V4  variable regions of the 16S rRNA gene were designed to contain a 58-bp adapter  sequence specific for the Illumina MiSeq platform at the 5, a 3 to 6-bp  variability region, a 12-bp Golay barcode, and a 16S rRNA-gene specific  primer sequence universal for Prokaryotes.