title: lung microbiome from sputum for 33A_171_3_Stable
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode TCTCTATGCG for subject 171 at a Stable visit on 02_03_10