title: GSM2640299: AMC6_RNAseq; Rattus norvegicus; RNA-Seq
library_construction_protocol: Extracted and purified using the Qiagen RNeasy kit Total RNA was isolated from AMC and control hypothalamus using the RNeasy Kit (Qiagen, Valencia, CA). Total RNA samples were then processed with a Gene Read ribosomal RNA depletion kit (Qiagen, Germantown PA) prior to Illumina Hi-Seq library preparation using a directional RNA-seq library kit (NEB, Ipswich, MA). Libraries were checked by Bioanalyzer analysis (Agilent, Santa Clara, CA) for quality, then 50 base pair, single end reads were acquired on a Hi-Seq 2500 (Illumina, San Diego, CA) at the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkley.
attributes.tag: GEO Accession
attributes.value: GSM2640299