title: Illumina MiSeq Sequencing of V3-V4 16S rRNA Amplicons of G. mellonella gut: 1day, bioreplicate1
design: Three healthy G. mellonella larvae were freeze killed and left at room temperature.  One day after killing, DNA extraction was done via methodology recommended by the manufacturer of the PowerSoil kit (MoBio Laboratories, CA, USA). Extracted DNA was quantified with the Quant-iT PicoGreen Assay kit (Life Technologies, NY, USA) and used for V3/V4 16S rRNA locus amplification with custom Illumina barcoded primers based on 341F and 806R sequences.  A paired-end MiSeq run was performed on a 2x250 v2 MiSeq cartridge; the run was 251 cycles (forward) x 12 cycles (index/barcode) and 251 cycles (reverse) using components and methodology according to the manufacturer�s instructions.