title: GSM3731585: EBs_Control_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from mESCs and EBs using the RNeasy mini kit (Qiagen) with on-column DNase I digest following the manufacturer's protocol, quantified with a Thermo NanoDrop and quality tested on Agilent Bioanalyzer. NGS libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina) according to the manufacturer's recommendations and amplified in 12 PCR cycles. Libraries were profiled in a High Sensitivity DNA chip on a 2100 Bioanalyzer and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life Technologies). The NGS libraries were sequenced on a HiSeq 2000 Illumina sequencer, for 51 cycles plus seven cycles for the index read.
attributes.tag: GEO Accession
attributes.value: GSM3731585