title: GSM2981757: LAN-6-input-3496_S45; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Immune complexes were then washed three times with RIPA buffer, once with RIPA-500 (RIPA with 500 mM NaCl), and once with LiCl wash buffer (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 250 mM LiCl, 0.5% NP-40, 0.5% deoxycholic acid). Elution and reverse-crosslinking were performed in direct elution buffer (10 mM Tris-Cl [pH 8.0], 5 mM EDTA, 300 mM NaCl, 0.5% SDS) with proteinase K (20 mg/ml) at 65°C overnight. Eluted DNA was purified using AMPure beads (Beckman-Coulter). Libraries were prepared using NEBNext Ultra DNA Library kit (E7370).
attributes.tag: GEO Accession
attributes.value: GSM2981757