title: GSM2806116: high-four; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from sorted Nestin-GFPbright AECs, Nestin-GFPdim AECs and SECs was extracted using the RNAeasy Plus Micro kit (Qiagen). The integrity and purity of total RNA were assessed using an Aligent 2100 Bioanalyzer (Agilent Technologies). Complementary DNA was generated using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories) from total RNA. The Nextera XT DNA Sample Preparation Kit (Illumina) was used for the preparation of DNA libraries. The libraries were then submitted for Illumina HiSeq2500 sequencing (Illumina) according to standard operation procedures for RNAseq.
attributes.tag: GEO Accession
attributes.value: GSM2806116