title: GSM3212814: A549/cDDP_CTRci2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were collected 48 hours post-transfection (24h post-cisplatin treatment) and RNA extraction was carried out using ReliaPrep RNA Cell Miniprep System The mRNA is enriched using oligo(dT) beads and fragmented randomly in fragmentation buffer, followed by cDNA synthesis using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) is added with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation. The final cDNA library is ready after a round of purification, terminal repair, A-tailing, ligation of sequencing adapters, size selection and PCR enrichment.
attributes.tag: GEO Accession
attributes.value: GSM3212814