title: RNA-Seq of motor neurons; ALS
design: iPSC were differentiated into spinal MN using a protocol adapted from Maury et.al. Nat. Biotech. 2015. Overall efficiency of MN generation was 70-75%.Total RNA was extracted using the Qiagen miRNeasy kit.Total RNA was ribosome depleted using the NEB ribosomal depletion kit that uses oligo hybridization followed by RNase digestion. RNA sequencing libraries from the ribosome-depleted RNA were prepared using the NEBNext Ultra RNA library prep kit for Illumina and sequenced on the HiSeq4000.