title: GSM2639667: HCT-116 cells untransfected; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested by washing with Dulbecco’s PBS, addition of 500 μL Trizol (Life Technologies catalog # 15596-026), and scraping.  50 μg glycogen (RNase-free) was mixed into each sample.  A standard Trizol isolation protocol was then performed, with final resuspension in nuclease-free water. Three single-read libraries were prepared using the TruSeq RNA Library Preparation Kit (Illumina catalog # RS-122-2001) according to Illumina's instructions.  Agilent Bioanlyzer was used to quantify libraries, and the OSU Genomics Shared Resource performed next-generation sequencing on an Illumina HiSeq instrument.
attributes.tag: GEO Accession
attributes.value: GSM2639667