title: GSM4600769: CD8WT2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from FACS-sorted CD8+ T cells and snap frozen colons using the RNeasy Micro Kit (Qiagen). RNA samples were analyzed on a 2100 Bioanalyzer using Agilent RNA 6000 Pico chip (both from Agilent Technologies, Santa Clara, USA). Library preparation for isolated CD8+ T cells was performed using the SMARTer® Stranded Total RNA Sample Prep Kit –HI (Takara Bio Europe, Saint-Germain-en-Laye, France). Quantity and quality of the cDNA libraries were determined by Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, Dreieich, Germany) and Agilent High Sensitivity DNA chip (Agilent Technologies). Library preparation of total colons was performed by the DKFZ Genomics & Proteomics Core Facility.
attributes.tag: GEO Accession
attributes.value: GSM4600769