title: GSM2266359: 72h incubation in the absence of As(III) R2; Thiomonas sp. CB2; RNA-Seq
library_construction_protocol: enriched mRNA was obtained from total RNA using the rRNA capture hybridization approach from the RiboZero kit Supernatant was gently removed. 1.5 mL of physiological water at 4°C was added. biofilms were scraped on ice using a cell scraper (Biologix), centrifuged at 8000 x g for 15 min at 4°C. Cell lysis was obtained by shaking the tubes using a Retsch disruptor during three 30-second cycles and centrifuging them at 16 000 x g for 5 minutes at 4°C. The aqueous phases were then transferred to fresh tubes and treated with Trizol Directional libraries were prepared using the TruSeq Stranded total RNA Sample preparation kit following the manufacturer’s instructions (Illumina). The libraries were quantified by fluorimetric measurements with the Qubit® dsDNA HS Assay Kit (ThermoFisher). 51-bp Single Read sequences were generated on the Hiseq2000 sequencer according to manufacturer’s instructions (Illumina). Sequences were demultiplexed using the Illumina pipeline (Gerald, included in CASAVA version 1.7) giving FASTQ formatted reads.
attributes.tag: GEO Accession
attributes.value: GSM2266359