title: Illumina HiSeq 2500 sequencing; Phosphoglycerate dehydrogenase causes electron transport chain defect via impaired heme synthesis: RNA sequencing.
design: Phosphoglycerate dehydrogenase causes electron transport chain defect via impaired heme synthesis: RNA sequencing.
library_construction_protocol: Ctrl and PHGDHKD human ECs were grown in 10cm dishes and 4 days after transduction, RNA was collected to perform RNAsequencing RNA libraries were created using the KAPA mRNA stranded library prep kit according to the manufacturer's instructions.
attributes.tag: Experimental Factor: Donor968
attributes.value: individual
attributes.tag: Experimental Factor: PHGDH Knock Down
attributes.value: genetic modification