title: GSM2805921: cont_f0_12; Danio rerio; RNA-Seq
library_construction_protocol: Livers were briefly rinsed in 1x PBS, snap frozen with liquid nitrogen and stored at -80°C for transcriptome analysis (RNA-sequencing). Total RNA was extracted using QIAzol Lysis Reagent (Qiagen) and RNA samples were DNase treated with the DNA-freeTM DNA Removal Kit (Ambion) in order to avoid remaining genomic DNA. RNA quantity was verified using NanoDrop® ND-1000 Spectrophotometer (NanoDrop Technologies, USA). RNA integrity (RIN) was determined using an Agilent 2100 Bioanalyser (RNA 6000 Nano LabChip kit, Agilent Technologies, USA). RIN values from the 12 extracted RNA samples were on average 9.06 ± 0.39 The Norwegian Sequencing Centre (CEES) performed RNA-sequencing (RNA-seq) and library preparation using TruSeqTM Stranded mRNA Library Prep Kit (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM2805921