title: GSM3673514: MIA-PaCa-2_BI00923406_50mgkg_10h_rep1_J19537; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in TRI lysis reagent (Qiagen, #79306) according to the manufacturer's instructions. Instead of chloroform, 10% volume 1-bromo-2-chloropropane (SigmaAldrich, # B9673) was added. Total RNA was isolated with RNAeasy Mini Kit (Qiagen, #73404). Quant-seq libraries were prepares using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina from Lexogene (# 015.96) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3673514