title: GSM4261163: H3K9me3 40%-30-1; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-Seq cells were fixed in 1% methanol-free paraformaldehyde after which cells were scraped in PBS, lysed, and sonicated using a Covaris M20 sonicator to generate 300-600 bp fragments. 3 µg of antibody (H3K9me3 Abcam ab8898 or IgG control Cell Signaling #5415) and protein G Dynabeads were added to precipitate solubilized chromatin. Samples were subsequently eluted and crosslinks were reversed. DNA treated with proteinase K and RNAse was purified by phenol:chloroform extraction, followed by ethanol precipitation, after which DNA content was quantified using Qubit. For ChIP sequencing with TruSeq ChIP library preparation kit according to the manufacturers protocol.
attributes.tag: GEO Accession
attributes.value: GSM4261163