title: GSM4260264: EdU-seq-WT rep3; Homo sapiens; OTHER
library_construction_protocol: Sequencing libraries were prepared using NEBNext Ultra DNA library preparation kit.  In brief, the DNA was end-repaired, adaptor-ligated, and PCR-amplified. EdU-seq:  Cells were labeled with 10 µM EdU for 3 hr.  Cells were fixed with 70% ice-cold ethanol, washed, and incubated in click-reaction buffer containing 100 µM biotin-dPEG11-azide (Quanta Biodesign), 2 mM CuSO4, and 10 mM sodium ascorbate in TBS for 15 min.  Genomic DNA was isolated and sonicated to 300-500 bp with a Branson 450 sonifier.  Biotinylated DNA was collected on 20 µl of Dynabeads MyOne Streptavidin T1 magnetic beads.
attributes.tag: GEO Accession
attributes.value: GSM4260264