title: Single Cell Whole Genome Seq: adult GBM: Sample HM001_GBM_1_R_9_S57
design: Frozen glioma samples were mechanically dissociated into pools of single nuclei as previously described following which single nuclei were isolated by flow cytometry, using a DAPI-based stain. Nuclei were subjected to whole-genome multiple displacement (MDA) amplification (Qiagen  REPLI-g) followed by next-generation sequencing library construction for Illumina Sequencing (Qiagen QIAseq FX DNA library kit). Libraries were sequenced on the Illumina HiSeq platform in paired end mode. Single cells were sequenced to 0.1  1X coverage. Bulk pooled nuclei were sequenced to 60X coverage while matched germline DNA (extracted from blood) was sequenced to 30X coverage.
attributes.tag: alignment_software
attributes.value: bwa mem