title: lung microbiome from sputum for 81A_19_2.3_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ATACGACGTA for subject 19 at a Exacerbation visit on 07_14_08