title: GSM3464057: Atl2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from activated CD4+ T cells using the RNeasy mini kit (Qiagen). For the RNAseq library preparation, mRNA was purified from total RNA with oligo-dT beads and sheared by incubation at 94 degrees.  Following first-strand synthesis with random primers, second strand synthesis was performed with dUTP for generating strand-specific sequencing libraries. The cDNA library was then end-repaired, and A-tailed, adapters ligated, and second-strand digestion was performed by U-DNA-Glycosylase. Indexed libraries that meet appropriate cut-offs were quantified by qRT-PCR and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM3464057