title: GSM2879057: KO_ABX_Jejunum_MM317-25; Mus musculus; RNA-Seq
library_construction_protocol: Small and large intestine were removed and transferred into cold PBS. A piece (~ 5mm) of whole intestinal tissue (duodenum, jejunum, ileum and colon) was soaked in RNAlaterTM (Qiagen) at 4 ºC for 48 hours and then stored at -80 ºC until further analysis. For RNA extraction a Tissue-Tearor Homogenizer (Biospec) was used. RNA was prepared using the RNeasy Mini Kit (Qiagen). RNA libraries were prepared using the TruSeq® Stranded Total RNA with Ribo-ZeroTM Gold kit (Illumina) and sequenced using 50- and 100-base pair single-end reading on a HiSeq 2500 and 4000 instruments, respectively (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2879057