title: GSM2881083: RNA-seq_jizi; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: Harvested samples were either frozen in liquid nitrogen for re-sequencing and RNA sequencing. or directly vacuum-infiltrated with formaldehyde cross-linking solution for ChIP assay. ChIP assay was performed with the antibodies against H3 trimethyl-Lys 27 (Upstate, USA, Cat. 07–449) and H3 trimethyl-Lys 4 (Abcam, Cat. ab8580) for wheat. For rice samples, ChIP assay was performed with the antibody against H3 trimethyl-Lys 4 (Abcam, Cat. ab8580), H3 monomethyl-Lys 4 (Millipore,07-436), H3 trimethyl-Lys 27 (Upstate, USA, Cat. 07–449), H3 acetyl-Lys 27 (Upstate, USA, Cat. 07–360) and H3 trimethyl-Lys 36 (Abcam, Cat. ab9050) Library construction and deep sequencing were performed by Genergy Biotechnology Co. Ltd. (Shanghai, China). The DSN kit from Illumina was used for DSN library normalization. Libraries were sequenced on the HiSeq2000/ 2500 (Illumina) to produce 50 bp single reads for rice, and 150 bp paired reads for wheat.
attributes.tag: GEO Accession
attributes.value: GSM2881083