title: Illumina MiSeq paired end sequencing
design: Endotrachael aspirates from trauma patients at ICU admission (n = 74) and 48 hours (n = 30) were sequenced to characterize bacterial communities and determine whether cigarette smoking contributes to ARDS development via alteration of the lung microbiota
library_construction_protocol: DNA was extracted using lysing matrix E tubes and AllPrep kit (Qiagen) protocol; V4 region of 16S rRNA amplified with 515F and 806R; sequenced on Illumina MiSeq