title: GSM2550542: Mouse_dermis_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Whole tissue was homogenized using Precellys in RLT buffer supplemented with beta-mercapto ethanol to preserve RNA integrity. Total RNA was isolated using Rneasy micro kit as per manufacturer's suggestions (Qiagen), including DNase treatment. Total RNA was eluted in molecular grade water. RNAs with optimal RIN scores were considered for library preparation. SMART-seq2 was performed on total RNA as described by Picelli et al. Briefly, 10 ng and 100 ng total RNA was used for RT. Resulting cDNA was pre-amplified for 12 and 10 cycles, respectively and tagmentation  performed on 18 ng and 20 ng cDNA using the Nextera DNA Sample Preparation Kit (Illumina). Fragments were amplified for 8 continuous cycles. Libraries sequenced as single-end (86bp and 43bp) on a NextSeq 500 Illumina Sequencing Platform (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2550542