title: RNA-Seq of Gallus gallus: 8 week male broiler liver; High lysine rep2
design: Liver mRNA profiles of 8-week-old high lysine and low lysine of broiler chicken were generated by deep sequencing, in triplicate, using the Ion PGM System. Bam files were converted to Fastq files for further analysis. The quality of the raw data in fastq format was assessed. Low-quality reads containing sequencing adaptors and poly-N, unknown bases, and low-quality bases were removed. Validation of sequencing data was done by quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed SYBR Green assays.