title: A2 FLX cDNA - accession 8401
design: RNA was provided to Washington University in St. Louis to develop 454 EST sequences on the FLX platform.  cDNA was poly-T primed and amplification adaptors included modified SMRT sequences that allowed for trimming of the poly-A (or -T) sequences prior to sequencing based on an MmeI cut site.  Once the cDNA was SMRT amplified, the library was normalized using Evrogen's Trimmer kit (DSN).  A sample (0.5 FLX plate) was sequenced using FLX technology (prior to Titanium) and the raw reads contain some adaptor sequences.  The bead library was then sent to BYU on dry-ice were 2 additional 0.5 plates were sequenced from the same 454 bead library (same titer was used). The raw 454 reads contain adaptor sequences. These reads are in the following files: FEV57AF01.sff,  FI1UJHG02.sff, and  FI1UJHG01.sff