title: GSM3214219: cdk1-as1 S phase; Saccharomyces cerevisiae; ncRNA-Seq
library_construction_protocol: Cells were synchronized by culturing in YPD to log phase, after which 15 mg/L α factor was added for 3 hrs. Synchronized cells were released into the cell cycle by washing away the pheromone and resuspending the cells in YPD or in YPD + nocodazole (10 mg/L) to prevent initiation of a new cell cycle. Cells were harvested after release from pheromone arrest when cells reached S-phase based on microscopy.Total RNA was isolated by using  QIAGEN kit. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3214219