title: GSM4105969: mNET-seq Mouse HS2HS3 fetal liver cells late erythroid (24h) replicate 1; Mus musculus; OTHER
library_construction_protocol: isolation protocol5x10^6 cells were aliquoted per replicate, pelleted by centrifugation (500g, 5min, R.T) and washed once in ice cold 5mL 1xPBS before snap freezing in a dry ice ethanol bath. Samples were snap frozen and transferred to storage at -80°C until use.  The antibody used for the mNET-seq samples is no longer commercially available. Antibody used in this study was kindly provided by Dr. Hiroshi Kimura. The antibody targets Pol II total CTD. mNET-seq was performed as in (Nojima et al. 2015) but with a starting input cell number of 5x10^6 NEB small RNA for Illumina
attributes.tag: GEO Accession
attributes.value: GSM4105969