title: GSM2205567: P60_28; Bos taurus; miRNA-Seq
library_construction_protocol: RNA was extracted from 0.7 mL of plasma using TRIzol LS (Life Technologies, USA), following the manufacturer’s protocol. During the RNA extraction protocol, glycogen (Sigma-Aldrich, USA) was added to each sample to facilitate the precipitation of RNA, and an exogenous miRNA control, syn-cel-miR-39-3p (Qiagen, NL), was spiked into each sample. RNA was re-suspended in 20 μL of RNase-free water and used immediately or frozen at -80 °C. Small-RNA libraries were prepared using the Illumina TruSeq small-RNA sample preparation kit (Illumina, USA) following the manufacturer’s protocol. Libraries were submitted to 36-base single-end sequencing using the Illumina HiSeq 2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM2205567