title: Illumina HiSeq 1500 sequencing of SAMD00089599
library_construction_protocol: R. irregularis DAOM181602 was axenically cultured on modified M medium with 100 ????????????????????????M palmitoleic acid at 28????????????????C for 42 days. 20 germ tubes were dissected from spores and sampled. RNA was extracted by using NucleoSpin RNA XS kit (MACGEREY-NAGEL) with RNase-Free DNase Set (Qiagen). RNA was reverse-transcribed and amplified by using SMART-Seq v4 kit. cDNA quality and quantity were checked by using Agilent 2100 Bioanalyzer. cDNA was sheared by using Coveris S2 system and transformed to illumina libraries by using Low imput library prep kit (Chlontec). After the quality and quantity were confirmed by using Agilent 2100 Bioanalyzer and KAPA Library quantification kit, the libraries were sequenced by HiSeq 1500.