title: GSM4157939: shield Flag-Elavl1a iCLIP rep2; Danio rerio; OTHER
library_construction_protocol: iCLIP-seq was carried out as previously described (Despic et al., 2017). Breifly, flag-elavl1a mRNA injected embryos were collected at 4 h.p.f. and 6 h.p.f.. 400 zebrafish embryos were irradiated twice with 0.8 J/cm2 (Stratalinker 2400, Stratagene), lysed, and subjected to mild RNA fragmentation. Crosslinked RNA-proteins complexes were immunopurified using Anti-FLAG M2 Magnetic Beads (Merck, M8823) for 4 h.p.f. and 6 h.p.f. at 4 °C. RNA was extracted Library construction was performed by using Smarter smRNA-Seq kit (Clontech Laboratories Inc)
attributes.tag: GEO Accession
attributes.value: GSM4157939