title: GSM3213999: CD11b(AdTGFb1, Exp3) (DC 955); Mus musculus; RNA-Seq
library_construction_protocol: RNeasy Micro-Kit from Qiagen 500 ng of total RNA per sample were utilized as input for mRNA enrichment procedure with 'NEBNext® Poly(A) mRNA Magnetic Isolation Module' (E7490L; New England Biolabs) followed by stranded cDNA library generation using 'NEBNext® Ultra Directional RNA Library Prep Kit for Illumina' (E7420L; New England Biolabs). All steps were performed as recommended in user manualE7420 (Version 6.0_08-2015; NEB) except that all reactions were downscaled to 2/3 of initial volumes. Furthermore, one additional purification step was introduced at the end of the standard procedure, using of 1x 'Agencourt® AMPure® XP Beads' (#A63881; Beckman Coulter, Inc.). cDNA libraries were barcoded by single indexing approach, using 'NEBNext Multiplex Oligos for Illumina – Set 1' (Index Primer 1 – 12). All generated cDNA libraries were amplified by 10 cycles of final pcr.
attributes.tag: GEO Accession
attributes.value: GSM3213999