title: DNASeq of MDR E. coli from Cuba
design: For the long reads, Nanopore sequencing technology was performed. Sequencing library was prepared using the 1D ligation barcoding kit (catalog numbers SQK-LSK108 and EXP-NBD103; ONT, Oxford, United Kingdom) in an R9.4 SpotON flow cell (catalog number FLO-MIN106) with a MinION Mk1B device (ONT, Oxford, United Kingdom). The reads were base-called and demultiplexed using Guppy basecaller (v2.3.7) and Guppy barcoder, respectively with default settings (Oxford Nanopore Technology, Oxford, United Kingdom).