title: GSM4314092: RTK17.1_RNAseq C818; Methylacidiphilum sp. RTK17.1; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Mo Bio PowerBiofilm RNA Isolation kit following the manufacturers recommended protocol, eluted into 100 ml ddH2O and quantified using the Qubit HS assay kit (Thermo Fisher Scientific). Following this, 2mg RNA was transferred into RNAstable preservation medium (Sigma Aldrich) and dehydrated at room temperature before transport for transcriptome sequencing (Custom Science; China). Upon receipt of samples, RNA integrity was confirmed using an Agilent 2100 Bioanalyser. Following total RNA extraction, ribosomal RNAs were removed using the Ribo-Zero rRNA removal kit (bacteria) and the quality of the remaining RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent). Library construction was performed using the TruSeq Stranded Total RNA Sample Prep (microbe) Kit (Illumina) and sequencing was performed using an Illumina HiSeq2500 platform. Ribo-Zero User Guide, TruSeq Stranded Total RNA prep
attributes.tag: GEO Accession
attributes.value: GSM4314092