title: GSM4661144: RNA_CH22_PARENTAL_THZ1_24H_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA-seq: RNA extraction was performed using the RNA Clean and Concentrator-5 kit (Zymo R1017). ERCC RNA Spike-In Control Mixes (ThermoFisher 4456740) were added to each sample. SLAM-seq: RNA was extracted as described in Herzog et al., 2017. ERCC RNA Spike-In Control Mixes (ThermoFisher 4456740) were added to each sample. Total RNA-seq: Libraries were constructed using the NEB Ultra II Directional Library Prep Kit for Illumina (NEB E7760L). SLAM-seq: Libraries were constructed using the Quant-seq 3'-end mRNA library preparation kit for Illumina (Lexogen 015) Total RNA-seq: NEB Ultra II Directional Library Prep Kit for Illumina (NEB E7760L). SLAM-seq: Libraries were constructed using the Quant-seq 3'-end mRNA library preparation kit for Illumina (Lexogen 015).
attributes.tag: GEO Accession
attributes.value: GSM4661144