title: GSM2723926: 40911432T ccRCC RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Qiagen RNeasy Mini kit. RNAseq libraries were prepared using Illumina Tru-Seq RNA Sample Preparation v2 protocol, according to the manufacturer’s instructions. Briefly, poly-A RNAs were recovered from 1 µg of input total RNA using poly-T oligo conjugated magnetic beads. The recovered poly-A RNA was chemically fragmented and converted to SuperScript II and random primers. The second strand was synthesized using the Second Strand Master Mix. Libraries were validated with an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA), diluted to 11 pM and applied to an Illumina flow cell using the Illumina Cluster Station.
attributes.tag: GEO Accession
attributes.value: GSM2723926