title: Zea mays, VIT_B73_AJ_02, 14 day seedling,  Oxford Nanopore PCR-cDNA Sequencing Kit, SQK-PCS109
design: Oxford Nanopore PCR-cDNA Sequencing Kit,  SQK-PCS109, Guppy v3.2.4 --config dna_r9.4.1_450bps_hac.cfg, --trim_strategy  dna,--min_qscore 7