title: GSM2549503: UTX_ChIPseq; Homo sapiens; ChIP-Seq
library_construction_protocol: MCF-7 cells were maintained in DMEM supplemented with 10% fetal bovine serum. Approximately 5 x 107 cells were used for each ChIP-seq assay. The chromatin DNA precipitated by polyclonal antibodies against UTX. The DNA was purified with the Qiagen PCR purification kit. The 1st strand cDNA was synthesized with random primer flanked by 7 nt sequence to track infromation. The 2nd strand DNA was generated with N6 adaptor. The resulting DNA libraries were amplified with Phusion High-Fidelity DNA Polymerase.
attributes.tag: GEO Accession
attributes.value: GSM2549503