title: lung microbiome from sputum for E08_76_4.2_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ACGCTCGACA for subject 76 at a Exacerbation visit on 12_15_08