title: Modulation of sub-acute ruminal acidosis by active-dry yeast supplementation and its effect on rumen fungal and protozoal populations in liquid, solid, and epimural fractions.
design: Protozoa were amplified using previous protocols (Ishaq et al., 2015; Ishaq and Wright, 2014) that utilized the primers P-SSU-316F (5ê-GCTTTCGWTGGTAGTGTATT-3ê) (Sylvester et al., 2004) and GIC758R (5ê- CAACTGTCTCTATKAAYCG -3ê) (Ishaq and Wright, 2014) which target the V3 - V4 region of the 18S rRNA gene and signature regions 3 - 4.