title: GSM3212633: S2; Homo sapiens; RNA-Seq
library_construction_protocol: The different NK cell populations were FACS-purified on an ARIAIII cell sorter (Becton Dickinson (BD), Franklin Lakes, NJ, US). The gating strategy is depicted in Fig. 1A. The NK cell populations were collected in NucleoSpin RA1 lysis buffer (Machery Nagel, Düren, DE) and stored at -80oC prior to analyses. The total numbers of cells analyzed for each purified NK cell population are provided in Table S4. Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey Nagel), according to the instructions of the manufacturer. RNA concentration was measured using the Qubit (Thermo Fisher Scientific, Waltham, MA, US), and RNA integrity was analyzed using RNA Pico chips on 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, US). The RNA was converted to cDNA and pre-amplified using the SMARTer Ultra Low RNA kit (Clontech Laboratories, Montain View, CA, US) using 0.5-2ng total RNA input. A sequencing library was generated from 1ng (Qubit measured) amplified cDNA using the Illumina Nextera XT kit (Illumina, San Diego, CA, US).
attributes.tag: GEO Accession
attributes.value: GSM3212633