title: GSM2724550: SVA-ZN5; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from NPCs was extracted using the RNeasy Micro Kit (Qiagen), treated with TURBO DNase (Ambion) for 1 h at 37 °C and then re-purified with the Qiagen RNeasy Micro Kit. RNA samples were quantified using the Qubit RNA HS Assay Kit (Thermo Fischer Scientific) their purity was evaluated on a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and the integrity was verified using the Agilent RNA 6000 Pico Kit (Agilent Technologies) in a 2100 Bioanalyzer Instrument (Agilent Technologies). RNA samples from NPCs were used to prepare tagged sequencing libraries with the KAPA Stranded mRNA-Seq Kit (Illumina), according to the manufacturer’s instructions; then sequenced on the Illumina HiSeq4000 using 150 bp paired-end reads sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2724550