title: RNA of Phellinus noxius: fruiting body biological replicate 1 (fruitRep1)
design: For transcriptome sequencing, high quality total RNAs samples for selected based for high purity (NanoDrop and Qubit RNA assay) and integrity (Agilent BioAnalyzer 2100 RNA 6000 Nano assay).  Input of 2 ug total RNA was subjected to olido-dT purification and library construction using the Illumina TruSeq RNA Library Prep Kit V2 with single index barcoded adaptors wity reduced PCR cycles to minimize bias. Sequencing was carried out on HiSeq PE2*`101.  All NGS preparation and sequencing were conducted at the High Throughput Genomics Core at BRCAS, Academia Sinica, Taiwan.