title: 16s rRNA of intertidal carbonate endolithic microbiome: survey site 10
design: 16S rRNA gene V3/V4 variable region PCR primers 341F/806R with barcode on the forward primer were used in a 28 cycle PCR. After amplification, PCR products from  multiple samples are pooled together in equal proportions based on their molecular weight and DNA concentrations. Pooled samples are purified and then used to prepare illumina DNA library