title: D5 FLX cDNA
design: RNA was provided to Washington University in St. Louis to develop 454 EST sequences on the FLX platform.  cDNA was poly-T primed and amplification adaptors included modified SMRT sequences that allowed for trimming of the poly-A (or -T) sequences prior to sequencing based on an MmeI cut site.  Once the cDNA was SMRT amplified, the library was normalized using Evrogen's Trimmer kit (DSN).  A sample (0.5 FLX plate) was sequenced using FLX technology (prior to Titanium) and the raw reads contain some adaptor sequences. These reads are in file FEMR26K01.sff