title: GSM2460317: p6_t2; Homo sapiens; MBD-Seq
library_construction_protocol: Peripheral blood was collected in EDTA tubes at both the baseline and follow up from non-fasting subjects, and frozen immediately and stored at -80°C until use. Peripheral blood mononuclear cells (PBMCs) were isolated from the collected blood. Genomic DNA was extracted from PBMCs using QIAamp DNA Blood Maxi Kit (Qiagen; Hilden, Germany) according to the manufacturer’s protocol. In order to obtain the methylated DNA, 2.5μg of isolated genomic DNA was fragmented down to 250 bp using a M220 Focused-ultrasonicator (Covaris; Woburn, MA), then 1μg fragmented DNA was processed with MethylMiner Methylated DNA Enrichment kit (Invitrogen; Carlsbad, CA). Enriched 10ng of methylated-DNA was used for a DNA library preparation with TruSeq Nano DNA kit (Illumina; San Diego, CA).
attributes.tag: GEO Accession
attributes.value: GSM2460317