title: GSM2980787: Monocyte_donor_08; Homo sapiens; RNA-Seq
library_construction_protocol: Mononuclear cells (PBMCs) were isolated from peripheral blood of healthy adult donors (Department of Transfusional Medicine, University Hospital of Padova) with LymphoprepTM density gradient centrifugation (STEMCELL Technologies, Vancouver, BC, Canada) followed by haemolysis (0.5M EDTA pH 8.0). CD45+ population was separated by sorting with FACS AriaTM III (Becton Dickinson, NJ07417, USA) into B-cells, T-cells and monocytes, after labeling with anti CD19, CD3, CD14 antibodies (Beckman Coulter, CA 92821, USA). Total RNA was extracted with TRIzol reagent (Thermo Fisher Scientific, Waltham, Massachusetts) and precipitated with isopropanol. Samples with RNA integrity number (RIN) > 7, assessed with Agilent 2100 Bioanalyzer (Santa Clara, CA, USA), were used for sequencing. RNA library was prepared with TruSeq Stranded Total RNA Ribo-Zero Gold kit. Paired-end reads 100–125 bp
attributes.tag: GEO Accession
attributes.value: GSM2980787