title: GSM4260586: Day_3_Control_3_R2_B9; Homo sapiens; RNA-Seq
library_construction_protocol: Day 0 myoblasts were lifted from plates using trypsin, washed, and captured on medium IFCs (10-17 um). Day 3 myotube single nuclei were isolated from mononucleated cells (MNCs) by washing a 6 cm dish once with trypsin, then adding trypsin for about 5 min until myotubes lifted off the plate and MNCs were still attached. Cells were initially pelleted at 2000 rpm for 2 min and resuspended in lysis buffer with 0.02% IGEPAL CA-630. Lysis was done for 3 minutes, filtered and spun at 4000 rpm for 1 minute. Nuclei were captured on medium IFCs (10-17 um) at a density between 340 and 640 nuclei/ul in a volume of 10 ul. cDNA was normalized to approximately 0.1 ng/ul for tagmentation and library prep (Illumina, FC-131-1096). Libraries were sequenced on the Illumina NextSeq500 platform using paired-end 75 bp mode with 1-3 million reads per sample for full length RNA-seq single-cell and single-nucleus libraries.
attributes.tag: GEO Accession
attributes.value: GSM4260586