title: GSM3214207: C3_inflamed; Homo sapiens; RNA-Seq
library_construction_protocol: After study ethical approval fresh intestinal biopsies were collected during colonoscopy  in those undergoing routine / screening colonoscopy (Healthy) of in those with known ulcerative colitis (UC). Samples were transported on ice and epithelial crypts isolated using a chelation medium (HPGA with 1mM EDTA) and then isolated crypts digested to a single cell solution (TryPLE Express with DNAse) before being processed in 10X chromium as per manufacturers protocol. Library construction by 3' prime V2 chemistry Chromium single cell gene expression kit. 
attributes.tag: GEO Accession
attributes.value: GSM3214207