title: lung microbiome from sputum for E15_57_2.1_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ACGCTCGACA for subject 57 at a Exacerbation visit on 03_03_08