title: GSM3137806: Wildtype_4; Mus musculus; RNA-Seq
library_construction_protocol: Islets were isolated as described above and incubated over night at 37 degree centigrade. The next day, 500 islets per genotype were picked into Trizol (Invitrogen) as one sample and kept at -80 degree centigrade until RNA extraction and sequencing. 5 different WT samples and 4 different R138X samples were analyzed. Total RNA was purified from all samples using MagMAX™-96 for Microarrays Total RNA Isolation Kit (Ambion by Life Technologies) according to manufacturer's specifications. Genomic DNA was removed using MagMAX™Turbo™DNase Buffer and TURBO DNase from the MagMAX kit listed above (Ambion by Life Technologies). mRNA was purified from total RNA using Dynabeads mRNA Purification Kit (Invitrogen). Strand-specific RNA-seq libraries were prepared using KAPA mRNA-Seq Library Preparation Kit (Kapa Biosystems). Twelve-cycle PCR was performed to amplify libraries. Sequencing was performed on Illumina HiSeq®2500 (Illumina) by multiplexed single-read run with 33 cycles.
attributes.tag: GEO Accession
attributes.value: GSM3137806