title: GSM4259497: Sample_12hr-rep3; Spodoptera frugiperda; RNA-Seq
library_construction_protocol: Total RNA was isolated from the entire body of each healthy or SfAV-1a infected Spodoptera frugiperda larva and homogenized separately by mechanical trituration in TRIzol (Invitrogen), followed by DNase-treatment using the RNA Clean and Concentrator TM-5 kit (ZYMO RESEARCH) and eluted in ultra pure distilled water (Invitrogen). The NEBNextPoly(A) mRNA magnetic isolation module kit (New England Biolabs) was used to enrich the mRNA first from the DNase-treated total RNA samples. The libraries were then prepared following the manufacturer's instructions of the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs)
attributes.tag: GEO Accession
attributes.value: GSM4259497