title: GSM3670770: RNA-seq-Diamide_Hsf_0min; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells (1 mL) were pelleted and flash frozen in liquid nitrogen. Pellets were resuspended in 300 uL TRIzol and lysed with ~1 mL ceramic beads on a Fastprep-24 (MP Biomedicals). Cell debris were pelleted and RNA was extracted from the supernatant using the Direct-Zol RNA Microprep Kit (Zymo Research). RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB Cat # E7420)
attributes.tag: GEO Accession
attributes.value: GSM3670770