title: lung microbiome from sputum for 58A_106_4_Stable
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode CGTGTCTCTA for subject 106 at a Stable visit on 10_10_08