title: GSM4310271: mSTARR_pcDNA_3; Mus musculus; OTHER
library_construction_protocol: 48 hours after transfection, total RNA was isolated using the Qiagen RNeasy maxi prep kit (Qiagen; 75162). polyA+ RNA was isolated using Dynabeads Oligo-dT25 (ThermoFisher Scientific; 61002) and treated with Ambion turboDNase (ThermoFisher Scientifc; AM2238) for 30min at a maximum concentration of 150ng/μl. RNA was purified using the Qiagen RNeasy MinElute kit (Qiagen; 74204). First-strand cDNA synthesis and library preparation was performed as described (Arnold et al., Science 2013). Library quality and concentration were assessed by Bioanalyzer (Agilent) and KAPA Library Quantification Kit (KAPA Biosystems; KK4824). Libraries were pooled equimolarly before sequencing on a Miseq (PE150).
attributes.tag: GEO Accession
attributes.value: GSM4310271