title: GSM4158854: WT4DZ; Mus musculus; RNA-Seq
library_construction_protocol: Popliteal lymph nodes from immunized chimeric mice were harvested and processed for flow cytometry analysis. Cell suspensions were stained and sorted into 50µl of lysis/binding buffer (Life Technologies). mRNA was captured with 12 ml of oligo(dT) Dynabeads (Life Technologies), washed, and eluted at 85°C with 10 µl of 10 mM Tris-Cl (pH 7.5). A variation of MARS-seq was used as described (Jaitin et al., 2014), developed for single-cell RNA-seq to produce expression libraries.
attributes.tag: GEO Accession
attributes.value: GSM4158854