title: bacterial diversity in 40 venous leg ulcers
design: Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) was performed as described previously [21] at the Research and Testing Laboratory (Lubbock, TX.).  The new bacterial tag-encoded Titanium amplicon pyrosequencing (bTETAP) approach is based upon similar principles to bTEFAP but utilizes Titanium reagents and titanium procedures.  The bTETAP procedures were performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com).