title: RNA-seq of juvenile Spirinchus thaleichthys: whole fish (pooled sampled to assist in transcriptome assembly): Sample Longfin smelt_Wild
design: "Three wild fish were sequenced to aid in transcriptome assembly. Whole fish were homogenized using a TissueLyser (Qiagen) in Buffer RLT Plus (RNeasy Plus Mini Kit; Qiagen) and 350 ?l of the homogenate was used for the RNA extraction on a Qiacube (Qiagen) following manufacturer’s protocols. RNA quality was assessed using a Bioanalyzer (Agilent) and concentration was measured using a Qubit Fluorometer (Life Technologies). 1.5 ?g of total RNA from each fish was pooled and used to generate one Poly-A isolated, strand-specific cDNA library using a Directional PrepX RNA Library Prep kit (Wafergen Biosystems).  This library for longfin smelt was pooled with the library for the hatchery fish and sequenced on an Illumina HiSeq 2500 over two lanes. Sequencing was conducted to produce 100 base pair paired-end reads. 