title: GSM2805413: input DNA, HPAEC; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin isolation, sonication, immunoprecipitation using anti-SMAD1/5 antibody (913C1b; Bio Matrix Research, Chiba, Japan) and anti-FLAG M2 antibody (F3165; Sigma-Aldrich, Merck) were performed essentially as described previously (Morikawa et al., 2016). For cells stably expressing FLAG-ATOH8, we conducted nuclei isolation by NEXSON (Nuclei EXtraction by SONication) (Arrigoni et al., 2016) with slight modifications in order to reduce possible non-specific binding of exogenous protein. The libraries were constructed as described (Murai et al, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).
attributes.tag: GEO Accession
attributes.value: GSM2805413