title: GSM3731611: C1; Homo sapiens; miRNA-Seq
library_construction_protocol: After collected, samples were put in liquid nitrogen quickly, and preserved in low temperature environment. TRIzol (Invitrogen, Waltham, Massachusetts, USA) was used to isolate total RNA from tissue samples following a manufacturer's protocol. Total RNA quantity was determined with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA) and quality was assessed by running an 1% agarose gel electrophoresis, stained with 4S Red Plus Nucleic Acid Stain (Sango, Shanghai, China). miRNA-seq libraries were prepared using the TruSeq® Small RNA Library Preparation kit from Illumina® and sequenced using a 50 bp single-end reads Illumina NextSeq 500 Sequencer (Illumina Inc., San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM3731611