title: GSM2205514: D6 5µM GSK126; Mus musculus; RNA-Seq
library_construction_protocol: RNA (RNA-Seq) was isolated using the miRNeasy mini kit (Catalog # 217004, Qiagen). DNA (ChIP-Seq) was isolated from ChIP assays with the use of Phenol: Chloroform: Isoamyl Alcohol (25:24:1 v/v) solution (Invitrogen, Catalog 15593-031). The DNA was then precipitated overnight using 0.3M sodium acetate  in the presence of 20µg of glycogen in 70% ethanol. The DNA was then spun down, washed with 70% ethanol, and dissolved in 50µl of water. RNA-Seq libraries were prepared using the Illumina TruSeq v2 mRNA protocol. ChIP-Seq libraries were prepared using the NEBNext ChIP-Seq Library prep kit for Illumina sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2205514