title: GSM3904207: mock4; Mus musculus; RNA-Seq
library_construction_protocol: Embryos were washed with several drops of media and PBS, and processed as in Poulain, S. et al., . Methods Mol Biol, 2017 PMID: 28349422. In short, embryos were lysed in 5 ul lysis buffer containing an RT primer (TCGTCGGCAGCGTCAGATGTGNNNNNN). Samples were then reverse transcribed in a 20 ul RT reaction containing a template-switching oligo (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNTATA(rG)(rG)(rG)). Pre-amplification PCR was performed using a PCR primer (TCGTCGGCAGCGTCAGATGTG). The concentration of the resulting cDNA libraries was measured with Qubit® 2.0 Fluorometer (ThermoFisher Scientific) and library preparation was performed with the Nextera XT Library Preparation Kit (Illumina, Cat. FC-131-1096), followed by library fragment size validation with BioAnalyzer (Agilent Technologies).
attributes.tag: GEO Accession
attributes.value: GSM3904207