title: GSM2879360: Org1; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were prepared according to the Sort-seq method as described previousy.Briefly, Epcam+ primary kidney cells, FACSsorted (Facsjazz, BD) with Alexa-fluor 488 anti-human CD326 (Epcam, clone9C4; Biolegend), and the single cell suspension from passage 4 tubuloids (derived from the same tissue as the Epcam+ primary kidney cells) were single cell sorted into 384 well plates (Biorad) containing 5 µl vapor lock (Qiagen) 100-200 nl of RT primers, dNTPs and synthetic mRNA spike-ins. Live single cells were selected on the basis of DAPI and forward/side scatter properties. After the sort, plates were spun down and frozen to -80 degrees until further processing. RNA samples were processed into cDNA libraries as described previously and on the basis of the CEL-seq2 technique. Illumina sequencing libraries were prepared with the TruSeq small RNA primers (Illumina) and sequenced paired-end at 75 bp read length the Illumina NextSeq. Primary cells and tubuloid cells were processed and sequenced in parallel.
attributes.tag: GEO Accession
attributes.value: GSM2879360