title: Arabis alpina root microbiota L1264
design: Plant roots were mechanically separated from the adhering soil particles and a defined root segment was sampled. Root and rhizosphere compartments were separated by sequential washing steps. Soil samples were collected from unplanted pots. From the soil, rhizosphere and root compartments DNA was isolated using the FastDNA ® SPIN for soil kit. The 16S rRNA gene was amplified from individual samples using the primers 799F and 1193R. Different samples were amplified using primers containing different barcodes and a maximum of 96 samples was pooled per sequencing library.