title: Water sample from Sept. 2017 E. affinis laboratory feeding experiment
design: Amplified ITS region 1 by custom primers with dual 8 bp indices, Illumina TruSeq sequencing primer binding sites, and the P5 and P7 Illumina adapters.  PCR amplified fragments were indexed, pooled, and cleaned using Agencourt AMPure beads. The final, cleaned pool was quantified in duplicate using the Illumina Library Quantification qPCR Kit and sequenced at 11pM with Illumina MiSeq 300PE v3 chemistry.