title: GSM3592128: PRMT1_NesCre_Whole brain_(ffCre)_#2; Mus musculus; RNA-Seq
library_construction_protocol: Prmt1flox/flox; Nes-Cre mice were generated as previously described (Hashimoto M. et al., J. Biol. Chem. 2016, doi: 10.1074/jbc.M115.684514). Whole brains, cortices or cerebella were frozen in liquid nitrogen and were crushed into powder with a Multi-beads Shocker (Yasui Kikai Co.). Total RNA was extracted using ISOGEN II reagent (Nippon Gene). 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs), and then converted to Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Reads were mapped on GRCm38 (mm10) mouse reference genome and quantified using CLC Genomics Workbench version 12.0 (QIAGEN). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3592128