title: lung microbiome from sputum for 72A_161_5_Stable
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode CGTGTCTCTA for subject 161 at a Stable visit on 07_01_10