title: GSM3368727: pmk-1(km25) OP50 2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: After 3 washes in M9 buffer, TRIzol Reagent (Invitrogen) was added to worm pellets and flash frozen in liquid nitrogen. After an additional round of freeze-thaw, RNA was isolated using the Direct-zol RNA MiniPrep kit (Zymo Research) Libraries prepped using Kapa mRNA Hyperprep kit. Paired end reads sequenced on Illumina NextSeq500 sequencer, with v2 high-output chemistry.
attributes.tag: GEO Accession
attributes.value: GSM3368727