title: 16S bacterial metadata
design: DNA sequences were amplified using primers 515/806. Sequences were later joined, barcodes and minuscule reads were removed, while the remaining sequences were denoised.  Chimeras were removed as OTUs generated with 97% homology and were classified using the Ribosomal Database Project(RDPII) and National Center for Biotechnology Information (NCBI) derived database.