title: Amplicon-Seq of STP02
design: Microbial community DNA was extracted from the anaerobic digester                 sample using a modified phenol-chloroform extraction method and then 16S amplicons                 were generated with the primerset 515YF (5´-GTGCCAGCMGCCGCGGTAA-3´) and 806R                 (5´-GGACTACHVGGGTWTCTAAT-3´) as suggested by Klindworth et al. (2013). Sequencing                 was performed by Mcirosynth AG (Balgach, Switzerland) with the                 MiSeq-technology. Paired-end reads were merged to a single read.