title: GSM4158364: MCF10A_ATAC_WT_1; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq was performed as previously described (Buenrostro 2013). Briefly, cells were lysed and nuclei counted. Transposition reaction was performed on 105 nuclei using 5l of Nextera TDEI enzyme (Illumina) for 30 minutes at 37°C Library was amplified using NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs). Library concentration was measured by DNA High Sensitivity Kit (Invitrogen) on a Qubit fluorometer (Invitrogen). Library quality and fragment sizes were assessed on a Bioanalyzer (Agilent)
attributes.tag: GEO Accession
attributes.value: GSM4158364