title: GSM2980690: CTCF-F1-F9.bound; synthetic construct; OTHER
library_construction_protocol: All single stranded DNA library template were mixed in equal molar ratio, annealled by iPE2.0-reversed primer and further extended by Taq Polymerase at 95C 15s, 52C 10mins. After EMSA, DNA fragments were cut and extracted by gel extraction buffer at 50C for 30mins. For each portion of DNA fragment, PE1 and PE2 PCR primers were used to PCR amplify extracted DNA for 13 cycles (95C 15s, 52C 15s, 72C 45s)
attributes.tag: GEO Accession
attributes.value: GSM2980690