title: GSM4658980: Normal 1; Homo sapiens; RNA-Seq
library_construction_protocol: Adult human testis biopsies were obtained from three normal and three cryptozoospermic patients undergoing therapeutic testicular sperm extraction. For each patient, a single cell suspension was obtained using a two-step enzymatic digestion. 12.000 cells per patient (normal=3; crypto=3) were resuspended in MEMα at a concentration of 500 cells/μl and loaded onto the Chromium Single Cell A Chip. The library preparation was performed following the kit instructions (Chromium Single Cell Kit [v2 chemistry]). Briefly ~6.000 cells per patient were captured by the 10x Genomics Chromium controller, after cDNA synthesis, 12-14 cycles were used for library amplification. The resulting libraries were quantified using the Lab901 TapeStation system (Agilent) before proceeding with shallow sequencing on a NextSeq-550 sequencer (Illumina) as a quality control measure. The final, deep sequencing was performed on a NovaSeq 6.000 sequencer (Illumina), using 2 × 150 bp paired-end sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4658980