title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of human ovarian cancer cell line OVSAHO parental and CB-5083-resistant cells
design: RNA-seq of human ovarian cancer cell line OVSAHO parental and CB-5083-resistant cells
library_construction_protocol: Cells were treated with CB-5083 (5 microM) or DMSO for 6 hours. Each group has triplicate samples. After treatment, cells were washed with ice-cold PBS, and lyzed in Trizol. RPMI 1640 with 10% fetal bovine serum 5 microM CB-5083 for 6 hours Total RNA was extracted using Trizol according to manufacturer's protocol. Library was constructed using Illumina TruSeq RNA Sample Preparation kit v2.
attributes.tag: Experimental Factor: phenotype
attributes.value: sensitive to CB-5083
attributes.tag: Experimental Factor: compound
attributes.value: CB-5083
attributes.tag: Experimental Factor: dose
attributes.value: 5
attributes.tag: Experimental Factor: time
attributes.value: 6