title: Aplysia Fragile X cargo analysis
design: Fragile X cargo was isolated by a column and bound RNA isolated. Library construction starts with total RNA reverse transcribed to cDNA with an oligo dT primer, with second strand then generated. The cDNA is digested with a restriction enzyme then adaptors ligated onto the double-stranded (ds) DNA.  The adaptor ligated cDNA fragments are the amplified by PCR. This product is purified and processed for emPCR and 454 sequencing according to manufactures procedures.