title: lung microbiome from sputum for 20A_79_4_Stable
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode TGTACTACTC for subject 79 at a Stable visit on 08_06_08