title: GSM2266567: 2_1NC; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using Tri Reagent and subsequently treated with DNase 1. TruSeq RNA Sample Prep Kit per the manufacturer’s protocol. Briefly, mRNA was purified from total RNA using oligo dT magnetic beads, fragmented and then reverse transcribed via random hexamers and SuperScript II Reverse Transcriptase.  Second strand cDNA was synthesized using a proprietary master mix.  The double stranded cDNA was then blunt ended, the 3’ ends were adenylated and indexed Illumina adaptors were ligated.  DNA fragments were enriched by PCR using the following conditions: initial denaturation of 98° C for 30 seconds, 15 cycles of 98° C for 10 sec, 60° C for 30 sec and 72° C for 30 sec, followed by a final extension of 72° C for 5 min.  RNA-seq libraries were visualized by Bioanalyzer and quantified by qPCR.  Libraries were pooled by quantity and sequenced via 50 bp single reads on a HiSeq2000.
attributes.tag: GEO Accession
attributes.value: GSM2266567