title: GSM4478694: K562 EU-seq DMSO 2 hours DG4D; Homo sapiens; OTHER
library_construction_protocol: EU-RNA was enriched using Click-iT Nascent RNA Capture Kit (Invitrogen), with 5 µg of EU-RNA for biotinylation by click reaction and 1 µg of biotinylated RNA for binding to Streptavidin T1 magnetic beads. cDNA was generated using iScript Select cDNA Synthesis Kit (Bio-Rad) and cleaned up before 2nd-strand synthesis. cDNA was ligated to Illumina barcoded adapters for sequencing. Library quality was checked on an Agilent BioAnalyzer and sequencing was performed on Illumina HiSeq or NovaSeq instruments.
attributes.tag: GEO Accession
attributes.value: GSM4478694