title: GSM3464405: D1_control ipsilateral; Mus musculus; RNA-Seq
library_construction_protocol: After sacrificing brains were removed, stroke lesion area and the corresponding contralateral area were isolated. CD31+ cells from these tissues were first enriched with the MACS technique. Afterwards followed an isolation of CD45-/DAPI-/CD31+/CD146+ single cells by flow cytometry. RNA isolation was performed with the NucleoSpin® XS Kit (MACHEREY-NAGEL; Prod#: 740902.50). The library construction was performed with the Encore Rapid DR Multiplex system from NuGen Technologies, Inc. according to the manual.
attributes.tag: GEO Accession
attributes.value: GSM3464405