title: GSM2266290: Calu-3 Input; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were lysed in lysis buffer (5mM PIPEs pH 8.0, 85mM KCL, 0.5% NP-40, 1x Protese Inhibitor Cocktail (Roche)).  Nuclear preparation was isolated by centrifugation, lysed in RIPA buffer (1x PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Protease Inhibitor Cocktail), and sonicated. Protein-DNA complexes were isolated with antibody. DNA ends were blunted using T4 DNA polymerase, Klenow DNA polymerase, and T4 polynucleotide kinase. DNA was then incubated with Klenow exo- to add 5’ adenine overhangs. Multiplex adaptors were ligated to the ends and converted to dsDNA using 5 cycles of PCR.  DNA was size-selected to contain fragments between 200 and 300bp and PCR amplified for 5 cycles.  DNA was purified with 1.2x AMPusreXP beads. Sequencing was performed on an Illumina Hi-Seq.
attributes.tag: GEO Accession
attributes.value: GSM2266290