title: GSM2980773: parS1-4_ChIP_1; Pseudomonas aeruginosa; ChIP-Seq
library_construction_protocol: Cells were fixed with formaldehyde washed and resuspended in lysis buffer, followed by sonication to obtain DNA fragments with an average fragment lengthsize 250-350. Cleared lysates were incubated with affinity purified polyclonal rabbit anti-ParB antibodies, followed by recovery of antibody-ParB-DNA conjugates using protein A coupled with magnetic beads. Complexes were decrosslinked and  the ChIP DNA was purified using a MinElute PCR purification kit. Libraries were prepared according to NEB instructions accompanying the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, E7370S). The libraries were sequenced using HiSeq4000 (Illumina) in the PE100 mode.
attributes.tag: GEO Accession
attributes.value: GSM2980773