title: GSM4408891: MSI-H, likely Lynch due to MSH2 germline, sample 1 normal tissue.; Homo sapiens; RNA-Seq
library_construction_protocol: Patient FFPE blocks were obtained from our tissue registry and after a pathologist review, samples with sufficient amounts of tissue were selected for RNA extraction from tumor and paired normal tissue. Total RNA was quantitated using the Qubit RNA BR assay (Invitrogen) and quality was assessed using the DV200 value (the percentage of RNA fragments greater than 200 bases in length) as determined by the Agilent 2100 Bioanalyzer RNA Nano kit.  For samples with a DV200>30% the RNA input for RNA-seq analysis was 200 ng.  For samples with a DV200 score between 20-30% the amount of RNA used was increased to 400 ng to compensate for RNA degradation.  Samples with a DV200 score below 20% were not used in the study since previous studies in our lab have shown that these rarely provide acceptable results.
attributes.tag: GEO Accession
attributes.value: GSM4408891