title: Solexa sequencing of AG library
design: In order to obtain cDNA fragments which cover the complete transcripts, prior to cDNA synthesis the RNAs were fragmented by incubating at 95 degree Celsius for 5 minutes. The RNA fragments were then poly(A)- tailed using poly(A) polymerase followed by ligation of a RNA adapter to the 5'-phosphate of the RNA fragments. First-strand cDNA synthesis was then performed using an oligo(dT)-adapter primer and MMLV reverse transcriptase. The resulting cDNAs were PCR-amplified to about 20 ng/*l using a high fidelity DNA polymerase. The primers used for PCR amplification were designed for amplicon sequencing according to the instructions of Illumina/Solexa. The following adapter sequences flank the cDNA inserts: 5'- end (53 bases) with Tag sequence 5'- AATGATACGGCGACCACCGACAGGTT-CAGAGTTCTACAGTCCGACGATCNNNN-3' 3'-end (45 bases): 5'- CAAGCAGAAGACGGCATACGATTTTTTTTTTTTTTTTTTTTTTTT-3'