title: GSM4146626: GFP-negative rep1; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was extracted from the sorted cells using the RNAqueous® Total RNA Isolation Kit (Ambion, Foster City, CA) and treated with Turbo DNase I (Ambion, Austin, TX) to remove residual genomic DNA. The treated RNA was then purified and concentrated using the RNA Clean & ConcentratorTM-5 Kit (Zymo Research, Irvine, CA). 1 ug of RNA was subjected to first-strand cDNA synthesis and cDNA amplification using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio, Kusatsu, Shiga, Japan). Purified cDNA was quantified using Qubit, and for each library 150 pg cDNA was used as input with the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA), following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4146626