title: GSM4260786: 7901NG (7901 TfR1 negative cells); Homo sapiens; RNA-Seq
library_construction_protocol: Cells were removed and sorted by FACS, then RNA was harvested using Trizol reagent. VAHTS total RNA-seq (H/M/R) library PrepKit for IIIumina (Vazyme, NR603-02) was used with 1 ug of total RNA for the construction of sequencing libraries. Follow the experimental instructions to perform the steps of rRNA removal, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3 'end plus A, ligation, and enrichment on the purified Total RNA to complete the sequencing sample library Construct (related productions were VAHTS total RNA-seq (H/M/R) library PrepKit for IIIumina, Vazyme, NR603-02; Agencourt AMPure XP Beads, Beckman, A63881; SuperScript II Reverse Transcriptase, Invitrogen, 18064014; Qubit dsDNA HS Assay Kit, Invitrogen, Q32854; Agilent High Sensitivity DNA Kit, Agilent, 5067-4626).
attributes.tag: GEO Accession
attributes.value: GSM4260786