title: Reseq of Phaseolus vulgaris:Young leaves
design: at least 6 g of genomic DNA was used to construct random, small insert sequencing libraries according to the manufacturers instructions for TruSeq nano DNA kit (Illumina Inc. Beijing). Insert DNAs were prepared by mechanical shearing using a Biorupter UCD-200 (Diagenode).  Adaptors containing the index/reading site for paired-end sequencing were ligated as per manufacturers instruction for Illumina. The libraries were amplified on a S1000 PCR Thermal cycler S1000 and evaluated on a Gel Doc XR system both from Bio-Rad (Hercules CA, USA). With the adaptors the sequencing libraries had an average insert size of approximately 300 to 350 bp and A StepOne Plus from ABI (Thermo Fischer Scientific, Waltham MA, USA) was used to evaluate quality of the libraries.