title: GSM2983324: Granisetron·HCl [HI.3146.002.BioO_2.46]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from cortical cultures using the NucleoSpin RNA kit (Macherey-Nagel). Purity and concentration of total RNA was tested by Nanodrop analysis while the RNA integrity number (RIN) of each total RNA sample was determined using the Agilent RNA 6000 nano kit and the Agilent 2100 Bioanalyzer. Complementary DNA (cDNA) libraries were created using the KAPA Biosystems Stranded mRNA-Seq kit from high-quality RNA samples (RIN≥8). Chemical fragmentation was performed per kit specifications to generate an average fragment size 200-300bps (confirmed by Agilent DNA 1000 kit). NEXTflex™ RNA-Seq barcode adapters were ligated onto fragmented RNA samples and adapter ligated libraries were amplified by polymerase chain reaction (PCR) for 10 cycles.
attributes.tag: GEO Accession
attributes.value: GSM2983324