title: lung microbiome from sputum for 101A_173_3.2_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode TGATACGTCT for subject 173 at a Exacerbation visit on 12_29_09