title: Illumina HiSeq 2000 paired end sequencing; Integrated host and viral transcriptome analyses reveal pathology and inflammatory response mechanisms to ALV-J injection in SPF chicken
design: Integrated host and viral transcriptome analyses reveal pathology and inflammatory response mechanisms to ALV-J injection in SPF chicken
library_construction_protocol: Slaughter, blood and immune organ were harvested on 3, 5, 7, 14, 24, 30, 40 days post injected (dpi). The phenotype of infected tissues (including appearance, viability, and the amount of viral mRNA and protein) was used to guide the selection of samples for RNA-seq.  At first day of age, ALV-challenged chicks were injected 100ul SCDY1 Avian leucosis virus subgroup J (supplied by College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, China) based on the TCID 50 of this virus, while a control group was injected 100ul PBS per chicks. Total RNA was isolated from the RNA later preserved spleen samples with miRNeasy Mini kit (QIAGEN, Gemany) in accordance to the manufacturer’s protocol. RNA was quantified using the NanoPhotometer® spectrophotometer (Implen Inc., CA, USA). We then measured RNA concentration using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA), and assessed RNA integrity using the RNA Nano 6000 Assay Kit in Bioanalyzer 2100 (Agilent Technologies, CA, USA). All the samples had a high RNA Integrity Number (RIN) score (>9.4).  A total of 3ug of RNA per sample was submitted to the Novogene Bioinformatics Technology Co. Ltd (Beijing, China) for library generation and sequencing.
attributes.tag: Experimental Factor: 24
attributes.value: age
attributes.tag: Experimental Factor: none
attributes.value: infect