title: GSM4314258: Ctrl_WT; Mus musculus; RNA-Seq
library_construction_protocol: Synovial dissociation followed protocol from Donlin, L. T. et al. Methods for high-dimensonal analysis of cells dissociated from cyropreserved synovial tissue. Arthritis Res. Ther. 20, 139 (2018). For scRNAseq experiments, viable cells in single cell suspension were resuspended in 0.4% BSA in PBS at a concentration of 1,000 cells per ul. 7,000 cells were loaded onto a single lane (Chromium chip, 10X Genomics) followed by encapsulation in lipid droplet (Single Cell 3' kit, 10X Genomics). cDNA and library generation per manufacturer protocol.
attributes.tag: GEO Accession
attributes.value: GSM4314258