title: GSM3464518: U937_Input-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were digested by micrococcal nuclease and DNA complexes were isolated with antibody A ChIP-seq library was prepared from approximately 20 ng of ChIP-DNA and an input sample from a mixture of 3 ChIP operations. The concentration and integrity of the DNA sample was checked by gel electrophoresis. The selected libraries for a fragment size of 100 bp to 500 bp were amplified using PCR. ChIP-seq libraries were run on a HiSeq next-generation sequencer (Illumina, San Diego, CA, USA). After abandonment of polluted reads, low-quality reads, and reads with N > 5%, sequenced-50 base short single reads were mapped to the human genome, and the peaks were identified by Model-based Analysis of ChIP-Seq S14 software.
attributes.tag: GEO Accession
attributes.value: GSM3464518