title: GSM2882181: TetO-YAP YAP; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were cross-linked in 1% formaldehyde for 10 min at room temperature after which the reaction was stopped by addition of 0.125M glycine. Cells were lysed and harvested in ChIP buffer (100 mM Tris at pH 8.6, 0.3% SDS, 1.7% Triton X-100, and 5 mM EDTA) and the chromatin disrupted by sonication using a Diagenode Bioruptor sonicator UCD-200 to obtain fragments of average 200-500 bp in size. Suitable amounts of chromatin were incubated with specific antibodies overnight. Immunoprecipitated complexes were recovered on Protein-A/G agarose beads (Pierce) and, after extensive washes, DNA was recovered by reverse crosslinking and purification using QIAquick PCR purification kit (QIAGEN). Libraries for ChIP-sequencing were generated by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and barcoded added using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB) according to manufacturer's recommendation.
attributes.tag: GEO Accession
attributes.value: GSM2882181