title: GSM3592236: Low BiFC sort; Replicate 2; Human immunodeficiency virus 1; OTHER
library_construction_protocol: Total RNA was harvested from sorted cells using GeneJet RNA Purification Kit (Thermo Scientific) and cDNA was prepared using Accuscript Hi-Fi (Agilent Genomics) primed with EBV reverse primer (GTGGTTTGTCCAAACTCATC). In a first round of PCR, the 001428 coding sequence was amplified using oligonucleotides with complementary overhangs for annealing to Illumina sequencing primers. Primer pair for gene-specific amplification: Illumina_001428VC_1996_for (TCTTTCCCTACACGACGCTCTTCCGATCTTTGGTCATGGTTTGATATCTC) and Illumina_001428VC_2277_rev (GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTTCTTCTGCTTGTCTCCAG) In a second round of PCR, Illumina adaptors and experiment-specific barcodes were added. Primer pair for adding I5 and I7 Illumina adaptors: MiSeq_Start_Adaptamer (AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT) and MiSeq_Index_Adaptamer (CAAGCAGAAGACGGCATACGAGAT-6nt-barcode-GTGACTGGAGTTCAGACGTGTGCTCTTC) Amplicons were deep sequenced on a MiSeq using a 2x300nt PE v3 paired end protocol.
attributes.tag: GEO Accession
attributes.value: GSM3592236