title: Illumina HiSeq 2500 paired end sequencing
design: Exome capture
library_construction_protocol: Genomic DNA was extracted from a single EMS-mutagenized M2 plant following a large-scale extraction protocol that includes an initial step of nuclei purification, followed by proteinase K and phenol-chloroform purification. Normalized DNA was sheared on Covaris E220 instrument. Genomic DNA libraries were constructed using HTP KAPA kit and Bioo-Netflex 96 barcoding oligoes 1-48. All library construction was done using Sciclone G3 robotics. A custom wheat exome capture was designed during the course of this project in collaboration with Nimblegen (Roche). The initial alpha design was tested on a subset of samples and the probes were re-balanced accordingly. The final beta design was used for the remainder of the project. The data deposited to this SRA experiment contains sample captured with alpha capture design.