title: GSM2549500: FCS 5d 2; Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA was isolated using the RNeasy Micro Kit (Qiagen) with on-column DNase treatment. For each replicate, 10 ng of total RNA (RIN > 9.7) was used to prepare a poly-A enriched cDNA library using the SmartSeq2 protocol. Libraries were modified for sequencing using the Nextera XT DNA Sample Preparation Kit (Illumina) with 150 pg of cDNA as input material. Libraries were sequenced using an Illumina MiSeq to obtain 75 bp paired-end reads. Data were collected in two batches of library preparation and sequencing, with samples from each experimental condition included in each batch.
attributes.tag: GEO Accession
attributes.value: GSM2549500