title: GSM4042202: sc_Donor_7_GEX; Homo sapiens; RNA-Seq
library_construction_protocol: For single-cell RNA seq library construction, cells were diluted to ~    1X10^6/mL in 100 uL PBS containing 0.04% BSA and processed within hours after they were obtained. Briefly, freshly sorted CD8+ T cells were kept on ice before processing. Viable cells counted and about 8700 cells were used for Gel bead-in-emulsion (GEM) generation (targeted recovery ~5000 cells). Single-cell RNA seq libraries were constructed using Single Cell 3' library (v2 Chemistry) and Gel beads kits (10x genomics) according to the manufacturer's protocol. After template switching reverse transcription in beads, cDNA was amplified in bulk. Subsequent fragmentation, end repair, A-tailing, adaptor ligation, and sample index PCR generated P5- and P7- capped, cell- and sample-indexed single-cell 3' libraries. Libraries were quantified using both bioanalyzer (Agilent) and Kapa library quantification kit (Roche) before sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4042202