title: GSM2882713: WT_ATAC_rep1; Mus musculus; ATAC-seq
library_construction_protocol: Limb buds were dissected, dissociated using 100ug/mL Liberase (Roche 05401119001) in PBS at 37 degrees for 6 minutes, and cells were counted. 5,000 cells per sample were lysed and immediately added to a transpose reaction for 30 min at 37C (Illumina FC-121-1030). After the transposase reaction was complete, the entire reaction was put into a PCR library amplification reaction. Barcoded primers, specific to the inserted transposon sequence, amplified accessible chromatin fragments. Fragments were amplified for 18 PCR cycles using NEBNext High Fidelity 2x PCR Master Mix (M0541S). Excess primers were removed from PCR reactions using AmpureXP beads (A63880) at a ratio of 0.8x beads.
attributes.tag: GEO Accession
attributes.value: GSM2882713