title: GSM4658421: 1.5_shC_DamLmnB1; Mus musculus; OTHER
library_construction_protocol: MEFs were collected by trypsinization and DNA was isolated using QIAamp DNA Mini kit (Qiagen,  51304), followed by ethanol precipitation and resuspension to 1 µg/ul in 10 mM Tris, pH 8.0.  DamID was performed as described previously (L.Guelen et al, Nature 2008) post-DamID amplified material was randomized  by performing an end repair reaction, followed by ligation and sonication.  Randomized fragments were subjected to sequencing library preparation with NEBNext Ultra DNA library prep kit
attributes.tag: GEO Accession
attributes.value: GSM4658421