title: ALD_CC_K03_07_RNA_Bac: 16S rRNA analysis of bacterial communities in sediment, water and biofilms
design: Metagenomic RNA was extracted from sediment, biofilms and water filters. Contaminant DNA was digested, the RNA purified and reverse transcribed into cDNA. The V3-V4 region of the 16S rRNA was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.