title: GSM4259420: WT leg disc; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA isolated in Tri-Pure reagent (phenol-chloroform), mRNA selected via 3 passes over Invitrogen oligo-dT magnetic beads, mRNA was fragmented using heat and fragmentation buffer, RT was performed with Invitrogen SSIII first strand synthesis system and second-strand labeled with dUTP. RNA libraries were prepared for sequencing by the IMP sequencing core facility (Vienna, Austria) using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4259420