title: GSM2109614: H2085_DAC; Homo sapiens; miRNA-Seq
library_construction_protocol: total RNA was isolated with TRIzol following manual instructions Small molecular weight fraction (<200 bp) RNA was size fractioned using the Ambion (Life Technologies) flashPAGE fractionator to obtain small RNAs of 10-40nts. Small RNA sequencing libraries were created using SOLiD Small RNA Expression Kit (Ambion) starting with 200 ng of the size-fractionated material following the manufacturer’s instructions.  Libraries were sequenced on an Applied Biosystems (Life Technologies) SOLiD System to obtain 36bp reads.
attributes.tag: GEO Accession
attributes.value: GSM2109614