title: GSM3730719: GoldCLIP_dNxf2Halo_Lacz_KD_CLIP_rep1; Drosophila melanogaster; RIP-Seq
library_construction_protocol: Lysates were lysed in PBS supplemented with 0.2% Triton, dounced 30 times, clarified from debri, and HaloTag-protein-RNA complexes were isolated using HaloTag Beads. Briefly, RNA in the Halo-RBP-RNA complex was released and reverse transcribed. Then cDNAs were gel purified and amplified by PCR to add Illumina adaptors. The final libraries were purified and sequenced on Illumina X Ten platform.
attributes.tag: GEO Accession
attributes.value: GSM3730719