title: GSM3731856: RNA_THP1_0.5hrPMA_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: THP1 cells were harvested from each PMA stimulation time point by pelleting approximately 1E6 cells at 4°C, 500 R.C..F for 5 minutes. After removal of supernatant, cell pellet was homogenized with Trizol by repeatedly pipetting up/down with a 1mL micropipette tip. RNA was purified according to the Triozol manufacturere protocol. Libraries were prepared using manufacturer protocols in the SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian kit available from Takara Bio USA, Inc (catalog # 634873)
attributes.tag: GEO Accession
attributes.value: GSM3731856