title: lung microbiome from sputum for 78A_219_2_Stable
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode AGCACTGTAG for subject 219 at a Stable visit on 02_12_10