title: GSM3822282: GPR35-negative rep2; Mus musculus; RNA-Seq
library_construction_protocol: Intestines were removed, opened longitudinally and washed with PBS. Epithelial cells were removed by incubating in 5 mM EDTA 3x10 min at 37 C. The tissues were digested in RPMI 1640  containing 0.5 mg/ml Collagenase type VIII and 10 U/mL DNase 3x10 min at 37 C to obtain single cell suspension of lamina propria cells. GPR35-positive and -negative MHCII+CD64+ macrophages were sorted by FACS and lysed with trizol. RNA were extracted using Thermo Fisher PureLink RNA micro kit. RNA was reverse transcribed and cDNA amplified with SMART-Seq v4 (Takara) and libraries prepared with Nextera XT (Illumina) according to manufacturer's instructions.