title: GSM4658486: Post 9h_activin_Rep3; Xenopus laevis; RNA-Seq
library_construction_protocol: 10 animal caps per sample, in triplicate, were homogenized into ISOGEN (Nippon Gene) at 25℃.  Total RNA was extracted according to the manufacture's protocol. Extracted total RNA was purified by using Rneasy Mini kit (QIAGEN), and eluted RNA was mixed with 2.5 volume iced ethanol and NaCl (final concentration, 33mM) for ethanol precipitation.  Precipitated RNA was centrifuged at 4℃ for 30 minutes.  After removal of supernatants, RNA pellets were washed with 80% ethanol.  RNA pellets were dried up and resuspended with RNase free water. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4658486