title: GSM3135558: fumi_dwetA; Aspergillus fumigatus; RNA-Seq
library_construction_protocol: 2-day-old conidia of WT and DwetA strains were harvested from solid minimal media mRNA was extracted from total RNA using poly-A selection, followed by RNA fragmentation. The strand-specific library was constructed by first-strand cDNA synthesis using random primers, sample cleanup, and second-strand synthesis using DNA Polymerase I and RNase H. A single 'A' base was added to the cDNA fragments followed by ligation of the adapters. The final cDNA library was achieved by further purification and enrichment with PCR
attributes.tag: GEO Accession
attributes.value: GSM3135558