title: GSM4407860: Csm 5hpf Rep-3; Danio rerio; RNA-Seq
library_construction_protocol: 25 embryos each (for both StCsm and mock injected) were pooled at 128 cell stage, 5 hpf and 24 hpf from 3 biological replicates. Total RNA was extracted from the whole embryos with Tri Reagent Solution (Invitrogen) according to the manufacturer's protocol. RNA was further purified using Zymo RNA Clean and Concentrator-5 kit according to manufacturer's protocol. 1.5 ug of total RNA was subjected to Lexogen poly(A) RNA Selection kit according to company recommendations. Enriched RNA has been used for sequencing library construction with CORALL Total RNA-Seq Library Prep Kit according to the Lexogen manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM4407860