title: De Novo whole genome sequencing of Microbes
design: 35 ng of Qubit quantified genomic DNA was fragmented and adapter-tagged using Sure Select QXT Enzyme. Fragmented and adapter-tagged DNA was purified with High prep magnetic beads and then amplified and indexed in a 6 cycle PCR reaction. The final PCR product was purified with High Prep magnetic beads, followed by library quality control check.