title: GSM2983212: SS3_Mechlorethamine; Homo sapiens; RNA-Seq
library_construction_protocol: Cells treated for 24 hours under the 4 treatment conditions were washed with sterile PBS and flash frozen in liquid nitrogen. Total RNA was extracted from cell pellets by guanidinium thiocyanatephenol-chloroform extraction (TriZol, Sigma-Aldrich) and purified using RNeasy mini kit (QIAGEN, Germantown, MD, USA). Quantity and quality of isolated RNA were assessed using an Agilent Bioanalyzer 2100 and samples were stored at -80ºC. RNA-seq libraries were generated at the Sulzberger Genome Center at Columbia University Medical Center using the TruSeq Stranded RNA Library Prep kit with RiboZero gold (Illumina). Libraries were multiplexed and sequenced on an Illumina HiSeq2500 to obtain 100bp, paired-end reads to a target depth of 30 million reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM2983212