title: GSM3464878: plateA4.positionF2.single.cell; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were digest with Collagenase, Dispase and Elastase sollution, Single epithelial cell was sorted by BD AriaII. Cell was lysed by single cell RNA lysis buffer containing 0.45% (vol/vol) NP40, followed by reverse transcription using SuperScript II reverse transcriptase (Invitrogen) and whole transcription amplification using KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems). PCR product was purified using AMPure XP beads (Agencourt) and quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher). Libraries were constructed using illumina Nextera XT DNA sample preparation kit
attributes.tag: GEO Accession
attributes.value: GSM3464878