title: GSM1916108: Month28_1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized by cervical dislocation and the fur was trimmed before the skin was taken and placed into Ca2+ free PBS (d-PBS; Invitrogen). Subcutaneous tissue was removed, and the skin was floated on trypsin (T4424; Sigma-Aldrich) for 2 h at 32°C. After incubation, the epidermis was scraped into S-minimal essential medium (S-MEM; Invitrogen) supplemented with 0.01% soybean trypsin inhibitor (T9128; Sigma-Aldrich) and 0.5% BSA (bovine albumin fraction V solution (7.5%); Invitrogen) and gently mixed with a magnetic stirrer at room temperature for 20 min. The cell suspension was filtered through a 70-mm cell strainer (BD Falcon, Becton Dickinson, Franklin Lakes, NJ, USA), and viable cells were counted after staining with tryphan blue. Keratinocyte total RNA was thereafter  immediatel extracted with Trizol and RNA  integrity was assessed by the Agilent 2100 Bioanalyzer. The RNA quality criteria for proceeding with exon arrays were a 18S/28S ratio above 1.5 and an RNA integrity number (RIN-value) ≥ 8.0. For array hybridization and Whole Transcript Sense Target Labeling Synthesis, 200 ng of RNA was processed using the Gene Chip WT cDNA Synthesis Kit, (Affymetrix) Illumina standard RNA procedure
attributes.tag: GEO Accession
attributes.value: GSM1916108