title: GSM3464435: PTL3_OTH; Canis lupus familiaris; RNA-Seq
library_construction_protocol: RNA extraction was performed using the Purelink RNA mini Kit (Thermo Fisher Scientific, Waltham, MA) and quality was measured with an Agilent 2100 Bioanalyzer System. All samples had a RIN value greater than 8.80. RNA was shipped to Novogene Corporation Inc (Sacramento CA) where the libraries were constructed and sequenced. Briefly, mRNA was enriched using oligo(dT) beads and randomly fragmented. Random hexamer primer and reverse transcriptase were used for first strand cDNA synthesis followed by second strand synthesis via custom synthesis buffer (Illumina, San Diego, CA), dNTPs, RNAse H, and Escherichia coli polymerase I. Double-stranded cDNA fragments were purified and end-repaired by A-tailing and ligation of sequencing adapters. cDNA inserts were selected and enriched via PCR.
attributes.tag: GEO Accession
attributes.value: GSM3464435