title: WGS project for strain SA-152 Oxalobacteraceae bacterium
design: For the whole genome project of strain SA-152 the genomic DNA was extracted with the DNeasy PowerLyzer Microbial Kit (Qiagen) including an RNase A treatment at 37 C for 20 min. Illumina sequencing was performed by the Genomics Facility RTSF Michigan State University (USA) with the following main steps: library preparation using the SMARTer ThruPLEX DNA-Seq kit (Takara), quality control using a combination of Qubit dsDNA HS assay (Thermo Fisher Scientific) 4200 TapeStation High Sensitivity DNA 1000 assay (Agilent) and the Illumina Library Quantification qPCR kit (Kapa Biosystems), sequencing which was performed in a 2x250 bp paired-end format using a MiSeq Standard v2 flow cell and a MiSeq 500 cycle v2 reagent cartridge (Illumina).