title: GSM3822185: D7_Spleen_scRNA; Mus musculus; RNA-Seq
library_construction_protocol: For isolation of CD8+ T cells from spleen, spleens were collected and dissociated to yield a cell suspension prior to lysis of red blood cells. For isolation of CD8+ T cells from the small intestinal epithelium, Peyer's patches were removed and the tissue was cut longitudinally and washed of luminal contents. The tissue was then cut into 1cm pieces that were incubated while shaking in DTE buffer (1ug/ml dithioerythritolin 10% HBSS and 10% HEPES bicarbonate) at 37°C for 30 minutes. Cells within the supernatant were collected and passed through a 44/67% Percoll density gradient to enrich for lymphocytes. Single cell libraries were prepared according to the protocol for 10X Genomics for 3' Gene Expression. Approximately 10,000 sorted CD45.1+ Va2+ CD8+ CD44+ cells were loaded and partitioned into Gel Bead In-Emulsions (GEM). scRNA libraries were sequenced on a HiSeq4000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3822185