title: GSM2459942: DKO_3; Mus musculus; RNA-Seq
library_construction_protocol: Primary mouse enamel organ (EO) cells from the lower incisors were isolated and placed directly into RLT buffer (RNeasy Micro Kit (Qiagen). Cells were homogenized with a pestle and processed for RNA isolation with RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. RNA quality and quantity was analyzed on a Nanodrop2000 (Fisher Scientific) and RNAseq libraries were prepared using the TruSeq RNA sample prep v2 kit (Illumina), starting from 500 ng of DNase I (Qiagen) treated total RNA, following the manufacturer’s protocol. The amplified libraries (12 PCR cycles) were purified using AMPure beads (Beckman Coulter), quantified by Qubit 2.0 fluorometer (Life Technologies), and visualized in an Agilent Tapestation 2200. The libraries were pooled equimolarly, and loaded on the HiSeq 4000 Sequencing System (Illumina) and run as paired-end 150 nucleotide reads generating about 50 million reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM2459942