title: GSM4310378: DomB_r1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Two million of Kc167 cells were pelleted at 500 g for 5 min. Cells were resuspended in 1mL of PBS and 1 million of D. virilis 79f7Dv3 cells were added. Cells were pelleted at 500 g for 5 min and total RNA was extracted using RNeasy Mini Kit (QIAgen, Cat No. 74104), including DNase digestion step (QIAgen, Cat No. 79254). mRNA was purified using Poly(A) RNA selection kit (Lexogen, Cat. No M039100). Both total RNA and mRNA quality was verified on a 2100 Bioanalyzer (Agilent Technologies, Cat. No G2939BA). Libraries for sequencing were prepared using NEBnext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Cat. No E7760L).
attributes.tag: GEO Accession
attributes.value: GSM4310378