title: GSM3214018: KO_br3_Input; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Whole fly frozen with liquid nitrogen and ground in presence of lysis buffer containing heparin sulphate. Polysome profiling carried out as described with aliquot of initial loading material saved as "input" samples. After fractionation, fractions for monosome and polysome were identified and pooled appropriately. quant-seq 3' fwd kit was used to generate cDNA libraries from Input, Monosome, and Polysome fractions, with the addition of heparinase I in the early steps.
attributes.tag: GEO Accession
attributes.value: GSM3214018