title: GSM3592481: Kmt2d_mutant_DG_EdU_nuclei; Mus musculus; RNA-Seq
library_construction_protocol: Total nuclei (500 per genotype) were purified as described (Rizzardi et al., 2017) with addition of RNase inhibitor to all buffers, and EdU+ nuclei labeled by Click-it (Invitrogen) and isolated using Beckman Coulter MoFlo Cell Sorter Smart-seq2 protocol (Picelli et al., 2013) was modified using 22 cycles fo cDNA amplification and 20 enrichment cycles of tagmented fragments
attributes.tag: GEO Accession
attributes.value: GSM3592481