title: GSM4478382: ChIPseq_F1_H2AW7_rep1; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: ChIP-seq: Sixteen-days of seedlings grown on MS Agar plates were frozen in liquid nitrogen, ground, and fixed in 0.5% formaldehyde. Nuclei isolation, micrococcal nuclease (SIGMA N3755) treatment, nuclear membrane disruption were followed by centrifugation to extract the supernatant for immunoprecipitation. After affinity purification, DNA was reverse-crosslinked and purified. ChIP-seq: Libraries were prepared using SMARTer ThruPLEX DNA-seq Kit according to the manufacturer's instruction.
attributes.tag: GEO Accession
attributes.value: GSM4478382