title: GSM3819995: H4K91-2_ChIPSeq; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq data: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody; RNA-seq data: RNA was harvested using Trizol reagent ChIP-seq data: (1) DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptor.(2) PCR amplification and size selection (usually 100-300bp, including adaptor sequence). (3) Qualified library for sequencing. ; RNA-seq data: 1)mRNA enrichment: Oligo dT Selection or rRNA depletion;2)RNA fragment and reverse transcription: Fragment the RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random primer;3)End repair, add A tailing and adaptor ligation:The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments;4)PCR amplification:The ligation products were purified and many rounds of PCR amplification were performed to enrich the purified cDNA template using PCR primer;5)Denature and cyclization:Denature the PCR product by heat and the single strand DNA is cyclized by splint oligo and DNA ligase;6) Sequencing on BGISEQ-500 platform.
attributes.tag: GEO Accession
attributes.value: GSM3819995