title: RNA-seq of Arabidopsis seedlings: T-DNA insertion mutation treated with sorbitol for 36 hours
design: mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end.