title: NextSeq 500 sequencing; Herbal formula YYJD induces lung cancer cell apoptosis via activation of EGR1 (RNA-seq)
design: Herbal formula YYJD induces lung cancer cell apoptosis via activation of EGR1 (RNA-seq)
library_construction_protocol: A549 was maintained in RPMI 1640 medium (Corning) supplemented with 10% FBS (Gibco) and 100 units per ml penicillin–streptomycin solution at 37℃, 5% CO2 in a humidified incubator, then treated with anti-tumor drug YYJD.  Total RNA of untreated A549 and treated A549  were extracted using TRIzol regent (Ambio) according to the Ambion standard RNA isolation procedure, and resuspend in RNase free water.  Approximately 1ug Total RNA was purified using the NEBNext Poly (A) mRNA Magnetic Isolation Module (E7490, NEB). Libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420, NEB).
attributes.tag: Experimental Factor: compound
attributes.value: YYJD