title: Illumina HiSeq 4000 sequencing; RNA-seq of Mus musculus beta cells to determine the role of the miR-200–Zeb1 axis in beta cell tumor development
design: RNA-seq of Mus musculus beta cells to determine the role of the miR-200–Zeb1 axis in beta cell tumor development
library_construction_protocol: RT2 DKO cells were seeded in the evening, and cells were infected the next morning with adenovirus expressing either mir-141, mir-200c, mir-141~200c, or a control sequence at MOI 100. 48 hours later, cells were washed and harvested in TRI-Reagent.  RNA was extracted using TRI-Reagent, followed by RNeasy column purification and on-column DNase treatment. Quality was verified on Bioanalyzer TruSeq Stranded mRNA Library Prep Kit
attributes.tag: Experimental Factor: phenotype
attributes.value: mir-141/200c and mir-200a/b/429 homozygous knockout; mir 200c overexpression