title: GSM2266025: hPSC.SB.1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from FACS sorted AP+ve hPSC or NANOS3-tdTomato+ve hPGC using PicoPure® RNA Isolation Kit (Thermo Fisher Scientific) Total RNA (5 ng) was reverse transcribed and amplified into cDNA using Ovation RNA-Seq System V2 (Nugen). Amplified cDNA was sonicated into 250 bp by Covaris S220 Focused-ultrasonicators. Subsequently, RNASeq library was generated with 500 ng of fragmented cDNA using Ovation Rapid DR Multiplex System (Nugen). Library was quantified by qPCR using KAPA Library Quantification Kit (Kapa Biosystems).
attributes.tag: GEO Accession
attributes.value: GSM2266025