title: GSM4260238: EPC3-hTERT 0% alcohol; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lyzed in RLT buffer and RNA was isolated using Qiagen's Rneasy Minin kit. RNA was stored in -80 until further processing. Next-generation sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA library preparation kit, using high quality RNA (RIN >= 8.7) and 200ng of input. Libraries were assessed for quality using the PerkinElmer Labchip GX and qPCR using the Kapa Library Quantification Kit and the Life Technologies Viia7 Real-time PCR instrument. Libraries were diluted to 2nM and sequenced in a paired-end (2 x 100bp), dual-indexed format on the Illumina HiSeq2500 using the High Output v4 chemistry.
attributes.tag: GEO Accession
attributes.value: GSM4260238