title: GSM3822329: WTRC8BAT; Mus musculus; RNA-Seq
library_construction_protocol: Adipose tissue (iWAT and eWAT) of 16-week-old C57BL/6J mice (males) was digested with KRH buffer (2% FFA-free BSA) containing 3.3mg/ml collagenase I via incubation at 37 ℃ for 30-45 min. When the tissue was fully digested, 10 ml of KRH buffer was added to terminate collagenase activity. Cells were then filtered through a 250 μm cell strainer. After 10 min, the floating top layer containing mature adipocytes was collected. Mature adipocytes were washed twice with KRH buffer containing 5 mM EDTA and then used for RNA-seq analysis. Brown adipose tissues (BAT) were isolated and quickly freezed in liquied nitrogen. Total RNA extracted from mature adipocytes (iWAT and eWAT) and BAT tissue. RNAs with RIN >8 (assessed by the Agilent 2100 Bioanalyzer system) were used to prepare transcriptome libraries using the NEBNext Ultra RNA library prep kit (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM3822329