title: RNA_Seq of sour rice soup
design: Raw fastq files were quality-filtered by Trimmomatic and merged by FLASH with the following criteria: (i) The reads were truncated at any site receiving an average quality score <20 over a 50bp sliding window. (ii) Sequences whose overlap being longer than 10bp were merged according to their overlap with mismatch no more than 2bp. (iii) Sequences of each sample were separated according to barcodes (exactly matching) and Primers (allowing 2 nucleotide mismatching), and reads containing ambiguous bases were removed.