title: Illumin MiSeq squencing of planctomycetal acsA gene
design: Amplification of the acsA gene was done in a  nested PCR approach with external forward primer at position 1059 to 1078 (5«- GACDTGYTTGATGTACGAAG-3«), external reverse at position 1664 to 1682 (5«- TCSACYTCGATCGTGCTCA-3«), internal forward at the position 1120-1138 (5«- GTYGAGCGKCACAARGTCA -3«) and the internal reverse primer at the position 1626-1644 (5«- GATCACATCRTCGATGCGT -3«)