title: lung microbiome from sputum for 10B_44_2.12_Recovery
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode CTCGCGTGTC for subject 44 at a Recovery visit on 02_03_10