title: GSM4259472: D7_FACS; Mus musculus; RNA-Seq
library_construction_protocol: The tibialis anterior muscle was digested into a single-cell suspension in Collagenase D and Dispase II for about 1h, then filtered to remove debris. Red blood cells were lyzed. The single-cell suspension enriched for live cells by FACS using live/dead staining with PI/Calcein-AM. Single-cell RNA-sequencing libraries were prepared using a Chromium Single Cell 3' v2 Reagent Kit (10X Genomics) following the directions of the manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4259472