title: HSV-1 Strain F
design: From JVI 2010 paper by Szpara, Parsons, & Enquist:  Five micrograms of HSV-1 strain F and H129 nucleocapsid DNA were processed for sequencing by the Microarray Core Facility at Princeton University's Lewis-Sigler Institute for Integrative Genomics. Two independent sequence libraries were generated by following the manufacturer's protocol for sequencing of genomic DNA (Illumina Genomic DNA Sample Prep Kit; Protocol Part # 1003806 Rev. A), with the slight modification that the column for gel purification was not heated (Quail et al 2008, Nat Meth). Sequencing was carried out using two lanes of a standard flowcell, using Illumina's Standard Cluster Generation and 36-Cycle Sequencing Kits. The Illumina Genome Analyzer 2, with SCS 2.3 software, was run for either 36 (one H129 run) or 75 cycles (all other runs) of data acquisition. Image analysis and base calling was performed using the Illumina Pipeline v1.3 under default settings.