title: GSM4261190: RMB373_pTh17+IL12 OT2 WT NKG2D+; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified with NucleoSpin RNA Kit (Macherey-Nagel) from in vitro generated and FACS sorted Th1 and Th17 cells. RNA quality was determined on a Bioanalyzer using the RNA 6000 Nano Kit (Agilent). For poly-A dependent cDNA synthesis and a first amplification step the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) was used. After quality control (High sensitivity DNA Kit, Agilent) and concentration measurement (Qubit dsDNA HS Assay Kit, Invitrogen), 150 pg of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). In the following, 2x75 nucleotides paired-end sequencing was performed on a NextSeq 500 using a mid-output flow cell.
attributes.tag: GEO Accession
attributes.value: GSM4261190