title: GSM4407651: MOLM-13 A5-KD; Homo sapiens; ATAC-seq
library_construction_protocol: DNA MOLM-13 cells were pelleted by centrifugation at 500g for 5 min at 4 °C. All supernatant was removed carefully. Fifty microliters of transposase mixture (25μl of 2× TD buffer, 1μl of Tn5, 0.5μl of 1% digitonin, and 23.5μl of nuclease-free water) were added to the cells, and the pellet was disrupted by pipetting. Transposition reactions were incubated at 37 °C for 30 min with agitation at 300 rpm. Transposed DNA was purified using a QIAGEN MinElute Reaction Cleanup kit (28204), and purified DNA was eluted in 10 μl of elution buffer (10 mM Tris-HCl, pH 8).
attributes.tag: GEO Accession
attributes.value: GSM4407651