title: GSM2549746: H2O2 rep2; Staphylococcus aureus; RNA-Seq
library_construction_protocol: Harvested cells were lysed using Rususpension Buffer (10 mM Tris-HCl pH 7.5; 30 mM MgCl2; 30 mM NH4Cl) containing 500 µg/mL lysostaphin. RNA was extracted with the Qiagen RNeasy Mini kit and then treated with DNase I. Ribosomal depletion was performed using the Illumina Ribo-Zero rRNA Removal (Bacteria) kit. RNA libraries were prepared for sequencing using NEBNext Ultra Directional RNA Library Preparation kit for Illumina.
attributes.tag: GEO Accession
attributes.value: GSM2549746