title: GSM4314207: 1GV_10c_2; Mus musculus; RNA-Seq
library_construction_protocol: polyA RNAs: Each oocyte  was lysed in RLT-plus buffer. The oligo-dT beads were used to isolate the polyA RNA. The oocyte fractions were picked after oocyte lysis For polyA RNAs: Single oocyte RNA-seq libraries were prepared according to a published single-cell RNA seq pipeline with subtle modifications (Macaulay et al., 2016). Briefly, the oocytes at desired stages were collected individually into 2.5 ml RLT plus and stocked at -80 °C until all samples were collected. In the beginning of RNA purification, ERCC RNA spike-in mix (ThermoFisher 4456740) was diluted 10^5 fold, and 1 ml of the diluted ERCC mix was added to each lysis. Poly(A) RNA were isolated by oligo (dT) beads, reverse transcribed, amplified and purified. The purified cDNAs were analyzed by Bioanalyzer 2100 to confirm successful amplification range and quality. cDNAs passing a quality test were further constructed into sequencing libraries by Nextera DNA Sample Preparation Kit.
attributes.tag: GEO Accession
attributes.value: GSM4314207