title: Illumina HiSeq 2500 sequencing; Transcriptomic profiling of three different neuroblastoma cell lines (CLB-BAR, CLB-GE and SK-N-AS) in response to ALK inhibition
design: Transcriptomic profiling of three different neuroblastoma cell lines (CLB-BAR, CLB-GE and SK-N-AS) in response to ALK inhibition
library_construction_protocol: The CLB-BAR, CLB-GE and SK-N-AS cell lines were grown for 1hr and 24hrs in the absence (control conditions) or presence of crizotinib (250nM) or lorlatinib (30nM) Total RNA was isolated using the Promega Total RNA Isolation Kit. Random primed cDNA library preparation has been performed by GATC Biotech (Konstanz, Germany) using proprietary methods.
attributes.tag: Experimental Factor: compound
attributes.value: lorlatinib
attributes.tag: Experimental Factor: dose
attributes.value: 30
attributes.tag: Experimental Factor: time
attributes.value: 1
attributes.tag: Experimental Factor: cell line
attributes.value: CLB-BAR