title: GSM2112271: Control3; Sus scrofa; miRNA-Seq
library_construction_protocol: Total RNA was extracted from the longissimus dorsi muscle of the pigs using Total RNA Kit Ι reagent (Omega Bio-Tek, Norcross, GAUSA) according to the manufacturer protocol. DNase (Promega, Madison, WI, USA) was used to eliminate trace genomic DNA contamination. RNA purity was identified by determining the absorbance of the RNA at 260 nm and 280 nm [31]. The quality of the extracted RNA was examined using the 28S, 18S, and 5S rRNA bands on ethidium bromide-stained agarose gels. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2112271