title: GSM4158848: IbBBX24_ChIPSeq; Ipomoea batatas; ChIP-Seq
library_construction_protocol: The leaves of the OE-16 line infected with Fob at 1 dpi  were ground to a powder in liquid nitrogen and subjected to nuclear isolation   and protein-DNA complexes were isolated with IbBBX24 antibody. The leaves of the OE-16 line infected with Fob at 1 dpi were used for the ChIP assay. In brief, leaves (approximately 2 g) were cross-linked in 1% formaldehyde under a vacuum. The cross-linking was stopped with 0.125 M Gly. The sample was ground to a powder in liquid nitrogen and subjected to nuclear isolation. Anti-IbBBX24 (1:150 dilutions) antibodies were used to immunoprecipitate the protein-DNA complex, and the precipitated DNA was recovered. The precipitated DNA was sequenced using the Illumina HiSeq 2500 SE50 system according to the manufacturer  s instructions.
attributes.tag: GEO Accession
attributes.value: GSM4158848