title: GSM3731676: PWD_wt_DMC1 SSDS; Mus musculus; ChIP-Seq
library_construction_protocol: For DMC1 SSDS, testicular samples were obtained frozen and were directly thawed in 1% paraformaldehyde and gently dissociated. Genomic DNA for whole genome sequencing was extracted from the testicular samples before fixation with the DNeasy Blood and Tissue kit (Qiagen). DMC1 ChIP was performed as described previously (Smagulova et al., 2011; Khil et al., 2012; Brick et al., 2012) with minor modifications.  Sequencing libraries for anti-DMC1 were prepared following the method described in (Khil et al., 2012). Single Stranded DNA Sequencing (SSDS)
attributes.tag: GEO Accession
attributes.value: GSM3731676