title: GSM2805838: CS_FB3; Cynoglossus semilaevis; RNA-Seq
library_construction_protocol: To increase biological repeatability, every five tissues from males or females were firstly mixed as one sample, and three samples for each tissue were used for the following experiment. Thus, 24 samples named CSE_MB1-3, CSE_MP1-3, CSE_MG1-3, CSE_ML1-3, CSE_FB1-3, CSE_FP1-3, CSE_FG1-3, and CSE_FL1-3 were acquired and their total RNA was extracted via Trizol reagent (Invitrogen, USA) and measured with the Agilent 2100 RNA 6000 Nano kit (Agilent, USA) for quality control. Twenty-four cDNA libraries were constructed using 3 μg RNA (RIN > 7.0) from each sample by the conventional protocol, and the subsequent sequencing was carried with an Illumina HiseqTM 4000 by the Gene Denovo Biotechonolgy Co. (Guangzhou, China). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2805838