title: GSM3671361: KRAS, non-senescent, rep 2; Mus musculus; RNA-Seq
library_construction_protocol: For mRNA-seq we isolated tdTomato+SA-bGal– and tdTomato+SA-bGalhigh fractions from 3 mice. Total RNA from FACS-sorted cells was isolated by TRIzol (Invitrogen) extraction followed by Rneasy Plus Micro Kit (Qiagen), using >10,000 cells of each sample. Profiling was conducted using an adapted CEL-Seq2 protocol:  3' cDNA was synthesized and barcoded, followed by RNA synthesis, amplification by in vitro transcription and library generation for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3671361