title: GSM2112332: Betula luminifera miRNA; Betula luminifera; miRNA-Seq
library_construction_protocol: Total RNA was extracted from the samples using Trizol reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. Equal quantities of total RNA from roots, stems, and leaves were pooled to generate a sRNA library (LC Sciences, Hangzhou, China) using the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA). Approximately 20 ug of total RNA were used to prepare Degradome library. Approximately 150 ng of poly(A) RNA was used as input RNA and annealing with Biotinylated Random Primers
attributes.tag: GEO Accession
attributes.value: GSM2112332