title: GSM4408768: GNPs CHD8 CKO rep 3; Mus musculus; RNA-Seq
library_construction_protocol: GNPs were isolated from the cerebellum of mice at P7 by Percoll density gradient centrifugation. RNA was harvested using Trizol reagent.  NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) was used with 1 µg of total RNA for the construction of sequencing libraries. ChIP was subjected with antibodies to CHD8 or normal IgG. Purified DNA was used to prepare a library with the use of SMARTer ThruPLEX DNA-Seq kit and SMARTer DNA unique dual index kit.
attributes.tag: GEO Accession
attributes.value: GSM4408768