title: Modulation of sub-acute ruminal acidosis by active-dry yeast supplementation and its effect on rumen fungal and protozoal populations in liquid, solid, and epimural fractions.
design: The Internal Transcribed Spacer 1 region (ITS1) of fungi was amplified using the primers ITS5 (5ê- GGAAGTAAAAGTCGTAACAAGG-3ê) and ITS2 (5ê- GCTGCGTTCTTCATCGATGC-3ê) (White et al., 1990).