title: GSM2805225: lfy_flower_SYD; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: SYD-specific antiserum was raised in rabbits using a SYD-specific peptide (MKEERQRRIRERQKE-C, EZBiolab, Supplementary Figure S5A). Specificity of the antiserum was confirmed by Western blots on sonicated nuclei fractions using SYD antiserum (1:500) or H3 antibody (pAB-060-050, Diagenode, as a loading control), followed by probing with secondary anti-rabbit HRP (1:30 000). The detection was performed with CDP-star reagent (NEB #N7001).  Nuclei were sonicated during 15 min with a Covaris M220 (75W peak power/5% duty factor/200 cycles/burst) (Ikeuchi et al., 2015). Chromatin immuno-precipitation was performed as previously described (Engelhorn and Turck, 2017) and 12 µl of SYD-specific antiserum and 3µl of H3K27me3 (Diagenode, C15410069) were used per IP. ChIP DNA was amplified using Microplex Library Preparation kit v2 (Diagenode, C05010012).
attributes.tag: GEO Accession
attributes.value: GSM2805225