title: Illumina HiSeq 2000 paired end sequencing of SAMD00089592
library_construction_protocol: Lotus japonicus MG-20 inoculated with R. irregularis DAOM181602 were grown in a pot separated by 37 ????????????????????????????????????????????????m nylon mesh bag, whose pore size is smaller than the root but larger than the mycelium, at 25????????????????????????????????C for 8 weeks. The roots including IRM were sampled. RNA was extracted by using RNeasy Plant Mini Kit (Qiagen) with RNase-Free DNase Set (Qiagen). RNA-seq library were constructed by using TruSeq RNA Sample Prep kit. After the quality and quantity were confirmed by using Agilent 2100 Bioanalyzer and KAPA Library quantification kit, the libraries were sequenced by HiSeq 2000.