title: GSM3671019: vivo_P6_small_rep2; Mus musculus; RNA-Seq
library_construction_protocol: A directional RNA-seq library was prepared as described previously (Hamazaki et al. 2015). Briefly, poly(A)+ RNAs were extracted from the oocytes by using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). cDNA libraries were constructed from purified poly(A) RNA using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). cDNA libraries were amplified by 12-cycle PCR and checked for length distribution.
attributes.tag: GEO Accession
attributes.value: GSM3671019