title: GSM2459336: wild_type_K4_biorep2_techrep2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Yeast were spheroplasted and 50 ug of chromatin solution was used per immunoprecipitation. Chromatin solutions were pre-cleared using protein G agarose for 1 hr. at 4 degrees. Immunoprecipitations were performed overnight using Abcam ab1791 (histone H3) or ab8580 (H3Lys4 3me). Illumina barcodes were ligated, low cycle PCR was performed, and library quality was assessed using the Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM2459336