title: GSM4041651: FVB3-FF; Mus musculus; RNA-Seq
library_construction_protocol: The FVB/NJ mammary glands were placed in 10 ml of a digestion medium con-taining EpiCultTM-B Mouse Medium Kit (#05610, StemCell Technologies), Col-lagenase/Hyaluronidase (#07912, StemCell Technologies) and 1% penicillin streptomycin (Gibco). The mammary gland was digested overnight in a thermocycler maintained at 37 C with continuous rotation. RNA was isolated from the cell-lines and the mammary glands using the RNeasy Mini Kit (#74104, Qiagen) according to manufacturer protocol. scRNA-Seq libraries were prepared following the Single Cell 30 Reagent Kits v2 User Guide (Manual Part # CG00052 Rev A) using the following Single Cell 30 Reagent Kits v2: ChromiumTMSingle Cell 3' Library & Gel Bead Kit v2, PN-120237, Single Cell 3' Chip Kit v2 PN-120236, and i7 Multiplex Kit PN-120262 (10X Genomics). 10xBulk and Fresh frozen mRNA-Seq were made using total RNA and the Illu-mina TruSeq mRNA sample preparation kit. scRNA-Seq Libraries were run on an Illumina HiSeq 4000 as 2 150 paired-end reads. 10x bulk andfresh frozen mRNA-Seq Paired end (2 50bp) sequencing was performed on the Illumina HiSeq 2000/2500 sequencer
attributes.tag: GEO Accession
attributes.value: GSM4041651