title: GSM4259661: ATACseq_Sub8_ABD_rep2; Homo sapiens; ATAC-seq
library_construction_protocol: Fat biospsies were collected, adipocytes were isolated by centrifugation. Nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina).DNA was purified with MinElute PCR Purification Kit (Qiagen). Tagmented DNA was amplified with Kapa HiFi Hot Start Kit (Kapa Biosystems) using 16 PCR cycles. Amplified libraries were purified with Agencourt AMPure XP (Beckman Coulter). Fragment distribution of libraries was assessed with Agilent Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM4259661