title: GSM4661361: WT MEF cells - replicate 4; Mus musculus; RNA-Seq
library_construction_protocol: Strand specific, polyA-enriched RNA sequencing was performed as described earlier (Haack et al., 2013). Briefly, RNA was isolated from whole-cell lysates using the Total RNA kit (Peqlab, VWR) and RNA integrity number (RIN) was determined with the Agilent 2100 BioAnalyzer (RNA 6.000 Nano Kit, Agilent). For library preparation, 1 μg of RNA was poly(A) selected, fragmented, and reverse transcribed with the Elute, Prime, Fragment Mix (Illumina). A-tailing, adaptor ligation, and library enrichment were performed as described in the TruSeq Stranded mRNA Sample Prep Guide (Illumina). RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies). RNA libraries were sequenced as 150 bp paired-end runs on an Illumina HiSeq4000 platform.
attributes.tag: GEO Accession
attributes.value: GSM4661361