title: GSM1915113: H3K27Ac ChIP-Seq_Proliferating IMR90; Homo sapiens; ChIP-Seq
library_construction_protocol: Five to 30 million IMR90 cells were crosslinked for 10 minutes using 1 % formaldehyde, followed by quenching with 0.125M glycine for 10 minutes. To prepare chromatin from crosslinked-senescent cells, aliquots of less than 1 million cells were kept in individual eppendorf tubes for sonication to maximize the chromatin fragmentation efficiency. After purifying immunoprecipitated DNA, a TruSeq ChIP Sample Prep Kit (Illumina) was used to construct the ChIP-Seq library following  the manufacturer's protocol except for amplifying the adaptor-ligated library for 15 cycles. ChIP-Seq libraries were sequenced using an illumina HiSeq 2000 platform with single end reads of 50 bases. Four libraries at equal molarity were pooled to aim for a sequencing depth of 20-40 million aligned reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM1915113