title: Seasonally dynamic fungal communities in Quercus macrocarpa phyllosphere differ among urban and rural environments
design: Fungus-specific ITS1F and ITS4 primers were used to amplify the ITS region flanked by the 18S and 25S ribosomal RNA genes. the amplicons were directly 454-sequenced in reverse direction using a primer that incorporated the ITS4 and the 454-sequencing A primer.