title: GSM4478701: transposon mutant library subjected to carbon starvation in the absence of pyocyanin, replicate2; Pseudomonas aeruginosa; OTHER
library_construction_protocol: Genomic DNA (gDNA) was extracted from the pelleted samples using the DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer's directions. All the steps for sequencing library preparation followed exactly the protocol used by Basta et al. (mBio, 2017), including (i) DNA shearing by sonication (to produce 200-500 bp fragments), (ii) end-repair, (iii) addition of poly(C) tail and (iv) enrichment of transposon-genome junctions and addition of adapter for Illumina sequencing by PCR
attributes.tag: GEO Accession
attributes.value: GSM4478701