title: GSM2982034: RES lamb 2 [H06]; Ovis aries; RNA-Seq
library_construction_protocol: Liver samples stabilized in RNAlater were homogenized using RLT (Qiagen), Lysing Matrix D and a FastPrep-24 instrument (MP Biochemicals); RNA purification was performed with the  RNeasy Mini kit and RNase-Free DNase Set kit (Qiagen). Poly(A)+ mRNA fraction was isolated from total RNA and cDNA libraries were obtained following Illumina´s recommendations. Briefly, poly(A)+ RNA was isolated on poly-T oligo-attached magnetic beads and chemically fragmented prior to reverse transcription and cDNA generation. The cDNA fragments then go through an end repair process, the addition  of a single 'A' base to the 3' end and then ligation of the adapters. Finally, the products are purified and enriched with PCR to create the indexed final double stranded cDNA library. The quality of the libraries was analyzed in Bioanalyzer 2100, High Sensitivity assay; the quantity of the libraries was determined by real-time PCR in LightCycler 480 (Roche). The pool of the libraries was sequenced by paired-end sequencing (100 × 2) in an Illumina HiSeq 2500 instrument.
attributes.tag: GEO Accession
attributes.value: GSM2982034