title: GSM4260245: FurKO_Minus_3hr_Rep1; Neisseria gonorrhoeae F62; RNA-Seq
library_construction_protocol: RNA was extracted with TRIzol RNA was depleted of rRNA using the Agilent Microbe Express kit.  Libraries were made using the BioChain High Yield Directional mRNA Sample Prep kit per the manufacturer's instructions. cDNA libraries were subject to electrophoresis on an 8% Tris-borate-EDTA (TBE) gel for 60 min at 140 V. cDNA fragments between 200 and 500 bp were extracted from the gel in Tris-EDTA (TE) buffer with agitation, and libraries were analyzed on an Agilent Technologies 2100 Bioanalyzer to confirm cDNA library size and quantity. Libraries were sequenced using 72-bp single-end reads on an Illumina GAIIX machine
attributes.tag: GEO Accession
attributes.value: GSM4260245