title: GSM2266539: BFR2_X13-1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was isolated from liver tissue using TRIzol reagent (Invitrogen). Samples of RNA isolated from testis samples with integrity values > 9 were used for library preparation. RS-122-2101-TruSeq® Stranded mRNA LT – SetA kit (Illumina, San-Diego, CA) was used to isolate intact poly(A)+ RNA from 4 µg of total RNA and construct strand-specific libraries with multiplexing indexes. The quality and purity of the libraries was assessed by The Agilent 2200 TapeStation system,  and the concentration of the libraries was measured by real time PCR with primers for P5 and P7 flow cell oligo sequences using KAPA Library Quantification Kit (KR0405, Kapa Biosystems, Boston, MA) in a 384-well plate on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA).
attributes.tag: GEO Accession
attributes.value: GSM2266539