title: GSM2109489: Sample_8-Lymphnode-Tcell-WT4; Mus musculus; OTHER
library_construction_protocol: Cells were lysed in 100 μl of mild detergent (100mM Tris-HCL pH 7.4, 10mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) on ice for 5 minutes to release the nuclei.  Pelleted nuclei were then incubated for 90 minutes with Tagment DNA Enzyme transposase from the Nextera DNA Library Preparation kit (Illumina).  The reaction was stopped and DNA was purified using the MinElute Reaction Cleanup kit (Qiagen) The eluted DNA was amplified with the NEBNext High-Fidelity 2x PCR Master Mix using Nextera index primers (Illumina).  The indexed libraries were purified using the MinElute PCR Reaction Cleanup (Qiagen) before sequencing on an Illumina HiSeq 2500 with 25 bp paired-end reads.
attributes.tag: GEO Accession
attributes.value: GSM2109489