title: GSM1915256: L20-1 (juvenile); Gallus gallus; miRNA-Seq
library_construction_protocol: All the chickens were raised in cages under the same environment with ad libitum water and food. Healthy individuals were randomly sampled and then slaughtered at juvenile of 20 weeks (20w group) and egg-laying stages of 30 weeks (30w group). The liver tissue was immediately collected, snap-frozen in liquid nitrogen, and stored under -80°C. The small RNA used for sequencing was extracted with mirVana™ miRNA Isolation Kit (Austin TX, US) according to the manufacturer’s instruction. The quality and quantity of the total RNA were assessed by the Agilent 2100 Briefly, 3’ and 5’ RNA adapters were ligated to the total RNA with T4 RNA ligase, successively. Subsequently, the ligated RNAs were subjected to reverse-transcriped PCR to synthesis cDNA, and then the cDNA was amplified by PCR. The amplification products with appropriate length were purified from agarose gel to construct sequence libraries, and then were sequenced with a single-read 1×36 nt multiplex procedure on an Illumina HiSeq 2500 following the manufacturer’s instruction (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM1915256