title: Illumina of sludge samples: Anaerobic phenol biodegradation
design: 16S NGS analysis was performed at Stab Vida Lda (Lisbon, Portugal). Sequencing targeted the V5 and V6 regions (250bp) of the 16S rRNA gene using the primers 799F (5-AACMGGATTAGATACCCKG-3) and 1175R1 (5-ACGTCRTCCCCDCCTTCCT-3) (Bonder et al. 2012). Samples were then sequenced with MiSeq Reagent Kit Nano though the Illumina MiSeq platform.