title: GSM4483270: mRNA_library; Homo sapiens; RNA-Seq
library_construction_protocol: A total of 20×106 Jurkat cells were stimulated by ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL) in RPMI with 10%FBS at the concentration of 5×106/ mL. The activator was added at the dose of 25μl/ mL. After 24h of stimulation, cells were harvested and stained for surface CD69 (FN50, Biolegend), and 2×106 CD69- (Bottom 25%) cell population were sorted using FACS (Fusion, BD) for single cell sequencing. The mRNA library was prepared following to the Chromium User Guide.
attributes.tag: GEO Accession
attributes.value: GSM4483270