title: GSM4041658: 10-day-4T1; Mus musculus; miRNA-Seq
library_construction_protocol: 500ul of blood was collected from live mice under isofluorene anesthetic, collected with 40ul of the anticoagulant and spun down for 10mins @ 2000 RPM at 4°C to collect the plasma Libraries were prepared as per manufacturer using Illumina's TruSeq smallRNA kit with 15 cycles of PCR amplification performed.  Desired product size of libraries (~146bp) were isolated using Sage Science's Blue Pippin machine on a 3% agarose gel and confirmed on Agilent's Bioanalyzer using a High Sensitivity DNA chip, followed by sequencing on Illumina's MiSeq using the MiSeq v3 reagent kit
attributes.tag: GEO Accession
attributes.value: GSM4041658