title: Illumina HiSeq 2500 sequencing; Liver Transcriptome Response to Hyperthermia Stress in Three Distinct Chicken Lines
design: Liver Transcriptome Response to Hyperthermia Stress in Three Distinct Chicken Lines
library_construction_protocol: At 17 days of age, birds were transferred to temperature-controlled chambers and acclimated to the new environment for five days. From days 22 to 28 of age, the heat stress group (n=24) were exposed to high heat 35°C for 7 h per day and remained at 25°C at all other times. There was also a contemporary control group (n=24) that was maintained at normal temperature of 25°C. The liver samples were collected at 3 h after heat treatment.  The acute heat treatment group (n=12) were collected on 1d, and the chronic heat treatment group (n=12) were collected on 7d after 7 days of cyclic heat. All animal care and experimental procedures were reviewed and approved by the Animal Care and Use Committee (#11-07-6460-G) of Iowa State University. illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA)
attributes.tag: Experimental Factor: Broiler line
attributes.value: strain
attributes.tag: Experimental Factor: Chronic Control
attributes.value: stimulus
attributes.tag: Experimental Factor: 4
attributes.value: age