title: fungal metagenome of plant root
design: Library prep was completed using a dual-indexing protocol. For bacteria, we sequenced the V4 hypervariable region of the 16S rRNA gene, using the 515-F / 806-R primer pair. For fungi, we sequenced the ITS1 region of the rRNA gene using the ITS1-F / ITS2 primer pair. Primers included Illumina adapters and barcodes to enable multiplexing of samples.