title: Maxxa WashU cDNA
design: RNA was provided to Washington University in St. Louis to develop 454 EST sequences on the FLX platform.  cDNA was poly-T primed and amplification adaptors included modified SMRT sequences that allowed for trimming of the poly-A (or -T) sequences prior to sequencing based on an MmeI cut site.  Once the cDNA was SMRT amplified, the library was normalized using Evrogen's Trimmer kit (DSN).  A sample (0.5 FLX plate) was sequenced using FLX technology (prior to Titanium) and the raw reads contain some adaptor sequences.  The bead library was then sent to BYU on dry-ice were 2 additional 0.5 plates were sequenced from the same 454 bead library (same titer was used). The raw 454 reads contain adaptor sequences. The three runs of Maxxa are FJEO9QG02.sff,  FJEO9QG01.sff, and   FEV57AF02.sff