title: 24335 tumor RNA
design: RNA was extracted from live cultured 24335 cells using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). rRNA removal was performed using the Ribo-Zero Kit (Epicentre) following the manufacturers instructions.  The library was prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturers instructions and sequenced at the Partners HealthCare Personalized Medicine/ Genetics and Genomics core facility (personalizedmedicine.partners.org) using HiSeq2500 (Illumina).