title: Single cell RNA-seq of human pilocytic astrocytoma: Sample BT801_Minus_PL1_E10_BP3411_S159
design: Single cell Libraries were prepared using the SmartSeq2 Ultra Low Input v4 Kit (Takara) according to the manufacturer's instructions, with the following modifications. An oligonucleotide specific to a sequence contained in exon 18 of BRAF was added in equal stochiometry along with the Smart CDS Primer IIA. Sequencing libraries from samples with more than 0.3 ng of cDNA greater than 400 bp were prepared using a Nextera XT reaction.  Libraries were pooled and sequenced with paired end reads using a high output kit on an Illumina NextSeq500.