title: GSM4158389: MCF10A_RNA_WT_1 [cont_rep1]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using RNA purification kit (GeneAll) according to the manufacturer's instructions.  RNA quality was measured on a Bioanalyzer (Agilent) and only RNA with RIN score >9 was used for library preparation. Messenger RNA (mRNA) was enriched from 1 μg of total RNA by Poly(A)  mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer's instructions. cDNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer's protocol. Library concentration was measured by DNA High Sensitivity Kit (Invitrogen) on a Qubit fluorometer (Invitrogen). Library quality and fragment sizes were assessed on a Bioanalyzer (Agilent) or on a Tape station (Agilent). RNA-Seq libraries from at least two biological replicas for each condition were sequenced on Illumina Hi-seq 2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM4158389