title: lung microbiome from sputum for 111B_224_2.11_PostTherapy
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode TACGAGTATG for subject 224 at a PostTherapy visit on 07_30_10