title: GSM3138067: D1_3; Trichoderma asperellum; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer's instruction. The mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. After agarose gel electrophoresis, the suitable fragments are selected for the PCR amplification as templates.
attributes.tag: GEO Accession
attributes.value: GSM3138067