title: GSM3730742: L1 larva; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Animals of different stages, i.e., embryo, L1 larva and young adult, were collected and total RNAs were extracted using TRIzol (Invitrogen) following the manufacturer's instructions. Approximately 100 µg total RNAs were extracted for each sample. Around 900 ng of poly-A tailed mRNAs was purified using Dynabeads™ mRNA Purification Kit (Invitrogen) based on the user's manual for each library preparation. Nanopore sequencing libraries were constructed using Direct RNA sequencing kit (cat# SQK-RNA001). The libraries were loaded onto Nanopore R9.4.1 flow cell (cat# FLO-MIN106) and sequenced on MinION acquired from Oxford Nanopore Technologies. The software used for sequencing was MINKNOW 2.1 with base-caller, Albacore (v2.0.1).
attributes.tag: GEO Accession
attributes.value: GSM3730742