title: GSM3136860: RNAseq in EB+0hr(iNgn2.Isl1.Lhx3.V5) replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA-sseq: Cells were collected 0, 12 or 48 hours after Doxycycline TF induction and RNA was isolated by resuspending in Trizol reagent (Invitrogen, 15596026) followed by purification using Qiagen RNeasy mini kit (Qiagen, 74106). Illumina TruSeq LS kit v2 (RS-122-2001; RS-122-2002) was used to prepare RNAseq libraries. The final quantification of the library before pooling was done with KAPA library amplification kit (Roche Lightcycler 480).
attributes.tag: GEO Accession
attributes.value: GSM3136860