title: CH Campala Lr22a 2D chromosome shogun library raw reads
design: For shotgun sequencing, DNA of chromosome 2D was amplified by multiple displacement amplification (MDA) as described previously36. In total, 30,000 copies of chromosome 2D were flow-sorted from each line. The purity of the sorted fraction was 94%. The chromosomes were treated with proteinase K and the purified DNA was amplified using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, UK). Three independent MDA products from each sorted chromosome fraction were pooled into one sample to reduce amplification bias.