title: GSM3136949: Ca_3_30; Candida tropicalis; Homo sapiens; RNA-Seq
library_construction_protocol: To isolate human RNA aliquots of infected or mock infected samples were added to a PAXgene® Blood RNA Tube (PreAnalytiX) and processed with the PAXgene® Blood RNA Kit (PreAnalytiX) according to the manufacturer's protocol. For fungal RNA isolation aliquots were added to ice-cold water, centrifuged and immediately frozen in liquid nitrogen. The cell pellet was further processed with the RiboPure™-Yeast Kit (Thermo Fisher Scientific) according to the manufacturer's protocol. RNA quantity was determined with NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific) and RNA quality was verified with an Agilent 2100 Bioanalyzer (Agilent Technologies). Fungal and human RNA samples were pooled subsequently in a quantitative ratio of 1:10. Library Preperation was performed by GATC Biotech using validated  standard procedures modified to optimize the method for automated  Preperation. Library Preparation consisted of the following steps:  Purification of poly-A containing mRNA molecules, mRNA fragmentation, Random primed cDNA synthesis (strand specific), Adapter ligation and adapter specific PCR amplification
attributes.tag: GEO Accession
attributes.value: GSM3136949