title: GSM4408270: Sample 7_RRBS_high_cortisol; Bos taurus; Bisulfite-Seq
library_construction_protocol: DNA was extracted from CD4+ cells following the QIAamp DNA Blood Midi Kit (Qiagen) procedures. Libraries were prepared using the NEB Next Ultra DNA library preparation kit for Illumina and methylated adapters. Subsequently, size‐selected (150-175 bp and 175-225 bp) and adapter ligated fragments were bisulfite converted using the EZ DNA Methylation Gold kit (Zymo Research). Fourteen cycles of PCR were performed and the products were purified using AMPureXP beads. Reduced Representation Bisulfite Sequencing (RRBS) libraries were used for cluster generation and subsequent sequencing on Illumina HiSeq2500 PE 2x50bp (NXT-Dx Ghent, Belgium, http://www.nxt-dx.com/)
attributes.tag: GEO Accession
attributes.value: GSM4408270