title: GSM4106462: Pik3ca+/H1047R;Trp53-null tumor BHV138; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from frozen from Pik3ca*;Trp53-null (BHV ) and Pten;Trp53-null (BIF) mice  mouse malignant mesothelioma tumors (n=6 each)  using GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). DNA was eliminated using the DNA-free kit (Ambion-Life Technologies). 2 µg of total RNA samples was used as provided by the user. RNA Integrity Numbers were in the range 7.5 to 8.9 (average 8.5) when assayed on an Agilent 2100 Bioanalyzer. PolyA+ RNA fraction was extracted and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq RNA Sample Preparation Guide" (Part # 15008136 Rev. A). Adapter-ligated library was completed by limited-cycle PCR with Illumina PE primers (8 cycles). The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx with SBS TruSeq v5 reagents by following manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4106462