title: DNA-Seq of Pseudodebis zimri: adult gut, CR421
design: DNA was extracted with the Qiagen Blood and Tissue kit. The V4 hypervariable region of the bacterial 16s rRNA was amplified with primer set 515f/806r. For some samples, part of the fungal ITS gene was also amplified with primer set ITS1f/ITS2. Amplicons were sequenced on an Illumina MiSeq platform.