title: NextSeq 500 sequencing; RNA-seq of equine and canine mammary stem cells treated with DMBA
design: RNA-seq of equine and canine mammary stem cells treated with DMBA
library_construction_protocol: Cells were cultured for 24 h prior to a 4 h treatment with vehicle (DMSO) or DMBA. Total RNA was extracted with TRIzol reagent following the recommendations of the manufacturer. The quality of total RNA was evaluated using the Agilent 2100 bioanalyzer (Agilent, Palo Alto, CA, USA) with the RNA 6000 Nano LabChip kit.  RNA-seq libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) using 500 ng total RNA followed by polyA+ enrichment.
attributes.tag: Experimental Factor: organism
attributes.value: Canis lupus familiaris
attributes.tag: Experimental Factor: compound
attributes.value: 7,12-dimethylbenz(a)anthracene
attributes.tag: Experimental Factor: dose
attributes.value: 5