title: GSM2108615: 72H_M1; Homo sapiens; RNA-Seq
library_construction_protocol: HIECs were trypsinized and spun down, then lysed and RNA was extracted using Norgen Total RNA isolation kits as per the manufacturer's instructions. RNA quality was assessed using Agilent RNA Nano 6000 kit (Agilent Technologies, Inc, Santa Clara, CA) run on a Bioanalyzer 2100 (Agilent). All samples had high integrity with RINs above 9.2 (with an average of 9.7). Samples were submitted to the UNC High Throughput Sequencing Facility (HTSF) for TruSeq Stranded Total RNA library preparation (Illumina, San Diego, CA) and paired-end 50 bp sequencing on a HiSeq 2000 (Illumina) multiplexing 6 samples per lane.
attributes.tag: GEO Accession
attributes.value: GSM2108615