title: GSM2728638: Aged rep5; Homo sapiens; RNA-Seq
library_construction_protocol: Biopsies were timed between 5-13 days post-LH surge according to home ovulation tests.  Endometrial biopsies were obtained using a Wallach Endocell® endometrial sampler.  The device was introduced into the uterus until resistance from the fundus was felt. The piston was withdrawn to generate negative pressure and the device rotated through 360° as it was gradually withdrawn from the fundus to the cervical ostium. Total RNA was extracted using the Allprep DNA/RNA Mini kit (Qiagen 80204). mRNA was isolated from total DNA-free RNA (350ng) using the Dynabeads mRNA purification kit (Life Technologies 61006). Indexed, strand specific libraries were prepared using the ScriptSeq v2 RNA-Seq Library Preparation kit (Epicentre SSV21106) according to the manufacturer's instructions. Libraries were quantified/assessed using both the KAPA Library Quantification Kit (KAPA Biosystems KK4824) and Bioanalyzer 2100 system (Agilent). Indexed libraries were pooled and sequenced with a 100bp single-end protocol on an Illumina HiSeq2500 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM2728638