title: Illumina MiSeq sequencing; qiita_ptid_5704:10246.PP.B6
design: We collected soil samples along two arctic transects in North America, NAAT and Eurasia, EAT. Each transect extends across all five bioclimatic subzones of the Arctic.
library_construction_protocol: Illumina EMP protocol 515fbc, 806r amplification of 16S rRNA V4
attributes.tag: barcode
attributes.value: CCTAACGGTCCA
attributes.tag: center_name
attributes.value: UCSDMI
attributes.tag: center_project_name
attributes.value: EMP-Timling
attributes.tag: extraction_robot
attributes.value: NA
attributes.tag: extractionkit_lot
attributes.value: NA
attributes.tag: linker
attributes.value: GT
attributes.tag: mastermix_lot
attributes.value: 620338
attributes.tag: orig_name
attributes.value: PP.B6
attributes.tag: pcr_primers
attributes.value: FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT
attributes.tag: plating
attributes.value: NA
attributes.tag: primer
attributes.value: GTGTGYCAGCMGCCGCGGTAA
attributes.tag: primer_date
attributes.value: 73118
attributes.tag: primer_plate
attributes.value: 1
attributes.tag: processing_robot
attributes.value: ROBE
attributes.tag: project_name
attributes.value: Arctic Transects Soils
attributes.tag: run_center
attributes.value: UCSDMI
attributes.tag: run_date
attributes.value: 9/26/18
attributes.tag: run_prefix
attributes.value: Arctic_Transects_Soils_1-3_Prather_Seawater_SU18_1_S1_L001
attributes.tag: runid
attributes.value: 180926_M05314_0126_000000000-C4PNL
attributes.tag: sample_plate
attributes.value: Arctic_Transects_Soils_Plate_1
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: tm1000_8_tool
attributes.value: NA
attributes.tag: tm300_8_tool
attributes.value: 208484Z
attributes.tag: tm50_8_tool
attributes.value: 108364Z
attributes.tag: water_lot
attributes.value: RNBG7049
attributes.tag: well_description
attributes.value: Arctic_Transects_Soils_Plate_1_PP.B6_A4
attributes.tag: well_id
attributes.value: A4