title: GSM3672136: Extracted RNA 4-day old chicken  anterior slice #1 from dorsal-to-ventral axis; Gallus gallus; RNA-Seq
library_construction_protocol: Total RNA was isolated from individual cryosection using TRIzol reagent (Ambion), according to the manufacturer's manual. RNA extraction, amplification and sequencing libraries were performed as described in {Junker et al., 2014}. Briefly, RNA samples were treated according to the CEL-seq protocol {Hashimshony, 2012 #36}. Illumina sequencing libraries were prepared with the TruSeq small RNA sample prep kit (Illumina). Libraries were sequenced on an Illumina HiSeq2500 using 50bp paired end sequencing and Illumina Illumina NextSeq 500 using 75bp paired end sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3672136