title: GSM3137666: LCM-3; Mus musculus; RNA-Seq
library_construction_protocol: For FACS isolated replicates, DRG from E14.5 embryos were harvested and dissociated with Trypsin-EDTA, resuspended in HBSS, and isolated using Diva or Aria TMII cell sorter. For LCM isolated replicates, spinal cords from E14.5 embryos were harvested and flash frozen on dry ice, stained with hematoxylin, dessicated, and microdissected with a Zeiss PALM system. All RNA was extracted using GeneJet RNA Purification Kit (K0731, Fermentas). RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM3137666