title: GSM2982741: CD4+ T Cells Pool1 Spleen; Mus musculus; RNA-Seq
library_construction_protocol: Virus-specific TCR-transgenic T cells were given to 10-20 mice followed by infection to generate memory cells. At day 50 post-infection, memory cells in the spleen or adipose tissue were identified by surface stained and FACS-sorted to ~98% purity by a FACSAria-II machine at the UNC FACS core. RNA was extracted from ~10^5 sorted cells (pooled from 5-10 mice each in 2 independent replicates; Table S1), and purified on oligo(dT) dynabeads cDNA libraries were constructed using TruSeq Adapters
attributes.tag: GEO Accession
attributes.value: GSM2982741