title: GSM4483330: 32dscat_BM_MEP; Mus musculus; RNA-Seq
library_construction_protocol: Stem and myeloid (SMP) progenitor cells (Lin-Sca-Kit+CD34+, CD16_32lo) cells from spleen and bone marrow were sorted as described by Bumm et al., Cancer research 66, 11156-11165, 2006. Megakaryocyte-erythroid progenitors (MEP) were isolated as described in Challen et al., Cytometry A 75, 14-24, 2009. Total RNA was prepared using the PureLink RNA Mini Kit (Invitrogen). Libraries were checked for quality and concentration using the D5000 ScreenTape assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturer's instructions RNA-seq libraries prepared using the KAPA mRNA HyperPrep Kit (KAPA Biosystems) according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4483330