title: GSM2723896: upf1-delta_rep1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: After collection, RNA are extracted by hot acid phenol/chloroform method, precipitate with Ammonium acetate and ethanol. Samples were treated with DNaseI. Samples were treated by ribozero and libraries were prepared using TruSeq Stranded mRNA LT Kit. We strictly followed the protocol except that we started directly at the fragmentation step after the ribozero and we adjusted the number of cycle required to amplified libraries.
attributes.tag: GEO Accession
attributes.value: GSM2723896