title: GSM1904498: HFF_InfectedHSV2_8h; Homo sapiens; RNA-Seq
library_construction_protocol: All HCMV and HSV-2 materials were collected at the time of harvest by trypsinization and centrifugation, and snap-frozen prior to RNA extraction. HIV-1 materials were harvested directly into TRIzol reagent. TRIzol RNA extractions of RNA were performed according to the manufacturer’s instructions (Invitrogen/Life Technologies). All HCMV and HIV-1 sample conditions were subjected to strand-specific RNA-seq using the dUTP method (Parkhomchuk et al., 2009) with adaptations as described previously (Huelga et al., 2012). HSV-2 libraries were prepared with Illumina TruSeq Stranded mRNA Sample Preparation reagents. Strand-specific RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM1904498