title: GSM1915238: Sample 25: female whole-body x10; Nasonia vitripennis; RNA-Seq
library_construction_protocol: Focal females were pooled into groups of 10 and RNA was extracted from the whole bodies of these 10 individuals using the TRIzol Plus RNA purification system (Life Technologies, Paisley, UK). Total RNA samples were prepared for sequencing using the illumina TruSeq RNA Sample Prep Kit (illumina, San Diego, CA, USA) following the illumina TruSeq Sample Preparation v2 (Low Sample) protocol. Briefly, mRNA molecules were purified from total RNA samples using oligo-dT attached magnetic beads and fragmented using divalent cations at 94 °C. First strand cDNA synthesis was carried out using reverse transcriptase and random hexamer primers. Second strand synthesis was carried out using RNA polymerase I and RNase H. Overhangs resulting from fragmentation were converted to blunt ends and 3’ ends were subsequently adenylated. Sanger indexing adapters were ligated to the fragments which were then purified and PCR-amplified to create the final cDNA libraries for sequencing. Libraries were sequenced on the HiSeq2000 according to the manufacturer’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM1915238