title: GSM3211539: 163C1; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Cell suspensions of tissue sections of bladder CTC PDX models were obtained from CTC-PDX tumors by mechanically triturating the sample with a sterile scalper and forceps, without enzymatic digestion. RNA was isolated using the RNeasy Mini kit (Qiagen) in accordance with manufacturer's instructions. High-quality total RNA samples (RNA Integrity score >7.7 by Agilent Bioanalyzer) were subjected to poly A-selected sequencing library preparation using TruSeq RNA Sample Prep Kit ver.2 (Illumina) following manufacturer's protocol. The libraries were sequenced by HiSeq 2500 genome sequencer (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3211539