title: GSM2978418: RNASeq_DMSO_4h_rep; Homo sapiens; RNA-Seq
library_construction_protocol: After treatment with CBX7 inhibitor or DMSO control, RNA was harvested from purified 625,000 CD4+ T lymphocytes for each independent experiment with QIAzol (Qiagen) and chloroform extracted. The Smart-seq2 RNA-seq protocol was used for generation of cDNA, which was fragmented with Diagenode sonication (performed at 4 °C, 25 second pulse followed by 30 second recovery, for 2 x 10 cycles).
attributes.tag: GEO Accession
attributes.value: GSM2978418