title: Genome sequencing of Vitis vinifera sylvestris: Male
design: Plant was selected from INIAV, Dois Portos (Lisbon district, Portugal, 39.041395, -9.181956). Fresh leaves were collected. Leaves were grinded with mortar and pestle in liquid nitrogen and the DNA was purified with the DNeasy Plant Mini Kit (Qiagen), according to the manufacturers instructions. DNA quantity and quality was evaluated using a microplate reader Synergy HT (Biotek, Germany), using the software Gen5 (Biotek, Germany) and confirmed on a 1.7% (w/v) agarose gel.