title: GSM3673653: TET2-KO (CR2) RNA-seq Rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: DNA was extracted from cell pellets using the QIAamp DNA Mini Kit (Qiagen, catalog #51306) following the manufacturer's protocol. Whole RNA was extracted via TRIzol method as per manufacturer's protocol (Thermo Fisher, catalog #15596026), and was cleaned up using the RNeasy Mini Kit (Qiagen, catalog #74104). For methylation analysis, enriched DNA was submitted in duplicate along with unenriched input controls for library preparation (NEBNext® ChIP-seq Library Prep Reagent Set for Illumina) and high-throughput sequencing using the HiSeq 2500 (Illumina, San Diego, California) at The Centre for Applied Genomics. Each library generated approximately 75 million paired-end reads. RNA library preparation for each of the above cell lines was performed following the NEB NEBNext Ultra Directional Library Preparation protocol (poly-A mRNA) on 200ng total RNA. Libraries were sequenced in duplicate using the HiSeq 2500 at The Centre for Applied Genomics, with each library generating approximately 30 million paired-end reads.
attributes.tag: GEO Accession
attributes.value: GSM3673653