title: lung microbiome from sputum for 75A_178_4_Stable
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode TCTACGTAGC for subject 178 at a Stable visit on 04_20_10