title: GSM4158676: PM_3; Mus musculus; RNA-Seq
library_construction_protocol: In this study, the total RNA of each sample was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Briefly, 1% agarose gels were used to monitor RNA degradation and contamination. RNA purity was checked using the NanoPhotometer spectrophotometer (Implen). RNA concentration was measured using the Qubit RNA Assay Kit in the Qubit 2.0 Fluorometer (Life Technologies). RNA integrity was evaluated by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4158676