title: GSM2460326: wild type RGC 1; Mus musculus; RNA-Seq
library_construction_protocol: Retinas were removed, dissociated with papain digestion system (worthington Biochem. Corporation. #LK003150) according to protocol. For separation of RGCs from non-RGCs, we continue with cell separation using magnetic microbeads labelled with anti-CD90-antibody (MACS Miltenyi Biotec, #130-094-523; 130-041-305). After centrifugation, cell pellet was used for RNA-Extraction using Qiagen RNEasy Mini Kit (Qiagen, #74104). 1ug of total RNA was used for library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2460326