title: RNA-Seq of Lacidophilus: Generation 100 in SVF
design: Cells were grown to mid-log phase in SVF media at 37C anaerobic conditions, 100 Generations. Cells were pelleted and flash frozen. RNA was extracted with Zymo Direct-zol RNA miniprep kit, treated with Tubro DNase I and column purified. Bioanalyzer and sent to UIUC for sequencing.