title: Comparative chickpea leaf wound transcriptome
design: This data represents 454 RNA sequencing information from chickpea (Cicer arietinum variety; P-362) leaves before and after mechanical wounding. Leaves of eight week old plants were rapidly wounded with forceps to cover the entire leaf area. Leaves were kept for 5 minutes or 20 minutes, frozen in liquid nitrogen and RNA isolated. cDNA was prepared from these samples as well as those from unwounded samples (0 min). Sequencing was performed on a GS FLX pyrosequencer (Roche Life Sciences, USA). The total data output of sequencing run yielded 53.6 Mb, 34.4 Mb and 17.9 Mb for the unwounded control (C), 5 minute wounded and 20 minute wounded sample respectively, corresponding to a total 106 Mb data. The contigs produced by assembler were 4388 (C), 2886 (5 min), and 1683 (20 min) in number with average read lengths of 526 (C), 482 (5 min) and 470 (20 min).