title: GSM4157071: Sample_9_C1B; Homo sapiens; RNA-Seq
library_construction_protocol: Briefly, 3mm2 pieces of fresh-frozen brain tissue were placed in homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris Buffer pH 8.0, 1 mM DTT, 1X protease inhibitor, 0.4 U ul-1 RNaseIN, 0.2 U ul-1 Superasin, 0.1% v/v Triton X-100) for 10 minutes prior to mechanical disruption with a dounce homogenizer.  Homogenate was filtered through a 35 mM cell strainer before concentrating nuclei by centrifugation 400 g 5 minutes.  Nuclei were resuspended in FACS buffer (1 XPBS, 1 RNase-Free BSA, 0.2 U μl−1 of RNasin Plus RNase inhibitor, 10 ng ml-1 Hoechst 33342) prior to FACS to obtain a population of nuclei free cellular debris.Nuclei were washed once in PBS and resuspended in 0.04% BSA in PBS for loading on the 10x Chromium 3' chip. Cell capturing  and library preparation was carried as per kit instructions (Chromium Single Cell Kit [v2 chemistry])
attributes.tag: GEO Accession
attributes.value: GSM4157071