title: Streptococcus pneumoniae Genome sequencing and assembly
design: Briefly, your gDNA samples were fragmented using an enzyme based approach (part of NEBs NEBNext Ultra II kit work flow). Resulting fragments were purified (size selected), end-repaired and an Illumina specific adapter sequence was ligated to all fragments. Following quantification, the samples were individually indexed and a second size selection step was performed, using AMPure XP Beads. The libraries were quality controlled on a DNA chip (Agilent 2100 Bioanalyzer) and then sequenced on Illuminas MiSeq platform, using a MiSeq v3 (600 cycle) kit according to the manufacturers protocol.