title: GSM4260816: vy4_cells_control5; Mus musculus; RNA-Seq
library_construction_protocol: Clontech NucleoSpin® RNA XS kit was used to extract RNA RNAseq was performed on total RNA by normalizing the input mass to cell number according to cell type. For TCRδ+Vγ4+TCRβ– cells, input was normalized to 30,000 cells where possible (2 samples fell below this input target, reaching totals of 12,687 and 21,772 cells). For TCRδ+Vγ4+TCRβ+ cells, input was normalized to 5,000 cells where possible (3 samples fell below this input target, reaching totals of 2,036, 3,649, and 4,577 cells). cDNA was amplified using a SMARTer Ultra Low Input RNA Kit for Illumina Sequencing (Clontech) and sequenced at a target depth of 30 x 106 single-end (1 x 50 bp) reads per sample using a HiSeq 3000 System (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4260816