title: GSM2881002: W1_ACACGA_L007_R1_001; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA 88 was isolated using AMBION RNAeasy isolation kits.The quality of RNA was examined using a BioAnalyzer. A 1:1 ratio of Agencourt AMPure XP beads (Beckman Coulter Inc., Brea, CA) were used to purify resulting cDNA, and 1 ng cDNA was subsequently used to prepare a sequencing library with the Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA). Both whole-transcriptome amplification and sequencing library preparations were performed in a 96-well format to reduce assay-to-assay variability. Quality control steps were included to determine total RNA quality and quantity, the optimal number of PCR pre-amplification cycles, and fragment size selection. RNAseq libraries were sequenced using Illumina HiSeq 2500 according to the standard protocol.
attributes.tag: GEO Accession
attributes.value: GSM2881002