title: GSM4407796: 35955; Peromyscus maniculatus; RNA-Seq
library_construction_protocol: Animals were sacrificed using isoflurane as an anesthetic followed by cervical dislocation, and the livers were collected. RNAs were extracted with a Qiagen RNeasy Plus Mini kit as per manufacturer's recommendations (Qiagen, Valencia, CA) RNA integrity was assessed using the Agilent Bioanalyzer and samples had a quality score ≥ 8.6. RNA libraries were prepared using established protocol with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Lynn, MA). Each library was made with one of the TruSeq barcode index sequences and pooled together into one sample to be sequenced on the HiSeq 2x150bp pair-ended sequencing platform (Genewiz).
attributes.tag: GEO Accession
attributes.value: GSM4407796