title: lung microbiome from sputum for E12_47_2.1_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ACGACTACAG for subject 47 at a Exacerbation visit on 04_09_08