title: GSM2307501: ATAC_13; Mus musculus; OTHER
library_construction_protocol: Flash frozen tissue was pulverized in liquid nitrogen using the BioPulverizer (Biospec). This mechanical process breaks open the cells and allows even exposure of intact chromatin to transposase. Pulverized material was thawed in glycerol containing nuclear isolation buffer to stabilize nuclear structure (Zhang et al.; Masuda et al., 1991), and then filtered through Miracloth (Calbiochem) to remove large tissue debris. Nuclei were washed and directly used for treatment with Tn5 transposase. TruSeq libraries were constructed as per manufacturer's recommendations.
attributes.tag: GEO Accession
attributes.value: GSM2307501