title: GSM2983523: HITS-CLIP AEG1 1; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were lysed, partially RNase I-digested, and immunoprecipitated with anti-HA antibody (https://www.thermofisher.com/antibody/product/HA-Tag-Antibody-clone-2-2-2-14-Monoclonal/26183). The RNA moiety was dephosphorylated and radioisotope-labeled. The complexes were subsequently eluted, resolved by SDS-PAGE, transferred to nitrocellulose membranes, and analyzed by phosphorimaging. RNA fragments were extracted by proteinase K digestion from the nitrocellulose membranes and purified by phenol/chloroform extraction. cDNA libraries were constructed by  NEBNext® Small RNA Library Prep Set for Illumina. Libraries were then sequenced on the Illumina HiSeq with 50-nucleotide single-read runs. cDNA libraries were constructed according to the manufacturer's instructions (New England Biolabs; E7330).
attributes.tag: GEO Accession
attributes.value: GSM2983523