title: GSM3903926: Donor HU1020 (AKA BD405A) HBV/HDV co-infected, 28 days, sample 1; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA extracted using Qiagen Rneasy Mini Kit per manufacturer's directions. The integrity of total RNA samples were assessed on Bioanalyzer 2100 using RNA 6000 Nano chip (Agilent Technologies, CA); only samples with RNA Integrity Number (RIN) greater than 9.0 were used for RNA-seq.  The poly-A containing RNA transcripts were enriched from 1μg of total RNA for each sample using oligo-dT bead, and further converted to cDNA then Illumina sequencing library using PrepX RNA-seq library kit on the automated Apollo 324TM NGS Library Prep System (Wafergen Biosystems, CA) according to the manufacturer's protocol.  Different DNA barcodes were added to each RNA-seq library.  The libraries were examined on Agilent Bioanalyzer DNA High Sensitivity chips for size distribution, and quantified by Qubit fluorometer (Invitrogen, CA).  The RNA-seq libraries were pooled at equal molar amount and sequenced on Illumina HiSeq 2500 Rapid flowcells as single-end 75 nucleotide reads following the standard protocol.  Raw sequencing reads were filtered by Illumina HiSeq Control Software, and only the Pass-Filter (PF) reads were used for further analysis.
attributes.tag: GEO Accession
attributes.value: GSM3903926