title: 16S rDNA-Seq: CBS incubation soil
design: The total community DNA was extracted from soils by use of a MoBio Power Soil DNA extraction kit. The 16S rRNA gene was amplified in triplicate using the barcoded 515F and 907R primer set. The ITS gene was amplified in triplicate using the barcoded ITS1F and 2043R primer set.PCR products were quantified, purified and sequenced by Illumina Miseq platform for assessment of bacterial diversity and community structure.