title: GSM4409407: WT_NaCl_rep_4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from sorted LSK cells using the RNeasy Micro kit (Qiagen). RNA quality was checked using the Bioanalyzer Pico kit. RNA with a RIN value of <8 was excluded from library preparation. Double-stranded cDNA was synthesized and amplified using the SMARTer Ultra Low RNA kit (Clontech). The cDNA samples were sheared by the Covaris system. Sheared fragments were end-repaired, A-tailed, and ligated to sequencing adaptors. A size selection to about 200 bp insert size was performed before PCR enrichment.
attributes.tag: GEO Accession
attributes.value: GSM4409407