title: GSM4481476: 12wk_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified with PicoPure Kit (ARCTURUS) from LMD derived endothelial and perivascular cells of the OF. For poly-A dependent cDNA synthesis and a first amplification step the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) was used. After quality control (High sensitivity DNA Kit, Agilent) and concentration measurement (Qubit dsDNA HS Assay Kit, Invitrogen), up to 10 ng  of the purified cDNA was used for tagmentation and library completion with the Nextera XT DNA Sample Preparation Kit  (Illumina). For sequencing, Illumina NextSeq500 device was used generating 75 bp paired-end reads.
attributes.tag: GEO Accession
attributes.value: GSM4481476