title: GSM2460250: chromatin transcribed with complete factors; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: in vitro RNA transcripts were purified by QUAGEN Rneasy plus micro kit, RNA samples were subsequently subjected to 5' RACE. After nested PCR of 5’-RACE samples, DNA was prepared for next- generation sequencing using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) except the end prep step was omitted and in the final purification step 17 μL of 0.1X TE buffer was used to elute the DNA from the AMPure XP beads and 15 μL was reclaimed as the purified RACE library. DNA concentration was determined using a Qubit dsDNA HS Assay Kit (Life Technologies) and DNA integrity determined by running an aliquot on a Bioanalyzer High Sensitivity DNA Chip (Agilent). The library was accurately quantified using a KAPA Library Quantification Kit (KAPA)
attributes.tag: GEO Accession
attributes.value: GSM2460250