title: PacBio sequence of Chromobacterium violaceum ATCC 53434
design: 5ug of genomic DNA were sheared using g-tube (Covaris) to 20kb fragments. The Pacbio library was prepared using standard Pacbio protocol for 20 kb libraries  20kb Template Preparation Using BluePippin Size-selection system . After Blue Pippin (Sage Science) size selection from 10 kb average DNA fragments size of the library was 25kb. Sample library was sequenced on Pacbio RS II instrument with one SMRT cell using P6-C4 chemistry with 6-hour movie.