title: lung microbiome from sputum for E22_78_2.1_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ACTACTATGT for subject 78 at a Exacerbation visit on 05_14_08