title: Illumina MiniSeq paired end sequencing; NGS in hematopoietic cell transplant recipients
design: NGS in hematopoietic cell transplant recipients
library_construction_protocol: Pre-transplant peripheral blood samples  Isolation of genomic DNA  Next generation sequencing (NGS) with a customized complement-related gene panel (complement factor H/CFH, CFH-related, CFI, CFB, CFD, C3, CD55, C5, CD46, thombomodulin/THBD), including TMA-associated ADAMTS13 (A Disintegrin and Metalloproteinase with Thrombospondin motifs). Probes were designed using DesignStudio (Illumina, San Diego, California) to cover all exons spanning 15 bases into the intronic regions (98% coverage). We used 10ng of initial DNA material. The performer is Illumina
attributes.tag: Experimental Factor: disease
attributes.value: TMA