title: 18S amplicon sequencing of SIO pier seawater collected on 7/29/14 and incubated 5 days
design: 600 ml seawater incubated five days (this is the control); 250 ml filtered on 0.2 micron Supor filter; total DNA extracted using phenol-chloroform extraction and Qiagen DNEasy Blood and Tissue kit; sent to Research and Testing Laboratory (Lubbock, TX) for sequencing of 18S amplicon using TAReuk primers (Stoeck 2010)