title: GSM2306333: smallRNA-seq_E14_mESC; Mus musculus; ncRNA-Seq
library_construction_protocol: RNA was isolated using TriReagent (Sigma) and treated with DNaseI (Ambion DNA-free DNA Cat #1311027) using manufacturers instructions Small RNA Sequencing libraries were generated using the Illumina TruSeq Small RNA Library Preparation Kit (RS-200-0012) with the following modifications. 100ng – 1µg total RNA input material was used. cDNA samples were run on 10% Novex PAGE gels for purification and the gel piece between the 145bp and 160bp marker was excised and cDNA was eluted from the gel in freshly prepared 0.3M NaCl by rotation overnight at 4˚C. The cDNA was precipitated in EtOH overnight from the supernatant the cDNA was resuspended in 10µl EB buffer and the library was quantified using the High-Sensitivity DNA chips on the Agilent Bioanalyzer. High-throughput sequencing of all libraries was carried out with a single-end protocol on a HiSeq 2000 instrument (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2306333