title: GSM4158908: LR_CONT_3; Rattus norvegicus; RNA-Seq
library_construction_protocol: One hour after undergoing testing on the elevated plus maze, the animals were sacrificed by rapid decapitation and the brains were flash frozen in isopentane cooled on dry ice. The whole hippocampus was dissected from each brain on a dry ice and wet ice mixture and immediately put in TRIzol™. RNA was extracted using the Zymo RNA isolation Kit. The cDNA library was constructed using 0.1-3ug of total RNA and the Illumina TruSeq Stranded mRNA Library Preparation kit (Catalog #s RS-122-2101, RS-122-2102) (Illumina, San Diego, CA).   The final cDNA libraries were checked for quality once again by TapeStation (Agilent) as well as qPCR through the use of Kapa's library quantification kit for Illumina Sequencing platforms (catalog # KK4835, Kapa Biosystems,Wilmington MA). The samples were clustered on a cBot automated cluster generation system (Illumina) for clonal amplification. The samples were then hybridized to the slide (“flow cell”) of a HiSeq 2000 (Illumina) and underwent non-stranded short read (50-bp length) single-end sequencing in High Output mode using version 3 reagents.
attributes.tag: GEO Accession
attributes.value: GSM4158908