title: Illumina HiSeq 4000 sequencing; RNA-seq of Mus musculus beta cells to determine the role of the miR-200–Zeb1 axis in beta cell tumor development
design: RNA-seq of Mus musculus beta cells to determine the role of the miR-200–Zeb1 axis in beta cell tumor development
library_construction_protocol: islets were isolated from 6-week-old mice and picked manually before being frozen down as a pellet  RNA was extracted using PicoPure columns with on-column DNase treatment. Quality was verified on Bioanalyzer TruSeq Stranded mRNA Library Prep Kit
attributes.tag: Experimental Factor: phenotype
attributes.value: targeted mutation of Zeb1 3'UTR to mutate 2 bp in miR-200c sites and 3 bp in miR-141 sites