title: GSM4481513: KLF5_R1-AD1_DHT_ChIP-seq rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Nuclear extracts were subjected to sonication on dry ice for eight cycles at 40% amplitude using a 450 Sonifer (Branson). Each cycle consisted of 10 s pulse / 10 s rests for 1 min, with 5 min rests between cycles to achieve DNA fragmentation of 200-500 bp. Lysates were pre-cleared by 1h incubation with Protein A/G Plus agarose beads (GE) pre-blocked with tRNA (Sigma). Lysates were immunoprecipitated overnight with 5 ug KLF5 antibody and tRNA-blocked Protein A/G Plus agarose beads. DNA was purified using a PCR purification kit (Qiagen). Rubicon ChIP Sample Preparation Kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4481513