title: degradome sequencing of maize: leaf
design: Total RNA from maize leaves were isolated and purified using poly-T oligo-attached magnetic beads. The 3 cleavage product of the mRNA were ligated with 5 adapters, transcribed to cDNA and followed by PCR amplification. Finally, the 50 bp single-end sequencing on an Illumina Hiseq 2500 were performed.