title: GSM3369579: SKBR3_shCTR_rep1_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from fresh or frozen cell pellets using TRIzol reagent (Thermo Fisher Scientific cat# 15596018) according to manufacturer's instructions. Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit (New England Biolabs cat# E6310) starting with 1µg total RNA and following the manufacturer's instructions. Ribo-depleted RNA was then further processed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs cat# E7420) for library preparation following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3369579