title: GSM2728808: FoxAa>Bra Rep2; Ciona intestinalis; RNA-Seq
library_construction_protocol: 400 embryos were collected at Hotta stage 19.5 into RNA lysis buffer and stored at -80C until RNA purification.RNA was purified with the Zymogen Quick-RNA miniprep kit (R1054) and eluted in 50 ul elution buffer. Final purified RNA concentration was 52-60 ng/ul. Libraries were constructed with the TruSeq stranded mRNA library kit (Illumina) using standard protocols. Libraries were quality checked with an Agilent Tapestation and quantified by qPCR.
attributes.tag: GEO Accession
attributes.value: GSM2728808