title: GSM2641063: ATAC_BiMaleDM21; Mus musculus; ATAC-seq
library_construction_protocol: Embryonic gonads were micro-dissected at according stage, removed from the mesonephros and incubated in 0.05% trypsin for 5 minutes at 37C. Gonads were then mechanically dissasociated in PBS/3% BSA and passed through a filter cap to obtain a single cell suspension. Fluorescently-labeled cells were collected by Fluorescence Activated Cell Sorting (FACS). For ATAC-seq, 60K cells were collected per replicate. For ChIP-seq, 100K cells were collected per replicate. ATAC-seq libraries were prepared according to Buenrostro et al.2013. ChIP-seq libraries were prepared according Van Galen et al., 2016.
attributes.tag: GEO Accession
attributes.value: GSM2641063