title: GSM2548611: Endothelin-1 + Vehicle_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using QIAzol Lysis Reagent (Qiagen). Whole transcriptome barcoded cDNA library is first generated with SuperScript® VILO™ cDNA Synthesis kit (Thermo Fisher Scientific) from 20 ng of total RNA. Then cDNA is amplified using Ion AmpliSeq™ technology to accurately maintain expression levels of all targeted genes. Amplified cDNA Libraries were evaluated for quality and quantified using Ion Library TaqMan Quantitation Kit (Thermo Fisher Scientific). Libraries were then diluted to 75 pM and pooled equally, with fourteen individual samples per pool.
attributes.tag: GEO Accession
attributes.value: GSM2548611