title: GSM2111795: Swertia_japonica_roots; Swertia japonica; RNA-Seq
library_construction_protocol: The frozen samples of S. japonica were powdered using a multi beads shaker (Yasui Kikai, Japan), and were used for the subsequent RNA extraction. Total RNA for each tissue types were isolated using RNeasy Plant Mini Kit (Qiagen, USA) according to the manufacturer’s instruction. The extracted RNA samples were treated with RNase free DNase (Takara, Japan), purified by column binding, and further purified through ethanol precipitation. RNA quality for each sample was assessed by the Agilent Bioanalyzer 2100 (Agilent Technology, USA), and samples with RNA integrity number (RIN) value above 8 were selected for the cDNA library preparation. For the cDNA library construction, firstly, mRNA for each samples were isolated from the total RNA using beads with oligo (dT). mRNA thus obtained were added with the fragmentation buffer to shear it in the small fragments, which were used as templates for the synthesis of the first strand of cDNA using random hexamer primers. cDNA library was then prepared using SureSelect Strand Specific RNA library kit (Agilent Technology, USA) according to the manufacturer’s instruction.
attributes.tag: GEO Accession
attributes.value: GSM2111795