title: PacBio sequencing of H9hESC Enginereed LINE-1 retrotransposition assay : Biological Replicate 10
design: gDNA collected from Engineered LINE-1 retrotransposition assay was randomly sheared to 3kb fragments and adapters ligated onto end-repaired, dA-tailed products.  A round of amplification involved a biotinylated primer specific to the Engineered LEAP sequence and biotinylated products were captured and subjected to a round of PCR involving an SV40pA primer and Adapter primer.  Resulting amplicons were sequenced on the PacBio RS II sequencing machine