title: GSM4658243: SAM24362767; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspensions were processed using Chromium™Single Cell 3' Reagent Kits v2 (10X Genomics PN-120237 and PN-120236) following manufacturer's user guide. Cell-RT mix was prepared to aim 10,000 cells per sample and applied to Chromium™ Controller for GEM generation and barcoding. Then samples were subjected to post GEM-RT cleanup, cDNA amplification (10 cycles), and library construction according to the user guide. Sample index PCR was done with 12 cycles. Libraries were then quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Q33230) and profiled by Bioanalyzer High Sensitivity DNA kit (Agilent Technologies 5067-4626). Libraries were sequenced by HiSeq4000 (Illumina) following 10X Genomics sequencing specification cDNAs and libraries were prepared according to manufacturer instructions (10× Genomics)
attributes.tag: GEO Accession
attributes.value: GSM4658243