title: GSM2641094: 12h; Locusta migratoria; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from four abdomens samples by using of TRIzol reagent (Invitrogen, USA) following the manufacturer’s instructions. small RNA was the 18-30 nt stripe and recovered from the different size RNA segments which separated by the PAGE gel. Then the 3’ and 5’ ligation, reverse transcription and polymerase chain reaction amplification were conducted. PCR products were purified by PAGE gel and cDNA libraries of small RNAs were sequenced using an Illumina/Solexa high-throughput sequencing technology
attributes.tag: GEO Accession
attributes.value: GSM2641094