title: GSM4600976: BC5 3; Homo sapiens; RNA-Seq
library_construction_protocol: The tissue pieces were digested with 2 mL of GEXSCOPETM tissue dissociation solution (Singleron Bio Com, Nanjing, China) at 37 °C for 15 min with sustained agitation. After digestion, the samples were filtered through 40-µm sterile strainers and centrifuged at 1,000 rpm for 5 min. Then the supernatants were discarded, and the cell pellets were suspended in 1 mL phosphate-buffered saline (PBS; HyClone). To remove red blood cells, 2 mL of GEXSCOPETM red blood cell lysis buffer (Singleron) was added, and cells were incubated at 25 °C for 10 min. The solution was then centrifuged at 500 g for 5 min and resuspended in PBS.  Single cells were encapsulated into emulsion droplets using the Chromium Controller (10 x Genomics). The scRNA-seq libraries were constructed using the Chromium Single Cell 3' Library, Gel Bead & Multiplex Kit (10x Genomics)
attributes.tag: GEO Accession
attributes.value: GSM4600976