title: GSM3820339: WT ZIKV Rep3; Aedes aegypti; RNA-Seq
library_construction_protocol: Mosquitos (10 per sample) were homogenised in 1ml TRIreagent for 5min at 30Hz using Tissue Lyser II (Qiagen, USA), then total RNA was isolated according to the manufacturers instructions. RNA samples from pooled 10 Aedes aegypti mosquitos infected with WT or xrRNA2' mutant Zika MR766 or mock-infected were subjected to ribosomal RNA depletion using Ribo-Zero Gold rRNA Removal Kit (Illumina, USA) followed by library preparation using TruSeq 2 Library Preparation Kit (Illumine, USA). Three biological replicates (pools of mosquitos) were used in the experiment.
attributes.tag: GEO Accession
attributes.value: GSM3820339