title: GSM3138077: day4_eb; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell samples were prepared by dissociating incubating them in TrypLE for 2 minutes at 37C and then vortexing them briefly. Cells were then strained them with a 30 µm cell strainer (Miltenyi Biotec). The strained cells were washed twice with dPBS containing 0.04% bovine serum albumin (BSA, Sigma) and then counted.  The concentration of the cells was adjusted to 450-500 cells/uL and then 10,000 cells from each sample underwent library preparation. Single-cell RNA-Seq libraries were prepared using GemCode Single-Cell 30 Gel Bead and Library Kit (now sold as P/N 120230, 120231, 120232, 10x Genomics) as indicated in Zheng et al 2017) . [citations] Zheng, G.X.Y., Terry, J.M., Belgrader, P., Ryvkin, P., Bent, Z.W., Wilson, R., Ziraldo, S.B., Wheeler, T.D., McDermott, G.P., Zhu, J., Gregory, M.T., Shuga, J., Montesclaros, L., Underwood, J.G., Masquelier, D.A., Nishimura, S.Y., Schnall-Levin, M., Wyatt, P.W., Hindson, C.M., Bharadwaj, R., Wong, A., Ness, K.D., Beppu, L.W., Deeg, H.J., McFarland, C., Loeb, K.R., Valente, W.J., Ericson, N.G., Stevens, E.A., Radich, J.P., Mikkelsen, T.S., Hindson, B.J., Bielas, J.H., 2017. Massively parallel digital transcriptional profiling of single cells. Nat. Commun. 8.
attributes.tag: GEO Accession
attributes.value: GSM3138077