title: GSM2306997: 18-G3.CC019; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were isolated from two normal controls and six IPF tissue samples prepared as described for FACS except that cells were sorted initially with CD326 prior to single cell isolation.  For single cell analysis cell capture, lysis, reverse transcription and cDNA amplification were performed on the C1 IFC for mRNA-seq on Fluidigm C1 Single-Cell Auto Prep System following the manufacturer’s protocol. Medium sized C1 mRNA-Seq chips were used to capture each cell-cycle fraction.  The C1 Auto-prep system captures the dissociated single cells across 96 wells and performs cell lysis, cDNA synthesis with reverse transcription and PCR reaction using the SMARTer Ultra Low Input RNA Kit v3 (ClonTech, Mountain View, CA).  Cells captured across the 96 wells are manually inspected as a quality control measure to remove empty well, doublets or debris containing wells.  cDNA from several representative cells are checked by High Sensitivity DNA chips using Fragment Analyzer (Advanced Analytical) and all cDNA libraries are quantitated via Qubit (Thermo Fisher Scientific, Carlsbad, CA).  Libraries for each of the 96 captured cells are prepared using the Illumina Nextera XT DNA sample preparation kit with 96 dual barcoded indices
attributes.tag: GEO Accession
attributes.value: GSM2306997