title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of wild-type, Mbd3 and Sall4/1 mutant mouse embryonic stem cells
design: RNA-seq of wild-type, Mbd3 and Sall4/1 mutant mouse embryonic stem cells
library_construction_protocol: ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. For neural differentiation, ES cells were plated onto laminin-coated plates in N2B27 media at a density of 100,000 per 6-well. Medium was replaced every day and the cells were not passaged during the timecourse. RNA was extracted with TRI Reagent (Sigma). Libraries were prepared with the NEXTflex Rapid Directional Illumina RNA-seq Library Prep Kit (Bioo Scientific)
attributes.tag: Experimental Factor: Sall4flox/flox
attributes.value: genotype