title: GSM2108662: Bulk leukemic cells from NPM1c+ AML humanized mouse 1; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow cells were extracted from humanized NSG mice with AML. Cells were labeled with anti-CD123 and anti-CD33 antibodies followed by cell sorting with an ARIA cell sorter. Populations expressing GFP and CD33 (AML bulk leukemic cells) and populations expressing GFP, CD33 and CD123 (AML LSCs) were sorted. RNA was extracted with the RNeasy micro kit from Qiagen. cDNA was generated and amplified with the NUGEN OVATION kit. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2108662