title: GSM4600725: RL lenalidomide -  input_DNA_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP samples were prepared using the SimpleChIP Enzymatic Chromatin IP Kit, based on the manufacturer's instructions (Cell Signaling, #9003). Briefly, 4 million RL cells were incubated for 48 hours with either DMSO, 10μM lenalidomide, 5μM tazemetostat or combination, followed by cross-linking using 1% formaldehyde. Cells were lysed and chromatin was digested for 20 min using 0.5μl micrococcal nuclease. Nuclei were sonicated After purification, DNA was used as an input for next-generation sequencing library construction using the SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina (Cell Signaling, #56795) following manufacturer's protocol and sequenced on a NextSeq SR25 platform (Illumina) after standard library QC.
attributes.tag: GEO Accession
attributes.value: GSM4600725