title: GSM2982986: Vehicle control; Mus musculus; MBD-Seq
library_construction_protocol: Genomic DNA was extracted using phenol/chloroform method. Extracted DNA was sonicated with Picoruptor (Diagenode, Seraing, Belgium) to obtain ~300 bp fragments. MBD2-mediated enrichment of methylated DNA was performed using the methylated DNA enrichment kit, EpiXplore (TAKARA, Japan) according to the manufacturer's instructions. MBD-Seq libraries were prepared using QIAseq Ultralow Input Library Kit (Qiagen) according to the manufacturer's instructions and validated for an average size of about 300-700 bp using a TapeStation and the Agilent High Sensitivity D1000 ScreenTape kit.
attributes.tag: GEO Accession
attributes.value: GSM2982986