title: GSM3592122: MeV-IC323-mCherry-uN genomes passaged 1x on H358 cells (E1-2nd); Measles morbillivirus; RNA-Seq
library_construction_protocol: Viral ribonucleocapsids were purified from cellular RNA through CsCl density gradient centrifugation. Viral genomic RNA was extracted from ribonucleocapsids with phenol:chloroform:isoamyl alcohol (25:24:1) and then ethanol-precipitated. 0.5 μg viral genomic RNA was fragmented for 7 min using the Ambion Fragmentation Reagent according to the manual. Fragmented RNA was purified by phenol:chloroform:isoamyl alcohol phase separation and precipitated using ethanol/sodium-acetate. RNA pellets were washed with 70% (w/v) ethanol, dried and resuspended in 11 μl nuclease-free water. The concentration and integrity of the RNA was assessed on an Agilent Bioanalyzer DNA 100 chip (Agilent, Santa Clara, CA). cDNA libraries were generated using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Illumina, San Diego, CA) according to the manufacturer's protocol. 300 x 2 paired end sequencing of each library was performed on an Illumina MiSeq using MiSeq v2 sequencing kit and MCS v2.6.2.1 collection software. Base-calling was performed using Illumina's RTA version 1.18.54.
attributes.tag: GEO Accession
attributes.value: GSM3592122