title: RNA-seq of Melanochromis auratus brain (dark morph)
design: RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturers protocol. Briefly, 1g RNA was put into mRNA selection by poly-T oligo attached magnetic beads followed by fragmentation (94C for 6 min). The cleaved mRNA fragments were reverse transcribed into first-strand cDNA using GoScript Reverse Transcriptase (Promega) and random hexamer primers (Illumina). We used Illumina-supplied consumables to synthesize second-strand cDNA following by adenylating 3 ends. Barcoded adapters from TruSeq RNA CD Index Plate (Illumina) were ligated to the ends of the double-strand cDNA. The final libraries were amplified using 15 PCR cycles and quantified and quality-assessed using an Agilent DNA 12000 Kit on a 2100 Bioanalyzer (Agilent). Indexed DNA libraries were normalized. Libraries were sequenced on a HiSeq X Ten platform (BGI Genomics, Beijing).