title: GSM3138372: day0_mESC biol rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Embryoid bodies were dissociated by incubating them in TrypLE for 2 minutes at 37C and then vortexing them briefly. Total RNA was extracted from the counted cells using the Sigma GenElute Mammalian Total RNA kit. Libraries were prepared according the TruSeq Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E). [citations] Craft, A.M., Ahmed, N., Rockel, J.S., Baht, G.S., Alman, B. a, Kandel, R. a, Grigoriadis, A.E., Keller, G.M., 2013. Specification of chondrocytes and cartilage tissues from embryonic stem cells. Development 140, 2597–610. https://doi.org/10.1242/dev.087890
attributes.tag: GEO Accession
attributes.value: GSM3138372