title: GSM4158563: Kidney1; Mus musculus; RNA-Seq
library_construction_protocol: Kidneys were harvested and stored in DMEM on ice. Kidneys were dissociated with 0.2 mg/mLof Liberase TM in DMEM at37◦C 800 rpm for 45 minutes (Thermomixer C). Aliquots were taken and visualized under the microscope until tissue was completely dissociated. Liberase TM was inactivated with DMEM+10% fetal bovine serum (FBS) and dissociated cells were filtered through a100μm and 40μm filter. Dissociated cells were then spun down at 200×g for 5 minutes at 4◦C and resuspended in PBS+1% BSA. We loaded a total of 80,000 sorted cells into two wells on the 10x Chromium system (40,000 per well; 10X Genomics). We generated barcoded cDNA libraries using the Chromium Single Cell 3' Library Construction Kit (V3; 10XGenomics).
attributes.tag: GEO Accession
attributes.value: GSM4158563