title: GSM1914920: day0 RT1 feces; mouse gut metagenome; OTHER
library_construction_protocol: Bacterial DNA content was extracted using QIAamp Fast DNA stool Mini Kit (Qiagen). 40 DNA isolates from mice feces and 9 biospecimens of mice cecal content were received in Second Genome’s service laboratory on March 28 and April 2, 2014. The samples arrived intact, frozen and according to specification, and were immediately stored at -20°C. The biospecimen’s DNA are isolated using the MoBio PowerMag DNA isolation kit as per vendor’s protocol. The combined DNA samples ranged in concentration of 25-159.8 ng/μl. All samples were quantified via the Qubit® Quant-iT dsDNA Broad-Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA. The sample set met specifications. To enrich the sample for bacterial 16S V4 rDNA region, DNA was amplified utilizing fusion primers designed against the surrounding conserved regions which are tailed with sequences to incorporate Illumina (San Diego, CA) flow cell adapters and indexing barcodes. Each sample was PCR amplified with two differently bar coded V4 fusion primers. 49 samples met the post-PCR quantification minimum and were advanced for pooling and sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1914920