title: GSM2805948: 0_1b_S2_R1_001.fastq.gz; Mus musculus; RNA-Seq
library_construction_protocol: Mouse hippocampus and PFC were Microdissections under stereomicroscope, then flash frozen on dry ice and stored at -80°C until processed for nuclei isolation. Tissue samples were cut into pieces < 0.5 cm and homogenized using a glass dounce tissue grinder in 2ml of EZ PREP buffer, and incubated on ice for 5 minutes, with additional 2 ml ice-cold EZ PREP. Nuclei were centrifuged at 500 x g for 5 minutes at 4°C, washed with 4 ml ice-cold EZ PREP and incubated on ice for 5 minutes. After centrifugation, the nuclei were washed in 4 ml buffer (1x PBS, 0.01% BSA and 0.1% RNAse inhibitor). DroNc-seq libraraies were constructed following the step by step protocol: http://dx.doi.org/10.1038/protex.2017.094
attributes.tag: GEO Accession
attributes.value: GSM2805948