title: GSM4481289: A8 Mock; Homo sapiens; RNA-Seq
library_construction_protocol: Single cells were loaded to 10x Genomics Chromium chip (10x Genomics, Pleasanton, CA, USA) to generate single-cell Gel bead in Emulsion (GEM). GEM-reverse transcription was performed with the following condition: 55 ℃ for 2 h, 85 ℃ for 5min; held at 4 ℃. Single-cell RNA-Seq libraries were prepared using GemCode Single-Cell' Gel Bead and Library Kit. After RT, GEMs were broken, and the single-strand cDNA was cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific) and SPRIselect Reagent Kit (0.6x SPRI; Beckman Coulter). cDNA was amplified using with the following condition: 98 ℃ for 3min; cycled 14x: 98 ℃ for 15 s, 67 ℃ for 20 s, and 72 ℃ for 1min; 72 ℃ for 1min; held at 4 ℃. cDNA product was cleaned up using the SPRIselect Reagent Kit. Indexed sequencing libraries were constructed with the reagents in the GemCode Single-Cell 3' Library Kit.
attributes.tag: GEO Accession
attributes.value: GSM4481289