title: D5 normalized cDNA using Titanium Tech.
design: RNA was extracted from various plant organs (stem, embryo   fiber, leaf, whole seedling, buds) using a modified hot-borate method.  cDNA was synthesized with a poly-T primer and amplified using the Clontech SMRT technology.  The amplified library was normalized using the Evrogen Trimmer kit (DSN).  The normalized library was PCR titered, and re-amplified prior to ligation of 454 sequencing adaptors.  The 5' and internal coding regions were preferentially amplified using ligation mediated PCR-suppression.  The Titanium bead library was sequenced using the 454 Titanium chemistry.  The raw 454 reads contain adaptor sequences. These D5 Titanium reads are in F29QY3E01.sff