title: GSM4106239: S3_S11; Malus domestica; Bisulfite-Seq
library_construction_protocol: DNA was extracted using NucleoSpin Plant II kit (Macherey-Nagel, Hoerdt, France). The manufacturer's recommendations were applied with the next modifications: at step 2a PL1 buffer quantity was raised to 800µL and PVP40 was added at 3% of final volume, suspension was then incubated 30min at 65°C under agitation. The lysate solution was centrifuged 2min at 11000g before transferring the supernatant in step 3. At step 4 PC buffer was raised to 900µL. In step 6 the first wash was decreased to 600µL and the third wash was raised to 300µL. An extra-centrifuge step was added after washing to remove ethanol waste from the column. In step 7 DNA was eluted twice in 55µL in total. Made by sequencing platerform
attributes.tag: GEO Accession
attributes.value: GSM4106239