title: Endophytes of rice seeds
design: Rice seeds DNA was extracted with FastDNA Spin kit for Soil DNA extraction kit. The V4 region of the 16S rRNA gene was amplified in triplicate using the barcoded 534F and 783R primer set. PCR products were quantified, purified and sequenced by Illumina Hiseq PE250 platform for assessment of bacterial endophytes diversity and community structure.