title: 16S rDNA library from Nahant Canoe Cove: 0.2 um fraction
design: V4 variable region of the 16S rDNA from the 0.2 um fraction of a serially filtered water (63um, 5um, 1um, and 0.2 um). V4 amplified with U515 and E786R with a two step PCR method, and identified in a multiplexed sampled by a sample spcecific barcode