title: lung microbiome from sputum for 35B_180_3.1_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ACGACTACAG for subject 180 at a Exacerbation visit on 04_13_10