title: Illumina MiSeq paired end sequencing; Targeted sequencing of 86 regulatory elements and 12 breast cancer genes
design: Targeted sequencing of 86 regulatory elements and 12 breast cancer genes
library_construction_protocol: 500 ng of DNA was used. Extension-ligation was performed according to manufacturer’s protocol. Two PCR cycles were performed as follows: 95°C 3 minutes, followed by 2 cycles of 95°C for 30 seconds, 66°C for 30 seconds, and 72°C for 60 seconds. Following two cycles of PCR, Ampure Bead cleanup was carried out according to standard protocol and eluted in 25 uL.  20 uL of PCR product, 22 uL of PMM2/TDP1 and 4 uL of the i7 primer supplied with from the TruSeq Custom Amplicon Index Kit were added to PCR tubes, and PCR was carried out using the following conditions: 95°C for 30 seconds; 22 cycles of 95°C for 30 seconds, 66°C for 30 seconds, and 72°C for 60 seconds; and finally 72°C for 5 minutes
attributes.tag: Experimental Factor: individual
attributes.value: AA1842
attributes.tag: Experimental Factor: disease
attributes.value: breast carcinoma