title: 18S rDNA library from Nahant Canoe Cove: 63 um fraction
design: V9 variable region of the 18S rDNA from water collected through a plankton net (63um) in a Oakridge tube and concentrated on a 0.2 um filter. V9 amplified with 1391F and EukB with a two step PCR method, and identified in a multiplexed sampled by a sample spcecific barcode