title: GSM4260060: iLSC-control-rep3 [ATAC-seq]; Homo sapiens; ATAC-seq
library_construction_protocol: nuclei were isolated by washing with ATAC buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2) and lysing with 50 ul of ATAC lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40). 1ml of ATAC buffer was added to dilute lysis buffer Cell lysates were spun to obtain nuclear pellets, which were subjected to transposase reaction using the Illumina Nextera DNA Sample Preparation Kit according to the manufacturer's instructions. The reaction was stopped by adding SDS to a final concentration of 0.2%. 
attributes.tag: GEO Accession
attributes.value: GSM4260060