title: GSM3730494: Input 4082 1; Caenorhabditis elegans; ChIP-Seq
library_construction_protocol: Lysates were sonicated and cleared by centrifugation. Protein-DNA complexes were pulled-down using GFP-TRAP (Chromotek). Libraries were prepared by using the Illumina TruSeq DNA Nano Kit with some modifications. The preparation was started with the end repair step of the protocol. Up to 100ng ChIP/Input DNA was used as starting material. After end repair and A-tailing dual indexing adapters were ligated. The products were then purified and amplified (12 PCR cycles for the Input Samples and 15 cycles for the ChIP Samples) to create the final libraries. After validation (Agilent 4200 TapeStation) each single library was quantified by using the Roche KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System. The libraries were then pooled equimolar. Finally the pool was sequenced on one Illumina HiSeq4000 lane 1x51bp (51+8+8).
attributes.tag: GEO Accession
attributes.value: GSM3730494