title: GSM3671372: WT_0hr_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using TRIzol (Invitrogen) and RNeasy Mini Kit (QIAGEN catalog # 74104) according to instructions provided by the manufacturer. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 2 ng total RNA with RNA integrity numbers ranging from 8.1 to 9.6 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech catalog # 63488), with 12 cycles of amplification. Subsequently, 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit (Illumina).  An average of 56.8 million paired reads were generated per sample and the percent of mRNA bases per sample ranged from 58% to 75%.
attributes.tag: GEO Accession
attributes.value: GSM3671372