title: GSM4106320: C102_plus_2 Sample 37; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested by trypsinisation (0.5% trypsin-EDTA, Invitrogen) and washed once with phosphate-buffered saline and twice with 0.9% NaCl, followed by centrifugation at  4C (16100 × g for 5 min) to obtain cell pellets. Total RNA was isolated from the cell pellets from the T75 cultures using TRIzolTM Reagent (ThermoFisher Scientific) using supplier protocol for RNA extraction. RNA pellets were dissolved in 50uL of RNase free water, and RNA concentrations were measured using NanoDropTM 2000 Spectrophotometer (ThermoFisher Scientific).  Sequence libraries were generated using the Nextflex Rapid Illumina Directional RNA-Seq Library Prep Kit (Bioo Scientific) using the Sciclone NGS Liquid Handler (Perkin Elmer).
attributes.tag: GEO Accession
attributes.value: GSM4106320