title: GSM3822156: 10 ng/ml HHQ replicate 2, 24 hours after treatment; Emiliania huxleyi CCMP1516; RNA-Seq
library_construction_protocol: Cell biomass was collected via centrifugation of 400 ml of culture and total RNA extracted using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer's recommendations using 350 µl RLT plus buffer per sample and the optional centrifugation (14,000 RPM for 1 min) step to ensure membranes were dry prior to elution with 30 µl RNase free water.  Eluent was reapplied to the membrane, and incubated for 8 min at room temperature before eluting again to increase yield. Strand-specific RNAseq library construction performed using the KAPA Stranded mRNA-Seq library preparation kit with KAPA mRNA capture beads (Kapa Biosystems).