title: GSM3592229: HFD_mir181DKO2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from epididymal white adipose tissue (eWAT) from WT and miR-181-double knockout mice following 12 weeks on high fat diet using a combination of TRIzol (Invitrogen) and RNeasy Mini columns (QIAGEN). TRIzol-chloroform extractions were precipitated with ethanol, applied to RNeasy Mini columns, and processed according to the manufacturer's instructions. RNA libraries were generated from 1 µg of high-quality RNA from each sample using the TruSeq Stranded mRNA kit (Illumina). Library quality and concentration were assessed by TapeStation and qPCR respectively. Libraries were 75 base-pair single-end sequenced on the NextSeq550 machine (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3592229