title: GSM4660886: post-mortem tissue [CGND-HRA-00219]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA is extracted from flash frozen post-mortem tissue using Trizol/Chloroform, followed by Qiagen RNeasy minikit column purification. RNA is quantified on a Nanodrop 2000 and Qubit™ 2.0 Fluorometer; the quality and integrity of the RNA are verified on an Agilent Bioanalyzer. Starting from 500 ng of total RNA, rRNA is depleted and cDNA libraries prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (KAPA, Roche) and either i) Illumina platform-compatible PCR primers (NEXTflex RNA-seq indexes, BioScientific) for the manual  library preparation protocol and sequencing on Illumina HiSeq 2500 or ii) Illumina sequencing adapters with dual indexes (IDT for Illumina – TruSeq DNA UD Indexes Cat# 20022370) and amplified with 9 cycles of PCR for sequencing on the NovaSeq 6000 (automated library preparation protocol). Libraries are ~ 500bp in length and sequenced PE 125 (HiSeq 2500) or PE 100 (NovaSeq 6000).
attributes.tag: GEO Accession
attributes.value: GSM4660886