title: Illumina HiSeq 2000 sequencing; Functional and transcriptional heterogeneity of human hemopoietic lympho-myeloid progenitors
design: Functional and transcriptional heterogeneity of human hemopoietic lympho-myeloid progenitors
library_construction_protocol: 100 (or less, if 100 were not available) highly purified Haematopoietic stem and progenitor cells (HSPCs) from cord blood samples were sorted directly into lysis buffer in RNAse inhibitor (Clontech) and stored at -80°C before further processing. cDNA synthesis was done with Smarter Ultra low input RNA kit v1 (Clontech). Illumina libraries were generated using Nextera XT DNA (Illumina). Library size and quality were checked using Agilent High-Sensitivity DNA chip with Agilent Bioanalyser (Agilent Technologies). Concentration of indexed libraries was determined using Qubit High-Sensitivity DNA kit (Invitrogen).
attributes.tag: Experimental Factor: lymphoid-primed multipotent progenitor
attributes.value: cell type