title: GSM4478333: BS_F2(met1-6 X vim123)_rep1; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: WGBS: Genomic DNA was isolated from rosette leaves using the Nucleon Phytopure genomic DNA extraction kit (GE Healthcare). WGBS: Genomic DNA was fragmented using Covaris S220, and the size between 300-450bp were gel-purified. The libraries were prepared using TruSeq DNA LT Sample Prep Kit (Illumina) and subjected to bisulfite conversion using MethylCode Bisulfite Conversion Kit (Life Technologies). Bisulfite-treated DNA were amplified with KAPA HiFi HotStart Uracil ReadyMix (Kapa Biosystems), and then purified with Agencourt AMPure XP (Beckman Coulter).
attributes.tag: GEO Accession
attributes.value: GSM4478333