title: RNA-seq of H10407 infected diarrhea from volunteer #5
design: Trizol extraction of RNA from RNA-later preserved H10407 infected stool samples.  Eukaryotic and bacterial rRNA was removed using Ribozero Kit, DNA removed using TurboDNAse and confirmed DNA negative before library building with NEB kit. For Mapping to ETEC genome, reads were shortened to 21bp and mapped with 100% exact homology.