title: GSM1859707: MEFs_siRrm2; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated using the Blood and Cell Culture Mini Kit (Qiagen) with RNase A treatment (Life Technologies). The RRBS libaries were generated at previously described by Orozco et al. with minor modifications. DNA purifications for each enzymatic reaction was carried out using AMPure XP beads (Beckman Coulter). Bisulfite conversion was performed using the Epitect kit (Qiagen) twice compared to manufacturer’s instruction to optimize the efficiency. Bisulfite-converted libraries were amplified using MyTaq Mix (Bioline) with the following program: (98° for 15 sec, 60° for 30 sec, 72° for 30 sec) 12 cycles, 72° for 5 min, 4°C storage.
attributes.tag: GEO Accession
attributes.value: GSM1859707