title: Gene expression profile to Colossoma macropomum exposed to climate change: Sample 15days_a2scenario
design: "The present study aimed to study the effect to climate change in an Amazonian fish called Colossoma macropomum (tambaqui). We exposed the tambaqui species to climate scenarios provided by Intergovernmental Panel on Climate Change (IPCC) to 2100. Fishes were kept in microcosms with electronic control of temperature, CO2 level, humidity and photoperiod. Four levels of temperature and CO2 were tested: - Control Scenario: using an electronic sensor the temperature and CO2 level were kept in real time equal a preserved forest area. - Soft Scenario (B1): 1.5 degrees Celcius above of scenario control and 600 ppm of CO2. - Intermediate Scenario (A1B): 2.5 degrees Celcius  above of control scenario and 800 ppm of CO2. - Extreme Scenario (A2): 4.5 degrees Celcius above of control scenario and 1250 ppm of CO2. After five and fifteen days six animals were euthanased and a sample of white muscle were collected to build eight RNA-seq libraries."