title: NextSeq 500 paired end sequencing; RNA-seq to investigate gene expression profile associated with long-non coding RNA RAIN  in TPC1 cells
design: RNA-seq to investigate gene expression profile associated with long-non coding RNA RAIN  in TPC1 cells
library_construction_protocol: Cells were treated with LNA gapmer oligonucleotides at 25 nanomolar either against the long non-coding RNA RAIN or negative control antisense oligonucleotides. Cells  collected 48h after reverse transfection. Long non-coding RAIN was described by Sancisi et al. in: RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN. Nucleic Acids Res. 2017 doi: 10.1093/nar/gkx802. Genebank sequence IDs: MH084478 - MH084485 RNA was extracted using Qiagen RNAeasy collection kit Library were prepared using Truseq Stranded mRNA sample preparation kit (Illumina) starting from 1 microgram of total RNA
attributes.tag: Experimental Factor: phenotype
attributes.value: wild type phenotype