title: GSM4483236: B_X8_S5; Homo sapiens; RNA-Seq
library_construction_protocol: Sorted basal cells were centrifuged at 300 x g for 5 mins at 4°C, resuspended in a 2:1 mix of Arcturus PicoPure Extraction buffer/RNAlater (Life Technologies) and stored at -80°C until use. samples were thawed, disrupted by lysis (incubation at 42°C for 30 mins followed by incubation at room temperature for 5 mins), vortexed and filtered using RNAeasy MinElute columns (Qiagen). RNA extraction was performed using the Arcturus PicoPure RNA Isolation Kit (Life Technologies Ltd; KIT0204) as per the manufacturer's instructions. RNA was quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific). Libraries were created by UCL Genomics Core Facility using the SMARTer Stranded Total RNAseq Kit (Clontech), cleaned using JetSeq (Bioline) and quality control analysis of RNA integrity was performed using High Sensitivity RNA ScreenTape and the TapeStation Analysis software (Agilent Technologies). RNA sequencing was performed using 0.5x NextSeq for 75 cycles (Illumina; 43PE, ~33M reads per sample; R2 contains UMI information for associated R1 file).
attributes.tag: GEO Accession
attributes.value: GSM4483236