title: GSM2724493: WT-2; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: Cell pellets harvested from mid-log phase cultures were homogenised using glass beads (0.5 mm; BioSpec, Bartlesville, OK) and Fastprep homogenizer (MP Biomedicals, Switzerland). RNA samples were extracted using TRIzol (Thermo Scientific, Waltham, MA) and purified by phenol:chloroform:isoamyl alcohol (25:24:1) (PCI) (Nacalai Tesque, Kyoto, Japan) extraction thrice. Total RNA (50 μg) was treated with DNase I (Thermo Scientific) in the manufacturer-supplied buffer following the manufacturer’s protocol. RNA-seq and primary data analyses were supplied by BGI (Shenzhen, China) using an Illumina HiSeqTM 2000 platform (Illumina, San Diego, CA).
attributes.tag: GEO Accession
attributes.value: GSM2724493