title: GSM2806518: HFD-Only.19; Mus musculus; RNA-Seq
library_construction_protocol: Mice were sacrificed and whole brains were dissected and snap-frozen in dry-ice cold isopentane. CVH punches were conducted in a cryostat on RNAse-free microscope slides. RNA was extracted using the Qiagen Rneasy Mini Kit, according to manufacturers’ protocols (using RLT buffer). CVH punches from two animals of the same condition wereequally pooled (300 ng total) to reduce technical variability. The TruSeq Stranded mRNA Sample Preparation Kit was used to prepare samples for Illumina sequencing according to the manufacturer’s protocol for polyA enrichement (n=6 per condition).  Following preparation, library quality was assessed using a Qubit, Bioanalyzer, and Kapa; 3 samples did not meet QC standards and were omitted from sequencing. Libraries from samples which passed QC (21 samples)whereequimolarly pooled together and sequenced using an Illumina HiSeq 4000 (100 bp single-end reads).
attributes.tag: GEO Accession
attributes.value: GSM2806518