title: Study of the bacterial composition  contents of a variety of bacteria changed in human upon the stimulation of vibration.
design: All PCR reactions were carried out in 30L reactions with 15L of Phusion High-Fidelity PCR Master Mix (New England Biolabs); 0.2M of forward and reverse primers, and about 10 ng templates DNA. Thermal cycling consisted of initial denaturation at129 fo8<<t 98 for 10 s, annealing at 50 for 30 s, and elongation at 72 for 30 s. Finally 72 for 5 min.