title: DNA seq of Acer macrophyllum: seeds
design: DNA extraction was done from 50 mg of 100 surface sterilized, ground seeds using DNeasy PowerPlant Pro Kit (Qiagen). Amplification of ITS2 region by three separate PCRs was done with 5.8S-Fung and ITS4-Fung primers  (Taylor et al. 2016). PCRs were pooled and sequenced.