title: GSM2549662: oxidized rep1 nextseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction using PureLink® RNA Mini Kit (ThermoFisher Scientific, 12183025) and PureLink® DNase Set (ThermoFisher Scientific, 12185010) following kit protocols. Processed RNAs 3' are first ligated to a preadenylated and 3' blocked DNA linker. RT primer (includes a 6-base randomers) is then annealed with the ligation product. Then a 5' RNA linker is ligated. RT is subsequently performed followed by PCR amplification with Illumina compatible primers (adapter+barcodes included) to generate the final sequencing library.
attributes.tag: GEO Accession
attributes.value: GSM2549662