title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of human peripheral blood B helper T-cell subsets
design: RNA-seq of human peripheral blood B helper T-cell subsets
library_construction_protocol: Peripheral blood mononuclear cells were magnetically enriched for CD4+ T-cells with a kit (Miltenyi). T-cells were stained with antibodies to CD4, Il-7R, CD25, CXCR5, CCR6, CXCR3, CD161 and CCR5 and purified on a FACS Aria (BD). CD4+IL-7R+CD25-/lo helper T-cells were purified as CXCR5+CCR6+CXCR3-CD161-CCR5- TFH17-cells, CXCR5-CCR6+CXCR3-CD161+ or CCR5+Th17-cells or as CXCR5-CCR6+CXCR3-CD161-CCR5-CCR6SP T-cells RNA was isolated using mirVana Isolation Kit (ThermoFisher) Library construction was performed with the TruSeq RNA Sample Preparation Kit v2 (Illumina)
attributes.tag: Experimental Factor: phenotype
attributes.value: CXCR5+CXCR6+CXCR3-CD161-