title: GSM4600859: set3Δ-1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Yeast strains, WT (MHY10244), set3Δ (MHY10869) and H2B-4KA (MHY10883), were grown in SD-Trp media until reaching mid-log phase (OD600 of 0.8 to 1.0). The 1.0 OD600 equivalent of cells was used for RNA preparation using the RNeasy Mini Kit (Qiagen, 74104). Contaminating DNA was removed from the samples using the DNA-free™ DNA Removal Kit according to the manufacturer's protocol (Ambion, AM1906). RNA samples were submitted to YCGA, and the libraries were constructed by YCGA protocols (https://medicine.yale.edu/keck/ycga/Images/9_tcm240-21599.pdf). The libraries underwent 101-bp paired-end sequencing using an Illumina HiSeq 2500 according to Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4600859