title: GSM3371597: Ctr rep1; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was extracted from islets using TRIzol reagent (Invitrogen , cat. no. 15596018). Poly(A+) RNAs were enriched with biotinylated oligo(dT) and converted to cDNA with Superscript III (Invitrogen, cat. no. 18080-051) A ddNTP was added to the 3'-end of the cDNA by a terminal transferase. After second-strand cDNA synthesis, 23 cycles of PCR were performed to amplify the cDNA with lengths of 200-400 nt
attributes.tag: GEO Accession
attributes.value: GSM3371597