title: GSM3673595: H3K27ac-ChIP-OS2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed at 37°C with 1% paraformaldehyde for 15 min, washed twice in cold phosphate buffered saline and scraped and pelleted at 3000 rpm for 5 min; cell pellets were resuspended in 50 uL lysis buffer, then diluted with 500 uL TE buffer for sonication. 20 ug chromatin, 3 ug antibody, and 11 uL dynabeads were incubated in ChIP buffer at 4°C overnight. The chromatin were eluted by 150 uL elution buffer followed by decrosslinking at 65℃ overnight. After incubation with RNase A and Proteinase K, DNA was precipitated by phenol:chloroform:isoamyl (25:24:1, v/v), dissolved in TE buffer, and stored at -80°C. Librariy preparation and Illumina HiSeq 2000 sequencing were done by the IGM Genomics Core, University of California, San Diego.
attributes.tag: GEO Accession
attributes.value: GSM3673595