title: GSM2805149: Eed null-CJ7 day5 EB rep1; Mus musculus; RNA-Seq
library_construction_protocol: MEF-depleted ES cells were lysed in Trizol and total RNA was isolated using Direct-zol RNA preparation kit (Zymo Research, R2070). On column DNase tretment was done according to manufacturer's protocol. After checking the quality and quantity of the isolated RNA, 0.5 to 1 microgram of total RNA was depleted of rRNA using RiboZero Gold magnetic kit (Illumina Inc. MRZG12324). The rRNA-depleted RNA was used for cDNA generation and library construction. cDNA was generated using Truseq total RNA kit (Illumina Inc.) according to the manufacturer's protocol. End repair, A-tailing, adapter ligation  and PCR amplification was carried out according to manufacturer's instructions with modifications described elsewhere (Bowman et al., BMC Genomics 2013).
attributes.tag: GEO Accession
attributes.value: GSM2805149