title: GSM2881094: Dorset; Ovis aries; MeDIP-Seq
library_construction_protocol: A phenol-chloroform extraction method Genomic DNA mixtures were sonicated to gain fragments in the range 100–500 bp, and dAs were added to the 3' ends of the DNA fragments. The adaptors were then ligated to the two ends of each fragment with paired-end DNA sample prep kit (Illumina) according to the manufacturer's protocol. The double-stranded DNA was denatured to single strands, and then the anti-5-methylcytosine mouse monoclonal antibody (Calbiochem) was used to immunoprecipitate the DNA fragments containing regions of methylated cytosines with magnetic methylated DNA immunoprecipitation kit (Diagenode). The precipitated DNA fragments were then amplified by PCR and separated by agarose gel electrophoresis. The 200–300 bp fragments were excised and extracted using a gel extraction kit (28706; Qiagen). The selected DNA fragments were quantified using an Agilent 2100 Analyzer. The quantified library was sequenced in an Illumina HiSeq 2000 at the BGI (Shenzhen, China). Libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2881094