title: GSM2305491: Control Pravastatin biological replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total cell RNA was prepared from ~8 million cells using Trizol reagent (Sigma) following the manufacturer's protocol. Purified total RNA was quantified by Nano drop and quality checked using the Agilent Bioanalyser. Samples that showed minimal degradation as measured by RNA integrity number (RIN) > 8.0 were further processed for Illumina sequencing with the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina, Part# RS-122-2103). The starting amount of total RNA for all samples was 1ug. Resulting libraries were quantified and quality control checked for size and primer dimer contamination. Libraries with sizes below 500bp and very low (<5%) primer dimer contamination were sequenced on the Illumina HiSeq 4000 with the paired-end 101bp dual indexing protocol. FastQ files were generated using CASAVA BCL to FastQ (version 2.16).
attributes.tag: GEO Accession
attributes.value: GSM2305491