title: GSM2266214: Elav/TDP43:S12; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Fly heads were collected for each genotype and total RNA was purified with Trizol (Invitrogen). RNA-Seq libraries were constructed using the NuGEN Ovation Drosophila RNA-Seq kit including DNase treatmen with HL-dsDNase (ArcticZymes Cat. # 70800-201) and cDNA fragmenation using the Covaris E220 system according to manufacturer recommendations.  After amplification library quality was measured using the Agilent Bioanalyzer system and quantity was determined using Life Technologies Qubit dsDNA HS Assay kit (for use with the Qubit 2.0 Fluorometer).  Prior to sequencing pooled libraries were quantified using the Illumina Library Quantification kit (with Universal qPCR mix) from Kapa Biosystems and a 1.2 pM loading concentration was used for PE101 on the Illumina NextSeq500 platform.
attributes.tag: GEO Accession
attributes.value: GSM2266214