title: GSM3902388: 1 hour concanamycin A treatment [14517X4]; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells were lysed by micro-bead beating and RNA was purified with Qiagen's Rneasy mini purification kit. Contaminating DNA was removed by on column Rnase-free Dnase (Qiagen) treatment. Total RNA samples (100-500 ng) were hybridized with Ribo-Zero Gold rRNA Removal Kit Yeast (Illumina MRZY1306) to substantially deplete rRNA from the samples. Stranded RNA sequencing libraries were prepared as described using the Illumina TruSeq Stranded Total RNA Kit. Purified libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay. The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit. Individual libraries were normalized to 10 nM and equal volumes were pooled in preparation for Illumina sequence analysis.
attributes.tag: GEO Accession
attributes.value: GSM3902388