title: RNA-Seq of MEC1 cells treated with TTNPB or DMSO
design: Total RNA was isolated using TRIzol reagent (Cat # 15596026, Thermo Fisher Scientific, Waltham MA) according to manufacturer s protocol and stored at -80 C. RNA was pretreated with DNA-free  DNA Removal Kit (Cat # AM1906 Invitrogen). RNA was purified from of eight samples, consisting of four biological replicates for each DMSO or TTNPB treatment, and quantified by fluorometry using Qubit (Thermo Fisher Scientific). RNA integrity (RIN) was measured using an Agilent 2200 TapeStation system (Agilent, Santa Clara, CA). The RNA was subjected to polyA selection before library construction