title: GSM4260803: LKR10 shMLL4-1-rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared from shControl (shLuc) and MLL4-knockdown (shMLL4-1 and shMLL4-3) LKR-10 cells using Trizol reagent (Life Technologies). Briefly, to isolate total RNA from shLUC, shMLL4-1 and shMLL4-3 infected LKR-10 cells, first the cells were selected in 1ug/ml puromycin containing medium and were harvested at 70-80% of confluency. Total RNA was isolated using Trizol reagent (Life Technologies).  RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4260803