title: GSM3138106: Stem Total RNA - Sample 3; Homo sapiens; RNA-Seq
library_construction_protocol: For MeRIP-seq we IP methylated RNA using a m6A specific antibody and isolated RNA from the immunoprecipitated fragments. RNA for RNA-seq was isolated with a classic Trizol extraction. For Ribo-seq we performed a sucrose gradient extraction of ribosomes and purification of ribosome-bound RNA. In all instances rRNA was depleted before library construction. Libraries were constructed according to standard Illumina protocols. All libraries were constructed by Genewiz, the Epigenomic Core at Einstein Medical School and the Genomic Core at Tufts University.
attributes.tag: GEO Accession
attributes.value: GSM3138106