title: GSM3731563: Neil2_Mo_rep1; Xenopus laevis; RNA-Seq
library_construction_protocol: For each replicate sample a pool of 6 embryos at stage 23 was collected. Total RNA was extracted with Trizol according to the manufacturer's instructions. NGS libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina) according to the manufacturer's recommendations and amplified in 12 PCR cycles. Libraries were profiled in a High Sensitivity DNA chip on a 2100 Bioanalyzer and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life Technologies). The NGS libraries were sequenced on a HiSeq 2000 Illumina sequencer, for 51 cycles plus seven cycles for the index read.
attributes.tag: GEO Accession
attributes.value: GSM3731563