title: GSM2806748: Lgr5+Id2:tdTomato-_E13.5_intestinal epitheliua cells labelled at E9.5_wt_rep3; Mus musculus; RNA-Seq
library_construction_protocol: 500 embryonic intestinal epithelial cells were isolated by FACS directly in 7ml of lysis buffer (Clontech) supplemented with 5% RNase inhibitor and stored at -80°C. For each replicate, RNA was isolated from pull of three embryos (E13.5) or single embryos (E15.5). PolyA+ mRNA was used for cDNA synthesis using SMARTer v3.0 kit (Clontech) according to manufacturer’s instructions. Amplification was performed for 15 cycles. After cDNA fragmentation (Covaris), libraries were prepared using Ovation Ultralow Library System (NuGEN) according to manufacturer’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2806748