title: GSM4658257: ParE3 t0 rep2; synthetic construct; OTHER
library_construction_protocol: Plasmids were extracted using the ZR Plasmid Miniprep - Classic kit (Zymogen) The entire ParD3 gene was used as a template for PCR (12 cycles) with custom barcoded primers containing Illumina flowcell adapator sequences. To increase the diversity of the amplicon pool, the PCR also incorporated 4-6 random nucleotides to the 5' end of each ParD3 gene.
attributes.tag: GEO Accession
attributes.value: GSM4658257