title: GSM4158694: HCT116_C_offBead; Homo sapiens; OTHER
library_construction_protocol: After we isolated the nuclei from cultured cell or tissue section, the nuclei was labeling with Nt.CviPII, Biotinylated 5mC-dNTP mixture, decroslinked for O/N at 65 degree, and then genomic DNA was extracted using phenol-chloroform method. Biotin-labeled open chromatin DNA was captured by streptavidin bead and the beads were washed with high salt buffer for 4 times, and followed the library preparation procedure using NEBNext Ultra II DNA library Prep Kit (E7370S, New England Biolabs, Inc)
attributes.tag: GEO Accession
attributes.value: GSM4158694