title: SDB 16S Amplicon
design: The 16S rRNA gene PCR was performed using the forward primer 27F (5’-AGAGTTTGATCMTGGCTCAG-3’) and the reverse primer 338R (5’-TGCTGCCTCCCGTAGGAGT-3’) for bacteria, and primers A8F (5’-TCCGGTTGATCCTGCC-3’) and A344R (5’-TCGCGCCTGCTGCICCCCGT-3’) for archaea (Nakatsu & Marsh, 2007).  All primers included 5’ Titanium Fusion adapter sequences (forward primer A-tag: CCATCTCATCCCTGCGTGTCTCCGACTCAG; reverse primer B-tag: CCTATCCCCTGTGTGCCTTGGCAGTCTCAG) as well as a unique 8-nucleotide barcode tag in the reverse primer as previously described (Hamady et al., 2008)