title: GSM4407718: CRISPR screen_ER stress-UPR pathway; Homo sapiens; OTHER
library_construction_protocol: trypsin treat and count the cells, extract the gDNA using DNAzol from Invitrogen, PCR amplify according to Broad GPP sgRNA PCR protocol Amplification of gRNA sequence from isolated gDNA simultaneously labeled each sample with a pooled P5 primer and unique P7 primer. The P5 primer includes the illumina flow cell attachment sequence, illumina sequencing primer, vector binding sequence, and a stagger region. The P7 primer includes the illumina flow cell attachment sequence, illumina sequencing primer, vector binding sequence and an 8 base pair UMI unique to each individual primer. Libraries from individual samples, each labeled with a unique P7 primer, were pooled and mixed with 10% PhiX Sequencing Control (Illumina) and sequenced on the Illumina Nextseq 500.
attributes.tag: GEO Accession
attributes.value: GSM4407718