title: GSM4156857: P1_d2_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy Micro Kit (Qiagen). RNA quality was assessed using an Agilent 2100 Bioanalyzer. Ribosomal RNA was removed using NEBNext rRNA Depletion Kit. 5 ng total RNA was used for library preparation with the 'SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian' (Takara). Libraries were barcoded by a dual indexing approach and were amplified by 11 PCR cycles. Fragment length distribution was monitored using 'Bioanalyzer High Sensitivity DNA Assay' (Agilent). Quantification was performed with the 'Qubit® dsDNA HS Assay Kit' (ThermoFisher). The combined library pools were denatured with sodium hydroxide and were finally diluted to 1.5 pM according to the Denature and Dilute Libraries Guide (Document # 15048776 v02; Illumina, San Diego, USA). 1.3 mL of each denatured pool was loaded on an Illumina NextSeq 550 sequencer using a High Output Flowcell for 75 bp single reads (#FC-404-2005; Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4156857