title: Radish Silty Clay Loam DA Manure
design: Greenhouse radish taproots and lettuce leaves were grown to maturity, and surface bacterial cells were dislodged into solution (0.1% each peptone & Tween 80). Cells were collected on 0.22-m mixed cellulose membranes for DNA extraction (MPBio FastDNA Spin Kit for Soil, Zymo PCR inhibitor clean-up kit).  Accel-NGS 2S Plus DNA Library Kit was used for library prep, 14 PCR cycles.