title: GSM2983886: ∆treR galactose replicate 2; Streptococcus mutans; RNA-Seq
library_construction_protocol: RNA extraction was performed using the Qiagen RNeasy Power Microbiome Kit according to the manufacturer's instructions, mRNA was purified using the Zymo RNA Clean and Concentrator Kit cDNA libraries from rRNA-depleted RNA were generated using random-primed cDNA synthesis methods according to the ScriptSeq v2 RNASeq Library preparation protocol (Epicentre, Madison, WI).  Prior to second strand cDNA synthesis, the di-tagged cDNA was purified using the Agencourt AMPure XP system (Beckman Coulter, Brea, CA).  ScriptSeq index primers were added to the libraries which were then PCR amplified for 15 cycles.
attributes.tag: GEO Accession
attributes.value: GSM2983886