title: Genome-wide locatlization analyses of Rad21 (1h LG268, Rep1) binding sites
design: Libraries were prepared with the Nugen Ultralow Library System from at least two biological replicates per manufacturer’s instructions. Briefly, 1ng immunoprecipitated DNA were submitted to End-repair, then adaptors were ligated to double stranded DNA fragments. Adaptor containing fragments were PCR amplified in 18 cycles. Libraries were gel extracted and analyzed by Agilent 1000 DNA chip. Libraries were sequenced on HiScanSQ according to manufacturer’s protocol.