title: GSM4041642: RKN susceptable control; Gossypium hirsutum; RNA-Seq
library_construction_protocol: Whole intact root system from inoculated and un-inoculated M120 and C201 plants were carefully removed from the potting-mix at 12 and 30 DAI, washed with de-ionized water, dried with sterilized paper towels and immediately frozen in liquid nitrogen. Frozen roots were stored at -80 °C until RNA extraction All libraries were constructed using the NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs Inc.) following the manufacturers protocol.  The NEBNext Multiplex Oligos for Illumina (Index Primers Set 2, New England BioLabs Inc.) containing adaptors and primers were used to label libraries facilitating pooling.