title: GSM2879401: N2tbooh3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted with Trizol ribosomal RNA removed using the RiboGone - Mammalian—Low Input Ribosomal RNA Removal Kit for Human, Mouse and Rat Samples (Takara #634847). Ribosomal RNA depletion and RNA integrity were verified on a Bioanalyzer with the RNA 6000 Pico kit (Agilent #5067-1513). We constructed cDNA libraries with the SMARTer Universal Low Input RNA Kit (Takara #634900), and measured adapter-ligated concentrations with the High Sensitivity DNA Analysis Kit (Agilent #5067-4626) and by qPCR standard-curve quantitation using the KAPA Library Quantification Kit Illumina Platforms (KK4824). RNA-seq was done on an Illumina Nextseq 500 using 76bp x2 paired-end reads, using the NextSeq 500/550 High Output Kit v2 (150 cycles).
attributes.tag: GEO Accession
attributes.value: GSM2879401