title: GSM4658498: naïve replicate 2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cultures were pelleted in large table-top centrifuge with swing-bucket rotor at 4300 x g at 4°C for 20 minutes, washing once with ice-cold 1X TBS. RNA was harvested as described in (Carlile et al., 2015). Total RNA was treated with RiboZero (Illumina, San Diego, CA) to remove most rRNAs but retain mRNAs and other ncRNAs. RNA was fragmented and submitted for Illumina sequencing Library preparation and Illumina sequencing was performed at the Beijing Genomics Institute in late 2017.
attributes.tag: GEO Accession
attributes.value: GSM4658498