title: GSM2724421: Jugular neurons_1 (Capsaicin selection); Mus musculus; RNA-Seq
library_construction_protocol: From each jugular/nodose ganglion complex of the mouse, the most rostral (jugular) and the most caudal (nodose) portion were used. The ganglia were enzymatically dissociated. Individual capsaicin-sensitive (capsaicin selection) /Trpv1-positive (Trpv1-tdTomato selection) nodose and jugular neurons were collected into the pipette under the microscope. cDNA synthesis was performed using SMARTer Ultra Low Input RNA Kit v3 following manufacturer’s protocol (Clontech). Cells were used directly as starting material without RNA extraction to avoid extra loss of RNA. Illumina paired-end DNA sequencing library was generated using Low Input Library Prep Kit v2 following manufacturer’s protocol (Clontech)
attributes.tag: GEO Accession
attributes.value: GSM2724421