title: Hypoxia-Mono-Oligosome
design: Normoxic (21% O2) and hypoxic (1% O2, 24 hr) U87MG human glioblastoma cells were subjected to polysome fractionation. Total RNA were isolated from individual fractions by standard phenol/chloroform extraction and ethanol precipitation following proteinase K treatment. Equal volumes of individual fractions from four independent experiments were pooled to yield the monosome/oligosomes (MO) and polysome (P) samples.