title: MinION sequencing
library_construction_protocol: PrePCR treated and SQK-MAP004 used.
attributes.tag: kit
attributes.value: SQK-MAP004
attributes.tag: sequencing protocol
attributes.value: gDNA
attributes.tag: dna extraction protocol
attributes.value: Tri-reagent extraction of total RNA followed by full length cDNA sysnthesis and targeted amplification of the cca-1 gene by PCR
attributes.tag: call date
attributes.value: 41978
attributes.tag: sequencing date
attributes.value: 41974
attributes.tag: reloading of reagents
attributes.value: 6ul at 0, 5 and 21hrs
attributes.tag: pre-sequencing mix dna quantification
attributes.value: 9.62ng/ul by Qbit