title: RNA-seq for maize: V4 leaf
design: The total RNA was isolated using Trizol reagent (Invitrogen) and a total amount of 3 g RNA per sample was used as input material for qRT-PCR and RNA sequencing. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. The library preparations were sequenced on an Illumina Hiseq platform and 125 bp/158 bp paired-end reads were generated at Novogene (Beijing).