title: GSM2308262: PEG group; Triticum aestivum; RNA-Seq
library_construction_protocol: Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA from each sample was used to prepare the circRNA sequencing library, which included the following steps: 1) 5 μg total RNA were pretreated to enrich circRNA using CircRNA Enrichment Kit (Cloud-seq Inc, USA). RNA libraries were constructed by using pretreated RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).
attributes.tag: GEO Accession
attributes.value: GSM2308262