title: GSM3672570: 5-2607-C; Homo sapiens; RNA-Seq
library_construction_protocol: Add 0.25 mL of TRIzol™ Reagent (Thermo Fisher Scientific, Cat#15596026) per 24-well well directly to the culture dish to lyse the cells. Transfer the lysate to 1.5 mL RNase-free eppendorf tube. Add 50 µL of chloroform in the hood, and then securely cap the tube. Invert 2-3 times and vortex 5-10 sec. Hold on ice for 10 min, inverting every 3 min. Centrifuge the sample for 15 minutes at 12,000× g at 4°C. Transfer clear supernatant to fresh RNase-free tubes with 150 µL of Isopropanol. Centrifuge the sample for 10 minutes at 12,000× g at 4°C. Discard the supernatant with a micropipettor. The pellet is washed in 0.5 mL of ice cold 70% ethanol from -20°C. Resuspend the pellet in 50 μL of RNase-free water. Store the RNA at -70°C. RNA sequencing libraries were prepared using the Kapa Total RNA library prep kit. NovaSeq, 100nt, Paired-End Read
attributes.tag: GEO Accession
attributes.value: GSM3672570