title: 16S rDNA library from Nahant Canoe Cove: 63 um fraction
design: V4 variable region of the 16S rDNA from water collected through a plankton net (63um) in a Oakridge tube and concentrated on a 0.2 um filter. V4 amplified with U515 and E786R with a two step PCR method, and identified in a multiplexed sampled by a sample spcecific barcode