title: GSM3591875: HNAD2-AS1 ChIRP rep1; Homo sapiens; OTHER
library_construction_protocol: Chromatin isolation by RNA purification (ChIRP) was performed as described previously.In brief, human liver cells were cross-linked using glutaraldehyde. After glycine quenching, the nuclear lysate was sonicated for 25–30 cycles, 30 s on 30 s off at 4 °C, with BioRuptor twin sonicator (Diagenode). HAND2-AS1 and LacZ pulldown probes with BiotinTEG were designed by Biosearch Tecnologies and allowed to hybridize overnight with sonicated chromatin at 37°C (100 pmol probe per 1 ml chromatin). After hybridization, C1 Dynabeads (Life Technologies) were added and incubated for 30 min. Strictly follow protocols from NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® ChIRP-seq
attributes.tag: GEO Accession
attributes.value: GSM3591875