title: GSM3362388: Wassilewskija; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Tri-Reagent (Molecular Research Center, Inc., USA)  extraction of total RNA was performed according to the manufacturer's instructions. BS-Seq: About 500 ng of genomic DNA was isolated from various honey bee developmental stages, fragmented by sonication, end repaired, and ligated to custom synthesized methylated adapters (Eurofins MWG Operon) according to the manufacturer's (Illumina) instructions for gDNA library construction. Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (QIAGEN), as outlined in the manufacturer's instructions. The bisulfite-converted libraries were then amplified by PCR using ExTaq DNA polymerase (Takara Bio) for 12 cycles. The enriched libraries were purified using the solid-phase reversible immobilization method using AM-Pure beads (Beckman Coulter) prior to quantification with a Bioanalyzer (Agilent). Sequencing performed on the Illumina HiSeq 2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM3362388