title: GSM2305451: H3PanAc_Adult_ChIP; Danio rerio; ChIP-Seq
library_construction_protocol: Lysates were clarified from micrococcal nuclease treated erythrocytes nuclei and histone-DNA complexes were isolated with antibody. The ChIP-seq libraries were prepared with the Hyper Library Construction Kit (Kapa Biosystems) according to the manufacturer's protocol. The libraries were pooled in equimolar concentration, quantitated by qPCR and sequenced on one lane for 101 cycles from one end of the fragments on a HiSeq2500 using a HiSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina). As a result 210705238 reads were obtained and used in the downstream analysis.
attributes.tag: GEO Accession
attributes.value: GSM2305451