title: GSM2981763: Th0 Blimp1 rep2; Mus musculus; RNA-Seq
library_construction_protocol: Approximately 0.5e6 cells were isolated by sorting GFP+ cells from three independent biological replicates of CD4 T cells transduced with Empty Vector or Blimp-1 expressing retrovirus. Total RNA was prepared by using mirVana miRNA Isolation Kit (AM1560, ABI). A fraction of total RNA (2 μg) was processed into mRNA-seq library by using TruSeq RNA sample prep kit (FC-122-1001, Illumina). The libraries were sequenced for 50 cycles (single read) with a HiSeq 2000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2981763