title: GSM3673729: Mouse_IVD_Col2Cre_Control_RNAseq_replicaC3; Mus musculus; RNA-Seq
library_construction_protocol: Intervertebral discs from the thoracic and lumbar spine (T8-L5) of the 20-day-old Col2Cre; Adgrg6f/f and wild type control mice were isolated in cold PBS, snap frozen in liquid N2 and pulverized in liquid nitrogen. Each biological replicate contains itervertebral discs isolated from 2-3 mice.Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analyzed on Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). RNA fragments were reverse-transcribed to create the final cDNA libraries following the NEBNext® Ultra™ RNA Library Prep Kit (Illumina, San Diego, USA).
attributes.tag: GEO Accession
attributes.value: GSM3673729