title: siRNA-seq of WT Arabidopsis thaliana: biological replicate 2
design: The five-day-old A. thaliana etiolated seedling samples stored at 80C, as described above, were ground into powder using liquid nitrogen. Total RNA was extracted using mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA) following the manufacturer's instructions. The integrity analysis was performed on a Bioanalyzer by the Beijing Genomics Institute (BGI), Shenzhen, China, which also performed the library construction according to standard protocols.