title: GSM4259759: TWH; Triticum turgidum subsp. durum; miRNA-Seq
library_construction_protocol: Total RNA was extracted from young seedlings using Tri reagent (Sigma-Aldrich) and treated with TURBO DNase (ThermoFisher Scientific) following the manufacturer's instructions. The concentration and quality of RNA samples were measured on a NanoDrop Lite spectrophotometer (ThermoFisher Scientific). RNA integrity was assessed by agarose gel electrophoresis and a Bioanalyzer (RIN > 9). Total RNA from eight biological replicates in each group were pooled for small RNA sequencing. Four small RNA libraries (TCG, TWH, SCG, SWH) were constructed using the TruSeq Small RNA Sample Prep Kit from the four groups. Single-end sequencing (50bp) was performed on an Illumina HiSeq2500 by LC-Bio (Hangzhou, China).
attributes.tag: GEO Accession
attributes.value: GSM4259759