title: GSM2550628: Patient 6 Tumor 1; Homo sapiens; OTHER
library_construction_protocol: UV crosslinked pulverized tissue was lysed in 1x PXL buffer, lysates were Dnase treated, partially RnaseI digested, clarified by centrifugation, and Argonaute-mRNA complexes isolated with pan-Ago 2A8 antibody. CLIP libraries were prepared as in Luna, JM et al (2015). 1st PCR Products were amplified in a second round of PCR that added a 4 nt index (for multiplexing) and a 16 nt common primer sequence. Raw files are demultiplexed on the basis of this index, but have not been further modified. CLIPseq
attributes.tag: GEO Accession
attributes.value: GSM2550628