title: GSM4483243: C_G10; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Mini Kit (Qiagen) following manufacturer's instructions. The resulting RNA was submitted for library preparation by the Wellcome-MRC Cambridge Stem Cell Institute. Initial QC was performed using Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and TapeStation Analysis software (Agilent Technologies). 600 ng RNA was used for library preparation using the NEBNext Ultra8482 II Directional RNA Library Prep Kit (Illumina) and the QIAseq FastSelect RNA Removal Kit (Qiagen). Libraries were subsequently measured using the Qubit system and visualised on a TapeStation D5000.
attributes.tag: GEO Accession
attributes.value: GSM4483243