title: GSM4310253: Young 1 Pre-differentiation; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspensions were obtained from limb muscle tissue after mechanical digestion with scissors and enzymatic digestion with Collagenase II and Dispase. Suspensions were filtered through a 40 um filter before sorting for live cells via FACS. The populations of muscle stem cells and fibro/adipose progenitors were enriched by FACS to capture more of these rare cells. After differentiation in vitro, muscle stem cells and fibro/adipose progenitors were dissociated from culture wells and libraries were prepared from single cell suspensions. Libraries were constructed following the 10X Genomics 3' Single Cell transcriptome v3 protocol.
attributes.tag: GEO Accession
attributes.value: GSM4310253