title: GSM3213852: 70-2_S16; Mus musculus; RNA-Seq
library_construction_protocol: AUP-17-38 Total RNAs from the hippocampus were extracted by using RNeasy Mini Kit (Qiagen). RNA integrity number (RIN) was measured by Agilent 2100 Bioanalyzer and samples with RIN >= 8.0 were kept for cDNA synthesis.  cDNA synthesis and amplification were performed followed by Smart-seq2 (1) standard protocol.  Libraries were constructed by using the Nextera DNA Sample Preparation Kit (Illumina). Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Illumina). The libraries were sequenced using paired-end 43bp mode on Illumina NextSeq500 platform with around 10 million reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM3213852