title: GSM2728794: 14d_sample_2; Bacillus subtilis; RNA-Seq
library_construction_protocol: To isolate RNA, cell pellets were flash frozen in liquid nitrogen immediately after harvesting and stored at −80°C prior to RNA extraction. Frozen pellets were grounded by using a mortar and pestle before immersion in QIAzol Lysis Reagent (Qiagen). RNA was isolated and purified using the RNeasy kit (Qiagen), including an on-column treatment with the RNase-free DNase set (Qiagen). The concentration was measured on a NanoDrop ND-2000 (Thermo Scientific) and RNA integrity was examined using the 2200 TapeStation System with Agilent RNA ScreenTapes (Agilent Technologies). A ribosomal RNA depletion was performed using the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Illumnia). Bar-coded RNA libraries were generated according to the manufacturers’ protocols using the Ion Total RNA-Seq Kit v2 and the Ion Xpress RNA-Seq barcoding kit (Thermo Fisher Scientific). The size distribution and yield of the barcoded libraries were assessed using the 2200 TapeStation System with Agilent D1000 ScreenTapes (Agilent Technologies). Sequencing was performed on the Ion Proton system using the Ion PI Chips (Thermo Fisher Scientific) according to the manufacturers’ protocols.
attributes.tag: GEO Accession
attributes.value: GSM2728794