title: 18S amplicon sequencing of SIO pier seawater enriched with Synechococcus sp. WH9311 on 7/29/14 and incubated for 5 days (replicate 2)
design: Cultured representative of Synechococcus added to 600 ml seawater at concentration of 10^6 cells/ml; after five days of incubation, 250 ml filtered on 0.2 micron Supor filter; total DNA extracted using phenol-chloroform extraction and Qiagen DNEasy Blood and Tissue kit; sent to Research and Testing Laboratory (Lubbock, TX) for sequencing of 18S amplicon using TAReuk primers (Stoeck 2010)