title: GSM3670774: 5'Tag-seq  Ctrl_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cultured alveolar epithelial cells were first stained with APC conjucated anti-human LNGFR antibody. Cells were next stained with anti-APC microbeads (Miltenyi). Labeled cells were magnetically sorted twice. Whole transcript amplification for cultured alveolar epithelial type II cells was performed using 5'Tag-seq method according to a previous report with modifications (Shichino et al., 2019). 5' regions of amplified cDNA were enriched using NEBNext UltraII FS library prep kit (New England Biolabs) according to the manufacturer's protocol except using CS1 adapter, trP1 primer, and IonA-BC[N]-CS1-primer for adding sequencing adapters and barcodes. Library concentration was quantified by using the KAPA Library Quantification Kit for Ion Torrent (KAPA Biosystems) according to the manufacture's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3670774