title: GSM3361856: AB4845; Mus musculus; RNA-Seq
library_construction_protocol: Mice were perfused by injection of cold PBS via the right ventricle prior to lung dissection. Lung tissue was dissected from mice and half tissues were homogenized using lung dissociation kit (Miltenyi Biotec), while the second half of the lung was dissociated with Eastase. Single cell suspension was stained with Flow cytometry antibodies. Murine thymi were treated with enzymes cocktail (Collagenase, Dispase and Dnase) at 37C. Cells were separated by Percoll gradient density centrifugation, filtered and stained with Flow cytometry antibodies. Murine spleens  were treated with acccutase, filtered and stained with Flow cytometry antibodies. Murine livers  were treated with liberase, filtered and stained with Flow cytometry antibodies. Murine tumors  were treated with colagenase, filtered and stained with Flow cytometry antibodies. 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014) single cell RNA-seq for gene expression quantitation
attributes.tag: GEO Accession
attributes.value: GSM3361856