title: GSM4602141: Leaves, Col-0, 23C, mock Rep 3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: 20 to 60 mg of fresh leaf tissues were flash-frozen in liquid nitrogen and ground using a TissueLyser (Qiagen). Plant RNA was extracted using a Qiagen Plant RNeasy Mini Kit following the manufacturer's protocol, including the recommended on-column DNase I digestion. Three RNA samples with the best scores (RIN score >= 7) were pooled on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced in a 1x 50-bp single-end format using Rapid SBS reagents.
attributes.tag: GEO Accession
attributes.value: GSM4602141