title: GSM2640951: Ntg_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted by miRNeasy kit (QIAGEN), RNA quality determined with 6000-Nano Bioanalyzer (Agilent, Santa Clara, CA.), and samples with RIN values ranged between 8 and 8.9 were used Sequencing-compatible poly A-terminated single-end libraries were generated using an RNA Library prep kit (NEBNext® Multiplex, E7330S, New England Biolabs) following manufacturer’s instructions, with 12 amplification rounds poly A-terminated single-end libraries: Libraries were sequenced on a NextSeq Series Sequencing System (The HUJ Center for Genomic Technologies) using two Illumina chips (Illumina 500® NextSeq High Output v2 Kit, FC-404-2005, Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2640951