title: Microbiota results obtained from water sampled at location A in October, replicate 3
design: The protocol utilized a two-step micelle PCR (micPCR) protocol. In the first step, micPCR was performed using modified 515F and 806R primers that amplified the V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. During the second round of amplification, dual indices and Illumina sequencing adapters were attached using the Nextera KT Index kit (Illumina). Paired-end sequencing of the 16S rRNA gene amplicon library was performed using the MiniSeq system (Illumina).