title: GSM4106381: mESC_RNAseq rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq (converted or not) nuclei were prepared from 50,000 cells for each transposition reaction. For ChIP-seq (converted or not), Lysates were clarified from sonicated nuclei and KLF4-bound chromatin was isolated with KLF4 antibody. For ATAC-seq and ChIP-seq, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. For bisulfite converted samples, a transposome was assembled with methylated adaptors for transposition reaction. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4106381