title: RNA-Seq analysis of H1 embryonic stem cell line (HS1272)
design: RNA-Seq analysis of H1 embryonic stem cell line (HS1272) using Illumina Genome Analyzer IIx
library_construction_protocol: Refer to document 'Strand Specific 96-well Library Construction for Illumina Sequencing' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: EXPERIMENT_TYPE
attributes.value: mRNA-Seq
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: Total RNA was extracted using Trizol from Invitrogen as per manufacturer's instructions.
attributes.tag: LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
attributes.value: Phusion
attributes.tag: LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
attributes.value: 98C 30 sec, 10 cycle of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold
attributes.tag: LIBRARY_GENERATION_PCR_NUMBER_CYCLES
attributes.value: 10
attributes.tag: LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
attributes.value: AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
attributes.tag: LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
attributes.value: CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
attributes.tag: LIBRARY_GENERATION_PCR_PRIMER_CONC
attributes.value: 0.5 uM
attributes.tag: LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
attributes.value: 8% Novex TBE PAGE gel purification
attributes.tag: RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE
attributes.value: NNNNNN
attributes.tag: RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL
attributes.value: Invitrogen Superscript II RT
attributes.tag: LIBRARY_GENERATION_PCR_TEMPLATE
attributes.value: cDNA
attributes.tag: EXTRACTION_PROTOCOL_MRNA_ENRICHMENT
attributes.value: Purification of polyA+ mRNA using MultiMACS 96 Separation Unit
attributes.tag: RNA_PREPARATION_INITIAL_RNA_QLTY
attributes.value: RIN 9.9
attributes.tag: RNA_PREPARATION_INITIAL_RNA_QNTY
attributes.value: 20000 ng
attributes.tag: LIBRARY_FRAGMENTATION
attributes.value: COVARIS E210
attributes.tag: LIBRARY_FRAGMENT_SIZE_RANGE
attributes.value: 270-500 bp