title: GSM2109692: N2_mock(RNAi)_L1toL4_20°C_rep1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Worms were harvested, washed three times with M9 medium, resuspended in 1 ml of TRI Reagent (Molecular Research Center, Cincinnati, OH, USA) and frozen in liquid nitrogen. Worms were broken open by five repeats of freeze and thaw, and RNA was extracted and purified according to the supplier's protocol with the modification that RNA was incubated with 50% 2-propanol at −80°C overnight for efficient precipitation. The purified RNA was treated with DNA-free Kit (Thermo Fischer Scientific, Waltham, MA, USA) to remove DNA. Libraries were prepared using the TruSeq Standard mRNA Library Prep Kit (Illumina, San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM2109692