title: GSM4106399: X4977_KirbyE_DGM2R_V1N_1_S12_L001; Mus musculus; RNA-Seq
library_construction_protocol: Approximately 30,000 cells from two female and two male adult NSPCs cultures were harvested with accutase and washed with PBS before RNA isolation with the Clontech Nucleospin RNA XS Plus isolation kit (Takara 740990.10) per manufacturer protocol. RNA was also isolated from whole DGs isolated from one male and one female C57/Bl6 mouse with the same kit. RNA quality and quantity was assessed with a Qubit RNA HS assay kit (Invitrogen). All cultured samples used had a RIN value of 10 while whole DG samples had RIN values over 8. Libraries were generated with the NEBNext Ultra II Directional RNA Library prep kit (Illumina) for the whole DGs and the Clontech HT kit for cultured NSPCs. Purified library products were then used in HiSeq 4000 paired-end sequencing (Illumina) to a depth of 15 - 20 million 2x150 bp clusters. Libraries were generated with the NEBNext Ultra II Directional RNA Library prep kit (New England Biolabs) for the whole DGs and the Clontech SMART/Seq HT kit (Takara) for cultured NSPCs. Purified library products were then used in HiSeq 4000 paired-end sequencing (Illumina) to a depth of 15 - 20 million 2x150 bp clusters.
attributes.tag: GEO Accession
attributes.value: GSM4106399