title: GSM3670741: L465-ATAC-Diamide-0_min-rep2; Saccharomyces cerevisiae; ATAC-seq
library_construction_protocol: ATAC-seq was carried out on the same nuclei isolated for SMAC-seq (before resuspension in M.CviPI Reaction Buffer), by resuspending nuclei with 25 uL 2X TD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% Dimethyl Formamide), 2.5 uL transposase (custom produced) and 22.5 uL nuclease-free H2O, and incubating at 37C for 30 min in a Thermomixer at 1000 RPM. Transposed DNA was isolated using the DNA Clean & Concentrator Kit (Zymo, cat # D4014) and PCR amplified. Libraries were then sequenced on a Illumina NextSeq instrument as 2x36mers or as 2x75mers. ATAC-seq was carried out on the same nuclei isolated for SMAC-seq (before resuspension in M.CviPI Reaction Buffer), by resuspending nuclei with 25 uL 2X TD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% Dimethyl Formamide), 2.5 uL transposase (custom produced) and 22.5 uL nuclease-free H2O, and incubating at 37C for 30 min in a Thermomixer at 1000 RPM. Transposed DNA was isolated using the DNA Clean & Concentrator Kit (Zymo, cat # D4014) and PCR amplified. Libraries were then sequenced on a Illumina NextSeq instrument as 2x36mers or as 2x75mers.
attributes.tag: GEO Accession
attributes.value: GSM3670741