title: RNA-Seq of Carcharodon carcharias muscle tissue
design: RNA was isolated from sample using the Zymo Research Direct-zol kit. Total RNA was examined for RNA Integrity Numbers and all samples had values >7. To generate paired-end mRNA sequencing libraries, we used the Illumina mRNA TruSeq kit, which included a poly-A selection module, and sequenced libraries on an Illumina 4000 at the Stanford University Functional Genomics Facility. All raw read files were trimmed and process for paired reads only using default parameters for Trimmomatic v0.36.