title: 16s rRNA V3-V4 METAGENOMIC  SEQUENCING OF VETIVER SOIL
design: DNA were extracted from soil using Dneasy power soil kit, Qiagen. The DNA quality was checked using Qubit DNA assay kit and amplified by PCR. The final product was used for Illumina library preparation.  16S rRNA V3-V4 amplicon libraries were prepared for sequencing using standard  Illumina protocol.  Illumina bcl2fastq Conversion Software v2.17.1.14 used for base calling.Paired-End Stiching using FLASH. OTU Picking, filtering and finding representative sequence using QIIME. Taxonomy Classification based on SILVA database. Alpha Diversity within sample AND RAREFACTION CURVES using QIIME. Beta diversity between samples were also calculated.