title: GSM2108857: M02298; unidentified herpesvirus; RNA-Seq
library_construction_protocol: Salivary gland tissue (parotide gland) were collected and frozen at necropsy.Frozen salivary gland tissue specimens were minced with a sterile razor blade before addition of Trizol. Following a 30 min incubation on ice, total RNA was isolated using a Direct-zol RNA mini prep column (Zymor research) following the manufacturer’s protocol. RNA samples were further purified on “Clean and Concentrate” columns (Zymo research) according to the manufacturer’s instructions. Total RNA integrity was checked using an Agilent 2200 TapeStation (Agilent Technologies) and quantified using a Trinean DropSense96 spectrophotometer (Caliper Life Sciences). RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep Kit (Illumina, Inc.) and a Sciclone  NGSx Workstation (PerkinElmer).  Library size distributions were validated using an Agilent 2200 TapeStation (Agilent Technologies).  Additional library QC, blending of pooled indexed libraries, and cluster optimization was performed using Life Technology’s Invitrogen Qubit® 2.0 Fluorometer (Life Technologies-Invitrogen). RNA seq on poly A selected RNA
attributes.tag: GEO Accession
attributes.value: GSM2108857