title: GSM4159020: DMSO 1 h agb12; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Each Arabidopsis seedling sample was ground to a fine powder and 500 µL of Hot Borate Buffer (200mM sodium tetraborate decahydrate - Sigma-Aldrich, 30 mM EGTA - Sigma-Aldrich, 1% SDS - Fisher, 1% deoxycholic acid - Sigma-Aldrich, 2% polyvinylpyrrolidone (PVP) 40K - Sigma-Aldrich, 0.5% IGEPAL CA-630 - Sigma-Aldrich, 10 mM DTT - Sigma-Aldrich) and 17.5 µL of 20 mg/mL Proteinase K (ThermoFisher) were added. After mixing, the sample and buffer solution were added to a lilac shredder column from a RNeasy Plant Mini Kit (Qiagen). The manufacturer's protocol from the RNeasy Plant Mini Kit was followed for the remainder of the extraction. Poly(A) enrichment and library preparation were performed using the TruSeq Stranded mRNA prep kit (Illumina). Libraries were quantified by fluorometry, immobilized, and processed onto a flow cell, followed by 2 x 75 bp sequencing-by-synthesis on a Next-Seq 500 system (Illumina) with two mid-output cassettes (Illumina). Library construction and RNA sequencing were performed by the UNT Genomics Center (http://untgenomicscenter.squarespace.com/).
attributes.tag: GEO Accession
attributes.value: GSM4159020