title: lung microbiome from sputum for 71B_145_4.1_Exacerbation
design: DNA sequencing of 16S rRNA V3-V5 region using primers 517F (5’-GCCAGCAGCCGCGGTAA-3’) and the reverse primer (5’-CTTGTGCGGGYCCCCGTCAAT-3’) with barcode ATCAGACACG for subject 145 at a Exacerbation visit on 05_24_10