title: GSM4105980: scaRNA-seq Mouse Wildtype fetal liver cells late erythroid (24h) replicate 3; Mus musculus; ncRNA-Seq
library_construction_protocol: isolation protocol: 5x10^6 cells were aliquoted per replicate, pelleted by centrifugation (500g, 5min, R.T) and washed once in 5mL 1xPBS before lysis in TRI Reagent (Merck) by repeated pipetting. Samples were snap frozen and transferred to storage at -80°C until use. Samples in TRI reagent were thawed on ice before extraction according to the manufacturers protocol. Samples were Dnase treated for 30mins at 37C using Turbe DNase before phenol chloroforom extraction and ethanol precipitation. RNA <=300nt was size selected using a 10% denatuiring poly arcylamide gel. RNA was extracted and subjected to a modified veriosn of the NEB Next Small RNa for Illumna library rep kit (NEB), wherin after 3' adapter ligation matierla is phosphatase treated and decapped before continuing with 5' adapter ;igation and the remained opf the protocol according to the manufactureers protocol.
attributes.tag: GEO Accession
attributes.value: GSM4105980