title: GSM3213954: WT_72h_RNA-seq_Rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from naïve and activated B cells with Trizol (ThermoFisher) and the integrity of the RNA was accessed with TapeStation RNA Analysis ScreenTape or Bioanalyzer RNA pico kit (Agilent). 10ng of RNA was reverse transcribed using oligo-dT30 VN primer in the presence of Template Switching Oligo (TSO) with SuperScript II reverse transcriptase. cDNA was pre-amplified with IS PCR primers and PCR products were cleaned up with Ampure XP beads. One ng of PCR product was used to generate library using NexteraXT library prep kit (Illumina) and tagmentated DNA was amplified with indexed primers for a 12 cycles PCR followed by purification with AmpureXP beads. RNA-seq, 50bp single end with HiSeq2500
attributes.tag: GEO Accession
attributes.value: GSM3213954