title: GSM3672210: Day 50 NM organoids 1-3; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were dissociated mechanically using accutase solution and incubation at 37C. After single cell formation, the cell suspension was run through a 70μm strainer to remove any residual cell and debris clumps. A small volume of the cell suspension was removed for cell counts. The remaining cells were diluted 4-fold in 1X dPBS and then centrifuged at 200g for 5  minutes. The cell pellet was then resuspended in dPBS containing 0.04% BSA to give a final concentration of 206 cells/μl. PolyA selection based RNA capture (Chromium Single Cell 3' Reagent Kit , v2 Chemistry, 10X Genomics) was done as per the manufacturer's instructions provided by 10X Genomics platform.   RNA-Seq libraries were prepared as per manufacturer's instructions provided by 10X Genomics platform  (https://www.10xgenomics.com/single-cell/)
attributes.tag: GEO Accession
attributes.value: GSM3672210