title: GSM2550156: RNA140717RH_43; Mus musculus; RNA-Seq
library_construction_protocol: Frozen brains were slightly thawed and dissected by hand under RNAse-free conditions. Using the optic chiasm as the rostral marker, a 2 mm coronal slice of brain tissue was isolated. Beginning at the medial ventral aspect of the striatum and recognizing that the striatum has a partial cone shape, the dissection moved dorsal 1 mm, followed by a cut to the lateral boundary of the striatum, with a final cut following the lateral-ventral boundary. The isolated tissue was immediately placed into 1 ml of Trizol (Invitrogen, Carlsbad, CA). Library formation (polyA+, stranded) and sequencing were all performed according to Illumina’s specifications at the OHSU Massively Parallel Sequencing Shared Resource. Libraries were multiplexed 6 per lane, yielding approximately 25 to 30 million total reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM2550156