title: H3K36me3 ChIP-Seq analysis of H1 embryonic stem cell line (HS1347)
design: H3K36me3 ChIP-Seq analysis of H1 embryonic stem cell line (HS1347) using Illumina Genome Analyzer IIx
library_construction_protocol: Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: EXPERIMENT_TYPE
attributes.value: Histone H3K36me3
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
attributes.value: Bioruptor
attributes.tag: EXTRACTION_PROTOCOL_SONICATION_CYCLES
attributes.value: 25 min
attributes.tag: CHIP_PROTOCOL
attributes.value: Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: CHIP_PROTOCOL_CHROMATIN_AMOUNT
attributes.value: 35.3 ug
attributes.tag: SIZE_FRACTION
attributes.value: 80-280 bp
attributes.tag: CHIP_PROTOCOL_BEAD_TYPE
attributes.value: Protein G Mag Beads
attributes.tag: CHIP_PROTOCOL_BEAD_AMOUNT
attributes.value: 30 ul
attributes.tag: CHIP_PROTOCOL_ANTIBODY_AMOUNT
attributes.value: N/A
attributes.tag: CHIP_ANTIBODY
attributes.value: H3K36me3
attributes.tag: CHIP_ANTIBODY_PROVIDER
attributes.value: Abcam
attributes.tag: CHIP_ANTIBODY_CATALOG
attributes.value: ab9050
attributes.tag: CHIP_ANTIBODY_LOT
attributes.value: 395432