title: GSM3214051: Brain csf1ra-/-;b+/- #3; Danio rerio; RNA-Seq
library_construction_protocol: Zebrafish were sacrificed with ice water. Then brains were taken out of the skull and trypsinized on ice for 2 hours. Brains were flash frozen in liquid nitrogen. Total RNA extraction was performed using Trizol. cDNA libraries were created using reverse transcription, pre-aplification and Tn5 tagmentation. Illumina RNAseq libraries were prepared from cDNA using the Illumina TruSeq™ RNA Sample Prep Kit v2 according to the manufacturer's instructions (Illumina Inc.). In all libraries 50 nucleotide single-end reads (SR50) were sequenced on an Illumina HiSeq2500 sequencer according to the manufacturer's protocol, obtaining 10-20 million reads per sample library.
attributes.tag: GEO Accession
attributes.value: GSM3214051