title: GSM2981013: Smad23DKO_EpiLC_clone_KT15.1F6_differentiation1 (RNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from ~1.5 x10^6 Smad2/3 DKO and wild type EpiLCs from three independent EpiLC differentiations per genotype. These cell samples were taken from the same cells that were also used for ATAC-seq (see below). Total RNA was normalised to 800ng per sample, depleted of cytoplasmic and mitochondrial ribosomal RNA sequences (Ribo-Zero Gold rRNA Removal Kit (H/M/R), Cat: #MRZG12324) and used for library preparation Libraries were constructed using the Illumina TruSeq Stranded Total RNA Library Prep (H/M/R), Cat: #20020597) followed by sequencing (75-cycle paired end) on the Illumina HiSeq4000 platform.
attributes.tag: GEO Accession
attributes.value: GSM2981013