title: GSM3211519: Amhr2Cre/+, R26R YFP/+ male biological rep1; Mus musculus; RNA-Seq
library_construction_protocol: YFP+ Müllerian duct mesenchymal cells were directly sorted into TRI reagent RT-LS (Molecular Research Center Inc., Cincinnati, OH, USA) using a BD FACS Aria I cell sorter (BD Biosciences, USA) and homogenized. Total RNA was isolated following the manufacturer's recommended protocol with the following modifications; PelletPaint (EMD Millipore, Billerica, MA, USA) was added to samples to visualize RNA pellets and an additional ethanol precipitation was performed to remove any remaining phenol. cDNA libraries were generated using the TruSeq v2 kit (Illumina, San  Diego, CA,USA) following the manufacturer's recommended protocol from three biological replicates each of male and female. Each replicate consisted of ≥ 100 ng of total RNA isolated from pooled YFP+ Müllerian duct mesenchyme cells from 2-7 embryos. Individual library quality was assessed by high sensitivity DNA Bioanalyzer chip (Aligent, Santa Clara, CA) and the concentration of each library was determined using the Qubit picogreen assay (Life Technologies, Grand Island, NY). Equal quantities of each indexed library were pooled and sequenced on the Illumina HiSeq 2000 platform to generate paired end 76 base pair reads.
attributes.tag: GEO Accession
attributes.value: GSM3211519