title: GSM2267032: Liat-2 (Scr-MAP); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extrcted from tumors using the Qiacube (Qiagen) with the RNeasy kit (cat. no.  74106). Quality measurements of total RNA were performed using TapeStation (Agilent). RINe values were between 6.5-10.0 Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to the manufacturer protcol from 800 ng of total RNA.  mRNA pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). The six libraries were mixed into a single tube with equal molarity of all samples.  The RNAseq data was generated on NextSeq500 mid-output (75bp pair-end reads) kit v2 (Illumina, FC-404-2005).
attributes.tag: GEO Accession
attributes.value: GSM2267032