title: Illumina HiSeq 4000 paired end sequencing; Differential Regulation of Th17 and Th1 Cells in mouse by Glutaminase-Dependent Metabolism and Chromatin Remodeling
design: Differential Regulation of Th17 and Th1 Cells in mouse by Glutaminase-Dependent Metabolism and Chromatin Remodeling
library_construction_protocol: These CD4 cells are from WT mouse spleens, isolated for CD4+ T cells and differentiated in Th1 and Th17 skewing media over 5 days.For each of the TH-17 and Th-1 libraries, 50,000 live cells were harvested. Th1 and Th17 subsets were treated with vehicle or in the presence of 0.5 μM CB839 The ATAC-seq libraries were generated following a previously published protocol (Buenrostro et al., Nature Methods, 2013). The nuclei were isolated from the 50,000 cells.  The isolated nuclei were treated Tn5 Transposes from Nextera® DNA Sample Preparation Kit and the transposed DNA is purify using a Qiagen MinElute Kit. The transposed DNA libraries were amplify by PCR for 12 cycles and isolated by Ampure XP beads.
attributes.tag: Experimental Factor: compound
attributes.value: CB-839
attributes.tag: Experimental Factor: dose
attributes.value: 0.5
attributes.tag: Experimental Factor: cell type
attributes.value: T-helper 17 cell