title: GSM2458808: HT-29-MTX, A. ceylanicum treated #1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from the samples was purified using Trizol (Thermo Fisher).  The purification procedure deviated from the commercial Trizol protocol in the following regards: 1) after the first phase separation, an additional chloroform extraction step of the aqueous layer was performed using Phase-lock Gel tubes (5 prime), 2) 1 µL of GlycoBlue (Thermo Fisher) was added immediately prior to the isopropanol precipitation, and 3) the RNA was washed twice with 75% ethanol.  RNA sample quality was confirmed by spectrophotometry (NanoDrop) and with a fragment analyzer (AATI Fragment Analyzer).  For each sample, 1 µg of total RNA was used for further analysis.  PolyA+ RNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Directional RNA-seq libraries were prepared from 1 ug total RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.
attributes.tag: GEO Accession
attributes.value: GSM2458808