title: GSM2882422: ClonTechv4_Xins-cell-34; Schmidtea mediterranea; RNA-Seq
library_construction_protocol: For plate based single cell RNA-seq assay, cells were harvested into 96-well plate directly, and cDNA libraries were prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara) as per manufacturer's recommendations using 18 cycles of amplification.  Specifically, reactions were run at  the recommended volume.  Libraries were made using the Nextera XT kit (Illumina). After the PCR step, the libraries were individually cleaned with 0.8x AMPure XP SPRI beads (Beckman Coulter), and eluted in Resuspension Buffer. The libraries were quantified using Quant-IT DNA High-Sensitivity Assay Kit (Invitrogen) and examined using a high sensitivity DNA chip (Agilent). All libraries were then combined to one pool at equimolar concentrations for sequencing.  Finally, samples were sequenced deeply using a HiSeq 2500 sequencer. cDNA libraries were prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara) as per manufacturer's recommendations using 18 cycles of amplification.
attributes.tag: GEO Accession
attributes.value: GSM2882422