title: RNA-seq of mouse atria
design: Total RNA was isolated from left atria with the RNeasy Mini Kit (Qiagen). For RNA sequencing amplified cDNA was prepared from 10 ng total RNA according to the manufacturers protocol (Oviation RNA-Seq System V2, NuGen). After purification SPIA-cDNA was fragmented with 25 cycles (30 sec ON/ 90 sec OFF, low energy) using a Bioruptor (Diagenode). 100 ng of fragmented cDNA was used for library preparation (NEBNext UltraTM DNA Library Prep Kit, NEB). 9-12 PCR cycles were used for library enrichment. Agencourt AMPure XP Beads (Beckman Coulter) were used for purification.