title: Oobius  primorskyensis genomic sequences
design: Oobius primorskyensis from the Russian Far East (near Vladivostok) were reared using freshly laid eggs of A. planipennis on coffee filter paper at the BIIRU quarantine laboratories (USDA-ARS, Newark, Delaware) for 3 – 10 generations before DNA was extracted.  For each species, 30 – 50 adult parasitoids were killed in molecular grade ethanol, their DNA extracted with the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA), then quantified with the Quant-iT PicoGreen dsDNA Kit (Invitrogen, Grand Island, NY).  DNA from each species was used to make genomic libraries with the standard Illumina TruSeq DNA Sample Preparation Kit for paired-end libraries.  The libraries were sequenced as 150-nucleotide, paired-end, reads on an Illumina HiSeq 2500 at the Delaware Biotechnology Institute, Newark, Delaware, which produced 109 million reads that included 16 gigabases of DNA sequence for each species.