title: GSM1859049: msn2Δmsn4Δ-RNA-t7; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: 2 OD cycling cells were collected and flash frozen. RNA was extracted with the Qiagen RNeasy Mini kit (QIAGEN, 74104, Valencia, CA). RNA-seq libraries were prepared using Illumina Trueseq RNAseq v2 library kit (Illumina, San Diego, CA). PolyA beads were used for mRNA selection. 500 ng of RNA per sample was used as input and 12 cycles of PCR were run for amplification.
attributes.tag: GEO Accession
attributes.value: GSM1859049