title: GSM2109654: TM2 [miRNA]; Solanum tuberosum; miRNA-Seq
library_construction_protocol: Total RNA was isolated from leaves and tuber’s peel with TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s procedure. Equal amounts of each RNA samples were pooled at 12, 24 and 48 h time points and the quality were checked by Agilent 2100 bioanalyzer. Small RNAs (18-30 nt) were isolated from total RNA by polyacrylamide gel electrophoresis, and ligated to 3‘illumina adapter. The adapter-ligated sRNA were transcribed into cDNA by Super-script II Reverse Transcriptase (Invitrogen) to generate sRNA libraries. Small RNA libraries were subjected to deep sequencing with Illumina HiSeq 2000 at LC Sciences (Hangzhou, China).
attributes.tag: GEO Accession
attributes.value: GSM2109654