title: GSM1861611: MII oocyte_2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction from pooled oocytes and embryos was preformed using the Qiagen AllPrep kit according to manufacturers instructions. Two stranded libraries were prepared for each stage using the TotalScript RNA-Seq kit (Epicentre, Cat. num. TSRNA1296). This approach enabled low inputs (5ng of total RNA/reaction), and random hexamer priming facilitated transcript coverage balance. Each cDNA library was then split and amplified for 12 or 14 PCR cycles, resulting in four technical replicates per developmental stage.
attributes.tag: GEO Accession
attributes.value: GSM1861611