title: GSM4659094: 20140606.B-16DAF_MM17_OVT; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: The RNA extraction was done with the Qiagen RNeasy Micro Kit following manufacturer's instruction manual. All RNA samples were adjusted to a concentration of 100 pg/µl prior to pre-amplification. Pre-amplification for the Illumina sequencing was done using the Clonetech SMARTer Ultra Low kit following the user's manual with small modifications. In brief, the RNA samples were subjected to first strand cDNA synthesis, followed by 15 cycles of Long Distance (LD) PCR. The PCR amplified cDNA was subsequently purified by immobilizaiton on AMPure XP beads for 15 min at RT, resuspended in 15 µl nuclease-free water and stored at -20°C for further experiments and library preparation. The TruSeq SR Cluster Kit v4-cBot-HS (Illumina, Inc, California, USA) was used for cluster generation using 8 pM of pooled normalized libraries on the cBOT.  Sequencing were performed on the Illumina HiSeq 2500 single end 126 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
attributes.tag: GEO Accession
attributes.value: GSM4659094