title: GSM2210597: R_WGBS_EpiG_IMR90_100_12_I12; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from the cell lines using standard phenol:chloroform extraction followed by ethanol precipitation. Whole Genome Bisulfite Sequencing libraries were prepared as:  100 ng of DNA were bisulfite converted using EZ DNA Methylation-Gold Kit and the single stranded DNA obtained processed for library construction using the EpiGnome Methyl-Seq kit as per manufacturer protocol. The DNA was made double stranded by the use of 5’tagged random hexamers and subsequently 3’ tagged with terminal tagging oligo. The di-tagged DNA was enriched using 10 cycles of PCR, with PCR indexed-primers compatible with Illumina sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2210597