title: AccuNGS_alt_lib_purification(gel)
design: pLAI.2 plasmid grown in DH5a and purified; Integrase PCR amplicon, 40 PCR rounds with Superfi Platinum polymerase; PCR product extraction from agarose gel; Nextera XT library preparation kit with 12 PCR rounds using Superfi Platinum polymerase; library purification using agarose gel; paired-end sequencing with Illumina MiSeq