title: GSM1667215: WT-IM-RNAseq-replication1; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: Rice SAM and IM total RNA samples were isolated using TRIzol reagent (Invitrogen).SAMs and IM (about 2 mm) were crosslinked in 1% formaldehyde under vacuum. Chromatin was extracted and fragmented to 200-750 bp by sonication, and ChIP was performed using the following antibodies: H3K27me3 (a2363, AbclonalABclonal ) and H3K4me3 (07-473, Millipore).The eluted ChIP DNA was used to generate Illumina sequencing libraries following the manufacturer's protocol. RNA libraries were prepared according to the protocol strictly (http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf).  Briefly, 1-4 μg of total RNAs were used for mRNA purification and cDNA synthesis. After single ‘A’ nucleotide was added to the 3’ ends of the blunt fragments of the double strand cDNAs, indexing adapters are ligated to the ends of the cDNA. PCR are performed to amplify the DNA fragments. The amplified DNA fragments were purified and sequenced with the Illumina Hiseq-2000 system.The eluted ChIP DNA was used to generate Illumina sequencing libraries following the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM1667215