title: GSM2310023: m293T; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from the cells [monolayer (mMCF-7 and m293T) and sphere (sMCF-7 and s293T) culture conditions] using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA). RNA was treated with RNase-free DNase I (Promega, Madison, WI, USA) to eliminate DNA contamination. The RNA concentration and quality were determined using a NanoDrop® ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA) and the Agilent RNA Integrity number (RIN) (Schroeder et al., 2006) was evaluated using the Agilent 2100 Bioanalyzer and the Agilent RNA 6000 Nano kit (Agilent Technology, Santa Clara, CA, USA). The absorbance ratio at 260:280 nm for all samples was >1.8 and the RIN value was > 9. The integrity of RNA samples was also ascertained by the presence of distinct 28S and 18S ribosomal RNA bands in agarose gels after electrophoretic resolution. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM2310023