title: RNA-Seq of male Tytthus chinensis
design: Total RNA was extracted using Trizol reagent. Poly (A)+ RNA was purified from 20  g total RNA using oligo (dT) magnetic beads and fragmented into short fragments in fragmentation buffer randomly. The first-strand cDNA was synthesized using random hexamer. Second-strand cDNA was generated by adding buffer, dNTPs, RNaseH, and DNA polymerase I