title: GSM2309041: HCT116WT-untreated BS-seq; Homo sapiens; OTHER
library_construction_protocol: The construction of methyl-seq library was carried out according to the protocol provided by Agilant. 1ug of genomic DNA is shared to around 125 ~ 175bp using a Covaris sonicator. The sheared DNA was end-repaired and ligated with methylated adapters. 500ng of the library DNA was hybridized with biotinylated oligo probes to capture single strand target DNA on the magnetic beads. The captured DNA was converted by bisulfite treatement and purified. The library was firstly amplified by 8 cycles of PCR. After purification, this PCR product was further amplified by 6 cycles of PCR and sequenced using illumina Hi-seq2000.
attributes.tag: GEO Accession
attributes.value: GSM2309041