title: 16S rDNA of bacterial community in anerobic bioremediation of tannery chromium wastes
design: Libraries of bacterial 16S rRNA gene fragments were amplified from each DNA extract using PCR with universal bacterial primers 27F and 1492R, followed by a second nested PCR using 27F and 518R primer pairs having overhang adapter sequences compatible with Illumina indexes and sequencing adapters . Resultant PCR products were cleaned, indexed the library, before pooling and sequencing on Illumina Miseq 250 to generate paired