title: Illumina HiSeq 2000 sequencing; miRNASeq of musculoskeletal ageing using human mesenchymal stem cells and their tissue constructs
design: miRNASeq of musculoskeletal ageing using human mesenchymal stem cells and their tissue constructs
library_construction_protocol: MSCs purchased from young and old donors grown in osteocenic media for 21 days grown in pellet culture in chondrogenic media miRNAeasy columns for RNA purification following Trizol treatment New England BioLabs (NEB) NEBNext Multiplex Small RNA Library Prep Set for Illumina, according to manufacturer’s instructions
attributes.tag: Experimental Factor: age
attributes.value: old
attributes.tag: Experimental Factor: clinical information
attributes.value: tendongenic
attributes.tag: LIBRARY_STRAND
attributes.value: second strand