title: genome sequencing of an isolated salmonella Typhimurium resistant strain  to an antimicrobial peptide
design: DNA-Seq was performed as previously described (Blecher-Gonen et al., 2013) with the following modifications: 300-600ng of gDNA was sheared using the Covaris E220X sonicator (Covaris,Inc.,Woburn, MA, USA). End repair was perfomed in 80ul reaction at 20C for 30min. Following Agencourt AmPURE XP beads cleanup (Beckman Coulter, Inc., Indianapolis, IN, USA) in a ratio of 0.75X Beads/DNA volume, A bases were added to both 3 ends followed by adapter ligation in a final concentration of 0.125uM. A  SPRI bead cleanup in a ratio of 0.75x beads/DNA volume was performed, followed by 8 PCR cycles using 2X KAPA HiFi ready mix (Kappa Biosystems, Inc. Wilmington, MA, USA) in a total volume of 25ul with the following program: 2min at 98C, 8 cycles of 20sec at 98C, 30sec at 55C, 60sec at 72C following by 72C at 10min.