title: In vitro simulation of the pig cecum to study subtherapeutic concentrations of doxycycline
design: an in vitro simulation of the pig cecum was used to study the effects of three concentrations of doxycycline: 1, 4 or 16 mg/L DOX. The chemostat was used for 10 days, on day 5, one of the three concentrations of DOX was supplemented to the chemostat through the feed medium. This sample is run 4, day 8 of chemostat; 4 mg/L DOX