title: Complete Genome of  FMDV O/ME-SA/Ind2001BD2 sublineage strain
design: The virus was isolated from BHK-21 cell line, and viral RNA was extracted from infected cell culture supernatant of passage 3 in an automated Maxwell 16 system using the Maxwell 16 total RNA purification kit (Promega, USA) followed by cDNA synthesis using the GoScript Reverse Transcription System with oligo(dT) and random primers. Afterwards, seventeen overlapping FMDV-specific primer pairs based on the BAN/NA/HA-156/2013 sequence were used to amplify the near-complete genome. Sequencing reactions consisted of 2 ul of BigDye Terminator v. 3.1 mix (Applied Biosystems), 3 ul of dilution buffer (Applied Biosystems), 5-20 pmol of primer, and 0.2 ug of template DNA in a final reaction volume of 20 ul.  Cycle conditions were an initial denaturation at 96 for 2, then 35 cycles of 96 for 10, 52 for 15, 60 for 3', followed by 1 at 72.  Excess dye terminators were removed using CleanSeq magnetic bead sequencing reaction clean up kit from Beckman Coulter(Agencourt Bioscience).  The samples were resuspended off of the beads in 50 ul of ddH2O.  10 ul of each sample was loaded into a 96 well PCR plate, an additional 10 ul of ddH2O was added to each well, and the plate loaded onto the sequencers according to the manufacturer's instructions.