title: EdUseq-HU of U2OS cells overexpressing cyclin E for 2-3 days: 6 h after mitotic shake-off not treated with DRB (control)
design: U2OS cells overexpressing cyclin E were synchronized by mitotic shake-off and released into media containing HU and EdU for 6 h before being collected. No DRB was added (control). The EdU labeled DNA was then isolated and sequenced.