title: GSM2310022: KO3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the colorectal cancer tissues using the RNAeasy Mini Kit (Qiagen, Valencia, CA). Total RNA prepared was submitted to Shanghai Personal Biotechnology Co., Ltd, where RNA intergrity was confirmed using the Illumina HiSeq 2000 system. mRNA was purified from total RNA by oligo-dT magnetic beads and normalized to 150 bp short fragments. Double-strand cDNA libraries were prepared and constructed using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA). The synthesized cDNA libraries were diluted into 4-5 nM and sequenced.
attributes.tag: GEO Accession
attributes.value: GSM2310022