title: GSM4483497: CD34; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from cells dissolved in Trizol (Invitrogen) according to manufacturers protocol except for using two chloroform extractions instead of one. After isopropanol precipitation RNA pellets were dissolved in nuclease free water (Ambion) Libraries were prepared for sequencing using the standard protocol QuantSeq 3'mRNA-SEQ Library Prep Kit (Lexogen). Input was 1,000 ng RNA and 11 PCR cycles were applied.
attributes.tag: GEO Accession
attributes.value: GSM4483497