title: WGS of  Lactobacillus murinus, strain NM26_J9: murine gut isolate
design: Sequencing  performed by Dr. Brian Yu at the Genomics Platform of the Chan Zuckerberg  Biohub. Nextera XT kits were used to  make the libraries with CZ Biohubs custom designed adapters - 12 bp index  sequences on each side of the library and are dual unique so that each  sequence only appears once. The libraries were made in 384 well plates in 4  uL of total volume. Genomic DNA was diluted to 0.18 ng/uL and 0.4 uL was  added to the library prep reaction using Mosquito HTS from TTP. 0.5 uL of the  library was pooled for sequencing. Sequencing was performed on a NovaSeq S4  flow cell with the run configuration 140 (12) (12) 140, so thats 2x140 bps  with dual 12 bp index sequences.