title: SMRT HiFi WGS of human: HEK293 DNA
design: A 20 kb HiFi  sequencing library was prepared using the SMRTbell Express Template Kit 2.0  (Pacific Biosciences) according to manufacturer's instructions. The SMRTbell  library was sequenced on two 8 M SMRT cells on the Sequel II system using 2.0  sequencing and binding chemistry, 2 hrs pre-extension time and 30 hrs movie  time.