title: GSM3677684: ZH11-N3-RNAseq; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: The total RNAs were extracted from the leaves of SNAC1-OE lines and ZH11-WT plants using TRIzol reagent (InvitrogenTM) according to the manufacturer's instructions. The ChIP assay was performed according to Chris Bowler (Bowler et al., 2004) with some modiﬁcations. Briefly, 3g shoot tissues from 4-leaf-stage seedlings were cross-linked in 1% formaldehyde by vacuum for 30 minutes. The chromatin was extracted on ice as described by Bowler method and sheared to 200-500 bp fragments by sonication. Subsequently, the DNA fragments were immunoprecipitated by custom-made anti-SNAC1 polyclonal antibody generated using full-length SNAC1 protein. The combined DNAs were eluted, purified, and dissolved in ddH2O. RNA-seq and ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM3677684