title: GSM2706361: WT 1C No treatment; Staphylococcus aureus USA300-0114; RNA-Seq
library_construction_protocol: RNA was isolated from the bacteria using RNAeasy Mini Kit (Qiagen). Quality control of RNA samples was done to determine RNA quantity and quality prior to their processing by RNA-seq. The concentration of RNA samples was determined using NanoDrop 8000 (Thermo Scientific) and the integrity of RNA was determined by 2100 Bioanalyzer (Agilent Technologies). Ribosomal RNA depletion was performed using a Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, San Diego, CA) according to the manufacturer’s instructions. RNA-seq library generation was continued using the TruSeq RNA protocol (Illumina) starting at the “Elute, Prime, Fragment” step and was completed according to the manufacturer’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2706361