title: EdUseq-HU/release of U2OS cells overexpressing cyclin E for 2-3 days: 10 h HU arrest and release for 150 min.
design: U2OS cells overexpressing cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 10 h, cells were then released from HU for 150 minutes with EdU being added 30 minutes before collection. The EdU labeled DNA was then isolated and sequenced.