title: GSM2884396: DNA methylation in hormone-free T47D breast cancer cells; Homo sapiens; MeDIP-Seq
library_construction_protocol: Hormone-deprived T47D cell pellet has been resuspended in Tris-EDTA and 20 μg of proteinase K was added. The mix has been then incubated at 55°C for 16 hours and DNA was extracted using first 1 volume of phenol and the 1 volume of chloroform. The DNA was precipitated with ethanol and sodium acetate and then resuspended in Tris-EDTA containing 20 μg/ml RNAse A The library has been prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (E7370L) following manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2884396