title: GSM2807179: BMd; Malus domestica; miRNA-Seq
library_construction_protocol: According to the instruction of TrizolTM Reagent (Invitrogen, Carlsbad, CA), total RNA was extracted from each sample, and RNA purity was evaluated using NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA Nano 6000 Assay Kit and Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) were used to assess RNA concentration and integrity. Three repeats of control samples and inoculation samples were mixed for small RNA library construction, respectively. Three μg of total RNA per sample was used as input material for the small RNA library. Small RNA library construction was proceeded following the detailed protocol provided by the genome sequencing company (Novogene, China). The detailed protocol of miRNA-seq was provided by the genome sequencing company (Novogene, China) and the protocol of degradome sequnce was followed with  the published parallel analysis of RNA Ends (PARE) protocol (German et al., 2009)..
attributes.tag: GEO Accession
attributes.value: GSM2807179