title: 16S amplicon sequencing of seawater affected by jellyfish decomposition replicate 2
design: The V4 region of the 16S rRNA gene was amplified using the modified primer pair 515F-806R (Apprill et al. 2015; Parada et al. 2016) in combination with CS1/CS2 tags (CS1_515Fc 5-ACACTGACGACATGGTTCTACA GTGYCAGCMGCCGCGGTAA, CS2_806Rc 5-TACGGTAGCAGAGACTTGGTCT GGACTACNVGGGTWTCTAAT), using the following PCR amplification protocol: initial denaturation at 94 C for 45 s, 30 cycles of denaturation (94 C for15 sec), annealing (15 cycles at 50 C and 15 cycles at 60 C for 20 sec) and extension (72 C for 30 s).Library preparation from the PCR products and sequencing of 2x250 bp Illumina MiSeq reads was performed at HyLabs (Israel).