title: Rht-D1 BAC clone
design: Rht-D1 BAC plasmid DNA (> 5g) was prepared using a ZR BAC DNA Miniprep Kit. Plasmid quantity and quality were determined on a Nanodrop 2000 spectrophotometer . DNA samples were processed for a paired-end (PE) DNA library and sequenced  in pools on a Miseq platform