title: GSM2310339: gurken_tagmentation_UT; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol, and treated with DNase. rRNA was depleted using RNaseH, and tRNA species were depleted using size exclusion columns. A gene-specific primer was used for reverse transcription with the TGIRT enzyme. Gene-specific PCR primers were used to amplify the region of interest, and then dsDNA was fragmented and prepared for sequencing using NexteraXT (Illumina). Gene specific RT/PCR followed by Nextera XT or LMPCR. For genome-wide libraries we used 3' ligation, followed by circularixation, and PCR to add the sequencing adapters.
attributes.tag: GEO Accession
attributes.value: GSM2310339