title: GSM3593778: H3K27ac chipseq in NSCs; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin lysate was extracted from fix cells and DNA was sonicated to about 200 bp in length using Bioruptor (Diagenode). Chromatin lysate from one million cells were incubated with 2 ug of TY1 antibody (Diagenode), and precipitated DNA was eluted from Protein A/G beads using PCR purification kit (Qiagen). DNA libraries generated from ChIP-DNA and input-DNA were deep-sequenced using HiSeq pair-end 50 bp.
attributes.tag: GEO Accession
attributes.value: GSM3593778