title: GSM3732685: siE1A_4d_mRNAseq_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: 1X10^6 HEK293 were transfected with indicated siRNAs for 4, 8, or 16 days. RNA was isolated using QIAGEN RNeasy Plus Mini Kit. Eluted RNA was treated with Ambion DNA-free™ DNA Removal Kit and then Ambion TRIzol reagent, precipitated with isopropanol, and dissolved in sterile water. RNA concentration was measured with a Qubit fluorometer. One microgram of RNA was fragmented and copied into DNA then PCR amplified with bar-coded primers for separate samples to prepare sequencing libraries using the Illumina TruSeq RNA Sample Preparation procedure.
attributes.tag: GEO Accession
attributes.value: GSM3732685