title: GSM3223411: S320: DARP-EGFP line 1, scrambled siRNAs, replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: 72 hours post transfection, total RNA was extracted with the RNEasy (Qiagen) kit from 750,000 cells per sample.  Either ERCC mix1 or mix2 external standards (Invitrogen) were diluted 1:100 in water and 1 ul was added to each lysate. Sequencing libraries were generated by SeqMatic (Fremont, CA). rRNA was depleted with the Ribo-Zero Gold rRNA Removal Kit (Illumina) and sequencing libraries were prepared with the TruSeq Stranded Total RNA kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3223411