title: GSM2210594: IMR90-_2; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from the cell lines using standard phenol:chloroform extraction followed by ethanol precipitation. Agilent SureSelect Methyl-Seq libraries were made according to the company’s specifications using 3ug and 1ug of DNA. DNA was sonicated using a Covaris S220 sonicator to obtain products of 150-200bp. DNA was then end-repaired, A-tailed and ligated with methylated adapters to create pre-capture DNA libraries. DNA libraries were then hybridized to the RNA SureSelect Human methyl-seq capture library at 65°C for 16 hrs. Hybridized products were purified by capture with Strepdavin beads and then subjected to bisulfite conversion (64°C for 2.5hr) using the Zymo EZ DNA Gold kit. The bisulfite treated libraries were PCR amplified for 8 cycles with Agilent Taq to get the required amount of DNA library and then indexed by another 6 cycles of PCR amplification to create the final libraries.
attributes.tag: GEO Accession
attributes.value: GSM2210594