title: GSM2310391: Fibroblasts infected Human and Candida with T cells BR3; Homo sapiens; Candida albicans SC5314; RNA-Seq
library_construction_protocol: Tissue cell layers were separated, quickly washed in PBS and immediately frozen down in liquid nitrogen. Disruption was carried out using a Mixer Mill MM 200 (RETSCH) with a shaking frequency of 30/s. The resulting powder was resuspended in lysis buffer RLT (QIAGEN, Hilden, Germany) supplemented with 0.01% v/v of ß-mercaptoethanol. The extraction of total RNA was performed according to QIAGEN's Protocol for the isolation of total RNA using the RNeasy Plus Mini Kit. Concentration and integrity of total RNA was analyzed using the Agilent 2100 Bioanalyzer using the RNA Nano kit. TruSeq RNA Sample Preparation Kit v2 (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM2310391