title: 45-3-1 (rep2)-20160313
design: The small RNA library was constructed using Illumina TruSeq small RNA sample prep kit. 18-30 nt small RNA was purified form 10 ug total RNA using Urea PAGE. The size selected RNA was ligated with 5・ and 3・ adapter. The adapter-ligated small RNA was treated with reverse transcription and amplified with 15 cycles. Amplified cDNA was size select and purified using PAGE to remove the adapter dimer. After validating the libraries by QPCR, Expersion and Qubit, the library was sequenced using Illumin HiSeq 2500.