title: GSM4263996: mreB genomic mutation library; Escherichia coli; OTHER
library_construction_protocol: Fifty µL of the scraped cell suspension was subjected to centrifugation in a 1.5 mL tube. The cells were washed twice with PBS and as much of the supernatant was removed as possible. Finally, the cells were suspended in 50 µL TE buffer (pH 8.0). The resuspended cells were boiled at 100 OC for 10 minutes. The genomic region to be sequenced was amplified using primers with Illumina Nextera adapters
attributes.tag: GEO Accession
attributes.value: GSM4263996