title: Sponge-associated microbial communities in Haliclona fulva
design: 16s rRNA gene amplicons comprising the hypervariable region V4 were amplified using primers 515f and 806r, which have previously been used to successfully target, retrieve, quantify and classify simultaneously Eubacterial and Archaeal reads. Libraries were constructed using paired-end technology and all samples were sequenced using the Illumina-MiSeq platform (Sequencing Facilities, Alkek Center for Metagenomics and Microbiome Research, Houston-USA)