title: Oxford nanopore long reads for Draft(library 2, March 2018) for hybrid long-read assembly of Candida metapsilosis ATCC 96143
design: 1 g of genomic DNA was sheared in a gTube (Covaris, Woburn, MA) for 1 min at 6,000 rpm in a MiniSpin plus microcentrifuge (Eppendorf, Hauppauge, NY). The sheared DNA was converted into a shotgun library with the LSK-108 kit from Oxford Nanopore, following their manual. The library was sequenced on a SpotON R9.4 RevC flowcell for 48 h using a MinION MK 1B sequencer.  Base calling was performed from the Oxford Nanopore FAST5 data using Albacore v2.3.1 (Oxford Nanopore) to generate FASTQ reads used in the assembly along with a sequencing summary file.