title: GSM3466582: CTR Sample5; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were homogenized and total RNA was isolated by Trizol® reagent (Life Science Technologies, Italy) following the manufacturer's specifications. RNAs were quantified using a Nanodrop ND-100 Spectrophotometer (Nanodrop Technologies, Wilmington, USA) and a 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit, Waldbronn, Germany); RNAs with a 260:280 ratio of ≥1.5 and an RNA integrity number of ≥8 were subjected to deep sequencing. Sequencing libraries were prepared by the Illumina TruSeq Targeted RNA Expression NF-kB Panel integrated with BACE1, VDR and MGAT3 genes, using XX-ng total RNA (Illumina). The qualities of the libraries were assessed by 2100 Bioanalyzer with a DNA1000 assay. Libraries were quantified by qPCR using the KAPA Library Quantification kit for Illumina sequencing platforms (KAPA Biosystems); RNA processing has been carried out using Illumina MiSeq Sequencing.  
attributes.tag: GEO Accession
attributes.value: GSM3466582