title: Illumina HiSeq 2000 sequencing; miRNASeq of musculoskeletal ageing using human mesenchymal stem cells and their tissue constructs
design: miRNASeq of musculoskeletal ageing using human mesenchymal stem cells and their tissue constructs
library_construction_protocol: MSCs purchased from young and old donors grown in pellet culture in chondrogenic media grown as 3D tendonconstructs in tenogenic media miRNAeasy columns for RNA purification following Trizol treatment New England BioLabs (NEB) NEBNext Multiplex Small RNA Library Prep Set for Illumina, according to manufacturer’s instructions
attributes.tag: Experimental Factor: age
attributes.value: old
attributes.tag: Experimental Factor: clinical information
attributes.value: osteogenic
attributes.tag: LIBRARY_STRAND
attributes.value: second strand