title: Small RNA sequencing of AC7643 . Not stressed . Replicate II .
design: SmRNA libraries were constructed from root total RNA using commercially available reagent sets, following the manufacturer’s protocol (Solexa) and Wang et al. (5). Briefly, 16~35 nt smRNAs were separated from total RNA by 15% denaturing PAGE, and ligated to adaptors both at 5' and 3'. Ligation products were gel-purified by 10% denaturing PAGE, and reverse-transcribed. libraries were sequenced on HiSeq 2500 SE50 system, respectively. (Illumina, California, USA).