title: RNA-Seq of pear fruit:after 12h of mock treatment
design: The total RNA with high quality from each sample  were used for Illumina sequencing at Biomarker technologies (Beijing, China) according to the standard Illumina sample preparation protocol. Briefly, the poly (A) mRNA was enriched via magnetic oligo (dT) beads and then broken into short fragments using an RNA Fragmentation Kit (Beckman Coulter, USA). These cleaved mRNA fragments were used as templates for first-strand cDNA synthesis using random hexamer primers. Then, second-strand cDNA was synthesized and purified using AMPure XP beads. These short fragments were ligated to sequencing adapters, and the desired fragments were separated using AMPure XP beads. Next, the purified cDNA fragments were enriched via PCR. Finally, the six cDNA libraries were sequenced using Illumina HiSeq2000