title: GSM1666301: testis_E18 (rna-seq); Mus musculus; RNA-Seq
library_construction_protocol: Tissue total RNA samples for testis were purchased from Zyagen. Total RNA of H1 cells was isolated using Trizol (Life Technologies). Additional column purification and DNaseI treatment were applied using Direct-zol RNA kit (Zymo Research). rRNA was depleted using RiboMinus Trnascriptome ioslatoin kit (Life Technologies) from 10µg total RNA. ERCC Spike-in (Life Technologies) was added to 500 ng of rRNA depleted RNA. mRNA was isolated using the NEBNext Poly(A) mRNA magnetic isolation module. mRNA sequencing libraries were generated using the NEBNext Ultra directional RNA library Prep kit and NEBNext multiplex oligos for Illumina according to the manufacturer's instruction (NEB). The final libraries' concentrations were measured using the Qubit fluorometric assay (Life Technologies) and Bioanalyzer (Agilent) for the quality check.
attributes.tag: GEO Accession
attributes.value: GSM1666301