title: GSM3526636: Non-microglia cells from Trem2 +/- animal 8042 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq
library_construction_protocol: Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer.
attributes.tag: GEO Accession
attributes.value: GSM3526636