title: Deciphering the differential gene expression apex  cochlea sensory epithelias
design: Two cDNA libraries of the apical sensory epithelia samples and basal samples were constructed according to the Illumina manufacturer’s instructions. the library was pair-end sequenced from using Illumina NestSeq 500. Raw image data generated by sequencing was transformed by base calling into sequence data, which is called raw data/raw reads, and was stored in fastq format. Raw sequencing data were filtered to remove low quality reads before subsequent analysis. All assembled common genes were compared the average expression levels, the genes showing at least a 2-fold difference (p<0.05) between apex and base were considered to be differentially expressed.