title: 16S rDNA sequencing of  soil  microbial structure
design: The universal primers F341 and R806 were chosen. Then at the 5 'end of the universal primer, the index sequence and linker sequence (forward primer  5'-3'  ACTCCTACGGGRSGCAGCAG  F341 ; reverse primer 5'-3'  GGACTACVVGGGTATCTAATC  R806  were added to be suitable for sequencing. Using the diluted genomic DNA as a template to PCR amplification with the high-fidelity enzyme KAPA HiFi Hotstart ReadyMix PCR kit ensures the accuracy and efficiency. PCR products were monitored on 2% agarose gel and samples with bright main strip about 425 bp were chosen for further analysis. The sequencing of PCR products was performed on an Illumina HiSeq2500 platform and 250 bp paired-end reads were generated. The raw data were then subjected to a quality control procedure.