title: RNA-Seq of Coniochaeta pulveracea: Acacia
design: mRNA was captured from 5ug total RNA using the Dynabeads mRNA DIRECT Micro Kit. The Ion Total RNA-Seq Kit v2 was used to convert expressed mRNA transcripts into a representative cDNA library for strand specific RNA sequencing on the Ion Torrent Proton system. The libraries were diluted to a target concentration of 80pM and pooled in equimolar amounts for sequencing template preparation using the Ion PI HiQ Chef Kit. Twenty five microliters of diluted, pooled barcoded library was loaded onto the Ion Chef liquid handler for template preparation and enrichment using Ion PI HiQ Chef. Enriched ion sphere particles were loaded onto an Ion PI v3 Chip.