title: GSM2730296: control mouse brain, total RNA, late symptomatic, rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Cytoplasmic lysates from frozen mouse tissues and polysomal profiling were performed as described previously (Lunelli et al., 2016).RNA was extracted from control and SMA brains (at early and late symptomatic stage). Sucrose fractions from all fractions (total RNA) or polysomal fractions only (polysomal RNA) were pooled. The fractions were first digested with proteinase K (100 mg/mL, Euroclone) for 90 minutes at 37°C and then RNA was extracted with acid-phenol:chloroform:isoamylalcohol, pH 4.5 (Sigma Aldrich) and isopropanol precipitation. RNA libraries were prepared for sequencing using standard Illumina protocols, using the TruSeq® Stranded Total RNA (Illumina) kit
attributes.tag: GEO Accession
attributes.value: GSM2730296