title: NRCM from Rattus norvegicus Control_PBS 48h
design: Total RNA was isolated from NRCMs with the RNeasy Mini Kit (Qiagen). Starting material for library preparation was 500 ng total RNA. mRNA was isolated using magnetic beads (NEBNext® Poly(A) mRNA Magnetic Isolation Module, NEB). Library preparation was performed with NEBNext® UltraTM RNA Library Prep Kit for Illumina®. 13 PCR cycles were used for library enrichment. Agencourt® AMPure® XP Beads (Beckman Coulter) were used for purification and size selection.