title: GSM1665987: Mel-RNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Mini Kit (Qiagen) Sequencing libraries were prepared following the Illumina TruSeq Stranded Total RNA library prep protocol, with Ribo-Zero (RS-122-2201 or -2202), starting from 300-500ng of DNase I-treated total RNA, with the exception of 13 cycles of PCR amplification instead of 15. Amplified libraries were purified using AMPure beads, quantified by Qubit and qPCR, and visualized on an Agilent Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM1665987