title: WGS of Deuterocohnia longipetala: leaf material
design: A 5  g DNA aliquot was fragmented by nebulization, and barcoded libraries were prepared as outlined in the Rapid Library Preparation Method Manual supplied by Roche Diagnostics (Mannheim, Germany). The libraries were quantified by fluorometry (TBS-380, Turner Biosystems, Sunnyvale, California, USA) and shotgun-sequenced on a Roche 454 GS-FLX with the Titanium Sequencing Kit XLR70 and the Titanium PicoTiterPlate Kit (Roche Diagnostics), following the manufacturer   s protocols