title: GSM2551925: ChIPseq, H3K27ac Ebf2KO; Mus musculus; ChIP-Seq
library_construction_protocol: Interscapular BAT was isolated as described in Harms et al., Genes Dev., 2015. The tissues were minced and crosslinked in 1 % formaldehyde/PBS. To prepare ChIP extracts, crosslinked tissue was suspended in a ChIP buffer containing 20mM Tris, 0.1% SDS, 1% Triton-100, 150 mM NaCl, 1 mM EDTA, and protease inhibitors. Samples were sonicated under conditions optimized for IP efficiency. Extracts were then cleared of insoluble lipid by microfuge centrifugation at full speed followed by transfer to a new tube. IP was performed with indicated antibodies and genomic DNA purified. ChIP-Seq libraries were produced and sequenced according to Illumina protocols. Libraries were multiplexed for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2551925