title: UV mutant 2
design: Genomic DNA was extracted according to the method of (Hoffman and Winston, 1987) and purified by an additional purification step using the MasterPure Complete DNA and RNA Purification Kit (Epicentre). DNA concentration was determined by PicoGreen measurements (Thermo Fisher Scientific). 1 µg genomic DNA was subsequently sheared in micro AFA tubes using an S220 focused ultra-sonicator with AFA technology (Covaris). Average fragmentation size was assessed by performing a Fragment Analyzer run with the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies). Fragmented DNA was subsequently converted into indexed libraries for Next Generation Sequencing using the NEBNext®Ultra™ DNA Library Prep Kit for Illumina®. The library was prepared according to the manufacturer’s protocol.