title: GSM2309148: 18M+Nic-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃. The complementary DNA (cDNA) libraries for single-end sequencing were prepared using TruseqTM Small RNA sample prep Kit (Illumina) according to the manufacturer’s instructions. 1μg small RNA was used to add 3’ adaptor and 5’ adaptor to small RNA and RT-PCR was applied to the small RNA with adaptor to achieve the 1st cDNA; Truseq (Illumina) primer was used for PCR enrichment and introducing index sequence. Size selection was applied to obtain the 145-160bp library sequence. TBS380(Picogreen) was introduced to quantify the library concentration. We applied the cluster formation on flow cell utilizing cBot and sequencing on Hiseq2000 Platform.
attributes.tag: GEO Accession
attributes.value: GSM2309148