title: RNA-seq of Exophiala spinifera: adult skin
design: For RNA-seq analysis, raw reads were assessed for quality by FASTQC v0.11.5 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) and filtered to remove low-quality reads with Trimmomatic v0.36. Filtered reads were then aligned against to the corresponding reference genome of E. spnifera using STAR v2.5.3a. Read counts for each gene were calculated using FeatureCounts v1.5.2. Differentially expressed genes were detected by R Bioconductor package DESeq2 with the Benjamini-Hochberg adjusted p-value < 0.05 and fold change > 3.