title: RNA-seq of Oryza sativa; Pokkali Low  Nitrogen treatment R1
design: Oryza sativa  cultivar 'Pokkali' was grown in a growth chamber under nitrogen stress. Total  RNA was extracted using RNApro Solution (MP Biomedicals, California, USA)  based on manufacturer's protocol. DNAse treatment was done using PerfeCTa  DNase I (Quantabio, Massachusetts, USA) following manufacturers instructions.  mRNA was enriched using oligo(dT) beads.