title: GSM4271122: clone 2; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq was performed as previously described with minor modifications (Buenrostro et al., 2013). Fifty thousand cells were pelleted at 550 x g and washed with 1 mL 1x PBS, followed by treatment with 50 mL lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). After pelleting nuclei, the pellets were resuspended in 50 mL transposition reaction with 2.5 mL Tn5 transposase (FC-121-1030; Illumina) to tag and fragment accessible chromatin. The reaction was incubated in a 37C water bath for 45 minutes. Tagmented DNA was purified using a MinElute Reaction Cleanup Kit (QIAGEN) and amplified with 12 cycles of PCR. Libraries were purified using a QIAQuick PCR Purification Kit (QIAGEN). Libraries were paired-end sequenced (38 bp+37 bp) on a NextSeq 550 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4271122