title: GSM4314657: Input_KO; Mus musculus; RIP-Seq
library_construction_protocol: m6A IP was carried out using Magna MeRIP m6A Kit (Millipore) according to the manufacturer's instructions. total RNA was extracted using Trizol, DNaseI-treated and RNA was chemically fragmented into 100-nucleotide-long fragments and 350µg of total RNA were subjected to each immunoprecipitation (IP) with affinity purified anti-m6A antibody. RNA was purified using RNeazy Kit (Qiagen) and processed for library generation using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa). Sequencing was performed in 100pb paired-end reads using using Novaseq 6000.
attributes.tag: GEO Accession
attributes.value: GSM4314657