title: GSM4263538: hen2 30 min replicate 3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: A pool of 10 intact whole seedlings was flash frozen and total RNA was extracted using TRI-Reagent. Polysaccharide precipitation was carried out using the double sodium acetate method. CAGE libraries were prepared as in (Takahashi et al., 2012) with a starting material of 5ug of total RNA. Phi-X spike-ins were added (30%) to each sequencing lane to account for low complexity of the 5' ends of the CAGE libraries
attributes.tag: GEO Accession
attributes.value: GSM4263538