title: GSM2559476: Hep3B sh AMPK 2# H3K9 Me3; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-IT High Sensitivity kit (Active motif) according to the manufacturer’s manual Prepared using DNA Library preparation kit (KAPA, KK8232), as per the manufacturer’s protocol. In brief, DNA was fragmented to a median size of 150bp by sonication. Fragmented DNA ends were polished and 5′-phosphorylated. After addition of 3′-A to the ends, indexed Y-adapters were ligated and the samples were PCR amplified.
attributes.tag: GEO Accession
attributes.value: GSM2559476