title: GSM3733662: Ago2KO + FM-Ago1 Rep1; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: Total RNA extraction with Trizol according to manufacturer's instructions followed by biochemical purification of Argonaute ribonucleoprotein complexes. Libraries were performed using an in-house protocol: ligate 3p linker; size select ligation product; ligate 5p linker; size select ligation product; reverse transcription; qPCR library amplification. 3p and 5p adapters contain 4N at ligation interface (Jayaprakash et al, NAR, 2011)
attributes.tag: GEO Accession
attributes.value: GSM3733662