title: GSM2551346: 21B Young Combined PreTraining; Homo sapiens; RNA-Seq
library_construction_protocol: We measured mRNA expression as previously described (Lanza et al., Cell Metab. 16 (2012), pp. 777–788) with slight modifications. In brief, total RNA was isolated from the biopsy collected at 1000 hr, and sequencing libraries were prepared with TruSeq RNA Sample Prep Kit v2. Libraries were sequenced on a HiSeq 2000 sequencer using TruSeq SBS sequencing kit version 3 and HCS version 2.0.12.0 software. Genes with a FDR-corrected p value of ≤0.05 and an absolute log2 fold change of ≥0.5 (where 0.0 signifies no change) were considered for further analysis.
attributes.tag: GEO Accession
attributes.value: GSM2551346