title: GSM2883963: 5N; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol® RNA Isolation Reagents. The isolated total RNA was processed for preparing mRNA sequencing library using the Illumina TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA, USA) according to the manufacturer's protocol to have single 8bp indices for multiplexing. Quality and size of libraries were assessed using Agilent 2100 bioanalyzer DNA kit (Agilent, Santa Clara, CA, USA). All libraries were quantified by qPCR using CFX96 Real Time System (Biorad, Hercules, CA, USA)
attributes.tag: GEO Accession
attributes.value: GSM2883963