library_construction_protocol: "Sequencing was performed on the Illumina MiSeq platform using the primers 335Fc and 769Rc. PCR conditions: 98oC for 5 min; 98oC for 10 s / 60oC for 30 s / 72oC for 30 s (20 cycles); 72oC for 5 min. PCR reaction (total 25 ul); 12.5 ul NEB Next High Fidelity Master Mix, 0.5 ul of each primer, 2.5 ul of 3% BSA, 100 to 200 ng of template DNA. Sample purification: Agencourt AMPure XP extraction kit (Beckman Coulter Company), ratio of AMPure XP to PCR reaction is adapted to 0.6 to 1. Indexing PCR: 98oC for 5 min; 98oC for 10 s / 55oC for 30 s / 72oC for 30 s (8 cycles); 72oC for 5 min. Products are pooled equimolar to a final concentration of 4 nM and sequenced using the MiSeq Reagent kit v3 (600 cycles) for paired end sequencing."