title: Illumina HiSeq 2500 sequencing; microRNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
design: microRNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
library_construction_protocol: In vitro culture protocol for human gallbladder cells. Dissociated cells from human gallbladder and cystic duct were cultured in rat mammary feeder culture using a modified culture medium as published in PMID 23562832. Adenoviral transduction of expanded/cultured human gallbladder cells and Reprogramming culture conditions of transduced human gallbladder cells Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol microRNA libraries were generated and processed by the University of Pennsylvania’s Next Generation Sequencing Core using Illumina truSeq small RNA library preparation kit (Illumina, San Diego, CA, USA)
attributes.tag: Experimental Factor: gallbladder cell reprogrammed to beta cell
attributes.value: cell type
attributes.tag: Experimental Factor: Hpi1 negative
attributes.value: phenotype