title: GSM2208754: MEC1,Exo,3; Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA of MEC-1 exosomes as well as parental cells was isolated via the miRNeasy Micro Kit (Qiagen) following manufacturer’s instructions for the isolation of small RNA (< 200nt). Libraries were prepared via the NEBNext Multiplex Small RNA Prep Set for Illumina (Set1) with 30ng input RNA per sample and applying primers diluted 1:2 for RT and 1:1 for PCR, and adapters diluted 1:2. PCR amplification was set to 15 PCR cycles. Libraries were pooled equimolar (2 nM) and sequenced using HiSeq v4 platform (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2208754