title: GSM3374523: WT 2 bio rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Invitrogen) accoding to manufacture instruction. cDNA synthesis was performed with total RNA samples 100ng each using 200 pmol of 5ht2cRTrev oligonucleotide and 10 U of Transcriptor Reverse Transcriptase (Roche) in a 20 µl reaction as described above. Five µl aliquots of the RT-reactions were subjected to 10 cycles of PCR amplification with 5-Ht2cr exons IV and VI specific oligonucleotides: 5ht2cF and 5ht2cRTrev. PCR products were purified using High Pure PCR cleaning Micro Kit (Roche) and eluted in 20 µl of ddH2O. Ten µl of purified reactions were used for 15 cycles of PCR amplification to introduce  PGM adapters with barcodes. Twelve cDNA libraries (6 per genotype) were generated. Choroid plexuses from 24 male mice (12 animals from each genotype) at postnatal day 21 were isolated; two choroid plexuses per library were pooled and subjected to total RNA extraction.
attributes.tag: GEO Accession
attributes.value: GSM3374523