title: GSM4314487: negative control shRNA3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, USA), then RNA concentration, purity and integrity were assessed. Subsequently, mRNA was captured by Dynabeads Oligo (dT)25 (Thermo Fisher Scientific, USA), then first and second strands of the cDNA were synthesized and library fragments were purified using AMPure XP system (Beckman Coulter, USA). PCR assay and measurement of libraries quality were conducted using Bioanalyzer 2100 system (Agilent Technologies, USA), then clustering of index-coded samples was performed using HiSeq PE Cluster Kit v4 cBot (Illumina, USA), and the libraries were sequenced on Illumina Hiseq X10 platform (Illumina, USA) followed by that 150 bp paired-end reads were produced after cluster generation for mRNA.
attributes.tag: GEO Accession
attributes.value: GSM4314487