title: Illumina HiSeq 2500 paired end sequencing; Indentification of Dis3L2 targets in Drosophila melanogaster wing imaginal discs
design: Indentification of Dis3L2 targets in Drosophila melanogaster wing imaginal discs
library_construction_protocol: 60 wing imaginal discs per sample were dissected from wandering 3rd instar larvae. 69B-GAL4 homozygous females were crossed to homozygous UAS-dis3L2RNAi males at 25 degrees C.All offspring were of knockdown (UAS-dis3L2RNAi/+ ; 69B-GAL4/+) genotype.  RNA was extracted from each sample of 60 wing discs using a miRNeasy kit.  RNA was polyA selected then libraries were constructed using an illumina TruSeq RNA sample preparation protocol.
attributes.tag: Experimental Factor: wild-type
attributes.value: genetic modification
attributes.tag: Experimental Factor: w1118 ;; P{GD9240}v51854
attributes.value: genotype