title: 5_SHM of no plasmid-CIT
design: A 447 bp region targeting the 5-S of immunoglobulin heavy chain gene was amplified and followed by index PCR using the Nextera XT Index kit (Illumina, FC-131-1001) according to the manufacturer's protocol (16S Metagenomic Sequencing Library Preparation, 15044223 B JPN, Illumina). The pooled library was sequenced on the Illumina MiSeq platform (2x300bp).