title: GSM2310118: MK1-H3K4me1; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin was extracted from mouse keratinocyte cells (MK1 and MK2) using a low SDS shearing kit (Diagenode: C01020010). Lysates were sonicated with a Bioruptor for 30-40 min. Ips were carried out with Indirect ChIP protocol using 4A4 antibody for p63 and histone antibodies from Diagenode. Libraries were prepared using ThruPLEX DNA-seq kits from Rubicon Genomics. The samples were then 50 bp single-end sequenced on an Illumina HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM2310118