title: GSM2730581: Anp32e_Null_PostZGA_H3K4me1_ChIP-Seq_Rep1; Danio rerio; ChIP-Seq
library_construction_protocol: Whole embryos were cross-linked with 2.2% formaldehyde, then nuclei were extracted as described in Potok et al. Cell 2013. Nuclei preps were then combined and sonicated using a Branson Sonicator. Antibodies were then added to each lysate to bind overnight. The following day protein A/G dynabeads were then used to precipitate antibody bound chromatin (bead binding was for 4 hours at 4C). Bead bound chromatin was then washed and eluted using standard ChIP techniques. NEBNext ChIP-Seq Library Prep Reagents
attributes.tag: GEO Accession
attributes.value: GSM2730581