title: Environmental_16S_Amplicon: CH_DNA_Spring_0.2_Night
design: 16S rRNA gene and 16S cDNA amplifications were performed using forward and reverse barcoded primers specific to the V4 region as described previously for the V4 forward (v4.SN5xx). However, the reverse 16S rRNA gene barcoded primer (v4.SN7xx) was modified where the H was replaced with an N, as suggested previously.