title: Nanopore DNA-seq of Bacteroides fragilis: BF17. Second run. Demultiplexed and trimmed fastq.
design: DNA extraction with Qiagen Genomic Tip g/500.  Library prep: Rapid Barcoding kit (SQK-RPB004) (protocol version RPB_9059_v1_revC_08Mar2018): input DNA 400 ng. This sample: BARCODE 02. (Multiplex of four barcodes: 01,02,03,04). AmPure XP cleanup. FLO-MIN106. MinKNOW/2.2.12. Demultiplexed with deepbinner v0.2.0 (https://github.com/rrwick/Deepbinner) and basecalled using Albacore v2.3.3, retaining only barcodes that deepbinner and Albacore agreed on for minimal barcode misclassification. Porechop v0.2.4 (https://github.com/rrwick/Porechop) with the --discard_middle option was used for adapter and barcode trimming. Run ID: bf17_s01_99377