title: Illumina MiSeq sequencing; qiita_ptid_5535:11950.loc2.MFGypsum.wet.TP2.RE
design: Here, we applied bacterial/viral particle counting, qPCR, 16S and ITS rRNA amplicon sequencing, and metabolomics to longitudinally characterize the ecological dynamics of four commonly used building materials maintained at high humidity conditions
library_construction_protocol: Illumina EMP protocol amplification of ITS1fbc, ITS2r
attributes.tag: barcode
attributes.value: ACCTGGGAATAT
attributes.tag: center_name
attributes.value: ANL
attributes.tag: center_project_name
attributes.value: BE Microbiome
attributes.tag: linker
attributes.value: CG
attributes.tag: linkerprimer
attributes.value: CGGCTGCGTTCTTCATCGATGC
attributes.tag: pcr_primers
attributes.value: FWD:CTTGGTCATTTAGAGGAAGTAA; REV:GCTGCGTTCTTCATCGATGC
attributes.tag: primer
attributes.value: CGGCTGCGTTCTTCATCGATGC
attributes.tag: run_center
attributes.value: ANL
attributes.tag: run_prefix
attributes.value: run file
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: target_gene
attributes.value: ITS
attributes.tag: target_subfragment
attributes.value: ITS1/468