title: AccuNGS_rpob
design: DH5a bacteria; RpoB PCR amplicon, 40 PCR rounds with Superfi Platinum polymerase; PCR product extraction from agarose gel; Nextera XT library preparation kit with 12 PCR rounds using Superfi Platinum polymerase; library purification and size selection with AMPure XP beads; paired-end sequencing with Illumina MiSeq