title: AccuNGS_alt_pcr_purification(exosap)
design: pLAI.2 plasmid grown in DH5a and purified; Integrase PCR amplicon, 40 PCR rounds with Superfi Platinum polymerase; PCR product cleanup with Exosap; Nextera XT library preparation kit with 12 PCR rounds using Superfi Platinum polymerase; library purification and size selection with AMPure XP beads; paired-end sequencing with Illumina MiSeq