title: GSM3523318: Illumina.UNT_9576; Mus musculus; RNA-Seq
library_construction_protocol: Mice were sacrificed and liver samples were dissected and snap-frozen in liquid nitrogen. RNA was extracted from the liver tissue using TRIzol and Qiagen Rneasy Kit, according to manufacturers' protocols. Illumina TruSeq: Total RNA from each liver sample was prepared for sequencing using the Illumina: TruSeq Stranded mRNA Sample Preparation Kit. 200 ng of RNA was used to prepare the RNA libraries. The libraries were sequenced on Illumina HiSeq 2500. Depths of 16-34 million paired-end 125 bp reads were generated for each sample. (GSE98562)    Takara Bio SMARTer: Total RNA from each liver sample was prepared for sequencing using the Takara Bio SMARTer: SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian. 1.7-2.6 ng of RNA was used to prepare the RNA libraries. The libraries were sequenced on Illumina HiSeq 4000. Depths of 30-44 million paired-end 150 bp reads were generated for each sample. The libraries were reproduced to repeat the experiment and ensure reproducibility.    Takara Bio SMART-Seq: Total RNA from each liver sample was prepared for sequencing using the Takara Bio SMART-Seq: SMART-Seq® v4 Ultra® Low Input RNA Kit. 0.8-1.3 ng of RNA was used to prepare the RNA libraries. The libraries were sequenced on Illumina HiSeq 4000. Depths of 15-30 million paired-end 150 bp reads were generated for each sample.
attributes.tag: GEO Accession
attributes.value: GSM3523318