title: GSM2310333: Scer_TGIRT_DMS_denatured_genomeWide; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA was extracted using hot acid phenol. mRNA was randomly fragmented using Ambion fragmentation reagents, followed by PNK, and ligation using T4 K227Q truncated RNA ligase. rRNA was depleted post-ligation using RiboZero (Epicentre), and reverse transcription was done with the TGIRT enzyme. Circ ligase (Epicentre) was used for circularization, and Illumina adapters were introduced by 8-10 cycles of PCR. Gene specific RT/PCR followed by Nextera XT or LMPCR. For genome-wide libraries we used 3' ligation, followed by circularixation, and PCR to add the sequencing adapters.
attributes.tag: GEO Accession
attributes.value: GSM2310333