title: Small RNA sequences of Meloidogyne incognita: egg stage
design: Total RNA was isolated at each devlopmental stage of the nemtode life cycle and from the isolated total RNA, nucleotides of length 18- 30 bp were obtained by gel separation and the purified short nucleotide RNA sequences were ligated with adapters (3′ adapter 6-20nt and 5′adapter 12-25 nt) at both ends which was followed by purification according to the manufacturer’s protocol (T4 RNA Ligase, 200 U, 30 U/ul, Takara: D2050). Ligated small RNAs were reverse transcribed with the complimentary sequence of 3′ adaptor (SuperScriptH III Reverse Transcriptase, Invitrogen: 18064-014), and amplified. Finally, the PCR amplification products were sequenced at BGI (Beijing Genome Institute) using Illumina HiSeq 2000.