title: Toxicant responsive transcriptome of the diatom Ceratoneis closterium, formerly Nitzschia closterium
design: The aims of this study were to 1) describe the transcriptome of the diatom Ceratoneis closterium, both to serve as a reference species in future RNA seq projects, as well as to enable the construction of microarrays and qPCR assays and 2) record changes in the transcriptome when the diatom is exposed to contaminants.  Cells were grown under control conditions for 96 h, RNA was extracted using a TRIzol procedure and qiagen RNeasy clean up.  Messenger RNA was concentrated using the invitrogen Ribominus kit for plants.