title: Illumina HiSeq 2000 sequencing; ChIP-seq of Stat3 with DNA binding sites mutation in human colon cancer SW480
design: ChIP-seq of Stat3 with DNA binding sites mutation in human colon cancer SW480
library_construction_protocol: SW480 cells were edited by CRISPR-CAS9 for the residue 400 to 411 (\"NNGSLSAEFKHL\") of Stat3 deletion.  10% whole cell lysates were saved as input after genomic DNA was broken into 200-500 bp by sonication. 1 μg IP grade antibodies of STAT3 were incubated with the rest of the lysate overnight, followed by 2 h protein-A beads incubation at 37 °C for target protein pull down. The STAT3 enriched DNA or input DNA were repaired to 3'-dA overhang and added the ligated adapter.  0.2 ug of pull down DNA sample was submitted to NOVOGENE (Beijing, China) for library generation and sequencing.
attributes.tag: Experimental Factor: genotype
attributes.value: STAT3 knockout
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: anti-STAT3