title: GSM2310168: BS-seq_H9_hEpiLC_d4_1; Homo sapiens; Bisulfite-Seq
library_construction_protocol: WGBS: DNA was isolated using Beckman Coulter's Agencourt RNAdvance Cell v2 kit and used for whole-genome bisulfite (WGBS) libraries (Seisenberger et al., 2012). Briefly, WGBS libraries were prepared by sonicating genomic DNA using a Covaris Sonicator, followed by end-repair, A-tailing and methylated adapter (Illumina) ligation using NEB-Next reagents. Subsequently, libraries were bisulfite treated using EZ DNA Methylation-Direct Kit (Zymo), followed by library amplification with indexed primers using KAPA HiFi Uracil HotStart DNA Polymerase (KAPA Biosystems). All amplified libraries were purified and assessed for quality and quantity using High-Sensitivity DNA chips on the Agilent Bioanalyzer. High-throughput sequencing of all libraries was carried out with a 125 bp paired-end protocol on a HiSeq 2000 instrument (Illumina). Genomic DNA and total RNA were isolated from the samples using the Beckman Coulter's Agencourt RNAdvance Cell v2 kit following the manufacture’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2310168