title: RNA-seq of Morus alba in time 3-1
design: For each sample, TRIzol Reagent (Tiangen Biotech Co., Ltd., Beijing, China) was used to extract RNA following the protocol provided by the manufacturer. After cDNA synthesis, samples were subjected to phosphorylation, A base addition, and end-repair according to library construction protocol. Sequencing adapters were then added to both sizes of the cDNA fragments. After PCR amplification of cDNA fragments, the 150-250 bp targets were cleaned up.