title: GSM2209612: LUCH lil-2_r3 (XC); Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Ten-day-old seedlings were used for RNA extraction using Trizol (Invitrogen, 15596– 018), and the extracted RNA was treated with DNase I (Roche, 04716728001). For Col,  mbd7-3 and mbd7-4, RNA-seq libraries were generated using 2.0 ug of DNaseI-treated RNA and the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Cat# E7530) according to the manufacturer’s instructions. For YJ, YJ lil-1, LUCH, and LUCH lil-2, Two micrograms of the DNase I- treated RNA were used for RNA-seq library construction with the TruSeq RNA Sample Preparation Kit v2 (Illumina, FC-122-1002). The libraries were sequenced on an Illumina HiSeq 2000 instrument at the genomics core facility at UC Riverside.
attributes.tag: GEO Accession
attributes.value: GSM2209612