title: GSM2462970: 60 hours in culture expt 1 #2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared from cells using RNAzol, DNase treated and purified using RNA clean and concentrator columns. 1 ug of total RNA was subsequently used for rRNA depletion using the RiboZero depletion kit. RNA-Seq library was prepared by using the TruSeq RNA sample prep kit (Illumina) according to manufacturer’s instructions. 50-cycle single-end RNA sequencing was performed on a HiSeq 4000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2462970