title: GSM3465912: met1_input_ChAP; Arabidopsis thaliana; OTHER
library_construction_protocol: ChAP experiments were performed according to previous publication with minor modifications (Zilberman et al. 2008)ChAP experiments were performed according to previous publication with minor modifications (Zilberman et al. 2008).4-week-old roots were vacuum infiltrated in 1% formaldehyde solution for 15 minutes to cross-link the chromatin. Roots were ground in liquid nitrogen and nuclei were isolated as described (Zilberman et al. 2008). The nuclear suspension was fragmented by sonication to 0.5-2 kb then 90% of biotinylated protein-containing chromatin was affinity purified using streptavidin beads. 10% of the sheared chromatin suspension was used as input control. Reverse cross-linking was performed with 5M NaCl and the DNA was purified as described (Zilberman et al. 2008)..4-week-old roots were vacuum infiltrated in 1% formaldehyde solution for 15 minutes to cross-link the chromatin. Roots were ground in liquid nitrogen and nuclei were isolated as described (Zilberman et al. 2008). The nuclear suspension was fragmented by sonication to 0.5-2 kb  then 90% of biotinylated protein-containing chromatin was affinity purified using streptavidin beads. 10% of the sheared chromatin suspension was used as input control. Reverse cross-linking was performed with 5M NaCl
attributes.tag: GEO Accession
attributes.value: GSM3465912