title: GSM2042674: RiboMethSeq_HEK_totalRNA_14min; Homo sapiens; OTHER
library_construction_protocol: total RNA was extracted using TRI Reagent (#T9424), poly(A)+ RNA was enriched using Dynabeads mRNA direct kit (# 61012), according to manufacturer's instructions. Nuclear and cytoplasmic RNA fraction were collected with the REAP method (PMID: 21067583) Libraries were prepared following the protocol of Birkedal et al. (PMID: 25417815) using Illumina-compatible adapters and primers. After adapter ligation DNA was PCR amplified with Illumina primers for 16 cycles and library fragments of ~140-160 bp (insert plus adaptor and PCR primer sequences) were band isolated from an non-denaturing polyacrylamide gel. The purified DNA was captured on an Illumina HiSeq2500 flow cell for cluster generation. Libraries were sequenced following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM2042674