title: RNA-seq of KCL22 (human cellline) untreated
design: RNA was extracted using an RNeasy Mini kit (Qiagen, Cat no. 74104) as per the manufacturerŐs instructions followed by RNA enrichment using polyA selection. The sequence library was created using the TruSeq RNA Library Prep version 2 kit, to polyA select mRNA. The library insert size was ~300bp. The libraries were sequences on the Illumina NextSeqŞ500 platform with ~50 million paired end reads per sample. The sequences were trimmed using cutadapt v 1.0 (quality value=25, minimum read length=56). The resulting reads were aligned to the human genome GRCh37 (p13) using HiSat2 v 2.0.4 and mapped to Ensembl gene IDs.