title: Illumina MiSeq sequencing; qiita_ptid_5536:11950.loc2.Plywood.dry.TP1.RE
design: Here, we applied bacterial/viral particle counting, qPCR, 16S and ITS rRNA amplicon sequencing, and metabolomics to longitudinally characterize the ecological dynamics of four commonly used building materials maintained at high humidity conditions
library_construction_protocol: Illumina EMP protocol 515fbc, 806r amplification of 16S rRNA V515
attributes.tag: barcode
attributes.value: ATACTCGGCTGC
attributes.tag: center_name
attributes.value: ANL
attributes.tag: center_project_name
attributes.value: BE Microbiome
attributes.tag: linker
attributes.value: GT
attributes.tag: linkerprimer
attributes.value: GTGTGYCAGCMGCCGCGGTAA
attributes.tag: pcr_primers
attributes.value: FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGYCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: ANL
attributes.tag: run_prefix
attributes.value: Sloan1_16S_S0_L001
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4