title: GSM2310799: ATCC55737_rep2; Acidipropionibacterium acidipropionici; RNA-Seq
library_construction_protocol: Cells sampled at a mid-exponential phase of instrumented fermenters were used for RNA extraction. Briefly, 50 ODs were harvested and centrifuged at 4000g for 10 min at room temperature. The supernatant was removed, and five mL of RNA later reagent was added to the pellet. After 8-24 h of incubation at 4 oC, the RNA later was removed by centrifugation and the pellet stored at -80 oC for further use. RNeasy® Mini Kit (Qiagen) was used to extract the RNA and the RNA Clean and Concentrator-25 Kit (Zymo) to clean it. Next, the RNA was enriched depleting the ribosomal RNA with the Ribbo-Zero Magnetic Kit (Illumina). The samples were cleaned and concentrated with the RNA Clean and Concentrator-5 Kit (Zymo). The quality of the RNA was evaluated by a Bioanalyzer. Illumina standard protocols
attributes.tag: GEO Accession
attributes.value: GSM2310799