title: Illumina HiSeq 2000 sequencing; qiita_ptid_64:1883.2008.413.Crump.Artic.LTREB.main.lane2.NoIndex
design: A 12-year sample archive of DNA from Alaskan arctic tundra ecosystems are available to the Earth Microbiome Project for comparison of microbial diversity across a range of arctic environments from soils to water to sediments. These samples were preserved and extracted using a method that differs from that of the EMP. Therefore, we must test how the extraction method used to extract our archived samples compares with those methods used by the EMP. The samples for this test were collected during the summer of 2011 at the Toolik Field Station. Each site was sampled in triplicate and samples were divided and preserved for extraction using our standard protocol (three replicates), and for extraction using the EMP protocol (three replicates). Half of the samples are from the epilimnion and hypolimnion of ultra-oligotrophic Toolik Lake collected over the course of the summer, and the rest are from different environments associated with a headwater stream that lies upslope of Toolik Lake. The data from these samples will allow us to test whether our extraction protocol yields results that are similar to the EMP extraction protocol, to investigate how replicates compare, and to determine how the microbial community and its metagenome changes over the course of the summer in Arctic tundra waters.
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: ACTTCCAACTTC
attributes.tag: center_name
attributes.value: CCME
attributes.tag: center_project_name
attributes.value: Crump_Arctic_LTREB_main
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: HiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 2/1/14
attributes.tag: run_prefix
attributes.value: Crump_Artic_LTREB_main_lane2_NoIndex
attributes.tag: samp_size
attributes.value: 0.1, gram
attributes.tag: sample_center
attributes.value: University of Maryland
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4