title: Whole exome analysis of Homo sapiens: HTT CAG repeat expansion carriers from the PREDICT-HD study of Huntington's disease: Sample 13240_9
design: "An in-solution DNA probe based hybrid selection method that uses similar principles as the Broad Institute-AgilentTechnologies developed in-solution RNA probe based hybrid selection method (for details please see PMID 19182786 and 21205303) was used to generate Illumina exome sequencing libraries. The Illumina exome specifically targets approximately 37.7Mb of mainly exonic territory made up of all targets from Agilent SureSelect All Exon V2, all coding regions of Gencode V11 genes, and all coding regions of RefSeq gene and KnownGene tracks from the UCSC genome browser (http://genome.ucsc.edu). Pooled libraries were normalized to 2nM and denatured using 0.2 N NaOH prior to sequencing. Flowcell cluster amplification and sequencing were performed according to the manufacturer's protocols using the  HiSeq 2500.  Each run was a 76bp paired-end with a dual eight-base index barcode read. Data was analyzed using the Broad Picard Pipeline which includes de-multiplexing and data aggregation."
attributes.tag: alignment_software
attributes.value: BWA