title: Illumina MiSeq sequencing of rumen microbe
design: In this study, we investigated the predominant ureC genes of the ureolytic bacteria in the rumen of dairy cows using high-throughput sequencing. Six dairy cows with rumen fistulas were assigned to a two-period cross-over trial. One group (Ctrl; n = 3) were fed the diet without urea and the other (urea; n = 3) were fed rations plus 180 g urea per cow per day at three separate times. Rumen bacterial samples from liquid, solid and rumen wall fractions were collected for ureC gene amplification and sequencing using Miseq.