title: GSM4261393: ATAC_seq_EC_ZT10_WT_rep2; Mus musculus; ATAC-seq
library_construction_protocol: 50,000 isolated ECs or KCs were resuspended in 50 μl of transposase mixture (25 μl of 2× TD buffer, 2.5 μl of TDE1, 0.5 μl of 1% digitonin, and 22 μl of nuclease-free water) (15027865, Illumina). Transposition reactions were incubated at 37 °C for 30 min in an Eppendorf Thermo Mixer with agitation at 300 rpm. Transposed DNA was purified using a QIAGEN MinElute Reaction Cleanup kit (28204), and purified DNA was eluted in 10 μl of elution buffer (10 mM Tris-HCl, pH 8). Transposed fragments were amplified and purified as described previously (PMID: 25559105).
attributes.tag: GEO Accession
attributes.value: GSM4261393