title: GSM2209886: CTCF_ChIP-seq_Asynchronous; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were washed with cold PBS(-), and kept on ice for 5 min in a cold CSK buffer (10 mM PIPES-NaOH, pH 6.8, 100 mM NaCl, 3 mM MgCl2, 1 mM EGTA, 300 mM sucrose, 0.1% Triton X-100, 1 mM Na3VO4, 1 mM NaF, 5 mM beta-D-glycerophosphate).  After centrifugation at 2,300 ×g for 5 min, pellet fractions were incubated at 25°C for 10 min in CSK buffer containing 0.5% formaldehyde, and then incubated with 25°C for 5 min in 0.125 M glycine/PBS.  Immunoprecipitation assays were carried out according to the manufacture’s protocol (Chromatin Immunoprecipitation Assay Kit; Millipore). Libraries were prepared according to Thermo fiser's instructions accompanying the 5500 SOLiD Fragment Library Core Kit (#4464412).
attributes.tag: GEO Accession
attributes.value: GSM2209886