title: Targeted sequencing of human: blood DNA
design: Random subsampling by seqtk 1.0-r31, adapter trimming and quality filtering by Trimmomatic 0.35, mapping to hg19 by BWA 0.7.12, SAM to BAM conversion, fixmate and sort by Samtools 1.4.1, duplicate removal by Picard 2.17.10, base recalibration and local realignment by GATK 3.7.