title: GSM2309782: 8 hpl Replicate 2; Danio rerio; RNA-Seq
library_construction_protocol: Retinas were removed and dissociated to obtain single-cell suspensions. Müller glia were collected with fluorescently-actived cell-sorting. RNA was isolated with TRIzol LS according to recommenders protocol. RNA quality was checked via BioAnalyzer and samples with an RNA integrity number (RIN) > 7.0 were used for RNA-seq library construction. Stranded RNA-seq libraries were constructed using a modification of the Illumina TruSeq mRNA (Brooks, et al. Meth Mol Biol 2012). Each library was single-end sequenced in an independent lane of a GAIIx. Fastq files were generated from reads passing chastity filter.
attributes.tag: GEO Accession
attributes.value: GSM2309782