title: Primary transcriptome of Rhodobacter capsulatus
design: Primary transcriptome of Rhodobacter capsulatus SB1003 during exponential growth in minimal media under microaerobic conditions. DNA free RNA was treated with TEX to enrich primary transcripts and fragmented (~200nt) without prior rRNA depletion. Illumina cDNA libraries resulting from treated RNA were generated by 5-phosphorylation, ligation of 3- and 5-RNA-adapter oligonucleotides, and reverse transcription as described in Nuss et al. (2015) PLoS Genet. 11(3): e1005087. After reverse transcription, cDNA libraries were PCR amplified (15 cycles). cDNA libraries were sequenced on a HiSeq 2500 machine in single-read mode running 100 cycles with Genome Analyzer IIx (standard protocol).