title: Ion Torrent Proton sequencing; Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinylestradiol
design: Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinylestradiol
library_construction_protocol: RNA extraction Whole testis homogenized in TriReagent according to the manufacturer (0.8 ml/sample) (Sigma-Aldrich, Germany) EE2 exposure. Fish exposed to 0, 1.2 or 1.6 ng/L EE2 for 80 days starting 1 day after fertilization   RNA extraction Whole testis homogenized in TriReagent according to the manufacturer (0.8 ml/sample) (Sigma-Aldrich, Germany) Ion Whole transcriptome analysis kit on RiboZero (eukaryote) depleted total RNA
attributes.tag: Experimental Factor: compound
attributes.value: none