title: GSM2461850: SH4_YZ4_KO1_1344_06_19_14; Mus musculus; RNA-Seq
library_construction_protocol: The somach was removed, frozen (unfixed) in OCT with isopentane cooled with liquid nitrogen, sectioned, and the neck region captured by laser capture microdissection.  RNA was made using the RNeazy microkit (Qiagen; #74004).  RNA quantity was determined on the Qubit using the RNA BR Assay kit (ThermoFisher; #Q10210)).  RNA quality was determined on the Bioanalyzer using the RNA pico kit (Agilent; #5067-1513).  100 ng of RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM2461850