title: GSM4485089: Samples #20; Mus musculus; RNA-Seq
library_construction_protocol: RNA was quantified and mixed at 10ng/µl. Total RNA was quantified using the Qubit 2.0 fluorimetric Assay (Thermo Fisher Scientific). Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit  (Lexogen, Vienna, Austria) according to manufacturer's instructions The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the NovaSeq 6000  (Illumina) with a read length of 100 bp. Quality of libraries was assessed by using screen tape High sentisivity DNA D1000 (Agilent Technologies).
attributes.tag: GEO Accession
attributes.value: GSM4485089