title: GSM3223401: sc20_3: Scrambled siRNAs, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: 72 hours post transfection, total RNA was extracted with the RNEasy (Qiagen) kit from 25,000 cells per sample. Either ERCC mix1 or mix2 external standards (Invitrogen) were diluted 1:100 in water and 1 ul was added to each lysate. Libraries were prepared from 100 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer.
attributes.tag: GEO Accession
attributes.value: GSM3223401