title: GSM2309315: U2AF65-knockdown MCF-7 cells; Homo sapiens; RNA-Seq
library_construction_protocol: 10 μg of total RNA was treated with 1 U RQ1 DNase (Promega) to remove residual DNA. 10 μg of total RNA was used for RNA-Seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Thermo Fisher). Prior to directional RNA-seq library preparation, the purified mRNAs were fragmented with ion at 95°C, followed by end repair and 5' adaptor ligation. Reverse transcription was then performed with primers harboring a 3' adaptor sequence and randomized hexamers. cDNA was purified and amplified, and PCR products corresponding to 200-500 bp were purified, quantified and stored at -80°C until sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2309315