title: GSM3523270: Spt6LΔYqgF-GFP spt6l heterozygous IP rep1; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: ChIP was performed as previously described (Chen et al., 2017) with some modifications. Five grams of 10-day-old Arabidopsis seedlings grown on ½ MS medium were collected. Protein A Dynabeads (Invitrogen) was pre-incubated with antibody (5 µL for 50 µL beads) at 4 degree in a rotor at least 6h. After removing excess or unbound antibodies, the pre-cleaned chromatins (cleaned by incubating with Dynabeads alone) were add into antibody bound Dynabeads. NEBNext® Ultra™ DNA Library Prep Kit (E7370S)
attributes.tag: GEO Accession
attributes.value: GSM3523270