title: Illumina HiSeq 2500 paired end sequencing; Molecular basis of beige adipocyte maintenance
design: Molecular basis of beige adipocyte maintenance
library_construction_protocol: 1mg/kg for 7 consecutive days Total RNA was extracted from FACS-isolated GFP+ adipocytes or preadipocytes using the RNeasy Micro Kit (Qiagen). RNA quality (RIN: RNA Integrity Number) was determined by Bioanalyzer (Agilent Technologies) and samples with RIN of >7.0 were subjected to RNA-seq. Libraries were constructed from minimum of 22 ng of total RNA as previously described (Nature 504 (7478), 163-167)
attributes.tag: Experimental Factor: preadipocyte
attributes.value: cell type