title: GSM2043228: X; Vitis vinifera; Bisulfite-Seq
library_construction_protocol: Furthermore, in order to capture a representative biological selection of transcripts at each time-point, RNA for Illumina sequencing was purified from tissue consisting of 20 berries collected from 10 bunches. Total DNA was extracted from the fruits collected from our experiments. Total DNA was prepared by proteinase K/phenol extraction following the manufacturer’s instructions. The DNA was fragmented by sonication using a Bioruptor (Diagenode, Belgium) to a mean size of ~250bp, followed by the blunt ending, dA addition to 3’-end, and adaptor addition following the manufacturer’s protocol. The bisulfite conversion of the adaptor-added DNA was performed as described previously (Hayatsu, Tsuji et al. 2006). Ultra-high-throughput pair-end sequencing was performed using the Hiseq 2000 according to the manufacturer’s instructions. Raw data were processed using Illumina base-calling pipeline.