title: Illumina HiSeq 2000 paired end sequencing; Comparison of root transcriptomes in four soybean cultivars with high and low isoflavonoid content
design: Comparison of root transcriptomes in four soybean cultivars with high and low isoflavonoid content
library_construction_protocol: Two week-old soybean roots were harvested, flash-frozen with liquid nitrogen and ground to a fine powder Planted in Pro-Mix BX MycorrhizaeTM soil (Rivière-du-Loup, Canada) in a growth chamber with a 16 h light cycle at 25°C, and an 8 h dark cycle at 20°C, with 60-70% relative humidity, with light intensity 100-150 μmol m-2s-1. RNA was extracted from the twelve samples according to the instructions in the RNeasy Mini Kit (Qiagen, Germany). RNA integrity and concentration were recorded using the Agilent 2100 (Bioanalyzer, USA). A total of 4 µg of RNA, at a concentration of  approximately 100 ng/µL was used per replicate Library constructed from soybean root RNA
attributes.tag: Experimental Factor: 1.4
attributes.value: metabolic process