title: Illumina HiSeq 2000 sequencing; RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
design: RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
library_construction_protocol: The islets were not cultured. High purity intact human islets were obtained from the Integrated islet distribution program (IIDP).  To isolate the beta cells, islets were enzymatically dissociated into single cells and stained using surface monoclonal antibodies and sorted using FACS following published protocols (pmid:19383399 and27399229). The FACS-sorted beta cells were collected and processed for total RNA isolation. Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol TruSeq RNA sample library preparation v2 as recommended by the manufacturer Illumina
attributes.tag: Experimental Factor: beta cell
attributes.value: cell type
attributes.tag: Experimental Factor: Hpi1 positive
attributes.value: phenotype