title: GSM1233302: DSN_NB4_2392_24hrnaseq; Homo sapiens; RNA-Seq
library_construction_protocol: Chromatin was sonicated and used for ChIP-seq using H3K9K14ac (Diagenode). RNA was extracted using qiagen RNeasy kit according to manufacturer. cDNA first and Ds-cDNA synthesis then, was  performed following supplier’s instructions (Invitrogen). Ds-cDNA samples were prepared for  sequencing by end repair of 20 ng DNA as measured by Qubit (Invitrogen). Adaptors were ligated to DNA fragments, followed by size selection (~300 bp) and limited PCR amplification (14 cycles). The resulting sample libraries were subjected to DSN treatment (Evrogen) according to the manufacturer’s recommendations. ChIP and RNA seq libraries were preapared according to  Illumina's standard protocol.
attributes.tag: GEO Accession
attributes.value: GSM1233302