title: GSM3523614: RNAseq1_p53WT_ML_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Qiagen RNeasy mini columns including on-column DNase I digestion. mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and library preparation was performed with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina following the manufacturer's instructions. Libraries were size-selected using Agencourt AMPure XP Beads (Beckman Coulter) followed by amplification with 12 PCR cycles. Library quantification and size determination was performed with an Experion automated electrophoresis system (Bio-Rad) and libraries were sequenced with the Illumina NextSeq500.
attributes.tag: GEO Accession
attributes.value: GSM3523614