title: Sarcocheilichthys sinensis Raw sequence reads
design: The Magnetic Oligo (dT) Beads (Invitrogen, USA) was applied to isolate poly (A) mRNA from total RNA. And the mRNA was randomly fragmented by the fragmentation buffer. Using these fragments as templates, cDNA was synthesized and then purified using AMPure XP beads (Beckman, USA). After end reparation and single nucleotide A (adenine) addition for the purified cDNA, adapters were connected. Suitable fragments were selected by AMPure XP beads as templates for PCR amplification and the library was then obtained.