title: T74
design: Total RNA was extracted from the collected samples using EASYspin total RNA Extraction Kit (Biomed, Beijing, China). RNA quality were monitored by gel electrophoresis (1%) and the measurement of A260/A280 (NanoDrop 2000, Thermo). RNA purity was examined using the NanoPhot-ometer spectrophotometer (IMPLEN, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2109 system (Agilent Technologies, CA, USA).