title: Poly A enriched RNA-Seq of wildype BY4741 Saccharomyces cerevisiae strain: YPD, replicate 1
design: Poly A+ libraries were generated from tota RNA using the TruSeq Stranded mRNA Kit (Illumina, RS-122-2102) according to manufacturers instructions. The poly A enriched RNA was then used for strand specific library construction utilizing the TruSeq Stranded Total RNA LT (Illumina, RS-122-2302). The libraries from 8 samples were pooled together and run on a single lane of HiSeq2500 machine for sequencing 50bp single end reads.