title: GSM1666313: cerebellum_E18 (sRNA-seq); Mus musculus; ncRNA-Seq
library_construction_protocol: Tissue total RNA samples for cerebellum and testis were purchased from Zyagen. Total RNA of H1 cells was isolated using Trizol (Life Technologies). Additional column purification and DNaseI treatment were applied using Direct-zol RNA kit (Zymo Research). Spike-in RNAs (Exiqon) were added into 1µg total RNA before library construction for the normalization control between tissue samples. Small RNA sequencing libraries were generated using NEBNext Small RNA library Prep kit and NEBNext multiplex oligos for Illumina according to the manufacturer’s instructions (NEB). The final libraries were purified from 6% PAGE gel, and their concentrations were measured using Qubit fluorometric assay (Life Technologies).
attributes.tag: GEO Accession
attributes.value: GSM1666313