title: 16S amplicon Illumina sequencing of honeybee gut after antibiotic exposure
design: PCR amplifications of the V4 region of the 16S rRNA gene were performed in triplicate using 515F and 806R primers. Reaction products were purified with AMPure XP Beads (Beckman Coulter). The resulting amplicons were subjected to Illumina sequencing on the MiSeq platform (2x250 sequencing run) at the Genome Sequencing and Analysis Facility at the University of Texas at Austin.