title: 16S V4 amplicon sequencing of soil metagenome in old trial Ukulinga low nitrogen addition and phosphate addition plot 2
design: Amplicon libraries were prepared using primers targeting the V4 region of the prokaryotic 16S rRNA gene (F-515-GTGCCAGCMGCCGCGGTAA, R-806-GGACTACHVGGGTWTCTAAT)  modified with heterogeneity spacers and Nextera XT kit (Illumina). Indexed products of both PCR lines were pooled. Sequencing was performed on an Illumina MiSeq with 2 x 300 bp.