title: EdUseq-HU of U2OS cells overexpressing cyclin E for 2-3 days: 6 h after mitotic shake-off treated with mimosine (replicate 2)
design: U2OS cells overexpressing cyclin E were synchronized by mitotic shake-off and released into media containing HU, mimosine and EdU for 6 h before being collected. The EdU labeled DNA was then isolated and sequenced.