title: GSM1665759: 03_FN Animal F Normal; Oryctolagus cuniculus; RNA-Seq
library_construction_protocol: Rabbits were euthanized following anesthesia by a lethal i.v. injection of 3ml Euthasol (Virbac, Fort Worth, TX). In order to maintain the in vivo transcription profile, tissues for RNAseq analysis were transferred to RNAlater (Ambion, Life technologies, Grand Island, NY, USA) within 10 minutes of euthanasia.  After 24h in RNAlater, tissue samples were frozen at -80°C. RNA was then extracted by bead beating with 3mm beads in Trizol according to the manufacturer’s instructions and spin purified on columns (Qiagen, Valencia, CA, USA). Finally, the RNA was treated with AMBION TURBO DNA-free DNase (Life Technologies, Grand Island, NY). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and mRNA was enriched using the RIBOMinus kit according to the manufacturer's instructions (Life Technologies, Grand Island, NY). Successful removal of rRNA was verified by a second Bioanalyzer assay. mRNA libraries were prepared for sequencing and barcoded using standard ABI SOLiD protocols
attributes.tag: GEO Accession
attributes.value: GSM1665759