title: Metagenome from Okhla Landfill
design: The paired-end sequencing libraries were prepared using IlluminaTruSeq Nano DNA Library Prep Kit. Approx-imately 200ng of metagenomic DNA was fragmented by Covaris M220 to generate a mean fragment distribu-tion of 400bp. Covaris results in the generation of ds DNA fragments with 3 or 5 overhangs. The fragments were then subjected to end repair. This ensured low rate of chimera formation. The ligated products were size selected using AMPure XP beads. The size selected products were PCR amplified. The PCR enriched libraries were analyzed on 4200 Tape station system (Agilent Technologies).  The paired end Illumina libraries were se-quenced onto NextSeq500 for cluster generation and sequencing.