title: GSM2553082: DamPc_aly_Rep1; Drosophila melanogaster; OTHER
library_construction_protocol: Testes were collected from 3day-old males. For each biological replicate, 50 pairs of testes were used. Standard phenol-chloroform extraction method was used to isolate genomic DNA from the collected material. 0.5-1 ug DNA was used in each DamID experiment. Genomic DNA was digested by DpnI endonuclease. Digested DNA was ligated with double-stranded DNA adapters and then digested with DpnII endonuclease. Next, methylated fragments were selectively amplified using adapter-specific primer. Before the preparation of libraries for Illumina DamID-Seq, the adapters used in DamID procedure were cut with DpnII enzyme. No further fragmentation was performed. One microgram of genomic DNA was processed for library preparation using the DNA TruSeq kit. The libraries were sequenced on the Illumina MiSeq system.
attributes.tag: GEO Accession
attributes.value: GSM2553082