title: GSM4266002: TASC_DEEP, panel 3 (lung cells); Mus musculus; RNA-Seq
library_construction_protocol: Lungs were perfused with cold PBS and instilled with collagenase I. Lungs were removed while trachea was clamped. The lungs were cleaned from non-respiratory tissue and minced. Following depletion of erythrocytes, mesenchymal cells were sorted as Dapi–/Epcam–/CD45–/CD31–/Pdgfra+/Sca1–/Npnt+ TAP-seq, 10x genomics based Libraries were prepared according to DropSeq protocol (Macosko Cell 2015) or 10X Genomics single-cell RNAseq v2 (Chromium 3' Reagent Kits v2 User Guide Rev C "CG00052") for whole transcriptome or TASC-seq protocol for targeted transcriptome readouts.
attributes.tag: GEO Accession
attributes.value: GSM4266002