title: GSM3374919: LR3; Sus scrofa; Bisulfite-Seq
library_construction_protocol: Total Genomic DNA (gDNA) were extracted from the same frozen tissue samples by using the All Prep DNA Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. DNA concentration was detected by NanoDrop spectrophotometer and quality was assessed by 1% agarose gel by electrophoresis. DNA was fragmented by using sonication into 100-500 bp in size, and then followed by end blunting, dA addition at the 3'end and ligation of adapters. The adapter sequence had multiple methyl cytosines so as to allow monitoring of the bisulfite conversion efficiency. Unmethylated cytosines were converted to uracils after bisulfite treatment by a modified protocol from previously described. DNA fragments with size range of 320 to 380 bp were gel-purified for sequencing. All procedures were carried out by the manufacturer's instructions. Converted DNA was subjected to 50 bp paired end sequencing by Illumina Solexa GA sequencer (Illumina, San Diego, CA, USA). All the raw data was processed by the Illumina Pipeline v1.3.1 (Illumina, San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM3374919