title: GSM3733670: HeLa: input DNA; Homo sapiens; ChIP-Seq
library_construction_protocol: Approximately 1 x 107 cells were used for each ChIP-Seq assay. The chromatin DNA precipitated by polyclonal antibodies against Snail, MTA1, or PRMT5. The DNA was purified with a Qiagen PCR purification kit Libraries were prepared according to Illumina's instructions. The NEBNext Ultra DNA Library Prep Kit for Illumina was used for DNA library construction.  After adapter ligation DNA PCR was amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from the agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced  following the manufacturer protocols.
attributes.tag: GEO Accession
attributes.value: GSM3733670