title: GSM2883181: OX40-2; Mus musculus; RNA-Seq
library_construction_protocol: Double-negative regulatory T cells (DNT) were converted from naïve CD4 T by standard protocol which have been reported before [BLOOD, 1 MAY 2007   VOLUME 109, NUMBER 9].Then CD3+TCRαβ+NK-CD4-CD8- DNT were sorted by FACS Aria II, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2883181