title: GSM3527317: RNA-seq of ConvD [Sample 1-1]; Mus musculus; RNA-Seq
library_construction_protocol: Cells enriched by flow cytometry were directly collected in RNA extraction buffer (RLT-buffer supplemented with beta-Mercaptoethanol) Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 22 ng (Sample 1 and 2) or 1ng (Sample 3 and 4) total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Sample 1 and 2) or NEBnext Low Input kit for Illumina (Sample 3 and 4) according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA assay.
attributes.tag: GEO Accession
attributes.value: GSM3527317