title: GSM3595413: ChIPseq_ER_MCF7_WT_Veh_45min_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: RNA-seq: Hormone-treated cells were homogenized and total RNA was extracted using Qiagen RNAeasy kit RNA-seq: Total RNA was isolated from the experimental cell populations and primary breast tumors using Trizol (Invitrogen). Poly-A selected, stranded RNA-seq libraries were constructed using the TruSeq Stranded mRNA kit (Illumina). ChIP-seq: Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method of the paper ChIP-seq libraries were prepared using Rubicon kits.
attributes.tag: GEO Accession
attributes.value: GSM3595413