title: Comparison of RNA-sequencing  from Fresh Frozen or Formalin-fixed paraffin-embedded tissue in glioblastoma
design: RNA library was constructed using the TruSeq™ Stranded Total RNA library Prep Kit (Illumina) according to the manufacturer’s protocol with some modifications for the FFPE samples. RNA-seq reads were aligned to the human reference genome (GRCh38) using STAR (version 2.5.1b) and quantified using RSEM (version 1.1.28)