title: NextSeq 500 paired end sequencing; mRNA-seq of human cells lines derived from Papillary thyroid carcinoma (TPC1) transfected with siRNA against BHLHE40 (DEC1) or with scramble control siRNA
design: mRNA-seq of human cells lines derived from Papillary thyroid carcinoma (TPC1) transfected with siRNA against BHLHE40 (DEC1) or with scramble control siRNA
library_construction_protocol: Cell samples were collected 96 hours after transfection by trypsinization. TPC1 cells were cultured at 37째C in 5% CO2 in DMEM medium supplemented with 10% FBS Transient transfection of DEC1 or control siRNA was performed using Lipofectamine RNAi MAX at 30 nM concentration Total RNA was extracted from cell pellets using Maxwell RSC Simply RNA Cells kit (Promega) RNA-Seq libraries were prepared starting from 1 ug of total RNA, using TRUSEQ stranded RNA LT kit (Illumina)
attributes.tag: Experimental Factor: RNA interference
attributes.value: BHLHE40/DEC1 knockdown by siRNA