title: GSM2308704: SupB15_1035+Doxo-1_MNaseSeq; Homo sapiens; MNase-Seq
library_construction_protocol: Nuclear pellet from cross-linked cells was digested with micrococcal nuclease and the extent of digestion was checked on a agarose gel that showed ~70% mononucleosomes 5ng of MNase digested DNA was subjected to library construction using the NEBNext ChIP-seq Library Prep Reagent Set and Index Primer Set1 for Illumina and resolved in 1.5% agarose gel. Adaptor-ligated and index primer-containing amplified DNA corresponding to mononuclesomes was gel purified using the Qiagen Minelute Gel Extraction Kit essentially following the manufacturer’s protocol, except incubation to melt the gel was performed at 37°C for 30min. The library was then subjected to 50bp single read sequencing in an Illumina Hiseq2500 sequencer
attributes.tag: GEO Accession
attributes.value: GSM2308704