title: GSM2208761: RTKN; Homo sapiens; RNA-Seq
library_construction_protocol: HeLa cells were transfected with the plasmid containing flag-tagged RTKN by using Lipofectamine2000 (Invitrogen). After 24 h post-transfection, total RNA were isolated from transfected cells using TRIZOL reagent (Invitrogen).Poly-adenylated RNA were extracted using PolyA TruSeq RNA Sample Preparation Kit (Illumina) Total RNA from separated cell fractions was isolated with TRIZOL reagent (Invitrogen). mRNA was extracted using PolyATract mRNA Isolation Systems (Promega) Tru-seq mRNA sample preparation kit (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM2208761