title: 16srDNA of rumen fluid:low yield Holstein dairy cow
design: The samples were slowly thawed at 4C. Total DNA was extracted from the rumen fluid samples using the Stool DNA Isolation Kit (Tiangen, Beijing, China). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France), and then transferred to BGI Genomics for V4 region of the 16S rDNA gene sequencing with PE250 Miseq. The PCR primer used for 16S rDNA amplicon libraries was 515F-813R.