title: RNA-seq of human pancreatic NET sample PNET 6
design: A small piece of each snap-frozen primary tumor was pulverized using the T-prep method (Covaris 520097) on dry ice, followed by implementation of Agencourt RNAadvance (Beckman Coulter A32645) on a Biomek FXP automation workstation (Beckman Coulter) to isolate RNA. We used 150 ng RNA to prepare libraries with TruSeq Stranded Total RNA kits (Illumina RS-122-2301).