title: GSM2309922: 03-1-0149-3; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen QIAamp RNA Blood Mini kit (Germantown, MD) RNA was quantitated with a Nanodrop 800 spectrophotometer. One μg of each RNA sample was used to prepare RNAseq libraries based on the method as outlined by Zhong and colleagues [reference 17 in published manuscript] with modifications and added quality checks. Sample quantity and integrity was checked using RiboGreen analysis and an Agilent 2100 Bioanalyzer or Caliper equivalent. Each sample passing quality control (RNA mass > 1 μg and RNA Integrity Number > 6) was used to create a polyA+ stranded barcoded RNAseq library using standard protocols. Five samples were pooled for each lane for Illumina Hi-seq 2000 sequencing to generate 20–40 million mapped paired-end reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM2309922