title: GSM4409522: BTBR ob/ob wk21 1; Mus musculus; RNA-Seq
library_construction_protocol: 10X Genomics Chromium Single Cell 3' Library and Gel bead kit v2: ~6,000 singlet live cells loaded/lane, cell capture, lysis and mRNA reverse transcription occurred in-droplets. Libraries were prepared according to 10x Genomics's instructions. cDNA amplification and preparation of indexed libraries were performed using 12 and 14 cycles of PCR, respectively. Libraries were profiled using the Bioanalyzer High Sensitivity DNA kit (Agilent) and quantified using Kapa Library Quantification Kit (Kapa Biosystems).
attributes.tag: GEO Accession
attributes.value: GSM4409522