title: RNA-seq of mononuclear cells (MNCs)
design: Mononuclear cells (MNCs) were extracted from the peripheral blood mobilization of healthy volunteers and were cultured in StemSpan® SFEM (#09600, STEMCELL Technologies, America). Total RNA was isolated from MNCs using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and performed RNA-seq using HiSeq2000 at WuXi AppTec (Shanghai, China).