title: Exon-capture, Illumina HiSeq 2500: Eigenmannia_humboldtii_Atrato_EHATR1
design: The final marker set consists of 1,051 protein-coding genes after excluding loci that had either low assembly efficiency or that resulted in multiple contigs (ca. 279,012 sites). A total of 20,000 RNA baits (2x tiling) were synthesized for the marker set and used for exon capture, with samples multiplexed based on 12-plex reactions (12 samples per probe reaction). The resulting libraries were multiplexed for sequencing in three lanes of Illumina HiSeq 2500 (PE100).