title: GSM4261424: sNuc_seq_Control; Mus musculus; RNA-Seq
library_construction_protocol: mice were anesthetized, and the livers were perfused with perfusion media though the portal vein. Livers were subjected to dounce homogenization in cold swelling buffer (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 2 U/ml Superase-In). After centrifugation at 400 g, the nuclei were extracted using lysis buffer (swelling buffer with 10% glycerol and 1% Igepal). After washing once with lysis buffer, the nuclei were resuspended in chilled (4 °C) nuclei wash and resuspension buffer (1X PBS, 1.0% BSA, and 0.2 U/μl RNase Inhibitor) with nuclei concentration of 1000 nuclei/ul. 10,000 nuclei were immediately proceeded with the 10X Genomics Chromium Single-Cell 3' according to the manufacturer's instructions. The libraries from control and HepDKO liver were constructed based on the 10X Genomics Chromium Single-Cell 3' manufacturer's instructions and sequenced by Nextseq 500.
attributes.tag: GEO Accession
attributes.value: GSM4261424