title: GSM3733277: niPSC5_Rep2; Homo sapiens; Bisulfite-Seq
library_construction_protocol: DNA was purified from cell lysates and then used for library construction Whole-genome bisulfite libraries were prepared using a post-bisulfite adaptor tagging (PBAT) strategy as previously described (Smallwood, et al. (2014). Nature Methods 11, 817–820), with some modifications. Bisulfite conversion was performed on cell lysates using the Imprint Zymo direct kit. After purification, 1st strand synthesis was performed using 6N-forward oligos for 37°C for 30min. Subsequently, samples were treated with Exonuclease I for 1hr at 37°C, and DNA was purified using AMPure XP beads (Agencourt). Samples were eluted in 2nd strand synthesis mix with 6N-reverse oligo and incubated at 37°C for 90min. DNA was purified and amplified with KAPA HiFi HotStart DNA Polymerase (KAPA Biosystems). The amplified libraries were purified and assessed for quality and quantity using High-Sensitivity DNA chips on the Agilent Bioanalyzer post-bisulfite adaptor tagging (PBAT)
attributes.tag: GEO Accession
attributes.value: GSM3733277