title: GSM4262947: WT_sham_rep1; Mus musculus; OTHER
library_construction_protocol: cartilage was dissected from the knee joint and frozen in liquid nitrogen, then pulverized. DNA was extracted using trizol, followed by Qiagen DNA micro columns and eluted into water. dna was processed according to the RRHP kit (Zymo). Briefly, samples were digested with MspI, adapters were ligated and samples (except the control) were subjected to a BGT reaction. Samples were then cleaned up an subjected to a second round of MspI digestion. After the second digest, samples were cleaned up using Amgen XP beads, ligated to barcodes and subjected to 15 cycles of PCR amplification, before QC and pooling.
attributes.tag: GEO Accession
attributes.value: GSM4262947