title: WXS of Homo sapiens: adult male benign prostatic hyperplasia: Sample 8566
design: "WES was carried out on individual BPH nodules, mostly comprising mixed epithelia and stroma. DNA was extracted using the Qiagen DNeasy kit (fresh tissue) or QIAamp DNA FFPE Tissue Kit, and quantified by Nanodrop and Qubit. Whole-exome sequencing was done using the Agilent SureSelect Human All Exon V5 kit, following the modifications recommended for FFPE-derived DNA samples. Barcoded WES libraries (4 per lane) were sequenced on a HiSeq2000 to an average 60X mean bp coverage."
attributes.tag: alignment_software
attributes.value: BWA