title: 16s rRNA pyrosequencing of entile male pig feces
design: The V3-V4 regions of the 16S rRNA gene were amplified according to the 16S metagenomic sequencing library preparation protocol (Illumina, San Diego, USA) as described by (Manuel Kraler, Mahdi Ghanbari, Konrad J. Domig 2016) with modifications. gDNA was extracted from pig fecal samples.