title: GSM4262440: Donor_313C_HSCMPP_PB_420; Homo sapiens; RNA-Seq
library_construction_protocol: HSC/MPPs were purified by flow cytometry sorting from CD34+ enriched (magnetic selection) bone marrow or spleen samples as CD19- CD34+ CD38- CD45RA-. Single cell were directly sorted into lysis buffer according to the Smart-seq2 protocol published by Picelli et al., Nat. Protocol 2014. RNA libraries were prepared for sequencing using standard SmartSeq2 protocols and the Illumina Nextera XD DNA sample preparation protocol.
attributes.tag: GEO Accession
attributes.value: GSM4262440