title: Small RNA sequencing analysis of young spike by cold stress at the pistal and stamen differentiation stage in wheat
design: For the common wheat, spring frost injury frequently occurs especially when low temperatures coincide with plant from pistil and stamen differentiation stage to anther connective formation stage, which lead to the decrease of production. We sequenced four small RNA libraries of young spike at pistil and stamen differentiation stage including the cold-treated spike and the control with two biological replicates. The small RNAs (18-30 nt) were purified from 100 ug of total RNA by polyacrylamide gel electrophoresis, and ligated first to a 5’ RNA adaper and then to a 3’ RNA adaptor. After reverse transcription and polymerase chain reaction (PCR) amplification, PCR products were purified and subjected to SBS sequencing with a Illumina genome analyser.