title: 16S rRNA gene profiling using traditional PCR approach of feces sample 1
design: The 16S V3-V5 regions were amplified by PCR using modified 357F and 926R primers to allow for a two-step amplification strategy. After PCR amplification, the amplicons were purified and re-amplified to incorporate 454 sequencing-specific nucleotides and specimen-specific MIDs.