title: GSM4662140: KAM1M: cKO Male Anti-mesometrial Decidua; Mus musculus; RNA-Seq
library_construction_protocol: Freshly collected gestation day 8.5 mesometrial and anti-mesometrial decudua from littermate  control and Foxa2 conditional knockout uterus were snap frozen in liquid nitrogen. Total RNA was isolated from frozen speciment using a standard TRIzol-based protocol (Catalog 15596026, Thermo Fisher). To eliminate genomic DNA contamination, extracted RNA was treated with DNase I and purified using a RNeasy MinElute Cleanup Kit (Qiagen). The quality and concentration of RNA were determined using a Fragment Analyzer (Advanced Analytical Technologies). Libraries were prepared by Novogene using an Illumina TruSeq mRNA kit (Illumina Inc.) and sequenced (paired-end 150; 30 million read pairs) using an Illumina NovaSeq 6000.
attributes.tag: GEO Accession
attributes.value: GSM4662140