title: Illumina HiSeq 2500 sequencing; RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
design: RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
library_construction_protocol: In vitro culture protocol for human gallbladder cells. Dissociated cells from human gallbladder and cystic duct were cultured in rat mammary feeder culture using a modified culture medium as published in PMID 23562832. Adenoviral transduction of expanded/cultured human gallbladder cells and Reprogramming culture conditions of transduced human gallbladder cells Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol TruSeq RNA sample library preparation v2 as recommended by the manufacturer Illumina
attributes.tag: Experimental Factor: epithelial cell of gall bladder reprogrammed to beta cell
attributes.value: cell type
attributes.tag: Experimental Factor: Hpi1 positive
attributes.value: phenotype