design: 2-week old Arabidopsis thaiana that grew on the liquid root culture were infected with Verticillium dahliae JR2 strain. The infected samples were collected at 2 and 4 days post inoculation, respectively. These samples were equally mixed, then process to Arabidopsis AGO2 immunoprecipitation (IP), AGO2 associated RNAs were used to construct small RNA library following the miRCat small RNA cloning system (IDT, Coralville, IA).