title: GSM2309580: Quad_DM2_5; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen tissue samples were lysed using a homogenizer in Trizol. Chloroform was added and mixed, and the aqueous layer was added to isopropanol to precipitate RNA. The RNA was then cleaned up on RNeasy columns (Qiagen) with on column DNase treatment. Strand-specific libraries (using dUTP incorporation during reverse transcription, and USER treatment prior to PCR) were prepared using standard protocols. RNA was first fragmented, and then ribosomal RNA was subtracted using ribo-zero beads from Epicentre Biotechnologies. Reverse transcription was performed using random primers, followed by 2nd strand cDNA synthesis, end repair, adenylation, and ligation of adapters. Products were run on agarose, size selected, and then subjected to 14-18 cycles of PCR.
attributes.tag: GEO Accession
attributes.value: GSM2309580