title: RNA-seq of Homo sapiens:Acute_Survivor_35
design: The RNA was DNase treated usingĘAmbionĘTurbo DNase. RNAĐSeqĘlibraries were prepared from theĘDNase-treated RNA using the EpicentreĘScriptSeqĘv2 RNA-SeqĘLibrary Preparation Kit and following 10-15 cycles of amplification, libraries were purified usingĘAMPureĘXP beads. Each library was quantified using Qubit and the size distribution assessed using the Agilent 2100ĘBioanalyser. These final libraries were pooled in equimolar ratios using the Qubit and Bioanalyzer data. The quantity and quality of each pool was assessed by Bioanalyzer and subsequently by qPCR using the Illumina Library Quantification Kit from Kapa on a Roche Light Cycler LC480II according to manufacturer's instructions.Ę The template DNA was denatured according to the protocol described in the Illumina User Guide and loaded at 12ĘpMĘconcentration. To improve sequencing quality control 1%ĘPhiXĘwas spiked-in. The sequencing was carried out on the IlluminaĘHiSeqĘ2500 with version 4 chemistry generating 2 _ 125ĘbpĘpaired end reads.Ę