title: GSM3526501: DGM-10632_SAE-ALI_S57_L007 (RNA-Seq); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from nonsmoker small airway epithelium and harvested basal cells was extracted with TRIzol with subsequent RNA clean-up using the RNeasy MinElute RNA purification kit to remove residual DNA The TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) was utilized as per protocol to generate cDNA that was then purified with QIA-quick PCR purification kit (Qiagen, Valencia, CA) Samples were loaded onto an Illumina flowcell for paired-end 125 x 2 sequencing reactions using the Illumina HiSeq 2500.  HiSeq Control Software (Illumina) was used to perform image analysis, base calling and sequence analysis on HiSeq generated sequence images.
attributes.tag: GEO Accession
attributes.value: GSM3526501