title: WES of Homo sapiens: male fibroblast cell line
design: DNA was extracted from cultured fibroblasts and treated to  generate the Ion Proton AmpliSeq library. DNA was amplified in 12 separate  wells using Exome Primer Pools, AmpliSeqHiFi mix (Thermo Fisher, Waltham,  MA), and 10 amplification cycles. All 12 PCR pools were combined in one well  and subjected to primer digestion, incubated with FuPa reagent (Thermo  Fisher, Waltham, MA). Amplified exome targets were ligated with Ion Xpress P1  adapter and Ion Xpress Barcode adapters. After purification, libraries were  quantified using quantitative PCR with the Ion Library Quantification Kit  (Thermo Fisher, Waltham, MA). The prepared exome library was further used for  emulsion PCR on an Ion OneTouch System and templated Ion Sphere particles  were enriched using Ion OneTouch ES, both procedures following the  manufacturers instructions. The template-positive Ion PI Ion Sphere  particles were processed for sequencing on the Ion Proton instrument (Thermo  Fisher, Waltham, MA).