title: AccuNGS_acute_HIV
design: SuperScriptIV RT + barcoded primers; gag PCR amplicon, 40 PCR rounds with Superfi Platinum polymerase; PCR product extraction from agarose gel; Nextera XT library preparation kit with 12 PCR rounds using Superfi Platinum polymerase; library purification and size selection with AMPure XP beads; paired-end sequencing with Illumina MiSeq