title: GSM4661628: RNA sequencing from Whole mice brain RNA_C57BL/6 IRF8-/- mock #7; Mus musculus; RNA-Seq
library_construction_protocol: Mice were sacrificed 24 hour post to symptom onset followed by transcardial perfusion with ice-cold 1xPBS, brains were harvested and Total RNA was extracted using the RNeasy kit with on-column DNAse treatment, (74004; QIAGEN) according to the manufacturer's protocol. RNA quality was assessed using an Agilent TapeStation (Agilent, Palo Alto, CA, USA) and RNA concentration was quantified by Qubit 4.0 spectrophotometer. Total RNA was used as the input material for ribosomal RNA depletion and RNA sequencing library preparation. The library was prepared for sequencing using the TruSeq standard total RNA library preparation kit (Illumina, San Diego, CA, USA). The resulting cDNA libraries were quantified using the KAPA Library Quantification kit (KAPA Biosystems, Wilmington, MA, USA), and quality and size were checked using the Agilent High Sensitivity DNA Kit. Each sample was prepared using 300 ng of total RNA. The average fragment length of the cDNA libraries was 330 bp. RNA-seq libraries were sequenced using paired-end 100 bases (PE100) sequencing chemistry on Novaseq instruments following the manufacturer's protocols (Illumina). samples ran on four lane (L1-L4) and pair end sequence (R1 & R2)
attributes.tag: GEO Accession
attributes.value: GSM4661628