title: GSM3374780: RR567_P0_Tdrd12+/-;Exd1+/-_total small RNAs; Mus musculus; OTHER
library_construction_protocol: Immunoprecipitation of MILI and MIWI2 complexes from P0 (new-born pups) testes were carried out as previously described (Wenda et al., 2017). While MILI libraries were made directly from the immunoprecipitated RNAs, MIWI2-associated small RNAs (24-30nts) were purified from denaturing urea gel for library preparation. Deep sequencing libraries of P0 and P12-P14 testicular total small RNAs (19-40 nt) were prepared as described (Yang et al., 2016). Sequencing libraries were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions and sequenced on Illumina HiSeq 2000 platform (EMBL Heidelberg Gene core facility).
attributes.tag: GEO Accession
attributes.value: GSM3374780