title: GSM2310161: RNA-seq_hPGCLC d12; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq: Total RNA for each sample was extracted using Beckman Coulter's Agencourt RNAdvance Cell v2 kit following the manufacturer’s instructions and DNase treated (Ambion Turbo DNA-free). RNA-seq libraries from small sample amounts (PGCLC samples) were prepared using a modified SMART-Seq2 protocol (Picelli et al., 2014). Briefly, extracted RNA was polyA-enriched using biotinylated oligo-dT primer bound to streptavidin beads (Dynabeads) and reverse transcribed using SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified and libraries were prepared using the Nextera DNA Library preparation kit (Illumina). Libraries were indexed using Illumina Indexes and 75 bp or 50 bp single-end sequencing was performed on Illumina HiSeq 2000 instruments using TruSeq reagents (Illumina, San Diego, CA, USA), according to manufacturer’s instructions. Genomic DNA and total RNA were isolated from the samples using the Beckman Coulter's Agencourt RNAdvance Cell v2 kit following the manufacture’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2310161