title: Comparative RNA-Seq analysis of guts collected from Asian longhorned beetle larvae feeding on a nutrient-rich artificial diet and sugar maple trees
design: Four third instar Asian longhorned beetle larvae reared on a nutrient rich artificial diet and four third instar Asian longhonred beetle larvae reared in sugar maples were collected.  The larvae were surface sterilized and the guts were dissected under sterile conditions.  Total RNA was isolated using the Power Microbiome RNA Isolation Kit (MoBio) and rRNA was depleted from the sample using the Ribominus Eukaryotic Kit (Life Technologies).  Libraries were prepared using Illumina TruSeq adapters and approximately 10 million paired end reads per gut were sequenced on the Illumina HiSeq 2000.