title: GSM2210596: IMR90_Roche_1ug; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from the cell lines using standard phenol:chloroform extraction followed by ethanol precipitation. Roche NimbleGen SeqCap Epi CpGiant libraries were made using 1ug and 0.25ug of starting material, according to the company’s specifications. DNA was sonicated using a Covaris S220 sonicator to obtain products of 180-220bp. DNA was then end-repaired, A-tailed and ligated with methylated indexed-adapters to create pre-capture DNA libraries. The pre-capture libraries were bisulfite converted at 54°C for 1 hour using the Zymo EZ DNA Lightening kit. The bisulfite treated pre-capture libraries were PCR amplified with HiFi HotSart polymerase. The amplified, bisulfite converted sample libraries were then hybridized to the probe pool of fully-, partially- and un-methylated cytosines from both strands of DNA oligos at 42° C for 72 hrs. Hybridized products were purified by capture with Capture Beads and PCR amplified for 15 cycles to create the final libraries.
attributes.tag: GEO Accession
attributes.value: GSM2210596