title: GSM2308993: PE1_lncRNA; Capra hircus; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from every sample using the TRIzol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 (Agilent, CA, USA) and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number> 7.0. Approximately 10 ug of total RNA representing a specic adipose type was used to deplete ribosomal RNA according to the manuscript of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). First, ribosomal RNA was removed by using an Epicentre Ribo-zero rRNA Removal Kit (Epicentre, Madison, WI, USA). Subsequently, the high strand-specificity of the libraries was generated using the rRNA-depleted RNA of the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA), following manufacturer’s recommendations. Following purication, the RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 2×125 bp. And then we performed the paired-end sequencing on an Illumina Hiseq2500 at the (LC Sceiences, USA) following the vendor's recommended protocol. Paired-end sequencing on an Illumina Hiseq2500 (LC Sceiences, USA) following the vendor's recommended protocol.
attributes.tag: GEO Accession
attributes.value: GSM2308993