title: BGISEQ-500 sequencing; HIFI COI barcode PCR
design: Ninety-six pairs of different tags were added to both ends of a common COI barcode primer set (LCO1490 and HCO2198), with each tag containing 5 bps, allowing for ≥2 bp differences from each other. All PCR products from each plate were pooled using 1 μL per sample, resulting in 96-μL mixtures, which were sent to BGI and sequenced using BGISEQ-500. The whole PCR amplicons were construct library without fragmenting and sequenced with a strategy of 400 SE.