title: GSM3732413: ribo-amanitin_4hpf_rep2; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was isolated from zebrafish embryos harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO™ DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. The RNA was subjected to ribosomal RNA depletion with Ribo-Zero rRNA Removal Kit (Illumina) followed by library construction using NEB Next® Ultra™ RNA library Prep Kit (NEB).
attributes.tag: GEO Accession
attributes.value: GSM3732413