title: GSM4261644: scRNAseq_Celseq2_SL_Zic3_KO_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were sorted to 384-well plates which already contained primers and oil Libraries were constructed following the Celseq2 protocol from Hashimshony (2016). In brief, each cell was lysed and reverse transcribed to first strand cDNA and synthesised second strand together with ERCC-spike-in control in each well of 384-well plates. Double stranded cDNA was pooled together and purifed with Ampure XP beads. The purified cDNA pool was transribed using the IVT procedure overnight, treated with EXOI-rSAP and fragmented. RNA was purified with AMPure XP beads, reverse transcibed and amplified with indexed Illumina primers and purifed with AMPure XP beads for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4261644