title: Chuan Mai 25 (control- Replicate 1)
design: Illumina sequencing was performed using next-generation high-throughput NovaSeq 6000 RNA-Seq platform according to the manufacturers instructions (Illumina, San Diego, CA). Illumina paired-end reads (2  100 bp) were generated using RNA of Chuan Mai 25 (HT) and Ritchie (HS). NovoSeq 6000 sequencing platform generated an average of 12.38 Gb bases per sample. The raw reads were pre-processed before downstream analyses for (1) adaptor contamination, (2) reads with unknown nucleotides comprising more than 5%, (3) low quality reads with ambiguous sequence N,, and (4) very short (35 bp) sequences using SOAPnuke software (v1.5.2 available at https://github.com/BGI-flexlab/SOAPnuke).