title: GSM1670879: CTRL_nonpolyA_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: NEBNext Strand specific RNA-Seq  Sample Prep Kit (New England Biolabs, inc.) RNA-Seq: Genomic DNA and ribosomal RNA was removed with Turbo DNA-free kit and RiboMinus Eukaryote Kit (Life Technologies).  The polyA and non-polyA fractions were isolated by running RNA samples three times through the Oligo(dT) Dynabeads (Life technologies) to ensure complete separation.  The resulting RNA fractions were subjected to strand-specific library preparation using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs RNA-Seq: Subcellular fractionation was followed as described (Cell. 2012 Jul 20; 150(2): 279–290). The cell lysate was re-suspended in cold lysis buffer with 0.15% NP-40, and the sucrose buffer was used to isolate nuclei.  50% glycerol buffer and nuclei lysis buffer contains 1M Urea and 1% NP-40, were performed to isolate nucleoplasmic fraction and chromatin-bound RNA fraction.  Chromatin-bound RNA was isolated with Trizol protocol
attributes.tag: GEO Accession
attributes.value: GSM1670879