title: GSM3225991: ctrl-1hTNF-Repl2: RNA-Seq HeLa Ctr 1h TNF Repl2; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-Seq cells were harvested and total RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel).  For ChIP-Seq samples were crosslinked using Formaldehyde, Sonicated and chromatin was extracted using the p65 Antibody. DNA was purified NucleoSpin Gel and PCR Clean UP Kit with buffer NTB (Macherey-Nagel). RNA-Seq libraries were prepared from total RNA using the TruSeq Stranded total RNA LT kit (Illumina). ChIP-Seq libraries were prepared from purified ChIP DNA with the Microplex Library Preparation Kit (Diagenode)
attributes.tag: GEO Accession
attributes.value: GSM3225991