title: GSM4411453: MNP Gli3 rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Individual ChIP-seq experiments were carried out on pooled embryonic tissue collected in ice-cold PBS. Dissected tissues were immediately fixed in 1% formaldehyde/PBS for 20 minutes at room temp followed by glycine quench (125 mM). ChIP procedures were performed as previously described (Peterson et al. 2012 and Osterwalder et al. 2014).  All ChIP experiments were performed using mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich). RNA libraries were prepared for sequencing using standard Illumina sequencing protocols ChIP-seq libraries were prepared according to manufacturers' instructions and 1X75bp reads were generated on a NextSeq instrument (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4411453