title: GSM2309208: BC17 K1; Macaca fascicularis; miRNA-Seq
library_construction_protocol: Frozen kidney cortex samples (-80°C) were thawed on ice and used for total RNA extraction. Briefly, 25–30 mg of kidney cortex tissue were suspended in 700 μl QIAzol Lysis Reagent (Qiagen) and homogenized using TissueLyser for 90 seconds. Total RNA was extracted from the tissue homogenate using miRNeasy mini kit (Qiagen). Five micrograms of total RNA were used for small RNA enrichment. Small RNA enrichment  was performed using Total RNA-Seq kit v2 (Life Technologies) as per manufacturer’s recommendations.  The sequencing library was prepared from 12 ng (quantified using High Sensitivity  DNA analysis kit and 2100 Bioanalyzer) of enriched small RNAs using Total RNA-Seq kit  v2 with custom barcode primers
attributes.tag: GEO Accession
attributes.value: GSM2309208