title: GSM2552027: E13.5 Mutant Minus 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was purified using the Qiagen Rneasy Micro Kit (Qiagen, 74004) The initial amplification step for all  samples was done with the NuGEN Ovation RNA-Seq System v2. The assay was used to amplify RNA samples to create double stranded cDNA. The concentrations were measured using the Qubit dsDNA BR assay. Libraries were then created for all samples. Specifically, the Illumina protocol, the Nextera XT DNA Sample Preparation Kit, was used to create DNA library templates from the double stranded cDNA. The concentrations were measured using the Qubit dsDNA HS assay. The size of the libraries for each sample was measured using the Agilent HS DNA chip. The samples were placed in a pool. The concentration of the pool is optimized to acquire at least  15-20 million reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM2552027