title: GSM3374519: CAPH2 ChIPseq 2; Homo sapiens; ChIP-Seq
library_construction_protocol: Standard ChIP protocol was used. Briefly, nuclei were isolated using nuclearlysis buffer, followed by being lysed with SDS buffer to extract chromatin. The chromatin was sheared by Covaris sonicator to a size range of 300-700 base pairs. The chromatin samples were incubated with 6 μL antibodies overnight at 4°C with rotation, followed by incubation with Protein A/G dynabeads for 2 hours at 4°C with rotation. . The beads were washed twice with 0.1% SDS Lysis Buffer for 5 min each at 4°C, followed by washing twice with High Salt Wash Buffer for 5 min each at 4°C, followed by washing twice with Lithium Chloride Wash Buffer for 5 min each at 4°C. After that, the beads were washed with TE buffer with 0.2% Triton. Chromatin was eluted by adding elution buffer. Eluted chromatin was reverse cross-linked by adding 0.3M NaCl and incubating it overnight at 65°C, which is followed by treatment of 6 μL proteinase K. Chromatin was purified by Qiagen PCR purification kit (Qiagen, 28106), following the manufacturer's protocol. The Illumina adaptors were added to each chromatin sample for Solexa sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3374519