title: GSM2883315: Riboseq_RPF_SW480_Dox_sheIF2B5_rep2; Homo sapiens; OTHER
library_construction_protocol: Ribosomal profiling (RPF=ribosome protected fragments) and corresponding RNA-seq were performed using the TruSeq Ribo Profile for Mammalian kit from Illumina according to manufacturer's instructions. For global gene expression analysis, total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen) including on-column digestion of DNA. Poly-A+ RNA was purified from total RNA with the NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB).
attributes.tag: GEO Accession
attributes.value: GSM2883315