title: GSM3733343: Podocytes_SMPDL3b OE 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using Rneasy plus mini kit from Qiagen Preparation and sequencing of RNA libraries was carried out in the John P. Hussman Institute for Human Genomics Center for Genome Technology. Briefly, total RNA was quantified and qualified using the Agilent Bioanalyzer to have an RNA integrity score (RIN) above 7. For each of the six samples, 500 ng of total RNA was used as input for the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero to create ribosomal RNA depleted sequencing libraries. Each sample had a unique barcode to allow for multiplexing and >35 million raw reads in a 2x100 paired end sequencing run on the Illumina HiSeq 3000 were obtained
attributes.tag: GEO Accession
attributes.value: GSM3733343