title: GSM3527315: EPS-17: Mn rep2; Staphylococcus aureus subsp. aureus str. Newman; RNA-Seq
library_construction_protocol: RNA was isolated using Tri reagent and chloroform and precipitated with isopropanol. RNA was isolated using the Qiagen RNeasy Mini Kit. Precipitated RNAs were treated with DNase I (ThermoFisher) following the manufacturer's instructions. rRNA was depleted using the Ribo-Zero rRNA Removal Kit (Bacteria) (Epicentre) and paired-end cDNA libraries prepared with a TruSeq RNA Library Prep Kit v2 (Illumina). RNA sequencing was performed by the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core using the Illumina HiSeq 3000 platform (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM3527315