title: GSM3069962: MNase_YPD23_WT-A_166_40U.sgr.gz; Saccharomyces cerevisiae; MNase-Seq
library_construction_protocol: Cells were spheroplasted with zymolyase, then digested with MNase and exonuclease, treated with Proteinase K and RNase A, extracted with phenol:chloroform:IAA and ethanol precipitation. Samples were generally digested with 40 units of MNase, but a few samples also treated with 20U or 80U for control experiments. In all experiments, digested DNA was run on a low-melt agarose gel, the mononucleosomal band was gel-purified, and this DNA was sequenced. Libraries prepared with Nugen Ovation Ultralow System V2 (catalog # 0344-32).
attributes.tag: GEO Accession
attributes.value: GSM3069962