title: GSM2205845: Case2_N; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The purity of extracted RNA was measured using a NanoDrop ND- 1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE) and the concentration was assessed with Qubit 2.0 Fluorometer (Agilent Technologies, Santa Clara, CA) using an RNA HS Assay Kit. Samples with RNA Integrity Number (RIN) greater than 4 as assessed by a BioAnalyzer (Agilent) with no visible sign of genomic DNA contamination from the HS Nanochip tracings were used for total RNA library generation. Used the Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Gold (catalog #RS-122-2201), which transformed RNA into cDNA. This kit employed Ribo-Zero to remove rRNA and mitochondrial RNA prior to library preparation. The protocol consisted of random primer and stranded RNA extraction, RNA fragmentation, reverse transcription, and 100bp paired-125 end sequencing using the Illumina HiSeq 2500 system.
attributes.tag: GEO Accession
attributes.value: GSM2205845