title: WGS of C. elegans: NIL1 long-lived
design: 10 - 20,000 synchronized L1 staged worms in M9 media were processed with the Zymo genomic DNA kit following Proteinase K digestion for 3-4 hours at 55 C. Genomic DNA concentration was quantified with a Qubit fluorimetric reader using the HS kit. Genomic DNA was processed for sequencing with the Nextera DNA Library kit per manufacturer instructions. Individual samples were multiplexed, combined in equal molar ratios and sequenced on a Hi-Seq 4000 with 100bp of single end reads