title: whole genome sequence of thyroid cancer patient samples: Sample H2-WGS
design: A total amount of 1.0μg DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using Truseq Nano DNA HT Sample preparation Kit (Illumina USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The genomic DNA is randomly fragmented to a size of 350bp by Covaris cracker, then DNA fragments were end polished, A-tailed, and ligated with the full-length adapter for Illumina sequencing with further PCR amplification. At last, PCR products were purified (AMPure XP system) and libraries were analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR