title: GSM3070223: MDA-MB-231-1833shp27_cJun_ChIPSeq-rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP assay was preformed by ChIP-IT High Sensitivity kit (Active Motif; 53040). After cells were crosslinked with 1% formaldehyde, the cells were lysed and sonicated. For the immunoprecipitation, sonicated samples were incubated with anti-cJun (Cell Signaling; 9165s) and p27 (Cell Signaling; 3686s) antibodies for overnight at 4°C. After elution DNA, the quality was measured using Bioanalyzer DNA HS (Agilent Technologies). Libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit (NEB), and their quality was measured by High Sensitivity D1000 Screen Tape (Agilent Technologies). ChIP sequencing was performed on Illumina 75 cycles 400M reads.
attributes.tag: GEO Accession
attributes.value: GSM3070223