title: GSM3070912: WAGO-4-associated small RNAs after lin-15b RNAi; Caenorhabditis elegans; miRNA-Seq
library_construction_protocol: Small RNAs were cloned and deep sequenced using an Illumina platform, according to the manufacturer's instructions, by the Beijing Genomics Institute (BGI Shenzhen). sRNAs ranging from 17 to 30 nt were gel-purified and ligated to a 3' adaptor (5'-pUCGUAUGCCGUCUUCUGCUUGidT-3'; p, phosphate; idT, inverted deoxythymidine) and a 5' adaptor (5'-GUUCAGAGUUCUACAGUCCGACGAUC-3'). The ligation products were gel-purified, reverse transcribed, and amplified using Illumina's sRNA primer set (5'-CAAGCAGAAGACG GCATACGA-3'; 5'-AATGATACGGCGACCACCGA-3'). The samples were sequenced using an Illumina Hiseq platform.
attributes.tag: GEO Accession
attributes.value: GSM3070912