title: GSM3466179: NipblHET primary dissociated cortical neuron untreated Rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was obtained in parallel from dissociated cortical neurons at DIV10 without stimulation (baseline); after overnight treatment with TTX and D-AP5 (TTX); and after overnight treatment with TTX and D-AP5 and depolarization with KCl for 1h (KCl1h) or 6h (KCl6h). RNA was extracted with QIAshredder and RNeasy mini kit (Qiagen). RNA-seq libraries were prepared from 600 ng of total RNA (RNA integrity number (RIN) >8.0) with TruSeq Stranded Total RNA Human/Mouse/Rat kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3466179