title: GSM3676139: dKO+EoVP16_cells_d5_rep1 (RNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: RNA from differentiated embryoid bodies was extracted with RNeasy Mini Kit (Qiagen) with on-column DNAse digestion as recommended by the manufacturer. Library preparation was carried out using 0.5 μg of RNA with NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs). RNA from E7.5 epiblasts was isolated using RNeasy Micro kit (Qiagen). Libraries were prepared from 10 ng of RNA with the Ovation SoLo RNA-seq Systems kit (NuGEN).
attributes.tag: GEO Accession
attributes.value: GSM3676139