title: Illumina HiSeq 4000 sequencing; Single cell RNA-seq of mouse skin cells
design: Single cell RNA-seq of mouse skin cells
library_construction_protocol: Skin samples from two 8-week old female C57BL/6 mice were processed, first by mechanical processing, followed by 2 h incubation with 0.5% collagenase B (Roche; 11088815001). Skin samples from two 8-week old female C57BL/6 mice were processed, first by mechanical processing, followed by 2 h incubation with 0.5% collagenase B (Roche; 11088815001). Cells were then counted and loaded on the 10x Chromium machine. Libraries were prepared following the Chromium Single Cell 3′ v2 Reagent Kit Manual54.
attributes.tag: Experimental Factor: individual
attributes.value: 3421STDY7639029