title: IAA-72
design: Auxin was recognized as a secondary dormancy phytohormone, controlling seed dormancy and germination. To understand how auxin affected seed dormancy and germination, the transcription expression profilings of seed tissues from AUX-treated water-treated and control seeds at both dormancy and germinated stages were obtained through RNA sequencing. We collected seed samples at the dormancy stage and germination stage from AUX-treated water-treated and control seeds respectively. Seed tissues were frozen in liquid nitrogen as soon as possible, and RNA was isolated from mixed leaf samples using the RNeasy Plant Mini kit.