title: GSM3466823: SUB-ENDOSTEUM Sample S14; Mus musculus; RNA-Seq
library_construction_protocol: Mouse femurs were fixed in 4% PFA and cryosectioned at 12 um thickness. Sections were stained using fluorescently labelled antibodies against Sca-1 and EMCN, and Hoechst 33342 was used as a counterstain. Samples were imaged and collected using the Zeiss PALM MicroBeam Axio Observer Z1 (Zeiss). Areas of 14500 µm2 on average, corresponding to around 250-300 cells, were isolated in proteinase K solution. Reverse crosslinked RNA was extracted with 100µl TRI Reagent (Sigma-Aldrich) following manufacturer instructions and cDNA synthesized following the SMARTER stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio, Japan) protocol. SMARTER stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio, Japan)
attributes.tag: GEO Accession
attributes.value: GSM3466823