title: Gene expression of liver sinusoidal endothelial cells after -melittin-NP treatment
design: the total RNA from LSECs was extracted using TRIzol (Invitrogen, Carlsbad, CA). Total RNA (2 g) was used for thestranded mRNA sequencing library preparation using the VAHTS mRNA-seq v2 Library Prep Kit for Illumina (Vazyme biotech Co., Nanjing, China), following the manufacturers instructions. ThePCR products that were 200500 bps in length were purified, quantified and finally sequenced on aHiSeq X10 sequencer (Illumina).