title: GSM2309139: cKI_Mx_pos_827 [Bisulfite-Seq]; Mus musculus; Bisulfite-Seq
library_construction_protocol: bone marrow was extracted from femurs, tibiae and iliac crests. After red blood cell lysis and magnetic lineage depletion cells were FACS-sorted to isolate Lin- Sca1+ cKit+ (LSK) cells. Total RNA was extracted using AllPrep Kit (Qiagen) Genomic DNA was purified from murine cells, digested with MspI restriction enzyme, subjected to end repair and adenylation followed by ligation to cytosine methylated paired-end Illumina adaptors. Fragments of 150-400 bp were isolated by size selection and subjected to bisulfite conversion and PCR amplification using paired-end Illumina primers.  Libraries were sequenced using an Illumina HiSeq2500 per manufacturer’s recommended protocol for 50bp single end read reads
attributes.tag: GEO Accession
attributes.value: GSM2309139