title: RNA-Seq of wildype BY4741 Saccharomyces cerevisiae strain: YPD, replicate 1
design: Total RNA isolated from the specified strains was ribodepleted using the Ribo-ZeroTM Magnetic Gold Kit (Yeast) (Epicenter, cat# MRZY1324) according to manufacturers instructions. Equal amounts of the depleted RNA was then used for strand specific library construction utilizing the TruSeq Stranded Total RNA LT (Illumina, RS-122-2302). The libraries from 8 samples were pooled together and run on a single lane of HiSeq2500 machine for sequencing 50bp single end reads.