title: GSM3223782: Input_BAF60cKO_CM_R1; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP was performed following  O'Geen et al., 2011, Wamstad et al., 2012 with modifications. Briefly, 50 million cells were crosslinked with disuccinimidyl gulatarate  (45 min) followed by  formaldehyde (15min) and quenched, chromatin was digested with MNase to mono and di-nucleosomes, BRG1 was immuniprecipitated using anti-BRG1 antibody (ab110641, lot no. )and washed extensively and DNA eluted followed by reverese crosslinking, proteinase  and RNAse treatment and ampure purification of DNA (Serandour et al., 2013). Libraries were prepared mannually.  Briefly, DNA was end-repaired using the End-IP repair kit (Epicenter, # ER81050). DNA were A-tailed with Klenow fragment (3' to5' exo minus) and dATP.  Barcoded Illumina  adapters were ligated and DNA was PCR amplified  with Illumina primers for 14 cycles. 300- 500bp library fragments were size selected in an agarose gel and purified.   Libraries were quantified by Qubit and analyzed in a Bioanalyser. Libraries were diluted and sequenced in a NEBNextSeq sequencing system following manufacturer's instructutions.
attributes.tag: GEO Accession
attributes.value: GSM3223782