title: GSM3215283: MEL06_T0_1c TAGCGCTC-GCGTAAGA; Homo sapiens; OTHER
library_construction_protocol: Mice with tumors reaching 1000 mm³ were started on the BRAF-MEK combination via daily oral gavage (Instech Laboratories). BRAF inhibitor dabrafenib (Tafinlar capsules) and MEK inhibitor trametinib (MCE) were dissolved in DMSO at a concentration of 30 and 0.3 mg/mL respectively, aliquoted and stored at -80°C. Each day 900 μL PBS (Sigma) was added to a new aliquot. Mice were treated with a capped dose of 600 – 6 μg dabrafenib – trametinib respectively in 200 μL total volume. This tumor was minced followed by dissociation using collagenase I & IV (Sigma) and trypsin (Life Technologies). Cells were resuspended in serum-free DMEM/F12 medium (Thermo Fisher Scientific) and 250 000 cells were injected in the interscapular fat pad of 8 – 16 week old female NMRI nude mice (Taconic).
attributes.tag: GEO Accession
attributes.value: GSM3215283