alias: GSM2890297
title: GSM2890297: small RNA in sperm; Gallus gallus; ncRNA-Seq
library_construction_protocol: Total RNA from sperm sample were extracted using Phenol Water (Solarbio, Beijing, China) according to the manufacturer's procedure. The quantity and purity of total RNA were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip TM Kit (Agilent, CA, USA) with RIN number >7.0. Equal quantities of the RNA extracted from the sperm of five cockerels were pooled into one sample. Approximately 1 μg of total RNAs were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep TM Kits (Illumina, San Diego, USA), and then we performed the single-end sequencing (1*50bp) on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) following the recommended protocol (Chen et al. 2015).
identifiers.namespace: GEO
identifiers.id: GSM2890297