alias: GSM3472987
title: GSM3472987: MZ_RT1; Maylandia zebra; RNA-Seq
library_construction_protocol: Animals were sacrificed and immediately dissected for RNA extraction. A ribbon approximately 1mm x 10mm of epithelium was removed labial to the outer row of teeth from the dentary of experimental animals using a #12 scalpel blade. The extra-osseous soft tissue was removed from the entire jaw to reduce the risk of TB containing epithelium carryover. The bone was then shaved down using a scalpel to expose the bony crypts and intraosseous RT were extracted with fine forceps. Extracted tissue was quickly placed in RNAlater RNA Stabilization Reagent (Qiagen). Tissues were frozen in liquid nitrogen, homogenized using a mortar and pestle and placed in trizol. Following standard chloroform extraction RNeasy mini columns (Qiagen) were utilized to purify RNA for storage at -80ᴼC. Total RNA was quantified using Qubit (Molecular probes) and quality analyzed using the Agilent 2100 Bioanalyzer System for RNA library preparation. RNA input was normalized to 1µg and libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina- Kit A). Libraries were again quantified, quality assessed, and normalized for sequencing on the HiSeq 2500 Illumina Sequencing System.
identifiers.namespace: GEO
identifiers.id: GSM3472987