alias: GSM2319662
title: GSM2319662: sox11crush.B; Mus musculus; RNA-Seq
library_construction_protocol: After sacrifice, retinas were dissected in serum-free culture media and kept cool on ice. 3 retinas were pooled for each treatment condition for each replicate. Then, retinas were dissociated with papain, quenched with trypsin inhibitor, triturated, and stained with anti-Thy1-PE-Cy7 antibody and SYTOX live-dead cell stain. Cells were then sorted directly into Arcturus PicoPure extraction buffer, lysed immediately, and RNA was extracted according to manufacturer's instructions. Nugen Ovation RNA Ultra Low Input (500 pg) +TruSeq Nano
identifiers.namespace: GEO
identifiers.id: GSM2319662