alias: GSM4416498
title: GSM4416498: PH-T3-16-WT; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using STAT-60 (AMS biotechnology, CS-111)/chloroform (200 uL; Sigma, Cat 650471)/isopropanol (500 uL; Sigma, cat 33539)/75% ethanol (1ml) according to previously published protocol (PMID: 26679101). Samples were stored in aliquot at – 80 °C prior to use. All reagents, plastic ware, and supplies used were sterile, nuclease free, and of molecular biology grade. RNA purity (A260/A280>1.80) and concentration were determined using nanodrop spectrophotometer (Thermo Fisher scientific, Delaware USA). RNA integrity was studied using the 2100 Bioanalyzer System (Agilent) and RNA 6000 Nano Kit. An RNA Integrity Number (RIN) of 8 was considered the lowest cut-off for RNA Sequencing. Sequencing libraries were constructed using Illumina mRNA library prep kit.
identifiers.namespace: GEO
identifiers.id: GSM4416498