alias: GSM1678001
title: GSM1678001: Chicken-DT40-MSH3; Gallus gallus; OTHER
library_construction_protocol: Nuclei were extracted from tissues and cell lines. All the reagents used were pre-chilled and the entire procedure was performed on ice. Tissues and cells were homogenized by douncing 50 times in 10 mL of Nuclear Extraction Buffer. After homogenization, samples were layered on 18 mL of Sucrose Cushion in 40 mL ultracentrifuge tubes (Beckman, 25 × 89 mm, 344058) and spun at 30,000 x g for 45 min at 4°C (Beckman, L8-70M, SW28 rotor). Supernatant, including debris, was removed, and 200 μl of chilled 1 × PBS was added to the tube. After 20 min incubation on ice, the nuclei were dissociated by pipetting, and recovered by centrifugation at 500 x g for 5 min.
identifiers.namespace: GEO
identifiers.id: GSM1678001