alias: GSM2889541
title: GSM2889541: D23580_inSPI2_TEX; Salmonella enterica subsp. enterica serovar Typhimurium str. D23580; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol, and treated with DNase I as described previously (Kroger et al., 2012 PNAS). RNA integrity was inspected visually with the Bioanalyzer (Agilent Technologies). Contaminating DNA was removed using DNase I (Ambion) and RNA samples were not ribo-depleted prior to cDNA library construction. cDNA library construction, TEX-treatment for dRNA-seq (ESP, InSPI2 and pooled sample) and RNA-seq with the Illumina HiSeq platform was carried out by Vertis Biotechnologie, Germany. All protocols were identical to those used previously (Kroger et al., 2012; Kroger et al., 2013).
identifiers.namespace: GEO
identifiers.id: GSM2889541