alias: E-MTAB-7215:Spore 3_p
title: NextSeq 500 paired end sequencing; RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum
design: RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum
library_construction_protocol: To isolate spores, 24 h fruiting bodies were collected, dissociated and sieved through 50 µm nylon mesh as described above. The flow-through containing spores was passed next through 35 µm nylon mesh, and incubated with 0.1% Triton X-100 for 5 minutes to remove amoeboid cells. RNeasy kit (QIAGEN) was used for extraction Libraries were prepared with Illumina Truseq stranded mRNA library preparation kit.
identifiers.namespace: School of Life Sciences, University of Dundee
identifiers.id: E-MTAB-7215:Spore 3_p