alias: GSM3681474
title: GSM3681474: RNAseq R-2HG Treatment at 24h (replicate 2); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from HUVEC cells using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with a LabChip Gx Touch 24 (Perkin Elmer). 4 μg of total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in minimum of 32M reads per library with 1x75bp single end setup.
identifiers.namespace: GEO
identifiers.id: GSM3681474