alias: GSM4272425
title: GSM4272425: A375-Control-R1; Homo sapiens; RNA-Seq
library_construction_protocol: For Control, Top-5 and Bottom-5, RNA was extracted using Qiagen RNAeasy plus mini kit. RNA libraries were prepared using NebNext Ultra II RNA library prep Kit. For Top20-5, Bottom20-5, Top-12 and Bottom-12, RNA was extracted using the Qiagen RNAeasy plus mini kit as described above. Total RNA was then sent for library preparation and sequencing at GeneWiz. RNA libraries were prepared using NebNext Ultra II RNA library prep Kit (Cat. No. E7770G). After rRNA depletion, RNA was fragmented (~200 nt) and primed following first and second strand cDNA synthesis. The double stranded cDNA was then purified using AmpureXP beads at a 1.8X concentration. After purification, cDNA libraries were end prepped, ligated adapter and PCR amplified for 7 cycles to enrichment for adaptor ligated DNA using NebNext Multiplex Oligos for illumine (Cat. No. E7335G). cDNA librariers were then purified using AmpureXP beads at a 0.9X concentration and their quality was checked using Agilent Bioanalyzer high sensitivity DNA kit. cDNA samples were then sent to Oklahoma Medical Research Facility (OMRF) for high throughput sequencing.
identifiers.namespace: GEO
identifiers.id: GSM4272425