alias: GSM2809115
title: GSM2809115: gun1-1+NF1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA-extraction protocol using citric acid described by Onate-Sanchez and Vicente-Carbajosa (2008). Total RNA of 5d old seedlings was extracted. After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. For prokaryotic samples, rRNA is removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
identifiers.namespace: GEO
identifiers.id: GSM2809115