alias: CHWR37
title: Chokwe: 16S rRNA gene metabarcode at least five years fallow field Rep 1
design: The hypervariable V4 region of 16S rRNA library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage amplicon PCR step involving 0.2 M concentrations of primers 515F (forward) and 806R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter and Trimmomatic software
identifiers.namespace: SUB4964592
identifiers.id: CHWR37