alias: GSM2311138
title: GSM2311138: adipose_replicate_15 [intravenous LPS]; Homo sapiens; RNA-Seq
library_construction_protocol: RNA samples were extracted using RNeasy Lipid Tissue total RNA mini kit  (Qiagen, Valencia, CA). Extracted RNA samples underwent quality control (QC) assessment using the Agilent Bioanalyzer (Agilent, Santa Clara, CA) With a minimum of 1 µg input RNA, we generated first-strand cDNA using random hexamer-primed reverse transcription, followed by second-strand cDNA synthesis using RNase H and DNA polymerase, and ligation of sequencing adapters using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA). Fragments of ~350 bp were selected by gel electrophoresis, followed by 15 cycles of PCR amplification. The prepared  libraries were then sequenced using Illumina's HiSeq 2000 with two lanes per sample which generated 2×101 bp or 2x100 bp paired-end reads in ~200 to 500 million unfiltered reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM2311138