alias: GSM2735767
title: GSM2735767: 3xFLAG-CsrA 5; Escherichia coli str. K-12 substr. MG1655; RIP-Seq
library_construction_protocol: Samples were thawed, lysed with sonication over ice, and cleared with centrifugation. CsrA-RNA complexes were immunoprecipated with M2 anti-FLAG mouse antibody conjugated paramagnetic particles (Sigma) for 4 hours at 4C. Complexes were washed, 5'-end labeled with 32P, and eluted from the particles by heating in non-reducing PAGE sample buffer. Protein-RNA complexes were seperated with SDS-PAGE and transferred to nitrocellulose. RNA was extracted from the nitrocellulose by treatment with proteinase K,  phenol chloroform extraction, and ethanol precipitation. NEB small RNA library prep kit (Catalog #: E7300S)
identifiers.namespace: GEO
identifiers.id: GSM2735767