alias: GSM2212036
title: GSM2212036: DMSO-3hr (PRO-seq); Homo sapiens; OTHER
library_construction_protocol: Nuclei isolation by douncing in isotonic buffer with 0.1% Triton X-100 Nuclear run-on assays were performed with ATP, UTP, GTP, and biotin-11-CTP or with all 4 biotin-11-NTPs. Nuclei were lyzed with TRIzol, nuclear RNA was extracted and fragmented, and biotinylated RNA was purified with strepavidin magnetic beads, which was followed by 3' adaptor ligation, 5' repair, 5' adaptor ligation, reverse transcription, and PCR full-scale amplification.
identifiers.namespace: GEO
identifiers.id: GSM2212036