alias: steffi_pgc_bs_experiment_0:0:5:0
title: E16.5m BS-Seq 1 experiment
design: BS-Seq DNA Methylation Profiling
library_construction_protocol: The amount of input material for the BS-Seq libraries was between 5ng and 50ng genomic DNA. The input DNA was sonicated and end-repair and A-tailing were performed using the NEB Next kit according to the manufacturer's instructions. Illumina's Early Access Methylation Adaptor Oligo Kit was used for the adapter ligation. The adapter-ligated DNA was treated with sodium-bisulfite using the Imprint DNA Modification Kit from Sigma-Aldrich according to the manufacturer's instructions for the two-step protocol. Bisulfite-treated DNA was amplified using PfuTurbo Cx Hotstart DNA Polymerase from Agilent Technologies with 14-18 cycles depending on the input amount. Size selection was performed by gel extraction for DNA fragments between 200bp and 250bp.
identifiers.namespace: BI-UK
identifiers.id: steffi_pgc_bs_experiment_0:0:5:0