alias: GSM3530284
title: GSM3530284: FANTOM-19-39867266; Homo sapiens; RNA-Seq
library_construction_protocol: Sample preparation: Histologically normal margins of healthy colonic resections were processed, digested and stained for FACS analysis as previously described. CD45+ CD64+ HLA-DR+ CD206+ and CD45+ CD64+ HLA-DR+ CD206- cells were sorted using a FACSAria II/III. Cells were sorted into 300 μl RLT buffer (Qiagen) and stored at -80°C prior to RNA extraction using a MicroRNA kit (Qiagen). Sequencing libraries were prepared using the SMARTer Stranded Total RNA-Seq kit-Pico Input Mammalian (Clontech), starting with 1ng of RNA and following the manufactures protocol with 15 cycles of PCR.
identifiers.namespace: GEO
identifiers.id: GSM3530284