alias: GSM4663168
title: GSM4663168: 2-OVLT-single_cell_RNASeq; Mus musculus; RNA-Seq
library_construction_protocol: Animals were anaesthetized with isoflurane. Brains were extracted and dissected in NMDG-ACSF. Tissue was digested with papain in trehalose-HEPES-ACSFand triturated to generate single cell suspensions. For stimulus to cell-type mapping experiments, actinomycin-D was included in NMDG-ACSF and trehalose-HEPES-ACSF to block artificial induction of immediate early genes. Sequencing libraries were constructed following 10x Genomics 3' v2 (Samples 1, 2) or v3 )Samples 3-10) standard protocols. 3' Single-cell RNA-Seq
identifiers.namespace: GEO
identifiers.id: GSM4663168