alias: GSM3678166
title: GSM3678166: C_2; Glycyrrhiza uralensis; RNA-Seq
library_construction_protocol: Flash frozen on dry ice, and RNA was harvested using Trizol reagent protocol. Total RNA was extracted and detected, mRNA was separated and purified from total RNA using poly-Toligo attached magnetic beads, and then broken into small fragments using divalent cations under conditions of elevated temperature. First-strand cDNA was synthesized using a random hexamer primer and M-MuLV Reverse Transcriptase (NEB), followed by second strand cDNA synthesis using buffer, deoxyribonucleotide triphosphates (dNTPs), DNA polymerase I, and Ribonuclease H (RNase H). Finally, PCR amplification was performed and the PCR product was purified with AMPure XP beads to obtain the final library. After the library quality was assessed on the Agilent Bioanalyzer 2100 System.
identifiers.namespace: GEO
identifiers.id: GSM3678166