alias: GSM2891355
title: GSM2891355: Whole genome bisulphite sequencing HSC CFP Dim Pool 1; Mus musculus; Bisulfite-Seq
library_construction_protocol:  HSCs were lysed in 10 ul M-digestion buffer and 1 ul proteinase K both from the EZ DNA Methylation-Direct Kit (Zymo Research), and incubated for 20 min at 50 degrees. Bisulfite conversion of DNA and library preparation were performed using the Pico Methyl-Seq Library Prep Kit (Zymo Research) and sequenced on a NextSeq500 (Illumina)  Libraries from two pooled replicates of RA-CFP-dim and –bright HSCs were sequenced on a NextSeq500
identifiers.namespace: GEO
identifiers.id: GSM2891355