alias: GSM4414334
title: GSM4414334: G2_2i_SmartSeq2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from 2iLif ESCs and L-EPSCs reprogramming intermediates at indicated days using TRIzol reagent according to manufacturer's protocol. 4 µg of total RNA was used to generate libraries using of stranded poly(A) mRNA-Seq library with the KAPA stranded mRNA Library prep kit (KAPA Biosystems, KK8421). Libraries concentration was quantified with Qubit dsDNA HS (High Sensitivity) Assay Kit (Invitrogen, Q32854), and the final pool was generated by combining individual libraries in equimolar ratio. Libraries were sequenced on an Illumina HiSeq 4000 instrument (Illumina) with 51 bp reads and read depth ranging ~33 mln reads. Single-cells from stem cells were sorted by FACS into 384 well plates containing lysis and RT, whereas embryo cells were manually picked and directly dispensed into lysis buffer containing RT. The current study generated cDNA libraries using the Smart-seq2 protocol.
identifiers.namespace: GEO
identifiers.id: GSM4414334