alias: GSM2466698
title: GSM2466698: trm4b-4 2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: 9-day-old Arabidopsis wild-type and trm4b-4 seedlings were harvested for total RNA extraction. The amount and quality of RNA were tested by Qubit RNA Assay Kit (Life Technologies), gel electrophoresis and Agilent Bioanalyzer 2100 system. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) was used to construct the libraries according to the manufacturer’s instructions, and sequencing was performed on Illumina Hiseq 4000 platform.
identifiers.namespace: GEO
identifiers.id: GSM2466698