alias: GSM2735055
title: GSM2735055: AOMDSSCurcumin_1 [RNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2735055