alias: GSM2049380
title: GSM2049380: RI08; smallRNA-Seq; Oryza sativa Indica Group; ncRNA-Seq
library_construction_protocol: Small RNA libraries were constructed using previously described methods. Briefly, 10–30 nt small RNAs were purified using a 15 % denaturing polyacrylamide gel , then ligated with 5′ and 3′ adapters, then converted to cDNA by RT-PCR using Superscript II reverse transcriptase (Invitrogen), small RNAs were amplified by PCR, gel-purified then submitted for deep sequencing using the Illumina Genome analyzer (Illumina) at the Beijing Genomics Institute (BGI, Shenzhen, China).
identifiers.namespace: GEO
identifiers.id: GSM2049380