alias: GSM1673660
title: GSM1673660: MEL_4H8_ChIAPET_D5R1; Mus musculus; OTHER
library_construction_protocol: Biological replicates of RNA Polymerase II ChIA-PET libraries for uninduced (MEL) and induced (MEL+DMSO) MEL were essentially constructed as previously described (Li et al. 2012) with additional changes to volume used in circularization (Heidari et al. 2014). The starting material was from 100-200 million cells and the ChIPs were performed using mouse anti-RNA polymerase II 4H8 antibody (catalog number sc-47701X, Santa Cruz Biotechnology, CA) and Dynabeads M-280 sheep anti-mouse beads (Life Technologies). MEL total RNA was extract by RNeasy Kit (QIAGEN) and the quality was checked by RIN.
identifiers.namespace: GEO
identifiers.id: GSM1673660