alias: GSM1675784
title: GSM1675784: Tet2 knock-down oxBS-seq replicate 3; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA were extracted using Qiagen DNeasy Blood and tissue kit by following manufacture’s instruction. Purified genomic DNA were equally split into two. One part were treated with KRuO4 following the protocol from PMID: 24008380. Then both genomic DNA were parallelly treated with sodium bisulfite using MethylCode bisulfite conversion kit (Life Technologies). Targeted regions were amplified using PyroMark PCR kit and cleaned up with MinElute reaction cleanup kit (Qiagen).  Amplified fragments were multiplexed using Illumina TrueSeq DNA library preparation kit. Pooled libraries were sequenced using MiSeq sequencing instrument (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM1675784