alias: GSM3681020
title: GSM3681020: Replicate 2 RR-ChIP seq D210N; Homo sapiens; OTHER
library_construction_protocol: Lysates were clarified from sonicated nuclei and 1) RNA:DNA hybrids were isolated with S9.6 antibody for RDIP-seq 2) RNA:DNA hybrids were isolated with GFP antibody for RR-ChIP-seq For the RDIP-seq and RR-ChIP-seq, the RNA component of the R-loop was used to prepare the library using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, (E7760S) according to manufacturer's guidelines. For the ChrRNA-seq and ChrCAP-seq, chromatin associated RNA was used for library prepration as for RDIP-seq.
identifiers.namespace: GEO
identifiers.id: GSM3681020