alias: GSM4546369
title: GSM4546369: Vav-Ezh2-WT17; Mus musculus; ATAC-seq
library_construction_protocol: 500 erythro-myeloid progenitors were sorted into a 0.5-ml DNA LoBind tube (Eppendorf #022431005) containing 23.75 μl of transposition mix (8.25 μl PBS, 2.5 μl water, 12.5 μl 2× TD Buffer, 0.25 μl 1% digitonin and 0.25 μl 10% Tween-20). 1.25 μl Tn5 transposase was added to the tube and incubated at 37 °C for 30 min in a thermomixer with shaking at 300 r.p.m. Reactions were cleaned up with Qiagen MinElute Kit. The transposed DNA was pre-amplified/indexed with 14 cycles of PCR with NEBNext High-Fidelity 2x PCR master mix. Samples were sequenced using the Illumina NextSeq 500/550 platform.
identifiers.namespace: GEO
identifiers.id: GSM4546369