alias: GSM3467396
title: GSM3467396: GO3_sc121; Mus musculus; Bisulfite-Seq
library_construction_protocol: All the animal procedures were carried out according to the ethical guidelines of Laboratory Animal Center of the Sichuan University. None-growing oocytes (NGO) with diameters less than 20μm were collected from 3- to 4-day-old female ICR mice. Growing oocytes (GO) and fully-growing oocytes (FGO) were collected from 3- to 4-week-old female ICR mice, the diameters of oocytes for each stage were: GO1 (30μm< diameters <45μm), GO2 (50μm< diameters <55μm), GO3 (60μm< diameters <65μm), FGO (diameters >70μm). Excessive washes were performed to remove the contamination of somatic cells from oocytes. Individual oocyte was then picked for the downstream single-cell experiments. Firstly, individual oocyte was in vitro methylated by GpC methylase (New England Biolabs), digested by protease and bisulfite converted26. Then, the single-cell genomic DNA was randomly primed and dC tailed. The oligos with anchor sequences were then introduced to fragmented genomic DNA and PCR amplified. Finally, libraries were checked, pooled together and sequenced with paired-end 150-bp reads on Illumina HiSeq 4000 platform (Novogene).
identifiers.namespace: GEO
identifiers.id: GSM3467396