alias: GSM2891408
title: GSM2891408: Tumor_Wisedale_2; Sarcophilus harrisii; Bisulfite-Seq
library_construction_protocol: DNA methylation profiling by RRBS was performed using 100 ng of genomic DNA isolated from RNA-later preserved tissue samples through the Allprep DNA/RNA Mini kit (QIAGEN). DNA was digested using the restriction enzymes MspI and TaqI in combination in order to increase genome-wide coverage. Restriction enzyme digestion was followed by fragment end repair, A-tailing, and adapter ligation. The amount of effective library was determined by qPCR, and samples were multiplexed in pools of 13 with similar qPCR Ct values. The pools were then subjected to bisulfite conversion followed by library enrichment by PCR. Enrichment cycles were determined using qPCR and ranged from 9 to 13 (median: 11). After confirming adequate fragment size distributions on Bioanalyzer High Sensitivity DNA chips (Agilent), libraries were sequenced on Illumina HiSeq 3000/4000 machines in a 50 base pair single-read setup.
identifiers.namespace: GEO
identifiers.id: GSM2891408