alias: E-MTAB-7883:Sample 27_s
title: Illumina MiSeq sequencing; Effects of HIV infection and opioids on gut microbiota
design: Effects of HIV infection and opioids on gut microbiota
library_construction_protocol: Fecal content was collected from gut region encompassing distal cecum and approximately one inch of the colon and frozen on dry ice.  The fecal matter was lysed using glass beads in MagnaLyser tissue disruptor (Roche) and total DNA isolated using Power-soil/fecal DNA isolation kit (Mo-Bio) as per manufacturer's specifications. All samples was quantified via the Qubit® Quant-iT dsDNA Broad- Range Kit (Invitrogen, Life Technologies, Grand Island, NY) to ensure that they met minimum concentration and mass of DNA and submitted to University of Minnesota Genomic Center for 16s rRNA sequencing . Using QIIME, sequences were quality filtered and demultiplexed using exact matches to the supplied DNA barcodes.
identifiers.namespace: Surgery/Surgical Oncology, University of Miami
identifiers.id: E-MTAB-7883:Sample 27_s