alias: GSM2464914
title: GSM2464914: 1834-tumor-input; Homo sapiens; RIP-Seq
library_construction_protocol: Tissues were homogenized in Trizol reagent with a Tissure-Tearor. Total RNA were extracted using manufacturer’s instructions.Poly-adenylated RNA was enriched by using FastTrack MAG Maxi mRNA isolation kit (Invitrogen). RNA fragmentation, m6A-seq, and library preparation were performed as in Dominissini et al 2013 Nature Protocols. Cell line total RNA was isolated from stable cells with TRIZOL reagent. Poly-adenylated RNA was enriched by using FastTrack MAG Maxi mRNA isolation kit (Invitrogen). RNA fragmentation, m6A-seq, and library preparation were performed as in Dominissini et al 2013 Nature Protocols. TruSeq Small RNA Sample Preparation Kit (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM2464914