alias: E-MTAB-7446:T2007_s
title: NextSeq 500 sequencing; Comprehensive investigation of miRNome identifies novel candidate miRNA-mRNA interactions implicated in T-cell acute lymphoblastic leukemia
design: Comprehensive investigation of miRNome identifies novel candidate miRNA-mRNA interactions implicated in T-cell acute lymphoblastic leukemia
library_construction_protocol: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research).  Libraries were generated with NEBNext® Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, MA, USA).
identifiers.namespace: Institute of Human Genetics Polish Academy of Sciences
identifiers.id: E-MTAB-7446:T2007_s