alias: GSM3304509
title: GSM3304509: POOL1__pNoCMVMPRA1__HeLa__rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from transiently transfected K562, HeLa, and HepG2 was precipitated by phenol-chloroform extraction according to standard protocols. DNase treatment (Worthington) was followed by cDNA synthesis with SuperScript III First-Strand Synthesis System (Invitrogen). cDNA was subject to library amplification and libraries were cleaned up with AMPure beads (0.6x, 1.6x, 1.0x). plasmid sequencing and RNA-seq
identifiers.namespace: GEO
identifiers.id: GSM3304509