alias: GSM2889684
title: GSM2889684: SLX-11179_L-cells_35; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was prepared from 3 male mice, aged 20-21 weeks. Intestinal pieces were stripped of the outer muscle layers. Tissue was chopped into 1–2 mm pieces and digested to single cells with 1 mg/ml collagenase in calcium-free Hanks Buffered Salt Solution (HBSS). Single cell suspensions were separated by FACS using an InfluxCell Sorter (BD Bioscience, USA). Cells were directly sorted into individual wells of a 96-well PCR plate containing 2.3 µl of 0.2% Triton-X100 (Sigma-Aldrich, Cat. no. 93443) and 2.3 U of SUPERase-In RNase Inhibitor (Ambion, Cat no. AM2694). cDNA was obtained and amplified following the described SMARTSEQ2 protocol (Picelli et al., 2014). Libraries were prepared for sequencing from 125 ng of cDNA using the Illumina Nextera XT DNA preparation kit. Pooled libraries were run on the Illumina Hi-Seq 2500. Libraries were prepared for sequencing from 125 ng of cDNA using the Illumina Nextera XT DNA preparation kit.
identifiers.namespace: GEO
identifiers.id: GSM2889684