alias: GSM4413771
title: GSM4413771: KO 3-3 RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Neurons were washed 1x with ice-cold PBS and collected with 1 mL per 6-well TRIzol (Life Technologies, cat# 15596081). Total RNA was extracted and DNAse treated using the DirectZol kit(Zymo Research cat# R2060). RNA quality was confirmed by Bioanalyzer (Agilent) Individual RNA libraries were prepared with unique barcodes using the SMARTer Stranded Total RNA Sample Prep HI Kit (Clontech cat# 634874). Libraries were quantified by PCR using a PhiX library (Illumina cat# TG-110-3001) standard curve and then multiplexed at a 1:1 ratio.
identifiers.namespace: GEO
identifiers.id: GSM4413771