alias: GSM4416595
title: GSM4416595: PNT-Prdm1KO-D8; Mus musculus; RNA-Seq
library_construction_protocol: Cells at different induction time points of PNT were collected ad resuspended in DPBS with 0.04% BSA. Then, cells suspensions (500-1000 cells per microliter) were loaded on a Chromium Single Cell Controller (10x Genomics) to obtain single-cell gel beads in emulsion (GEMs) by using Single Cell 3' Library and Gel Bead Kit V2 (10x genomics, 120237). Captured cDNAs were lysed and the released RNA were barcoded through reverse transcription in singular GEMs (Zheng et al., 2017). Barcoded cDNAs were pooled and cleaned by DynaBeads® MyOne Silane Beads (Invitrogen, 37002D). Single-cell RNA-seq libraries were prepared by Single Cell 3' Library Gel Bead Kit V2 (10x Genomics, 120237) following the manufacturer's instruction. Sequencing was operated on an Illumina HiSeq X Ten
identifiers.namespace: GEO
identifiers.id: GSM4416595