alias: GSM3680768
title: GSM3680768: CHX_set2∆_rep2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument).
identifiers.namespace: GEO
identifiers.id: GSM3680768