alias: GSM2121092
title: GSM2121092: Control_Hist_chr13; Mus musculus; OTHER
library_construction_protocol: Cells were cross-linked with 1% formaldehyde for 10 min and quenched with glycine. Crosslinked chromatin was digested with 200 U BglII for 4 hours at 37°C for 4 hours and an additional of 200U BglII overnight at 37°C, while shaking at 900 rpm.  After that, 10 % SDS was added to the sample and incubated for 25 min at 65°C while shaking at 900 rpm to inactivate the enzyme. Chromatin ends were ligated 100 U T4 DNA ligase under diluted conditions that favor junctions between cross-linked DNA fragments. 50 U NlaIII was added to the mixture and incubated overnight at 37°C. After that, chromatin ends were re-ligated. 4C DNA samples were barcoded and sequenced on Illumina HiSeq 2500. The universal Illumina adaptors were added to the pimers of each chromatin sample for Solexa sequencing.
identifiers.namespace: GEO
identifiers.id: GSM2121092