alias: S4
title: S4_Fungal
design: Library preparation was performed at Genotypic Technology’s Genomics facility. 25 ng of Nanodrop quantified DNA for sample was used for amplifying ITS region of 18S with specific primers which also have a ‘tag’ sequence that are complementary to Illumina sequence adapter and index primers from the Nextera XT Index kit V2. This round of PCR generates single Amplicon of ~280-300 bp for ITS1 and 300-350bp for ITS2 respectively. In the next round of PCR (indexing PCR) Illumina sequencing adapters and dual Indexing barcodes are added using limited cycle PCR .The library was cleaned using HighPrep PCR (Magbio, Cat # AC-60050) magnetic beads and validated for quality by running an aliquot on D1000 Tapestation Tape (Agilent).
identifiers.namespace: SUB1952052
identifiers.id: S4