alias: Mina Stream anaerobic arsenic enrichment culture: Raw sediment
title: 16S Sequencing Raw Sediment
design: Metagenomic DNA from 10 g (wet weight) of sediment was extracted and purified using the PowerSoil DNA Extraction Kit (MO BIO Laboratories, USA) according to the manufacturers instructions. Quantification and quality of total DNA were determined by absorbance at 260 nm and 260/280 and 260/230 ratios, respectively, using a NanoDrop Spectrophotometer (NanoDrop Technologies). DNA was stored at -20°C until further processing. Illumina MiSeq platform was used to generate bacterial community taxonomic profiles. The 16S rRNA V3-V4 region was amplified by PCR using primers described by Klindworth et al. (2013), and amplicon libraries were prepared according to the manufacturers protocol
identifiers.namespace: SUB2328029
identifiers.id: Mina Stream anaerobic arsenic enrichment culture: Raw sediment