alias: GSM4271916
title: GSM4271916: WT_F_10714; Mus musculus; RNA-Seq
library_construction_protocol: Brown adipose tissue (BAT) was immediately harvested, snap-frozen in liquid nitrogen and then stored at −80°C. The tissues was homogenized with RNA extraction buffer (TRIZOL reagent; Life Technologies, CA) to yield total RNA following manufacturer instructions. RNA sequencing was performed on Illumina HiSeq2000 platform with 2×50bp paired-end sequencing configuration. Briefly, the quality of the total RNA was assessed using the Agilent 2100 Bioanalyzer followed by 2 rounds of poly A+ selection and conversion to cDNA. The TruSeq library generation kit was used to construct the cDNA library as per the manufacturer's instructions (Illumina, San Diego, CA). The cDNA library was quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems, Woburn, MA) prior to sequencing. Paired end 2 × 50 bp sequencing runs were performed in the Illumina HiSeq2500.
identifiers.namespace: GEO
identifiers.id: GSM4271916