alias: GSM3828518
title: GSM3828518: ARID1A WT_IFNγ_Rep1_ATAC_Seq; Homo sapiens; ATAC-seq
library_construction_protocol: Cell nuclei were isolated and subjected to transposase for 30 minutes under active shaking (Buenrostro et al., 2013; Corces et al., 2017). Then, the purified tagged genomic DNA was amplified to generate illumine NGS library with Nextera DNA Sample Preparation Kit (Illumina, #FC-121-1030). The DNA size selection was done with Ampure beads (Beckman Coulter). The library was measured by bioanalyzer. Sequencing was performed by paired-end 50-mer sequencing in replicates at University of Michigan with HiSeq 2500 (Illumina). Then, the purified tagged genomic DNA was amplified to generate illumine NGS library with Nextera DNA Sample Preparation Kit.
identifiers.namespace: GEO
identifiers.id: GSM3828518