alias: GSM2465927
title: GSM2465927: P27_HCT_G0; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and chromatine-DNA complexes were isolated with anti-p27 and anti-PCAF antibodies Libraries were prepared using the NEBNext ChIP-Seq Library Prep Reagent Set for Illumina kit (ref. E6200S) according to the manufacturer's protocol. Briefly, 10 ng of input and ChIP enriched DNA were subjected to end repair, addition of  "A" bases to 3' ends and ligation of PE adapters. All purification steps were performed using Qiagen PCR purification columns (refs. 5092816 and 50928006). Library size selection was done with 2% low-range agarose gel. Library amplification was performed by PCR on the size selected fragments. final libraries were analyzed using Agilent DNA 1000 chip to estimate the quantity and check size distribution, and were then quantified by qPCR using the KAPA Library Quantification Kit (ref. KK4835, KapaBiosystems) prior toamplification with Illumina's cBot. sequencing was done on the HiSeq2000, single Read, 50 nts
identifiers.namespace: GEO
identifiers.id: GSM2465927