alias: GSM2466417
title: GSM2466417: ATRA_H3K4me3_ChIP-seq_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were treated with 1% formaldehyde to crosslink. Then cell membrane were broken using lysis buffer (50mM HEPES-KOH, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1% SDS). Chromatin were resuspended using FA lysis buffer (50mM HEPES-KOH, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS) and fragmented by ultra-sonication processor (cole-parmer, USA). Immunoprecipitation were performed overnight using dynabeads (Thermo Fisher) pre-incubated with anti-H3K4me3 and H3K27ac antibodies (abcam, England). Library construction was performed using TruePrep DNA Library Prep Kit (Vazyme, China) according to the manufacture's protocol.
identifiers.namespace: GEO
identifiers.id: GSM2466417