alias: GSM2888578
title: GSM2888578: Activated T cell transfer colitis model_MEK inhibitor 1 mg/kg rep4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared using Isogen II (Nippongene) and further purified by the aid of RNeasy Mini Kit column (Qiagen) and DNaseI (Qiagen) to avoid genomic DNA contamination, according to the manufacturer's instructions. The polyA-RNA was isolated from 2 ug of total RNA using the Dynabeads mRNA Direct Purification Kit (Thermo Fisher Scientific). The mRNA fragmentation with RNaseIII and following steps were carried out according to Ion Total RNA-Seq Kit v2 recommendation (#4476286, Thermo Fisher Scientific).
identifiers.namespace: GEO
identifiers.id: GSM2888578