alias: GSM2046794
title: GSM2046794: TN04_P_KCl3; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq: Samples were collected and stored in 15 ml Trizol Reagent (Invitrogen). RNA extraction was performed followed by DNase treatment, mRNA selection, a second DNase treatment, reaction cleanup, concentration, and conversion to cDNA. RNA-seq: Barcode sequences were amplified from cDNA samples using primers to add Illumina sequencing adapters. DNA-seq: Cultures were spun down and libraries were isolated using Qiagen CompactPrep Maxi Kit. DNA-seq: Barcode sequences were amplified from plasmid DNA samples using primers to add Illumina sequencing adapters. ChIP-seq: We performed ChIP-Seq in RFX-DN transduced neurons using an anti-MYC A14 antibody (Santa Cruz, sc-789) after 0 or 1 hour of depolarization in 55mM KCl as described in (Kim et al., 2010), except performing sonication on a Covaris. ChIP-seq: As described in (Kim et al., 2010).
identifiers.namespace: GEO
identifiers.id: GSM2046794