alias: PlatyE-T5
title: 16S rRNA sequences: Platygyra daedalea
design: DNA was extracted from the samples following slight modifications of the protocol reported in Wilson et al. 2002. The hypervariable region V5-V6 of the 16S rRNA gene was amplified using the primers 784F [5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGGATTAGATACCCTGGTA-3] and reverse primer 1061R [5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCRRCACGAGCTGACGAC-3] containing Illumina adapters. The PCRs were conducted using the AmpliTaqGold 360 Mastermix and 0.4 M of each primer. The amplification cycles were set as following: 95C for 10 min; 30 cycles each at 95C for 30 sec, 57C for 1 min, 72C for 30 sec; a final extension at 72C for 7 min. The PCR products were then pooled and sent to Ramaciotti Centre for Genomics (UNSW, Sydney) for library preparation and sequencing on the Illumina MiSeq system. Amplicon sequences were processed using the QIIME2 pipeline.
identifiers.namespace: SUB5336533
identifiers.id: PlatyE-T5