alias: GSM2808311
title: GSM2808311: WT_expo_1; Bacillus subtilis; RNA-Seq
library_construction_protocol: RNA were extracted by standard Phenol/Chloroform extraction and precipitated in the presence of isopropanol. RNA pellets were resuspended in 75 µL H2O for 3 h at 4 °C, treated with QIAGen RNase-Free DNase and cleansed with Norgen Concentration Micro Kit as per manufacturer's instructions. RNA libraries and next generation sequencing were performed and benefits from the expertise of the High-throughput Sequencing Platform of I2BC (CNRS, Gif/Yvette, France). Sequencing was performed using single read 75bases with 30 million stranded reads per sample on a NextSeq 500 Illumina sequencer.
identifiers.namespace: GEO
identifiers.id: GSM2808311