alias: GSM2735685
title: GSM2735685: BL4C-anchor2-rep2; Homo sapiens; OTHER
library_construction_protocol: Cells were cross-linked with formaldehyde and then nucleuses were isolated for lysis. Then the chromatin was fragmented with restriction enzymes HaeIII and the proximal ends were ligated with biotin-labeled bridge linker in situ. A whole genome chromatin was extracted and sheared into 300-500 bp. Then the biotinylated junctions were captured by M-280 streptavidin magnetic beads. The DNA libraries were prepared according to the standard Illumina library construction protocol, including end repair, A-tailing and Y-adaptor ligation (annealing: 5P- GATCGGAAGAGCACACGTCTGAACTCCAGTCAC and TACACTCTTTCCCTACACGACGCTCTTCCGATCT). Then two rounds of PCR were followed: 4C viewpoint primer and adaptor sequence of I7 (GTGACTGGAGTTCAGACGTGT) for the first round, I5 with 4C viewpoint and I7 with barcodes for the second round. Then the libraries were sequenced on Illumina sequencer Hiseq X Ten.
identifiers.namespace: GEO
identifiers.id: GSM2735685