alias: GSM3532175
title: GSM3532175: C2ABR; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted with RNeasy Mini Kit (Qiagen). For library construction, polyA-containing mRNA molecules were isolated with magnetic beads. Following purification, the mRNA was fragmented into small pieces. The cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are then purified and enriched with PCR amplification. We then quantified the PCR yield by Qubit and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final library.
identifiers.namespace: GEO
identifiers.id: GSM3532175