alias: GSM3678936
title: GSM3678936: FP_PolII_ChIP-Seq; Mus musculus; ChIP-Seq
library_construction_protocol: Frozen kidneys were briefly homogenized. Then tissues were cross-linked with 1% formaldehyde in 1 mL PBS for 10 min at RT. The crosslink was quenched by adding 67 μL of 2.5 M glycine(150 mM final). Cells were washed with PBS and harvested using ChIP lysis buffer. Cells were then sonicated to obtain fragments (100-500 bp) with Bioruptor Sonicator. Immunoprecipitation was performed with Pol II antibody (CST, 14958). After elution and reversal cross-linking, DNA was purified for library preparation. Library construction were performed using VAHTS Universal DNA Library Prep Kit (Vazyme, ND607-02) and VATHS DNA Adapters set2 for Illumina (Vazyme Biotech, Cat.N802-01)
identifiers.namespace: GEO
identifiers.id: GSM3678936