alias: E-MTAB-3792:Sample 16
title: Illumina HiSeq 1000 sequencing; SmallRNAseq analysis of the human retina miRNome of 16 individuals
design: SmallRNAseq analysis of the human retina miRNome of 16 individuals
library_construction_protocol: In collaboration with the Fondazione Banca degli Occhi del Veneto (FBOV), we collected retina samples from 18 different donors. in compliance with the tenets of the Declaration of Helsinki and after obtaining the informed consent from the donorâs next of kin. The average post-mortem time of the 16 samples was 22 hours 40 min (ranging from 6 to 26 hours).  Briefly, an incision was performed on the sclera and the scleral tissue was cut and removed leaving the rest of the eye bulb intact. The RPE/choroid was then removed as an intact sheet, revealing the neural retina. The latter was gently pulled away from the underlying vitreous body that remained intact. The dissected retinal tissue was visually inspected to exclude any cross contamination with the pigmented RPE/choroid and was immediately submerged in RNA Stabilisation Reagent (RNA later; Qiagen) to stabilise RNA and preserve gene expression profiles. More details in Parekh, M. et al. A simplified technique for in situ excision of cornea and evisceration of retinal tissue from human ocular globe. Journal of visualized experiments : JoVE, e3765, doi:10.3791/3765 (2012). mirneasy QIAGEN TruSeq Small RNA library preparation (Illumina)
identifiers.namespace: TELETHON INSTITUTE OF GENETICS AND MEDICINE, TIGEM
identifiers.id: E-MTAB-3792:Sample 16
identifiers.namespace: Telethon Institute of Genetics and Medicine, TIGEM
identifiers.id: E-MTAB-3792:Sample 16