alias: E-MTAB-7215:Stalk 1_p
title: NextSeq 500 paired end sequencing; RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum
design: RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum
library_construction_protocol: Stalk cells were isolated from 24 h fruiting bodies by pipetting vigorously in pronase/EDTA, followed by deposition on 50 µm nylon mesh and extensive washing with KK2. The stalks retained on the mesh were then collected, placed on top of 5 ml 25% Percoll in KK2 and centrifuged at 10,000g for 5 minutes at 4ºC. Stalks were collected from the top layer, washed with 5 volumes of KK2 and pelleted at 4,000 x g for 3 minutes. RNeasy kit (QIAGEN) was used for extraction Libraries were prepared with Illumina Truseq stranded mRNA library preparation kit.
identifiers.namespace: School of Life Sciences, University of Dundee
identifiers.id: E-MTAB-7215:Stalk 1_p