alias: GSM3679894
title: GSM3679894: iTreg_Input_ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: Total CD4+ T cells were MACS-enrichment and FACS-sorted. Cells were then transferred to a 96-well plate coated with anti-CD3ε and antiCD28 antibodies and cultured in RPMI complete media supplemented with TGF-β for 4 days. 2 x 107 cells were chemically crosslinked and sonicated on a Branson 450-D sonicator to generate fragmented genomic DNA Chromatin immunoprecipitation was performed using the following antibodies: anti-BACH2 (N-2; Tohoku University), anti-JunD (sc-74; Santa Cruz) and (sc-586; Santa Cruz) For sequencing of immunoprecipitated DNA, DNA fragments were blunt-end ligated to Illumina adaptors, amplified, and sequenced by using the HiSeq 2000 platform (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM3679894