alias: E-MTAB-4508:ICE153_T2
title: Illumina HiSeq 2000 paired end sequencing; Natural variation of gibberellin responses in Arabidopsis thaliana
design: Natural variation of gibberellin responses in Arabidopsis thaliana
library_construction_protocol: For RNA sequencing analysis, seedlings (12 DAS for Col-0 and Ey15-2 and 13 DAS for WalhaesB4, ICE97, ICE138, ICE75, Ler-0, Yeg-1, Sha, ICE153) were harvested in RNA ice-later solution (AM7030; Ambion) and incubated at -20°C for at least a week.  Leaf 6 was micro-dissected on a cold plate with dry ice under a stereomicroscope, and frozen in liquid nitrogen. RNA was extracted with Trizol (Invitrogen) according to the manufacturer's instructions and the RNeasy kit (Qiagen) with on-column DNase (Qiagen) digestion. RNA was quantified and the quality was checked with a 2100 Bioanalyzer (Agilent).  Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, polyA containing mRNA molecules are reverse transcribed, double-stranded cDNA is generated and adapters are ligated.
identifiers.namespace: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
identifiers.id: E-MTAB-4508:ICE153_T2