alias: steffi_pgc_bs_experiment_0:0:1:0
title: E11.5 RNA-Seq experiment
design: RNA-Seq Expression Library Construction
library_construction_protocol: Between 30ng and 100ng total RNA was DNase treated with Ambion's DNA-free Kit according to the manufacturer's instruction. Enrichment for mRNA was performed using Dynabeads Oligo(dT)25 from Invitrogen in two subsequent steps of purification with fresh beads. The isolated mRNA was fragmented and converted into cDNA. For the library preparation, the NEB Next kit was used according to the manufacturer's instructions. Illumina PE adapters were ligated onto the end-repaired, and A-tailed cDNA. Libraries were amplified with 12 cycles and size selected by gel extraction for fragments between 200bp and 250bp.
identifiers.namespace: BI-UK
identifiers.id: steffi_pgc_bs_experiment_0:0:1:0