alias: GSM3680106
title: GSM3680106: K562_SC10; Homo sapiens; OTHER
library_construction_protocol: For bulk samples, DNA was extracted using Qiagen Dneasy Blood and Tissue kit. Single cells were stained with Calcein-AM dye and isolated into tubes. Cells were lysed and protease treated prior to HpaII digestion. Digested unmethylated sites were ligated with unmethylation-tag (U-tag) adapters. Excess adapters were then removed using NEB Thermolabile USER II enzyme. This was followed by the second round of digestion with MspI and NlaIII enzymes. Methylation-tag (M-tag) and Nla-tag (N-tag) adapters were ligated with the digested fragments. Excess adapters and oligonucleotides were removed with Thermolabile USER and Exonuclease enzymes. The Read1 and Read2 Illumina sequencing adapters were added through PCR. The amplified library was size selected using BluePippin and quantified using Kapa Library Quantification kit.  
identifiers.namespace: GEO
identifiers.id: GSM3680106