alias: GSM3473709
title: GSM3473709: 5_B2_R1_9wk; Homo sapiens; RNA-Seq
library_construction_protocol: At 4 weeks of age, one-half of the cultures were dissociated with Accutase, and subjected to negative selection using magnetic activated cell sorting (MACS) as described previously in Djuric Mol Cell Prot 2017 to deplete NPCs and glia. The flow-through (neuronal fraction) from MACS was harvested, and total RNA was extracted using a standard Trizol-based protocol. The quality of RNA was checked by BioAnalyzer Libraries prepared from the isolated RNA were sequenced on Illumina HiSeq 2500 with paired-end reads (125 bp each) to a depth of approximately 30 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM3473709