alias: GSM2319075
title: GSM2319075: A001; Mus musculus; RNA-Seq
library_construction_protocol: The total RNA from the colon tissues was extracted using TRIzol reagent (Invitrogen Life Technologies, Gaithersburg, MD) and purified with RNeasy mini kit (QIAGEN, GmBH, Germany) and RNase-Free DNase Set (QIAGEN, GmBH, Germany), and the experiment was performed according to the manufacturer’s protocol. The integrity and quality of the total RNA were checked by a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US), respectively. The ODA260/A280 of the RNA sample was between 1.9 and 2.1. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2319075