alias: GSM2051228
title: GSM2051228: Control #2 Placenta; Bos indicus x Bos taurus; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol Reagent (Invitrogen) according to the manufacturer's instructions. Semen DNA was isolated using phenol chloroform method. RNAseq libraries were prepared using Illumina TruSeq RNA sample preparation kit. Briefly, poly-A containing RNA was purified using magnetic beads with oligo-dT attached. The purified RNA was fragmented and used as a template for cDNA synthesis using random primers. In the following end-repair procedure, an "A" was added to the 3' end of the cDNA. The cDNA fragments with A-tailing were then ligated with T-tailing adaptors. PCR amplifications of cDNA fragments were followed to generate the final RNAseq library.  Two DNaseq libraries (one with 350bp target insert size, the other with 550bp target insert size) were prepared using Illumina TruSeq DNA PCR-Free Sample Preparation Kit according to the manufacturer’s instructions (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM2051228