alias: GSM3681466
title: GSM3681466: Nascent RNA-seq in THP1 cells 1; Homo sapiens; RNA-Seq
library_construction_protocol: 108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. see sample table DNA libraries were prepared using the NEB Next Ultra Directional RNA library preparation kit for Illumina (E7420).
identifiers.namespace: GEO
identifiers.id: GSM3681466