alias: GSM1870031
title: GSM1870031: +Pit Root 16 dat EXP; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: For RNA-Seq total RNA was isolated using the TRIZOL reagent (Invitrogen) according to the manufacturer's instructions and further purificated using the Qiagen RNeasy plant mini kit according to the manufacturer's instructions. For preparation of mRNA-Seq Libraries, total RNA was isolated from frozen shoot and root powder using the TRIzol® reagent (Invitrogen) according to the manufacturer’s instructions for isolation of total RNA from plant tissue and further purified using Plant RNeasy kit (Qiagen) according to the manufacturer’s instructions for RNA purification. Non–strand-specific mRNA-seq libraries were generated from 5 μg of total RNA (Plant RNeasy kit) and prepared by using the TruSeq RNA Sample Prep kit (Illumina) according to the manufacturer’s instructions.
identifiers.namespace: GEO
identifiers.id: GSM1870031