alias: GSM2046669
title: GSM2046669: hsa-miR-449b-5p human nasal epithelial cells; Homo sapiens; RNA-Seq
library_construction_protocol: Qiazol miRNeasy mini kit PolyA+ RNAs were selected from 2µg total RNA with Dynabeads mRNA purification kit (invitrogen). PolyA RNA were ligated, reverse transcribed and amplified (8 cycles) with the reagents from the NEBNext Small RNA Library Prep Set for SOLiD. Amplified libraries were subjected to 4 additional PCR rounds with the primers from the 5500 W Conversion Primers Kit (Life Technologies). Libraries were size-selected from 250 nt to 300 nt with the LabChip XT DNA 300 Assay Kit (Caliper Lifesciences). Libraries were converted with the enzyme kit from the 5500 W Conversion Primers Kit (Life Technologies) and quantified with the Bioanalyzer High Sensitivity DNA Kit (Agilent).
identifiers.namespace: GEO
identifiers.id: GSM2046669