alias: GSM2047237
title: GSM2047237: RNA-seq_10nM_replicate5; Xenopus laevis; RNA-Seq
library_construction_protocol: Each dish of embryos were harvested at stage 18 in five, 5-embryo aliquots, each of which was designated as one replicate. Each replicate embryos were homogenized in 200 uL/aliquot of TriPure. Total RNA was Dnase-treated, LiCl precipitated. Total RNA (100 ng) was reverse transcribed into cDNA using Nugen Ovation RNA Amplification System V2. The cDNA was converted to ABI SOLiD 5500xl deep sequencing libraries using SOLiD Fragment Library Core Kit with barcodes. RNA-seq, single read (50 nt), non-directional
identifiers.namespace: GEO
identifiers.id: GSM2047237