alias: GSM2311770
title: GSM2311770: SG-2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For SG &SC, the cDNA libraries were constructed following SMARTer Ultra Low Input RNA Kit for Sequencing-v3 (Clonetech, 634848) with 12 PCR cycles, and purified using the Agencourt Ampure XP Kit (Beckman Coulter, A63880). For tut,bam or bgcn mutants, total RNAs from tut (tut3), bam (bamBG/bamΔ86) or bgcn (bgcn20093/bgcnQS2) mutant testes were extracted using Trizol (Invitrogen, 15596-026).mRNAs were enriched from total RNAs using Dynabeads mRNA DIRECT Kit (Invitrogen, 61012). For SG&SC, The full-length cDNA outputs were processed with the Nextera XT DNA Sample Preparation Kit (Illumina, FC-131-1024). For tut, bam or bgcn mutant, RNA-seq libraries were generated using the mRNA-Seq Sample Preparation kit (Illumina, RS-930-1001) following manufacturer instructions.
identifiers.namespace: GEO
identifiers.id: GSM2311770