alias: GSM2808238
title: GSM2808238: MT_LEC; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq experiments with pooled RNA samples extracted from 2-3 biological replicates were performed. Total RNA was extracted from control and Dot1lΔEC skin LECs using RNeasy Plus Mini Kit (74134, Qiagen) and its amount and quality of the total RNA were evaluated using Bioanalyzer (Agilent). RNA samples with >7.0 RNA Integrity Number (RIN) value were used for RNA-Seq library preparation with the ScriptSeq v2 kit (Illumina) according to manufacturer's instruction. Libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2808238