alias: GSM3827175
title: GSM3827175: NMU-O-P (single cell RNA-Seq); Mus musculus; RNA-Seq
library_construction_protocol: NMU-o-P and NMU-o-D were enzymatically dissociated and cells were suspended in phosphate-buffered saline (PBS) with 0.04% bovine serum albumin (BSA, Ambion AM2616). 10,000 cells per condition were loaded onto a 10x Chromium Single Cell Controller chip B (10x Genomics) as described in the manufacturer's protocol (Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, PN-1000075). Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean up, cDNA amplification and library construction were performed following the manufacturer's recommendations
identifiers.namespace: GEO
identifiers.id: GSM3827175