alias: GSM3681533
title: GSM3681533: Sample 6 empty vector; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from sorted cells was isolated using RNeasy plus mini kit (Qiagen 74136) following the manufacturer's protocol. After isolation, RNAsecure (ThermoFisher AM7006) was added to the RNA and samples heated for 10 minutes at 60°C. Samples cooled down on ice and then placed in -80°C. The preparation of poly(A)+ cDNA sequencing libraries and RNA sequencing were carried out as described before in Styrkarsdottir, U. Nat Genet 2017 and Sigurdsson et al Nat Communications 2017.
identifiers.namespace: GEO
identifiers.id: GSM3681533