alias: GSM4416473
title: GSM4416473: Expt3_Input-KO clone 1; Homo sapiens; ChIP-Seq
library_construction_protocol: 3x10^7 cells were cross-linked with 1% formaldehyde at room temperature for 10 min, quenched with 0.125 M glycine and resuspended in SDS lysis buffer. The cells were sheared twice with a 27-gauge needle and sonicated in a Bioruptor (Diagenode, Denville, NJ) to generate chromatin fragments of 200–600 bp, followed by ChIP using H3K36me2 antibody catalog #ab9049 from Abcam, Cambridge, MA. The ChIP and input DNA samples were used for library construction (ThruPLEX library prep kit, Takara Bio, Mountain View, CA) and DNA sequencing on an Illumina HiSeq 4000 per the manufacturers' protocols using 50 nt single-end reads. The wild type and 4 KO samples from three biological replicate experiments were barcoded and run together in five lanes.
identifiers.namespace: GEO
identifiers.id: GSM4416473