alias: Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets
title: The V3-V4 region of the 16S rRNA gene was amplified from genomic DNA using the primers (338F 5′-barcode-ACTCCTACGGGAGGCAGCA-3′ and 806R 5′- GGACTACHVGGGTWTCTAAT-3′). The barcode is an eight-base sequence unique to each sample. PCR reactions were performed in triplicate in a 20-μL mixture containing 4 μL of 5× Fast Pfu buffer, 2 μL of 2.5 mM dNTPs, 0.4 mL of each primer (5 μM), 0.4 μL of Fast Pfu polymerase, and 10 ng of template DNA.
identifiers.namespace: Yangzhou University
identifiers.id: Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets