alias: GSM4272421
title: GSM4272421: A375_Top12_R1_HiC; Homo sapiens; Hi-C
library_construction_protocol: Briefly, 5 million of A375 cells subpopulations were fixed with 1% formaldehyde, suspended in cell lysis buffer for permeabilization, and homogenized by douncing. Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation, adaptor ligation, and PCR amplification steps were carried out on bead bound DNA libraries.
identifiers.namespace: GEO
identifiers.id: GSM4272421