alias: RM8378 MiSeq Vial 2 Rep 1
title: MiSeq Sequencing Data for NIST RM 8378 Clostridium sporogenes - Vial 2 Rep 1
design: Independent duplicate libraries were constructed from 8 replicate Clostridium sporogenes microbial genomic DNA reference material (RM8378) vials for a total of 16 libraries, the libraries were pooled and sequenced in a single run. Libraries were prepared using the Nextera DNA Sample Prep Kit (FC-121-1030). The 8 vials were barcoded using the Nextera Index Kit (FC-121-1011, lot: 9988423), vial numbers correlate with Ion Torrent data vial numbers. Libraries were sequenced using the the MiSeq 600 cycle Reagent kit v3 (MS-102-3003, box 1 lot:10064179; box 2 lot:10065188). Fastq generated using the MiSeq Reporter version 2.5.1.3 with the GenerateFASTQWorker workflow Version 0.0.0. Vial 2 Rep 1 barcoded: i5 index- N502, i7 index - N701.
identifiers.namespace: SUB1321331
identifiers.id: RM8378 MiSeq Vial 2 Rep 1