alias: GSM4416521
title: GSM4416521: GCMock; Rattus norvegicus; ChIP-Seq
library_construction_protocol: The enriched germ cells were subjected to sonication followed by immunoprecipitation using ERα and ERβ antibody. The immuno-precipitated DNA was quantified by Qubit2.0 and for size distribution Agilent Bioanalyser was used as a QC step for chip sequencing library. Chip Sequencing libraries were generated using NEBNext® Ultra™ II DNA kit for Illumina as per the recommended protocol. Briefly, a total amount of 200-500pg input material is used for Chip sequencing. The fragmented DNA overhangs were converted into blunt ends via exonuclease/polymerase activities (END REPAIR). After adenylation of 3' ends of DNA fragments, adapter oligonucleotides were ligated. DNA fragments with ligated adapter molecules on both ends were selectively enriched in final PCR reaction. Products were purified using AMPure XP system (Beckman Coulter, Beverly,USA) and quantified using the Agilent high sensitivity DNA assay on the Agilent Bioanalyzer 2100 system for size distribution. The final libraries are quantified using Kapa qPCR Kit for adapter ligated molecules and was sequenced on PE75 Illumina platform.
identifiers.namespace: GEO
identifiers.id: GSM4416521