alias: GSM3679919
title: GSM3679919: scRNA_seq_E14.5; Mus musculus; RNA-Seq
library_construction_protocol: Gonads were isolated and washed with PBS solution supplemented with 0.04% bovine serum albumin. Then, the gonads were digested with 1X TrypLE™ Express (Cat# 12605010) for 3 min to obtain single cell pellets. The single-cell Gel-Bead in Emulsions (GEMs) were then generated with Chromium 10x Genomics single-cell system by using single-cell 3' Library and Gel Bead Kit V2 (10x Genomics, Cat#120237). Captured cells were then lysed and the released RNA were barcoded through reverse transcription in individual GEMs. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics, 120237) following the manufacture's introduction.
identifiers.namespace: GEO
identifiers.id: GSM3679919