alias: GSM2466041
title: GSM2466041: W22-ref 2nd; Zea mays; miRNA-Seq
library_construction_protocol: Two pools of more than 10 cm tassels were from homozygous zmago18b mutants and wild-type sibling plants. Freshly dissected tassel tissue was dissected and immediately frozen in liquid nitrogen. Total RNA was extracted from pools of tassel tissue using Trizol (Life Technologies, Invitrogen, Carlsbad, CA, USA) and treated with RQ1 DNase (promega) to remove DNA. About 3 μg of total RNA was used for small RNA cDNA library preparation with Balancer NGS Library Preparation Kit for small/microRNA (GnomeGen) following manufacture's instruction. Briefly, RNAs were ligated to 3' and 5' adaptor sequentially, reverse transcribed to cDNA and PCR amplified. Whole library was applied to 10% native PAGE gel electrophoresis and bands corresponding to microRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified small RNA libraries were quantified with Qubit Fluorometer (Invitrogen, USA).
identifiers.namespace: GEO
identifiers.id: GSM2466041