alias: PEO4
title: PEO4 Exome Sequencing
design: For each cell line, 5 ug of genomic DNA was isolated for whole exome capture sequencing. In brief, DNA was fragmented (150-250 bp) and ligated to the paired-end adaptors. The adaptor-ligated fragments were then amplified by PCR and purified. Exon-containing fragments in these libraries were hybridized to the SureSelect Human All Exon Kit from Agilent technologies. This kit targets 165,637 exons (approx. 18,003 genes), totaling approximately 38 Mb of genomic DNA. The hybridized fragments were then captured using streptavidin coated magnetic beads, amplified and each sample was sequenced in the UT Southwestern Genomics Core Facility in two lanes of an Illumina GAIIx using a standard 75-bp paired-end protocol. The image analysis and base calling were performed using the Illumina pipeline with default settings.
identifiers.namespace: UT Southwestern
identifiers.id: PEO4