alias: Tcali.EL.mRNA.Dataset01.SUB1542866.SAMN05158845
title: Tcali.EL.mRNA.Dataset01.SUB1542866.SAMN05158845
design: Poly(A) mRNA was purified using Poly(A)Purist MAG Kit (Ambion) and further cleaned up with Turbo DNase (Ambion) and Terminal Exonuclease (Epicentre) to remove any trace amount of DNA and rRNA. cDNA was synthesized from purified poly(A) mRNA using random hexamer oligonucleotide or oligo dT as primer. Synthesized cDNA was then sheared into small pieces of 100~800 bp in length using a Biorupter. The fragmented cDNA was prepared for Illumina sequencing using TruSeq Sample Prep Kit (Illumina, San Diego, CA). Paired-end sequencing (100 bp) of the cDNA libraries was performed on a HiSeq 2500 instrument (Illumina.)
identifiers.namespace: Texas A&M University
identifiers.id: Tcali.EL.mRNA.Dataset01.SUB1542866.SAMN05158845