alias: GSM3595873
title: GSM3595873: Spermatogonia_Input_16; Mus musculus; ChIP-Seq
library_construction_protocol: Testes were removed and decapsulated. They were then digested with collagenase B (2mg/mL) for 10 minutes at 37°C. After rinsing in PBS, another digestion with 0.25% trypsin-EDTA for 5 minutes at 37°C was performed. The resulting cell suspension was washed twice, resuspended in sterile phosphate buffer solution (PBS) with DNase (50μg/mL) and filtered (40μm). Spermatogonia were isolated by fluorescence-activated cell sorting of Oct4-GFP positive germ cells with a FACSAria III laser sorter. ChIP-seq libraries were prepared using the Kapa HTP Library Preparation Kit (Roche) as per the manufacturer's recommendations except that adapters and PCR primers were diluted 100-fold and the size selection step was done after the PCR step.
identifiers.namespace: GEO
identifiers.id: GSM3595873