alias: E-MTAB-7020:Sample 7_p
title: Illumina HiSeq 2500 paired end sequencing; RNA-seq study on time of day specific Glucocorticoid action in mouse liver and lung tissues
design: RNA-seq study on time of day specific Glucocorticoid action in mouse liver and lung tissues
library_construction_protocol: Experimentation was performed on mouse strains C57BL/6J (WT) from Harlan Blackthorn, UK. Mice were treated at 6 hours ZT6 (6 hours after lights on, 1:30pm) or at ZT18 (6 hours after lights off, 1:30am) with dexamethasone (1 mg/kg intraperitoneal) or vehicle (methylcyclodextrin 1mg/kg intraperitoneal) Lung and, liver were lysed and total RNA prepared using SV Total RNA Isolation System (Promega). Quality and integrity of total RNA samples were assessed by 2100 Bioanalyzer or a 2200 TapeStation (Agilent Technologies) according to the manufacturer's instructions.  RNA sequencing (RNA-seq) libraries were generated using the TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol.
identifiers.namespace: Division of Informatics, Imaging and Data Sciences Faculty of Biology, Medicine, and Health University of Manchester, Manchester, UK
identifiers.id: E-MTAB-7020:Sample 7_p