alias: SH36rep2_S16_L002_R1
title: Aspergillus terreus transcriptome on soybean hulls 36h
design: Culture mycelia were harvested after a 36h growth period on media with soybean hulls as sole carbon source and immediately frozen in liquid nitrogen. Isolation of total RNA from frozen mycelia was performed according to Brasileiro & Carneiro (2015). RNA concentration and integrity were estimated with a NanoDrop 2000c spectrophotometer (Thermo Scientific, Waltham, MA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). Messenger RNA isolation, cDNA library preparation, and Illumina RNAseq were performed by Eurofins MWG Operon (Alabama, USA). cDNA libraries were prepared using the TruSeq Sample RNA preparation kit v3 Kit according to the manufacturer's specifications and paired-end-sequenced (2x 100 base pairs) in two flowcell lanes on an Illumina Hiseq2500 sequencer (Illumina Inc, San Diego, CA, USA).
identifiers.namespace: SUB7398873
identifiers.id: SH36rep2_S16_L002_R1