alias: GSM3828834
title: GSM3828834: 0127-V05; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Blood RNA Kit (PreAnalytiX, Switzerland), using the automated protocol (QIAcube instrument, QIAGEN, Germany). The ribodepleted and globin depleted fraction was selected from the total RNA using Ribo-ZeroTM Gold rRNA removal Kit (Illumina, USA). RNA was converted to cDNA, second-strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated and prior to amplification, samples underwent uridine digestion (to ensure strand-specific sequencing) The prepared libraries were size selected, multiplexed and quality controlled before 100bp paired-end sequencing (HiSeq4000). Sequencing was conducted at the Wellcome Trust Centre for Human Genetics (Oxford, UK).
identifiers.namespace: GEO
identifiers.id: GSM3828834