alias: GSM3679756
title: GSM3679756: Ad-PDX1+ Ad-DN-REST C; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in Tri-reagent and total RNA was extracted using the Direct-zol RNA miniprep kit (Zymo research, #R2052) Purified messanger RNA was eluted at 70C with 6ul of 10mM Tris-Cl pH 7.5 and stored at -80C.For library preparation RNA was used for the bulk MARS-seq  protocol (Jaitin et al. 2014 Science.343:776-9). MARS-seq libraries were sequenced using Illumina NextSeq 500 High Output v2 Kit (75 cycles).
identifiers.namespace: GEO
identifiers.id: GSM3679756