alias: 15
title: RNA-seq of mouse Lewis lung carcinoma P29mtP29 cybrid cells
design: Total RNA was extracted from P29mtP29 cells using RNAeasy Plus Mini Kit, with quality and quantity assessed onn an Agilent 2100 BioAnalyzer and RNA 6000 Nano Kit (criteria: 5 g of total RNA with RNA integrity  7); after depleting ribosomal RNA with RiboMinus Eukaryote System v2, the sample was barcoded with Ion Total RNA-Seq kit v2.0, quantified (High Sensitivity DNA Kit) and subject to emulsion PCR and sequenced on an Ion Torrent Proton sequencer.
identifiers.namespace: SUB7737648
identifiers.id: 15