alias: GSM2890514
title: GSM2890514: W4397 RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was extracted from snap frozen (TRAMP x WSB/EiJ) F2 bulk tumor samples using TRIzol Reagent (Life Technologies, Inc.) according to the manufacturer's protocol, and purified using RNeasy mini kit (QIAGEN) according to the manufacturer's protocol. RNA-Seq libraries were constructed from 1 μg total RNA after rRNA depletion using Ribo-Zero GOLD (Illumina). The Illumina TruSeq RNA Sample Prep V2 Kit was used according to manufacturer's instructions. The cDNAs were fragmented to ~275 bp using a Covaris E210. Libraries were  pooled together for sequencing. The pooled libraries were sequenced on multiple lanes of a HiSeq 2500 using version 4 chemistry to achieve a minimum of 43 million 126 base read pairs
identifiers.namespace: GEO
identifiers.id: GSM2890514