alias: GSM2466431
title: GSM2466431: control HL-60_RNA-seq_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq:Total RNA of ATRA treated and control cells were extracted using TRIZOL mRNA were isolated using poly(A) mRNA magnetic isolation module (NEB, England) and cDNA were got through reverse transcription and second strand synthesis (NEB, England). Then, cDNA were fragmented by S220 Focused-ultrasonicator (Covaris, USA). End repairing, adenine adding and adaptor ligation were performed using Enzymatics kit (Enzymatics, USA). PCR were performed using high-fidelity master mix (NEB, England).
identifiers.namespace: GEO
identifiers.id: GSM2466431