alias: GSM2210870
title: GSM2210870: H3K27me3 WT low Pi rep2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitation (ChIP) was performed using root (~1.5g) of A. thaliana (Col) following the published protocols (Anne-Valérie at al., 2005; http://www.nature.com/nmeth/journal/v2/n3/full/nmeth0305-213.html). For immunoprecipitation, 5 μg of antibodies against H3-core (Ab1791; Abcam), H3K4me3 (Ab8580; Abcam), H3K27me3(07-499; Upstate) and H3K9ac (Ab10812; Abcam), were used for ~25μg of chromatin extracts. DNA was purified using MinElute PCR purification kit (QIAGEN) for sequencing libraries preparation. DNA was quantified by Qubit and analyzed by bioanalyzer 2100. 2-5 ng ChIPed DNA was used to made ChIP-seq library. Libraries for ChIP-seq were prepared following KAPA Library Preparation Kits Illumina series (#KK8201, KAPA Biosystems) according to the manufacture’s protocol.
identifiers.namespace: GEO
identifiers.id: GSM2210870