alias: MQ171215-170_S170
title: 16S seq of culture day 21_20FLX
design: Ten ng of extracted DNA was used as template and the PCR reaction (25 uL) contained dNTPs (400nM of each), MgSO4 (1.5 mM), Platinum Taq DNA polymerase HF (2mU), 1X Platinum High Fidelity buffer (Thermo Fisher Scientific, USA), and barcoded library adaptors (400 nM) containing V1-3 specific primers: 27F AGAGTTTGATCCTGGCTCAG and 534R ATTACCGCGGCTGCTGG.
identifiers.namespace: SUB4428215
identifiers.id: MQ171215-170_S170