alias: GSM3304014
title: GSM3304014: P4_Normal; Homo sapiens; RNA-Seq
library_construction_protocol: Cellular suspensions were loaded on a Chromium Single Cell Controller (10x Genomics, Pleasanton, CA, USA) to generate single-cell GEMs. Single-cell RNA-Seq libraries were prepared using Chromium™ Single Cell 3' Library & Gel Bead Kit (10x Genomics, P/N-120237).  GEM-RT was performed in a Biometra Thermal cycler with TempAssure PCR 8-tube strip (USA Scientific, P/N 1402-4700): 53 °C for 45 min, 85 °C for 5 min; held at 4 °C. Constructed using the reagents in the Chromium™ Single Cell 3' Library & Gel Bead Kit, following these steps: (1) end repair and A-tailing; (2) adapter ligation; (3) post-ligation cleanup with SPRIselect; (4) sample index PCR and cleanup.  The barcode sequencing libraries were quantified using the Qubit 3.0 (Thermo Fisher, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies).
identifiers.namespace: GEO
identifiers.id: GSM3304014