alias: GSM1673657
title: GSM1673657: MEL_DNase_D5R2; Mus musculus; OTHER
library_construction_protocol: Biological replicates of DNase-seq libraries for uninduced and induced MELs were built as previously described except for the DNA purification below (Hesselberth et al. 2009). Briefly, 20 million nuclei were digested with 80U/ml DNaseI (Catalog number D4527, Sigma-Aldrich, St.Louis, MO) for 3 minutes at 37 degree Celsius. Following 1-hour digestion with proteinase K (Sigma-Aldrich), DNA samples were purified by phenol/chloroform (Fisher Scientific, Waltham, MA) and precipitated by isopropanol (Fisher Scientific) and sodium acetate, pH 5.5 (Life Technologies). DNA fragments between 25 and 100bp were purified by gel electrophoresis and constructed into sequencing libraries. MEL total RNA was extract by RNeasy Kit (QIAGEN) and the quality was checked by RIN.
identifiers.namespace: GEO
identifiers.id: GSM1673657