alias: E-MTAB-3435:+Nar2
title: AB SOLiD 3 Plus System sequencing; Genome wide transcriptional profiling of Herbaspirillum seropedicae SmR1 grown in the presence of naringenin
design: Genome wide transcriptional profiling of Herbaspirillum seropedicae SmR1 grown in the presence of naringenin
library_construction_protocol: H. seropedicae SmR1, streptomycin resistant, was  grown at 30 degrees C and 120 rpm in NFbHPN medium (Klassen et al., 1997) in the presence (+Nar) or absence (-Nar) of 100 uM of naringenin containing streptomycin (80 ug.mL-1)for 6 hours (optical density at 600 nm of 0.8). Cells were collected by centrifugation. The total RNA was isolated using RiboPureTM-Bacteria Kit (Ambion) After rRNA depletion 500 ng of depleted rRNA was used to construct the sequencing libraries following standard protocols using the SOLiD Total RNA-Seq Kit (Life Technologies). The libraries were barcoded by using the SOLiD Transcriptome Multiplexing Kit (Life Technologies).
identifiers.namespace: UFPR
identifiers.id: E-MTAB-3435:+Nar2