alias: GSM2122802
title: GSM2122802: GRHL2_ChIP-seq_replicate1; Homo sapiens; ChIP-Seq
library_construction_protocol: LNCaP cells were seeded at 3x10^6 cells/plate in 15cm plates in RMPI 1640 + 10% FBS and allowed to grow for 3 days prior to fixation with formaldehyde. Nuclear pellets were sonicated on ice for 10 cycles (30 sec on, 30 sec off) using a Diagenode Bioruptor. Lysates were immunoprecipitated with 4 ug GRHL2 antibody (Sigma HPA004820) or 5 ug AR antibody (Santa Cruz N20 ), which had been pre-coupled to Protein G Dynabeads (Life Technologies, 10004D). DNA was purified using a PCR purification kit (Qiagen) and eluted in 100 µl. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Prep Kit
identifiers.namespace: GEO
identifiers.id: GSM2122802