alias: GSM4663515
title: GSM4663515: HCT116_shDHX30_NUTLIN_TOT_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Polysomal separation was performed as follows: samples were loaded in 15-50% linear sucrose gradients, ultra-centrifuged and fractionated with an automated fraction collector. All the fractions containing subpolysomal or polysomal RNA were identified and pooled in two separate tubes. RNA was purified by extraction with 1 volume of phenol-chloroform and, after isopropanol precipitation, a washing step in 70% v/v ethanol was performed in order to remove phenol contaminations. RNA libraries were prepared for sequencing using standard Illumina protocols: Illumina TruSeq RNA Sample Prep Kit was used with 1.5 ug of total RNA as input.
identifiers.namespace: GEO
identifiers.id: GSM4663515