alias: AC29
title: Asbestos-induced mesothelioma tumour cell line AC29 p31
design: DNA was extracted using standard isopropanol purification. Genomic DNA was fragmented using an S2 sonicator (Covaris, USA). Whole genome libraries were constructed using a NEBNext Ultra DNA Library kit (New England Biosciences, Ipswich, MA) with local modifications (specifically Ion Torrent sequencing adaptors were added) and the exome was enriched using a SureSelectXT Mouse All Exon kit (Agilent, Mulgrave, Vic, Australia) that targets 49.6 Mb of the murine genome (221,784 exons across 24,306 coding genes). Sequencing was performed using a Proton sequencer (Ion Torrent, Thermofisher Scientific) system according to manufacturer’s instructions. Two samples were pooled and sequenced on a P1 chip for 520 flows to an average depth of 42x.
identifiers.namespace: University of Western Australia
identifiers.id: AC29