alias: GSM2891362
title: GSM2891362: single cell RNA-seq batch 6; Mus musculus; RNA-Seq
library_construction_protocol: Single HSCs were index sorted into cold 384-well capture plates containing 2 ul of cell lysis solution and barcoded poly(T) RT primers for scRNA-seq. cDNA from barcoded single cells was pooled using an automated pipeline  (Jaitin, D.A., et al. Science 2014). The pooled sample was amplified in-vitro and the resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with pool barcodes and Illumina sequences during subsequent steps (ligation, RT, and PCR) (Jaitin, D.A., et al. Science 2014)
identifiers.namespace: GEO
identifiers.id: GSM2891362