alias: GSM2211049
title: GSM2211049: p19R3; Nicotiana benthamiana; RNA-Seq
library_construction_protocol: Total RNA from N. benthamiana leaves was extracted using the RNeasy plant mini kit (QIAGEN, Leusden, The Netherlands) coupled with on-column DNase I treatment, following the manufacturer’s guidelines.  Total RNA integrity (RIN>8) was assessed using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA purity was assessed using a Nanodrop ND1000 spectrophotometer (Thermo scientific, Villebon-sur-Yvette, France). Total RNA was quantified using a Qubit RNA assay kit (Life technologies, Carlsbad, CA, USA). cDNA libraries were prepared from 10ng mRNA using the SMARTer Stranded RNA-Seq kit according to the manufacturer’s guidelines (ClonTech laboratories, Mountain View, CA, USA). Libraries were analyzed and quantified as described in Legay et al. 2015. The pooled libraries were sequenced on an Illumina MiSeq using 5 consecutive runs (Illumina MiSeq reagent V3-150 cycles) to generate 76 base-pairs paired-end reads.
identifiers.namespace: GEO
identifiers.id: GSM2211049