alias: GSM2465340
title: GSM2465340: mCN_LSD1_shRNA_B_DLAR_BRUSEQ_rep2; Mus musculus; OTHER
library_construction_protocol: Nuclei in Trizol were homogenized with a qiashredder. Direct ligation of 3' adaptors to RNA and cDNA (DLAR). 3' adaptor ligation to the fragmented RNA followed by anti-BrdU enrichment and reverse transcription. Second adaptor ligation to 3' of cDNA. Purified RNA was fragmented and reverse adaptor was ligated to the 3' end of RNA. BrU containing RNA were enriched and reverse transcribed. After treatment with RNAses to remove hybrid RNA, splinted forward adaptor was ligated to the 3' ends of cDNA. Libraries were amplified by PCR.
identifiers.namespace: GEO
identifiers.id: GSM2465340