alias: GSM2888488
title: GSM2888488: LoVo MEK inhibitor 1 nM rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Extraction of total RNA was performed according to the manufacturer's instructions (RNeasy, QIAGEN) 10 ng of total RNA from each dose and biological replicate were reverse transcribed to cDNA with SuperScript® VILO™ cDNA Synthesis Kit (Thermo Fisher Scientific). Then cDNA is amplified using Ion AmpliSeq™ technology (Thermo Fisher Scientific). After primer digestion, adapters and molecular barcodes were ligated to the amplicons followed by magnetic bead purification. Libraries were then diluted to 100 pM and pooled equally, with eighteen individual samples per pool.
identifiers.namespace: GEO
identifiers.id: GSM2888488