alias: GSM3305227
title: GSM3305227: colon: tumor1; Mus musculus; RNA-Seq
library_construction_protocol: Mouse tumor fragments were isolated using a filtering protocol (see paper). Mouse epithelial cell suspensions were obtained by EDTA extraction and Collagenase/DNAse dissociation. Viable cells were enriched with MACS. Cells were encapsulated using the inDrop platform (1CellBio), and RNA was extracted according to the protocol of Klein et al., 2015. CEL-Seq linear amplification of RNA/cDNA followed by index primer-based amplification RNA-Seq was performed on NextSeq500 with a 2 x 75 paired-end kit using custom read lengths
identifiers.namespace: GEO
identifiers.id: GSM3305227