alias: GSM3377790
title: GSM3377790: Fasted_72 hrs; Mus musculus; RNA-Seq
library_construction_protocol: Ileum were removed, flash frozen in liquid nitrogen, and RNA was harvested using Total RNA Miniprep Purification Kit (GeneMarkbio,Cat# TR01). cDNA was generated using random N6 primer followed by A-tailing. The adaptors were ligated to the A-tailed cDNA, then were amplified by PCR. The PCR products were heated to separate into single strand DNA which was cyclized with splint oligonucleotides to generate libraries.
identifiers.namespace: GEO
identifiers.id: GSM3377790