alias: C.4
title: 16S sequencing of mouse cecal microbiota  of mice fed low or high fat,high sucrose diet supplemented with or without tart cherry
design: Total DNA was extracted using Mo-Bio (now Qiagen) PowerFecal kit. Sample libraries were prepared and analyzed by barcoded amplicon sequencing. In brief, the purified DNA was amplified on the V4 region of the 16S rRNA genes via PCR using the following primers: F319 (5-ACTCCTACGGGAGGCAGCAGT-3) and R806 (5-GGACTACNVGGGTWTCTAAT-3).  High-throughput sequencing was performed with Illumina MiSeq paired end 250-bp run.
identifiers.namespace: SUB5333048
identifiers.id: C.4