alias: GSM4546302
title: GSM4546302: Pat02-A; Homo sapiens; RNA-Seq
library_construction_protocol: Human stomach tissues were cut into approximately 1 mm3 pieces and add the 5 ml of dispase in Hanks' Balanced Salt Solution (STEMCELL Cat# 07913) and 5 mg collagenase type IV (Sigma Cat# C5138) and incubate at 37 °C for 2 h with gently shaking. After the sample was passed through a 100 m cell strainer (Falcon), the suspended cells in PBS (with1 mM EDTA) were centrifuged at 720 g for 5 min. To remove RBC in the sample, cell pellets were re-suspended PBS (with1 mM EDTA) and centrifuged through a discontinuous 50% Percoll® (Sigma Cat# P4937) gradient, at 470 g for 20 min, with minimum acceleration and no deceleration. The sample between layers PBS and 50% Percoll® was collected re-suspended in PBS for scRNA-seq. 10x signle cell 3' v2 kit scRNA-seq
identifiers.namespace: GEO
identifiers.id: GSM4546302