alias: GSM3530662
title: GSM3530662: Breast-N3-mRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol and Direct-zol RNA Kits (Zymo Research). After two rounds of DNase I treatment, 1 ug of total RNA was used to synthesize the cDNA. cDNA was purified with Axygen AxyPrep Mag PCR clean-up beads (Axygen). Each cDNA library (300 - 500 ng) was preamplified using a primer pool (50 uM each) covering all human miRNAs. Preamplified product was purified using Axygen AxyPrep Mag PCR clean-up beads. Preamplified cDNAs and primer pools were loaded into the 48.48 Access Array IFC (Fluidigm) and amplified as described before22. PCR products of each sample were subject to 15 cycle barcoding PCR and pooled together. All pools were combined at equal volumes and purified via Axygen AxyPrep Mag PCR clean-up beads.
identifiers.namespace: GEO
identifiers.id: GSM3530662