alias: GSM3827096
title: GSM3827096: murine CD8+ T cells 1 d activated by aCD3/CD28 rep1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in RLT-buffer supplemented with beta-Mercaptoethanol and total RNA was isolated according to the manual (Rneasy plus mini kit; Qiagen) Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Nano chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 20 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA assay.
identifiers.namespace: GEO
identifiers.id: GSM3827096