alias: GSM1677454
title: GSM1677454: Wildtype brain 3; Mus musculus; RNA-Seq
library_construction_protocol: Whole brains were collected from CO2-asphyxiated wildtype and Abcc5-/- mice, and immediately frozen on dry ice. After homogenization in Trizol (Invitrogen), RNA was extracted according to the manufacturer’s protocol. Sequencing libraries were generated according to the manufacturer's instructions (TruSeq RNA Sample Preparation v2 Guide; Illumina; Part # 15026495 Rev. F). Briefly, polyadenylated RNA from intact total RNA was purified using oligo-dT beads. Following purification the RNA was fragmented, random primed and reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen, part # 18064-014). Second strand synthesis was performed using Polymerase I and RNaseH. The resulting cDNA was converted to blunt end fragments using an end repair mix (Illumina), followed by 3' end adenylation and ligated to Illumina paired-end sequencing adapters and subsequently amplified by 16 cycles of PCR.
identifiers.namespace: GEO
identifiers.id: GSM1677454