alias: GSM3377786
title: GSM3377786: Fasted_48 hrs RPF; Mus musculus; RNA-Seq
library_construction_protocol: Ileal tissues taken from fed and fasted mice were lysed with lysis buffer and treated with nuclease. Ribosome-protected fragments (RPF) were separated with a sucrose-cushion. Footprint fragments were purified and used to generate deep sequencing libraries. After rRNA depletion DNA was PCR amplified with Illumina primers for 10 cycles and library fragments of ~175 bp were band isolated from a polyacrylamide TBE-urea gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
identifiers.namespace: GEO
identifiers.id: GSM3377786