alias: GSM2049344
title: GSM2049344: U2OS_exp2_OmoMYCmutHER_+Dox_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-sequencing, total RNA was extracted using the RNeasy mini columns (Qiagen) including on-column DNase I digestion. PolyA+-RNA was isolated from total with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB). The NEBNext® Ultra™ RNA Library Prep Kit for Illumina was used for library preparation following the manufacturer’s instruction. Size selection of the libraries was performed with the AMPure XP Beads (Beckman Coulter), followed by 12 PCR cycles for amplification. Library quantification and quality was assessed using the Experion Automated Electrophoresis System (Bio-Rad).
identifiers.namespace: GEO
identifiers.id: GSM2049344