alias: E-MTAB-7604:GC058849.count_s
title: Illumina HiSeq 4000 sequencing; RNA-sequencing of inflamed intestinal mucosa of inflammatory bowel disease patients
design: RNA-sequencing of inflamed intestinal mucosa of inflammatory bowel disease patients
library_construction_protocol: Biopsies at the edge of an ulcer were taken during endoscopy prior to the start of therapy, stored in RNALater buffer (Ambion, Austin, TX, USA) and preserved at -80°C.  Total RNA from inflamed biopsies was extracted using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The integrity and quantity of all RNA was assessed with a 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). Extracted RNA was stored at -80°C until further processing. Library preparation using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer's instructions.
identifiers.namespace: KU Leuven, Department of Chronic Diseases, Metabolism and Ageing
identifiers.id: E-MTAB-7604:GC058849.count_s