alias: GSM3473349
title: GSM3473349: input DNA; Mus musculus; ChIP-Seq
library_construction_protocol: The CD45+CD11c+SiglecF+  lung alverolar macrophages (AMs) were sorted by FACS Aria II flow cytometer. Cell pellets from the freshly isolated AMs were directly frozen at -80°C. The samples were then submitted to EpiGentek for ChIP, library preparation, and sequencing. The cells were fixed with 1% formaldehyde and chromatin was isolated using ChromaFlashTM Chromatin Extraction Kit. Chromatin was sheared using the Episonic2000 Sonicatin System. ChIP was performed using an antibody against HDAC3, H3K27ac or H3K9ac on the chromatin and Input DNA (without immunoprecipitation) was used as background. After adapter linking, library was size-selected (100-300 bp) and PCR-amplified. Ten nanomolar of sample libraries were provided for next generation sequencing.
identifiers.namespace: GEO
identifiers.id: GSM3473349