alias: GSM2467192
title: GSM2467192: 0 h; Nostoc punctiforme PCC 73102; RNA-Seq
library_construction_protocol: RNA extractions were performed as previously described with a bead beater and phenol (Campbell et al. 2007. Global gene expression patterns of Nostoc punctiforme in steady-state dinitrogen-grown heterocyst containing cultures and at single time points during the differentiation of akinetes and hormogonia. J. Bacteriol 189:5247-5256) Total RNA was ribodepleted using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, Inc.). cDNA was synthesized from the rRNA depleted samples, sheared to a library size of 200 bp, and appended with adapters following standard protocols at the University of California Berkeley QB3 Vincent J. Coates Genomics Sequencing Laboratory .
identifiers.namespace: GEO
identifiers.id: GSM2467192