alias: GSM2311199
title: GSM2311199: 15072801--bnt-D-NC14-12; Drosophila melanogaster; OTHER
library_construction_protocol: Embryos were observed under a fluorescent microscope until they reached the desired timepoint. Embryos were dechorionated in 4% bleach for 1 minute, washed extensively with water, and macerated in a small volume of extraction buffer. Nuclear pellets were collected and briefly frozen on dry ice. Frozen nuclear pellets were thawed and fragmented using the Nextera Tn5 transposase solution (Illumina), 2.5 ul per 10 ul reaction. Fragmentation was for 30 minutes at 37 degrees with shaking. Samples were cleaned up with a Qiagen minelute kit and subjected to limited PCR amplification with barcoded primers.
identifiers.namespace: GEO
identifiers.id: GSM2311199