alias: GSM2122553
title: GSM2122553: HTR siCon rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted from cells using PureLink RNA Mini Kit. RNA transcripts are enriched by selectively depleting ribosomal RNA molecules using the Ribo-Zero Gold Kit (Epicentre) and then sheared by incubation at 94C°. Following first-strand synthesis with random primers, second strand synthesis is performed with dUTP for generating strand-specific sequencing libraries. The cDNA library is then endrepaired, and A-tailed, adapters are ligated and second-strand digestion is performed by Uricil-DNA-Glycosylase. Indexed libraries that meet appropriate cut-offs for both are quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the Perkin Elmer LabChip GX or Agilent Bioanalyzer. Samples with a yield of ≥0.5 ng/ul are used for sequencing.
identifiers.namespace: GEO
identifiers.id: GSM2122553