alias: GSM3305272
title: GSM3305272: [ESI+]_1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Hot acid phenol extraction of total RNA Dynal Oligo-dT bead enrichment followed by reverse transcription and subsequent cDNA fragmentation using a Covaris E220. Fragment distribution was 200-400 bp, with a peak around 300 bp. Fragmented cDNA was then processed into a sequencing library using the RNA HyperPrep Kit (cat. KK854) and Kapa Biosystems Library Amplification kit (KK2612). Adaptors and barcodes used synthesized as single-stranded oligonucleotides and were Illumina TruSeq-compatible. Library quantification was performed using Kapa Library amplification kit (cat.KK4824), with final QC being performed by Agilent Bioanalyzer. Paired-end sequencing was performed on an Illumina NextSeq 500
identifiers.namespace: GEO
identifiers.id: GSM3305272