alias: GSM2735140
title: GSM2735140: RNA Bind-N-Seq: YTH purification ; sample 1; synthetic construct; OTHER
library_construction_protocol: For RNA Bind-n-Seq experiment  randomized 21 nt single-stranded RNAs carrying a central m6A moiety were mixed with Ni-NTA agarose beads alone or bound to 6xHis-SUMO-YTH domain (of hYTHDC2). RNA bound to the beads was phenol chloroform extracted. Sequencing libraries  of RNA Bind-n-Seq and m6a IP experiments were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions and sequenced on Illumina 2000 HiSeq platform (EMBL Heidelberg Gene core facility).
identifiers.namespace: GEO
identifiers.id: GSM2735140