alias: GSM1666854
title: GSM1666854: ATF4-/- ER mrna, untreated (rep 1); Mus musculus; RNA-Seq
library_construction_protocol: Cells fractionated between cytosol and ER using digitonin and DDM. Ribosome profiling samples: digested with micrococcal nuclease, pellet ribosomes, end polish with PNK, gel purify ribosome footprint. mRNA samples: Deplete rRNA using RiboZero, fragment RNA 20 min 95C in Mg-containing buffer, end polish with PNK. NEB Directional Small RNA kit. Libraries purified by gel electrophoresis.
identifiers.namespace: GEO
identifiers.id: GSM1666854