alias: GSM1673352
title: GSM1673352: SD_2; Mus musculus; RNA-Seq
library_construction_protocol: Eyes were harvested and rapidly dissected in the dark. The anterior portion of the eye including the lens was removed, and the remaining tissue (posterior sclera, choroid, RPE, and retina) was rinsed in cold sterile HBSS with calcium and magnesium (Gibco) and stored in TRIzol (Invitrogen) at -80 C. For extraction, the tissue was homogenized with a pestle and then passage through a needle. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). Polyadenylated mRNA was obtained from total RNA using Dynabeads (Invitrogen), fragmented, and reverse-transcribed to cDNA using random hexamers. The cDNA was blunt-ended, 3’-adenylated, and ligated to sequencing adapters. Ligated fragments were amplified for 12 cycles with primers to incorporate sample indices. All four samples were run on a single lane of HiSeq 2500 (1x50 bp).
identifiers.namespace: GEO
identifiers.id: GSM1673352