alias: E-MTAB-55011536939352:LMPP.Normal.ST.CB1_p
title: Illumina HiSeq 2000 paired end sequencing; RNA-Seq of human AML samples, and human cells transformed with MLL-ENL or MLL-AF6
design: RNA-Seq of human AML samples, and human cells transformed with MLL-ENL or MLL-AF6
library_construction_protocol: Cells were either uncultured, short-term (ST) or long-term (LT) cultured in IMDM+15%FBS+20ng/ul of SCF, TPO, FLT3L, IL-3 and IL-6 50-100 ng (RNAeasy micro, Qiagen) or 300-1000ng (mirVANA, Ambion) of isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina) and sequenced on HIseq2000 platform (Illumina) as per manufacturers recommendations.
identifiers.namespace: Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King's College London
identifiers.id: E-MTAB-55011536939352:LMPP.Normal.ST.CB1_p