alias: GSM3678505
title: GSM3678505: D6_p9_KO_3; Mus musculus; RNA-Seq
library_construction_protocol: Mouse lungs were homogenized in liquid nitrogen. 1 ml Trizol was added per 50-100 mg of tissue and the homogenate was incubated at room temperature for 5 minutes. 200 μl chloroform were added per ml of Trizol, shaken vigorously for 15-30 seconds and incubated at room temperature for 15 minutes. Samples were centrifuged for 15 minutes at 4˚C and 12,000 rcf. The aqueous phase was carefully collected in a separate tube. 500 μl isopropanol was added per 1 ml Trizol, inverted to mix and incubated at room temperature for 5-10 minutes. Samples were transferred to Qiagen RNeasy Spin Columns and RNA was isolated according to the manufacturer's instructions. Libraries were constructed using the Illumina TruSeq Stranded Total RNA Library Prep Kit.
identifiers.namespace: GEO
identifiers.id: GSM3678505