alias: GSM1671786
title: GSM1671786: Dicerfl/fl infected MEFs rep1; Mus musculus; RIP-Seq
library_construction_protocol: Cells were UV Crosslinked, and then lysed. The lysates then underwent Rnase A treatment and AGO IP on bead. AGO-bound RNA was 32-P labeled with a 3' linker ligated. Resulting products were run on a SDS-PAGE gel, the nitrocellulose membrane was exposed, and AGO+RNA products (110 kDa, 130kDa) were excised, with protein and DNA digested, and RNA extracted To the 3' linker ligated RNA extracted with the protocol described above, 5' linker was added. The resulting products were PCR amplified, adding sequencing adapters, and then multiplexed for sequencing with Illumina HiSeq 2000 single end
identifiers.namespace: GEO
identifiers.id: GSM1671786