alias: GSM3532128
title: GSM3532128: Hi-C WT rep1; Homo sapiens; OTHER
library_construction_protocol: Cells were lysed and DNA was then cut with MboI and the overhangs were filled with a biotinylated base. Free ends were then ligated together in situ. Crosslinks were reversed, the DNA was sheared to 300-500bp and then biotinylated ligation junctions were recovered with streptavidin beads. Sequencing libraries were generated using standard Illumina library construction protocol. Briefly, ends of sheared DNA were repaired and the blunt ends were added an “A” base to ligate with Illumina's adapters that have a single 'T' base overhang. Then DNA was PCR amplified for 8-12 cycles. At last, products were purified using AMPure XP system and sequenced through XTen (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM3532128