alias: GSM2891349
title: GSM2891349: Bulk RNA-seq HSC CFP Dim samples 1; Mus musculus; RNA-Seq
library_construction_protocol: HSCs were separated into three groups depending on their CFP levels (~20% brightest, dimmest and an intermediate group) and 400-1000 cells were sorted into 50 ul of LiDS lysis/binding buffer (Life Technologies). mRNA was captured with 12 ul of Dynabeads oligo(dT) (Life Technologies), washed and eluted with 10 ul of 10 mM Tris-HCl buffer (pH 7.5) To produce gene expression libraries, a derivation of the protocol used for generating the single cell expression libraries was used (Jaitin, D.A., et al. Science 2014).
identifiers.namespace: GEO
identifiers.id: GSM2891349