alias: GSM2809900
title: GSM2809900: DMSO2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cell cultures with TRIzol reagent (Invitrogen), purified with an RNeasy kit (Qiagen) according to manufacturer's instructions mRNA is enriched using oligo(dT) beads, and then fragmented randomly in fragmentation buffer, followed by cDNA synthesis using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) is added with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation. The final cDNA library is ready after a round of purification, terminal repair, A-tailing, ligation of sequencing adapters, size selection and PCR enrichment.
identifiers.namespace: GEO
identifiers.id: GSM2809900