alias: E-MTAB-6281:Procyclic ZC3H30 DKO10B_s
title: Illumina HiSeq 2000 sequencing; RNA-Seq data: RNA-IP (TAP-ZC3H30 pull down followed by RNA-Seq) and total transcriptomics (Effect of ZC3H30 gene deletion) in procyclic form of T. brucei
design: RNA-Seq data: RNA-IP (TAP-ZC3H30 pull down followed by RNA-Seq) and total transcriptomics (Effect of ZC3H30 gene deletion) in procyclic form of T. brucei
library_construction_protocol: Total RNA protocol from non-heat shocked cells. All procyclic cells were grown at log phase till 1.5 million cells/ml but not more than 2 million cells/ml. A total of 5million cells were harvested at 850xg, 8 minutes, washed with cold 1xPBS, and then proceeded to RNA extraction using TriFast  from PeqLab. All procyclic cells were grown at log phase till 1.5 million cells/ml but not more than 2 million cells/ml. Grown at normal growth temperature of 27 degrees. Log phase cell culture was heat shocked at 39 degrees for 1 hour before RNA extraction. Stand chloroform RNA extraction using TriFast from PeqLab. Standrd Illumina library preparation protocol.
identifiers.namespace: ZMBH
identifiers.id: E-MTAB-6281:Procyclic ZC3H30 DKO10B_s