alias: R2D
title: RNA-seq of tetrahymena thermophila: mating pool
design: Total RNA in the 2 doubling stage after refeeding were purified using TriPure Isolation Reagent (Roche, Indianapolis, IN) and the purified RNAs were further concentrated using RNeasy MinElute Cleanup (QIAGEN, Hilden, Germany). PolyA-selected RNAs were obtained using TruSeq Stranded mRNA LT Sample Prep Kit (illumina), fragmented, and transcribed into cDNA library using Invitrogen SuperScript Double-Stranded cDNA Synthesis. We sequenced the library to a depth of 20 million read-pairs with 2x75 bp using illumina MiSeq paired-end sequencing (Illumina Inc., San Diego, CA, USA) at the IMB Genomic Core in Academia Sinica (Taiwan).
identifiers.namespace: SUB1592248
identifiers.id: R2D