alias: GSM2889098
title: GSM2889098: control pH6.8 rep3; Rhizobium tropici; RNA-Seq
library_construction_protocol: Total RNA was prepared using the RNeasy mini kit (QIAGEN, Hildesheim, Germany) with some modifications. Pellets were resuspended in RLT buffer, with lysozyme (20 mg per mL; QIAGEN, Hildesheim, Germany) containing Zirconium Oxide Beads (0.5 mm, Next Advance). Cells were disrupted using the Bullet Blender Tissue Homogenizer (Next-Advance) in impact-resistant 2 mL tubes. Genomic DNA was digested with RNase free DNase (QIAGEN, Hildesheim, Germany) for 20 minutes at room temperature. Final RNA concentrations were determined using a NanoDrop (Thermo Scientific). The typical OD260 to OD280 ratio of RNA samples was approximately 2.0. The integrity of RNA samples was verified using a TapeStation 2200 instrument (Agilent Technologies) and the RNA integrity number (RINe) was determined RNA-Seq libraries were prepared using the TruSeq RNA sample Prep kit (Illumina) and sequenced (150 nt per read) by HiSeq 2500 instrument (Illumina) at the Beijing Genomics Institute (BGI, China)
identifiers.namespace: GEO
identifiers.id: GSM2889098