alias: GSM3681584
title: GSM3681584: 2h_Mature_triptolide_repliATAC_rep3; Mus musculus; ATAC-seq
library_construction_protocol: After the appropriate treatment, cells were trypsinized and collected in media. Samples were then processed and tagmented using TD buffer and TDE1 enzyme (Illumina). EdU-positive samples were biotinylated via Click-IT (Invitrogen) and purified with Ampure XP beads (Beckman Coulter). Biotinylated products were captured using MyOne Streptavidin T1 beads (Thermo Fisher Scientific), and the unbound fraction in the supernatant from streptavidin pulldown was purified using the MinElute Reaction Cleanup kit (Qiagen). Steady state samples were purified following tagmentation using the MinElute PCR Purification Kit (Qiagen). Samples were amplified using NEBNext High-Fidelity 2x PCR Master Mix (NEB) and final libraries were purified using Ampure XP beads.
identifiers.namespace: GEO
identifiers.id: GSM3681584