alias: GSM3304659
title: GSM3304659: scRNA-seq_pGBM2; Homo sapiens; RNA-Seq
library_construction_protocol: Xenograft tumor tissue was dissociated into single cells using the following protocol: 10 mL of ACCUTASETM (catalog # 07920) was added to tumor tissue for manual dissociation with surgical scissors. The sample was then incubated with sterile glass beads at 37°C, rotating continuously for 30 min. The glass beads were removed, and the sample was spun at 1000 rpm for 5 minutes at 25°C. The cell pellet was resuspended in neural stem cell media (NeuroCultTM NS-A Proliferation Kit (Human), catalog # 05751). Mouse cells were removed with the Miltenyi Biotec Mouse Cell Depletion Kit (catalog # 130-104-694) and the autoMACSâ Pro Separator and columns (catalog # 130-021-101). Protocol was followed as per manufacturers' instructions. Viability and concentration of the purified human cells were quantified. 10xGenomics scRNA-seq with Chromium console scRNA-seq
identifiers.namespace: GEO
identifiers.id: GSM3304659