alias: E-MTAB-5887:ChIP_Cnot1_LKO_liver_4_p
title: Illumina HiSeq 2500 paired end sequencing; ChIP-seq of livers in control and Cnot1 tamoxifen-inducible liver-specific knockout mice
design: ChIP-seq of livers in control and Cnot1 tamoxifen-inducible liver-specific knockout mice
library_construction_protocol: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.). Control (Cnot1fl/fl) and Cnot1 LKO mice placed on tamoxifen-containing food at 6 weeks of age for 2 weeks. Lysate from liver was subjected to ChIP assay with Histone H3 (tri methyl K4) (H3K4me3) antibody (ab8580; abcam) using SimpleChIP Enzyatic Chromatin IP Kit (#9003; Cell Signaling Technology) with modification. Briefly, after micrococcal nuclease treatment, cross-linked chromatin was incubated with anti-H3K4me3 antibody at 4ºC for 24 hr. The immune complex was washed, and DNA was eluted and de-cross-linked. Libraries for DNA sequence were prepared from DNA isolated by ChIP assays with a KAPA Hyper Prep Kit (Illumina), according to the manufacturer's protocol.
identifiers.namespace: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
identifiers.id: E-MTAB-5887:ChIP_Cnot1_LKO_liver_4_p