alias: GSM2466413
title: GSM2466413: ATRA_ATAC-seq_rep1; Homo sapiens; ATAC-seq
library_construction_protocol: samples were lysed in lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2 and NP-40) for 10 min on ice to prepare the nuclei. Immediately after lysis, nuclei were spun to remove the supernatant. Nuclei were then incubated with the Tn5 transposome and tagmentation buffer at 37 °C for 30 min (Vazyme, China). After the tagmentation, the stop buffer was added directly into the reaction to end the tagmentation. PCR was performed to amplify the library for 15 cycles. After the PCR reaction, libraries were purified with the 1.2× AMPure beads (Beckman, Germany). In house protocol which is similar as NEB library constructions (#7370)
identifiers.namespace: GEO
identifiers.id: GSM2466413