alias: E-MTAB-8905:TZIKA202_p
title: Illumina HiSeq 1500 paired end sequencing; Using next generation sequencing to study the genetic diversity of candidate live attenuated Zika vaccines
design: Using next generation sequencing to study the genetic diversity of candidate live attenuated Zika vaccines
library_construction_protocol: ZIKV RNA was transfected into Vero cells, incubated for nine days, harvested, and clarified by centrifugation to generate passage 0 (P0) ZIKV infectious clones.  Subsequently, P0 ZIKVs were blind-passaged into fresh cultures to generate P1, then serially through P5.  P1-P5 stocks were harvested from cell media at 4-5 days post-infection.  RNA from the Zika virus isolates were extracted using QIAamp Viral RNA mini kit (Qiagen), following the manufacturer's guidelines. NGS libraries were constructed using random TruSeq RNA v2 kit as recommended by the manufacturer.
identifiers.namespace: Department of Microbiology and Immunology University of Texas Medical Branch
identifiers.id: E-MTAB-8905:TZIKA202_p