alias: GSM2121186
title: GSM2121186: mRNA_Ezh2KO1; Mus musculus; RNA-Seq
library_construction_protocol: We abstracted mRNA by using the Rneasy mini kit (Qiagen). For genomic DNA, we use Qiaquick Mini kit (Qiagen) For ChIPSeq, tissues were cross-linked in 1% formaldehyde. The chromatin was sonicated to 250 bp fragments. Histone-DNA complexes were isolated with H3K27me3 antibody (Millipore). Purified DNA was processed according to manufacturer's protocol. For polyA RNA-Seq, Illumina protocol was followed.
identifiers.namespace: GEO
identifiers.id: GSM2121186