alias: GSM2989186
title: GSM2989186: PL026; Mus musculus; RNA-Seq
library_construction_protocol: The sorted mammary basal or luminal cells re-suspended in FACS buffer (300-500 cells/µL) were mixed (3:2 ratio) with C1 Cell Suspension Reagent (Fluidigm) before loading onto a 5 to 10 or 10 to 17-μm-diameter C1 Integrated Fluidic Circuit (IFC; Fluidigm). Each capture site was carefully examined and recorded under a ZEISS microscope in bright field channel for cell doublets. Cell lysing, reverse transcription and cDNA amplification were performed on the C1 Single-Cell Auto Prep IFC, as specified by the manufacturer (protocol 100-7168 E1). The SMARTer Ultra Low RNA Kit (Clontech) was used for cDNA synthesis from the single cells. Illumina NGS library was constructed with Nextera XT DNA Sample Prep kit (Illumina), according to the manufacturer's recommendations (protocol 100-7168 E1).
identifiers.namespace: GEO
identifiers.id: GSM2989186