alias: E-GEUV-2:NA11893.7.MI_120224_2
title: small RNA-sequencing of 465 lymphoblastoid cell lines from the 1000 Genomes
design: small RNA-sequencing of 465 lymphoblastoid cell lines from the 1000 Genomes
library_construction_protocol: Total RNA was extracted from cell pellets using the TRIzol Reagent (Ambion). The pellets had been frozen at ECACC without any additives like RNAlater or Trizol. In Geneva they were thawed, 1mL of trizol was added in each sample, and the samples were transferred to eppendorf tubes. The rest of the protocol followed the manufacturer's guidelines. No DNase treatment was done to the RNA samples. The RNA quality was assessed by Agilent Bioanalyzer RNA 6000 Nano Kit according to the manufacturer's instructions. The sample information includes a visual inspection of RNA quality (degradation), in the scale 0, 0.5, 1. RNA quantity was measured by Qubit 2.0 (Invitrogen) using the RNA Broad range kit according to the manufacturer's instructions. Sample processing for sequencing was done in a random order in each of the seven laboratories. 5 samples were sequenced in every laboratory. Library preparation was done with TruSeq Sm RNA Sample Prep.
identifiers.namespace: Geuvadis
identifiers.id: E-GEUV-2:NA11893.7.MI_120224_2