alias: GSM4544631
title: GSM4544631: Ctrl-PolII-S5; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 20 min and quenched for 10 min in 125 mM glycine. Cells were sonicated in 1% SDS containing sonication buffer for extracting sonicated chromatin Library was constructed using KAPA HyperPrep Kit (Roche 07962363001-KAPA KK8504 ). End Repair/A-tailing step then ligation of IDT for Illumina TruSeq UD Indexes (Illumina-20022370) followed by dual SPRI size selection Left  1.0 - 0.52 Right and 11 cycles of PCR enrichment.
identifiers.namespace: GEO
identifiers.id: GSM4544631