alias: GSM2465274
title: GSM2465274: nos.Luc Total mRNA Biological Repeat 2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol Reagent (Ambion) from 10 pairs of manually dissected ovaries and stored at -80°C until use. Two biological repeats of nos.Luc (Control) and Hop GLKD were used. RNA integrity was verified on an Agilent 2200 TapeStation (Agilent Technologies, Palo Alto, CA). 100-200 ng of total RNA was used to prepare RNA-Seq libraries using the TruSeq RNA Sample Prep kit following the protocol described by the manufacturer (Illumina, San Diego, CA). Please note that polyA containing RNA was first purfied prior to the library preparation step. Single end sequencing (1X50 cycles) was performed on an Illumina HiSeq2500. Sequencing was performed at Hollings Cancer Center Genomics Core Lab at MUSC. RNA libraries were prepared using TruSeq RNA Sample Prep Kit after selecting for polyA RNA
identifiers.namespace: GEO
identifiers.id: GSM2465274