alias: GSM2211716
title: GSM2211716: W0h_rep1; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: Total RNA was isolated from seedlings grown under the conditions listed above, and were isolated by using PureLink Plant RNA Reagent (Invitrogen), following manufacturer's instructions. Small RNAs sized between 17 - 30 bp were resolved by TBE-UREA gel and were sliced for cDNA library construction. RNA libraries were prepared for sequencing using standard Illumina protocols. Tru-seq small RNA for sRNA sequencing replicate 1 and 2. sm ll RNA v1.5 for replicate 3. Degradome library construction has followed the protocol puclished in Methods. 2014 1;67(1):84-90.
identifiers.namespace: GEO
identifiers.id: GSM2211716