alias: GSM3377671
title: GSM3377671: D25 MAFB:GATA3 reporter organoid; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were harvested by pooling 3 organoids, cutting into small pieces with a scalpel blade and dissociating in 1mL Accutase at 37 degrees Celcius, pipetting every 3 minutes. The incubation was stopped after 15 minutes by placing on ice. The cell solution was passed through a 40um cell strainer into a FACS tube and then washed with 2mL E6 (room temperature). FACS tubes were spun (1500 rpm, 3 minutes) and resuspended in 500uL of E6 media. Samples were cell counted and tested for viability and diluted to the required density for the sequencing protocol (300-1000 cells per uL) in E6 media. The cells were then transported to the sequencing facility on ice (30 minutes total). Chromium Single Cell Library kit V2 Illumina HiSeq HT chemistry- 100bp paired end sequencing
identifiers.namespace: GEO
identifiers.id: GSM3377671