alias: GSM2047019
title: GSM2047019: at-endosperm-BS_replicate2_col_x_ler; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Genomic DNA of INTACT-purified endosperm nuclei from 150-300 mg siliques was purified using MagJET Plant Genomic DNA kit (Thermo Fisher Scientific) and sonicated for 30 cycles (30 sec ON, 30 sec OFF at high power) using a Bioruptor (Diagenode). The bisulfite conversion was done using the EpiTect Fast DNA Bisulfite Kit (Qiagen, city, country) during the library preparation. Bisulfite libraries were generated using Ovation Ultralow Methyl-Seq Library Systems (NuGEN) following the manufacturer's protocol using 10 g of starting DNA.
identifiers.namespace: GEO
identifiers.id: GSM2047019