alias: H5S5HS4_region 1
title: WAS gene therapy integration site reconstitution
design: Autologous CD34+ HSCs were transduced with WASP-expressing lentiviral vector and infused into respective patients. Viral integration sites were then sequenced and tracked at various intervals past the infusion.
library_construction_protocol: Amplicon library was constructed using the protocol described in Wang et.al NAR 2008. In brief, genomic DNA is digested/fragmented for each sample and ligated with linkers and then amplified by nested PCR.
identifiers.namespace: UPENNBL
identifiers.id: H5S5HS4_region 1