alias: GSM3679741
title: GSM3679741: Islets-MIP-GFP- GFP+ D; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted into lysis buffer an messanger RNA was captured with 15ul of Dynabeads oligo(dT) (Life technologies), and washed according to manufacture guidelines. Purified messanger RNA was eluted at 70C with 6ul of 10mM Tris-Cl pH 7.5 and stored at -80C.For library preparation RNA was used for the bulk MARS-seq  protocol (Jaitin et al. 2014 Science.343:776-9). MARS-seq libraries were sequenced using Illumina NextSeq 500 High Output v2 Kit (75 cycles).
identifiers.namespace: GEO
identifiers.id: GSM3679741