alias: GSM2808236
title: GSM2808236: mLEC_EPZ5676_k79me2; Mus musculus; ChIP-Seq
library_construction_protocol: The primary mouse dermal LECs derived from C57BL/6 were were maintained in CellBiologics culture medium supplemented with VEGF, ECGS, Heparin, EGF, Hydrocortisone, L-glutamine, Antbiotic-Antimycotic solution, FBS). Approximately 1-3 x 10^7 cells were used for each ChIP-Seq assay. The chromatin was precipitated by H3K79me2 antibody (ab3594, Abcam) and immunoprecipitated DNAs were purified with treatment of proteinase K, phenol/chloroform and ethanol precipitation. ChIP-Seq libraries were produced using Truseq ChIP Sample kit (Illumina) according to manufacturer's instruction.
identifiers.namespace: GEO
identifiers.id: GSM2808236