alias: Library1_Tplus_2
title: "TEX and TAP treated RNA from Methanocaldococcus jannaschii: library 1, replicate 2."
design: "RNA samples isolated from M. jannaschii cell pellet and treated with Terminator exonuclease (TEX) then split in two with one half being treated with tobacco acid pyrophopatase (TAP, Tplus, untreated = Tminus). cDNA was prepared and further fragmented by ultrasound and libraries produced which included size fractionation to 220-600bp (including sequencing adaptors). Sequences were alingned to M. jannaschii genome with default parameters except -n 0 -m 1 -5 2 (to trim off extra nuclotides added to improve efficiency of adapter ligation). "
identifiers.namespace: University College London
identifiers.id: Library1_Tplus_2