alias: GSM1672251
title: GSM1672251: WS; Gallus gallus; RNA-Seq
library_construction_protocol: Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample RNA libraries were prepared for sequencing using standard Illumina protocols. Three RNA samples from either the black or white skin samples were pooled following mRNA isolation. The isolated mRNA was fragmented, followed by first-strand cDNA synthesis using random hexamer primers. The second-strand cDNA was synthesized using buffer ( Invitrogen, 20 µL ), dNTPs ( 0.25 mM / µL ), RNaseH ( 0.05 U / µL ) and DNA polymerase I ( 0.5 U / µL ). The short cDNA fragments were purified using the QIAQuick PCR extraction kit ( LianChuan Sciences, Hangzhou, China). The fragment ends were repaired, A-tailed and ligated to sequencing adaptors. Suitably sized ( 350 ± 50 bp ) fragments were selected following an agarose gel electrophoresis and used as templates for the PCR amplification to generate an RNA-seq library. The sequencing of the library was performed using an Illumina HiSeq 2000 ( LianChuan Sciences, Hangzhou, China).
identifiers.namespace: GEO
identifiers.id: GSM1672251