alias: 113
title: 16S rRNA sequencing of human fecal donor samples
design: 16S rRNA gene sequencing was performed at the University of Nebraska Medical Center Genomics Core Facility. DNA normalization and library prep were performed followed by V3-V4 16S rRNA amplicon gene sequencing using a MiSeqV2 (Illumina) The following primer sequences were used: (Primer sequences: Forward Primer = 5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG 16S Amplicon PCR Reverse Primer = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCCIllumina overhangs: Forward overhang: 5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG[locusspecific sequence] Reverse overhang: 5 GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG[locusspecific sequence]).
identifiers.namespace: SUB7149503
identifiers.id: 113