alias: GSM2318624
title: GSM2318624: KO_input_s22; Mus musculus; ChIP-Seq
library_construction_protocol: IECs were isolated with a modified method (Goodyear AW, et al. J Immunol Methods 2014;405:97-108). Dissociated IECs were collected by centrifugation. IECs were freshly isolated from both DIECUN and F/FUN neonates at P12 and were subjected to ChIP (MAGnify ChIP system, ThermoFisher). After reverse cross-linking, library preparation was carried out by using the MicroPlex Library Preparation Kit V2 (Diagenode, C05010012). Cluster generation and 50 cycles of single end sequencing were carried out on an Illumina Nexseq 500. Sequence tags were aligned (mapped) to the unmasked mouse (mm9) or human (hg19) reference genome.
identifiers.namespace: GEO
identifiers.id: GSM2318624