alias: GSM3302917
title: GSM3302917: preSC-shCrebbp-5; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in TRIzol reagent with RNA isolated following manufacturers instructions. RNA-seq libraries were prepared using theNEBNext Ultra RNA Library Prep Kit for Illumina (catalog E7530L). Briefly, 500 ng total RNA was used to isolate mRNA with the NEB Next Oligo dT Magnetic Isolation Module (catalog E7490L). The isolated mRNA undergoes fragmentation, priming, cDNA synthesis and end repair/dA-tail. Individual index primers for multiplexing were added during PCR library enrichment after adaptor ligation.
identifiers.namespace: GEO
identifiers.id: GSM3302917