alias: GSM2889560
title: GSM2889560: Fibroblast; Sarcophilus harrisii; RNA-Seq
library_construction_protocol: For expression analysis from cell lines, total RNA was isolated from approximately 1 x 106 cells using QIAzol lysis reagent according to the manufacturer's instructions (Qiagen). RNA-seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). The 5 libraries were pooled, diluted and sequenced on Illumina HiSeq 3000/4000 using 75 bp paired-end chemistry.
identifiers.namespace: GEO
identifiers.id: GSM2889560