alias: GSM2122536
title: GSM2122536: Mot1 input; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Cells were lysed, sonicated, and clarified by centrifugation.  Lysates were incubated with antibody for either myc-tagged protein or histone H3.  Immunoprecipitated samples were washed twice each with lysis buffer (140 mM NaCl), lysis buffer (500 mM NaCl), LiCl wash buffer, and 1X TE.  DNA was eluted from beads in ChIP elution buffer and cross-links were reversed overnight.  DNA was then purified using Qiagen PCR purification kit. Libraries were constructed using TruSeq ChIP Library Prep Kit from Illumina (IP-202-1012) according to manufacturer's instructions and are briefly described here.  Purified DNA was end repaired, and 3' ends were adenylated.  Adapters were ligated to adenylated ends and products were gel purified.  DNA with adapters was PCR enriched, quantified via Qubit, and validated using Bioanalyer.
identifiers.namespace: GEO
identifiers.id: GSM2122536