alias: GSM4663519
title: GSM4663519: 1927_ACC_AP; Homo sapiens; ATAC-seq
library_construction_protocol: 100 mg of pulverized anterior cingulate cortex (ACC) tissue was obtained from the NIMH Human Brain Collection Core (HBCC). Total nuclei were  extracted from frozen brain tissue using sucrose gradient centrifugation. Neuronal (NeuN+) and non-neuronal nuclei (NeuN-) were isolated with fluorescence-activated nuclei sorting  using an anti-NeuN antibody conjugated to AlexaFluor-488 on a FACSAria instrument. For a detailed protocol for nuclei isolation, see Kundakovic et al, 2017 ATAC-seq libraries were prepared according to Buenrostro et al. 2013 (PMID:24097267) with some modifications. Illumina Nextera DNA Library Prep kit (FC-121-1030) and Nextera Index kit (FC 121-1011) were used to prepare the libraries.
identifiers.namespace: GEO
identifiers.id: GSM4663519