alias: GSM3679903
title: GSM3679903: Foxp3-GFP+Treg_cKO_Rep3_scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspensions were purified by total CD4+ T cell enrichment followed by FACS-sorting stored in 40 µl RNAlater™ Stabilization Solution at -80 °C Samples were processed using the QIAshredder Kit (Qiagen) according to the manufacturer's protocol RNA was extracted from samples using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocol RNA libraries were prepared for single-cell RNA sequencing (scRNA-seq) using the Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10x Genomics), processed with the Chromium (10x Genomics) and sequenced using the HiSeq 4000 System (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM3679903