alias: GSM4412481
title: GSM4412481: A00711: patient with aortic dissection2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from the tissue was extracted using TRIzol reagent (Invitrogen Corporation, CA, USA) in accordance with the manufacturer's instructions. The RNA was then fragmented chemically into about 100 nucleotides in length using fragmentation buffer (Illumina, Inc.). The RNA was precipitated using the aqueous phase by mixing with isopropyl alcohol. The samples were incubated at 15ºC to 30℃ for 10 minutes and centrifuged at 12,000 x g for 10 minutes at 4℃. The ribosomal RNA-depleted RNAs were then used to construct RNA libraries with TruSeq Stranded Total RNA Library Prep kit (Illumina, CA, USA). The strand-specific library construction uses dUTP instead of dTTP to label the second-strand cDNA during synthesis. Uracil-DNA glycosylase (UDG) was used to hydrolyze the strand before polymerase chain reaction (PCR) enrichment to ensure that the final sequencing data was from the first cDNA strand.
identifiers.namespace: GEO
identifiers.id: GSM4412481