alias: GSM3678734
title: GSM3678734: HC21: 11 Cingulate Anterior (BA24); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol reagent (Invitrogen, Carlsbad, California). Sequencing libraries were prepared with TruSeq RNA Sample Preparation Kit (Illumina) according to manufacturer's instruction. Briefly, poly-T oligo-attached magnetic beads were used to isolate long polyadenylated RNA from 1 μg of total RNA. After fragmentation, first-strand cDNA was reverse transcribed with random hexamer-primers, followed by second-strand cDNA synthesis, end repair, adenylation of 3′ ends, and ligation of the adapters. Fragments were then enriched by PCR.
identifiers.namespace: GEO
identifiers.id: GSM3678734