alias: GSM3680131
title: GSM3680131: 1_E_AS_06; Mus musculus; RNA-Seq
library_construction_protocol: The medial prefrontal cortex was dissected, minced, and resuspended in HBSS. 5% of the suspension was removed and the remaining cells were manually dissociated in Neural Tissue Dissociation Buffer (P) (Miltenyi Biotec). Myelin was removed using a 25% Percoll gradient and astrocytes were captured using ACSA2 microbeads (Miltenyi Biotec). Cells were spun at 1,000 x g and flash frozen in liquid nitrogen.  Tissue isolation described in samples. RNA was harvested using Qiagen Rneasy Micro Kit (Cat No. 74004). Samples were Poly(A) selected using the Poly(A) Purist Kit (Thermo Fisher Scientific Inc.) and prepared using NEBNext® Ultra™ Directional RNA Library Preparation Kit (New England BioLabs, Ipswich, MA). Samples went through 15 cycles of PCR. Samples were sequenced on the HiSeq 4000 (Illumina, San Diego, CA) using (150 bp; paired-end reads) to a minimum target depth of 20 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM3680131