alias: GSM3473818
title: GSM3473818: iron_2; Malus domestica; RNA-Seq
library_construction_protocol: Total RNA was extracted from root samples of control plants and treated plants using the Spectrum™ Plant Total RNA Kit (Sigma Aldrich). cDNA library preparation and sequencing reactions were performed by IGA Technology Services s.r.l. (Udine, Italy). An amount of 2 μg of total RNA was used for library preparation following the Illumina protocol TruSeq 2.0. Briefly, RNA was fragmented into fragment with an average of 500 bp. Then, mRNA was purified using poly-T beads. The first- and second-strand cDNAs were synthesized and end repaired. Adaptors were ligated after adenylation at the 3' ends and cDNA templates were enriched by PCR. The 50 bp single end reads were obtained using an Illumina Hiseq2000 platform.
identifiers.namespace: GEO
identifiers.id: GSM3473818