alias: GSM4417086
title: GSM4417086: BRAC861.2e_MEMO [WT.BCELLS.1]; Mus musculus; RNA-Seq
library_construction_protocol: Spleen were disrupted using a 70uM mesh strainer. Samples were then washed with FACS buffer (2% FBS and 2 mM EDTA in PBS), incubated for 30 min on ice  with antibodies and  CD43 MicroBeads (Cat# 130-049-801, Miltenyi Biotec). Red blood cells were then  lysed for 5 min at room temperature with 5mL of Gey's buffer. After a wash , samples were passed through a 70 um filter LS columns (Miltenyi) for magnetic separation and B cell enrichment. After washing,  single-cell suspension was  sorted by flow cytometry. Samples were collected in eppendorffs containing RTL Buffer Plus (Qiagen) with 1% beta-mercaptoethanol. Samples were purified with AllPrep DNA/RNA Mini Kits (Cat# 80204, Qiagen) as per  manufacturer's instructions. Samples were prepared with Nugen cDNA kit and sequenced using Illumina HiSeq system
identifiers.namespace: GEO
identifiers.id: GSM4417086