alias: GSM2808200
title: GSM2808200: Rep1_H2; Ettlia oleoabundans; RNA-Seq
library_construction_protocol: Samples were centrifuged and cell pellets snap frozen in liquid nitrogen and stored at -80ºC.Total RNA was extracted from microalgal cells (~100-200 mg wet weight) by cell disruption by bead-beating in bead-beater tubes (Lysing Matrix E; MP Biomedicals, Santa Ana, CA, USA) for 1x10 sec at 5500 rpm in a Precellys® 24 bead beater (Bertin Technologies), in the presence of RLT buffer with β-mercaptoethanol (Qiagen). Subsequently, tubes were placed on ice and spun down for 2 min at 13.000 x g. RNA was further cleaned using the RNeasy Plant Mini Kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2808200