alias: GSM3831433
title: GSM3831433: IR25_24h_3; Oryza sativa; RNA-Seq
library_construction_protocol: Total RNA of each sample was isolated using TRIzol (Ambion) according to the manufacturer's instructions. For each sample, 25 µg of total RNA was used for circRNA-seq library preparation. Total RNA was depleted for ribosomal RNAs using the RiboMinus kit. The remaining RNAs were iron fragmented at 95℃ followed by end repair and 5' adaptor ligation. Then reverse transcription was performed with RT primer harboring 3' adaptor sequence and randomized hexamer. The cDNAs were purified and subjected to PCR amplification. PCR products corresponding to 300-500 bp were purified and quantified, and then stored at -80 ℃ until used for sequencing
identifiers.namespace: GEO
identifiers.id: GSM3831433