alias: GSM2735496
title: GSM2735496: OM10-XT; Homo sapiens; RNA-Seq
library_construction_protocol: Nextera XT method Exosomes were isolated from culture media using nanotrap particles NT80 and NT82 (Ceres Nanosciences).  The samples were rotated with the nanotraps overnight at 4 degrees Celsius.  The RNA was then isolated from the exosomes using TRI Reagent-LS (MRC) according to the manufacturer's protocol. The extracted RNA was converted to cDNA using miRNA cDNA Synthesis Kit (Applied Biological Materials Inc.) with two sequence specific primers. Three sequencing libraries were made from the same cDNA library: 1) Nextera XT method, 2) Fragmentase method, and 3) unfragmented.
identifiers.namespace: GEO
identifiers.id: GSM2735496