alias: GSM2465742
title: GSM2465742: CD200nCD200R1p mouse 3; Mus musculus; RNA-Seq
library_construction_protocol: Mouse mammary cells were dissociated from the fourth inguinal mammary glands. Lin- cell suspension was prepared using the EasySep mouse mammary enrichment kit (StemCell Technologies, Vancouver, Canada).  Epithelial cells were resuspended in HF containing Hank's balanced salt solution (HBSS, Biological Industries) supplemented with 0.1% (v/v) Hepes (Biological Industries) and 2% (v/v) fetal bovine serum (FBS, Biological Industries) at a concentration of 107 cell/ml and incubated for 90 min on ice with fluorescent conjugated antibodies. Cell viability was tested by staining with propidium iodide (PI) (Sigma St. Louis, MO) or BD Horizon Fixable Viability Stain 450 (FVS450, BD Biosciences) according to the manufacturers' protocol. Dispersed mouse mammary cells were sorted using FACSAria II. 1000 cells 9.5µl of RNAsin-containing lysis buffer, incubated for 5 min at room temperature and kept at -80°C. SMART-Seq V4 Ultra Low Input RNA kit
identifiers.namespace: GEO
identifiers.id: GSM2465742