alias: GSM1674728
title: GSM1674728: PAO1 ∆anr replicate 2; Pseudomonas aeruginosa PAO1; RNA-Seq
library_construction_protocol: Colony biofilms of PAO1 and J215 wild type or Δanr were grown for 12 h, then harvested in 1 mL of PBS applied to the plate followed by recovery with an angled glass rod. Samples were pelleted by centrifugation and stored at -80°C. RNA was isolated from pelleted cells using the RNeasy mini kit (Qiagen), followed by treatment with RQ1 DNAase from Promega, both in accordance with manufacturers' instructions. Ribosomal and transfer RNAs were removed using the MICROBExpress kit (Life Technologies) according to manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM1674728