alias: E-MTAB-7607:NG-7876_MG_E_II_lib63510_3350_3_1.fastq.bz2_s
title: Illumina HiSeq 1000 sequencing; A comprehensive reference transcriptome for Brachypodium distachyon grain development incorporating key developmental transitions and identifying potential regulators
design: A comprehensive reference transcriptome for Brachypodium distachyon grain development incorporating key developmental transitions and identifying potential regulators
library_construction_protocol: Tissue samples were collected from eight distinct developmental stages; pre-anthesis ovaries, young grains (1-3 DAA), middle length grains (3-8 DAA), full length grains (8-15 DAA), mature grains (15-20 DAA), mature grains without embryo, germinating grains and seedlings at 3-4 days after germination (Fig.1). Tissues were frozen in liquid nitrogen immediately after collection and were kept at -80°C until further analyses. Brachypodium distachyon grains (Bd-21 accession) were germinated on moist filter paper after stratification at 4°C for 48h. Five-day old seedlings were transferred to 9cm square pots with a 2:2:1 multipurpose compost: vermiculite: sand mix and grown under controlled environment conditions with a 18h photoperiod at 20°-22°C and light intensity of 180-200 umol/m/s. Total RNA was isolated from frozen tissue samples using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer's instructions. Three biological replicates were used for each stage of development. Random primed cDNA libraries were created and sequenced by GATC Biotech (https://www.gatc-biotech.com).
identifiers.namespace: Associate Professor Department of Genetics and Genome Biology
identifiers.id: E-MTAB-7607:NG-7876_MG_E_II_lib63510_3350_3_1.fastq.bz2_s