alias: GSM4413052
title: GSM4413052: Day24 pLN_002 CD90hi GCTFH-like; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from sorted cell populations using the Direct-zol RNA Kits (Zymo Research). Prior to cDNA synthesis, the quantification and quality assessment of RNA was performed using a Qbit 4.0 fluorimeter with the RNA IQ Assay (Thermo Fisher Scientific) and Bioanalyzer with Agilent RNA 6000 Pico Kit (Agilent). The cDNA was produced using the SMART-Seq® v4 Ultra Low Input RNA Kit following manufacturer's recommendations (Takara Bio USA Inc.). Adapters were used as template for cDNA synthesis and downstream PCR for the cDNA amplification. The resulting cDNA was purified using the KAPA Pure Beads (Roche Sequencing and Life Science), and quantification and quality assessment was performed using a Qbit 4.0 fluorimeter with the dsDNA HS Assay Kit (Thermo Fisher Scientific). The DNA library was constructed using the KAPA HyperPlus Kit following manufacturer's recommendations (Roche Sequencing and Life Science).
identifiers.namespace: GEO
identifiers.id: GSM4413052