alias: GSM2465979
title: GSM2465979: YW_29C_1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: BEAF32 experiment: RNA was extracted from their heads using TRI Reagent, Sigma Aldrich. Full length RNA-seq and 3’-seq using 100bp: RNA was extracted using Trizol (Invitrogen). BEAF32 experiment: (Dnase treated) RNA was fragmented in the presence of 10XFastAP buffer (ThermoFisher Scientific), at 94°C for 3 minutes, and placed on ice. RNA was then dephosphorylated with FastAP, cleaned (2X SPRI) and ligated to linker1. Library preparation was continued as described for Exo-seq, starting from linker1 3’ ligation. Full length RNA-seq: Full-length RNA libraries were done similarly to the described 'RNAseH- samples', with a difference in the order of the different steps; First, RNA was PolyA+ selected, then fragmented (using Mg-based fragmentation), cleaned (2.5X SPRI cleanup), FastAP treated, cleaned (Silane cleanup) and continued in library preparation (starting at first ligation of 3’ adapter). 3’-seq using 100bp: Library preparation was done as described for RNaseH- samples.
identifiers.namespace: GEO
identifiers.id: GSM2465979