alias: GSM2890287
title: GSM2890287: T2_wt_1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was isolated from independently prepared batches of hand-dissected haltere and third-leg imaginal discs (T3 discs) or wing discs (T2 discs) from wild-type or ash122 homozygous larvae, using the Direct-zol RNA mini Prep Kit (Zymo Research). After additional Agencourt AMPure XP purification (Beckman Coulter) isolated RNA was analyzed on a Bioanalyzer (Agilent) using an RNA 6000 Nano Chip (Agilent). Non-degraded RNA from independent biological replicates of each type of sample was used to construct sequencing libraries with the sense mRNA-Seq Library Prep Kit (Lexogen). Quality-controlled and quantified libraries were sequenced on an HiSeq1500 system (Illumina) in the single-end mode (100 nt reading length).
identifiers.namespace: GEO
identifiers.id: GSM2890287