alias: GSM2113041
title: GSM2113041: Warm challenge 2 Input; Mus musculus; RNA-Seq
library_construction_protocol: Detailed protocol and buffer recipes are described previously(Knight et al., 2012). Briefly, mice were rapidly sacrificed by cervical dislocation and brains were removed, rinsed briefly with cold dissection buffer and place in a cold sectioning matrix. 0.5 mm sections were obtained and the anterior hypothalamus was removed under a dissecting microscope. Amplified cDNA was prepared using Ovation RNA-Seq System V2, and sequencing library was prepared using the Ovation Ultralow DR Multiplex system and sequenced on an Illumina HiSeq2500 platform
identifiers.namespace: GEO
identifiers.id: GSM2113041