alias: GSM3303890
title: GSM3303890: Papilloma- Vehicle 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation proceeded using the TRIzol Plus RNA Purification Kit (Thermo Fisher, Cat. #12183-555). Briefly, homogenized epidermis in TRizol was incubated at room temperature for five minutes. 0.2 mL chloroform (Sigma Aldrich, CAS 67-66-3, PN 360927) was added. Lysate was centrifuged in 5 PRIME, phase lock heavy 2 mL tubes (Cat. # 2303830). An equal volume of ethanol was added (Decon, CAS 64-17-5). DNAse treatment using RNAse-Free DNAse was performed (Qiagen Cat. #79254). RNA was eluted using DEPC Treated Water (Invitrogen, Part no. 46-2224). A series of washing steps was conducted following the directions provided in the TRIzol Plus RNA Purification Kit. The purity of RNA was confirmed using a Bioanalyzer (Instrument Name DE72901373, Firmware C.01.069, Type G2939A. Three samples from each treatment with the best RNA integrity were chosen for RNA sequencing. Poly-A pull-down was used to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required). Library preparation was performed by using Illumina TruSeq RNA prep kit according to manufacturer protocols.
identifiers.namespace: GEO
identifiers.id: GSM3303890