alias: GSM2049177
title: GSM2049177: A2780 exosome; Homo sapiens; miRNA-Seq
library_construction_protocol: Exosomes were isolated from the conditioned medium by differential ultracentrifugation. Total RNAs were extracted from purified exosomes using the RNAqueous-Micro kit (Thermo Fisher Scientific), and the amount and quality of small RNA in the total RNA samples were determined using the Agilent small RNA kit on an Agilent 2100 Bioanalyzer. Five nanograms of small RNAs were subjected to library construction. Library was constructed using the Ion Total RNA-Seq kit v2 (Life Technologies Corp.), according to the small RNA protocol.
identifiers.namespace: GEO
identifiers.id: GSM2049177