alias: GSM2051458
title: GSM2051458: ASF00616_WT; Mus musculus; RNA-Seq
library_construction_protocol: Pancreas was lysated in Buffer GC and RNA isolated using Pheno-Chloroform Total RNA (1µg) was spiked with ERCC ExFold RNA spike-In mixes (Life Technologies). RNA quality was assessed on an Agilent 2100 Bioanalyzer and samples with a RNA Integrity Number > 8.5 were used. PolyA+ fractions were purified, randomly fragmented, converted to double stranded cDNA, and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters following Illumina's "TruSeq Stranded mRNA Sample Preparation Part # 15031047 Rev. D" (this kit incorporates dUTP during 2nd strand cDNA synthesis, which implies that only the cDNA strand generated during 1st strand synthesis is eventually sequenced). Adapter-ligated libraries were generated by PCR with Illumina PE primers (8 cycles). The resulting purified cDNA libraries were applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx with SBS TruSeq v5 reagents by following manufacturer's protocols.
identifiers.namespace: GEO
identifiers.id: GSM2051458