alias: GSM2809100
title: GSM2809100: WT-1 input-seq; Mus musculus; RNA-Seq
library_construction_protocol: mRNAs were isolated from total RNAs of NSPCs and fragmented into around 100nt length with sonication at 10 ng/ul in 100 ul RNase-free water with Bioruptor Pico (Diagenode) with 30 cycles of 30 sec on followed by 30 sec off. libraries were prepared with Truseq Stranded mRNA library Prep Kit with the RFP incubation step shorten from 8 min to 20 sec.
identifiers.namespace: GEO
identifiers.id: GSM2809100