alias: GSM4544735
title: GSM4544735: 20Z-pXyl-methane-Rep2; Methylomicrobium alcaliphilum 20Z; RNA-Seq
library_construction_protocol: Total RNA was stabilized with the bacterial reagent RNAprotect (Qiagen, Hilden, Germany) and then extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany) along with On-Column DNase Digestion using RNAse-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer's protocols. Total RNA quality and quantity were measured using an RNA 6000 Nano kit with the Agilent Bioanalyzer (Agilent Technologies, CA, USA). Then rRNA was removed using Ribo-Zero rRNA removal kits (Gram-negative bacteria; Epicentre, Madison, WI, USA) and then RNA was used for sequencing library using TruSeq Stranded Total RNA Sample Prep Kit (Illumina, USA) according to the manufacturer's instructions. Briefly, transcriptome sequencing was conducted using Illumina/Hiseq-2000 RNA sequencing (Macrogen, Korea).
identifiers.namespace: GEO
identifiers.id: GSM4544735