alias: E-MTAB-5325:Sample 4_s
title: Illumina HiSeq 2500 sequencing; RNA-seq of mouse pancreatic pericytes from wild-type and Tcf7l2-mutated mice
design: RNA-seq of mouse pancreatic pericytes from wild-type and Tcf7l2-mutated mice
library_construction_protocol: wild-type mice and Nkx3.2-Cre;Tcf7l2 f/f mice were generated and their pancreatic tissues dissected at 10 weeks of age For cell isolation, dissected pancreatic tissues were digested with Collagenase P (0.4 mg/ml; Roche) and DNase (0.1 ng/ml; Sigma) diluted in HBSS for 30 minutes at 37 ̊C to obtain single cell. DAPI (1 l/ml; Sigma-Aldrich) was added to label dead cells. Live fluorescently-labeled cells were collected using FACS Aria (BD). Using PureLink RNA micro kit  RNA was amplified using SMART-Seq v4 Ultra low Input RNA kit for Sequencing (Clontech), followed by Illumina sequencing library preparation
identifiers.namespace: Dept. of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
identifiers.id: E-MTAB-5325:Sample 4_s