alias: GSM2466428
title: GSM2466428: control_Hi-C_rep2; Homo sapiens; OTHER
library_construction_protocol: BL-Hi-C:cells are cross-linked with formaldehyde and nucleuses are isolated by lysis buffers. The chromain is then randomly fragmented by 4-bp enzymes (HaeIII, MboI or HindIII) and then two proximal fragments are connected by 20 bp biotinylated bridge linker in nucleus. A whole genome chromatin is extracted and sheared into 400 bp. Then the biotinylated junctions are captured by M-280 streptavidin magnetic beads. After amplification, a BL-Hi-C library is sequenced by illumina sequencer with paired-end strategy. In house protocol which is similar as NEB library constructions (#7370)
identifiers.namespace: GEO
identifiers.id: GSM2466428