alias: GSM2808419
title: GSM2808419: Lats1/2iΔLgr5 2; Mus musculus; RNA-Seq
library_construction_protocol: Mouse stomachs were harvested, cut open, and pinned flat on a thick sheet of paper. They were snap-frozen in vapor-phase liquid nitrogen, and mounted with frozen sectioning media (Leica). The samples were then sectioned in a cryostat, with five to seven 10 μm sections collected beginning with the first observed epithelial structures. RNA was isolated from each set of samples using easy-BLUE (Intron). For library construction, we used Truseq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly, the steps of the strand-specific protocol are as follows: first strand cDNA synthesis; second strand synthesis using dUTPs instead of dTTPs; end repair, A-tailing and adaptor ligation; PCR amplification. Then, each library was diluted to 8 pM for 76 cycles of paired-read sequencing (2 X 75 bp) on an Illumina NextSeq 500 following the manufacturer's recommended protocol.
identifiers.namespace: GEO
identifiers.id: GSM2808419