alias: E-MTAB-5901:Cnot1_control_liver_ActD_4h_2_p
title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of livers in control and Cnot1 tamoxifen-inducible liver-specific knockout mice
design: RNA-seq of livers in control and Cnot1 tamoxifen-inducible liver-specific knockout mice
library_construction_protocol: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.).  For comprehensive mRNA half-life profiling, we injected control (Cnot1 fl/fl) and Cnot1 LKO mice, which were placed on a tamoxifen-containing diet at 6 weeks of age for 2 weeks, intraperitoneally with 2 mg actinomycin D per g body weight for 4 and 8 hr, and then liver samples were collected. Total RNA was isolated from liver using Isogen II (Nippongene), according to the manufacturer's protocol 1 µg of total RNA was used for RNA-seq library preparation with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina), according to the manufacturer's protocol.
identifiers.namespace: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
identifiers.id: E-MTAB-5901:Cnot1_control_liver_ActD_4h_2_p