alias: GSM3680753
title: GSM3680753: 122-E (miRNA-Seq); Ovis aries; miRNA-Seq
library_construction_protocol: A sample from encephalon (parietal lobe cortex) was aseptically taken from each animal and tissue sections were preserved in RNAlater solution (Ambion, Austin, TX, USA) at −80 ⁰C. Total RNA was isolated from encephalon tissue using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) and PureLink RNA Mini Kit (Invitrogen). 60 mg tissue samples were homogenized in 1 ml of TRIzol Reagent using Precellys®24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France) combined with 1.4 and 2.8 mm ceramic beads mix lysing tubes (Bertin Technologies). RNA isolation was performed following manufacturer instructions and RNA was suspended in RNase free water and stored at −80 ⁰C. The TruSeq Small RNA library prep kit (Illumina, San Diego, CA, USA) was used for the construction of sequencing libraries. miRNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM3680753