alias: S10_Upper_Arid_SB_1_Soil_ITS_1
title: Miseq of Opuntia ficus-indica:  Rhizosphere isolated from spineless plant of Sidi Bouzid
design: The ITS 1-2 region PCR primers (ITS1/2) were used in a 28 cycle PCR (5 cycle used on PCR products) using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. After amplification, PCR products are checked in 2% agarose gel to determine the success of amplification and the relative intensity of bands. Multiple samples are pooled together (e.g., 100 samples) in equal proportions based on their molecular weight and DNA concentrations. Pooled samples are purified using calibrated Ampure XP beads. Then the pooled and purified PCR product is used to prepare illumina DNA library. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq following the manufacturers guidelines. Sequence data were processed using MR DNA analysis pipeline (MR DNA, Shallowater, TX, USA).
identifiers.namespace: SUB4960423
identifiers.id: S10_Upper_Arid_SB_1_Soil_ITS_1