alias: GSM4416986
title: GSM4416986: Mϕ THP-1_nuclear_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol. Genomic DNA was extracted using standard phenol-chloroform method. Nanopore direct cDNA sequencing (SQK-DCS109) was performed using the flow cell R9.4 on the MinION machine (Oxford Nanopore Technologies) following the manufacturers' instructions with minor modifications. Briefly, first strand cDNA was made from 100 ng of poly-A-enriched RNA using the VN primer and SuperScript II (Invitrogen) at 42°C for 50 min. After removal of RNA by RNase Cocktail Enzyme Mix (Thermo Fisher), second strand cDNA was made using random hexamers (Invitrogen) and LongAmp Taq Master Mix (New England Biolabs), which was followed by the End-prep and Adapter ligation before subjecting the library to the flow cell.
identifiers.namespace: GEO
identifiers.id: GSM4416986