alias: GSM2318497
title: GSM2318497: RNASEQ_380-1; Trichodesmium erythraeum IMS101; RNA-Seq
library_construction_protocol: Cultures were gently filtered onto 5 µm polycarbonate filters (Whatman) during the middle of the photoperiod, immediately flash frozen, and stored in liquid nitrogen until RNA extraction. RNA was extracted using the Ambion MirVana miRNA Isolation Kit (Thermo Fisher Scientific) in an RNAse free environment according to the manufacturer’s instructions followed by two incubations with Ambion’s Turbo DNA-free kit to degrade trace amounts of DNA. Extracted RNA was sent to the UC San Diego IGM Genomics Center for library construction and Illumina sequencing. Briefly, rRNA was removed from total RNA using the Ribo-Zero rRNA Removal Kit (Illumina), and libraries were constructed with the TruSeq Stranded mRNA Library Prep Kit (Illumina) followed by 50 base pair, single end sequencing with the Illumina HiSeq4000.
identifiers.namespace: GEO
identifiers.id: GSM2318497