alias: GSM3227962
title: GSM3227962: EU5: EdU_seq_aB_28h_HU_28h_0.5mM_rep1; Mus musculus; OTHER
library_construction_protocol: Cells were incubated with 20 uM EdU for 28 hours (B cells, T Cells).  For Click-IT reaction, cell pellets were resuspended in PBS, 10 M Biotin Azide (ThermoFisher Cat# B10184), 200 uM CuSO4 (Sigma), and 10 mM sodium ascorbate (Sigma) for 2 hours, at room temperature,in the dark for 2 hours. Biotin-EdU labeled DNA fragments were puriﬁed using MyOne Streptavidin C1 Beads (ThermoFisher #650-01).  35 L of Dynabeads were washed twice with 1 mL Binding and Wash Buffer (1xBWB) (10 mM Tris-HCl pH8.0, 1 mM EDTA, 1 M NaCl, 0.1% Tween20). Beads were recovered using a DynaMag-2 magnetic separator (12321D, Invitrogen). Supernatants were discarded. libraries were prepared and sequenced following standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM3227962