alias: GSM2808499
title: GSM2808499: ATCC13869; Corynebacterium glutamicum; RNA-Seq
library_construction_protocol: Total RNA was prepared using the Qiagen RNeasy Total RNA kit. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. After agarose gel electrophoresis, the suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeqTM 2000
identifiers.namespace: GEO
identifiers.id: GSM2808499