alias: GSM2211335
title: GSM2211335: Forebrain__Male__WT__rep1; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were harvested and immediately homogenized in TRIzol. Total RNA was isolated according to the manufacturer's protocol (RNeasy Mini, Qiagen). Libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina). For each tissue, 500ng of total RNA was used as input, with the exception of lung (200ng) and pre-somitic mesoderm (80ng). Libraries were individually barcoded, amplified for only 10 PCR cycles to minimize artifacts, pooled into groups of six with a mix of wildtype and knockout samples, and then sequenced on an Illumina HiSeq 2500.
identifiers.namespace: GEO
identifiers.id: GSM2211335