alias: GSM3830030
title: GSM3830030: BORIS_Abs_FT282+BORIS_ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: After overnight incubation of DiaMag magnetic beads (Diagenode, Inc.) with either CTCF or BORIS monoclonal antibodies (characterized and described by PMID:15731119; PMID: 21659515), precipitated chromatin was washed, decrosslinked, and digested with proteinase K. The resulting DNA purified using phenol/chloroform/isoamyl alcohol. DNA concentration  assessed with Quant‐it PicoGreen dsDNA kit (Invitrogen) and 5–10 ng was used to generate sequencing libraries. TruSeq ChIP Sample Preparation Kit (Illumina, Inc., USA). RNA-seq libraries were prepared using either Ion Total RNA-seq v2 kit or TruSeq Stranded RNA LT Kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM3830030