alias: GSM4413102
title: GSM4413102: MEL/ch11_HS3∆∆GA_H3K27ac_ChIP-Seq; Mus musculus; ChIP-Seq
library_construction_protocol: Nuclei were digested with 100 units of MNase at 37°C for 15 min. Mono or di-nucleosomes sized chromatin was incubated with antibodies for 3 h at 4°C and recovered with protein A agarose beads. ChIP DNA (10 ng for H3K27ac) was processed using NEBNext ChIP-seq library (New England Biolabs) with manufacturer's instructions. The ends of ChIP DNA were repaired by NEBNext Ultra II End Prep enzyme mix and ligated with NEBNext adaptors. The concentration of adaptors was 1.5 µM for H3K27ac ChIP DNA. Adaptor-ligated DNA was selected at 200 bp size using NEBNext sample purification bead and amplified using the adaptor primers (7 cycles for H3K27ac). The fragments are purified using NEBNext sample purification beads. Final libraries were quantified with Qubit dsDNA HS assay (Invitrogen) and were sequenced single-end with a read length of 100 bp on an Illumina NovaSeq system.
identifiers.namespace: GEO
identifiers.id: GSM4413102