alias: GSM3831227
title: GSM3831227: MERS-CoV-infected-untreated-sacrifice-3dpi [1C4]; Mus musculus; RNA-Seq
library_construction_protocol: Total lung RNA extracted from lungs at different times p.i. using Trizol (Invitrogen) was treated with RQ1 RNase-free DNase (Promega) for 10-15 minutes at 37°C to remove genomic DNA contamination. Total RNA samples were converted to Illumina sequencing libraries using Illumina's TruSeq Stranded mRNA Sample Preparation Kit (Cat. # RS-122-2103. Please see www.illumina.com for a detailed list of kit contents and methods). In summary, 500 nanograms of total RNA were oligo-dT purified using oligo-dT coated magnetic beads, fragmented and then reverse transcribed into cDNA. The cDNA was adenylated, ligated to dual-indexed (barcoded) adaptors and amplified using 15 cycles of PCR. Final library size distribution is validated using capillary electrophoresis and quantified using fluorimetry (PicoGreen). Indexed libraries were then normalized, pooled and then size selected to 320bp +/- 5% using the Pippin HT instrument.
identifiers.namespace: GEO
identifiers.id: GSM3831227