alias: GSM1920264
title: GSM1920264: Obese_MNase_rep5; Homo sapiens; MNase-Seq
library_construction_protocol: Spermatozoa were lysed in 0.075% lysolecithan (Sigma) for 15 minutes on ice. After 2 rounds of Dithiothreitol treatment, chromatin was fragmented by Micrococcal Nuclease (100 units for 1 hr at 37˚C) (New England Biolabs) to obtain fragments of approximately 147 bp. The histone-bound DNA fragments were separated from the protamine-bound DNA by centrifugation. Histones were removed by Proteinase K (Qiagen) treatment and Phenol:Chloroform extraction. DNA was ligated to TruSeq (Illumina) sequencing adapters using the standard Illumina protocol, and sequenced on a HiSeq Illumina platform.
identifiers.namespace: GEO
identifiers.id: GSM1920264