alias: E-MTAB-6917:Zeb1 0hr ChIP_p
title: Illumina HiSeq 2500 paired end sequencing; ZEB1 ChIP-seq Data from Control CD8+cDC1
design: ZEB1 ChIP-seq Data from Control CD8+cDC1
library_construction_protocol: The CD8α+ DC lines were grown in IMDM medium supplemented with 10% FBS and antibiotic solution (Gibco), 5x10-5M 2-mercaptoethanol,sodiumbicarbonate, hepes. The cells were maintained at 37°C in a humidified incubator with 5% CO2. DNA-Chromatin was fixed and cross-linked using 16% formaldehyde and quenched using 2.5M glycine. Thereafter, the fixed and cross-linked samples were fragmented by sonication in a Bioruptor. the fragments between 200 to 500 bp was further processed and immuno-precipitated with Zeb1 Antibody and checked with qPCR. Rest of the samples were preserved for preparing library and sequencing. For ChIP-seq library preparation, 30 µl ChIP-DNA was processed for library preparation according to ChIP-seq library preparation recommended protocol (NEB)
identifiers.namespace: Institute of Life Sciences, Nalco Square Chandrashekharpur, Bhubaneswar Odisha-751023
identifiers.id: E-MTAB-6917:Zeb1 0hr ChIP_p