alias: GSM2049374
title: GSM2049374: RI24; RNA-Seq; Oryza sativa Indica Group; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol (Invitrogen, USA) from each sample according to the manufacturer's protocol. NanoDrop ND-1000 spectrophotometer (NanoDrop, USA) was used to quantify the concentration of total RNA. RNA-seq was performed via HiSeq 2000(Illumina, USA) at Beijing Genome Institute (BGI) (Shenzhen, CHN).Briefly, oligo(dT) magnetic beads was employed to enrich mRNA and then interrupted mRNA to short fragments (about 200 bp), the mRNA fragments was treated as templates to synthesizethe first and second strand cDNA. Following by ligating sequencing adaptors to the fragments, the fragments were purified by agarose gel electrophoresis and enriched by PCR amplification. HiSeq 2000 was used to sequence the library products.
identifiers.namespace: GEO
identifiers.id: GSM2049374