alias: GSM3532919
title: GSM3532919: chicken_high_lung3; Gallus gallus; miRNA-Seq
library_construction_protocol: Three adult females for each population of the five species from distinct altitudes (600 m, 2000 m, and 3000 m) were used (Table 1). Animals were humanely killed to ameliorate suffering. Upon excised from the corpse, a piece of tissue fragments from six organs including heart, liver, spleen, lung, kidney and skeletal muscle(longissimus muscle for pig, cattle, yak and sheep, and pectoral muscle for chicken) were placed immediately frozen in liquid nitrogen, and then stored at −80 °C until use. Of note, the skeletal muscle for pig, cattle, yak sheep were longissimus muscle, and the pectoral muscle for chicken. Total RNA were extracted using a standard TRIzol (Takara, Japan) protocol. Small RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM3532919