alias: GSM2465810
title: GSM2465810: BSseq_RIL258_rep2; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Harvested leaf tissue was pulverized using the Qiagen TissueLyser II. For bisulfite-sequencing, DNA was extracted using the Qiagen DNeasy Plant Mini kit (cat. No. 69104). For RNA-seq, total RNA was isolated using the RNeasy plant mini kit (Qiagen, cat. no. 74903). For bisulfite sequencing, between 100 and 500ng DNA was bisulfite-converted using the MethylCode bisulfite conversion kit (Invitrogen). Libraries were prepared using the EpiGnome Methyl-seq kit from Epicentre (now the TruSeq DNA methylation kit from Illumina, cat no. EGMK81312), using all bisulfite-converted DNA. Reads were sequenced on an Illumina HiSeq 2000 to obtain 40x40, 50x50 and 100x100bp paired-end reads. Illumina TruSeq DNA methylation protocol with index CAGATC sequenced on 12/09/16.
identifiers.namespace: GEO
identifiers.id: GSM2465810