alias: E-MTAB-2568:SRO9-Total-4
title: AB SOLiD 4 System sequencing; Analysis of the role of yeast Sro9p in controlling the translation of specific mRNAs
design: Analysis of the role of yeast Sro9p in controlling the translation of specific mRNAs
library_construction_protocol: Grown in SCD media at 30C to exponential phase (OD600 0.5 - 0.7), pelleted by centrifugation and snap frozen into liquid nitrogen Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit. Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1_l glycogen, washed twice with 70% ethanol and re-suspended in 10_l DEPC water.  Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. cDNA was then amplified and barcoded with SOLiD RNA barcoding Kit and samples purified using PureLink PCR Micro Kit
identifiers.namespace: THE UNIVERSITY OF MANCHESTER
identifiers.id: E-MTAB-2568:SRO9-Total-4
identifiers.namespace: The University of Manchester
identifiers.id: E-MTAB-2568:SRO9-Total-4