alias: GSM2048870
title: GSM2048870: 02-02-Normal; Homo sapiens; RNA-Seq
library_construction_protocol: OCT-embedded colon biopisies were sectioned at 9-um thickness onto PEN membrance slides and subjected to LCM of stromal and epithelial cells onto separate caps. RNA was extracted from caps using Picopure RNA isolation kit following manufacturer's protocols. Half of extracted RNA was used for each library preparation panel RNA libraries were prepared for sequencing using standard IonTorrent RNA Ampliseq protocols for two separate panels (Apoptosis and custom Senesence). Libraries were barcoded with IonXpress Adapters. Targetted RNA-seq, Barcoded (provided in the barcode.txt) to run 4 samples per 318 chip each raw data file is named as 'Patient#-Biopsy#-Epithelia/Stroma-Apoptosis/Senescence Panel-ACF/Normal' and the original file names  (associated with each sample)/their checksums are included in the barcode.txt.
identifiers.namespace: GEO
identifiers.id: GSM2048870