alias: E-MTAB-4262_2:R3_15mM_24h_p
title: Illumina HiSeq 2000 paired end sequencing; Expression analysis of Arabidopsis seedlings 3h and 2 h after transfer to sucrose
design: Expression analysis of Arabidopsis seedlings 3h and 2 h after transfer to sucrose
library_construction_protocol: 3 hours and 24 hours after transfer, seedlings were harvested in liquid nitrogen, put in RNAlater ice solution and incubated at -20°C for at least one week, after which the third leaf was micro-dissected under a binocular light microscope.  Seedlings are grown on a mesh covering MS media without carbon source, and subsequently transferred at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose.  Transfer at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose Leaves were frozen in liquid nitrogen and RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant Mini Kit (Qiagen). Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). Briefly, polyA-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina).
identifiers.namespace: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
identifiers.id: E-MTAB-4262_2:R3_15mM_24h_p