alias: GSM3532929
title: GSM3532929: Bulk lung rep1; Mus musculus; RNA-Seq
library_construction_protocol: The excised tissues were immediately washed in PBS. After washing, each tissue was ground into powder with liquid N2. RNA extraction was performed using Trizol. We used mRNA Capture Beads (VAHTS mRNA-seq v2 Library Prep Kit for Illumina, Vazyme) to extract mRNA from total RNA. A PrimeScript Double Strand cDNA Synthesis Kit (TaKaRa) was used to synthesize double-stranded cDNA from purified polyadenylated mRNA templates according to the manufacturer's protocol.  We used TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) to prepare cDNA libraries for Illumina sequencing
identifiers.namespace: GEO
identifiers.id: GSM3532929