alias: GSM2809857
title: GSM2809857: ChIP-HNF1b, reChIPseq-IgG (sample set 3); Mus musculus; ChIP-Seq
library_construction_protocol: Kidneys were dissected from 2-3-month-old WT mice and chopped into fine pieces.  After digestion with 0.5 mg/ml collagenase I (Worthington) for 15 min at 37 °C, the kidney/collagenase mixture was pushed through a 70 µm cell strainer.  ACK lysis buffer was then used to remove red blood cells. Yields were ~8 million cells per mouse.  Cells were cross-linked with disuccinimidyl glutarate for 30 minutes followed by 1% formaldehyde for 10 minutes, then glycine quenched.  The samples were sonicated and subjected to immunoprecipitation.  Precipitates were washed and DNA was purified. KAPA Hyper Prep Kit (Kapa Biosciences)
identifiers.namespace: GEO
identifiers.id: GSM2809857