alias: GSM2120823
title: GSM2120823: testis_H3K27ac_ChIP-seq_R2; Danio rerio; ChIP-Seq
library_construction_protocol: Whole brains, hearts and testis were dissected from adult zebrafish and separated in biological replicates. For the chromatin immunoprecipitation assays, around 2.5x10^8 cells were used for each replicate. Cells were crosslinked with 1% formaldehyde for 15 minutes and subsequently formaldehyde was quenched with glycine (0.125 M). Chromatin was sonicated and immunoprecipitated with 100 μl of antibody/magnetic bead mix. To prepare this mix, 6 μg of H3K27ac antibody were incubated with magnetic beads overnight at 4 degrees C. For each ChIP assay, 50 μl of sonicated chromatin was kept as input DNA. The immunoprecipitated chromatin and inputs were reverse-crosslinked. All samples were treated with RNase A and proteinase K and resuspended in 30 μl of 10 mM Tris-HCl pH 8. DNA fragments with an average size of 1750 bp were used for single-end library preparation following standard Illumina protocols.
identifiers.namespace: GEO
identifiers.id: GSM2120823