alias: ADA-ARCH-08-post
title: Archaeal feces sequencing of prediabetic subjects with obesity after antibiotic therapy - methane non-eradicated subject
design: Archaeal 16S rRNA gene amplicons were generated using high-fidelity Platinum SuperFi Polymerase and the primers ARC344F (5 - TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACGGGGYGCAGCAGGCGCGA  3) and Arch806R (5 - GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACVSGGGTATCTAAT  3). Amplicon quality was assessed using the DNA 1000 assay on an Agilent 2100 Bioanalyzer. Amplicons were then uniquely indexed in a PCR reaction using Nextera XT Index Kit v2 Sets A-D and KAPA HiFi HotStart Ready Mix. Library enrichment was performed using 8 cycles of PCR.
identifiers.namespace: SUB4205496
identifiers.id: ADA-ARCH-08-post