alias: CON1
title: Different feeding strategies  on Indian cattle and its effect on rumen methanon diversity
design: Amplicon libraries  were prepared using the Nextera XT Index Kit (Illumna Inc, USA). Archaea  specific primers (forward: ACGGGGYGCAGCAGGCGCGA; reverse:  GGACTACVSGGTATCTAAT) were used for the amplification of 16s rDNA. Amplicon  libraries were purified by AMPureXP beads and quantified using Qubit  fluorometer. The amplified libraries were analyzed on 4200 Tape Station  system (Agilent Technologies) using D1000 Screen Tape.
identifiers.namespace: SUB7738547
identifiers.id: CON1