alias: GSM2735895
title: GSM2735895: RPF MG1655 csrA::kan 2; Escherichia coli str. K-12 substr. MG1655; OTHER
library_construction_protocol: Cell pellets were thawed, lysed with bead beating, and treated with micrococcal nuclease to release single monosomes. Ribosomes were isolated by ultracentrifuging lysate over a sucrose cushion. Ribosome protected fragments  (RPF) were extracted from the pelleted ribosomes with miRNeasy RNA Extraction Kit (Qiagen) and size selected between 20 and 35 bases with denaturing PAGE. RNA samples were ligated to a 3' adaptor and reverse transcribed. The cDNA was circularized to add a 5' adaptor then PCR amplified to add sample specific barcodes.
identifiers.namespace: GEO
identifiers.id: GSM2735895