alias: GSM2888389
title: GSM2888389: YC592-9d-litter5-HET; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from a single testis per animal, after removing the tunica albuginea, was extracted using the RNeasy Plus Mini Kit containing a genomic DNA eliminator column (Qiagen), according to manufacturers' instructions. Sequencing was performed at the Integrated Genomics Operation of MSKCC. 500 μg of total RNA underwent polyA capture and library preparation using the Truseq Stranded Total RNA library preparation chemistry (Illumina). Sequencing was performed using the Illumina HiSeq platform (2×50-bp paired-end reads) to generate on average, 44 million paired reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM2888389