alias: GSM2808301
title: GSM2808301: Male_WT_mESC_HiC_1; Mus musculus; Hi-C
library_construction_protocol: Hi-C libraries were generated with the in-situ ligation protocol using the HindIII restriction enzyme.  Briefly, ~25 million cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature.  Then, the chromatin was digested with HindIII, end-labelled with biotin-14-dCTP, and in-situ ligation was performed.  Following phenol-chloroform extraction, the biotin was removed from unligated ends, and the DNA was sheared by using a Covaris S220 instrument. After A-tailing, biotin pull-down and adapter ligation, paired-end sequencing was performed on a HiSeq instrument.
identifiers.namespace: GEO
identifiers.id: GSM2808301