alias: Murcia-eidolon_metagenomic-lung
title: Metagenomic sequencing of African fruit bat throat sample
design: Size-selected amplified library preparation of metagenomic lung sample
library_construction_protocol: For each sample type 1ug of DNA was prepared for sequencing. DNA library was sheared to a size of 200-300bp and purified, blunt-end repaired and ligated to Illumina sequencing primers. Ligation products were purified and size selected by agarose gel electrophoresis. Following gel purification, library was PCR amplified in triplicate for each sample for 10 cycles using Phusion DNA polymerase and primers to the Illumina adapters. After this step, primers were removed and the library was quantified by qPCR and diluted to 40nM for cluster generation.
identifiers.namespace: TGAC
identifiers.id: Murcia-eidolon_metagenomic-lung