alias: GSM2465819
title: GSM2465819: RNAseq_col_rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Harvested leaf tissue was pulverized using the Qiagen TissueLyser II. For bisulfite-sequencing, DNA was extracted using the Qiagen DNeasy Plant Mini kit (cat. No. 69104). For RNA-seq, total RNA was isolated using the RNeasy plant mini kit (Qiagen, cat. no. 74903). For RNA-seq, libraries were constructed from 1ug RNA using the RNA TruSeq stranded library kit (Illumina, uses polyA selection) using 15 cycles of amplification. Reads were sequenced on an Illumina HiSeq2000 using a 40bp single-end protocol. Illumina TruSeq RNA stranded protocol sequenced on 07/24/2015 with barcode TGACCA.
identifiers.namespace: GEO
identifiers.id: GSM2465819