alias: GSM3304328
title: GSM3304328: ADR_5; Homo sapiens; RNA-Seq
library_construction_protocol: For each biological replicate, 15 organoids were pooled and pelleted at 1000g for 1 minute. The supernatant was removed and the organoids were washed twice in D-PBS without calcium and magnesium, before homogenization in lysis buffer and RNA extraction using the PureLink RNA Mini Kit (Thermo Fisher). Purified RNA was treated with DNase I (Thermo Fisher) Purified RNA was sent on dry ice to Macrogen for rRNA-depletion using TruSeq Stranded RNA with Ribo-Zero (Human)
identifiers.namespace: GEO
identifiers.id: GSM3304328