alias: GSM2047022
title: GSM2047022: capture in-situ Hi-C; Homo sapiens; OTHER
library_construction_protocol: In situ Hi-C was performed as per Rao et al (2014) using MboI to digest chromatin. Thirty six PCR reactions of 9 cycles each using 25 ng of DNA template  were performed and an Illumina sequencing library was prepared on the material. For the capture step, the Hi-C library was hybridyzed to 81,735 biotinylated 120 bp custom RNA probes purchased from Custom Arrays designed on 4 nearest MboI restriction ends near human RefSeq promoters. Ten cycles of PCR were performed on the captured Hi-C library for sequencing.
identifiers.namespace: GEO
identifiers.id: GSM2047022