alias: GSM3227808
title: GSM3227808: Foxc1_ChIPSeq; Mus musculus; ChIP-Seq
library_construction_protocol: Adult mouse was perfused with magnetic beads. The kidney cortex was dissected and digested. Glomeruli with magnetic beads were collcted by MPC. Isolated glomeruli were fixed and sonicated. ChIP were carried out with specific antibody. Libraries were prepared according to Illumina's instructions. Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation, libararies were generated with KAPA library amplificateion kit. Libraries were sequenced on Illumina Hiseq 2500.
identifiers.namespace: GEO
identifiers.id: GSM3227808