alias: Sim_38H_RNA
title: Simul-seq of gDNA/RNA:Fibroblast rep2 RNA
design: Nucleic acids were extracted using the RNeasy Mini kit (Qiagen:74104) per manufacturer's instructions, except the optional DNase I treatment was not performed. Ribosomal RNA sequences were depleted from the total nucleic acid mixture using Ribo-Zero gold (Illumina:MRZG126), with the standard 70 °C hybridization step changed to 65 °C. Simul-seq library preparation was performed as described in Reuter, JR, Spacek, DV, Pai, RK and Snyder MP (2016). Simul-seq: combined DNA and RNA sequencing for whole-genome and transcriptome profiling. Nat Methods. doi: 10.1038/nmeth.4028. Subsequent libraries were then sequenced using the Illumina HiSeq 2000 platform.
identifiers.namespace: SUB1637552
identifiers.id: Sim_38H_RNA