alias: GSM1920474
title: GSM1920474: SF100 I; Salmonella enterica subsp. enterica serovar Typhimurium; RNA-Seq
library_construction_protocol: Using Rneasy kit (Qiagen) plus Qiashredder columns. The planktonic cultures were treated according to the ScriptSeq v2 protocol. The tumor samples were processed using a customized protocol (Dötsch et al., PloS ONE, 2012). Briefly, eukariotic RNA was depleted using MICROBEnrich Kit (both for tumor samples and control sampels), subsequently bacterial rRNA was depleted using MICROBExpress kit with the Pseudomonas module (Ambion) and fragmented by sonication using Covaris Adaptive Acoustics device (Covaris). Upon ligation of barcoded adapters, reverse transcription with SuperScript II kit (Invitrogen) followed. Amplification was done by PCR using Illumina platform compatible primers. Established cDNA library was normalized by double strand specific nuclease treatment (Evrogen) according DSN Normalization Sample Preparation Guide from Illumina.
identifiers.namespace: GEO
identifiers.id: GSM1920474