alias: GSM3376122
title: GSM3376122: WGBS_mm_dLGN_CD1_P6; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from mouse dLGN tissues using DNeasy mini Kit (Qiagen) according to the manufacturer's instructions. One microgram genomic DNA per sample was used for library construction following the standard Whole Genome Bisulfite Sequencing (WGBS) method. One microgram mouse genomic DNA was spiked with 0.02% unmethylated cl857 Sam7 Lambda DNA (Promega) and sonicated to 200-bp fragments with Covaris M2 (AB). After end repair, dA tailing, the DNA fragments were ligated with cytosine-methylated Illumina TruSeq DNA adapters using T4 DNA ligase (NEB) overnight. After purification, Adapter-ligated DNA fragments were subject to bisulfite conversion using the EpiTect Bisulfite Kit (Qiagen). After bisulfite conversion, the single-stranded uracil-containing DNA was subjected to 12 cycles of PCR reaction with Illumina TruSeq PCR primers and 2.5 U Pfu TurboCx Hotstart DNA polymerase (Agilent) to recover enough DNA for sequencing. The recovered libraries were sequenced on Hiseq 4000 platform (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM3376122