alias: GSM4663525
title: GSM4663525: HEX_2; Mus musculus; ATAC-seq
library_construction_protocol: Frontal cortex was bilaterally dissected from the brain and frozen in either liquid nitrogen, hexane, or slow-freezing medium. Total nuclei were then extracted using sucrose gradient centrifugation. Neuronal (NeuN+) nuclei were isolated from non-neuronal nuclei (NeuN-) with fluorescence-activated nuclei sorting  using an anti-NeuN antibody conjugated to AlexaFluor-488 on a FACSAria instrument. For a detailed protocol for nuclei isolation and purification of neuronal nuclei, see Kundakovic et al, 2017 (PMID:27113501). ATAC-seq libraries were prepared according to Buenrostro et al. 2013 (PMID:24097267) with some modifications. Illumina Nextera DNA Library Prep kit (FC-121-1030) and Nextera Index kit (FC 121-1011) were used to prepare the libraries.
identifiers.namespace: GEO
identifiers.id: GSM4663525