alias: GSM4271905
title: GSM4271905: WT_d170-2; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were dissociated to single cells with papain (Worthington) to 1 mg ml-1 and 5 μL DNase (Roche) per mL, using 200 μL papain mix per organoid.  After 1-2 hours when organoids appeared fully dissociated the reaction was quenched with media containing 10% FBS (Gibco).  Single cells were resuspended in HBSS (Gibco) and 0.1 mg mL-1 BSA at 120,000 cells per mL Illumina nextera XT multiplexed with N70X indices according to the Dropseq protocol
identifiers.namespace: GEO
identifiers.id: GSM4271905