alias: GSM2121269
title: GSM2121269: M3H_M3; Vernicia montana; RNA-Seq
library_construction_protocol: Total RNA was extracted from the plant roots and root-stem transition region using the EASYspin plant RNA mini kit (Aidlab, Beijing, China) QIAGEN RNeasy plant mini kit (QIAGEN, Valencia, CA). RNA integrity was confirmed using the UV-Vis Spectrophotometer Q5000 (Quawell, USA). All RNA samples displayed a 260/280 ratio greater than 2.0 and RNA integrity numbers (RIN) ≥8.0. mRNA was purified from 30 µg of total RNA using Sera-mag Magnetic Oligo(dT) Beads (Illumina) and fragmented into small pieces using divalent cations under elevated temperature. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2121269