alias: GSM3831424
title: GSM3831424: H3K27ac ChIPseq 3M db/db; Mus musculus; ChIP-Seq
library_construction_protocol: We purified the pancreatic islets (Kuo et al, JBC 2016), applied leptomycin B treatment, and performed FoxO1-Venus ChIPseq using antibody against GFP, purchased from abcam (ab290). For H3K27ac ChIP, we FAC-sorted the beta cells, genetically labeled with RIP-Cre and ROSA26-tomato alleles, from db/db mice and littermate controls. H3K27ac antibody was purchased from Active Motif (39133). ChIP DNA libraries were prepared from ChIP DNA with the following steps: end-repair, dA-addition, adaptor ligation and PCR amplification. The resulting ChIPseq DNA libraries were quantified with Bioanalyzer (Agilent), and sequenced on Illumina NextSeq 500 with 75-nt reads and single-end.
identifiers.namespace: GEO
identifiers.id: GSM3831424