alias: GSM1870021
title: GSM1870021: -Pit Shoot 7 dat Meth; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: For Bisulfite-Seq Arabidopsis thaliana genomic DNA from two biological replicates was extracted using the DNeasy system (Qiagen, Valencia, CA, USA). For Bisulfite sequencing libraries,DNA was fragmented by sonication with a Sonicator to a mean size of approximately 250 bp, the preparation of MethylC-Seq libraries was carried out using Pico Methyl-Seq Library Prep Kit (Zymo Research) according to manufacturer instructions. Ultra-high-throughput pair-end sequencing was carried out using the Illumina HiSeq 2000 according to manufacturer instructions.
identifiers.namespace: GEO
identifiers.id: GSM1870021