alias: GSM2888588
title: GSM2888588: WT-1-IP; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: NET-Seq analysis was performed from two biological replicates of Rpb1-3FLAG (WT) and ino80-delta Rpb1-3FLAG (ino80) cells. Libraries were constructed according previously published protocol (Churchman and Weissman, 2012), with minor modifications, starting from 1L of exponentially growing cells. Ligation of DNA 3'-linker was performed as described (Marquardt et al., 2014), starting with 2-3 µg of purified nascent RNA. Ligated nascent RNA was then submitted to alkaline fragmentation for 20 minutes at 95°C. Single-end sequencing (50 nt) of the libraries was performed on a HiSeq 2500 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM2888588