alias: GSM3680213
title: GSM3680213: STZ GLP-1/estrogen + insulin; Mus musculus; RNA-Seq
library_construction_protocol: Islet isolation was performed by collagenase P (Roche) digestion of the adult pancreas. For single cell suspension of islets, the islets were handpicked in an 1.5 mL Eppendorf tube, pelleted (800 rpm, 1 min) washed with PBS (-Mg/Ca, Gibco) and digested with 0.25% Trypsin with EDTA (Gibco) at 37°C for 8 min. Mechanical disaggregation every 2-3 min was required. Single cell libraries were generated using the ChromiumTM Single cell 3`library and gel bead kit v2 (PN #120237) from 10x Genomics. Libraries were sequenced on the HiSeq4000 (Illumina) with 150bp paired-end sequencing of read2.
identifiers.namespace: GEO
identifiers.id: GSM3680213