alias: GSM2464650
title: GSM2464650: Control CD1a- replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: The Trizol method (Mirneasy RNA extraction mini kit, Qiagen, Valencia, CA) was used to extract total RNA from  CD45+GFP+CD7+CD1a- and CD45+GFP+CD7+CD1a+ T-cell precursors isolated by FACS on day 14 of cord blood cells-OP9DLL1 co-cultures . The Ovation Human FFPE RNA‑Seq System (NuGen) was used to convert 25 ng of total RNA from T-cell precursors into amplified cDNA libraries. The cDNA was sheared using a S220 focused ultrasonicator (Covaris, Woburn, MA) to generate an average fragment size of 300 base pairs. twenty cycles of PCR were used to ampify the libraries. Libraries were sequenced on Illumina Nextseq500 (paired end 75 base pair sequencing)
identifiers.namespace: GEO
identifiers.id: GSM2464650