alias: GSM2809418
title: GSM2809418: 171_72; Mus musculus; RNA-Seq
library_construction_protocol: To obtain RNA, mice were sacrificed 3 days after stroke. Strokes are visible by eye at this time-point, and cortical tissue was dissected at 4°C that contained the stroke core and 1-2 mm of surrounding penumbral tissue, and then snap-frozen on liquid nitrogen and stored at -80°C. RNA was extracted from frozen tissue with Trizol. RNA library preparation was performed using an Agilent Bravo automated liquid handling platform using Illumina TruSeq Stranded Total RNA HT reagents. Final libraries were assessed for quantity and quality on a Fragment Analyzer instrument (Advanced Analytical). Multiplexed paired-end libraries were sequenced on an Illumina HiSeq 4000 instrument at 300 cycles, generating on average 20-30 million reads per library sample.
identifiers.namespace: GEO
identifiers.id: GSM2809418