alias: GSM3529699
title: GSM3529699: 3_0295_009KGenen_s3_tnbc_treated_H3K27Ac; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine.  Chromatin was isolated by the addition of lysis buffer, followed by disruption with a Dounce homogenizer. Lysates were sonicated and the DNA sheared to an average length of 300-500 bp. ChIP reactions were performed in the presence of a constant amount of Drosophila chromatin, corresponding to a minor fraction of the total ChIP DNA, and the Drosophila-specific H2A.v antibody as a means of spike-in normalization amongst different samples. Illumina sequencing libraries were prepared from the ChIP and input DNAs following standard enzymatic steps consisting of end-polishing, dA-addition, and adaptor ligation. The resulting DNA libraries were quantified and sequenced as 150 bp paired-end reads using Illumina's HiSeq 2500. The average size of the sequenced fragments were around 380 bp.
identifiers.namespace: GEO
identifiers.id: GSM3529699