alias: GSM2734428
title: GSM2734428: HUES1 repeat 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were mixed with TRIzol (Invitrogen) and dissolved for 5 min, then spined at 12000g for 5 min at 4℃. Chloroform was added to the supernate and mixed equably, then spined at 12000g for 10 min at 4℃. Chloroform/isopropanol (24:1) were added to the supernate and spined at 12000g for 10 min at 4℃ again. The same volume of isopropanol was added to the supernate and stored at -20℃ for 1 hr, then spined at 13600g for 20 min at 4℃. Sediment was washed by 75% alcohol and spined at 13600g for 20 min at 4℃ twice and RNA was dissolved by Nucease-free water. The purity, integrity and density of RNA was detected by Nanodrop, Agarose gel electrophoresis and Agilent 2100 BioanaLyzer respectively. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2734428