alias: GSM3828660
title: GSM3828660: MED1 IP Clone a, untreated for Mediator ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: Cells we fixed in 1% formaldehyde at RT for 10 minutes and subsequently 2mM disuccinimidyl glutarate , nuclei extracted and chromatin sonicated using a bioruptor (10 cycles, 30s on 30s off). Cleared chromatin was incubated with either anti-MED1  antibody (Bethyl Laboratories, A300-793A), or anti-FLAG (to IP MED24),  (Sigma, F3165) . Input DNA serves as negative control for antibody enrichment. Libraries were prepared using the NEB Next Ultra 2 kit.
identifiers.namespace: GEO
identifiers.id: GSM3828660