alias: GSM3228027
title: GSM3228027: PAD-035; Homo sapiens; RNA-Seq
library_construction_protocol: CD138+ myeloma cells were isolated using magnetic activated cell sorting CD138 MicroBeads (Miltenyi Biotech), resulting in cell suspensions with purity of greater than 90%. Total RNA isolation was carried out using PureLink RNA Micro Kit RNAqueous kit (Invitrogen). Samples were only processed if they had an RNA integrity number of greater than 6, as determined by the RNA 6000 Nano Kit (Agilent). Libraries were prepared from 250ng total RNA with the TruSeq Stranded mRNA LT sample preparation kit (Illumina) according to the manufacturer's instructions, apart from a 10-minute fragmentation and 14 cycles of PCR. The average insert size was 150bp.
identifiers.namespace: GEO
identifiers.id: GSM3228027