alias: GSM4272025
title: GSM4272025: CLL [G70201_GCLL-0103-T-01]; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated and quantified using previously described methods (Boyle et al., 2012). RRBS library generation was performed as previously described (Boyle et al., 2012). Genomic DNA was enzymatically sheered using MspI endonuclease (New England Biolabs, catalogue number R0106L), which exposes a CpG site at the 3'-end. Barcoded Illumina sequencing adapters were added to each end of the DNA strand. The DNA was then subjected to two rounds of sodium bisulfite conversions using the Epitect Bisulfite Conversion Kit (Qiagen, catalogue number 59104). A final, large-scale PCR was conducted on the converted DNA (which uracil is replaced by thymine) and the product was submitted for sequencing.
identifiers.namespace: GEO
identifiers.id: GSM4272025