alias: E-MTAB-3733:sOzzy_B_132t.141127.HiSeq2000.FCB.lane3.R1.fastq.gz
title: Illumina HiSeq 2000 sequencing; Centrosome amplification triggers tumorigenesis in p53 deficient epidermis
design: Centrosome amplification triggers tumorigenesis in p53 deficient epidermis
library_construction_protocol: mCherry-PLK4 transgenic mouse was obtained from Renata Basto (Centre de Recherche , Institute Curie, Paris, France) . K14Cre mice were a kind gift from Elaine Fuchs 24 (Howard Hughes Medical Institute, The Rockefeller University, New York, USA). P53fl/fl mice were obtained from the National Cancer Institute at Frederick 32. K14CRE, mice were mated with PLK4 and P53fl/fl mice Sorted cells or bone marrow genomic DNA were extracted using Qiagen DNeasy Blood& Tissue kit following manufacturerâs protocol. hole genome DNA libraries were created using the Illumina TruSeq DNA sample preparation kit V2 according to the manufacturerâs instructions.
identifiers.namespace: VESALIUS RESEARCH CENTER, VIB, LEUVEN, BELGIUM LABORATORY FOR TRANSLATIONAL GENETICS, DEPARTMENT OF ONCOLOGY, KU LEUVEN, LEUVEN, BELGIUM
identifiers.id: E-MTAB-3733:sOzzy_B_132t.141127.HiSeq2000.FCB.lane3.R1.fastq.gz
identifiers.namespace: Vesalius Research Center, VIB, Leuven, Belgium Laboratory for Translational Genetics, Department of Oncology, KU Leuven, Leuven, Belgium
identifiers.id: E-MTAB-3733:sOzzy_B_132t.141127.HiSeq2000.FCB.lane3.R1.fastq.gz