alias: F_l1_2.fq
title: RNA-seq of Pinctada fucata: Adult foot 1-2
design: The mRNA molecules containing poly (A) were purified using Sera-mag Magnetic Oligo (dT) Beads from the RNA samples. A fragmentation buffer was added to break the mRNA into short fragments. Using these short fragments as templates, the first strand of cDNA was synthesized. The second strand of cDNA was synthesized using the buffers containing dNTPs, RNaseH, and DNA polymerase I. The synthesized cDNA was purified using the QiaQuick PCR extraction kit (Qiagen). The purified cDNA was connected with the sequencing adapters. A range of cDNA fragments (200?ĄŔ?25 bp) were excised from 2% TAE-agarose gel (Certified Low-Range Ultra Agarose, Biorad) using QIAquick Gel Extraction Kit (Qiagen). The library was sequenced using the Illumina/Hiseq-2000 RNA-seq.
identifiers.namespace: Tsinghua University
identifiers.id: F_l1_2.fq