alias: GSM3227968
title: GSM3227968: RS1: Repli_seq_rB_0h_NT_rep1; Mus musculus; OTHER
library_construction_protocol: Replication timing profiles were determined using TimEX as described (Bartholdy et al., PMID: 25987481) with minor modifications.  Briefly, The S/G1 TimEX ratio, proportional to the replication time during S phase, was measured in mature splenic B cells from wild-type C57BL/6NCr mice.  The G1 population was obtained from mature resting B cells (100% G0/G1 arrested), which were isolated using anti-CD43 MicroBeads (Miltenyi Biotech) for negative selection. To obtain the S-phase population, the resting B cells were activated with LPS (25 mg/ml; Sigma), IL-4 (5 ng/ml; Sigma) and RP105 (0.5 mg/ml; BD) for 48 hours (~70% S-phase).   5 million cells were collected for both populations, and whole genome sequencing was performed using standard Illumina library prep and sequenced on HiSeq 2000. libraries were prepared and sequenced following standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM3227968