alias: GSM2316514
title: GSM2316514: Antonis_IgG_2; Homo sapiens; RIP-Seq
library_construction_protocol: after immunopreipitation (or for total cell lysate), RNA was extracted using Trizol and purfied using the PureLink kit (Ambion) whole-transcriptome isolation protocol. RNA libraries were prepared using 200-500 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded Total RNA Sample Prep Kit (Illumina, San Diego, CA).  The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA) and Qubit fluorometry (Invitrogen, Carlsbad, CA).Libraries were sequenced at 4 or 8 samples per lane to generate 15-33 million read pairs per sample following Illumina’s standard protocol using the Illumina cBot and cBot Paired end cluster kit version 3. The flow cells were sequenced as 51 X 2 paired end reads on an Illumina HiSeq 2000 using TruSeq SBS sequencing kit version 3 and HCS v2.0.12data collection software. Base-calling is performed using Illumina’s RTA version 1.17.21.3
identifiers.namespace: GEO
identifiers.id: GSM2316514