alias: GSM3529626
title: GSM3529626: S118 F2 RESTRICTED DAY 135; Ovis aries; RNA-Seq
library_construction_protocol: Longissimus dorsi tissue samples were collected from a subset of male fetuses at day 90 (n = 11, CON = 4, OVER = 3, RES = 4), day 135 (n =10, CON = 3, OVER = 3, RES = 4) and lambs at birth (n = 11, CON = 4, OVER = 4, RES = 3) and stored at -80°C until RNA-seq analyses. RNA was isolated as described in Reed et al. (2014). Briefly, tissue was homogenized in 1 mL Qiazol using a Qiagen Tissuelyser (Qiagen, Valencia, CA) and RNA was extracted using a Qiagen Mini Kit according to the manufacturer protocol (Qiagen). A Turbo DNA Free kit (Ambion, Foster City, CA) was used to remove genomic DNA. RNA quality was determined using a Bioanalyzer analysis system (Agilent Technologies, Santa Clara, CA). A total of 50 ng of RNA (RNA integrity number of 8 or above) from each animal was used to prepare sequencing libraries following Illumina's TruSeq stranded mRNA Sample Preparation Guide for library preparation protocol.
identifiers.namespace: GEO
identifiers.id: GSM3529626