alias: GSM3679125
title: GSM3679125: PTU_WT_rep3_RNA; Mus musculus; RNA-Seq
library_construction_protocol: Approximately 5 mg of liver tissue were homogenized using Ultra-Thorax and RNA was purified using TRIzol-RNA lysis reagent (ThermoFisher) in EconoSpin columns (Epoc Life) according to manufacturer's instructions. Total RNA (1000 ng) was prepared for sequencing using ribosome depletion followed by random primed cDNA synthesis or polydT-mediated cDNA synthesis according to the manufacturer´s (Illumina) instructions. Subsequent library preparation was performed using the NEBNext RNA library preparation kit for Illumina.
identifiers.namespace: GEO
identifiers.id: GSM3679125