alias: GSM1920209
title: GSM1920209: Exd1 -/- ; MIWI2 IP rep1; Mus musculus; RNA-Seq
library_construction_protocol: For preparation of small RNA libraries total RNA was isolated from the lysates or RNA was immunoprecipitated from the protein complexes with the indicated antibodies against PIWI protein and the RNAs were resolved by 15% urea-PAGE. Bands corresponding to piRNAs or the indicated size were excised from the gel and extracted with 400 µl of 0.4M NaCl solution at 25°C overnight. Small RNA sequencing libraries  were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions. Libraries were sequenced on Illumina HiSeq platform (EMBL Heidelberg Gene core facility).
identifiers.namespace: GEO
identifiers.id: GSM1920209