alias: GSM2048228
title: GSM2048228: DMSO_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were trypsinized and total RNA was extracted using the RNeasy kit (Qiagen). From1 µg of total RNA PolyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq Stranded mRNA Sample Preparation Part # 15031047 Rev. D" kit. Adapter-ligated library was completed by PCR with Illumina PE primers (8 cycles). The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation and sequenced for 50 bases in a single-read format (Illumina HiSeq 2000).
identifiers.namespace: GEO
identifiers.id: GSM2048228