alias: GSM4416532
title: GSM4416532: B16_TIL3_1; Mus musculus; RNA-Seq
library_construction_protocol: For isolation of CD8+ T cells from spleen, spleens were collected and dissociated to yield a cell suspension prior to lysis of red blood cells. For isolation of CD8+ T cells from the tumors, tumors were digested with Collagenase I for 30 minutes then Percolled at 44%/67% gradient at 2000 rpm for 20 min (acceleration 4, brake 0) Single cell libraries were prepared according to the protocol for 10X Genomics for 3' Gene Expression. Approximately 10,000 sorted CD45.1+ Va2+ CD8+ CD44+ cells were loaded and partitioned into Gel Bead In-Emulsions (GEM). scRNA libraries were sequenced on a HiSeq4000 (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM4416532