alias: GSM3473791
title: GSM3473791: Experiment 2, patient 6; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from purified neutrophils was extracted using the MagMax -96 Total RNA Isolation Kit (Life Technologies) Using the SuperScript VILO cDNA Synthesis Kit (Life Technologies), 10 ng of RNA was reverse transcribed to make cDNA. The Ion AmpliSeq Library Kit Plus (Life Technologies) and the Ion AmpliSeq Transcriptome Human Gene Expression Core Panel was used to amplify target genes per manufacturer's directions. Each amplicon was then barcoded with the Ion Express Barcode Adapter (Life Technologies), and purified through Agencourt AMPure XP Beads (Beckman Coulter) and freshly prepared 70% ethanol. Finally, libraries were analyzed with High Sensitivity NGS Fragment Analysis Kit (AATI). All libraries were peaked around 200 bp Ampliseq transcriptome using Ion Torrent S5
identifiers.namespace: GEO
identifiers.id: GSM3473791