alias: E-GEUV-1:NA12843.6.M_120119_4
title: RNA-sequencing of 465 lymphoblastoid cell lines from the 1000 Genomes
design: RNA-sequencing of 465 lymphoblastoid cell lines from the 1000                 Genomes
library_construction_protocol: Total RNA was extracted from cell pellets using the                     TRIzol Reagent (Ambion). The pellets had been frozen at ECACC without any                     additives like RNAlater or Trizol. In Geneva they were thawed, 1mL of trizol                     was added in each sample, and the samples were transferred to eppendorf tubes.                     The rest of the protocol followed the manufacturer's guidelines. No DNase                     treatment was done to the RNA samples. Sample processing for sequencing was                     done in a random order in each of the seven laboratories. Library preparation                     was done with TruSeq RNA Sample Prep Kit v2 using the high-throughput                     protocol.
identifiers.namespace: Geuvadis
identifiers.id: E-GEUV-1:NA12843.6.M_120119_4