alias: GSM2047268
title: GSM2047268: SEN hADSC; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were isolated from human subcutaneous white adipose tissue collected during liposuction procedures. Total RNA was extracted to construct RNA-seq library. Total RNA was isolated from 3 different genetic lines of adipose derived stem cells in SR and SEN conditions. Samples were grouped together for SR and SEN. Total RNA preparation has been processed with RiboMinus kit to significantly reduce ribosomal RNA in the sample. All samples have been precipitated with Pellet-Paint and dissolved in depc-treated millipure water. SuperScript™ ds cDNA synthesis was performed with random-primers. GS FLX Titanium General Library Preparation Method was employed to incorporate Multiplex Identifier (MID)-containing adaptors for General (e.g.Shotgun) library preparation. Unidirectional Sequencing of Amplicon Libraries Using GS FLX Titanium emPCR Kits (Lib-L) according to Roche Protocol APP No.001-2009 GS FLX Titanium MID-1 ExST Primer1 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGACGAGTGCGTGAGCGGCCGCGAAGATCAGA-3’ GS FLX Titanium MID-3 ExST Primer1 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGAGACGCACTCGAGCGGCCGCGAAGATCAGA-3'
identifiers.namespace: GEO
identifiers.id: GSM2047268