alias: GSM2808508
title: GSM2808508: gonidia 2; Volvox carteri; RNA-Seq
library_construction_protocol: Cell types were separated into gonidial and somatic cell type fractions and fractions were resuspended in a 1:1 ratio of Buffer C (2% SDS in Tris-Buffered Saline) and Trizol and then immediately flash frozen in liquid nitrogen and stored at -80C. Lysates were thawed and sonicated using a Covaris S220 ultrasonicator after which DNA was removed and RNA purified using the RNeasy Mini Kit (Qiagen). Biological replicate samples of gonidial and somatic total RNA were submitted to the Genome Technology Access Center (GTAC) in the Department of Genetics at Washington University School of Medicine for Illumina-based polyA library preparation and sequencing. oligo-dT selected RNA was fragmented to an average size of ~200nt and reverse transcribed to produce double stranded cDNA using random hexamers. cDNA ends were end repaired, 3' dA-tailed, and then ligated to Illumina sequencing adapters. Adaptor-ligated fragments were amplified for 12 cycles using indexed primers and sequenced on an Illumina HiSeq-2500 with 50 bp single-end reads.
identifiers.namespace: GEO
identifiers.id: GSM2808508