alias: GSM2734684
title: GSM2734684: ChIPSeq WT CD4T +IL2 STAT5B 1; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIP-Seq, and cross-linked with 1% formaldehyde (methanol-free, Pierce, Rockford, IL) at room temperature for 10 min. After sonication, fragmented chromatin equivalent to 10 million cells was immunoprecipitated with control rabbit IgG (Santa Cruz Biotechnology, Dallas TX) or indicated antibodies and Magna ChIPTM Protein A+G Magnetic Beads (Millipore, Billerica MA). ChIP-Seq DNA libraries were prepared using KAPA LTP Library Preparation Kit (Kapa Biosystems, Wilmington, MA) and indexed primers (BIOO Scientific, Austin, TX), and the libraries were then sequenced. For RNA-Seq, KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Wilmington, MA), and each library was indexed using barcoded primers (BIOO Scientific, Austin, TX). Barcoded PCR products were purified on 2% E-Gel, 250-400 bp fragments were purified, quantified on Qbit (Invitrogen), mixed, and sequenced on an Illumina Genome Analyzer II or HiSeq2000 platform. PCR products were barcoded (indexed) and sequenced on Illumina Genome Analyzer II or HiSeq 2000 platform.
identifiers.namespace: GEO
identifiers.id: GSM2734684