alias: GSM2733455
title: GSM2733455: 8 hr Rep1; Pteropus alecto; RNA-Seq
library_construction_protocol: RNA was harvested using RNeasy Mini Kit (Qiagen #74106) following the manufacturer's instructions. Integrity was verified using the Agilent 2100 Bioanalyzer. All samples had a RIN score >9.5. One microgram of total DNase-treated RNA was prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit (Illumina  #RS-122). rRNA-depleted total RNA was fragmented and used for strand-specific cDNA synthesis.  cDNA was then A-tailed and ligated with indexed adapters. Samples were then PCR amplified, purified with AmpureXP beads, and validated on the Agilent 2100 Bioanalyzer and Qubit.  Normalized and pooled samples were run on the Illumina Hiseq 2500 using SBS v3 reagents.
identifiers.namespace: GEO
identifiers.id: GSM2733455