alias: R779
title: WGS of Zea mays: tcb1-f KO1
design: A total amount of 1.0g DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext DNA Library Prep Kit following manufacturer's recommendations and indices were added to each sample. The genomic DNA is randomly fragmented to a size of 350bp by shearing, then DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system) and resulted libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR.
identifiers.namespace: SUB5345240
identifiers.id: R779