alias: 6911x7213_#CVL128_D05
design: We harvested leaves from ~3 weeks old plants grown under long day conditions (16 hrs. light and 8 hrs. at 10ĄC). We extracted DNA in 96 plates with the NucleoMag¨ 96 Plant (Macherey-Nagel) kit according to the manufacturerŐs instructions. We prepared libraries using a slightly modified version of the Illumina Genomic DNA Sample preparation protocol. Briefly, 100 to 200 ng of DNA were fragmented by sonication with Bioruptor (Diagenode). End-repair of sheared DNA fragments, A-tailing and adapter ligation were done with Spark DNA Sample Prep Kit (Enzymatics). ĘNEXTflex-96Ş DNA Barcodes (Bioo Scientific) were used to attach indexes to the sample insert during adapter ligation. Size selection, with median insert size around 400 bp, and library purification were performed with Agencourt AMPure XP Beads (Beckman Coulter). Paired-end (PE) DNA libraries were amplified by PCR for 10-12 cycles. After PCR enrichment, libraries were validated with Fragment AnalyzerŞ Automated CE System (Advanced Analytical) and pooled in equimolar concentration for 96X-multiplex. Libraries were sequenced on Illumina HiSeqŞ 2000 Analyzers using manufacturerŐs standard cluster generation and sequencing protocols in 100 bp PE mode.
identifiers.namespace: SUB1642176
identifiers.id: 6911x7213_#CVL128_D05