alias: miRNA
title: Identification of MicroRNAs in Xenopus ectoderm
design: Tissue samples were lysed in Trizol, and RNA was extracted and treated with DNaseI using the Direct-zol RNA mini-prep kit (Zymo). RNA samples were processed for library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit according to manufacturer’s instructions. Concentrations of the prepared libraries were determined using Quant-iTTM Picogreen dsDNA reagent (Thermofisher). Libraries were sequenced at the M. D. Anderson Sequencing and Microarray Facility.
identifiers.namespace: University of Houston
identifiers.id: miRNA