alias: GSM4413980
title: GSM4413980: RNA-seq of mouse photothrombosis model(sham); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA were derived from cerebral cotex on the ipsilateral side of mouse stroke brain at each time point (1d, 3d, 7d, 14d, 28d).  Sham-operated mice at day 7 post-operation were used as control. Strand-specific RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina after poly(A) selection by the NEBNext poly(A) mRNA Isolation Module (New England Biolabs Inc.).  Samples were barcoded using the recommended NEBNext Multiplex Oligos.  Size range and quality of libraries were verified on the Agilent 2100 Bioanalyzer (Agilent Technologies).  RNA-seq libraries were quantified by quantitative polymerase chain reaction using the KAPA library quantification kit (KAPA Biosystems).  Each library was normalized to 2 nM and pooled in equimolar concentrations.  Paired-end 150 sequencing was performed on an Illumina HiSeq4000 (Illumina, San Diego, CA).  Libraries were pooled and sequenced using two lanes of one HiSeq4000 flow cell to an average depth of 33.6 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM4413980