alias: IHHB20K_region1
title: Subgingival Microbiota in Pediatric Crohn's Disease: Validation Cohort
design: Pediatric Crohn's disease patients were sampled when initiating therapy and again after 8 weeks. Healthy control subjects were sampled at the same interval.
library_construction_protocol: Each amplicon library was constructed by amplifying the16S rRNA gene using the BSF8 and and the BSR357 primer pair. Primers contained DNA barcode sequences such as those described by Hamady et al. 2008, and the recommended 454 adapter sequences. Amplification conditions are described in McKenna et al 2007, with exception that the polymerase used was AccuPrime (Invitrogen, Carlsbad, CA, USA).
identifiers.namespace: UPENNBL
identifiers.id: IHHB20K_region1