alias: GSM1919385
title: GSM1919385: P19_tissue_tumor; Homo sapiens; RNA-Seq
library_construction_protocol: Single cell clusters were lysed using SMARTer Ultra Low RNA Kit (Clontech Laboratories). Whole tissue and cell lines RNA was extracted using RNeasy mini kit (Qiagen). RNA from LCM samples was extracted using RNAqueous-Micro Total RNA Isolation Kit (Ambion) cDNA was synthesised from single cell clusters, 10 pg of whole tissue, 10pg laser-dissected endothelial cells or 10pg primary endothelial cell RNA. To this end, we used the SMARTer Ultra Low RNA Kit (Clontech Laboratories) and long-distance PCR (LDPCR), for 25 cycles, 18, 18 cycles and 18 cycles, respectively. For each sample, cDNA was sheared using the Adaptive Focused Acoustics system (Covaris). Libraries were constructed using NEBNext DNA Library Prep Master Mix Kit (New England Biolabs). All libraries were barcoded using unique indexes and pooled for RNA sequencing
identifiers.namespace: GEO
identifiers.id: GSM1919385