alias: GSM4485293
title: GSM4485293: Obese BC rep10; Homo sapiens; RNA-Seq
library_construction_protocol: Whole blood samples from obese and non-obese breast cancer patients were collected in PAXgene™ blood RNA tubes (PreAnalytiX, Switzerland), then the total RNA was isolated using the PAXgeneTM blood RNA kit (Qiagen, UK). Approximately 2 µg of total RNA was fragmented and end‐repaired using the Illumina directional protocol. Complementary DNA (cDNA) sequencing libraries were constructed using Illumina ®TruSeqTM stranded total RNA sample preparation kit (Illumina, USA) according to the manufacturer's instructions. The libraries were sequenced using the Nextseq 500 platform (Illumina, Singapore) in paired-end 150-bp mode, according to the manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM4485293