alias: GSM2464940
title: GSM2464940: Cyanidioschyzon merolae strain 10D H3_3; Cyanidioschyzon merolae strain 10D; ChIP-Seq
library_construction_protocol: The samples were fixed (Formaldehyde, 1% (v/v)) for 10 min, until addition of glycine (to final concentration of 125 mM, 5 min incubation). Superfluous formaldehyde was removed by three washes with ice cold PBS buffer and the remaining cell pellet was resuspended in 4 ml of Extraction Buffer (50 mm Tris-HCl pH 8, 10 mm EDTA, 1% SDS) with Protease Inhibitor Cocktail (Roche). The samples were sonicated for 5 min with 30 sec ON/ 30 sec OFF cycle using Bioruptor Plus device (Diagenode) and cleared by two rounds of centrifugation (13000 rpm, 4°C, 10 min). Subsequent steps were performed as in the Plant ChIP-seq kit protocol (Diagenode) with higher volume of sample taken aside as an input (1:5 of chromatin for IP). According to the MicroPlex Library preparation kit v1 (Diagenode)
identifiers.namespace: GEO
identifiers.id: GSM2464940