alias: GSM3227948
title: GSM3227948: ES5: END_seq_aB_28h_NT_rep2; Mus musculus; OTHER
library_construction_protocol: Single cell suspensions of B-cells (15 million per agarose plug), T-cells (15 million per agarose plug), MEFS (4 million per agarose plug), HCT116 (7.5 million per agarose plug)were washed in PBS, embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit) and processed as previously described (Canela et al., 2016). Libraries were quantified using with the KAPA Library Quantification Kit (Kapa Biosciences) and sequenced in a NextSeq 550 system (Illumina, 75 bp single end reads). libraries were prepared and sequenced following standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM3227948