alias: 8709R
title: Bacteria in rumen and feces of cow on RFDDGS
design: Three grams (wet weight) of homogenized samples of the rumen fluid and feces from each cow were used for the DNA extraction with a bead-beating method followed by phenol-chloroform extraction. The DNA was quantified using a Nanodrop spectrophotometer following staining using a Quant-it Pico Green dsDNA kit (Invitrogen, Paisley, UK). The DNA samples were stored at -80C until further processing. The V3V4 region of the bacteria 16S ribosomal RNA gene was amplified by PCR (95C for 2 min, followed by 25 cycles at 95C for 30 s, 55C for 30 s and 72C of 30 s with a final extension at 72C for 5 min).
identifiers.namespace: SUB7740733
identifiers.id: 8709R