alias: GSM1919581
title: GSM1919581: AGO4 IP in dcl2/3/4 rep3; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: Total RNA were extracted by Trizol reagent (Invitrogen) followed by PEG precipitation to enrich low molecular-weight sRNAs. The resulting sRNAs were resolved on a 15% denaturing PAGE gel, and visualized by SYBR-Gold (Invitrogen) staining. Gel slices within the range of 18-30nt were excised, and the RNAs were eluted and purified for small RNA library contruction. Small RNA libraries were constructed as previously described (Mi et al., Cell, 2008, 133, 116-127).
identifiers.namespace: GEO
identifiers.id: GSM1919581