alias: GSM4663558
title: GSM4663558: AB5075 adeB::T26ΔadeIJK rep P4a; Acinetobacter baumannii; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using hot phenol method. High quality total RNA was further processed by removing 23S and 16S rRNAs using the MicrobExpress bacterial mRNA enrichment kit (Life Technologies, USA). Final concentrations and purity grades of the samples were determined using Bioanalyzer 2100 (Agilent, USA). Samples with the amount at least 0.5-1 µg were used for library construction and RNA sequencing. Libraries of depleted RNA were prepared using the Illumina TruSeq RNA LT v2 kit according to the manufacturer's instructions. RNA libraries were prepared for sequencing using Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM4663558