alias: GSM1671615
title: GSM1671615: Rpf2-HTTP; Saccharomyces cerevisiae BY4741; RNA-Seq
library_construction_protocol: Lysate were clarified and a two-step IP was performed with one in denaturing condition (6M guanidinium), RNA.Rrp5 complexes were seperated by SDS-PAGE, transferred to a nitrocelluose membrane and extracted. Protein was removed by Prrteinase K digestion and RNAs purified by phenol:chloroform extraction.  Extracted RNAs were converted to cDNA and PCR amplified. PCR products between ~150 and 250 bp were gel extracted and submitted forTorrent Sequencing.
identifiers.namespace: GEO
identifiers.id: GSM1671615