title: GSM1924878: gd-Sox13lo_H3K4me3-ChIPseq; Mus musculus; ChIP-Seq
library_construction_protocol: 1 million cells for SOX13 ChIPseq and 0.25 milion cells for H3K4me3 and H3K27me3 were sorted (gd+, CD24+, CFPhi or CFPlo/-), crosslinked with 1% formaldehyde, sonicated, and then subjected to chromatin immunoprecipitation with anti-SOX13, anti-H3K4me3 and anti-H3K27me3 antibodies. 300-500 bp DNA fragments were obtained after reverse-cross linking. ChIP DNA fragments were processed for ChIPseq with Illumina adapters and Illumina primers (Primer1.1 and Primer2.1). After adapter ligation, DNAs were PCR-amplified for 17 cycles and library fragments of 300-500 bp were extracted from an agarose gel.
attributes.tag: GEO Accession
attributes.value: GSM1924878