title: GSM4116424: ATAC-Seq d0; Mus musculus; ATAC-seq
library_construction_protocol: For ChIP-seq, DNA libraries were prepared with the KAPA LTP Library Preparation Kit and barcoded with NEXTflex DNA barcodes. For ATAC-seq, Nuclei were then used for the transposition reaction with the Nextera DNA Library Preparation kit at 37C for 30 min. DNA was purified with the Zymo DNA recovery kit. For RNA-seq, RNA was extracted using the Zymo RNA miniprep kit (Zymo Research), and 500 ng RNA was used for library preparation with the Kapa mRNA HyperPrep Kit (KK8580, Kapa Biosystems) and indexed with NEXTflex DNA Barcodes-24. After the final amplification, samples were loaded onto 2% E-Gel pre-cast gels (ThermoFisher), and 250 to 400 bp DNA fragments were recovered and purified with Zymoclean Gel DNA Recovery Kit (Zymo Research). After quantification by Qubit (Invitrogen), barcoded samples were mixed and sequenced on an Illumina Hi Seq3000 system.
attributes.tag: GEO Accession
attributes.value: GSM4116424