title: GSM4548394: Total_RNA_NIP_Rep1_12hr_CPA; Mus musculus; RNA-Seq
library_construction_protocol: For RNA seq, we extracted the total RNA from day2 motor neurons after 12, 36 hrs of treatment 20uM CPA. The media was then removed and the cells were washed with 10 ml chilled PBS. Following removal of the PBS, 800 ul of lysis Buffer (Qiagen RNeasy Mini Kit) was added and the cells were  scraped off the plate and transferred to a pre-chilled eppendorf tube, recovering ~1 ml of lysate per sample. We processed the lysate according to the Qiagen RNeasy kit extraction protocol. We also performed in-column DNAse digestion using RNAse-Free DNase (Qiagen). The total RNA was then used for RNAseq library following KAPA RNA HyperPrep Kit with RiboErase (HMR) protocol. We used Agilent TapeStation to confirm the  quality and quantity of total RNA and sequence library during different stages of RNAseq library preparation. RNA libraries for RNA seq were prepared according to the KAPA RNA HyperPrep Kit with  RiboErase (HMR) protocol.
attributes.tag: GEO Accession
attributes.value: GSM4548394