title: GSM4161776: L0125_UNT_rep1_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total cellular RNA was extracted using the RNeasy Mini kit (Qiagen, Hilden, Germany) and purified using RNeasy columns according to the manufacturer's instructions. The integrity of RNA was checked using the Agilent's 2100 Bioanalyzer. The same amount of RNA from two independent experiments were used (total amount - 500ng of RNA). In total, 12 strand-specific polyA enriched RNA libraries were prepared using the KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer's protocol (Kapa Biosystems, Wilmington MA, USA). Libraries were run in the rapid run flow cell.
attributes.tag: GEO Accession
attributes.value: GSM4161776