title: GSM4162158: ML-109; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids culture harvested for RNA extraction using TRIzol. RNA quality was confirmed by Nanodrop measurement of OD 260/280 and 260/230 ratios and stored at -800C under ethanol. 1ug RNA was used for RNA-seq library preparation. Directional cDNA libraries made from cellular mRNAs were generated from the samples using the Illumina TruSeq RNA library kit and sequenced (paired-end sequencing of 100 bp reads) in the Genomics Core at the University of Chicago on an Illumina HiSeq2500, according to standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4162158