title: RNA-Seq of rootstock potato in tomato/potato heterografting system, biological repeat2
design: total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer 5X . First strand cDNA was synthesized using random hexamer primerand M-MuLV Reverse Transcriptase RNase H- . Second strand cDNA synthesis wassubsequently performed using DNA Polymerase I and RNase H. Remaining overhangswere converted into blunt ends via exonuclease/polymerase activities. After adenylation of3  ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization.