title: GSM3475501: WT rep1_smRNAseq; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: 300 pairs of testes  were removed and Total RNAs were extracted by the Trizol method. For small RNA sequencing, 2.5 µg of total RNAs were treated with DNase I (Amplification Grade, ThermoFisher) and recovered by RNA Clean & Concentrator-5 columns (Zymo). Libraries were generated using reagents from the TruSeq Small RNA Library Prep Kit (Illumina), while following a previously published protocol from (Wickersheim & Blumenstiel 2013) that uses a terminator oligo to deplete Drosophila 2S rRNA from the final sequencing libraries. 
attributes.tag: GEO Accession
attributes.value: GSM3475501