title: Asian seabass genome sequencing PacBio kidney library
design: Asian seabass kidney genomic DNA was used for generating a ~20 kb insert size library which was sequenced using 105 SMRT cells (1X180 minutes movie) on PacBio RSII using C3-P5 chemistry (DNALink, Korea). All DNA was size selected (>7 kb) using the Blue Pippin system (Sage Sciences) and samples were sheared with g-TUBEs (Covaris).