title: GSM2123965: P3_F10 - BT20_2500_day0; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy mini kit (Qiagen). The 3’-DGE libraries were prepared from total RNA according to the Single Cell RNA Barcoding and Sequencing method originally developed for single cell RNA-seq (SCRB-seq, Soumillon et al., 2014) and adapted to extracted total RNA. Briefly, Poly(A)+ mRNA from extracted total RNA are converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples are then pooled, amplified and prepared for multiplexed sequencing using a modified transposon-based fragmentation approach that enriches for 3’ ends and preserves strand information.
attributes.tag: GEO Accession
attributes.value: GSM2123965