title: GSM2053304: Odd-Tug1-PD2; Mus musculus; OTHER
library_construction_protocol: Gluteraldehyde crosslinked cells were subjected to nuclear extraction protocol and nuclei were subjected to sonication by BioRuptor. 1% Input Chromatin was set aside and Chromatin was incubated with biotynilated Tug1-Even or Tug1-Odd oligos, DNA was reverse-crosslinked and purified by ethanol precipitation. 10 ng of DNA samples were prepared for Illumina sequencing as the following steps: 1) DNA samples were blunt-ended with T4 DNA polymerase and Klenow polymerase; 2) a dA base was added to the 3' end of each strand by Klenow (exo minus) polymerase; 3) Illumina's genomic adapters were ligated to the DNA fragments; 4) PCR amplification was performed to enrich ligated fragments; 5) Enriched product of ~200-400bp was cut out from gel and purified with QIAquick Gel Extraction Kit. The completed libraries were quantified by Agilent 2100 Bioanalyzer. The libraries were denatured with 0.1 M NaOH to generate single-stranded DNA molecules, captured on Illumina flow cell, amplified in situ. The libraries were then sequenced on the Illumina HiSeq 2000 following the TruSeq Rapid SBS Kit protocol.
attributes.tag: GEO Accession
attributes.value: GSM2053304