title: GSM4116585: ICC_24_Tumor2: FACS24T; Homo sapiens; RNA-Seq
library_construction_protocol: Single-cell suspensions were stained for 7AAD (Invitrogen) per the manufacturer's recommendations, washed with cold PBS, and re-suspended for flow cytometric analyses. Fluorescence-activated cell sorting (FACS) -sorted viable cells (7-AAD-negative) were loaded into a well of a microfluidic chip to generate a cDNA library using a commercial 10x Genomics platform. Single-cell transcriptomic amplification and library preparation was performed using single-cell 3′ v2 chemistry (10X Genomics) according to manufacturer's instructions. The libraries were then pooled and sequenced across six lanes on an Illumina Xten or NovaSeq 6000 system.
attributes.tag: GEO Accession
attributes.value: GSM4116585