title: GSM4417298: Endoc_tri_E17 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA from freshly FACS sorted endothelial cell (EC) populations, from dissociated fetal hearts,  was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). Library preparation (New England Biolabs Nest Ultra RNA library prep Kit) and single read Next-generation sequencing (Illumina HiSeq 2500) were performed at the Genomics Unit at Centro Nacional de Investigaciones Cardiovasculares (CNIC). Total RNA (1 ng) were used to generate barcoded RNA-seq libraries using the Ovation® Single Cell RNA-Seq System (NuGEN/Tecan).  The size of the libraries was calculated using the Agilent 2100 Bioanalyzer. Library concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific)
attributes.tag: GEO Accession
attributes.value: GSM4417298