title: GSM3228269: Control mRNA-seq rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Following control or salt treatment, rosette leaves were collected 4 hrs after first light. Leaves were immediately placed on liquid N2 and ground using a mortar and pestle. RNA was extracted using Qiazol (Qiagen) according to manufacturers instructions. 2 rounds of PolyA+ selection was performed using the Dynabeads mRNADirect kit (Thermo). polyA+ mRNA was then prepared into libraries using the strand-specific sequencing library preparation as previously described (Silverman et al, 2014). Strand specific libraries were constructed as previously described (Silverman, et al, 2014)
attributes.tag: GEO Accession
attributes.value: GSM3228269