title: GSM2992364: KO rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified using spin columns of the RNeasy Mini Kit (Qiagen) according to manufacturer's protocol. Library preparation was made with the TruSeq mRNA-seq Stranded v2 Kit sample preparation (Illumina) according to manufacturer's instructions. One µg total RNA was used for poly(A)-selection and Elution-Fragmentation incubation time  was 8 min to obtain 120-210 bp fragments. Each library was barcoded using TruSeq Single Index (Illumina). After library preparation, Agencourt AMPure XP (Beckman Coulter, Inc.) was performed for 200 to 400 bp libraries size-selection (282 nt average final library size). Each library was examined on the Bioanalyzer with High Sensitivity DNA chip (Agilent), quantified on Qubit with Qubit® dsDNA HS Assay Kit (Life Technologies), diluted to 4 nM and then pulled together at equimolar ratio.
attributes.tag: GEO Accession
attributes.value: GSM2992364