title: Food Waste
design: V4 region of 16S rRNA was amplified using 515F/806R. The PCR was performed with HotStarTaq Plus Master Mix Kit (Qiagen, USA)  following protocols by MR DNA (Shallowater, Texas, USA). Samples were multiplexed using unique dual indices and were pooled together (e.g., 100 samples) in equal proportions based on their molecular weight and DNA concentrations. Pooled samples were purified using calibrated Ampure XP beads. Then the pooled and purified PCR product was used to prepare an Illumina DNA library. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq following the manufacturers guidelines.