title: GSM3073681: M1-WT_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were prepared from WT PMs untreated (Cont-WT) or treated with LPS (100 ng/ml) and IFN-γ (10ng/ml) for 12 hr (M1-WT), and DKO PMs treated with LPS and IFN-γ for 12 hr (M1-DKO). Poly(A) mRNA isolation from total RNAs and fragmentation were performed using the Illumina Truseq Stranded mRNA Sample Prep Kit with poly-T oligo-attached magnetic beads, according to the manufacturer's protocol. Libraries were prepared for multiplex sequencing using Illumina Truseq Stranded mRNA Sample Prep Kit, according to the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM3073681