title: GSM3229695: M3125 PB-1; Fusarium verticillioides; RNA-Seq
library_construction_protocol: After the final hour, 1 mL liquid culture from each tube was pelleted by centrifugation at 8000 g for 5 min at 4 °C, resuspended in 1 mL lysis buffer, and transferred to lysing matrix D tubes (MP Biomedicals, LLC, Santa Ana, CA, USA). The tubes were homogenized with a FastPrep-24TM 5G instrument (MP Biomedicals) at 6 m/s with 2 pulses of 30 s and a 1 min intervening pause at room temperature. Total RNA was extracted from the homogenized samples with a PureLink® RNA Mini Kit (Thermo Fisher Scientific Inc., MA, USA) following the manufacturer's protocol. Sequencing libraries were constructed with Illumina Truseq DNA LT sample prep kit (Illumina Inc., San Diego, CA, USA) following the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM3229695