title: Profiling miRNAs in mouse embryonic stem cells with mutations in the Dgcr8 locus
design: Profiling miRNAs in mouse embryonic stem cells with mutations in the Dgcr8 locus
library_construction_protocol: Cells were  lysed with Trizol while sub-confluent. Large RNA was removed from the small RNA fraction using the RNeasy Mini Kit (Qiagen), and small RNAs were collected by isopropanol precipitation and quantitated on an Agilent Technologies 2100.
attributes.tag: Experimental Factor: Genotype
attributes.value: Dgcr8 gt2/tm1