title: GSM4162238: HCC8; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using miRNeasy kits (Qiagen) according to manufacturer instructions. Samples were stored in aliquot at – 80 °C prior to use. All reagents, plastic ware, and supplies used were sterile, nuclease free, and of molecular biology grade. RNA purity (A260/A280>1.80) and concentration were determined using nanodrop spectrophotometer (Thermo Fisher scientific, Delaware USA). RNA integrity was studied using the 2100 Bioanalyzer System (Agilent) and RNA 6000 Nano Kit. A RNA Integrity Number (RIN) of >7 was considered the lowest cut-off for RNA Sequencing. Sequencing library were constructed using Illumina's TruSeq Stranded total RNA Library Preparation Kit.
attributes.tag: GEO Accession
attributes.value: GSM4162238