title: Transcriptome of Gastric cancer cell line ACP02
design: The Charge Switch Total RNA Cell kit (Life Technologies, EUA) was used to isolate total RNA from ACP02 cells according to the manufacturer's protocol. The quality and quantity of the RNA were evaluated through on agarose gel and with Qbit system (Life Technologies, USA), respectively. Ribosomal RNAs were removed using RiboMinus ™ transcriptome Isolation Kit (Human / Mouse) from Life Technologies. At depleted RNA total was added RNA ERCC92 spike in (Life Technologies) internal calibrator in the ratio of 1 μl of the diluted 1/167 spike in RNA per 300 ng from depleted RNA total.  SOLiD cDNA libraries were constructed using SOLiD Total RNA-Seq kit (Life technologies, EUA) from 300 ng of rRNA depleted total RNA and with ERCC92 calibrator. All steps for obtaining the libraries were carried out by the robot AB Library Builder System (Life Technologies, USA).  For the SOLiD sequencing (Solid 5500Xl system), the cDNA molecules were fixed in microparticles (Ion Sphere) and amplified in emulsion PCR from 50 pg of each library using the SOLiD® EZ Bead System  and reagents from Total RNA-Seq Libraries kit (Life Technologies, USA). Each cDNA library was deposited in two cells from the slide SOLiD sequencer with a maximum capacity of 20 Gb of sequencing.