title: GSM4610392: J2CLIP-seq_siLuc_DMSO; Homo sapiens; RNA-Seq
library_construction_protocol: Staufen1 binding lncRNAs are enriched with Staufen1 antibody and extracted using Trizol reagent. TruSeq Stranded Total RNA Sample Prep Kit (Illumina, CA, USA) was used with 1 ug of total RNA for the construction of sequencing libraries. Total RNA was harvested using Trizol reagent. TruSeq Stranded Total RNA Library Prep Gold (Cat#20020598) was used with Total RNA 400ng that was subjected to ribosomal RNA depletion, with Ribo-zero Gold reagent for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4610392