title: NextSeq 500 sequencing; Rat (Rattus norvegicus) RNA-Seq containing SHAM, STROKE and MANF groups
design: Rat (Rattus norvegicus) RNA-Seq containing SHAM, STROKE and MANF groups
library_construction_protocol: RNA was extracted from punch-samples of tissue, taken from the lateral peri-infarct cortex on two 1 mm thick sections from positions A/P -0.9 to +0.1 and A/P +1.1 to +2.1.  RNA was extracted with Trizol reagent and treated with DNase (#1906, Ambion). Total RNA concentration and quality were determined with NanoDrop 1000 v3.7.1 and Agilent 2100 Bioanalyzer.  For RNA sequencing rRNA was removed and the sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat
attributes.tag: Experimental Factor: injury
attributes.value: sham
attributes.tag: Experimental Factor: genotype
attributes.value: wild type genotype