title: GSM3475478: 86_LN_Id3pos_eTR [lib16899]; Mus musculus; RNA-Seq
library_construction_protocol: CD4+ T cells were isolated using CD4 microbreads (Miltenyi) from either peripheral LNs or spleens of 3 littermate Id3-GFP x Foxp3-mRFP mice. Cells were sorted based on viability, CD4, CD44, CD62L, Id3-GFP and Foxp3-mRFP expression on a FACs Aria II (BD Biosciences). 500 cells were sorted directly into SMART-Seq v4 Ultra Low Input RNA Kit (Takara) lysis buffer and protocol followed to produce cDNA. Library construction was performed using a modified protocol of the NexteraXT DNA sample preparation kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3475478