title: NextSeq 500 sequencing; Estrogen receptor beta inhibits cholesterol biosynthesis through overexpression of mir-181a-5p in Triple Negative Breast Cancer
design: Estrogen receptor beta inhibits cholesterol biosynthesis through overexpression of mir-181a-5p in Triple Negative Breast Cancer
library_construction_protocol: The cell lines were purchased from ATCC (Rockville, USA) and were stained at 37°C in 5% CO2 -95% air humidified atmosphere. RNA was extracted using TRI Reagent® (Sigma-Aldrich). RNA concentration was assayed with a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific) and its quality assessed with the Agilent 4200 TapeStation with Agilent RNA ScreenTape Assay (Agilent Technologies). TruSeq smallRNA library Prep Kit (Illumina)
attributes.tag: Experimental Factor: phenotype
attributes.value: ER beta negative