title: A123-2.1 Individual 2 leaf 1
design: Genomic DNA was extracted using the CTAB method (HUANG et al. 2000). DNA quantity was checked with a NanoDrop spectrophotometer (NanoDrop products, Wilmington, DE, USA). Sequencing libraries for plants A123-1 and A123-2 were constructed using the Illumina TruSeq DNA Kit version C (Illumina Inc., San Diego, CA, USA) and quantified by Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Libraries were multiplexed to 10nM and were sequenced on a HiSeq2000 pair-end 100bp run in the UCR IIGB Genomics Core Facility (http://illumina.ucr.edu). The Illumina CASAVA 1.8.2 pipeline was used for base calling and de-multiplexing.  Barcode sequence was ATTCCT used for multiplexing