title: GSM3228386: Salt GMUCT Rep2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Control and salt treated plants were collected 4 hrs after first light. Leaves were immediately placed on liquid N2 and ground using a mortar and pestle. RNA was extracted using miRNeasy mini kit (Qiagen) according to manufacturers instructions. 2 rounds of PolyA+ selection was performed using the Dynabeads mRNADirect kit (Thermo) according to manufacturers instructions. 5' adapters were ligated directly this polyA+ selected mRNA as previously described (Willman et al, 2016). GMUCT libraries were generated as previously described (M. R. Willmann et al, 2016) where 5' adapters were ligated directly to polyA+ selected mRNA and downstream library preparation was also performed as previously described (M. R. Willmann et al, 2016).
attributes.tag: GEO Accession
attributes.value: GSM3228386