title: Copper treated mussel transcriptome
design: Specimens of Mytilus coruscus were collected from Qingbang island, Dongji, Zhoushan in Zhejiang province, China. Mussel were approximately 6 months old with an average weight of 30 gand an average body length of 11.5 cm. Before Cu2+ challenge, mussels were acclimated indoor with room temperature for 15 days. The treated mussels were cultured in the seawater with copper sulfate at 0.2 ppm.  All dissected tissues were flash-frozen in liquid nitrogen and then stored at -80oC prior to RNA extraction. Total RNAs were extracted by using the TRIZOL Kit (Invitorgen, Carlsbad, CA, USA) following manufacturerĄŚs instructions.