title: RNA-Seq of Seriatopora hystrix: Houbihu stable clone 3
design: RNA quantity and quality were assessed on a Nanodrop spectrophotometer (Infinigen) and a bioanalyzer (Agilent), respectively. RNAs were diluted to 20 ng/μl, and 50 μl (1 μg) of total RNA were used to prepare the TRU-Seq (Illumina) libraries as described previously (Mayfield et al. 2014a, d). Briefly, upon converting the RNAs to cDNA, each of the 12 samples was ligated to a unique adaptor such that all samples could be sequenced on the same flow cell of the Illumina Genome Analyzer (IIx) that was used for sequencing (100 base pairs [bp], paired end)