title: GSM2990903: 1394_48hr_1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells were grown in 50mL volumes to the indicated time point. 3mL of cells was taken and spun down for RNA acid-phenol extraction followed by standard etOH precipitation. A minimum of 5 ug of RNA was used as input for NEBNext® Poly-A enrichment and Ultra Directional II cDNA library construction. Poly-A enrichment coupled with directional cDNA dUTP second strand synthesis.
attributes.tag: GEO Accession
attributes.value: GSM2990903