title: GSM3230127: Irf1KO_D26_2; Mus musculus; RNA-Seq
library_construction_protocol: Distal colons were collected and frozen in liquid nitrogen. RNA was isolated from homogenized tissue with TRIzol reagent (Life Technologies) and then purified using an RNeasy Mini Kit (Qiagen) Following ribosomal RNA depletion, libraries were prepared using Kapa stranded RNA-seq kit and sequenced on an Illumina HiSeq 2500 to acquire 50 bp paired-end reads.
attributes.tag: GEO Accession
attributes.value: GSM3230127