title: Imperial College : B. dendrobatidis Illumina resequencing project (BGI) - LHURS8 (CH)
design: Genomic DNA was extracted and fragmented randomly and the required length DNA fragments were retained by electrophoresis. For this round of sequencing we used a 500bp insert size, with 100nt paired end reads.  First, the purified DNA sample was sheared into smaller fragments with a desired size by Covaris S/E210 or Bioruptor firstly. Then the overhangs resulting from fragmentation were converted into blunt ends using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an 'A' bases to the 3' end of the blunt DNA fragments, adapters were ligated to the ends and the fragments were enriched and amplified by PCR. The index tag was then introduced into the adapter at the PCR stage. Finally, cluster preparation and sequencing was performed using the Illumina HiSeq 2000 platform.