title: Ultra Low-Pass WGS of human cfDNA: Sample CRPC_1031_1
design: Blood was fractionated via sequential centrifugation, and plasma containing cfDNA was extracted using the QIAsymphony DSP Circulating DNA Kit (Qiagen, kit #937556). End repair, A-tailing, ligation of Illumina-compatible adapters and PCR amplification was performed on 3-20ng cfDNA using the Kapa Hyper Prep Kit (Kapa, kit #KK8504) to create sequencing libraries. 
attributes.tag: alignment_software
attributes.value: BWA-0.7.7-r441