title: 18S rRNA-seq water samples: Lake Cajititlan
design: DNA from the filters (20-25 m and 0.45 m) was extract using FastDNA Spin Kit for Soil (MP Biomedicals, OH, USA) and amplified using the V4 region of the 18S rRNA gene. The DNA products were purified using magnetic beads (AMPure XP - Beckman Coulter) and Indexed with Nextera XT kit (Illumina). The 96 samples were pooled at a concentration of 8 pM and loaded with 10% Phix control.