title: RNA-Seq of Zootermopsis nevadensis: alate female: Sample Znev_rAF2
design: "Total RNA was extracted from single individual termites in 1ml of Trizol(R) Reagent (Life Technologies) according to manufacturer's instructions.  The extracted RNA was suspended in 40 uL Nuclease Free Water (Ambion) and was treated with TURBO DNA-free(TM) kit (Ambion) to remove the genomic DNA contamination.  RNA concentration was measured by Qubit(R) 2.0 fluorometer (Life Technologies, Carlsbad) and RNA integrity was checked by Agilent RNA 600 nano kit (Bioanalyzer, Agilent Technologies). Bisulfite converted DNA and mRNA library preparation was performed in Georgia (http://dna.uga.edu), and sequencing was performed using the Illumina NextSeq500 platform."