title: Methylation profile of Vibrio cholerae strain
design: Both sides of DNA fragments were respectively connected with hairpin adapter to get a dumbbell (set of horse ring) structure, which was known as SMRTbell. After annealing, the SMRTbell was fixed at the bottom of the ZWM polymerase and used for sequencing. SMRT sequencing was carried out on the PacBioRS (Pacific Biosciences; Menlo Park, CA, USA) using standard protocols.