title: NextSeq 500 sequencing; RNA-seq experiment of developing mouse forelimbs, comparing Bcl9/9l mutant with control littermates.
design: RNA-seq experiment of developing mouse forelimbs, comparing Bcl9/9l mutant with control littermates.
library_construction_protocol: Forelimbs were manually dissected from three individual Bcl9/9l-deltaHD1/deltaHD2 mutants and three control littermates, at 10.5 days post coitum. Total RNAs from cells were extracted using TRIzol (Thermo Fisher Scientific, Belmont, Massachusetts, US) following the manufactured instructions. Sequencing Libraries were prepared with TruSeq RNA stranded library preparation.
attributes.tag: Experimental Factor: genotype
attributes.value: wild type genotype