title: GSM3533494: Sample_72N; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA samples of three pairs of Clear Cell Renal cell carcinoma and adjacent normal tissues were treated with the RiboZero rRNA Removal Kit and RNase R for deleting rRNA and linear RNA, according to the manufacturer's instructions.  The rRNA depleted and RNase R digested RNA samples were fragmented and synthesized cDNA with random primer. Purified the PCR amplification products of cDNA, then the libraries were quality controlled and sequenced by HiSeq2500 (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols, total RNA with strand-specific, rRNA-depleted
attributes.tag: GEO Accession
attributes.value: GSM3533494