title: GSM3230986: 50mM As(V) exposure; Lysinibacillus sp. B2A1; RNA-Seq
library_construction_protocol: Total RNA was extracted by Trizol® Reagent (Invitrogen, USA) according to the instruction manual. RNA purified was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology,USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 labchip kit (Agilent Technologies, USA). All RNA Sample preparation procedures were carried out according to the official Illumina protocol. Agilent's SureSelect Strand Specific RNA Library Preparation Kitwere used for library construction followed by AMPure XP Beads size selection. The sequence was directly determined using sequencing-by-synthesis technology. Sequencing data were generated by Welgene's pinepline which based on Illumina bcl2fastq v2.1.7 and expected to generate.
attributes.tag: GEO Accession
attributes.value: GSM3230986