title: WGS of mouse; adult male liver; offspring of C3H/HeN dam and C57BL/6N sire
design: Frozen liver tissues were quickly pulverized with BioPulverizer (Bio Spec Products Inc.) on dry ice. DNA was extracted with Allprep DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturer's protocol then further purified by phenol-chloroform extraction followed by ethanol precipitation.  DNA (5 g) was sheared using a Covaris E210.  After clean up with QIAquick PCR Purification Kit, the DNA was size-selected on a 1.5% Pippin Prep Cassette (Sage Science) (300-600bp broad range) and then cleaned up as before.  Illumina-compatible libraries were constructed on a SPRI-TE Robot (Beckman Coulter) using SPRI-TE reagents and paired-end adapters from Illumina.  Libraries were amplified using from 8-10 cycles and then cleaned up with two rounds of AMPure XP Bead clean up.