title: proband
design: The Nextera  Exome Enrichment Kit (Illumina Inc., San Diego, USA) was utilized to construct the libraries, which were prepared over a target region of 62 Mb, 20,794 genes, 201,121 exons. The sequencing was performed using two technologies. In eight trios, Illumina HiScan SQ  (Illumina, Inc.) was utilized. In 27 other trios, HiSeq 2500  (Illumina, Inc.) was utilized. The reads were sequenced with the paired-end technique, producing in average 24 million reads per sample with length of 100bp, resulting in 2.4Gb sequenced per sample.