title: GSM3535195: WT_Input_2 (ChIP-seq); Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP experiment was performed using iDeal ChIP-seq kit for Transcription Factors (Diagenode SA, Belgium). Briefly, cells were fixed for 15 min at room temperature with 1% formaldehyde-containing medium. Nuclei were isolated and chromatin was sonicated in the mixture of shearing buffer and protease inhibitor cocktail to an average size of 220 bp. Sonicated chromatin was used for immunoprecipitation by incubation with anti-RUNX3 antibodies (ab11905, Abcam) (40 μg) overnight at 4ºC. One per cent of chromatin used for each ChIP reaction was kept as input DNA. Washed protein A-coated magnetic beads (120 μl) were added to each ChIP reaction and reactions were incubated 2 hr at 4ºC. The beads were then incubated in 400 μl elution buffer at 65ºC for 4 hr to elute immunoprecipitated materials. Libraries were prepared using DNA SMART ChIP-Seq Kit (Clontech Laboratories, USA), according to the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM3535195