title: GSM2212736: 8I leftcolon female Ulcerative Colitis S115; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was prepared using the KAPA Stranded RNA-Seq with RiboErase sample preparation kit (kapabiosystems) in accordance with the manufacturer’s instructions. Briefly, 500ng of total RNA was ribo depleted, fragmented and went under first and second strand synthesis, A tailing, adapter ligation and PCR amplification (using 11 cycles).  Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Qubit Fluorometer (Life Technologies) and Agilent 2100 BioAnalyzer. Samples were sequenced on an Illumina HiSeq2500 sequencer (v4 chemistry) using 1 x 100bp cycles.
attributes.tag: GEO Accession
attributes.value: GSM2212736