title: Amplicon library of Neuro2a: predicted genes pooled shRNA transfected into N2a cells: Sample predicted genes' shRNA pool N2a (TaxId: 10090)
design: Custom primers amplified the shRNA sequence off a pLKO plasmid/genomic DNA and attach Illumina MiSeq adapters onto both ends. 3 technical replicates (from unique aliquots of genomic DNA) per n2a sample and 3 technical replicates for the injected shRNA pool