title: GSM4273675: 10X_Etv2_homozygous_20ss; Danio rerio; RNA-Seq
library_construction_protocol: Whole zebrafish embryos at 20ss were dissociated into a single cell suspension. GFP+ endothelial cells were sorted by FACS.Single-cell suspensions were processed through Chromium 10X to generate single-cell cDNA libraries attached to microbeads Microbeads were counted, and amplified by PCR, and the 3' end of the cDNA prepared for sequencing using a modified Nextera XT protocol
attributes.tag: GEO Accession
attributes.value: GSM4273675