title: Brachypodium distachyon spike rep 2 BS-seq
design: Three B. distachyon plants of the reference Bd21 line were grown under identical greenhouse conditions, including 20h days to induce rapid flowering. DNA was extracted from leaves at the beginning of anthesis.  Approximately two micrograms of genomic DNA was bisulfite treated using the MethylCode Kit (Invitrogen, Carlsbad, CA).   The NEBNext DNA Sample Prep Reagent Set 1 (New England Biolabs, Ipswich, MA) was used for library construction.