title: GSM1874103: RNAseq-Unstimulated-BV2; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq: Total RNA was extracted from the cells using Trizol (Invitrogen). The amount of total RNA was measured with a NanoDrop 2000 (Thermo Fisher Scientific, MA, USA) and a Qubit® RNA Assay Kit (Invitrogen,USA), respectively. RNA integrity was measured using the Agilent RNA 6000 Nano chip on a Bioanalyzer 2100 (Agilent, USA). RNA-seq: sequencing libraries were prepared from the extracted RNA  using NEBNext Ultra Directional RNA Library Prep (Cat. No.E7420L) according to the manufacturer's instructions. About 100–250 ng of total RNAs was used as starting materials, and library construction was done using distinct adapters for each library. From each library, equal volumes were pooled together and sequenced in a Illumina platform generating 76-bp tags
attributes.tag: GEO Accession
attributes.value: GSM1874103