title: Illumina HiSeq 2000 paired end sequencing; Gene expression of humanised keratin 12 in mouse cornea. A comparison between wild-type, heterozygous and homozygous genotypes.
design: Gene expression of humanised keratin 12 in mouse cornea. A comparison between wild-type, heterozygous and homozygous genotypes.
library_construction_protocol: Mice were sacrificed by CO2 asphyxiation Full thickness corneas were dissected, snap frozen and stored at -80Â°C RNA from frozen corneas was extracted after douncing and lysis using the RNeasy Plus Minikit (Qiagen) Illumina TruSeqRNA v2 with polyA capture
attributes.tag: Experimental Factor: humanised KRT12 homozygote
attributes.value: genotype