title: Illumina reads for P. yezoensis cDNA
design: First-strand cDNA was synthesized to selectively enrich full-length cDNAs with a cap structure and poly-A tail. The cDNAs were amplified by PCR using a proofreading DNA polymerase (KOD-Plus-Neo; Toyobo, Osaka, Japan). The primers used for the PCR were the GeneRacer 5' primer (5'-CGACTGGAGCACGAGGACACTGA-3') and the GeneRacer 3' Primer (5'-GCTGTCAACGATACGCTACGTAACG-3').