title: Illumina HiSeq 2000 paired end sequencing; Transcriptome analysis of 94 RILs of a maize MAGIC RIL population
design: Transcriptome analysis of 94 RILs of a maize MAGIC RIL population
library_construction_protocol: We sampled the first 0.5cm of the most basal part of leaf 4 3 days after appearance, always at the same time of the day to minimize diurnal effects All traits were measured in a series of experiments in a single growth chamber. RILs were grown in a randomized design each time along with their respective parents. Experiments were conducted under controlled growth chamber conditions (24°C, 55% relative humidity, light intensity of 170mmol m-2 s-1 photosynthetic active radiation, in a 16h/8h day-night cycle).  Total RNA was extracted using Trizol (Invitrogen) according to manufacturer’s instructions, followed by DNA digestion using the RNase-free DNase I kit (Qiagen).  Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, polyA containing mRNA molecules are reverse transcribed, double-stranded cDNA is generated and adapters are ligated.
attributes.tag: Experimental Factor: RIL94
attributes.value: individual