title: GSM4116098: Sample 3_vehicle; Mus musculus; RNA-Seq
library_construction_protocol: Trigeminal ganglia were homogenized in the RLT buffer using a Dounce homogenizer. Then, total mRNA was extracted using a RNeasy mini kit (Qiagen, Germantown, MD, USA). RNA sequencing was performed using the adapted Smart-seq2 protocol. Briefly, one ng of total RNA was reverse transcribed with the Oligo-dT30VN and template-switching oligo (TSO) primers. The total cDNAs were amplified using ISPCR primers and the library was made using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM4116098