title: GSM2811302: Lin-/cKit+/eYFP+ cells from whole bone marrow in vivo tamoxifen treated hScl-CreERT R26eYFP Srsf2 +/+_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from Lin-/cKit+/eYFP+ (Immature haematopoietic multipotent progenitors) cells from whole bone marrow of 3 replicates from Srsf2+/+ and Srsf2 P95H/+ mice post tamixofen using the Direct-zol RNA MicroPrep kit from Zymo Research. RNA was checked for quality using the LabChip GX Touch 24. 150ng total RNA was used as starting material. Post ribosome depleted RNA was purified and subjected to indexing and library preparation using the Kapa Stranded RNA-seq Library Preparation Kit for Illumina Platforms (Kapa Biosystems). Library amplification was performed for 13 cycles of PCR. The resultant libraries were pooled and sequenced on two lanes using the Illumina X-ten with 150bp paired end reads at  Novogene Bioinformatics Technology Co., Ltd, Hong Kong.
attributes.tag: GEO Accession
attributes.value: GSM2811302