title: RNA-seq of juvenile Pogonichthys macrolepidotus: Gill tissue
design: Gill tissue from each fish was homogenized using a TissueLyser (Qiagen) in Buffer RLT Plus (RNeasy Plus Mini Kit; Qiagen) and 350 ?l of the homogenate was used for the RNA extraction on a Qiacube (Qiagen) following manufacturers protocols. RNA quality was assessed using a Bioanalyzer (Agilent). Total RNA was used to prepare 32 separate cDNA libraries using KAPA Stranded mRNA-Seq Kits (KAPA Biosystems) barcoded with a unique adapter prior to sequencing. All libraries were pooled and sequenced on an Illumina HiSeq 2000 over six lanes. Sequencing was conducted to produce 100 base pair paired-end reads for each individual.