title: 16S sablefish Mar 4, 2016: Hatching silo E
design: DNA was extracted using the Qiagen DNeasy PowerSoil kit and used for an initial PCR amplification of the 16S rRNA gene (V3-4) with primers 341F/806R and Fluidigm CS1/CS2 linkers. A second PCR was run to include barcodes and sequencing adaptors (Fluidigm Access Array Barcode Library for Illuimina Sequencers). Final PCR products were pair-end sequenced (2 x 300 bp) on an Illumina MiSeq.