title: GSM2124942: FC_120_ck3; Mus musculus; RNA-Seq
library_construction_protocol: Freshly dissected tissue was flash-frozen in liquid nitrogen and frozen at -80° C until RNA could be extracted. Total RNA was disrupted in Trizol (Life Technologies, Carlsbad, CA, USA) with a mechanical pestle according manufacturer’s specifications. The aqueous phase was precipitated in isopropanol, resuspended in nuclease-free water, treated with DNase I (New England Biolabs, Ipswich, MA, USA), and cleaned up using a Zymo RNA Clean & Concentrator™-25 kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s specifications. Prior to library preparation, total RNA was checked for purity using a NanoDrop ND-1000 spectrophotometer, integrity using RNA Nano chips on an Agilent 2100 Bioanalyzer, and concentration using a Qubit fluormeter. RNA-Seq libraries were prepared from total RNA robotically using TruSeq Stranded mRNA HT (Illumina, San Diego, CA, USA) on an epMotion 5075 robot (Eppendorf, Hamburg, Germany). Single-end reads.
attributes.tag: GEO Accession
attributes.value: GSM2124942