title: ID8-G7 transcriptome
design: After RNA extraction, mRNA was enriched using Miltenyi Biotec µMACS mRNA Isolation Kit (Cat# 130-090-276) and converted to cDNA using Invitrogen Superscript Double-Stranded cDNA Synthesis Kit (Cat# 11917-010). cDNA was sheared, end polished, and ligated to adapters followed by 100bp paired-end sequencing on the Illumina HiSeq2000 platform.