title: Mixed DNA Sequencing On Illumina to Assess Instrument Bias
design: Plasmodium falciparum 3D7, Escherichia coli MG1655 and Rhodobacter sphaeroides 2.4.1 were mixed equimolar amounts to create a DNA sample which spans the GC spectrum. Paired-end Illumina shotgun libraries were created using standard library construction conditions and enriched on a Mastercycler epgradient S thermocycler (Eppendorf ) with maximum ramp speed using various PCR conditions to offset instrument sequence bias associated with sample GC content. After sequencing on a Illumina Hi-Seq with 101b PE reads, samples were analyzed for GC bias.
attributes.tag: analysis_type
attributes.value: AssemblyWithReference
attributes.tag: gssr_id
attributes.value: 67196.0
attributes.tag: library_type
attributes.value: WholeGenomeShotgun
attributes.tag: lsid
attributes.value: BROAD:SEQUENCING_SAMPLE:67196.0
attributes.tag: material_type
attributes.value: New Tech Library
attributes.tag: project
attributes.value: G2903
attributes.tag: work_request
attributes.value: 23058