title: GSM1923382: Glu_rho0_cytoRP_20; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: sample processing: RNaseI treatment, ribosome isolation by pelleting through 1M sucrose cushion, RNA isolation by phenol/chloroform extraction, gel purification of 27-33 nt RNA fragments Gel-purified RNA was treated with PNK and ligated to a pre-adenylated adapter using T4 RNA ligase 2, K227Q, truncated. Superscript III was used for reverse transcription and circ ligase for circularization. Illumina adapters were introduced by 10-14 cycles of PCR.
attributes.tag: GEO Accession
attributes.value: GSM1923382