title: GSM4273536: R variant of p53 from subject c4, untreated, replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted with Trizol (Invitrogen) and subsequently cleaned and DNase-treated using RNeasy columns (Qiagen). Libraries for RNA-seq were prepared with Lexogen QuantSeq 3'mRNA-Seq library prep kit at the Wistar Institute Genomics Facility and subjected to a 75 bp single-end sequencing run on NextSeq 500, using Illumina's NextSeq 500 high output sequencing kit following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4273536