title: GSM3230533: Sf3b1WT-2; Mus musculus; RNA-Seq
library_construction_protocol: Splenic B cells (B220+CD3-Gr1-Mac1-Ter119- cells) were FACS-sorted from recipient mice of bone marrow cells (that were harvested from primary mice with indicated genotypes) and RNA was extracted by using Qiagen Rneasy mini kit. Poly(A)-selected, unstranded Illumina libraries were prepared with a modified TruSeq protocol. 0.5X AMPure XP beads were added to the sample library to select for fragments <400 bp, followed by 1X beads to select for fragments >100 bp. These fragments were then amplified with PCR (15 cycles) and separated by gel electrophoresis (2% agarose). 300-bp DNA fragments were isolated and sequenced on an Illumina HiSeq 2000 (~100M 101 bp reads per sample).
attributes.tag: GEO Accession
attributes.value: GSM3230533