title: GSM3231518: PE-M_rep5; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA samples were isolated from P0-HUVECs using an RNeasy Mini Kit (Qiagen, Valencia, CA). The concentration and quality of each RNA sample were assessed using NanoDrop™ND-2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and Agilent 2100-bioanalyzer (Agilent Technologies, Santa Clara, CA). Only RNA samples with high RNA integrity number (>8.5) were utilized in this study. RNA libraries (one library per cell preparation) are generated using the Illumina TruSeq RNA Sample Preparation Kit (Illumina Inc., San Diego, California, USA) following the manufacturer's instruction. In brief, 300ng total RNA of each sample is fragmented using divalent cations under elevated temperature, and purified. Double-stranded complementary DNAs (cDNAs) are synthesized using SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, California, USA) and random primers. The cDNAs are ligated to Illumina adapters and amplified in a Linker Mediated PCR reaction (LM-PCR) for 9 cycles using PhusionTM DNA Polymerase and Illumina's PE genomic DNA primer set followed by purification (Agencourt AMPure XP beads, Qiagen).
attributes.tag: GEO Accession
attributes.value: GSM3231518