title: Sanger_zebrafish_sequencing
design: Sanger_zebrafish_sequencing
library_construction_protocol: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at -70 C Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions. Pellets were re-suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND-1000 Spectrophotometer (Axon Instruments).
attributes.tag: Experimental Factor: ORGANISM_PART
attributes.value: heart