title: GSM4273393: Chx10-Cre Rb1/p107-1 [tumor RNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNA Micro Kit (Qiagen). 500 ng of total RNA was used to create the RNA-seq library following the manufacturer's protocol from purification, mRNA fragmentation through the adenylation of end-repaired cDNA fragments and cleanup (TruSeq Stranded mRNA, Illumina). The collected sample was cleaned with AMPure XP beads (Beckman Coulter) and eluted in 20 μl of 10 mM Tris buffer, pH 8, 0.1% Tween 20.500 ng of total RNA was used to create the RNA-seq library following the manufacturer's protocol from purification, mRNA
attributes.tag: GEO Accession
attributes.value: GSM4273393