title: 16SrRNA-Seq of Sarshi Soil:Control
design: DNA was amplified and sequenced on Illumina MiSeq platform a. The V3 PCR primers were used.  PCR products were purified using InvitrogenTM PureLinkTM Quick Gel Extraction and PCR Purification Combo Kit. The PCR products were quantified using the Qubit 2.0 Fluorometer. The NEBNext Ultra DNA Library Prep Kit for Illumina was used to prepare the DNA library according to the manufacturer's instructions. Briefly, the steps involved end repair and dA tailing followed by adapter ligation. Clean up of adaptor ligated dNA was then performed using AMPure XP beads. The adaptor ligated DNA was then enriched by PCR (conditions described earlier). The PCR products were then cleaned using AMPure XP beads and size distribution of the library confirmed by running it on a Agilent Bioanalyzer