title: HaloTag DAP-seq control
design: Genomic DNA from Eucalyptus grandis (TAG0014 clone) was extracted using the NucleoSpin Plant Kit (Machery-Nagel). The sample (5-10 g in 130 L elution buffer: 10mM Tris-Cl, pH 8.5) was fragmented to ~200 bp using a Covaris S2 sonicator. Partial library adaptor ligation, in vitro protein expression, in vitro DNA binding and library preparation was performed as described by Bartlett et al. (2017,  doi:10.1038/nprot.2017.055).