title: AB SOLiD 4 System sequencing; Foxc1 IIBA ChIP-seq
design: Foxc1 IIBA ChIP-seq
library_construction_protocol: The preparation of the material was as described in Kutejova et al., 2008. Second branchial arches are dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. MinElute PCR purification kit (Qiagen) ChIP-seq was performed as described in Donaldson et al., 2012, using 20 ug of Foxc1 antibody (Abcam). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001.
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: input DNA