title: GSM3229722: T2_23h_42.77oxygen_A; Mycobacterium tuberculosis; RNA-Seq
library_construction_protocol: Biological triplicate cultures (A-C) of M. tuberculosis H37Rv wildtype in 7H9 media, were grown as 750 mL cultures in 2L spinner flasks, at 37ºC, 5%CO2. Mass flow controllers were used to adjust the amount of nitrogen and air flowing into the headspace. Sample were collected via syringe through sampling port. After harvesting, cells were spun down, flash frozen as cell pellets and kept frozen at -80 until RNA isolation. Cell pellets were resuspended in lysing buffer and transferred to a tube containing Lysing Matrix B, and vigorously shaken at max speed for 30 s in a FastPrep 120 homogenizer three times. This mixture was centrifuged at max speed for 1 min and the supernatant was transferred to a fresh tube. RNA was isolated using chloroform/isopropanol extraction with DNase treatment. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3229722