title: GSM3229671: TAIL-seq exp #6 siCont; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted from HeLa cells by TRIzol reagent (Invitrogen, 15596-018) and purified by RNeasy MinElute column (Qiagen, 74204) according to manufacturer's instruction. TAIL-seq was carried out as described previously (Chang et al., 2014b). Briefly, 25-50  ug of total RNAs (>200 nt) were extracted by TRIzol (Invitrogen), purified by RNeasy  MinElute column (Qiagen), and rRNA-depleted by using Ribo-zero kit (Epicentre). The  RNAs were ligated to the biotinylated 3′ adaptor, and partially digested by RNase T1.  The fragmented RNAs were pull-downed with streptavidin beads, phosphorylated at the  5′ end, and gel purified (500-1000 nt). The purified RNAs were ligated to the 5′ adaptor, reverse-transcribed, and amplified by PCR.
attributes.tag: GEO Accession
attributes.value: GSM3229671