title: GSM4272806: KO1_1; Mus musculus; RNA-Seq
library_construction_protocol: Lymphoid CD4 positive T cells were sorted by FACS. RNA was isolated from the T cell pellet by Trizol Reagent (Invitrogen), and the quality was determined by Bioanalyzer 2100 (Agilent). The 23S and 16S rRNA were depleted with MicrobExpress kit (Ambion). Genomic DNA was removed by two digestions using Amplification grade DNAse 1 (Invitrogen). The RNA was sheared and reverse transcribed by random primers to obtain cDNA. The library quality was determined by Bioanalyzer 2100 (Agilent).RNA libraries were prepared for sequencing using MGIEasymRNA Library Prep Kit V2. Then, the library was used for sequencing including making DNA NanoBalls (DNBs).
attributes.tag: GEO Accession
attributes.value: GSM4272806