title: WGS of Nocardioides sp. S5
design: DNA was extracted and purified using Wizard genomic DNA purification kit (Promega) following manufacturers instructions. Sequencing with a V3 chemistry 600-cycle kit (Illumina), was done using a MiSeq sequencer with PCR-free pair-end (PE) and mate-pair (MP) libraries that were prepared with TruSeq DNA PCR-Free and Nextera Mate-Pair library preparation kits (Illumina), respectively, following the manufacturers instructions. Obtained reads were processed with ShortReadManager 0.995 to extract pair reads, perform low-abundance 21-mer read trimming and discard reads shorter than 150 and 100 bp in PE and MP datasets, respectively. One million PE (281 Mb) and 0.9 million MP (160 Mb) reads were assembled with Newbler 2.8 (Roche).