title: gut 16S sequencing
design: A region of the 16S rRNA gene was PCR amplified using derivatives of primers 27F/338R, which were modified to add a unique sequence tag to the amplicons from each sample in a pool as listed. Amplicons were quantified by picogreen and pooled to a final concentration of 100 ng per amplicon.
attributes.tag: library strategy
attributes.value: targeted locus
attributes.tag: gene
attributes.value: 16s rRNA V2 region