title: RNA Seq from wild-type HEK293T cells
design: DNase-treated RNA was extracted by Sowd et al. (PNAS. 2016) from wild-type HEK293T cells using the Quick-RNA Miniprep kit (Zymo Research).  RNA was polyA selected, fragmented, reverse transcribed, and ligated to sequencing adapters using the TruSeq Stranded mRNA Library prep kit (Illumina). Single end 75 bp sequencing was conducted by the Dana-Farber Molecular Biology Core Facilities using an Illumina NextSeq500.