title: scAAV-CB6-PI-EGFP
design: HindIII digestion to remove wtTRs followed by gel purification.  DNA from purified rAAV viral vector preparations were treated with PreCR® Repair Mix (NEB, # M0309L) for nicked and end repair before directly ligating to SMRT-Bell adapters at a 1:1, adapter to vector molecular ratio and sequenced on a Pacific Biosciences RSII Instrument