title: GSM4164815: PATU8902_DMSO_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: PATU-8988T and PATU-8902 cells were treated with DMSO or 2.5 μM DCLK1-IN-1, washed twice with PBS, lysed and homogenized using Qiashredder columns (Qiagen). RNA extraction was performed using the RNeasy Plus Mini Kit (Qiagen) according to manufacturer's instructions. Prior to library preparation, ERCC RNA Spike-In Mix #1 (Life Technologies) was added to cell-count-normalized RNA samples. Illumina sequencing libraries were prepared using the KAPA mRNA HyperPrep kit (Roche). Equimolar multiplexed libraries were sequenced on a NextSeq 500 (Illumina, 75 bp single-end reads).
attributes.tag: GEO Accession
attributes.value: GSM4164815