title: GSM3231048: M2B_WT_SELEXseq_R1; Homo sapiens; OTHER
library_construction_protocol: Protein-DNA binding reactions were loaded onto a polyacrylamide gel and subject to electrophoretic mobility assay (EMSA), allowing for separation of bound and unbound fragments. Bound fragments were isolated from the gel and subject to phenol:chloroform extraction for DNA extraction, followed by ethanol precipitation. Libraries were prepared for sequencing based on the Illumina TruSeq small RNA kit sequences. The DNA library 5'-GAGTTCTACAGTCCGACGATCCGC[N16]CCTGGAATTCTCGGGTGCCA-3' containing  fixed regions compatible with Illumina sequencing was used in a 4-cycle PCR with primers RP1 5'AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA  and RPI# 5'CAAGCAGAAGACGGCATACGAGAT[Index]GTGACTGGAGTTCCTTGGCACCCGAGAATTCCA to add final Illumina adapter regions and indexes for sample multiplexing.
attributes.tag: GEO Accession
attributes.value: GSM3231048