title: GSM3074565: nTg-120d-3; Mus musculus; RNA-Seq
library_construction_protocol: For RNA extraction cervical sections of spinal cord were isolated from control group (30-60 days), presymptomatic (90 days) and symptomatic (120 days) heterozygous animals and age mice matched wild-type control animals. Each experimental group consisted five individuals with exception of presymptomatic group of DFUS(1-359) mice which consisted of four animals in total. Total RNA was extracted using Extract RNA reagent (Evrogen). Concentration of RNA was measured by Qubit Fluorometer (Invitrogen). The quality of RNA was determined by Agilent BioAnalyzer 2100 using RNA 600 nano kit. RNA Integrity Number (RIN) of all RNA samples was not less than 8 Libraries for RNA-seq were prepared using TruSeq RNA Library Preparation kit v2 (Illumina) according to the manufacturer's guidelines.
attributes.tag: GEO Accession
attributes.value: GSM3074565