title: RNA-seq of Zea mays : root: Sample WNII
design: The roots of mutant rtcs and wild-type in normal-N and low-N group were sampled and immediately frozen with liquid nitrogen at 12 h, 24 h, 48 h and 96 h after two N treatments, and labelled as RNI-IV, RLI-IV, WNI-IV and WLI-IV, successively. All samples were harvested from each of the three maize seedlings, and two independent replicates were collected for each sample.  Total RNA of the roots was isolated with Trizol reagent (Invitrogen, USA) according to the manufacturer’s protocol. The transcriptome sequencing library was generated using NEBNext Ultra RNA Library Prep Kits for Illumina (NEB, USA).125bp paired-end reads were generated using Illumina HiSeq 2500.