title: NextSeq 500 paired end sequencing
design: RNA extracted from cultered cells not infected by Nipah Virus for illumina sequencing (replicate 3)
library_construction_protocol: Nipah-infected and mock-infected Ptgv cells were collected for RNA-sequencing 24 hours post-infection in BSL4 Lyon.  The quantity of virus used for infection correspond to a MOI of 3 in VERO cell. RNA was extracted using NucleoSpin� RNA (MACHEREY-NAGEL, 740955). Libraries were generated using NEXTflex� Rapid Illumina Directional RNA-Seq Library Prep Kit according to manufacturer�s protocol (Bioo Scientific).Librairies were sequenced via Illumina NextSeq 500 in paired-end 75 bp using a Mid-Output Flow Cell.