title: RNAseq of Coryphaena hippurus: dispersant high 2
design: RNA was extracted from 15-30 larvae using the Qiagen RNEasy mini kit. Single-end sequencing libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and the resulting mRNA prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts). Libraries were sequenced on 1 lane of an Illumina NextSeq500