title: Pseudonaja textilis NGS de novo transcriptome
design: RNA was enriched for mRNA. The shot gun cDNA library was done using N6 random primers. The library was normalized to enrich with underrepresented transcripts by a thermal denaturation/renaturation step and further isolation of non-renaturated sscDNA by hydroxyapatite chromatography. Those sscDNA were double-stranded by PCR and sequenced by pyrosequencing.