title: Illumina HiSeq 2000 paired end sequencing; RNA-Seq of adipose tissue from mice infected with Trypanosoma brucei
design: RNA-Seq of adipose tissue from mice infected with Trypanosoma brucei
library_construction_protocol: Non-infected (naive) mice were sacrificed and perfused transcardially with pre-warmed heparinized saline (50 mL PBS with 250 l heparine per animal) using a peristaltic pump. Gonadal adipose tissue was collected and immediately lysed using TRIzol reagent. Total RNA was extracted using TRIzol reagent according to the manufacturers instructions (Life Technologies). Total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturers instructions (Invitrogen). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina).
attributes.tag: Experimental Factor: infect
attributes.value: none
attributes.tag: Experimental Factor: time
attributes.value: 0