title: GSM3140506: Rif1 SG replicate 2; Drosophila melanogaster; OTHER
library_construction_protocol: Salivary glands were extracted from wandering 3rd instar larvae. Ovaries were dissected from females fattened for 2 days on wet yeast and stage 13 egg chambers were isolated by hand dissection. Tissue was disrupted by douncing and then sonicated to fragment the genomic DNA. Genomic DNA extracted using a standard phenol chloroform extraction protocol. Illumina libraries were prepared using NEB DNA Ultra II (New England Biolabs) following the manufacturers protocol. Barcoded libraries were sequenced using Illumina NextSeq500 platform.
attributes.tag: GEO Accession
attributes.value: GSM3140506