title: Illumina HiSeq 2000 sequencing; RNA-seq of female mouse cerebral cortex with vehicle or 17-beta estradiol treatment
design: RNA-seq of female mouse cerebral cortex with vehicle or 17-beta estradiol treatment
library_construction_protocol: Silastic tubing containing 35 ul of  oil or 35ul of oil with 180ug/ml E2. Implants remained in mice for 7 days. Total RNA was extracted using Ambion RNAqueous  according to manufacturers protocol.Samples were treated with Turbo DNA-free reagent to remove genomic DNA. The TruSeq RNA sample prep kit was used according to manufacturers protocol. 1ug total RNA was used to make poly-A selected and barcoded libraries. Libraries were pooled and quantifed using real time PCR and library quantification kit (Kapa Biosystems).
attributes.tag: Experimental Factor: compound
attributes.value: 17beta-estradiol
attributes.tag: Experimental Factor: dose
attributes.value: 180 microgram per milliliter