title: GSM4273871: EGME_2; Rattus norvegicus; ncRNA-Seq
library_construction_protocol: The serum was centrifuged at 16,500 g for 20 min at 4°C, and the supernatant was obtained (pretreated serum). The pretreated serum (2.7 mL) was centrifuged at 208,000 g for 70 min at 4°C and the supernatant was discarded. The exosome pellet was resuspended in PBS and centrifuged again at 208,000 g for 70 min at 4°C. The supernatant was discarded, and then QIAzol Lysis Reagent were added to the exosome pellet for RNA extraction. The total RNA was extracted using miRNeasy Serum/Plasma Kit according to the manufacturer's protocol. cDNA libraries for small-RNA sequencing were synthesized with a NEBNext Multiplex Small RNA Library Prep Set for illumina and quantified with a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA) using Qubit dsDNA HS Assay Kit (Life Technologies) according to the manufacturer's protocol. Small-RNA sequencing was performed with an Illumina MiSeq System using MiSeq Reagent Kit v3. For the improved cluster formation, denatured PhiX Control v3 was added to the cDNA libraries.
attributes.tag: GEO Accession
attributes.value: GSM4273871