title: GSM4272579: HA_25417; Homo sapiens; RNA-Seq
library_construction_protocol: Bulk RNAseq iMg  Cells were washed twice with RT PBS, then lifted with StemPro accutase and spun down at 1,500 rpm for 5 min. Fresh cells were processed through Qiashredder and RNeasy mini kit  and frozen at -80°C.    Bulk RNAseq MDM  Cells were washed twice with RT PBS, then lifted with StemPro accutase and spun down at 1,500 rpm for 5 min. Fresh cells were processed through Qiashredder and RNeasy mini kit  and frozen at -80°C.    BulkRNAseq iAst  Cells were washed twice with RT PBS, then lifted with StemPro accutase and spun down at 1,500 rpm for 5 min. Fresh cells were processed through Qiashredder and RNeasy mini kit  and frozen at -80°C.    Bulk RNAseq human astrocytes  Cells were washed twice with RT PBS, then lifted with StemPro accutase and spun down at 1,500 rpm for 5 min. Fresh cells were processed through Qiashredder and RNeasy mini kit  and frozen at -80°C. Next-generation sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA library preparation kit, using high quality RNA (RIN >= 8.7) and 200ng of input. Libraries were assessed for quality using the PerkinElmer Labchip GX and qPCR using the Kapa Library Quantification Kit and the Life Technologies Viia7 Real-time PCR instrument. Libraries were diluted to 2nM and sequenced in a paired-end (2 x 100bp), dual-indexed format on the Illumina HiSeq2500 using the High Output v4 chemistry.
attributes.tag: GEO Accession
attributes.value: GSM4272579