title: GSM1679407: Male small RNA sequence; Toxocara canis; miRNA-Seq
library_construction_protocol: High-quality total RNA were extracted from the entire body of a male and a female adult worms using Trizol reagent (Invitrogen, Carlsbad, CA, USA), respectively. The RNA yields and qualities were measured spectrophotometrically (eppendorf BioPhotometer 6132). 20 μg RNA from the two samples were size fractionated with Novex 6% TBE-Urea gels (Invitrogen, Carlsbad, CA, USA) and, the fragments of 18-30 nt were ligated with 5' and 3' adaptors (Illumina) for reverse transcription. The produced first-stranded cDNA was amplified with small RNA primer set to enrich the libraries
attributes.tag: GEO Accession
attributes.value: GSM1679407