title: ddRADSeq of Peristenus
design: the enzyme pair NlaIII and MluCl (NEB, Ipswich, MA, USA) were chosen. Libraries were prepared on 48 individuals grouped by DNA yield, with each sample assigned one of 48 unique 5-base pair (bp) in-line barcode sequences during adapter ligation. Each set of 48 samples was then pooled for automated selection of a 216-336bp fragments on a PippinHT (Sage Science, Beverly, MA, USA), followed by 12 rounds of high-fidelity PCR amplification (Q5 High-Fidelity DNA Polymerase, NEB) using PCR primers that included one of 12 unique Illumina multiplex read indices.