title: Illumina HiSeq 4000 sequencing; Bulk RNA sequencing of colorectal tumors for molecular subtyping
design: Bulk RNA sequencing of colorectal tumors for molecular subtyping
library_construction_protocol: Resection samples were cut open and rinsed with water to remove any remaining stool. Two samples from each tumor specimen were collected corresponding to tumor core and invasive margin (tumor border). The samples were then snap frozen in liquid nitrogen and subsequently stored in -80oC for long term storage. RNA isolation was performed with Trizol (15596026, Invitrogen) using the protocol recommended by the manufacturer. For nucleic acid library construction, nKAPA Stranded mRNA-Seq Kit (KR0960 – v5.17, KAPABiosystems) was used according to the manufacturer recommendations.
attributes.tag: Experimental Factor: individual
attributes.value: SC029
attributes.tag: Experimental Factor: sampling site
attributes.value: tumor core