title: GSM1603862: Zfp217_ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: After cross-linking cells for 10 min with 1% formaldehyde and sonicating chromatin with a Diagenode Bioruptor, chromatin was immunoprecipitated using 10 µg of Zfp217 antibody (Santa Cruz Biotechnology, SC-55351x) for 10E6 cells. ChIP-DNA was end repaired with T4 DNA polymerase and polynucleotide kinase. An A-base was added to the end-repaired DNA fragments. Solexa adaptors were ligated to the ChIP DNA fragments and 200-300bp size fractions were excised from 2% agarose gel stained with GelStar. Adaptor-modified fragments were enriched by 16 cycles of PCR amplification. The DNA library prep was validated in Bioanalyzer for quantity and size.
attributes.tag: GEO Accession
attributes.value: GSM1603862