title: GSM1606634: e7.5 EPC Input (1); Mus musculus; ChIP-Seq
library_construction_protocol: Ectoplacental cone (EPC) tissue was dissected from embryos at e7.5 and e9.5, obtained from timed pregnant CD-1 mice (Charles Rivers Labs).   The EPC was separated from the maternal decidua, embryo, and other extraembryonic tissue.  Chromatin isolation and immunoprecipitation were carried out as previously described (Tuteja et al. 2008 Nucleic Acid Research 36(12): 4149-4157)  but with 5μg chromatin and 1.2μg of H3k27ac antibody (Abcam ab4729, lot: GR24371-1) for the immunoprecipitation assay. For each sample of immunoprecipitated DNA, 2ng were modified for sequencing following the Illumina's protocol (Preparing Sample for ChIP Sequencing of DNA (11257047 Rev A)).
attributes.tag: GEO Accession
attributes.value: GSM1606634