title: GSM4117188: Fraction_21P; Streptococcus pneumoniae; RNA-Seq
library_construction_protocol: Clarified lysates were centrifuged on a glycerol gradient, which was subsequently fractionated and the RNA extracted by P/C/I extraction. Input control was isolated using TRIzol reagent. RNA was fragmented, and the 3' TruSeq adapter ligated to the 3' end. Following cDNA synthesis, the 5' TruSeq adapter was ligated to the 3' end and the resulting cDNA amplified by PCR.
attributes.tag: GEO Accession
attributes.value: GSM4117188