title: Illumina HiSeq 4000 sequencing; Thyroid deficiency before birth modifies adipose transcriptome to promote overgrowth of white adipose tissue and impair thermogenic capacity
design: Thyroid deficiency before birth modifies adipose transcriptome to promote overgrowth of white adipose tissue and impair thermogenic capacity
library_construction_protocol: At 105-110 days of gestation (dGA: term ~145+/- 2 days) and under halothane anaesthesia (1.5% halothane in O2-N2O), twin fetuses of pregnant ewes underwent either a thyroidectomy (TX) or a sham operation in which the thyroid gland was exposed but not removed (sham). At either 129 or 143 dGA, the fetuses were delivered by Caesarean section under general anaethesia (20mg/kg maternal body weight sodium pentobarbitone I.V.). Blood samples were collected by venepuncture of the umbilical artery into EDTA-containing tubes. Each fetus was weighed and a variety of fetal organs, including perirenal adipose tissue, were collected after the administration of a lethal dose of barbiturate (200mg/kg sodium pentobarbitone I.V.).   Tissue samples were immediately frozen in liquid nitrogen and stored at -80C until analysis. RNeasy Lipid Tissue Minin Kit (Qiagen, Manchester, UK) cDNA libraries were prepared in samples with RIN>6 (Agilent bioanlyser)
attributes.tag: Experimental Factor: injury
attributes.value: thyroidectomy