title: GSM1606982: L7_FC819_Lane7_ED50_Inflorescence_HA-AGO2-DAD(ago2-1)_TuMV_IP; Arabidopsis thaliana; RIP-Seq
library_construction_protocol: Rosette leaves, cauline leaves or inflorescence tissue (1g) were collected from immunoprecipitation. Total RNA was extracted from input and immunoprecipitation fractions. Small RNAs were size fractionated from input samples. Total RNA was extracted from input fraction and small RNAs separated by size fractionation. Small RNAs in input and HA-AGO or HC-Pro immunoprecipitation fractions were converted to DNA Amplicons by 5' (GUUCAGAGUUCUACAGUCCGACGAUC) or  3’ (CTGTAGGCACCATCAAT) adaptor ligation followed by RT-PCR. DNA Amplicons were sequenced using the Illumina HiSeq2000 platform. Duplicate libraries were made per treatment. For each library, hits to TuMV, to TuMV-HIS and to Arabidopsis thaliana were included in separate files. Sequencing libraries were multyplexed using indexed 3' PCR primers.
attributes.tag: GEO Accession
attributes.value: GSM1606982