title: Illumina HiSeq 4000 sequencing; RNA-seq of WT, Mbd3-/- and rescue hiPSC during neuroectodermal differentiation and of WT and Mbd3-/- in mEpiSC during neuroectodermal differentiation
design: RNA-seq of WT, Mbd3-/- and rescue hiPSC during neuroectodermal differentiation and of WT and Mbd3-/- in mEpiSC during neuroectodermal differentiation
library_construction_protocol: Cells were grown in self-renewing or differentiation conditions for specific times and harvested directly in TRI Reagent. RNA made from samples in TRI Reagent using Direct-zol RNA Miniprep Kit (Zmyo Research) according to manufacturers protocol Library Selection: Poly(A)+, Library Strand: First Strand
attributes.tag: Experimental Factor: organism
attributes.value: Mus musculus
attributes.tag: Experimental Factor: genotype
attributes.value: wild type genotype
attributes.tag: Experimental Factor: time
attributes.value: 4