title: The microbial rare biosphere in a subtropical freshwater lake
design: Library Construction protocol:  Four environmentally-distinct surface water sites were sampled: two near-shore (littoral) stations (Lebanon Pool and Soldier Creek) and two off-shore (pelagic) stations (Red River and Buncombe Creek). We then sequenced 16S rRNA genes of bacterial assemblages using high-throughput sequencing (454/Roche Life Sciences GS-FLX Genome Sequencer)