title: RNA-seq of Candida orthopsilosis: replicate 1
design: Cells were grown overnight and diluted to an OD600 of 0.2 in 25 mL yeast peptone dextrose (YPD). Cells were grown at 30¡C  on a shaking incubator (200 rpm) for 3.5 hours. Cells were gathered by vacuum filtration. RNA was isolated using an Ambion RiboPure-Yeast RNA purification kit. Strand-specific RNA-seq libraries were prepared by BGI (www.genomics.cn, Hong-Kong) for sequencing using standard Illumina protocols.