title: GSM3533042: H3K27me3_M_EB45.1; Daphnia pulex; ChIP-Seq
library_construction_protocol: Daphnia samples (30 mg wet tissue per sample) were homogenised in 1 ml of PBS/1%formaldehyde using Dounce homogenizer. The chromatin immunoprecipitation sequencing libraries (ChIP-seq) were prepared using the iDeal-seq kit, H3K4me3 (C15410003-50, 1 ug/reaction), H3K27me3 (C15410195, 1 µg/reaction) antibodies and sequenced using Illumina HiSeq 2500 (1x50 bp) as part of a service provided by Diagenode (Belgium). Briefly, Daphnia samples (30 mg wet tissue per sample) were homogenised in 1 ml of PBS/1%formaldehyde using Dounce homogenizer. The collected cells were lysed and the nuclei were collected and sonicated to a final size of 80-400 bp. The mentioned antibodies were used to prepare test samples according to the manual for the iDeal ChIP-seq kit. The IP samples and input samples were quantified using the Qubit dsDNA HS kit. Library preparation was performed on the IP and input samples using the MicroPLEX library preparation protocol on 500 pg of DNA. The amplified libraries (13 PCR cycles) were purified using AMPure beads, quantified using the Qubit ds DNA HS kit and analysed on Bioanalyzer. The prepared libraries were then sequenced on HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM3533042