title: RNA-Seq library prepared from untreated larvae as a handling control
design: Short cDNA tags were produced for RNA-Seq using a custom protocol.  RNA was fragmented by incubation at high temperatures, and used to synthesize first strand cDNA with an anchored oligo-dT primer.  Sequencing adaptors were introduced during first-strand synthesis, and sample-specific barcodes during a subsequent PCR.  The final product was size-selected prior to sequencing to target a narrow window near the 3' end of each transcript.