title: RNA sequencing from C. lapponica that feed on birch including different stages: adults (male and female), pupae, first- to third-instar larvae and eggs .
design: Around 2.5 g of total RNA was then used for library preparation using TruSeq  RNA Sample Prep Kit v2 according to manufacturer s description. Pooled RNAs of different developmental stages from C.lapponica that feeds on birch were used for paired-end sequencing. Therefore, fragmentation step of these was shortened to four minutes (seven minutes for all the remaining samples) to obtain longer fragments. Libraries of pooled RNAs were sequenced in 100bp paired-end mode using sequencing chemistry TruSeq SBS Kit v3 (Illumina). The sequencing approach resulted in around 50 mio. read-pairs per sample. Reads were extracted in FastQ format using CASAVA v1.8.2 (Illumina) and used for further analysis.