title: Environmental 16S Metagenomic sequencing- L2
design: Genomic DNA extracted from 364 environmental samples, which included plant samples, soil samples, contaminated soil samples and continuous enrichment culture samples. The hypervariable V4-V5 region of the 16S rRNA gene was amplified using the primers F515 (5’-GTGCCAGCMGCCGCGGTAA-3’) and R926 (5’-CCGYCAATTYMTTTRAGTTT-3’), and pooled (2 pools) to sequencing on the MiSeq platform. Four libraries were constructed.