title: GSM1606671: E13.5 non-IPC(intermediate progenitor cell)-derived neurons; Mus musculus; RNA-Seq
library_construction_protocol: Heterozygous Tbr2(Eomes)-GFP mice were bred with homozygous DCX-mRFP mice to yield GFP/RFP double positive embryos. The cortices derived from the double positive embryos were dissociated by trituration in HBSS with 2mM of EDTA and 25 ug/ml of DNase I. Cells were washed once with HBSS and re-suspended in DMEM/F12. Fluorescence-activated cell sorting (FACS) was used to acquire intermediate progenitor cell-derived neurons (IPNs, double color) and non-intermediate progenitor cell-derived neurons (non-IPNs, red color). One million of IPNs and non-IPNs at embryonic day 15.5 (E15.5) was accumulated, respectively. Total RNA was extracted using TRIzol. For E13.5 and E16.5, 10000 IPNs or non-IPNs were collected and whole transcriptome was amplified by Ovation RNA-Seq System V2 kit (NuGEN) before sending to sequencing. Following Illumina's True-seq RNA-seq library prep kit
attributes.tag: GEO Accession
attributes.value: GSM1606671