title: Transcriptome response of EE2 in zebrafish
design: Library Construction: Illumina HiSeq 2000 protolcol.  Experimental design: Adult zebrafish and juvenile crucian carp (Carassius auratus) were bred and maintained in 10-L glass aquaria operating with a 14:10 h light:dark cycle at 23–26°C in the laboratory. Fish were acclimated for 1 week prior to use and fed with Tubifex worms once per day. Groups of adult zebrafish (9 male and 9 female) were exposed for 7 days to 50 ng/L (168.7 pmol/L) of 17α-ethinylestradiol (EE2; TCI chemicals, Tokyo, Japan), which had been diluted from a concentrated stock solution solubilized in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA). DMSO only was added to the water in control group tanks. Transcriptome response of EE2 in zebrafish liver were analysed.