title: GSM3380557: mESC ATAC-seq, biological replicate 2; Mus musculus; ATAC-seq
library_construction_protocol: 100,000 freshly harvested F123 cells were resuspend in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630) and rotate at 4oC for 15 minutes. After lysis the nuclei was spun down at 500×g for 5 min at 4 °C. Then the reaction was carried out for 30 min at 37 °C in 1×TD buffer with 2.5 μL transposase from Nextera DNA Library Prep Kit (Illumina). After reaction completion DNA is purified using MinElute PCR Purification Kit (Qiagen). PCR amplification was perform with 1×NEBNext PCR MasterMix and 1 μM i7-index and i5-index primers using the following PCR condition: 72 °C for 5 min; 98 °C for 30 s; and 8 cycles of 98 °C for 10 s, 63 °C for 30 s and 72 °C for 1 min. The amplified libraries are purified and size selected using 0.55× and 1.5× (total) of sample volume.
attributes.tag: GEO Accession
attributes.value: GSM3380557