title: Exosomal RNA from retinoblastomcontrolcell lines 3
design: 100 ng of Total RNA was used as the starting material. Adapters were ligated to 3' and 5' ends. The ligated products were reverse transcribed with Superscript III Reverse transcriptase. cDNA was enriched and barcoded by PCR (12 cycles) and cleaned using Polyacrylamide gel. The libraries were size selected in the range of 140  160 bp followed by overnight gel elution and salt precipitation using Glycogen, 3M Sodium Acetate and Absolute Ethanol. The prepared libraries were quantified using Qubit Fluorometer, and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip.