title: GSM4547619: R19028452LR01-22, Paracancerous benign tissue; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from formalin-fixed, paraffin-embedded tissue. After hematoxylin and eosin (H&E)-stained slide review, tumor and paracancerous benign tissues were selected. We then manually microdissected the corresponding tissue from unstained, 5-μm-thick tissue sections (10 sections for RNA). For RNA sequencing, we purified RNA using the RNeasy FFPE Kit from FFPE slides. RNA quality was determined with the DV200 value (DV200 > 30%) by a Caliper BioAnalyzer 2100 Instrument. RNA samples were submitted to WuXi NextCode for next-generation sequencing with TruSeq RNA Exome. Paired-end sequencing (2 × 150 bp reads) was performed on successful RNA libraries using the Illumina HiSeq X-Ten platform.
attributes.tag: GEO Accession
attributes.value: GSM4547619