title: GSM1606642: e7.5 EPC RNA-Seq (3); Mus musculus; RNA-Seq
library_construction_protocol: Ectoplacental cone (EPC) tissue was dissected from embryos at e7.5 and e9.5, obtained from timed pregnant CD-1 mice (Charles Rivers Labs).   The EPC was separated from the maternal decidua, embryo, and other extraembryonic tissue.  RNA was isolated using the Qiagen RNeasy Micro Kit, following the manufacturer’s protocol.  RNA quality was assessed using the Agilent Bioanalyzer, and all samples had a RIN score ≥ 8.7. mRNA was isolated and prepared for sequencing using Illumina’s TruSeq Stranded mRNA Sample Prep Kit, following the manufacturer’s protocol, and starting with 1ug total RNA.  6 libraries (3 biological replicates at each time point) were pooled and the pooled sample was run on two lanes (technical replicates).
attributes.tag: GEO Accession
attributes.value: GSM1606642