title: GSM2052588: SC3; Homo sapiens; RNA-Seq
library_construction_protocol: Primary human keratinocytes were established from 4-mm punch biopsies derived from non-lesional and lesional skin of patients with Hailey-Hailey disease. Cells were maintained in modified low calcium medium (Clonetics; Cambrex Bio Science, Walkersville, MD, U.S.A.). At passages 2 cell were used for study purposes. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2052588