title: GSM4547698: mutant 2; Danio rerio; RNA-Seq
library_construction_protocol: After euthanasia, the adult zebrafish were subjected to cardiac bleeding to remove most mature erythrocytes from the marrow. The zebrafish were then dissected at room temperature and the dissected marrow was placed into 500ul of 0.9x PBS with 2% FBS and 1 USP units/mL heparin, placed on ice. The marrow samples were then mechanically dissociated by repeated pipetting into single cell suspension and passed through a 40-μm nylon mesh. Part of the mutant marrow cell preparation was used for sorting the dominant color marrow cells on BD Aria 2 into 500ul 1x PBS. All 8 samples were washed by spinning down at 350 x g for 5 min at 4C. The cell pellelts were collected in 300ul of 1x DPBS with 0.04% BSA (Gemini Bio-Products), then washed again after spinning down at 350 x g for 5 minutes at 4°C and counted after resuspension in DPBS/BSA to target 10,000 cells per reaction. We followed the 10x genomics sample preparation protocol and the 10x genomics single cell RNA sequencing V2 library preparation single-cell RNA sequencing, 10x genomics
attributes.tag: GEO Accession
attributes.value: GSM4547698