title: next generation sequencing by using thorax tissue of apis mellifera ligustica
design: After the sample genomic DNA is qualified, the DNA is fragmented by mechanical disruption (ultrasound). Fragmented DNA is then subjected to fragment purification, end-repair, 3'-end plus A, ligation and sequencing adapters, followed by agarose gels. The size of the fragments was selected by electrophoresis, and PCR was used to form a sequencing library. The library was first subjected to library quality inspection. The qualified library was sequenced using Hiseq X Ten.
attributes.tag: Submission ID
attributes.value: SUB4199863