title: GSM2991385: IVBL rep8; Homo sapiens; RNA-Seq
library_construction_protocol: After homogenization, total RNA were extracted by Direct-zol RNA miniprep kit. . The first and second strand cDNA were prepared by SuperScript III First-Strand Synthesis System and NEBNext mRNA Second Strand Synthesis Module from at least 200ng total RNA for each sample. Illumina Nextera XT DNA Sample Prep Kit  was used with 1 ng of dsDNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using nextera Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2991385