title: RNA-Seq of eggplant
design: The mRNA was enriched by magnetic beads with Oligo (dT) and then the RNA fragments were reverse transcribed to double-strand cDNA (dscDNA) by N6 random primers. The synthesized cDNA was subjected to end-repair and then was 3 adenylated. Adaptors were ligated to the ends of these 3 adenylated cDNA fragments. The ligation products were purified and PCR amplification was performed to enrich the purified cDNA template using PCR primers. Lastly, the PCR products were denatured by heat and the single strand DNA was cyclized by splint oligo and DNA ligase.