title: human airway sample - ET133P
design: Amplicons were generated using primers that target approximately 300 base pairs of the V1/V2 variable region (27F/338R) of the 16S rRNA gene. PCR products were normalized using agarose gel densitometry, pooled, gel purified, and concentrated using a DNA Clean and Concentrator Kit (Zymo, Irvine, CA). Pooled amplicons was quantified using Qubit Fluorometer 2.0 (Invitrogen, Carlsbad, CA). The pool was diluted to 4nM and denatured with 0.2 N NaOH at room temperature. The denatured DNA was diluted to 20pM and spiked with 10% of the Illumina PhiX control DNA prior to loading the sequencer. Illumina paired-end sequencing was performed on the Miseq platform using a 500 cycle version 2 reagent kit.
attributes.tag: sample_ID
attributes.value: ET133P