title: MinION sequencing
design: DNA extracted from cultured cells for MinION sequencing
library_construction_protocol: High-molecular-weight DNA was extracted from cell culture using the MagAttract® HMW DNA Kit (Qiagen). Then, genomic DNA was sheared using Covaris g-TUBE as per manufacturer's guidelines to obtain approximately 10 kb fragments without any additional enrichment or purification step. DNA concentration was measured using the Quantus™ Fluorometer with the QuantiFluor® dsDNA Kit (Promega) and purity was evaluated by measuring A260/A280 and A260/A230 ratio using a NanoDrop™ spectrophotometer. The quality and the size of DNA was assess by electrophoretic migration using Fragment Analyzer with the High Sensitivity Large Fragment 50 Kb Analysis Kit (AATI). Sequencing libraries were prepared using the Ligation Sequencing Kit 1D (SQK-LSK108) or the 1D^2 Sequencing Kit (SQK-LSK308) (Oxford Nanopore Technologies) following the protocols supplied by the manufacturer. The libraries were then loaded to MinION using a flow cell R9.5 version (FLO-MIN106) (ONT) and the sequencing runs were performed under MinKNOW version 1.7.14 for up to 48h.