title: Illumina HiSeq 2500 sequencing; Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes
design: Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes
library_construction_protocol: LECs were isolated from inguinal LNs of C57Bl/6 wildtype mice. Cells were sorted into 384-well plates containing lysis buffer and RNA was extracted. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).
attributes.tag: Experimental Factor: single cell identifier
attributes.value: Inguinal_mLN_Cell_0005
attributes.tag: Experimental Factor: cluster
attributes.value: 1