title: GSM2991371: BRG1 binding in parental TOV21G cell line; Homo sapiens; ChIP-Seq
library_construction_protocol: Flag (Sigma, F1804) antibody was used on TOV21G and resistant endogenously Flag-tagged BRG1 cells. Chromatin were incubated overnight at 4°C and protein A+G Dynabeads were added to the reaction for another 1.5 hour. Magnetic beads were washed and chromatin was eluted and reversed. Chromatin was then treated with proteinase K and purified with Gel extraction kit (Qiagen, cat. no. 28706). ChIP DNA was used for ChIP-seq. For CHIP-seq NEBNext Ultra DNA Library Prep Kit (NEB, E7645) was used to prepare sequencing library
attributes.tag: GEO Accession
attributes.value: GSM2991371