title: FUS E18
design: FUS E18
library_construction_protocol: iCLIP protocol was followed to isolate RNA bound to FUS, TDP-43 or U2AF65 protein and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
attributes.tag: Experimental Factor: Immunoprecipitate
attributes.value: anti U2AF65 antibody