title: 16 S amplicon of microbiome of enriched panchagavya for plastic biodegradation
design: Nanopore sequencing was performed by using 1g of purified  DNA template on following sequence protocol.  End repair / dA tailing, Ligation of Barcode Adapter, Barcoding PCR , End repair/dA tailing, Blunt end Adapter Ligation, Purification using AMPure XP bead binding, Priming and loading the SpotON flow cell, EPI2ME 16S analysis workflow  to perform genus-level and species- level identification, The workflow is designed to BLAST basecalled sequence against the NCBI 16S bacterial database, which contains 16S sequences from different organisms. Each read is classified based on % coverage and identity