title: GSM4161683: PC3_Sen_88; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were trypsinized in 0.05% Trypsin EDTA for 5-10 mins at 37°C and washed with media.  The cell suspension was loaded into the Fluidigm C1TM machine and processed into single cell cDNA libraries according to the manufacturer protocol. Full length mtotal RNA-seq libraries were generated from single cells captured using the Fluidigm C1TM Single Cell mtotal RNA Seq IFC, 10-17 um and Fluidigm C1TM Single-Cell Reagent Kit for mtotal RNA Seq.  Full length cDNA was converted into sequence ready libraries using SMART-seq v4 Ultra Low Input total RNA kit for Fluidigm C1TM System and SeqAmpTM DNA Polymerase.  Library preparation was completed using Nextera XT DNA library prep kit.
attributes.tag: GEO Accession
attributes.value: GSM4161683