title: F.vescaH4x4gDNA
design: Genomic DNA library was prepared according to modified standard Illumina protocol (http://www.illumina.com/). Briefly, 7 ug of genomic DNA in a 50 uL of TE buffer (10 mM Tris, 1 mM EDTA; pH  8.0)  was mixed with 750 uL of nebulization buffer [25% (v/v) glycerol in TE buffer] and sheared for 7 min. in a nebulizer (Invitrogen) using compressed nitrogen at 36 psi. Before ligation with Illumina adapters the fragmented DNA was purified using "QIAquick PCR Purification Kit"(Qiagen). Illumina library size was in 300-350 bp range.