title: Asclepias syriaca (4885) Genome Sequence
design: A BioRuptor Sonicator was used to shear 2.5 micrograms of the extracted DNA using 15 one min cycles (30 sec on/30 sec off, setting high, 4 degrees C). Subsequent library preparation followed the standard multi-step Illumina protocol (Illumina 2006). Ligated, size-selected fragments (~280 - 320 bp) were amplified through 18 cycles of PCR, using Phusion High-Fidelity PCR Master Mix and standard Illumina primers.