title: GSM3380554: mESC H3K4me3 PLAC-seq, biological replicate 1; Mus musculus; OTHER
library_construction_protocol: In brief, 1-3 million crosslinked F123 cells were digested 2 hours at 37oC using 100 U of MboI followed by biotin fill-in and proximity ligation at room temperature for 4 hours. Then the nuclei were further lysed, sonicated and immunoprecipitated against the antibodies of choice. After immunoprecipitation, reverse crosslink was performed overnight at 65 oC after adding proteinase K to extract DNA. DNA fragments containing ligation junctions were enriched with streptavidin beads followed by on-beads end repair, A-tail adding, adapter ligation and PCR amplification for 12-13 cycles.
attributes.tag: GEO Accession
attributes.value: GSM3380554