title: Illumina MiSeq paired end sequencing
design: Described in Lluch et al “The Characterization of Novel Tissue Microbiota Using an Optimized 16S Metagenomic Sequencing Pipeline.” PLoS ONE 2015, 10(11)
library_construction_protocol: PCR amplification of the V3-V4 region of the 16S gene before Illumina library preparation