title: RNA-Seq of hirudo verbana muscle cell with normalization
design: Total RNA was extracted with ExtractRNA (Evrogen, Russia). The yield and purity were assessed using a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, USA), with the RNA integrity determined by the RNA Integrity Number (RIN) using a Bioanalyzer 2100 (Agilent Technologies, USA). The PCR-based cDNA library was created following the instructions for the Mint-2 cDNA synthesis kit (Evrogen, Russia). Adapters PlugOligo-3M and CDS-4M were used for cDNA synthesis. Normalization of cDNA was performed using the DNS (duplex-specific nuclease) in accordance with manufacturer protocol (Evrogen, Russia).