title: GSM1684570: PANC1 TCC; Homo sapiens; OTHER
library_construction_protocol: TCC library preparation is described in the paper (http://www.nature.com/nbt/journal/v30/n1/full/nbt.2057.html) Approximately 5 x 10^7 MCF7 and PANC1 cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature, crosslinking was quenched with 0.125 M glycine for 5 minutes at room temperature and cell pellets were collected and stored at -80°C. Nuclei were digested with 2000U HindIII prior to dilute solid-surface ligation reactions. For the ChIP-Seq, the anti-CTCF antibody was from Cell Signaling Technology CTCF (D31H2) XP® Lot 1, 3418S.
attributes.tag: GEO Accession
attributes.value: GSM1684570