title: S. mansoni strain GH2 genomic DNA sequencing
design: The Illumina TruSeq DNA sample preparation kit (FC-121-2001, Illumina Inc., San Diego, USA) was used according to the manufacturer's six-steps protocol: 1) DNA was sonicated by using a Bioruptor; 2) Extremities of sonicated DNA fragments were repaired ; 3) an A-base was added to these extremities ; 4) Universal (5'-AAT*GATACGGCGACCACCGAGATCTA*CACTCTTTCCCTACACGACGCTCTTCCGATCT-3’) and strain-specific (BRE: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC*CAATCTCGTATGCCGTCTTCTGCTTG-3’ ; GH2: 5’-GATCGGAAGAGCA*CACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG-3’) adapters were then ligated to these extremities ; 5) DNA products were migrated on agarose gel and 500bp  /-25 fragments were purified ; 6) PCR amplification to select only DNA fragments carrying adapters.