title: Illumina HiSeq 2500 sequencing; RNA-seq of human pluripotent cells with ancient ADSL
design: RNA-seq of human pluripotent cells with ancient ADSL
library_construction_protocol: Cells were fed with 3-ml fresh medium 24 hours before collection, and lyzed directly with lysis buffer RLT in Qiagen RNeasy Plus Mini Kit. Samples were stored at -80 degree before the RNA extraction. Pluripotent cells were cultured on the Matrigel-coated 6-well plate and fed with mTeSR1 medium. All cells were tested mycoplasma-free by PCR method. no additional treatment Total RNAs were extracted following the standard procedure provided by Qiagen RNeasy Plus Mini Kit, and quantified with Qubit™ RNA BR Assay Kit.  200-ng of initial RNA for each sample was used for RNA library preparation. RNA libraries of all samples were prepared by using the Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems with Human/Mouse/Rat rRNA Depletion Kit and then quantified for the fragmentation size and concentration with the Agilent High Sensitivity DNA Kit.
attributes.tag: Experimental Factor: genotype
attributes.value: ADSL.pV429A