title: WGS Achromobacter AR476-2
design: From 2 μg of purified DNA in 50 μl TE Buffer, DNA was fragmented by nebulization. Fragment ends were repaired, adapters were ligated, and fragments were gel purified and amplified, according to TruSeq® DNA Sample Preparation Guide Illumina (July 2012). The resulting library was checked in 2100 Bioanalyzer (Agilent Technologies) with the High Sensitivity DNA Kit, and included fragments with a size length range of 300-500 bp. Library was quantified by qPCR (Light Cycler 480 Roche) with Kapa Library  Quantification Kit. Concentration was normalized to 2 nM, and an equimolar pool was used to generate clusters in one lane. A paired-end run was performed (PE) 2x100bp