title: GSM2052623: Hippocampal CA1 CTRL1 RNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Rneasy Plus Kit (Qiagen). RNASEQ library was constructed using BIOO NEXTflexTM mRNA-Seq Library Prep Kit. Briefly, 1 mg of total mRNA was subject to a sequential workflow of poly-A purification, fragmentation,1st strand and 2nd strand synthesis, DNA end-adenylation, and adapter ligation. Finally, the libraries were enriched with 15 cycles of PCR reactions and cleaned with Agencourt AMPure XP magnetic beads (Beckman Coulter). The quality of prepared libraries was assessed using an Advanced Analytical-fragment Analyzer.  The bar-coded libraries were equally mixed for sequencing in a single lane on the Illumina HiSeq 2000 platform at the MIT BioMicro Center.
attributes.tag: GEO Accession
attributes.value: GSM2052623