title: GSM3142277: PVC_Kai1 KO; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from TRIZOL (Invitrogen) or RNeasy RNA isolation kit (Qiagen). Sequencing libraries were manually generated for high-throughput sequencing. Either end of DNA strands was filled using the Epicentre DNA END-Repair kit (Epicentre Biotechnologies, Madison, WI). Adenine was added at 3' ends by Taq DNA polymerase (New England Biolabs, Beverly, MA) under dATP conditions. Illumina's adaptor oligomers was ligated at both ends using Quick Ligation kit (Qiagen). After purified twice, DNA libraries are amplified using Solexa primers (Illumina, San Diego, CA) and Pusion PCR master mix (Thermo Scientific, Hudson, NH).
attributes.tag: GEO Accession
attributes.value: GSM3142277