title: GSM1604268: R14del-CMs (clone L2); Homo sapiens; OTHER
library_construction_protocol: Genomic DNA samples were assessed for quantity by Qubit fluorometry (Life Technologies, Grand Island, NY) and for quality by the 2100 Bioanalyzer system (Agilent). Initial shearing of 0.5–1 µg genomic DNA to an average of 200-300 bp fragments was performed using the Covaris E210 focused acoustic energy system (Covaris). Whole genome libraries were prepared using the NEBNext DNA Library Prep kit according to the standard manufacturer's protocol (New England Biolabs). Illumina compatible paired-end adapters were used and the adapter-ligated DNA fragments was amplified by ligation-mediated PCR (KAPA Biosystems) using a reverse PCR primer containing a six nucleotide barcode that allowed for multiples samples to be pooled and sequenced in the same run. The library was enriched for human exomic sequences using the SeqCap EZ Human Exome Library v3.0 capture system (Roche NimbleGen). The libraries were sequenced with a 100bp paired-end protocol on the Illumina HiSeq 2500 according to the manufacturer's protocol (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM1604268