title: GSM1606478: mixed sample of 7 and 14DPA grain; Triticum aestivum; OTHER
library_construction_protocol: Total RNA was extracted from the frozen 7, 14, 21 and 28 DPA grains using the ConcertTM Plant RNA Reagent (Invitrogen) according to the manufacturer’s instructions. Equal quantities (20 μg) of total RNA isolated from 7 and 14 DPA grains were mixed together to construct the degradome library I, and equal quantities of total RNA from 21 and 28 DPA grains were mixed together to construct the degradome library II. Degradome cDNA libraries using sliced ends of polyadenylated transcripts were constructed based on the method described previously (Addo-Quaye et al., 2008; Ma et al., 2010).
attributes.tag: GEO Accession
attributes.value: GSM1606478