title: Ion Torrent PGM sequencing
design: A PCR amplification step with a set of six custom BKPyV specific primers covering the complete genome was used to enrich for BKPyV from the urine sample.
library_construction_protocol: Libraries were prepared using the Ion Xpress Plus fragment library kit (Life Technologies). Libraries were amplified on the One Touch 2 with the Ion PGM Template OT2 400 Kit (Life Technologies), and sequenced on an Ion Torrent PGM using the 318 Chip Kitv2 and Ion PGM Hi-Q Sequencing Kit (Life Technologies).