title: RNA-Seq of Homarus americanus: motor neuron region of the cardiac ganglion
design: Double-stranded cDNA libraries were prepared from total RNA using a KAPA Stranded mRNA-seq kit (catalog No. KK8420; Kapa Biosystems, Wilmington, MA, USA) following the manufacturers instructions; 3 g of total RNA/sample was used for library generation. A Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA), AATI Fragment Analyzer (Advanced Analytical Technologies, Ankeny, IA, USA), and Kapa qPCR assays were used to determine the quality and quantity of the final pool of libraries.  Paired-end Illumina sequencing (150 base pairs [bp]) was performed on a NextSeq 500 system (Illumina, San Diego, CA, USA) using the high output kit v2 with 300 cycles.