title: Meis IBA ChIP-seq
design: Meis IBA ChIP-seq
library_construction_protocol: The preparation of the material was as described in Kutejova et al., 2008. First branchial arches are dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. ChIP assays were performed as described in Kutejova et al., 2008, with the following modifications for ChIP-seq. The crosslinked material was sonicated to 300 bp fragments (Vibracell sonicator, Sonics: 8 times for 10 seconds at 50% output). Immunoprecipitation was performed starting from 12 pairs of first branchial arches from E11.5 embryos and incubating overnight at 4oC with 3 ug of pan-Meis antibodies or control IgG (Kutejova et al., 2008), followed by protein A beads (Invitrogen). Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982.
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: input DNA