title: amplicon sequencing of fungi: grape must: Sample T0R2D1
design: "We sequenced fungal amplicons from fermenting grape must. Samples were taken from fermentation vats at different stages of fermentation, from grape must microcosms in which species richness had been manipulated, and from control communities with known numbers of fungal colony forming units. We amplified all samples using the fungal specific primers ITS7F and ITS4, and sequenced amplicons using the Illumina MiSeq platform."