title: GSM3473946: dTGM.1236; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA (5µg) was isolated from gastrocnemious muscles of 3 mice per group (ACTA1-MCM, FLExD, ACTA1-MCM; FLExD moderate TMX, and ACTA1-MCM; FLExD severe TMX). Total RNA was extracted from dissected mouse muscles homogenized in 10 volumes of TRIzol (ThermoFisher) using the TissueLyser LT (Qiagen), as per manufacturer's instructions followed by on-column DNaseI treatment and clean-up using the RNeasy mini kit (Qiagen). mRNAs were purified using poly(A) selection and then fragmented. First strand cDNA synthesis used random priming followed by second strand synthesis. The resulting double strand cDNA was end repaired, phosphorylated, and A-tailed. Adapters were ligated and PCR amplification was performed. The library was sequenced using the Illumina HiSeq2500 platform in a 1x50 base pair single-read configuration in Rapid Run mode, with a total of at least 120 million reads per lane.
attributes.tag: GEO Accession
attributes.value: GSM3473946