title: GSM3074699: Hesseltinella vesiculosa WGBS run1; Hesseltinella vesiculosa; Bisulfite-Seq
library_construction_protocol: Candida albicans, Candida auris, and Clavispora lusitianiae. MasturePure yeast DNA purification kit (Epicentre) following the manufacturer's instructions. Microbotryum lychnidis. Qiagen Kit 10243 (Courtaboeuf, France) following the manufacturer's instructions and using a Carver hydraulic press (reference 3968, Wabash, IN) for breaking cell walls. Lobosporangium transversale, Syncephalis fuscata, Spinellus fusiger, Parasitella parasitica, Kirkomyces cordense, Radiomyces spectabilis, and Sporobolomyces roseus. CTAB/Chloroform protocol (http://1000.fungalgenomes.org/home/protocols/high-quality-genomic-dna-extraction/). Aureobasidium pullulans, Coemansia reversa, Coemansia spiralis, Coprinopsis cinerea, Heterobasidion irregulare, Mixia osumundae, Phanerochaete chrysosporium, Pleurotus ostreatus, Pseudogymnoascus destructans, Tilletiopsis washingtonensis, and Wolfiporia cocos. A modified CTAB/Chloroform protocol (James et al. 2008; doi: 10.1111/j.1558-5646.2008.00462.x). Pholiota alnicola. EZNA Fungal DNA Mini Kit (Omega Bio-tek, Norcross, GA) following the manufacturer's instructions. MethylC-seq libraries were prepared using the protocol described in Urich et al. (2015; doi:10.1038/nprot.2014.114). Lambda phage DNA was used as a control for the sodium bisulfite non-conversion rate.
attributes.tag: GEO Accession
attributes.value: GSM3074699