title: GSM1680244: 1hUV-B-3; Chlamydomonas reinhardtii; RNA-Seq
library_construction_protocol: 2 mL of each culture was sampled following UV-B treatment and pellets of cells were frozen immediately. Total RNA was extracted with a NucleoSpin RNA II Total RNA isolation kit (Machery-Nagel). Purified RNA was treated using an RNase-Free DNase Set (Qiagen). DNase was heat inactivated in the presence of 2 mM EDTA. The libraries were prepared with 400 ng of total RNA as starting material and processed with the Illumina Truseq mRNA stranded kit according to the manufacturer instruction. The Accuprime High Fidelity PCR enzyme was used in the DNA fragment enrichment step.
attributes.tag: GEO Accession
attributes.value: GSM1680244