title: GSM4162753: Unmodified EVs rep1; Mus musculus; miRNA-Seq
library_construction_protocol: EV pellets (freeze-dried or not) were resuspended in Qiazol lysis buffer and RNA was extracted following RNeasy Micro kit with a DNase treatment step (Qiagen, Courtaboeuf, France). RNA was then quantified spectrophotometrically (Nanodrop ND-1000, Wilmington DE) and RNA quality was assessed by capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies Inc, Santa Clara, CA). Libraries were constructed using the NEXTFLEX Small RNA-seq kit v3 (Perkin Elmer, NOVA-5132-05) following the manufacturer's instructions. The resulting barcoded library was size selected on a Pippin HT (SAGE SCience) using 3 % agarose cassette (#HTG3004) and aiming for a size range between 126 base pairs (bp) and 169 bp.
attributes.tag: GEO Accession
attributes.value: GSM4162753