title: Islet YGO136 Illumina Paired RNAseq Sequence from NHGRI/NIDDK Tissue Biopsy Study
design: Illumina paired-end sequencing of mRNA from pancreatic islet, extracted and purified with Trizol, and sequencing library prepared using the Illumina TruSeq direction mRNA-seq protocol.  As described in Varshney, et al. PNAS 2016, up to 24 libraries are pooled, then sequenced on multiple lanes.  Each of the lane-library combinations is included in SRA as a separate run.  QC was performed on each run separately; all runs for a given experiment (library) are combined for analysis.