title: RNAseq of camptothecin-treated normal astrocytes
design: For library construction, 300 ng of high-quality RNA and the TruSeq Stranded Total RNA LT Sample Prep Kit (Illumina Inc) were applied. Firstly, the RiboZero technology (Illumina Inc.) was used to remove rRNA and preserve only poly(A) and other non-poly(A) transcripts, which were then fragmented. RNA fragments sized between 200500 bp were utilized to generate the sequencing libraries. Clustering was performed in an automated system cBot (Illumina Inc) and samples were sequenced with TruSeq SBS kit v5, single-read 72 cycles.