title: GSM1922702: E18.5_RNASeq; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq RNA from sorted E18.5 oocytes was extracted using the RecoverAll Total Nucleic Acid Isolation Kit (Ambion/ Thermo Scientific, AM1975) and purified using the RNA Clean & Concentrator-5 Kit (Zymo, R1013) with on-column DNase treatment (RNase-free DNase I, Thermo Scientific, AM2222). Ribosomal RNA was depleted from total RNA using the Ribo-Zero Magnetic Kit (Human/Mouse/Rat – Low Input, Epicentre, SCL24G). To generate RNA-seq libraries, RNA was reverse-transcribed using SuperScript III (Life Technologies, 18080044), with the second DNA strand synthesized using dUTPs and DNA polymerase I (NEB, M0210S). Libraries were constructed using the NEBNext DNA Library Prep Master Mix Set for Illumina (NEB, E6040S), with an additional step of dUTP excision by USER Enzyme (NEB, M5505S) before PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM1922702