title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of bovine genital ridges from male and female fetuses at three different time points corresponding to sex determination and early differentiation of the gonads
design: RNA-seq of bovine genital ridges from male and female fetuses at three different time points corresponding to sex determination and early differentiation of the gonads
library_construction_protocol: Genital ridges were dissected away from the mesonephros in sterile PBS and stored at -80 °C until all samples were collected. Three pairs of gonads from different animals were grouped to produce each sample. RNA was extracted using a Direct-zolTM RNA MiniPrep Kit (Zymo Research, CA, USA) following the manufacturer's protocol. DNA digestion was performed with RQ1 RNase-Free DNase (Promega, WI, USA) [60]. Total RNA was eluted in 50 µl RNase-free water. Immediately after extraction, total RNA was cooled to -80 ºC until further use.   RNA-seq libraries were prepared from three D35, three D39 and three D43 genital ridges from males and females
attributes.tag: Experimental Factor: age
attributes.value: 35
attributes.tag: Experimental Factor: sex
attributes.value: male