title: PA-seq of MEF_PD11
design: PD11 (population doubling times) MEF cells were obtained by isolating from mouse embryo and continously subculturing in DMEM with 10% FBS. Total RNA samples were isolated using TRIzol reagent (Life Technologies, NY, USA). The PA-seq libraries were constructed by following the protocol published before by Ni et al. (BMC Genomics,2013). The concentration of the RNA-seq libraries was measured by Qubit fluorometry (Life Technologies, NY, USA) before loading onto the Illumina HiSeq2000 platform.