title: Koala spleen (ISIS # 501090) transcriptome raw and processed reads (paired)
design: The spleen sample was collected from a koala euthanized due to senescence (ISIS # 502090), and then and immediately frozen.  The sample was shipped overnight on dry ice to Purdue University, where it was stored at -80 degrees Celsius.  RNA was extracted using TRIzol (Life Technologies) and Direct-zol columns (Zymo Research) via manufacturer's instructions.  Approximately 5 ug of RNA was used to create a cDNA library using random hexamer primers according to the TruSeq RNA sample preparation manual (Illumina).  The library was then sequenced on a lane of the Illumina HiSeq 2500 platform.  The "raw" reads are those resulting from the previous steps.  The "processed" were the result of further filtering.  Specifically, the adaptor sequences were removed, the reads were filtered for quality (PHRED quality score > 20 and length > 30 bp), and putative ribosomal RNA reads were removed using DeconSeq and a custom vertebrate rRNA database.