title: Bacterial 16S rRNA gene amplicons derived from soils exposure to TC for 7 days
design: Air-dried soil was filled into each polypropylene container for pot experiments. Brassica chinensis seeds were sown per pot. About two weeks after germination, Tetracycline were applied to the soil surface by watering. Bulk soils were sampled at day 7 after TC application. Soils were subjected to DNA extraction. The V3-V4 region of the 16S rDNA was selected for PCR amplification with the 338F/806R primer set. Primer 338F: 5'-ACTCCTACGGGAGGCAGC-3' Primer 806R: 5'-GGACTACHVGGGTWTCTAAT-3'