title: RNA-Seq of untreated healthy human peripheral blood CD4+ cells
design: CD4+ cells were isolated from fresh whole blood via positive magnetic bead selection (Miltenyi Biotec) and lysates were prepared using QIAzol (QIAGEN). Lysates were stored at -80ºC for 3 to 8 months and RNA was extracted using miRNAeasy (QIAGEN) immediately before cDNA library preparation and RNA-Seq.