title: GSM2816809: Early Prime 1; Mus musculus; RNA-Seq
library_construction_protocol: LCMV-Gp33-specific CD8 T cell transgenic (P14) cells were transferred into mice that had been infected 21 days earlier with LCMV-Cl13 (late prime) or into naïve mice immediately infected with LCMV Cl-13 (early prime). Sixty hours post-infection mice were sacrificed and splenocytes from 4-5 mice were pooled and single cell suspensions were stained for virus-specific P14 cells and FACSorted directly into RLT buffer. Naive P14 T cells were directly islated from P14 mice. RNA was isolated using a single cell RNA purification kit (Norgen Biotech Corp.) according to manufacturer's instructions. SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) was used per manufacturer's instructions for amplification of RNA and subsequent cDNA synthesis. All samples proceeded through NexteraXT DNA Library Preparation (Illumina) using NexteraXT Index Kit V1 or V2 Set A (Illumina) following manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2816809