title: GSM1254847: RLN-infected plant Inf1; Boehmeria nivea; RNA-Seq
library_construction_protocol: After seven days the ramie infected, the whole plant excluding root of four potted ramie were individually sampled. The sampled tissues were immediately frozen in liquid nitrogen and stored at -80° until use. The sample of tissue of CO1, CO2, Inf1 and Inf2 were individually extracted RNA by using TRIzol reagent (Transgene Company, Illkirch Graffenstaden Cedex, France) according to the manufacturer’s protocol. The RNA was stored at -80° for cDNA library preparation. RNA of four samples were separately used to construct the cDNA library with a fragment length range of 200 bp (±25 bp). Then, the paired-end sequencing was performed by using the Illumina sequencing platform (HiSeqTM 2000) according to the manufacturer’s instructions (Illumina, San Diego, CA).
attributes.tag: GEO Accession
attributes.value: GSM1254847