title: GSM4552086: Noninfected.Rep1.MeRIP; Mus musculus; RIP-Seq
library_construction_protocol: Spleens were removed, flash frozen on dry ice, and RNAs were harvested using Trizol reagent. Total RNA was purified using PolyATtract® mRNA Isolation System IV followed by RiboMinus transcriptome isolation kit for human/mouse. PolyA-selected and ribodepletion-treated mRNA samples were used for RNA fragmentation followed by immunoprecipitation with EpiMark® N6-Methyladenosine Enrichment Kit. Input RNAs and immunoprecipitated RNAs were used for library preparation using TruSeq Stranded mRNA kit. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4552086