title: GSM2125855: IgG; Homo sapiens; ChIP-Seq
library_construction_protocol: HeLa cells transiently expressing Flag-Zaa were cross-linked with 1% formaldehyde for 10 minutes at room temperature and cross-linking was quenched with 137 mM glycine. After nucleus extraction, isolated nucleus pellets were resuspended in Sonication buffer (50mM HEPES [pH7.9], 140mM NaCl, 1mM EDTA, 1% triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, 1X protease inhibitor). The chromatin was sonicated to the range of 100-300 bp, and then immunoprecipitated overnight using an anti-Flag antibody (F1804, Sigma, USA) or anti-mouse IgG antibody (sc-2025. Santa cruz, USA). Input was taken from starting material before immunoprecipitation. ChIP DNA for Zaa biological replicates and control DNA (IgG ChIP and input) were used for sequencing library construction. Sequencing libraries were prepared using Aceel-NGS DNA library kits according to the manufacturer's instructions.  PCR products were purified with HiAccuBead (AccuGene, Korea). Purified PCR products are eluted in low-EDTA TE buffer.
attributes.tag: GEO Accession
attributes.value: GSM2125855