title: GSM4668320: SC-RNASeq_YC1_S2_L001; Mus musculus; RNA-Seq
library_construction_protocol: Mandibles were carefully dissected under a stereomicroscope. The surrounding jaw bones were removed by scalpel and the proximal region was then severed. Dental pulp with dental epithelium was isolated, cut into pieces, collected in cold PBS, and digested with 2mg/ml collagenase type I. Single Cell 3ʹ Gene Expression libraries comprise standard Illumina paired-end  constructs which begin with P5 and end with P7. 16 bp 10x Barcodes are encoded at  the start of TruSeq Read 1, while 8 bp sample index sequences are incorporated as  the i7 index read. TruSeq Read 1 and Read 2 are standard Illumina sequencing primer  sites used in paired-end sequencing. TruSeq Read 1 is used to sequence 16 bp 10x  Barcodes and 12 bp UMI. Sequencing these libraries produce a standard Illumina BCL  data output folder.
attributes.tag: GEO Accession
attributes.value: GSM4668320