title: RNAP2 ChIPseq in NEG control treated HEK293T/17 cells
design: HEK293T/17 cells grown in DMEM supplemented with 10% FBS, Glutamax™ (invitrogen), and penacillin/ streptamycin Cells were transfected with control or siRNA targeting FUS using RNAiMAX™ lipid (invitrogen) using a reverse transfection strategy mRNA was extracted by Trizol™ (invitrogen) and genomic DNA was extracted from sonicated and clarified cell lysates using antibodies mRNA and DNA libraries were prepared using Truseq™ RNA and ChIPseq illumina kits