title: GSM4056958: sRNAs from Huh7.5.1 cells; Homo sapiens; miRNA-Seq
library_construction_protocol: RNA was extracted from Huh7.5.1 cells using Tri-reagent (Ambion) as per manufacturer's instructions. Small RNA libraries were prepared from 25 μg of total RNA as previously described (Pfeffer, S. 2007. Identification of virally encoded microRNAs. Methods Enzymol. 427:51-63), except that PCR products were not concatemerized but directly sent for large-scale sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4056958