title: GSM2652140: Lphn2-siRNA-2 Rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA for cell pool Nanostring analysis and RNA sequencing was isolated with the RNeasy Micro Kit (Qiagen) according to the corresponding manufacturer`s instructions. For RNA-seq, DNA was digested on-column with DNaseI digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). 2µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry
attributes.tag: GEO Accession
attributes.value: GSM2652140