title: NextSeq 500 paired end sequencing; RNA-seq of HER2-positive breast cancer BT474 cell line in response to KDM5 ihibition
design: RNA-seq of HER2-positive breast cancer BT474 cell line in response to KDM5 ihibition
library_construction_protocol: BT-474 cell line were grown in DMEMF12, 5% FBS, 24h after seeding, cells were  treated with vehicle or KDM5-inh1 (1 μM) for 48 hours. BT-474 cell line were grown in DMEMF12, 5% FBS.  24h after seeding, cells were  treated with vehicle or KDM5-inh1 (1 μM) for 48 hours. RNA was extracted with the RNeasy Kit  (QUIAGEN), according to the manufacturer instruction. RNA libraries were prepared for sequencing using TruSeq RNA Library Prep Kit v2 (Stranded) from Illimuna®
attributes.tag: Experimental Factor: compound
attributes.value: KDM5-inh1
attributes.tag: Experimental Factor: dose
attributes.value: 1