title: RNA-seq of NIH3T3 over-expressing CAMTA1 (replicate 1)
design: "Total RNA was extracted using Trizol Reagent (Invitrogen-Life Technologies). RNA samples were column purified using the RNeasy Mini Kit (Qiagen, Valencia, CA USA) followed by DNase treatment (Invitrogen-Life Technologies). RNA quality was assessed using the Agilent Bio-analyzer (Agilent Technologies, Santa Clara, CA USA). RNA-sequencing libraries were generated using Illumina TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and quantitated using the Agilent Bioanalyzer."