title: GSM4669618: miR_only_scRNA-seq_D20; Homo sapiens; RNA-Seq
library_construction_protocol: Reprogramming times (Day 0, 5, 10, 15, and 20) were stacked so that cells at different time points of neuronal conversion could be collected on the same day. Cells were washed with sterile PBS, detached using Trypsin, and collected by centrifugation in 10% FBS in DMEM. Cells were washed using BSA in PBS by gentle pipetting. Collection and wash steps were repeated once, then cells were counted on a hemocytometer and volume was adjusted to achieve 1,000 cells/µL with 10% FBS in DMEM. Cells were placed on ice to be immediately proceeded with 10X Genomics Single Cell processing. For single-cell library preparation, we used the 10x Genomics platform with the Chromium Single Cell 3′ Reagents Kits according to manufacturer instructions. cDNA libraries were quantified on an Agilent BioAnalyzer. These libraries were sequenced on a NovaSeq S4.
attributes.tag: GEO Accession
attributes.value: GSM4669618