title: RNA-Seq of Oryzias javanicus: adult female kidney
design: Total RNA was extracted using Quiagen RNeasy kit. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA HT sample prep kit. Briefly, mRNA were selected using poly-T beads. Then, RNA were fragmented to generate double stranded cDNA and adaptators were ligated to be sequenced. 10 cycles of PCR were applied to amplify libraries. Library quality was assessed using a Fragment Analyzer and libraries were quantified by QPCR using the Kapa Library Quantification Kit. RNA-seq experiments have been performed using a paired-end read length of 2x150 pb with the Illumina TruSeq SBS HiSeq3000 sequencing kits.