title: GSM3602399: fry1-6_total_1; Arabidopsis thaliana; miRNA-Seq
library_construction_protocol: Total RNA extraction: 12 day seedlings of Col, fry1-6 and fry1-6 double mutants  were ground in liquid nitrogen, RNAs were extracted by Tri Reagent (Molecular Research Center) following the manufacturer's instructions. 20ug total RNA was resolved on 15% Ura PAGE, and15-40nt small RNAs were recovered by gel purification. The recovery small RNA products  were used for small RNA  library construction with NEBNext® Multiplex Small RNA Library kit (NEB, E7300S).
attributes.tag: GEO Accession
attributes.value: GSM3602399