title: GSM2616849: 3--24; Homo sapiens; miRNA-Seq
library_construction_protocol: 8 h and 24 h after infection, total RNA was extracted from the DENV-infected cells and the corresponding mock-infected cells using TRIzol reagent (Invitrogen China Ltd., Shanghai, China) according to manufacturer's instructions.  Subsequently, the isolated RNA was treated with DNase I (TaKaRa, Dalian, China) to remove DNA contaminants. Total RNA was electrophoresed by 15% denaturing polyacrylamide gel electrophoresis (PAGE) with 8 M urea, and small RNAs, of 15～30 nt, were excised out of the gel and purified. Subsequently, 5' RNA adaptors and 3' RNA adapters were ligated to the sRNA ends. The sRNAs with adapters were amplified by RT-PCR using the Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions, and the generated cDNA libraries were sequenced using Illumina HiSeqTM 2500 sequencing technology(Shanghai biotechnology corporatio Ltd, Shanghai,China).
attributes.tag: GEO Accession
attributes.value: GSM2616849