title: GSM3307417: Ago2_miR106b_4; Mus musculus; RNA-Seq
library_construction_protocol: Polyadenylated RNA was isolated using oligo(dT) binding, followed by chemical fragmentation and preparation of non-strand-specific, single-end RNA-Seq libraries, as per Methods Mol Biol. 2015;1299:27-49, PMID 25836573. RISC RNA was isolated from immunoprecipitated Ago2 via direct incubation of immunoprecipitates with Trizol reagent (Invitrogen).  Chemical fragmentation and preparation of single-end RNA-Seq libraries proceeded as for polyadenylated RNA. non-strand-specific, single-end RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM3307417