title: Mouse 3a colon tissue expression
design: Eight cDNA libraries (FF n=4 and MF n=4) were constructed with the "mRNA-seq Sample Prep" kit from Illumina following the manufacturer's protocol (Illumina, Hayward, CA). Each sample was individually barcoded using Illumina's "Multiplexing Sample Prep" kit by adding unique six-base sequence indexes into the adapter. Samples were mixed into two pools (pool 1: 10a, 3a, 6a, 4a and pool 2: 5B, 3C, 1C, 2B), and each pool was sequenced on one lane on a Genome Analyzer IIx using chemistry version 4 for a total of 101 cycles.