title: GSM4552076: BJ APH r4 Late S; Homo sapiens; OTHER
library_construction_protocol: Genome-wide replication timing profiles were constructed as previously described (Marchal et al., 2018). Briefly, cells were pulse labeled with BrdU and separated into early and late S-phase fractions by flow cytometry.  From the total gemnomic DNA extracted from each fraction, BrdU-labeled DNA was enriched using anti-BrdU immunoprecipitation. Sequencing libraries of BrdU-labeled DNA from early and late fractions were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370).
attributes.tag: GEO Accession
attributes.value: GSM4552076