title: Illumina HiSeq 2000 sequencing; Transcriptome before and 10 min after valine induced isoleucine starvation in Escherichia coli K12 grown in minimal MOPS glucose medium at 37° C
design: Transcriptome before and 10 min after valine induced isoleucine starvation in Escherichia coli K12 grown in minimal MOPS glucose medium at 37° C
library_construction_protocol: Cells harvested right before and 10 min after valine addition. 280 ml of cultures were harvested, centrifuged and washed in 0° C biotin-free medium 10 generations of growth on MOPS minimal medium supplemented with 0.2% glucose and 5 µg/ml biotin Following collection, resuspension in 2 ml of lysis buffer, followed by sonication. Phenol extraction of aliquote followed by storage at -80°C libraries constructed WITHOUT rRNA depletion by 1) fragmentation of RNA 2) first strand synthesis with random primers 3) second strand synthesis with random primers 4) end repair and A tailing 5) adapter ligation 6) size selection and quantification and 7) sequencing
attributes.tag: Experimental Factor: none
attributes.value: compound