title: GSM2896742: 451Lu_bulk_resistant_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from flash frozen cell pellets containing ~10e6 cells, using the Trizol Plus RNA Purification Kit which combines standard TRIzol reagent with the Ambion PureLink RNA Mini kit. RNA extracts were then checked to ensure RIN numbers ~9-10. Stranded RNA-seq libraries were constructed using 500ng of input total RNA per sample and the Illumina TruSeq Stranded Total RNA Sample Prep LS kit, following the Illumina protocol as prescribed, including the use of RiboZero Gold for magnetic bead based rRNA depletion. RNA-Seq Libraries were sequenced in pools of 3 barcoded samples per lane on an Illumina HiSeq using the PE101 format to a depth of ~50 million fragments per sample.
attributes.tag: GEO Accession
attributes.value: GSM2896742