title: RNA-Seq of human fetal retina
design: RNA was treated with RQ1 DNase (Promega, Madison, WI) for an hour and then purified using miRNeasy kit (Qiagen, Germantown, MD). 5  g of each sample was then processed using Ribozero (ScriptSeq Complete kit; Epicenter, Illumina) to deplete ribosomal RNA.