title: Thoroughbred 2
design: Input genomic DNA (1–5µg) is fragmented by hydrodynamic shearing to generate <800 bp fragments. The fragments are blunt ended and phosphorylated, and a single A nucleotide is added to the 3 ends of the   fragments in preparation for ligation to an adapter that has a single base T overhang.  Adapter ligation at both ends of the genomic DNA fragment confers different sequences at   the 5 and 3 ends of each strand in the genomic fragment. The products of this ligation   reaction are purified and size selected by agarose gel electrophoresis. Size selected DNA is   PCR amplified to enrich for fragments that have adapters on both ends. The resulting sample library is again purified and size selected by agarose gel electrophoresis. The final purified product is then quantitated prior to seeding clusters on a flow cell.