title: GSM3307257: B3: Cold treatment 1 hour rep3; Glycine max; RNA-Seq
library_construction_protocol: All harvested samples were immediately frozen in liquid nitrogen and stored in -80 °C until RNA isolation. RNA from 100 mg of leaf samples pulverized in liquid nitrogen was obtained with the RNeasy Plant Mini kit (Qiagen Cat. No. 74903, http://www1.qiagen.com/). The lysis buffer (RLT) containing GITC with 157 mM β-mercaptoethanol was used for RNA isolation. RNA was treated with DNase (Qiagen Cat. No. 79254) during isolation, was then eluted from column with water, and quantified by absorbance at 260nm. Illumina RNA TruSeq v2 sample preparation protocol
attributes.tag: GEO Accession
attributes.value: GSM3307257