title: ChiP-seq of Homo sapiens cell line TC32
design: Libraries for Input and IP samples were prepared by the Van Andel Genomics Core from 10 ng of input material and either 10ng or all available IP material using the KAPA Hyper Prep Kit (v5.16) (Kapa Biosystems, Wilmington, MA USA). Prior to PCR amplification, end repaired and A-tailed DNA fragments were ligated to Bioo Scientific NEXTflex Adapters (Bioo Scientific, Austin, TX, USA).  Quality and quantity of the finished libraries were assessed using a combination of Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.), QuantiFluor dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems). 50 bp, paired end sequencing was performed on an Illumina NovaSeq sequencer using a 100 bp S1 sequencing kit (Illumina Inc., San Diego, CA, USA).  Base calling was done by Illumina RTA v3.0 software and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq2 v2.20.0.