title: ETMR2-smRNA
design: Samples were homogenized in nitrogen using mortal and pestle. Brain powder was immersed in TRIzol (Invitrogen) and subjected to a second round of homogenization using a POLYTRON for 30 seconds. Total RNA was extracted using miRNeasy kit (including a DNaseI treatment step) and quality assessed by Agilent 2100 BioAnalyzer (Agilent Technologies). Quality of the libraries was assessed using Agilent 2100 BioAnalyzer (Agilent Technologies). Samples were indexed (10 per line) and sequenced in an Illumina HiSeq 2000.