title: GSM2561624: MGAS2221 + vector; Streptococcus pyogenes; RNA-Seq
library_construction_protocol: Total RNA was isolated and rRNAs were depleted using the Ribo-Zero Gram-Positive rRNA Removal Kit (Epicentre). The rRNA depleted RNA was used to generate cDNA libraries using the ScriptSeq kit (Epicentre).  Briefly, RNA was fragmented, cDNA was synthesized using random hexamers containing a 5′ tagging sequence, the RNA was hydrolyzed, and the cDNA was tagged at the 3′ end.  A limited number of PCR cycles (n=12) were used to amplify the libraries via the 5′ and 3′ tags (the libraries were barcoded by using different primers), and the libraries size-selected (170 to 300 bp).  The size-selected and barcoded libraries were run on an Illumina MiSeq flowcell.
attributes.tag: GEO Accession
attributes.value: GSM2561624