title: GSM1258239: E14_Cxxc1_GFP; Mus musculus; ChIP-Seq
library_construction_protocol: Experiments were performed with 3.3 million cells and 3 microgram antibody per ChIP. Crosslinking was performed on the culture plates for 20 min and ChIP’ed DNA was purified by Qiaquick PCR purification Kit (QIAGEN). DNA samples from ChIP-Seq were prepared for sequencing by end repair of 20 ng DNA as measured by Qubit (Invitrogen). Adaptors were ligated to DNA fragments, followed by size selection (~300 bp) and 14 cycles of PCR amplification. Integrity of DNA libraries was confirmed by running the products on a Bioanalyzer (BioRad). Cluster generation and sequencing (36 bp) was performed with the Illumina Genome Analyzer IIx (GAIIx) sequencing platform according to standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM1258239