title: ChIPSeq of Flag-Anp32e in HeLa Cells transfected with Flag-Anp32e
design: HeLa cells stably expressing Flag-Anp32e were used for ChIP-seq experiments, cells were crosslinked with 1% (v/v) formaldehyde for 10 min at room temperature and the reaction was quenched by adding glycine to 0.15 M. Cells were washed with PBS and then resuspended in 20 mM Tris, pH 8.0, 300 mM NaCl and 0.1% SDS and sonicated to 200- to 500-bp chromatin fragments using Bioruptor (Diagenode). ChIP experiments were performed as previously reported(Zhu et al., 2005). Sequencing libraries were generated according to Illumina recommendations by using NEBNext* DNA Library Prep Master Mix Set for Illumina*. The libraries were single-end sequenced with HiSeq 2000.