title: GSM2897333: 451Lu_10x_singlecell_parental; Homo sapiens; RNA-Seq
library_construction_protocol: A 10X Genomics Chromium machine was used to capture 3,000-4,000 single cells and cDNA was prepared using the Single Cell 3' Protocol recommended by the manufacturer. A Chromium Single Cell 3' Library and Gel Bead Kit V2 (PN-120237), Chromium Single Cell 3' Chip Kit V2 (PN-120236) and Chromium i7 Multiplex Kit (PN-120262) were used with a 10x Genomics Chromium for Single-Cell Library Preparation Instrument, per the manufacturer's specifications and manuals and then sequenced paired-end 150 bp on HiSeq 4000 to a depth of 90000 UMI per cell.
attributes.tag: GEO Accession
attributes.value: GSM2897333