title: GSM1258038: Dpse_ovary_rep1; Drosophila pseudoobscura; RNA-Seq
library_construction_protocol: Virgin adult males and females were collected and aged 6-10 days before dissecting out testes or ovaries. All attached ducts were removed from the gonad tissue. Tissues were stored in RNAlater (Ambion) at -20C until RNA extraction. Immediately before RNA extraction RNAlater was removed by adding 1 mL RNase-free phosphate-buffered saline (PBS), centrifuging the sample 5,000 x g at 4 degrees C for 5 minutes, replacing the supernatant with 1 mL fresh PBS, centrifuging again, and replacing the supernatant with extraction buffer (XB) from the Arturus(R) PicoPure (R) kit according to the manufacturer's instructions. We included an on-column DNase digestion in the RNA extraction protocol. Libraries were constructed following the Illumina TruSeq RNA Library Construction Kit. Poly-A+ RNA was selected, reverse-transcribed using random (hexamer) priming, and the resulting cDNA fragments sheared to ~120-200 bp fragments. These fragments were used for adapter ligation and sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1258038