title: GSM2995367: P11-Ca-1-RNASeq; Homo sapiens; ssRNA-seq
library_construction_protocol: RNA was extracted from O.C.T embeded frozen colon biopsies (P1-SSP-3, P2-SSP-4, P5-SSP-7, P6-SSP-8, P8-TSA-2, P9-FAP-2, P10-FAP-3, P11-Ca-1, P12-Ca-2, P1-GU-1, P13-NA-1, P13-NS-1, P14-NS-2, P15-NA-3) as explained above. Total RNA was DNase treated using TURBO DNA-free Kit (Ambion), and its quality was assessed on Bioanalyzer RNA 6000 Nano Chip. For ribosomal RNA depletion, the total RNA was treated with RiboMinus kit (Life Technologies). Directional RNAseq library was performed according to Illumina's protocol. Libraries were sequenced using 50bp single-end format on Illumina HiSeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM2995367