title: GSM3307958: Total SEnd-seq rents mutation log phase rep2; Escherichia coli; OTHER
library_construction_protocol: E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer (5% phenol in ethanol) immediately to the culture medium before harvest and stay on the ice for 15 minutes. Cell pellets were collected by centrifugation (6,000 rpm, 5 minutes at 4 °C), thoroughly resuspended in 100 μl of lysozyme solution [2 mg/ml in TE buffer (10 mM Tris-HCl and 1 mM EDTA)], and incubated for 2 minutes. The cells were immediately lysed by adding 1 ml of TRIzol Reagent (Invitrogen, 15596) and the RNA was extracted following manufacture's instruction. A new RNA-seq method SEnd-seq was used to prepare the SEnd-seq library, and  standard RNA-seq was prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101) following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3307958