title: GSM4119064: KPC2156_pancreatic cell line; Mus musculus; RNA-Seq
library_construction_protocol: RNA was collected from cell lines or snap-frozen tumors using TRIzol reagent according to the manufacturer's instructions Libraries were prepared by the Van Andel Genomics Core from 500g of total RNA using the KAPA Stranded mRNA-Seq Kit (v4.16) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 nts. Prior to PCR amplification, cDNA fragments were ligated to Bio Scientific NEXTflex Adapters (Bio Scientific, Austin, TX, USA). Quality and quantity of the finished libraries were assessed using a combination of Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.), QuantiFluor® dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems). Individually indexed libraries were pooled and 75 bp, single-end sequencing was performed on an Illumina NextSeq 500 sequencer using a 75 bp HO sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) to a minimum read depth of 30M reads per library. Quality and quantity of the finished libraries were assessed using a combination of Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.), QuantiFluor® dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems). Individually indexed libraries were pooled and 75 bp, single-end sequencing was performed on an Illumina NextSeq 500 sequencer using a 75 bp HO sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) to a minimum read depth of 30M reads per library.
attributes.tag: GEO Accession
attributes.value: GSM4119064