title: RNA-seq of Solanum lycopersicum: Heat schock seed
design: Total RNA was extracted from 100 seeds using the NucleoSpin RNA plant Kit (Macherey-Nagel). The quality and quantification of the total RNA samples were evaluated in 2100 Bioanalyzer and samples with RIN higher than 7.2 were used. The RNA-Seq protocol included the fragmentation and purification of the messenger RNA. The next step was the amplification for the construction of cDNA libraries: hybridization and binding of adapters, reverse transcription, cDNA purification, and finally, amplification and quantification of the amplified cDNA. Constructed libraries were 100 bp paired-end sequenced.