title: GSM2995107: pDC_stim_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: We sorted pure populations of pDCs and cDCs based on the following panel of standard antibodies (from BioLegend and BD Pharmingen): CD3 (HIT3a), CD19 (HIB19), CD56 (HCD56), CD14 (HCD14), HLA-DR (LN3), CD11c (Bu15) and CD123 (7G3). pDCs were defined as: CD3- CD19- CD56- CD14- HLA-DR+ CD11c- CD123+. cDCs were defined as: CD3- CD19- CD56- CD14- HLA-DR+ CD11c+. Following sorting, we extracted RNA using TRIzol (Invitrogen) We performed bulk RNA-seq using a modified version of the SMART-Seq2 protocol followed by library preparation using the Nextera XT DNA sample prep kit (Illumina) as in Mayer et. al., 2018.
attributes.tag: GEO Accession
attributes.value: GSM2995107