title: GSM1690099: S2_Mate pair; Drosophila melanogaster; OTHER
library_construction_protocol: High molecular weight genomic DNAs from S2, Kc167, BG3 and OSC cells were isolated by standard method (Sambrook and Russell 2001) with modifications. Briefly, cells were incubated in lysis buffer (50 mM Tris-HCl at pH 8.0, 25 mM EDTA, 0.2% Triton X-100, 1% sarcosyl) with 20 mg/mL proteinase K for 16 h at 37⁰C. DNA was sequentially extracted with phenol, phenol-chloroform, chloroform and then precipitated with 2.5 volumes of ethanol, washed 3 times with 70% ethanol, dried and dissolved in TE. The fragmentation of genomic DNAs into 8-10 kb fragments was done by “Evrogen” (http://www.evrogen.com/) with the use of Nextera Mate Pair library Sample Prep kit (Illumina), followed by the TruSeq DNA Sample Prep kit. The libraries were quantitated by qPCR.
attributes.tag: GEO Accession
attributes.value: GSM1690099