title: NextSeq 500 paired end sequencing; RNAseq of proneural glioblastoma stem-like cells 72 hours after infection with human cytomegalovirus (CMV) Towne strain
design: RNAseq of proneural glioblastoma stem-like cells 72 hours after infection with human cytomegalovirus (CMV) Towne strain
library_construction_protocol: Samples were cells in culture, centrifuged and pellets collected RNA was extracted from cell pellets using Trizol as described by the manufacturer. RNA degradation and contamination were monitored on 1% agarose gels and RNA integrity was assessed with the RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system  The libraries were prepared from 500ng of total RNA using Illumina TruSeq strand specific mRNAseq library prep kit according to manufacturer's protocol.
attributes.tag: Experimental Factor: infect
attributes.value: none