design: High throughput transcription profiling of mature green fruit from S.                 lycopersicum plants. Transcriptomic reads were mapped to the S. lycopersicum genome                 sequence enabling the identification of polymorphisms and quantification of the                 allele specific gene expression.
library_construction_protocol: Total RNA was extracted from mature green fruits of                     S. lycopersicum plants. PolyA RNA was isolated by homogenization of frozen                     ground tissue in RNA extraction buffer (8M guanidinium hydrochloride; 20mM MES;                     pH7) and phenol:chloroform:isoamyl alcohol (v/v 50:48:2). After centrifugation                     the aqueous phase was re-extracted with phenol:chloroform: isoamyl alcohol and                     then subjected to three successive precipitations with ethanol/sodium acetate;                     4M LiCl; and ethanol/sodium acetate. The final pellet was washed in 70% ethanol                     and re-suspended in diethylpyrocarbonate treated water. Highly purified poly(A)+                     RNA was prepared using Magnetight Oligo(dT) Particles, Straight A’s TM mRNA                     Isolation System (Novagen, Inc.) following the manufacturer’s instructions. .                     Whole transcriptome libraries were prepared from each sample, following the                     procedures of the SOLiD Whole Transcriptome Analysis Kit (Life Technologies).                     Libraries were barcoded and pooled prior to ePCR amplification. One flowcell was                     loaded and 50bp fragments were sequenced with a SOLiD version 3 instrument (Life                     Technologies, Carlsbad, CA, USA).