title: Identification of the conserved and novel miRNAs in mulberry by high-throughput sequencing
design: Small RNA libraries of M. notabilis were constructed as described elsewhere with a few modifications (Wang et al. 2012). Briefly, the total RNA of three M. notabilis tissues was extracted using RNAiso plus in accordance with the manufacturers’ instruction (D9108A, Takara, China).  Samples were then subjected to 15% denaturing polyacrylamide gel electrophoresis (PAGE). Small RNA fragments of 18-30 nt were separated, purified, and ligated with the 5’, 3’adapter sequentially. After reverse transcription and PCR, about 20 μg products of three tissues were sequenced separately using Illumina HiSeq-2000 (BGI-Shenzhen, China)