title: M5
design: Strand-specific RNA-seq library construction was performed using own developed protocol compatible with the TrueSeq Stranded Total RNA Library preparation kit (Illumina ), based on Zhong, Joung, Zheng, Chen, Liu, Shao, Xiang, Fei and Giovannoni (2011). All cDNA libraries obtained from each RNA sample treatment were size selected by AMPure XP Beads and then PCR amplified using Illumina primers. The library quality determination was done using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA).