title: 16S rRNA bacterial composition, Obregon pond 1 d80
design: Sample collected from intestine of 5 whiteleg shrimp raised in outdoor pond, Obregon pond 1. Shrimp d 80 of age. Sample kept in ethanol 100% for transportation, then stored at -20C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using repeated beating followed by Qiagen stool kit. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes using 27F-519R primers. Sequencing using Illumina MiSeq2X300.