title: DNA_reactor-N1-biological replicate 2_Day22
design: DNA was extracted It was used for amplification of the  hypervariable region V4V5 of the 16S RNA with the primers 515F(50-GTGYCAGCMGCCGCGGTA-30) and 928R(50-CCCCGYCAATTCMTTTRAGT-30) . Sequencing was performed using IoN216 chip and the Ion PGM Sequencing 400 Kit.