title: GSM1608511: GM12878_HIC_7; Homo sapiens; OTHER
library_construction_protocol: 25 million cells for GM12878 were cross linked and chromatin digested with HindIII. DNA overhangs were biotinylated and proximity ligated under dilute conditions to favor ligation of fragments in three-dimensional proximity. DNA was then sheared, biotinylated fragments were enriched with streptavidin beads and prepared for Illumina sequencing For ChIP-Seq, nuclear lysates were sonicated using a Branson 250 Sonifier (power setting 2, 100% duty cycle for 7 x 30-s intervals). Clarified lysates corresponding to 20 million cells were treated with 1-5ug of antibody coupled to Protein G Dynabeads (Invitrogen #10003D, New York). The protein-DNA complexes were washed with RIPA buffer and eluted in 1% SDS TE at 65°C. ChIP DNA sequencing libraries were generated according to Illumina DNA Tru-Seq DNA Sample Preparation Kit Instructions (Illumina Part # FC-121-2001, San Diego, CA).
attributes.tag: GEO Accession
attributes.value: GSM1608511