title: Control DIV 18.1
design: R1 embryonic stem cells (ESCs) were maintained and differentiated into highly enriched glutamatergic neurons as described in Hubbard, et al (BMC Neuroscience 2012, 13:127).   High quality RNA was isolated with the RNeasy mini kit protocol.  Reverse-transcribed RNA was prepared for  high throughput sequencing using the TruSeq RNA Prep Kit (Illumina, San Diego, CA).  Paired-end sequence data was generated over 2x50 cycles using an Illumina HiSeq 2000.