title: GSM4550971: Sample_24_RA0401-48h-2; Homo sapiens; RNA-Seq
library_construction_protocol: Human FLS were isolated from synovial tissue by arthroplasty or synovectomy from OA or RA joints. Cells were treated with NETs for 48 hours and RNA was isolated using Direct-zol RNA Miniprep kit. cDNA libraries were prepared with poly(A) tail enrichment using NEBNext Ultra II RNA Library Prep Kit following manufacturer instructions (New England Biolabs Ipswich, MA). RNA-Seq libraries were sequenced on an Illumina Hiseq 3000 as single end with 50 base reads. 
attributes.tag: GEO Accession
attributes.value: GSM4550971