title: GSM2653139: NPAI_c2_H3K27me3; Mus musculus; ChIP-Seq
library_construction_protocol: Tumor NS were collected and suspended in digestion buffer.  Digestion was performed using microccocal nuclease to obtain 150 pb DNA fragments. Histone-DNA complexes were isolated with the desired H3 me3 specific antibody. Libraries for ChIP-seq were prepared using the TruSeq ChIP Sample Preparation Kit. Input and IP DNA  was blunt-ended and phosphorylated, followed by the addition of a single adenine nucleotide prior to ligation with an adapter duplex with a T overhang. Ligated products were size selected by agarose gel electrophoresis, purified and PCR amplified for the final library preparation.
attributes.tag: GEO Accession
attributes.value: GSM2653139