title: GSM4217568: ChIP-exo_0hr_Pol2; Mus musculus; ChIP-Seq
library_construction_protocol: Cells are lysed, the nuclear fraction retained, and the chromatin is fragmented by sonication to 100-500bp. In vivo protein-DNA interactions are preserved through formaldehyde crosslinking. Cells are lysed, the nuclear fraction retained, and the chromatin is fragmented by sonication to 100-500bp. DNA fragments interacting with the protein of interest are enriched by ChIP, the important lambda exonuclease digestion effectively footprinting the left and right 5' DNA borders of the protein-DNA crosslink site, and a library is prepared by adding sequencing adapters according to manufacturer's instructions. ChIP-exo enriched DNA fragments are sequenced from the left and right 5' DNA borders of the protein-DNA crosslink site.
attributes.tag: GEO Accession
attributes.value: GSM4217568