title: RNA-seq of G.platifrons:insitu gill
design: Gill tissue from mussels in InSitu, UnP, UnP2 and P2M group were subsequently subjected to RNA extraction using Trizol reagent. The integrity and purity of total RNA extracted were checked by agarose gels and Bioanalyzer 2100 (Agilent). The concentration of total RNA was then quantified using NanoPhotometer spectrophotometer (Implen) and Qubit Fluorometer (ThermoFisher). RNA samples from individual mussel were subsequently subjected to cDNA library construction after removing rRNA by Ribo-ZeroTM Magnetic Kit (Epicentre). The enriched RNA was then fragmented and reversely transcribed into cDNA with random primers. After ligation with adapters and purification of PCR amplicons, the cDNA libraries were then sequenced using Illumina HiSeqTM 2500 platform with pair-end reads. The resulting sequencing data were then uploaded and deposited at The National Center for Biotechnology Information