title: GSM1258236: IgG-ChIPSeq; Mus musculus; ChIP-Seq
library_construction_protocol: CD8 T cells were then crosslinked, sonicated with Covaris instrument, and ChIP was done with either Tcf1 antibody or control IgG.  For control IgG, pre-immune rabbit serum that we obtained from a different antibody production project was used to match the anti-Tcf1 (which is unfractionated rabbit antiserum rather than IgG).The immunoprecipitated fragments were reverse-crosslinked and purified. chromatin DNA fragments were end-repaired, and ligated with Illumina adaptors. The DNA was then PCR amplified with for 18 cycles and library fragments of 220-400 bp were purified from gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM1258236