title: GSM1264649: DGE of Kasa-1; Oryza sativa; OTHER
library_construction_protocol: Leaf samples taken from two-month-old plant were harvested for RNA extraction using TRIzol (Invitrogen) as described by the manufacturer. Total RNA was extracted from Y103-1 and Kasa-1 with TRIZOL® Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The RNA samples were incubated for 30 min at 37°C with 10 units of DNaseI (Takara, Dalian, China) to remove residual genomic DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at 260 nm/280 nm (A260/A280) using a Nanodrop® ND-1000 spectrophotometer (LabTech, Holliston, MA, USA). The samples had an average RIN value of 8.9 according to Labon-chip analysis using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM1264649