title: GSM4550989: OEC-M1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from immortalized oral keratinocyates and oral cancer cells using Trizol reagent (Invitrogen), and further purified on RNeasy columns (QIAGEN) according to the manufacturer's instructions. The quality of RNA was assessed with the Agilent Bioanalyzer 2100 using RNA Nano reagents (Agilent) to assure RIN>8. Two rounds of polyA selection were performed using SeraMag oligo dT magnetic beads (SeraDyn) following the Illumina protocol. Transcriptome libraries from the mRNA fractions were generated following the RNA-SEQ protocol (Illumina) and size selected using 3% NuSieve agarose gels (Lonza) followed by gel extraction using QIAEX II reagents (QIAGEN) with a gel melting temperature of 32oC. Libraries were quantified using the Bioanalyzer 2100 using the DNA 1000 protocol and reagents (Agilent). Each sample was sequenced in a single lane with the Illumina Genome Analyzer II (80 nucleotide read length). stranded mRNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM4550989