title: RNA-seq of M.rosenbergii: adult male HPT
design: The cDNA libraries were normalized using a duplex-specific nuclease and constructed by PCR amplification using random hexamer primed cDNAs. Finally, the samples were sequenced using paired-end strategy and an Illumina MiSeq instrument (Illumina Inc.). Raw reads from sequencing were then filtered in order to remove those reads that contain adaptor, unknown nucleotides (>5%), or low quality reads (>20% of low quality bases). De novo assemblies were performed individually and combined by Trinity software using trimmed reads