title: PTIP52_L2_382
design: The genomic DNA was isolated from either freshly dissected lung tumours or lobes using the QIAamp DNA mini kit (Qiagen). For each target gene or potential off-target sites, a genomic region containing the target sequence was PCR-amplified using specific primers and gel purified, followed by library construction and high-throughput sequencing analysis (Illumina Hiseq 2500). 