title: GSM2897304: 013 (4164-1001_p.10); Homo sapiens; RNA-Seq
library_construction_protocol: AllPrep DNA/RMA/miRNA universal kit (Qiagen). RNA sequencing and data analysis were outsourced to Arraystar (USA). In brief, 1-2 µg of total RNA were enriched by oligo(dT) magnetic beads (rRNA removal), and sequencing libraries were prepared using the KAPA Stranded RNA-Seq library prep kit (Illumina) which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina HiSeq 4000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina HiSeq instrument.
attributes.tag: GEO Accession
attributes.value: GSM2897304