title: ITS amplicons of spore bank ectomycorrizal fungal root tips: Rim Fire pre-fire soils from plot CA2 with Pinus muricata host: Sample CA2_0_prefire
design: "Ectomycorrhizal root tips were harvested under dissecting scope from pine seedlings grown in each sampling location.  Root tips from pine seedlings grown in the same sampling location with the same pine tree host were pooled, frozen, and lyophilized. DNA was extracted using a modified Qiagen DNAeasy Bloog and Tissue Kit. Samples were PCRed for the ITS1 region with ITS1F and ITS2 primer pair. PCR products were cleaned with AMpure beads, quantified with qubit, and pooled to equimolar concentration before shipping to be sequenced at the University of California, Berkeley QB3 Vincent J Coates Genomics Sequencing Laboratory"