title: NGS of RNA derived from defensive glands' tissue of P. cochleariae larvae after the injection of Pcsut6-dsRNA into the body cavity (second biological replicate)
design: dsRNA of Pcsut6 was injected into the body cavity of Phaedon cochleariae larvae. Several days later, the larvae were prepared (second biological replicate). The tissues derived from the defensive glands of 5 larvae were pooled and the RNA was extracted. A cDNA library was generated and sequenced using an Illumina HiSeq2500 in single-read mode (50bp). All reads were extracted in FastQ format. Subsequently, mapping onto P. cochleariae's transcriptome and expression studies are planned.