title: GSM4217309: Drd1-EGFP/Rpl10a (CP73) dorsal striatum older mouse IP Rep3 [1612]; Mus musculus; RIP-Seq
library_construction_protocol: The dorsal striatum and accumbens nucleus were dissected  and homogenized and tagged polysomes were immunopurified with monoclonal anti-EGFP antibodies bound to biotinylated-Protein L (Thermo Fisher) coated streptavidin-conjugated magnetic beads (Life Technologies). Total RNA from IPs was purified with the Agilent Nanoprep kit following manufacturer's protocol. RNA was extracted and treated as described in PMID 24810037, Heiman M, Kulicke R, Fenster RJ, Greengard P, Heintz N. (2014) Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nat Protoc. 9:1282-1291
attributes.tag: GEO Accession
attributes.value: GSM4217309