title: WGS of archaeological Native American subadult skeleton: Tlatelolco site (A.D. 900-1520)
design: Double-stranded Illumina sequencing libraries for shotgun sequencing were built using the protocol by Meyer and Kircher (2010) and modified according to Fortes and Paijmans (2015, Methods Mol. Biol. 1347, 179 195). Three microlitres of the resulting libraries were amplified and indexed in a 25 l reaction containing 1X AmpliTaq Buffer, 2mM MgCl2, 0.1 mg/ml BSA, 0.25mM dNTPs, 1.25U AmpliTaq Gold 360,  0.2 M IS4 Forward Primer and 0.2 M each of individually barcoded P7 Indexing Primer. The libraries were constructed using an 8 bp barcode on the 3' end of the P5 adapter (directly adjacent to the 5' end of the aDNA template), which served as an additional means to assign sequences to samples (as described in Fortes and Paijman 2015). The 8bp barcode for this sample is CCAGCTGG and has not been removed from the raw reads.