title: GSM2897925: Repop D5; Mus musculus; RNA-Seq
library_construction_protocol: C57BL/6J mouse was perfused by 20 mL L15 culture medium. Brain was quickly minced on ice. Then the tissue was dissociated with papain (Sigma) for 20 min at 37℃, followed by the termination of ovomucoid containing L15 culture medium (2 mg/mL). Next, myelin was removed from the single cell suspension by Percoll density gradient centrifugation (25% v/v, SolarBio). Then the cells were reacted with 1:250 PE rat anti-mouse CD11b (BD Pharmingen, 553311) and 1:250 APC anti-mouse CD45 (BD Pharmingen, 561018) on ice for 1 h. After FACS buffer rinse for 3 times, the cells were reacted with 7-AAD (1:80, BD Pharmingen, 559925) on ice for 10 min to exclude the dead cells. Microglia were sorted as CD11b+/CD45+ cells30 by BD FACS Aria III (BD Biosciences). Then the harvested cells were sent to prepare library through 10X genomics, followed by the pair-end 100 sequencing via Illumina HiSeq 4000. 609 cells from 4 CD-treated mice were sequenced at 0.544 M mean reads of sequencing depth per cell, while 585 cells from 6 mice of repopulation day 5 were sequenced at 0.564 M raw reads of sequencing depth per cell.
attributes.tag: GEO Accession
attributes.value: GSM2897925