title: GSM4550183: Spleen_KO_Treg_1; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were removed and ezymatically and mechanically dissociated using the mouse tumor dissociation kit from Miltenyi. Spleens were collected and mechanically dissociated by mashing them through a 40μm cell strainer. Mononuclear cells were isolated by density gradient centrifugation. Cells were stained with anti-mouse antibodies and regulatory T cells were isolated by fluorescence activated cell sortring. RNA was extracted using RNeasy plus micro kit (Qiagen) and ribosomal RNA was depleted using NEBnext ultra II rRNA depletion kit. Libraries were generated using NEBnext Ultra II RNA Library Prep Kit
attributes.tag: GEO Accession
attributes.value: GSM4550183