title: GSM4551270: P. falciparum 3D7 transcriptome at time point 21; Plasmodium falciparum; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol (Invitrogen) with chloroform supplemantaiton (Merck) and Direct-Zol-96 kit (Zymo) following manufacturers' instructions. All RNA samples were analyzed for integrity using Agilent Bioanalyzer 2100 platform. RNA 6000 Nano Chip (Cat. No.: 5067-1511, Agilent) was used for RNA samples according to manufacturer instructions. Reverse transcription and cDNA synthesis were done following modifed Smart-Seq2 protocol using only oligo(d)T primers to enrich for mRNA transcripts (Picelli, S., Faridani, O., Björklund, Å. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171–181 (2014)). Purified amplicon (cDNA) was used to generate sequencing libraries using Illumina Nextera XT kit as described in manufacturer's protocol. Libraries were analyzed on the Bioanalyser High-Sensitivity DNA chips (Agilent, Cat. No.: 5067-4626), subsequently pooled (24 samples per lane) and sequenced on Illumina HiSeq4000 platform generating 150 bp paired end reads with 110 Gb data output generated per lane.
attributes.tag: GEO Accession
attributes.value: GSM4551270