title: GSM3142618: DGRP-551_1d_WholeBrainNuclei_Unstranded_Rep2_RNA-seq; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, they were processed using the Nuclei EZ kit and RNA was isolated from collected nuclei using the Ambion RNAqueous-micro kit. Tn5 Mediated RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM3142618