title: PA-seq of VSMCs from 2 years old Rat
design: Aortic vascular smooth muscle cells were derived from 2 years old SD rat. Total RNA samples were isolated using TRIzol reagent (Life technologies, NY, USA). The PA-seq libraries were constructed by following the protocol published before by Ni et al. (BMC Genomics,2013). The concentration of the RNA-seq libraries was measured by Qubit fluorometry (Life Technologies, NY, USA) before loading onto the Illumina HiSeq2000 platform.