title: Illumina HiSeq 2000 sequencing; De novo and reference transcriptome assembly of transcripts expressed during flower development provide insight into the seed setting ability of tetraploid red clover (Trifolium pratense L.)
design: De novo and reference transcriptome assembly of transcripts expressed during flower development provide insight into the seed setting ability of tetraploid red clover (Trifolium pratense L.)
library_construction_protocol: flower buds collected from two genotypes of Tripo (low seed yielding variety) and Lasang (High seed yielding variety) at early, middle and late flowering stages RNA was extracted using the Sigma SpectrumTM Plant Total RNA Kit (Sigma Life Science).  On-Column DNase kit was used to remove the DNA contamination. TruSeq Stranded Total RNA Library Prep Kit was used for library preparation for each sample
attributes.tag: Experimental Factor: Tripo
attributes.value: cultivar
attributes.tag: Experimental Factor: middle flowering
attributes.value: developmental stage