title: Comparative transcriptomics of Yarrowia lipolytica on different substrates
design: Comparative transcriptomics of Yarrowia lipolytica on different substrates
library_construction_protocol: batch culture in minimal media (yeast nitrogen base, YNB) containing different carbon sources at 28 degrees C Cell samples were harvested in the middle log phase, filtered at 0.22 um and flash frozen in liquid nitrogen Frozen cells have been disrupted using Sartorius mikrodismembrator (2 min at 2600 rpm). Total RNA were purified using Rneasy kit from Qiagen according to manufacturer's protocol.
attributes.tag: Experimental Factor: GrowthCondition
attributes.value: YNB with 2% triolein