title: GSM3307242: G-13063 cell line TEAD4 KO; Homo sapiens; RNA-Seq
library_construction_protocol: GBM derived cell lines and gliomaspheres were resuspended in Trizol reagent (15596-026, Life Technologies) and flash-frozen. Total RNA was obtained following manufacturer's protocol, including a DNAse digestion step (Zymo Research). cDNA was generated using Clontech SMART-seq v4 (634888). Briefly, 10ng of RNA were converted to cDNA by introducing 3' SMART-Seq CDS Primer II A and the SMART-Seq v4 Oligonucleotide. cDNA was subsequently amplified in 8 cycles by LD PCR and purified by Agencourt AMPure XP Kit (Beckman Coulter A63880). Libraries were prepared using Nextera XT (FC-131-1024). Briefly, 1ng of pre-amplified cDNA was tagmented for 5 mins and subsequently amplified adding Index1 (i7) and Index2 (i5) adapters for 12 cycles. Libraries were then cleaned up using AMPure XP and pooled in equimolar conditions. Regular QC assays were applied before sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3307242