title: Albacore Genome Assembly and identification fo Sex Markers
design: For genomic sequencing, wild fish were sampled from Pacific Ocean. These specimens were collected via hook and line sampling by either private sport anglers or commercial/subsistence fishers aboard various fishing vessels, and scientific observers examining gonadal tissue identified phenotypic sex. Muscle, fin, or gonadal tissue was placed in 100% ethanol until DNA extraction.  Several hundred sexed-specimens were collected from these locations, and 40 of these fish (42 of each sex, for a total of 21 male and 21 female specimens), were selected to undergo sequencing based on tissue quantity and DNA quality. For all specimens, genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturers protocol. Paired-end libraries were prepared for 40 samples and mate-pair for 2 samples for sequencing. Each library was run in a single lane (total of four lanes) on the Illumina HiSeq 2500 sequencer (Illumina).