title: RNA-Seq of Bacillus subtilis 3610
design: B. subtilis 3610 was cultivated respectively in LB solid agar medium with and without 5 g/L sucrose after 1L inoculation in the center of the plate. Cultured at 35, two conditions bacterial cells in swarm edge were collected after 4 h and 18 h of incubation (as four groups). After the isolation of total RNA, cDNA library construction and Illumina Hiseq sequencing from the total RNA were completed