title: Kakapo captivity study-Post2
design: "DNA was extracted from samples as per methods specified in publication. Bacterial 16S rRNA gene fragments were amplified using primers 533f and 905r with standard Roche adapter/barcodes annealed to the forward primer, and adapter to the reverse. Sequencing was performed by Macrogen Inc. using the Roche 454 pyrosequencing platform (GS FLX Titanium chemistry). Raw data were then separated by barcode using mothur 1.31.0, allowing for 2 base mismatches in the primer sequence, and 1 mismatch in the barcode sequence."