title: GSM3307663: P2_4_6; Methylorubrum extorquens; RNA-Seq
library_construction_protocol: RNA was extracted from biomass using RNeasy kit (Qiagen) after cell lysis with lysozyme (Sigma-Aldrich) for 10 min and followed by homogenization with autoclaved 0.1 mm zirconia-silica beads (BioSpec) for 5 min. For gDNA contamination removal, DNA digestion with DNase (Qiagen) was performed thrice. Ribosomal RNA removal in RNA samples was done using Ribo-Zero Magnetic Kit (Illumina) and rRNA-depleted RNA samples were used as template to create cDNA libraries containing 250~300 bp inserts with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs).
attributes.tag: GEO Accession
attributes.value: GSM3307663