title: GSM1875550: Sample75.ReverbDKO.BMDM.RNA-seq.TGF.9h reads FPKM; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq: RNA was isolated using TRIzol LS (ThermoFisher Scientific) and resuspended with UltraPure water (ThermoFisher Scientific) supplemented with 1U/μL SUPERase (Ambion) treated with TURBO DNA-free kit (Ambion). Poly(A) selection was performed using the MicroPoly(A)Purist kit (Invitrogen) according to the manufacturer’s instructions. Poly(A) RNA was fragmented using RNA Fragmentation Reagents (Ambion) 10 min at 70°C and purified by running through a Micro Bio-Spin P-30 column (Bio-Rad) according to the manufacturer’s instructions. 30 nanograms RNA was utilized for subsequent library preparation.
attributes.tag: GEO Accession
attributes.value: GSM1875550