title: GSM1607382: yw_faire_input; Drosophila melanogaster; FAIRE-seq
library_construction_protocol: FAIRE-seq: Embryos ranging in age from 2.5 to 3 hours were collected, dechorionated, and crosslinked for 10 min with 3:1 heptane:fix solution at room temperature.  Following washing, embryos were flash frozen in liquid nitrogen and stored at -80°C.  FAIRE was performed on approximately 0.1 g of embryos, as previously described in McKay and Lieb (2013) with the following exceptions.  The homogenized embryos were filtered through Miracloth (EMD Millipore, Billerica, MA).  After resuspension in Lysis Buffer (10 mM Tris-Cl pH 8, 100 mM NaCl, 1 mM EDTA, 2% Triton X-100, 1% SDS), the nuclei were subjected to four rounds of bead-beating with 0.5mm glass beads.  The chromatin was sonicated three times for 30 seconds using a Branson Sonifier 250 at a power setting of 1.5 to achieve a size range of 200bp-1kb. FAIRE-seq libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM1607382