title: Metagenome or environmental sample from potato rhizosphere microbiome
design: degenerate primers A3F (5’ GCSTACSYSATSTACACSTCSGG 3’) and A7R (5’ SASGTCVCCSGTSCGGTAS 3’), and DNA coding for ketosyntase (KS) and methyl-malonyl-CoA transferase (met-mal-CoA) domains of PKS (between 1200 and 1400 bp) was amplified using degenerated primers K1F (5’ TSAAGTCSAACATCGGBCA 3’) and M6R (5’ TACTGGTACSGSAACCTGCG 3’). For amplification of DNA (~200 bp) partially encompassing the ACP domain of PKS type1 from Firmicutes, degenerate primers Pks firmi_F (5’ GCNGGNCAYWSNYTNGGNGARTAYA 3’) and Pks firmi_R (5’ CATRWANCKNSWRTGRAANGCNCC 3') were designed based on the most conserved regions of acyl carrier protein domains of bacillaene, difficidin and macrolactin.