title: Low coverage whole genome seq from IGROV-1
design: Shot-gun whole genome libraries were prepared using the KAPA  library preparation kit (KAPA Biosystems). Whole genome DNA libraries were  created, according to manufacturers instructions. Before end-repair, a  4-hour incubation step at 65C was added to remove as many reversible  crosslinks as possible, after which excessive single stranded DNA was removed  using Mung-Bean nuclease. The concentration of double-stranded DNA was  reassessed using Pico-green and the concentration of adapters used in the  ligation step of the library construction was modified based on the DNA  measured. For the library enrichment, 515 cycles of PCR with intermediate  assessment steps were used instead to ensure low adapter dimer content and  high library yield. Following quantification with qPCR, the resulting  libraries were sequenced on a HiSeq2500 (Illumina) at low coverage (1) for  shallow-seq