title: RNA-Seq (Total RNA) Fetal Adrenal Gland
design: Illumina TruSeq RNA PART# 15008136 REVISION A
attributes.tag: EXPERIMENT_TYPE
attributes.value: mRNA-Seq
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: Qiagen TissueLyser and RNeasy Lipid Tx Mini kit
attributes.tag: EXTRACTION_PROTOCOL_MRNA_ENRICHMENT
attributes.value: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
attributes.tag: RNA_PREPARATION_INITIAL_RNA_QNTY
attributes.value: 1ug
attributes.tag: RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE
attributes.value: Random Primers(hexamers)
attributes.tag: RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL
attributes.value: TruSeq RNA
attributes.tag: LIBRARY_GENERATION_PCR_TEMPLATE
attributes.value: cDNA
attributes.tag: LIBRARY_FRAGMENTATION
attributes.value: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
attributes.tag: LIBRARY_FRAGMENT_SIZE_RANGE
attributes.value: 120-200bp
attributes.tag: LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
attributes.value: Proprietary
attributes.tag: LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
attributes.value: 98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold
attributes.tag: LIBRARY_GENERATION_PCR_NUMBER_CYCLES
attributes.value: 15
attributes.tag: LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
attributes.value: Proprietary
attributes.tag: LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
attributes.value: Proprietary
attributes.tag: LIBRARY_GENERATION_PCR_PRIMER_CONC
attributes.value: Proprietary
attributes.tag: LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
attributes.value: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
attributes.tag: GEO Accession
attributes.value: GSM1060241