title: GSM3143966: 016_7_21_SS1_N729S506; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were lysed in Trizol LS (ThermoFisher), RNA was prepared using miRNeasy micro kit from Qiagen and quantified using real-time PCR Adapted from the Smart-seq2 protocol, an oligo-based capture of Poly-A tailed mRNA was carried out followed by a high-fidelity reverse transcription using locked nucleic acids (LNA)-oligos to obtain full-length transcripts. mRNA complementary (c)DNA strands were then amplified using a PCR-based method and sequencing adaptors incorporated into the cDNA fragments in a single step using Tn5 transposase technology (Nextera XT reagents and barcoded adaptors kits).
attributes.tag: GEO Accession
attributes.value: GSM3143966