title: Ion Torrent Sequencing data for E. coli O157:H7 16S rRNA gene
design: The 16S rRNA gene was first PCR amplified from IRMM-449 Escherichia coli O157:H7 EDL933 reference material using universal primers 27F and 1492.  The PCR amplicons were enzymatically fragmented and sequenced using the Ion One Touch 200 Template kit.