title: Sorghum bicolor:PI_454679
design: DNA from the plant material was isolated from 14-day seedlings by using the CTAB protocol. Genomic DNA was digested individually with ApeKI and bar-coded DNA of 96 accessions was pooled and sequenced with a Genome Analyzer II at Institute for Genomic Diversity at Cornell University. Sequencing data was separated for each accession on the basis of barcodes and aligned to the BTx623 sorghum reference genome version 1.4 by using BWA