title: GSM1611337: wt1; Mus musculus; RNA-Seq
library_construction_protocol: RNeasy Mini Kit RNA-sequencing was used for transcriptome analysis. Pancreatic islets from 4 animals were used in each genotype group. Total RNA was isolated from islets using the RNeasy Mini Kit (Qiagen) according to manufacturer’s protocol and cDNA for sequencing was synthesized using Ovation RNA-Seq System V2 (NuGEN Technologies, Inc.), 10 ng of total RNA was used. SOLiD DNA Fragment library kit (cDNA input 2 µg) was used to generate libraries and quality was controlled with the Agilent Bioanalyzer 2100 (Agilent Technologies) before sequencing. The libraries were marked with different barcodes and pooled together for the following template preparation. The automated SOLiD EZ Bead System and SOLiD EZ Bead E80 System Consumables (Life Technologies) were applied for the template preparation. For sequencing the SOLiD 5500xl System and paired end (75 bp forward and 35 bp reverse) chemistry for DNA sequencing was applied (Life Technologies).
attributes.tag: GEO Accession
attributes.value: GSM1611337