title: GSM3307656: mRNA_ET-06_non-Vh11; Mus musculus; RNA-Seq
library_construction_protocol: Splenic single-cell suspensions were prepared in magnetic-activated cell sorting (MACS) buffer (PBS/2mM EDTA/0.5%BSA) and naïve splenic B cells from 8–12 week-old WT C57BL/6 mice were purified by MACS. Non-B cells, B-1 cells, GC B cells and plasma cells were first labeled with biotinylated antibodies (BD Biosciences) to CD5 (53–7.3), CD11b (M1-70), CD43 (S7), CD95 (Jo2), CD138 (281-2), Gr-1 (RB6-8C5) and TER-119 (PK136) and subsequently with streptavidin-conjugated magnetic beads (Miltenyi Biotec). Purity of MACS-sorted naïve B cells was confirmed by flow cytometry (typically > 99% CD19+ cells). CLL cells were purified from primary tumors from IgH.TEmu mice using CD19+IgMb+ MACS purification. RNA-Seq: standard Illumina TruSeq library construction.
attributes.tag: GEO Accession
attributes.value: GSM3307656