title: Zea mays B73 corn-leaf aphids feeding time-course
design: Plants and growth conditions: Single seeds of B73 were planted 1.5 cm deep in a 7.6 x 7.6-cm plastic pots (200 cm3) filled with moistened maize mix [produced by combining 0.16 m3 Metro-Mix 360, 0.45 kg finely ground lime, 0.45 kg Peters Unimix. Plants were grown in growth chambers under a 16-h-light/8-h-dark photoperiod and 180 mMol photons/m2/s light intensity at constant 23 °C and 60% humidity for two weeks (V2-V3 stage).     Aphid assays: For the time course bioassay, ten adult corn leaf aphid (Rhopalosiphum maidis) were confined on the second true leaf of two-week-old seedlings for 2, 4, 8, 24, 48 or 96 hours using clip cages.     RNA extractions:  Tissue from five individual maize plants was combined into one experimental replicate and five replicates were collected for each time point of the experiment.RNA was extracted using TRI Reagent and was purified with the SV total RNA isolation kit with on-column DNase treatment. The purity of total RNA extracted was determined in NanoDrop 2000 (Thermo Scientific), and 2-3 μg total RNA was used for preparation of strand-specific RNASeq libraries.