title: Lasiurus Illumina fastq
design: We extracted total genomic DNA from wing punches using Qiagen DNeasy Blood and Tissue kits. DNA was quantified using a Qubit fluorometer, and normalized to a concentration of 10 ng/*l. Samples were then shipped to the University of Minnesota Genomics Center (http://www.bmgc.umn.edu/index.htm) for library construction and sequencing. Briefly, DNA was sheared using a Covaris ultrasonicator, followed by fragment purification, end polishing, and ligation of indexed adaptors. Indexed libraries were then pooled, clustered on a single flow cell lane of an Illumina HiSeq2000, and sequenced to a target depth of 80 million 2 x 100 bp paired-end reads.  The mean insert size of libraries was 250 bp.