title: control_pups
design: The amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche).  #SampleID BarcodeSequence LinkerPrimerSequence ReversePrimer Description  NOD.01. TCACGTACTA CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.02 CGTCTAGTAC CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.03 TCTACGTAGC CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.04 TGTACTACTC CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.05 ACGACTACAG CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.07 CGTAGACTAG CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.08 TACGAGTATG CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.09 TACTCTCGTG CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.11 TAGAGACGAG CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.12 TCGTCGCTCG CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.13 ACATACGCGT CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.14 ACGCGAGTAT CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.15 ACTACTATGT CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.16 ACTGTACAGT CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.17 AGACTATACT CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT  NOD.20 AGCGTCGTCT CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT