title: Illumina HiSeq 2000 sequencing; Direct RNA Sequencing of polyadenylated RNA & high-depth Illumina strand-specific, short read RNA-seq of a stably RNAi-silenced nuclear 5'-3' exonuclease XRN3 mutant line in Arabidopsis thaliana
design: Direct RNA Sequencing of polyadenylated RNA & high-depth Illumina strand-specific, short read RNA-seq of a stably RNAi-silenced nuclear 5'-3' exonuclease XRN3 mutant line in Arabidopsis thaliana
library_construction_protocol: Seedlings were collected after 14 days of growth. Seeds were surface-sterilized, stratified and grown on Murashige and Skoog (MS) medium supplemented with 1% (w/v) sucrose and 0.3% phytagel, under a 16 h light/8 h dark photoperiod. Total RNA was extracted using Trizol reagent (Sigma). Small RNA Sample Prep Kit v1.5, including PAGE selection of small RNAs (18-30 nt).
attributes.tag: Experimental Factor: wild type genotype
attributes.value: genotype
attributes.tag: Experimental Factor: small RNAs
attributes.value: fraction
attributes.tag: Experimental Factor: Illumina 50bp single-end sequencing
attributes.value: protocol