title: GSM4217845: Retina_P1; Mus musculus; miRNA-Seq
library_construction_protocol: After the mice were euthanized, the retinas were isolated from the dissected eye and moved into the RNA-Solv Reagent (Omega Bio-tek, Norcross, GA, USA). Retinas from 5 eyes were pooled as one sample. T10 basic S25 ULTRA-TURRAX Disperser (IKA, Guangzhou, China) was used to homogenize retinas. Total RNA was extracted by miRNA Kit (200) (Omega Bio-tek) according to the manufacturer's instructions. The quantity and integrity of RNA yield was assessed by using the Qubit®2.0 (Life Technologies, USA) and Agilent 2200 TapeStation (Agilent Technologies, USA) separately. The total RNA (1 μg) of each samples were used to prepare small RNA libraries by NEBNext® Multiplex Small RNA Library Prep Set for Illumina (NEB,USA) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4217845