title: GSM1608385: 77_MNase-Exo_limit-digest-1; Saccharomyces cerevisiae; MNase-Seq
library_construction_protocol: Nuclei were prepared from unfixed yeast cells and then digested with a range of MNase concentrations at two different ExoIII concentrations. In vitro reconstitutions were performed by mixing recombinant yeast histone octamers with purified plasmid pRS-ARG1-B DNA followed by salt dialysis. In both cases, mono-nucleosomal DNA was gel-purified (~150 bp). See paper for details. The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.
attributes.tag: GEO Accession
attributes.value: GSM1608385