title: GSM4117822: RNAAtlas210_polyA_RNA; Homo sapiens; RNA-Seq
library_construction_protocol: RNA of individual cell types was obtained from ScienCell Research Laboratories or isolated from cell types collected at Ghent University Hospital. RNA from collected cell types and (cancer) cell lines was isolated using the miRNeasy kit (Qiagen) according to the manufacturer's instructions. RNA samples from normal human tissues were obtained from Ambion and Biochain. Small RNA libraries were generated using the TruSeq Small RNA library prep kit (Illumina) according to the manufacturer's instructions, using 750 ng input RNA. Library size selection was performed using a Pippin Prep device (Sage Science). Total RNA libraries were generated using the TruSeq stranded total-RNA library prep kit with Ribo-Zero Gold (Illumina) according to the manufacturer's instructions using 1 µg of input RNA. PolyA RNA libraries were generated using the TruSeq Stranded mRNA library prep kit (Illumina) according to the manufacturer's instructions using 1 µg of input RNA.
attributes.tag: GEO Accession
attributes.value: GSM4117822