title: DNA-Sequencing of seawater from the Florida Keys, FL: COI target markers
design: This protocol is aimed at amplifying the cytochrome oxidase I gene (COI) in eukaryotes. The primers (mlCOIintF,   HCO2198) utilized in this protocol are based on the primer utilized in Folmer et al. 1994 and Leray et al. 2013.