title: medaka d-rR blastula RNA-seq
design: RNA was isolated from medaka blastula using ISOGEN (Nippon Gene). Purified RNA were treated with Ribominus eukaryote kit for RNA-seq (Life Technologies). RNA-seq library was prepared using TruSeq RNA-seq sample prep kit (Illumina). The PCR products were purified and size fractionated using a bead-mediated method (AMPure, Ambion). Sequencing was conducted on HiSeq 2000 platform (Illumina).