title: GSM3307684: Danio_rerio_ChIPseq_spikein_3dpf_rnf2_r2; Danio rerio; ChIP-Seq
library_construction_protocol: ChIP-seq: Embryos of 3 dpf were fixed, deyolked, and homogenized using pestles and sonication to isolate the chromatin ChiP-Seq: For spike-in experiments zebrafish chromatin was mixed with Drosophila spike-in chromatin and incubated overnight with H2Aγ and RING1B antibodies (H2Aγ Active Motif 104597; RING1B cell signaling D22F2) or with H2Aγ and H3K27me3 antibodies (H2Aγ Active Motif 104597; Millipore 07-449). In the regular non-spike in experiment zebrafish chromatin was  incubated with the H3K27me3 antibody overnight (Millipore 07-449). After o/n antibody incubation the chromatin was extensive washed. The resulting ChIP-DNA was used as input for KAPA-HYPERprep library preparation
attributes.tag: GEO Accession
attributes.value: GSM3307684