title: GSM2474529: ADAR1 WT MDA5 knockout Adult Brain - Whole. Replicate 3 (84B); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the whole brain from 3 independent biological replicates from 12 week old male Adar1+/+Ifih1-/- and Adar1E861A/E861AIfih1-/- mice. Post ribosome depleted RNA was purified and subjected to indexing and library preparation using the Kapa Stranded RNA-seq Library Preparation Kit for Illumina Platforms (Kapa Biosystems). Library amplification was performed for 9 cycles of PCR. The resultant libraries were pooled and sequenced on a single run using the Illumina NextSeq500 with 75bp paired end reads at the Ramaciotti Centre for Genomics (UNSW, Australia).
attributes.tag: GEO Accession
attributes.value: GSM2474529