title: NextSeq 500 paired end sequencing; RNAseq of proneural glioblastoma stem-like cells 72 hours after infection with human cytomegalovirus (CMV) Towne strain
design: RNAseq of proneural glioblastoma stem-like cells 72 hours after infection with human cytomegalovirus (CMV) Towne strain
library_construction_protocol: Samples were cells in culture, centrifuged and pellets collected RNA was extracted from cell pellets using Trizol as described by the manufacturer. RNA degradation and contamination were monitored on 1% agarose gels and RNA integrity was assessed with the RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system  For the infection studies triplicates of proneural GSCs (G44) cultured in suspension in neurobasal medium plus B27 supplement in 24 well plates were infected with HCMV Towne strain at a multiplicity of infection of 0.1. Cells were then kept in culture for three days. Uninfected cells served as controls. The libraries were prepared from 500ng of total RNA using Illumina TruSeq strand specific mRNAseq library prep kit according to manufacturer's protocol.
attributes.tag: Experimental Factor: infect
attributes.value: human cytomegalovirus (Towne strain)