title: Bulk WGS of Resistant Clone of 451Lu
design: Bulk DNA was extracted using Qiagen Kit. 1ug of DNA was sheared by sonication. Libraries for sequencing were prepared by subjecting the resulting DNA fragments to end-repair, 3_ adenylation and ligation of TruSeq barcoded adapters. After ligation, DNA fragments were size selected using Ampure XP beads. A 0.75 ratio of beads to sample was used to select beads larger than 200bp and avoid adapter dimers. These fragments were further amplified by PCR, cleaned using Qiaquick PCR Purification Kit and then once again size selected using Ampure XP beads with a 0.75 ratio of beads to sample. The final libraries were then quantified on Nanodrop and Bioanalyzer using Agilent's High Sensitivity DNA Analysis Kit. 10nM of each sample was then pooled together and the resulting pool was then sequenced single read 76bp length on NextSeq