title: Sequencing of the SSR-enrichment genomic library of Nilaparvata lugens
design: SSR-enrichment genomic library of  Nilaparvata lugens was created and sequenced.
library_construction_protocol: The SSR-enrichment genomic libraries was prepared from Hadano-66 genomic DNA, which was extracted from approximately 50 insects, including nymphs, adults, males, and females. The fast isolation by amplified fragment length polymorphism (AFLP) of sequences containing repeats (FIASCO) procedure described by Zane et al.(2004) was used to isolate the library at the Plant Breeding Laboratory (Kyushu University). Briefly, genomic DNA was digested with MseI and ligated to MseI adaptors (5′–TACTCAGGACTCAT–3 /5–GACGATGAGTCCTGAG–3′). The mixture was amplified with an MseI adaptor primer (5′–GATGAGTCCTGAGTAAN–3′). The PCR product was then hybridized with 6 biotinylated probes: (CT)10, (CA)10, (CAC)7, (AAG)7, (TCC)7, and (GACA)5. The DNA molecules that were hybridized to biotinylated probes were captured using streptavidin magnetic paticles (Roche Applied Science, Mannheim, Germany) using a magnetic field. PCR was performed to recover enriched DNA fragments using the MseI adaptor primer.