title: GSM2817822: negative_2f; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol (Invitrogen) per the manufacturer's protocol.  Purified RNA was subjected to polyA selection using the NEBNext Polyadenylated mRNA Magnetic Isolation Module and subsequently used for library preparation with the NEBNext Ultra RNA Library Prep Kit (both from New England Biolabs). Libraries were analyzed on a Tapestation (Agilent) for quality control, quantified using the Qubit dsDNA HS Assay (Invitrogen) and equimolar pools were sequenced on HiSeq 2500 using 100-bp single-end protocols. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2817822