title: MBD-seq 9wk sample 181
design: Female mice were kept on either a high or low fat diet for 5 weeks prior to mating and through weaning. At weaning, male mice from each litter were fed either a high or low fat diet. We sheared DNA samples to ~200 bp and incubated them with protein G beads coated with recombinant MBD proteins. After washing, we eluted densely methylated DNA molecules to prepare Illumina sequencing libraries according to the manufacturer’s instructions. We sequenced each library (n=8) on one lane of a Genome Analyzer IIx to generate 27-35 million reads of 36 bp.