title: GSM1262357: Meis2 P2 4C-seq; Mus musculus; OTHER
library_construction_protocol: Lysates were clarified from EcoRI digested nuclei and made 3C reaction. Libraries were prepared by primer extention with biotinylated primer. Primer extention product was conjugated to streptoavidine beads and digested with NlaIII. After adaprtor ligation, 2 rounds of nesting PCR were carried out to adjust the library for highthroughput sequencing by Illumina MiSeq.
attributes.tag: GEO Accession
attributes.value: GSM1262357