title: bisulfite sequencing of CENH3 nucleosome DNA
design: Chromatin was fragmented with micrococcal nuclease to predominantly single nucleosome cores (but also including larger fragements). Chromatin immunoprecipitation was used to enrich for CENH3 containing nucleosomes. The DNA fragments were ligated to premethylated Illumina adapters then treated with sodium bisulfite and sequenced.