title: GSM1254466: WT seedlling at 20°C; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated from liquid nitrogen pulverized tissues. cDNA libraries preparation on ribosomal RNA depleted transcripts, high-throughput sequencing, quality control and bio-informatic analyses of the RNA-seq were performed by FASTERIS Life Sciences (http://www.fasteris.com) cDNA libraries preparation on ribosomal RNA depleted transcripts, high-throughput sequencing, quality control and bio-informatic analyses of the RNA-seq were performed by FASTERIS Life Sciences (http://www.fasteris.com) The RNA-seq and differential accumulation analyses were conducted by Fasteris SA as follow: ribosomal RNAs were removed using using duplex-specific nuclease (DSN) from 0.5-1mg of total RNA, after zinc breaking cDNA libraries were prepared and sequenced as single-end 100 bp tags with an Illumina Hi-seq 2000 instrument.
attributes.tag: GEO Accession
attributes.value: GSM1254466