title: PacBio whole genome sequencing of Azolla filliculoides: whole plant
design: Libraries for PacBioRS II (Pacific Biosciences, Palo Alto, CA,USA) sequencing of the nuclear DNA from a single plant of A. filiculoides-Sterilized were generated after size separation with a cut-off at14 kb (Blue Pippin, Sage Science, Beverly, MA, USA) accordingto the PacBio RS II protocol and sequenced using P5-C3 chemistry and 43 SMRT cells.