title: GSM4276583: E12.5-WT-rep2 [ATAC-seq]; Mus musculus; ATAC-seq
library_construction_protocol: The GFP-positive anterior palatal shelves  were isolated from E12.5  Wnt1Cre;Meis2f/+;R26RmTmG and  Wnt1Cre;Meis2f/f;R26RmTmG embryos. Samples  were subjected to digestion by a cocktail of 0.02%  collagenase I, II and IV in HBSS for 30min,  followed by Accutase solution (Sigma) treatment  for 5 min at 37℃. After stop of the digestion with  culture medium containing 10% fetal bovine  serum (FBS), cells were washed with PBS  containing 3% FBS and then filtered through a 70-  μm filter. Suspended cells were sorted by  Fluorescence-activated Cell Sorting (FACS) on a  BD FACS Aria Fusion cell sorter (Becton-  Dickinson). Around 50,000 single GFP-positive  cells from each group were obtained and were  subjected promptly to generation of ATAC-seq  library with the Nextera DNA Library Preparation Kit
attributes.tag: GEO Accession
attributes.value: GSM4276583