title: Loki GC14 75 non-amplified DNA 16S amplicon library
design: DNA was extracted from 7,5 gram sediment using the FastDNA spin kit for soil in conjunction with the FastPrep-24 instrument (MP Biomedicals) following manufacturer’s protocol, except for the addition of polyadenosine as described by Hugenholtz et al. The individual extractions were then pooled and up-concentrated to a final volume of 50 µl using Amicon Ultra-0.5 filters (50.000 NMWL) following the manufacturer’s protocol (Merck Millipore).  16S amplicons (~900 bp) were generated by PCR, using 'universal' primer pairs A519F and U1391R. PCR products were purified  and a library was prepared using the Illumina TruSeq DNA LT Sample Prep Kit, and sequenced on Illumina MiSeq instrument, set to 2x300 bp mode.