title: RNA-Seq of Asian seabass: adult transitioning gonad stage 2, biological replicate 1
design: Total RNA was extracted using the RNeasy mini kit (Qiagen). RNA concentration and integrity was measured on the NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific) and visually assessed by agarose gel electrophoresis with ethidium bromide staining. RNA was stored at -80 C. The Illumina Truseq stranded total RNA preparation protocol was used to generate cDNA libraries. 150 nt single-end sequencing was performed on the NextSeq 500.