title: Thiolkalivibrio versutus D301 RNASeq
design: Transcriptome sequencing protocol included RNA extraction, RNA sample quality control, library construction, purification and quantification, cluster generation and finally sequencing. Total RNA was extracted, purified, Dnase-treated and quantified. RNA integrity was assessed using 2100 Bioanalyzer Instrument and RNA 6000 Nano Kit (Agilent Technologies, The Netherlands). Paired- end libraries were constructed, purified and quantified using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, UK), AxyPrep MagTM PCR Clean-up Kit (Axygen Biosciences, USA) and High Sensitivity DNA Kit (Agilent Technologies, The Netherlands) and Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA), respectively. Automatic cluster generation and sequencing were performed using cBot 2 System and TruSeq PE Cluster Kit v3 cBot HS, HiSeq 2500 System using TruSeq SBS Kit v4 HS (Illumina, USA), respectively.