title: Illumina-insect
design: RNA was treated with DNase I (Invitrogen,Carlsbad, CA, USA) to remove any potential genomicDNA contamination. RNA degradation and contaminationwas monitored on 1% agarose gels. RNA puritywas checked using the NanoPhotometer spectrophotometer(IMPLEN, CA, USA). RNA concentration wasmeasured using Qubit RNA Assay Kit in Qubit 2.0Flurometer (Life Technologies, CA, USA). RNA integritywas assessed using the RNA Nano 6000 Assay Kitof the Bioanalyzer 2100 system (Agilent Technologies,CA, USA).