title: GSM2055494: L. major H3V-KO + DMOG rep 1; Leishmania major; RNA-Seq
library_construction_protocol: Total RNA was isolated from log phase Leishmania major cultures using Trizol, according to the manufacturer’s instructions. 5x107 cells were used for each prep. RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit following the manufacturer’s instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 µg, and all volumes were reduced to a third of the described quantity.
attributes.tag: GEO Accession
attributes.value: GSM2055494