title: GSM3142783: Capped nuclear RNA-seq of wild-type young adults, replicate 1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Nuclei were isolated and then chromatin associated RNA (development series) or nuclear RNA (ageing series) was isolated. Chromatin associated RNA was isolated as in (Pandya-Jones and Black 2009), resuspending washed nuclei in Trizol for RNA extraction. To isolate nuclear RNA, nuclei were directly mixed with Trizol. Following purification, RNA >200nt was isolated using Zymo clean and concentrate columns. RNA was treated with DNase I, then with 5' Terminator nuclease (Epibio) to remove rRNA, and then stranded libraries prepared using the NEB Next Ultra Directional RNA Library Prep Kit (#E7420S). Libraries were made from two biological replicates for each developmental stage and each ageing time point. RNA-seq of >200nt capped nuclear RNA
attributes.tag: GEO Accession
attributes.value: GSM3142783