title: Ion Torrent Sequencing data for Listeria monocytogenes 4b NCTC 19944 16S rRNA gene
design: The 16S rRNA gene was first PCR amplified from IRMM-447 Listeria monocytogenes strain 4b NCTC 19944 reference material using universal primers 27F and 1492. The PCR amplicons were enzymatically fragmented and sequenced using the Ion One Touch 200 Template kit and 316 chip.