title: GSM4550588: Col-0 Rep3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted with Spectrum plant total RNA kit (Sigma Aldrich) from 4-5 week old leaves of WT Col-0, F1 DDM1/ddm1 (Col-0Xddm1) and ddm1 2nd generation. Contaminating DNA was removed with a DNA-free DNA removal kit (Thermo Fisher Scientific). For RNA-seq analyses, rRNA was removed by Ribo-Zero rRNA Removal kit (Illumina). After depletion of rRNA, RNA-seq libraries were generated with NEBNext UltraII directional RNA library prep kit for Illumina (New England Biolabs) following the manufacturer's instructions. The libraries were sequenced with Illumina Hiseq 2500 to generate single-end 50 bp reads. Samples were prepared from three independent biological replicates.
attributes.tag: GEO Accession
attributes.value: GSM4550588