title: GSM1257668: Hippocampus_8mon 4; Mus musculus; RNA-Seq
library_construction_protocol: RNA samples were isolated fromhippocampus of mouse brain region in TRI-Reagent (Sigma-Aldrich, St. Louis, MO) and total RNA was isolated using a phenol/chloroform protocol (Sigma-Aldrich). For the construction of micro RNA libraries using the IonTotal RNA-Seq Kit v2 and Ion Xpress™ RNA-Seq Barcode kit(Lifetechnologies, USA).The  Ion Total RNA-Seq Kit v2 (Lifetechnologies, USA) was used for the preparation of micro RNA libraries according to the manufacturer's instructions starting with 100ng total RNA. Template is prepared from the libraries using the Ion OneTouch™ System (Lifetechnologies, USA) and sequenced on the Ion PGM™ Sequencer (Lifetechnologies, USA).
attributes.tag: GEO Accession
attributes.value: GSM1257668