title: GSM4217370: Flower buds, ChIP-seq_date1_2_K4; Prunus avium; ChIP-Seq
library_construction_protocol: For ChIP-seq, ChIP was conducted on 300-500 mg of frozen and pulverized flower buds using the procedure described in Leida et al. (2012) with minor modifications (1% PVP-40was added to the different buffers and DNA extraction and purification was carried out using SPRI beads). For immunoprecipitation , we used anti-trimethyl-histone 3 Lys 4 antibody (Millipore cat#17-614). For ChIP-seq libraries, library preparation was performed using all the volume for IPs (50µL) and 20 ng in 50µL using the TruSeq ChIP Library Preparation kit (48 samples, 12 indexes, Illumina, cat. no. IP-202-1012). DNA quality from libraries was evaluated using Tapestation 4200. The libraries were sequenced on a NextSeq500, at the Sainsbury Laboratory Cambridge University (SLCU), using paired-end sequencing of 75 bp in length.
attributes.tag: GEO Accession
attributes.value: GSM4217370