title: GSM3142567: SMART_seq2_R23E10_Cell_39; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 2 hours in a mix of Collagenase (75µl @ 100mg/ml) and Dispase (50µl @ 3mg/ml), the sample was pipette mixed every 15 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Cells were sorted into single wells containing SMART-seq lysis buffer. SMART-Seq
attributes.tag: GEO Accession
attributes.value: GSM3142567