title: ETMR6-rna_good
design: Samples were homogenized in nitrogen using mortal and pestle. Brain powder was immersed in TRIzol (Invitrogen) and subjected to a second round of homogenization using a POLYTRON for 30 seconds. Total RNA was extracted using miRNeasy kit (including a DNaseI treatment step) and quality assessed by Agilent 2100 BioAnalyzer (Agilent Technologies).  Libraries  were prepared according to the Illumina TruSeq protocols with the Gold option and strand-specific preparation. Quality of the libraries was assessed using Agilent 2100 BioAnalyzer (Agilent Technologies). Samples were indexed (3 or 4 per line) and sequenced in an Illumina HiSeq 2000.