title: GSM2055352: mRNAseq_16wkHFD_rep1; Mus musculus; RNA-Seq
library_construction_protocol: We euthanized all mice at 24 weeks after an overnight fast and froze the livers prior to removal using clamps cooled in liquid nitrogen. The frozen livers were then pulverized into a powder using a CryoPREP impactor (Covaris). We prepared aliquots of pulverized liver for all samples and used these for subsequent library construction. We extracted total RNA from the pulverized frozen livers of mice using the miRNeasy Mini kit (Qiagen). We prepared mRNA-Seq libraries using the TruSeq RNA Sample Prep Kit v1 (Illumina) and size-selected using 2% agarose gel electrophoresis for 180 +/- 25 base-pairs of insert. We multiplexed mRNA-Seq libraries and paired-end sequenced samples for 40-50 base-pairs on an Illumina Hi-Seq 2000 machine.
attributes.tag: GEO Accession
attributes.value: GSM2055352