title: GSM2816767: mock_mRNASeq; Homo sapiens; RNA-Seq
library_construction_protocol: 1X10^6 Low-passage HBTEC were mock-infected or infected with Ad5 vectors 3 days after reaching confluence. RNA was isolated 24h p.i. using QIAGEN RNeasy Plus Mini Kit. Eluted RNA was treated with Ambion DNA-free DNA Removal Kit and then Ambion TRIzol reagent, precipitated with isopropanol, and dissolved in sterile water. RNA concentration was measured with a Qubit fluorometer. One microgram of RNA was fragmented and copied into DNA then PCR amplified with bar-coded primers for separate samples to prepare sequencing libraries using the Illumina TruSeq RNA Sample Preparation procedure.
attributes.tag: GEO Accession
attributes.value: GSM2816767