title: GSM1690028: Dnmt3l-KO-Rep1 (T8); Mus musculus; RNA-Seq
library_construction_protocol: the C57BL/6 (B6) wild-type and Dnmt3l-KO MEF cell lines were derived from day 13.5-14.5 embryos via Dnmt3l heterozygote (Dnmt3l+/-) intercrosses rRNA-free RNAs were fragmented into small pieces, copied into first-strand cDNA using random primers, followed by second-strand cDNA synthesis, a single A base addition and adapter ligation using a TruSeq RNA library preparation kit (Illumina, San Diego, CA). The products were then enriched through 12 cycles PCR amplification to create the final cDNA library.
attributes.tag: GEO Accession
attributes.value: GSM1690028