title: mRNA extracted from Chunee hydroponic roots
design: A hydroponic system was established for free diazotrophic bacteria plant material. SP70-1143 (SP) and Chunee (CH) plants from sterile in vitro culture of meristems (provided by Centro de Tecnologia Canavieira - CTC) were transferred to Hoagland`s solution and the material was collected 30 days after transfer to hydroponic culture. Root and shoot tissues were collected separately, and before freezing, samples were washed in water / alcohol / water (1 minute for each). All collected samples were frozen in liquid nitrogen and stored in a freezer at -80*C until extraction. For RNAseq libraries construction, samples collected from fifteen plantlets were pooled and total RNA was extracted from frozen material according to Logeman et al. (1987). The quality and quantification of RNA samples was checked via agarose gel and Agilent 2100 Bioanalyzer TM (Agilent Technologies, Aplo Alto, CA). Two biological replicates of each plant material were used for RNAseq Illumina sequencing at FASTERIS (Switzerland).