title: GSM1264689: 35S::BBM; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Somatic embryo material was cross-linked using formaldehyde and ground prior to nuclear extraction. The nuclear pellet was sonicated and the lysate was used for immunoprecipitation with the µMACS GFP Isolation Kit (Miltenyi). After proteinase K treatment, the DNA was purified using the QIAquick kit (Qiagen). The Illumina ChIP-Seq library preparation kit was used for library construction using the manufacturer's protocol, except that 1:10 dilutions were used of the adapters. Library fragments of 200-500 bp were excised from the gel and purified.
attributes.tag: GEO Accession
attributes.value: GSM1264689