title: Sequnece Capture of Syntenic wheat W7894 (M6)
design: DNA was extracted from the 3 week-old seedlings using DNeasy Plant Maxi Kit (Qiagen). The DNA quality was assessed by agarose gel electrophoresis and quantification was performed with Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) using the Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek). The A260/280 and A260/230 ratios were measured on the NanoDrop ND-1000 Spectrophotometer (Thermo Scientific).   One µg of pooled DNA (250 ng or 125 ng of each component DNA sample library were used for 4x or 8x pools, respectively) was used in each of the sequence capture hybridizations. After sequence capture hybridization completion, captured samples were recovered, washed, amplified using LM-PCR, and purified according to the Plant Sequence Capture Illumina Optimized User’s Guide (Roche NimbleGen). The Post-Capture LM-PCR products were analyzed using the Bioanalyzer DNA 7500 chips and quantified with the Qubit DNA Broad Range (BR) assay kit.  The A260/280 and A260/230 ratios were measured on the NanoDrop ND-1000 Spectrophotometer (Thermo Scientific). If samples met the described above requirements, they were diluted to 2 nM concentration and submitted for sequencing.