title: GSM1258055: WT_14_rep1; Escherichia coli K-12; RNA-Seq
library_construction_protocol: All samples were extracted using the Qiagen Rnasey Mini Kit, following standard protocol cDNA libraries were constructed at Purdue using an adapted SOLiD Total RNA-Seq Kit. Total RNA (DNase I digested) was fragmented by RNase III. RNA samples labeled as TEX were also subsequently digested with Terminator Exonuclease (TEX). Pyrophosphate groups were removed from the 5′ terminus using tobacco acid pyrophosphatase (TAP), and an RNA adapter was ligated to the 5′ end of the RNA. First-strand synthesis was performed using standard SOLiD 4 Total RNA-Seq protocol. RNA-seq via SOLiD 4 sequencing of libraries prepared by ligation based chemistry to provide strand-specific datasets.
attributes.tag: GEO Accession
attributes.value: GSM1258055