title: GSM2897832: ATACseq_WT_rep2; Arabidopsis thaliana; ATAC-seq
library_construction_protocol: 1g of 12-14 days post germination seedling material from two biological replicates each of WT or suvh1/3 mutant genotypes as input. Briefly, seedling tissue was chopped on glass using a razor blade in 5 ml of ice-cold Lysis buffer (15mM Tris-Cl pH 7.5, 0.5mM spermine-4HCl, 80mM KCl, 20mM NaCl, 0.25 % Triton X-100, 5mM β-mercaptoethanol), filtered through 40µM strainer (Falcon) into 5ml Sucrose buffer (20mM Tris-Cl pH 8.0, 1.7M Sucrose, 5mM MgCl2, 0.25 % Triton X-100, 2mM EDTA, 5mM β-mercaptoethanol), and spun at 20,000xg, for 30mins at 4oC. Pellets were resuspended in 1ml of Lysis buffer and stained with DAPI (0.5µg/ml final concentration) and 50k nuclei were collected by FACS into 0.5ml of Lysis buffer. Tn5 transposase reaction followed by NEBNext High-Fidelity Master-Mix amplification.
attributes.tag: GEO Accession
attributes.value: GSM2897832