title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of human lung cancer cell line A549-NC and A549-SLC2A5 knockout xenograft in balb/c nude mice.
design: RNA-seq of human lung cancer cell line A549-NC and A549-SLC2A5 knockout xenograft in balb/c nude mice.
library_construction_protocol: RNA was collected from A549-NC/A549-SLC2A5KO tumor tissue by Ultrapure RNA kit according to the manufacturer's instructions. Total RNA (1 μg) was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, San Diego, CA, USA) following the manufacturer's instructions. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA,  USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).
attributes.tag: Experimental Factor: genotype
attributes.value: SLC2A5 knockout mediated by CRISPR/Cas9