title: Transcriptome sequencing of Kalopanax septemlobus embryogenic callus
design: Mature seeds of K. septemlobus were collected at August 20, 2012 from adult trees. These were immersed in 70% EtOH for 1 min, surface-disinfected in 2% NaOCl for 20 min, and rinsed five times with aseptic distilled water. The seed coat carefully dehusked under a microscope, and naked seeds were cultured in 10 x 2cm plastic petri dishes containing callus induction medium [7] of 25 ml MS medium [25] with 3% sucrose, 0.3% Gelrite, and 4.4 M 2,4-dichlorophenoxyacetic acid (2,4-D) to induce embryogenic callus. The culture room was maintained at 25*1*C in darkness condition. Embryogenic callus was induced within 6~8 weeks in culture and proliferated in CIM with 3 weeks culture interval until used. Samples were frozen in liquid nitrogen immediately after harvesting and stored at -80*C for further analysis.