title: c-section pups
design: The amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche). #SampleID BarcodeSequence LinkerPrimerSequence ReversePrimer C_sec_01 ACAGTATATA CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_02 ACGCGATCGA CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_03 ACTAGCAGTA CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_04 ACGAGTGCGT  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_05 ACGCTCGACA  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_06 AGACGCACTC  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_07 AGCACTGTAG  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_08 ATCAGACACG  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_09 ATATCGCGAG  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_10 CGTGTCTCTA  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_11 CTCGCGTGTC  CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT C_sec_12 TAGTATCAGC CCTAYGGGRBGCASCAG GGACTACNNGGGTATCTAAT