title: GSM1254259: HEK293-CT; Homo sapiens; Bisulfite-Seq
library_construction_protocol: High molecular weight genomic DNA was isolated using the conventional proteinase K/organic extraction method. DNA was reduced into 100-300 bp fragments by sonication and treated with DNA-end repair, 3’-dA overhang and ligation of methylated sequencing adapters. Samples underwent bisulfite treatment with the ZYMO EZ DNA Methylation-Gold kit. Desalted, size-selected, PCR amplified fragments were size-selected and qualified libraries were selected for Illumina sequencing according to BGI tech protocol.
attributes.tag: GEO Accession
attributes.value: GSM1254259