title: Human stool deep metagenomic wgs: Sample M8263414
design: Total genomic DNA was extracted using the PowerMag Microbiome DNA isolation kit.  DNA was sheared into fragments of approximately 300-400 bp, which underwent DNA end repair, 3'-adenylation, Illumina adapter ligation, and ligation-mediated PCR.  Individual libraries constructed from each sample were pooled and sequenced on the Illumina HiSeq 2000 platform using the 2x100 bp paired-end protocol.