title: Shotgun sequencing of polyA+ RNA isolated from the H9 cell line
design: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: EXPERIMENT_TYPE
attributes.value: mRNA-Seq
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: RNeasy Blood and Tissue Kit (Qiagen)
attributes.tag: EXTRACTION_PROTOCOL_MRNA_ENRICHMENT
attributes.value: Poly(A)Purist MAG Kit (Ambion)
attributes.tag: EXTRACTION_PROTOCOL_FRAGMENTATION
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: MRNA_PREPARATION_INITIAL_MRNA_QNTY
attributes.value: 20-100 ng
attributes.tag: MRNA_PREPARATION_FRAGMENT_SIZE_RANGE
attributes.value: 200 nt
attributes.tag: RNA_PREPARATION_5'_RNA_ADAPTER_SEQUENCE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: RNA_PREPARATION_3'_RNA_ADAPTER_SEQUENCE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: RNA_PREPARATION_5'_DEPHOSPHORYLATION
attributes.value: NA
attributes.tag: RNA_PREPARATION_5'_PHOSPHORYLATION
attributes.value: NA
attributes.tag: RNA_PREPARATION_3'_RNA ADAPTER_LIGATION_PROTOCOL
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: RNA_PREPARATION_5'_RNA_ADAPTER_LIGATION_PROTOCOL
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_TEMPLATE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_NUMBER_CYCLES
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_PRIMER_CONC
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
attributes.value: SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
attributes.tag: GEO Accession
attributes.value: GSM706044