title: Fragaria nubicola chloroplast, complete genome
design: A modified cetyltrimethylammonium bromide (CTAB) method was used to extract the whole genomic DNA of plant. The concentration of DNA was checked with a Nanodrop 1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). The extracted DNA is sheared to 400600 bp using an ultrasonic technique. The library type was a 500 bp shotgun library according to the manufacturers instructions (NEBNext DNA Library Prep Master Mix Set for Illumina). The library was sequenced by the Hiseq 4000 PE150 platform.