title: GSM3307208: Control (PBS) Treated 3; Oreochromis mossambicus; RNA-Seq
library_construction_protocol: Pituitary rostral pars distalis were 5s pulse sonication on ice Pituitary mRNA (10 μg) was submitted to the North Carolina State University Genomic Sciences Laboratory (Raleigh, NC) for Illumina cDNA library construction. The cDNA was synthesized using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) and tagged with a 5', four-nucleotide barcode. Illumina cDNA libraries (n = 3) were prepared for each treatment (control or leptin).
attributes.tag: GEO Accession
attributes.value: GSM3307208