title: GSM3141913: resistant soybean stem 24 hours post infection biological replicate 1; Glycine max; RNA-Seq
library_construction_protocol: Total RNA was extracted from the stem tissues and fungal material using a Trizol protocol. Samples were cleaned using the RNeasy Plant Mini Kit protocol (RNeasy Plant Mini Kit, Qiagen, Hilden, Germany). RNA concentration and purity were determined by Nanodrop and sample quality was assessed using an Agilent Bioanalyzer 2100 and a RNA 6000 Nano Kit. RNA from each sample was randomly fragmented, and individually indexed libraries were prepared using the TruSeq RNA Sample Preparation v2 kit according to the manufacturer's instructions  protocol. Library was quantified with Qubit HS DNA kit. The quality of the library was checked with an Agilent Bioanalyzer 2100 and an Agilent DNA 1000 kit.
attributes.tag: GEO Accession
attributes.value: GSM3141913