title: GSM3476303: RA1: RA patient1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from synovium tissues were extracted by using Trizol reagent (Invitrogen, USA), the concentration (>= 500ng/ul is qualified), purity (OD260/OD280 range from 1.8 to 2.2) and integrity (28S:18S >=1.5) were detected using Qubit® RNA Assay Kit with Qubit® 2.0 Flurometer (Life Technologies, USA), NanoPhotometer® spectrophotometer (IMPLEN, USA) and RNA Nano 6000 Assay Kit with Bioanalyzer 2100 system (Agilent Technologies, USA), respectively. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3476303