title: GSM3307228: EGFR- cells from F-12266; Homo sapiens; ATAC-seq
library_construction_protocol: EGFR+ and EGFR– cell populations were directly isolated from fresh GM and GBM tissues using FACS, without any prior culturing.  Upon FACS isolation, cells were collected in 1xPBS and tagmented with Tn5 enzyme (Illumina, FC-121-1030) for 50min and consequently frozen until library construction. DNA was amplified with Custom Nextera PCR Primers cointaining barcodes for 9-14 PCR cycles (NEBNext High-Fidelity 2x PCR Master Mix (New England Labs Cat #M0541). Amplicons were run and extracted from agarose gels with a size selection for 150-700bp fragments in order to optimize yield.
attributes.tag: GEO Accession
attributes.value: GSM3307228