title: GSM1262781: Adult Human Islet T2D WFU005; Homo sapiens; RNA-Seq
library_construction_protocol: Small RNA was isolated from the islets by using the mirVana miRNA Isolation Kit (Ambion Cat# AM1560). The glucose experiment and sorted alpha/beta cells were processed with the Illumina truSeq small RNA kit, whereas the whole islet T2D v CTL experiments were processed with the older v1 pre-truSeq kit from Illumina using the long multi-day protocol. Briefly Illumina's 3' adapter then 5' adapter were ligated to small RNA, then amplified using RT-PCR. RT was done with Illumina's RT primer, then PCR with the Primer 1 and Primer 2. Finally libraries were size selected using a gel and aiming for products which correspond to inserts of 22 to 30nt. Consequently, prior to alignment either the truSeq adapter sequence that begins TGGAATTCTCGGGTGCCAAGG or the v1 adapter sequence that begins with TCGTATGCCGTCTTCTGCTTGT need to be trimmed from the reads.
attributes.tag: GEO Accession
attributes.value: GSM1262781