title: GSM2055930: Ath_circRNA; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: The whole plants from different developmental stages (cotyledons emergence, rosette leaves﹥1 mm, rosette growth complete, first flower open, flourishing florescence, first silique shattered, senescence) were collected and then were frozen in liquid nitrogen immediately after harvest and stored at -80°C until use. The total RNAs were extracted from whole plants of each developmental stage using MiniBEST Plant RNA Extraction Kit (Takara, Dalian, Liao-ning, China), according to the manufacturer’s protocol, respectively, and then were mixed equally into an RNA sample. The total RNA sample was treated with Ribo-Zero™ Magnetic Kict and RNase R (Epicentre) to remove the rRNA and digest linear RNA successively. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2055930