title: GSM3834687: Bcell_ATACSeq_WT2_48855; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq was performed as previously described (Buenrostro et al., 2013) with minor modifocations in the tagmentation step where we used a homemade Tn5 enzyme and assembeld the Tn5-adapter tagmentation mix ourselves. However, the adapter sequences and primers used for library amplification are the same as in the Buenrostro et al. protocol. transposed DNA was amplified with RT-qPCR monitoring to avoid oveamplification and the libraries were purified with AMPure XP beads.
attributes.tag: GEO Accession
attributes.value: GSM3834687