title: GSM4217387: Flower buds, RNA-seq_Date1_2; Prunus avium; RNA-Seq
library_construction_protocol: Total RNA was extracted from 50-70 mg of frozen and pulverised flower buds using RNeasy Plant Mini kit (Qiagen) with minor modification: 1.5% PVP-40 was added in the RLT buffer. RNA quality was evaluated using Tapestation 4200 (Agilent Genomics). For RNA-seq libraries, library preparation was performed on 1 μg of high quality RNA (RNA integrity number equivalent superior or equivalent to 8.5) using the TruSeq Stranded mRNA Library Prep Kit High Throughput (Illumina cat. no. RS-122-2103). DNA quality from libraries was evaluated using Tapestation 4200. The libraries were sequenced on a NextSeq500, at the Sainsbury Laboratory Cambridge University (SLCU), using paired-end sequencing of 75 bp in length.
attributes.tag: GEO Accession
attributes.value: GSM4217387