title: bacterial analyze of a high-temperature petroleum reservoir
design: V3 regions of 16S rRNA genes were amplified by primer P2 (5’-ATTACCGCGGCTGCT-3’) and P3 (5’-CCTACGGGAGGCAGCAG-3’), which was tagged with an 8-base (2 protection bases and 6 barcode) to provide primer-specific individual PCRs for different samples. The sequences with no more than one base missing,extra or mismatch between the third and the eighth base of the 8-base barcode, were considered as useful sequences. Extra procedure were needed in actual analysis. The everage length of the useful sequences were 100bp. The barcode of each sample used was shown below.