title: GSM1254495: M.marinum RNA-seq rep1; Mycobacterium marinum; RNA-Seq
library_construction_protocol: RNA-seq: 5 ug of total RNA from each condition was rRNA depleted. Zinc-mediated fragmentation was carried out. cDNA was generated where the second strand was marked with dUTP. Standard Illumina library manipulation was carried out and and Illumina-compatible adapters with in-line indexing were used. Prior to limited amplification, the 2nd uracil marked strand was digested utilising Uracil-DNA Glycosylase enzyme. Sequenced libraries were strand-specific.
attributes.tag: GEO Accession
attributes.value: GSM1254495