title: CD25lo Treg from old C57BL/6 mice
design: CD4+ FoxP3-GFP+ CD25lo Treg were sorted from the spleens of old (18 months) FoxP3-IRES-DTR-GFP C57BL/6 mice. The cDNA library was generated  using the NuGen Ovation RNA-Seq system.