title: expressed sequence tag (EST) from venom gland from Mesobuthus eupeus
design: Briefly,    venom    glands    of M. eupeus were harvested,   homogenized,   and lyzed   by   TRIzol (Life  Technologies).  PolyA(+)  RNA  was  purified on  with  the  mRNA  Purification  Kit  (Amersham Pharmacia   Biotech).   First-strand   and   second-strand  cDNA  synthesis,  linker  addition,  cloning into    pBlueScript    SK+   were    all    carried   out according to the Stratagene cDNA kit. cDNA was purified using a cDNA column (Life Technologies).  Sequencing  was  performed  using the   M13   forward   primer   by   the   ABI   PRISM BigDye   Terminator   Cycle   Sequencing   Ready  reaction  kit  with  AmpliTaq  DNA  polymerase,  FS (Perkin  Elmer)  on  a  Model  373  DNA  Sequencer (Applied Biosystems).