title: Sequencing of Tilapia RAD fragments
design: The RAD library preparation protocol followed closely the methodology originally described in Baird et al. (PloS one, 2008) and comprehensively detailed in Etter et al. (Methods in molecular biology, 2011). The RAD specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in Baxter et al. (PloS one, 2011). The rectriction enzyme SbfI was used.