title: apple branching-control
design: mRNA was isolated from total RNA using magnetic oligo (dT) bends and the recovered mRNA was cleaved into short fragments with the use of a fragmentation buffer. The resulting fragments were used as templates for cDNA synthesis. Following the selection of suitable fragments using AMpure XP beads, purified cDNA was deal with end reparation, the addition of a single adenine nucleotide, and linkage to adapters. The sample libraries were then prepared by polymerase chain reaction (PCR) amplification and the constructed libraries were sequenced on an Illumina HiSeq 4000 system.