title: Transcriptomic analysis of Brugia malayi-elicited alternatively activated macrophages
design: Transcriptomic analysis of Brugia malayi-elicited alternatively activated macrophages
library_construction_protocol: BALBc and IL4Ra-/- mice were intra-peritoneally injected with 700ul thioglycollate (4%)  or intra-peritoneally implanted with 4 B. malayi females and 1 male. Macrophages were harvested 3 days post injection or 21 days post-implantation. Peritoneal exudate cells were harvested by lavage and purified using flow cytometry based upon F4/80 expression. Purity in each sample was confirmed to be >90%. Cells were Qiazol (Qiagen) prior to RNA extraction. Isolation: F4/80+, SiglecF-, B220-, CD4-ve Total RNA was extracted using the miRNeasy kit (Qiagen) according to the manufacturers protocol. Quality was assessed on an Agilent bioanalyzer, all samples had RIN scores >8. Each library was composed of 0.66ug total RNA pooled from 3 mice. RNA-Seq libraries were prepared using the Illumina Paired-End library preparation kit according to the manufacturers instructions.
attributes.tag: Experimental Factor: STRAIN_OR_LINE
attributes.value: BALB/c
attributes.tag: Experimental Factor: TREATMENT
attributes.value: thioglycollate injected