title: Illumina HiSeq 2000 paired end sequencing; RNA-Seq of three Antarctic invertebrates exposed to different rates of warming
design: RNA-Seq of three Antarctic invertebrates exposed to different rates of warming
library_construction_protocol: Tissues were immediately sampled following the thermal treatment and snap frozen in liquid nitrogen. 1°C per 3 days end controls also act as start controls for 1°C per day due to overlap in timing of experiments.1°C per day end controls also act as end controls for 1°C per 3 days due to overlap in timing of experiments. 1°C per hour controls also act as start controls for 1°C per 3 days due to overlap in timing of experiments RNA was extracted using TRI reagent (Bioline, UK) and purified on RNeasy mini columns (Qiagen, Germany) according to manufacturer's instructions Libraries were prepared using Illumina TruSeq RNA Library Prep Kit according to manufacturer's instructions
attributes.tag: Experimental Factor: organism
attributes.value: Paraceradocus miersii
attributes.tag: Experimental Factor: organism part
attributes.value: body wall
attributes.tag: Experimental Factor: environmental stress
attributes.value: 1C increase in temperature per 3 days
attributes.tag: Experimental Factor: cohort
attributes.value: Treated