title: GSM2897956: Adult rep3; Cavia porcellus; RNA-Seq
library_construction_protocol: Hearts isolated from the animals were immediately flushed with excess cardioplegic solution (glucose 227.5mM, mannitol 34.5mM, KCl 30mM, NaHCO3 25mM, pH 7.4) by retrograde perfusion via the aorta to clear all blood. The left ventricular walls were dissected out, frozen in liquid nitrogen and tissues stored until use at -80degC. Total RNA was extracted using RNeasy Fibrous Tissue Mini Kit (Qiagen Ltd., Crawley, UK) according to the manufacturer's instructions. RNA libraries were prepared for sequencing with standard Illumina protocols using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold, Illumina.
attributes.tag: GEO Accession
attributes.value: GSM2897956