title: GSM4550221: Leaf130_col_1 TN_B_028; Acinetobacter sp. Leaf130; RNA-Seq
library_construction_protocol: RNA was isolated from bacterial cells using FastRNA PRO™ BLUE KIT (MP Biomedicals). For plant RNA-seq, RNA was extracted with FastRNA PRO™ KIT with Lysing Matrix E (MP Biomedicals) and DNA was digested with TURBO DNase (Ambion). RNA quality was determined using a 2100 Bioanalyzer (Agilent Technologies, USA). 500 ng total RNA has initially been used for polyA enrichment with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). For bacterial RNA-seq, cDNA libraries were generated with Ovation Complete Prokaryotic RNA-Seq kit 1-8 (NuGEN). For plant RNA-seq, cDNA libraries were generated from polyA enriched RNA using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina
attributes.tag: GEO Accession
attributes.value: GSM4550221