title: 16S-seq of caucasian woman skin
design: Hypervariable regions V3?V4 of the bacterial 16S rRNA were amplified during a first targeted PCR using primers containing Illumina adapters as described in Illumina s 16S Metagenomics Protocol. A second indexing PCR was performed to uniquely barcode each sample with 5  and 3  Nextera XT dual-indices (Illumina). Samples were paired end sequenced (2   251) on the Illumina MiSeq sequencing platform using a MiSeq Reagent Kit v3 (600 cycles) (Illumina) according to a standard protocol.