title: GSM3477874: RNAseq_2_minusOHT_shBRCA1#2_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy mini columns (Qiagen) including on-column DNase I digestion. mRNA was extracted using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB). Library preparation was performed with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina following the instruction manual. Size-selection of libraries was performed using Agencourt AMPure XP Beads (Beckman Coulter) followed by amplification with 12 PCR cycles. Afterwards libraries were quantified and the size was determinded using Experion Automated Electrophoresis System (Bio-Rad).
attributes.tag: GEO Accession
attributes.value: GSM3477874