title: Metogenome Trindade Island
design: After a treatment of the sample, the DNA extraction was made with the Powermax soil DNA isolation kit following the manufacturers instructions. The purity was made using a Nanodrop ND-1000 spectrophotometer. The DNA concentration was determined using a Qubit 2.0 flurometer and dsDNA BR Assay kit (Invitrogen ). The integrity of the DNA was confirmed by electrophoresis in a 0.8 % agarose gel with 1 X TAE buffer. Sequencing was done on the Illumina MiSeq  platform at the High-throughput Genome Analysis Core (HGAC). Fungal ITS1 region was amplified using primers ITS1F (5 -CTTGGCCATTTAGAGGAAGTAA-3 ) and ITS2 (5 -GCTGCGTTCTTCATCGATGC-3 ) using the method described by Smith & Peay (2014). The 16S bioinformatics pipeline was applied, recommended by the Brazilian Microbiome Project available at http://www.brmicrobiome.org/its-profiling-new-illumina (Pylro et al., 2014) that apply Qiime and Usearch 7.0 for filtering, clustering and diversity analysis.