title: TruSeq PCR-free fragment library of Lonchura striata domestica: 220 bp insert
design: Two samples of 2.2 ug of genomic DNA were sonicated using a Covaris M220, 130 μl microTUBE, and presets for a target size of 200 bp (peak incident power 50 W, duty factor 20%, cycles per burst 200, treatment time 160 s). Samples were then purified using Sample Purification Beads (Illumina). Libraries were prepared from this sonicated gDNA using the TruSeq DNA PCR-Free LT Library Preparation Kit (Illumina).  Briefly, samples were end repaired using End Repair Mix 2 then bead purified. Samples were then size selected using a BluePippin 2% agarose, dye-free, external marker gel (Sage Biosciences) set for 190 and 220 bp tight selection. Samples were then a-tailed, adapter ligated, and purified as indicated in the manufacturer's protocol.