title: CCQM MBWG 2013 Study LGC 454 Escherichia coli O157:H7 EDL933 454 sequencing data
design: Three subregions of the 16S rRNA gene were PCR amplified from IRMM-449 Escherichia coli O157:H7 EDL933 reference material and sequenced using the following primer pairs 27F:534R, 357F:926R, and 986F:1492R.  Each amplicon was sequenced in both directions.