title: GSM1261552: DA respiratory tree; Apostichopus japonicus; RNA-Seq
library_construction_protocol: Total RNA from each tissue sample was extracted using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. Recovered Small RNAs were ligated sequentially to 5’ and 3’ adapters. Reverse transcription was performed followed by PCR amplification. The purified PCR products were used directly for cluster generation and sequencing analysis using the Illumina’s Solexa Sequencer according to the manufacturer’s instrunctions
attributes.tag: GEO Accession
attributes.value: GSM1261552