title: Dating the Cryptococcus gattii dispersal to the North American Pacific Northwest
design: Genomic DNA was extracted from the 22 isolates using the ZR Fungal/Bacterial DNA MiniPrep (Zymo Research) kit following manufacturer s instructions. DNA samples were fragmented by sonication and prepared for multiplexed, paired-end sequencing with a 700 base pair insert using Library Hyper Preparation Kit with Standard PCR Library Amplification (KAPA Biosystems) as previously described(30). Libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems) and sequenced to a read length of 300 bp on the MiSeq (Illumina, Inc. San Diego, CA).