title: global transcriptome of Rhizophagus irregularis
design: RNA-seq libraries have been prepared according to Illumina’s protocols using the Illumina TruSeq RNA sample prep kit to analyze mRNA v2 (P.N. RS-122-2001) to analyze mRNA. mRNA were selected using poly-T containing beads to remove other RNA. Then, RNA were fragmented to generate double stranded cDNA to be sequenced. 12 cycles of PCR were applied to amplify libraires, and size selection was performed on E-gel (Invitrogen) . Libraries were quantified by QPCR using the KAPA Library Quantification Kit (PN KK4824) to obtain an accurate quantification. RNA-seq experiments have been performed on an Illumina HiSeq2000, using a paired-end read length of 2x100 pb with the Illumina kits TruSeq SBS sequencing kits v3 (PN FC-401-3001, HiSeq2000). Data have been generated from paired-end libraries (2x100 pb) with insert sizes ranging from 200 to 300 base pairs.