title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of NOMO-1 cell line
design: RNA-seq of NOMO-1 cell line
library_construction_protocol: Cell lines were taken from the stock of the cell lines bank (Leibniz Institute DSMZ â€“ German Collection of Microorganisms and Cell Cultures). Cell lines were authenticated by DNA profiling and cytogenetics. NOMO-1: established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11)(p22;q23) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration. Total RNA was extracted via miRNeasy Mini Kit (Qiagen, Hilden, Germany) including DNase digestion. Production of strand-specific (fr-first strand) mRNA libraries, quality control via Applied Biosystems Fragment fluorometer
attributes.tag: Experimental Factor: replicate
attributes.value: 1