title: RNA-Seq of flt3L treated mus musculus: adult male bone marrow pDC
design: Takara SMART-Seq v4 low input RNA kit  was used to prepare ds cDNA from your samples Illumina compatible sequencing libraries were then prepared using the NEBNext Ultra II FS DNA kit. The libraries were quantified using the Qubit fluorometer and fragment analysis was performed using the Agilent Bioanalyzer. The samples were then further cleaned on the BluePippin to remove any traces of small fragments and they were then run on the Illumina NextSeq in the high output mode at a read length of PE 150bp.