title: Illumina HiSeq 2500 sequencing; Dynamical Modeling of Secondary Metabolism and Metabolic Switches in Streptomyces xiamenensis 318
design: Dynamical Modeling of Secondary Metabolism and Metabolic Switches in Streptomyces xiamenensis 318
library_construction_protocol: For total RNA extraction, cells in the early and late stationary phase at 16h, 24h, 36h and 72h were rapidly cooled using chilled ethanol and harvested by centrifugation (12,000 rpm, 5 min, 4 °C). Total RNA was extracted using RNeasy Mini Kit (Cat#74106, Qiagen) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). The following procedures are mRNA enrichment by rRNA depletion, mRNA fragmentation, first and second cDNA strand synthesis, and library construction.
attributes.tag: Experimental Factor: sampling time point
attributes.value: 36