title: Illumina HiSeq 2000 sequencing; Relief of hypoxia by angiogenesis promotes neural stem cell differentiation by targeting glycolysis
design: Relief of hypoxia by angiogenesis promotes neural stem cell differentiation by targeting glycolysis
library_construction_protocol: CELL CULTURE: Neuro 2a (N2A) cells were maintained in Dulbecco Modified Earl Media / Hams F12 supplemented with 10% fetal bovine serum, 1% non-essential amino acids, 2 mM glutamine, 100 U/ml penicillin and 10 μg/ml streptomycin (all from Life Technologies) and 1.6% NaHCO3 solution (Sigma). N2A cells were transfected with Lipofectamine (Life Technologies). Cells were routinely maintained in culture at 37oC, 5% CO2 and 21% O2.  RNA libraries were created using the Illumina TruSeq RNA sample preparation kit V2 according to the manufacturer’s instructions.
attributes.tag: Experimental Factor: knock out
attributes.value: genetic modification
attributes.tag: Experimental Factor: Gpr124 knock out
attributes.value: genotype