title: gut microbiome Mus musculus: control
design: Amplification by single limited-cycle PCR of the V3 and V4 region 16S r-RNA gene to create a single amplicon of approximately ~460 bp. Primers include Illumina-compatible overhang adapter sequences. Addition of Illumina sequencing adapters and dual?index barcodes to the amplicon target (Nextera XT). Sequencing on MiSeq v2 using paired 250bp reads, and MiSeq v2 reagents (the ends of each read are overlapped generating high?quality, full?length reads of the V3 and V4 region in a single run).