title: RNA-seq of Drosophila melanogaster: pupal antennae of wild type individuals (Canton-S)
design: Antennal A3 segments and aristae were collected from c.a 40 pupae at 52 h APF (after puparium formation), and pooled as a sample. Total RNA was extracted using RNeasy Micro Kit (QIAGEN). A Library for RNA-seq was prepared using  91 ng of total RNA  following the standard protocol of TruSeq Stranded mRNA Sample Prep Kit (Illumina). The cDNA library was amplified by 9 cycles of polymerase chain reaction.