title: GSM2653121: Control rep4; Nicotiana benthamiana; RNA-Seq
library_construction_protocol: Total RNA was extracted from 100 mg of leaf tissue (White and Kaper, 1989). Briefly, the homogenized plant materials were resuspended in 600 μL of extraction buffer (0.1 M glycine-NaOH, pH 9.0, 100 mM NaCl, 10 mM EDTA, 2% SDS, and 1% sodium lauroylsarcosine) and mixed with an equal volume of phenol. The aqueous phase was treated with equal volumes of phenol and chloroform, precipitated with ethanol, and resuspended in sterile water.  Libraries were prepared from total RNA samples with Illumina® Ribo-Zero™ rRNA Removal (Plant Leaf) and ScriptSeq™ v2 RNASeq Library Preparation Kits.
attributes.tag: GEO Accession
attributes.value: GSM2653121