title: GSM4419128: R213G_PBS_2; Mus musculus; miRNA-Seq
library_construction_protocol: Seven days after treatment, mice were euthanized by isoflurane and thoracotomy. Lungs were flushed with 10 mL PBS in the right ventricle, frozen in liquid nitrogen, and stored at -80˚ C. Frozen lungs were pulverized by mortar and pestle. Pulverized lungs were lysed in 2 mL of Qiazol (Qiagen) and homogenized using a Tissue Tearor (Biospec Products). RNA was isolated using the miRNeasy Mini Kit with DNA digestion using the RNA-free DNase kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4419128