title: Whole-genome sequencing of naturally transformed V. cholerae
design: gDNA extracted from cultures grown overnight in LB medium. 2ml of cells culture were used after centrifugation. Genomic DNA kit (Qiagen) using genomic-tip of 100/G used for extraction. After gDNA precipitation, the samples extracted for further Illumina sequencing were directly transferred in 10 mM Tris-HCl buffer at pH 8.0.