title: GSM3307332: miRWT1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA for sorted TAMs isolated form LLC tumors implated in WT or mir-21 null mice was extracted using Rneasy Micro Kit (cat no 74004, Qiagen, Venlo, Netherlands). Briefly: sorted TAMs were resuspended in the lysis buffer provided by the kit and following ethanol washes and genomic DNA digestion RNA was eluted. The Kapa mRNA Hyper prep kit (Cat# KK8581) was used. The Kapa DI Adapter Kit (Cat# KK8722) was used for the indexes.
attributes.tag: GEO Accession
attributes.value: GSM3307332