title: UluPandan February time point B8 RNA Illumina HiSeq 2000 runs
design: Extracted total RNA was subjected to DNase digestion (Ambion Turbo DNase) to remove trace amounts of DNA prior to RNA library preparation. The DNA-depleted total RNA samples from the October and February samples were subjected to TruSeq RNA Sample Preparation (Illumina), following the manufacturer's protocol with the following modification: The initial mRNA purification step was skipped and instead, 120 ng of total RNA was reduced to a volume of 5 **L, using a MinElute PCR Purification column (Qiagen). Library preparation with the concentrated total RNA was started at the RNA fragmentation step in the manufacturer's protocol.