title: GSM1689104: N29c; Homo sapiens; Bisulfite-Seq
library_construction_protocol: DNA was isolated using a DNA Blood mini kit (Qiagen) following the manufacturer’s protocols. Genomic DNA was sonicated to 200-300 bp using the Covaris S2 system and then the fragmented DNA was end-repaired using a combination of T4 Polynucleotide Kinase and Klenow polymerase .The blunt, phosphorylated ends were treated with Klenow fragment (3’-5’ exo-) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's methylated adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation , the DNA was bisulphite treated using the EZ DNA Methylation-Gold Kit (ZYMO),and then PCR amplified using JumpStart Taq DNA Polymerase (Sigama)
attributes.tag: GEO Accession
attributes.value: GSM1689104