title: Illumina HiSeq 2000 paired end sequencing; Expression in hypothalamus of wild-derived mice M.m. domesticus
design: Expression in hypothalamus of wild-derived mice M.m. domesticus
library_construction_protocol: Mice were sacrificed at the age of 11 to 13 weeks. All dissections were done by the same person following personal instructions and standardized protocols designed by Valery Grinevich (MPI for Medical Research Heidelberg for HYP). Brains were removed and further dissection was performed on wet ice using gross anatomical borders (i.e., optic chiasm arms, optic tracts, and mammillary body) as markers. Prepared tissues were immediately frozen on dry ice and kept at -70 degree centigrade until RNA preparation. Using TRIzol Reagent with the PureLink RNA Mini Kit, invitrogen/ambion With On-Column PureLink DNase Treatment during Purification Illumina TruSeq RNA Sample Preparation Kit v2
attributes.tag: Experimental Factor: strain
attributes.value: Koeln
attributes.tag: LIBRARY_STRAND
attributes.value: not applicable