title: Illumina paired end whole exome sequencing of hairy cell leukemia sample 3
design: Illumina paired-end sequencing of sample SAMPID random 300 bp insert library. Genomic DNA was randomly fragmented using a Covaris S2 Focused-ultrasonicator to a mean size of 300bp.  Fragment ends were repaired and phosphorylated with T4 DNA Polymerase, Klenow-fragment, and T4 Polynucleotide Kinase.  A 3'-A overhang was introduced with Klenow-exo-minus followed by ligation to Illumina paired-end adaptors.  Ligation products with mean size 240bp +/- 20% were isolated on a Caliper LabChIP XT followed by amplification using Illumina PCR primers.  Pooled, indexed libraries were captured using the Agilent SureSelect Human All Exon 50Mb kit.