title: GSM1254718: HetrBC2-Chromatin pull down; Nostoc sp. PCC 7120; OTHER
library_construction_protocol: Cells were cross linked with disuccinimidyl glutarate (DSG) and ethylene glycol bis(succinimidyl succinate) (EGS), followed by formaldehyde. Cells were broken by bead beating and DNA was sheared via sonication. His-tagged HetR was bound to Dynabeads, crosslinks were reversed, proteins were digested, DNA was column purified. DNA was prepared for sequencing with the Illumina Chromatin pull down Sample prep kit by the Next Generation Sequencing Core at The Scripps Research Institute (La Jolla, CA) following the manufacturer’s protocol. Sequencing was performed on the Illumina HiSeq platform with 4 samples multiplexed on one cell, yielding approximately 40 million 40-bp reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM1254718