title: GSM2994984: H25; Mycobacterium tuberculosis H37Rv; RNA-Seq
library_construction_protocol: total RNAs extracted using the Direct-zol  RNA MiniPrep kit (Zymo Research #R2052) according to the manufacturer's instructions. 2 µg of total RNAs were subjected to rRNA  depletion using the Ribo-Zero rRNA Removal kit (Illumina #MRZB12424). The reaction clean-up was performed using the RNeasy Mini kit (Qiagen #74104). The cDNA library was prepared using the NEBNext^® Ultra™ Directional RNA Library Prep kit for Illumina^® (New England Biolabs #E7420S), NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (New England Biolabs #E7335S)*, *NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2) (New England Biolabs #E7500S) according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2994984