title: mRNA extracted from Chunee stalk shoots
design: Stalks of SP70-1143 and Chunee varieties, kindly provided by Embrapa Agrobiology (Serop*dica, RJ), were germinated in a mixture of sand and vermiculite in the ratio of 2 to 1 respectively. Watering was done 2 times per week and the temperature maintained between 25*C and 30*C with an irradiance of 60μmol photons m-2 s-1 for 12h d-1.  The tissue samples were collected 15 days after planting. For RNAseq libraries construction, samples collected from fifteen plantlets were pooled and total RNA was extracted from frozen material according to Logeman et al. (1987). The quality and quantification of RNA samples was checked via agarose gel and Agilent 2100 Bioanalyzer TM (Agilent Technologies, Aplo Alto, CA). Two biological replicates of each plant material were used for RNAseq Illumina sequencing at FASTERIS (Switzerland).