title: Opposite wood xylem transcriptome from Pinus radiata
library_construction_protocol: Total RNA was extracted from tissue using a modified CTAB method with removal of genomic DNA contamination via DNaseI and agarose gel electrophoresis. RNA integrity and successful removal of DNA contaminants was confirmed using a NanoDrop 1000 spectrophotometer (Thermo Scientific). RNA libraries were prepared for sequencing using the Illumina TrueSeq RNA sample preparation kit. Libraries were pooled together for sequencing on an Illumina HiSeq 2000.