title: GSM1261896: 5ais; Homo sapiens; RNA-Seq
library_construction_protocol: 4-micron thickness sections on PEN membrane slides were prepared under RNAase free conditions.  Laser capture microdissection of distinct areas of normal lung, pure BAC and invasive adenocarcinoma was performed using the Applied Biosystems Arcturus Veritas Microdissection System (Life Technologies, Grand Island, NY). Total RNA was isolated using the Ambion RecoverAll kit (Life Technologies, Grand Island, NY) according to manufacturer’s protocol. Sequencing libraries were prepared using the Ovation RNA-Seq FFPE (7150-08) and Encore NGS Library System 1 kits (300-08; sans multiplexing) according to manufacturer’s protocol (NuGEN Technologies, Inc., San Carlos, CA). Non-multiplexed sequencing of 100 bp paired-end reads was performed using the Illumina GAIIx (Illumina, Inc., San Diego, CA) according to manufacturer’s protocols
attributes.tag: GEO Accession
attributes.value: GSM1261896