title: GSM1257852: BP-55 CTC; Mus musculus; RNA-Seq
library_construction_protocol: Blood samples were collected from mice with high tumor burden following tamoxifen injection. Blood from each mouse was split and processed through the CTC-chips functionalized with anti-CSPG4/MCAM antibody and control IgGs, respectively. Matched primary (from the tamoxifen injection site) and metastatic (from upper or lower back) tumors were harvested from the same mouse and immediately flash-frozen in liquid nitrogen. RNA extraction and single molecular sequencing was described previously in PMID: 22763454) Qiagen RNeasy MinElute kit followed by Helicos single molecule sequencing: Pubmed ID 18388294, 20133332
attributes.tag: GEO Accession
attributes.value: GSM1257852