title: Illumina HiSeq 4000 sequencing; RNA sequencing in islets from Rip-Cre CDK8fl/fl and CDK8fl/fl mice with or without metabolic stress.
design: RNA sequencing in islets from Rip-Cre CDK8fl/fl and CDK8fl/fl mice with or without metabolic stress.
library_construction_protocol: RNA was extracted using PicoPure columns with on-column DNase treatment. Quality was verified on Bioanalyzer Islets were isolated from mice (with or without STZ) and picked manually before being frozen down as a pellet TruSeq Stranded mRNA Library Prep Kit
attributes.tag: Experimental Factor: age
attributes.value: 9
attributes.tag: Experimental Factor: genotype
attributes.value: CDK8fl/fl
attributes.tag: Experimental Factor: stimulus
attributes.value: streptozotocin