title: WGS water-column Oct,2013 (after rain): replicate 2
design: Water was collected from near the surface (0-1m depth) from North Shore Channel, and passed on-site in succession through ~1.6 um pore size glass fiber filter for pre-filtration and captured on a 0.22 um pore size polycarbonate membrane filter for collecting free-living microbial component. DNA was extracted using phenol:chloroform based extraction, and submitted for WGS sequencing with paired-end format (100bp each) and approximate insert size of 500bp. Water samples were collected in duplicates, and this library represents one of the biological replicates.