title: Transcriptome analysis of silkworm, Bombyx mori, during early response to Beauveria bassiana challenges
design: The third instar larvae were treated with Beauveria bassiana solution, then RNA from the whole body of the larvae were isolated.The 5' sticky ends of the cDNA fragments were generated by endonuclease NlaIII. Then the cDNA fragments are purified by magnetic beads and the Illumina adaptor 1 is ligated to the 5' end of the cDNA fragments. Another endonuclease, MmeI, is used to digest the cDNA fragments, cutting at 17bp downstream of the CATG site to produce the tags with adaptor 1. After removing 3' fragments with magnetic beads precipitation, Illumina adaptor 2 is ligated to the 3' ends of tags, then tags with different adaptors are acquired and a 21bp tag library is constructed.