title: Mimulus vernalizaton and photoperiod LMC24
design: 2 x 100bp paired-end using TruSeq RNA Library Preparation Kit v2 (Illumina, CA, USA). We randomly assigned 200 newly germinated seedlings of each inbred line to one of two plant growth chambers for 2.5 wks: long-day warm (16 hr daylength, constant 21°C temperature) or short-day warm (8 hr daylength, constant 21°C temperature). After 2.5 wks, we moved all plants to a common short-day cold (8 hr daylength, constant 4°C temperature) chamber for six weeks of vernalization. Following vernalization, we reassigned plants to a long-day warm (16 hr daylength, constant 21°C temperature) chamber until flowering.