title: GSM3476824: pcDNA2_S2_R1_001; Homo sapiens; RNA-Seq
library_construction_protocol: After 24 h incubation at 37°C, RNA was extracted using the miRneasy kit (QIAGEN) for RNA sequencing. Extracted RNA samples were examined with Bioanalyzer for RNA integrity and concentration before sequencing. RNA integrity number was 9.62 with 0.98 standard deviation. 100 ng of total RNA was used to generate RNA-Seq libraries using Illumina TruSeq stranded mRNA LT kit (Cat# RS-122-2101).   Libraries prepared with unique barcodes were pooled at equal molar ratios. The pool was denatured and sequenced on Illumina NextSeq 500 sequencer using high output V2 reagents and NextSeq Control Software v1.4 to generate 75 bp single reads, following manufactures protocol (Cat# 15048776 Rev.E).
attributes.tag: GEO Accession
attributes.value: GSM3476824