title: Target amplicon sequencing of mouse Tyr gene: indel analysis of embryo explants injected with CRISPR-Cas9
design: Primer pairs used for PCR were uniquely indexed for each embryo at the 5  ends with 16-nucleotide asymmetric barcodes. PCR products were pooled for SMRTbell template preparation and sequenced using a PacBio RS II sequencer following standard guidelines and procedures by the University of Massachusetts Medical School, Deep Sequencing Core.