title: Olive fruit transcriptome
design: Total RNA was isolated from each sampling point separately using the Spectrum Plant Total RNA Extraction Kit (Sigma-Aldrich) following the manufacturer’s protocols. RNA quality and integrity was regularly inspected by formaldehyde agarose gel electrophoresis. The concentration of RNA samples and A260/A280 ratios were measured by Nano Drop2000c Spectrophotometer (Thermo Scientific). At the end equimolar RNA quantities of all samples were mixed together to obtain the representative sample of all RNA expressed throughout the developmental and ripening period of olive fruits.  Pooled sample of RNA was reverse transcribed and normalized using normalization service (Evrogen Lab, Russia), In short, the cDNA was processed using SMART approach (Zhu, et al., 2001) and normalized by highly efficient method employing Red King (Kamchatka) crab duplex specific nucleases (Zhulidov et al., 2005).  GsuI treated, normalized cDNA samples were further processed in concatemers, sheared and sequenced (half of the region of the picotiter plate) using next-generation sequencing service provider (GATC Biotech, Konstanz, Germany).