title: GSM2897965: baba1; Solanum lycopersicum; RNA-Seq
library_construction_protocol: TRIzol according to the manufacturer instructions. Ethanol precipitated RNA PolyA+ RNAs were selected from 6µg total RNA with Dynabeads mRNA purificationkit (invitrogen). RNA were  fragmented 10 min at 95°C in RNAseIII buffer (Invitrogen) then adapter-ligated, reverse transcribed and amplified (5 cycles) with the reagents from the NEBNext Small RNA Library Prep Set for SOLiD. Amplified libraries were purified on Purelink PCR micro kit (Invitrogen) then subjected to additional PCR rounds (7 cycles) with primers from the 5500 W Conversion Primers Kit (Life Technologies). After Agencourt® AMPure® XP beads purification (Beckman Coulter), libraries were size-selected from 200 nt to 300 nt with the LabChip XT DNA 300 Assay Kit (Caliper Lifesciences). Libraries were finally converted with the enzyme kit from the 5500 W Conversion Primers Kit (Life Technologies), quantified with the Bioanalyzer High Sensitivity DNA Kit (Agilent) and sequenced on SOLiD 5500XL (Life Technologies) with single-end 50b reads.
attributes.tag: GEO Accession
attributes.value: GSM2897965