title: Illumina HiSeq 2000 paired end sequencing; RNA-seq of Leishmania mexicana promastigotes, axenic amastigotes and intracellular amastigotes
design: RNA-seq of Leishmania mexicana promastigotes, axenic amastigotes and intracellular amastigotes
library_construction_protocol: Axenic amastigotes were generated by dilution of stationary phase Leishmania mexicana promastigotes (WHO strain MNYC/BZ/62/M379) into Schneider’s Drosophila medium at pH 5.5 to a density of 3x10E6 cells/ml and incubation at 34°C for 24h prior to RNA extraction. For mapping of splice acceptor sites and determination of transcript abundances, polyadenylated RNA was purified from total RNA, converted to cDNA using random hexamer primers, sheared and size selected for fragments ~200bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2.
attributes.tag: Experimental Factor: organism
attributes.value: Leishmania mexicana
attributes.tag: Experimental Factor: developmental stage
attributes.value: axenic amastigote
attributes.tag: Experimental Factor: host
attributes.value: not applicable (outside host)