title: GSM3145527: Meng3/WT/TRAV11; Mus musculus; OTHER
library_construction_protocol: preselection DP cells were sorted from WT and SKG thymi, and RNA from DP cells were extracted total RNA from DP cells were reverse transcribed to generate cDNA, which was used as template to do the PCR. PCR was performed for amplification of the Trav9, Trav11 and Trav19 gene segments with specific forward primers for each Va region each with an Illumina adaptor sequence on the 5' end and and a reverse primer for the α-chain constant region. PCR products were purified and sequenced on a Miseq system with a MiSeq Reagent Kit v2-500 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3145527