title: GSM1933934: Retroviral cDNA Library (Genomic DNA from Bone Marrow Cells); Homo sapiens; OTHER
library_construction_protocol: cDNAs were amplified by PCR from the plasmid mix or from the genomic DNA from the bone marrow cells transduced with the retroviral cDNA library. The amplified DNA were extracted with PCR clean up kit from Clontech Co. The sequencing libraries were then prepared according to the manufacturer’s instructions of TruSeq Nano DNA Library Prep Kit (Illumina, #FC-121-4003). Briefly, fragmentation of 1ug of genomic DNA was performed using adaptive focused acoustic technology.(AFA; Covaris) The fragmented DNA is repaired, an ‘A’ is ligated to the 3′ end, Truseq adapters are then ligated to the fragments. Once ligation had been assessed, the adapter ligated product is PCR amplified. The final purified product is then quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using the Agilent Technologies 2200 TapeStation. (Agilent technologies). And then we sequenced using the HiSeq™ 2500 platform (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM1933934