title: Growing-4
design: Sampling was conducted via esophageal tubing and snap-frozen with liquid nitrogen. Total DNA was extracted from rumen samples with the PowerMax Soil DNA Isolation Kit (MO BIO Laboratories, Inc.) according to the manufacturers protocols. Metagenomes were prepared with the Nextera XT DNA Library Prep Kit (New England Biolabs) and sequenced on the Illumina HiSeq platform using 150 bp paired-end sequencing.