title: Illumina HiSeq 2000 paired end sequencing; Tuning the transcriptional response to hypoxia through HIF prolyl- and asparaginyl-hydroxylase inhibition:Sequencing Data
design: Tuning the transcriptional response to hypoxia through HIF prolyl- and asparaginyl-hydroxylase inhibition:Sequencing Data
library_construction_protocol: Human cell lines (MCF-7) were cultured in DMEM (D6546-500ML; Sigma Aldrich) each supplemented with 10% fetal bovine serum (F7524-500ML; Sigma Aldrich), 2 mM L-glutamine (G7513- 100ML; Sigma Aldrich), 50 units/ml of penicillin, and 50 μg/ml of streptomycin (P0781-100ML; Sigma Aldrich). 0.5% oxygen for 16 hours total RNA was prepared using mirVanaTM miRNA isolation kit (AM1560; Life Technologies), according to manufacturer’s protocol. Genomic DNA was removed from RNA samples using TURBO DNA-freeTM Kit (AM1907; Life Technologies) according to the manufacturer’s protocol. poly-A selection from total RNA
attributes.tag: Experimental Factor: 0.5% oxygen
attributes.value: treatment