title: Illumina HiSeq 1500 paired end sequencing; RNA-seq of mouse alveolar macrophages (ADAR1 deficient and WT)
design: RNA-seq of mouse alveolar macrophages (ADAR1 deficient and WT)
library_construction_protocol: Alveolar macrophages of CD11c-Cre-ADAR1Δ7-9/Δ7-9 and CD11c-Cre-ADAR1wt/wt control mice were collected from BAL fluid and purified by CD11b MagniSort depletion and CD11c MagniSort enrichment.  Whole RNA was isolated using the RNeasy Plus Micro Kit and stored at -80°C until further processing. Integrity of total RNA was assessed on the Bio-Rad Experion. Semi-automated library preparation was performed on the IP-Star (Diagenode) using the TruSeq Stranded mRNA Kit (Illumina). Each library was quantified and qualified on a Bioanalyzer 2100 (High Sensitivity DNA Kit, Agilent Technologies). Equimolar amounts of libraries were pooled and quantified by digital PCR using the QuantStudio™ 3D Digital PCR System (Life Technologies).
attributes.tag: Experimental Factor: phenotype
attributes.value: ADAR1 knockout