title: RNASeq of Micropterus salmoides: Low EE2 exposed spleen 3
design: Largemouth bass were euthanized in MS-222, and liver and spleen were immediately removed and flash frozen in liquid nitrogen. RNA was extracted from each tissue using a Qiagen RNEasy column kit. Paired-end sequencing libraries were prepared using an Illumina TruSeq Stranded kit following manufacturer's protocols. Libraries were sequenced as 75 bp paired-end reads in 2 lanes on an Illumina NextSeq500.