title: RNA-seq of Pangasianodon hypophthalmus: 4-cell stage
design: Total RNA was extracted from the embryonic  4-cell stage of striped catfish using Trizol reagent (Invitrogen). Then, cDNA library was constructed using TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the manufacturer s procedure. The cDNA library was sequenced using Illumina Hiseq 2500 sequencing platform.