title: RNA-Seq of Vesicular Stomatitis from 2019 Southwest United States
design: First-strand synthesis was performed using SuperScript III reverse transcriptase (Invitrogen) with random primers. Second strand synthesis was performed using Sequenase enzyme (Affymetrix) and then amplified with Taq polymerase (Clontech). Sequencing libraries were constructed using the Nextera XT kit (Illumina) and sequenced with a 500-cycle MiSeq sequencing kit (v2)