title: RNA-Seq of T7 RNAP reactions seeded with a predefined DNA pool that had been DNase treated (Experiment/Result: RNAs replicated by T7 RNAP can originate through partial instruction from DNA seeds), Reaction in tube format
design: library_source= in vitro RNA population; For RNA-seq library preparation, there are four main molecular biology steps: 1) 3 ligation of ssDNA adapter to RNA, 2) Reverse transcription (using a primer that is complementary to the ssDNA adapter used in step 1), 3) Circularization of cDNA and 4) PCR. For each sample we sequenced, we used gel electrophoresis to visualize and extract intermediate products during library preparation following each of the steps of 3 ligation, reverse transcription and PCR; fastq data uploaded corresponds to raw data after demultiplexing (adapters not trimmed in uploaded data); further details on library preparation, data processing, size selection of RNAs for sequencing, and adapter sequences including molecular barcode sequences and positions,  can be found in the associated manuscript; this sample was initially sequenced as part of MiSeq run AF_SOL_895 and subsequently re-sequenced as part of MiSeq runs AF_SOL_897, AF_SOL_898, AF_SOL_899, AF_SOL_903, AF_SOL_904 and AF_SOL_905 for greater sequencing depth--the uploaded data consists of a concatenation of reads from all the MiSeq runs