title: GSM3984847: Whib6-3xfl (DespM) Rep 2, part 2; Mycobacterium marinum; RNA-Seq
library_construction_protocol: The cultures were collected by centrifugation and stored at -80 for 24 hours. Cells were lysed using a bead beater. Total RNA was prepared from the pellets using the Qiagen RNeasy kit, as described by Bosserman et al., PNAS, 2017. cDNA libraries were constructed using the Illumina TruSeq RNA library preparation kit (v2), omitting the polyA selection step. Multiplexing sequences are included in the title of the associated fastq.gz files. After library quality control, sample libraries were pooled and sequenced in two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) in 50bp, single-end read format (SE50). After the sequencing run, reads were de-multiplexed and converted to FASTQ format using the Illumina bcl2fastq (v1.8.4) script.
attributes.tag: GEO Accession
attributes.value: GSM3984847