title: 2x151 bp RNA-Seq Metatranscriptome for J2-801-BM1-B2 hydrothermal vent Fe mat
design: In situ fixed RNA expression using RNALater. pTXB1 internal standard added prior to library prep. RNA-seq library prepared from total RNA using the Ovation RNA-seq system V2 kit, which included preferential priming of non-rRNA sequences. This is a test run of the efficiency of the ribosomal reduction on the MiSeq. Paired end 2x151 bp run.