title: Shotgun sequencing of 10 week old wild-type mus musculus: fecal samples
design: Libraries were prepared according to the manufacturers protocol using Nextera XT Library Preparation Kit (Illumina #FC-131-1096). Nextera XT libraries were pooled at equimolar amounts of 2nM per library to create a sequencing pool. Library QC is performed using the Agilent D1000 HS tapes (#5067-5582) on the TapeStation 4200 (Agilent # G2991AA) as per the manufacturers protocol. The library was prepared for sequencing on the NextSeq500 (Illumina) using NextSeq 500/550 High Output v2 2 x 150bp paired end chemistry. The reads were mapped against mm10 to remove host-contaminated reads.