title: GSM3983276: HBL-100 small RNA; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from cells using Trizol reagent (Life Technologies, USA). We do the following treatments before library preparation for total RNA samples: 3'-aminoacyl (charged) deacylation to 3'-OH for 3' adaptor ligation, 3'-cP (2',3'-cyclic phosphate) removal to 3'-OH for 3' adaptor ligation, 5'-OH (hydroxyl group) phosphorylation to 5'-P for 5'-adaptor ligation, m1A and m3C demethylation for efficient reverse transcription.  Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter. The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100. RNA libraries were prepared for sequencing using standard Illumina protocols