title: Illumina Miseq 18S Fungal birch rhizosphere communities on Ni-Cu tailings: Sample H20Fun
design: DNA was extracted from samples using the PowerSoil® DNA Isolation kit (MO BIO Laboratories Inc., Carlsbad, CA). A 10 minute homogenization step was performed using a vortex adaptor. Samples were frozen and stored for analysis at a later date. Illumina Miseq was completed on samples collected in 2014. Samples were processed by metagenombio(Waterloo, Canada). Sequencing was done with general forward (CCTACGGGAGGCAGCAG) and reverse (GGACTACHVGGGTWTCTAAT) bacterial 16S primers.