title: GSM4490666: DIE (scRNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were eluted and resuspended in PBS to reach a final concentration of 1000 cells /µl. 17000 cells were processed using the Single Cell 3' Reagent Kits V2 from 10x Genomics according to the manufacturer instructions. After GEM generation and barcoding, post GEM-RT cleanup, cDNA amplification and library construction, cDNA was diluted to 5nM in EB buffer. Illumina sequencing libraries were sequenced on one lane with a HiSeq 4000 system (100bp sequencing, 50k row reads per cell) after quality assessment with the Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM4490666