title: Metabarcoding of sediment samples: 18S V9 marker
design: Amplicons were generated by PCR using the ribosomal 18S V9 marker. This was done in triplicate and pooled. The libraries preparation was performed using Illumina TruSeq DNA PCR-Free Library Preparation Kit. The libraries were quantified with qPCR using KAPA Library Quantification Kit and sequenced on a MiSeq instrument (Illumina) using paired-end sequencing for 600 cycles with kit v3.