title: High-quality resolution of outbreak-related Zikavirus genome and discovery of new viruses using IonTorrent-based metatranscriptomics
design: Here, we applied a metatranscriptomics approach optimized for Ion Torrent-derived data inorder to reconstitute the whole genome of a Zika virus strain associated to the Brazilian outbreak in2015 (GenBank KR816334); only a short fragment of this genome (310 nucleotides) had previouslybeen described by Soares et al 2015. The library was prepared from Aedes albopictus C6/36 cell lineinoculated with ZIKV supernatant using Ion Total RNA-seq kit v2 according to manufacturersprotocols. Sequencing was performed using