title: 454 GS FLX Titanium sequencing; qiita_ptid_5934:11920.SC2
design: Holothuria_glaberrima_intestinal_microbiota
library_construction_protocol: 454 sequence of V4 region of 16SrRNA
attributes.tag: adapter
attributes.value: GCCTCCCTCGCGCCATCAG
attributes.tag: barcode
attributes.value: CAGATCGGATCG
attributes.tag: center_name
attributes.value: UPPRP
attributes.tag: center_project_name
attributes.value: Holothuria_glaberrima_intestinal_microbiota
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GAGTGCCAGCMGCCGCGGTAA ; REV:CCGGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GAGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: UPR-SGF
attributes.tag: run_date
attributes.value: Not provided
attributes.tag: run_prefix
attributes.value: H5HHLPV02
attributes.tag: runid
attributes.value: SC
attributes.tag: sequencing_meth
attributes.value: pyrosequencing
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4-V5