title: GSM4059876: ko_5wk_327; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized and perfused with PBS containing 5U/mL heparin. The brain was harvested and coronal slices were cut using an adult mouse matrix (69080-C, Electron Microscopy Sciences). Coronal slices (cut to include bregma -0.88 to -1.855) were prepared from untreated animals (3mm thick) and cuprizone treated animals (2 mm) to normalize total RNA quantity. The corpus callosum was then micro-dissected, flash-frozen in liquid nitrogen, and RNA was extracted using the Isolate II Kit (Bioline). RNA-seq (Illumina) was performed by the Genomic Sciences Lab or the Genome Analysis and Technology Core at the University of Virginia. RNA was extracted using the Isolate II Kit (Bioline). Isolated mRNA was subject to reverse transcriptase to cDNA, 150bp read lengths and paired-end sequencing
attributes.tag: GEO Accession
attributes.value: GSM4059876