title: Drosophila  neotestacea RNA-sequencing: Sneg_HApos
design: Drosophila neotestacea with and without Spiroplasma infection, with and without Howardula aoronymphium exposure. H. aoronymphium exposure was done during larval stage, and samples collected at 5-day intervals (larvae, pupae, adults) and pooled for library construction (Qiagen RNEasy kit extraction, polyA enrichment).  4-libraries (Illumina 75bp PE) were constructed and sequenced in a factorial design with all infection combinations.