title: GSM1932132: Original sgRNA replicate 1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from the sorted cells using QIAamp DNA blood mini kit (QIAGEN) The library construction follows Illumina’s TruSeq method. Briefly, sgRNA sequences were amplified by 2 rounds PCR with the first pair of primers, ACACTCTTTCCCTACACGACGCTCTTCCGATCT TCTACCTGCTCAACCTGGCC (forward) and GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC AAGTCCCACTGGGCGGC (reverse), followed by amplification with the second pair of primers, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA (forward) and CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTG(reverse, where NNNNNNN indicates index sequences). TruSeq
attributes.tag: GEO Accession
attributes.value: GSM1932132