title: RNA-seq of Brugia malayi L3 larvae Rep a
design: Total RNA was extracted by organic extraction using Trizol and the PureLink RNA mini kit (ThermoFisher). Ribosomal RNA (rRNA) depletion was performed using Terminator (Epicentre), a 5-phosphate-dependent exonuclease that degrades transcripts with a 5 monophosphate. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer instructions. Library quality was assessed using a D1000 ScreenTape Assay (Agilent) prior to sequencing. Library concentrations were assessed using the qPCR library quantification protocol (KAPA biosystems). Libraries were sequenced on the Illumina NextSeq500 platform with 150bp paired-end reads.