title: GSM2477202: HCCLM3TimeZero; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA were extracted using E.Z.N.A.® HP Tissue DNA Maxi Kit (OMEGA bio-tek, USA). The integrated gRNA region was PCR amplified using Q5® High-Fidelity DNA Polymerase (NEB, USA) with V2 adaptor primers. Illumina compatible libraries were generated using F8N forward primer and sample specific barcoded reverse primers.
attributes.tag: GEO Accession
attributes.value: GSM2477202