title: 3152 Day 0  Female Temperamental
design: Fecal samples were collected from cattle at weaning and 4 days after weaning. Bacterial DNA was extracted using the PowerFecal kit (MoBio, Carlsbad, CA). Illumina sequencing of the V4-V6 region (E. coli position 530-1100, 530F GTGCCAGCMGCNGCGG, 1100R GGGTTNCGNTCGTTR) of the 16S rRNA was performed on a MiSeq Instrument.