title: GSM2396954: CD3_repB; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted following previously published gating strategies, shown in Figure S1 and RNA was extracted using NucleoSpin RNA XS (Machery Nagel), including DNase treatment. Between 3000 and 10,000 cells were used as library input using Clontech’s SMARTer Ultra Low input RNA Sequencing Kit (Cat. No. 634823). cDNA was sheared to 300 bp on a Covaris LE220 and used as input for Clontech’s low input library preparation for Illumina Sequencing kit (Cat. no. 634947). Libraries were sequenced paired-end 100 bp on an Illumina HiSeq2500.
attributes.tag: GEO Accession
attributes.value: GSM2396954