title: GSM3984240: Dox3; Mus musculus; RNA-Seq
library_construction_protocol: GFP-positive cells were sorted directly into Trizol LS (Invitrogen). Completely lysed samples were treated with chloroform to separate phases. The aqueous phase containing RNA was transferred and purified using a RNeasy Micro Kit (Qiagen). On-Column DNase I digestion was conducted to remove genomic DNA contamination. The quality of RNA from sorted cells was verified by Agilent 2100 Bioanalyzer. Three individual RNA samples for each group with high quality (RIN > 7) were enrolled for library preparation. mRNA amplification was performed using 5ng total RNA with a Clontech Smarter RNA seq v4 kit. RNA-seq libraries were prepared using Illumina Nextera library preparation kit.
attributes.tag: GEO Accession
attributes.value: GSM3984240