title: GSM4121292: MkMP_smallRNA_rep2; Homo sapiens; ncRNA-Seq
library_construction_protocol: isolation protocol: Blood for isolation of human platelets (PLTs) was collected by venipuncture from adult healthy human volunteers, into a syringe with ACD buffer at a volume ratio of 1:6 (ACD:blood). Blood was centrifuged at 250 × g for 10 min and the platelet-rich plasma was isolated from the supernatant. PLTs were then pelleted at 750 × g for 10 min, followed by 1 wash with HEN buffer (10 mM HEPES, pH 6.5, 1mM EDTA, 150 mM NaCl) containing 0.05 U/ml apyrase. After that, PLTs were resuspended in HEPES-Tyrode's buffer (137 mM NaCl, 20 mM HEPES, 5.6 mM glucose, 1 g/l BSA, 1 mM MgCl2, 2.7 mM KCl, 3.3 mM NaH2PO4). Total RNA was isolated using the miRNeasy micro kit (Qiagen). RNA concentration was measured by NanoDrop and size distribution of total RNA was analyzed using an ABI Prism 3130XL Genetic Analyzer. Small-size RNA (18-40 nt and 40-150 nt in size) was purified by 15% polyacrylaminde/urea gels and eluted from gels for library construction using Illumina TruSeq Small RNA Sample Prep kit according to the manufacturer's protocol. The 11 libraries described above were pooled together. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4121292