title: DNA-Seq of Staphylococcus aureus USA 100:  Oxford Nanopore
design: Nanopore sequencing libraries were generated from genomic DNA using the Oxford Nanopore Rapid Barcoding Sequencing SQK-RBK004, according to the manufacturers instructions. (Oxford Nanopore Technologies) Briefly 400ng of gDNA was fragmented and tagged with a barcode specific adapter. Resulting tagged fragments were ligated with Nanopore specific sequencing adapters and loaded onto a FLO-MIN106 (R9 Version) SpotON flow.  Sequencing was carried out for 48 hours with live basecalling enabled.