title: GSM1936765: ABA 3h rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted from Arabidopsis seedlings using TRIzol reagent (Ambion), treated with TURBO DNase (Ambion) and purified using RNeasy mini spin column (Qiagen). The quality of purified RNA was assessed using an Agilent 2100 Bioanalyzer. The cDNA libraries for ssRNA-seq were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation Kit according to the low sample protocol guidelines. The quality and size of each sample library was assessed using an Agilent High Sensitivity D1K ScreenTape System. The average sizes of the enriched cDNA fragments were between 272 and 300 bp. The three biological replicates for each condition (negative control, ABA 1 h, ABA 3 h, dehydration 1 h and dehydration 3 h) were pooled into one well, and sequenced on an Illumina HiSeq 2500 with 100-cycle single reads per multiplexed sample.
attributes.tag: GEO Accession
attributes.value: GSM1936765