title: RNA-seq Abrothrix olivacea: adult kidney
design: RNeasy mini kit (Qiagen) was employed for RNA extraction following recommendations of the manufacturer. RNA quantity and purity were assessed with NanoDrop 1000 Technologies spectrophotometer. RNA integrity was checked through electrophoresis in Formaldehyde-agarose 1,2% denaturing gels. Libraries were constructed and sequenced commercially by Macrogen (Korea). Poly-A-based mRNA enrichment and paired-end library preparation were done following the Illumina TruSeqTM RNA sample preparation kit, according to the instructions of the manufacturer. Library sequencing was performed on an Illumina HiSeq 2000 platform (2x100bp paired-end mode)