title: GSM4490921: Sso-RNAseq rep1; Saccharolobus solfataricus; RNA-Seq
library_construction_protocol: The total RNA samples were first fragmented using ultrasound (4 pulses of 30 sec at 4°C). Each  sample was used for three independent cDNA synthesis. First, an oligonucleotide adapter was ligated  to the 3' end of the RNA molecules. First-strand cDNA synthesis was performed using M-MLV reverse  transcriptase and the 3' adapter as primer. The first-strand cDNA was purified and the 5' Illumina  TruSeq sequencing adapter was ligated to the 3' end of the antisense cDNA. The resulting cDNA was  PCR-amplified to about 10-20 ng/μl using a high fidelity DNA polymerase. The cDNA was purified using the Agencourt AMPure XP kit  (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis. The total RNA samples were first fragmented using ultrasound (4 pulses of 30 sec at 4°C). Each  sample was used for three independent cDNA synthesis. First, an oligonucleotide adapter was ligated  to the 3' end of the RNA molecules. First-strand cDNA synthesis was performed using M-MLV reverse  transcriptase and the 3' adapter as primer. The first-strand cDNA was purified and the 5' Illumina  TruSeq sequencing adapter was ligated to the 3' end of the antisense cDNA. The resulting cDNA was  PCR-amplified to about 10-20 ng/μl using a high fidelity DNA polymerase. The cDNA was purified using the Agencourt AMPure XP kit  (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis.
attributes.tag: GEO Accession
attributes.value: GSM4490921