title: RNA-seq of ovis aries: adult tail fat
design: The total RNA from 3 Altay sheep were mixed as one sample to conduct cDNA library. Then the total RNA was treated by DNase , and poly(A) mRNA was isolated using oligo (dT) magnetic beads (Invitrogen, California, USA). The isolated mRNA was cut into short fragments in fragmentation buffer and synthesized the first-strand cDNA using random hexamer primers and reverse transcriptase. Subsequently, the second-strand cDNA were synthesized, purified using the QiaQuick PCR extraction Kit (Qiagen, Germany), and added a ploy(A) fragment to both ends. Then these short fragments were connected with sequencing adapters and separated in gels by electrophoresis. The suitable fragments were selected as templates for PCR amplification to constructed cDNA libraries.