title: Bacterial r16S V4 amplicon from feces of male C57BL/6 mice treated for 4 weeks with oral Vancomycin
design: Duplicate PCR reactions were performed with primers designed to amplify the V4 universal region: 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3). The target fragments were recovered using the AxyPrep PCR Cleanup Kit (Thermo Fisher Scientific). The PCR product was purified using the Quant-iT PicoGreen dsDNA Assay Kit and quantified (Thermo Fisher Scientific). Libraries with the concentration above 2 nmol/L were pooled, loaded on Illumina MiSeq and the MiSeq Reagent Kit v2 (500 cycles) was used for paired-end sequencing (2 x 250 bp).