title: TargetSeq exome of human whole blood DNA
design: Exome library was prepared with Ion Xpress  Plus Fragment Library Kit and Ion TargetSeq  Exome Kit according to the manufacturer's instructions. Genomic DNA fragmentation  (input 1 mkg) was carried out enzymatically with reagents supplied in Ion Xpress  Plus Fragment Library Kit. Library barcoding was conducted with Ion Xpress  Barcode Adapters 1-16 Kit. Exome libraries concentration was quantified using Ion Library Quantitation Kit. Templating and enrichment were carried out on OneTouch  2 System using Ion PI  Template OT2 200 Kit v2 according to user manual. Equimolar pool of two barcoded libraries was prepared prior to templating as two exome libraries were sequenced in one run. Sequencing was conducted on Ion Proton instrument using Ion PI  Sequencing 200 Kit v2 and Ion PI   Chip Kit v2. All reagent listed are from ThermoFisher Scientific.