title: GSM3146196: Naive P14 cells_Spleen_Donor3; Mus musculus; RNA-Seq
library_construction_protocol: Flow sorted cells were lysed and stored in Trizol before extraction of total cellular RNA using the Direct-zol™ RNA MiniPrep kit (Zymo Research). RNA-seq libraries were prepared using SMART-Seq v4 Ultra Low Input RNA reagents (# 634888, Clontech) according to the protocol provided with the reagents beginning with 5ng of total RNA and using 9 cycles of PCR.  150 pg of the resulting cDNA were used for library preparation with the Illumina Nextera XT DNA Library reagents (# 15032354, Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3146196