title: Illumina HiSeq 2500 sequencing; Tissue-specific DNA methylation of the Chicken and Pig
design: Reduced Representation Bisulfite Sequencing with double-enzyme digestion (MspI and TaqaI) and increase size-selected fragments of 40 - 350bp single-end reads of 114bp on a Hiseq2500
library_construction_protocol: https://www.ebi.ac.uk/seqdb/confluence/download/attachments/37489864/RRBS_library_prep_protocol.pdf?api=v2
attributes.tag: assay type
attributes.value: methylation profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 77
attributes.tag: experimental protocol
attributes.value: https://www.ebi.ac.uk/seqdb/confluence/download/attachments/37489864/RRBS_library_prep_protocol.pdf?api=v2
attributes.tag: extraction protocol
attributes.value: https://www.qiagen.com/de/resources/resourcedetail?id=6b09dfb8-6319-464d-996c-79e8c7045a50
attributes.tag: library preparation location
attributes.value: Leibniz-Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, D-18196, Dummerstorf, GERMANY
attributes.tag: library preparation location longitude
attributes.value: 12.227182
attributes.tag: library preparation location latitude
attributes.value: 54.012947
attributes.tag: library preparation date
attributes.value: 2016-07-16
attributes.tag: sequencing location
attributes.value: Leibniz-Institute for Farm Animal Biology (FBN), Institute for Genome Biology, Wilhelm-Stahl-Allee 2, D-18196, Dummerstorf, GERMANY
attributes.tag: sequencing location longitude
attributes.value: 12.227182
attributes.tag: sequencing location latitude
attributes.value: 54.012947
attributes.tag: sequencing date
attributes.value: 2016-09-13
attributes.tag: experiment target
attributes.value: DNA methylation
attributes.tag: library selection
attributes.value: RRBS
attributes.tag: bisulfite conversion protocol
attributes.value: http://www.zymoresearch.de/downloads/dl/file/id/56/d5001i.pdf
attributes.tag: pcr product isolation protocol
attributes.value: https://www.qiagen.com/de/resources/resourcedetail?id=fa2ed17d-a5e8-4843-80c1-3d0ea6c2287d
attributes.tag: bisulfite conversion percent
attributes.value: 99
attributes.tag: restriction enzyme
attributes.value: MspI and TaqaI
attributes.tag: min fragment size selection range
attributes.value: 40
attributes.tag: max fragment size selection range
attributes.value: 350