title: T72_2
design: RNA  libraries for sequencing were prepared using TruSeq Stranded mRNA Sample prep  kit with 96 dual indexes (Illumina, CA, USA) according to the manufacturers  instructions with the following changes. The protocols were automated using  an Agilent NGS workstation (Agilent, CA, USA) using purification steps as  previously described (Borgstrm et al., 2011; Lundin et al., 2010). Libraries  were clustered using cBot and sequenced on HiSeq2500 (HiSeq Control Software  2.2.38/RTA 1.18.61) in HSHOv4 SR50 mode.