title: GSM3083036: WT 1 total; Mus musculus; RNA-Seq
library_construction_protocol: rRNA was depleted from Total RNA using the Ribo-Zero Gold kit (Illumina). 80 ug of RNA was biotinylated according to the following protocol Radle et al. (Rädle et al. 2013). Biotinylated 4sU RNA was isolated and washed using M-270 Streptavidin Dynabeads (Invitrogen), eluted with 100mM DTT, and cleaned up with RNeasy minelute columns (Qiagen). Libraries were generated with the KAPA Stranded RNA-Seq or Stranded HyperPrep library prep kit (Kapa) and sequenced with single-end 50bp reads.
attributes.tag: GEO Accession
attributes.value: GSM3083036