title: GSM2475102: MNase-seq_invitro_nomethylDNA_OxyOctamers_replB; Oxytricha trifallax; MNase-Seq
library_construction_protocol: Oxytricha in vitro MNase-seq protocol: Chromatin was reconstituted in vitro through salt gradient dialysis. Assembled chromatin was adjusted to 50 mM Tris pH 7.9, 5 mM CaCl2 and digested with MNase (New England BioLabs) to mainly mononucleosomal DNA. Mononucleosome-sized DNA was gel-purified and used for paired-end sequencing on an Illumina HiSeq 2500, according to manufacturer’s instructions. MNase-seq (Paired-end sequencing); standard TruSeq Illumina library construction protocol
attributes.tag: GEO Accession
attributes.value: GSM2475102