title: 454 amplicon pyrosequencing of arbuscular mycorrhiza fungi from grassland soil
design: Sampling took place within the vegetation periode of 2011 in a low managed grassland plot in Southern Germany. The sampling scheme is displayed in Suppl. Figure 1 of Regan et al., 2014, SBB. The upper 10 cm of soil were taken, sieved and stored at -20 C. 300 mg of each soil sample was extracted using FastDNA SPIN Kit for Soil (MP Biomedicals, Solon, OH, USA). A semi-nested PCR-approach was used to amplify the small subunit of the fungal 18S rDNA using GLOMERWT0/GLOMER1536 (1. PCR) and NS31/AM1a and AM1b (2. PCR).