title: GSM3984341: Cellular RNA biological replicate_2; Homo sapiens; RNA-Seq
library_construction_protocol: Culture supernatant of iPSC-derived motor neurons was applied to exoRNeasy spin column and RNA was extracted (QIAGEN). Cellular mRNAs were extracted by miRNeasy which uses same spincolumn with exoRNeasy for RNA isolation (QIAGEN) Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.