title: RNA-seq of Arabidopsis Thaiana
design: 5g of total RNA was taken for sequencing. cDNA library was prepared by standard methods of polyA selection followed by random hexamer priming, first strand, and second strand cDNA synthesis. Next sequencing libraries were prepared using standard Illumina adaptors.  15 cycles of PCR was performed to amplify the libraries followed by purification, size selection, quantification on real-time PCR and normalization for whole transcriptome sequencing on Illumina HiSeq 2000.