title: GSM1611686: LT-HSC KO replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: 10,000 purified long-term HSCs and100,000 purified MPPs from WT and Tet1 KO mice were used for RNA isolation. RNA was extracted using RNeasy kit (Qiagen) and PolyA selection using oligo-dT beads (Life Technologies) was performed according to the manufacturer’s instructions. RNA libraries were then sequenced on the Illumina HiSeq 2000 using 50bp paired-end reads. Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM1611686