title: GSM4421205: Col-0_rep 1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: For each replicate sample, approximately 10 plants (whole rosette, excluding roots) per genotype were pooled and immediately frozen in liquid nitrogen and stored at -80 ºC until processing. Wild-type Col-0 and mutant plants were grown in the same tray to minimize tray-to-1tray variation. Three independent samples (biological replicates) were sequenced per genotype. Total RNA was isolated using a Macherey-Nagel NucleoSpin RNA plant kit with Macherey-Nagel on-column DNase treatment to remove genomic DNA. RNA integrity was assessed with a 2100 Bioanalyzer (Agilent Bioanalyzer) and all samples had an integrity score of at least 6.5. Barcoded sequencing libraries were prepared using the BGI transcriptome kit. Normalized libraries were sequenced on the BGI DNBseq platform.