title: GSM4059294: cKO MED1 rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using miRNeasy kit (Qiagen) from purified splenic B cells stimulated in vitro 4 days in RMPI 1640 medium (Lonza) supplemented with 10% fetal calf serum (Lonza) and with 1µg/mL LPS (invivogen) and IL4 (20ng/ml, peprotech), 1 µg of total RNA were depleted from ribosomal RNA, ligated, reverse transcribed and amplified (14 cycles) with the reagents from the truseq stranded total RNA kit (illumina). Libraries were quantified with the Bioanalyzer DNA 1000 Kit (Agilent). 4nM of libraries were then diluted and denatured according to the Illumina recommendations.  Paired-end 150-bp reads were sequenced on an Illumina Nextseq 500 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM4059294