title: RNA-Seq of 24h without inoculation PAV
design: Total RNA were extracted from the seeds using an RNeasy Plant Mini Kit (QIAGEN) according to the manufacturer’s protocol. Messenger RNAs  from the total RNA were isolated using Oligo (dT) and were randomly cleaved into short fragments. Then the first strand cDNAs were synthesized with random hexamer primers and followed by second strand cDNAs synthesis. The cDNAs were purified and ligated to adaptors for paired-end sequencing. Three independent biological replicates for each sample were analyized. The quality and quantity of the libraries were detected using an Agilent 2100 Bioanalyzer and ABI real time RT-PCR. The qualified cDNA libraries were carried out by an Illumina HiSeq 2500 platform with PE125.