title: GSM4421202: WT_SC_biological_rep_1; Mus musculus; RNA-Seq
library_construction_protocol: Mouse hindlimb satellite cells were isolated by Fluorescence Activated Cell Sorting (FACS) by selecting for ITGA7+, Hoechst+, Lin- (CD45-, CD11b-, CD31-, SCA1-) cells. 3000 satellite cells per mouse were immediately lysed by sorting directly into reaction buffer (SMART-Seq V4 Ultra Low Input kit, Takara Bio). RNA from lysed FACS-sorted satellite cells was converted into cDNA libraries using SMART-Seq V4 Ultra Low Input kit (Takara Bio). Illumina sequencing adapters were then added using the Nextera XT DNA library preparation kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4421202