title: GSM4490728: OXA Replicate 2 - RNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: Mouse ears were minced and incubated in digestion media A (5 ml of RPMI 1640 supplemented with 10% FBS, 100uL of a 12.5 mg/ml stock of Liberase TL (Sigma 5401020001), and 50uL 0.1mg/ml of DNAse I (Sigma 10104159001)) for IMQ/IMQ-C samples or digestion buffer B (RPMI 1640 supplemented with 10% FBS, collagenase XI (2 mg/mL; Sigma c9407), and DNAse I (0.1 mg/mL; Sigma 10104159001) for OXA/OXA-C samples at 37 °C for 90 minutes with 200–250 rpm agitation. Cells were dispersed using a tissue dissociator (GentleMACS; Miltenyi Biotec – running program C) for 44s. Single cell suspensions were passed through 40-100 μm filters and washed with  FACS buffer (PBS with 2% FBS). per 10x Single Cell 3' Reagent Kit v2  and v3 manufacturer's protocol ( v2 for sample IMQ-C and IMQ, v3 for sample OXA-C and OXA) and TotalSeq-A Antibodies Protocol with 10x Single Cell 3' Reagent Kit v2 (Biolegend) for OXA-C and OXA CITE-seq libraries
attributes.tag: GEO Accession
attributes.value: GSM4490728