title: GSM4491316: Mut_G1_MNase-seq; Saccharomyces cerevisiae; MNase-Seq
library_construction_protocol: Mononucleosomes were prepared as previously described (Belsky et al, 2015) with the following modifications. Spheroplasting of yeast cells was performed with zymolase at 25ºC for 35 minutes. Conditions for MNase digestion was first optimized with appropriate amount of the enzyme (NEB). Different dilutions of 15 units/μl MNase (NEB) were prepared to determine the best digestion conditions: 1.5-ml tubes contained 0 μl, 1 μl, 2 μl (1:4 dilution in water), 1 μl (1:4 dilution in water), 2 μl (1:16 dilution in water), and 1 μl (1:16 dilution in water) of MNase, to which 400 μl of the resuspended cells was added. Sequencing libraries for ChIP-seq, BrdU-IP-seq and MNase-seq were prepared using Kapa Hyper Prep Kit according to manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM4491316