title: Mreg_UKR cells isolated from donor G
design: All cells were harvested from a separately designated culture vessel (bag or flask) directly in RNAprotect Cell reagent (Qiagen). Isolated cells were allocated 5x106 cells per tube and frozen at -20C. Aliquots of CD14+ monocytes were resuspended in RNAprotect Cell reagent immediately after LP14 purification and frozen at -20C. For prolonged storage cells were transferred to -80C freezer. Two aliquots of frozen cells (5x106 cells each) were sent off to GATC Biotech where RNA purification and next generation sequencing (NGS) were completed. Sequencing was performed on Illumina HiSeq 4000 with 2x150bp paired-end approach. For each sample minimum of 30M reads were delivered.