title: GSM3983238: N2-4-IAA; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Adult worms were collected into 400 µl of M9 salt buffer in RNase-free tubes. After the worms settled to the bottom, the majority of the M9 was removed. 1 ml of Trizol and 100 µl of 0.5 mm of glass beads were added to each tube and immediately frozen at -80 C. Samples were thawed in ice and bead-beaten with a FastPrep-24 5G instrument  with the following settings: speed 4 m/s, 3 cycles, and run time of 20 seconds. After the beads settled at the bottom of the tube, the solution was transferred to a new RNase-free tube. 200 µl of chloroform was added and tubes were vigorously mixed by hand for 15 sec. Samples were then centrifuged at 12000 g for 15 minutes at 4 C and the resulting clear upper-phase was transferred to a new tube, where 500 µl of isopropanol and 1 µl of glycogen were added prior to storing the sample overnight at -20 C. Next, samples were spun at 12000 g for 10 minutes at 4 C, the supernatant was discarded and the pellet washed with 1 ml of fresh 75% ethanol. After 5 minutes centrifugation at 7500 g and 4 C, ethanol was discarded, pellet briefly air-dried and resuspended with 15 µl of RNase-free water.
attributes.tag: GEO Accession
attributes.value: GSM3983238