title: Temperature stress response in skin tissue (heat stress 48hr)
design: Three groups of 3 specimens of N. coriiceps each were sacrificed at 0hr, 24hr, 48hr, and at 24hr of recovery from stresses, and then we dissected each tissues in N. coriiceps. Before dissection, blood sample were collected. Dissected tissues were crashed and immersed in RNAlater at -70C for the next experiments.  For RNA-Seq experiments, we prepared mRNA from blood sample, skin and brain tissue in 3 specimens. Sequencing was performed with Illumina Hiseq 2000.