title: GSM4491318: Mut_G2M_ATAC-seq; Saccharomyces cerevisiae; OTHER
library_construction_protocol: WT and mutant yeast cell pellets were incubated on-ice for 3 min with 50 ul cold ATAC-RSB [10mM Tris HCl (pH7.4), 10mM NaCl, 3mM MgCl2, 1X ROCHE Complete Protease Inhibitor Cocktail, 0.1% IGEPAL CA-630, 0.1% Tween-20, 0.01% Digitonin]. Subsequently, 1mL of cold ATAC-RSB with 0.1% Tween-20 [10mM Tris HCl (pH7.4), 10mM NaCl, 3mM MgCl2, 1X ROCHE Complete Protease Inhibitor Cocktail, 0.1% Tween-20] was added to the mixture. Nuclei were recovered by centrifugation and resuspended in transposase mix for tagementation [1x TTBL and 2.5uL TTE Mix V50 (Vazyme TD501 TruePrep DNA Library Prep Kit V2 for Illumina®), 0.01% digitonin, 0.1% Tween-20, PBS]. The mixture was incubated at 370C for 15 minutes in a Thermomixer(1000rpm). DNA was purified using the Qiagen MinElute PCR Purification Kit. ATAC-seq libraries were amplified with the TruePrep Index Kit V2 for Illumina (Vazyme ) and KAPA HiFi HotStart ReadyMix (Roche). Purified libraries were size selected with AMPure XP beads for 150bp to 800bp fragments, according to manufacturer's protocol. Amplified libraries were analyzed on fragment analyzer and subsequently quantified and pooled for sequencing. 
attributes.tag: GEO Accession
attributes.value: GSM4491318