title: GSM3146646: ML_3; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: genomic DNA extraction using DNeasy Plant kit (Qiagen, Germany). Genomic DNA from three individual plants for both WT and msh1 memory line were used for subsequent Bisulfite methylome sequencing experiment. Genomic DNA Sonicated to 100-300 bp fragments and purified with MiniElute PCR Purification Kit (Qiagen, Germany), incubated at 20 ℃ after add End Repair Mix. Then purified and added a single 'A' nucleotide to the 3' ends of the blunt fragments, purified again and add Methylated Adapter to the 5' and 3' ends of each fragment. Then fragments with 300bp-400bp size-range were purified with QIAquick Gel Extraction kit (Qiagen, Germany) and subjected to bisulfite treatment by using Methylation-Gold kit (ZYMO). Followed with PCR and gel purification (350bp-400bp range fragments were selected). Qualified libraries sequenced pair end on the HiSeq X-ten system.
attributes.tag: GEO Accession
attributes.value: GSM3146646