title: GSM3983830: RBM8A-RIPSEQ-Rep3; Danio rerio; RIP-Seq
library_construction_protocol: RIP-Seq samples were extracted from the IP eluate by Phenol-Chloroform-Isoamyl alcohol extraction. RNA-seq samples were obatined by harvesting whole embryos in TRIZOL. Libraries were prepared using a custom protocol described in detail in Gangras et al., Methods Mol Biol. 2018;1680:1-28. Briefly, a DNA adapter ligated to RNA fragments was used to obtain a reverse transcription (RT) product, which was circularized and used for PCR amplification with primers for Illumina HiSeq sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3983830