title: GSM4491315: Mut_BrdU-IP-seq; Saccharomyces cerevisiae; OTHER
library_construction_protocol: For BrdU incorporation, WT and mutant (orc4-IHΔ) yeast strains bearing BrdU-Inc cassette was generated as previously described (Viggiani et al, 2006). Early S phase arrested cells with BrdU genomic DNA labelling, BrdU-labelled genomic DNA extraction, fragmentation, and immunoprecipitation were performed as described. Validation of BrdU-IP was carried out by qPCR targeting known origins using WT DNA. Sequencing libraries for ChIP-seq, BrdU-IP-seq and MNase-seq were prepared using Kapa Hyper Prep Kit according to manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM4491315