title: Small miRNAs of Arapaima gigas: alevin skin
design: The construction of small RNA libraries were performed in three main steps: : (1) binding of the 5’ and 3’ adaptors; (2) amplification of the construction, and (3) selection of fragments of approximately 20-30 nts in 10% (m/v) polyacrilamide slab gel. The small RNA library was then sequenced by illumine Hiseq 2000.