title: Illumina HiSeq 2500 paired end sequencing; Duroc Pig Macrophages (+/-) LPS
design: RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500
library_construction_protocol: TruSeq Stranded Total RNA Sample Preparation Guide Revision E
attributes.tag: assay type
attributes.value: transcription profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: TRIzol, frozen
attributes.tag: sample storage processing
attributes.value: cryopreservation, other
attributes.tag: sampling to preparation interval
attributes.value: 2
attributes.tag: experimental protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: extraction protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNase_Treatment_20161116.pdf
attributes.tag: library preparation location
attributes.value: Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
attributes.tag: library preparation location longitude
attributes.value: -0.173114
attributes.tag: library preparation location latitude
attributes.value: 58.923953
attributes.tag: library preparation date
attributes.value: 2016-07
attributes.tag: sequencing location
attributes.value: Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
attributes.tag: sequencing location longitude
attributes.value: -3.173114
attributes.tag: sequencing location latitude
attributes.value: 55.923953
attributes.tag: sequencing date
attributes.value: not applicable
attributes.tag: experiment target
attributes.value: total RNA
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: library generation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: read strand
attributes.value: mate 2 sense
attributes.tag: rna purity - 260:280 ratio
attributes.value: 2.04
attributes.tag: rna purity - 260:230 ratio
attributes.value: 2.44
attributes.tag: rna integrity number
attributes.value: 10