title: Bacterial community associated to Monochamus alternatus trachea
design: The aim of this study was to characterize and compare the bacterial community associated to PWD vectors in Europe and East Asia using a culture-independent approach. Twenty-three Monochamus galloprovincialis were collected in Portugal (in two different locations); twelve Monochamus alternatus were collected in Japan. DNA was extracted from the insect’s tracheal system for 16S rRNA gene sequencing through 454 barcoded pyrosequencing.