title: GSM3683660: dWAT-2; Mus musculus; RNA-Seq
library_construction_protocol: Mature dermal adipocytes and inguinal adipocytes were collected from P30 mice using Collagenase IV and DispaseII disgestion combined with the floating centrifuge method.  RNA was harvested using Trizol reagent, and purified with the Qiagen RNA purification kit. 1 µg of total DNAse treated RNA is then prepared for the library construction. RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq Stranded Total RNA Sample Preparation Kit) RNA-Seq-Whole transcriptome
attributes.tag: GEO Accession
attributes.value: GSM3683660