title: GSM2476335: EAC H3K27me3 ChIP-Seq #2; Homo sapiens; ChIP-Seq
library_construction_protocol: Esophageal adenocarcinoma specimens from two patients (both poorly differentiated) and normal human esophageal tissues were crosslinked in 1.1% formaldehyde before chromatin shearing using the Diagenode Bioruptor. The resulting sheared 200-500 bp chromatin fragments were incubated with H3K9me3 (Millipore #07-473) or H3K27me3 (Millipore #07-449) antibody-conjugated Protein A Dynabeads (Life Technology #10002D) overnight. For normalization, an aliquot of sheared chromatin fragments were incubated with antibody against unmodified H3 antibody-conjugated Protein A Dynabeads. Libraries were prepared according to instructions for Illumina Library Kit, Illumina TruSeq v2 (Cat#15026486)
attributes.tag: GEO Accession
attributes.value: GSM2476335