title: Rna seq of K. pneumoniae: grown in normal nutrition
design: The reference genome of Klebsiella pneumoniae 342 was downloaded from NCBI database. (http://www.ncbi.nlm.nih.gov/nuccore/206575712?report=fasta). RNA-Seq is a gene expression profiling technology for quantifying the abundance of previously annotated genes. The RNA transcripts are sequenced and mapped to the reference genome to obtain digital gene expression. There are 5,425 protein coding genes reported in NCBI for Klebsiella pneumoniae 342 strain. The study was carried out by using whole transcriptome differential gene expression pipeline. After the quality filtration process of SOLiD csfasta raw reads, a total of 72,266,571 high quality reads for kp_8hrs sample of Klebsiella pneumonia were used for transcriptome data analysis. The high quality reads of kp_8hrs  sample are present in kp_8hrs.csfasta and its corresponding quality scores file i.e. kp_8hrs_QV.qual . After the quality filtration process of SOLiD csfasta raw reads, a total of 72,266,571 high quality reads for kp_8hrs of Klebsiella pneumonia were used for transcriptome data analysis.  There are 5,425 protein coding genes present in the Klebsiella penumoniae 342 GTF file. These genes were used to study the differential gene expression in both kp_8hrs and kp_24hrs samples respectively by using the whole transcriptome analysis pipeline. The total number of genes expressed after mapping of the reads on the reference genome Klebsiella pneumoniae 342 in kp_8hrs was found to be 4,281 respectively.