title: GSM2059565: TY2205 Young Adult Rep2 RNAseq; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted by freeze cracking embryos by freezein in liquid nitrogen and thawing at 37C water bath five times. RNA was isolated according to the manufacturer's (Invitrogen) Trizol extraction protocol. Purified total RNA RNA was cleaned up through Qiagen RNeasy column. mRNA was purified from .5-10 ug of total RNA using Sera-Mag Oligo(dT) beads (ThermoScientific). cDNA preparation was done in the presence of dUTP to prepare stranded RNA-seq libraries as in PMID:19620258
attributes.tag: GEO Accession
attributes.value: GSM2059565