title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of multiple tissue types and developmental stages of Salvia hispanica (chia)
design: RNA-seq of multiple tissue types and developmental stages of Salvia hispanica (chia)
library_construction_protocol: Tissues were collected from plant in greenhouse and immediately frozen in liquid nitrogen until nucleic acid extraction Total RNA was extracted from frozen plant tissue. Invitrogen Plant RNA Reagent, chloroform, isopropanol, and 8M Lithium Chloride, and DNAse  were used to obtain RNA. Qiagen RNA cleanup kit was used to remove any enzymes used in extraction.  Paired-end libraries were quantified by qPCR and prepared using an Illumina TruSeq Stranded RNA LT kit
attributes.tag: Experimental Factor: seed
attributes.value: developmental stage
attributes.tag: Experimental Factor: seed
attributes.value: organism part