title: Transcriptome Analysis of Rotaria tardigrada
design: 140 Rotaria tardigrada individuals pooled for RNA extraction. Oligo(dT)-primed cDNA was prepared using Clontech/Takara SMART PCR cDNA Synthesis Kit and an Advantage 2 PCR Enzyme System using Invitrogen SuperScript II Reverse Transcriptase. Illumina sequencing was performed in a single HiSeq lane.