title: RNA-seq of Agrobacterium tumefaciens S33: nicotine medium
design: The RNA was extracted with TRIzol Reagent (Invitrogen). The rRNA in the sample was removed by the Ribo-Zero Magnetic kit (G+/G? Bacteria) (EpiCentre). Then the purified mRNA was used for library construction with Illumina Truseq RNA sample prep Kit according to the manufactureríťs protocol.