title: MiSeq of coastal seawater metagenome: LMPB
design: Seawater samples were collected in two coastal ocean observatories (Equatorial Atlantic Microbial Observatory and Blanes Bay Microbial Observatory) during the years 2013 and 2014. DNA extraction was carried out with phenol-chloroform protocol and subsequent purified using Amicon column. PCR amplification was performed using primers for the 16S rRNA gene V3-V4 region and for 18S rRNA gene V4 region. Samples were sequenced in an Illumina MiSeq platform.