title: L.retroversa_2015_April_Rochester: Sample d7_a2
design: "After 1, 7, or 14 days of laboratory exposure to three levels of CO2 (~400, 800 and 1200 ppm) individuals were preserved in RNAlater. Pooled samples of 5-9 individuals were extracted using the E.Z.N.A. Mollusc RNA Kit (Omega Biotek). Three extractions of RNA from each day and treatment combination were sent to University of Rochester (Rochester, NY) for sequencing and library preparation (resulting in 27 unique samples). The samples were prepped with a TruSeq kit and then sequenced on 3 lanes of an Illumina HiSeq 2500 (9 individuals per lane) as a High Output v4 125 bp PE project. Data came from the sequencing facility post trimming. They had used Trimmomatic (v.0.32) to eliminate adapter sequences (2:30:10) and removed low quality scores using a sliding window (4:20), removing both trailing and leading sequences (13) and leaving only sequences with a minimum length of 25 for downstream use. "