title: GSM3983256: B6TS_4C_Tead4-promoter_Rep2; Mus musculus; OTHER
library_construction_protocol: Cells (3x10^6) were cross-linked with 2% formaldehyde, lysed in the lysis buffer (10mM Tris-HCl pH 8.0, 10mM NaCl, 0.2% NP40), digested with HindIII, treated with Klenow fragment and dNTPs to fill in the HindIII digested overhangs, and ligated under a dilute condition. After reversing crosslinks, DNA was purified as a 3C library. The 3C library was digested wth MspI, ligated,  and amplified by PCR tp generate a 4C library. 4C library was amplified by PCR using  two sets of PCR primers. The fist set of primers, Tead4_4C-Hind3F(+)   "5'-TCTTTCCCTACACGACGCTCTTCCGATCTGCCTTCACTATTCCCCAAAA-3'" and Tead4_4C-Msp1R(-)   "5'-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGAACCACTGCTGGGACCTAA-3'", contain a sequence specific to the mouse Tead4 promoter region at the 3' end and a partial sequence of illumina TruSeq adaptor at the 5' end.    The second primers are   "5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'" (TruSeq Universal Adapter sequence) and  "CAAGCAGAAGACGGCATACGAGAT[6bp_index]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3'" (TruSeq Index Adapter sequence).
attributes.tag: GEO Accession
attributes.value: GSM3983256