title: GSM2478115: Nuclear_RNA_KDM5C_KO_mES_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Cells or nuclei were dissolved in Trizol and homogenized with a qiashredder. Direct Ligation of Adaptors to First Strand cDNA (DLAF,PMID: 25607527) Ribosomal RNA was depleted using Eukaryote Ribominus kit. RNA was reverse-transcribed, treated with RNases, ligated to splinted adaptors, treated with USER and PCR amplified. Libraries were pooled and 280-500 bp fragments were gel purified for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2478115