title: Illumina HiSeq 2500 sequencing; RNA-seq of liver and pyloric caeca in salmon fry before and after initial feeding
design: RNA-seq of liver and pyloric caeca in salmon fry before and after initial feeding
library_construction_protocol: Fish was dissected in petri dish with RNAlater under dissecting microscope. Pyloric caeca and liver was collected and put in 2mL eppendof tube with RNAlater, stored at 4 degree for 24h and then transferred to -80 for long term storage. RNA was extracted by using QIAGENE RNeasy Plus Universal Kits Library was prepared by using illumina TruSeq Stranded mRNA Library Prep Kit
attributes.tag: Experimental Factor: organism part
attributes.value: pyloric caeca
attributes.tag: Experimental Factor: sampling time point
attributes.value: before initial feeding