title: GSM4555154: H3K36me3_mESC; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were grown in 15-cm plates until 70% confluency and crosslinked in 1% formaldehyde in growth medium for 10 minutes at room temperature (RT) in a shaker. To stop the fixation glycine was added to a final concentration of 0.125 M and incubated for 5 minutes at RT. Cells were then washed twice with ice cold PBS and harvested by gently scrapping on ice in PBS plus protease inhibitors. Cells from two 15-cm plates were pooled together and centrifuged at 3,400 x g, 4ºC, 5 minutes. Libraries were prepared according to Illumina instructions.
attributes.tag: GEO Accession
attributes.value: GSM4555154