title: pmoA amplicon sequencing from Italian soil using the mb661 reverse primer
design: pmoA amplicons were generated from total DNA extracted from Italian                 paddy soil. PCR products were sequenced using 454 pyrosequencing and pmoA diversity                 was compared to pmoA diversity obtained from clone libraries
library_construction_protocol: total DNA was extracted from 0.3 g of freeze dried                     soil using the MP Fast DNA Spin Kit for soil, pmoA amplicons were generated                     using the primer pair A189f and mb661 including adaptors and barcodes for 454                     sequencing