title: Archaeal community composition of permaforst thaw ponds in subarctic Quebec: Sample S2BSL_2012_16s
design: RNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen). V6-V8 regions of Archaeal 16S rRNA converted to cDNA were PCR amplified using the dual-indexed forward 969 (5'- TYAATYGGANTCAACRCC-3') and reverse primers 1406 (5'-CRGTGWGTRCAAGGRGCA -3'). The resulting 35 Biosamples were sequenced using illumina MiSeq technology.