title: GSM1267148: EBF null total RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from Ebf1 -/- pre-pro-B cells and Rag2 -/- pro-B cells by TRIzol, then subject to DNase digestion to remove residual DNA and cleaned over a RNeasy (Qiagen 74104) column using the manufacturer's 'cleanup protocol' to remove contaminants and deplete RNAs <200 nt. RNA quality was assessed by RNA 6000 nano total RNA (Agilent 5067-1511) analysis on a 2100 Bioanalyzer (Agilent G2938C); all samples had RIN (RNA integrity number) scores = 9.9. RNA samples were depleted of mature ribosomal RNA (rRNA) transcripts with RiboZERO (Epicentre RZH1064) per the manufacturer's recommendation, using 2 μg of RNA as input. RNA depleted of rRNA was converted to indexed, strand-specific RNA sequencing libraries using the ScriptSeqv2 system (Epicentre SSV21106) following the manufacturer's recommendation, using 50 ng of rRNA-depleted RNA as input.  RNA-seq libraries were sequenced on a HiSeq 2000 (Illumina SY-401-1001) to a read depth of ~90,000,000 single end 97 bp reads per sample. 
attributes.tag: GEO Accession
attributes.value: GSM1267148