title: GSM1936148: SAP2; Sphingomonas wittichii; RNA-Seq
library_construction_protocol: Total RNA was isolated from early-to-mid log phase cultures (CFU of 1.0x109 to 1.5x109 cells/mL) using PureLink Mini Kit (Life Technologies, Carlsbad, CA). To ensure complete removal of DNA, RNA samples underwent a two-step DNase I treatment, first with DNase I (amplification grade, Life Technologies) followed by TURBO DNase I (Life Technologies). RNA concentration was measured using Qubit (Life Technologies) and its quality was validated using BioAnalyzer (Agilent Technologies, Santa Clara, CA). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM1936148