title: Miseq fungal ITS2_1
design: primers at 500 nM (gITS7) and 300 nM (ITS4), cycling conditions : 5 min at 95C, followed by 23-26 cycles of 30 s at 95C, 30 s at 56C, 30 s at 72C and final extension at 72C for 7 min.Samples amplified by triplicate together with negative extraction and PCR controls. Ampliconspurified using the NucleoMag NGS Clean-up and Size.