title: GSM3146081: auxin2days.rep2.E [Repli-Seq CTCF Auxin]; Mus musculus; OTHER
library_construction_protocol: Genome-wide RT profiles were constructed as previously described. Briefly, cells were pulse labeled with BrdU for 2 hours and separated into early and late S-phase fractions by Propidium iodide staining and flow cytometry. BrdU-substituted DNA from 40k early and late S-phase cells was immunoprecipitated by anti-BrdU antibody (Becton Dickinson 347580). Sequencing libraries were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370).
attributes.tag: GEO Accession
attributes.value: GSM3146081