title: GSM4491551: ATAC_WT_repeat1; Mus musculus; ATAC-seq
library_construction_protocol: 50,000 sorted DP thymocytes in FACS Buffer were pelleted by centrifugation at 500 RCF for 5 minutes at 4°C in a pre-cooled fixed-angle centrifuge. Then cells were lysed in lysis buffer (10 mM Tris-HCL, 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40) and nucleuses were enriched. 50 ul transposase mixture (10 ul of 5x TTBL, 5 ul of TTE Mix V50, 35 ul of nuclease-free water) (Cat# TD501-01, Vazyme) was added to nucleuses and the pellet was disrupted by pipetting. Transposition reactions were incubated at 37°C for 30 minutes. Transposed DNA was purified using a VAHTS DNA Clean beads (Cat# N411-01-AA, Vazyme) and purified DNA was eluted in 26 ul nuclease-free water. Transposed fragments were amplified and purified according to the standard protocols of TruePrep DNA Library Prep Kit V2 for Illumina (Cat# TD501-01, Vazyme) . The library quality was assessed on the Agilent Bioanalyzer 2100 system. Libraries were quantified by qPCR prior to sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4491551