title: GSM3082858: t_vs_t0_sog1-1_rep2_20min; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: 10 seedlings were collected and total RNA was isolated using the Quick-RNA MiniPrep kit (Zymo Research, Cat# R1055). 2.0μg of total RNA from each genotype was used to generate mRNA-seq libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) for mRNA purification and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530). Sera-Mag Magnetic SpeedBeads (Thermo scientific, Cat# 65152105050250) were used for all the size selection and clean-up steps. The resulting libraries were equally pooled and sequenced with in SE50 mode using a HiSeq2500 machine.
attributes.tag: GEO Accession
attributes.value: GSM3082858