title: GSM2563471: A10: High Fat-Early Exercise (HF-EEX); Rattus norvegicus; RNA-Seq
library_construction_protocol: The left femur was partially sawn at the junction of (1) the distal and the middle thirds and (2) the middle and proximal thirds.The femur was snapped and the resulting mid-diaphysis was centrifuged (10,000 rpm, 4oC, 30s) to obtain the bone marrow. Total RNA was extracted using TRIzol® and cleaned up using RNeasy minikit. A minimum of 1.5 ug of total RNA was sent to Novogene for RNA-seq After QC, mRNA  is enriched from total RNA by Oligo (dT) beads. The mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR
attributes.tag: GEO Accession
attributes.value: GSM2563471