title: GSM4481177: pEry Hi-C data; Mus musculus; Hi-C
library_construction_protocol: Day 10.5 embryos were placed in DMEM-high glucose, 10% FBS, 2mM L-glutamine, 1X MEM-Eagle non-essential amino acids and 12ug/mL heparin. Embryos were decapitated and circulating peripheral blood was allowed to flow into the droplet of the room temperature media from the severed vitelline and umbilical veins. Media was collected and fixed in 2% paraformaldehyde. Cells were stained for CD71 and Ter119, and Hi-C was performed on double positive cells. Single cell libraries were constructed following established protocol (Nagano et al., 2017) using Illumuna Nextera XT kit.
attributes.tag: GEO Accession
attributes.value: GSM4481177