title: Targeted genome sequencing of drug resistance associated loci in Mycobacterium tuberculosis
design: PCR barcoding amplicon (SQK-LSK108) kit and R9.4 flow cells (FLO-MIN106) were used for library preparation and sequencing, respectively. Barcoding PCR was conducted as specified in the nanopore sequencing protocol. A batch of 12 samples were pooled, end-repaired/dA-tailed using NEBNext Ultra II End Repair/dA-Tailing Module and adapter-ligated using Blunt/TA ligation master mix. The library was loaded to the flow cell and sequenced.