title: GSM4490689: GM12878; Homo sapiens; OTHER
library_construction_protocol: To reverse cross-link, samples were treated with 100 μL 20 mg/mL Proteinase K [Thermo Fisher Scientific cat no. 25530049], 100 μL 10% SDS [Thermo Fisher Scientific cat no. 15553027] and 500 μL 20% v/v Tween-20 [Sigma Aldrich cat no. P9416] in a total volume of 2000 μL with nuclease-free water. Samples were incubated in a thermomixer at 56°C for 18 hours with < 1000 rpm rotation (< 30 sec every 15 min) to prevent condensation inside the lid. In order to purify DNA, the sample was transferred to a 5 mL centrifuge tube. The original tube was rinsed with a further 200 μL of nuclease-free H2O to collect any residual sample, bringing the total sample volume to 2.2 mL. DNA was then purified from the sample using a standard phenol chloroform extraction and ethanol precipitation. Purified DNA was SPRI size selected for fragments >1.5 kb, then prepared for sequencing using Oxford Nanopore Technologies SQK-LSK109, and sequenced on ONT's MinION, GridIONor PromethION platforms.
attributes.tag: GEO Accession
attributes.value: GSM4490689