title: GSM4490907: SK01-A1; Mus musculus; RNA-Seq
library_construction_protocol: 4 technical replicates were used to extract total using RNA RNeasy Plus Mini Kit (Qiagen # 74134) according to the manufacturer's protocol. Total RNA was quality-checked using an Agilent BioAnalyzer 2000 instrument.  RNA with an integrity number of greater than 9.5 was used for subsequent analyses. Total RNA was submitted to GeneWiz for RNA-seq analysis. The standard mRNA library preparation TruSeq RNA Sample Prep Kit v2 (Illumina) was used for library preparation. Library concentrations were quantified by Qubit (Invitrogen) and mixed equally for sequencing. Pair-end 150bp sequencing was performed on Illumina NextSeq 500 instrument. Gene count quantifications were performed with RSEM. Standard bulk RNA-Seq (Pair-end 150bp sequencing)
attributes.tag: GEO Accession
attributes.value: GSM4490907