title: GSM3536157: control-rep2: E13.5_NSPCs_control-2_RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. RNA was harvested using Trizol reagent. NEBNext® Ultra™ RNA library prep kit for Illumina® (NEB) was used with 2 ug of total RNA for the construction of sequencing libraries. ChIP's DNA was subjected to end-repair and then was 3'adenylated. Adaptors were ligated to the ends of these 3'adenylated fragments. Next, polymerase chain reaction amplification was to amplify those fragments with adaptors from previous step. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM3536157