title: The test blood DNA sample enriched by Agilent SureSelect 50M and run on one lane of Illumina HiSeq 2000.
design: Libraries were denatured with sodium hydroxide and loaded onto an Illumina cBot for cluster generation according to the manufacturer's recommended protocols (TruSeq PE Cluster Kit v2, Illumina Cat# PE-401-2001). Lane 5 of each flowcell was reserved for a PhiX control (Illumina Cat# FC-110-3001). The primer-hybridized flowcells were then transferred to HiSeq 2000 sequencers and paired-end sequencing was performed with TruSeq SBS kits (Illumina Cat#FC-401-1001) in a 2 x 101b mode.