title: GSM4420060: undifferentiated 1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol and column-based extraction (Zymogen, Direct-Zol RNA kit) Libraries were genertaed using the illumina TruSeq Staranded Total RNA Preparation Kit with Human/Mouse/Rat Ribosomal RNA depletion as per standard protocol, using 1ug Total RNA input per sample. Libraries were validated using the Labchip GX Touch 24 (Perkin Elmer) and quantified with the Qubit 2.0 (Thermofisher Scientific). Sequencing was performed on a NextSeq 500 using the High Output 150 cycle v2.5 kit.
attributes.tag: GEO Accession
attributes.value: GSM4420060