title: GSM3901309: ATAC-seq_KO_Rep2; Mus musculus; ATAC-seq
library_construction_protocol: RNA from cells or tissues was harvested using Trizol reagent following purinfication by Qiagen Rneasy microKit.  For ChIP-seq, lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ATAC-seq was optimized in liver after several modifications from original Buenstero protocol (Curr Protoc Mol Biol. 2015) Libraries for RNA-Seq were prepared with KAPA Stranded RNA-Seq Kit on RNA isolated from livers of chow diet feeding mice with AAV-GFP or AAV-Cre transduction. ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems). ATAC-Seq libraries were prepared using the Nextera Tn5 Transposase and DNA library preparation kit (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM3901309