title: RNA - Seq of sorghum: stem during sugar accumulation
design: Stem tissues were collected from flag leaf stage to 30 days after flowering from the upper internodes of field-grown sorghum plants Rio, BTx406 and R9188. Per timepoint and sorghum genotype, three biological replicates were harvested consisting of nine plants. Total RNA was extracted using the TRIzol reagent. Libraries were prepared using the Illumina TruSeq RNA kit with Poly-A selection.