title: GSM2825311: 293_MOV10KD_3; Homo sapiens; RNA-Seq
library_construction_protocol: Medium was removed from cells and RNA was extracted using 1 ml TRI-reagent per single well.  RNA was treated with Ambion TURBO DNase (Life Technologies) according to the producers' recommendations. Following DNase treatment the RNA samples were all supplemented with equal amounts of ERCC RNA spike-in mix (Thermo Fisher Scientific) followed by depletion of rRNA by using Ribo-Zero H/R/M Kit (Epicentre) according to the manufacturer's protocol. Strand-specific RNA libraries were prepared in triplicate using KAPA Stranded RNA-Seq Library Preparation Kit for Illumina platforms (KAPA Biosystems) according to the manufacturer's protocol using 8 mins at 95 °C for RNA fragmentation and 700 μM adapters for amplification. We used 7 cycles of amplification for generation of the libraries. Libraries were analysed by capillary electrophoresis using Bioanalyzer 2100 (Agilent). All libraries were ~250 bp in length (after subtracting 120bp adapter sequences).
attributes.tag: GEO Accession
attributes.value: GSM2825311