title: GSM2898140: LAMP-2 KO; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent. Illumina TruSeq Stranded mRNA Sample Prep Kit (Cat# RS-122-2101) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA Libraries were constructed using Illumina TruSEQ mRNA Sample Prep Kit (Cat# RS-122-2101). Total RNA is combined with RNA purficiation beads to bind PolyA RNA to oligodT magnetic beads.  mRNA is eluted and converted to double stranded DNA.  A Tailing, adapter ligation, and PCR amplification using 15 cycles is done to complete the library construction.  Libraries are quantitated via Qubit, analzyed on a Bioanalyzer Tape and diluted to appropriate concentration to run on HiSEQ 4000 High Throughput Flow Cell.
attributes.tag: GEO Accession
attributes.value: GSM2898140