title: RNA-seq of Pangasianodon hypophthalmus: adult spleen
design: Total RNA was extracted from spleen tissue of striped catfish using Trizol reagent (Invitrogen). Then, cDNA library was constructed using TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the manufacturer s procedure. The cDNA library was sequenced using Illumina Hiseq 2500 sequencing platform.