title: RNAseq of C. neoformans pmc1 mutant cultured for 3 h in host mimicking conditions replicate 3
design: ÊCryptococcus neoformans pmc1 mutant cells cultured in DMEM supplemented with 10 % heat-inactivated FBS, 1 mM l-glutamine, 1 mM sodium pyruvate and 1 % nonessential amino acids at 37 ¡C with 5 % CO2 for 3 h. The analysis was conducted with biological and technical triplicates. Yeast cells were washed three times with PBS and lyophilized for subsequent RNA extraction using an RNeasy¨ Mini kit (Qiagen). Poly (A) RNA was purified with a Dynabeads¨ mRNA purification kit (Thermofisher Scientific), and RNA quality was assessed with a Bioanalyzer 2100 system (Agilent Technologies). cDNA libraries