title: GSM4491082: D18-1730058-5-3240A; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed after 48 hours in 500 μL TRIzol and pipetted repeated through a P1000 to sheer DNA. RNA was extracted by adding 180 μL chloroform, vortexing for 15 seconds, and then centrifuging at 12,000 g for 15 minutes at 4°C. The top, clear, RNA-containing phase was then transferred to a new tube, mixed 1:1 with 100% Ethanol and purified using a Direct-zol RNA MicroPrep kit. RNA-seq libraries were prepared from 50 ng of RNA using Kapa mRNA Hyperprep Kits. RNA-Seq analyses were performed by The MIT BioMicro Center. Library sequencing was multiplexed on two lanes of a HiSeq 2000 (Illumina) using paired-end sequencing for 40 bp.
attributes.tag: GEO Accession
attributes.value: GSM4491082