title: Characterization of invasive pneumococcal isolates recovered from individuals in the United States through the Active Bacterial Core surveillance program
design: Shearing protocol - 500 bp protocol using sonication (Covaris) Library preparation kit - Ovation Rapid (Nugen) Index - dedicated read Library QC   KAPA qPCR quantification (KAPA Biosystems) Sequencing instrument - Miseq Sequencing kit - Illumina MiSeq Sequencing kit v2 (500 cycles)