title: Illumina HiSeq 2000 paired end sequencing
design: Illumina fosmid-end sequencing
library_construction_protocol: Genomic DNA cloned in Lucigen pNGS_FOS vector (http://www.lucigen.com/docs/vector/pNGS.html or http://www.snapgene.com/resources/plasmid__files/lucigen_vectors/pNGS_FOS_(linearized)/), digested with CviQI (GTAC cut site) and ligated together bringing fosmid insert ends into proximity. PCR with Illumina primers followed by clean up and adaptor ligation.