title: RNA-Seq of templates and products from T7 RNAP-RNA replication (Experiment/Result: Role of 2-way repeats and 4-way repeats in RNA replication by T7 RNAP)
design: Replicate 1, library_source= in vitro RNA population; For RNA-seq library preparation, there are four main molecular biology steps: 1) 3 ligation of ssDNA adapter to RNA, 2) Reverse transcription (using a primer that is complementary to the ssDNA adapter used in step 1), 3) Circularization of cDNA and 4) PCR. For each sample we sequenced, we used gel electrophoresis to visualize and extract intermediate products during library preparation following each of the steps of 3 ligation, reverse transcription and PCR; fastq data uploaded corresponds to raw data after demultiplexing (adapters not trimmed in uploaded data); further details on library preparation, data processing, size selection of RNAs for sequencing, and adapter sequences including molecular barcode sequences and positions,  can be found in the associated manuscript