title: GSM4555277: DKO PT RB2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated at different stages of tumor development in DKO and TKO mice: fallopian tubes at premalignant stage (Pre), early-stage fallopian tube tumors (ET). Additionally, RNA was isolated from normal fallopian tubes (Ovid, oviducts) of respective control mice for DKO and TKO mice. Total RNA was isolated using TRIzol (Invitrogen) and purified using RNeasy MinElute Cleanup Kit (Qiagen, Germantown, MD, USA), according to manufacturer's instructions. Total RNA (1 -1.2μg) was used for mRNA library preparation. Library preparation was performed using the TruSeq Stranded mRNA HT Library preparation kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4555277