title: GSM4218543: D: mice in Diarrhea models; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA). RNA concentration and total quantity were detected by Invitrogen Qubit 3.0 Spectrophotometer (Thermo Fisher Scientific, USA); RNA purity was checked using the Nanodrop 2000 (Thermo Fisher Scientific, USA); and RNA integrity was checked by Agilent 2100 Bioanalyzer (Agilent Technologies, USA). mRNA was obtained through purifying total RNA, and was broken into fragments of 100–300 bp. Reverse Transcribe was performed to synthetize First Strand cDNA using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific, USA). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Then addition of adenlylate 3' ends, connection sequencing adapters, selection library size, PCR amplification and checking library quality were performed in turn. Sequencing was performed on the Illumina Hiseq 2500 (Illumina, USA) and raw data was obtained. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4218543