title: RNA-Seq of Acinetobacter baumannii
design: A. baumannii was cultured in M9 minimal medium supplemented with pyruvate or IAA. The logarithmic phase bacteria were harvested by and for RNA purification. RNA was isolated from bacteria with an RNeasy Protect Bacteria Mini Kit (QIAGEN) following the manufacturer's instructions. After RNA extraction, rRNA was depleted and the mRNA were used for RNA-Seq following the manufacturer's protocols for Illumina HiSeq 2000 platform (Illumina)