title: GSM3083160: Young Myeloid-biased HSCs #1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the Zymo Direct-Zol RNA MiniPrep Plus (cat# R2070, Irvine, CA) as per the manufacturer's instructions. Total RNA was quantified and 260/280 ratios determined using Nanodrop. RNA sequencing and library preparation was performed by the UCLA Technology Center for Genomics and Bioinformatics using the KAPA Stranded mRNA-seq kit (Roche Sequencing, cat#KK8421, Pleasanton, CA, USA), according to the manufacturer's instructions. The work flow consisted of mRNA enrichment, cDNA generation, end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on the HiSeq3000 System for a paired-ended 150bp run (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3083160