title: GSM3082830: PrimPol rep 1; Drosophila melanogaster; Gallus gallus; OTHER
library_construction_protocol: Upon mixing the DT40 cells with the S2 cells (3.75:1 ratio) , the nuclear fraction was extracted (then treated with rnaseH for the control),  genomic DNA containing containing DNA:RNA hybrid purified and fragmented with restriction enzyme digest. Soluble contaminating RNA was digested with Rnase A treatments and DNA:RNA hybrids immunoprecipitated with S9.6 antibody. Following low, high, LiCl and TE buffer washes of Protein-G captured immunocomplexes, DNA was eluted, phenol:chloroform purified and fragmented to 300 bp average size with Covaris M220 Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) as per provided instruction for low input DNA samples, from approximately 30 ng of immunoprecipateted DNA. Size selection was ommited and total of 7 PCR amplification cycles.
attributes.tag: GEO Accession
attributes.value: GSM3082830