title: GSM4670330: C6-45 Post1; Homo sapiens; RNA-Seq
library_construction_protocol: Cryopreserved PBMCs of the AML patients were quickly thawed and carefully transferred to the 35ml of pre-warmed α-MEM media (22 °C). Cells were pelleted by centrifugation at 300xg for 5 min at 22 °C, and then washed with 30ml of 0.04% BSA in PBS (22 °C). Live cells were collected by sorting with FACS after staining with CD45 and PI. RNA libraries were prepared for sequencing using Single Cell 3' Reagent Kit v3 (10x genomics).
attributes.tag: GEO Accession
attributes.value: GSM4670330