title: GSM4121259: 1_9I: Small RNAs from ovaries of 1.9I transgenic strain; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: smallRNA-seq: Total RNA was isolated by Trizol (Invitrogene) and size-fractioned by PAGE (19-29 nt). smallRNA-seq: Libraries of small RNAs 19-29 nt were constructed as previously described in (Muerdter et al. 2012) GRO-seq: Nuclei were purified from homogenized ovaries, and were used for run-on reacton with Br-UTP in vitro (Rozkov et al. 2013). GRO-seq: De novo synthesized RNA was subjected to library preparation for RNA-seq.
attributes.tag: GEO Accession
attributes.value: GSM4121259