title: amoA amplicon sequencing of Antarctic soil
design: DNA was extracted from 2 g soil from each sample (n=27, 12 of each temperature regime, with three controls) using the MoBio PowerSoil DNA Isolation Kit (MoBio, Carlsbad, CA, USA) as per the manufacturers instructions. DNA was amplified by single-step PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) using the universal bacterial primer set bac-amoA-1F and bac-amoA-2R which target the 332 to 344 and 802 to 822 regions flanking the bacterial ammonia monooxygenase (amoA) gene. Amplicons from each sample were pooled in equal concentrations and purified using Agencourt AMPure Beads (Agencourt Bioscience Corporation, MA, USA). The samples were then submitted for 150 x 2 bp paired-end sequencing on an Illumina MiSeq platform