title: multiplex amplicon sequencing:bacteria, fungi, plants, fish, animals: environmental sample
design: DNA was extracted using a range of commercially available extraction kits from soil, leaf litter, stream water, stream biofilm, stream sediment, kick-net samples. Environmental DNA extracts were PCR amplified using five pairs of primers, targeting 16S rRNA for bacteria, ITS2 for plants, ITS2 for fungi, 12S rRNA for fish and COI for animals. Amplicons were pooled before sequencing using 2 x 300 bp chemistry