title: GSM2475111: Oxy_polyA_RNAseq_replA; Oxytricha trifallax; RNA-Seq
library_construction_protocol: Oxytricha polyA+ RNAseq protocol: Vegetative Oxytricha cells were lysed in TRIzol reagent (Thermo Fisher Scientific) for total RNA isolation according to manufacturer’s instructions. Poly(A)+ RNA was then purified using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). The Oxytricha poly(A)+ RNA was prepared for RNA-seq using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Illumina). polyA+ RNAseq (Single-read sequencing); ScriptSeq v2 RNA-Seq library preparation protocol
attributes.tag: GEO Accession
attributes.value: GSM2475111