title: ITS amplicon: Bulk soil
design: Potting mixture samples were collected from biochar amended and non-amended treatments. DNA were extracted and PCR amplification of ITS1 gene fragments was performed using ITS1F and ITS2 primers. PCR amplicon was sequenced using Illumina MiSeq technology.  Barcode and  primer sequences were trimmed, and data was analyzed using qiime pipeline version 1.9.1.