title: Illumina NovaSeq 6000 sequencing; Single nucleus RNA sequencing in mouse brown adipose tissue at room temperature, cold condition and thermoneutrality
design: Single nucleus RNA sequencing in mouse brown adipose tissue at room temperature, cold condition and thermoneutrality
library_construction_protocol: The RT mice were housed in 23 C; the CE mice were housed in 8 C for 4 days before sacrifice; the TN mice were housed in 30 C for 11 weeks before sacrifice. iBAT of Adipo-NucRed transgenic mice are harvested. We used the RFP and DAPI to collect double positive nuclei. DAPI was stained, RFP was tagged with nuclear localization sequence and expressed in cells that have been expressing Adipoq. The transgenic mouse model is: Rosa26-loxp-STOP-loxp-NLStdTomato x Adipoq-Cre We applied a protocol from Habib et al., Nature Methods 14, 955–958(2017) Standard 10x was applied
attributes.tag: Experimental Factor: temperature
attributes.value: 8