title: NextSeq 500 sequencing; microRNA-seq of miRNAs from control and DIS3L knockdown HeLa cells
design: microRNA-seq of miRNAs from control and DIS3L knockdown HeLa cells
library_construction_protocol: HeLa cells were transfected with control or DIS3L targeting siRNAs and harvested in RNA lysis buffer (Quick RNA miniprep kit from Zymo Research) after 72 hours. RNA was extracted according to the manufacturer's protocol (Quick RNA miniprep kit from Zymo Research). 1 microgram of total RNA was used for library preparation using the NextFlex Small RNA-seq kit V3 from Bioo Scientific.
attributes.tag: Experimental Factor: RNA interference
attributes.value: scrambled siRNA