title: Nanopore  genome sequencing of Holothuria scabra:male muscle
design: Total RNA is used to Construction of RNA-seq librariesmRNA was enriched from the total RNA using oligo(dT)magnetic beadsfinallythe average insert size for the final cDNA library was 350 bp (50 bp). At last, we performed the paired-end sequencing on an Illumina Hiseq X-Ten (LC Bio , China) following the vendor's recommended protocol.