title: GSM4420163: WT 3; Mus musculus; RNA-Seq
library_construction_protocol: Dissociated cortical cells were resuspended in PBS, filtered through a 70 micron cell strainer followed by a 40 micron tip strainer, and counted with a Countess instrument (Life Technologies). The cells were further diluted to the final concentration (80,000 cells/mL) with OptiPrep (Sigma) and PBS, and the final concentration of OptiPrep was 15% vol/vol. A total of 2,000 to 5,000 cortical cells were collected per mouse using an InDrop setup. Libraries were barcoded independently for aliquots containing ~1,200 cells, and constructed following the InDrop protocol ​(Klein et al., 2015; Zilionis et al., 2017)​.
attributes.tag: GEO Accession
attributes.value: GSM4420163