title: GSM1694285: Duo_normal diet_1,25_6; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using the TRI-Regent protocol (MRC, Cincinnati, OH). Eight μg of total RNA was DNase1 treated (Invitrogen) then ribosomal RNA was depleted using the RiboMinus Eukaryote Kit (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions Directional RNA-seq libraries were prepared with the Epicentre (Madison, WI) ScriptSeq v2 RNA-seq Library Preparation Kit per manufacturer’s instructions.  Ribosomal RNA depletion (RNA 6000 Nano Kit) and final library quality (High Sensitivity DNA kit) was analyzed with the Agilent Bioanalyzer (Agilent, Santa Clara, CA).
attributes.tag: GEO Accession
attributes.value: GSM1694285