title: GSM4670649: AJ100: Human sigmoid mucosa - Lean patient; Homo sapiens; RNA-Seq
library_construction_protocol: Sigmoid mucosal biopsies were cryopreserved and FACS isoleted, using a BD FACSAria II cell sorter. A QC analysis was completed using a Vi-Cell XR to evaluate quality and compatibility of cells. Reverse transcription was conducted using Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10X Genomics). cDNA was recovered. Pre-amplified cDNA was further subjected to library preparation. Libraries were sequenced on an Illumina Hiseq 4000
attributes.tag: GEO Accession
attributes.value: GSM4670649