title: RNA-Seq of Arabidopsis thaliana: seedlings, 35::DEK3, short days, time - ZT4, 17C
design: Total RNA was isolated from 30 mg of grinded seedlings using the MagMAX-96 Total RNA Isolation kit (Ambion, AM1830), following the manufacturers instructions. RNA quality and integrity was assessed on the Agilent 2200 TapeStation. Library preparation was performed using 1 ug of high-integrity total RNA (RIN>8) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, cat. No. NEB #E7420). Libraries were sequenced in-house on an Illumina NextGen 500 sequencer using paired-end sequencing of 75 bp in length.