title: GSM3983877: SEM_RNA_PROM1_shRNA_KD_replicate_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from 10 million SEM cells using the Directzol RNA miniprep kit (Zymo R2050). Poly A purification was conducted using the NEB Poly(A) mRNA magnetic isolation module as per the manufacturer's protocol. DNA libraries were prepared using the NEB Next Ultra II Directional RNA library preparation kit for Illumina (E7765).