title: GSM2478024: LECS_CM_WM164_NEG_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified using RNeasy Mini kit (Qiagen) and digested with DNase (Qiagen). 1 µg of total RNA samples was used. Average sample RNA Integrity Number was 9.8 when assayed on an Agilent 2100 Bioanalyzer. PolyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq Stranded mRNA Sample Preparation Part # 15031047 Rev. D" kit (this kit incorporates dUTP during 2nd strand cDNA synthesis, which implies that only the cDNA strand generated during 1st strand synthesis is eventually sequenced). Adapter-ligated library was completed by PCR with Illumina PE primers (10 cycles). The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina HiSeq2500 by following manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM2478024