title: 2b-RAD sequencing for Chlamys farreri
design: 2b-RAD sequencing sample of a Chlamys farreri mapping population was consisted of two parents and 96 progenies.Each individual was separately constructed to a library, totally 98 libraries. Each female and male parent library was separately sequenced in one lane on the first run.Each progeny library had its own barcode sequence. Every 24 progenies libraries were mixed equally and sequenced in one lane in another run, thus produced four lanes data of progenies.Barcodes sequences were:    Barcode1  CGATGACA  Barcode2  TACGAGCA  Barcode3  CTACGACA  Barcode4  AGTCAGCA  Barcode5  CATAAGCA  Barcode6  GCATGACA  Barcode7  CAAGCACA  Barcode8  ACAGAGCA  Barcode9  CTCCAGCA  Barcode10  TCACGACA  Barcode11  ATGTCACA  Barcode12  ATCAGGCA  Barcode13  TTAGGCCA  Barcode14  TGACCACA  Barcode15  TAGTCACA  Barcode16  TACGCA  Barcode17  CTACCA  Barcode18  AGCACA  Barcode19  ACAGCA  Barcode20  TTAGCA  Barcode21  TGACCA  Barcode22  GTCACA  Barcode23  AGCA  Barcode24  CA