title: fish pond water
design: The quality of the DNA was analyzed on Agilent 2100 bioanalyzer (Agilent, USA). After quantitation, the DNA were mixed in equimolar ratios based on concentration according to the sequencing requirements. PE libraries were constructed by PCR with TruSeqTM DNA Sample Prep Kit. Illumina MiSeq sequencing were performed at Majorbio Bio-Pharm Technology Co., Ltd., Shanghai, China.