title: SMRT seq of beta lactamase TEM-1: molecular evolution by epPCR, Pacbio Sequel sequencing
design: We mutagenized the pUC19 TEM-1 beta lactamase. The library was subject to consecutive cycles of mutation, selection and amplification through the use of error prone PCR  and growth in selective (Ampicillin) semisolid media. We called the library at the end of each cycle a generation of molecular evolution. In total, 12 generations were made and the first, fifth, and twelfth generation were sequenced.