title: GSM2478040: CA16-0h; Homo sapiens; miRNA-Seq
library_construction_protocol: Samples from the previously mentioned groups were sent to the National Engineering Center for Biochips in Shanghai. miRNA was isolated from each group using a mirVanamiRNA Isolation Kit (Ambion, USA) in accordance with the manufacturer’s guidelines. RNA purification was carried out after RNA isolation using a miRNeasy Mini kit (Qiagen, Germany) following the manufacturer’s instructions. The quality and integrity of the purified RNA were determined by capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies, USA); only samples with an RNA integrity number (RIN) value greater than 7 were retained for miRNA profile analysis. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2478040