title: GSM4120377: ATAC_Mono_LP_Toxo_rep2; Mus musculus; ATAC-seq
library_construction_protocol: 20000 sorted monocytes were washed with cold PBS and resuspended in cold lysis buffer (10 mM Tris·Cl, pH 7.4, 10 mM NaCl,3 mM MgCl2, 0.1% (v/v) Igepal CA-630). Nuclei were subject to transposition reaction in 1x TD Buffer containing 2.5 μl transposase Nextera enzyme and DNA was purified. Purified DNA was amplified by PCR with 10-12 cycles. Amplified libraries were purified and quality controlled.
attributes.tag: GEO Accession
attributes.value: GSM4120377