title: GSM4419347: DASH_100_1000; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; RNA-Seq
library_construction_protocol: Cultures were pelleted by centrifugation and RNA was extracted with Trizol. To remove contaminating genomic DNA, samples were further treated with 0.25 U of DNase I (Fermentas) per 1 µg of RNA for 45 min at 37°C, followed by phenol-chloroform extraction and ethanol precipitation of RNA. Libraries were generated with NEBNext Magnesium RNA Fragmentation Module (# E6150S) in combination with NEB NEBNext Multiplex Small RNA Library Prep Kit for Illumina (#E7560S) or Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (#634411). Where indicated, ribosomal cDNA was depleted by the DASH procedure: Depletion of Abundant Sequences by Hybridization using target specific sgRNAs and Cas9 to remove unwanted high-abundance species in sequencing libraries.
attributes.tag: GEO Accession
attributes.value: GSM4419347