title: GSM3983868: Day4_EtOH.1; Mus musculus; RNA-Seq
library_construction_protocol: At Day 2 and Day 4 post-induction, mESCs were harvested in TRIzol and total RNA purified using the PureLink RNA Mini Kit (Life Technologies) with on-column DNase treatment. Total RNA was quantified on a Nanodrop and RNA quality was assessed on an Agilent 2100 Bioanalyzer using the RNA 6000 Nano kit (Agilent Technologies). Libraries were constructed from 1 mg total RNA using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina). The resulting libraries were assessed by High Sensitivity DNA Assay on a Bioanalyzer and quantitative real-time PCR using the KAPA Library Quantification Kit (Roche).