title: GSM3682618: V_NT_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of hESC-CMs were isolated by illustra™ RNAspin Mini Isolation Kit (GE Healthcare). RNA quality was assessed by measuring the RNA integrity number (RIN) using Bioanalyzer (Agilent Technologies). All 12 samples for library preparation have RIN within 8 to 10. 250ng of total RNA was prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). RNA sequencing was performed on Illumina NextSeq 500 with 2x43 paired_end sequence reads in the depth of 20 millions.
attributes.tag: GEO Accession
attributes.value: GSM3682618