title: GSM4420172: dgul1_rep3; Trichoderma reesei; RNA-Seq
library_construction_protocol: Total RNA was prepared from the mycelia by grinding under liquid nitrogen with a mortar and pestle, followed by extraction using the RNAisoTM reagent (TaKaRa, Japan) according to manufacturer's protocol. First, mRNA molecules were enriched with oligo(dT) beads and disrupted into ~300 bp fragments. Then, cDNA was synthesized using mRNA as a template. The library was prepared for sequencing by PCR and size selection.
attributes.tag: GEO Accession
attributes.value: GSM4420172