title: GSM2563826: BA-2-CD4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted by using Trizol Reagent, and was purified by RNeasy Micro Kit. Library preparation was performed as previously described (Zhang et al., 2014). We used 200 ng RNA per sample as input material for the RNA sample preparations. cDNA libraries were constructed by using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer's instruction, and the products were purified by AMPure XP system and library quality was determined on the Agilent Bioanalyzer 2100 system.
attributes.tag: GEO Accession
attributes.value: GSM2563826