title: GSM4419715: Rep 1 Day 0  [Day0_1]; Bos taurus; RNA-Seq
library_construction_protocol: Cortical strips harvested and frozen at day 0 or after 2 and 4 days of culture were homogenized over liquid nitrogen. Total RNA was extracted using Trizol Plus RNA Purification Kit (Invitrogen, Carlsbad, CA) following manufacturer instructions. Genomic DNA was removed by a complementary on-column procedure (Life Technologies). Concentration of RNA was assessed using the Nanodrop and quality of RNA were assessed with the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) or Caliper LabChip GX Analyzer. Barcoded sequencing libraries were prepared using the QuantSeq FWD kit (Lexogen, Vienna, Austria) for multiplexed sequencing according to the recommendations of the manufacturer using UDI-adapter and UMI Second- Strand Synthesis modules (Lexogen). The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a LabChip GX system (PerkinElmer, Waltham, MA). The libraries were quantified by fluorometry on a Qubit fluorometer (Life Technologies) and pooled in equimolar ratios. Libraries were sequenced in one lane of the HiSeq 4000 sequencer (Illumina, San Diego, CA) with single-end 100 bp reads. 3'-tag RNAseq
attributes.tag: GEO Accession
attributes.value: GSM4419715