title: GSM3145910: MYB83 Induced Root; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: For Drop-seq runs, plants were grown as described above and roots were harvested then protoplast as previously described (Benfey & Scheres, 2000; K. Birnbaum et al., 2003; Macosko et al., 2015). Individual cells were resuspended in Solution A containing: 600 mM Mannitol, 2mM MgCl2*6H20, 0.10% BSA, 2mM CaCl2*2H20, 2mM MES Hydrate, 10mM KCl and pH to 5.5 with 1M Tris.  Each sample of cells was filtered through a 40 μM Cell strainer (BD Falcon) into a 50 mL Falcon tube. Cells were then counted and diluted to 100-180 cells per μl immediately before Drop-seq (Supplemental Table 2). All Drop-seq steps followed the standard protocol outlined by the McCarroll Lab. The droplet diameter ranged from 0.8 - 0.975 nl (Supplemental Table 2) and Drop-seq was run until 1 mL was collected according to standard Drop-seq protocol. cDNA was amplified to 13-17 cycles and double-purified in 6x volume of Agencourt AMPure XP beads. Tagmentation was carried out with 1200pg of DNA. Samples where ran on the Bioanalyzer  (Supplemental Figure 3) DropSeq + Nextera
attributes.tag: GEO Accession
attributes.value: GSM3145910