title: GSM2827137: 1b-14-8; Mus musculus; RNA-Seq
library_construction_protocol: After 16 h, cells were washed with PBS and lysed with Trizol (TRizol reagent, Thermo Fisher, 15596026). For phase separation, chloroform was added. Total RNA in the upper layer was precipitated with isopropanol and washed with 70% ethanol. The sample was DNase (Turbo DNase, Thermo Fisher, 40226) treated, and purified total RNA was phenol/chloroform extracted. Strand specific Illumina TruSeq RNA library preparation kit v2 with polyA enrichment was used to construct libraries from total RNA
attributes.tag: GEO Accession
attributes.value: GSM2827137