title: GSM3001113: Scx siRNA rep3; Equus caballus; RNA-Seq
library_construction_protocol: Thirty minutes after completion of the final strain cycle, cells were collected into guanidine isothiocyanate-phenol solution (TRIzol® Reagent, Invitrogen, Carlsbad, CA) for RNA isolation by column purification according to manufacturer instructions (Direct-zol Microprep, Zymo Research, Irvine, VA). RNA was evaluated both spectrophotometrically for quantity (NanoDrop, Thermo Scientific, Waltham, MA) and by electrophoresis for RNA integrity (Bioanalyzer, Agilent, Santa Clara, CA). 1 ug of total RNA per sample was enriched for polyA RNA (PrepX PolyA mRNA isolation kit, Wafergen, Fremont, CA) and converted to cDNA libraries (PrepX RNA-seq for Illumina Library Kit, Wafergen). Libraries underwent 13 rounds of PCR to generate the final cDNA libraries for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3001113