title: RNAseq of Etheostoma spectabile: eye, gonads, muscle, liver, fins, and brain from adult male and female, and entire fry
design: Equal amounts of RNA were pooled from one whole fry and each of the adults' tissues to obtain a total of 1 g RNA. An RNAseq library was prepared and sequenced at the Biotechnology Center at the University of Illinois. A TruSeq Stranded mRNAseq Sample Prep kit (Illumina) was used to prepare the RNAseq library. Resulting cDNA fragments ranged from 100 bp to 900 bp, with an average of 400 bp. The library was quantitated by qPCR. Sequencing of paired-end 250 bp reads was done on two lanes of an Illumina HiSeq 2500 using a HiSeq Rapid SBS sequencing kit (version 2).