title: NextSeq 500 sequencing; Effects of anti-androgenic compounds on zebrafish insulin mutants
design: Effects of anti-androgenic compounds on zebrafish insulin mutants
library_construction_protocol: insulin mutant embryos were obtained from two different crosses (biological replicates). Embryos were grown at 28 degrees C in egg water.  10 animals per sample were pooled, water was removed and Trizol was added.  Animals were treated with 1% DMSO or Flutamide (10 micromolar) or Cyproterone (10 micromolar) from 84 to 120 hours post fertilization (hpf).  Total RNA was isolated from 120 hpf zebrafish using the RNA Clean & Concentrator kit (Zymo Research) combined with DNase digestion (RNase-free DNase Set, Promega) to avoid contamination by genomic DNA. 3µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following manufacture's low sample protocol (Illumina)
attributes.tag: Experimental Factor: compound
attributes.value: flutamide
attributes.tag: Experimental Factor: dose
attributes.value: 10