title: 1G-4a
design: 2-10 ng of starting input genomic DNA was digested with 30 units of MspI (NEB). Fragments were ligated to pre-annealed adapters containing 5-methyl-cytosine instead of cytosine according to Illuminas specified guidelines. Adaptor-ligated fragments 50 bp in size were recovered using the DNA Clean & ConcentratorTM-5 (Cat#: D4003). The fragments were then bisulfite-treated using the EZ DNA Methylation-LightningTM Kit (Cat#: D5030). Preparative-scale PCR was performed and the resulting products were purified with DNA Clean & ConcentratorTM-5 (Cat#: D4003) for sequencing on an Illumina HiSeq.