title: Sequencing of SARS-CoV-2: NRW-31 without PCR, with 20 established SARS-CoV-2 primers
design: Viral RNA was reverse-transcribed to single-strand cDNA using 20 of the Artic network SARS-CoV-2 V1 primers. The used Primers were nCoV-2019-3-RIGHT, nCoV-2019-9-RIGHT, nCoV-2019-15-RIGHT, nCoV-2019-21-RIGHT, nCoV-2019-27-RIGHT, nCoV-2019-33-RIGHT, nCoV-2019-39-RIGHT, nCoV-2019-45-RIGHT, nCoV-2019-52-RIGHT, nCoV-2019-56-RIGHT, nCoV-2019-60-RIGHT, nCoV-2019-64-RIGHT, nCoV-2019-68-RIGHT, nCoV-2019-72-RIGHT, nCoV-2019-76-RIGHT, nCoV-2019-80-RIGHT, nCoV-2019-84-RIGHT, nCoV-2019-88-RIGHT, nCoV-2019-92-RIGHT, nCoV-2019-96-RIGHT, nCoV-2019-98-RIGHT. The cDNA was sequenced without prior PCR.