title: Rhodococcus corallinus strain B-276 whole genome shotgun (NSC)
library_construction_protocol: DNA extraction and shotgun pyrosequencing: Bacterial genomic DNA was extracted using a DNA extraction kit, ISOPLANT I (Nippon Gene, Tokyo, Japan). After extraction, the DNA was quantitatively and qualitatively analyzed by a Nanodrop (Thermo Fisher Scientific, Wilmington, DE, USA) and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). NSC and SC DNA samples were subjected to shotgun pyrosequencing by using a 454 GS Junior titanium chemistry (454 Life Sciences-a Roche Company, Branford, CT, USA), and DNA libraries were thus prepared. Pyrosequencing runs were carried out in duplicate for the NSC library and once for SC library. All operations were carried out according to the protocols provided by the manufacturers.