title: GSM2899175: soma_l(3)mbt_mut1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Somatic ovaries: 60-70 ovaries from females of maternal tud[1]/tud[B42] and zygotic l(3)mbt[GM76]/+ (control) or l(3)mbt[GM76]/Df genotypes were dissected in cold PBS and RNA was extracted using TRIzol (Invitrogen) following the manufacturer's protocol. Libraries were generated from 1µg of total RNA using the NEBNext Poly(A) magnetic Isolation Module (NEB #7490) and the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB # E7420). Libraries from 3 biological replicates were sequenced on an Illumina Hi-Seq2000, paired-end 50 run.
attributes.tag: GEO Accession
attributes.value: GSM2899175