title: GSM3145322: D4; Sus scrofa; RNA-Seq
library_construction_protocol: Adipocytes at different differentiation stages were subjected to total RNA extraction using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Total RNA from D0, D2, D4, and D8 (3 individuals/group) was pooled in equal amounts to generate mixed sample respectively. Sequencing libraries were prepared using NEB Next Ultra Directional RNA LibraryPrep Kit (NEB, Ispawich, MA, USA). Libraries were prepared for sequencing using Illumina Solexa transcriptome sequencing protocol after removing ribosomal RNA
attributes.tag: GEO Accession
attributes.value: GSM3145322