title: GSM4059335: at-microspore.sRNA.WT.replicate2; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: Total RNA was treated with DNAseI (Thermofischer: cat-EN0521) and then loaded on a 15% TBE-Urea polyacrylamide gel. RNA with a size of 15-27-nt was retrieved and eluted by crushing the gel in PAGE elution buffer (1M Tris pH7.5, 2.5M NaOAc, 0.5M EDTA pH8) followed by an overnight incubation and a new TRizoL extraction. sRNA seq libraries were generated with the NEBNext® Multiplex Small RNA kit (NEB #E7300S).
attributes.tag: GEO Accession
attributes.value: GSM4059335