title: GSM3983658: sgVector; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA is processed by mRNA enrichment or rRNA removal (mRNA enrichment: mRNA with polyA tail enriched with magnetic beads with OligodT; rRNA removal: hybridization of rRNA with DNA probe, selective digestion of DNA/RNA with RNaseH The hybridization strand is digested with DNaseI, and the desired RNA is obtained after purification.). The obtained mRNA is fragmented by adding an appropriate amount of the interrupting reagent under high temperature conditions, and the stranded cDNA is synthesized by using the broken mRNA as a template, and then the two-stranded cDNA is synthesized by synthesizing the two-stranded synthesis reaction system, and is purified and recovered by using a kit. The 3' end of the cDNA is ligated with the base "A" and ligated to the linker, and then the fragment size is selected and finally amplified by using PCR. The constructed library was examined by Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System and sequenced after passing the test.
attributes.tag: GEO Accession
attributes.value: GSM3983658