title: Illumina HiSeq 2500 sequencing; Sox18 ChIP-seq in HUVECs
design: Sox18 ChIP-seq in HUVECs
library_construction_protocol: HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2. Transfection was performed using Roche X-tremegene DNA transfection reagent in low (0.4%) FBS  Following IP, DNA amplification was performed using TruSeq ChIPseq kit (Illumina, IP-202-1012). The PCR product was purified using AMPure beads. The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KK4824)
attributes.tag: Experimental Factor: anti-cMyc (Cell Signaling, #2276)
attributes.value: immunoprecipitate
attributes.tag: Experimental Factor: transfected with cMyc
attributes.value: genotype