title: Microbial Community via 16S rRNA gene  sequencing for sample 13, technical replicate 1, on fish fillet of  Oncorhynchus nerka
design: Primers were used to target the 16S rRNA gene V3 and V4 region: Forward primer: 5' CCTACGGGNGGCWGCAG and Reverse primer: 5 GACTACHVGGGTATCTAATCC (Klindworth et al., 2013). The primer sequences were modified to include the Illumina overhang adapter sequences (Illumina, 2013).