title: All organs with library normalization
design: Total RNA was isolated from 11 organs (testis, ovary, male and female brain, spleen, liver, muscle, head and tail kidney, blood cells, gills) of multiple Asian seabass individuals using the RNeasy Mini Kit (Qiagen, Germany). RNA integrity and size distribution were checked with Bioanalyzer 2100 (Agilent Technologies, USA). The total RNA from the different organs were combined into a single pool for library construction, applying poly-A extraction, DSN-normalization (Evrogen, Russia) and the Epicentre ScriptSeq™ v2 strand specificity protocol (Epicentre Biotechnologies, USA). Sequencing was performed on the Illumina HiSeq 2000 (Illumina, Inc., USA) to generate 2x100bp paired-end reads.