title: GSM2476148: D-2 ESC +Dox; Mus musculus; Bisulfite-Seq
library_construction_protocol: generation protocol: D-2 ESCs were induced with doxycyclin for 3 passages. Genomic DNA was collected using phenol/chloroform extraction RRBS libraries were prepared using a commercial kit (Ovation RRBS Methyl-Seq System, NuGen, San Carlos, CA) following the manufacturer's protocol except that we pooled 12 individually barcoded reactions after the final repair step and performed the bisulfite conversion and library amplification as a pool. Libraries were sequenced on an Illumina HiSeq2500 high-output flowcell without a PhiX spike-in using Nugen's custom sequencing primer for read 1 (36 bases) and standard Illumina sequencing primers to read the 8-base sample barcodes.
attributes.tag: GEO Accession
attributes.value: GSM2476148