title: GSM2827654: control eutopic endometrium lncRNA, patient 1; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was extracted using a single-step acid guanidinium thiocyanate-phenol-chloroform method. The quality and purity of RNA were examined by a Nanodrop 8000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). RNA integrity was analyzed using the RNA 6000 Nano Kit and Small RNA Kit with the and a Bioanalyzer 2100 (Agilent, Santa Clara, CA). The samples with the absorbance wavelength ratio (A260/A280) ≥1.9 and a the RNA integrity number ≥ 8 were included. For small RNA sequencing, 3 μg of total RNA was used for library preparation.  3 μg of RNA per sample was prepared for library preparation. The Ribo-Zero Gold Kit (Epicentre, USA) and NEB Next Ultra RNA Library Prep Kit (NEB, Ispawich, USA) were used for rRNA removal and library construction following the manufacturer's protocols. For high-throughput sequencing, paired-end 150-bp sequencing of the cDNAs was performed on an Illumina HiSeq 4000 system (Illumina, USA) conducted by Annoroad Genome (Beijing, China).
attributes.tag: GEO Accession
attributes.value: GSM2827654