title: RNA-Seq of Bos taurus: Eyelids pigmentation
design: RNA extraction was performed using Trizol reagent, and quality of the obtained RNA was determined using a nanodrop spectrophotometer (OD260/OD280) to determine purity, as well as an agarose gel electrophoresis to observe potential RNA degradation and contamination. The RNA integrity was assessed using an Agilent Bioanalyzer, with RNA integrity number (RIN) values between 8 and 10. ). A total of 4 g of RNA per sample was used as the input for mRNA library preparations. Libraries were generated using Illumina Truseq Stranded mRNA kit.