title: GSM4670178: GFAP-ARO-KO_GCI_1 [GF_KO_GCI_22]; Mus musculus; RNA-Seq
library_construction_protocol: An astrocyte-specific aromatase knockout (GFAP-ARO-KO) mouse model was generated and utilized in this study. To conditionally delete locally synthesized aromatase in astrocyes in the brain, we generated Cyp19a1 Cre/LoxP conditional KO mice, under the control of the glial fibrillary acidic protein (GFAP) promoter, which expresses Cre exclusively in astrocytes. Ovariectomized female FLOX and GFAP-ARO-KO mice were subjected to two-vessel occlusion GCI and reperfusion for 24 hours. Bilateral ovariectomy of female mice was conducted one week prior to GCI surgery. For GCI, mice were anesthetized under isoflurane, and both common carotid arteries (CCAs) were isolated and occluded with clips for 20 minutes. Blood flow through the arteries was verified when the clips were removed. Total RNA was isolated from the hippocampi and subjected to RNA-seq analysis. Total RNA samples about 1-2 micro grams were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.
attributes.tag: GEO Accession
attributes.value: GSM4670178