title: RNA-seq of Pangasianodon hypophthalmus: adult heart
design: Total RNA was extracted from heart tissue of striped catfish using Trizol reagent (Invitrogen). Then, cDNA library was constructed using TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the manufacturer s procedure. The cDNA library was sequenced using Illumina Hiseq 2500 sequencing platform.