title: GSM4059245: Tf2_Cerebellum_4_E18.5; Mus musculus; Bisulfite-Seq
library_construction_protocol: Brains were excised and cerebellum, cortex and hippocampus were isolated. Genomic DNA was extracted with phenol/chloroform. 500ng of genomic DNA were bisulfite-converted using MethylEdge Bisulfite Conversion Kit (Promega). Targetted Bisultite-seq. Library was prepared as follows: briefly genomic DNA was bisulfite-converted and the amplified using specific primers: each organ in each developmental stage was associated to a distinct couple of Forw and Rev primers 5'-end tagged,  in order to be unambiguously identified in the sequencing analysis step. All the L1MdTf and IAPLTR1a amplicons were then pooled in equimolar quantities to obtain a final pooling concentration of 2 ng/μL. Library for DNA sequencing was produced on the pooled PCR products.
attributes.tag: GEO Accession
attributes.value: GSM4059245