title: GSM3004787: KO2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from the pancreas of three 3 weeks old male wild type (C57Bl6/N) and OCaR1–/– mice, respectively, using TRIZOL reagent (Thermo Fisher Scientific, Germany). The integrity of the RNA samples was verified using a 2100 Bioanalyzer system (Agilent Technologies, USA) and the RNA integrity number (RIN) was between 8.20 and 9.10. A cDNA library was generated using the NEB Next RNA Master Mix Library Prep Kit for Illumina with the NEB Next Oligodexoxynucleotides for Illumina (New England Biolabs, Germany). First strand synthesis was conducted employing 2.5 μg RNA that was polyA-RNA selected. The read length of the RNAseq (Hiseq2000 machine using the standard illumina sequencing by synthesis chemistry) was single read 50 base pair reads, SE.
attributes.tag: GEO Accession
attributes.value: GSM3004787