title: NextSeq 500 paired end sequencing; Altered expression of maize PLASTOCHRON1 enhances biomass and seed yield by extending cell division duration
design: Altered expression of maize PLASTOCHRON1 enhances biomass and seed yield by extending cell division duration
library_construction_protocol: Plants for leaf growth monitoring were grown under growth chamber conditions with controlled relative humidity (55%), temperature (24°C day/18°C night), and light intensity (170 mmol per m2 per second photosynthetic active radiation at plant level) provided by a combination of high-pressure sodium vapor (RNP-T/LR/400W/S/230/E40; Radium) and metal halide lamps with quartz burners (HRI-BT/400W/D230/E40; Radium) in a 16h/8h (day/night) cycle. Total RNA was isolated with the guanidinium thiocyanate-phenol-chloroform extraction method using TRI-reagent (Sigma-Aldrich) Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, poly(A)-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using a 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina), followed by sequencing on an Illumina HiSeq2000 with the TruSeq SBS Kit v3-HS (Illumina).
attributes.tag: Experimental Factor: wild type genotype
attributes.value: genotype
attributes.tag: Experimental Factor: 2
attributes.value: age