title: GSM4491377: HEK293T-a6A-IP mRNA; Homo sapiens; OTHER
library_construction_protocol: Total RNA was extracted with Trizol Reagent (Invitrogen), mRNA was extracted with GenElute™ Direct mRNA Miniprep Kits (Sigma), IP and cyclized products were precipitated with isopropanol. Libraries were constructed following Illumina's instructions except using HIV/RevertAid Reverse Transcriptase for reverse transcription in a6A-associated sequencing, Superscript II was used for m6A-seq of HEK293T cells and RNA-seq of HeLa cells.
attributes.tag: GEO Accession
attributes.value: GSM4491377