title: GSM4555309: CBP_naive1; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Heads were collected and homogenized in crosslinking buffer. The nuclei were dissolved in extraction buffer, sonicated to dissociate the individual nuclei, and precipitated with ANTI-FLAG M2 Affinity Gel. The precipitates were rinsed 4 times in extraction buffer, with 5 min nutation at 4℃ between washes, and subjected to ChIP analysis or RNA extraction for RNA-seq. The library for ChIP-seq was prepared by NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs inc. Ipswich, MA, USA), and that for nuclear RNA-seq was prepared by Oligo d(T)25 Magnetic Beads (New England Biolabs inc. Ipswich, MA, USA), and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs inc. Ipswich, MA, USA), according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4555309