title: RNA-seq of Pangasianodon hypophthalmus: egg ready for hatching
design: Total RNA was extracted from egg ready for hatching of striped catfish using Trizol reagent (Invitrogen). Then, cDNA library was constructed using TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the manufacturer s procedure. The cDNA library was sequenced using Illumina Hiseq 2500 sequencing platform