title: GSM3983556: WSB liver 4 weeks (31L); Mus musculus domesticus; RNA-Seq
library_construction_protocol: Organs were removed, snap frozen in liquid nitrogen, and stored at −80° C until RNA extraction. Liver RNA was harvested using Trizol reagent; adipose and hypothalmus RNA was harvested using the Qiagen RNeasy Lipid Tissue Mini Kit (74804). 1 μg of DNase-treated and purified total RNA for each sample was used for RNA-seq library preparation and construction via Illumina TruSeq RNA Sample Prep kits (v2, sets A and B; RS-122-2001 and RS-122-2202). Paired-end, 51-base pair RNA-seq libraries were generated using Illumina TruSeq RNA Sample Prep kits (v2, sets A and B; RS-122-2001 and RS-122-2202), TruSeq Rapid Cluster-HS kits (PE-402-4001), and Illumina TruSeq Rapid SBS kits (FC-402-4002).
attributes.tag: GEO Accession
attributes.value: GSM3983556