title: GSM4420142: PS2; Sus scrofa; RNA-Seq
library_construction_protocol: Total RNA was isolated from uninfected or infected ST cells using SuPerfecTRITM Total RNA Isolation Reagent (Pufei) according to the manufacturer. A total amount of 5 µg RNA per sample was used as input material for RNA sample preparation. Firstly, ribosomal RNAs were depleted by using Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA) to get rRNA-depleted RNAs. rRNA-depleted RNAs were further treated with RNase R (Epicentre, USA) and then were subjected to Trizol extraction. Subsequently, sequencing libraries were generated using the rRNA-depleted and RNase R digested RNAs by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
attributes.tag: GEO Accession
attributes.value: GSM4420142