title: Nothofagus_dombeyi_litter_fungi_control    
design: The ITS1-ITS2 region was amplified with ITS1-Fw (5Õ-CTTGGTCATTTAGAGGAAGTAA-3Õ) and ITS2-Rv (5Õ-GCTGCGTTCTTCATCGATGC-3Õ) primers fused with 454 adapters within the same primer. Each sample correspond to a pool of five independent samples, each amplified by duplicate PCR reactions to reduce sample and PCR biases, respectively. All amplicons were cleaned using Ampure DNA capture beads (Agencourt- Beckman Coulter, Inc.) and pooled in equimolar concentrations before sequencing on a Genome Sequencer FLX (454-Roche Applied Sciences) using Titanium Chemistry according to the manufacturerÕs instructions.