title: 16S rRNA of rhizosphere soil sample:S. breweri
design: PCR was carried out in 25 l reactions including 1 l genomic DNA, 0.5 l of each 10 M primer, 12.5 l of MyTaq Hot Start Red Mix (Bioline), and 10.5 l of dH2O. PCR reactions were set up on ice to minimize non-specific amplification and primer dimerization. PCR conditions were: denaturation at 94C for 2 min; 34 amplification cycles of 30 sec at 94C, 30 sec at 51C and 30 sec at 72C; followed by a 10 sec final extension at 72C.