title: Specific-locus amplified fragment sequencing (SLAF-seq) of a segregating Agropyron F1 population derived from an interspecific cross between two cross-pollinated diploid collections Agropyron cristatum (L.) Beauv.'Z1842' and Agropyron mongolicum Keng'Z2098'
design: SLAF-seq was used to genotype a total of 133 individuals and their two parents. Genomic DNA from each sample was digested with NlaIII/MseI (NEB), and then treated with T4 DNAligase (NEB), ATP (NEB), and MseI adapter at 37°C. The reaction solutions were diluted and mixed with dNTP, Taq DNA polymerase (NEB) and MseI primer containing barcode 1 to carry out PCR amplification. The E.Z.N.A. Cycle Pure Kit (Omega, London, UK) was used to purify the PCR products. The purified PCR products were pooled and incubated at 37°C with MseI, T4 DNA ligase, ATP, and Solexa adapter. The reaction products were purified using a Quick Spincolumn (Qiagen) and electrophoresed in a 2% agarose gel. Fragments of 280–330bp (including adaptersequence indexes and adaptors) were isolated using Gel Extraction Kits (Qiagen) and then subjected to PCR with Phusion Master Mix (NEB) and Solexa amplification primer mix to add barcode 2. PCR products were purified for paired-end sequencing on an Illumina Hiseq2000 sequencing platform. Sequence_file_name,Sample_ID,Sample_description  Agropyron_M_1.fq.gz,Z2098,"female parent, A. mongolicum Keng，2n=2x=14，PP genome"  Agropyron_M_2.fq.gz,Z2098,"female parent, A. mongolicum Keng，2n=2x=14，PP genome"  Agropyron_P_1.fq.gz,Z1842,"male parent, A. cristatum (L.) Beauv，2n=2x=14，PP genome"  Agropyron_P_2.fq.gz,Z1842,"male parent, A. cristatum (L.) Beauv，2n=2x=14，PP genome"  Agropyron_ab_1.fq.gz,1,F1 hybrid between Z1842 and Z2098  Agropyron_ab_2.fq.gz,1,F1 hybrid between Z1842 and Z2098  Agropyron_ac_1.fq.gz,2,F1 hybrid between Z1842 and Z2098  Agropyron_ac_2.fq.gz,2,F1 hybrid between Z1842 and Z2098  Agropyron_ad_1.fq.gz,3,F1 hybrid between Z1842 and Z2098  Agropyron_ad_2.fq.gz,3,F1 hybrid between Z1842 and Z2098  Agropyron_ae_1.fq.gz,4,F1 hybrid between Z1842 and Z2098  Agropyron_ae_2.fq.gz,4,F1 hybrid between Z1842 and Z2098  Agropyron_af_1.fq.gz,5,F1 hybrid between Z1842 and Z2098  Agropyron_af_2.fq.gz,5,F1 hybrid between Z1842 and Z2098  Agropyron_ag_1.fq.gz,6,F1 hybrid between Z1842 and Z2098  Agropyron_ag_2.fq.gz,6,F1 hybrid between Z1842 and Z2098  Agropyron_ah_1.fq.gz,7,F1 hybrid between Z1842 and Z2098  Agropyron_ah_2.fq.gz,7,F1 hybrid between Z1842 and Z2098  Agropyron_ai_1.fq.gz,8,F1 hybrid between Z1842 and Z2098  Agropyron_ai_2.fq.gz,8,F1 hybrid between Z1842 and Z2098  Agropyron_aj_1.fq.gz,9,F1 hybrid between Z1842 and Z2098  Agropyron_aj_2.fq.gz,9,F1 hybrid between Z1842 and Z2098  Agropyron_ak_1.fq.gz,10,F1 hybrid between Z1842 and Z2098  Agropyron_ak_2.fq.gz,10,F1 hybrid between Z1842 and Z2098  Agropyron_al_1.fq.gz,11,F1 hybrid between Z1842 and Z2098  Agropyron_al_2.fq.gz,11,F1 hybrid between Z1842 and Z2098  Agropyron_am_1.fq.gz,12,F1 hybrid between Z1842 and Z2098  Agropyron_am_2.fq.gz,12,F1 hybrid between Z1842 and Z2098  Agropyron_an_1.fq.gz,13,F1 hybrid between Z1842 and Z2098  Agropyron_an_2.fq.gz,13,F1 hybrid between Z1842 and Z2098  Agropyron_ao_1.fq.gz,14,F1 hybrid between Z1842 and Z2098  Agropyron_ao_2.fq.gz,14,F1 hybrid between Z1842 and Z2098  Agropyron_ap_1.fq.gz,15,F1 hybrid between Z1842 and Z2098  Agropyron_ap_2.fq.gz,15,F1 hybrid between Z1842 and Z2098  Agropyron_aq_1.fq.gz,16,F1 hybrid between Z1842 and Z2098  Agropyron_aq_2.fq.gz,16,F1 hybrid between Z1842 and Z2098  Agropyron_ar_1.fq.gz,17,F1 hybrid between Z1842 and Z2098  Agropyron_ar_2.fq.gz,17,F1 hybrid between Z1842 and Z2098  Agropyron_as_1.fq.gz,18,F1 hybrid between Z1842 and Z2098  Agropyron_as_2.fq.gz,18,F1 hybrid between Z1842 and Z2098  Agropyron_at_1.fq.gz,19,F1 hybrid between Z1842 and Z2098  Agropyron_at_2.fq.gz,19,F1 hybrid between Z1842 and Z2098  Agropyron_au_1.fq.gz,20,F1 hybrid between Z1842 and Z2098  Agropyron_au_2.fq.gz,20,F1 hybrid between Z1842 and Z2098  Agropyron_av_1.fq.gz,21,F1 hybrid between Z1842 and Z2098  Agropyron_av_2.fq.gz,21,F1 hybrid between Z1842 and Z2098  Agropyron_aw_1.fq.gz,22,F1 hybrid between Z1842 and Z2098  Agropyron_aw_2.fq.gz,22,F1 hybrid between Z1842 and Z2098  Agropyron_ay_1.fq.gz,23,F1 hybrid between Z1842 and Z2098  Agropyron_ay_2.fq.gz,23,F1 hybrid between Z1842 and Z2098  Agropyron_ba_1.fq.gz,24,F1 hybrid between Z1842 and Z2098  Agropyron_ba_2.fq.gz,24,F1 hybrid between Z1842 and Z2098  Agropyron_bb_1.fq.gz,25,F1 hybrid between Z1842 and Z2098  Agropyron_bb_2.fq.gz,25,F1 hybrid between Z1842 and Z2098  Agropyron_bc_1.fq.gz,26,F1 hybrid between Z1842 and Z2098  Agropyron_bc_2.fq.gz,26,F1 hybrid between Z1842 and Z2098  Agropyron_bf_1.fq.gz,27,F1 hybrid between Z1842 and Z2098  Agropyron_bf_2.fq.gz,27,F1 hybrid between Z1842 and Z2098  Agropyron_bg_1.fq.gz,28,F1 hybrid between Z1842 and Z2098  Agropyron_bg_2.fq.gz,28,F1 hybrid between Z1842 and Z2098  Agropyron_bh_1.fq.gz,29,F1 hybrid between Z1842 and Z2098  Agropyron_bh_2.fq.gz,29,F1 hybrid between Z1842 and Z2098  Agropyron_bi_1.fq.gz,30,F1 hybrid between Z1842 and Z2098  Agropyron_bi_2.fq.gz,30,F1 hybrid between Z1842 and Z2098  Agropyron_bj_1.fq.gz,31,F1 hybrid between Z1842 and Z2098  Agropyron_bj_2.fq.gz,31,F1 hybrid between Z1842 and Z2098  Agropyron_bk_1.fq.gz,32,F1 hybrid between Z1842 and Z2098  Agropyron_bk_2.fq.gz,32,F1 hybrid between Z1842 and Z2098  Agropyron_bm_1.fq.gz,33,F1 hybrid between Z1842 and Z2098  Agropyron_bm_2.fq.gz,33,F1 hybrid between Z1842 and Z2098  Agropyron_bn_1.fq.gz,34,F1 hybrid between Z1842 and Z2098  Agropyron_bn_2.fq.gz,34,F1 hybrid between Z1842 and Z2098  Agropyron_bo_1.fq.gz,35,F1 hybrid between Z1842 and Z2098  Agropyron_bo_2.fq.gz,35,F1 hybrid between Z1842 and Z2098  Agropyron_bq_1.fq.gz,36,F1 hybrid between Z1842 and Z2098  Agropyron_bq_2.fq.gz,36,F1 hybrid between Z1842 and Z2098  Agropyron_br_1.fq.gz,37,F1 hybrid between Z1842 and Z2098  Agropyron_br_2.fq.gz,37,F1 hybrid between Z1842 and Z2098  Agropyron_bs_1.fq.gz,38,F1 hybrid between Z1842 and Z2098  Agropyron_bs_2.fq.gz,38,F1 hybrid between Z1842 and Z2098  Agropyron_bt_1.fq.gz,39,F1 hybrid between Z1842 and Z2098  Agropyron_bt_2.fq.gz,39,F1 hybrid between Z1842 and Z2098  Agropyron_bu_1.fq.gz,40,F1 hybrid between Z1842 and Z2098  Agropyron_bu_2.fq.gz,40,F1 hybrid between Z1842 and Z2098  Agropyron_bv_1.fq.gz,41,F1 hybrid between Z1842 and Z2098  Agropyron_bv_2.fq.gz,41,F1 hybrid between Z1842 and Z2098  Agropyron_bw_1.fq.gz,42,F1 hybrid between Z1842 and Z2098  Agropyron_bw_2.fq.gz,42,F1 hybrid between Z1842 and Z2098  Agropyron_bx_1.fq.gz,43,F1 hybrid between Z1842 and Z2098  Agropyron_bx_2.fq.gz,43,F1 hybrid between Z1842 and Z2098  Agropyron_by_1.fq.gz,44,F1 hybrid between Z1842 and Z2098  Agropyron_by_2.fq.gz,44,F1 hybrid between Z1842 and Z2098  Agropyron_bz_1.fq.gz,45,F1 hybrid between Z1842 and Z2098  Agropyron_bz_2.fq.gz,45,F1 hybrid between Z1842 and Z2098  Agropyron_ca_1.fq.gz,46,F1 hybrid between Z1842 and Z2098  Agropyron_ca_2.fq.gz,46,F1 hybrid between Z1842 and Z2098  Agropyron_cb_1.fq.gz,47,F1 hybrid between Z1842 and Z2098  Agropyron_cb_2.fq.gz,47,F1 hybrid between Z1842 and Z2098  Agropyron_cc_1.fq.gz,48,F1 hybrid between Z1842 and Z2098  Agropyron_cc_2.fq.gz,48,F1 hybrid between Z1842 and Z2098  Agropyron_cd_1.fq.gz,49,F1 hybrid between Z1842 and Z2098  Agropyron_cd_2.fq.gz,49,F1 hybrid between Z1842 and Z2098  Agropyron_ce_1.fq.gz,50,F1 hybrid between Z1842 and Z2098  Agropyron_ce_2.fq.gz,50,F1 hybrid between Z1842 and Z2098  Agropyron_cf_1.fq.gz,51,F1 hybrid between Z1842 and Z2098  Agropyron_cf_2.fq.gz,51,F1 hybrid between Z1842 and Z2098  Agropyron_cg_1.fq.gz,52,F1 hybrid between Z1842 and Z2098  Agropyron_cg_2.fq.gz,52,F1 hybrid between Z1842 and Z2098  Agropyron_ci_1.fq.gz,53,F1 hybrid between Z1842 and Z2098  Agropyron_ci_2.fq.gz,53,F1 hybrid between Z1842 and Z2098  Agropyron_cj_1.fq.gz,54,F1 hybrid between Z1842 and Z2098  Agropyron_cj_2.fq.gz,54,F1 hybrid between Z1842 and Z2098  Agropyron_ck_1.fq.gz,55,F1 hybrid between Z1842 and Z2098  Agropyron_ck_2.fq.gz,55,F1 hybrid between Z1842 and Z2098  Agropyron_cm_1.fq.gz,56,F1 hybrid between Z1842 and Z2098  Agropyron_cm_2.fq.gz,56,F1 hybrid between Z1842 and Z2098  Agropyron_cn_1.fq.gz,57,F1 hybrid between Z1842 and Z2098  Agropyron_cn_2.fq.gz,57,F1 hybrid between Z1842 and Z2098  Agropyron_co_1.fq.gz,58,F1 hybrid between Z1842 and Z2098  Agropyron_co_2.fq.gz,58,F1 hybrid between Z1842 and Z2098  Agropyron_cq_1.fq.gz,59,F1 hybrid between Z1842 and Z2098  Agropyron_cq_2.fq.gz,59,F1 hybrid between Z1842 and Z2098  Agropyron_cr_1.fq.gz,60,F1 hybrid between Z1842 and Z2098  Agropyron_cr_2.fq.gz,60,F1 hybrid between Z1842 and Z2098  Agropyron_cs_1.fq.gz,61,F1 hybrid between Z1842 and Z2098  Agropyron_cs_2.fq.gz,61,F1 hybrid between Z1842 and Z2098  Agropyron_ct_1.fq.gz,62,F1 hybrid between Z1842 and Z2098  Agropyron_ct_2.fq.gz,62,F1 hybrid between Z1842 and Z2098  Agropyron_cu_1.fq.gz,63,F1 hybrid between Z1842 and Z2098  Agropyron_cu_2.fq.gz,63,F1 hybrid between Z1842 and Z2098  Agropyron_cv_1.fq.gz,64,F1 hybrid between Z1842 and Z2098  Agropyron_cv_2.fq.gz,64,F1 hybrid between Z1842 and Z2098  Agropyron_cw_1.fq.gz,65,F1 hybrid between Z1842 and Z2098  Agropyron_cw_2.fq.gz,65,F1 hybrid between Z1842 and Z2098  Agropyron_cx_1.fq.gz,66,F1 hybrid between Z1842 and Z2098  Agropyron_cx_2.fq.gz,66,F1 hybrid between Z1842 and Z2098  Agropyron_cy_1.fq.gz,67,F1 hybrid between Z1842 and Z2098  Agropyron_cy_2.fq.gz,67,F1 hybrid between Z1842 and Z2098  Agropyron_cz_1.fq.gz,68,F1 hybrid between Z1842 and Z2098  Agropyron_cz_2.fq.gz,68,F1 hybrid between Z1842 and Z2098  Agropyron_da_1.fq.gz,69,F1 hybrid between Z1842 and Z2098  Agropyron_da_2.fq.gz,69,F1 hybrid between Z1842 and Z2098  Agropyron_db_1.fq.gz,70,F1 hybrid between Z1842 and Z2098  Agropyron_db_2.fq.gz,70,F1 hybrid between Z1842 and Z2098  Agropyron_dc_1.fq.gz,71,F1 hybrid between Z1842 and Z2098  Agropyron_dc_2.fq.gz,71,F1 hybrid between Z1842 and Z2098  Agropyron_dd_1.fq.gz,72,F1 hybrid between Z1842 and Z2098  Agropyron_dd_2.fq.gz,72,F1 hybrid between Z1842 and Z2098  Agropyron_de_1.fq.gz,73,F1 hybrid between Z1842 and Z2098  Agropyron_de_2.fq.gz,73,F1 hybrid between Z1842 and Z2098  Agropyron_df_1.fq.gz,74,F1 hybrid between Z1842 and Z2098  Agropyron_df_2.fq.gz,74,F1 hybrid between Z1842 and Z2098  Agropyron_dg_1.fq.gz,75,F1 hybrid between Z1842 and Z2098  Agropyron_dg_2.fq.gz,75,F1 hybrid between Z1842 and Z2098  Agropyron_dh_1.fq.gz,76,F1 hybrid between Z1842 and Z2098  Agropyron_dh_2.fq.gz,76,F1 hybrid between Z1842 and Z2098  Agropyron_di_1.fq.gz,77,F1 hybrid between Z1842 and Z2098  Agropyron_di_2.fq.gz,77,F1 hybrid between Z1842 and Z2098  Agropyron_dj_1.fq.gz,78,F1 hybrid between Z1842 and Z2098  Agropyron_dj_2.fq.gz,78,F1 hybrid between Z1842 and Z2098  Agropyron_dk_1.fq.gz,79,F1 hybrid between Z1842 and Z2098  Agropyron_dk_2.fq.gz,79,F1 hybrid between Z1842 and Z2098  Agropyron_dl_1.fq.gz,80,F1 hybrid between Z1842 and Z2098  Agropyron_dl_2.fq.gz,80,F1 hybrid between Z1842 and Z2098  Agropyron_dn_1.fq.gz,81,F1 hybrid between Z1842 and Z2098  Agropyron_dn_2.fq.gz,81,F1 hybrid between Z1842 and Z2098  Agropyron_dp_1.fq.gz,82,F1 hybrid between Z1842 and Z2098  Agropyron_dp_2.fq.gz,82,F1 hybrid between Z1842 and Z2098  Agropyron_dq_1.fq.gz,83,F1 hybrid between Z1842 and Z2098  Agropyron_dq_2.fq.gz,83,F1 hybrid between Z1842 and Z2098  Agropyron_dr_1.fq.gz,84,F1 hybrid between Z1842 and Z2098  Agropyron_dr_2.fq.gz,84,F1 hybrid between Z1842 and Z2098  Agropyron_ds_1.fq.gz,85,F1 hybrid between Z1842 and Z2098  Agropyron_ds_2.fq.gz,85,F1 hybrid between Z1842 and Z2098  Agropyron_du_1.fq.gz,86,F1 hybrid between Z1842 and Z2098  Agropyron_du_2.fq.gz,86,F1 hybrid between Z1842 and Z2098  Agropyron_dv_1.fq.gz,87,F1 hybrid between Z1842 and Z2098  Agropyron_dv_2.fq.gz,87,F1 hybrid between Z1842 and Z2098  Agropyron_dw_1.fq.gz,88,F1 hybrid between Z1842 and Z2098  Agropyron_dw_2.fq.gz,88,F1 hybrid between Z1842 and Z2098  Agropyron_dx_1.fq.gz,89,F1 hybrid between Z1842 and Z2098  Agropyron_dx_2.fq.gz,89,F1 hybrid between Z1842 and Z2098  Agropyron_dy_1.fq.gz,90,F1 hybrid between Z1842 and Z2098  Agropyron_dy_2.fq.gz,90,F1 hybrid between Z1842 and Z2098  Agropyron_dz_1.fq.gz,91,F1 hybrid between Z1842 and Z2098  Agropyron_dz_2.fq.gz,91,F1 hybrid between Z1842 and Z2098  Agropyron_eb_1.fq.gz,92,F1 hybrid between Z1842 and Z2098  Agropyron_eb_2.fq.gz,92,F1 hybrid between Z1842 and Z2098  Agropyron_ec_1.fq.gz,93,F1 hybrid between Z1842 and Z2098  Agropyron_ec_2.fq.gz,93,F1 hybrid between Z1842 and Z2098  Agropyron_ed_1.fq.gz,94,F1 hybrid between Z1842 and Z2098  Agropyron_ed_2.fq.gz,94,F1 hybrid between Z1842 and Z2098  Agropyron_ef_1.fq.gz,95,F1 hybrid between Z1842 and Z2098  Agropyron_ef_2.fq.gz,95,F1 hybrid between Z1842 and Z2098  Agropyron_ei_1.fq.gz,96,F1 hybrid between Z1842 and Z2098  Agropyron_ei_2.fq.gz,96,F1 hybrid between Z1842 and Z2098  Agropyron_ej_1.fq.gz,97,F1 hybrid between Z1842 and Z2098  Agropyron_ej_2.fq.gz,97,F1 hybrid between Z1842 and Z2098  Agropyron_ek_1.fq.gz,98,F1 hybrid between Z1842 and Z2098  Agropyron_ek_2.fq.gz,98,F1 hybrid between Z1842 and Z2098  Agropyron_el_1.fq.gz,99,F1 hybrid between Z1842 and Z2098  Agropyron_el_2.fq.gz,99,F1 hybrid between Z1842 and Z2098  Agropyron_em_1.fq.gz,100,F1 hybrid between Z1842 and Z2098  Agropyron_em_2.fq.gz,100,F1 hybrid between Z1842 and Z2098  Agropyron_en_1.fq.gz,101,F1 hybrid between Z1842 and Z2098  Agropyron_en_2.fq.gz,101,F1 hybrid between Z1842 and Z2098  Agropyron_eo_1.fq.gz,102,F1 hybrid between Z1842 and Z2098  Agropyron_eo_2.fq.gz,102,F1 hybrid between Z1842 and Z2098  Agropyron_ep_1.fq.gz,103,F1 hybrid between Z1842 and Z2098  Agropyron_ep_2.fq.gz,103,F1 hybrid between Z1842 and Z2098  Agropyron_eq_1.fq.gz,104,F1 hybrid between Z1842 and Z2098  Agropyron_eq_2.fq.gz,104,F1 hybrid between Z1842 and Z2098  Agropyron_er_1.fq.gz,105,F1 hybrid between Z1842 and Z2098  Agropyron_er_2.fq.gz,105,F1 hybrid between Z1842 and Z2098  Agropyron_es_1.fq.gz,106,F1 hybrid between Z1842 and Z2098  Agropyron_es_2.fq.gz,106,F1 hybrid between Z1842 and Z2098  Agropyron_et_1.fq.gz,107,F1 hybrid between Z1842 and Z2098  Agropyron_et_2.fq.gz,107,F1 hybrid between Z1842 and Z2098  Agropyron_eu_1.fq.gz,108,F1 hybrid between Z1842 and Z2098  Agropyron_eu_2.fq.gz,108,F1 hybrid between Z1842 and Z2098  Agropyron_ev_1.fq.gz,109,F1 hybrid between Z1842 and Z2098  Agropyron_ev_2.fq.gz,109,F1 hybrid between Z1842 and Z2098  Agropyron_ex_1.fq.gz,110,F1 hybrid between Z1842 and Z2098  Agropyron_ex_2.fq.gz,110,F1 hybrid between Z1842 and Z2098  Agropyron_ey_1.fq.gz,111,F1 hybrid between Z1842 and Z2098  Agropyron_ey_2.fq.gz,111,F1 hybrid between Z1842 and Z2098  Agropyron_fb_1.fq.gz,112,F1 hybrid between Z1842 and Z2098  Agropyron_fb_2.fq.gz,112,F1 hybrid between Z1842 and Z2098  Agropyron_fc_1.fq.gz,113,F1 hybrid between Z1842 and Z2098  Agropyron_fc_2.fq.gz,113,F1 hybrid between Z1842 and Z2098  Agropyron_aa_1.fq.gz,114,F1 hybrid between Z1842 and Z2098  Agropyron_aa_2.fq.gz,114,F1 hybrid between Z1842 and Z2098  Agropyron_ax_1.fq.gz,115,F1 hybrid between Z1842 and Z2098  Agropyron_ax_2.fq.gz,115,F1 hybrid between Z1842 and Z2098  Agropyron_az_1.fq.gz,116,F1 hybrid between Z1842 and Z2098  Agropyron_az_2.fq.gz,116,F1 hybrid between Z1842 and Z2098  Agropyron_bd_1.fq.gz,117,F1 hybrid between Z1842 and Z2098  Agropyron_bd_2.fq.gz,117,F1 hybrid between Z1842 and Z2098  Agropyron_be_1.fq.gz,118,F1 hybrid between Z1842 and Z2098  Agropyron_be_2.fq.gz,118,F1 hybrid between Z1842 and Z2098  Agropyron_bl_1.fq.gz,119,F1 hybrid between Z1842 and Z2098  Agropyron_bl_2.fq.gz,119,F1 hybrid between Z1842 and Z2098  Agropyron_bp_1.fq.gz,120,F1 hybrid between Z1842 and Z2098  Agropyron_bp_2.fq.gz,120,F1 hybrid between Z1842 and Z2098  Agropyron_ch_1.fq.gz,121,F1 hybrid between Z1842 and Z2098  Agropyron_ch_2.fq.gz,121,F1 hybrid between Z1842 and Z2098  Agropyron_cl_1.fq.gz,122,F1 hybrid between Z1842 and Z2098  Agropyron_cl_2.fq.gz,122,F1 hybrid between Z1842 and Z2098  Agropyron_cp_1.fq.gz,123,F1 hybrid between Z1842 and Z2098  Agropyron_cp_2.fq.gz,123,F1 hybrid between Z1842 and Z2098  Agropyron_dm_1.fq.gz,124,F1 hybrid between Z1842 and Z2098  Agropyron_dm_2.fq.gz,124,F1 hybrid between Z1842 and Z2098  Agropyron_do_1.fq.gz,125,F1 hybrid between Z1842 and Z2098  Agropyron_do_2.fq.gz,125,F1 hybrid between Z1842 and Z2098  Agropyron_dt_1.fq.gz,126,F1 hybrid between Z1842 and Z2098  Agropyron_dt_2.fq.gz,126,F1 hybrid between Z1842 and Z2098  Agropyron_ea_1.fq.gz,127,F1 hybrid between Z1842 and Z2098  Agropyron_ea_2.fq.gz,127,F1 hybrid between Z1842 and Z2098  Agropyron_ee_1.fq.gz,128,F1 hybrid between Z1842 and Z2098  Agropyron_ee_2.fq.gz,128,F1 hybrid between Z1842 and Z2098  Agropyron_eg_1.fq.gz,129,F1 hybrid between Z1842 and Z2098  Agropyron_eg_2.fq.gz,129,F1 hybrid between Z1842 and Z2098  Agropyron_eh_1.fq.gz,130,F1 hybrid between Z1842 and Z2098  Agropyron_eh_2.fq.gz,130,F1 hybrid between Z1842 and Z2098  Agropyron_ew_1.fq.gz,131,F1 hybrid between Z1842 and Z2098  Agropyron_ew_2.fq.gz,131,F1 hybrid between Z1842 and Z2098  Agropyron_ez_1.fq.gz,132,F1 hybrid between Z1842 and Z2098  Agropyron_ez_2.fq.gz,132,F1 hybrid between Z1842 and Z2098  Agropyron_fa_1.fq.gz,133,F1 hybrid between Z1842 and Z2098  Agropyron_fa_2.fq.gz,133,F1 hybrid between Z1842 and Z2098