title: RNA-seq of Providencia rettgeri HF16
design: For transcriptomic analysis, strain HF16 cultured untreated and treated with 10 mM Na2SeO3 for 12 h were harvested by gentle centrifugation (5000 g for 3 min, at 4C). After washing twice using icy deionized water, the total RNA of each sample was extracted using TRIzol Reagent (Invitrogen) and quantified by an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). Next-generation sequencing library preparations were constructed according to the manufacturers protocol (NEB Next Ultra Directional RNA Library Prep Kit for Illumina) and sequenced using the HiSeq-1006 platform (BGI, Wuhan, China) and paired-end reads were generated. This analysis was performed with three biological replicates.