title: Transcriptional and epigenetic profiling of the progression of hESCs to beta cells
design: Transcriptional and epigenetic profiling of the progression of hESCs to beta cells
library_construction_protocol: All ChIP-seq libraries were prepared as per Illuminas instructions (http://www.illumina.com). Briefly, total ChIP DNA was subjected to end-repair and ligated to the Adaptor Oligo Mix (Illumina). For input library preparation, 10 ng of input DNA from each sample was used. After adaptor ligation, DNA fragments were size-fractionated by gel electrophoresis and excised at (+25 bp,-25bp). Following gel purification, DNA fragments were amplified with 18 PCR cycles and purified using a MiniElute PCR Purification kit (Qiagen).
attributes.tag: Experimental Factor: TIMEPOINT
attributes.value: day 2
attributes.tag: Experimental Factor: CD antibody
attributes.value: n/a
attributes.tag: Experimental Factor: histone antibody
attributes.value: H3K27me3 Millipore 07-449  Lot#: DAM1588246
attributes.tag: Experimental Factor: CELL_TYPE
attributes.value: embryonic stem cell
attributes.tag: Experimental Factor: CELL PROPERTY
attributes.value: embryonic stem cell directed to definitive endoderm