title: GSM3004817: pancreatic_islets_15w_WT_H3K27me2; Mus musculus; ChIP-Seq
library_construction_protocol: NEXSON ChIP-seq workflow (Arrigoni et al., 2015) was applied to freshly isolated mouse islets Primary islets were obtained by collagenase digestion with reference to (Li et al., 2009). In brief, adult pancreata were perfused through the common bile duct using a 27/30-gauge needle with Collegenase IV (Sigma) solution. Perfused pancreata were digested and purified by centrifugation in Histopaque gradient (Sigma). Isolated islets were hand picked and cultured in complete media (RMPI-1640 containing 11mM glucose, 10% FBS, 0.1% Penincillin/Streptomycin) and maintained at 37°C in 5% CO2 environment.
attributes.tag: GEO Accession
attributes.value: GSM3004817