title: 1x151 bp RNA-Seq Metatranscriptome for J2-677-SSyellow hydrothermal vent Fe mat - T0 Fe addition time point
design: RNA expression fixed using RNA later at the indicated time points after Fe(II) amendment during an Fe-oxidation experiment. pTXB1 internal standard added prior to library prep. RNA-seq library prepared from total RNA using the Ovation RNA-seq system V2 kit, which included preferential priming of non-rRNA sequences. Single end 1x151 bp run. 26-multiplex.