title: GSM3082766: C. frutescens Fruit; Capsicum frutescens; miRNA-Seq
library_construction_protocol: The RNA Extraction was carried out using Macherey-Nagel NucleoSpin RNA kit (MN, US). The total RNA were pooled from the three biological replicates from the individual tissue samples. Further, the quality, and the quantity of RNAs was estimated with Bioanalyzer (Agilent) plant Nano kit. For small RNA library preparation, RNA samples with 260/280 ratio of 1.9–2.0 and RNA integrity number (RIN) of ≥ 8 were selected. The sRNA libraries were prepared by using TruSeq Small RNA sample preparation kit (Illumina, US). Each sRNA library was sequenced using Illumina Genome Analyser II at Genotypic Technologies Pvt. Ltd, Bangalore, India.
attributes.tag: GEO Accession
attributes.value: GSM3082766