title: nhl-2 20°C RNA-seq r1
design: Total RNA was extracted from triplicate samples of Bristol N2 (wild type) and nhl-2(ok818) mutants that were grown to gravid adulthood at either 20°C or 25°C on OP50 E. coli. mRNA-seq libraries were generated and sequenced at the Donnelly Sequencing Centre (University of Toronto) using the Illumina Tru-Seq Stranded mRNA Library Construction Kit and the HiSeq2500 sequencer.