title: GSM3004159: MYC-OE_3_130d (techn. Rep. 1); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from individual organoids using RNeasy Micro Kit (QIAGEN, cat. #74004) according to the manufacturers instructions. RNA concentration and integrity was analysed using RNA 6000 Nano Chip (Agilent techReplicatenologies, cat. #5067-1511). RNA was enriched for mRNA using SMART-Seq v4 Ultra Low Input RNA Kit  (Clontech, cat. # 634891). Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, cat. # E7420L). Barcoded samples were multiplexed and sequenced 50bp SE on a HighSeq2500 (Illumina). Sample preparation and sequencing was performed at the VBCF NGS Unit (www.vbcf.ac.at). Samples were sequenced twice each.
attributes.tag: GEO Accession
attributes.value: GSM3004159