title: GSM2827672: control eutopic endometrium miRNA, patient 6; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was extracted using a single-step acid guanidinium thiocyanate-phenol-chloroform method. The quality and purity of RNA were examined by a Nanodrop 8000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). RNA integrity was analyzed using the RNA 6000 Nano Kit and Small RNA Kit with the and a Bioanalyzer 2100 (Agilent, Santa Clara, CA). The samples with the absorbance wavelength ratio (A260/A280) ≥1.9 and a the RNA integrity number ≥ 8 were included. For small RNA sequencing, 3 μg of total RNA was used for library preparation. For mRNA sequencing, 3 μg of RNA per sample was prepared for library preparation. The purified libraries were sequenced on an Illumina HiSeq 2000 platform in Annoroad Genome (Beijing, China).
attributes.tag: GEO Accession
attributes.value: GSM2827672