title: MS MP MO SL RNA-Seq
design: MS MP MO SL RNA-Seq
library_construction_protocol: growth protocol: For collecting SL, seeds of Zheng 58 were surface-sterilized with 3?G sodium hypochlorite for 10 min and rinsed in distilled water. Sterilized seeds were pre-germinated on moistened pledget in a plant growth chamber at 25 C under 16/8 h light/dark cycle for about six days. Then we collected the whole seedling tissue as SL for RNA-seq analysis. For collecting the reproductive tissues including MS, MP, and MO, plants were grown in silt loam soil at the Shandong Agricultural University experimental station in Taian, China (35 58'N, 116 38'E, 150m above sea level). Before tassels shedding pollens and silk growing from husk, we bagged the tassel and husk of experimental plants to prevent the pollination from pathogen and other plants. In the second to fifth days after anther dehiscence, mature pollen grains (MP) were collected in 1.5 mL Eppendorf tubes during 8:00-10:00am. After silks had grown from the husk for approximately three days, the upper parts (about 4 cm) of the silks were harvested as the mature silk tissues. After removing glumes around and silk on the top,  whole mature ovaries distributed in a third part area in the middle of an ear were collected. nucleic acid extraction protocol:  Total RNA of MS was extracted by using TRIZOL reagent (Invitrogen, http://www.invitrogen.com) according to the manufacturers protocol, and the hexadecyl trimethyl ammonium bromide (CTAB) protocol was performed to extract the total RNA of MP, MO, and SL.
attributes.tag: Experimental Factor: ORGANISMPART
attributes.value: silk