title: GSM4555803: glioma stem-like cell line BTSC349; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol Total RNA samples (1000ng) were used as provided by the user. Sample RNA Integrity numbers were 9.2 on average (range 8.9-9.6) when assayed on an Agilent 2100 Bioanalyzer. Sequencing libraries were prepared with the "NEBNext Ultra II Directional RNA Library Prep Kit for Illumina" (NEB #E7760) as recommended by the kit manufacturer. Briefly, polyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters. Adapter-ligated library was completed by PCR with Illumina PE primers. {This kit generates directional libraries stranded in the antisense orientation [the read1 (the only read in single read format) has the antisense orientation (--library-type fr-firststrand in TopHat, --stranded=reverse in HTSeq]} The resulting purified cDNA libraries were applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument by following manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4555803