title: RNAseq of Vitis vinifera: flower EL18-19 clone VP25_3
design: The library was constructed from flowers of a replicate of Vitis vinifera cv. Tempranillo. flowers collected from one plant were used for each replicate. The library was prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit starting from 0.250 ng-1 ug of total RNA. The adapter-ligated library was amplified with 15 PCR cycles, followed by purification with Ampure beads. Finally, the library was run on a Bioanalyser DNA1000 chip, and quantified using a real-time PCR kit (Kapa Biosciences). Sequencing was performed on the Illumina HiSeq 2500 at high output mode using v4 chemistry (flowcells and sequencing reagents).