title: GSM2826018: L540_RP2_6h; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using RNeasy Mini Kit (Qiagen), and treated with DNase according to the manufacture's protocol. Total RNA quality was evaluated using the Agilent Bioanalyzer and the RNA Nano kit: only samples with RIN score ≥ 8 were processed further. RNA-seq libraries were sequenced using the TruSeq mRNA stranded kit (Illumina) according manufacturer's indications. Briefly, the polyA fraction was isolated starting form 300 ng of high quality total RNA. All libraries were quantified using Qubit (Thermo Fisher Scientific Logo) and Bioanalyzer 2100 (Agilent) instruments. According to index compatibility, the libraries were grouped into pools at a concentration of 8 pM to obtain ~15 million clusters for each sample, for single run. Single end sequence reads (75-bp in length) were generated on an Illumina NextSeq 500 (Samples 1 to 16)
attributes.tag: GEO Accession
attributes.value: GSM2826018