title: GSM1266739: WT-82 y iPSC-mDA + Nuclear GFP n 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed directly in Trizol (Life Technologies).   RNA was extracted using chloroform and ethanol precipitation and further cleaned using the miRNeasy kit (Qiagen). After ribogreen quantification and quality control of Agilent BioAnalyzer, 500ng of total underwent polyA selection and Truseq library preparation according to instruction provided by Illumina (TruSeq™ RNA Sample Prep Kit v2), with 6 cycles of PCR. Samples were barcoded and run on a Hiseq 2000 in a 75bp/75bp Paired end run, using the TruSeq SBS Kit v3 (Illumina).  An average of 74 million paired reads were generated per sample. At the most the ribosomal reads represented 0.3% and the percent of mRNA bases was closed to 55% on average.
attributes.tag: GEO Accession
attributes.value: GSM1266739