title: GSM2476291: WT_pyx1; Mus musculus; RNA-Seq
library_construction_protocol: GFP+ Corticospinal motor neurons from crym-GFP transgenic wild type and ngr1-/- mice were harvested via laser capture microdissection from flash frozen brains, cut on a cryostat. RNA was harvested with QIAGEN Rneasy Micro kit (cat#74004). 500pg of total RNA was processed with Clontech's SMARTer Ultra Low Kit (cat# 634888) was used for the construction of sequencing libraries. Covaris AFA system was used prior to standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM2476291