title: GSM4669959: Gestational diabetes Patient 5, 3rd Trimester (RNA-seq); Homo sapiens;    RNA-Seq
library_construction_protocol: Peripheral venous blood was collected into      EDTA-coated tubes. Blood was then centrifuged at 2000 X g for 7 minutes at 4°C,      in order to collect only the plasma fraction. Plasma was centrifuged at 16000 X      g for 10 min at 4°C to remove all the residual cells, aliquoted and stored at      -80°C. Cell-free RNA was extracted from 200 µL of plasma using the miRNeasy      serum/plasma kit (Qiagen). Total RNA was quantified using the Qubit RNA HS Assay      kit (Thermofisher Scientific). RNA integrity was verified using the Agilent High      sensitivity RNA Screen Tape System (Agilent) on a 2200 TapeStation System      (Agilent,). The RNA-sequencing (RNA-seq) library construction was performed      using the ultra-low input specific kit, NuGen Ovation SoLo RNA-Seq kit with      Human rRNA AnyDeplete kit (NuGen), according to the manufacturer's instructions.      Libraries were sequenced using 100 bp single-end reads on the Hiseq4000 System      (Illumina)