title: GSM3082648: SB6; Homo sapiens; MBD-Seq
library_construction_protocol: DNA extracted extracted using the Invitrogen PureLink Genomic DNA Mini kit. ). Briefly, 50-85 mg of manually dissected glandular tissue was lysed with proteinase K (20 g/L) for 4 h at 55 °C and 300 rpm. The lysate was centrifuged (3 min at 17968 g, room temperature) and treated with RNase A (20 g/L in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA; 2 min at room temperature). The sample was purified through a silica-based membrane and gDNA was eluted twice with 50 µL of water. DNA quantity and fragment length were measured using Qubit dsDNA HS assay (Invitrogen, Merelbeke, Belgium) and 1% agarose gelelectrophoresis, respectively, and all samples were diluted to a final concentration of 500 ng gDNA/75 µL water. MBD2 protein methyl-CpG binding domain
attributes.tag: GEO Accession
attributes.value: GSM3082648