title: miRNA seq of Colossoma macropomum: Juvenile skin
design: The libraries were prepared according to manufacturer’s instructions for the TruSeq SBS kit v3, by Illumina Inc (Illumina, San Diego, CA, USA). The workflow comprised three main steps: ligation of 5’ and 3’ adapters; Amplification phase; selection of fragments with nearly 20 nucleotides in polyacrylamide gel 10% (w/v).