title: GSM3146557: MT_Total_RNAseq; Mus musculus; RNA-Seq
library_construction_protocol: For total RNA-seq, total RNAs were extracted from MB or MT C2C12 cells using TRIzol reagent (Invitogen) and then delivered to BGI HK for rRNA depletion; for poly A+ RNA-seq, RNA-seq was performed following the procedures described before (Chen et al., 2017; Zhou et al., 2015). Total RNAs were extracted from C2C12 cells using TRIzol reagent (Invitogen) following the manufacturer's instructions and then subjected to poly(A) selection (Ambion, 61006). For total RNA-seq, RNAs were submitted to Beijing Genomic Center, Hong Kong. The libraries were prepared following strand specific RNA-seq library preparation procedures and sequenced on the HiSeq2000 platform; for polyA+ RNA-seq, we prepared the libraries using NEBNext® Ultra™ II RNA Library Preparation Kit (NEB, E7770S) and barcoded libraries were pooled at equal concentration and sequenced on the HiSeq1500 platform.
attributes.tag: GEO Accession
attributes.value: GSM3146557