title: GSM4490658: CTX GM, rep2; Mus musculus; RNA-Seq
library_construction_protocol: mRNA transcripts were isolated from the tissue as described before (Sanz et al., 2009). RNA purification was performed using RNeasy Micro Kit (Qiagen 74004). cDNA was prepared from the isolated RNA using Ovation RNA-Seq System v2 (NuGEN 7102).  Amplified cDNA was fragmented and libraries were prepared using IonXpress plus gDNA & Amplificon Library Preparation kit (4471269, 4471252, 4471250). Samples were barcoded during the library preparation with sample specific indices. Equimolar ratio of each sample was then pooled and sequencd on an Ion Proton Sequencer.
attributes.tag: GEO Accession
attributes.value: GSM4490658