title: GSM3003490: FL DC1 rep2; Mus musculus; RNA-Seq
library_construction_protocol: Cells were harvested with Trizol reagent and stored at -80 °C until use. Total RNA was extracted using the PicoPure kit (KIT0204). RNASeq libraries were prepared using the Clontech Ultra low RNA kit, starting with 3 ng, with 10 cycles of PCR for cDNA amplification, and the Clontech Low Input Kit for library prep, with 7 cycles of PCR amplification, following the manufacturer's protocol. The amplified library was purified using AMPure beads, quantified by Qubit and QPCR, and visualized in an Agilent Bioanalyzer. The libraries were pooled equimolarly, and run on a HiSeq 2500, as 50 nucleotide in length.
attributes.tag: GEO Accession
attributes.value: GSM3003490