title: RNA-Seq of kleptoplastidic dinoflagellate (RSD) under different temperature and light conditions
design: Ross Sea Dinoflagellate was enriched away from the prey and grown in f/2 + Si at 0C under 14/10hr light dark cycle at 26.2  mol/m2/sec. The culture was split into 4 replicates with approximately 450,000 cells per flask and incubated at constant illumination 48  mol/m2/sec (deep chlorophyll maximum) at 0C for 5 days. Cultures were fixed with RNALater and harvested by filtration onto 0.8 micron pore size polycarbonate filters. Cells were lysed using the Qiagen RNeasy Mini Kit.  DNA was removed by DNase treatment on the purification column.  mRNA was isolated using ribosomal depletion (Ribo-Zero  rRNA Removal Kit for Plant Leaf).  RNA quality was checked by Bioanalyzer and sent to Sulzberger Columbia Genome Center for library preparation and RNA-Seq.