title: NextSeq 500 sequencing; RNA-seq in HUVECs overexpressing Sox18 and treated with Sm4
design: RNA-seq in HUVECs overexpressing Sox18 and treated with Sm4
library_construction_protocol: HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2.  Transfection (7 hours) was performed using Roche X-tremegene DNA transfection reagent in 0.4% FBS media.  Directly following transfection, cells were stimulated (14 hours) with small molecule (or DMSO), in 0.4% FBS media. RNA was extracted using RNeasy mini kit (Qiagen). Samples (>100 ng RNA) were processed for whole transcriptome sequencing using TruSeq stranded total RNA library prep kit (Ribo‐Zero GOLD, Illumina).
attributes.tag: Experimental Factor: none
attributes.value: compound
attributes.tag: Experimental Factor: transfected with Sox18-cMyc
attributes.value: genotype