title: GSM4058341: CTX_PV_2; Mus musculus; ATAC-seq
library_construction_protocol: We crossed Sst-IRES-Cre (The Jackson Laboratory Stock # 013044), Vip-IRES-Cre (The Jackson Laboratory Stock # 010908) and Pv-Cre (The Jackson Laboratory Stock # 017320) with SUN1-2xsfGFP-6xMYC (The Jackson Laboratory Stock # 021039) and used adult (6-12 wk old) male and female F1 progeny. Nuclear purifications were performed from whole cortex of adult mice as previously described using anti-GFP antibodies (Fisher G10362). Isolated nuclei were gently resuspended in cold L1 buffer (50 mM Hepes pH 7.5, 140 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.25% Triton X-100, 0.5% NP40, 10% Glycerol, protease inhibitors), and pelleted at 800g for 5 minutes at 4°C. DNA libraries were prepared from the nuclei using the Nextera DNA Library Prep Kit (Illumina) according to manufacturer's protocols.  The final libraries were purified using the Qiagen minelute kit and sequenced on a Nextseq 500 benchtop DNA sequencer (Illumina). 2 replicates were generated and sequenced for each mouse strain.
attributes.tag: GEO Accession
attributes.value: GSM4058341