title: GSM3983989: muscle_20wk_3; Nothobranchius furzeri; RNA-Seq
library_construction_protocol: RNA was isolated from skeletal muscle tissue from 3 fishes for each condition (young, adult and old) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and libraries integrity were verified with LabChip Gx Touch 24 (Perkin Elmer). 1µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in average of 30M reads per library with 2x75bp paired end setup.