title: Illumina MiSeq sequencing; miRNA-seq of microRNAs from control or PARN depleted HeLa cells
design: miRNA-seq of microRNAs from control or PARN depleted HeLa cells
library_construction_protocol: HeLa cells were transfected with siRNAs and harvested in RNA lysis buffer (Quick RNA miniprep kit from Zymo Research) after 72 hours. RNA was extracted according to Quick RNA miniprep kit protocol from Zymo Research. 1 microgram of total RNA was used for library prep using NextFlex Small RNA-Seq kit V3 from Bioo Scientific.
attributes.tag: Experimental Factor: RNA interference
attributes.value: scrambled siRNA