title: GSM4420203: KP tumor d30 Ams #3; Mus musculus; ATAC-seq
library_construction_protocol: Sorted TRMs in the lung of naïve and KP-tumor bearing mice at 2 different time points (d15 and d30 post-tumor transplantation). For library preparation: cells were lysed and incubated for 30 min at 37 °C with 50 µl of transposase reaction, which contained 25 µl of Tagment DNA buffer (Illumina), 2.5 µl of Tagment DNA enzyme (Illumina), 0.5 µl of NP-40 and 7 µl of nuclease-free water. The transposed DNA was immediately purified with the Qiagen MinElute PCR purification kit according to the manufacturer's instructions and eluted in a 10-µl volume. PCR amplification of the transposed DNA was done with a low-cycle number protocol and with published primers.
attributes.tag: GEO Accession
attributes.value: GSM4420203