title: RNA-Seq of RCC liver
design: Synthesis of cDNA was performed using a SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA). After PCR optimization, amplified cDNA was fractionated into 1-2 kb, 2-3 kb and >3 kb fractions by BluePippin Size Selection System (Sage Science, Beverly, MA) and were used to construct three libraries of different insert sizes using the SMRTbell Template Prep Kit 1.0. Libraries were sequenced by PacBio Sequel platform.