title: Illumina HiSeq 2000 paired end sequencing; Swine DNA methylation study using RRBS : FAANG
design: DNA methylation analysis
library_construction_protocol: EZ DNA Methylation™ Kit
attributes.tag: assay type
attributes.value: methylation profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 12
attributes.tag: extraction protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/samples/KU_SOP_Preperation_of_Genomic_DNA_from_Small_Samples_20170516.pdf
attributes.tag: library preparation location
attributes.value: Shenzhen, China
attributes.tag: library preparation location longitude
attributes.value: 114.0683000
attributes.tag: library preparation location latitude
attributes.value: 22.5455400
attributes.tag: library preparation date
attributes.value: 2013-08-20
attributes.tag: sequencing location
attributes.value: Shenzhen, China
attributes.tag: sequencing location longitude
attributes.value: 114.0683000
attributes.tag: sequencing location latitude
attributes.value: 22.5455400
attributes.tag: sequencing date
attributes.value: 2013-08-20
attributes.tag: experiment target
attributes.value: DNA methylation
attributes.tag: library selection
attributes.value: RRBS
attributes.tag: bisulfite conversion protocol
attributes.value: http://www.zymoresearch.com/downloads/dl/file/id/197/d5007d.pdf
attributes.tag: pcr product isolation protocol
attributes.value: https://www.epigenesys.eu/images/stories/protocols/pdf/20160127163832_p70.pdf
attributes.tag: bisulfite conversion percent
attributes.value: 99.5
attributes.tag: restriction enzyme
attributes.value: MspI
attributes.tag: min fragment size selection range
attributes.value: 40
attributes.tag: max fragment size selection range
attributes.value: 220