title: NextSeq 550 sequencing; Myc-dependent transcriptome of Drosophila L3 larval wing imaginal discs, and its dependency on Max and/or the PAF1 complex component Atu/Leo1
design: Myc-dependent transcriptome of Drosophila L3 larval wing imaginal discs, and its dependency on Max and/or the PAF1 complex component Atu/Leo1
library_construction_protocol: 48 hours after the heat-shock, wing imaginal discs were dissected into Qiazol & stored at -80C Drosophila melanogaster were grown on standard food at 25C larvae were subjected to a 2-hour 37C heat-shock (48 hours before collection) total RNA was extracted from wing imaginal discs using the Qiazol method cDNAs were prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (Illumina). Sequencing libraries were prepared with the NEBnext mRNA Library Prep Master Mix set for Illumina.
attributes.tag: Experimental Factor: Max knockout
attributes.value: phenotype