title: Exome Sequencing of XP-V complemented human fibroblasts
design: Control cells were maintained in the dark during irradiation under similar conditions. Cells were grown for 10-15 days, and clones were randomly selected and transferred to 96-multiwell plates using cloning discs with trypsin. As soon as the clones reached confluence, they were transferred to a larger dish. Cells from two 75 cm2 flasks were used for genomic DNA extraction . The selection of clones immediately after irradiation led us to detect induced mutations only in the first round of DNA replication for each cell.Exome sequencing was performed after UVA irradiation. Libraries were prepared with 5 g of genomic DNA using the Sure Select QXT kit. Sure Select QXT Human All Exon V6 baits were used for the exome capture procedure. Libraries were sequenced in paired-end mode (2x125 bp) on the Illumina HiSeq platform.