title: GSM3983139: 2895_M32; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs were isolated at the Ifakara Health Institute by density gradient centrifugation, cryopreserved in liquid nitrogen and shipped to the Babraham Institute where the PBMC were thawed and rested for 2 hours at 37 degrees C. Cell number and viability were measured using a Nucleocounter (NC-3000, Chemometec). Samples with low viability (<50% live cells) were discarded. Peripheral blood mononuclear cells from Tanzanian children at B0 and B32 post thaw were stored in RNA-later. RNA from samples with high viability (>90%), sufficient cell number (>3x10^6 cells) were isolated using RNeasy plus Mini kit (Qiagen). Samples with high RNA quality (RIIN >7) were used Libraries were generated from samples with high RNA quality (RIIN >7) using TruSeq Stranded mRNA Library prep (Illumina) by Eurofins.
attributes.tag: GEO Accession
attributes.value: GSM3983139