title: RNA seq of Ganoderma Copper Induced
design: Total RNA (~4 g) was used to prepare the RNA seq library using the TruSeq RNA Sample Prep Kits (Illumina). The libraries were prepared as per the kit protocol. In short, poly-A containing mRNA molecules was purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments were used to synthesize first strand cDNA using reverse transcriptase and random primers followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments were than go through an end repairing process, the addition of a single  A  base, and then ligation of the adapters.