title: RNA-SEQ of canis familiaris: dog with otitis externa
design: The Ribo-Zero rRNA removal kit (Epicentre, USA) was used for ribosomal RNA depletion according to manufacturer instructions. Libraries for Illumina sequencing were made with the TruSeq Stranded mRNA sample prep kit (Illumina, USA) following the manufacturers protocol. RNA sequencing was performed on the Illumina HiSeq 2500 platform using single-end 50bp sequencing.  The sequence data for the reference genome was retrieved from the NCBI database. Quality-filtered reads were aligned to the reference-genome sequence using Bowtie2. The relative transcript abundance was measured in fragments in reads per kilobase of exon sequence per million mapped sequence reads (FPKM).