title: GSM2899168: germline_wt1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Early embryos, TJ>UAS-l(3)mbt::myc;l(3)mbt[GM76]/+ and TJ>UAS-l(3)mbt::myc;l(3)mbtGM76/Df females were allowed to lay for 30 minutes to 1 hour on agar plates. Embryos were dechorionated in 50% bleach for 5 minutes, rinsed with PBS, and then lysed in TRIzol following manufacturer's guidelines. Libraries were generated from 1µg of total RNA using the NEBNext Poly(A) magnetic Isolation Module (NEB #7490) and the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB # E7420). Libraries from 2 biological replicates were sequenced on an Illumina Hi-Seq2500, paired-end 50 run.
attributes.tag: GEO Accession
attributes.value: GSM2899168