title: NextSeq 500 sequencing; microRNA-seq of miRNAs isolated from control or DIS3L2 knockdown HeLa cells
design: microRNA-seq of miRNAs isolated from control or DIS3L2 knockdown HeLa cells
library_construction_protocol: HeLa cells were transfected with control or DIS3L2 siRNA and harvested after 72 hours using RNA lysis buffer (Quick RNA miniprep kit from Zymo Research). RNA was extracted according to manufacturer's protocol (Quick RNA miniprep protocol). 1 microgram of total RNA was used for library preparation using NextFlex Small RNA-seq kit v3 from Bioo scientific.
attributes.tag: Experimental Factor: RNA interference
attributes.value: DIS3L2 knockdown by siRNA