title: Illumina MiSeq paired end sequencing
design: V3-V4 16S rRNA using primers 341F and 806R
library_construction_protocol: DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V3-V4 hypervariable region of bacterial and archaeal 16S rRNA genes, and to attach MiSeq adapter and barcode sequences.