title: RNA-Seq of uninoculated Linum usitatissimum leaves - replicate 3
design: Total RNA was isolated from up to 200 mg uninoculated Linum usitatissium leaves using the RNeasy midi kit (Qiagen), according to the manufacturers instructions.  Libraries were made and 50 bp single reads were generated in one lane according to standard Illumina HiSeq protocols.  The sequence reads were analysed according to Australian Genomic Research Facility quality control measures.  Sequence quality was >95% bases above Q30.  The reads were also screened for the presence of any Illumina adaptor/overrepresented sequences and cross-species contamination.  The primary sequence data was generated using the Illumina consensus assessment of sequence and variation (CASAVA) pipeline (version 1.8.2).