title: RNA-seq of Arapaima gigas: juvenile female skin
design: The construction of libraries was accomplished in two major steps, comprising the fragmentation of total RNA and synthesis of cDNA double-strands, which comprises the following steps: denaturation of RNA with addition of randomic primer, synthesis of the first and second strands of cDNA and purification. The following steps were enrichment and preparation of the plate for sequencing in the platform GS FLX sequencer 454.