title: Porites lobata healthy 5
design: Sequences were trimmed (between the 10th and 190th base-pairs) and selected based on quality scores for downstream taxonomic and statistical analyses in QIIME 2 (https://qiime2.org). The dada 2 quality control algorithm (Callahan et al. 2016) was applied to the sequencing data to retrieve the frequencies of amplicon sequence variants (ASVs) and prevent an inflation of the observed number of taxa (Edgar 2017). A phylogenetic tree was generated using the FastTree software (Price et al. 2010). The taxonomic identity of ASVs was determined using the Greengenes RNA database (Caporaso et al. 2010) and a multinomial naive bayes classifier trained for the selected V4 sequence (see above) in QIIME 2. Any sequences which were not successfully classified by the Greengenes classifier were manually blasted to ensure that no match was truly present. If a match was found, this was substituted into the ASV table for further downstream analysis.