title: DNA-Seq of C. nemoralis: foot
design: gDNA was converted into a SMRTbell library using Procedure & Checklist - Preparing >30 kb Libraries Using SMRTbell Express Template Preparation Kit. The insert size of the final library was then determined on Fragment Analyzer (Agilent Technologies). To increase the sequencing read length, an additional damage repair was performed on the library. The library was annealed with sequencing primer V4 and binding was done using binding kit version 3. The library was sequenced with 20 hr movie-time using Sequel Sequencing kit v3.0 chemistry on 12 PacBio Sequel SMRT cells, generating 7,202,997 subreads.