title: GSM3684401: WH-MeDIP-Seq 2; Sus scrofa; MeDIP-Seq
library_construction_protocol: DNA was isolated from tissue with Qiagen DNeasy tissue kit (Qiagen, velencia, CA). Purified DNA was analyzed on a ND-8000 spectrophotometer (Nanodrop, Technologies, Wilmington, DE), and Qubit (invitrogen,CA,USA), to determine the quantity. High quality DNA (3 μg) was sonicated at intensity 4 for 200 cycles per burst for 55 s  (Covaris S2), and DNA fragments were end repaired, ATP tailed, and adapter ligated with the Sample Preparation Kit(Illumina). Then DNA was recovered by AMPure XP Beads Libraries were using the Magnetic Methylated DNA Immunoprecipitation Kit (Diagenode) according to the manufacture's protocol.
attributes.tag: GEO Accession
attributes.value: GSM3684401