title: Tumor liver RNA-Seq
design: RNA quality was confirmed by gel electrophoresis. RNA preparation for 1428N and 1428T were carried out according to the manual of the RNeasy Mini Kit (Qiagen), with an extra on-column DNase digestion for sample preparation. Free of DNA contamination and RNA integrity was first checked by gel electrophoresis. RNA samples for RNA-Seq experiment were further checked with Agilent Bioanalyzer to assess sample integrity. RNA preparation was carried out according to the manual of the RNeasy Mini Kit (Qiagen), with an extra on-column DNase digestion for sample preparation. RNA-Seq experiment was performed in the genomic center of National Yang-Ming University.