title: CHH-affected Astacus leptodactylus hepatopancreatic transcriptome expression
design: RNA was extracted from the hepatopancreas of female Astacus leptodactylus at a variety experimental treatments. The treatments include injection to the circulation of L- and D-CHH and sacrifice of the animal one hour after injection as well as RNAs from native and sham injected females. Trireagent extraction kit of Sigma, USA was used for the extraction. The extracted RNAs were further purified using Qiagen RNeasy kit. The various extractions were equally mixed and a total of 10 mg was sent to sequencing after quality examination using bioanalyser (Agilent 2100).