title: GSM4555134: 20FS105A_P4; Mus musculus; Bisulfite-Seq
library_construction_protocol: Frozen tissue samples were homogenized using mortar and pestle and DNA was isolated from ~10mg of samples using the DNeasy Blood and Tissue kit (Qiagen) Reduced representation bisulfite sequencing (RRBS) libraries were prepared according to previously published protocols using gel-free technique (Boyle, P. Genome Biol., 2012).  Briefly, ~500ng of  DNA was digested with MspI restriction enzyme, followed by size selection, gap filling and 3'-A-tailing.  Ligation of NEBNext methylated adaptors was performed, followed by bisulfite conversion and index PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM4555134