title: GSM3901195: Sample_R18059823-cko-2-cko-2; Mus musculus; RNA-Seq
library_construction_protocol: Testes samples were collected from 18-day-old control and Sympk cKOStra8-Cre mice.Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Paired-end libraries were synthesized by using the TruSeq™ RNA Sample Preparation Kit (Illumina, USA) following TruSeq™ RNA Sample Preparation Guide. The cDNA fragments were prepared with cleaved mRNA fragments using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA polymerase and RNase H. These cDNA fragments then go through an end repair process, the addition of a single'A' base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
attributes.tag: GEO Accession
attributes.value: GSM3901195