title: GSM4121243: HONE-1_siNC_1_2_1_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: TRIzol reagent was used to extract total RNA from HONE-1 cell line. We constructed Illumina sequencing libraries according to the modified strand-specific RNA sequencing protocol. Briefly, we isolated non-coding RNA and coding RNA from 3 μg of purified total RNA using a ribo-zero kit for rRNA removal. After isolation, we eluted and fragmented the RNA samples at 94°C for 8 min with Elute-Prime-Fragment Mix. Subsequently, we performed first-strand cDNA synthesis using SuperScriptII (Invitrogen, Carlsbad, CA. USA). The product with Second Strand Marking Master Mix were incubated at 16°C for 1 hour to synthesize the second-strand cDNA. After 3' ends adenylation, we ligated the DNA fragments with TruSeq adapters and the product was amplified using TruSeq PCR primers. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4121243