title: GSM2398375: WT rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using a combination of TRIzol® Reagent (Thermof Fischer Inc.) and RNeasy mini kit (QIAGEN) accrording to the manufacture’s protocol. cDNA libraries were prepared from total RNA using Dynabeads mRNA Direct kit (Thermo Fischer Inc.) an Ion Xpress Plus Flagment Library Kit (Thermo Fischer Inc.). RNAseq libraries were assessed for concentration and length using Agilent High Sensitivity DNA kit (Agilent Biotechnology). Samples were sequenced with using Ion Proton semiconductors sequencers (Theremo Fischer Inc.) according to the manufacturer’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2398375