title: GSM4555211: c12 WT #+2 PBS exp4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA sequencing was carried out on samples collected of C12-cl28 and C12-cl32 under normal growing conditions (PBS) and conditions of RS (150µM HU), for six consecutive passages (passage 1-6) during two independent cultures (n=12 biological replicates per condition). Total RNA was isolated using the miRNeasy micro kit (217084, Qiagen) according to manufacturer's instructions, including on-column DNase treatment. Next, libraries for mRNA sequencing were prepared using the QuantSeq 3' mRNA FWD kit (Lexogen) prior to sequencing on the Nextseq500 (High output, SR75, Illumina). Lexogen quantseq 3' mRNA-seq
attributes.tag: GEO Accession
attributes.value: GSM4555211