title: GSM4491257: HF-2927_ChIP_H3K27ac.umi; Homo sapiens; ChIP-Seq
library_construction_protocol: 2 million cells were crosslinked and lysed in the same way as ChIA-PET. After lysis, the nuclei pellets were sonicated and immunoprecipitated with anti-RNApolII antibody (920102, Biolegend) or anti-H3K27ac antibody (39133, Active Motif). 4 ng of ChIPped DNA or input DNA were subjected to end-repair, A-tailing and adaptor ligation (IDT xGen Dual Index UMI Adapters) with the KAPA Hyper Prep Kit (KK8505, KAPA Biosystems). Adaptor-ligated DNA fragments were PCR amplified with the KAPA Library Amplification ReadyMix (KK2612, Kapa Biosystems) and sequenced on Illumina platform.
attributes.tag: GEO Accession
attributes.value: GSM4491257