title: Single cell RNA-Seq of Mus musculus: control E14 cell line
design: Cells were resuspended in PBS-BSA and processed with a microfluidic device according to the DropSeq Laboratory Protocol V3.1 from McCarrollÕs Lab website [http://mccarrolllab.com/dropseq/] . Per each condition, 3 distinct aliquots of collected emulsion each containing 4000 beads underwent RT and fragment library preparation using Illumina Nextera  XT library preparation kit. A distinct barcode was used for each library to allow subsequent demultiplexing of sequencing reads. mRNA sequencing was performed using an Illumina HiSeq2500 instrument, library fragments were sequenced at 50 bp in PE mode.