title: GSM3984809: Hu_53_Mock; Homo sapiens; RNA-Seq
library_construction_protocol: Tissue blocks were transferred to Lysing Matrix A tubes (MP Biomedicals, Germany) in 600 µl RLT buffer supplemented with 1% β-Mercapthoethanol (Sigma, Germany). Tissue was lysed using FastPrep®-24 (MP Biomedicals, Germany) applying six rounds of tissue lysis at default settings (4 m/s, 20 s). Homogenates were centrifuged for 5 min full speed at 4°C and RNA was purified from the supernatant. Total RNA was reverse transcribed with oligo (dT) primers and RevertAid H Minus Reverse Transcriptase (Thermo Fisher Scientific, USA). The RNA sequencing library was generated from 500ng total RNA using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher) for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacture´s protocols.
attributes.tag: GEO Accession
attributes.value: GSM3984809