title: Genome seqencing  for 10kb mate-pair library of Pangasianodon hypophthalmus: adult male testis
design: DNA from testis tissue of striped catfish  ( Pangasianodon hypophthalmus) was extracted by using a phenol-chloroform extraction protocol. Mate-pair library from 10kb fragments was prepared using The Nextera Mate Pair Library Preparation Kit (Illumina) following the manufacturer s procedure. The mate-pair library was sequenced using Illumina Hiseq2500 sequencing platform.