title: GSM2476258: MCF7 H3K4me2 G0/G1; Homo sapiens; ChIP-Seq
library_construction_protocol: GRO-seq: Nuclear run-on RNA was further purified with TURBO DNA-freeTM kit (Life Technologies,  AM1907) to remove residue DNA contamination. RNA-seq: Total RNA was extracted from synchronized MCF-7 cells with TRIZOL REAGENT (Life Technologies, 15596026) and fragmentized with sonication. ChIP-seq: Chromatin from synchronized MCF-7 cells was digested with Nuclease micrococcal from Staphylococcus aureus (Sigma Aldrich, N3755-50UN) and dialyzed with Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific, PI66380). Dynabeads Protein A (Life Technologies, 10002D) and G (Life Technologies, 10004D) were used for immunoprecipitation with antibodies against H3K4me2 (EMD Millipore, 07-030) and H3K27ac (Abcam, ab4729). GRO-seq and RNA-seq libraries were constructed with Encore Complete RNA-Seq DR Multiplex System 1-8 (Nugen Technologies lnc, 0333-32). ChIP-seq libraries were constructed with ThruPLEX DNA-seq kit (Rubicon) and sequenced to 50bp with Illumina HiSeq machine.
attributes.tag: GEO Accession
attributes.value: GSM2476258