title: GSM4060309: FrB_sKO_F1_A12; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Micro Kit from Qiagen (Cat. #74004) mRNAseq libraries from polyadenylated transcripts were prepared by generating cDNA from 2 ng RNA and 9 PCR cycles per replicate with the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (cat# 634891, Takara) and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit  (cat# FC-131-1096, Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4060309