title: Illumina HiSeq 2000 paired end sequencing
design: 1 microgram of DNA was fragmented with sonification technology (Bioruptor, diagenode, Liege, Belgium). The fragments were endrepaired and adaptor-ligated, including incorporation of sample index barcodes. The products were then purified and amplified (10 PCR cycles) to create the final libraries. The TruSeq DNA LT Sample Prep Kit v2 was used for all steps. After validation (Agilent 2200 TapeStation) all libraries were quantified using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System.
library_construction_protocol: 1 microgram of DNA was fragmented with sonification technology (Bioruptor, diagenode, Liege, Belgium). The fragments were endrepaired and adaptor-ligated, including incorporation of sample index barcodes. The products were then purified and amplified (10 PCR cycles) to create the final libraries. The TruSeq DNA LT Sample Prep Kit v2 was used for all steps. After validation (Agilent 2200 TapeStation) all libraries were quantified using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System.