title: NextSeq 550 sequencing; Transcriptome of S2 cells after depletion of the PAF1 complex component Atu/Leo1
design: Transcriptome of S2 cells after depletion of the PAF1 complex component Atu/Leo1
library_construction_protocol: S2 cells were incubated with dsRNA against GFP or Atu. Total RNA was collected 48h later. Total RNA was isolated using Qiazol, treated with DNase on-column, and then depleted of rRNA using Ribominus. Sequencing libraries were prepared with the NEBnext mRNA Library Prep Master Mix set for Illumina.
attributes.tag: Experimental Factor: GFP dsRNA
attributes.value: RNA interference