title: GSM1930802: LY1_CON_NCINP_R2; Homo sapiens; RIP-Seq
library_construction_protocol: Inputs for RIP, placed directly in Trizol Illumina TruSeq Stranded Total RNA sample prep RIP-seq: Nuclear input samples were taken before 1 mg of nuclear lysate was used for eIF4E RIP. Complexes were eluted by boiling with Tris-EDTA containing 1% SDS and 12% beta-mercaptoethanol. RIP RNA and nuclear input RNA was isolated using Trizol (Life Technologies). mRNA-seq: RNA was isolated using Trizol (Life Technologies). Genomic DNA was eliminate with Dnase treatment, and total RNA was taken into the Illumina TruSeq Stranded Total RNA library prekit (RIP-seq samples) or  mRNA was isolated using two rounds of poly-T oligo attached magnetics beads and then taken into the Illumina TruSeq RNA sample prep kit (PDX RNAseq).
attributes.tag: GEO Accession
attributes.value: GSM1930802