title: Nothofagus_dombeyi_litter_bacteria_with_fertilization 
design: The V4 hyper variable region of the 16s rRNA gene was amplified using the F563-R802 primers fused with 454 adapters within the same primer. Each sample correspond to a pool of five independent samples, each amplified by duplicate PCR reactions to reduce sample and PCR biases, respectively. All amplicons were cleaned using Ampure DNA capture beads (Agencourt- Beckman Coulter, Inc.) and pooled in equimolar concentrations before sequencing on a Genome Sequencer FLX (454-Roche Applied Sciences) using Titanium Chemistry according to the manufacturerÕs instructions.