title: GSM4419223: F2079_3K2_LNG1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using the miRNeasy kit from Qiagen according to the manufacturer's protocol. Purified RNA samples were then cleaned and resuspended in nuclease-free water using the RNeasy MinElute Cleanup kit from Qiagen according to the manufacturer's protocol. RNA quantity was measured using the Nanodrop 1000. Quality of RNA samples was quantitatively assessed by computation of RNA integrity numbers (RINs) using the Agilent RNA 6000 Pico kit (Agilent) following the manufacturer's protocol. Barcoded libraries were constructed using the Ion AmpliSeq Transcriptome Mouse Gene expression assay (Ion Torrent/ThermoFisher Scientific) according to the manufacturer's protocol.  10 ng of total RNA was used as input for library generation. Eight libraries were multiplexed together on one Ion Proton chip and sequenced using the Ion Proton platform (Ion Torrent)
attributes.tag: GEO Accession
attributes.value: GSM4419223