title: GSM1937225: Dam-SG-6; Drosophila melanogaster; OTHER
library_construction_protocol: 50 salivary glands were dissected accurately - fat body was removed completely. DNA was isolated using phenol-chloroform method. 1ug of genomic DNA was digested by DpnI endonuclease. Digested DNA was ligated with double-stranded DNA adapters and then digested with DpnII endonuclease. Next, methylated fragments were selectively amplified using adapter-specific primer. Before the preparation of libraries for Illumina DamID-seq, the adapters used in DamID procedure were cut with DpnII enzyme. No further fragmentation was performed.
attributes.tag: GEO Accession
attributes.value: GSM1937225