title: Aeminium ludgeri E14
design: The DNA library for fungal genome sequencing was prepared from 200 nanograms of high-quality genomic DNA with the TruSeq Nano DNA Library Kit (Illumina, USA) and sequenced using paired-end (PE) 2x150 bp on the NextSeq 550 Illumina platform at Genoinseq (Cantanhede, Portugal). All procedures were performed according to standard manufacturers protocols.Sequenced reads were demultiplexed using Bcl2fastq (Illumina, USA) and quality-filtered with Trimmomatic v.0.30 (6) using the following parameters: 1) bases with average quality lower than Q25 in a window of 5 bases were trimmed, 2) reads with less than 50 bases were discarded and 3) sequencing adapters on reads were removed. High quality, adapter-free reads were assembled with dipSPAdes, v.3.11.1 using the following parameters: --careful and --cov-cutoff auto. Assembled scaffolds with size <1000 bp were removed from the assemblies. Assembly metrics were calculated with Quast version 4.6.1.