title: RNA-seq of Arabidopsis thaliana: CRISPR-Cas9 line SW:cbf2 a  post cold acclimation
design: Samples were prepared using the Illumina TruSeq Stranded mRNA Library preparation kit. Libraries were pooled for multiplexed sequencing. Sequencing was carried out in a 1x50bp single end format using Illumina HiSeq 4000 SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v2.7.6 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.18.0.