title: Crotalus mitchellii pyrrhus WGS
design: Genomic DNA was extracted from snap-frozen liver tissue, stored at 28 degrees Celsius, using standard lysis and proteinase digestion followed by phenol–chloroform–isoamyl alcohol methods. A genomic shotgun sequencing library was prepared by shearing  genomic DNA using a Covaris ultrasonicator, and further preparation using an Illumina Truseq DNaseq library kit. This shotgun library was sequenced on a single lane of an Illumina HiSeq 2000 using 100 bp paired-end reads. Sequencing produced 408 million total paired reads, which were quality trimmed using the Trim Sequences tool in CLC Genomics Workbench (CLC  Bio/Qiagen) with a quality score limit of 0.05 and a maximum of two ambiguities. Trimmed reads were assembled de novo using the De Novo Assembly tool in CLC Genomics Workbench with automatic word and bubble sizes, a minimum contig length of 200 bp, paired-read scaffolding, and reads mapped back to contigs for gap-filling (mixmatch cost = 2, insertion cost = 3,  deletion cost = 3, length fraction = 0.05, similarity fraction =  0.8). The total length of the resulting 478,598 contigs was 1.14 Gb, providing approximately 40x coverage of the genome, with an N50 scaffold size of 5.3 kb.