title: hMeDIP-Seq of Eriocheir sinensis: developing testis2
design: Male crabs at immature stage of testes were purchased from an aquaculture farm in Ganyu County, Jiangsu Province, China. Purified testis genomic DNA was sonicated into short fragments with a size range of 100-500 bp by a Bioruptor. The sheared DNA was then subjected to enrich 5-hmC-containing DNA using the 5-hydroxymethylcytidine antibody. The resulting enriched 5-hmC DNA was then subjected to sequencing library construction. The adapter-ligated 5-hmC library was amplified by PCR.