title: GSM4490936: LCMV; Mus musculus; RNA-Seq
library_construction_protocol: Immediately post-sorting, the indicated B cell populations were run on the 10X Chromium (10X Genomics) and then through library preparation by the Institute for Human Genetics at UCSF following the recommended protocol for the Chromium Single Cell 3' Reagent Kit (v3 Chemistry). Libraries were run on the HiSeq4000 for Illumina sequencing. Post-processing and quality control were performed by the Genomics Core Facility at the Institute for Human Genetics at UCSF using the 10X Cell Ranger package (v3.0.2, 10X Genomics). Reads were aligned to mm10 reference assembly and analyzed using the Seurat and Monocle software packages. Cells were filtered based on number of genes expressed, number of unique molecular identifiers, and the percentage of mitochondrial genes present to exclude outlier cells as described in the Seurat package. Library preparation was performed by the Institute for Human Genetics at UCSF following the recommended protocol for the Chromium Single Cell 3' Reagent Kit (v3 Chemistry).
attributes.tag: GEO Accession
attributes.value: GSM4490936