title: GSM1937111: atphb3.fq; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Root tips were cut, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent (Invitrogen, Carlsbad, CA, USA). Total RNA was extracted from the shoots and roots with TRIZOL reagent (Invitrogen, Carlsbad, CA, USA). First strand cDNA was generated using reverse transcriptase and random primers. The constructed cDNA libraries was qualified and quantified with Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System, and then sequenced via Illumina HiSeqTM 2000 or other sequencer when necessary at the Beijing Genomics Institute, Shenzhen, China.
attributes.tag: GEO Accession
attributes.value: GSM1937111