title: GSM3684262: WGBS-WT T cells; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted with phenol/chloroform method. Genomic DNA degradation and contamination was monitored on agarose. DNA purity was checked using the NanoPhotometer® spectrophotometer. DNA concentration was measured using Qubit® DNA Assay Kit in  Qubit® 2.0 Flurometer  gels. A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer's instructions. Then these DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research), before the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X).  Library concentration was quantified by Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system.
attributes.tag: GEO Accession
attributes.value: GSM3684262