title: NextSeq 500 sequencing; Mouse brown adipocyte single nucleus RNAseq with Smartseq2
design: Mouse brown adipocyte single nucleus RNAseq with Smartseq2
library_construction_protocol: Adult (7-8 week), a mix of 4 male and 4 female mice. Immediately after euthanizing, nuclei from pooled brown adipose tissue from adult (7-8 week), a mix of 4 male and 4 female mice, were isolated with EZ buffer (sigma), stained with DAPI and FACS sorted with RFP+ (AdipoqCre-nlsTdTomato) and DAPI+ channels, into 384-well plate. The mice were kept in room temperature (23 C) throughout their life. They were sacrificed at week 7-8. They had free access to water and common food. Stayed in SPF condition, cages were isolated from the environment and connected with individual air supply, with reversed light cycle (19:00 - 7:00 light on). No clinical symptom was found, checked twice a week by professional animal care takers. FACS direct into lysis buffer. Smartseq2
attributes.tag: Experimental Factor: single cell identifier
attributes.value: Brown adipocyte_181