title: GSM4120182: HCMV-infected HELF cells; Homo sapiens; RNA-Seq
library_construction_protocol: HCMV infected HELF cells were collected at 72 hours post-infection (hpi). Mock-infected cells were harvested as control. Total RNAs were extracted using TRIzol Reagent (ThermoFisher) according to the manufactures' instruction. Then, both of the two group RNAs were subjected rRNA depletion using using Ribo-Zero rRNA Removal Kits (Epicentre) and linear RNA removal using RNase R treatment  (Epicentre) according to the manufactures' instruction. The rRNA-depleted and RNase R treated RNA were fragmented randomly and RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4120182