title: GSM4058745: MEFs-3; Mus musculus; RNA-Seq
library_construction_protocol: RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis Total RNA of each sample was used to prepare the RNA sequencing library 1)RNA fragment；2)Random primed first strand cDNA synthesis；3)dUTP based second strand cDNA synthesis;4)Adaptor ligation and PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM4058745