title: Whole exome sequencing of Felis catus
design: Genomic DNA (250ng) was fragmented targeting 250bp inserts. Libraries were PCR amplified for 8 cycles. The final libraries were purified and quantitated and were pooled pre-capture generating a total 5g library pool.  Each library pool was hybridized with a custom probe set, targeting 35.9Mb of base space. The concentration of each captured library pool was accurately determined through qPCR. The llumina NovaSeq6000 instrument was used to generate 150 bp length sequences yielding an average of 14Gb of data per 35Mb target.