title: GSM3901223: 6h WT R2: 6 hour wildtype C57BL/6J Replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNeasy kit (Qiagen) according to the manufacturer's instructions from 3 individual mice per replicate per time point. The total RNA was then enriched for polyA+ RNA using Dynabeads Oligo(dT)25 (Invitrogen).  The polyA+ RNA was then used to generate RNA-seq libraries. The RNA-seq libraries were subjected to QC analyses (i.e., number of PCR cycles required to amplify each library, the final library yield, and the size distribution of final library DNA fragments) and sequenced using an Illumina Nextseq. 75bp single end polyA-RNA-seq