title: Genome sequencing and assembly of Shigella boydii
design: Genomic DNA was isolated from cultures grown in Lysogeny Broth overnight. DNA was extracted in 96-well format from 100 uL of sample using the MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen, Hilden, Germany) automated on a Hamilton Microlab STAR robotic platform.  Bead disruption was conducted on a TissueLyser II (20 Hz for 20 min) instrument in a 96 deep well plate in the presence of 200 uL phenol/chloroform.  Genomic DNA was eluted in 90 ul water after magnetic bead cleanup. The resulting genomic DNA was quantified by Pico Green. The sequencing libraries were generated with the KAPA HyperPrep Kit. (Catalog number KK8504) and sequenced on the Illumina HiSeq4000 using a 150bpX2 paired end kit.