title: RNA-Seq of Monodon monoceros: adult male liver
design: Monodon monoceros tissue specimens were processed at the Smithsonians National Museum of Natural History (NMNH) in Washington, DC. Total RNA was extracted from 20100milligrams of tissue and were homogenized by cryogenic grinding using a CryoMill (Retsch, Germany). After homogenizing, total RNA was extracted using TRIzol Reagent (Life Technologies, Carlsbad, CA, USA)  following manufacturers instructions. Poly(A) mRNA isolation was conducted with the  Dynabeads mRNA Purification Kit for mRNA Purification from Total RNA preps (Life Technologies) and converted to cDNA using the ScriptSeq v2 Library Preparation Kit (Illumina), both following manufacturers instructions. The cDNA was sequenced on an Illumina MiSeq in the NMNHs Laboratory of Analytical Biology.