title: GSM2899076: EED ChIP-seq of Dnmt3a/3b DKO mESC replicate 2; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% formaldehyde for 10 min at 37°C;Chromatin was fragmented by sonication to obtain fragments 100–700bp in length. For each ChIP assay,5 ug of antibodies were added and incubated at 4◦C overnight. Chromatin was reverse-crosslinked, purified and quantified with a Qubit fluorometer using the Quant-iT dsDNA HS assay kit DNA was end-repaired, adenylated, and ligated to TruSeq sequencing adaptors. DNA was purified by AMPure beads or DNA purification kit according to the DNA amount. Library DNA was amplified by Phusion HF (NEB, Cat. M0530L). The amplified DNA was size selected using 2% agarose gel for 200-500bp DNA fragments.
attributes.tag: GEO Accession
attributes.value: GSM2899076