title: MethylC-seq of Brachypodium: BdTR2k_rep2_spring
design: gDNA isolated from Invisorb plate-based plant kits was processed through Zymo EZ DNA methylation gold 96 Mag-Prep bisulfite conversion under recommended conditions. Libraries created using Swift AccelNGS MethylC-seq kit modified for third reaction volumes and no Covaris fragmentation step. Each sample was given one of 96 possible dual-index barcode combinations and pooled (max 96 sampels) in a HiSeq 2000 flowcell (Single-End 100bp).