title: GSM3382545: CD49f+GFP+817; Mus musculus; RNA-Seq
library_construction_protocol: After FACS sorting, each purified epidermal subpopulation was collected in Trizol-LS (ref) for further RNA isolation as described manufacturer´s instructions Variable amounts of total RNA samples, between 4 and 10 ng, were processed with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) by following manufacturer instructions. Briefly, oligo(dT) primes preferentially reverse transcription on poly(A) RNA in the presence of a template switching oligonucleotide, then limited-cycle PCR produces double stranded cDNA. Resulting cDNA was subsequently processed with the "Nextera XT DNA Library Prep Kit" (Illumina), which fragments and inserts Illumina adapters. Adapter-ligated non-directional libraries were completed by 12 cycles of PCR.  The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina HiSeq 2500 following manufacturer's protocols.
identifiers.namespace: GEO
identifiers.id: GSM3382545