title: Pyrosequencing of nitrous oxide reductase gene nosZII from marine sediments after oxic incubation
design: DNA was extracted from sediments using the Fast DNA Spin Kit for Soil (MP Biomedicals, Santa Ana, USA). A two-step PCR was performed to amplify the nitrous oxide reductase gene nosZII (primers: nosZII-F/nosZII-R, Jones et al., 2013) and introduce the barcode, sequencing key and adapter. Pyrosequencing was performed by Genoscreen (Lille, France) on a Roche’s 454 FLX Genome Sequencer using Titanium chemistry. Sequence data was initially screened and de-multiplexed using QIIME (version 1.8.0, Caporaso et al., 2010) with the default parameters.
identifiers.namespace: Swedish University of Agricultural Sciences
identifiers.id: sediments oxic II