title: GSM3989927: dKO_1_G6; Mus musculus; RNA-Seq
library_construction_protocol: Single bulge hair follicle stem cells were FACS-purified, collected into 96 well plates containing lysis buffer and stored in -80oC. Single cells were lyzed by incubating at 72oC for 3 min. Reverse transcription reaction and whole transcriptome amplification were performed according to Smart-seq2 protocol. The amplified cDNA libraries were purified by Ampure XP beads (Beckman Coulter), and tagmentation reaction was performed by Illumina Nextera XT DNA Library Prep Kit. The libraries were pooled and sequenced on Illumina Nextseq 500 with high-output 38/37cycles paired-end mode.
identifiers.namespace: GEO
identifiers.id: GSM3989927