title: GSM4121476: Lung-2; Mus musculus; RNA-Seq
library_construction_protocol: single cells were captured for sequencing library prepration using a 10X Chromium We used the cellranger v2 workflow and processed according to the manufacturer's instructions for a targeted recovery of 1000 cells (10x Genomics, USA). Resulting cDNA libraries were sequenced on an Illumina NextSeq 500 instrument with 1.95pM input and 1% PhiX control library spike-in (Illumina, USA). Single cell capture was conducted at the Boston University Single Cell Sequencing Core using a 10X Genomics Chromium System. The resulting reads were aligned and gene level unique molecular identifier (UMI) counts obtain using Cell Ranger version 2.0.1 (10x Genomics). Four libraries derived from embryonic mouse (two “lung” domain, one “thyroid” and one “forebrain” domain totaling 1316 cells and a post-normalization mean coverage of 165238 reads per cell) were combined for downstream analysis. 3' RNA-seq single cells
identifiers.namespace: GEO
identifiers.id: GSM4121476