title: GSM4622583: Sample 9_Endothelial cells from Eltd1-/- mice brain; Mus musculus; OTHER
library_construction_protocol: GL261 tumors were dissected under a stereological microscope. GFP tagged polysomes was isolated using anti-GFP bound Dynabeads (Qiagen). After extensive washing, mRNA of endothelial cells from healthy brain tissue and GL261 tumor tissue were obtained by Trizol and further purified by RNeasyMicro kit (Qiagen). Ribosome-RNA was removed from the sample using RiboGone kit (Clontech Laboratories). The RNA concentration and quality was measured by Agilent 2100 bioanalyzer. Samples with RIN value >7 were selected for further cDNA synthesis and purification. Three mouse samples from each group (healthy wild type mice, healthy Eltd1-/- mice, GL261 tumor wild type mice and GL261 tumor Eltd1-/- mice) were selected for library preparation using SMARTer® Stranded RNA-Seq Kit (Clontech Laboratories), and were submitted for sequencing on HiSeq2500.
identifiers.namespace: GEO
identifiers.id: GSM4622583