title: GSM2829925: mir845 2n rep2; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: Flowers were harvested in a beaker, covered with 9% sucrose solution and shaken vigorously for 5min to release pollen grains into the solution. The subsequent centrifugation- and filtering-steps were carried out in 9% sucrose solution. Total RNA was extracted using Trizol reagent (Ambion/ Life Technologies, USA) and glass beads (1.25-1.55 mm; Carl Roth). 10 μg of total RNA were run on a 14% TBE UREA Polyacrylamide gel for size selection. Gel slices containing RNA in the range of 17- to 25-nt were purified following the Illumina TrueSeq small RNA protocol for gel extraction. Libraries were constructed using the Illumina TruSeq Small RNA library preparation kit according to the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM2829925