title: ATAC-seq - DonorID: 336 Lamina: GZ: Sample 136
design: "ATAC-seq processing followed the original protocol (Buenrostro et al., Nature Methods, 2013), which involves enzymatic dissociation of tissue using papain inactivated by ovomucoid (Worthington) followed by aliquoting of 50,000 cells, nuclear isolation using an IGEPAL solution, transposition using Nextera Tn5 transposase (Illumina), barcoding and amplification (8-11 cycles), quality control via gel electrophoresis, quantification via qPCR with DNA Standards (Kapa Biosystems), and massively parallel 50 bp paired end (PE) sequencing on an Illumina HiSeq2000 or HiSeq2500 to acquire ~25M fragments per sample. "
identifiers.namespace: UNC-phs001438
identifiers.id: 136