title: GSM1937993: dCas9-BFP-DNMT1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Plus Mini kit (QIAGEN) After DNase I treatment, mRNA were isolated by magnetic beads with Oligo (dT). Mixed with the fragmentation buffer, mRNA were fragmented into short fragments. cDNA was synthesized using the mRNA fragments as templates, resolved with EB buffer for end reparation and ligated with adapters. After size selected and purified by agarose gel electrophoresis, cDNA with sizes about 240 bp were selected for PCR amplification (12 cycles) and library construction.
identifiers.namespace: GEO
identifiers.id: GSM1937993