title: GSM4221312: Eff1-3_B-est_83693; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA extraction of each replicate was performed using the RNeasy Plant kit (QIAGEN). mRNA from each replicate was isolated via Poly(A) RNA Selection kit (Lexogen) using 1 µg of total RNA. Libraries were prepared using the NEBNext® UltraTM II RNA library Prep Kit for Illumina and Index primers from NEBNext Multiplex Oligos for Illumina (New England BioLabs) following manufacturer's instructions. Sequencing was performed on an Illumina Hiseq2500 machine with single-end 50-bp reads
identifiers.namespace: GEO
identifiers.id: GSM4221312