title: GSM4061231: CART_HA_CD4_CM_Day14_HAbulk; Homo sapiens; RNA-Seq
library_construction_protocol: For T cell subset-specific RNA-seq, T cells were isolated from healthy donor buffy coats. Before activation, naïve and central memory CD4+ or CD8+ subsets were isolated using a BD FACSAria cell sorter (Stem Cell FACS Core, Stanford University School of Medicine) using the following markers: Naïve (CD45RA+CD45RO-, CD62L+, CCR7+, CD95-, and CD122-), Central Memory (CD45RA-CD45RO+, CD62L+, CCR7+). Sorted starting populations were activated, transduced, and cultured as described above. On days 7, 10, and 14 of culture, CAR+ CD4+ and CD8+ cells were sorted, and RNA was isolated using Qiagen mRNEasy kit. Samples library prep and sequencing was performed by BGI America (Cambridge, MA)
identifiers.namespace: GEO
identifiers.id: GSM4061231