title: GSM2902727: Astro-WT-GFP-5; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy micro kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser (Pico chips). Full length double strand cDNA librairies were constructed with the Smarter Ultra Low Input RNA kit v4 (Takara-Clontech, Mountain View, CA) with 11 to 14 LD-PCR cycles. Libraries were normalized to 750 pg in 5µl as starting materiel to Nextera XT sample preparation kit (Illumina Incorporated, San Diego, CA). Ligation products were amplified with 12 PCR cycles.
identifiers.namespace: GEO
identifiers.id: GSM2902727