title: GSM3986040: 6636_LV_CAND2_KO_RNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Briefly, snap frozen samples were homogenized in Trizol, mixed with chloroform and centrifuged. The aqueous phase was removed carefully, and RNA was extracted with the RNeasy Micro kit (Qiagen) An aliquot of 200ng for each sample was transferred into library preparation which uses  automated variant of the Illumina TruSeq™ Stranded mRNA Sample Preparation Kit.  This method preserves strand orientation of the RNA transcript. It uses oligo dT beads to select mRNA from the total RNA sample.  It is followed by heat fragmentation and cDNA synthesis from the RNA template.  The resultant 400bp cDNA then goes through dual-indexed library preparation: 'A' base addition, adapter ligation using P7 adapters, and PCR enrichment using P5 adapters.
identifiers.namespace: GEO
identifiers.id: GSM3986040