title: GSM4176241: 25 °C control; Yersinia pseudotuberculosis; RNA-Seq
library_construction_protocol: Total RNA was extracted using a hot phenol extraction protocol as described previously (Nuss et al., 2015, PLoS Genet) rRNA depletion via RiboZero-Kit was followed by sonification to obtain a median fragment size of 200 nt. Afterwards, RNA 5'-ends were phosphorylated using T4 RNA ligase and adapters were ligated to the 5'- and 3'-end. cDNA was produced by reverse transcription, amplified via PCR (15 cycles) and purified via agarose gel (2% (w/v) agarose) electrophoresis (fragments 150-500 bp).
identifiers.namespace: GEO
identifiers.id: GSM4176241