title: GSM4122996: Imiquimod (IMQ) treated; Mus musculus; MeDIP-Seq
library_construction_protocol: Punch biopsies of mouse skin were obtained. Isolation of the epidermis from shaved biopsies was performed by soaking the samples in 0.2% dispase at 4°C for 6 hours and manually separating epidermis from dermis. Genomic DNA from the epithelium of mouse models was purified, sonicated, and ligated to Illumina barcode adapters before hMeDIP-seq. Adaptor-ligated DNA was denatured and incubated with 3 μL of 5-hmC antibody at 4°C overnight. Antibody-DNA complexes were captured by protein A/G beads. The immunoprecipitated DNA was purified and sequenced using the Illumina platform followed by standard Illumina protocols.
identifiers.namespace: GEO
identifiers.id: GSM4122996