title: GSM2060711: elav-GAL4_Pc_n2; Drosophila melanogaster; OTHER
library_construction_protocol: DNA was extracted from the anterior half of torn larvae via Qiagen Blood and Tissue kits as per manufacturers' instructions, digested with DpnI, DamID adaptors ligated, digested with DpnII and amplified via PCR. DNA was sonicated to an average size of 300bp using a Diagenode Bioruptor and DamID adaptors removed via either AlwI or Sau3AI digestion.  Sonnicated material was end-repaired, A-tailed, sequencing adaptors ligated and PCR amplified.
identifiers.namespace: GEO
identifiers.id: GSM2060711