title: GSM2564794: HOXA9/MTV #1.4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using QIAshredder spin columns and an RNeasy Plus Micro kit (Qiagen). RNA was extracted and its quality confirmed using an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). Indexed PolyA libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent, Santa Clara, CA). Libraries were quantified by quantitative PCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Roche). Paired-end 75bp sequencing was carried out by clustering 1.8 pM of the pooled libraries on a NextSeq 500 sequencer (Illumina, San Diego, CA).
identifiers.namespace: GEO
identifiers.id: GSM2564794