title: GSM2482054: Res D9_1; Mus musculus; RNA-Seq
library_construction_protocol: Alveolar macrophages were isolated using bronchoalveolar lavage with phosphate-buffered saline and separated via fluorescence activated cell sorting using previously validated cell surface markers. Isolated total RNA was processed for next-generation sequencing (NGS) library construction as developed in the NJH Genomics Facility for analysis with a Life Technologies (Carlsbad, CA, USA) Ion Proton NGS platform. A modified Kapa Biosystems (Wilmington, MA, USA) KAPA Stranded mRNA-Seq kit for whole transcriptome libraries was used to primarily target all polyA RNA. Briefly, library construction started from isolation of total RNA species, followed by mRNA (poly-A) isolation, 1st and 2nd strand cDNA synthesis, adaptor ligation, amplification, and bead templating. Once validated, the libraries were sequenced as barcoded-pooled samples on a P1 Ion Proton chip, as routinely performed by the NJH Genomics Facility
identifiers.namespace: GEO
identifiers.id: GSM2482054