title: GSM4064145: E3: induced neural stem cells; Homo sapiens; RNA-Seq
library_construction_protocol: Cells of the samples were harvested and filtered with 40 µm strainers to form single-cell suspension The single-cell RNA-seq libraries were generated with the Chromium Single Cell 3` Reagent Kit (10×Genomics), and the single-cell suspension and real-time polymerase chain reaction (RT-PCR) Master Mix were loaded together with Single Cell 3` Gel Beads and Partitioning Oil into a Single Cell 3` chip (10×Genomics) according to the manufacture`s protocol. RNA transcripts from single cells were barcoded and reverse-transcribed within droplets, followed by construction of complementary DNAs (cDNAs) libraries of single cells according to the manufacture`s protocol
identifiers.namespace: GEO
identifiers.id: GSM4064145