title: GSM1616693: AP2-G2 ChIP-seq experiment-1 IP; Plasmodium berghei ANKA; ChIP-Seq
library_construction_protocol: Erythrocytes in the fixed culture were removed by lysis in 0.84% NH4Cl, and the remaining ookinetes were subjected to ChIP. ChIP was performed using the ChIP Assay Kit (Millipore, USA) according to the manufacturer’s protocol. Briefly, samples in the lysis buffer were sonicated with a Bioruptor (Tosho Denki, Yokohama, Japan) until chromatin DNA was fragmented to 150 bp for sequencing with a SOLID 5500 system (Life Technologies). Immunoprecipitation (IP) was performed with anti-GFP antibodies, and the harvested DNA fragments were subjected to sequencing. Input DNAs were obtained from the chromatin without IP. Anti-GFP antibodies used for ChIP were purchased from Abcam (Cat. No. ab290, Lot No.GR19413-1). Single read - SOLiD 5500 (ABI)
identifiers.namespace: GEO
identifiers.id: GSM1616693