title: Methylome of Rhizophora apiculata
design: Young leaves of R. apiculata were collected from Qinlan Harbour, Hainan, China for whole-genome bisulfite sequencing. Genomic DNA was extracted using the modified CTAB protocol (Doyle & Doyle, 1987). Bisulfite treatment of genomic DNA was performed using the Zymo EZ DNA Methylation Lightning Kit (Zymo Research). The bisulfite-treated DNA was purified and used to prepare sequencing libraries using the EpiGnome  Kit (Epicentre). The libraries were sequenced on an Illumina HiSeq 2500 using 100 bp paired-end reads. All procedures followed the manufacturers  instructions.
identifiers.namespace: SUB3879600
identifiers.id: ra_methy