title: 16S rRNA seq of microbiota: feces
design: For ciprofloxacin-treatment experiments, fecal samples were frozen and stored at -80 C prior to DNA extraction. DNA was extracted from whole fecal pellets with the PowerSoil-htp kit (MO BIO, San Diego, CA). DNA extracted from fecal samples was used to generate 16S rRNA amplicons using the Earth Microbiome Project-recommended 515F/806R primer pairs. PCR products were cleaned, quantified, and pooled using the UltraClean 96 PCR Cleanup kit (MO BIO, San Diego, CA) and Quant-It dsDNA High Sensitivity Assay kit (Invitrogen, Carlsbad CA). Samples were pooled and sent to the Mayo Clinic's Molecular Biology Core (Rochester, MN) for 2x300-bp MiSeq (Illumina, San Diego, CA) library prep and sequencing.
identifiers.namespace: SUB6567942
identifiers.id: sample267