title: GSM1940686: D38_AS; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were sorted directly into Trizol LS buffer by Flow cytometry. Total RNA was purified using a miRNAeasy micro kit (Qiagen, USA) and quantified by qPCR as described previously (Seumois et al., 2012) Purified total RNA (5 ng) was amplified following the smart-seq2 protocol (16 cycles of cDNA amplification) (Picelli et al., 2014). cDNA was purified using AMPure XP beads (Beckman Coulter). From this step, 1 ng of cDNA was used to prepare a standard Nextera XT sequencing library (Nextera XT DNA sample preparation kit and index kit, Illumina).
identifiers.namespace: GEO
identifiers.id: GSM1940686