title: GSM2481076: METTL14 Rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitation was performed as previously described (Dawson et al., 2009). Firstly MOLM13 cells were cross-linked with 1% formaldehyde for 10 minutes.  20*10^6 cells were used for each immunoprecipitation with 3ug of specific antibodies or IgG. Immunoprecipitated DNA was purified using the ChIP DNA purification kit (Zymo). Single end 50 bp libraries were prepared using the Bioo Scientific NEXTflex ChIP seq kit following manufacturer's recommendations. Reads were sequenced using HiSeq 1500 and multiplexed reads were split based on their barcodes using Illumina Basespace.
identifiers.namespace: GEO
identifiers.id: GSM2481076