title: GSM3383360: Tcf1-deficient CD4 T cells; Mus musculus; ChIP-Seq
library_construction_protocol: Conventional or regulatory CD4+  T cells were sort-purified from WT Foxp3-cre-GFP mice, and the Tcf1-deficient CD4+ T cells were sorted from CD4-Cre+ Tcf1 fl/fl mice. The cells were then fixed and sonicated to generate chromatin fragments, followed by immunoprecipitation with antiserum against Tcf1, which were then properly washed and immunoprecipitated DNA extracted. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.
identifiers.namespace: GEO
identifiers.id: GSM3383360