title: Illumina HiSeq 2000 sequencing of SAMD00018753
library_construction_protocol: Total RNA and polyA+ RNA were extracted from pools, each of which contained one hundred MII oocytes or 2-cell embryos (C57BL/6 X ICR), by using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Four replicates were made for directional RNA-seq library construction by using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). In this library preparation, cDNAs were enriched by 15-cycle PCR. Illumina HiSeq 2000 was used to perform 50-bp single-end sequencing according to the manufacturer's instructions. 
identifiers.namespace: KYOTO_SC
identifiers.id: DRX019719