title: GSM3538193: ChIP-seq_HEK293-GR_IRF2BP2_dex_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-seq, the HEK293-GR cells were grown on 10-cm dishes in charcoal-stripped medium for 2 days, treated with vehicle or dex (100 nM, 1 h), and fixed with 1% (v/v) formaldehyde 10 min at room temperature. Chromatin was fragmented to ∼200–400 bp using sonication (Bioruptor UCD-300, Diagenode). Anti-IRF2BP2 antibody (A303190A, Bethyl laboratories) was coupled to protein-A- beads (Millipore), fragmented chromatin was incubated with antibody-coupled beads overnight, washed, eluted and de-crosslinked in the presence of proteinase K (Fermentas). Chromatin fragments were purified using MinElute columns (Qiagen). Fragmented de-crosslinked chromatin was used as an input control. Library preparation was done using NEBNext Ultra II kit (New England Biolabs) and sequenced using Illumina Hiseq2000 at EMBL GeneCore (Heidelberg, Germany).
identifiers.namespace: GEO
identifiers.id: GSM3538193