title: unspecified sequencing; qiime_experiment_2382:1263129:188
design: Samples of roots, leaves, soil and rhizosphere from several vineyards in CA
library_construction_protocol: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
identifiers.namespace: CCME-COLORADO
identifiers.id: qiime_experiment_2382:1263129:188
identifiers.namespace: Colorado Center for Microbial Ecology, University of Colorado at Boulder
identifiers.id: qiime_experiment_2382:1263129:188