title: GSM3381231: pEV-U251_Control_Repeat_3; Homo sapiens; RNA-Seq
library_construction_protocol: For whole transcriptome analysis, total RNA was extracted from miR-203 expressing U251 stable cell lines (pmiR-203) and pEV control samples in biological triplicate. RNA extractions were performed using RNeasy Mini Kit (Qiagen Inc., Valencia, CA) with DNase treatment on column following manufacture's instruction. RNA-seq library was prepared using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific.
identifiers.namespace: GEO
identifiers.id: GSM3381231