title: GSM4178181: IU504; Mus musculus; RNA-Seq
library_construction_protocol: After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes.
identifiers.namespace: GEO
identifiers.id: GSM4178181