title: GSM1890777: FPR13H; Pseudomonas aeruginosa; RNA-Seq
library_construction_protocol: 5 mL of the bacteria suspension from each serum bottle was centrifuged at 13,000 x g for 2 minutes, the supernatant was discarded and the pellets were immediately frozen in the liquid nitrogen before storing at -80ºC for later RNA extraction. Total RNA extraction was performed using the QIAGEN miRNeasy Mini Kit (Catalog number: 217004) according to the manufacturer’s instructions except adding an extra bead-beating step to ensure the complete lysis of the bacterial cells. Strand-specific cDNA library construction
identifiers.namespace: GEO
identifiers.id: GSM1890777