title: SD-ES-F-ESC2
design: Total RNA was isolated from cell pellets using TRIzol reagent (Life Technologies) according to the manufacturer's instructions. The RNA integrity was confirmed using a 2100 Bioanalyzer (Agilent Technologies) with a minimum RNA integrity number (RIN) of 8. The mRNA was enriched using oligo(dT) magnetic beads and sheared to create short fragments of approximately 200 bp. cDNA was synthesized using random hexamer primers and purified using PCR product extraction kit (Qiagen). Finally, the sequencing primers linked to the cDNA fragments (approximately 200 bp) were isolated by gel electrophoresis and enriched by PCR amplification to construct the library.
identifiers.namespace: SUB3399476
identifiers.id: SD-ES-F-ESC2 -RNA