title: GSM4672514: Cardiomyocytes_RNA_rep1-TCDD treated_96hrs; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol Reagent (Life Technologies, Carlsbad, CA) according to the manufacturer's instructions. Directional RNA-seq was performed by the Genomics, Epigenomics and Sequencing Core (GESC) at the University of Cincinnati. The RNA quality was determined by Bioanalyzer (Agilent, Santa Clara, CA). To isolate the polyA RNA, NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used with a total of 1 μg of good quality total RNA as input. The NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) was used for library preparation by dUTP-based stranded library. The library was indexed and amplified under a PCR cycle number of 8. After library Bioanalyzer QC analysis and quantification, individually indexed and compatible libraries were proportionally pooled and sequenced using Illumina Hiseq sequencer (Illumina, San Diego, CA). Under the sequencing setting of single read 1x51 bp, about 25 million pass filter reads per sample were generated.
identifiers.namespace: GEO
identifiers.id: GSM4672514