title: GSM3538042: WT 55; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Direct-Zol RNA extraction kit (Zymo Research). Libraries were prepared according to Illumina's instructions accompanying TruSeq Stranded mRNA Library Prep Kit. Briefly, total RNA (0.5µg) from each sample was used to isolate poly(A) mRNA. Steps involved in library preparation were mRNA fragmentation, first strand cDNA synthesis followed by second strand cDNA synthesis and adaptor ligation, cDNA fragments isolation and amplification.
identifiers.namespace: GEO
identifiers.id: GSM3538042