title: GSM3083226: BTPRZ-T.3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA and gDNA was extracted from organoids, tumours and normal control colon tissue using AllPrep DNA/RNA Mini or RNeasy Mini Kit (QIAGEN) or UltraClean® Tissue & Cells DNA Isolation Kit (Mo Bio Laboratories). For RNAseq analysis, 1ug of total RNA was used for sequencing library preparation using a BIOO stranded Poly-A kit (PerkinElmer) according to the manufacturer's instructions and sequenced on Illumina HiSeq to generate 1 x 100bp single-end reads.
identifiers.namespace: GEO
identifiers.id: GSM3083226