title: GSM4278172: FVB/N: PFC animal 3; Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA was extracted from murine brain tissues using the miRNeasy Mini Kit (Qiagen, 217004). Four TgR and three FVB/N mice were used for the prefrontal cortex (PFC), and three TgR and three FVB/N mice were used for the hippocampus. RNA quality and quantity were measured using a Nanodrop 2000 (PeqLab/Thermo Scientific). RNA integrity was tested using the 2100 Bioanalyzer (Agilent Technologies) RNA 6000 Nano LabChip Kits (Agilent Technologies) which revealed RIN values range of 8.6-9.3. 300ng of RNA were subjected to small-RNA sequencing. Libraries were generated using an RNA Library prep kit (NEBNext Multiplex Small RNA Library Prep Set for Illumina, E7300) following manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM4278172