title: GSM1891661: BisSeq_TKO; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA was isolated by phenol chloroform extraction, followed by ethanol precipitation. Library Construction Protocol: 5 μg of sonicated genomic DNA were end repaired and 3′-end adenylated using the Illumina TruSeq DNA LT Sample Preparation kit (Illumina 15025064). Paired-end adapters were ligated to the DNA fragments and adaptor-ligated DNA was purified by 2% agarose gel electrophoresis. The gel-purified DNA was converted with the EpiTect Bisulfite kit (Qiagen). Converted libraries were enriched by 10 cycles of PCR using PfuTurbo Cx Hotstart DNA Polymerase (Agilent) and purified using AMPure XP beads. Quality of the libraries and size distribution was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing was performed on an Illumina HiSeq 2500 machine (100 bp read length, paired end) according to Illumina standards.
identifiers.namespace: GEO
identifiers.id: GSM1891661