title: GSM1399246: Fragilariopsis cylindrus; Fragilariopsis cylindrus CCMP1102; ncRNA-Seq
library_construction_protocol: Cells were washed from the filter using 1 ml lysis buffer from mirVana™ kit (Ambion) and bead beating for 60 sec using a Mini beadbeater (Biospec) to break the cells and release the nucleic acid from the samples. The samples were then centrifuged at 10,000×g for 1 min and the supernatants (750 µl) transferred to a fresh 1.5 ml-microtube. 200 µl additional lysis buffer were added to the filter, bead beating again and centrifuged. The protocol was finished according to the manufacturer´s recommendations. RNA integrity and purity were assessed in 1% (w/v) agarose gel stained with ethidium bromide and on a Nanodrop ND-1000 (Thermo Scientific, DE, USA), respectively Total RNA from T. pseudonana and F. cylindrus was prepared using the small RNA v1.5 kit from Illumina according to manufacturer´s recommendations.  RNA was ligated to the adaptors, reverse transcribed, PCR amplified and purified from a polyacrylamide gel. cDNA libraries were sequenced (42 cycles) using an Illumina Genome Analyzer II at TGAC (Norwich, UK).
identifiers.namespace: GEO
identifiers.id: GSM1399246