title: GSM1618743: WK33-HA mock 14h input rep1; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and WRKY33-DNA complexes were isolated with an HA antibody. ChIP DNA was purified using a QIA quick PCR Purification kit (Qiagen) and subjected to a linear DNA amplification (LinDA) protocol (Shankaranarayanan et al., 2011) which included two rounds of ‘in vitro transcription’ by T7 RNA polymerase. The resulting LinDA DNA was used to generate sequencing libraries bearing barcodes using a NEBNext ChIP-Seq Library Pre Reagent Set for Illumina kit (New England Biolabs).
identifiers.namespace: GEO
identifiers.id: GSM1618743