title: Comparative sequencing of lab-evolved ethanol-tolerant and parental strains of MG1655
design: Samples were prepared following the Illumina Genomic DNA sequencing protocol (Rev. B), using a NEBNext genomic DNA sample preparation kit (New England Biozymes). Genomic DNA was isolated using a DNeasy mini kit (Qiagen). Nebulizers were obtained from Invitrogen. Primers and adapters were obtained from IDT. Adapter sequences included three base pair barcodes to allow multiplexing.
identifiers.namespace: Columbia University
identifiers.id: Ethanol tolerant MG1655