title: GSM4494719: ATAC-seq FSHD iPSC control #1; Homo sapiens; ATAC-seq
library_construction_protocol: cells were processed immediately for ATACseq Cells were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630), and the nuclei were pelleted and resus¬pended in transposase buffer. The Tn5 (Illumina FC-121-1030), and the transposition reaction was carried out for 30 min at 37 °C. After purifica¬tion, the Nextera libraries were amplified for 12 cycles with NEBnext PCR master mix and purified with a Qiagen PCR cleanup kit.
identifiers.namespace: GEO
identifiers.id: GSM4494719