title: GSM3984915: WT-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from OTUD5-wild type, OTUD5-null, and GDC0349-treated HEK293 cells using the RNeasy RNA extraction Mini-Kit (Qiagen) and the quality of the RNA was confirmed by agarose gel electrophoresis. TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. The sequencing library was prepared using the IlluminaTruSeq Stranded mRNA LT Sample Prep Kit (Illumina). The mRNA derived from total RNA using poly-T oligo-attached magnetic beads was fragmented and converted into cDNA. Adapters were ligated to the cDNA and the fragments were amplified by PCR.
identifiers.namespace: GEO
identifiers.id: GSM3984915