title: GSM1697233: total small RNA from krimp mutant ovaries; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: Total RNAs from ovaries were isolated using ISOGEN according to the manufacturer's instructions. Piwi proteins were immunopurified from ovaries using specific antibodies, and associated RNAs were extracted using phenol chloroform extraction. Total RNAs and immunopurified RNAs were separated on denaturing polyacrylamide gels. For total RNAs, radioactively labeled 19mer and 28mer RNA oligos were used as size makers to extract 18-29 nt small RNAs. Small RNA libraries (18-29 nt) were prepared using a TruSeq Small RNA Sample Prep Kit (Illumina) according to the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM1697233