title: GSM3539165: pov_12h rep2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
library_construction_protocol: Cold 5% (v/v) phenol:ethanol was added (1.5 mL per 3 mL sample) to each tube. The cells were pelleted by centrifugation at 4000 RPM at 4°C for 10 min and stored at -80°C for up to two weeks. Total RNA was recovered from pelleted cells with the RNeasy mini kit (Qiagen) and on-column DNase digestion (Qiagen). Ribosomal RNA was removed with capture oligonucleotide mix (MICROBExpress, Ambition). RNA clean-up was performed with NucleoSpin RNA Clean-up (Macherey-Nagel). For the library preparation, the KAPA Stranded RNA-Seq Library Preparation kit for Illumina platforms (Kapa Biosystems) was used according to the manufacturer's instructions. An extra step of size selection was performed using Agencourt AMPure XP magnetic beads (Beckman Coulter).
identifiers.namespace: GEO
identifiers.id: GSM3539165