title: GSM3083465: WCM154 PDOX; Homo sapiens; RNA-Seq
library_construction_protocol: mRNA was extracted from organoids and PDOXs using RNAsy Mini Kit (QUiAGEN) and Maxwell 16 LEV simplyRNA Tissue Kit. Specimens were prepared for RNA sequencing using TruSeq RNA Library Preparation Kit v2 as previously described (Beltran et al., 2016). RNA integrity was verified using the Agilent Bioanalyzer 2100 (Agilent Technologies). cDNA was synthesized from total RNA using Superscript III (Invitrogen). Each sample was then sequenced with the HiSeq 2500 to generate 2 × 75-bp paired-end reads.
identifiers.namespace: GEO
identifiers.id: GSM3083465