title: GSM1402275: Col_2; Arabidopsis thaliana; miRNA-Seq
library_construction_protocol: Total RNA was extracted using TRI-reagent (Molecular Research Center, Inc.) as per manufacturer's instructions. Contaminating DNA was removed by DNase I (Promega, Cat# M610A; Roche, Cat# 04716728001) digestion. cDNA was synthesized from 2 µg total RNAs using reverse transcriptase (Fermentas, Cat# EP0441) and an oligo-dT primer. Quantitative PCR was performed in triplicates on a Bio-Rad IQ cycler apparatus with the iQ SYBR green supermix (Bio-Rad, Cat# 170-8882).Values were obtained by normalizing to UBIQUITIN5. Small RNA libraries were prepared using the Illumina Tru-Seq kit and sequenced with Illumina's HiSeq2000 platform at the UCR Institute for Integrative Genome Biology (IIGB) genomic core facility.
identifiers.namespace: GEO
identifiers.id: GSM1402275