title: GSM4182135: slow#2; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: Total RNA was extracted using the hot-acid phenol method. Library preparation was performed at the RNomics Platform of Université de Sherbrooke. Briefly 10 µg of total RNA was treated with DNase on a RNeasy Mini Kit column (Qiagen) according to manufacturers protocol. RNA integrity was assessed on a 2100 Bioanalyzer machine (Agilent Technologies). Ribo-depleted RNA was isolated from 1.25 µg of total RNA using Illumina RiboZero (yeast). Then, stranded RNA-seq libraries then built using the SSV21106 kit (Epicentre) from the ribo-depleted RNA with 13 cycles of PCR amplification. After assessment of libraries quality and molarity on a DNA HS Chip (Agilent), the libraries were pooled and sequenced in paired-end (2x100 bp) on HiSeq 4000 (Illumina) at McGill University and Génome Québec Innovation Centre (MUGQIC) Sequencing Service at 250x genome depth (35 millions reads) at least  for each sample.
identifiers.namespace: GEO
identifiers.id: GSM4182135