title: GSM2829280: ATAC-seq LPL Treg Ahr KO; Mus musculus; ATAC-seq
library_construction_protocol: [ATAC-seq]  Lamina propria cells were isolated and Tregs were sorted by flow cytometry. [ATAC-seq] Large intestinal CD4+TCRβ+YFP+ Tregs were sorted and subjected to ATAC-Seq according to a published protocol (Buenrostro et al., 2013) with a modification in the library purification step. Briefly, 5x10^4 sorted Tregs were washed once with 50 ul PBS, and then lysed in lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) on ice. Pelleted nuclei were resuspended in transposition reaction mix with Tn5 transposase (Illumina) and incubated at 37℃ for 30 min with gentle shaking. The DNA was purified with MinElute PCR Purification Kit (Qiagen) and was used as a template of PCR with 10 cycles of amplification. The library was cleaned up with 1.2x SPRIselect beads (Beckman Coulter), to exclude the small fragments, before sequencing with Illumia HiSeq 2500.
identifiers.namespace: GEO
identifiers.id: GSM2829280