title: GSM2480349: Dmel_S2_total-RNA-seq_rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For metabolically labeled samples, equal amounts of total RNA (equivalent number of cells) were extracted with Trizol and treated with DNaseI amplification grade (Invitrogen) following manufacturer's guidelines. To enable capture of labeled RNAs these were biotinylated and pulled down with streptavidin beads. 4-thiouridine labeled RNA was eluted with DTT, ethanol precipitated and resuspended in RNAse-free H2O. For total RNA-seq samples, total RNA was extracted with Trizol ethanol precipitated and resuspended in RNAse-free H2O. All libraries were constructed using the TruSeq Stranded Total RNA, with Ribozero Gold  using random hexamers for cDNA synthesis, and ERCC spikes were added to the metabolically labeled samples were based on cell equivalents. nascent RNA-seq, total RNA-seq
identifiers.namespace: GEO
identifiers.id: GSM2480349