title: GSM4278120: 02617-2_S50 (single-end); Vibrio campbellii; RNA-Seq
library_construction_protocol: RNA was extracted using Trizol and chloroform Total RNA was first treated with RiboMinus Transcriptome Isolation Kit (Bacteria) (ThermoFisher Scientific, cat#K155004) to remove excess ribosomal RNA All libraries were prepared using ScriptSeq RNA-Seq Library Preparation kit (Illumina, cat#SSV21124) according to manufacturer's protocol For all samples, sequencing barcodes selected from TruSeq Dual Index Sequencing Primer Box, Paired-End (Illumina cat#PE-121-1003) were added to the libraries during construction for multiplexing. The barcoded libraries were cleaned with AMPure XP beads (Beckman Coulter, cat#A63882), verified using Qubit4 fluorometer (ThermoFisher Scientific) and 2200 TapeStation bioanalyzer (Agilent Technologies), then pooled Pooled libraries were sequenced on NextSeq 500 (Illumina) with NextSeq75 High Output v2 kit (Illumina cat#FC-404-2005) Samples 1-8 were sequenced on single-end 75 cycle run Samples 9-10 were sequenced on paired-end 75 cycle run
identifiers.namespace: GEO
identifiers.id: GSM4278120