title: GSM4221174: Exp2Rep1Day0; Homo sapiens; OTHER
library_construction_protocol: For lineage barcode sequencing, genome DNA (gDNA) was prepared with a QIAmp DNA blood mini Kit (Qiagen). For single-cell transcriptome profiling, accutase-dissociated and filtered GSCs were counted and 10,000 single cells were loaded onto a Seq-Well nanowell array. For low-coverage WGS, gDNA was isolated using DNeasy Blood and Tissue Kit (Qiagen), and then quality and quantity of gDNA was determined by Nanodrop. For lineage barcode sequencing, gDNA was amplified using primers bearing Illumina adaptor and index sequences. Single-cell transcriptome analysis was performed using the Seq-Well method. For low-coverage WGS, 50ng gDNA was used for tagmentation and sample preparation/amplification was performed using the Nextera DNA kit (Illumina) abd Nextera i7/i5 primers (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM4221174