title: GSM4618768: CAG-V5; Mus musculus; ChIP-Seq
library_construction_protocol: For knockout experiments, P0 neocortices were isolated and for over-expression studies, FAC sorting at P3 was performed. The samples were use for RNA isolation using Qiagen minElute RNAeasy Kit Libraries were prepared with Illumina TruSeq mRNA preparation kit (Illumina RS-122-2101) from whole cortices,  NuGEN Ovation V2 system (NuGEN, 0510-32) for Fezf2 over-expressing, FAC sorted cells, and  for ChIP seq experiments using llumina TruSeq ChIP Libary Preparation Kit as per the manufacturer's instructions. Libraries were quality-controlled by Tapestation/ Bioanalyzer analysis and sequenced on Illumina HiSeq 2000 platform at Yale Center for Genome Analysis (YCGA) to generate 75 bp single reads.  NuGEN Ovation V2 system (NuGEN, 0510-32)
identifiers.namespace: GEO
identifiers.id: GSM4618768