title: GSM4492159: IgG control; Mus musculus; MeDIP-Seq
library_construction_protocol: Sample collection protocol: Trophoblast stem cells were grown in culture under standard conditions and washed in PBS prior to DNA and RNA extraction DNA and RNA collected using standard phenol chloroform or trizol extraction protocol respectively ((h)MeDIP-Seq) purified genomic DNA was sonicated to yield 150–600 bp fragments, and adaptors for paired‐end sequencing (Illumina) were ligated using NEB Next DNA Sample Prep Reagent Set 1 (New England Biolabs). Immunoprecipitations were carried out using 500ng DNA per sample, 1.25μg anti-5mC antibody (Eurogentec BI-MECY-0100) or anti-5hmC antibody (Active Motif, 39769), and 10μl Dynabeads coupled with M‐280 sheep anti‐mouse IgG or Protein G (Invitrogen). Pulled-down DNA was amplified for 12 (meDIP) or 15 (hmeDIP) cycles with adapter specific indexed primers. Final clean-up and size selection was carried out with Ampure-XP SPRI beads (Beckman Coulter)
identifiers.namespace: GEO
identifiers.id: GSM4492159