title: Exome-Seq of Mus musculus: adult female tail
design: Sequencing libraries were prepared and captured using SureSelect All Exon V4 Library Kits (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions.  Briefly, 1-200 g of genomic DNA from each sample was fragmented by Adaptive Focused Acoustics (E220 Focused Ultrasonicator, Covaris, Woburn, MA) to produce an average fragment size of ~175 bp.  Fragmented DNA was purified using Agencourt AMPure XP beads (Beckman Coulter).  The quality of the fragmentation and purification was assessed with an Agilent 2200 Tapestation (Agilent Technologies).  The fragment ends were repaired and adaptors were ligated to the fragments.  The resulting DNA library was amplified by 7 cycles of PCR, and 500 ng of each library was captured by solution hybridization to biotinylated RNA library baits for 24 hrs at 65C.  Bound genomic DNA was purified with streptavidin coated magnetic Dynabeads (Invitrogen) and further amplified using 12 cycles of PCR to add barcode tags.  Exome-enrichment libraries were sequenced on the Illumina HiSeq 2500 in Rapid Run mode (Illumina) generating 100100 bp paired-end reads.
identifiers.namespace: SUB5124178
identifiers.id: Mus_002_TAIL_FFT_CTTGTA