title: GSM4064243: E. coli pykF(C8Y) rpoB(H526Y) replicate 1; Escherichia coli BW25113; RNA-Seq
library_construction_protocol: RNA was extracted by pelleting cells for 30 seconds and rapidly resuspending the pellets in 1mL Trizol (Invitrogen, Life Technologies). RNA was extracted from the Trizol suspension using the manufacturer's protocol. The extracted RNA was next treated with Turbo DNase (Ambion, Life Technologies) and further purified using an RNA purification kit (Qiagen). Absence of DNA contamination was confirmed by PCR, where the lack of PCR product (about 100 bp in length) relative to a DNA-containing positive control was interpreted as evidence of DNA removal. RNA-seq libraries were prepared with an Illumina TruSeq stranded RNA kit according to manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM4064243