title: GSM2564440: Combinatorial_screen_A375_T6_R1; synthetic construct; OTHER
library_construction_protocol: Genomic DNA was extracted 2 days after infection (T0) and after 12 doublings (T6) using the QIAamp Blood DNA Midi Kit (Qiagen). For each sample, barcodes identifying sgRNA pairs were extracted from genomic DNA in 24 separate 30-cycle PCR reactions where 2 ug of input DNA was included in each reaction. Illumina adapters where included on the PCR primers for this PCR. Samples were sequenced on the Illumina MiSeq platform.
identifiers.namespace: GEO
identifiers.id: GSM2564440