title: GSM2060828: Late Queen Destined 1; Bombus terrestris; miRNA-Seq
library_construction_protocol: Whole body larvae from the same colony and developmental stage were pooled into eight samples (four for each developmental stage). They were then flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 2 ug of total RNA for the construction of sequencing libraries. miRNA libraries were prepared for sequencing using standard Illumina protocols. However, instead of the Illumina 3' adaptor, a modified 3' HD adaptor with a 4bp degenerate nucleotide sequence (NNNN) on the 5' end was used during the adaptor ligation steps.
identifiers.namespace: GEO
identifiers.id: GSM2060828