title: GSM2566920: Exp 4 Control rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from NuLi cells using the ReliaPrep™ RNA Cell Miniprep System (Promega Corporation) following the manufacturer’s instructions. RNA was eluted in 30 µL of RNAse-free water and stored at -80°C. RNA was quantified using a NanoDrop™ UV Spectrophotometer (Thermo Fisher Scientific Inc) and quality assessed using the Experion™ Automated Electrophoresis System (Bio-Rad Laboratories Pty Ltd). All RNA integrity numbers (RIN) for the analysed samples ranged from 9.7 to 10. mRNA libraries  were prepared at the Ramaciotti Centre for Genomics (UNSW University of New South Wales, Australia). The Illumina TruSeq Stranded mRNA Prep Kit was used for Experiment 4. The six RNA-Seq libraries in Experiment 4 were sequenced  using the Illumina NextSeq 500. R1.fastq and R2.fastq files were produced for each sample.
identifiers.namespace: GEO
identifiers.id: GSM2566920