title: Illumina HiSeq 1500 sequencing; Deregulation of PPAR-beta/delta target genes in tumor-associated macrophages by fatty acid ligands in the ovarian cancer microenvironment - RNAseq
design: Deregulation of PPAR-beta/delta target genes in tumor-associated macrophages by fatty acid ligands in the ovarian cancer microenvironment - RNAseq
library_construction_protocol: Ascites was collected from 30 patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe After Ficoll gradient centrifugation, tumor-associated macrophages (TAMs) were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit.
identifiers.namespace: INSTITUTE FOR MOLECULAR AND TUMOR BIOLOGY PHILIPPS UNIVERSITY  MARBURG GERMANY
identifiers.id: E-MTAB-3167:TA056
identifiers.namespace: Institute for molecular and tumor biology Philipps University  Marburg Germany
identifiers.id: E-MTAB-3167:TA056