title: Azo_dye_Mixture_degrading_enrichment_culture_NH_1C
design: Cell pellet  of the sample was collected by centrifugation  for the genomic  DNA extraction. Universal bacterial primers spanning V1-V3 region of the 16S rRNA gene, i.e., 27 F (5-AGAGTTTGATCCTGGCTCAG-3) and 519 R (5-GWATTACCGCGGCKGCTG-3) were used. After initial amplification, secondary PCR was performed using the above-mentioned conditions. This was done to incorporate specially designed primer sequences with barcodes and linkers. HotStarTaq Plus Master Mix Kit was used. Following PCR, amplicon products from different samples were mixed together and purified using Agencourt Ampure beads. Purified and pooled samples were loaded to the Roche 454 FLX titanium instrument.
identifiers.namespace: SUB6262738
identifiers.id: Enrichment_culture_NH_1C