title: GSM4278287: Sham.H03; Equus caballus; RNA-Seq
library_construction_protocol: Synovial tissue samples were collected arthroscopically from the MCPJ of eleven adult horses before (pre-OA) and 16 weeks after (OA) experimental induction of OA as well as from the sham-operated joints (sham). Synovium samples were placed in RNAlater (Qiagen, Valencia, CA) and stored at -80°C until further processing.  Frozen samples were crushed to powder with a mortar and pestle and placed in tubes containing ceramic beads (Precellys® 2ml Hard Tissue Homogenizing Ceramic Beads CK28, Cayman Chemical, Ann Arbor, MI) and TRIzol reagent (Invitrogen, Carlsbad, CA). Mechanical homogenization was performed for cell lysis (Precellys® Minilys, Bertin Corp., Rockville, MD) prior to RNA extraction on spin columns using the RNeasy Micro Kit (Qiagen, Valencia, CA) per manufacturer instructions. Samples were subjected to standard library preparation and sequencing (100 base-pair, paired-end) on an Illumina HiSeq4000 at the University of Illinois Roy J Carver Biotechnology Center. Libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' and sequenced on an Illumina HiSeq4000. Read 1 aligns to the ANTISENSE strand and Read 2 aligns to the SENSE strand.
identifiers.namespace: GEO
identifiers.id: GSM4278287