title: GSM4332323: Leaves, GmNRPE1-RNAi-T-sRNA-r2; Glycine max; miRNA-Seq
library_construction_protocol: Mature leaf tissues were quickly frozen in liquid nitrogen and ground to a fine powder using a mortar and pestle. Total RNA was isolated from the powder using TRI Reagent from the Molecular Research Center (MRC, TR118) following the manufacturer's protocol. Small RNA library was constructed as previously described (Li, Le et al. 2016; PMID: 27938667). Briefly, 10 µg of total RNA from mature leaf was resolved on a 15% urea-PAGE gel, and the ~15-40 bp RNAs were recovered by gel purification.  Small RNA libraries were constructed following instructions from the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300S). The libraries were pooled and sequenced on an Illumina HiSeq 2500 at the UC Riverside IIGB Genomics Core.
identifiers.namespace: GEO
identifiers.id: GSM4332323