title: GSM2902663: WT_d11hNCC_ATACseq_2; Homo sapiens; ATAC-seq
library_construction_protocol: Approximately 50000 cells were used for each ATAC-seq assay following a previously described protocol (Buenrostro et al. 2013). Briefly, isolated nuclei were resuspended in the transposase reaction mix (25 µl 2x TD buffer, 10 µl transposase (TruSight One Kit), 15 µl nuclease-free H2O) and incubate for 30 min at 37°C. After transposition, DNA was purified using Qiagen MinElute PCR purification kit according to the manufacturer's protocol and eluted in 10 µl elution buffer. Purified DNA samples were used for library preparation and sequencing. ATAC-Seq library was generated by Nextera sample prep kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM2902663