title: GSM3927854: CD4_224_Crit-Ill; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted and purified using Qiagen's RNeasy Mini Kit on the QIAcube. Starting with 350 nanograms of total RNA, barcoded libraries were prepared using Illumina's TruSeq RNA Library Prep totalRNA kit. The quality of the libraries was confirmed using the Agilent Bioanalyzer DNA1000 Assay. The libraries were quantified and normalized by qPCR using KAPA Library Quantification Kit.
identifiers.namespace: GEO
identifiers.id: GSM3927854