title: GSM2828164: KS12; Mus musculus; RNA-Seq
library_construction_protocol: FACS sorted cell pellets were snap frozen prior to lysis and RNA preparation using QIAGEN RNeasy plus mini kit RNA libraries were prepared using Illumina TruSeq Stranded mRNA-seq Sample Preparation Kit (cat# RS-122-2101 and RS-122-2102) following the manufacture's protocol. The RNA libraries were sequenced on a HiSeq 2500. Cluster generation was performed on a cBot with HiSeq PE Cluster Kit v4 - cBot – HS (cat # PE-401-4001) and sequenced with the HiSeq SBS Kit v4 (200 cycles, cat# FC-401-4003). The Hiseq 2500 was operating with HiSeq Control Software v2.2.68 and base-calling was performed with RTA v1.18.66.3. The average insert size (as assessed by electrophoresis) was 216 bp (+/- 4%).
identifiers.namespace: GEO
identifiers.id: GSM2828164