title: GSM3381797: brainless with infection, rep 1; Xenopus laevis; RNA-Seq
library_construction_protocol: Total RNA for each sample was isolated by using TRIzolTM Reagent (Ambion, Invitrogen #15596018) –chloroform, following standard manufacturer's protocol. NGS analyses were performed by The MIT BioMicro Center (Boston, MA). Samples were quality controlled on a Fragment Analyzer (Advanced Analytical) to determine DV200 scores. The mRNA from 1 μg of total RNA was isolated using Illumina human/mouse/rat RiboZero Gold and prepared into Illumina libraries using the Kapa RNA HyperPrep kit and 10 cycles of amplification. Final libraries were pooled and quality controlled using qPCR (Roche LC480II) as well as the Fragment Analyzer and sequenced on HiSeq2000 for 40 nucleotides. Following sequencing, data processing was performed using the standard Illumina pipeline. Fastq files were generated for data processing and assembly.
identifiers.namespace: GEO
identifiers.id: GSM3381797