title: GSM3928743: EXP145-6A; Homo sapiens; OTHER
library_construction_protocol: DNA was extracted using Qiagen DNA mini kit and quantified at Nanodrop. LTR vector-genome junctions were amplified by LM-PCR as previously described (Poletti V, Mol Therapy Meth, 2018). 1 ug genomic DNA was fragmented with Tru91 (Roche) and ligated to a TA-protruding double-stranded DNA linker. After SacI digestion, multiple nested PCRs were performed with specific primers annealing to the linker and vector LTR. Amplicons ranging from 200 to 500 kb were purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). 1 mg of the final libraries was subsequently processed with MiSeq Reagent Kit v3 (2 x 300-bp pair-end sequencing) and sequenced to saturation on Illumina MiSeq System (IGA Technology Services, Udine, Italy).
identifiers.namespace: GEO
identifiers.id: GSM3928743