title: GSM2828294: T47D PADI2 T0 replicate2; Homo sapiens; ChIP-Seq
library_construction_protocol: PADI2 was immunoprecipitated with 6μg of PADI2 antibody (z-22):sc-133877 lot number E1214 and H0715 by 50 μg of chromatin and 42μl of Protein A-Agarose Beads (Diagenode). T47D-MTVL cells, without treatment or transfected with -amanitin resistant HA-tagged wild type (WTr) versus R1810Ar mutant RNAP2 were cross-linked for 10 minutes with 1% formaldehyde. Lysate were sonicated to a DNA fragment size range of 200-300bp using a Biorupter sonicator (Diagenode). PADI2 was immunoprecipitated with 6μg of PADI2 antibody (z-22):sc-133877 lot number E1214 and H0715 by 50 μg and for RNAP2, 150 μg   chromatin was incubated with 20μg of anti-HA (ab9110) antibody followed by 42μl of Protein A-Agarose Beads (Diagenode). Libraries were prepared using standadr Illumina instruction of  NEBNext® ChIP-Seq Library Prep Reagent.
identifiers.namespace: GEO
identifiers.id: GSM2828294