title: GSM4278272: Top2 spike-in ChIP for WT T=3 34C rep2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: For quantitative ChIP-seq samples, 25 ml of the meiotic cells were collected at different time points and fixed for 30 min in 1% formaldehyde. The formaldehyde was quenched with 125 mM glycine. S288c crosslinked meiotic cells were added to respective samples at 20% prior to ChIP processing (Vale Silva et al., 2018). Samples were processed as described in (Blitzblau et al., 2012). Libraries were prepared by PCR amplification with TruSeq adaptors (Illumina) as described in (Sun et al., 2015). Quality of all libraries was checked on 2200 Tapestation. Libraries were quantified using qPCR. The libraries were sequenced on Illumina NextSeq 500 instruments at NYU Biology Genomics core to yield 50 or 100 bp single-end reads.
identifiers.namespace: GEO
identifiers.id: GSM4278272