title: P. multiseries grown at 0.5uM phosphate, 220ppm pCO2, rep C (totalRNA)
design: Total RNA was extracted using Trizol reagent (Thermo Fisher Scientific), followed by genomic DNA removal using TURBO DNA-free Kit (Thermo Fisher Scientific), and Agencourt RNA Clean XP kit (Beckman Coulter) clean up the RNA.In order to construct RNAseq libraries from total RNA, ribosomal RNA was removed from 1.5ug of total using Ribo-Zero Magnetic kits (Illumina). The composition of Removal Solutions constituted a mixture of plant, bacterial, and human/mouse/rat Removal Solution at a ratio of 2:1:1. An Agilent 2100 Bioanalyzer was used to evaluate the quality of rRNA removal. 5ng of rRNA subtracted RNA was used for library construction using a Scriptseq v2 RNA-seq Library Preparation Kit (Illumina). An Agilent 2100 Bioanalyzer was used to verify the mean size of libraries was within a range of 360-370bp.
identifiers.namespace: SUB3878557
identifiers.id: PMLOW220C-PE-IL3-1