title: GSM3610922: Breachers: 3E04-10-P; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Breachers: In collaboration with the Walter Reed Army Institute of Research, to investigate the effects of repeated blast wave exposure in explosive entry personnel (Breachers), serum samples were taken from 12 breachers following training days 1,7,8 and 9. Additionally, genomic DNA was extracted from whole blood collected on training days 1 and 10. Cell-free DNA extraction from plasma or serum was performed using the Analytik Jena PME free-circulating DNA Extraction Kit (845-IR-0003050) using the SE/SBS system only. Bisulfite Conversions were performed using the MethylCode Bisulfite Conversion Kit (Invitrogen, MECOV50). Libaries were constructed using the Nextera transposase (Tn5) enzyme supplied within Illumina Nextera® DNA Sample Preparation Kit (FC-121-1031).
identifiers.namespace: GEO
identifiers.id: GSM3610922