title: GSM4495263: TM5_2_24_2_SynAnt49_4_µg _ml-1; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq
library_construction_protocol: B. subtilis cells were disrupted using a micro-dismembrator. Total RNA isolation was performed with a phenol-chloroform isoamyl-alcohol (25:24:1, pH 5.5) extraction method. rRNA was subtracted from the samples with the Illumina Ribo-Zero rRNA removal Kit (Bacteria) according to manufacturer instructions. The cDNA library was prepared using the NEB Ultra directional RNA library prep kit for Illumina according to instructions and sequencing was performed on an Illumina HiSeq3000 system.
identifiers.namespace: GEO
identifiers.id: GSM4495263