title: GSM3084567: RNAseq_POD5_OP1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from FACS-sorted SSCs, BCSPs, and OPs using the Qiagen RNeasy micro kit. Cells were sorted into 350-700 uL buffer RLT plus and RNA was extracted following kit instructions. 1.5 ng PolyA+ RNA was reverse transcribed following the SmartSeq protocol. 1 ng cDNA/sample was then transposed with Nextera Amplicon Tagment Mix to fragment and add adapters. Libraries were then PCR amplified for 11 cycles and sequenced on an Illumina Nextseq.
identifiers.namespace: GEO
identifiers.id: GSM3084567