title: NextSeq 500 paired end sequencing; RNA-seq of TGF-beta and knockdown of histone variant H2A.Z induced EMT in MDCK cells.
design: RNA-seq of TGF-beta and knockdown of histone variant H2A.Z induced EMT in MDCK cells.
library_construction_protocol: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
identifiers.namespace: Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
identifiers.id: E-MTAB-5628:MDCKD6_3_p