title: WGS of transgenic rice g6h1
design: Total genomic DNA was extracted from rice seeds using the DNeasy Plant Mini Kit (Qiagen). The quality of the extracted rice genomic DNA was evaluated by UV-photospectrometry with a Nanodrop ND-8000 (Thermo Scientific) and 1% agarose gel electrophoresis. The sequencing DNA library was created using Illumina Paired-End library preparation kits and Illumina Oligos for adaptor ligation according to the manufacturer s instructions (Illumina). The mean fragment size was about 500 bp. The constructed DNA library was quantified by Pico-Green (Quant-iT; Invitrogen) and Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). 90 cycles of paired-end sequencing were performed on a single lane of an Illumina HiSeq 2000 DNA sequencer (Illumina). Paired-end sequencing and simple modification of the raw data to ensure sequencing quality were performed by BGI-Shenzhen (Shenzhen, China).
identifiers.namespace: SUB3876021
identifiers.id: 2013R03