title: GSM2829275: RNA-seq LPL Treg Ahr WT (rep 1); Mus musculus; RNA-Seq
library_construction_protocol: [RNA-seq] Lamina propria cells were isolated and sorted by flow cytometry.  Sorted Tregs were lysed in Trizol (Invitrogen), and RNA was subsequently extracted with RNAeasy Mini Kit (Qiagen). [RNA-seq] Total RNA was treated with Ribo-zero kit and RNAseq libraries were generated using kits from Illumina. Barcoded samples were pooled and sequenced over 2 lanes on an Illumina HiSeq 2500 instrument to produce 50 bp single-end reads.
identifiers.namespace: GEO
identifiers.id: GSM2829275