title: GSM3384421: ATAC-seq.DR-/-_rep1; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq libraries of mESCs were prepared as previously described (Buenrostro et al., 2015) with minor modifications. Briefly, samples were lysed in lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2 and 0.15% NP-40) for 10 min on ice to prepare the nuclei. Immediately after lysis, nuclei were spin down, incubated with the Tn5 transposase and tagmentation buffer at 37 °C for 30 min. ATAC-seq library was prepared following a protocol (Buenrostro et al., 2013), and sequenced by Illumina HiSeq2500 at New York University Genome Technology Center following a standard protocol. Paired-end 50bp-length ATAC reads were produced.
identifiers.namespace: GEO
identifiers.id: GSM3384421