title: GSM2402653: LXD_4LE_4; Oryza sativa Japonica Group; miRNA-Seq
library_construction_protocol: Total RNA was extracted from the anthers using Trizol reagent (Invitrogen, CA, USA) following the manufacturer’s procedure. The total RNA quantity and purity were analysis by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 1 μg of total RNA were used to prepare small RNA library according to the protocol of TruSeq small RNA sample preparation Kits (Illumina, San Diego, USA). Then we performed the single-end sequencing (36 bp) on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) according to the manufacturer’s protocol. After the Illumina sequencing, the raw reads were subjected to the Illumina pipeline filter (Solexa 0.3), and then the dataset was further processed with an in-house program, ACGT101-miR (LC Sciences, Houston, Texas, USA) to detected the known and novel miRNA.
identifiers.namespace: GEO
identifiers.id: GSM2402653