title: GSM4493817: RNAseq_young_m6AIP_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol was used to extract total RNA from all samples. Total RNA was fragmented to ~100 bp using chemical fragmentation. The input libraries were generated from approximatley 50 ng of poly(A) + mRNA. For the m6A libraries, total RNA was immunoprecipated using m6A antibody. Libraries were then constructed using the Illumina Truseq RNA Library Preparation kit v2, according to the manufacturer's protcol. An Agilent 2100 BioAnalyzer and DNA1000 kit were used to determine the concentration and quality of the final amplified cDNA libraries.
identifiers.namespace: GEO
identifiers.id: GSM4493817