title: GSM3610931: Triplets: 45W; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Triplets: Dorsolateral prefrontal cortex (DLPFC) and ventral white matter (VWM) were dissected from post-mortem frozen brain tissues from subjects with no diagnosis of any neurodegenerative disease at time of death. Neuronal nuclei were isolated from the DLPFC dissections using methods previously described (Jiang, Matevossian et al. 2008). Briefly, frozen sections of the DLPFC (~100mg) from each subject were homogenized on ice, cells were lysed using a cell lysis buffer, and nuclei were isolated by high-speed centrifugation through a sucrose buffer. Nuclei were immunostained using the Alexa-Fluor conjugated anti-NeuN antibody (Abcam, ab190195) and isolated by FACS (BD FACS-Aria). Cell-free DNA extraction from plasma or serum was performed using the Analytik Jena PME free-circulating DNA Extraction Kit (845-IR-0003050) using the SE/SBS system only. Bisulfite Conversions were performed using the MethylCode Bisulfite Conversion Kit (Invitrogen, MECOV50). Libaries were constructed using the Nextera transposase (Tn5) enzyme supplied within Illumina Nextera® DNA Sample Preparation Kit (FC-121-1031).
identifiers.namespace: GEO
identifiers.id: GSM3610931