title: GSM1888975: WT_RNASeq_E12_AVC; Mus musculus; RNA-Seq
library_construction_protocol: The AVCs were manually dissected from E12.5 hearts, genotyped, and checked for level of Sox9 deletion prior to being pooled for sequencing. RNA was isolated with Trizol using the manufacturer’s protocol. Pooled AVC RNA samples were further purified by GeneJet clean up and concentration micro kit (ThermoFisher Scientific). RNA quality was assessed on the BioAnalyzer (Agilent Technologies). Duplicate RNA-Seq libraries for each genotype were generated and sequenced using Illumina Mi-Seq. RNA-Seq libraries were prepared for sequencing using standard Illumina protocols and performed in duplicate for each genotype.
identifiers.namespace: GEO
identifiers.id: GSM1888975