title: GSM3684857: WUS-GR-MOCK + CHX 2 RNA-seq; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Plant tissues were flash frozen in liquid nitrogen, and Total RNA from apices were isolated using the mirVana™ miRNA Isolation Kit (lifetechnology). 10 μg of totalRNA were subject to DNase I treatment and mRNA was purified by Dynabeads® mRNA DIRECT™ Purification Kit(lifetechnology). Strand-specific RNA-seq libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina following the manufacturer's instructions and were sequenced on the Illumina Hi-Seq2500 platform to obtain 2×100-bp paired end reads.
identifiers.namespace: GEO
identifiers.id: GSM3684857