title: Ascosphaera apis spore
design: Total RNA of Ascosphaera apis spore sample isolated using TRizol reagent (Thermo Fisher, Shanghai, China) on dry ice and thenreversely transcribed using Maxima H Minus Reverse Transcriptase, followed by purification and concentration on a AMPure XP beads. Secondly, cDNA librarieswere constructed from 50 ng total RNA with a 14 cycles PCR using the cDNA-PCR Sequencing Kit (SQK-PCS109) and PCR Barcoding Kit (SQK-PBK004) followingthe manufacturer's protocol (Oxford Nanopore Technologies Ltd, Oxford, UK). Thirdly, the aforementioned cDNA libraries were sequenced by BiomarkerTechnologies (Beijing, China) utilizing PromethION system (Oxford Nanopore Technologies Ltd, Oxford, UK).
identifiers.namespace: SUB7774160
identifiers.id: AaS