title: RNAseq of human keratinocyte in low-oxygen culture
design: RNA from log phase human primary keratinocytes cultures in DermaLife was extracted according to the manufacturers protocol (Qiagen). RNA integrity was verified with the BioAnalyzer (Agilent Technologies) and 3 g of total RNA was sent for sequencing. Ribosomal RNA was depleted before library construction (Truseq mRNA kit, Illumina) and sequenced on the NGS Illumina Hiseq 2000 platform.
identifiers.namespace: SUB5263067
identifiers.id: PatB5