title: GSM1938084: MCF7-LTED_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA (10 ug) was isolated , DNase treated, and twice oligo(dT) selected using the Dynabeads mRNA purification kit (Invitrogen). RNA-seq libraries were constructed using the NEBNext Kit from New England Biolabs. MCF7-LTED_1 libraries were constructed with custom 4 bp barcodes and sequenced with an Illumina GAIIx (32 bp single-end reads). MCF7_LTED_2 libraries were constructed with standard Illumina indexed primers and sequenced using Illumina HiSeq 2000 (42 bp single-end reads).
identifiers.namespace: GEO
identifiers.id: GSM1938084