title: GSM4121528: RNA-seq: T_Cell_WT_Naive_rep4; Mus musculus; RNA-Seq
library_construction_protocol: CD8 T cell subsets were flow cytometrically sorted and resuspended in Trizol. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of Flow cytometrically purified cells were resuspended in Trizol (Ambion Life Technologies) and total RNA was extracted according to the manufacturer's protocol. Quality and quantity of the total RNA was assessed by the 2100 Bioanalyzer using a Nano chip (Agilent). Only RNA samples having an RNA Integrity Number (RIN) > 8 were subjected to library generation. Strand-specific cDNA libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina) according to the manufacturer's protocol. The libraries were analyzed for size and quantity of cDNAs on a 2100 Bioanalyzer using a DNA 7500 chip (Agilent), diluted and pooled in multiplex sequencing pools. The libraries were sequenced as 65 base single reads on a HiSeq2500 (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM4121528