title: IFRC West corner well (399-1-60/C7867) groundwater microbial metagenome Dec 2011
design: Groundwater was prefiltered through a 1.2 um filter and biomass collected on a 0.2 um filter. DNA was prepared using a modified Bostrom protocol (Bostrom et al, 2004, Limnology and Oceanography Methods 2:365) which included freeze-thaw, lysozyme, SDS, proteinase K, and RNase steps. Sequencing was performed at Los Alamos National Laboratory.
identifiers.namespace: SUB948658
identifiers.id: C7867201112