title: MinION sequencing
library_construction_protocol: The gDNA was sheared using g-TUBE spun at 2,000 x g for 2 min (Covaris, Woburn, Massachusetts).  DNA was pretreated with three NEB reagents in this order: PreCR Repair Mix, NEBNext End Repair Module, NEBNext dA-Tailing Module.  We then used the MinION MAP Standard Protocol for the gDNA Library Preparation Kit v004 (Oxford Nanopore Technologies, Oxford, UK) with no changes in the recommended steps.  The same library was run on two different flow cells.
identifiers.namespace: Virginia Commonwealth University
identifiers.id: ena-EXPERIMENT-Virginia Commonwealth University-05-03-2015-14:24:31:055-2