title: GSM4618984: ID12_VisitB_MeDIP-seq; Homo sapiens; MeDIP-Seq
library_construction_protocol: Plasma was separated from whole blood and cfDNA was extracted using the Qiagen circulating nucleic acids kit. Libraries were prepared using a published protocol (PMID: 30429608). Briefly, end-repair, A-tailing and Illumina adapter ligation was performed using the KAPA hyper prep kit. Methylated cfDNA was immunoprecipated and purified using the Diagenode MagMeDIP and IPure kits. Prior to MeDIP, 10% of the adapter-ligated cfDNA sample was set aside as an input control. Both MeDIP and input control samples were then amplified using KAPA hyper prep kit protocols, followed by size selection.
identifiers.namespace: GEO
identifiers.id: GSM4618984