title: GSM3984860: Control rep1; Homo sapiens; ATAC-seq
library_construction_protocol: We conducted ATAC-seq as described in Buenrostro et al. (Curr. Protoc. Mol. Biol. 2015) using a home-made Tn5 that we synthesized as described in Picelli et al. (Genome Res. 2014). For each replicate we incubated 250,000 cells with 12.5 uL of 1:1 mix of Tn5 that carry 5-methylC-MEDS-A oligos and MEDS-B oligos at 37℃ for 30 minutes in a 50 uL reaction. We column-purified the tagmented DNA using the Zymo DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA). We constructed Illumina sequencing library using the Kitzman lab custom index primers. We PCR-amplified a total of 11 cycles (5 initial + 6 additional) until amplification curve reached its mid-log phase (⅓ to ½ of max signal), and then purified the products using SPRI beads prepared as in (Rohland and Reich, Genome Res. 2012) and eluted in 22 uL of TTE8 buffer.
identifiers.namespace: GEO
identifiers.id: GSM3984860