title: GSM3384568: 65E5_d0MEF; Mus musculus; RNA-Seq
library_construction_protocol: RNA was purified using TRIzol (Cat.#15596026,Invitrogen) and RNeasy mini kit (Cat#74004, Qiagen). 300 ng of RNA was used for library preparation using the KAPA stranded mRNA-seq kit (Cat#07692193001, Roche) according to the manufacturer's instructions (V5.17). KAPA single-indexed adapters (Cat#08005699001, Roche) were added to the A-tailed cDNA for library amplification with 14 cycles of PCR. The final library was purified by using Agencourt AMPure XP beads (Cat#A63881, Beckman Coulter) to size select fragments of 200-300 bp. stranded RNA-seq
identifiers.namespace: GEO
identifiers.id: GSM3384568