title: GSM3610874: Suicide_controls: P005; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Suicide_controls: Whole blood was collected from 14 subjects with no known neurological or neurodegenerative disorders as part of an ongoing longitudinal study of suicidal behavior. Blood samples were collected within PAXgene Blood ccfDNA Tubes (Qiagen). Blood plasma was separated by centrifugation for 15 minutes at 1900g and clarified with a second centrifugation for 10 minutes at 1900 x g. Cell-free DNA extraction from plasma or serum was performed using the Analytik Jena PME free-circulating DNA Extraction Kit (845-IR-0003050) using the SE/SBS system only. Bisulfite Conversions were performed using the MethylCode Bisulfite Conversion Kit (Invitrogen, MECOV50). Libaries were constructed using the Nextera transposase (Tn5) enzyme supplied within Illumina Nextera® DNA Sample Preparation Kit (FC-121-1031).
identifiers.namespace: GEO
identifiers.id: GSM3610874