title: Microbiomes of I. campana, I. sp, and surrounding bacterioplankton
design: Samples were stored in ethanol, and DNA was extracted using the Qiagen DNeasy Blood and Tissue kit.  Sequenced at Molecular Research LP (Shallowater, Texas)on a MiSeq® sequencer (Illumina).  V4 region of the 16S rRNA gene was amplified using the bacterial and archaeal primers 515F and 806R   Metadata below (formatted in for a tab delimited text file)  #SampleID BarcodeSequence LinkerPrimerSequence BarcodeName Repeats ProjectName Description Sample Source Collection Site Long &n Lat Date Collected Depth  WW1 GAGAGTGT GTGCCAGCMGCCGCGGTAA 515Fbar1  121514CM515Fillumina WW1 seawater "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WW2 GAGATCAG GTGCCAGCMGCCGCGGTAA 515Fbar2  121514CM515Fillumina WW2 seawater "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WW3 GAGATCTC GTGCCAGCMGCCGCGGTAA 515Fbar3  121514CM515Fillumina WW3 seawater "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  GW1 GAGATGAC GTGCCAGCMGCCGCGGTAA 515Fbar4  121514CM515Fillumina GW1 seawater "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GW2 GAGATGTG GTGCCAGCMGCCGCGGTAA 515Fbar5  121514CM515Fillumina GW2 seawater "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GW3 GAGTACAG GTGCCAGCMGCCGCGGTAA 515Fbar6  121514CM515Fillumina GW3 seawater "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  PW1 GAGTACTC GTGCCAGCMGCCGCGGTAA 515Fbar7  121514CM515Fillumina PW1 seawater "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PW2 GAGTAGAC GTGCCAGCMGCCGCGGTAA 515Fbar8  121514CM515Fillumina PW2 seawater "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  LW1 GAGTAGTG GTGCCAGCMGCCGCGGTAA 515Fbar9  121514CM515Fillumina LW1 seawater "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LW2 GAGTCACT GTGCCAGCMGCCGCGGTAA 515Fbar10  121514CM515Fillumina LW2 seawater "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LW3 GAGTCAGA GTGCCAGCMGCCGCGGTAA 515Fbar11  121514CM515Fillumina LW3 seawater "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  BS1 GAGTCTCA GTGCCAGCMGCCGCGGTAA 515Fbar12  121514CM515Fillumina BS1 unkown Ircinia sp "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  BS2 GAGTCTGT GTGCCAGCMGCCGCGGTAA 515Fbar13  121514CM515Fillumina BS2 unkown Ircinia sp "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  BS3 GAGTGACA GTGCCAGCMGCCGCGGTAA 515Fbar14  121514CM515Fillumina BS3 unkown Ircinia sp "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  PS1 GAGTGAGT GTGCCAGCMGCCGCGGTAA 515Fbar15  121514CM515Fillumina PS1 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PS9 GAGTGTCT GTGCCAGCMGCCGCGGTAA 515Fbar16  121514CM515Fillumina PS9 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PS3 GAGTGTGA GTGCCAGCMGCCGCGGTAA 515Fbar17  121514CM515Fillumina PS3 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PS4 GAGTTCAC GTGCCAGCMGCCGCGGTAA 515Fbar18  121514CM515Fillumina PS4 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  WS3 GAGTTCTG GTGCCAGCMGCCGCGGTAA 515Fbar19  121514CM515Fillumina WS3 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WS8 GAGTTGAG GTGCCAGCMGCCGCGGTAA 515Fbar20  121514CM515Fillumina WS8 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WS2 GAGTTGTC GTGCCAGCMGCCGCGGTAA 515Fbar21  121514CM515Fillumina WS2 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WS9 GATCACCA GTGCCAGCMGCCGCGGTAA 515Fbar22  121514CM515Fillumina WS9 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  GS10 GATCACGT GTGCCAGCMGCCGCGGTAA 515Fbar23  121514CM515Fillumina GS10 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GS1 GATCAGCT GTGCCAGCMGCCGCGGTAA 515Fbar24  121514CM515Fillumina GS1 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GS5 GATCAGGA GTGCCAGCMGCCGCGGTAA 515Fbar25  121514CM515Fillumina GS5 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GS7 GATCCAAC GTGCCAGCMGCCGCGGTAA 515Fbar26  121514CM515Fillumina GS7 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  LS8 GATCCATG GTGCCAGCMGCCGCGGTAA 515Fbar27  121514CM515Fillumina LS8 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LS4 GATCCTAG GTGCCAGCMGCCGCGGTAA 515Fbar28  121514CM515Fillumina LS4 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LS1 GATCCTTC GTGCCAGCMGCCGCGGTAA 515Fbar29  121514CM515Fillumina LS1 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LS10 GATCGAAG GTGCCAGCMGCCGCGGTAA 515Fbar30  121514CM515Fillumina LS10 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  CS8 GATCGATC GTGCCAGCMGCCGCGGTAA 515Fbar31  121514CM515Fillumina CS8 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m  CS9 GATCGTAC GTGCCAGCMGCCGCGGTAA 515Fbar32  121514CM515Fillumina CS9 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m  CS4 GATCGTTG GTGCCAGCMGCCGCGGTAA 515Fbar33  121514CM515Fillumina CS4 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m  CS10 GATCTCCT GTGCCAGCMGCCGCGGTAA 515Fbar34  121514CM515Fillumina CS10 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m
identifiers.namespace: UNCW
identifiers.id: Ircinia campana project