title: GSM3083998: Stroke_13_mRNA; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from 3ml of whole blood of 486 PREDICT patients using Tempus Spin RNA isolation kit (Invitrogen). RNA quality was determined by RNA gel for all samples and by Bioanalyser 6000 (Agilent) for samples selected for RNA-sequencing and which showed high RNA quality with a median RIN of 8.8 (lowest RIN 7.9, highest RIN 9.9). These libraries were prepared from 1000 ng total RNA using TruSeq RNA library preparation kit (Illumina). The libraries were sequenced (12 per flow cell), on the Illumina NextSeq 500 platform at the Hebrew University, Jerusalem, Israel.
identifiers.namespace: GEO
identifiers.id: GSM3083998