title: GSM4495370: Dimer_StreptavidinAP_Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the lysate upon the addition of 3 volumes of Trizol-LS (Invitrogen) and purification with the Zymo Direct-Zol kit (Zymo Scientific) according to manufacturer's instructions. Library preparation was conducted using TruSeq Stranded mRNA kit (Illumina), using a modified protocol. In lieu of polyA purification, 25 µL of washed Dynabeads MyOne C1 Streptavidin (Invitrogen) were incubated with 10 µg isolated total RNA sample in 1X RBW Buffer supplemented with 2 U/µL Murine RNAse inhibitor for 1 h at room temperature. Beads were washed 3 times in 1X RBW Buffer, followed by 2 washes with ultra-pure water. Subsequent to RNA binding and washing to the streptavidin beads, 19.5 μL of Fragment-Prime-Finish mix was added to each beads sample to elute and fragment the RNA. Library preparation was carried out in subsequent steps according to manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM4495370