title: GSM2060974: IRF8m/m_BMDM_RNA_UT_2; Mus musculus; RNA-Seq
library_construction_protocol: Independent duplicates of 5x10^5 WT, Irf8m/m and Irf1-/- BMDM were treated or not with IFNγ (400 U/ml) for 3h and RNA was extracted using RNeasy columns (Qiagen). RNA integrity was assessed on a Bioanalyzer RNA pico chip. RNA-seq libraries were prepared following poly-A enrichment using Illumina TruSeq RNA sample preparation kit. The RNA-seq libraries were sequenced on an Illumina HiSeq 2000 sequencer in paired-end 50bp configuration.
identifiers.namespace: GEO
identifiers.id: GSM2060974