title: GSM2399812: macrophages F4/80 high MHCII low, from healthy lamina propria, donor III; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Arcturus PicoPure RNA Isolation Kit (Thermo Fisher Scientific) according to manufacturer's protocol. All mouse RNAs were analyzed on Agilent Bionanlyser for quality assessment; RNA Integrity Number (RIN) range was 6.5-10 and a median of 9.2. cDNA libraries were prepared starting with 2ng of total RNA and 1ul of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion) using SMARTSeq v2 protocol (Picelli et al., 2014) except for the following modifications: 1. Use of 20µM TSO, 2. Use of 250pg of cDNA with 1/5 reaction of Illumina Nextera XT kit. The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip. All 8 samples were subjected to an indexed PE sequencing run of 2x51 cycles on an Illumina HiSeq 2500 Rapid mode (17 samples/lane).
identifiers.namespace: GEO
identifiers.id: GSM2399812