title: GSM4494925: Taipei milkripe seeds (10 DAF) rep1; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: Total RNA was prepared from approximately 100 mg of frozen, finely ground tissue with RibospinTM Seed/Fruit kit (GeneAll Biotechnology, Korea) followed by two rounds of DNase treatment (first round with RiboclearTM plus! from GeneAll, second round with TURBO DNA-free Kit from Invitrogen) and PCR was performed to assess possible remaining genomic DNA contamination. Only those total RNA samples with RIN quality scores greater than 7.3 by Bioanalyzer were submitted for RNA sequencing Library preparation and sequencing were performed at the Max Planck Genome Center, Cologne, Germany (https://mpgc.mpipz.mpg.de/home). rRNA depletion was performed on 1000 ng total RNA samples with the Ribo-Zero™ rRNA Removal Kit (seed/root) from Illumina. Subsequent library preparation was performed with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer's instructions. Quality and quantity were assessed at all steps via capillary electrophoresis (TapeStation, Agilent Technologies) and fluorometry (Qubit, Thermo Fisher Scientific).
identifiers.namespace: GEO
identifiers.id: GSM4494925