title: GSM1616441: rpoA_empty3; Pseudomonas aeruginosa; ChIP-Seq
library_construction_protocol: Pellets were lysed by treatment with 1 mg/ml lysozyme and sonicated with a microtip sonicator for 4 minutes, alternating 30 seconds on and 30 seconds off to shear DNA. Immunoprecipitation with an anti-HA antibody pre-conjugated to agarose beads or with an anti-RpoA antibody followed by protein A/G-conjugated agarose beads was carried out as descibed by Gilbert et al (Molecular microbiology 73, 1072-1085). Extracted, RNAse-treated DNA was purified using QIAquick columns and used for library construction. Libraries for ChIP Seq were constructed from the fragmented genomic DNA samples using the NEBNext ChIP Library Prep Master Mix Set for Illumina, according to the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM1616441