title: GSM1891226: WTIP; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Crosslinked cells were washed with HBS buffer, resuspended in ChIP lysis buffer (600 µl/40 ml of cell culture), flash frozen and stored at -80°C. After thawing, the cells underwent bead-beating with zirconia beads (90s) and the lysate was centrifuged for 30 minutes at 13000 rpm. The pellet was resuspended in 300 µl of ChIP lysis buffer and sonicated for 15 minutes (30s sonication/60s pause cycles). The lysate was centrifuged at 7000 rpm for 15 minutes and the antibody was added (1µg/300 µl). Samples were incubated for 1h at 4°C and then 30 µl anti-mouse antibody-coupled magnetic beads were added and samples were incubated for another 2h. After that the beads were washed and bound complexes were extracted by incubation in TE+1% SDS at 65°C for 10 minutes. DNA was precipitated from the samples using ethanol precipitation with glycogen. TruSeq
identifiers.namespace: GEO
identifiers.id: GSM1891226