title: GSM2061397: MCR:SATB2 tumor 1; Danio rerio; RNA-Seq
library_construction_protocol: Zebrafish melanomas were isolated and mechanically homogenized in RTL buffer (Qiagen) containing β-mercaptoethanol. Tumor lysates were transferred onto a QiaShredder column (Qiagen), and RNA isolation was performed using the RNA Micro Plus kit (Qiagen) according to the manufacturers instruction. Total RNA was depleted of ribosomal RNA with the Ribo-Zero Gold kit (Epicentre). Ribosome-depleted RNA was used to create multiplexed RNA-seq libraries (NEBNext Ultra), according to manufacturer's protocol
identifiers.namespace: GEO
identifiers.id: GSM2061397