title: ATM sequences from U2OS cells treated with evolved  L3-1 TALENs #2
design: Sequencing #2 from Hubbard et al, 2015.  U2OS cells were nucleofected with a plasmid expressing heterodimeric FokI fusions to evolved L3-1 TALE, together with a plasmid encoding the canonical ATM-R TALE–FokI fusion protein. After 48 h, genomic DNA was harvested and the ATM target site and its corresponding known off-target sites OffA1 and OffA23 were amplified from this genomic DNA.  The amplified sites werethen  high-throughput sequenced.  All genomic target sites (ATM on-target, OffA1 and OffA23) were combined together for this one type of TALEN treatment.
identifiers.namespace: Harvard University
identifiers.id: U2OS L3-1 TALENs #2