title: GSM4672139: P6_20umDCVC_12hrs; Homo sapiens; RNA-Seq
library_construction_protocol: Each explant was transferred to a 1.5 mL microcentrifuge tube, lysed in QIAzol reagent and homogenized in a TissueLyser LT (Qiagen) for 5 minutes at 50 Hz, using one 3.2 mm stainless steel bead per tube. mRNA was extracted using the miRNeasy Mini Kit (Qiagen) following the manufacturer's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM4672139