title: GSM4333302: HCT 116p53-/-RPL3 KO ACT D treated #3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was quantified and mixed at 10ng/µl For RNA-seq analysis, libraries were prepared according manufacturer's instructions (TruSeq RNA Sample Preparation kit, Illumina) starting from 4 μg of total RNA Libraries were sequenced by a paired-end chemistry on an NovaSeq6000 platform. Each library was loaded at a concentration of 8 pM, which was previously established as optimal. An average yield of ~4.5 Mb was obtained per sample. Quality control of library templates was performed using a High Sensitivity DNA Assay kit (Agilent Technologies) on a Bioanalyzer (Agilent Technologies). The Qubit quantification platform (Qubit 2.0 Fluorometer, Life Technologies) was used to normalise samples for the library preparation. Using multiplexing, up to 6 samples were combined into a single lane to yield sufficient coverage.
identifiers.namespace: GEO
identifiers.id: GSM4333302