title: RNA Sequencing of Vitis vinifera cv. Pedro Ximenes, sample PX-6 (project: Analysis of the origin of parthenocarpy in Corinto Bianco grapes)
design: The library was constructed from flowers of a replicate of Vitis vinifera cv. Pedro Ximenes. Inflorescences collected from different plants (individuals) were used for each replicate. The library was prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit starting from 1 ug of total RNA. The adapter-ligated library was amplified with 15 PCR cycles, followed by purification with Ampure beads. Finally, the library was run on a Bioanalyser DNA1000 chip, and quantified using a real-time PCR kit (Kapa Biosciences). Sequencing was performed on the Illumina HiSeq 2000 using v3 chemistry (flowcells and sequencing reagents).
identifiers.namespace: Instituto de Ciencias de la Vid y del Vino
identifiers.id: RNA sequencing of CV. PEDRO XIMENES, sample PX-6