title: GSM4671398: RNAseq sample 2: PAO1 Pf4 prophage knockout (P1); Pseudomonas aeruginosa PAO1; RNA-Seq
library_construction_protocol: The biofilm was treated with RNAprotect Bacteria Reagent (Qiagen) before RNA was extracted using the RNeasy Mini Kit (Qiagen) as per manufacturer's protocol. Contaminating DNA was removed using TURBO Dnase (Ambion) and purified using the RNA Clean & Concentrator (Zymo Research) The total RNA was converted to double stranded cDNA and made into libraries using the TruSeq Stranded mRNA Sample Preparation as per manufacturer's instruction but without the oligo dT purification. The sequencing was done using the HiSeq Rapid SBS Kit V2 200 cycles.
identifiers.namespace: GEO
identifiers.id: GSM4671398