title: Bacteria 16S amplicons Pocillopora spp. coral: adult whole tissue
design: Total DNA (coral, algal, and microbial DNA) was extracted using the Qiagen DNeasy PowerSoil kit. PCR amplification of the V4 region of the bacterial 16S gene was completed with the PCR primers 515F and 806RB with single-indexed Illumina adapters. PCR amplicons were checked for quality and size distribution on a 1.5% TE-agarose gel, and amplicon DNA concentration was determined using a Qubit fluorimeter and Qubit dsDNA BR kit. amplicons from all samples and controls were purified using AMPure XP beads and 80% ethanol washes to remove contaminants, then final DNA amplicon products were normalized to 4 nM concentration and pooled before 300 bp paired-end sequencing on the Illumina MiSeq instrument.
identifiers.namespace: SUB6509979
identifiers.id: Ot1-3b