title: WT-DT 3 sample from 16S amplicon sequencing of mouse stool
design: Amplicon sequencing of mouse stool gDNA using V4-V5 primers. IL-21eGFP transgenic mice, carrying a DTR-GFP gene under IL-21 gene regulatory sequences, were treated with diphtheria toxin (DT) from 3-6 weeks of age to determine the effect of ablating the GFP+ T cells. Control PBS treated IL-21eGFP mice, PBS treated WT mice, and DT treated WT mice were also included in the study. The composition of the fecal microbiota was determine at the end of the experiment. N= 6 mice per group. The samples were processed using 16S amplicon sequencing on Mi-seq sequencer with in-house customised primers to V4-V5 regions.
identifiers.namespace: SIgN
identifiers.id: WT-DT 3