title: GSM2828444: P4; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed twice with cold PBS, trypsinized and normalized cell numbers; extract total RNA and normalize to 1ug/ul. Goat anti-Mouse IgG beads (50ul) were incubated with anti-BrU (2ug), wash with PBS+0.1% BSA, and resuspend in 200ul. 200ul prepaired RNA were heated at 80C for 10min to open secondary structure, and incubate with 200ul antibody-bound beads for 1 h at room temperature. Wash 3 times with PBS+0.1% BSA. Extract beads captured RNA with RNeasy MiniElute Cleanup kit (QIAGEN). RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2828444