title: GSM3609624: KDM1A KO rep3: MVBG413KO; Mus musculus; RNA-Seq
library_construction_protocol: Testes were removed and decapsulated. They were then digested with collagenase B (2mg/mL) for 10 minutes at 37°C. After rinsing in PBS, another digestion with 0.25% trypsin-EDTA for 5 minutes at 37°C was performed. The resulting cell suspension was washed twice, resuspended in sterile phosphate buffer solution (PBS) with DNase (50μg/mL) and filtered (40μm). Spermatogonia were isolated by fluorescence-activated cell sorting of Oct4-GFP positive germ cells with a FACSAria III laser sorter. Total RNAs from control and Kdm1a-cKO isolated PND 6 spermatogonia (n = 4 for each group) were extracted using the PicoPure RNA isolation kit (Arcturus, Burlington, ON, Canada). A total of 250 ng of each sample was used to prepare libraries using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM3609624