title: GSM2405862: TP1-BS-seq; Sus scrofa; Bisulfite-Seq
library_construction_protocol: DNA of each collected tissue was extracted using DNeasy Blood & Tissue Kit (Qiagen). A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer’s instructions. Then these DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research). And the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X).The library preparations were sequenced on an Illumina Hiseq 2000 platform
identifiers.namespace: GEO
identifiers.id: GSM2405862