title: GSM4121468: Sample_TH1+; Mus musculus; RNA-Seq
library_construction_protocol: For each replicate (3 replicates per population) to be used in RNA-Seq, samples from several sorts were pooled. The mRNA was isolated using magnetic beads-based poly(A) selection, fragmented, and randomly primed for reverse transcription These cDNA fragments were then end-repaired, added with a single 'A' base, and ligated to Illumina® Paired-End sequencing adapters. The products were purified and PCR-amplified to create the final cDNA library. The libraries from individual samples were pooled in groups of four for cluster generation on the Illumina® cBot using Illumina® TruSeq® Paired-End Cluster Kit
identifiers.namespace: GEO
identifiers.id: GSM4121468