title: Illumina HiSeq 2000 sequencing; RNA-seq study of sexual reproduction in the diatom Skeletonema marinoi induced by a change in salinity
design: RNA-seq study of sexual reproduction in the diatom Skeletonema marinoi induced by a change in salinity
library_construction_protocol: Cultures were collected by filtration onto 1.2 µm pore-size membrane filters (RAWP04700 Millipore) RNA was extracted with Trizol™ (Invitrogen) according to manufacturer's instructions Single-end (SE) libraries were prepared using a Beckman Biomek FX and the Illumina® TruSeq® Stranded Total RNA Sample Preparation kit, with poly-A selection and starting with 500 ng total RNA.
identifiers.namespace: Stazione Zoologica Anton Dohrn of Naples
identifiers.id: E-MTAB-8091:NoCL16_T2_s