title: WW3_mlss_1
design: Triplicate PCR reactions was performed in a 25–μl reaction volume using the HotStarTaq Plus Master Mix (QIAGEN, Valencia, CA), 0.5 µM of each primer and 100–200 ng of template DNA and amplified using 8F (5'–AGAGTTTGATCCTGGCTCAG–3') and 533R (5'–TTACCGCGGCTGCTGGCAC–3') with the following PCR conditions: 95-5min, 25X(95-30s,55-30s,72-30s),72-5min using a C1000 Thermal Cycler (BIO-RAD, Hercules, CA).
identifiers.namespace: King Abdullah University of Science and Technology
identifiers.id: MBR3.AS.1