title: GSM4064452: 3_24E [miRNA]; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was extracted from TRIzol Reagent-lysed ARPE-19 cells, according to the manufacturer's instructions, and stored at -80 °C ahead of use for RNA-seq. RNA concentration was determined by spectrophotometry on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific-Invitrogen) for RNA-seq. RNA integrity was confirmed on the 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). Small RNA was selectively enriched from total RNA using the TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA). The library was sequenced on Illumina NextSeq 500, using NextSeq 75-cycle High Output Kits (Illumina), with the PhiX Control v3 library (Illumina) as sequencing control.
identifiers.namespace: GEO
identifiers.id: GSM4064452