title: GSM2061357: GH671; TCAB1kd; U1; Homo sapiens; OTHER
library_construction_protocol: Cultured HeLa cells were cross-linked in 2% formaldehyde at 37°C for 10min. After being quenched with glycine, cells were lysed using 10mM Tris-HCl, pH8.0, 10mM NaCl, 0.2% NP-40) supplemented with 1× complete protease inhibitors (Sigma) at 4°C for 1 h. Cross-linked chromatin was digested overnight with an excess of HindIII enzyme (New England Biolabs), and then DNA ends were ligated under diluted conditions that favor junctions between cross-linked DNA fragments. NlaIII (New England Biolabs) was used in a second round of digestion, and DNA was re-ligated. 4C DNA samples were barcoded and sequenced on Illumina HiSeq 2000 according to the Illumina paired-end 100 bp sequencing protocol.
identifiers.namespace: GEO
identifiers.id: GSM2061357