title: GSM3986112: BL8_V_IP_PBS; Mus musculus; RNA-Seq
library_construction_protocol: After homogenization RNA was purified from (i) cleared lysate as the input, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag. LPS samples were compared to PBS samples. PFC samples were compared to VCX samples. Input and IP samples underwent DNA depletion and RNA purification with the RNeasy Micro Plus kit (Qiagen). Integrity of the eluted RNA was analyzed by a 2100 Bioanalyzer (Agilent). Sequencing libraries were prepared using Illumina TruSeq RNA sample prep kit following manufacturer's protocol.  After library preparation, amplified double-stranded cDNA was fragmented into 125 bp (Covaris-S2, Woburn, MA) DNA fragments, which were (200 ng) end-repaired to generate blunt ends with 5'- phosphates and 3'- hydroxyls and adapters ligated.
identifiers.namespace: GEO
identifiers.id: GSM3986112