title: GSM4061344: Stim_Rep3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from cell pellets using the Qiagen RNeasy Mini kit according to the manufacturer's instructions. First-strand Illumina-barcoded libraries were then generated using the NEB RNA Ultra Directional kit according to manufacturer's instructions, including 12-cycles of PCR enrichment. Libraries were constructed using the NEB Ultra-Directional RNA kit using the mRNA magnetic isolation module, according to manufacturer's instructions. Directional libraries were barcoded using NEB dual index barcodes and sequenced on the NextSeq (high output flowcell) by pair-end sequencing of 37bp.
identifiers.namespace: GEO
identifiers.id: GSM4061344