title: GSM4220503: WT_CB2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using Qiagen RNeasy micro kit, following the manufacturer protocol. Briefly, samples were lysed in ± 300l RLT buffer supplemented with 400mM DTT by repeated vortexing (5 times, 4 seconds on low speed). Supernatants were kept and supplemented with 70% ethanol to reach 150% of recovered volume. Samples were loaded on columns and manufacturer protocol was followed. DNase treatment was conducted on-columns for 10 minutes. Elution of RNA from the columns was done using 13ul DNase/RNase free water. TrueSeq Stranded RNA kit with RiboZero (Illumina )
identifiers.namespace: GEO
identifiers.id: GSM4220503