title: GSM1618376: 5hmC.Naive; Mus musculus; MeDIP-Seq
library_construction_protocol: the genomic DNA was purified and sonicated. DNA fragments (4 ug) were denatured and incubated with antibodies against 5mC (Eurogentec) and 5hmC (Active Motif) at 4 degree overnight. Antibody-DNA complexes were captured by protein A agarose/Salmon sperm DNA. The immunoprecipitated DNA was purified. The results were normalized relative to the input control. Libraries were prepared using Illumina ChIP-seq DNA preparation kit (IP-202-1012) according to the manufacture's instruction.
identifiers.namespace: GEO
identifiers.id: GSM1618376