title: GSM3929160: Wheat_ATAC_S1_001; Triticum aestivum; ATAC-seq
library_construction_protocol: 100 mg of 14-d-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM beta-mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63µm. DNA libraries were amplified for a total of 8 cycles as described by Buenrostro et al., (2013) and Jégu et al., (2017). 100 mg of 14-d-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM beta-mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63µm. Nuclei were resuspended in 1X TD Buffer (Illumina FC-121-1030) and 2.5 μL of Tn5 Transposes (Illumina FC-121-1030) were added. Transposition reaction was performed at 37°C for 30min, and DNA was purified using a Qiagen MinElute Kit. DNA libraries were amplified for a total of 8 cycles as described by Buenrostro et al., (2013) and Jégu et al., (2017).
identifiers.namespace: GEO
identifiers.id: GSM3929160