title: GSM4671320: MT2_scRNAseq_Day1_DMSO; Mus musculus; RNA-Seq
library_construction_protocol: All RNA was extracted using Qiagen's RNeasy kit following manufacturer's protocol.   DNA was isolated by phenol:chloroform: isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR purification. At least 1ng of ChIP DNA was used to prepare sequencing library with NEBUltra ChIP Sample Library Prep Kit (New England Biolabs).    For bulk RNA-seq Indexed polyA libraries were prepared using 200 ng of total RNA and 14-15 cycles of amplification with the Agilent SureSelect Automated Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing (G9691B) on an Agilent NGS Workstation Option B liquid handling platform (G5522A).    For scRNA-seq, amplified cDNA was prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v2 (10x Genomics, PN-120237) with 9-13 cycles of amplification according to manufacturer's instructions with modifications specified in “TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v2” protocol (BioLegend). Sequencing libraries were prepared from the cDNA fraction according to the 10x Genomics protocol using 11 cycles of amplification. The HTO sequencing library was prepared from the remaining fraction according to the TotalSeq-A protocol using 8 cycles of amplification.
identifiers.namespace: GEO
identifiers.id: GSM4671320