title: GSM4622604: 729MIR; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA is extracted from control or mimic-treated OA FLS (n=3 per group) by standard Trizol method and quantified using the Qubit RNA BR assay (Denovix). Quality of RNA is assessed using the Agilent RNA Nano chips on a Bioanalyzer instrument. For all the samples, RNA integrity number (RIN) was >9.7. Total RNA libraries are prepared from 200 ηg extracted RNA using the TruSeq Stranded Total RNA Library Preparation kit with RiboZero (Illumina Cat #20020596) according to manufacturer's protocol. Individual libraries are quantified using a fluorometric high-sensitivity dsDNA assay (Denovix). Library quality is assessed on a high sensitivity DNA chip on the Agilent 2100 Bioanalyzer system (Agilent). Libraries are prepared for sequencing (volumetrically pooled, diluted and denatured): 75 paired end read-cycles on an Illumina NextSeq 550 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM4622604