title: GSM4278332: Wheezer sample 14; Homo sapiens; RNA-Seq
library_construction_protocol: AMs were bead sorted and immediately lysed in TRIZol reagent. Lysates were mixed 5:1 with chloroform and centrifuged at 10 000 g for 10 minutes at 4oC to obtain RNA in top aqueous layers. Aqueous layers were then mixed with an equal volume of 70 % ethanol and RNA extracted using the RNeasy Mini Kit from QIAGEN with on-column DNase digestion, as per manufacturer's instructions. 200 ng RNA per sample was polyA-purified and converted to cDNA sequencing libraries using the Illumina TruSeq Stranded mRNA v2 kit, with unique indexes per sample to allow multiplexing.
identifiers.namespace: GEO
identifiers.id: GSM4278332