title: GSM2402716: Repo/TDP43:S22_small RNA; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: All small RNA libraries were constructed from 15-20 ug of TRIzol isolated total RNA. 18-29 nucleotide long small RNAs were size selected on 15% PAGE-UREA gel. After 3’- and 5’-adapter ligation, subsequent gel purification steps and reverse transcription cDNAs were PCR amplified with barcoded primers. PCR products were size selected on 6% PAGE gel, and quantified on Bioanalyzer. Loading concentrations were determined using the NEBNext Library Quant kit for Illumina (NEB). The libraries were sequenced on illumina NextSeq 500 platform.
identifiers.namespace: GEO
identifiers.id: GSM2402716