title: GSM1891380: 2013_024_006 [non pathological renal tissue]; Homo sapiens; miRNA-Seq
library_construction_protocol: Fresh-frozen tissue samples (approximately 50 mg) were cut on liquid nitrogen and homogenized with Yttrium stabilized Zirkonoxid-Beads (Silibeads, Sigmund-Lindner, Warmensteinach, Germany) using a Precellys24 tissue homogenizer. Total RNA was isolated using the mirVana miRNA Isolation Kit (Ambion, Foster City, CA, USA) according to the manufacturers recommendation. To remove residual DNA fragments, we treated the isolate twice with DNase (DNA-free Kit, Ambion). Libraries for small RNA sequencing were prepared using the TruSeq small RNA library kit (Illumina) according to the manufacturer’s instructions. 500 ng of total RNA was used as input for RNA adapter ligation, followed by reverse transcription and PCR amplification with bar-coded primers. Small RNA libraries were sequenced on a NextSeq500 (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM1891380