title: 16S H20 French Creek
design: Samples stored in DNA storage buffer on 0.2m polycarbonate filters, followed by DNA extraction with EZNA Kit (See Github for exact protocols). DNA was amplified via PCR for V6-V8 region of 16S rRNA gene (primers: B969F and BA1406R). Samples were cleaned with Agencourt AMPure XP PCR purification kit. Illumina kit used was 2x300bp v3 chemistry with 10% PhiX spike.
identifiers.namespace: SUB5358937
identifiers.id: H2O_054_508_719