title: GSM4176206: X11_WT_YS_MAC1; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were removed, cell were FACS sorted and 20,000-100,000cells were collected into TRIzol and frozen at -80°C till RNA extraction. TPhase separation was achieved with the addition of chloroform (Sigma), and total RNA was precipitated from the aqueous layer with isopropanol (Sigma) using glycogen (Roche) as a carrier The TruSeq RNA Stranded sample kit (Illumina) was used to construct the sequencing libraries
identifiers.namespace: GEO
identifiers.id: GSM4176206