title: GSM1942776: GPR65-KO-IL1B_IL6_IL23-96h (population, rep2); Mus musculus; RNA-Seq
library_construction_protocol: Cells were washed, stained and sorted for CD3 (Biolegend), CD4 (Biolegend), 7AAD and IL-17A-GFP+. population controls were generated by extracting total RNA using a RNeasy plus Micro RNA kit (Qiagen) according to manufacturer’s recommendations. Subsequently, 1 μL of RNA in water was added to 2  μL of lysis reaction mix, thermocycled using cycling conditions I described in Supplementary Experimental Procedures in corresponding publication. Next, 4  μL of the RT Reaction Mix were added, and the mixture was thermocycled using cycling conditions II. Finally, 1  μL of the total RT reaction was added to 9 μL of PCR mix, and that mixture was thermocycled using cycling conditions III. Products were quantified, diluted to 0.125 ng/ μL, and libraries were prepared, cleaned, and tested as described in Supplementary Experimental Procedures in corresponding publication.
identifiers.namespace: GEO
identifiers.id: GSM1942776