title: GSM4221273: ES-2 AZ_1000:2_250:7 MKG15; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were grown in 25cm2 flasks until they reached 70% confluency then total RNA was extracted using the RNeasy mini kit (74104, Qiagen Ltd., Manchester, UK) according to the manufacturers' instructions. RNA was quantified and DNA contamination assessed using Qubit RNA HS and dsDNA HS assay kits (Thermo Fisher Scientific) on a Qubit 2.0 Fluorimeter. Samples were checked for RNA integrity with the RNA 6000 Nano Kit (Agilent Technologies UK Ltd., Edinburgh, UK) using an Agilent 2100 Bioanalyser. RIN numbers for all samples were >9.6, well in excess of the 8.0 quality threshold for RNA-Seq, which was performed at the Wellcome Trust Clinical Research Facility, Western General Hospital, Edinburgh. 500ng of each RNA sample was processed for total RNA library preparation using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (Illumina, Cambridge, UK) according to the provided protocol
identifiers.namespace: GEO
identifiers.id: GSM4221273