title: GSM2828812: RNA-seq from Senescent Fibroblasts (p300 knockdown shRNA #1), Replicate #2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from frozen pellets of IMR90 cells using the RNeasy kit (Qiagen) following instructions. 1ug was used as input in the Scriptseq Complete kit (Epicentre). Briefly, total RNA was ribodepleted using a Ribozero magnetic protocol for human, mouse and rat (Epicentre, Illumina). The ribodepleted RNA was then ethanol precipitated, fragmented, and tagged at both ends for stranded library preparation using the Scriptseq v2 library preparation protocol. The PCR amplification step was used to index the libraries and multiplexed libraries were quantified by Bioanalyzer (Agilent) and qPCR (Kapa Biosystems). The RNA-seq run was performed on the NextSeq 500 platform (Illumina). RNA-seq libraries were sequenced using an Illumina NextSeq 500 platform (only forward reads in the paired-end sequencing were used, 48bp).
identifiers.namespace: GEO
identifiers.id: GSM2828812