title: GSM3539207: Bmal1-/-2_T12_rep2 [run1_VAL513A20_S20]; Mus musculus; RNA-Seq
library_construction_protocol: Wild-type and Bmal1-/- MSF cells and mice liver tissue time-course samples were lysed in TRI-Reagent® and Direct-zol™ RNA MiniPrep kit was used for RNA extraction following the manufacturer's instructions (Zymo Research; Cat # R2052). mRNA contents in those samples were enriched using a NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB; Cat # E7490). The enriched mRNA samples were subjected to an Illumina TrueSeq platform compatible multiplex library preparation protocol using KAPA Stranded RNA-Seq Library Preparation Kit (Kapa Biosystems; Cat # KK8401) and libraries were prepared with TruSeq LT index adapter.
identifiers.namespace: GEO
identifiers.id: GSM3539207