title: GSM4332997: HG1; Oreochromis niloticus; miRNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure Small RNA enrichment was done by excision of the 15 to 50 nt fraction from a polyacrylamide gel. Approximately 1 ug of enriched RNA were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA). And then we performed the single-end sequencing (36bp or 50bp ) on an Illumina Hiseq 2500 at the LC Sciences  following the vendor's recommended protocol.
identifiers.namespace: GEO
identifiers.id: GSM4332997