title: GSM2566888: Exp 1 Treated rep 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from the dissected mouse tissues using TRI-reagent (Sigma), following the manufacturer’s instructions, which included a chloroform phase separation and ethanol precipitation. RNA was resuspended in 70 μL of RNase-free water. RNA samples were assessed for quantity and quality using a NanoDrop UV spectrophotometer (Thermo Fisher Scientific Inc), conventional RNA electrophoresis and using a Bioanalyser (Agilent Technology Inc). All RNA integrity numbers (RIN) for the analysed samples ranged from 9 to 9.5 mRNA libraries were prepared at the Ramaciotti Centre for Genomics (UNSW University of New South Wales, Australia). The Illumina TruSeq RNA Prep Kit was used for Experiment 1. TThe six RNA-Seq libraries in Experiment 1 were sequenced on the Illumina HiSeq2000 platform at the Ramaciotti Centre for Genomics UNSW. R1.fastq and R2.fastq files were produced for each sample.
identifiers.namespace: GEO
identifiers.id: GSM2566888