title: GSM4221343: SOX18(AOGG-85); Homo sapiens; RNA-Seq
library_construction_protocol: Cultured ECs were lysed using 350 μL RLT lysis buffer (Qiagen) with β-mercaptoethanol (1%) and subsequently vortexed for 1 min at room temperature before snap freezing. RNA was isolated from cell lysates using an automated Maxwell Total RNA purification kit (Promega) with DNAse I digestion. Total RNA was subjected to oligo (dT) capture and enrichment, and the resulting mRNA fraction was used to construct cDNA libraries. Sequencing was performed on an Illumina HiSeq 4000 platform using the standard protocol (TruSeq Stranded Total RNA Library, Illumina) that generates approximately 30 million reads of 50 base pairs per sample.
identifiers.namespace: GEO
identifiers.id: GSM4221343