title: 16S Miseq rRNA of rat microbiota
design: The bacterial V3-V4 region of the 16S ribosomal gene was amplified from purified genomic DNA with the primers F343-2 (5 CTTTCCCTACACGACGCTCTTCCGATCTACGGGWGGCAGCAG) and R784-2 (5 GGAGTTCAGACGTGTGCTCTTCCGATCTTACHRGGGTATCTAATCC) using 30 amplification cycles with an annealing temperature of 55 C.
identifiers.namespace: SUB3681173
identifiers.id: 9_control3