title: GSM4672311: B3_16 month old C57BL/6; Mus musculus; RNA-Seq
library_construction_protocol: Mice were deeply anesthetized by intraperitoneal injection of 50% chloral hydrate in PBS (pH 7.4; 2.5 mg chloral hydrate per g body weight). Adult cortical hemispheres were dissected from whole brain and frozen in liquid nitrogen. Samples were subjected to standard RNA isolation procedure using Trizol Reagent (Life Technologies, United States). The TruSeq RNA Sample Preparation Kit (Illumina, Cat. No RS-122-2002, United States) was used for library preparation (1 mg total RNA), the QuantiFluorTM dsDNA System (Promega, United States) for quantitation and the DNA 1000 chip on the Bioanalyzer 2100 (Agilent Technologies) to determine the size range of final cDNA libraries prior to amplification and sequencing (cBot and HiSeq2000 from Illumina; PE; 2 100 bp; ca. 30 million reads per sample).
identifiers.namespace: GEO
identifiers.id: GSM4672311