title: GSM1940150: K562 PU.1 shRNA2 SAHA DNase-seq Rep 1; Homo sapiens; DNase-Hypersensitivity
library_construction_protocol: 30-50 million nuclei were digested with a range of recombinant DNase I enzyme (Roche) concentrations (between 1.2 and 12U) for 15 minutes at 37°C in 120uL 1X DNase buffer. Libraries constructed according to PMID: 20150171 Cell pellets were washed twice with 1xPBS and nuclei isolated by 0.1% NP-40 lysis
identifiers.namespace: GEO
identifiers.id: GSM1940150