title: GSM1698479: cyto_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from pooled technical triplicate sucrose gradient fractions by ethanol precipitation followed by acid phenol:chloroform extraction.  Direct phenol:chloroform extraction was precluded by phase inversion in high sucrose fractions. RNA was then DNase treated, subjected to a second acid phenol:chloroform extraction, and ethanol precipitated. Cytoplasmic RNA was extracted using TRIzol extraction (Life Technologies) and ethanol precipitation. Total RNA integrity was verified using a BioAnalyzer (Agilent). Ribosomal RNA was then depleted using Ribo-Zero (Illumina) and biological duplicate sequencing libraries were generated using the TruSeq RNA Sample Prep v2 kit (Illumina) without the poly-A selection steps.  An equal mass of rRNA-depleted RNA was used as input to each individual library preparation.
identifiers.namespace: GEO
identifiers.id: GSM1698479