title: GSM2481057: Ribosome Profiling METTL3 KD2 Day 8; Homo sapiens; OTHER
library_construction_protocol: 5*10^7 MOLM13 CTRL or METTL3 KD cells at day 5 or day 8 after doxycycline induction were treated with 0.1 mg/ml of cycloheximide for 1 minute and the ribosome protected fragment (RPF) fraction of mRNA or total input control mRNA was isolated using the RNeasy Mini Kit (Quiagen) following the manufacturer instruction. Ribosomal RNA was removed using the Illumina Ribozero Kit. Single end 50 bp stranded libraries were prepared using the Illumina TRUseq Ribo Profile kit following manufacturer's recommendations. Reads were sequenced using HiSeq 1500 and multiplexed reads were split based on their barcodes using Illumina Basespace.
identifiers.namespace: GEO
identifiers.id: GSM2481057