title: GSM4672479: CTCF knockout biological replicate 1 RRBS; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA from left ventricular isolated adult cardiomyocytes was isolated using the QIAshredder kit (Qiagen). Initial digestion into CpG rich regions was done by the Msp1 enzyme (NEB). The 3' → 5' Klenow exonuclease was used to repair sticky ends and promote poly-A tail synthesis. To facilitate multiplexing, individual TruSeq Illumina adapters were ligated to samples using the QIAseq Methyl Library Kit (Qiagen). Samples were then filtered to enrich for DNA fragments within the desired size range using magnetic AMPure XP beads (Beckman). Bisulfite conversion was performed using the Zymo EZ DNA Methylation-Lightning kit (Zymo).
identifiers.namespace: GEO
identifiers.id: GSM4672479