title: GSM2904944: Root_RNAseq; Scutellaria viscidula; RNA-Seq
library_construction_protocol: Total RNA was extracted from each collected tissues using CTAB RNA isolation method (Chang et al. 1993). Three replicates were sampled and mixed for RNA preparation. RNA samples were treated with RNase-free DNase (TaKaRa, Dalian, China) to eliminate genomic DNA contamination. Degradation of RNA was monitored on 1% agarose gels. Purity of RNA was checked using Nano Photometer spectrophotometer (Implen, CA, USA). The concentration of RNA was measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA). The integrity of RNA was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total of 16.5 μg RNA was equally pooled from the collected tissues for cDNA library preparation. Library quality assessment, assembling, clustering and sequencing were performed following the method of Chen et al. (Chen et al. 2014). RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM2904944