title: GSM1700531: WT_SK1_2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA was extracted using standard hot phenol procedure. rRNAs were depleted from total RNA using the RiboMinus Eukaryote System v2 (Life Technologies). Alternatively, total RNA was treated with Terminator 5'-Phosphate-Dependent Exonuclease (Epicentre) to digest all 5'-monophosphate-ended RNAs. Strand-specific total RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina), starting from 125 ng of rRNA-depleted RNA or Terminator-treated RNA . Paired-end sequencing (2x50 nt) of the libraries was performed on a HiSeq 2500 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM1700531