title: GSM4671330: RPL34-AS1-NC-2; Homo sapiens; RNA-Seq
library_construction_protocol: fresh frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. After the extracted total RNA sample is subjected to agarose electrophoresis and Nanodrop quality inspection and quantification, oligo (dT) magnetic beads are used to enrich mRNA (if the RNA is a degraded sample or a prokaryotic sample, it is directly processed by rRNA removal kit); RNA sequencing The library is completed by the kit, including RNA fragmentation and inversion of random primers to generate first-strand cDNA, adding dUTP to synthesize the second-strand cDNA, double-stranded cDNA end repair plus A connected to Illumina matching adapter, PCR amplification to obtain the final library ; The constructed library is quality checked with Agilent 2100, and the library is quantified by qPCR method, and sequenced using Illumina NovaSeq 6000 sequencer RNA libraries were prepared for sequencing using standard Illumina NovaSeq 6000.
identifiers.namespace: GEO
identifiers.id: GSM4671330