title: GSM4221102: mRNA levels in Cap-G knockdown (with nSyb-GAL4) 3rd instar brains_rep3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For Targeted DamID experiments, genomic DNA was isolated,RNAseA treated and DpnI digested. DamID adaptors were ligated to DpnI digested DNA followed by DpnII digestion PCR amplification, sonication and removal of DamID adaptors (Marshall et al., 2016). For RNA-seq experiments, total RNA was extracted from dissected 3rd instar larvae CNS, 35 per replicate, using a standard TRIzol extraction protocol. RNA libraries and sequencing was performed by BGI (www.bgi.com). Targeted DamID libraries were generated using the Illumina Truseq Library Kit. RNA-seq libraries were generated by BGI (www.bgi.com).
identifiers.namespace: GEO
identifiers.id: GSM4221102