title: GSM2829313: HoxB7cre_HET_SCR17; Mus musculus; RNA-Seq
library_construction_protocol: RNA from microdissected kidneys was extracted by Tryzol using the miRNA mini kit Qiagen for extraction of total and miRNAs. The quality of the RNA samples was assessed on the Agilent Bioanalyzer system using the Agilent RNA 6000 Nano Kit  (Agilent Technologies) and RNA with a Ring higher than 8 was used. 1-2 μg of total RNA were used for mRNA isolation using the Dynabeads® mRNA DIRECT™ Micro Kit (ThermoFisher Scientific). mRNA was digested with RNase III, purified, hybridized and ligated to Ion Adaptors, reverse transcribed, barcoded and amplified, using the Ion Total RNA‐Seq Kit v2 (ThermoFisher Scientific).
identifiers.namespace: GEO
identifiers.id: GSM2829313