title: GSM4125332: W22_Elicited_72h; Zea mays; RNA-Seq
library_construction_protocol: Total RNA was isolated with the NucleoSpin® RNA Plant Kit (Takara Bio USA) according to the manufacturer's protocol. RNA quality was assessed based on RNA integrity number (RIN) using an Agilent Bioanalyzer. The mRNA was enriched from total RNA using oligo (dT) magnetic beads. The mRNA was fragmented randomly into short sequences followed by first-strand cDNA synthesis with random hexamer-primed reverse transcription. Second-strand cDNA synthesis was done by nick-translation using RNaseH and DNA polymerase I. After adaptor end-repair and ligation, cDNA was amplified via PCR and purified to create the final cDNA library. cDNA concentration was quantified using a Qubit 2.0 fluorometer (Life Technologies) and then diluted to 1 ng/µL before assessing insert size on an Agilent Bioanalyzer 2100.
identifiers.namespace: GEO
identifiers.id: GSM4125332