title: GSM3537275: iAD mouse negative Dox; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from iPAD and iPD mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. Uteri from iAD mice were harvested and subjected to epithelial cells isolation using Multi Tissue Dissociation Kit 1, CD45 MicroBeads, and CD326 MicroBeads (all from Miltenyi Biotech) according to manufacturer protocols. Total RNA from the isolated iAD mice uterine epithelial cells were extracted using the Qiagen RNeasy Plus Micro Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8 for iPD and iPAD mice uteri, and 7.0 to 9.4 for iAD mice uterine epithelial cells. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x150 bp paired-end high output V4 chemistry configuration.
identifiers.namespace: GEO
identifiers.id: GSM3537275