title: GSM4220781: Yap1_Rep3; Gallus gallus; OTHER
library_construction_protocol: Dorsal neural tubes were dissected from HH9+ (8ss) embryos, and a total of 20 neural folds were used/sample for CUT&RUN experiment. Cells were dissociated in Accumax (Accutase, SCR006) cell dissociation solution for 20 minutes at RT under mild agitation. CUT&RUN was perfromed as described by Skene and Henikoff, 2017. CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) using manufactures protocol. The libraries were sequenced with paired-end 37bp reads on an Illumina NextSeq500 instrument. CUT&RUN
identifiers.namespace: GEO
identifiers.id: GSM4220781