title: GSM3684699: Young.SD6hr.66976.D5; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized, and brains were removed and stored at -80°C. Microdissection of mPFC was performed using micropunch. RNA extraction was performed using an RNAqueous-Micro Kit and genome DNA was removed with RNase-Free DNase. Total RNA from each sample was prepared for sequencing using the Illumina: TruSeq Stranded mRNA Sample Preparation Kit. 200 ng of RNA was used to prepare the RNA libraries. The libraries were sequenced on Illumina HiSeq 4000. Depths of 19-32 million single 100bp reads were generated for each sample.
identifiers.namespace: GEO
identifiers.id: GSM3684699