title: Transciptome profiling of ovarian cancer cell lines
design: Transciptome profiling of ovarian cancer cell lines
library_construction_protocol: Cell lines were grown in RPMI-1640 + 10% fetal bovine serum + 1x penicillin/streptomycin (all Invitrogen, Paisley, UK) at 37 1C, 5% CO2. RNA was extracted in triplicate from confluent T75s using TRIzol reagent (Invitrogen). Extracted RNA was pooled and DNase treated using the DNAfree kit (Ambion). mRNA was then enriched by binding to oligo dT beads using the uMACS system (Miltenyi Biotech). First and second strand cDNA synthesis were carried out using the Superscript double stranded cDNA synthesis kit (Invitrogen). Half of each cDNA reaction was used for sonication and library preparation.
identifiers.namespace: CRI
identifiers.id: E-MTAB-691:PEO23_090316_HWI-EAS202_130_30F2BAAXX_s_7