title: GSM1698087: S2_RNA_Seq_replicate_1_L002; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA from Drosophila S2 cells was extracted using Promega's SV Total RNA Isolation System (Z3100) according to manufacturer’s protocol. The Illumina® mRNA-Seq Sample Prep Kit was used to process the sample(s). The sample preparation was performed according the Illumina protocol "Preparing Samples for Sequencing of mRNA" (1004898 Rev. D). Briefly, mRNA was isolated from total RNA using the poly-T-oligo-attached magnetic beads. After fragementation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip (see appendices). The size of the resulting products was consistent with the expected size of approximately 300 bp. Clustering and DNA sequencing using the Illumina HiSeq 2000 (Solexa) were performed according manufacturer’s protocols. A total of 6.5 pmol of DNA was used. Two sequencing reads of 100 cycles each using the Read 1 sequencing and Read 2 sequencing primers were performed with the flow cell.
identifiers.namespace: GEO
identifiers.id: GSM1698087