title: GSM1890767: ICE_ko24; Mus musculus; OTHER
library_construction_protocol: Cells were fixed in 2% formaldehyde for 10 min at room temperature, quenched with 125 mM glycine, washed twice with PBS, and nuclei were prepared and frozen as a dry pellet in liquid nitrogen. Nuclei were digested with EcoRI overnight at 37°C, then diluted 10 times and ligated at least 4hrs at 16°C. Extracted DNA was digested with DpnII and purified, and then ligated in 15 ml for at least 4hrs at 16°C. After precipitation, DNA was amplified by PCR using a forward primer containing the P5 Illumina adaptor sequence followed by a 4 base tag and an EcoRI site specific sequence, and a reverse primer composed of the P7 illumina adapter sequence followed by a DpnII site specific sequence. Products were verified on gel for linearity and reproducibility.
identifiers.namespace: GEO
identifiers.id: GSM1890767