title: GSM3009673: Transformed HMECs rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was obtained from cells using the TRIsure reagent (Bioline, Luckenwalde Germany) according to the manufacturer's protocol. RNA quantity was analyzed using a NanoDrop spectrophotometer, and ribosomal RNA was depleted using the Ribozero Gold kit (Epicentre, Madison, WI, USA). 50ng of rRNA depleted RNA was used as input for sequencing libraries, which were made using the ScriptSeq v2 RNA-seq library preparation kit (Epicentre) according to the manufacturer's instructions. 16 amplification cycles were used in the final PCR amplification step.  Barcodes: Prim rep 1: GATCAG; Prim rep 2: CAGATC; Prim rep 3: ACTTGA; Imo rep 1: TAGCTT; Imo rep 2: ATCACG; Imo rep 3: CGATGT; Tfo rep 1: GGCTAC; Tfo rep 2:  TTAGGC; Tfo rep 3: TGACCA
identifiers.namespace: GEO
identifiers.id: GSM3009673