title: Metagenomic sequences from gut content of Eisenia fetida
design: The paired-end sequencing library was prepared using illumina TruSeq DNA LT Library Preparation Kit. Library preparation was started with gDNA fragmentation of 1 g DNA, followed by paired-end adapter ligation. The ligated product will be purified using 1X Ampure beads. The purified ligated product was subjected to size-selection at ~650-1000 bp. The size-selected product was PCR amplified as per manufacture s protocol. After obtaining the Qubit concentration for the library and the mean peak size from Bioanalyser profile, Library was loaded onto MiSeq for cluster generation and paired-end sequencing.  High quality reads was assembled using CLC workbench with default parameters (mismatch cost 2, insertion cost 3, deletion cost 3, length fraction 0.5, similarity fraction 0.8 etc.).
identifiers.namespace: SUB3878884
identifiers.id: RC_R1.fastq.gz