title: GSM4671393: ChIPseq sample 1: PAO1 RepC ChIPseq background control; Pseudomonas aeruginosa PAO1; ChIP-Seq
library_construction_protocol: Cultures were treated with a final concentration of 1% formaldehyde before lysis with a sonicator. For each sample, 20 uL of antibodies and 20 uL of ChIP-Grade Protein A/G Magnetic beads were added to 800 uL of sample and incubated overnight at 4°C on a rotisserie Libraries were prepared from purified immunoprecipitated DNA using the TruSeq ChIP Sample Preparation Kit as per manufacturer's instruction. The sequencing was done using the MiSeq Reagent Kit V3 175 cycles.
identifiers.namespace: GEO
identifiers.id: GSM4671393