title: GSM4125394: CK99 - DMPA; Homo sapiens; RNA-Seq
library_construction_protocol: Genomic DNA was extracted from the CVL supernatants using a modified DNA isolation method as described in Stearns et al. (Stearns et al., 2015). CVL supernatants were inverted to mix and transferred to screw cap tubes containing 2.8mm ceramic beads, 0.1mm glass beads, GES, and sodium phosphate buffer. Samples were bead beat and centrifuged as described in Stearns et al., 2015 and the resulting supernatant was further processed using the robotic MagMAX Express 96-Deep Well Magnetic Particle Processor (Applied Biosystems, Foster City, CA) with the Multi-Sample kit (Life Technologies #4413022, Burlington, Canada). sl1p pipeline (Whenlan and Surette, 2017, BMC Microbiome) 16S rRNA gene sequencing
identifiers.namespace: GEO
identifiers.id: GSM4125394