title: GSM2481068: IP KD2 Day8 Rep1; Homo sapiens; RIP-Seq
library_construction_protocol: Total RNA was isolated from MOLM13 Ctrl cells or METTL3 KD cells 8 days after doxycycline administration using the RNeasy midi Kit (Quiagen). Successively polyA+ RNA was purified from 300 ug of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). 500 ng of polyA+ purified RNA were used for each immunoprecipitation reaction. The m6A RNA immunoprecipitation was performed using the Magna MeRIP m6A kit (Millipore) according with the manufacturer instructions. Single end 50 bp stranded libraries were prepared using the Bioo Scientific NEXTflex Rapid Directional RNA kit following manufacturer's recommendations. Reads were sequenced using HiSeq 4000 and multiplexed reads were split based on their barcodes using Illumina Basespace.
identifiers.namespace: GEO
identifiers.id: GSM2481068