title: Illumina HiSeq 2000 sequencing; RNA-Seq of murine total lung RNA samples after AAV6.2-mediated Il13 overexpression
design: RNA-Seq of murine total lung RNA samples after AAV6.2-mediated Il13 overexpression
library_construction_protocol: 0.9x10^11 vg of AAV6.2 vectors containing the murine Il13 cDNA were administered intratracheally to Balb/c mice (9-12 wk). As a control, AAV6.2 vectors containing non-coding “stuffer DNA” were used. To assess vector-mediated effects following AAV6.2 administration, expression in AAV-stuffer treated animals was further compared to mice treated with PBS. cDNA libraries were prepared using the TruSeqTM RNA Sample Preparation Kit (# RS-122-2002, Illumina), low throughput protocol according to Illumina’s instructions
identifiers.namespace: BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG, DRUG METABOLISM AND PHARMACOKINETICS
identifiers.id: E-MTAB-2598:203_75_mIL13
identifiers.namespace: Boehringer Ingelheim Pharma GmbH & Co. KG, Drug Metabolism and Pharmacokinetics
identifiers.id: E-MTAB-2598:203_75_mIL13