title: GSM4122831: lomA mutant_RNA m2011-1; Leptospira interrogans serovar Manilae; RNA-Seq
library_construction_protocol: Cells were collected by centrifugation and pelleted cells were resuspended in TRIzol. RNA was then purified according to the manufacturer's instructions (Invitrogen, Saint Aubin, Ile de France, France). DNA was degraded by using a Turbo DNA-free kit according to the manufacturer's instructions (Invitrogen). From 0.5 μg of total RNA, we depleted ribosomal RNAs using the Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina. On these rRNA-depleted RNAs, the sequencing libraries were constructed using the TruSeq Stranded mRNA Sample preparation kit following the manufacturer's instructions (Illumina). The directional libraries were controlled on Bioanalyzer DNA1000 Chips (Agilent Technologies) and concentrations measured with the Qubit® dsDNA HS Assay Kit (ThermoFisher).  Sequences of 65 bases were generated on the Illumina Hiseq 2500 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM4122831