title: Illumina HiSeq 2500 sequencing; The effect of Dis3 knockdown on miRNA expression in Drosophila wing imaginal discs.
design: The effect of Dis3 knockdown on miRNA expression in Drosophila wing imaginal discs.
library_construction_protocol: 60 wing imaginal discs per sample were dissected from wandering 3rd instar larvae. Nubbin-Gal4 homozygous females were crossed to homozygous UAS-Dis3RNAi males at 25 degrees C.All offspring were of knockdown (nubbin-Gal4/UAS-Dis3RNAi) genotype. RNA was extracted from each sample of 60 wing discs using a miRNeasy kit. RNA was size selected (18-22nt) then libraries were constructed using illumina TruSeq Small RNA sample preparation protocol.
identifiers.namespace: BRIGHTON AND SUSSEX MEDICAL SCHOOL
identifiers.id: E-MTAB-3569:Nubbin-GAL4 1
identifiers.namespace: Brighton and Sussex Medical School
identifiers.id: E-MTAB-3569:Nubbin-GAL4 1