title: GSM1401488: Pabyssi_RNAseq; Pyrococcus abyssi; RNA-Seq
library_construction_protocol: Total RNAs were extracted using the TRIzol method (Invitrogen). A pool of equal amounts of each sample was checked for integrity by 2100 Bioanalyzer (Agilent Technologies Inc.) and treated to enrich in primary transcripts using a 5'-phosphate-dependent exonuclease (Terminator, Epicenter). RNA samples were subsequently purified and concentrated using the RNA Clean-Up and Concentration Kit (Norgen Biotek Corp). The strand-specific RNA-seq template library was prepared starting from a pool of total samples (50 ng) following the directional mRNA-seq library preparation protocol provided by Illumina Inc.
identifiers.namespace: GEO
identifiers.id: GSM1401488