title: GSM4332256: Leaves, GmNRPD1-RNAi-NT-mRNA-r1; Glycine max; RNA-Seq
library_construction_protocol: Mature leaf tissues were quickly frozen in liquid nitrogen and ground to a fine powder using a mortar and pestle. Total RNA was isolated from the powder using TRI Reagent from the Molecular Research Center (MRC, TR118) following the manufacturer's protocol. Poly(A) mRNA was isolated from five µg of mature leaf total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, E7490), and the purified mRNA was used to construct the mRNA-seq library using the NEBNext® mRNA Library Prep Reagent Set for Illumina® (NEB, E6100L). The libraries were sequenced on an Illumina Illumina HiSeq 2500 at the UC Riverside IIGB Genomics Core.
identifiers.namespace: GEO
identifiers.id: GSM4332256