title: GSM2564725: MC2_Input; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde, lysed, and sonicated. Sonicated DNA fragments that bound to H3K4me1 or H3K27ac were immunoprecipitated with anti-H3K4me1 antibody or anti-H3K27ac antibody, respectively. The immunoprecipitated DNA was repaired, ligated to adapters, and size selected with the Ion Plus Fragment Library Kit (#4471252, Thermo Fisher Scientific, Waltham, MA) according to the Ion ChIP-Seq Library Preparation protocol. Libraries were analyzed for size and quantity using the High Sensitivity Bioanalyzer Kit (#5067-4626, Agilent Technologies, Santa Clara, CA). The ChIP library was sequenced on an Ion Torrent Proton Semiconductor Sequencer.
identifiers.namespace: GEO
identifiers.id: GSM2564725