title: GSM4495109: BAT_IgG1_5 [bat11_S29_L007]; Mus musculus; RNA-Seq
library_construction_protocol: Gastrocnemius muscle (GN), white and brown adipose were flash frozen prior to RNA isolation.  RNA was prepared using the Qiagen RNAeasy kit with on column DNAse treatment.  Brown adipose RNA was prepared for sequencing using the UMI second strand synthesis module for QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and sequenced on an Illumina NovaSeq 6000 (100bp single-end reads and 5-10 million reads were generated for each sample). White adipose and gastrocnemius muscle RNA were prepared for sequencing using the TailorMix Directional RNA Sample Preparation Kit (SeqMatic) and sequenced on an Illumina NovaSeq 6000 (100bp single-end reads and 30-50 million reads were generated for each sample).
identifiers.namespace: GEO
identifiers.id: GSM4495109