title: GSM3014578: KRT8-traced normal bladder cells [CTB030]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the MagMAX-96 RNA isolation kit (Ambion). Quality control was performed with Agilent Bioanalyser 2100. We use poly-A pull-down to enrich mRNAs from total RNA samples (5ng-200ng per sample, RIN>8 required) and proceed on library preparation by using Illumina TruSeq RNA prep kit. Libraries are then sequenced using Illumina HiSeq2500 at Columbia Genome Center. We multiplex samples in each lane, which yields targeted number of single-end/paired-end 100bp reads for each sample, as a fraction of 180 million reads for the whole lane.
identifiers.namespace: GEO
identifiers.id: GSM3014578