title: RNA-Seq of MBR
design: The enriched mRNA was fragmented into short fragments (approximately 202 bp) randomly using metal ions. Then the first strand cDNA was synthesized using the random hexamer-primer with the mRNA fragment as the template. After synthesizing the second strand cDNA using DNA polymerase I and RNase H, double-stranded cDNA was further end repaired, A-tailed, and indexed adapters ligated. The final cDNA library was constructed using the mRNA-Seq library construction kit
identifiers.namespace: SUB7318315
identifiers.id: C3