title: GSM3384136: Patient3_WT_noTreat_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Primary CLL cells were harvested after purity check (>95% CD5 ve+/CD19 ve+) and total RNA was extracted using the standard protocol from GenElute Mammalian Total RNA Miniprep Kit (Sigma/RTN70). NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB #7420) was used with 100ng of total RNA for the construction of sequencing libraries. mRNA was isolated and purified by using NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB #7490) before cDNA synthesis.
identifiers.namespace: GEO
identifiers.id: GSM3384136