title: GSM4495075: MUT_NT_2h_2; Homo sapiens; RNA-Seq
library_construction_protocol: Following in vitro cytokine stimulation, cells were harvested by lysing the pellet in Qiagen Buffer RLT with 10μL β-mercaptoethanol/1 mL RLT. RNA was then immediately extracted from cell lysates using Qiagen RNeasy Columns according to the manufacturer's protocol. Note that the optional on-column DNase digestion was performed. RNA integrity was subsequently analyzed using the Bioanalyzer 2100 Eukaryote Total RNA Nano chip. All samples had RIN = 10. Samples were normalized to 1ug total RNA and the mRNA fraction was isolated by polyA selection. Libraries were prepared using the TruSeq Stranded mRNA Kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM4495075