title: GSM4062097: SacCer_minus_minus_3; Saccharomyces cerevisiae; OTHER
library_construction_protocol: For HEK 293T cells, total RNA is extracted using phenol:chloroform method. For yeast, total RNA is extracted using the hot-acid-phenol method. Ribosome-protected footprints were purified from monosome fractions by size selection and gel extraction. Following 3' dephosphorylation these fragments were subjected to adaptor ligation, RT, circularization and PCR amplification.
identifiers.namespace: GEO
identifiers.id: GSM4062097