title: GSM1941737: IM rep2; Bos taurus; miRNA-Seq
library_construction_protocol: RNA-seq: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. miRNA-seq: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for miRNA purification. miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by Gel electrophoresis. RNA-seq: Ion  Proton Sequencing Protocol miRNA-seq: Hiseq Sequencing Protocol
identifiers.namespace: GEO
identifiers.id: GSM1941737