title: GSM3384413: WTstroma_CLL_C; Mus musculus; RNA-Seq
library_construction_protocol: Purified BMSCs were harvested after incubating with Dynabeads® CD19 pan B to deplete CLL cells. Total RNA was extracted using the standard protocol from GenElute Mammalian Total RNA Miniprep Kit (Sigma#RTN70-1KT). NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB #7420) was used with 100ng-200ng of total RNA for the construction of sequencing libraries. mRNA was isolated and purified by using NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB #7490) before cDNA synthesis.
identifiers.namespace: GEO
identifiers.id: GSM3384413