title: GSM3538079: KW6c: Sample3-post-ICH; Sus scrofa; RNA-Seq
library_construction_protocol: For each sample, approximately 10 ml of swine peripheral blood was collected and immediately placed into EDTA containing blood collection tubes, inverted multiple times, and processed within one hour. Briefly, Lymphoprep™ (Stemcell Technologies, Cambridge, MA) density gradient medium and SepMate™ (Stemcell Technologies) tubes were used according to the manufacturer's instructions to isolate PBMCs. The PBMCs were directly lysed with Lysis Buffer from the mirVana miRNA isolation kit (Thermo Fisher, Waltham, MA) to stabilize RNA until the time of RNA extraction as described in the total RNA extraction protocol from the kit.). To isolate the polyA RNA, NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used with a total of 1 µg of good quality total RNA as input. The NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs) was used for library preparation.
identifiers.namespace: GEO
identifiers.id: GSM3538079