title: GSM1616763: GSC-222_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from GSCs using TRIzol (Life Technologies). The RNA-seq libraries were prepared using a paired-end RNA Sequencing Sample Prep Kit (Illumina). 2 μg of total RNA was used as the starting material, and the polyadenylated RNAs were selected using Sera-Mag Magnetic Oligo(dT) Beads (Illumina). First-strand cDNA synthesis was performed using SuperScript II (Life Technologies), and second-strand cDNA synthesis was performed using DNA Pol I in the supplied GEX second-strand reaction buffer (Illumina). Paired-end adaptors were ligated to the cDNA fragments. PCR (eight cycles) was performed with Phusion High-Fidelity DNA Polymerase (Finnzymes Oy, Espoo). The PCR products were cleaned up with Agencourt AMPure XP magnetic beads (Beckman Coulter).
identifiers.namespace: GEO
identifiers.id: GSM1616763