title: GSM3685045: SKBR3_Cy0_S83; Homo sapiens; OTHER
library_construction_protocol: Cells were trypsinized, diluted to 1000cells/ml and handpicked as single cells in 10x Lysis buffer Single cells isolated into 10x Lysis Buffer with Rnase inhibitor were processed for mRNA transcription for first strand synthesis and cDNA amplification following SMART-Seq2 protocol. Samples were then treated ionically to fragment gDNA and cDNA to 400-700bp fragments. Later gDNA was amplified using SeqXE kit from Sigma Aldirch using manufacturer instructions. Libraries were made by end repair, Adenylation, Adapter ligation and PCR steps.
identifiers.namespace: GEO
identifiers.id: GSM3685045