title: GSM3929163: Wheat.shoot.Hi-C; Triticum aestivum; Hi-C
library_construction_protocol: The experiment was carried out according to the protocol published by Liu et al., 2017 using DpnII enzyme (New England Biolabs) with minor modifications concerning library preparation for which we used NebNext UltraII DNA library preparation kit (New England Biolabs). For library amplification, nine PCR cycles were performed and Hi-C libraries were purified with SPRI magnetic beads (Beckman Coulter) and eluted in 20 μL of nuclease-free water. The quality of the libraries was assessed with Agilent 2100 Bioanalyzer (Agilent)
identifiers.namespace: GEO
identifiers.id: GSM3929163