title: S20_ITS_14
design: Sequencing at Molecular and Cellular Imaging Center (MCIC) at Ohio State University following the protocols of Caporaso et al. (2012).  The fungal ITS1 region was amplified using primers ITS1F (5- CTT GGT CAT TTA GAG GAA GTA A  3) and ITS2 (5  GCT GCG TTC TTC ATC ATC GAT GC  3). PCR amplicons were quantified by PicoGreen (Invitrogen, Carlsbad, CA) using a plate reader, and amplicons were pooled in equal concentrations into a single tube. Five replicate PCRs were pooled and purified using Agencourt AMPure XP magnetic beads (Agencourt Bioscience Corporation, MA, USA). The pooled samples were sequenced on the Illumina MiSeq platform (Illumina, San Diego, CA) according to the sequencing procedures described in Caporaso et al. (Caporaso et al., 2012).
identifiers.namespace: SUB7608150
identifiers.id: S20.ITS