title: GSM4278005: Sham 1 SA P4; Mus musculus; RNA-Seq
library_construction_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instruction. RNA-seq was performed by Novogene (California, U.S.A.). From total RNA, rRNA was removed using Ribo-Zero rRNA Removal kit (Illumina, #MRZH11124). Then, rRNA-depleted total RNA was subjected to the library preparation using  NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, #E7770). The insert size of 250~300bp were sequenced in a directional manner using The HiSeq 4000 System Illumina (pair-end, 2 × 150 bp).
identifiers.namespace: GEO
identifiers.id: GSM4278005