title: GSM4618787: Pancreatic islet, HI-070719-DMSO; Homo sapiens; RNA-Seq
library_construction_protocol: After 24 hours of Brefeldin A treatment, islets were collected, washed once with PBS, lysed in Trizol. Total RNA was isolated using the miRNAeasy kit (Qiagen). Total RNA sequencing utilized 100 ng of RNA as input. Libraries for total RNA sequencing were prepared using KAPA mRNA Hyperprep Kit (Roche Sequencing & Life Science). Sequencing was performed using Illumina NovaSeq 6000 (2x100 bp paired-end reads), generating 30 million reads per library
identifiers.namespace: GEO
identifiers.id: GSM4618787