title: GSM3538062: HuCCA-1_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: RNA were extracted using Trizol, purified by Ambion RNA isolation kit (Invitrogen) and treat with PureLink DNase set (Invitrogen). RNA samples were quantified by measuring absorbance at 260nm with Qubit RNA HS assay kit. The integrity of RNA was analyzed by running on agarose gel electrophoresis and measuring RIN value by Agilent 2100 Bioanalyzer (Agilent Technologies). All CCA sample show high integrity of RNA with intact 28sRNA, intact 16sRNa and RNA integrity number(RIN)>8. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM3538062