title: GSM4121500: ChIP-seq: T_Cell_H3K79me2_WT_Memory_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Flow cytometrically purified cells were cross-linked, and chromatin lysates were prepared. Histone-DNA complexes were isolated with antibodies. Library preparation was done using KAPA LTP Library preparation kit using the manufacturer's protocol with slight modifications. Briefly, after end-repair and A-tailing adaptor were ligated followed by SPRI clean-up. Libraries were amplified by PCR and fragments between 250-450 bp were selected using AMPure XP beads (Beckman Coultier). The libraries were analyzed for size and quantity of DNAs on a 2100 Bioanalyzer using a High Sensitivity DNA Kit (Agilent), diluted and pooled in multiplex sequencing pools. The libraries were sequenced as 65 base single reads on a HiSeq2500 (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM4121500