title: GSM4061950: hRBO-D120; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated from each sample using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturer's protocol. DNA was quantified using NanoDrop 2000 and Qubit 2.0 Fluorometer. 1µg genomic DNA was used to construct WGBS library. Bisulfite conversion of genomic DNA was performed using the EZ DNA Methylation-Gold Kit (Zymo Research) according to the manufacturer's recommendation. Bisulfite-converted DNA was subjected to process as described, and then sequencing (PE125/PE150) on an Illumina HiSeq platform.
identifiers.namespace: GEO
identifiers.id: GSM4061950