title: GSM4061782: 785: control 5 (saline at pnd 7); Rattus norvegicus; RNA-Seq
library_construction_protocol: P14 rats (seven days post KA-ELS) were decapitated without anesthesia and the brain rapidly removed.  Dorsal hippocampal slices were prepared by slicing the brain into parasagittal slices (400 μm) on a Leica VT 1200 microtome in ice cold solution containing (in mM) 206 Sucrose, 2.8 KCl, 1.25 NaH2PO4, 26 NaHCO3, 10 Glucose, 10 MgSO4, 2 NaAscorbate, 0.4 CaCl2, and 2.5 N-acetyl L-cysteine.  Scalpel cuts were made to remove CA3 while retaining the CA1 region of the hippocampus with the overlying cortex and dentate gyrus intact during recovery.  All slices from every animal were uniformly recovered for 60 min at room temperature in a submersion chamber in standard artificial Cerebral Spinal Fluid (aCSF), containing (in mM) 124 NaCl, 26 NaHCO3, 1.2 NaH2PO4, 10 D-glucose, 3 KCl, 2 NaAscorbate, 1 MgSO4, 2 CaCl2, and 2.5 N-acetyl L-cysteine) prior to all experiments in order to correlate to previous studies.  All solutions were oxygenated with 95% O2 - 5% CO2.  After recovery, the CA1 region of the hippocampus was further isolated from the slices for RNA extraction. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM4061782