title: GSM3537309: SB2: Sample 5_SA187_Rep2_MMS; Enterobacter sp. SA187; RNA-Seq
library_construction_protocol: Total RNA was extracted from 17-day-old seedlings flash frozen in liquid nitrogen, using NucleoSpin® RNA Plant extraction kit (Macherey-Nagel) following manufacturer's instructions,  was used with 1 ug of total RNA for the construction of sequencing libraries. Total RNA from bacteria pure culture was extracted by using RiboPure™ RNA Purification Kit (Invitrogen), following manufacturesr's intructions with some minor modifications Total RNA from bacteria and dual (plant + bacteria) samples was treated with Ribo-Zero Magnetic Gold Kit to remove ribosomal rRNA (rRNA). For rRNA depletion of bacteria samples (B and SB), rRNA Removal solution from Ribo-Zero Gold kit (Epidemiology) was used. For rRNa depletion of dual samples (B and BS), rRNA Removal solution from Ribo-Zero Magnetic kit (Plant Leaf) and rRNA Removal Solution from Ribo-Zero Gold kit (Epidemiology) were mixed in a ratio 1:1. An amount of 1 ug of total RNA was used for the contruction of RNA-Seq libraries. Pair-end sequencing of the 6 dual (plant + bacteria) RNA-Seq libraries was performed by using Illumina Genome Analyzer IIx (GAIIx), while Pair-end sequencing of the 6 bacteria RNA-Seq libraries was performed by using Illumina HiSeq 2500. Read length was 101 bp
identifiers.namespace: GEO
identifiers.id: GSM3537309