title: T2C for 30 minutes (HindIII) replicate 2
design: T2C was performed in duplicates as described ( Kolovos et al., 2014). In brief, ~10 million HUVEC nuclei were isolated at 0, 30 min post-stimulation, following cross-linking in 1% paraformaldehyde (10 min, 20°C; Electron Microscopy Science), chromatin was cut using ApoI or HindIII (New England Biolabs), and ligated in dilution under conditions that allowed most nuclei to remain intact.
identifiers.namespace: Erasmus MC
identifiers.id: T2C for 30 minutes (HindIII) replicate 2