title: GSM1942692: sAB1; Canis lupus familiaris; RNA-Seq
library_construction_protocol: Total RNA was isolated from 1x106 cells using RNeasy kit (Qiagen).  RNA integrity was analyzed on an Agilent Technologies 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit (Agilent Technologies) and only samples with an RNA integrity number (RIN) above 9.5 was used for further analysis. cDNA and libraries were prepared using 1 µg total RNA and the TruSeq mRNA sample preparation kit according to the manufacturer’s instructions (Illumina).  Library quality was analysed on an Agilent Technologies 2100 Bioanalyzer using the Agilent DNA 1000 kit (Agilent Technologies).  Library samples were quantified using the Qubit dsDNA BR assay kit (Invitrogen) and diluted to 10 nM before single read sequencing on an Illumina HiSeq 2000 sequencing platform, performed at the National High-throughput DNA Sequencing Centre, University of Copenhagen.
identifiers.namespace: GEO
identifiers.id: GSM1942692