title: GSM4493867: Sample_8 (KDEE); Mus musculus; RNA-Seq
library_construction_protocol: Hippocampi were extracted and then rapidly homogenized in Trizol. Total RNA was precipitated using isopropanol following the manufacturer's protocol and treated with DNaseI. RNA quality was checked using Nanodrop and a Qubit 4 fluorimeter (Invitrogen). 150bp paired-end mRNA libraries were prepared using the TruSeqv2 (Illumina, #RS-122-2001) LS protocol. Briefly, poly-A mRNA was pulled down using poly-T magnetic beads, fragmented, and primed with random hexamers before first-strand synthesis. Following second-strand synthesis, blunt end repair was performed with a 3' to 5' exonuclease, and 3' ends adenylated. Adaptors were then ligated to the cDNA. All 24 library samples were quality checked on a Agilent 2100 bioanalyser and assayed on a Qubit 4 fluorimeter.
identifiers.namespace: GEO
identifiers.id: GSM4493867