title: GSM2829910: col_293457; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Pollen was collected in 1.5mL eppendorf tubes by vortexing open flowers in pollen extraction buffer (PEB, 10 mM CaCl2, 2 mM MES, 1 mM KCl, 1% H3BO3, 10% Sucrose, pH 7.5) for 3 min (31), followed by filtration through a 30um mesh (Partec/Sysmex) and centrifugation at 5,000g for 1 min. Pollen was suspended in 50ul of PEB, immediately frozen in liquid nitrogen and stored at -80ºC until further use. Libraries for RNA sequencing from Col-0 and Ler-0 pollen were prepared using rRNA-depleted total RNA samples and the ScriptSeq-v2 RNA-Seq Library Prep kit (Epicenter/Illumina), following manufacturer instructions.
identifiers.namespace: GEO
identifiers.id: GSM2829910