title: GSM3986327: Sample 4 with SDC-1 siRNA silencing and 2ng/ml TGF-β stimulation [SF4_S79]; Homo sapiens; RNA-Seq
library_construction_protocol: Invaded 3D spheroids were lysed using TRIzol reagent (Ambion® Thermo Fisher Scientific, Waltham, USA) and subsequent RNA extraction was performed using Ethanol precipitation method. cDNA library was constructed using well established Smart-seq2 protocol (Picelli et al. 2013, Picelli et al. 2014), tagmentation step was accomplished using Nextera XT kit (Illumina) and sequencing was done in Nextseq 550® at the bioinformatics expression analysis facility at Karolinska University hospital, Sweden.
identifiers.namespace: GEO
identifiers.id: GSM3986327