title: GSM4333107: 1009902203; Toxoplasma gondii RH; RNA-Seq
library_construction_protocol: All cells were sorted directly into lysis buffer containing (per every 8 ml) 5.888 ml water, 160 Âµl recombinant RNase inhibitor (Takara Clonetech), 1.6 ml of 10 mM dNTP (Thermo Fisher), 160 Âµl of 100 M oligo-dT (iDT), 1:600,000 diluted ERCC spike-in RNA molecules (Thermo Fisher), and 32 Âµl of 10 % Triton X-100. RNA was converted to cDNA using the SmartSeq2 protocol, with 19 rounds of pre-amplification PCR.  All samples were quantified using EvaGreen and diluted in EB buffer to obtain a final concentration of 0.4 - 0.8 ng/Âµl per sample. Library preparation continued using in-house Tn5 for tagmentation. For single cell libraries, custom barcoded indices were used for each cell, and for bulk libraries, Nextera XT indices were used for each sample. Smart-seq2 single cell RNA-sequencing.
identifiers.namespace: GEO
identifiers.id: GSM4333107