title: GSM1941473: Brg1 knockdown pol II ChIP-exo replicate 1; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP-exo using an antibody anti-RNA polymerase II: The immunoprecipitation step was performed using antibodies against Pol II (sc-899, Santa Cruz Biotechnology) attached to magnetic beads, followed by DNA polishing, A-tailing, Illumina adaptor ligation (ExA2), and lambda and recJ exonuclease digestion on the beads. After elution, a primer was annealed to EXA2 and extended with phi29 DNA polymerase, then A-tailed. A second Illumina adaptor was then ligated, and the products PCR-amplified and gel-purified.
identifiers.namespace: GEO
identifiers.id: GSM1941473