title: GSM1399614: wt_yesleu_di; Escherichia coli str. K-12 substr. MG1655; ncRNA-Seq
library_construction_protocol: Rapid filtration followed by flash freezing of intact cells, cell lysis by glass bead beating, Micrococcal nuclease digestion of polysomes, purification of monosomes/disomes by sucrose-gradient centrifugation. Total mRNA was purified from the cell lysate by phenol-chloroform method, rRNA-subtracted (MicrobeExpress, Ambion) and heat fragmented for use in  sequencing library preparation. A modified small RNA protocol (Subramaniam et al., in preparation) was used for both the construction of both ribosome-protected and total mRNA sequencing libraries.
identifiers.namespace: GEO
identifiers.id: GSM1399614