title: GSM4121364: DND41_GSI_Res_sustained_scRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Prior to single–cell transcriptomic profiling, cells were washed with 1 x PBS and stained with DAPI and live cells were sorted on BD FACS Aria II using 100 μm nozzle. Cells were washed twice with RPMI, counted and single-cell RNA-seq was performed using 10X Genomics Single Cell 3' Library and Gel Bead Kit v2 following the manufacturer's instruction. Briefly, Cells were loaded onto independent channels of a Chromium Controller (10X Genomics) for targeted recovery of 3,000 cells/condition. Complementary DNA was synthesized and amplified with PCR for 13 ycles. Amplified cDNA was assessed for QC and quantified on Agilent TapeStation using High sensitivity D5000 chip and subsequently used for library construction. Libraries were quantified using KAPA Library Quantification Kits for Illumina platform and pair-end sequenced on NextSeq 550 using 150 cycles High Output kit.
identifiers.namespace: GEO
identifiers.id: GSM4121364