title: Fetal heart 3: H3K4me3 human cardiac myocyte nuclei
design: Isolated cardiac myocyte nuclei were fixed with paraformaldehyde, lysed and sonicated using a Bioruptor (Diagenode) with 30 cycles (30 s on/ 30 s off; high energy). The isolated chromatin was used for the ChIP experiment. The immunoprecipitation was performed with 4  g of anti-H3K4me3 antibody (Diagenode, pAb-003-050) overnight. Reversal of cross-linking and DNA purification were performed using the ChIP-IT High Sensitivity Kit (Active Motif) following the manufacturer's manual. The eluted DNA was concentrated by evaporation at room temperature using a vacuum concentrator (Eppendorf) to 55.5  l. Libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) according to the manufacturer's manual omitting the size selection.
identifiers.namespace: SUB3380142
identifiers.id: Fe3_H3K4me3