title: GSM4672081: S2R+ M3(10%FEx) #3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 50 million cells were collected from a 10mL culture (5 million cells/mL), in triplicates. The cells were pelleted and total RNA was extracted using Trizol according to manufacturer's protocol. Total RNA were subsequently cleaned using Qiagen RNAeasy kit according to manufacturer's protocol. First, the integrity of the RNA was checked with Agilent Tapestation, samples with RIN >7 were processed using the following steps. From each sample, 1ug of total RNA was used for library preparation following the Illumina TruSeq Stranded mRNA library preparation kit protocol. The cleaned adapter ligated libraries were pooled and loaded on the NextSeq 500 with a 75 cycle sequencing module to generate paired-end reads.
identifiers.namespace: GEO
identifiers.id: GSM4672081