title: Ion Torrent PGM sequencing of SAMD00133975
library_construction_protocol: All the protocols were followed as per manufacturer's instructions. Total RNA was prepared from the 100 embryos using Quick-RNA Microprep Kit (Zymo research, USA), then rRNAs were depleted from total RNA using Low Input RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, USA). Quality control of purified RNA was then performed using the Agilent RNA 6000 Pico Kit with the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, USA). An ERCC RNA Spike-In Mix control (Thermo Fisher Scientific, USA) was then added to each sample proportionate to the quantity of total RNA. Preparation of a whole-transcriptome cDNA library from purified RNA was then performed using an Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, USA). Quality control of the cDNA libraries was then performed using the Agilent DNA 1000 Kit with the Agilent 2100 Bioanalyzer instrument (Agilent Technologies, USA).
identifiers.namespace: KYOTO_SC
identifiers.id: DRX137607