title: GSM2905081: D16-3; Capra hircus; RNA-Seq
library_construction_protocol: total  RNA was extracted using Trizol reagent. Ion Total RNA-seq Kit v2 (Life Technologies, USA) was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard protocols of Total RNA-seq Kit v2 (Life Technologies, USA). Briefly, poly(A)-containing mRNA was purified by Dynabeads (Life technologies, USA) and subsequently fractionated into short fragments using RNase III and an Ion adaptor. The short RNA fragments was ligated with a 3' adaptor and a 5' adaptor.  Emulsion PCR was performed using mRNA cDNA library as the template. Then, the short mRNA fragments were reverse-transcribed and amplified to synthetize double-stranded cDNA.
identifiers.namespace: GEO
identifiers.id: GSM2905081