title: Dasypyrum villosum Chromosome 6VS
design: Aqueous suspensions of chromosome 6VS of H. villosa were prepared from synchronized meristem root tip cells. DNA of the sorted chromosome arms was purified and then amplified by multiple displacement amplification (MDA) . Two micrograms of amplified DNA were used to prepare sequencing library using TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, USA). The library was sequenced in one run on Illumina MiSeq System (1000 bp insert, 2 x 300 bp) yielding 14 Gb sequence data (~44x coverage of 6VS). The second type of 6VS DNA sample was not amplified and DNA from 100,000 copies of 6VS telosome was purified and used to prepare sequencing library using Nextera DNA Library Prep Kit (Illumina). The library was sequenced in one run on Illumina MiSeq System (500 bp insert, 2 x 300 bp) yielding 10.2 Gb sequence data (~32x coverage of 6VS).
identifiers.namespace: SUB6566206
identifiers.id: X123456