title: GSM4332485: at-endosperm-HiC-replicate1_col_wt_x_col_wt; Arabidopsis thaliana; Hi-C
library_construction_protocol: To examine the tissue-specific spatial organization of chromatin, we adopted the previously published genome-wide high-resolution in situ Hi-C method  (Flyamer et al. 2017). The major step involved in the preparation of mHi-C library is HCHO-crosslinking, permeabilization followed by restriction enzyme digestion proximity-dependent ligation events between cross-linked restriction-digested chromatin fragments. Sequencing libraries were constructed using Ovation Rapid DR Multiplex System 1-8 (NuGEN) with 300-800 bp size selection according to the manufacturer's instructions. Samples were sequenced at the National Genomic Infrastructure (NGI) from SciLife Laboratory (Uppsala, Sweden) on an Illumina HiSeq2500 in paired-end 125bp read length.
identifiers.namespace: GEO
identifiers.id: GSM4332485