title: GSM1399018: TKO_H3K27me3_ChIPseq_1; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitation (ChIP) was performed according to the manufacture's protocol (ChIP kit, Millipore) with the following modifications: 107 cells were used per sample. After cell lysis, extracts were subjected to sonication with a 60 Sonic Dismembrator (Fisher Scientific) at 4°C for 20 cycles, cycling ON for 20 seconds and OFF for 1 minute at 4.5 power leading to a DNA average size of 200 bp. Immunoprecipitations were performed overnight at 4°C using 5 μg of antibody. Imunoprecipitated chromatin was extracted using QIAquick PCR Purification Kit (QIAGEN). 10 ng of input and ChIP material was processed using the Illumina kit (IP-102-1001)
identifiers.namespace: GEO
identifiers.id: GSM1399018