title: GSM4333319: TSG_WNT_treatment_rep2; Homo sapiens; OTHER
library_construction_protocol: Phenol Extraction and Isopropanol Precipitaiton Libraries were prepared by PCR amplification of integrated sgRNAs from gDNA. Barcoded primers were used for high throughput analysis of organoid clones. The Illumina TruSeq Nano DNA library prep was used for subquented blunt-ended ligation of sequencing adaptors.
identifiers.namespace: GEO
identifiers.id: GSM4333319