title: GSM2481212: CD11b MΦ bleomycin day 8 rep1_run2; Mus musculus; RNA-Seq
library_construction_protocol: CD64+Mertk+ lung macrophages were sorted into SiglecF(high) and CD11b(high) subsets.  RNA was isolated from sorted cells using the RNeasy micro kit (QIAGEN). A Clontech SMARTer® Ultra™ Low Input RNA Kit for Sequencing – v4 (Mountain View, CA, USA) and modified Kapa Biosystems KAPA Hyper Prep Kit (Wilmington, MA, USA) were used. Briefly, library construction started from isolation of total RNA species taking 3ng of total RNA into the protocol, followed by SMARTer 1st-strand cDNA synthesis, full length dscDNA amplification by LD-PCR, followed by purification and validation. After that, the samples were sheared using a Covaris system, vacuum concentrated and then processed through the Kapa protocol. The Kapa protocol consisted of end repair and A-tailing, adaptor ligation, amplification, and bead templating.  Libraries were sequenced as barcoded-pooled samples on a P1 Ion Proton chip.
identifiers.namespace: GEO
identifiers.id: GSM2481212