title: GSM3005163: oocyte_H3K27me3_rep1; Danio rerio; ChIP-Seq
library_construction_protocol: ChIP DNA is extracted as following: chromatins were elutted from bead-antibody complex with 200 ul ChIP elution buffer(1% SDS, 100nm NaHCO3), and then reverse crosslinked at 60 degree, 1000 rpm at thermal shaker for 6 hours. then proteinase (qiagen)  were added to final concentration 2 ug/ml, incubated at 56 degree for 30 minutes. DNA were purified with Zymo ChIP DNA Clean & Concentrator Kits (cat. No. D5201) standard illumina library prep procedure
identifiers.namespace: GEO
identifiers.id: GSM3005163