title: GSM3537188: ATAC-Seq: ARID1A-KO2 rep3; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq library construction was performed as previously described with minor modifications (PMID: 24097267) using 60,000 cells of two independent clones of human endometrial epithelial ARID1AWT and ARID1AKO cells. Transposed DNA was purified using a QIAgen MinElute PCR Purification kit, and was subsequently amplified using NEBNext High Fidelity 2x PCR master mix for 12 cycles. PCR products were purified using Agencourt AMPure XP, and sequencing was performed by JHMI Deep Sequencing and Microarray Core using a NextSeq500 platform with double-end reads of 75 bases.
identifiers.namespace: GEO
identifiers.id: GSM3537188