title: GSM4332647: MAIT_SS_WT_1; Mus musculus; RNA-Seq
library_construction_protocol: WT and Bcl11b KO MAIT cells were sorted from naïve or salmonella infected mouse lungs. Cells were lysed and RNA was isolated using Qiagen Rneasy Plus Micro Kit (Qiagen #74034). RNA quality was determined on a bioanalyzer (Agilent RNA 6000 Nano #5067-1511) Libraries were prepared using New England Biolabs NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (cat# E7770S) according to the manufacturer's instructions. Library quality and concentration was checked on a bioanalyzer (Agilent High Sensitivity DNA Kit #5067-4626) and by Qubit (Qubit™ dsDNA HS Assay Kit #Q32854). Libraries were sequenced on a NextSeq500.
identifiers.namespace: GEO
identifiers.id: GSM4332647