title: GSM3684865: WUS-GR MOCK 2 small RNA-seq; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: The shoot apexes were flash frozen in liquid nitrogen, and total RNA were isolated using the mirVana™ miRNA Isolation Kit (lif tetechnology).Total RNA was fractionated by electrophoresis. RNA from 10 to 60 nt was sliced and recovered. The 10-60 nt small RNA were selected for library building.The purified small RNA was subjected to adapter ligation, reverse transcription, PCR amplification with NEXTflex Small RNA Sequencing Kit (Bioo Scientific), and the resulting libraries sent for deep sequencing by Illumina HiSeq2000.
identifiers.namespace: GEO
identifiers.id: GSM3684865