title: GSM1941485: Brg1 knockdown ATAC-seq replicate 1; Mus musculus; OTHER
library_construction_protocol: 50,000 cells were collected, washed with cold PBS and resuspended in 50ul of ES buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2). Permeabilized cells were resuspended in 50 ul Transposase reaction (1X Tagmentation buffer, 1.0-1.5 ul Tn5 transposase enzyme (Illumina)) and incubated for 30min at 37°C. Subsequent steps of the protocol were performed as described in PMID: 24097267. Transposed DNA was subjected to several round of amplification with Ad2 primer pairs (the reverse primer contains the index allowing subsequent multiplexing. Oligonucleotide sequences are available in PMID: 24097267). Libraries were next purified using a Qiagen MinElute kit and Ampure XP magnetic beads (1:1.6 ratio) to remove remaining non ligated adapters.
identifiers.namespace: GEO
identifiers.id: GSM1941485