title: GSM4494863: HSPCs, LT-HSC KO2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA were extracted from 10.000 to 40.000 LT-HSC, MPP3 and MPP4 purified cells from WT and Helios KO bone marrow cells using the RNeasy Plus Micro Kit (Qiagen 74034). Full length cDNAs were generated from 1000 pg of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe, Saint Germain en Laye, France) according to manufacturer's instructions with 12 cycles of cDNA amplification by Seq-Amp PCR. 600 pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation with the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification. Following purification with Agencourt AMPure XP beads (Beckman-Coulter, Villepinte, France), the size and concentration of libraries were assessed by capillary electrophoresis.
identifiers.namespace: GEO
identifiers.id: GSM4494863