title: GSM1943617: RatID_5_mRNA; Rattus norvegicus; RNA-Seq
library_construction_protocol: Tissue was collected at 3 months after TBI induction. For tissue collection rats were anesthetized with 4% isoflurane and decapitated with guillotine. Left hippocampus was rapidly isolated and tissue containing CA3 and dentate gyrus was homogenized in ice cold 1x PBS and divided into equal volumes for DNA and RNA extraction. Samples were stored at -80oC until use. 10 mg rat hippocampal tissue was used for total RNA extraction using the Trizol method, followed by DNase digestion. RNA quality was verified on the Shimadzu MultiNA capillary electrophoresis system (Shimadzu). Poly-A mRNA was isolated with Dynabead Oligo(dT) enrichment (Invitrogen). Sequencing libraries were prepared using the NEBNext® mRNA Library Prep Reagent Set for Illumina® (New England Biolabs). Libraries were quantified on MultiNA system and sequenced at a concentration of 13 pM on the Genome Analyzer IIx (Illumina Inc, CA, USA) using the TruSeq version 5 cluster kit (cBot) and SBS kit (36 cycles).
identifiers.namespace: GEO
identifiers.id: GSM1943617