title: GSM3084657: InR_EtOH_M1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Adult heads were separated from the body by mechanical tapping in the cryovial and then separated while frozen on a piece of plexiglass cooled on dry ice. Frozen heads were immediately transferred to Trizol reagent. Total RNA was extracted using the Trizol reagent protocol (Thermo Fisher) and was DNase treated with Turbo DNase and purified  (Zymo). RNA-seq libraries were generated using 2ug total RNA. The NEBNext Ultra RNA Library Prep Kit for Illumina, with the polyA purification module (New England Biolabs Inc. E7530L), was used for Illumina library generation.
identifiers.namespace: GEO
identifiers.id: GSM3084657