title: GSM3986052: RNAseq_SI_MRF_r4; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were recovered from decellularized ECM gels and MRF control gels with 20 min incubation in Cell Recovery Solution (Corning) on ice. Cells were lysed with RLT buffer and RNA was isolated with RNeasy mini kit (Qiagen) following manufacturer instructions. Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit  (Lexogen GmbH) according to manufacturer's instructions. The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the Nova Seq 6000 (Illumina) with a read length of 100 bp 3' Digital Gene Expression
identifiers.namespace: GEO
identifiers.id: GSM3986052