title: GSM4495063: E151G_E01; Danio rerio; RNA-Seq
library_construction_protocol: The muscle tissue were grounded using Microtube Pellet Pestle Rods (Violet BioScience, Taipei, Taiwan). TRI Reagent® (Sigma-Aldrich, MI, USA) was mixed with sample and homogenize using a homogenizer. 1/5 of chloroform was added and transfer the supernatant containing RNA to a new Eppendorf. After extracted the total RNA from muscle tissue, total RNA used NucleoSpin® RNA kit (MACHEREY-NAGEL, Nordrhein-Westfalen, Germany) for the cleanup. RNA was isolated using a NucleoSpin® RNA column (MACHEREY-NAGEL, Nordrhein-Westfalen, Germany) per manufacturer's protocol. Finally, the concentration were measured using a NanoDrop ND-1000 (Thermo Fisher Scientific, MA, USA), which were then stored in a –80°C freezer. Complementary DNA (cDNA) was synthesized using iScript™ cDNA synthesis kit (BioRad, CA, USA). All RNA sample preparation procedures were carried out according to the Illumina's official protocol. Agilent's SureSelect Strand-Specific RNA Library Preparation Kit was used for library construction followed by AMPure XP beads (Beckman Coulter, USA) size selection.
identifiers.namespace: GEO
identifiers.id: GSM4495063