title: GSM3537045: Infected_WT; Mus musculus; RNA-Seq
library_construction_protocol: WT:Tbx21-/- mixed bone marrow chimeras were generated by combining CD45.1+ WT bone marrow cells with CD45.2+ Tbx21-/- bone marrow cells in a 1:1 ratio and transplanting mixture of cells into a lethally irradiated CD45.1+ WT recipient. 8 weeks later, some mice were infected with 200 tachyzoites of the Prugniaud strain of T. gondii. Thirty-five days post infection, liver lymphocytes were isolated. CD3– CD19– NK1.1+ NKp46+ that were either CD45.1+ or CD45.2+ were separately FACS sorted Droplet-based 3′ end massively parallel single-cell RNA sequencing (scRNAseq) was performed by encapsulating sorted live CD45+ tumor infiltrating cells into droplets and libraries were prepared using Chromium Single Cell 3′ Reagent Kits v1 according to manufacturer's protocol (10x Genomics).  The generated scRNAseq libraries were sequenced using an Illumina HiSeq2500. 14x98nt read lengths were used.
identifiers.namespace: GEO
identifiers.id: GSM3537045