title: GSM4182274: Z919OT; Mus musculus; RNA-Seq
library_construction_protocol: cell pellets were flash frozen. Cells were lysed using the QiaShredder kit. RNA was isolated with the Qiagen Rneasy Micro Kit including DNase digestion. After isolation, RNA quality was checked on a Fragment analyzer and concentrqation was meausred with the Qubit. RNA was diluted to a concentration of 20 ng/ul and frozen until library preparation. The stranded TruSeq RNA Sample Prep Kit v2 (Illumina, Inc, CA, USA) was used in the succeeding steps. Briefly, mRNA was enriched from total RNA samples (100-1000 ng) using poly-A selection and then reverse-transcribed into double-stranded cDNA. The cDNA samples were fragmented, end-repaired and polyadenylated before ligation of TruSeq adapters containing the index for multiplexing Fragments containing TruSeq adapters on both ends were selectively enriched with PCR. The quality and quantity of the enriched libraries were validated using the Qubit® (1.0) Fluorometer and the Caliper GX LabChip® GX (Caliper Life Sciences, Inc.,). The libraries were normalized to 10 nM in 10 mM Tris-Cl/0.1% Tween 20, pH8.5.
identifiers.namespace: GEO
identifiers.id: GSM4182274