title: GSM4123893: Salivary gland, BxlglRNAiRasV12_2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA of dissected salivary glands was isolated with the RNAqueous-Micro Kit (ThermoFisher Scientific, AM1931) according to the instructors manual. Residual genomic DNA was digested with RNase-free DNaseI (ThermoFisher Scientific, EN0521) and cDNA reverse transcribed with SuperscriptIII (ThermoFisher Scientific, 18080-093) while using oligo(dT)16-primer (ThermoFisher Scientific, 8080128). Quality of all prepared totalRNA-extractions was evaluated on a 2100 Bioanalyzer Instrument (Agilent Technologies, 5067-1511). Poly(A)-containing mRNA molecules from totalRNA-samples were purified with oligo(dT)-magnetic beads, subsequently fragmented and cDNA synthesised with random primers using the TruSeq RNA Sample Preparation Kit v2. Adapter ligation and PCR-amplification precede cluster formation with a cBot cluster generation system.
identifiers.namespace: GEO
identifiers.id: GSM4123893