title: GSM2402544: Lgr5+EpCAM+_E11.5_intestinal epithelium_Id2KO_rep3; Mus musculus; RNA-Seq
library_construction_protocol: 250 EpCAM+, Lgr5+EpCAM+, EpCAM- (mesenchymal) embryonic intestinal cells and Lgr5-EGFPhigh ISCs were isolated by FACS directly in 7ml of lysis buffer (Clontech) supplemented with 5% RNase inhibitor and stored at -80°C. For each replicate, RNA was isolated from either single embryos (E11.5) or pools of 6–12 embryos (E13.5). PolyA+ mRNA was used for cDNA synthesis using SMARTer v3.0 kit (Clontech) according to manufacturer’s instructions. Amplification was performed for 15 cycles. After cDNA fragmentation (Covaris), libraries were prepared using Ovation Ultralow Library System (NuGEN) according to manufacturer’s instructions. RNA-seq, single end 50 bp
identifiers.namespace: GEO
identifiers.id: GSM2402544