title: GSM3011529: NTX9415sc_67; Homo sapiens; RNA-Seq
library_construction_protocol: Single CXCR4+MET+CD44+ cells were sorted into 96-well plates into lysis buffer. SuperScript II double-stranded cDNA Synthesis kit (Thermo Fisher) was used for reverse transcription. ERCC spike-in (Thermo Fisher) was added to the primer mix. AmPure XP kit (Beckman Coulter) was used for RNA clean-up. In vitro transcription was performed using the MEGAscript T7 transcription kit  (Thermo Fisher). aRNA was transcribed to cDNA using random hexamer primers and SuperscriptII polymerase (Invitrogen) and amplified using RP1 Illumina Primers.
identifiers.namespace: GEO
identifiers.id: GSM3011529