title: GSM2902811: ddm1a/1b/drm2 BS-seq; Oryza sativa Japonica Group; Bisulfite-Seq
library_construction_protocol: Rice seedling total RNA samples were isolated using TRIzol reagent (Invitrogen). For genomic BS-seq, total genomic DNA of callus culture-regenerated wild-type and mutant plants was extracted from 12-d-old seedling leaves using the DNeasy plant mini kit (Qiagen). For nuclear extraction, seedling leaf of plant on 1/2MS mediun for 12-day were ground into powder in liquid nitrogen, The resulting powder was suspended in nuclear isolation buffer (NIB; 20 mM Tris-HCl, 50 mM EDTA, 5mM Spermidine, 0.15 mM Spermine, 0.1% mercaptoethanol,40% Glycerol at pH 7.5) and followed the standard protocol for nuclei isolation. libraries were constructed following standard procedure of TruSeq Sample Preparation Kit from Illumina
identifiers.namespace: GEO
identifiers.id: GSM2902811