title: Metagenomics of compost and klasmann peat
design: Variable regions V1 (175 bpinsert), V3 (259 bp insert), V6 (168 bp insert) and V1-V2 (416 bp insert) of the prokaryotic 16S rRNA gene were amplified from gDNA obtained from the isolates. Variable regions ITS5-ITS2 (306 bp insert) and ITS3-ITS4 (385 bp insert) of the eukaryotic 18S rRNA gene were amplified from gDNA obtained from the isolates.  PCR amplification products were used to create a library using the Ion Plus Fragment Library Kit (Cat. No. 4471252) with sample indexing using the Ion Xpress™ Barcode Adapter 1-96 Kit. Template preparation was performed using the Ion OneTouch™ 2 System and the Ion PGM™ Template OT2 400 Kit (Cat. No. 4479878). Sequencing was performed using the Ion PGM™ Sequencing 400 Kit (Cat. No. 4482002) on the Ion PGM™ System using the Ion 318™ Chip v2 (Cat. No. 4484355) with all barcoded samples on a single chip. Primary data analysis was performed with Torrent Suite™ Software v4.0.2.
identifiers.namespace: CEBAS-CSIC
identifiers.id: A7-A9