title: GSM3609984: NRAS-G12D+ MPP mouse 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was obtained using Picopure RNA isolation kit (Arcturus) RNA amplification: RNA was amplified and prepared for RNA-Seq using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Library preparation: The RNA sequencing library was prepared with the Low Input Library Prep Kit v2 (Clontech) to construct index-tagged cDNA. The quality, quantity and the size distribution of the Illumina libraries were determined using the Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical). Sequencing: Libraries were sequenced on the Illumina HiSeq 2500 (Illumina) following the standard sequencing protocol with the TruSeq SBS Kit v4 and the following software versions: HCS 2.2.58, RTA 1.18.64
identifiers.namespace: GEO
identifiers.id: GSM3609984