title: GSM2061653: T2-1; Limacina inflata; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Plus Micro Kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were assessed using a NanoDrop ND-1000 spectrophotometer and denaturing gel electrophoresis using standard methods (Sambrook & Russel, 2001). Before being shipped on dry-ice to the Macrogen sequencing facilities in Seoul, South Korea, each RNA sample was precipitated in ethanol and sodium acetate (2x and 0.1x sample volume, respectively), and stored at -80 °C. Library construction was performed by Macrogen (South Korea). The libraries were sequenced using the Illumina HiSeq2000 platform, producing an average of 63 million sequence reads (100 bp paired-end) per sample.
identifiers.namespace: GEO
identifiers.id: GSM2061653