title: GSM1401476: 9wk_101; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA was extracted using a Qiagen all-prep DNA/RNA kit RRBS libraries were constructed using the Truseq DNA LT kit, with a bisulfite treatment after adapter ligation. For locus specific bisulfite sequencing, we bisulfite treated DNA and PCR amplified regions of interest using primers with a 5' tail. A second PCR recognizing the 5' tail added barcode and adapter sequences.
identifiers.namespace: GEO
identifiers.id: GSM1401476