title: GSM1891638: RNA_TKO_NRF1_KD_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated with the RNeasy Mini kit (Qiagen) with on-column DNA digestion. Two micrograms of total RNA were depleted from ribosomal RNA using the Ribo-Zero rRNA removal kit (Epicentre). Library Construction Protocol: Strand-specific libraries for RNA-seq were prepared using the ScriptSeq v2 protocol (Epicentre). Quality of the libraries and size distribution was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing was performed on an Illumina HiSeq 2500 machine (50 bp read length, single end) according to Illumina standards.
identifiers.namespace: GEO
identifiers.id: GSM1891638