title: GSM3990029: WT_K27me3; Mus musculus; ChIP-Seq
library_construction_protocol: Mouse hair follicle stem cells were purified from Sox9-CreER;NFIBfl/fl;NFIXfl/fl and WT littermate mice at P70 2nd telogen. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies (H3K4me1, Abcam, #ab8895; H3K27ac, Abcam, #ab4729; H3K27me3, Millipore, #07-449). Libraries were prepared according to a ChIPmentation protocol (Schmidl et. al., Nature Methods, 2015 ). Briefly, immunoprecipitated nucleosomes on beads were tagmented by Tagmentation enzyme from the Nextera DNA Sample Prep Kit (Illumina). Following tagmentation, the beads were washed and DNA elution, reverse cross-linking, DNA purification, and PCR amplification were performed. During PCR amplification, each sample were barcoded by different adaptor primers for Illumina sequencing. Sequencing libraries were pooled and sequenced on the NextSeq 500 instrument with 37/38 cycles paired end high-output mode.
identifiers.namespace: GEO
identifiers.id: GSM3990029