title: GSM1701102: RISC-associated small RNA from NoDice(4-25) expressing truncated human Dicer N1 infected with Polio Virus Sabin 1; Homo sapiens; Human poliovirus 1 strain Sabin; RIP-Seq
library_construction_protocol: RISC-bound miRNAs 16 h postinfection were isolated by immunoprecipitation using a monoclonal antibody that recognizes all human Argonaute proteins (Abcam: AB 57113), and proteins were removed by digestion with proteinase K. Recovered small RNAs were then isolated using a mirVana kit (Ambion). The immunoprecipitated RNA (RIP-Seq) cDNA library was constructed using an Illumina TruSeq small RNA kit. All small RNA input was processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.
identifiers.namespace: GEO
identifiers.id: GSM1701102