title: GSM2481346: E95repl_ElemC; Mus musculus; OTHER
library_construction_protocol: Multiplexed samples were sequenced on the Illumina Genome HiSeq 2500 system using 100 bp single-end reads according to the manufacturer’s specifications. 4C-seq reads were sorted, aligned, and translated to restriction fragments using the 4C-seq pipeline of the BBCF HTSstation (David et al. 2014, Plos ONE and available at http://htsstation.epfl.ch). Samples were mapped to the ENSEMBL Mouse assembly NCBIM37 (mm9). Illumina adapters (single end reads) were included in the inverse 4C PCR primers used for 4C-seq library preparation Cells were isolated in PBS supplemented with 10% Fetal Calf Serum. Tissue sample were further made single cell by collagenase treatment and applying a cell-strainer cap (BD Biosciences). Chromatin was crosslinked in the presence of 2% formaldehyde. PCR amplification using primers with included adapter sequences (for Illumina HiSeq2500)
identifiers.namespace: GEO
identifiers.id: GSM2481346