title: GSM3384416: iOL_Seh1_ChIP-Seq; Rattus norvegicus; ChIP-Seq
library_construction_protocol: Briefly, for ChIP-Seq, approximately 5 x10^6 cells were cross-linked with 1% formaldehyde for 10 min at room temperature and then were quenched with 125 mM glycine for 5min. Sonicated chromatin was used for immunoprecipitation by incubation with appropriate antibodies (4 g) overnight at 4°C. Immunoprecipitated complexes were collected using protein 40l A/G plus agarose beads (Millipore). SubSequently, beads were washed Sequentially with low-salt buffer, high-salt buffer, LiCl buffer and twice with TE and elution in 500 l of elution buffer (1% SDS, 0.1M NaHCO3). The elutes were heated at 42°C for 2h and treatment with proteinaseK followed by 65°C 10 h to reverse the cross-linking and were treated  with RNaseA for 30 min before DNA was extracted and purified. The ChIP libraries were prepared using KAPA HyperPrep Kits (Roche, 7962347001)
identifiers.namespace: GEO
identifiers.id: GSM3384416