title: GSM4182141: GmMM_GLYMA.08G357600-B-ChIP_br1.2; Glycine max; ChIP-Seq
library_construction_protocol: Chromatin isolation and immunoprecipitation were performed as described in Gendrel AV, Lippman Z, Martienssen R, Colot V. Nat Methods. 2005;2:213–218, except that the isolated nuclei were resuspended in lysis buffer described in by Johnson et al. (2002) and sonicated to achieve chromatin fragments ranging from 100 to 500 bp.  Nuclear extracts were either set aside for whole genome extracts (input control) or incubated overnight with 3ug of an an antibody raised against the peptide QQNPDPGLGGTVGEC, corresponding to the amino acids 118-132 of GLYMA.08G35760.  Crosslinks were reversed and protein digested as described in Dahl and Collas, Nat Protoc. 2008;3(6):1032-45, extracted with phenol:chloroform and precipitated using standard procedures. ChIP libraries were generated using Nugen Ovation Ultralow DR Multiplex System, following the manufaturer's instructions and enriched by PCR using 15 cycles.  Libraries were size-selected by electrophoresis and purified using Qiagen MinElute gel purification kit
identifiers.namespace: GEO
identifiers.id: GSM4182141