title: T. oceanicus head tissue RNA sequencing
design: The GS FLX Titanium Sequencing Kit XLR70 (Roche) was used. Libraries were synthesized using random primer synthesis (EMD4Biosciences). The technique utilized EcoR I and Hind III linkers to create priming sites at the 3’ end of single-stranded mRNA transcripts. Double-stranded cDNA was synthesized using these primers, and subsequent digestion with EcoR I and Hind III produced fragments that were then ligated onto customized 454 adapters for the sequencing reaction. The resultant libraries were size-selected using Ampure XP beads (Agencourt) and Sizing Solution (Roche) according to the GS-FLX Titanim cDNA rapid library preparation standard method (Roche). Libraries were not normalized prior to sequencing, so read numbers reflected transcript abundance.
identifiers.namespace: University of St Andrews
identifiers.id: T. oceanicus head tissue RNA sequencing