title: GSM4671873: Mll3KOMll4KI27_D0_ATAC; Mus musculus; ATAC-seq
library_construction_protocol: 50,000 freshly collected cells were incubated in 50 µL of cold lysis buffer (10mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 0.1% Tween-20, and 0.01% digitonin) on ice for 3 min and was washed out with 1 mL of wash buffer (10mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20). Nuclei were collected by centrifugation at 500 g for 10 min at 4°C, then resuspended in 50 µL of transposition reaction buffer (25 µL 2x Tagment DNA buffer, 2.5 µL Tn5 transposase (100nM final; Illumina), 16.5 µL PBS, 0.5 µL 1% digitonin, 0.5 µL 10% Tween-20, 5 µL H2O). The reaction was incubated at 37°C for 30 min with mixing (1000 rpm) and directly subjected to DNA purification using the MinElute Reaction Cleanup Kit (Qiagen) according to the manufacturer's instructions. DNA was amplified with PCR using Nextera i7-index primers for Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM4671873