title: GSM1890899: Liver_1_ZT02; Mus musculus; RNA-Seq
library_construction_protocol: RNA preparation using the Ambion PureLink RNA Mini Kit (Life Technologies) Briefly, fractions were precipitated by the addition of three volumes of ethanol and kept overnight at 280uC. After 30 min of centrifugation at 5,200 g, RNAs were extracted from the non- soluble fraction by classical protocol. Liver RNAs were extracted and analysed by real-time quantitative RT-PCR, mostly as previously described(Jouffe et al 2013). Briefly, 0.5 mg of liver RNA was reverse transcribed using random hexamers and SuperScript II reverse transcriptase (Life Technol- ogies). The cDNAs equivalent to 20 ng of RNA were PCR amplified in triplicate in an ABI PRISM 7700 Sequence Detection System (Applied Biosystem) using the TaqMan or the SYBR Green technologies Illumina TruSeq RNA Libraries for ultra-high throughput sequencing were prepared with the RNA-seq sample kit (Illumina) as recommended by the manufacturer.
identifiers.namespace: GEO
identifiers.id: GSM1890899