title: GSM2566912: Exp 3 Treated rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from 1x10^5 – 1x 10^6 cells using the RNeasy® Plus Mini kit (Qiagen, Germany) according to the manufacturer’s directions. RNA was eluted from the RNeasy spin column in 30 μL of RNase-free H20 and precipitated in 2x volume 100% ethanol and 1/10x volume NaOAc for 30 min at -80ºC. Precipitated RNA was then centrifuged at 12 000 x g for 30 min at 4ºC, the pellet washed in 75% ethanol and then centrifuged at 8000 x g for 30 min at 4ºC. Supernatant was removed, RNA was air dried and resuspended in 30 μL of RNase free water. mRNA libraries were prepared at the Ramaciotti Centre for Genomics (UNSW University of New South Wales, Australia). The Illumina TruSeq Stranded Total RNA Prep Kit was used for Experiments 3. The six RNA-Seq libraries in Experiments 2 were sequenced using the Illumina NextSeq 500. R1.fastq and R2.fastq files were produced for each sample.
identifiers.namespace: GEO
identifiers.id: GSM2566912