title: GSM4672382: S. cerevisiae Aligned to C. albicans Genome; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells encapsulation into nL droplets using a microfluidic device along with oligo poly-dT beads and zymolyase containing lysis buffer. Cells were then incubated at 37°C for 15-25 minutes for lysis. mRNA was captured by the poly-dT oligo beads. Reverse tracnription to cDNA was performed using a template switch oligo. Beads then underwent exonuclease treatment and PCR amplification. A Illumina Nextera (cat#FC-131-1096) was used with 500-600 pg cDNA for construction of sequencing libraries.
identifiers.namespace: GEO
identifiers.id: GSM4672382