title: Bacteria
design: DNA was extracted from each 0.5 g (fresh weight) soil sample with the CTAB/SDS method, using MP FastDNASPIN Kits for soil (MP Biomedicals, Solon, OH, USA). After concentration and purification, extracted DNA was diluted to 1 ng/l using sterile water. We then amplified the V4-V5 hypervariable regions of the 16S rRNA gene using the primers 515F (5-GTG CCA GCM GCC GCG GTA A-3) and 806R (5-GGA CTA CHV GGG TWT CTA AT-3) for bacteria
identifiers.namespace: SUB6257865
identifiers.id: 10