title: GSM3610797: T2D_Dementia: B40910; Homo sapiens; Bisulfite-Seq
library_construction_protocol: T2D_Dementia: As part of the Israel Diabetes and Cognitive Decline (IDCD) study whole blood was collected from subjects within serum tubes and stored on ice for a maximum of 6 hours before laboratory processing. The blood tubes were centrifuged (10 minutes, 1,500 rpm) at room temperature. Serum was then pipetted from top and stored at -80C. All subjects were diagnosed with Type 2 diabetes. Cell-free DNA extraction from plasma or serum was performed using the Analytik Jena PME free-circulating DNA Extraction Kit (845-IR-0003050) using the SE/SBS system only. Bisulfite Conversions were performed using the MethylCode Bisulfite Conversion Kit (Invitrogen, MECOV50). Libaries were constructed using the Nextera transposase (Tn5) enzyme supplied within Illumina Nextera® DNA Sample Preparation Kit (FC-121-1031).
identifiers.namespace: GEO
identifiers.id: GSM3610797