title: GSM4061925: Human atrial tissue sample in age group SR60, replication 2; Homo sapiens; miRNA-Seq
library_construction_protocol: The total RNA was extracted from the frozen human right atrial appendages using TRIZOL reagent following the manufacturer's protocol. The RNA yield and purity were determined using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA) and NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, USA). Genome-wide miRNA expression profiles of twelve human right atrial tissues were generated by miRNA-seq, which was carried out at the Biodynamic Optical Imaging Center (BIOPIC) in Peking University (Beijing, China). miRNA-seq followed the BIOPIC recommended protocol that was based on the NEBNext Multiplex Small RNA Library Prep Kit (Lot #E7560S). Each library was sequenced in two lanes.
identifiers.namespace: GEO
identifiers.id: GSM4061925