title: GSM4176378: C3; Homo sapiens; Bisulfite-Seq
library_construction_protocol: After tissue homogenization, DNA was extracted according to the instructions of TIANamp Genomic DNA Kit (Cat#DP304) A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research), before the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X). Library concentration was quantified by Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system.
identifiers.namespace: GEO
identifiers.id: GSM4176378