title: GSM2399703: stripped root 25ºC rep2; Glycine max; Bisulfite-Seq
library_construction_protocol: Root hairs and stripped roots were isolated after 3 hours of incubation as described by Wan et al. (2005). Genomic DNA was extracted using the DNeasy® Plant Mini Kit (Qiagen) following the manufacturer's instructions. One µg of gDNA was spiked with 5ng of unmethylated Lambda DNA (Promega), and randomly sheared to an average of 300 bp using the Covaris S220 instrument. Sheared DNA was end-repaired, A-tailed and ligated using TruSeq DNA sample preparation kit (Illumina, San Diego, CA). Bisulfite treatment was performed twice using the EpiTect Bisulfite kit (Qiagen) following the manufacturer's protocol for DNA isolated from formalin-fixed paraffin-embedded tissue (FFPE) tissue samples. The bisulfite-converted library was amplified (9 cycles) by PCR with KAFA HiFi Uracil+ DNA polymerase (Kapa Biosystems) according to the manufacturer's conditions.
identifiers.namespace: GEO
identifiers.id: GSM2399703