title: GSM4277990: Abl1_RRBS_med_5azadC_rep3; Mus musculus; Bisulfite-Seq
library_construction_protocol: 2x10^5  cells were spun down, washed using PBS and flash frozen on dry ice for DNA extractions for RRBS. Dead cells were removed from these samples by sucrose gradient centrifugation. (Histopaque®-1077, Sigma, Cat 10771), before sample preparation using conditions specified by the manufacturer. Libraries were generated from 50ng input DNA using a scaled-down (half reactions) of the NuGEN Ovation RRBS Methyl-Seq System (Tecan) following the manufacturer's recommendation. Libraries were PCR amplified for 11 cycles.
identifiers.namespace: GEO
identifiers.id: GSM4277990