title: RNA-Seq of Rattus norvegicus: fetal (gestational day 20) cardiac tissue from             nano-Tio2 gestational exposed offspring
design: ardiac tissue was procured through the same methods as listed above                 in the ChIP Sequencing section. RNA was then isolated from heart tissue using the                 Vantage Total RNA Purification Kit (Origene, Rockville, MD) per manufacturers                 instructions. Briefly, tissue was homogenized and lysis buffer was added to the                 sample. Sample RNA was spin-column purified and measured for RNA concentration using                 the Qubit (Thermo Fisher, Rockford, IL). Library preparation was performed using                 TruSeq RNA Library Prep Kit v2 (Illumina, Inc., San Diego, CA). Quality of RNA was                 determined using the Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara,                 CA)