title: GSM2671415: mm10_e16; Mus musculus; RNA-Seq
library_construction_protocol: Murine LGs from timed-pregnant female mice (CD1; E16 and P4) were dissected and pooled together in PBS. A single cell suspension was created by incubating lacrimal glands in a PBS solution containing Liberase TM (Roche) and DNaseI (Roche) at 37 C for 20-40 min. The enzyme reaction was quenched by the addition of fetal calf serum and the solution was filtered through a 40 um strainer (BD Falcon) and centrifuged at 1500 rpm for 5 mins. Cell pellets were washed in PBS, centrifuged and resuspended in 0.04% BSA (ThermoFisher) at a cell density of 1000 cells/uL. Isolate single cells for Drop seq technology to barcode.
attributes.tag: GEO Accession
attributes.value: GSM2671415