title: Illumina HiSeq 2000 sequencing; qiita_ptid_73:632.Arctic.Tundra.2
design: In an effort to enable the sharing of genetic material from environmental samples for the benefit of the scientific community, we are establishing the Canadian MetaMicroBiome Library. This repository will house composite soil samples that are characterized by extensive 16S rRNA gene and whole-genome sequencing, generating taxonomic profiles and metabolic pathway reconstructions for each sample. The sequence data will be submitted to existing public databases and linked with detailed physical, chemical and geographic characterization of the samples according to established metagenomic standards. Sample DNA will be used to construct high quality libraries in cosmid vectors, allowing for phenotypic screening in a broad range of microbial surrogate hosts. Phenotypic screening of metagenomic libraries provides access to truly novel functions that would otherwise be missed by sequence-based surveys of bulk community DNA or metagenomic libraries. MetaMicroBiome is being initiated with soil samples collected from across Canada, and initial libraries are being screened for novel glycoside hydrolase activities in multiple surrogate hosts. Once MetaMicroBiome is established, contributions of samples from other environments will be accepted for inclusion. The resulting resource will provide the international scientific community with access to a common collection of thoroughly characterized community-contributed genetic material in clone library format for custom phenotypic screen
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: GTTCTCTTCTCG
attributes.tag: center_name
attributes.value: U Waterloo
attributes.tag: center_project_name
attributes.value: Canadian MetaMicroBiome Initiative
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: HiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 9/1/11
attributes.tag: run_prefix
attributes.value: s_3_1_sequences
attributes.tag: samp_size
attributes.value: .1,g
attributes.tag: sample_center
attributes.value: LBNL
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4