title: RNA-seq of NGR234: Phaseolus nodules 1
design: The seeds of Phaseolus vulgaris were germinated and planted as described by Skorpil et al., 2005, and then inoculated with Sinorhizobium fredii NGR234. Plants were grown at a daytime temperature of 24C, a night temperature of 20C and photoperiod of 16 hours. Nodules were harvested 21 days after inoculation and stored at -80C until total RNA extraction. After rRNA-depletion and plant RNA-depletion, bacterial mRNA-enriched samples were used to construct the libraries.