title: GSM3014814: Prdm9-mut 16dpp rep3; Mus musculus; RNA-Seq
library_construction_protocol: The procedure was as previously described with some modifications (Cobb 1997, La Salle 2009). Briefly, seminiferous tubules were transferred into 20 ml DMEM (Gibco, Life Technologies, Carlsbad, CA, USA) containing 0.5 mg of Liberase TL Research Grade (05401020001, Roche, Basel, Switzerland) and incubated for 20 min at 32 °C. To remove interstitial cells, tubules were washed three times with the same media. Isolated germ cell samples were resuspended in QIAzol Lysis Reagent according to the manufacturer's instructions, and total RNAs were purified from homogenized cells using Qiagen RNeasy Mini Kit (74104).
attributes.tag: GEO Accession
attributes.value: GSM3014814