title: GSM1287735: WT ESC -2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol (Invitrogen) according to the manufacturers’ directions. RNA quality was determined using BioAnalyzer (Aligent). RNA integrity numbers above 7.5 were deemed sufficiently high quality to proceed with library preparation RNA sequencing libraries were generated from ~250 ng total RNA using the illumina TruSeq RNA kit v2, according to the manufacturers' directions. Libraries were sequenced at the Broad Institute genomics platform on a HiSeq 2500 or at the Harvard Northwest labs sequencing facility on a HiSeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM1287735