title: GSM2670987: RNA pol II Ser2-P ChIP-seq Cdk7as Rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: cross-linked Chromatin was sonicated with a Bioruptor 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, gel purification of ~200bp fragments, and amplification (18cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.
attributes.tag: GEO Accession
attributes.value: GSM2670987