title: GSM3926023: 3D24h-Expansion RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: For cells cultured in vitro, samples were harvested, and RNA extraction was performed using RNeasy Plus Micro Kit (Qiagen). The total RNA was then subjected to the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to the manufacturer's instructions. For samples harvested in vivo, proper amount of tissues was collected under the mice skins and then homogenized in Trizol with the tissue pellet pestle. Total RNA was then extracted following the Trizol RNA extraction protocol. In brief, every 50-100 mg tissue was homogenized in 1 mL TRIzol then 0.2 mL chloroform. The aqueous phase (with RNA) was collected and pelleted with isoproponal precipitation. We treated the extracted total RNA with DNase to remove residual DNA in the samples. RNA libraries were prepared for sequencing using Illumina TruSeq stranded mRNA kit.
attributes.tag: GEO Accession
attributes.value: GSM3926023