title: WGS of Phaseolus vulgaris: Variety MIDAS
design: DNA was extracted from freeze-dried bean leaves of greenhouse-grown plants using a modified protocol of Pallotta et al. (2003) with an extra step consisting in re-suspension with 4 μl of RNAse and incubation for 30 minutes at 37 0 C. DNA quality was checked with NanoDrop Lite (Thermo Fisher Scientific) and by 1% agarose gel electrophoresis. DNA with an absorbance ratio (A260/A280) > 1.7 and with no visible degradation on agarose gel was used for subsequent library preparation.