title: GSM1703690: Control-3_RNA-Seq; Rattus norvegicus; RNA-Seq
library_construction_protocol: [Tissue collection] The terminal half of the IM (IM tip) was dissected from kidney and used for RNA preparation. [RNA preparation] The total RNA from rat IM tip was extracted using Trizol Reagent (Invitrogen). The RNA samples were purified using an RNeasy Mini Kit (Qiagen). Quantification and purity assessment of the RNA samples were determined on a NanoDrop Spectrophotometer (Nano-Drop Technologies).  RNA quality was assessed with an Agilent Bioanalyzer 2100. [cDNA preparation] Equal amounts of purified mRNA was transcripted to cDNA using a SMARTer PCR cDNA Synthesis Kit (Clontech Cat#634925). The cDNAs (Ctrl n=3, STZ n=3) for high-throughput sequencing were fragmented by DNase I and ligated to Illumina adapters. These adapter-ligated cDNA fragments were amplified and sequenced on the Illumina HiSeq2000 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM1703690