title: GSM4183037: CRISPRi Library H6 high peak; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was isolated from FACS-sorter populations using QIAamp DNA Blood Maxi kit. Genomic DNA was PCR amplified with Q5 Hotstart high-fidelity 2X mastermix and barcoding primers for 27 cycles. PCR reactions were purified on QIAquick PCR purification columns and loaded onto a 20% polyacrylamide/TBE gels. A product band ~270 bp was excised and DNA was extracted, quantified and sequenced on an Illumina HiSeq-4000 using custom primers
attributes.tag: GEO Accession
attributes.value: GSM4183037