title: PacBio 16S rRNA gene sequence data: Pooled sample: Sample V1V3_june
design: " ""There are 4 samples present in the PacBio Run and the V1V3 region from each sample was sequenced in triplicate. The data contains a human gun metagenome, a mouse gut metagenome, a soil metagenome, and a synthetic metagenome. In order to demultiplex the data you will need to use the following primers (AGRGTTTGATYMTGGCTCAG ATTACCGCGGCTGCTGG) and the sample and barcode information. mock1 (TGTGTATCAGTACATG CATGTACTGATACACA), mock2 (ACACGCATGACACACT AGTGTGTCATGCGTGT), mock3 (GATCTCTACTATATGC GCATATAGTAGAGATC), mouse1 (ACAGTCTATACTGCTG CAGCAGTATAGACTGT), mouse2 (ACAGTCTATACTGCTG CAGCAGTATAGACTGT), mouse3 (CTGCGTGCTCTACGAC GTCGTAGAGCACGCAG), human1 (TCAGACGATGCGTCAT ATGACGCATCGTCTGA), human2 (CTATACATGACTCTGC GCAGAGTCATGTATAG), human3 (TACTAGAGTAGCACTC GAGTGCTACTCTAGTA), soil1 (GCGCGATACGATGACT AGTCATCGTATCGCGC), soil2 (CGCGCTCAGCTGATCG CGATCAGCTGAGCGCG), soil3 (GCGCACGCACTACAGA TCTGTAGTGCGTGCGC). These data were generated in June 2015 and are from Run 0006. The P6C4 chemistry was used to generate these data."""