title: GSM3993372: Hs_H1_WT_D8-14-miR-poly_RNAseq_1; Homo sapiens; RNA-Seq
library_construction_protocol: mRNA was extracted using miReasy mini kit from Qiagen. Libraries were prepared using the protocol from Dr. Sander lab. Briefly, RNA were first poly A selected and then subject to RNA fragmentation. Then fragmented RNA were proceeded with first stand synthesis, second strand synthesis, end repair and A-tailing. Resulting product was ligated with adaptor and selected by size, and next were enriched using PCR. At last DNA purification was performed and samples sent out for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3993372