title: GSM1946346: longissimus dorsi  rep3_180d-3; Sus scrofa; miRNA-Seq
library_construction_protocol: The longissimus dorsi muscle tissues from the same area were collected at E90, 0, 30, 180 days and 7 years. Subsequently, the samples were immediately frozen in liquid nitrogen and stored at −80°C until RNA extraction. Total RNAs were isolated from each sample using Trizol (Takara) according to the manufacturer’s protocol. The small RNA fraction between 10-40 nt was isolated by polyacrylamide gel electrophoresis (PAGE) and ligated with proprietary adaptors (Illumina). The short RNAs were then converted to cDNA by RT-PCR and each small RNA library was sequenced individually using Illumina Genome Analyzer II according to manufacturer instructions.
attributes.tag: GEO Accession
attributes.value: GSM1946346