title: RNA-seq of Ambrosia artemisiifolia female flower
design: 2.0 ?g of total RNA from 1F and 2F samples was used as starting material for cDNA synthesis and library construction. Enrichment of mRNA, cDNA synthesis and library preparation for Illumina NextSeq paired-end sequencing were carried out using TruSeq™ RNA sample preparation kit (Low-Throughput protocol) according to manufacturer's instructions.