title: 16S rRNA genes of bacterial communities: Ohio River
design: Tagged 16S rRNA gene V4 variable region primers 515f and 806r were used in a single step 30 cycle PCR using the HotStar Taq Plus Master Mix Kit under the following conditions: 94°C (3 minutes), followed by 28 cycles of 94°C (30 seconds), 53°C (40 seconds), and 72°C (1 minute), and a final elongation step at 72°C (5 minutes).