title: miRNA-seq of Glossina pallidipes: wild type male whole body
design: Method: Tissue miRNA profiles of wild type males were generated using high throughput sequencing by illumina Hiseq 2500. The sequences that passed quality check were analyzed using miRDeep2 V2.0.08: the reads were aligned to the Glossina pallidipes genome (build GCA_000688715.1) using Bowtie and followed by statistical analysis using Perseus and Empirical Analysis of Digital Gene Expression Data v3.16.5 (edgeR) package in R