title: DRIP: Human: K2_6mA m6A DRIP performed on REBL-PAT hPSCs
design: Sequencing libraries were prepared according to the NEB Next DNA Ultra Library Preparation Kit for Illumina (NEB, E7370). DNA was sonicated to 400-600bp (Covaris S2) and adapters were ligated (NEB, E73355S) according to the protocol. Adapter ligated DNA was digested with USER enzyme as stated in the protocol. Following immunoprecipitation, the enriched adapter ligated DNA was amplified for 15 cycles and libraries were quantified using the Kapa Library Quantification Kit (Kapa Biosystems, KK4823). Sequencing was performed using the Illumina NextSeq500 platform to generate 2 x 150 bp reads.