title: GSM1948690: CK_replicate 2; Solanum lycopersicum var. cerasiforme; RNA-Seq
library_construction_protocol: Total RNA samples were prepared using the Total RNA Purication Kit, TRK1001 (LC Science, Houston, TX), treated with RNase-free DNaseI following the manufacturer's procedure to remove contaminated genomic DNA. The quantity and purity of total RNA were analysed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA).Equal quantities of high-quantity RNA of each sample were blended for cDNA library construction. The poly (A) messgener RNA was isolated from the total RNA samples with oligo (dT) attached magnetic beads (Invitrogen). The mRNA was frangmented into short pieces using divalent cations under elevated temperature. The cleaved RNA fragments were reverse-transcribed to the first-strand cDNAs by random hexamer primers. Then the second-strand cDNAs were synthesized to construct the final cDNA library according to the instruction of mRNA-Seq sample preparation kit (Illumina, San Diego, USA) and the paired-end cDNA with a length of 300 bp (±50 bp). After that, the cDNA libraries were sequenced on the Illumina HiSeqTM 2000 platform at the LC-BIO (Hangzhou, China) following the manufacturer’s recommendations.
attributes.tag: GEO Accession
attributes.value: GSM1948690