title: GSM3611633: REU4502A23 (RN108); Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA was extracted from hippocampus tissue using TRI reagent as per manufacturer's instructions. The resulting RNA pellets were dissolved in 50 μl of nuclease-free water (Ambion). Purified RNA was treated with DNase I (Qiagen, Germany) and incubated for 10 min at room temperature. DNase was inactivated by the addition of EDTA (Final conc. 5mM) for 10 min at 70°C and RNA was purified over MinElute spin column as per manufacturer's instructions (Qiagen, Germany). Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal Kit (Epicentre/Illumina, Human/Mouse/Rat). Library preparation was completed using TruSeq Stranded Total RNA kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3611633