title: GSM3014845: wt_t4/t5_input_1 L3; Drosophila melanogaster; RIP-Seq
library_construction_protocol: 500 L3 larvae of the desired genotype were dissect to collect 500 Brains. The Brains were deep frozen in liquid nitrogen and then homogenized. A 10% of the brain homogenates was used as input, the rest was immunuprecipated with anti-GFP antibodies to isolate t4/t5 ribosomes. The RNA bound to the ribosome was then purified, sonicated and sequenced.
attributes.tag: GEO Accession
attributes.value: GSM3014845