title: GSM3856226: Ako1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA of sorted cells was extracted with quickExtract DNA Extraction Solution (Epicentre, QE09050) and the sgRNAs were amplified by two-step PCR method. For the second PCR, illumine adaptors and barcode were attached to samples. Amplicons from the second PCR were gel-extracted, quantified, mixed and sequenced using pair-ended 150bp sequencing protocol (Illumina Hiseq-x-ten system).
attributes.tag: GEO Accession
attributes.value: GSM3856226