title: GSM3014742: BB3_embryo_230816_ACAGTG; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Synchronized Embryos or L4 larval worms were washed several times to avoid contamination with bacteria, snap-freezed at liquid nitrogen, and grinded to powder in a liquid nitrogen -prechilled mortar and pestle. High-molecular weight and Low-molecular weight RNA fractions were isolated using miRVana miRNA isolation kit Applied Biosystems (Ambion). libraries were prepared from the high molecular weight fraction by means of SMARTer® Stranded RNA-Seq Kit (Clontech Laboratories) after ribosomal depletion by Ribozero kit (Epicenter)
attributes.tag: GEO Accession
attributes.value: GSM3014742