title: GSM3613345: ATOH1_1; Homo sapiens; ChIP-Seq
library_construction_protocol: The ChIP method was modified from protocols described in Schmidt et al.(Schmidt et al., 2009). MKL-1 cells were cross-linked using dual cross-linking with disuccinimidyl glutarate (DSG) and formaldehyde. After cross-linking, cells were lysed using SimpleChIP buffer A and B (Cell signaling) and DNA was processed with the Micrococcal nuclease (New England Biolabs Inc.) for 30 minutes at 37°C followed by sonicating for 20s pulses 5 times at 4°C. 30 ng of DNA from ChIP experiments or input DNA were prepared for sequencing with NEBNext ChIP-seq Library Prep Reagent Set for Illumina (New England BioLabs). Amplified libraries were cleaned up using AMPure XP beads (Beckman Coulter) and checked on a Bioanalyzer (Agilent) to confirm a narrow distribution with a peak size around 275 bp. Diluted libraries were used for 50 cycles single-end sequencing on NextSeq 550 systems  (Illumina) at the Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer Institute following the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM3613345