title: RNA-Seq of cassava storage roots
design: After extract total RNA and treated with DNase I, Oligo(dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA are fragmented. Then  cDNA  is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the  PCR  amplification. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time  PCR  System are used in quantification and qualification of the sample library. Then the library is sequenced using Illumina HiSeq 4000.