title: GSM2830245: Pseudomonas aeruginosa TPP Rep1; Pseudomonas aeruginosa; RNA-Seq
library_construction_protocol: Total RNA was extracted from candida albicans using hot acid phenol protocol.  Poly(A)+ selected mRNAs were obtained using Poly(A)purist kit from Ambion.  Total RNA was extracted from pseudomonas aeruginosa using Trizol.  Total RNA was extracted from bacillus subtilis using 1%SDS and lysozyme, followed by Trizol.  Ribosomal depletion on total bacillus and pseudomonas RNA was carried out using RiboZero kit for bacteria (Epicenter) Poly(A) enriched or ribosomal RNA depleted RNA were structured probed in the presence and absence of ligands using RNase V1.  The structure probed RNA was then fragmented, ligated to adapters, reverse transcribed and PCR amplified.
attributes.tag: GEO Accession
attributes.value: GSM2830245