title: RNA-Seq of late passage (p4) human chondrocytes cultured on devitalized synoviocyte matrix at physioxic (5%) oxygen tension, donor B
design: mRNAs were captured by oligo-dT magnetic beads and fragmented.  First strand cDNA was generated using random primers, and second strand cDNA was synthesized with dUTP.   The library was generated using the ds cDNA as template. Briefly, templates were end repaired, and a 3A was added before Y-shaped adaptors were added to each end. The strand with dUTP was then digested using UDG before PCR.