title: Seq of rDNA fungal and bacterial organisms on grape surface
design: Genomic DNA was sent to the Cornell University Sequencing facility in Ithaca, NY for sequencing library preparation and 2x250bp paired-end sequencing on an Illumina MiSeq sequencer (Illumina, San Diego, CA, USA). Dual-barcoded Nextera library preparation followed AmpSeq protocols [8] but with singleplex PCR. The V4 domain of bacterial 16S rRNA was amplified using primers (all sequences shown 5? to 3?): F515 (GTGTGCCAGCMGCCGCGGTAA) and R806 (GGACTACHVGGGTWTCTAAT).  Fungal internal transcribed spacer (ITS) 1 loci were amplified using primers BITS (CTACCTGCGGARGGATCA) and B58S3 (GAGATCCRTTGYTRAAAGTT) [1]. To enable sample barcoding, AmpSeq linkers were added to the 5? end of each locus-specific primer. As detailed in Yang et al., 2016, the linker to accommodate S5xx barcodes for each forward primer is: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG. The linker to accommodate N7xx barcodes for each reverse primer is: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG.