title: GSM4222585: sample_4; Mus musculus; RNA-Seq
library_construction_protocol: Each tooth was processed individually in the single-cell dissociation. The tooth germ was treated with 0.1 mg/ml liberase (Roche, Basel, Schwitzerland) in Dulbecco's solution for 15 min at 28°C in shaking at 300 rpm followed by gentle pipetting to detach the mesenchymal cells. Then the tissue preparation was treated with TrypLE Select (Life Technologies, Waltham, USA) for 15 min at 28°C in shaking at 300 rpm followed by gentle pipetting to detach the epithelial cells. The cells were washed once in PBS with 0,04% BSA. The cells were resuspended in 50 µl PBS with 0.04% BSA. Library prepartion was done according to the manufacturer's instructions in Chromium single cell 3' library & gel bead Kit v3 (10x Genomics, Pleasanton, USA).
attributes.tag: GEO Accession
attributes.value: GSM4222585