title: Ribo-seq of HEK293: control 2repl
design: Approximately 5 million HEK 293-TIA1 cells unstressed were used to isolate mRNA-bound ribosome complexes, followed by extraction of RNase I digestion-derived ribosome-protected fragments as described  {Ingolia, 2009} Cells were collected by flash-freezing without preincubation with antibiotics and the cDNA libraries from RPFs were prepared with direct ligation of the adapters and sequenced