title: Glomus Titanium Simple
library_construction_protocol: Aliquots of about 100,000 sterile G. intraradices spores from in vitro culture were activated in 6 ml of M medium containing 10-8 M GR24, a synthetic strigolactone, for 24h at 30 C, 2% CO2. Spores were immediately frozen in liquid nitrogen. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) with RLT lysis buffer. RNA quality and integrity were checked prior to cDNA synthesis using the Bio-Rad Experion analyzer and Experion RNA StdSens analysis kit. Total RNA preparations (three biological replicates) were amplified using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer's instructions. cDNAs were pooled after purification using QIAquick PCR purification Kit (Qiagen) and used for 454 pyrosequencing. The same three replicates of activated spores were used for oligoarray analysis.