title: GSM4223343: 931SATC; Ovis aries; RNA-Seq
library_construction_protocol: Both subcutaneous and visceral adipose tissue were collected during the second breeding season at ~21 months of age. Before collection, estrus was synchronized with two injections of prostaglandin F2α (PGF2α, 10 mg, i.m.; Lutalyse, Pfizer Animal Health, Florham Park, NJ) administered 11 days apart. Tissues were harvested 24 hours after the second PGF2α injection during the follicular phase to maintain comparable steroid environment. All animals were euthanized by barbiturate overdose (Fatal Plus; Vortech Pharmaceuticals, Dearborn, MI). Visceral adipose from the mesenteric fat surrounding the ventral sac of the rumen and subcutaneous adipose from the sternal region were obtained. Tissues were flash-frozen and stored at -80 °C until processed. Libraries were prepared with SMARTer universal low input RNA kit (Takara, Mountain View, CA) following ribosome depletion
attributes.tag: GEO Accession
attributes.value: GSM4223343