title: metagenomic analysis of T. chinensis in Yancheng
design: From the three hundred isopods collected, one representative individual from each of the three dierent locations was sent to Shanghai Personal Biotechnology Co., Ltd. for library construction and sequencing by paired-end mode through the Illumina Hiseq platform (Illumina, San Diego, USA). Total microbial genomic DNA samples were extracted using the DNeasy PowerSoil Kit (QIAGEN, Inc., Netherlands), following the manufacturers instructions, and stored at -20C prior to further assessment. The quantity and quality of extracted DNAs were measured using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. The extracted microbial DNA was processed to construct metagenome shotgun sequencing libraries with insert sizes of 400 bp by using Illumina TruSeq Nano DNA LT Library Preparation Kit. Each library was sequenced by Illumina HiSeq X-ten platform (Illumina, USA) with PE150 strategy