title: GSM4498324: ChIP-seq_H3K27ac_Bic/4AP; Mus musculus; ChIP-Seq
library_construction_protocol: Bisulfite-Seq; Genomic DNA was extracted using Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Nacalai Tesque). RNA-seq; Total RNAs were isolated with Sepasol-RNA I Super G (Nacalai Tesque), Total RNAs were purified using Poly (A) mRNA Magnetic Isolation Module (New England Biolabs). ChIP-seq; Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Bisulfite-Seq; Genomic DNA was subjected to bisulfite treatment with the EZ DNA Methylation-Gold Kit (ZYMO Research) according to the manufacturer's instructions. In this study, we adopted a strategy termed post-bisulfite adaptor tagging (PBAT), which provides an unbiased methylome analysis (Miura et al. 2012). The multiplex PBAT method is described in detail on the CREST/IHEC Japan webpage (http://crest-ihec.jp/english/epigenome/index.html) (Miura et al. 2012). RNA-seq; Purified mRNAs by polyA beads were used for library construction with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). ChIP-seq; Sequencing libraries were made using the NEBNext ultra II DNA Library Prep kit for Illumina (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM4498324