title: GSM3990109: Small RNA sequencing in pir2-1rrp6D cells; Schizosaccharomyces pombe; ncRNA-Seq
library_construction_protocol: Small RNAs between 15 and 30 nucleotides were extracted from 17% Urea-PAGE gels and eluted using Corning Costar Spin-X columns (Sigma Aldrich) followed by ethanol precipitation. NEBnext Small RNA Library for Illumina (NEB-E7330) was used according to the manufacturer's protocol Libraries were analyzed using an Agilent 4200 TapeStation system (Agilent) and sequenced on the Illumina MiSeq platform.
attributes.tag: GEO Accession
attributes.value: GSM3990109