title: Illumina HiSeq 4000 paired end sequencing; Transcriptional changes occurring in end-stage human SCA1 cerebellum.
design: Transcriptional changes occurring in end-stage human SCA1 cerebellum.
library_construction_protocol: Post-mortem human cerebellum from a 74-year-old female SCA1 patient and a age/sex-matched healthy individual were obtained from the MRC London Neurodegerative Diseases Brain Bank. Total RNA was extracted from frozen samples using the Direct-zol RNA Kit (Zymo Research). An aliquot of total RNA was loaded on Total RNA Pico Chip and measured for its integrity using Bioanalyzer 2100 (Agilent Technologies, USA). Sequencing libraries were prepared using KAPA RNA HyperPrep with Riboerase Kit, according to manufacture's instructions (Roche, USA).
attributes.tag: Experimental Factor: disease
attributes.value: Spinocerebellar ataxia type 1