title: Kangaroo SIP SG-166-98-2 12C plus hydrogen 'Light'
design: Total RNA extracted from kangaroo foregut samples incubated in the presence of unlabelled (12C) CO2/bicarbonate was recovered from the fraction of CsTFA gradients corresponding to unlabelled 'light' RNA and used as RTPCR template to produce bacterial 16S amplicons. These amplicons were sequenced using 454 technology (GS-FLX titanium).