title: 16S rRNA of mus musculus: isolated and NASH cecum content
design: Total bacterial genomic DNA in cecum content was extracted as described. The V1-V3 region of 16S rRNA genes was amplified and sequenced using the Illumina MiSeq platform (Illumina, San Diego, CA, USA) in Annoroad Gene Technology Co., Ltd (Beijing, China). The resulting 16S rRNA sequences were mainly analyzed based on QiiME2 workflows.