title: NextSeq 500 sequencing; RNA-seq of human mesenchymal stem cells inducibly overexpressing human ataxin-1(Q82)
design: RNA-seq of human mesenchymal stem cells inducibly overexpressing human ataxin-1(Q82)
library_construction_protocol: Samples include technical triplicates of Tet-On YFP-ATXN1(Q82) MSCs cultured in the absence (D0) or presence of Dox for 2, 5 or 10 days (D2, D5 or D10, respectively) and technical replicates of a Venus-tagged MSC line, stably producing a fluorescent variant of YFP.  Total RNA was extracted from samples using the AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN, Hilden, Germany) according to manufacture's protocol.  mRNA was enriched from 250ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Sequencing libaries were prepared from mRNA using SENSE Total RNA-seq Library Prep Kit (Lexogen, Austria), according to manufacture's protocol.
attributes.tag: Experimental Factor: disease
attributes.value: Spinocerebellar ataxia type 1
attributes.tag: Experimental Factor: time
attributes.value: begining of Dox induction