title: GSM2831016: D-CMSC_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from 14 independent human cell lines (7 ND- and 7-D-) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. For next generation sequencing (NGS) RNA was isolated from 14 independent human cell lines (7 ND- and 7-D-) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and libraries integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). 1 µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry.
attributes.tag: GEO Accession
attributes.value: GSM2831016