title: RNA-Seq. S. mediterranea Anterior sagittal Wound site / blastema 48 hour injury
design: "Libraries were prepared as previously described (Engreitz et al., 2014; Schwartz et al., 2014). Briefly, RNA was extracted with TRIzol and following polyA selection (Dynabeads) RNA was fragmented. Then, RNA oligo, corresponding to truncated Illumina primer, was ligated to the 3' end of the fragmented RNA and reverse transcription was performed with complementary sequence. Following RNA digestion, a second oligo was ligated to the cDNA. Finally, libraries were enriched and barcoded by 9-12 cycles of PCR with complete Illumina I7 and I5 oligos."