title: GSM1405841: IL4-treated BMDM_1; Mus musculus; RNA-Seq
library_construction_protocol: Untreated and IL-4-treated BMDMs were lysed in Qiazol and total RNA extracted. The average yield was 20-50 µg of total RNA for each sample. RNA was DNaseI treated (New England Biolabs) and concentrated using RNAclean XP (Beckman Coulter). RNA was then depleted of rRNA using the Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies) followed by concentration using RNA Clean & Concentrator -5 columns (Zymo Research). Illumina sequencing libraries were prepared according to the TruSeq RNA Sample Preparation Guide (Revision A), starting at the RNA fragmentation step. RNA fragmentation with “Elute, Prime, Fragment Mix” was performed for 4 minutes at 94ºC
attributes.tag: GEO Accession
attributes.value: GSM1405841