title: ddRADseq of Centaurea genomic DNA: PstI / MseI
design: Double-digest RADseq using PstI on the forward (R1) end and MseI on the reverse (R2). Barcodes of 6bp length were incorporated into the adapters on both ends and were removed during de-multiplexing of the samples. Fragments of 350-650 bp were size selected with a Pippin Prep. Sequencing was 100bp paired-end Illumina HiSeq 2000.