title: Tumor transcriptome
design: RNA was isolated from the MC-38 tumour cell line by use of Trizol. SureSelect Mouse All Exon Kit was used for capture of the coding sequences. TruSeq Stranded Total RNA sample preparation kit was used to generate RNAseq libraries. Paired-end, 100bp reads were generated and mapping of sequences was done against the mm10 reference genome with BWA (Li H., Durbin R. 2009 Bioinformatics). This data was used to determine the transcriptome and expressed mutations in the MC-38 tumour cell line.