title: 16S DNA Community amplicon of Oceanodroma leucorhoa :  surface burrow soil
design: Bacterial 16S rRNA genes PCR amplified w/ primers for V4 hypervariable region (Kozich et al., 2013). PCR products analyzed on a 1% agarose gel w/ EtBr. DNA libraries were normalized w/ SequalPrep Normalization Plate Kit, 96-well (Thermo Fisher Scientific, Waltham, MA, A1051001), samples pooled and quantified w/ Kapa Biosystems qPCR kit (Kapa Biosystems, Inc., Wilmington, MA, KK4824) then normalized.