title: RNA-Seq of Drosophila nasuta: adult ovaries
design: We dissected ovaries from 5 days old 60 virgin females. Total RNA was extracted using ZR-DuetTM RNA MiniPrepPlus kit from Zymo Research; quantity and quality checked by Qubit and Bioanalyzer. cDNA libraries were prepared using NEBNext UltraTM RNA Library Prep kit for Illumina and validated using Agilent DNA HS and Qubit DNA BR. QC passed libraries were barcoded and sequenced platform for 2x101bp read length.