title: WGS of H. saguini 15-1458 (F3)
design: DNA was isolated using the High Pure PCR template preparation kit (Roche Molecular Biochemicals, Indianapolis, IN).  DNA was then prepared into libraries using the SMRTbell Template Prep Kit 1.0 and the DNA/Polymerase Binding Kit P6 v2, following the manufacturers instructions (Pacific Biosciences, Menlo Park, CA).  DNA libraries were sequenced on a single SMRT cell per genome using a Pacific Biosciences (PacBio) RSII sequencer at the University of Massachusetts Deep Sequencing Core Facility