title: Clostridium thermocellum LQRI genome sequencing
design: genomic DNA underwent Pacific Biosciences standard template preparation and sequencing protocols. Starting material was 5µg of gDNA in conjunction with the low input library preparation protocol. Library preparation selected > 10 kb fragments were selected for LQRI using Covaris g-Tubes and AMPure PB bead-based size selection. Version P6 sequencing polymerase was used. SMRTbell template libraries were sequenced on a Pacific Biosciences RSII sequencer with sequencing version C4 chemistry.