title: GSM3317468: DN3b population, replicate 5; Mus musculus; RNA-Seq
library_construction_protocol: Double-negative developing thymocytes from the DN3 subpopulation were isolated by flow cytometry, gating for CD4-, CD8-, CD3-, B220-, CD25high, CD44low, CD117low expression. DN3 cells were separated in 3 groups based on their CD27 and CD28 expression (DN3a CD27low CD28low, DN3a CD27high CD28low, and DN3b CD27high CD28high) that were pooled for bulk RNA-sequencing Total RNA was isolated from 100,000-200,000 cells with an Ambion RNAqueous Micro Kit (Invitrogen). RNA quality was assessed with a Fragment Analyzer (Advanced Analytical). 50 ng of RNA were used for library preparation (Illumina Truseq stranded kit)
attributes.tag: GEO Accession
attributes.value: GSM3317468