title: GSM4222791: Trophozoites exposed to H202 replicate 2; Entamoeba histolytica; RNA-Seq
library_construction_protocol: Total RNA was extracted from control trophozoites, trophozoites pre-incubated with LB or KB and then exposed (or not) to H2O2(in three biological replicates) using the TRI reagent kit, according to the manufacturer's instructions (Sigma-Aldrich). Libraries were built using a Truseq mRNA-Seq Library Preparation Kit (Illumina), according to the manufacturer's recommendations. Quality control was performed on an Agilent Bioanalyzer. Sequencing was performed on a HiSeq 2500 system (Illumina) and produced 65-base single-end reads Libraries were constructed using Truseq mRNA-Seq Library Preparation Kit (Illumina) following manufacturer's recommendations and were quality controlled using Agilent Bioanalyzer.  Sequencing was performed on a HiSeq 2500 (Illumina) to obtain 65 base single-end reads.
attributes.tag: GEO Accession
attributes.value: GSM4222791