title: GSM3991536: Thoracic Aorta, Tsc1−/− (iii); Mus musculus; RNA-Seq
library_construction_protocol: Thoracic aortas were rapidly excised, flash frozen in liquid nitrogen, crushed tissue was immersed in RLT lysis buffer (Qiagen) and vigorously vortexed, total RNA was isolated using a RNeasy Mini Kit and DNase Digestion Set (Qiagen) according to the manufacturer's protocol, and 500 ng of intact total RNA (RIN>7) was used for polyA selection and construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3991536