title: Tn-seq of Escherichia coli: in vitro growth in urine and in vitro growth in urine
design: INSeq samples were PCR amplified from Escherichia coli strain CFT073 genomic DNA. Input sequences were generated from one pool containing near to 500 transposon mutant bacteria  and output samples were generated from bacteria recovered from murine bladders at 16 h post innoculation and urine samples. Method described in Goodman, A. L. et al. 2011. Nature Protocols.