title: GSM1621328: S. cerevisiae lin3a; Saccharomyces cerevisiae; OTHER
library_construction_protocol: 5 million cells were washed twice in Sorbitol buffer (1.4 M Sorbitol, 40 mM HEPES-KOH pH 7.5, 0.5 mM MgCl2) before being incubated for 30 min at 30 degrees and shaking at 300 rpm with 0.5 mg/mL 100T zymolyase.  The cells were then washed twice in Sorbitol Buffer before being incubated with 2.5 ml of Nextera Transposase in 47.5 ml of 1x TD buffer at 37 degrees for 30 minutes. Sequencing libraries were constructed using a modified version of the Illumina Nextera DNA Sample prep kit, as per Buenrostro et al. (2013).
attributes.tag: GEO Accession
attributes.value: GSM1621328