title: PROK DNA based amplicon 16S rRNA gene of a Dry HYP sample at day 150
design: DNA samples extracted with MoBio PowerSoil. Amplification of Hypervariable V4 region of prokaryotic 16SrRNA gene. 515F-806R primer set.  Dual-indexed Illumina compatible library in a single PCR step. Primary PCR with fused CS primersat their 5' ends. PCR products were normalized using Invitrogen SequalPrep DNA normalization plates.