title: RADtag Gambusia holbrooki: Sample EP64
design: Sequencing adaptors and individual barcodes were ligated to fragments of total genomic DNA obtained by digestion using SbfI restriction enzyme (enzyme target: 5'…CCTGCA(GG)… 3', giving cohesive extremes). The resulting fragments were sequenced from the restriction sites. Individually barcoded RAD samples were jointly sequenced on the Illumina HiSeq2000 platform with single-end 100-bp chemistry. 