title: NextSeq 500 sequencing; High-throughput RNA sequencing of six ant transcriptomes
design: High-throughput RNA sequencing of six ant transcriptomes
library_construction_protocol: Adult worker Lasius niger, Lasius neglectus, Lasius flavus, Myrmica scabrinodis and Formica fusca ants and adult queen Lasius flavus ants were collected from individual nests in Cambridge, UK. Total RNA was extracted from 10-20 adult ants (or 5 queens) using Trizol (Invitrogen) following the manufacturer's instructions. RNA was treated with DNase (Promega, RQ1 RNase-free DNase). Ribosomal RNA was depleted using the Ribo-Zero kit (Illumina), and remaining RNA subjected to alkaline hydrolysis, followed by acrilamide gel purification of RNA bands within the size range 35-50 nt for L. niger and 70-85 for the other species. All library amplicons were constructed using a small RNA cloning strategy (Cook et al., 2013, PMID 24260463; Guo et al., 2010, PMID 20703300).
attributes.tag: Experimental Factor: organism
attributes.value: Lasius flavus