title: 16S library of the picoplankton fraction from surface seawater collected at the Chubut River Estuary (seawater section)
design: Prefiltered surface (~1m depht) seawater was successively filtered through membrane filters of decreasing pore sizes (20, 10, 5 and 0.22 µm) to separate the different cell size fractions. The picoplankton was therefore immobilized onto the 0.22 µm membranes. The picoplankton DNA was extracted from the 0.22 µm filters and 16S sequences were amplified using universal primers 27F and 1492R and pyrosequenced with barcode-tagged 27F primer.