title: GSM4674903: E16.5_KO2_scRNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: Normal or Grhl3 -/-  mouse skin were isolated, made into a single cell suspension, and then processed as per 10X Genomics 3' scRNA-Seq v2 protocol. Cells were washed in PBS with 0.04% BSA and resuspended at a concentration of approximately 1000 cells/µl. Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell Kits User Guide Quantification of cDNA libraries was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA chips (Agilent. 5067-4626). Quantification of library construction was performed using KAPA qPCR (Kapa Biosystems KK4824)
attributes.tag: GEO Accession
attributes.value: GSM4674903