title: m6A-seq of HEK293-TIA1 cells: total RNA, 500 µM AS
design: Total RNA from approximately 45 million HEK 293-TIA1 cells stressed with 500 µM AS was first isolated using Trizol reagent followed by fragmentation using freshly prepared RNA fragmentation buffer. 5 _g fragmented RNA was saved for RNA-seq as input control for m6A-seq.