title: iPSC RNA: Sample 17d0cf06-165a-4603-85c9-e7757d53094d
design: Total RNA was extracted from 222 iPSC lines using AllPrep DNA/RNA Mini Kit (QIAGEN) following the manufacturer's protocol. RNA quality was assessed based on RNA integrity number (RIN) using an Agilent Bioanalyzer. Any samples with RIN less than 7.5 were re-isolated. Libraries were prepared using the Illumina TruSeq stranded mRNA kit and sequenced using an Illumina HiSeq2500 (∼11 samples per lane). Samples were sequenced to an average of ∼22 million read pairs. Biological replicates were sequenced for some lines.
attributes.tag: alignment_software
attributes.value: STAR