title: 16S rDNA amplicon seq of bacteria: surrounding soil
design: Total metagenomic DNA was extracted from surrounding soil of every biological replicate at soybean vegetative stage by using the PowerSoil DNA Isolation Kit, and examined by using 1% agarose gel electrophoresis before the DNA samples were stored at ?20¡ãC in a freezer.Then an improved dual-index high-throughput sequencing approach with paired-end 250 nt was used to create the sequencing library of 16S rRNA gene (16S rDNA) V4 region.