title: Gemome squencebacteria
design: Electrophoresis was performed on 1 sample of RM1 total DNA. A library was constructed using the tested samples. The Illumina-compatible NEB library was used to complete the second-generation genome building of a bacterial DNA sample, and a small fragment library of 350 bp was constructed. The fragment distribution was detected using Aglient 2100, and the library concentration was determined using qPCR. After all the samples were tested, the library was sequenced using Illumina NextSeq500.