title: GSM1289100: Crypt_duodenum_2; Mus musculus; RNA-Seq
library_construction_protocol: Freshly isolated villi, crypts or organoids (cultured for 12 weeks) were used for mRNA sequencing. Total RNA was isolated using Trizol LS reagent (Invitrogen). Total RNA was purified using the Poly(A)Purist MAG Kit (Life Technologies) and subsequently re-purified using the mRNA-ONLY Eukaryotic mRNA Isolation Kit (Epicentre) to obtain full length mRNA. Sequencing libraries were constructed using the SOLiD® Total RNA-Seq Kit (Life technologies) and sequenced on a SOLiD 4 sequencer to produce 50-bp long reads.
attributes.tag: GEO Accession
attributes.value: GSM1289100