title: Interleukin-21 contributes to fatal inflammatory disease in the absence of FoxP3+ T regulatory cells
design: RNA from MACS purified CD4+ T-Cells was extracted and DNase treated using the RNeasy kit (Qiagen) according to manufacturers instruction. RNA integrity (RIN) was assessed as >9 on the Bioanalyzer RNA nano chip (Agilent). Strand-specific RNA sequencing libraries of Poly-A RNA from 500ng total RNA were generated using the SureSelect Strand-Specific RNALibrary Prep for Illumina Multiplexed Sequencing (Agilent). Libraries were sequenced (100bp, paired-end) on the Illumina 2500 platform at the centre for clinical genomics (Garvan Insititue) and fastq files were analyzed.