title: Resource use of benthic biofilms along an altitudinal stream gradient
design: Linking the identity of benthic biofilm taxa (16S amplicons) with the use of different resources (Algae versus Picea needle extract) along an altitudinal stream gradient. Primers used for amplification of the 16S rRNA gene were the bacterial 341F 5’- CCTACGGGNGGCWGCAG -3’ and 805R 5’- GACTACHVGGGTATCTAATCC -3’, containing the 454 Titanium A and B adaptors, respectively. For each sample, two different sample-specific barcodes contained in the forward primer were employed.