title: The effects of knocking down microRNA-related proteins on endogenous microRNA expression.-Xrn2 knock down
design: "RNAi Protocol  Seed cells at 3x106 cells/ml in 6-well plates. Incubate for one hour at 26oC. Centrifuge plate at 1000RPM for 1min. Remove serum-rich media and add 2mls of aliquoted serum-free media with 20ug of dsRNA and pipette up and down to mix. Incubate at 26oC for 1hr. Add 200ul of FBS and return to 26oC for 3 days.  Illumina Sequencing Total RNA was extracted from D. melanogaster S2 cells (Caroline Milner, University of Manchester) using the Ambion miRvana kit according to the manufacturer's instructions. Small RNA libraries were prepared using the Illumina TruSeq ? Small RNA Kit and Illumina sequencing was performed by either GATC or the University of Manchester Genomics Facility using a HiSeq 2000 machine."