title: GSM1289022: Cont0; Homo sapiens; MBD-Seq
library_construction_protocol: After FACS selection, genomic DNA from infected and uninfected cells was purified using a conventional proteinase K/organic extraction method. Methylated DNA was captured using the MethylCap kit (Diagenode AF-100-0048), Belgium), followed by high throughput sequencing  at Nxt-Dx (Belgium). The methylated fragments were eluted in a single-step using High-Elution buffer. End repair, <A> base addition and adaptor ligation steps were performed following ‘multiplexed paired end ChIP protocol’ (Illumina, San Diego, California, USA) and Apollo 324™ automated DNA library preparation platform system IntegenX™ (CA, USA). Libraries were amplified by PCR. Fragments of 300 bp +/- 50 bp from the amplified libraries were excised after separating on a 2% agarose gel (Low Range Ultra agarose Biorad 161-3107). DNA from excised gel pieces was eluted using Qiagen Gel Extraction Kit (Qiagen). Purified libraries were quality checked for concentration with smear analysis using Agilent 2100 (Agilent Technologies, Santa Clara, California, USA).deep sequencing using HiSeq2000 (Illumina) to generate 50 bp-long paired-end reads.
attributes.tag: GEO Accession
attributes.value: GSM1289022