title: biological replicate of Malpighian tubules tissue in Chrysomela populi
design: We extracted the total RNA by using the RNAqueous Micro Kit including DNase treatment(Ambion, Life Technologies) from Malpighian tubules tissues from five Chrysomela populi larvae(third-instar). The RNA integrity was validated by using RNA 6000 Nano labchips on a Bioanalyzer 2100 (Agilent Technologies). Library preparation for sequencing was carried out with 5 *g of total RNA by using TruSeq™ RNA Sample Prep Kit (illumina). For the RNA sequencing we ran illumina next-generation sequencing technique (RNA-seq), multiplexed two or three samples in one lane.