title: RNA-Seq of T. ouvatus fed by PF:juvenile liver
design: After  total  RNA  was  extracted,  eukaryotic  mRNA  was  enriched  by  Oligo(dT)  beads,  while  prokaryotic mRNA  was  enriched  by  removing  rRNA  by  Ribo-Zero TM Magnetic  Kit  (Epicentre).  Then  the  enriched mRNA  was  fragmented  into  short  fragments  using  fragmentation  buffer  and  reverse  transcripted  into cDNA with random primers.  Second-strand cDNA was  synthesized by DNA polymerase I,  RNase H, dNTP and  buffer .  Then  the  cDNA  fragments  were  purified  with  QiaQuick  PCR  extraction  kit,  end  repaired, poly(A)  added, and ligated to Illumina sequencing adapters. The ligation products were size  selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeqTM4000 by Gene Denovo Biotechnology Co. (Guangzhou, China).