title: GSM3855795: Bulk_Human_Donor001_Treg; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq was performed as described previously by Seumois et al 2016 and Ganesan et al 2017 using smart-seq2 method  with 0.5ng of total RNA. Total RNA was purified using a miRNAeasy kit (Qiagen) and quantified as described previously (Seumois et al.Am J Clin Exp Immunol. 2012; PMID:23304658). Barcoded Illumina sequencing libraries were constructed as per the protocol described by illumina (Nextera XT library preparation kit, Illumina). DNA sequencing - Libraries were sequenced on the HiSeq2500 Illumina platform to obtain 50-bp single end reads and on the NovaSeq6000 to obtain 50-bp paired end reads (barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina)).
attributes.tag: GEO Accession
attributes.value: GSM3855795