title: GSM3543811: MDA_48h_untreated-2; Homo sapiens; ssRNA-seq
library_construction_protocol: The treated cells were harvested, and the cell pellets were snap frozen with liquid nitrogen and stored at−80°C.Total RNA was isolated with PureLink™ RNA Mini Kit (Thermo Fisher Scientific) according to the manufacturer's protocol. After quantified using a NanoDrop Spectrophotometer ND-1000 (Thermo Fisher Scientific), the quality of the total RNA was verified on a Bioanalyzer by Cancer Genome Facility (SA, Australia) ensuring all samples had RINs >7.0. For both cell lines, the sequencing was performed in Ramaciotti Centrefor Genomics (NSW, Australia). The sample preparation for each cell line was TruSeq Stranded mRNA-seq with dual indexed, on the NextSeq500 v2 platform. The parameter was 75bp paired-end High Output. Total RNA was isolated with PureLink™ RNA Mini Kit (Thermo Fisher Scientific) according to the manufacturer's protocol (Rev: 21 May 2012).  Sample preparation were employing TruSeq Stranded mRNA Library Prep Kit (Illumina) with 1μg input total RNA.
attributes.tag: GEO Accession
attributes.value: GSM3543811