title: GSM1293745: 145; Homo sapiens; RNA-Seq
library_construction_protocol: Add 200ul chloroform plus 1mL TRIZOL, and vortex for 15 seconds, and  then centrifuge samples at 12,000g for 15 minutes at 2-8 C. Transfer the aqueous phase to a fresh tube. Add 500ul isopropanol plus 1mL TRIZOL to the this new tube, vortex and centrifuge samples at 12,000g for 10 minutes at 2-8 C again. Following centrifugation, remove the supernatant. Wash RNA pellet with 70% alcohol plus 1ml TRIZOL, vortex, and Centrifuge samples again. Remove supernatant and dry the pellet, then redissolve it in 100ul DEPC water. After Dnase Treatment, RNA was purified using Rneasy Column Purification based on Qiagen's Rneasy Protocol. The protocol was based on TruSeq RNA Sample Preparation Kits v2
attributes.tag: GEO Accession
attributes.value: GSM1293745