title: Transcriptomic alterations in the mitochondrial 3-hydroxyacyl-ACP dehydratase-deficient mutant mthd-rnai-1 in ambient air and in the 1% CO2 atmosphere.
design: Experiment design: The mthd-rnai-1 mutants were grown on Murashige and Skoog (MS) agar medium for 16 days at a temperature of 22°C, at a continuous illumination of 100 µmol of photons m-2 s-1, and in ambient air. Three samples were collected for each combination of genotype and atmospheric condition. All MS plates were randomly placed in the growth chamber. Total RNA was extracted from 2 g fresh aerial organs using TRIzol (Life Technologies, Carlsbad, CA), and further purified using the QIAGEN RNeasy Mini Kit (QIAGEN, Valencia, CA) following the DNase I (Life Technologies, Carlsbad, CA) treatment to remove any DNA contamination. The 200 bp short-insert library was constructed. Transcriptome sequencing was performed at BGI Americas (http://bgiamericas.com) using an Illumina HiSeq 2000 system and V3 Reagent (91 pair end sequencing).