title: GSM1403297: mRNA_seq_WT; Mus musculus; RNA-Seq
library_construction_protocol: For mRNA-sequencing, we obtained total RNAs from each sample and then poly(A) mRNA isolation from total RNA (5 µg) and fragmentation were performed using the Illumina Truseq RNA Sample Prep Kit Ver. 2 with poly-T oligo-attached magnetic beads, according to the manufacturer’s instructions.  Reverse transcription of RNA fragments was performed using Superscript II reverse transcriptase (Life Technologies). For ChIP-sequencing, extracts containing DNA fragments with an average size of 400bp were immmunoprecipitated by using antibodies against GFP (control) or Pontin
attributes.tag: GEO Accession
attributes.value: GSM1403297