title: GSM4222840: wt E9.5 embryo miRNA, replicate 2; Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol according to the manufacturer's instructions (Invitrogen) miRNA libraries were prepared as described in Belair et al. 2019. In brief, miRNAs were purified from total RNA using the miRNeasy kit (Qiagen). miRNAs were then ligated to 3' adaptors with NNNN at each 5' end to reduce ligation bias and a unique barcode for each sample. Each 3' ligated sample was then size-selected for miRNAs and pooled. The pool was ligated to a common 5' adapter, reverse-transcribed, amplified for 14 cycles and single-end sequenced on a Hi-seq4000.
attributes.tag: GEO Accession
attributes.value: GSM4222840