title: GSM2409728: BY_MINIMAL_PLUS_3; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: RNA used for library preparation was treated with Turbo DNase prior to any downstream manipulations.  Total RNA was not depleted of rRNA nor fragmented. Total RNA was either incubated with ADP-ribosylcyclase (or mock-treated as a control) in the presence of the alkyne 4-pentynol.  RNA was re-extracted with phenol:chloroform and ethanol precipitated.  This RNA was then biotinylated using copper sulfate and biotin-peg3-azide, and next extracted and precipitated as before.  Next this RNA-biotin was coupled to magnetic streptavidin beads. Library preparation was initiated by first strand cDNA synthesis on biotinylated RNA that was coupled to streptavidin beads.  Downstream library preparation was carried out using adaptors/primers described in Zhang et al, 2012 using standard Illumina protocols.  
attributes.tag: GEO Accession
attributes.value: GSM2409728