title: GSM3317501: Venus(+)Ly6Chi Monocyte; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow cells were prepared from mice 48 h after systemic LPS injection and sorted using a cell sorter. Sorted cells were lysed in TRIzol LS reagent (Thermo Fisher Scientific) and total RNA was extracted using RNeasy Mini or Micro Kit (QIAGEN), a FavorPrep Total RNA Extraction Column (Favorgen, Taiwan) according to the manufacturer's protocol. DNA library preparation for RNA-Seq analysis using a SureSelect Strand-Specific RNA Library Prep Kit for Illumina Multiplexed Sequencing (Agilent Technologies).
attributes.tag: GEO Accession
attributes.value: GSM3317501