title: Microbiome and immune responses
design: DNA was extracted using the MO BIO PowerSoil kit. The V1-V2 variable regions of the bacterial 16S rRNA gene were amplified using barcoded primer sequences 27F (AGAGTTTGATCCTGGCTCAG) and 338R (TGCTGCCTCCCGTAGGAGT). Purified products were pooled in equal amounts and sequenced on the Illumina MiSeq. The paired-end sequencing protocol was used, yielding 250bp reads in the forward and reverse directions.