title: GSM3386112: Caki-1 cell line si-HOIL-1#1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent(Life Technologies). Illumina NEB Ultra directional RNA library Prep Kit was used with 1 ug of total RNA for the construction sequencing libraries. Total RNA was extracted and ribosomal RNA was removed using the Ribo-Zero™ kit (Epicentre, Madison, WI, USA).Fragmented RNA were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit®2.0(Life Technologies, USA). The libraries were paired-end sequenced at Guangzhou RiboBio Co., Ltd. using IlluminaHiSeq 3000 platform.
attributes.tag: GEO Accession
attributes.value: GSM3386112