title: 16S-Miseq sequencing from pooled guts of A.echinatior lab colony Ae322 reared on sucrose -replicate 23E1
design: "16S rRNA gene libraries were constructed using different indexed primers to amplify the V34, V4, and V45 regions from genomic DNA. The purified amplicons were pooled in equimolar concentrations, the final concentration of the library was determined and was sent for sequencing."