title: NextSeq 500 paired end sequencing; Gene expression profiles during Phallusia mammillata embryogenesis
design: Gene expression profiles during Phallusia mammillata embryogenesis
library_construction_protocol: Adult Phallusia mammillata animals were obtained from the Roscoff Marine Station (France). Staged embryos were obtained after in vitro fertilization of eggs. RNA was extracted from staged embryos using the MirVana kit (Ambion) following the manufacturer's instructions.  Barcoded mRNA libraries were prepared using TruSeq RNA Sample Preparation (Illumina) following the manufacturer's instructions.
attributes.tag: Experimental Factor: developmental stage
attributes.value: Early Gastrula St11