title: Whole Genome Sequencing of Dicerorhinus sumatrensis
design: One paired-end library and eight mate pair libraries were prepared from the specimen's purified genomic DNA and sequenced. Paired end library was prepared using Illumina TruSeq PCR-free method from genomic DNA according to manufacturer's instructions. Average insert size for this library was 462 bp. These libraries were sequenced in three separate 2x250 bp paired end HiSeq 1500 Rapid Runs. Gel-free and gel-plus mate pair libraries were prepared using the Nextera Mate Pair library kit according to manufacturer's instructions.  Gel-plus libraries were prepared from 12 DNA fragments in three size ranges: 4-6kb, 6-9kb and 9-12kb. Adaptor enrichment (library amplification) was 10 cycles of PCR for gel-free libraries and 15 cycles of PCR for gel-plus libraries. Two replicates were generated for each gel-free and gelplus mate pair library, resulting in 8 libraries in total.  Average library insert sizes for gel-free and gel-plus libraries ranged from 345 to 515 bp and from 240 to 363 bp, respectively.  Mate pair libraries were sequenced in a 2 x 150 bp paired-end Rapid Run mode.