title: Nanopore sequencing of E. coli CKP2
design: DNA from the samples were end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA,USA), cleaned up with 1x AmPure beads (Beckmann Coulter, USA). Native barcode ligation wasperformed with NEB blunt/ TA ligase (New England Biolabs, MA, USA) and cleaned with 0.5xAmPure beads. Qubit quantified barcode ligated DNA samples were pooled at equimolar concentration to attain 1 g pooled sample. Adapter ligation (BAM) was performed for 15 minutes using NEBnext Quick Ligation Module (New England Biolabs, MA, USA). Library mix was cleaned up using 0.4X AmPure beads (Beckmann Coulter, USA) and finally sequencing library was eluted in 15 l of elution buffer and usedfor sequencing.