title: GSM4221855: M9 3866 3; Escherichia coli; RNA-Seq
library_construction_protocol: 10 mL RNAprotect Bacteria Reagent was immediately added and mixed with the drugged cultures and pelleted at 5,000XG for 10 minutes.  The supernatant was discarded and the pellets were flash frozen.  For RNA extraction, pellets of E. coli K12 were re-suspended in 100 µL of 1 mg/mL lysozyme and incubated at 37°C for 10 minutes with 5 seconds vortex every 2 minutes. Subsequent steps were carried out using the RNeasy extraction kit (Qiagen) according to protocol. RNA was eluted in 40-50 µL of supplied elution buffer. Residual genomic DNA contamination was depleted using RNA Clean and Concentrator kit (Zymo Research) following the manufacture's protocol. Samples were eluted in 50 µL of DNase/Rnase-Free water. Quality of RNA samples were measured using an Agilent 2100 Bioanalyzer and RIN values were all > 7.0. ScriptSeq™ v2 RNA-Seq Library Preparation Kit, standard protocol.
attributes.tag: GEO Accession
attributes.value: GSM4221855