title: Illumina HiSeq 2500 paired end sequencing; The faint sausage gene encodes a subunit of the spliceosome-activating Prp19 complex and is required for tracheal branching morphogenesis in Drosophila
design: The faint sausage gene encodes a subunit of the spliceosome-activating Prp19 complex and is required for tracheal branching morphogenesis in Drosophila
library_construction_protocol: Drosophila stocks were obtained from the Bloomington Stock center: btl724 (Ghabrial and Krasnow, 2006), fas1  genomic fas+, UAS-fas-myc, fasfand1, fasfand2 (Guilgur et al., 2014), Mi{MIC}CG17716MI02461 (Venken et al., 2011), btl-Gal4 (Shiga et al., 1996), btl-mCherry-nls (gift from Emmanuel Caussinus) UAS-λBtl (Lee et al., 1996), AbdB-Gal4 (de Navas et al., 2006). The fasH124 and fasP218 mutations were isolated in a mutagenesis screen, in which a btl-Gal4 UAS-GFP UAS-Verm-mRFP chromosome (Förster et al., 2010) was mutagenized with ethyl methanesulfonate (EMS). Total RNA was isolated from 12-13h old fasP218 btl-Gal4 UAS-GFP UAS-Verm-mRFP mutant embryos and from control embryos carrying the non-mutagenized parental btl-Gal4 UAS-GFP UAS-Verm-mRFP chromosome using TRIzol Reagent (ThermoFischer) according to the supplier´s protocol. cDNA libraries were generated using the standard Illumina RNASeq protocol.
attributes.tag: Experimental Factor: fas P218 mutation
attributes.value: genotype