title: GSM1288759: med27 RNAi central brain pupal neuroblasts 1 (Tech. Rep. 2); Drosophila melanogaster; RNA-Seq
library_construction_protocol: Central brain neuroblasts (large cells, high GFP expression) were FACS sorted from wild type (TID:60100) or med27 RNAi and RNA was isolated using Trizol reagent. Total RNA(~1µg) was enriched for polyA mRNA, fragmented and first strand and second strand (dUTP, instead dTTP was used) was generated. Library preparation was done using a modified protocol from Illumina with NEBNext DNA sample Prep Reagent kits (NEB), and UGDase treatment was done to confer strand specificity.
attributes.tag: GEO Accession
attributes.value: GSM1288759