title: RNA-seq - Chronic wound - 2
design: Total RNA was prepared with RNA-Bee (Tel-Test). Ribosomal RNA was depleted with the Ribo-Zero rRNA Removal Kit (Bacteria) and the Ribo-Zero Gold Kit (Human/Mouse/Rat) (Epicentre). Remaining RNA was fragmented with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Libraries were prepared with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs). Finished libraries were size-selected for fragments ~140-300bp.