title: Illumina HiSeq 2000 sequencing; Modified iCLIP sequencing of HEK293 cells with antibody PTBP1
design: Modified iCLIP sequencing of HEK293 cells with antibody PTBP1
library_construction_protocol: HEK293 cells were crosslinked with 0.15mJ/cm2 254nm UV light. Following the iCLIP protocol the retrieval of protein-bound RNAs and construction of Illumina compatible libraries was done as described in Huppertz et al, 2014. The 3 prime adapter sequence is AGATCGGAAG
attributes.tag: Experimental Factor: replicate 2
attributes.value: individual
attributes.tag: Experimental Factor: antibody PTBP1
attributes.value: immunoprecipitate