title: GSM3317165: GCEPB-_Replicate3; Mus musculus; RNA-Seq
library_construction_protocol: Cell populations were directly FACS-sorted into PicoPure RNA isolation kit (Arcturus) and immediately frozen on dry ice to increase RNA yield. All RNA preparations were treated with DNAse(Qiagen). Whole transcriptome amplification was performed using the SMARTer Ultra Low Input RNA Sequencing-HV Kit (Clontech), which utilizes modified oligo(dT) primers. cDNA libraries were generated with Nextera transposase (Illumina) according to the manufacturer's protocol. The samples were analyzed on an Agilent Bioanalyzer. High quality libraries were sequenced as paired-end 100bp reads on a HiSeq2000/2500 (Illumina) at Columbia Genome Center.
attributes.tag: GEO Accession
attributes.value: GSM3317165