title: Whole genome sequencing of independently derived 5FC resistant colonies of Cryptococcus deuterogattii
design: Single colonies were expanded in 50 mL YPD liquid cultures with 5FC. Cultures were concentrated by centrifugation and lyophilized. DNA was then isolated using a CTAB DNA prep, and the DNA was treated with RNAase. DNA was re-extracted using phenol-chloroform. Paired end libraries were then prepared using a Kapa hyper prep and sequenced by the Duke University Sequencing Core using the NovaSeq platform.