title: GSM4064967: mRNA sequencing data from T. microellipsoides white culture 3; Torulaspora microellipsoides; RNA-Seq
library_construction_protocol: Genomic DNA was purified using a standard phenol-chloroform based extraction. DNA was then treated with RNaseA for 1 hour at 37⁰C, and re-purified with an additional phenol-chroloform extraction. Sequencing libraries were prepared using the Nextera DNA Flex library preparation kit, according to manufacturer's instructions (Illumina). Total RNA was purified using a standard hot acid-phenol chloroform extraction. RNA was then treated with DNaseI for 1 hour, and re-purified with an additional acid-phenol chloroform extraction. Sequencing libraries were prepared using the Nextera DNA Flex library preparation kit, according to manufacturer's instructions (Illumina). Libraries were pooled and loaded onto a NextSeq High Output flow cell, generating paired-end 150 bp reads. RNA was quantified using Qubit fluorometry and 500 ng of each sample used to prepare sequencing libraries were prepared with the TruSeq Stranded mRNA library preparation kit, according to manufacturer's instructions (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4064967