title: 16S V4 rRNA Hirudo verbana : Intaluminal fluid
design: Amplified the V4 hypervariable region of the 16S ribosomal RNA (rRNA) gene using primers designed in (Kozich, 2013). PCR reactions were prepared in triplicate and sequenced using an Ilumina MiSeq with custom sequencing primers added to the reagent cartridge and sequenced 2  250bp