title: gene expression profiles in peripheral blood samples collected from patients with H7N9 infection and healthy people
design: RNA extraction from whole blood was carried out using the PreAnalytiX PAXgene Blood RNA Kit. followed by thermal mRNA fragmentation. Total RNA was first selected using oligo-d(T) probes for poly-A mRNAs and the fragmented RNA was subjected to cDNA synthesis and further converted into double-stranded cDNA. Upun end repairing, the cDNA product was ligated to Illumina Truseq adaptors and size selected using the 2% agarose gel to generate the cDNA libraries. Libraries from the patients samples were used to generate the raw sequence reads by the Illumina HiSeq sequencer