title: GSM2670794: UPF1_3; Homo sapiens; miRNA-Seq
library_construction_protocol: Small RNA-enriched fractions were purified from cell pellets using miRNeasy and RNeasy MinElute Cleanup Kits (Qiagen). cDNA libraries were generated using the TruSeq Small RNA library preparation protocol (Illumina). After addition of the TruSeq 3' adapter (5' adenylated) using truncated T4 RNA ligase II (New England Biolabs) and, subsequently, the 5' adapter using T4 RNA ligase I (New England Biolabs), RNA was reverse-transcribed using a 3' adapter-specific primer. The resulting cDNA was then amplified using 12 cycles of PCR, a universal forward primer and a reverse primer, the latter harboring a bar code. cDNA libraries were purified using the Pippin Prep system (Sage Sicence) and quantified first using an Agilent Bioanalyzer and subsequently using qPCR. Barcoded libraries were mixed at equimolar concentrations and analyzed by single-end 50-nt sequencings using an Illumina HISeq2500v4 sequencer (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2670794