title: GSM2409723: SATB1_input_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were subjected to crosslinking and nuclei were lysed. Nuclei sample was sonicated using a model XL2000 ultrasonic cell disruptor (MICROSON). Sonicated chromatin was incubated overnight at 4 °C with anti-SATB1 antibody (Abcam, ab70001) that was preconjugated with magnetic beads (Dynabeads). Immunoprecipitated DNA was then purified. Libraries were prepared according to NEB's instructions accompanying the NEBNext ChIP-seq Library prep Kit (E6240). The purified library was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM2409723