title: Fungal ITS sequences from sample PBS-off2, soil from outside PBS Mn(II) removal bed (PA, USA)
design: Amplicon pyrosequencing of fungal ITS1-5.8S-ITS2 region with forward primer CTTGGTCATTTAGAGGAAGTAA (ITS1F) and reverse primer TCCTCCGCTTATTGATATGC (ITS4).