title: GSM2482969: Tdrd6+/- 1; Mus musculus; RNA-Seq
library_construction_protocol: Testes from mice 20dpp were removed, hCD4+ cells were isolated via MACS and RNA was harvested from these cells using Trizol (Invitrogen) reagent according to manufacturer's instructions. As a first step of library preparation, genomic DNA was removed from the total RNA with DNase I, followed by rRNA depletion using the Ribo-Zero™ Magnetic Gold Kit from Epicentre (an Illumina company). Afterwards, libraries were prepared by following the protocol for NEBNext® Ultra Directional RNA Library Prep Kit.
attributes.tag: GEO Accession
attributes.value: GSM2482969