title: GSM3854957: Bahd1-WT Adult rep1_rc; Mus musculus; RNA-Seq
library_construction_protocol: Brains were dissected and cut sagitally. Halves of the brain were frozen in RNAlater and further processed for Total RNA extraction, with RNeasy kit (Qiagen) according to the manufacturer's instructions. (Note: the procedureremoves RNA smaller than 200 nucleotides). RNA samples were treated with Turbo DNase-free kit (Invitrogen) to remove residual DNA and were quality checked (Bioanalyser Agilent). Illumina TruSeq RNA Sample Prep Kit (Illumina, #RS-122-9004DOC) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3854957