title: GSM4222676: AJ73_RNA_rep2; Homo sapiens; OTHER
library_construction_protocol: Qiagen RNeasy Plus Mini kit and Qiagen Plasmid Plus Mini kit, mRNA enrichment was done using polyT Dynabeads and  RT was performed by superscript III reverse trascriptase. Reporter-specific libraries were generate by first using reporter-specific primers during RT and PCR. Sequencing libraries were prepared according to Illumina's instructions. A standard flow cell adapters and indexes were added to all DNA and cDNA reactions through an 6 cycles of PCR.The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Nextseq 500/550 v2 75 cycle mid-output kit following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4222676