title: GSM3929520: CITE-seq Met v Naïve HTO; Mus musculus; OTHER
library_construction_protocol: Mice were perfused with PBS and brains extracted. Brains digested with papain-based neural tissue dissocation kit (Miltenyi). Mononuclear cells enriched by 70%/37%/30% percoll gradient centrifugation. For RNA expression (cDNA), we followed 10x genomics single cell 3' v2 assay standard protocol. For ADT and HTO, we followed CITE-seq and Cell hashing protocol as provided here:https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf 10x genomics single cell 3' v2 assay
attributes.tag: GEO Accession
attributes.value: GSM3929520