title: GSM1948717: WE; Xenopus laevis; RNA-Seq
library_construction_protocol: RNA from wild type whole embryos or dorsal lips or ventral lips was isolated with an Absolutely RNA miniprep Kit (Agilent). Libraries were constructed with the Illumina TruSeq RNA Library Preparation Kit V2 according to manufacturer's protocol. Briefly, 1 µg total RNA from stage 10.5 wild type embryos, dorsal lips or ventral lips was used for input RNA. Then mRNA was purified through polyA+ selection. After fragmentation of the mRNA, first strand and second strand synthesis, double strand cDNA (ds cDNA) was obtained. The ds cDNA was then further processed for A-tailing, end repair and ligation of appropriate adaptors. Finally, the ds cDNA with adaptors was amplified by PCR to generate libraries. After measurement of the length and concentration of the libraries by Bioanalyser and Qubit, the libraries were sent for sequencing on Illumina Hi-Seq 2000 using standard methods to generate 100-bp single end reads by the Broad Stem Cell Research Center at UCLA.
attributes.tag: GEO Accession
attributes.value: GSM1948717