title: GSM1290518: Control_naive_A4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRI Reagent (Sigma-Aldrich) according to the suppliers manual. Following DNase1 (life Technologies) treatment for 20 min at 37 °C, RNA was again purified using phenol-chloroform extraction. Library preparation and cluster generation for mRNA sequencing was performed according to Illumina standard protocols using the TruSeq RNA Sample Prep Kit v2 and the TruSeq Paired-End Cluster Generation Kit v3-cBot-HS (for PE mRNA-seq) with subsequent use of the corresponding TruSeq Cluster Generation Kit 3-cBot-HS (for SE mRNA-seq). Libraries were quality controlled and quantified using a Nanodrop 2000 (Thermo Scientific), an Agilent 2100 Bioanalyzer (Agilent Technologies) and Qubit (life Technologies).
attributes.tag: GEO Accession
attributes.value: GSM1290518