title: miRNA-seq of Glossina pallidipes: gravid female whole body
design: Method: Tissue miRNA profiles of gravid females were generated using high throughput sequencing by Illumina Hiseq 2500 in triplicates. The sequences that passed quality check were analyzed using miRDeep2 V2.0.08: the reads were aligned to the Glossina pallidipes genome (build GCA_000688715.1) using Bowtie and followed by statistical analysis using Perseus and Empirical Analysis of Digital Gene Expression Data v3.16.5 (edgeR) package in R