title: GSM1287774: Patient_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using RNAeasy mini kit(Qiagen). Libraries were prepared according to 5500 series SOLiD system instructions accompanying the Library Builder™ . First the RNA was concentrated using RiboMinus (RiboMinus™ Eukaryote System v2, Catalog Numbers A15020,). The rRNA depleted RNA is clean up on columns using the Concentrator module ((Cat. no. K1550-05) ). rRNA depleted RNA is quantified using  Qubit® RNA Assay Kit and Qubit® 2.0 Fluorometer. Then verification of rRNA depletion is done using Agilent RNA 6000 Pico Kit. Further, SOLiD™ Total RNA-Seq Kit (PN 4445374) to convert RNA transcripts expressed in a cell or tissue into a cDNA library for analysis on the Applied Biosystems SOLiD™ Sequencing System. Frist RNA is  fragmented into ~150bp fragments using enzyme RNaseIII afterwards the fragmented RNA is put in the library builder (Pat no. 4463421)were reverse transcription and size selection is done. Barcodes are put during the amplification step.
attributes.tag: GEO Accession
attributes.value: GSM1287774