title: ONT sequencing of Apis mellifera: Adult female
design: DNA from a highland sample was prepared using Qiagen Blood & Tissue column, concentrated with 0.6x Ampure Xp and fragment ends were prepared for ligation using the NebNext Ultra-II kit (20 minutes at room temperature followed by 20 minutes at 65C). DNA was purified using 0.6x Ampure XP, eluted in 5 l water + 20 l PCA adaptors followed by ligation for 10 minutes at room temperature with 25 l Blunt/TA ligase (NEB). PCR and sequencing was done according to the ONT protocol for PCR barcoding.