title: RNA-seq of rice: leaf of heat-stressed samples biorep2
design: Samples were ground in liquid nitrogen using mortar and pestle. An RNeasy Plant Mini Kit (Qiagen) was used to extract total RNA following the manufacturers instructions. The amount of extracted RNA was estimated using the Qubit Fluorometric Quantitation system (Thermo Fisher). An Illumina TruSeq Stranded mRNA Library Prep kit (Illumina) was used according to the manufacturers instructions. The resulting library was run on an Illumina NextSeq 500 machine (Illumina) in the Sainsbury Laboratory at the University of Cambridge.