title: GSM1404206: Siwi bound piRNAs from BmN4; Bombyx mori; RIP-Seq
library_construction_protocol: Siwi and BmAgo3 complexes were immunopurified from BmN4 cells using anti-Siwi and anti-BmAgo3 antibody bound to Dynabeads protein G (Invitrogen). The beads were washed three times with binding buffer [30 mM HEPES (pH 7.4), 150 mM potassium acetate, 5 mM magnesium acetate, 5 mM DTT, 0.1 % NP-40, 2 mg/mL pepstatin, 2 mg/mL leupeptin and 0.5% aprotinin] containing 500mM sodium chloride and twice with binding buffer. Total RNAs were eluted from the beads using phenol–chloroform and precipitated using ethanol. small RNA-seq libraries were prepared using NEBNext Small RNA Library Sample Prep Set (New England BioLabs (NEB), E7330).
attributes.tag: GEO Accession
attributes.value: GSM1404206