title: GSM1287703: H2AZ_K3R3_ESC; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol. Strand specific libraries were prepared with the purified RNA subjected to oligo (dT) selection, fragmentation and first and double strand synthesis with the Illumina Tru-Seq kit using manufacturer’s instructions. DNA fragments above 30bp was purified using SPRI-TE beads according to manufacturer’s instructions. The purified DNA was end repaired and single A bases were added for adaptor ligations. The adaptor-ligated DNA was then subjected to  Pippin purification to select for 300bp DNA fragments. These fragments were enriched and barcoded by PCR for multiplexing. A final Pippin purification was performed to clean up the barcoded RNA-Seq libraries.
attributes.tag: GEO Accession
attributes.value: GSM1287703