title: GSM4182967: CHLA-266 sh-HHIP-AS1#1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using Trizol (#15596-018, Thermo Scientific) 500 ng total RNA was processed using the TruSeq RNA Sample Preparation v2 Kit (low-throughput protocol; Illumina, San Diego, USA) to prepare the barcoded libraries. Libraries were validated and quantified using DNA 1000 and high-sensitivity chips on a Bioanalyzer (Agilent, Böblingen, Germany); 7.5 pM denatured libraries were used as input into cBot (Illumina), followed by deep sequencing using HiSeq 2500 (Illumina) for 101 cycles, with an additional seven cycles for index reading.
attributes.tag: GEO Accession
attributes.value: GSM4182967