title: RNA-seq of Dictyostelium discoideum AX3: single cell
design: log phase cells were grown in tissue culture dishes before being harvested and resuspended at a density of 1x106 cells/ml in HL5 for capture on a Fluidigm C1 flow cell using a medium size chip. This resulted in the capture of 81 individual cells, which were sequenced on an Illumina Hiseq 4000 resulting in 100bp paired-end reads (150bp insert).