title: 16S and ITS rDNA ampilcon sequencing of soil microbiome: rhizosphere Dactylis glomerata
design: DNA extracted from rhizosphere soil of plant phytometers using PowerSoil DNA Isolation Kit (MO BIO Laboratories Inc., Carlsbad, CA, USA). 16S and ITS rDNA amplicon libraries of V4 and ITS2 region performed on the Illumina MiSeq platform using the primers  515f-806r and fITS7-ITS4 for bacteria and fungi, respectively.