title: GSM1337157: HA-Ago3 IP  #rep1; Bombyx mori; RNA-Seq
library_construction_protocol: Nucleic acids were isolated from immunoprecipitated HA-tagged protein complexes and resolved by 15% urea-PAGE. Bands corresponding to piRNAs were excised from the gel and extracted with 400 µl of 0.3M NaCl solution at 25°C overnight. After purification by phenol-chloroform extraction, deep sequencing libraries were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions. Libraries were sequenced on Illumina HiSeq 2000 platform (EMBL Heidelberg Gene core facility).
attributes.tag: GEO Accession
attributes.value: GSM1337157