title: Illumina HiSeq 2000 sequencing; hMeDIP-seq of mouse intestinal stem and differentiated cells
design: hMeDIP-seq of mouse intestinal stem and differentiated cells
library_construction_protocol: Lgr5-EGFP-IRES-CreERt2 mice used to isolate Lgr5+ intestinal stem cell (GFP+ cell sorted by FACS), Villus differentiated cells were collected by gentle scraping Using NEBNext® DNA Library Prep Master Mix Set for Illumina®
attributes.tag: Experimental Factor: differentiated villus cell
attributes.value: cell type
attributes.tag: Experimental Factor: 5-hydroxymethylcytosine
attributes.value: immunoprecipitate