title: GSM1293572: SampleNo_954 Cells_old; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from 400 uL of serum from 11 young (30 yrs) and 11 old (64 yrs) or peripheral blood mononuclear cells (one young 30 yr old and one old 64 yr old ) from HANDLS participants using Trizol LS (Life Technologies) according to manufacturer’s instructions with the addition of a second phenol/chloroform extraction prior to RNA precipitation in order to reduce protein and DNA contamination.  Samples were resuspended in 15 mL RNase-free water and frozen at -80oC until further use. Libraries were prepared for sequencing using the TruSeq small RNA sample prep kit (Illumina) with multiplexing according to manufacturer's instructions
attributes.tag: GEO Accession
attributes.value: GSM1293572