title: GSM1289484: HUVEC0_n1; Homo sapiens; MNase-Seq
library_construction_protocol: HUVECs stimulated with TNFα for 0-30 min were digested (3 min; at 37°C) with 750 units of micrococcal nuclease (MNase; Sigma-Aldrich ). Mononucleosomal DNA was isolated following separation on 1.3% agarose gels using glass beads (Qiagen) and ethanol precipitation. Libraries were generated using the NEBNext DNA library prep Master Mix Kit (NEB) according to the manufacturer's instructions and paired-end (2x 50 bp) sequenced on a HiSeq2000 platform (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM1289484