title: Microbiome of Porites lobata: Parrotfish wounding and feces deposition
design: DNA extraction was performed using DNeasy PowerSoil Kit (Qiagen) according to the manufacturer instructions. Together with AccuStart II PCR ToughMix PCR reagent (Quanta BioSciences, Gaithersburg, Maryland, USA) a two-step Polymerase Chain Reaction (PCR) was performed on the V4 hypervariable region of the 16S rRNA gene using the primer pair 515Fb (5-GTG CCA GCM GCC GCG GTA A-3) and 806Rb (5-GGA CTA CHV GGG TWT CTA AT-3).  Barcoded amplicons were combined in equivolume ratios, and the pools purified using the Agencourt AMPure XP beads. Relative libraries were submitted to the Center for Genome Research and Biocomputing (CGRB) at Oregon State University (OSU) for sequencing on the Illumina MiSeq Platform.