title: GSM1945337: WT1.LPS.T3; Mus musculus; RNA-Seq
library_construction_protocol: RLT lysis, 50% SPRI purification, Turbo DNase, MaximaRT, SAv purification 25% (final 12.5% RNA lysate), Random hexamer 2nd strand cDNA, 2ng ds_cDNA tagmented for 12 minutes and run for 15 cycles PCR according to Nextera manufactures protocol Modified SCRB-Seq, Soumillon, M., Cacchiarelli, D., Semrau, S., Oudenaarden, A. van & Mikkelsen, T. S. Characterization of directed differentiation by high-throughput single-cell RNA-Seq. bioRxiv 003236 (2014). doi:10.1101/003236; Modified Nextera protocol to PCR enrichonly  the 3' end of mRNA transcripts and create digital gene expression libraries for Illumina sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1945337