title: ExomeSeq of human HNSCC cell line: VUMCSCC4149: Sample VUMC-SCC-4149
design: We used Applied Biosystem by Life Technologies Fragment Library Preparation 5500 Series SOLiD™ Systems Publication, Part Number 4460960 Rev. A and TargetSeq™ Exome Enrichment System, Catalog Numbers A14060, Publication Part Number MAN0004396. 3ug of genomic DNA were fragmented, end-repaired, size-selected, dA-tailed, ligated to P1-T adaptor and Barcode-T, and PCR amplified (~6~ cycles). The DNA libraries were purified and captured using TargetSeq™ Exome Probe Pool provided in the TargetSeq™ Exome Enrichment System kit. The captured products were multiplexed, further amplified and sequenced on the SOLiD 5500 system with protocol 4456991 Rev. J.
attributes.tag: alignment_software
attributes.value: LifeScope v.2.5 Genomic Analysis Software