title: Middle ear effusion WGS 16: Sample 011_Sample16_C-29763764
design: "Shotgun sequencing using a Truseq Nano prep kit (Illumina, San Diego, CA). Genomic libraries were validated (concentration and size) with the Qubit and 2100 Bioanalyzer.  Sequenced on the Nextseq 500 using the 2x151 basepair paired end protocol, after an initial loading titration trial sequencing run. Raw reads (FASTQ format) were preprocessed (QC) using PRINSEQ-lite 0.20.4 (trimming reads and bases < 25 PHRED, minimum length=100, removing exact duplicates, reads with undetermined bases, and low complexity reads using Dust filter=25). Filtered (paired and unpaired reads) were aligned to the human genome (hg19) using Bowtie2 (--very-sensitive). Each sample has 4 technical replicates."