title: Honey 454 H8 rbcL
design: DNA amplified using the rbcL DNA barcode region (de Vere et al. 2012, PLoS ONE 7:e37945). Two step PCR, first PCR rbcL primers modified with M13 tails. PCR2 using unique 5bp adapter tags with M13 tails. Samples pooled and pyrosequenced on a Roche/454 GS FLX Titanium 1/4 plate.