title: GSM3854931: Light4-2 total RNA; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Roots were immeadiately frozen in liquid N2 (two independent biological samples, composed of approximately 70 seedling each) and total RNA was isolated using NucleoSpin® RNA Macherey-Nagel) according to the manufacturer's instructions. RNA concentrations were verified using the Nanodrop spectrophotometer (Thermo Scientific). Samples with concentrations ranging from 250 ng to 1 µg of RNA were precipitated with 3M NaOAc, 5mg/mL glycogen and ethanol and the pellet was kept in 70% ethanol for shipping. RNAseq sequencing was performed by Fasteris SA, Switzerland. Library preparation followed RiboZero treatment.
attributes.tag: GEO Accession
attributes.value: GSM3854931