title: WGS of Staphylococcus aureus / human adult male wound
design: Bacterial DNA was extracted by using Qiagen Kit instructors. One ng of genomic DNA was used as input for the Nextera XT kit(Illumina), followed by sample barcoding and amplification with12cyclesof PCR. Libraries were quantified on the Bioanalyzer (Agilent Technologies) and combined in an equimolar mixture. Next-generation sequencing (NGS) libraries were sequenced on a single run on the Illumina MiSeq instrument (300bp paired-end reads