title: Illumina HiSeq 2500 sequencing; ChIP-seq analyses of CTCF binding in liver samples from CAST/EiJ and SPRET/EiJ (Mus spretus) mice, and of the cohesin subunit RAD21 in C57BL/6J mouse liver
design: ChIP-seq analyses of CTCF binding in liver samples from CAST/EiJ and SPRET/EiJ (Mus spretus) mice, and of the cohesin subunit RAD21 in C57BL/6J mouse liver
library_construction_protocol: Liver sample was collected from one Mus musculus C57BL/6J individual Sample preparation was performed as described in Schmidt et al., 2012 (PMID: 22244452). Chromatin immunoprecipitation was performed as described in Schmidt et al., 2012 ((PMID: 22244452)) using 10Î¼g RAD21 antibody (Abcam, ab992, lot GR12688-8).   Immunoprecipitated DNA was used for library preparation using the ThruPLEX DNA-Seq library preparation protocol (Rubicon Genomics, UK). Library fragment size was determined using a 2100 Bioanalyzer (Agilent).
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: RAD21
attributes.tag: Experimental Factor: organism
attributes.value: Mus musculus domesticus