title: H3K27me3 ChIP-seq in in BAP1 KO mESCs upon 72h of ATRA  differentiation rep1
design: ChIP assays were performed according to the standard  protocols. Chromatin were crosslinked in 1% formaldehyde (Sigma Aldrich)  directly in the dish, and DNA was sonicated to 200 to 400 bp fragments by  sonication (Bioruptor, Diagenode). 50 to 1000 ug of chromatin of mESCs (with  spike-in Drosophila chromatin) were incubated with the various antibodies.  ChIP-seq libraries were prepared according to the standard protocols (NEB and  Illumina)