title: Illumina HiSeq 4000 sequencing; Exploiting evolutionary steering to induce collateral drug sensitivity in cancer
design: Exploiting evolutionary steering to induce collateral drug sensitivity in cancer
library_construction_protocol: Single cells were prepared from POT, GEF1 and TRM4 cells. After centrifugation, single cells were washed with PBS and were re-suspended with a buffer (Ca++/Mg++ free PBS + 0.04% BSA) at 1,000 cells/µl. Viability was confirmed to be > 90% in all samples using acridine orange/propidium iodide dye with LUNA-FL Dual Fluorescence Cell Counter (Logos Biosystems, L20001). Single cell suspensions were loaded on a Chromium Single Cell 3' Chip (10X Genomics) and were run in the Chromium Controller to generate single-cell gel bead-in-emulsions using the 10X genomics 3' Chromium v2.0 platform as per manufacturer's instructions. Single-cell RNA-seq libraries were prepared according to the manufacturer's protocol and the library quality was confirmed with a Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627) and a Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851).
attributes.tag: Experimental Factor: compound
attributes.value: none
attributes.tag: Experimental Factor: time
attributes.value: 0