title: GSM1289406: RNAseR.Hela.Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: A standard TRIzol extraction was conducted and RNA resuspended in 200ul of RNase free water. Purity was confirmed by NanoDrop (Thermo scientific). RNA was DNase treated with Turbo™ DNase (Life Technologies), repurified with phenol/chloroform, nanodroppped again and the integrity of the RNA confirmed by Agilent 2100 Bioanalyzer (Agilent Technologies). RNA sequencing libraries were made with the TruSeq® RNA Sample Preparation v2 Kit (Illumina). Given the RNA was previously ribodepleted, library preparation was begun at the fragmentation step and the standard procedure followed. Successful generation of sequencing libraries from digested and RNaseR negative mock-digestion samples were confirmed by Agilent 2100 Bioanalyzer. Samples were sequenced on an Illumnia® HiSeq. Given the very small amount of RNA remaining after RNAseR digestion, multiple digested samples were combined to reach the minimum yield requirement for sequencing library preparation. Where necessary samples digested with different conditions that all provided satisfactory digestion were also pooled.
attributes.tag: GEO Accession
attributes.value: GSM1289406