title: GSM1283055: H - 380 ppm; Clio pyramidata; RNA-Seq
library_construction_protocol: In the lab frozen individuals were weighed then removed from their shell to avoid both the possible pH interference due to the calcium carbonate of the structure and to avoid contamination by other taxa trapped in the shell. RNA was extracted using an RNA Purezol or the E.Z.N.A. Mollusc RNA Kit (Omega Biotek), and then eluted in 25-40 µL molecular biology grade water water. Libraries were constructed using Tru-Seq RNA Sample Prep Kits by the commericail providers at the Genomic Sequences Laboratory of Hudson Alpha. These were then multiplexed and sequenced in replicate on two lanes of the Illumina platform (v. 1.9) as a 50bp paired-end sequencing HiSeq run.
attributes.tag: GEO Accession
attributes.value: GSM1283055