title: Illumina MiSeq sequencing; qiita_ptid_5458:12033.LAC39.w6.E25
design: Infant monkeys (from birth to age of 16 weeks) consumed either exclusive breastmilk or infant formula (standard or reduce protein, with or without additional of free amino acids)
library_construction_protocol: Sequences were amplified using F515/R806 primer pair to target V4 region of 16S rRNA gene Both forward and reverse primers were modified to contain an illumina adapter region for sequencing on the illumina GaIIx platform and on the forward primer site, an 8 bp Hamming error-correcting barcode was used to enable sample multiplexing.
attributes.tag: barcode
attributes.value: ACGTGTTG
attributes.tag: center_name
attributes.value: UC Davis Slupsky Lab
attributes.tag: center_project_name
attributes.value: mead_johnson_Infant_monkey_project
attributes.tag: linker
attributes.value: GT
attributes.tag: linkerprimersequence
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: pcr_cond
attributes.value: 94degC_3min; denaturation:94degC_45sec; annealing:50degC_1min; elongation: 72degC_90sec; final elongation:72degC_10min; 40total cycles
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGCCAGCMGCCGCGGTAA
attributes.tag: reverseprimer
attributes.value: GGACTACHVGGTWTCTAAT
attributes.tag: run_center
attributes.value: UC Davis Genome Center
attributes.tag: run_date
attributes.value: 9/1/13
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4