title: 16S-rRNA gene sequences of buffalo Rumen Bacteria
design: Illumina MiSeq sequencing was carried out after library preparation from purified DNA using primers for V3-V4 region of 16S rRNA-gene. DNA libraries were sequenced using a 2x300 . DNA samples from three levels of Moringa oil (0, 1 and 4%) were selected for sequencing of 16S rRNA gene to determine bacterial diversity .