title: GSM2062454: Heat_3; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted with DNeasy Blood and Tissue Kit (Qiagen) following manufacturer’s instructions. Full details can be found in Olova et al. (2016). BS-seq: DNA samples were fragmented by sonication (Covaris) and adaptor ligated (using Illumina supplied methylated adaptors and NEB Next library preparation kit). Subsequently, DNA was bisulfite-treated using the Imprint kit (Sigma), EpiTect kit (Qiagen), EZ DNA Methylation Kit (Zymo). Final library amplification (15 cycles) was done using Pfu Turbo Cx (Agilent) and iPCRtag primers, after which the libraries were purified with AMPure XP beads (Agencourt). MeDIP-seq: DNA samples were fragmented by sonication (Covaris) and adaptor ligated (using Illumina TruSeq indexed adaptors and NEB Next library preparation kit). Subsequently, DNA samples were pooled, denatured and the input sample was sequenced directly without immuno-precipitation, after 6 cycles of amplification with NEB Next 2x Phusion mix and purification with AMPure XP beads (Agencourt).
attributes.tag: GEO Accession
attributes.value: GSM2062454