title: GSM4223368: HHSEC Fetal 11605; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Qiagen kit was used for extraction Bisulfite conversion of purified gDNA with EpiTect Fast chemistry and subsequent library generation: i) Whole genome bisulfite library generation using QIAseq Methyl Library Kit, which include random primed double strand synthesis, adapter ligation and library aplification, ii) targeted methylation sequencing using QIAseq Methyl Panel Kit as well as TrueSeq DNA Methylation Kit, which include adapter ligation introducing the frist sequencing index and UMI sequence, target enrichment using specific primers (custom Panel CMHS-10010Z-102) and final library amplification introducing second library index.
attributes.tag: GEO Accession
attributes.value: GSM4223368