title: GSM2831249: isk_4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted according to manufacturer's instructions (PicoPure RNA isolation kit, ThermoFisher Scientific, USA) and all samples were DNase-treated (ThermoFisher Scientific, USA). Due to low amount of input material, pre-amplification using the Ovation¨ RNASeq System V2 was performed. Total RNA was used for first strand cDNA synthesis, using both poly(T) and random primers, followed by second strand synthesis and isothermal strand-displacement amplification. For library preparation, the Illumina Nextera¨ XT DNA sample preparation protocol (Part # 15031942 Rev. C) was used, with 1 ng cDNA input. After validation (Agilent 2200 TapeStation) and quantification (Invitrogen Qubit System) all 5 transcriptome libraries were pooled.
attributes.tag: GEO Accession
attributes.value: GSM2831249