title: GSM3544644: PC3-control-rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from prostate cancer cells (including PC3-NC, PC3-OE-TRIB1; DU145-NC, DU145-OE-TRIB1) were isolated by TRIzol (No.:15596026, Thermo Fisher Scientific, USA) and extracted by chloroform. RNA degradation and contamination were monitored on 1% agarose gels and purity was checked by the NanoPhotometer spectrophotometer (IMPLEN, CA USA). RNA concentration was measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer(Life Technologies, CA, USA) and integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzeer 2100 system (Agilent Technologies, CA, USA ). Sequencing libraries were generated using NEBNest UltraTM RNA Library Prep Kit for IlluminaR (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
attributes.tag: GEO Accession
attributes.value: GSM3544644