title: GSM2831346: NPC_XY8 rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq data for NPC  cell lines was generated using the TruSeq protocol (Illumina) following ribosomal RNA depletion. Briefly, 400 ng of RNA was fragmented and then reverse transcribed using SuperScript III.  After second strand synthesis, RNA cDNA was A-tailed and adapters were ligated.  DNA was cleaned up using Ampure XP beads and then PCR amplified using the TruSeq PCR cocktail.  Libraries were sequenced on a HiSeq instrument with 2x75 bp reads.
attributes.tag: GEO Accession
attributes.value: GSM2831346