title: GSM2065966: 4C Dup-L LacZ; Mus musculus; OTHER
library_construction_protocol: 4C-seq libraries were generated from microdissected tissues or cells as described previously (van de Werken et al., 2012). DpnII was used as primary, BfaI as secondary restriction enzymes (Csp6I and NlaIII for human samples). For each viewpoint, a total of 1.6 mg of each library was amplified in 8 or 16 PCR reactions, depending on PCR efficiencies. Samples were multiplexed and sequenced with Ilumina Hi-Seq technology. The following primers were used: Sox9-TSS_1 CCACGAAAGTGTGTTTATATTCT   Sox9-TSS_2 TGGCTATTGTTTGTAAATCTCTT  Kcnj2-TSS_1 CGGTGGTGGAGAGAAAGA   Kcnj2-TSS_2 TCACCAAACAACCTCTACAAA  LacZ_1 TCCGACTTCAACTGTAGGG  LacZ_2 GCAGAGCCATCTATTGCTTA  BOR-Sox9tel_1 TTTGACTCCAAGGACCAGA  BOR-Sox9tel_2 TGTGTAAAGGCGGACAGA  Sox9-TAD_1 AACTGGGTATAGTGTGGAGAGA  Sox9-TAD_2 GGACATCAATGGAACATAGC  BOR-Kcnj/Sox9_1 AATGCTGTGGACATCCTG  BOR-Kcnj/Sox9_2 TCCGTCAAGTTCCATGTG  Kcnj-TAD_1 TCTGTGCCAAATGAACTATTCT  Kcnj-TAD_2 AGCACCATCTGTCCTCCTAT  BOR-Kcnjcen_1 AAGAAGAGGGGCTCAAAAT  BOR-Kcnjcen_2 CTGATTGGCATGGGATAGT  SOX9-TSS_1 TCCAAGTGTGTAAGTTTGTCG  SOX9-TSS_2 AATCTCCTGGACCCCTTC  KCNJ2-TSS_1 TTCAGTGAACGTATTTGTTGAA  KCNJ2-TSS_2 GAGCGTTTGATGTTTTACCA Samples were multiplexed and sequenced with Ilumina Hi-Seq technology using 100bp paired-end or single end reads according to the manufacturer´s protocol.
attributes.tag: GEO Accession
attributes.value: GSM2065966