title: GSM1290623: SUPeR-seq_2-cell2-2; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were picked into the lysis buffer which contained Rnase inhibitor to prevent RNA degration Cells were picked into the lysis buffer to release all RNAs; then the released RNAs were reverse transcribed with SuperScript III(Invitrogen), then digested the unreacted primers with ExoSAP-IT(USB) and tailed the first strand cDNA with a poly(A) sequence with terminal deoxynucleotidyl transferase (TdT, Invitrogen) , then used poly(T) primers to synthesize the sencond strand cDNA.the cDNAs were then enriched by two rounds of PCR amplifications each with size-selection by 2% agarose gel recovery then the library construction waa conducted following the illumina library preparing protocol. The quality-ensured libraries were used for pair-end deep sequencing on Illumina HiSeq2000 and Illumina HiSeq2500 Sequencers.
attributes.tag: GEO Accession
attributes.value: GSM1290623