title: BGISEQ-500 paired end sequencing; RNA-Seq of enteroids from wild-type and Tnfaip8 knockout mice.
design: RNA-Seq of enteroids from wild-type and Tnfaip8 knockout mice.
library_construction_protocol: RNAs were collected from MDSC using RNAeasy kit. rRNA depletion or Oligo dT Selection to enrich the mRNA RNA fragment and reverse transcription, End repair, add A and adaptor ligation, RCR amplification, Single strand separation and cyclization, DNA nanoball synthesis.
attributes.tag: Experimental Factor: genotype
attributes.value: Tnfaip8 -/-