title: GSM3611769: SAM24348567: Vemu Regressed; Mus musculus; RNA-Seq
library_construction_protocol: Samples were processed for single cell RNA sequencing (scRNAseq) using the Chromium Single Cell 3' Library and Gel bead kit v2, following the manufacturer's manual (10x Genomics, San Francisco, CA). Cell density and viability of the single-cell suspensions were determined by Vi-CELL XR cell counter (Beckman Coulter). All of the processed samples had very high percentage of viable cells. Cell density was used to impute the volume of single cell suspension needed in the reverse transcription (RT) master mix, aiming to achieve ~6,000 cells per sample. cDNAs and libraries were prepared following the manufacturer's manual (10x Genomics, San Francisco, CA). Libraries were profiled by Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, Santa Clara, CA) and quantified using Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA). Each library was sequenced in one lane of HiSeq4000 (Illumina, San Diego, CA) following the manufacturer's sequencing specification (10x Genomics, San Francisco, CA). cDNAs and libraries were prepared according to manufacturer instructions (10× Genomics)
attributes.tag: GEO Accession
attributes.value: GSM3611769