title: GSM3388397: RNAseq_D7P14_pMIG_ProgenitorLike_3; Mus musculus; RNA-Seq
library_construction_protocol: cell isolation protocol: Cells from spleen were obtained by mechanical disruption. Isolated cells were further sorted based on their surface markers. Lymphocytic choriomeningitis virus infection protocol: For acute viral infections, mice were intravenously (i.v.) injected with 2×10^5 plaque-forming unit (PFU) LCMV Armstrong. For chronic viral infections, mice were intravenously (i.v.) injected with 2×10^6 PFU LCMV clone 13. 50,000 P14 cells were sorted and resuspended in Qiazol (Qiagen). Total RNA was extracted with miRNeasy Mini kit (Qiagen). mRNA was selected using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). RNAseq libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for (NEB) according to manufacturer's instruction. The purified libraries were multiplexed and captured on an Illumina flow cell for cluster generation. Libraries were sequenced for 50 single read cycles on HiSeq 3000  according to the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM3388397