title: The assessment of the effect of alkali ballast water treatment on microbial community diversity Sample 46P_Pyro
design: Samples of Intake and NaOH treated ballast water were collected in a Great Lakes ship. The subset of the ballast water samples were PCR-amplified with barcoded universal primers targeting the V1-V2 region of the 16S rRNA gene using 27F and 338R primers. The PCR products were purified and then pooled in approximately equimolar amounts. The pooled sample was sequenced by pyrosequencing on a Roche 454 Genome Sequencer FLX. Raw reads were processed using RDP pipeline version 10 to sort the sequence data by barcodes, to trim barcode and forward primer sequences, and to filter out low quality sequences (qual below 20) and short reads (length below 150bp).