title: GSM1288667: miRNA-seq_ESC; Macaca mulatta; RNA-Seq
library_construction_protocol: According to the manufacturer’s instructions (illumina), mRNA was purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented into small pieces. The cleaved RNA fragments were converted to the first strand cDNA followed by generation of double strand cDNAs. These cDNA fragments went through end repair course by adding a single ‘A’ base as well as the adapter ligation. For miRNA sequencing, total RNA was ligated to a pair of adaptors at the 5’ and 3’ends according to the manufacturer’s instructions (Illumina). RNA molecules were converted to cDNA and amplified by RT-PCR using adaptor primers. The libraries prepared from samples of ESCs, R-NSCP1, R-NSCP6, and NPCs were used for paired-end mRNA sequencing by Illumina HiSeq 2000 and single-end miRNA sequencing by  Illumina HiSeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM1288667