title: AMPLICON of pharyngeal metagenome: infant human: Sample 1450.A
design: ','DNA was extracted from 150 µl sample, using the PowerMag® Soil DNA         Isolation Kit optimized for epMotion® and the epMotion® robotic platform model. Two-step PCR         of the v4 region was performed, First 30 cycles with broad range primers (515F and 806R),         then 15 cycles to add sequencing primers and adaptors. 250 bp Paired-end sequencing, of up         to 192 samples, were performed on the Illumina MiSeq System using MiSeq Reagent Kits         v2