title: GSM3930594: MB ni (replicate 2); Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using GenElute Total RNA Miniprep kit (Sigma-Aldrich) according to instructions Library preparation for RNA sequencing was performed using the TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, USA; Cat. N°RS-122-2002), starting from 1,000 ng of total RNA. Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega, Mannheim, Germany). The size range of the final cDNA libraries (average 350 bp) was determined in DNA 1000 chips using the Bioanalyzer 2100. CDNA libraries were amplified and sequenced by using the cBot and HiSeq2000 from Illumina.
attributes.tag: GEO Accession
attributes.value: GSM3930594