title: microbial community analysis of petroleum reservoir
design: V3 regions of 16S rRNA genes were amplified by primer P2 (5’-ATTACCGCGGCTGCT-3’) and P3 (5’-CCTACGGGAGGCAGCAG-3’) for bacteria and 344(5’-ACGGGGHGCAGCAGGCGCGA-3’) 522(5’-GWATTACCGCGGCKGCTG-3’) for archaea, which was tagged with an 8-base (2 protection bases and 6 barcode) to provide primer-specific individual PCRs for different samples. The sequences with no more than one base missing,extra or mismatch between the third and the eighth base of the 8-base barcode, were considered as useful sequences. Extra procedure were needed in actual analysis. The everage length of the useful sequences were 100bp. The barcode of each sample used was shown below.  Sample Name    Barcode  6-413 AAGTAGAG  5-14 AATCTGTG  7011 AATAGACG  8X815 AACATCAG  9-14 AACTCTAT  5G13 AATCACTG  3C15 AACTACAT  3-1 AAGACTCT  z3-X24 AACGAGCG  z3-14 AAGTATGT  z3h2o AAGCTATG  c6-15  AATGCTCT  c6-X17  AACTGAGT  CDh2o AACATATG  CD19-4 AACAGATG  MD 1 AAAGATAT  MD 5 AACAGACT  MD 6 AACATCTG  MD 12 AACACACT  SD 1 AATCGCAT  SD 6 AATAGCTG  SD 12 AAGCACAT  SD18 AACAGAGT  MD18 AACATACT  6-413 ACTCGTCT  5-14 ACGTGCAT  7011 ACAGCTAG  8X815 AGTGTGAG  9-14 AGTGCTAG  5G13 AGTGCACT  3C15 AGTATCAT  zhan3-1 AGCGTGTG  zhan3-X24 AGCGCTCT  zhan3-14 AGCGATGT  zhan3-H2O AGATCTCT  6-15 1-2 AGATCATG  6-X17 1-2 AGAGCTCG  CDD H2O ATACGTAT  CDD 19-4 ATATGCGT  SD18 ACACGTCT  MD18 ACACTACT  MD 1 ATATCGCT  MD 5 ATGACTGT  MD 6 ATGATACG  MD 12 ATGATACT  MD18 AAACACTG  SD 1 ATGCTCAT  SD 6 ATAGTGAT  SD 12 ATGTATCT  SD18 AATGTCGT