title: DNA methylation level of queen bee: brain, ovary and thorax of each newly emerged queen
design: The cDNA library was constructed following the manufacturers instructions for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530S), NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), NEBNext Multiplex Oligos for IlluminaIndex Primers Set1(NEB, E7335S) and the NEBNext Multi_x005f_x005f_x0002_plex Oligos for Illumina (NEB, E7500S).