title: "ONT sequecing of yeast W303, sample STUART_103114_W303_333pore_R7.3"
design: "DNA was sheared following Covaris instructions 10kb, 2ug DNA input then split into two equal aliquots prior to ligation, adapter mix and hairpin adapter added at same time, wash buffer beads spiked with BSA. No his beads used 100ng DNA estimated added to flow cell, otherwise standard ONT R7.3 protocol "