title: GSM2752506: SU_Tag_seq; Rattus norvegicus; RNA-Seq
library_construction_protocol: Urogenital tracts were removed from day of birth Wistar rats and microdissected into VMP and SU components using a Leica MZ6 dissection microscope. For RNA-sequencing only, VMP and SU tissues were disscociated with 2mg/mL Collagenase 1A (Sigma-Aldrich, Missouri, USA) for 60 minutes at 37 degrees C. Dispersed fibroblasts were separated by centrifugation and passing through a 0.7um cell strainer (Falcon, Corning, New York, USA) Microdissected tissues (VMP and SU) were processed for digital gene expression Tag-profiling using NlaIII and a protocol provided by Illumina followed by sequencing on an llumina GAIIX (1x 50 SE) at one lane per sample (25-30m reads). PolyA+ RNA was purified, cDNA synthesised, digested with NlaIII and ligated to Adaptor 1 (containing an Mme1 site). Samples were digested with Mme1, ligated to Adaptor 2, and PCR amplified, followed by gel electrophoresis and purification of 85bp fragments that were sequenced
attributes.tag: GEO Accession
attributes.value: GSM2752506