title: GSM1290714: Ponkan_degradome; Citrus reticulata; RNA-Seq
library_construction_protocol: Total RNA was isolated from all of the samples using modified trizol method according to Liu et al.,Journal of Huazhong Agricultural University,2006 Small RNA sequencing was carried out using Illumina sequencing technology by the Beijing Genomics institute (BGI). Firstly, RNAs of 18-30 nt long were isolated by size fraction with 15% Tris-borate-EDTA urea polyacrylamide gel. Subsequently, they were ligated to 5' and 3' RNA adaptors (Illumina) by T4 RNA ligase. After that, the adapter-ligated RNA molecules were reverse-transcribed into cDNA by Super Script III using a primer specific to the 3’ adapter, followed by PCR amplification with two primers that annealed to the ends of the adapters. These PCR products constituted the sequencing library.
attributes.tag: GEO Accession
attributes.value: GSM1290714