title: GSM4674951: NW9; Homo sapiens; RNA-Seq
library_construction_protocol: CD19+ CD27+ memory B cells (MBC) were sorted into 4 subsets defined by CD45RB and CD69 from spleen of four individual donors (D193 (D1), D256 (D2), D260 (D3), and D305 (D4), see Table S1). RNA was isolated from cell pellets using the RNeasy Mini or Micro Kit (Qiagen), and RNA concentration and quality was assessed using an Agileumnt 2100 Bioanalyzer instrument (Agileumnt Technologies). Libraries were prepared using the Illumina TruSeq Total RNA kit with ribosomal depletion or the SMART-Seq v4 Ultra Low Input RNA Kit for sequencing and Illumina Nextera XT DNA Library preparation kit following the manufacturer's protocol
attributes.tag: GEO Accession
attributes.value: GSM4674951