title: GSM2671595: Vc_self_4; Vuilleminia comedens; RNA-Seq
library_construction_protocol: The half of each wood block which was in contact with the other block was ground into sawdust using a block plane. Total RNA was extracted using the RNA Powersoil kit from MoBio (California, USA), according to the manufacturer’s instructions. Samples were quality controlled and quantified using the Nanodrop (ThermoFisher Scientific, Massachusetts, USA) and the Agilent Bioanalyzer (California, USA). Dynabeads mRNA purification kit (Ambion, Massachusetts, USA) was used to isolate mRNA from the extracted total RNA. The cDNA library was generated and amplified using the SMARTer stranded RNA-seq kit from Clontech (California, USA) according to the manufacturer’s instructions, followed by purification using the Agencourt AMPure PCR purification kit (California, USA). The library preparation was validated using the High Sensitivity DNA kit for the Agilent Bioanalyzer. The 3 independent libraries were kept at -80oC before sequencing by the Centre for Genomic Research (CGR) at Liverpool University. Paired-end sequencing (2x125 bp) of indexed libraries was conducted using the Illumina HiSeq 2500 platform, sequenced with SBS V4 chemistry.
attributes.tag: GEO Accession
attributes.value: GSM2671595