title: GSM1707598: Primed_RNAseq_technicalReplicate2; Homo sapiens; RNA-Seq
library_construction_protocol: 6 million naive or primed hESCs were used for each RNA extraction. Total RNA was purified using the mirVana™ miRNA Isolation Kit (Life Technologies, AM1560) following the manufacturer’s instructions. 1 μg of total RNA was used for the RNA-seq library construction. A technical replicate was performed for both naïve and primed hESCs. Polyadenylated RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101). The RNA-seq libraries were sequenced on the Illumina HiSeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM1707598