title: Hessian fly gut microbiome
design: Gut DNA from three different larval instars of Hessian fly (Mayetiola destructor) was amplified using V3-region (16S rRNA gene) specific primers (U341F-U529R) separately. The reverse primer (529R) was barcoded with a 5-bases identifier. The sequencing was performed on GS-FLX sequencer (454 platform). the fastq file was processed using customized perl scripts to demultiplex the data and to remove low quality reads. Further, reads were discarded if they were <90 bases or >135 bases, or with one or more undetermined or ambiguous nucleotides, or with no valid barcode. Note: The data were submitted to the SRA without quality scores. Quality scores were arbitrarily set at Phred 25 for processing.