title: GSM2751736: G004_24h_C3; Coryphaena hippurus; miRNA-Seq
library_construction_protocol: Thirty pooled embryos or larvae per replicate were homogenized with a Kontes Pellet Pestle Cordless Motor (Sigma-Aldrich, St. Louis, Missouri) in Qiazol. Samples were isolated using the miRNeasy mini kit and RNeasy Mini Elute Cleanup Kit from Qiagen (Valenica, CA). miRNA libraries were made using the New England Biolabs NEBNext Multiplex Small RNA Sample Prep kit (Ipswich, MA) following the manufacturer’s protocol.  The PCR amplified cDNA libraries were then purified using the Qiagen QIAQuick PCR Purification Kit (Valencia, CA) according to the manufacturer’s manual. The purified libraries were size-selected using Beckman Coulter SPRIselect beads (Indianapolis, IN).  The size distribution and concentration of the libraries were determined using the Agilent High Sensitivity DNA Assay Chip (Santa Clara, CA). Thirty pooled embryos or larvae per replicate were homogenized with a Kontes Pellet Pestle Cordless Motor (Sigma-Aldrich, St. Louis, Missouri) in Qiazol. Samples were isolated using the miRNeasy mini kit and RNeasy Mini Elute Cleanup Kit from Qiagen (Valenica, CA). miRNA libraries were made using the New England Biolabs NEBNext Multiplex Small RNA Sample Prep kit (Ipswich, MA) following the manufacturer’s protocol.  The PCR amplified cDNA libraries were then purified using the Qiagen QIAQuick PCR Purification Kit (Valencia, CA) according to the manufacturer’s manual. The purified libraries were size-selected using Beckman Coulter SPRIselect beads (Indianapolis, IN).  The size distribution and concentration of the libraries were determined using the Agilent High Sensitivity DNA Assay Chip (Santa Clara, CA).
attributes.tag: GEO Accession
attributes.value: GSM2751736