title: GSM4222876: wt E9.5 single-cell mRNA, S, replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol according to the manufacturer's instructions (Invitrogen) The published Drop-seq protocol (Macosko et al., 2015), with a few revisions to the published protocol: we added 0.5M NaCl to the lysis buffer to enhance RNA binding, 2) used beads manufactured by Biosearch (non-catalog), 3) used a flow-rate of 3ml/hr for both the cell suspension and beads as well as a flow-rate of 11ml/hr for the oil, 4) a custom fabricated PDMS device that generates droplets 120um in diameter and 5) used 6000 beads per PCR reaction. 100,000 cells were inputted from each phase of each of the two E9.5 samples. Viability of all input populations exceeded 90%. Cells were resuspended at 100,000 cells per ml, with beads at 120,000 beads per ml to target 5% cell capture.
attributes.tag: GEO Accession
attributes.value: GSM4222876