title: GSM2482839: PPC_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed and RNA isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. The amount of total RNA was quantified using Qubit 2.0 Fluorometric Quantitation system and the RNA integrity number (RIN) was determined using Experion Automated Electrophoresis System RNA-Seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit using Sciclone and Zephyr liquid handling robotics. Library amount was quantified using the Qubit 2.0 Fluorometric Quantitation system and the size distribution was assessed using Experion Automated Electrophoresis System
attributes.tag: GEO Accession
attributes.value: GSM2482839