title: GSM3611454: 6-day ML210 treatment; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA from cell pellets was purified using the QIAamp DNA Blood Maxi/Midi/Mini kits (Qiagen) according to the manufacturer's protocols and quantified using a Nanodrop 2000 (Thermo Fisher Scientific). sgRNA cassettes were PCR-amplified and barcoded with sequencing adaptors utilizing ExTaq DNA Polymerase (Clontech). PCR products were purified with Agencourt AMPure XP SPRI beads (Beckman Coulter A63880) according to the manufacturer's instructions, quantified using a Nanodrop 2000, pooled into a master sequencing pool, and sequenced on a MiSeq sequencer (Illumina) with 300nt single-end reads, loaded at 60% with a 5% spike-in of PhiX DNA.
attributes.tag: GEO Accession
attributes.value: GSM3611454