title: The transcriptome analysis of Oncomelania hupensis by next-generation sequencing
design: In brief, poly(A) mRNA were purified from Total RNA by using oligo (dT) magnetic beads, and chopped into short fragments using divalent cations under elevated temperature. The cleaved RNA fragments were reverse transcribed into first strand cDNA using random primers and then synthesized into double-stranded cDNA. From these cDNA, a paired-end library was synthesized for each group using the mRNA-Seq Sample Preparation Kit (Illumina)  according to the manufacturer's instructions. Short fragments were purified with QIAquick PCR Purification Kit (Qiagen) and for continued end repair and ‘A’ base addition. These DNA fragments were then ligated into adapters and purified through gel seperation. Finally, the adaptor-ligated libraries were amplified by PCR for sequencing.  There are three samples have been sequenced, and barcodes have been used during sequencing. Index for sample 1-3 is GTGGCC, ATTCCT and CAACTA