title: "ONT sequecing of yeast W303, sample STUART_182pore_250ng_091114"
design: "DNA was sheared following Covaris instructions 10kb, 2ug DNA input then split into two equal aliquots prior to ligation, adapter mix allowed to incubate for 5 minutes with ligase prior to adding HP adapter, 30min motor incubation, 250ng DNA estimated added to flow cell, otherwise standard ONT R7.0 protocol"