title: "ONT sequecing of yeast W303, sample KEVIN_111014_W303_431pore_72ng_R7.3"
design: "DNA was sheared following Covaris instructions 10kb, 2ug DNA input then split into two equal aliquots prior to ligation, adapter mix and hairpin adapter added at same time, wash buffer beads spiked with BSA. No his beads used 125ng DNA estimated added to flow cell, otherwise stadard ONT R7.3 protocol "