title: GSM3317189: RNA-Seq of Leghorn Chicken Harderian gland Individual 16 [1037]; Gallus gallus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the Harderian gland of four individuals per treatment and genetic line for each of the three time points. The Harderian gland was homogenized in ice cold TRIzol (ThermoFisher Cat#15596026) and processed using a standard phenol:chloroform method and precipitated in 100% ethanol. The RNA pellet was then dissolved into water and treated with DNase I (ThermoFisher Cat#EN0521). Strand specific RNA library preparation was prepared exactly as stated in the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB Cat#E7420S). Library validation and quantification was done using the Agilent Bioanalyzer High Sensitivity Kit (Agilent Cat#5067-4626) and Qubit dsDNA HS Assay kit (ThermoFisher Cat#Q32854).
attributes.tag: GEO Accession
attributes.value: GSM3317189