title: GIST whole genome sequencing
design: All tumours and corresponding normal tissues were homogenized and subsequently mixed with lysis buffer   (700 microlitre per gram tissue)  containing 2.1% (v/v) Proteinase K  (10mg/ml)  and 0.7% SDS in TE (pH 8.0).  Samples were incubated o/n at 37°C. Phenol extraction followed by butanol treatment and ethanol precipitation was performed  as described by Sambrook and Russell.  A DNA library generated from 3 microgram of DNA  was sequenced using 7 lanes per sample on an Illumina HiSeq 2000 machine.