title: Genome re-seq of S. pneumoniae D39V spd_0476 dnaA-Q247H
design: Genomic DNA was isolated from bacterial cultures grown in CY medium until OD=0.4. Cell were harvested, lysed using 0.025% Deoxycholate for 20min at 30C. DNA was precipitated with isopropanol and disolved in TE buffer. Further library preparation was performed by GATC Biotech.