title: RNA-seq of Bombyx mori: male fat body
design: For RNA sequnencing, total RNA was extracted from fat body at different development stage respectively. 1-17mg fat body were dissected in PBS and directly transferred to Trizol (Invitrogen, USA), then grinded with liquid nitrogen. RNA was extracted again trichloromethane, after precipitate by isopropanol and cleared by 75% ethanol, RNA samples were stored in a refrigerator at 80C for sequencing libraries. Each cDNA sample was clustered using Oligo-dT magnetic beads and then sequenced on the HiSeq2000 (Illumina, USA) platform using Single-read techology. All of the Raw data presented in this study have been deposited in the NCBI Short Read Archive (http://www.ncbi.nlm.nih.gov/sra/) under SRA accession number