title: GSM4223448: human K562 input-1; Homo sapiens; ChIP-Seq
library_construction_protocol: Briefly, 30X106 K562 cells were fixed with 1% formaldehyde, sonicated and immunoprecipitated with antibodies against LDB1 or ETO2 . Chromatin was pulled down using Dynabeads Protein G, washed twice with RIPA-LS, RIPA-HS, LiCL washing buffer, followed by one wash with 10mM TrisHCl pH8.0. Precipitated DNA was tagmentated on magnetic beads with 0.2ul of tagmentation enzyme (Nextera XT DNA Library Preparation Kit, Illumina # FC-131-1024) for 10 minutes at 37 degree while shaking. The beads with tagementated DNA were washed twice with RIPA-LS and TE buffers. The DNA was eluted and de-crosslinked in ChIP elution buffer (20 mM Tris, pH 7.5, 5 mM EDTA, pH 8.0, 50 mM NaCl, 1% SDS, and 50 μg/mL proteinase K) for 1 hour at 55 degree and incubated at degree  overnight. ChIP Libraries were prepared using Nextera XT DNA Library Preparation Kit, Illumina # FC-131-1024. Eluted DNA was purified using SRI beads (1.8x) and PCR amplified with Nextera primers. Libraries were purified (1.8x) and size selected (0.65x/ 0.15x) with SRI beads.
attributes.tag: GEO Accession
attributes.value: GSM4223448