title: GSM1623210: SDH; Physcomitrella patens; Bisulfite-Seq
library_construction_protocol: DNA extracted from  hydrated (HD), mild dehydrated (MDH), severe dehydrated (SDH), partially rehydrated (PRE) and fully rehydrated (FRE) gametophores of moss P. patens. Fragment genome DNA to 100-300bp by Sonication, and then DNA-end repair , 3’-dA overhang and ligation of methylated sequencing adaptors are conducted. Bisulfite treatment by ZYMO EZ DNA Methylation-Gold kit. Desalting, size selection, PCR amplification and size selection again. Qualified library for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1623210