title: WGS of H CC
design: Genomic DNA (3 g, quantitated by fluorometer and agarose gel) from 43 adrenal tumors, 25 buffy coat and the H295R cell line was fragmented and exome captured. Exome target capture and enrichment were performed using Agilent SureSelect Human All Exon V4 target enrichment kit (Agilent Technologies, Santa Clara, CA) which targeted 51 megabases of sequence (20,965 genes, and 334,378 exons and miRNAs). Exome libraries were prepared by following manufacturers recommended protocols. Sequencing was performed using an Illumina HiSeq2000 (Illumina, San Diego, CA), the sequencing was run as 2x100 base pairs with TruSeq V3-HS reagents. The HiSeq Real Time Analysis (RTA 1.12.4.2) was used for processing image files, and the Illumina CASAVA 1.8.2 was used to demultiplex and convert binary base calls and qualities to fastq format. The sequence reads were aligned to human genome (GRCh37/USCS hg19) reference using the CASAVA 1.8.2 alignment module (ELANDv2e gapped alignment).