title: T.hassleriana flower 1
design: For the floral transcriptome sequencing total RNA was extracted from the T. hassleriana  floral tissue using guanidium thiocyanate-phenol-chloroform extraction protocol (Chomczynski & Sacchi, 1987). The  polyA+  mRNA was isolated using the Oligotex mRNA Minikit (Qiagen, Hilden, Germany)  according to the manufacturer’s instruction. 200 ng of purified polyA+  mRNA was used to synthesize the cDNA for sequencing with  Roche Rapid Library kit (Roche) following the manual. A massively parallel pyrosequencing run was performed on a GS FLX using Titanium chemicals (Roche).