title: Transcriptome analysis of Chironomus riparius exposed to various environmental contaminants using 454 GS FLX
design: Approximately, 1μg of the final PCR product DNA was used to generate DNA library for Genome Sequencer FLX Titanium (Roche, Mannheim, GE). The fragments ends were polished (blunted), and short two adapters were ligated onto both ends and DNA sequencing was performed following protocols for the Genome Sequencer GS FLX System (Roche Diagnostic).