title: ITS-Seq: SY agricultural soil
design: The total community DNA was extracted from soils by use of a MoBio Power Soil DNA extraction kit. The ITS region was amplified in triplicate using the barcoded 2045F and 2390R primer set. PCR products were quantified, purified and sequenced by Illumina Hiseq 2500 platform for assessment of fungal diversity and community structure.