title: GSM4674918: adult human heart (Z6532N); Homo sapiens; RNA-Seq
library_construction_protocol: RNAs were extracted using a QIAGEN RNeasy Micro Kit following manufacturer's instructions. Libraries were prepared according to the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). Briefly, cDNA was generated from 10ng of total RNA. The resulted cDNA was amplified and barcoded using Ion AmpliSeq™ technology to accurately maintain expression levels of all targeted genes. Barcoded cDNA Libraries were quantified using Ion Library TaqMan Quantitation Kit (Thermo Fisher Scientific). Libraries were then diluted to 45 pM and pooled  by combining an equal volume of each.
attributes.tag: GEO Accession
attributes.value: GSM4674918