title: Metagenome of marine sediment: extracellular DNA
design: DNA samples were fragmented ultrasonically, and an average insert size of 550 bp was obtained. The fragments were adapter-ligated using TruSeq DNA PCR-Free Library Preparation Kit (Illumina) or KAPA Hyper Prep Kit, and amplified using KAPA HiFi HS ReadyMix, with 12-18 cycles. The libraries were sequenced on Illumina Miseq (paired 301-bp reads).