title: Ion Torrent PGM of Anigozanthos manglesii: leaf
design: DNA was sheared to approximately 300-400 bp using a S2 sonicator (Covaris, Woburn, Massachusetts, USA) and a single barcoded library was prepared using a NEBNext Ultra DNA Library Prep Kit (New England Biolabs, Ipswitch, Massachusetts, USA). Inserts sized 330-360bp were selected by gel excision (E-Gel, Invitrogen/Thermo Fisher Scientific, Waltham, Massachusetts, USA) and the libraries produced, assessed, and quantified using a Bioanalyzer 2100 (Aglient Techoogies, Santa Clara, California, USA). The final library was diluted to 9pM using a OneTouch 2 Template 400 kit (Life Technologies) and enriched. A Personal Genome Machine (PGM) semiconductor sequencer (Life Technologies, Carlsbad, California, USA) using 850 flows on a 316 sequencing chip  produced approximately 350-400bp read lengths. Signal processing, base-calling and quality trimming was conducted using the default settings on TorresntSuite 4.0, and library specific FASTQ files generated.