title: GSM4222010: A-myb-KO-PS-H3K27Ac-2; Mus musculus; ChIP-Seq
library_construction_protocol: For RNA-seq analysis, total RNA from ES cells on each well of 24 well plate was extracted using an RNeasy Plus Mini Kit (QIAGEN), with gnomic DNA elimination. For ChIP-seq analysis, to extract chromatin, isolated pachytene spermatocytes were suspended in 20 µl of Nuclei EZ Lysis Buffer (SIGMA), and extensively digested chromatin with 2 IU/µl Micrococcal Nuclease (MNase, NEB) at 37℃ for 5 min. RNA-seq library preparation was carried out using TruSeq Stranded mRNA Library Prep kit (Illumina) following manufacturer's instructions. ChIP-seq library preparation was carried out by NEBNext Ultra II DNA Library Prep Kit (NEB) following manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4222010