title: Marker gene sequencing of soil: relic DNA study: Sample S19_Tot_rep4.ITS
design: DNA extraction with MoBio PowerSoil kit. PCR/library prep using Earth Microbiome Project protocols. 16S primers = 515f/806r. ITS primers = ITS1f/ITS2. Note: reps are subsamples of the same field collection processed separately for all DNA extraction through library prep. 