title: 7tissues
design: 7tissues
library_construction_protocol: The original samples were collected from beef cattle at the Lacombe research station in Alberta (Canada),ﾠfollowing the guidelines of the Canadian Council on Animal Care (1993)ﾠand the protocol approved by the Lacombe Research Centre Animal Care Committee. mRNA from seven different tissues was extracted (adipose, muscle, hypothalamus, duodenum, liver, lung and kidney) from frozen tissues using TRIzol (Invitrogen). Sequencing libraries were constructed from each pooled tissue according to a standard protocol (mRNA Sequencing Sample Preparation Guide, Illumina, USA)
attributes.tag: Experimental Factor: organism part
attributes.value: adipose