title: De novo assembly and characterization of transcriptome during early-stage fruit development in two genotypes of Annona squqmosa L.-Sitafal_12DAP_ENR26SA01
design: Approximately 1µg total RNA was used for preparing mRNA sequencing library of each sample. Poly (A+) RNA was isolated from the total RNA mixture by using using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) following the manufacturer’s protocol.  The purified Poly (A+) RNA was sheared and cDNA library was prepared by using  NEBNext® mRNA Library Prep Reagent Set for 454™ (New England Biolabs) following the instruction manual. The cDNA libraries were sequenced on a 454 Genome Sequencer FLX+ platform (Roche, USA).