title: Transcriptome of Echmepteryx hageni: Sample Echag - Echmepteryx hageni
design: "Construction of libraries and sequencing on the 454 Genome Sequencer FLX Titanium system were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from total RNA, converted to double-stranded cDNA, nebulized, gel-purified, and ligated to Titanium adapters following the GS Titanium Library Preparation kit (454 Life Sciences, CT, USA)."" Sequencing was performed on the 454 Genome Sequencer FLX Titanium system. Signal processing and base calling were performed using the bundled 454 Data Analysis Software."