title: Illumina HiSeq 2500 sequencing; Chicken RNA-seq time-series of the development of seven major organs
design: Chicken RNA-seq time-series of the development of seven major organs
library_construction_protocol: The organs were dissected from 9 developmental stages (starting at e10 until P155). The dissected 'brain' samples correspond to the cerebral hemispheres and the 'cerebellum' samples to the cerebellum. In chicken only the left ovary develops and this was the one that was dissected. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol.  The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina)
attributes.tag: Experimental Factor: organism part
attributes.value: kidney
attributes.tag: Experimental Factor: developmental stage
attributes.value: postnatal day 0