title: MicroRNA expression profiles of skeletal muscle satellite cells during differentiation in bovine
design: Total RNA was extracted from MDSCs using TRI-reagent (Invitrogen, Carlsbad, CA) following manufacturers instructions. Subsequently, low molecular weight RNAs were separated by 15% polyacrylamide gelele ctrophoresis (PAGE ), and RNA molecules in the range of 18-30nt were enriched and ligated with proprietary adapters to the 50 and 30 termini. A reverse transcription react ion followed by low cycle PCR was performed to obtain sufficient product for Solexa technology (Beijing Genomics Institute, China).