title: GSM3930463: R059-Pano1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq samples were pelleted and lysed in Trizol reagent then subsequently precipitated and processed through a Zymo RNA Clean and Concentrator-5 column After extraction from Trizol, RNA-seq samples were poly(A) selected using Dynabeads mRNA Purification Kit (Life Tech) before fragmentation using Thermo AM8740 and Second Strand Synthesis. Libraries were end-repaired using T4 DNA Polymerase, E. coli Klenow fragment, and T4 polynucleotide kinase. DNA was then A-tailed using exo(-) Klenow fragment and ligated to adaptors from NEBNext MultiPlex Oligos using New England BioLabs Quick Ligase. Libraries were sequenced at 1x75 on an Illumina NextSeq 500