title: GSM1289382: RNA-seq 256-cell; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was isolated using the standard Trizol (Invitrogen) protocol. Two rounds of PolyA+-RNA purification were performed for each sample, using the PolyA(Purist)TM-MAG kit (Ambion). The quality of the RNA and lack of contaminating ribosomal RNA were confirmed using the Agilent 2100 Bioanalyzer. Strand-specific libraries for 76 bp paired-end sequencing were prepared according to a modified UTP-method (Parkhomchuk et al. 2009), as detailed in (Levin et al. 2010). Libraries were prepared using the Illumina sequencing library preparation protocol.
attributes.tag: GEO Accession
attributes.value: GSM1289382