title: Illumina HiSeq 2500 sequencing; Mouse RNA-seq time-series of the development of seven major organs
design: Mouse RNA-seq time-series of the development of seven major organs
library_construction_protocol: The organs were dissected from 14 developmental stages (starting at e10.5 until P63). Until e12.5 the dissected 'brain' samples correspond to the whole brain, and from e13.5 onwards they correspond to the cerebral hemispheres (without the olfactory bulbs). Early 'hindbrain' samples correspond to the prepontine hindbrain-enriched brain region (until e15.5) and from then onwards only to the cerebellum. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
attributes.tag: Experimental Factor: organism part
attributes.value: testis
attributes.tag: Experimental Factor: developmental stage
attributes.value: embryonic day 10.5