title: Illumina MiSeq sequencing; qiita_ptid_4493:2248.C1096.ONMN.2.20.14
design: The purpose of the present study is to gain initial insight into the role of alterations to the gut microbiome in the development of age-related physiological dysfunction in a setting of lifelong Western diet consumption, and to determine whether healthy lifestyle or dietary interventions that improve physiological function may be effective in part due to preservation or improvement in the composition of the gut microbiome.
library_construction_protocol: Illumina EMP protocol 515f, 806bcr amplification of 16S rRNA V4
attributes.tag: barcode
attributes.value: GTCGTCCAAATG
attributes.tag: center_name
attributes.value: CU Boulder
attributes.tag: center_project_name
attributes.value: seals aging microbiome mice
attributes.tag: experiment_center
attributes.value: CCME
attributes.tag: illumina_technology
attributes.value: MiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: miseq_runid
attributes.value: 140701_M00517_0195_000000000-A7H2B
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: physical_specimen_remaining
attributes.value: false
attributes.tag: primer
attributes.value: GT
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 7/1/14
attributes.tag: runid
attributes.value: 140701_M00517_0195_000000000-A7H2B
attributes.tag: samp_size
attributes.value: .1,g
attributes.tag: sample_center
attributes.value: CCME
attributes.tag: sample_type
attributes.value: XXQIITAXX
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4