title: Polypodum hydriforme transcriptomic reads
design: For P. hydriforme, RNA was extracted from multiple individuals of the elongate stolon developmental stage using TriReagent (Life Technologies, Grand Island, NY) following standard protocols. Extraction was followed by DNase treatment using the TURBO DNase kit (Ambion). P. hydriforme samples were prepared for sequencing by Mariya Shcheglovitova using the TruSeq RNA Sample Preparation Kit v2 (Illumina Inc., San Diego, CA).  RNA was chemically fragmented, using reagents supplied in the TruSeq kit, resulting in libraries with a median insert size of 155 bp.  Both samples were given a single lane and sequenced at the University of Massachusetts Medical School Molecular Biology Core Facility on an Illumina HiSeq 2000 machine.