title: Illumina HiSeq 2000 sequencing; qiita_ptid_65:2080.S39D435
design: In April-May 2012, we participated in a research cruise as part of the US CLIVAR/CO2 Repeat Hydrography program, onboard the UNOLS ships R/V Atlantis. The cruise was a repeat of World Ocean Circulation Experiment (WOCE) line A20 (longitude 52W), previously conducted in 2003. 10-L water samples were collected at a total of 83 stations at varying depth intervals, using a 36-Niskin bottle rosette. We took 0.5-1.0 L samples from the rosette roughly once per day at 16 stations throughout the cruise. At each station, water was taken from at least three distinct zones in the water column, based on data from the CTD: the middle of the mixed layer, the local oxygen minimum, and the middle of the bathypelagic zone. At 7 stations, samples were also drawn from the bottom-most bottle. Biomass was immediately collected on 0.2 um filters under vacuum filtration. SIP microcosms were set up at 13 stations using 13C sodium acetate, 13C urea, 13C sodium bicarbonate, 13C algal lipid/pigment extract, and 15N algal protein extract. The samples submitted to the Earth Microbiome Project represent T=0 time points for the SIP microcosms (before amendment) and extra samples taken for RNA/DNA analysis. Results from SIP are currently being processed.
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: CAGTCGTTAAGA
attributes.tag: center_name
attributes.value: ANL
attributes.tag: center_project_name
attributes.value: Seyler_North_Atlantic_water_column
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: MiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: ANL
attributes.tag: run_date
attributes.value: 2013
attributes.tag: run_prefix
attributes.value: Undetermined_S0_L001_R1
attributes.tag: samp_collect_device
attributes.value: niskin bottle
attributes.tag: samp_mat_process
attributes.value: OBI:liquid filter function
attributes.tag: samp_size
attributes.value: 0.5L
attributes.tag: sample_center
attributes.value: ANL
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4