title: H3K9me3 ChIP-Seq analysis of cortex neural stem and progenitor cells from HuFNSC01 (A03271)
design: H3K9me3 ChIP-Seq analysis of cortex neural stem and progenitor cells from HuFNSC01 (A03271) using Illumina HiSeq 2000
library_construction_protocol: Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: EXPERIMENT_TYPE
attributes.value: Histone H3K9me3
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
attributes.value: Fisher 550 Sonic Dismembrator
attributes.tag: EXTRACTION_PROTOCOL_SONICATION_CYCLES
attributes.value: 10 min
attributes.tag: CHIP_PROTOCOL
attributes.value: Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
attributes.tag: CHIP_PROTOCOL_CHROMATIN_AMOUNT
attributes.value: 5 ug
attributes.tag: SIZE_FRACTION
attributes.value: 74-314 bp
attributes.tag: CHIP_PROTOCOL_BEAD_TYPE
attributes.value: Sepharose A/G Bead Mix
attributes.tag: CHIP_PROTOCOL_BEAD_AMOUNT
attributes.value: 20 ul
attributes.tag: CHIP_PROTOCOL_ANTIBODY_AMOUNT
attributes.value: 5 ug
attributes.tag: CHIP_ANTIBODY
attributes.value: H3K9me3
attributes.tag: CHIP_ANTIBODY_PROVIDER
attributes.value: Diagenode
attributes.tag: CHIP_ANTIBODY_CATALOG
attributes.value: pAb-056-050
attributes.tag: CHIP_ANTIBODY_LOT
attributes.value: A93-0042