title: Illumina HiSeq2000 sequencing of Bambusa edulis transcriptome
design: The B. edulis tissue culture system was established by following our previous protocol. Multiple shoots were incubated in MS medium supplemented with 0.1 mg/l thidiazuron  to induce flowering. The inflorescences were subcultured in medium containing 5 mg/l napththalene acetic acid to induce roots, shoots and flowers. The total RNA of these organs were isolated using Trizol reagent (Invitrogen, Carlsbed, CA, USA), following the manufacturer’s instructions. The pooled RNA was used for NGS sequencing.