title: GSM3553136: ChIPseq_FLAG_in_DMS3-ZF_fwa_Figure2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: ChIPs were performed as described previously with minor modifications (Johnson et al., 2014). 2g of inflorescences were used for Flag, HA, NRPE1 and AGO ChIPs. 2g of 12 day-old seedlings were used for Pol II Ser5 ChIP. All samples were ground and crosslinked with 1% formaldehyde. Chromatin was sheared using a Bioruptor Plus (Diagenode) and immunoprecipitated with Anti-Flag M2 (Sigma), Anti-HA 3F10 (Roche), Anti-Pol II phospho Ser5 Ab5131 (Abcam), Anti-NRPE1 and NRPE1 preimmune serum for NRPE1 control (Liu et al., 2018), Anti-AGO4, Anti-AGO6 and Anti-AGO9 (provided by Dr Olivier Voinnet, ETH, Switzerland) antibodies. Libraries were prepared using the Ovation Ultra Low System V2 1-16 kit (NuGEN) following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3553136