title: mRNA seq for the study of early process of retinal regeneration
design: Total RNA was purified from target tissues conventionally and evaluated by Agilent 2100 Bioanalyzer (Agilent Technology, Santa Clara CA 95051). From this qualified RNA sample, a normalized cDNA library (insert size: 300-400 bp) was constructed by a process with TrueSeq RNA Sample Prep Kit (Illumina, San Diego, CA 92122) followed by Duplex Specific Nuclease normalization (Illumina) according to the manufacturer’s instruction. Paired-end sequencing (100 bp read x 2) was carried out by Illumina HiSeq2000 and a set of raw read data was produced after base calling and Chastity filtering (CASAVA ver.1.8.1., Illumina).