title: GSM3317488: HCT116_WT_WGBS; Homo sapiens; Bisulfite-Seq
library_construction_protocol: The DNA was extracted using Qiagen DNeasy Blood & Tissue Kits. After samples DNA testing, negative control DNAs were added into the initial DNA pool, and then fragmented into a 200-300bp range using Covaris S220. Next, terminal repairing, A-ligation, methylation sequencing adapters ligation were performed to DNA fragments. Then, DNA fragments undergone a bisulfite treatment using the EZ DNA Methylation Gold Kit from Zymo Research. Library concentration was firstly quantified by Qubit 2.0, and then is diluted to 1 ng/ul before an insert size verification using the Agilent Bioanalyzer 2100 and followed by Q-PCR. Finally, the library was paired-end sequenced using the Illumina HiSeq 4000.
attributes.tag: GEO Accession
attributes.value: GSM3317488