title: GSM2224571: Sox2 WT stomach 2 [RNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2224571