title: HiSeq X Ten sequencing; Amplicon sequencing of genomically-encoded guide RNA sequences for the Brunello CRISPR library in eHap cells
design: Amplicon sequencing of genomically-encoded guide RNA sequences for the Brunello CRISPR library in eHap cells
library_construction_protocol: eHap cells collected and either genomic DNA is immediately extracted or the cells are fixed, permeabilized, and then subjected to immunostaining flow cytometry for sorting. QIAmp DNA mini kit. PCR to attached adapters and illumina barcodes.
attributes.tag: Experimental Factor: phenotype
attributes.value: RPS6p stained