title: GSM2830428: H3K27ac ChIP-seq from Proliferating Fibroblasts, Replicate #2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP was performed as described in Shah et al., Genes & Development 2013 from ~12e6 cells. Briefly, cross-linked cells were lysed and the chromatin was sheared using a Covaris Ultrasonicator to achieve an average size of <500 bp. 2ug of sonicated chromatin (estimated by DNA concentration) was used per immunoprecipitation reaction, and 10% of the amount was saved as input. Immunoprecipitation was performed using protein A Dynabeads (Thermo Fisher). 1ng of DNA from immunoprecipitation and input was used to prepare libraries using the NEBNext Ultra II kit (New England Biolabs). The libraries were checked for quality and quantity by Bioanalyzer (Agilent) and qPCR (Kapa Biosystems) respectively. Multiplexed libraries were sequenced on a single lane of the NextSeq 500 platform (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2830428