title: Illumina HiSeq 2000 paired end sequencing; Expression/Transcription profiling by high throughput sequencing of TCF7L2 KD and control in INS1 832/13 cells
design: Expression/Transcription profiling by high throughput sequencing of TCF7L2 KD and control in INS1 832/13 cells
library_construction_protocol: 60uM siRNA against Tcf7l2 (rat), 48 hours incubation- Human Molecular Genetics, 2011 doi:10.1093/hmg/ddr454 Library preparation was preformed according to mRNA Sequencing Sample Preparation Guide (Illumina, TruSeq RNA sample preparation kit). Reagents were taken from the Illumina sample preparation kit (Illumina, TruSeq RNA sample prep kit). Illumina TrueSeqTM RNA Library preparation protocol, paired-end
attributes.tag: Experimental Factor: RNA interference
attributes.value: siRNA against Tcf7l2
attributes.tag: Experimental Factor: genotype
attributes.value: TCF7L2 knock down