title: GSM2747120: MBT RNA-seq rep2; Danio rerio; RNA-Seq
library_construction_protocol: RNA was extracted from dechorinated and deyolked zebrafish embryos (4 hpf (MBT) and 5.3 hpf (post-MBT) ) using the Norgen Animal and Tissue RNA Extraction Kit (#25700) according to the manufacturer's instructions including the optional step of DNase I treatment. 5ng of purified, total RNA was used to produce RNAseq libraries for each sample using the EpiCentre TotalScript™ RNA-Seq Kit (#TSRNA12924). Briefly, total RNA was reverse-transcribed using random hexamer primers, followed by second-strand synthesis and adapter addition via tagmentation. The final RNA-Seq library was amplified by PCR for a total of 10 cycles.
attributes.tag: GEO Accession
attributes.value: GSM2747120