title: GSM2064484: mRNA_Zli_E10.5; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq. The 429M20eGFP BAC reporter line48 was used to guide the dissection of Shh expressing cells from the ventral midbrain, ventroposterior diencephalon and zli of E10.5 embryos under a fluorescent stereomicroscope. Total RNA was extracted from GFP+ brain tissue isolated from approximately 30 embryos using the miRNeasy Micro Kit (Qiagen, Valencia, CA). The RNA-seq library was prepared from 1µg of total RNA according to the manufacturer's protocol for TruSeq RNA Sample Prep Kits (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2064484