title: GSM3316358: Pooled Serum 2 from Health Chinese Subjects; Homo sapiens; miRNA-Seq
library_construction_protocol: 8 pooled samples (each pooled from 10 healthy Chinese subjects, mean age 50.9 ± 7.9) was conducted in the Healthy Physical Examination Center of the Jinling Hospital. The health condition checkup included detailed history, physical, radiological examinations, blood tests, and abdominal sonography. Subjects who showed no abnormalities during the medical checkup were enrolled. Written informed consent was obtained from all donors prior to the study, and the study was approved by the ethics committee of Nanjing University, China. Venous blood samples (∼5 ml) were collected from each donor and placed in a serum separator tube. Samples were processed within 1 h. Separation of the serum was accomplished by centrifugation at 800× g for 10 min at room temperature, followed by a 15 min high-speed centrifugation at 10 000× g at room temperature to completely remove the cell debris. The supernatant serum was recovered and stored at −80 °C until analysis.  Total RNA was extracted from 100 ml of serum using the Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3316358