title: RNA-Seq of Doryteuthis pealeii: adult male giant axon
design: To compare transcriptome-wide RNA editing between axons and cell bodies, four adult male specimens of the squid Doryteuthis pealeii were collected from the Marine Biological Laboratory in Woods Hole, Massachusetts. The giant fiber lobe(GFL) and giant axon (GA) tissues were manually dissected.Following dissection, axoplasm was extruded from the GA sample and total RNA was extracted from both axoplasm and GFL samples using Trizol. The RNA-Seq libraries for all the samples were prepared using the TruSeq Stranded mRNA Sample Prep Kit and were sequenced by Illumina sequencing using two lanes of the Illumina HiSeq 4000 instrument.