title: GSM1404653: input plus acyclovir; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin was fixed by application of 1% (v/v) formaldehyde for 15 min  then sonnicated on ice (10 x 10s-pulses; 30% amplitude output on a Branson model 250 Microtip at setting 5 (Sonics Vibacell). The chromatin was pre-cleared with protein A/G-sepharose bead slurry (Sigma) that had been pre-blocked in 0.5% (w/v) fraction V BSA (Sigma) in DPBS. 2% (v/v) of the pre-cleared extract was retained as the input control sample while the remainder was incubated with 10μg of Zta-specific goat antibody (Santa Cruz # sc-17503) or control goat IgG for 1 h at 4oC]. The input control sample and the precipitated DNA were sequentially treated with 0.2μg/ml RNAse A and 0.2μg/ml Proteinase K and the DNA purified. Sequencing libraries were prepared using 10ng of the input and ChIP DNA using a ChIP-Seq sample preparation kit (Illumina) following the manufacturer’s protocol, except that the library (150-350bp fragments) was purified from the gel after PCR-amplification. The DNA fragments were separated on a 2% agarose gel in TAE buffer, visualized using SYBR-safe stain and 150-300bp fragments were excised and purified using a gel extraction kit (Qiagen).
attributes.tag: GEO Accession
attributes.value: GSM1404653