title: RNAseq of V. anguillarum RV22 strain grown in CM9 medium at 25 C under low-iron (sample 2)
design: V. anguillarum RV22 was grown aerobically in CM9 minimal medium under iron-deficient condition (50 M 2,2-dipyridyl) at two different temperatures: 25 C and 15 C. As reference controls, V. anguillarum was grown in CM9 supplemented with iron (10 M Fe2SO4) at 25 C. Total RNA was extracted with RNAwiz (Ambion) from triplicate biological samples and used to construct independent cDNA libraries. Sequencing (ca. 20 M of 2x150 bp reads) was performed in an Illumina MiSeq sequencing machine using NextSeq High Output 1x150pb kit.