title: GSM3990118: 7.LE-16We-m1-S7-all-R1-001; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA from the fat tissue of three cows per group was extracted using a RNeasy Midi kit (Qiagen®, Courtaboeuf, France) and purified using a DNAfree kit (Invitrogen by Life Technologies), according to the manufacturer's recommendations. Directional RNA-Seq libraries were constructed using the TruSeq Stranded Total RNA library prep kit (Illumina), following the manufacturer's instructions, using 750 ng of total RNA.