title: GSM2831577: C3 M rep4 ; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from fresh liver tissues, with 5 biological replicates per group. The left lateral liver lobe was rapidly removed and homogenized in Trizol (Thermo Fisher Scientific), and phase separation was performed according to the manufacturer's instructions. The aqueous phase was then purified using Acid Phenol:Chloroform (Ambion). Following, equal volume of 50% ethanol was added to the aqueous layer and then purified using the RNeasy Mini Kit (Qiagen) with RNase-Free DNase treatment (Qiagen). Indexed paired end libraries were constructed for each total RNA sample using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina), according to the manufacturer's instructions. Libraries were randomly distributed to three pools, and each pool was sequenced using the Illumina HiSeq 2500 Sequencing System, run as indexed 125 base paired end reads, at the NIH Intramural Sequencing Center.
attributes.tag: GEO Accession
attributes.value: GSM2831577