title: Illumina MiSeq paired end sequencing
design: 149 airway samples from adults sequenced to characterize bacterial microbiome in allergy and asthma.
library_construction_protocol: DNA was extracted using lysing matrix E tubes and AllPrep kit (Qiagen) protocol; V4 region of 16S rRNA amplified with 515F and 806R; sequenced on Illumina MiSeq