title: GSM1706457: second round replicate 3; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from the surviving cells using DNeasy Blood and Tissue Kit (QIAGEN) The library construction follows Illumina’s TruSeq method. Briefly, sgRNA sequences were amplified by nested PCR with the first pair of primers, GATAGGCTTGGATTTCTATAAG (forward) and CTGCTGGAATCTCGTGAAG (reverse), followed by amplification with the second pair of primers, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNCTTGGAGAAAAGCCTTGTT (forward) and CAAGCAGAAGACGGCATACGAGATXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCGCTATTTCTAGCTCTAAAAC (reverse, where XXXXXX indicates index sequences). The indexes for first round screen are: replicate1: CAGATC; replicate2: TGCTCA; replicate3: CAGTAG. For the second round screen, the indexes are: replicate1: GCTGAT; replicate2: CTGAGT; replicate3: TCGACT.
attributes.tag: GEO Accession
attributes.value: GSM1706457