title: GSM2830488: T1D-2; Homo sapiens; RNA-Seq
library_construction_protocol: RNAqueous micro-scale phenol-free total RNA isolation kit RNA concentration and integrity was evaluated (Qubit Fluormeter; Invitrogen, Carlsbad, CA; Agilent 2100 Bioanayzer), and high-integrity total RNA was amplified (Ovation system; NuGen Technologies, San Carlos, CA) per standard protocol as described previously (Brissova et al., 2014). Amplified cDNA was sheared to target 200bp fragment size and libraries were prepared using NEBNext DNA Library Prep (New England BioLabs, Ipswich, MA). Accurate quantification of libraries for sequencing was determined using the qPCR-based KAPA Biosystems Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). 50bp Paired End (PE) sequencing was performed on an Illumina HiSeq 2500(Illumina Inc., San Diego, CA, USA)  to generate approximately 50 million reads per sample
attributes.tag: GEO Accession
attributes.value: GSM2830488