title: GSM4221655: U2OS_DNase-seq; Homo sapiens; DNase-Hypersensitivity
library_construction_protocol: Nuclei were extracted from cells and incubated with limiting concentrations of the DNA endonuclease DNase I (Sigma) supplemented with Ca2+ and Mg2+ for 3 min at 37 °C. DNA fragments isolated from DNaseI digestions were end-repaired, A-tailed, and then Illumina adapters were ligated during an overnight digestion at 16C. Resulting libraries were PCR amplified for 8-10 cycles.
attributes.tag: GEO Accession
attributes.value: GSM4221655