title: RIP-seq of homo spaiensfemale
design: human CELF6 fragments were subcloned into a modified lentiviral vector (PCDH-CMV-MCS-EF1A-PURO-T2A -EGFP) with EGFP and puromycin selection markers. All of the constructs generated were confirmed by DNA sequencing. Lentiviral packaging plasmids were co-transfected with the backbone plasmid into 293T cells for virus production. Hela Cells were selected in 4 g/mL puromycin in the culture medium to generate the stable transfections.