title: GSM2407404: Sample_stage 1-repeat 1; Citrus limon; RNA-Seq
library_construction_protocol: Total RNA was isolated from bud (three biological replicates per genotype) using the Plant RNAiso Plus according to the manufacturer’s instructions (Takara, Kusatsu, Japan). Genomic DNA was removed from total RNA by DNase treatment (Promega, Madison, WI, USA). All RNA samples were quantified and examined for protein contamination and reagent contamination by a Nanodrop ND 1000 spectrophotometer (NanoDrop, Wilmington, DE, USA). Total RNA were sent to Beijing Genomics Institute (Shenzhen, China) where the libraries were produced and sequenced using Illumina’s Genome Analyzer (Solexa). The sequencing and data analysis was carried out essentially as in previous studies (Mortazavi et al. 2008). In this study, probability ≥ 0.8 and the absolute value of log2Ratio ≥ 1 were used as the threshold to judge the significance of gene expression difference (Tarazona et al. 2011).
attributes.tag: GEO Accession
attributes.value: GSM2407404