title: WE2- Whole Embryo Transcriptome (Gallus gallus –HH10)
design: Sorted cells were pelleted, washed with PBS, and immediately lysed with Lysis Buffer. RNA was extracted with RNAqueous-Micro Kit 390 (Ambion AM1931); genomic DNA was removed by 30 min of TURBO DNase treatment. Quality of the RNA was assayed in an Agilent 2100 Bioanalyzer. RNA was subsequently amplified with the Ovation RNA-Seq System V2, yielding double-stranded cDNA that was used for library construction. Sequencing libraries were built according to Illumina Standard Protocols. SR50 sequencing was performed in a HiSeq Illumina machine.