title: GSM3384768: GBM47 [RNA-Seq]; Homo sapiens; RNA-Seq
library_construction_protocol: Glioblastoma tissue was isolated from patients and flash frozen in liquid nitrogen followed by pulverization in a mortar and pestle. RNA was isolated using the Qiagen MiRNeasy kit according to manufacturers recommendations. Dnase treatment was performed on the samples. Quality control and quantity of RNA was assessed on the Agilent Tape Station. RNA was prepared accoding to Illumina manufacturers conditions using a strand-specific RNA library preparation with ribosomal RNA depletion. Total RNA was sequenced on a Illumina Hi-Seq in a 2x150bp configuration.
attributes.tag: GEO Accession
attributes.value: GSM3384768