title: GSM2752816: RNA-seq_Control_rep1 (ERCC spike-in); Mus musculus; RNA-Seq
library_construction_protocol: Forty-eight hours (48h) after transfection, puromycin (2 µg /ml) selection was applied for an additional 24h period, before cell collection and RNA preparation. Total RNA was obtained via phenol-chloroform extraction (QIAzol Lysis Reagent – QIAGEN) followed by purification via Quick-RNA™ MicroPrep (Zymo Research). ERCC spike-in controls were added according to the manufacturer instructions
attributes.tag: GEO Accession
attributes.value: GSM2752816