title: GSM1704713: M_Pod_Control_H3K27me3 Chip-seq_3; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were processed using Magna ChIP™ A - Chromatin Immunoprecipitation Kit, Millipore,Cat No. 7-408, Lot No.2500615 to obtain the Chip DNA using H3K27me3 antibody Libraries from the immunoprecipitated and whole cell lysate DNA were prepared using the NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina (NEB, Cat. No. E6200S).  In brief, immunoprecipitated DNA was first end-repaired, followed by a dA-Tailing of End Repaired DNA using NEBNext dA-Tailing reaction mixture (NEB, Cat No. E6053), and ligated to custom adapters with NEBNext adaptor (NEB E7500) having Oligos for Illumina. Fragments of 150 to 700 (+120 bp) bp of adaptor-ligated DNA were size-selected using AMPure XP Beads followed by ligation-mediated PCR amplification of the adaptor-ligated DNA using Phusion DNApolymerase.
attributes.tag: GEO Accession
attributes.value: GSM1704713