title: DNA-seq of Zea mays: diploid, non-glossy leaf
design: 12 ng/l of purified leaf DNA was sonicated in a 100 l volume with a Diagenode Bioruptor for seven minutes on high setting with 30-second on-off intervals, yielding fragments averaging about 500 bp in length. DNA sequencing libraries were prepared using the KAPA Hyperprep Kit (KK8502) with KAPA single-indexed adapters (KK8700). 600 ng sonicated DNA was used as input for each, and 3 cycles of PCR were used to amplify libraries.