title: GSM4066608: Line 1, Ground rep1 [GB1]; Homo sapiens; RNA-Seq
library_construction_protocol: RNALater (Thermo Fisher) was used to preserve flight samples designated for downstream RNA-sequencing analysis. Samples preserved in RNALater were stored at 4°C until return to our laboratory, when RNA was isolated from preserved wells in the BioCells using RNeasy kit (Qiagen) following the manufacturer's protocol. DNase treatment was performed using RNase-free DNase kit (Qiagen). All RNA samples had RNA integrity number ≥ 8.9 and concentration ≥ 48 ng/µL (Agilent 2100 Bioanalyzer). Library prep and sequencing were performed by BGI with 30-40 million paired-end 100bp reads per sample on the Illumina HiSeq platform.
attributes.tag: GEO Accession
attributes.value: GSM4066608