title: "Multiplex p53 cDNA PacBio, sample 888Bx1"
design: "p53 cDNA was amplified by RT-PCR from tumour total RNA. The resulting 1 kb PCR product was split into two fragments (N-terminal and C-terminal) by a PCR reaction that added 454 priming sites and multiplexing tags. 96 fragments were pooled, corresponding to 48 biopsies. Each fragment had identical tags at each end, but each fragment had a different tag. The primers have names like A39p53f159, where 39 is the Roche MID Tag name, 159 is the last nucleotide in the primer relative to the p53 ORF, and A/f mean p53 forward strand. This generates fastq names like 158_Bx1_A39P53F159, meaning tumour 158, biopsy 1, tag 39, sense primer ending at p53 ORF nt 159."