title: RNA-Seq of Mus caroli: pooled male and female adult vomeronasal organ
design: VNO epithelia were dissected from at least one male and one female from each species and subsequently pooled to obtain V1R repertoires unbiased to a particular sex. Total RNA was extracted from VNO tissues using the Qiagen RNeasy kit, and subsequently quantified using QuBit Fluorometric Quantitation. RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used for RNA Isolation. Sequences were indexed using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, NEB #E7600). VNO libraries were sequenced as 300 bp paired-end reads on Illumina MiSeq platform through the Biotechnology Resource Center (Institute of Biotechnology) at Cornell University