title: Ruminococcoides bili genome and assembly
design: Genomic DNA from IPLA60002 was purified using the DNeasy blood and tissue kit (Qiagen, Germany) and genome sequencing were determined using a 250-290 paired-end libraries with Illumina MiSeq Sequencing System (Illumina, USA) at GenProbio SRL (Parma, Italy). Genome assembly was performed with MIRA assembler v4.0.2. Open reading frames prediction was performed with Prodigal v2.6 (11). Automatic annotation of the ORFs was performed with BLAST against NCBI databases and HMMER software against PFAM database of the European Bioinformatics Institute (EMBL-EBI). Improved quality of the final contigs was performed with Burrows-Wheeler Aligner, SAMtools suite and VarScan v2.2.3 software packages.