title: Genome wide miRNA sequencing of SH-SY5Y cells overexpressing mutant MIR204 (clone 5)
design: SH-SY5Y cells stably overexpressing MIR204 with rs7861254 genotype TT (generated via lentiviral transduction). The paternal transduced cell line was split and clones were cultured separately to give biological replicates. Sequenced library: MIR204SNP cells, clone 5.  Libraries were prepared using the TruSeq small RNA library kit (Illumina). PCR products were separated on a Pippin Prep system (Sage Science) to recover the 147 nt and 157 nt fractions containing mature miRNAs. Small RNA libraries were sequenced on a NextSeq 500 (Illumina) using single-end sequencing with read length of 75 bp. Library preparation and sequencing was performed at Biogazelle N.V. (Belgium).