title: PacBio 16S rRNA gene sequence data: Pooled sample: Sample V1V9_october
design: " ""There are 4 samples present in the PacBio Run and the V1V9 region from each sample was sequenced in triplicate. The data contains a human gun metagenome, a mouse gut metagenome, a soil metagenome, and a synthetic metagenome. In order to demultiplex the data you will need to use the following primers (AGRGTTTGATYMTGGCTCAG GGYTACCTTGTTACGACTT) and the sample and barcode information. mock1 (TGTGTATCAGTACATG CATGTACTGATACACA), mock2 (ACACGCATGACACACT AGTGTGTCATGCGTGT), mock3 (GATCTCTACTATATGC GCATATAGTAGAGATC), mouse1 (ACAGTCTATACTGCTG CAGCAGTATAGACTGT), mouse2 (ACAGTCTATACTGCTG CAGCAGTATAGACTGT), mouse3 (CTGCGTGCTCTACGAC GTCGTAGAGCACGCAG), human1 (TCAGACGATGCGTCAT ATGACGCATCGTCTGA), human2 (CTATACATGACTCTGC GCAGAGTCATGTATAG), human3 (TACTAGAGTAGCACTC GAGTGCTACTCTAGTA), soil1 (GCGCGATACGATGACT AGTCATCGTATCGCGC), soil2 (CGCGCTCAGCTGATCG CGATCAGCTGAGCGCG), soil3 (GCGCACGCACTACAGA TCTGTAGTGCGTGCGC). These data were generated in October 2015 and are from Run 0001. The P6C4 chemistry was used to generate these data."""