title: PROK RNA based amplicon 16S rRNA gene of a control SUP sample at day 0
design: cDNA sample obtained from RNA extract (MoBio PowerSoil RNA, High Capacity cDNA Reverse Transcription kit). Amplification of Hypervariable V4 region of prokaryotic 16SrRNA gene. 515F-806R primer set.  Dual-indexed Illumina compatible library in a single PCR step. Primary PCR with fused CS primersat their 5' ends. PCR products were normalized using Invitrogen SequalPrep DNA normalization plates.