title: GSM4222792: N701 WT1; Mus musculus; RNA-Seq
library_construction_protocol: Cell suspensions were generated as described in Ali et al. Cell Reports 2019, briefly: vaginae were dissected out, washed with Ca2+ and Mg2+ free Hanks Balanced Salt Solution (HBSS), and were slit open to expose the epithelium during subsequent enzymatic digestion. The tissues were placed in DMEM/F12 containing 1mg/ml pronase (Sigma) and 0.5mg/ml DNase1 (Sigma) at 4°C overnight with slow shaking. Enzymatic digestion was stopped by adding DMEM/F12 medium (Sigma) supplemented with 10% fetal bovine serum. The epithelium was then squeezed out with the help of a pair of forceps under the dissecting microscope. Following two successive washes in DMEM/F12, the single cell suspension was prepared using 40µm cell strainer before capture.
attributes.tag: GEO Accession
attributes.value: GSM4222792