title: Illumina HiSeq 2500 sequencing; Meningeal cells contribute to postnatal cortical neurogenesis.
design: Meningeal cells contribute to postnatal cortical neurogenesis.
library_construction_protocol: Subventricular zone (SVZ) tissue and meninges tissue was isolated from postnatal day 0 (P0) mice and processed for RNA preparation (SVZ-bulk and meninges-bulk, respectively). Prominin-positive cells were isolated from P0 SVZ tissue, grown to neurospheres in suspension culture (SVZ-prominin neurospheres) and used for RNA extraction. Similarly, PDGFRb-positive cells from meninges tissue were grown in suspension cultures to PDGFRb-neurospheres, which were then used for RNA extraction. RNA was extracted using TRIzol (Life Technologies).  Starting from total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries using the KAPA stranded mRNA-seq kit (Sopachem, Eke, Belgium).
attributes.tag: Experimental Factor: homogenized meningeal tissue
attributes.value: fraction