title: GSM2065677: Wild-type Villus Pol2 ChIP-Seq rep 1; Mus musculus; ChIP-Seq
library_construction_protocol: Epithelium from proximal 1/3 of mouse intestine was isolated by incubation with 5mM EDTA in PBS for 45 minutes at 4°C. Cells were snap-frozen in liquid nitrogen and pooled for subsequent ChIP analysis. Whole cell lysates were sonicated and incubated with 5 ug Pol2 antibody overnight (SC-899, Santa Cruz) for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
attributes.tag: GEO Accession
attributes.value: GSM2065677