title: GSM3316517: B3 tdTom- Ecs; Mus musculus; RNA-Seq
library_construction_protocol: PECAM1+ CD45- CD11b- KIT- ECs were isolated from n = 3 E12.5 Csf1r-Cre;RosatdTom embryos and divided into tdTom+ and tdTom- populations with the Influx cell sorter before RNA was extracted with the RNeasy Micro Kit (QIAGEN). cDNA was generated and amplified using the SMART-seq V4 ultra low input RNA kit (Clontech). 100 pg of amplified cDNA per sample was used to prepare a library with the Nextera XT kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3316517