title: Sample: p2255_d180_part1
design: The dataset contains T-cell Receptor Beta Chain sequences from patient p2255_and_p3602 at d180-part1_and_d180. Individual cDNA molecules were labeled with an unique molecular identifier (UMI) tag prior to library  preparation. Custom adapters were introduced via PCR. Both sides of the amplicons have sample-specific barcode. Libraries were prepared generally according to our standardized protocol (http://www.frontiersin.org/Journal/10.3389/fimmu.2013.00456/abstract) with some modifications (see the main publication). Pair-end 2x100 sequencing was performed using Illumina HiSeq2500 with custom sequencing primers. Sequences for sample p2255_d180 could be extracted from the file using the substring 'NNNNNTNGGTAGNTNNNNNNTCT' for read 1 (contains sample-specific barcode GGTAG and UMI dispalyed as 12xN). Sequences for the sample p3602_d180 could be extracted from the file using the substring 'NNNNTNNGCAAANNTNNNNNNTCT'  for read 1 (contains sample-specific barcode GCAAA and UMI dispalyed as 12xN)