title: GSM2222674: IMR32; Homo sapiens; ncRNA-Seq
library_construction_protocol: The cell lines are procured from NCCS, India and maintained in MEM (IMR 32) and Hams F12K (SH-SY-5Y) growth media supplemented with 10% FBS and 1% antibiotics Pen-Strep at proper growing conditions. Total RNA was extracted from the above cell lines with the use of RNeasy Mini kit (Qiagen, USA) according to the manufacturer’s instructions. The purity of isolated RNA was evaluated using NanoDrop spectrophotometer (Thermo Scientific) by measuring absorbance at 260 nm and 280 nm. An aliquot of each the samples isolated from IMR-32 and SH-SY-5Y was also run on an Agilent RNA Bioanalyzer chip to check for RNA integrity number (RIN) and was found to be 8.3 and 9.2 respectively. The RNA libraries were constructed for sequencing following Illumina TruSeq Small RNA library protocol outlined in “TruSeq Small RNA Sample Preparation Guide” (Part # 15004197; Rev. F; February 2014) and using TruSeq Small RNA sample preparation kit (Illumina, # RS-200-0012).
attributes.tag: GEO Accession
attributes.value: GSM2222674