title: Microbe
design: DNA was extracted from each 0.5 g (fresh weight) soil sample with the CTAB/SDS method, using MP FastDNASPIN Kits for soil (MP Biomedicals, Solon, OH, USA). After concentration and purification, extracted DNA was diluted to 1 ng/l using sterile water. We then amplified the ITS5-1737F (GGAAGTAAAAGTCGTAACAAGG) and ITS2-2043R (GCTGCGTTCTTCATCGATGC) for fungi