title: HuR iCLIP sequence data
design: HeLa cells were plated on 100mm dishes and crosslinked when semi-confluent in 6mL of PBS with a Spectrolinker XL-1500 two times 100mJ/cm2.  Cells were scraped and transferred to tubes, pelleted and snap frozen. iCLIP was performed according to Konig et al (7). Samples were amplified, purified with Beckman Genomics Ampure XP beads, quantitated with Agilent Bioanalyzer and DNA 1000 Chip. Clusters were generated with the Illumina cBot station. Samples were sequenced on an Illumina GAIIx single read 2 X 36. CASAVA 1.6 was used to analyze and generate the raw reads.    Three replicates, barcodes are  5'- NNNGGCGNN  5'- NNNCCGGNN  5'- NNNAACCNN