title: GSM1399652: gSELEXx1; Arabidopsis thaliana; OTHER
library_construction_protocol: For the genomic SELEX experiment, Arabidopsis thaliana genomic DNA was extracted from wild type 3 week old plant leaves and fragmented to 100 bp using COVARIS. 2 μg of this DNA was processed for end repair and adaptor ligation following the manufacturer’s instructions (Illumina) and used as indicated above for incubation with purified GST-SUVR5 zinc finger domain protein. Two genomic SELEX experiments were performed, one using only one binding/eluting cycle (x1: control) and one with 9 cycles (x9). The recovered DNA was sequenced using an Illumina Genome Analyzer.
attributes.tag: GEO Accession
attributes.value: GSM1399652