title: Small RNA sequencing of schistosoma japonicum 14 dpi male worm
design: 14 dpi male S. japonicum total RNA was isolated using QiagenmiRNeasy Mini Kit (Valencia, CA) according to the manufacturers instruction.  10 g total RNA was separated by electrophoresis on a Novex 15% TBE-Urea gel (Invitrogen Co. Ltd) and RNA in length of 18-30 nt was purified and ligated to 3 and 5 adapters. Then for reverse transcription, we used a RT-PCR kit (Invitrogen Co. Ltd) to produce complementary DNA (cDNA). Finally, the cDNA was amplified using adapter primers, the products were purified using a 6% TBE PAGE gel (Invitrogen Co. Ltd) and solexa sequencing.