title: GSM4066590: 862: Lhx5-EGFP-Rpl10a-DT154 Hypothalamus IP Rep1; Mus musculus; RNA-Seq
library_construction_protocol: The hypothalamus was dissected and homogenized and tagged polysomes were immunopurified with monoclonal anti-EGFP antibodies bound to biotinylated-Protein L (Thermo Fisher) coated streptavidin-conjugated magnetic beads (Life Technologies). Total RNA from IPs was purified with the Agilent Nanoprep kit following manufacturer's protocol. Ribosomal-bound RNA was extracted and treated as described in PMID 24810037, Heiman M, Kulicke R, Fenster RJ, Greengard P, Heintz N. (2014) Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nat Protoc. 9:1282-1291
attributes.tag: GEO Accession
attributes.value: GSM4066590