title: GSM2831046: CTR_1_heart; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from mice heart samples (4 wt, 4 HFD and 4 HFD + AA6) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. For next generation sequencing (NGS) RNA was isolated from mice heart samples (4 wt, 4 HFD and 4 HFD + AA6) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and libraries integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). 1 µg total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry.
attributes.tag: GEO Accession
attributes.value: GSM2831046