title: 16S V1-V3 rRNA genes of human vaginal microbiome: premenarcheal adolescent vagina: Sample 12001Vag1
design: "Total genomic DNA was extracted and 16S rRNA genes were amplified using primers flanking V1 and V3 regions. Primers (modified 27F, 534R) are described by Frank JA, Reich CI, Sharma S, et al. (2008) Appl Environ Microbiol 74:2461-2470."