title: NextSeq 500 paired end sequencing; RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells (1)
design: RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells (1)
library_construction_protocol: Cells sorted and pooled using a FACS machine GNRH1 neurons were differentiated based a protocol previously published (Lund C et al., 2016, Stem cell reports) During the differentiation, after initial treatment with Dorsomporphin and SB431542, FGF8 treatment was continued with DAPT treatment in the terminal step Using RNAqueous micro Total RNA Isolation kit (Thermo Scietific) RNA was isolated. RNA was analyzed for concentration, integrity and quality using Qubit Fluorometer and TapeStation 4200 (Agilent). NEBNext Ultra Directional polyA capture method as paired-end sequencing for read length 75 bp
attributes.tag: Experimental Factor: stimulus
attributes.value: untreated
attributes.tag: Experimental Factor: time
attributes.value: 20