title: Apis mellifera intermissa full genome sequence
design: ~5 microgram of genomic DNA was made up to 50 ul with nuclease free water (Ambion) and sonicated using   covaris to obtain desired fragment length ranging between 150 to 600 bp. The resulting fragmented DNA was   cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). The size distribution was checked by   running an aliquot of the sample on Agilent High Sensitivity Bioanalyzer Chip.  Subsequently libraries for whole genome sequencing were constructed according to the TruSeq DNA library   protocol outlined in “TruSeq DNA Sample preparation guide”. DNA was   subjected to a series of enzymatic reactions that repair frayed ends, phosphorylate the fragments, and add a   single nucleotide A overhang and ligate adaptors (Illumina’s TruSeq DNA sample preparation kit). Sample   cleanup was done using Ampure SPRI beads. After ligation, ~300 – 500 bp was size selected on a 2% agarose   gel and cleaned using MinElute column (QIAGEN). PCR amplification was done and cleaned up using Ampure   SPRI beads. The prepared libraries were quantified using Nanodrop and validated for quality by running an   aliquot on High Sensitivity Bioanalyzer Chip