title: ESCC10_ShuhouPlasma
design: TruSight Cancer sequencing panel For preparing Illumina sequencing         library, 1 ug genomic DNA were sheared to fragments of 300~400bp by Covaris S2 (Covaris,         Germany), then end-repaired, A-tailed and ligated to Illumina sequencing adapters. The         ligated products were size selected of 400~500 bp on a 2% agarose gel and amplified by         LM-PCR.