title: PacBio DNA WGS of Phaseolus lunatus accession G27455.
design: The SMRTbell Template Prep Kit was used to build a Pacific biosicences sequencing library from 10ug of high quality DNA following standard guidelines. In brief, DNA was fragmented and sheared using the Covaris device. Purification was performed using AMPurePB magnetic pearls. DNA fragments over 3Kbp were selected and ends were repaired. Blunt ends were created on each end and hairpin adapters are ligated to each end. Finally, the template DNA was purified and primers were annealed and the polymerase is bound to the annealed templates for sequencing in the PacBio Sequel instrument. Pacbio BAM files were converted to fastq format using the samtools fastq command