title: Illumina MiSeq sequencing; qiita_ptid_1935:10585.SBS1DS
design: We evaluated multiple conditions including Igf1r inhibition in proximal and distal segments after intestinal resection to evaluate the epithelial effects of the absence of mechanoluminal stimulation.SBS was created in adult male zebrafish by performing proximal stoma to distal intestinal ligation. These were compared to sham zebrafish.
library_construction_protocol: V4 Illumina MiSeq, 151f/806rbc
attributes.tag: barcode
attributes.value: TACTCTCTTAGC
attributes.tag: center_name
attributes.value: CHLA
attributes.tag: linker
attributes.value: GT
attributes.tag: mastermix_id
attributes.value: 14509333
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: primer_date
attributes.value: 22114
attributes.tag: primer_plate
attributes.value: 5
attributes.tag: processing_robot
attributes.value: JERE
attributes.tag: run_date
attributes.value: 5/6/13
attributes.tag: sample_center
attributes.value: CHLA
attributes.tag: sample_plate
attributes.value: CHLA_Plate1
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: tm300_8_tool
attributes.value: 111569B
attributes.tag: tm50_8_tool
attributes.value: 311426B
attributes.tag: water_lot
attributes.value: RNBC2791
attributes.tag: well_description
attributes.value: CHLA_Plate1_BQ_G8
attributes.tag: well_id
attributes.value: G8