title: RNA-seq of Homo sapiens: FLS: Sample 19
design: "Total RNA was extracted from synovial fibroblasts derived from two different RA patients using the RNeasy mini kit (Qiagen). RNA-sequencing libraries were generated from two patient donor FLS using Tru-Seq kits (Illumina) for poly-A selected transcripts. Single end sequencing was performed (Illumina HiSeq 2500, 50 bp read lengths). "