title: Enveronmental DNA sequencing
design: PCR amplification of domains from each library:  Degenerate primers were selected to amplify conserved regions in nonribosomal peptide synthetase (NRPS) adenylation domain (AD), type I polyketide synthase (PKSI) ketosynthase domain (KS) and type II polyketide synthases (PKSII) ketosynthase alpha (KSa).  Adenylation domains fragments (795 bp) were PCR amplified using primers AD-F (5’-GCSTACSYSATSTACACSTCSGG) and AD-R (5’-SASGTCVCCSGTSCGGTA) (12??).  KSa gene fragments (672 bp) were amplified using primers KSa-F (5’-TSGCSTGCTTCGAYGCSATC) and KSa-R (5’-TGGAANCCGCCGAABCCGCT) (7??).  KS domain fragments (760 bp) were amplified using primers: PKS-F (5’-GCIATGGAYCCICARCARMGIVT) and PKS-R (5’- GTICCIGTICCRTGISCYTCIAC) (13??).  In all the cases the forward primer incorporated a 454-sequencing adapter A (5’-CGTATCGCCTCCCTCGCGCCATCAG) along with appropriate tag oligomers to differentiate sequences derived from different environmental samples.  16S rRNA hypervariable regions (302bp) were amplified using primers 16S-F (5’-AYTGGGYDTAAAGNG) and 16S-R (5’-TACNVGGGTATCTAATCC).(S3)  Each reverse primer incorporated a 454-sequencing adapter B (5’-CTATGCGCCTTGCCAGCCCGCTCAG).  In some instances a 10 bp sample specific tag between the degenerate primer and the 454-sequencing adapter.    For AD, KSa and 16S genes, amplification reactions were carried out using two distinct PCR conditions in an attempt to amplify the most diverse set of eDNA gene sequences.  The first reaction mix (20 µl/reaction) contained 100 ng cosmid DNA, 0.5 uM each primer, 200 µM each dNTP, 1x Phusion GC buffer (New England Biolabs), 0.2 U Phusion polymerase (New England Biolabs) and 3% DMSO.  The second reaction mix contained 1x G buffer (Epicentre), 50 pmol of each primer, 2.5 U Taq polymerase (New England Biolabs) and 100 ng cosmid DNA.   For amplification with the AD and KSa primers with the first reaction mix followed a PCR protocol of 30 cycles consisting of 10 sec at 98 °C, 30 sec at 70 °C, and 30 sec at 72 °C, followed by a final extension at 72 °C for 5 min and the second reaction mix followed a PCR protocol of 35 cycles consisting of 1 min at 95 °C, 3 min at 72 °C, followed by a final extension at 72 °C for 5 min.  For amplifications with the 16S primers the first reaction mix followed a PCR protocol of 25 cycles consisting of 10 sec at 98oC, 30 sec at 55oC, and 30 sec at 72 °C, followed by extension at 72oC for 5 min. The second reaction mix followed a PCR protocol of 35 cycles consisting of 40 sec at 95 °C, 40 sec at 55 °C, 40 sec at 72 °C, followed by extension step at 72 °C for 5 min.  PCR reactions (20 µl) using KS domain specific reactions contained 1x G buffer, 50 pmol of each primer, 2.5 U Taq polymerase and 100 ng cosmid DNA.  The amplification reactions performed for 35 cycles consisting of 40 sec at 95 °C, 40 sec at 50 °C, 75 sec at 72 °C, followed by a final extension step at 72 °C for 5 min.        SFF file association with the corresoinding gene sequence   AB_Lib16s AZ_Lib16s UT_lib16s AB_AD AZ_AD UT_AD AB_KS AZ_KS UT_KS AB_KSa AZ_KSa UT_KSa  GDU8V6B01 * * * * * *    * * *  GPJCU3H01   * * * * * * * * * *  GPJCU3H02 *  * * * * * * * * * *  GPJCU3H03 *  * * * * * * * * * *  GPJCU3H04 * * *   *        GSISCEJ03 * * *  * *        GSISCEJ06 *   * * * * * * * * *  GSISCEJ07 *   * * * * * * * * *  GSISCEJ08    * * * * * * * * *    AB_library_16S  ==============  AACCAACC    UT_library_16S  ==============  AACCATCG    AB_Adenylation Domain  =====================  AB_ad_AB1       AACCATGC  AB_ad_AB2       AACCGCAT  AB_ad_AB3       AACCGCTA  AB_ad_AB4       AACCGGAA  AB_ad_AB5       AACCGGTT  AB_ad_AB6       AACCTACG  AB_ad_AB7       AACCTAGC  AB_ad_AB8       AACCTTCC  AB_ad_AB9       AACCTTGG  AB_ad_AB10      AACGAACG  AB_ad_AB11      AACGAAGC  AB_ad_AB12      AACGATCC  AB_ad_AB13      AACGATGG  AB_ad_AB14      AACGCCAT  AB_ad_AB15      AACGCCTA  AB_ad_AB16      AACGCGAA  AB_ad_AB17      AACGCGTT  AB_ad_AB18      AACGGCAA  AB_ad_AB19      AACGGCTT  AB_ad_AB20      AACGTACC  AB_ad_AB21      AACGTAGG  AB_ad_AB22      AACGTTCG  AB_ad_AB23      AACGTTGC  AB_ad_AB24      AAGCAACG  AB_ad_AB25      AAGCAAGC  AB_ad_AB26      AAGCATCC  AB_ad_AB27      AAGCATGG  AB_ad_AB28      AAGCCGAA  AB_ad_AB29      AAGCCGTT  AB_ad_AB30      AAGCGCAA  AB_ad_AB31      AAGCGCTT  AB_ad_AB32      AAGCGGAT  AB_ad_AB33      AAGCGGTA  AB_ad_AB34      AAGCTACC  AB_ad_AB35      AAGCTAGG  AB_ad_AB36      AAGCTTCG  AB_ad_AB37      AAGCTTGC  AB_ad_AB38      AAGGAACC  AB_ad_AB39      AAGGAAGG  AB_ad_AB40      AAGGATCG  AB_ad_AB41      AAGGATGC  AB_ad_AB42      AAGGCCAA  AB_ad_AB43      AAGGCCTT  AB_ad_AB44      AAGGCGAT  AB_ad_AB45      AAGGCGTA  AB_ad_AB46      AAGGTACG  AB_ad_AB47      AAGGTAGC  AB_ad_AB48      AAGGTTCC  AB_ad_AB49      AAGGTTGG  AB_ad_AB50      AATACCGC  AB_ad_AB51      AATACGCC  AB_ad_AB52      AATAGCGG  AB_ad_AB53      AATAGGCG  AB_ad_AB54      AATTCCGG  AB_ad_AB55      AATTCGCG  AB_ad_AB56      AATTCGGC  AB_ad_AB57      AATTGCCG  AB_ad_AB58      AATTGCGC  AB_ad_AB59      AATTGGCC  AB_ad_AB60      ACACACAC  AB_ad_AB61      ACACACTG  AB_ad_AB62      ACACAGAG  AB_ad_AB63      ACACAGTC  AB_ad_AB64      ACACCACA  AB_ad_AB65      ACACCAGT  AB_ad_AB66      ACACCTCT  AB_ad_AB67      ACACCTGA  AB_ad_AB68      ACACGACT  AB_ad_AB69      ACACGAGA  AB_ad_AB70      ACACGTCA  AB_ad_AB71      ACACGTGT  AB_ad_AB72      ACACTCAG  AB_ad_AB73      ACACTCTC  AB_ad_AB74      ACACTGAC  AB_ad_AB75      ACACTGTG  AB_ad_AB76      ACAGACAG  AB_ad_AB77      ACAGACTC  AB_ad_AB78      ACAGAGAC  AB_ad_AB79      ACAGAGTG  AB_ad_AB80      ACAGCACT  AB_ad_AB81      ACAGCAGA  AB_ad_AB82      ACAGCTCA  AB_ad_AB83      ACAGCTGT  AB_ad_AB84      ACAGGACA  AB_ad_AB85      ACAGGAGT  AB_ad_AB86      ACAGGTCT  AB_ad_AB87      ACAGGTGA  AB_ad_AB88      ACAGTCAC  AB_ad_AB89      ACAGTCTG  AB_ad_AB90      ACAGTGAG  AB_ad_AB91      ACAGTGTC  AB_ad_AB92      ACCAACCA  AB_ad_AB93      ACCAACGT  AB_ad_AB94      ACCAAGCT  AB_ad_AB95      ACCAAGGA  AB_ad_AB96      ACCACAAC  AB_ad_AB97      ACCACATG  AB_ad_AB98      ACCACTAG  AB_ad_AB99      ACCACTTC  AB_ad_AB100     ACCAGAAG  AB_ad_AB101     ACCAGATC  AB_ad_AB102     ACCAGTAC  AB_ad_AB103     ACCAGTTG  AB_ad_AB104     ACCATCCT  AB_ad_AB105     ACCATCGA  AB_ad_AB106     ACCATGCA  AB_ad_AB107     ACCATGGT  AB_ad_AB108     ACCTACCT  AB_ad_AB109     ACCTACGA  AB_ad_AB110     ACCTAGCA  AB_ad_AB111     ACCTAGGT  AB_ad_AB112     ACCTCAAG  AB_ad_AB113     ACCTCATC  AB_ad_AB114     ACCTCTAC  AB_ad_AB115     ACCTCTTG  AB_ad_AB116     ACCTGAAC  AB_ad_AB117     ACCTGATG  AB_ad_AB118     ACCTGTAG  AB_ad_AB119     ACCTGTTC  AB_ad_AB120     ACCTTCCA  AB_ad_AB121     ACCTTCGT  AB_ad_AB122     ACCTTGCT  AB_ad_AB123     ACCTTGGA  AB_ad_AB124     ACGAACCT  AB_ad_AB125     ACGAACGA  AB_ad_AB126     ACGAAGCA  AB_ad_AB127     ACGAAGGT  AB_ad_AB128     ACGACAAG  AB_ad_AB129     ACGACATC  AB_ad_AB130     ACGACTAC  AB_ad_AB131     ACGACTTG  AB_ad_AB132     ACGAGAAC  AB_ad_AB133     ACGAGATG  AB_ad_AB134     ACGAGTAG  AB_ad_AB135     ACGAGTTC  AB_ad_AB136     ACGATCCA  AB_ad_AB137     ACGATCGT  AB_ad_AB138     ACGATGCT  AB_ad_AB139     ACGATGGA  AB_ad_AB140     ACGTACCA  AB_ad_AB141     ACGTACGT  AB_ad_AB142     ACGTAGCT  AB_ad_AB143     ACGTAGGA  AB_ad_AB144     ACGTCAAC  AB_ad_AB145     ACGTCATG  AB_ad_AB146     ACGTCTAG  AB_ad_AB147     ACGTCTTC  AB_ad_AB148     ACGTGAAG  AB_ad_AB149     ACGTGATC  AB_ad_AB150     ACGTGTAC  AB_ad_AB151     ACGTGTTG  AB_ad_AB152     ACGTTCCT  AB_ad_AB153     ACGTTCGA  AB_ad_AB154     ACGTTGCA  AB_ad_AB155     ACGTTGGT  AB_ad_AB156     ACTCACAG  AB_ad_AB157     ACTCACTC  AB_ad_AB158     ACTCAGAC  AB_ad_AB159     ACTCAGTG  AB_ad_AB160     ACTCCACT  AB_ad_AB161     ACTCCAGA  AB_ad_AB162     ACTCCTCA  AB_ad_AB163     ACTCCTGT  AB_ad_AB164     ACTCGACA  AB_ad_AB165     ACTCGAGT  AB_ad_AB166     ACTCGTCT  AB_ad_AB167     ACTCGTGA  AB_ad_AB168     ACTCTCAC  AB_ad_AB169     ACTCTCTG  AB_ad_AB170     ACTCTGAG  AB_ad_AB171     ACTCTGTC  AB_ad_AB172     ACTGACAC  AB_ad_AB173     ACTGACTG  AB_ad_AB174     ACTGAGAG  AB_ad_AB175     ACTGAGTC  AB_ad_AB176     ACTGCACA  AB_ad_AB177     ACTGCAGT  AB_ad_AB178     ACTGCTCT  AB_ad_AB179     ACTGCTGA  AB_ad_AB180     ACTGGACT  AB_ad_AB181     ACTGGAGA  AB_ad_AB182     ACTGGTCA  AB_ad_AB183     ACTGGTGT  AB_ad_AB184     ACTGTCAG  AB_ad_AB185     ACTGTCTC  AB_ad_AB186     ACTGTGAC  AB_ad_AB187     ACTGTGTG  AB_ad_AB188     AGACACAG  AB_ad_AB189     AGACACTC  AB_ad_AB190     AGACAGAC  AB_ad_AB191     AGACAGTG  AB_ad_AB192     AGACCACT  AB_ad_AB193     AGACCAGA  AB_ad_AB194     AGACCTCA  AB_ad_AB195     AGACCTGT  AB_ad_AB196     AGACGACA  AB_ad_AB197     AGACGAGT  AB_ad_AB198     AGACGTCT  AB_ad_AB199     AGACGTGA  AB_ad_AB200     AGACTCAC  AB_ad_AB201     AGACTCTG  AB_ad_AB202     AGACTGAG  AB_ad_AB203     AGACTGTC  AB_ad_AB204     AGAGACAC  AB_ad_AB205     AGAGACTG  AB_ad_AB206     AGAGAGAG  AB_ad_AB207     AGAGAGTC  AB_ad_AB208     AGAGCACA  AB_ad_AB209     AGAGCAGT  AB_ad_AB210     AGAGCTCT  AB_ad_AB211     AGAGCTGA  AB_ad_AB212     AGAGGACT  AB_ad_AB213     AGAGGAGA  AB_ad_AB214     AGAGGTCA  AB_ad_AB215     AGAGGTGT  AB_ad_AB216     AGAGTCAG  AB_ad_AB217     AGAGTCTC  AB_ad_AB218     AGAGTGAC  AB_ad_AB219     AGAGTGTG  AB_ad_AB220     AGCAACCT  AB_ad_AB221     AGCAACGA  AB_ad_AB222     AGCAAGCA  AB_ad_AB223     AGCAAGGT  AB_ad_AB224     AGCACAAG  AB_ad_AB225     AGCACATC  AB_ad_AB226     AGCACTAC  AB_ad_AB227     AGCACTTG  AB_ad_AB228     AGCAGAAC  AB_ad_AB229     AGCAGATG  AB_ad_AB230     AGCAGTAG  AB_ad_AB231     AGCAGTTC  AB_ad_AB232     AGCATCCA  AB_ad_AB233     AGCATCGT  AB_ad_AB234     AGCATGCT  AB_ad_AB235     AGCATGGA  AB_ad_AB236     AGCTACCA  AB_ad_AB237     AGCTACGT  AB_ad_AB238     AGCTAGCT  AB_ad_AB239     AGCTAGGA  AB_ad_AB240     AGCTCAAC  AB_ad_AB241     AGCTCATG  AB_ad_AB242     AGCTCTAG  AB_ad_AB243     AGCTCTTC  AB_ad_AB244     AGCTGAAG  AB_ad_AB245     AGCTGATC  AB_ad_AB246     AGCTGTAC  AB_ad_AB247     AGCTGTTG  AB_ad_AB248     AGCTTCCT  AB_ad_AB249     AGCTTCGA  AB_ad_AB250     AGCTTGCA  AB_ad_AB251     AGCTTGGT  AB_ad_AB252     AGGAACCA  AB_ad_AB253     AGGAACGT  AB_ad_AB254     AGGAAGCT  AB_ad_AB255     AGGAAGGA  AB_ad_AB256     AGGACAAC  AB_ad_AB257     AGGACATG  AB_ad_AB258     AGGACTAG  AB_ad_AB259     AGGACTTC  AB_ad_AB260     AGGAGAAG  AB_ad_AB261     AGGAGATC  AB_ad_AB262     AGGAGTAC  AB_ad_AB263     AGGAGTTG  AB_ad_AB264     AGGATCCT  AB_ad_AB265     AGGATCGA  AB_ad_AB266     AGGATGCA  AB_ad_AB267     AGGATGGT  AB_ad_AB268     AGGTACCT  AB_ad_AB269     AGGTACGA  AB_ad_AB270     AGGTAGCA  AB_ad_AB271     AGGTAGGT  AB_ad_AB272     AGGTCAAG  AB_ad_AB273     AGGTCATC    AZ_Adenylation Domain  ========================  AZ_ad_ARZ1      AGGTCTAC  AZ_ad_ARZ2      AGGTCTTG  AZ_ad_ARZ3      AGGTGAAC  AZ_ad_ARZ4      AGGTGATG  AZ_ad_ARZ5      AGGTGTAG  AZ_ad_ARZ6      AGGTGTTC  AZ_ad_ARZ7      AGGTTCCA  AZ_ad_ARZ8      AGGTTCGT  AZ_ad_ARZ9      AGGTTGCT  AZ_ad_ARZ10     AGGTTGGA  AZ_ad_ARZ11     AGTCACAC  AZ_ad_ARZ12     AGTCACTG  AZ_ad_ARZ13     AGTCAGAG  AZ_ad_ARZ14     AGTCAGTC  AZ_ad_ARZ15     AGTCCACA  AZ_ad_ARZ16     AGTCCAGT  AZ_ad_ARZ17     AGTCCTCT  AZ_ad_ARZ18     AGTCCTGA  AZ_ad_ARZ19     AGTCGACT  AZ_ad_ARZ20     AGTCGAGA  AZ_ad_ARZ21     AGTCGTCA  AZ_ad_ARZ22     AGTCGTGT  AZ_ad_ARZ23     AGTCTCAG  AZ_ad_ARZ24     AGTCTCTC  AZ_ad_ARZ25     AGTCTGAC  AZ_ad_ARZ26     AGTCTGTG  AZ_ad_ARZ27     AGTGACAG  AZ_ad_ARZ28     AGTGACTC  AZ_ad_ARZ29     AGTGAGAC  AZ_ad_ARZ30     AGTGAGTG  AZ_ad_ARZ31     AGTGCACT  AZ_ad_ARZ32     AGTGCAGA  AZ_ad_ARZ33     AGTGCTCA  AZ_ad_ARZ34     AGTGCTGT  AZ_ad_ARZ35     AGTGGACA  AZ_ad_ARZ36     AGTGGAGT  AZ_ad_ARZ37     AGTGGTCT  AZ_ad_ARZ38     AGTGGTGA  AZ_ad_ARZ39     AGTGTCAC  AZ_ad_ARZ40     AGTGTCTG  AZ_ad_ARZ41     AGTGTGAG  AZ_ad_ARZ42     AGTGTGTC  AZ_ad_ARZ43     ATAACCGC  AZ_ad_ARZ44     ATAACGCC  AZ_ad_ARZ45     ATAAGCGG  AZ_ad_ARZ46     ATAAGGCG  AZ_ad_ARZ47     ATATCCGG  AZ_ad_ARZ48     ATATCGCG  AZ_ad_ARZ49     ATATCGGC  AZ_ad_ARZ50     ATATGCCG  AZ_ad_ARZ51     ATATGCGC  AZ_ad_ARZ52     ATATGGCC  AZ_ad_ARZ53     ATCCAACG  AZ_ad_ARZ54     ATCCAAGC  AZ_ad_ARZ55     ATCCATCC  AZ_ad_ARZ56     ATCCATGG  AZ_ad_ARZ57     ATCCGCAA  AZ_ad_ARZ58     ATCCGCTT  AZ_ad_ARZ59     ATCCGGAT  AZ_ad_ARZ60     ATCCGGTA  AZ_ad_ARZ61     ATCCTACC  AZ_ad_ARZ62     ATCCTAGG  AZ_ad_ARZ63     ATCCTTCG  AZ_ad_ARZ64     ATCCTTGC  AZ_ad_ARZ65     ATCGAACC  AZ_ad_ARZ66     ATCGAAGG  AZ_ad_ARZ67     ATCGATCG  AZ_ad_ARZ68     ATCGATGC  AZ_ad_ARZ69     ATCGCCAA  AZ_ad_ARZ70     ATCGCCTT  AZ_ad_ARZ71     ATCGCGAT  AZ_ad_ARZ72     ATCGCGTA  AZ_ad_ARZ73     ATCGGCAT  AZ_ad_ARZ74     ATCGGCTA  AZ_ad_ARZ75     ATCGTACG  AZ_ad_ARZ76     ATCGTAGC  AZ_ad_ARZ77     ATCGTTCC  AZ_ad_ARZ78     ATCGTTGG  AZ_ad_ARZ79     ATGCAACC  AZ_ad_ARZ80     ATGCAAGG  AZ_ad_ARZ81     ATGCATCG  AZ_ad_ARZ82     ATGCATGC  AZ_ad_ARZ83     ATGCCGAT  AZ_ad_ARZ84     ATGCCGTA  AZ_ad_ARZ85     ATGCGCAT  AZ_ad_ARZ86     ATGCGCTA  AZ_ad_ARZ87     ATGCGGAA  AZ_ad_ARZ88     ATGCGGTT  AZ_ad_ARZ89     ATGCTACG  AZ_ad_ARZ90     ATGCTAGC  AZ_ad_ARZ91     ATGCTTCC  AZ_ad_ARZ92     ATGCTTGG  AZ_ad_ARZ93     ATGGAACG  AZ_ad_ARZ94     ATGGAAGC  AZ_ad_ARZ95     ATGGATCC  AZ_ad_ARZ96     ATGGATGG  AZ_ad_ARZ97     ATGGCCAT  AZ_ad_ARZ98     ATGGCCTA  AZ_ad_ARZ99     ATGGCGAA  AZ_ad_ARZ100    ATGGCGTT  AZ_ad_ARZ101    ATGGTACC  AZ_ad_ARZ102    ATGGTAGG  AZ_ad_ARZ103    ATGGTTCG  AZ_ad_ARZ104    ATGGTTGC  AZ_ad_ARZ105    ATTACCGG  AZ_ad_ARZ106    ATTACGCG  AZ_ad_ARZ107    ATTACGGC  AZ_ad_ARZ108    ATTAGCCG  AZ_ad_ARZ109    ATTAGCGC  AZ_ad_ARZ110    ATTAGGCC  AZ_ad_ARZ111    CAACACCA  AZ_ad_ARZ112    CAACACGT  AZ_ad_ARZ113    CAACAGCT  AZ_ad_ARZ114    CAACAGGA  AZ_ad_ARZ115    CAACCAAC  AZ_ad_ARZ116    CAACCATG  AZ_ad_ARZ117    CAACCTAG  AZ_ad_ARZ118    CAACCTTC  AZ_ad_ARZ119    CAACGAAG  AZ_ad_ARZ120    CAACGATC  AZ_ad_ARZ121    CAACGTAC  AZ_ad_ARZ122    CAACGTTG  AZ_ad_ARZ123    CAACTCCT  AZ_ad_ARZ124    CAACTCGA  AZ_ad_ARZ125    CAACTGCA  AZ_ad_ARZ126    CAACTGGT  AZ_ad_ARZ127    CAAGACCT  AZ_ad_ARZ128    CAAGACGA  AZ_ad_ARZ129    CAAGAGCA  AZ_ad_ARZ130    CAAGAGGT  AZ_ad_ARZ131    CAAGCAAG  AZ_ad_ARZ132    CAAGCATC  AZ_ad_ARZ133    CAAGCTAC  AZ_ad_ARZ134    CAAGCTTG  AZ_ad_ARZ135    CAAGGAAC  AZ_ad_ARZ136    CAAGGATG  AZ_ad_ARZ137    CAAGGTAG  AZ_ad_ARZ138    CAAGGTTC  AZ_ad_ARZ139    CAAGTCCA  AZ_ad_ARZ140    CAAGTCGT  AZ_ad_ARZ141    CAAGTGCT  AZ_ad_ARZ142    CAAGTGGA  AZ_ad_ARZ143    CACAACAC  AZ_ad_ARZ144    CACAACTG  AZ_ad_ARZ145    CACAAGAG  AZ_ad_ARZ146    CACAAGTC  AZ_ad_ARZ147    CACACACA  AZ_ad_ARZ148    CACACAGT  AZ_ad_ARZ149    CACACTCT  AZ_ad_ARZ150    CACACTGA  AZ_ad_ARZ151    CACAGACT  AZ_ad_ARZ152    CACAGAGA  AZ_ad_ARZ153    CACAGTCA  AZ_ad_ARZ154    CACAGTGT  AZ_ad_ARZ155    CACATCAG  AZ_ad_ARZ156    CACATCTC  AZ_ad_ARZ157    CACATGAC  AZ_ad_ARZ158    CACATGTG  AZ_ad_ARZ159    CACTACAG  AZ_ad_ARZ160    CACTACTC  AZ_ad_ARZ161    CACTAGAC  AZ_ad_ARZ162    CACTAGTG  AZ_ad_ARZ163    CACTCACT  AZ_ad_ARZ164    CACTCAGA  AZ_ad_ARZ165    CACTCTCA  AZ_ad_ARZ166    CACTCTGT  AZ_ad_ARZ167    CACTGACA  AZ_ad_ARZ168    CACTGAGT  AZ_ad_ARZ169    CACTGTCT  AZ_ad_ARZ170    CACTGTGA  AZ_ad_ARZ171    CACTTCAC  AZ_ad_ARZ172    CACTTCTG  AZ_ad_ARZ173    CACTTGAG  AZ_ad_ARZ174    CACTTGTC  AZ_ad_ARZ175    CAGAACAG  AZ_ad_ARZ176    CAGAACTC  AZ_ad_ARZ177    CAGAAGAC  AZ_ad_ARZ178    CAGAAGTG  AZ_ad_ARZ179    CAGACACT  AZ_ad_ARZ180    CAGACAGA  AZ_ad_ARZ181    CAGACTCA  AZ_ad_ARZ182    CAGACTGT  AZ_ad_ARZ183    CAGAGACA  AZ_ad_ARZ184    CAGAGAGT  AZ_ad_ARZ185    CAGAGTCT  AZ_ad_ARZ186    CAGAGTGA  AZ_ad_ARZ187    CAGATCAC  AZ_ad_ARZ188    CAGATCTG  AZ_ad_ARZ189    CAGATGAG  AZ_ad_ARZ190    CAGATGTC  AZ_ad_ARZ191    CAGTACAC  AZ_ad_ARZ192    CAGTACTG  AZ_ad_ARZ193    CAGTAGAG  AZ_ad_ARZ194    CAGTAGTC  AZ_ad_ARZ195    CAGTCACA  AZ_ad_ARZ196    CAGTCAGT  AZ_ad_ARZ197    CAGTCTCT  AZ_ad_ARZ198    CAGTCTGA  AZ_ad_ARZ199    CAGTGACT  AZ_ad_ARZ200    CAGTGAGA  AZ_ad_ARZ201    CAGTGTCA  AZ_ad_ARZ202    CAGTGTGT  AZ_ad_ARZ203    CAGTTCAG  AZ_ad_ARZ204    CAGTTCTC  AZ_ad_ARZ205    CAGTTGAC  AZ_ad_ARZ206    CAGTTGTG  AZ_ad_ARZ207    CATCACCT  AZ_ad_ARZ208    CATCACGA  AZ_ad_ARZ209    CATCAGCA  AZ_ad_ARZ210    CATCAGGT  AZ_ad_ARZ211    CATCCAAG  AZ_ad_ARZ212    CATCCATC  AZ_ad_ARZ213    CATCCTAC  AZ_ad_ARZ214    CATCCTTG  AZ_ad_ARZ215    CATCGAAC  AZ_ad_ARZ216    CATCGATG  AZ_ad_ARZ217    CATCGTAG  AZ_ad_ARZ218    CATCGTTC  AZ_ad_ARZ219    CATCTCCA  AZ_ad_ARZ220    CATCTCGT  AZ_ad_ARZ221    CATCTGCT  AZ_ad_ARZ222    CATCTGGA  AZ_ad_ARZ223    CATGACCA  AZ_ad_ARZ224    CATGACGT  AZ_ad_ARZ225    CATGAGCT  AZ_ad_ARZ226    CATGAGGA  AZ_ad_ARZ227    CATGCAAC  AZ_ad_ARZ228    CATGCATG  AZ_ad_ARZ229    CATGCTAG  AZ_ad_ARZ230    CATGCTTC  AZ_ad_ARZ231    CATGGAAG  AZ_ad_ARZ232    CATGGATC  AZ_ad_ARZ233    CATGGTAC  AZ_ad_ARZ234    CATGGTTG  AZ_ad_ARZ235    CATGTCCT  AZ_ad_ARZ236    CATGTCGA  AZ_ad_ARZ237    CATGTGCA  AZ_ad_ARZ238    CATGTGGT  AZ_ad_ARZ239    CCAACCAA  AZ_ad_ARZ240    CCAACCTT  AZ_ad_ARZ241    CCAACGAT  AZ_ad_ARZ242    CCAACGTA  AZ_ad_ARZ243    CCAAGCAT  AZ_ad_ARZ244    CCAAGCTA  AZ_ad_ARZ245    CCAAGGAA  AZ_ad_ARZ246    CCAAGGTT  AZ_ad_ARZ247    CCAATACG  AZ_ad_ARZ248    CCAATAGC  AZ_ad_ARZ249    CCAATTCC  AZ_ad_ARZ250    CCAATTGG  AZ_ad_ARZ251    CCATAACG  AZ_ad_ARZ252    CCATAAGC  AZ_ad_ARZ253    CCATATCC  AZ_ad_ARZ254    CCATATGG  AZ_ad_ARZ255    CCATCCAT  AZ_ad_ARZ256    CCATCCTA  AZ_ad_ARZ257    CCATCGAA  AZ_ad_ARZ258    CCATCGTT  AZ_ad_ARZ259    CCATGCAA  AZ_ad_ARZ260    CCATGCTT  AZ_ad_ARZ261    CCATGGAT  AZ_ad_ARZ262    CCATGGTA  AZ_ad_ARZ263    CCATTACC  AZ_ad_ARZ264    CCATTAGG  AZ_ad_ARZ265    CCGCAATA  AZ_ad_ARZ266    CCGCATAA  AZ_ad_ARZ267    CCGCTATT  AZ_ad_ARZ268    CCGCTTAT  AZ_ad_ARZ269    CCGGAATT  AZ_ad_ARZ270    CCGGATAT  AZ_ad_ARZ271    CCGGATTA  AZ_ad_ARZ272    CCGGTAAT  AZ_ad_ARZ273    CCGGTATA  AZ_ad_ARZ274    CCGGTTAA  AZ_ad_ARZ275    CCTAATCC  AZ_ad_ARZ276    CCTAATGG  AZ_ad_ARZ277    CCTACCAT  AZ_ad_ARZ278    CCTACCTA  AZ_ad_ARZ279    CCTACGAA  AZ_ad_ARZ280    CCTACGTT  AZ_ad_ARZ281    CCTAGCAA  AZ_ad_ARZ282    CCTAGCTT  AZ_ad_ARZ283    CCTAGGAT  AZ_ad_ARZ284    CCTAGGTA  AZ_ad_ARZ285    CCTATACC  AZ_ad_ARZ286    CCTATAGG  AZ_ad_ARZ287    CCTATTCG  AZ_ad_ARZ288    CCTATTGC    UT_Adenylation Domain  =====================  UT_ad_UT1       CCTTAACC  UT_ad_UT2       CCTTAAGG  UT_ad_UT3       CCTTATCG  UT_ad_UT4       CCTTATGC  UT_ad_UT5       CCTTCCAA  UT_ad_UT6       CCTTCCTT  UT_ad_UT7       CCTTCGAT  UT_ad_UT8       CCTTCGTA  UT_ad_UT9       CCTTGCAT  UT_ad_UT10      CCTTGCTA  UT_ad_UT11      CCTTGGAA  UT_ad_UT12      CCTTGGTT  UT_ad_UT13      CGAACCAT  UT_ad_UT14      CGAACCTA  UT_ad_UT15      CGAACGAA  UT_ad_UT16      CGAACGTT  UT_ad_UT17      CGAAGCAA  UT_ad_UT18      CGAAGCTT  UT_ad_UT19      CGAAGGAT  UT_ad_UT20      CGAAGGTA  UT_ad_UT21      CGAATACC  UT_ad_UT22      CGAATAGG  UT_ad_UT23      CGAATTCG  UT_ad_UT24      CGAATTGC  UT_ad_UT25      CGATAACC  UT_ad_UT26      CGATAAGG  UT_ad_UT27      CGATATCG  UT_ad_UT28      CGATATGC  UT_ad_UT29      CGATCCAA  UT_ad_UT30      CGATCCTT  UT_ad_UT31      CGATCGAT  UT_ad_UT32      CGATCGTA  UT_ad_UT33      CGATGCAT  UT_ad_UT34      CGATGCTA  UT_ad_UT35      CGATGGAA  UT_ad_UT36      CGATGGTT  UT_ad_UT37      CGATTACG  UT_ad_UT38      CGATTAGC  UT_ad_UT39      CGCCAATA  UT_ad_UT40      CGCCATAA  UT_ad_UT41      CGCCTATT  UT_ad_UT42      CGCCTTAT  UT_ad_UT43      CGCGAATT  UT_ad_UT44      CGCGATAT  UT_ad_UT45      CGCGATTA  UT_ad_UT46      CGCGTAAT  UT_ad_UT47      CGCGTATA  UT_ad_UT48      CGCGTTAA  UT_ad_UT49      CGGCAATT  UT_ad_UT50      CGGCATAT  UT_ad_UT51      CGGCATTA  UT_ad_UT52      CGGCTAAT  UT_ad_UT53      CGGCTATA  UT_ad_UT54      CGGCTTAA  UT_ad_UT55      CGTAATCG  UT_ad_UT56      CGTAATGC  UT_ad_UT57      CGTACCAA  UT_ad_UT58      CGTACCTT  UT_ad_UT59      CGTACGAT  UT_ad_UT60      CGTACGTA  UT_ad_UT61      CGTAGCAT  UT_ad_UT62      CGTAGCTA  UT_ad_UT63      CGTAGGAA  UT_ad_UT64      CGTAGGTT  UT_ad_UT65      CGTATACG  UT_ad_UT66      CGTATAGC  UT_ad_UT67      CGTATTCC  UT_ad_UT68      CGTATTGG  UT_ad_UT69      CGTTAACG  UT_ad_UT70      CGTTAAGC  UT_ad_UT71      CGTTATCC  UT_ad_UT72      CGTTATGG  UT_ad_UT73      CGTTCCAT  UT_ad_UT74      CGTTCCTA  UT_ad_UT75      CGTTCGAA  UT_ad_UT76      CGTTCGTT  UT_ad_UT77      CGTTGCAA  UT_ad_UT78      CGTTGCTT  UT_ad_UT79      CGTTGGAT  UT_ad_UT80      CGTTGGTA  UT_ad_UT81      CTACACCT  UT_ad_UT82      CTACACGA  UT_ad_UT83      CTACAGCA  UT_ad_UT84      CTACAGGT  UT_ad_UT85      CTACCAAG  UT_ad_UT86      CTACCATC  UT_ad_UT87      CTACCTAC  UT_ad_UT88      CTACCTTG  UT_ad_UT89      CTACGAAC  UT_ad_UT90      CTACGATG  UT_ad_UT91      CTACGTAG  UT_ad_UT92      CTACGTTC  UT_ad_UT93      CTACTCCA  UT_ad_UT94      CTACTCGT  UT_ad_UT95      CTACTGCT  UT_ad_UT96      CTACTGGA  UT_ad_UT97      CTAGACCA  UT_ad_UT98      CTAGACGT  UT_ad_UT99      CTAGAGCT  UT_ad_UT100     CTAGAGGA  UT_ad_UT101     CTAGCAAC  UT_ad_UT102     CTAGCATG  UT_ad_UT103     CTAGCTAG  UT_ad_UT104     CTAGCTTC  UT_ad_UT105     CTAGGAAG  UT_ad_UT106     CTAGGATC  UT_ad_UT107     CTAGGTAC  UT_ad_UT108     CTAGGTTG  UT_ad_UT109     CTAGTCCT  UT_ad_UT110     CTAGTCGA  UT_ad_UT111     CTAGTGCA  UT_ad_UT112     CTAGTGGT  UT_ad_UT113     CTCAACAG  UT_ad_UT114     CTCAACTC  UT_ad_UT115     CTCAAGAC  UT_ad_UT116     CTCAAGTG  UT_ad_UT117     CTCACACT  UT_ad_UT118     CTCACAGA  UT_ad_UT119     CTCACTCA  UT_ad_UT120     CTCACTGT  UT_ad_UT121     CTCAGACA  UT_ad_UT122     CTCAGAGT  UT_ad_UT123     CTCAGTCT  UT_ad_UT124     CTCAGTGA  UT_ad_UT125     CTCATCAC  UT_ad_UT126     CTCATCTG  UT_ad_UT127     CTCATGAG  UT_ad_UT128     CTCATGTC  UT_ad_UT129     CTCTACAC  UT_ad_UT130     CTCTACTG  UT_ad_UT131     CTCTAGAG  UT_ad_UT132     CTCTAGTC  UT_ad_UT133     CTCTCACA  UT_ad_UT134     CTCTCAGT  UT_ad_UT135     CTCTCTCT  UT_ad_UT136     CTCTCTGA  UT_ad_UT137     CTCTGACT  UT_ad_UT138     CTCTGAGA  UT_ad_UT139     CTCTGTCA  UT_ad_UT140     CTCTGTGT  UT_ad_UT141     CTCTTCAG  UT_ad_UT142     CTCTTCTC  UT_ad_UT143     CTCTTGAC  UT_ad_UT144     CTCTTGTG  UT_ad_UT145     CTGAACAC  UT_ad_UT146     CTGAACTG  UT_ad_UT147     CTGAAGAG  UT_ad_UT148     CTGAAGTC  UT_ad_UT149     CTGACACA  UT_ad_UT150     CTGACAGT  UT_ad_UT151     CTGACTCT  UT_ad_UT152     CTGACTGA  UT_ad_UT153     CTGAGACT  UT_ad_UT154     CTGAGAGA  UT_ad_UT155     CTGAGTCA  UT_ad_UT156     CTGAGTGT  UT_ad_UT157     CTGATCAG  UT_ad_UT158     CTGATCTC  UT_ad_UT159     CTGATGAC  UT_ad_UT160     CTGATGTG  UT_ad_UT161     CTGTACAG  UT_ad_UT162     CTGTACTC  UT_ad_UT163     CTGTAGAC  UT_ad_UT164     CTGTAGTG  UT_ad_UT165     CTGTCACT  UT_ad_UT166     CTGTCAGA  UT_ad_UT167     CTGTCTCA  UT_ad_UT168     CTGTCTGT  UT_ad_UT169     CTGTGACA  UT_ad_UT170     CTGTGAGT  UT_ad_UT171     CTGTGTCT  UT_ad_UT172     CTGTGTGA  UT_ad_UT173     CTGTTCAC  UT_ad_UT174     CTGTTCTG  UT_ad_UT175     CTGTTGAG  UT_ad_UT176     CTGTTGTC  UT_ad_UT177     CTTCACCA  UT_ad_UT178     CTTCACGT  UT_ad_UT179     CTTCAGCT  UT_ad_UT180     CTTCAGGA  UT_ad_UT181     CTTCCAAC  UT_ad_UT182     CTTCCATG  UT_ad_UT183     CTTCCTAG  UT_ad_UT184     CTTCCTTC  UT_ad_UT185     CTTCGAAG  UT_ad_UT186     CTTCGATC  UT_ad_UT187     CTTCGTAC  UT_ad_UT188     CTTCGTTG  UT_ad_UT189     CTTCTCCT  UT_ad_UT190     CTTCTCGA  UT_ad_UT191     CTTCTGCA  UT_ad_UT192     CTTCTGGT  UT_ad_UT193     CTTGACCT  UT_ad_UT194     CTTGACGA  UT_ad_UT195     CTTGAGCA  UT_ad_UT196     CTTGAGGT  UT_ad_UT197     CTTGCAAG  UT_ad_UT198     CTTGCATC  UT_ad_UT199     CTTGCTAC  UT_ad_UT200     CTTGCTTG  UT_ad_UT201     CTTGGAAC  UT_ad_UT202     CTTGGATG  UT_ad_UT203     CTTGGTAG  UT_ad_UT204     CTTGGTTC  UT_ad_UT205     CTTGTCCA  UT_ad_UT206     CTTGTCGT  UT_ad_UT207     CTTGTGCT  UT_ad_UT208     CTTGTGGA  UT_ad_UT209     GAACACCT  UT_ad_UT210     GAACACGA  UT_ad_UT211     GAACAGCA  UT_ad_UT212     GAACAGGT  UT_ad_UT213     GAACCAAG  UT_ad_UT214     GAACCATC  UT_ad_UT215     GAACCTAC  UT_ad_UT216     GAACCTTG  UT_ad_UT217     GAACGAAC  UT_ad_UT218     GAACGATG  UT_ad_UT219     GAACGTAG  UT_ad_UT220     GAACGTTC  UT_ad_UT221     GAACTCCA  UT_ad_UT222     GAACTCGT  UT_ad_UT223     GAACTGCT  UT_ad_UT224     GAACTGGA  UT_ad_UT225     GAAGACCA  UT_ad_UT226     GAAGACGT  UT_ad_UT227     GAAGAGCT  UT_ad_UT228     GAAGAGGA  UT_ad_UT229     GAAGCAAC  UT_ad_UT230     GAAGCATG  UT_ad_UT231     GAAGCTAG  UT_ad_UT232     GAAGCTTC  UT_ad_UT233     GAAGGAAG  UT_ad_UT234     GAAGGATC  UT_ad_UT235     GAAGGTAC  UT_ad_UT236     GAAGGTTG  UT_ad_UT237     GAAGTCCT  UT_ad_UT238     GAAGTCGA  UT_ad_UT239     GAAGTGCA  UT_ad_UT240     GAAGTGGT  UT_ad_UT241     GACAACAG  UT_ad_UT242     GACAACTC  UT_ad_UT243     GACAAGAC  UT_ad_UT244     GACAAGTG  UT_ad_UT245     GACACACT  UT_ad_UT246     GACACAGA  UT_ad_UT247     GACACTCA  UT_ad_UT248     GACACTGT  UT_ad_UT249     GACAGACA  UT_ad_UT250     GACAGAGT  UT_ad_UT251     GACAGTCT  UT_ad_UT252     GACAGTGA  UT_ad_UT253     GACATCAC  UT_ad_UT254     GACATCTG  UT_ad_UT255     GACATGAG  UT_ad_UT256     GACATGTC    AB_KSa_Ketosynthase Alpha  =========================  ACGAGTGCGT    AZ_KSa_Ketosynthase Alpha  =========================  ACGCTCGACA    UT_KSa_Ketosynthase Alpha  =========================  AGACGCACTC  ------------------------    Tags used for files GPJCU3H0x and GSISCEJ0x (Total 8 files)    AB-AD AACCGCAT  AB-KS GTCAGAGA  AB-KSa ACGAGTGCGT  AB-16s AACCAACC  AZ-AD AACCGCTA  AZ-KS GTCAGTCA  AZ-KSa AGACGCACTC  AZ-16s AACCAAGG  UT-AD AACCGGAA  UT-KS GTCAGTGT  UT-KSa AACCTACG  UT-16s AACCATCG