title: GSM1704039: GEK37a; Glycine max; RNA-Seq
library_construction_protocol: Total RNA was isolated from the seed material using Trizol (Invitrogen, CA, USA) according to the manufactures’ protocol, and the RNA quality was evaluated by electrophoresis on a 1% agarose gel. Total RNA (> 10 μg) was sent to Fasteris Life Sciences SA (Plan-les-Ouates, Switzerland) for processing. Four paired-end mRNAseq libraries were constructed. Two for each treatment (salt stress and control). Briefly, the polyadenylated transcript sequencing (mRNA-seq) was performed using the following successive steps: Poli-A purification, cDNA synthesis using Poli-T primer shotgun to generate inserts of 500 nt, 3p and 5p pair-end adapters ligations, pre-amplification, colony generation and sequencing. The Illumina output data were sequence tags of 50 bases.
attributes.tag: GEO Accession
attributes.value: GSM1704039