title: GSM1290216: MCF-10A-input rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated and quantified from the cell culture using QIAZoL(Qiagen Inc.). Next, total RNA was enriched for polyadenylated RNA (polyA mRNA).  Poly A+RNA was chemically fragmented into ~100-nucleotide long fragments. A portion of the fragmented RNA was subjected to RNA-seq that served as input (control). The remaining RNA was subjected to immunoprecipitation (IP) with anti-methyl cytosine antibody. The isolated, fragmented, and enriched m5C mRNAs were submitted for sequencing library preparation by using standard Illumina TruSeq Sample Preparation Kits, without poly-A purification step RNA-Seq, 50bp SR by Hiseq 2000
attributes.tag: GEO Accession
attributes.value: GSM1290216