title: GSM1289350: DS20968 SE DNaseI-seq 7 day old seedling Dark 24hr; Arabidopsis thaliana; DNase-Hypersensitivity
library_construction_protocol: DNaseI: Nuclei were extracted and isolated with biotin-capture methods. Nuclei were then treated with 45u of DNase I for 3 minutes at 25°C. Resulting genomic DNA was size fractionated and small (double cut) fragments were sequenced using Illumina technology. RNA-seq 100-200mg of tissue was ground in liquid nitrogen and total RNA was extracted using the Spectrum Plant Total RNA kit (Sigma), RNA was treated with DNase I and Ribo-Zero Plant leaf kit (Epicentre) and libraries were prepared using the TruSeq kit v2 (Illumina). Illumina TruSeq v2
attributes.tag: GEO Accession
attributes.value: GSM1289350