title: Illumina HiSeq 2000 sequencing; Porcine Transcriptome and Methylome Map
design: RRBS DNA methylation single end experiment using bisulfite treated MspI digested 30 - 160 bp fragments on an Illumin HiSeq 2000
attributes.tag: assay type
attributes.value: methylation profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 9
attributes.tag: extraction protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UIUC_SOP_Simultaneous_Purification_of_Genomic_DNA_and_Total_RNA_20170523.pdf
attributes.tag: library preparation location
attributes.value: Carver High-Throughput DNA Sequencing and Genotyping Unit, University of Illinois, Urbana, IL, USA
attributes.tag: library preparation location longitude
attributes.value: 40.103706
attributes.tag: library preparation location latitude
attributes.value: -88.223803
attributes.tag: sequencing location
attributes.value: Carver High-Throughput DNA Sequencing and Genotyping Unit, University of Illinois, Urbana, IL, USA
attributes.tag: sequencing location longitude
attributes.value: 40.103706
attributes.tag: sequencing location latitude
attributes.value: -88.223803
attributes.tag: experiment target
attributes.value: DNA methylation
attributes.tag: library selection
attributes.value: RBBS
attributes.tag: bisulfite conversion protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UIUC_SOP_Complete_Bisulfite_Conversion_and_cleanup_of_DNA_for_Methylation_Analysis_20170523.pdf
attributes.tag: pcr product isolation protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UIUC_SOP_PCR_Product_Isolation_Protocol_20170523.pdf
attributes.tag: bisulfite conversion percent
attributes.value: 99.34
attributes.tag: restriction enzyme
attributes.value: MspI
attributes.tag: min fragment size selection range
attributes.value: 30
attributes.tag: max fragment size selection range
attributes.value: 160