title: SBG of Leptodiaptomus sp.: adult female Quechulac
design: indexed libraries were prepared from double digested DNA with MSPI and NsiI-HF enzymes, following the UMGC standard protocol for SBG library preparation and individual barcoding (Truong et al., 2012). Libraries were combined in a single pool and sequenced on an Illumina NextSeq 550 1  150-bp run. All barcodes were recovered. Each sample produced > 1 M reads, with a mean read quality score (phred) > 30.  Reads were mapped against genomic databases (human, mouse, E. coli, yeast, PhiX virus and chloroplast) to detect DNA contamination.