title: bluehead wrasse sex change
design: The goal is to characterize the whole-transcriptome expressional changes in the brain (forebrain and midbrain) and gonads of bluehead wrasses across the process of female-to-male sex change and to identify differentially expressed genes that may function as putative genetic triggers of sex change and subsequent genetic cascade that re-engineers a female into a male. Total RNA from 29 forebrain/midbrain and 29 gonadal samples (from  3 control females, 5 terminal phase males and 21 intersex fish), were sent to the Otago Genomics and Bioinformatics Facility at the University of Otago under contract to New Zealand Genomics Limited for library construction and RNA sequencing. 58 multiplexed libraries were prepared with the Illumina TruSeq Stranded mRNA Sample Prep Kit and 125-bp paired-end reads were generated using 3 flow cell lanes of an Illumina HiSeq 2500.