title: 16S amplicon sequencing of reagent control
design: The V4 region of the 16S rRNA gene was amplified by using primers 515F and 805R targeting both bacteria and archaea. 25 amplification cycles were employed. Sequencing with 2 x 300 nt was performed using the V3 MiSeq kit on a MiSeq platform (Illumina). Amplification and sequencing was performed by the Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA; Ličge, Belgium).