title: rpoC1 rDNA Amplicons of primarily Prochlorococcus sp and Synechococcus sp.: North Pacific and Atlantic surface water sample
design: DNA gene rpoC1 primers were modified to target only marine Prochlorococcus and Synechococcus lineages, resulting in the primers 5M_newF and SACR1039R. DNA template was added to 20 uL triplicate reactions and amplified using two-step PCR for 35 and 10 cycles.