title: Whole genome sequencing of Homo sapiens : buffy coat DNA: Sample 50
design: "DNA was extracted from buffy coat using the Qiamp Blood DNA Mini Kit. 1ug of DNA was sheared and assembled into whole genome sequencing libraries using the Illumina TruSeq DNA Library Preparation Kit. Samples were sequenced on the Illumina platform. Pass-filter FASTQ files were trimmed using Trimmomatic, aligned with BWA-MEM, duplicate-marked and quality-score recalibrated using GATK 4.1.3.0."
attributes.tag: alignment_software
attributes.value: BWA-MEM 0.7.17