title: NT_404
design: Total RNA of twenty-day-old tobacco seedling samples were extracted using RNeasy Plant extraction Mini Kit (Qiagen, Hilden, Germany) followed by the addition of DNase I (Qiagen, Hilden, Germany) to remove any potential contaminated genomic DNA. For each line, three RNA samples were pooled together to form one RNA sample for subsequent experiments. cDNA library for each pooled sample was constructed for RNA sequencing on an Ion Torrent platform (Life technologies, CA).