title: PacBio sequencing of rice: whole plant 20 days after germination
design: The Poly(A) RNA was isolated from the total RNA using the oligo d(T) magnetic bead binding method and the Poly(A) PuristTM Kit (Thermo Scientific, http://www.lifetechnologies.com/uk/en/home/brands/ambion.html). 0.5 ?g of poly (A) RNA was reversely transcribed into cDNA using the SMART cDNA Library Construction Kit, which uses Long-Distance PCR method to generate full-length double strand cDNA, after first-strand cDNA synthesis (Takara Biotechnology). The SMRT bell library inserted with cDNAs of >=3 kb, collected through 1.0% agarose gel electrophoresis, was constructed using 3.0 ?g size-selected cDNA with the Pacific Biosciences DNA Template Prep Kit 2.0 (Pacific Biosciences).