title: WGS of infant gut metagenome: Estonian subject T016811 at age 390 days
design: DNA extractions from stool were carried out using the QIAamp DNA Stool Mini Kit (QIAGEN, Inc., Valencia, CA, USA). Metagenomic DNA samples were quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50 pg uL-1. Illumina sequencing libraries were prepared from 100-250 pg DNA using the Nextera XT DNA Library Preparation kit (Illumina) according to the manufacturer’s recommended protocol, with reaction volumes scaled accordingly. Insert sizes and concentrations for each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies)