title: P. australis rhizosphere
design: Libraries of nuclear ribosomal ITS2 region were amplified from each DNA extract using ITS1 (5-CTTGGTCATTTAGAGGAAGTAACCTACGGGNGGCWGCAG-3) and ITS4 (5-TCCTCCGCTTATTGATATGCGGACTACHVGGGTWTCTAAT-3) primers fused with Illumina MiSeq adapters and 6-bp barcode sequence (underlined) unique to each sample according to standard protocol