title: small  RNA-seq of Arabidopsis thaliana:  bulked AMIR-1  over-expressing seedlings
design: RNA  were purified with the phenol-chloroform method, 20 g of the samples were  loaded onto poly-acrylamide gel, the small RNA fraction was isolated and  libraries were prepared using the Truseq Small RNA Library Preparation Kit  (Illumina, San Diego, CA, USA) and the modified protocol described earlier  (Czotter et al., 2018).