title: WGS for GBM39
design: Genomic DNA was  extracted from cells using Qiagen kits. Sequencing libraries were prepared  using TruSeq adapters (Illumina) and the KAPA HyperPlus kit, according to  manufacturers instructions (Kapa Biosystems). Briefly, 250 ng of DNA was  used as input and enzyme-fragmented for 12 minutes to obtain mode fragment  lengths of 350 bp. KAPA Pure Beads were used for double-sided size selection  of 250-450 bp. DNA libraries were pooled and paired-end DNA sequencing (150  cycles) was performed.