title: Region_2 contained a 75:25 mix of Maxxa:TX2094 (Nimblegen)
design: A total of four regions on a single 454 PTP plate were used to generate sequence capture data for this pilot experiment.  Region 2 was composed entirely of a mixture of Maxxa (MID1) and TX2094 (MID2) in a 75:25 mix.  Two WGS libraries were constructed by Roche methods from Maxxa and TX2094 DNA. The libraries were independently prepared, subjected to sequence capture and subsequent ligation-mediated PCR to generate sufficient quantities of the captured fragments for sequencing.  The samples were captured using custom Nimblegen Sequence Capture Microarrays. The samples were mixed together after LM-PCR for sequencing.