title: : A. elisabethae microbiome analysis (V4 region)
design: 16S rDNA amplicons were amplified from metagenomic DNA using the primer pair F515 (5′ - GTGCCAGCMGCCGCGGTAA) and R806 (5′ - GGACTACHVGGGTWTCTAAT), which amplify the V4 region of the 16S rRNA gene.Following amplification, the PCR products from different samples were mixed in equal concentrations and purified using Agencourt Ampure beads (Agencourt Bioscience Corporation, MA, USA). Sequencing was performed at MR DNA (Shallowater, TX, USA) utilizing Roche 454 FLX titanium instruments and reagents according to the manufacturer’s guidelines.