title: Whole exome sequencing of Homo sapiens : primary prostate tumor: Sample 189
design: "Regions of tissue were excised from formalin-fixed paraffin-embedded tissues using laser capture microdissection and DNA was extracted using the QIAamp DNA FFPE Tissue Kit. 10-50ng of DNA were sheared and assembled into whole exome sequencing libraries using the SureSelect Human All Exon V7 Low Input Exome kit (Agilent). Samples were sequenced on the Illumina platform. Pass-filter FASTQ files were trimmed using Agilent SureCall Trimmer, aligned with BWA-MEM, duplicate-marked and quality-score recalibrated using GATK 4.1.3.0."
attributes.tag: alignment_software
attributes.value: BWA-MEM 0.7.17