title: WGS of gastric cancer: Sample ESTAD_03_TA_D
design: Genomic library constructed using the KAPA Hyper Prep Kit with PCR (Kapa Biosystems, Wilmington, MA) according to manufacturer protocol.  DNA fragmented with the Covaris E210 and purified between enzymatic reactions. Size selection performed with SPRI-select beads (Beckman Coulter Genomics, Danvers, MA). PCR amplification performed with primers containing an index sequence seven nt in length.