title: RAD-Seq of Anthothoe spp
design: Paired-end libraries were produced from DNA digested with the 6-cutter restriction enzyme PstI following the Rapture protocol of Ali et al. (2016). Libraries barcoded with 10-base pair sequence tags were sequenced on two lanes of an Illumina HiSeq 4000 (150 bp). Raw HiSeq output was de-multiplexed and quality filtered using the process_radtags module (default quality settings; reads truncated to 140 bp) from the STACKS v1.46 pipeline.