title: WGS of Anemopaegma arvense (Voucher Firetti 237)
design: Total DNA was diluted to a final volume of 50 ml at 100 ng/ml, and fragmented by nebulization with 15 psi for 30 seconds. Library was constructed following the GS FLX + Series/XL+ (Roche) kit, which led to fragments that range in size from 1,400 to 1,800 base pairs. Library was quantified with 2100 Bioanalyser (Agilent Technologies, USA). Sequencing produced reads with ca. 700 base pairs.