title: S1
design: Each sample contained bacteria from 10 shared bicycles. After culturing in Brain-Heart Infusion (BHI) broth medium at 37C for 24 h, each sample was centrifuged at 4000 g for 5 min. Afterwards, the pellet was collected, and these subsamples were stored at -80 C until DNA extraction. Total DNA was extracted from each subsample using a soil DNA kit . Each sample was then purified using a DNA purification kit . A Nanodrop spectrophotometer was used to estimate the concentration of the DNA samples, and 1% agarose gel electrophoresis was used to visualize the extracted DNA. PCR amplification of the full-length 16S rDNA was performed with specific barcoded primers, and TransStart FastPfu DNA Polymerase. PCR products were recovered with the AxyPrepDNA gel recovery kit , and the DNA was eluted by Tris-HCl and visualized by 2% agarose electrophoresis. Finally, the PCR products were qualified using QuantiFluor  - ST blue fluorescence quantitative system. Full length purified PCR samples was sequenced by Single Molecule Real-Time (SMRT) technology on a PacBio RS II sequencer. SMRT bell DNA library construction was performed following the manufacturers instructions (PacBio).