title: GTF ChIP-Seq of WT (KOS) and  ICP4 null (n12) HSV-1 Productive Infection in MRC5 cells
design: MRC5 cells were infected with WT  (KOS) or ICP4-null HSV-1 (n12) at an MOI of 10 PFU/cell for 2.5 hours and  ChIP-Seq for ICP4, Pol II, TBP, Sp1, Med1, and Med23 was performed. Illumina  libraries were constructed for the IP and input material. Illumina sequencing  was carried out at Tufts University Core Facility using HiSeq 2500 to  generate 50 bp single-end reads.