title: culture of Leisingera aquaemixtae R2C4: PacBio genome sequence
design: DNA from a pure culture of R2C4 was used to generate a ~10 kb insert size library which was sequenced using 105 SMRT cells (1X180 minutes movie) on PacBio RSII using C3-P5 chemistry (DNALink, Korea). All DNA was size selected (>7 kb) using the Blue Pippin system (Sage Sciences) and samples were sheared with g-TUBEs (Covaris).