title: Whole Genome of COLO829BL lymphoblastoid line  from a metastasis from a cutaneous melanoma male patient: Sample COLO829BL_GSC
design: Library preparation and sequencing. 1.1µg of DNA from each line was used to generate whole genome libraries using the Illumina TruSeq DNA PCR-free LT Sample Prep Kit (Set A; cat#FC-121-3001) following the manufacturer’s protocol. Following sonication on the Covaris E210, 100ng of each sample was electrophoretically separated on a 1% TAE gel to verify fragmentation. The remaining fragmented 1µg of each sample was used to generate libraries. Final libraries were evaluated on the Agilent Bioanalyzer and quantitated by Qubit. Libraries were clustered onto HiSeq V3 rapid flowcells using the Illumina TruSeq Rapid PE Cluster Kit (2500; cat#PE-402-4001) and sequenced by synthesis on the HiSeq2500 (rapid mode) for paired 112bp read lengths using Illumina TruSeq Rapid SBS Kits (2500; cat#FC-402-4002).
attributes.tag: alignment_software
attributes.value: BWA - 0.7.8