title: Rhizosphere microbiome
design: Root was shaken in 50ml flask filled with sterile distilled water for 1 minute. 2 ml were then sampled from flask and centrifuged at 14000g for 5 minutes. 0.2g DNA were extracted from pellet using PureSoil DNA extraction kit. Purified DNA template was amplified using universal multiplex primers F515 and R806 targeting the 16S rRNA V4 hyper-variable region.