title: 4C-seq for GBM39 rep1
design: Five million cells were cross-linked  with 2% formaldehyde for 10 min at room temperature and quenched by 125 mM  glycine for 5 min. Nuclei were isolated and digested with Csp6I (Thermo  Scientific) overnight. Enzyme was inactivated by heating at 65C for 20 min  and the digested chromatin was subjected for ligation by T4 ligase (Life  Technologies) for 16 h. DNA was then purified with before the second  digestion with DpnII (NEB) overnight. After enzyme inactivation, a second  round of ligation was performed, and DNA was purified. 4.8 g of DNA in total  was used for PCR amplification.