title: RNA-Seq of Solenopsis invicta: midden pile replicate 2
design: Total RNA purified from dead workers collected from midden piles was used for mRNA purification with the Illumina TruSeq Stranded mRNA Library Preparation Kit (Catalog # RS-122-2101).  The low sample protocol was followed according to the manufacturers instructions.  The RNA fragmentation step was omitted to maximize library insert length.  Library size was determined empirically by microfluidic analysis on an Agilent 2100 Bioanalyzer and quantified using the Quant-iT dsDNA kit with broad range standards (ThermoFisher Scientific, Q-33130).  Samples were pooled together at equimolar quantities and sequenced twice using the Illumina MiSeq (2x300) cycle kit with version 3 chemistry.  Using the Illumina indices, the data were demultiplexed and the runs combined to assign the data to individual samples.  All other procedures were followed according to the manufacturers instructions.