title: RNA-Seq of human: A549 cell line in normoxia
design: For RNA-seq [34], the poly(A)-containing mRNA was purified using Oligo(dT) Beads (Illumina), and this was followed by fragmentation. The converted doublestranded cDNA product was subjected to blunt-ending, dA addition to the 3 end and adapter ligation. The adapter-ligated fragments were size selected (200  20 bp) using 2 % TAECertified Low-Range Ultra Agarose (BioRad). After purification, 15 rounds of PCR amplification were performed to enrich the adapter-ligated cDNA libraries. The LHC-BS and RNA-seq libraries were performed on an Agilent Technologies 2100 Bioanalyzer using the Agilent DNA 1000 chip kit. They were subsequently quantified on a StepOne plus qPCR, and library products were sequenced using the Illumina Hiseq X ten.