title: Steelhead Transcriptome Sequencing -  H4B
design: Notes: tissue collection: whole body fry were ground under liquid nitrogen, and RNA was extracted using Trizol, followed by RNA clean-up using a Qiagen Rneasy kit. Poly-(A) mRNA was purified using the Ambion PolyA-Purist kit and prepared for sequencing using standard Illumina multiplexing protocol. Multiplex for sample H4B: TCGATT