title: Adult Sea Lamprey Brain
design: RNA samples were extracted using TRIzol method according to the manufacturer’s instruction (Invitrogen). RNA were then poly-A selected, fragmented, reverse transcribed to cDNA, and sequenced using an Illumina GA2 machine by MSU Research Support and Technology Facility using the Illumina mRNA-Seq 8 sample prep kit and yielding 20 million 75 base reads per sample for a total of 1.5 gb of mRNA-Seq sequence.