title: Illumina Sequencing for Thermoanaerobacterium saccharolyticum M2886
design: . Illumina TruSeq libraries were prepared as described in the manufacturer’s methods (Part# 15005180 RevA) following the low throughput protocol. In short, 3 ug of DNA was sheared to a size between 200 bp and 1,000 bp by nebulization using nitrogen gas for 1 min at 30 psi. Sheared DNA was purified on a Qiagen Qiaquick Spin column (Qiagen). The sheared material was assessed for quantity with a Qubit broad range double stranded DNA assay (Life Technologies) and quality by visualization on an Agilent Bioanalyzer DNA 7500 chip (Agilent). One microgram of sheared DNA was used for library preparation following the manufacturer’s protocol. Libraries were validated by Qubit (Life Technologies) and Agilent Bioanalyzer for appearance and size determination. Samples were normalized using Illumina’s Library dilution calculator to a 10nM stock and diluted further for sequencing. Clustering was completed on an Illumina CBot, and paired end sequencing was completed on an Illumina HiSeq instrument (101 bp for each end and 7 bp for the index) using TruSeq sequencing-by-synthesis chemistry