title: siRNA from the sweet potato leaf phytobiome found in three cultivars in Barbados
design: Total RNA was extracted from two infected leaf samples of the sweet potato cultivar CBS32 using TRIZOL and subjected to Next-Generation DNA Sequencing (NGS) using Ilumina technology which was carried out by Fasteris Inc. Genome processing consisted of separation of RNA by 3.5% agarose gel electrophoresis and isolation of RNA bands <30 nucleotides (nts), followed by ligation with single stranded 3’ and 5’ adapters. These ligated fragments were then reverse transcribed and amplified by PCR to generate a library of short inserts.