title: 16S rRNA sequencing of Aedes aegypti adult female midguts
design: All DNA was sequenced at MR DNA (Shallowater, TX, USA) where an Illumina MiSeq platform (San Diego, CA, USA) was used for sequencing the bacterial 16S rRNA gene. Briefly, the V4-V5 hyper variable region was targeted via PCR, which was performed using the HotStartTaq Plus Master Mix Kit (Qiagen, Hilden, Germany) under the following conditions: 94C for 3mins, 28 cycles of 94C for 30sec, 53C for 40sec and 72C for 1min, after which a final elongation step at 72C for 5min was performed. After amplification, PCR products were checked for relative intensity on a 2% agarose gel. Samples were then purified using calibrated Agencourt AMPure XP beads (Brea, CA, USA), and run on the MiSeq platform using the manufacturers protocols.