title: Microbiomes of free-living ciliates
design: 16S DNA libraries were prepared according to Illumina workflow http://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf with primers targeting the V3 and V4 regions of the SSU ribosomal RNA (rRNA) gene such as forward F515 and reverse R907 (Zhou et al., 2011). The libraries were sequenced in MiSeq (Illumina) using 2  300 bp paired-end MiSeq v3 reagent kit.