title: WGS of Stylosanthes macrocephala: adult cv. Campo Grande
design: Sequencing library was generated using NEBNextUltra II DNA Library Prep Kit for Illumina (New England Biolabs, England) and following manufactures recommendations. The genomic DNA was randomly fragmented to a size of 350bp by Bioruptor, then DNA fragments were narrowly size selected with sample purification beads. The selected fragments were then end polished, A-tailed, and ligated with the full-length adapter. After these treatments, these fragments were filtered with beads again. At last, the library was analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR. Libraries were sequenced by Illumina high-throughput NovaSeq 6000 sequencer with paired-end (2150bp) sequencing strategy