title: sRNAs of Araucaria engustifolia
design: Total RNA was extracted from Brazilian pine fresh leaves. RNA sequencing was performed using the Illumina HiSeq2000 platform, one sRNA library was constructed and sequenced, comprising 28,376,092 single-end reads with a length of 50 bases. The sRNA library building follows a series of standard steps, briefly described as follows: gel purification of the RNA corresponding to fragments ranging from 20-30 nt, ligation of the 3p and 5p adapters separately and followed by gel purification each, cDNA synthesis and cDNA gel purification, and, finally, PCR amplification to generate a cDNA colony template library for deep sequencing.