title: RNAseq for GBM39 rep2
design: One microgram RNA extracted by RNeasy mini kit (QIAGEN) was  prepared for sequencing with TruSeq RNA Library Prep Kit v2 (Illumina)  according to the manufacturers instruction. Briefly, after poly-A selection  and fragmentation of the total RNA, first and second strand cDNA was  synthesized and ligated with sequencing adapter. Products were then amplified  for paired-end sequencing.