title: lncRNA-Seq of Mus musculus: 25 week old, cardiac tissue, mitochondria
design: RNA, isolated from human atrial appendages and mouse whole heart (as described above), was sequenced through the West Virginia University Genomics Core Facility. Briefly, RNA was selected for long non-coding RNA analysis using a Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA). Samples were run on the HiSeq (Illumina) in 51bp paired end reads achieving ~30 million clustered reads per sample. Generated Fastq files were processed through HISAT2 under standard parameters, with the exception of quality scoring control specified under --phred33. The reference genome used in generating BAM files included fasta formatted DNA from Ensemble release 95 for human (GRCh38) and mouse (GRCm38).