title: ONT seq of P. fluviatilis : adult male blood
design: DNA was extracted using a phenol/chloroform protocol and gDNA quality and purity was assessed using spectrophotometry, fluorometry and capillary electrophoresis. Additional purification steps were performed using AMPure XP beads (Beckman Coulter). Library preparation and sequencing was performed using Oxford Nanopore Ligation Sequencing Kit SQK-LSK108 or SQK-LSK109 according to manufacturer's instructions. At each step, DNA was quantified using the Qubit dsDNA HS Assay Kit (Life Technologies). For each library, DNA was purified then sheared at 20 kb using the megaruptor system (diagenode). A DNA damage repair step was then performed on 5 microgram of sheared gDNA and an END-repair and dA tail was performed on double stranded DNA fragments. Adapters were ligated and the libraries were loaded at a concentration of 0.005 to 0.1 pmol and sequenced for 48 h on 11 GridION R9.4 or R9.4.1 flowcells.