title: PARE of Spirodela polyrhiza ABA 3 hours 2
design: A degradome (PARE) library was created with the GMUCT2.0 protocol. mRNA was polyA selected, and a 5' RNA adapter bound to uncapped mRNA and used for reverse transcription, followed by <15 rounds of library barcoded PCR. Size selection and purification steps throughout. Agilent bioanalyzer and Qbit QC to check RNA integrity.