title: RNA-Seq of Trichosanthes kirilowii for transcriptome assembly
design: RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina by following manufacturerŐs recommendations.  The sequencing libraries were multiplexed and clustered onto a flowcell. After clustering, the flowcell was loaded on the Illumina HiSeq 2500 instrument according to manufacturerŐs instruction