title: Illumina HiSeq 2000 paired end sequencing; Porcine Transcriptome and Methylome Map
design: RNA-Seq transcriptomic paired end experiment using mRNA enrichment on an Illumina HiSeq 2500
attributes.tag: assay type
attributes.value: RNA-seq of coding RNA
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 9
attributes.tag: extraction protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UIUC_SOP_Simultaneous_Purification_of_Genomic_DNA_and_Total_RNA_20170523.pdf
attributes.tag: library preparation location
attributes.value: Carver High-Throughput DNA Sequencing and Genotyping Unit, University of Illinois, Urbana, IL, USA
attributes.tag: library preparation location longitude
attributes.value: 40.103706
attributes.tag: library preparation location latitude
attributes.value: -88.223803
attributes.tag: sequencing location
attributes.value: Carver High-Throughput DNA Sequencing and Genotyping Unit, University of Illinois, Urbana, IL, USA
attributes.tag: sequencing location longitude
attributes.value: 40.103706
attributes.tag: sequencing location latitude
attributes.value: -88.223803
attributes.tag: experiment target
attributes.value: polyA RNA
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: library generation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: read strand
attributes.value: mate 2 sense