title: Small RNA-Seq of rice: Shoot_-N_0d
design: 15 ug of total RNA was used for 15% urea-gel fractionation of 18 to 30-nt small RNA pool. 3' and 5' adapter were sequentially ligated to fractionated small RNAs, and ligated products were reverse-transcribed with SuperScript II (Invitrogen). Synthesized cDNAs were amplified by PCR using Phusion Polymerase (NEB) and amplified PCR products were gel-fractionated using 6% native PAGE-gel electrophoresis.