title: RNA-Seq of Fusarium sp. HFEW-16-IV-019: pH 7: BioRep 1
design: RNA-seq of Fusarium sp.  HFEW-16-IV-019 grown 14 days in Potato Dextrose Broth (PDB) at pH 7; total RNA was isolated from 200 mg homogenized tissue according to the manufacturer's protocol and using a Norgen RNA Purification Kit (Norgen Biotek Corporation). Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. NRS1222002) following manufacturer's recommendations and index codes were added to identify each sample. The libraries were sequenced on the NextSeq 500 platform (Illumina) using the 2x150 bp paired-end sequencing protocol.