title: RNA-Seq of sus scrofa: DLY female control 1 (WT)
design: Integrity and quantity of RNA was evaluated by Agilent 2100, following the manufacturers instructions. After DNase I treatment, mRNA was isolated by magnetic beads with Oligo (dT). Mixed with the fragmentation buffer, mRNA was fragmented into short fragments. cDNA was synthesized using the mRNA fragments as templates, resolved with EB buffer for end reparation and ligated with adapters.