title: WGS of Anemopaegma arvense (Voucher Firetti 241)
design: Total DNA was diluted to a final volume of 50 µl at 100 ng/ml and sonicated using Covaris S-series with the following parameters: 175W of incident power, 10% of duty factor, 200 cycles and 50 s. Fragmented sample was used to prepare pair-ended libraries using TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, USA), following the manufacturerÕs protocol. Library was quantified via qPCR using KAPA Library Quantification Kit for Illumina Sequencing Platform (Kapa Biosystems, Wilmington, USA) on an ABI7300 RT-PCR. Sample was diluted in ddH2O to a final concentration of 2 nM and 5 µl of diluted libraries were pooled together (8 samples total). The final pool was sequenced at the Centro de Facilidades de Apoio ˆ Pesquisa (CEFAP Ð USP, S‹o Paulo, Brazil) using MiSeq Reagent Kit v.3 kit. Fragment size: ~550 bp. Read size: 300 bp.