title: 16S Amplicon Sequencing of Cabernet Sauvignon grape rhizosphere from V U FincaArriba vintage 2015
design: Rhizosphere soil was scraped from roots with sterile tweezers. DNA was extracted from 0.4 g rhizosphere soil using FastDNA Spin Kit for Soil (MP Biomedicals). The V3-V4 region was amplified using primers 341F and 806R with Illumina adapters. Illumina indexed adapters were used in a second PCR to add an index