title: Bioremediation of heavy metals
design: Genome sequencing steps includes isolation and purification of genomic DNA, fragmentation, ligation to sequencing adapters and purification. Following the amplification and denaturation steps, libraries can be pooled and sequenced. We used 50 ng of DNA from to prepare the libraries using Nextera DNA Sample Preparation Kit (Illumina). Library insert size was determined by Experion Automated Electrophoresis Station (Bio-Rad). The insert size of the libraries ranged from 300 to 850 bp (average 500bp). Pooled library (12pM) was loaded to a 600 Cycles v3 Reagent cartridge (Illumina) and the sequencing was performed on Miseq (illumina)