title: Halophile diversity of Marakkanam saltpan
design: For Oxford nanopore sequencing, 1 g purified PCR template was used. Sequencing library was prepared according to manufacturer protocol by end-repair/ dA-tailing reaction, barcode and adapter ligation, and final purification using AMPure XP bead binding (Oxford Nanopore Technologies). The sequencing was performed by priming and loading the sample on a SpotOn flow cell in MinION sequencer (Oxford Nanopore Technologies).