title: WGBS of Nipponbare: 1-y-callus WT  replicate 1
design: High-quality genomic DNA of the 1-  and 3-y callus cultures of the three genotypes were isolated by a modified  CTAB method (Kidwell and Osborn, 1992), and used to perform whole genome  resequencing. The library construction, cluster generation and sequencing  were performed at the Beijing Genomics Institute (BGI, Shenzhen, China) and  carried out with standard procedures. The sequencing platform was Illumina  Hi-Seq 2000 sequencing platform. The sequencing strategy was 100 bp pair-end  (PE) with 500bp library size.