title: Exome-Seq of human: tumor: Sample P092T
design: "The Library prep process starts with genomic DNA isolated from FFPE Tissue being sheared using a Covaris ultrasonicating device to reach the desired fragment size. The ends of the DNA fragments are repaired to produce blunt-ended, 5'-phosphorylated fragments. A-tailing to the DNA fragment is performed next, dAMP is added to the 3'-ends of blunt-ended dsDNA library fragments. Sequencing adapters are ligated to the fragments during which dsDNA adapters with 3'-dTMP overhangs are ligated to 3'-dA-tailed library fragments. Library amplification is then performed, amplification employs high-fidelity, low-bias PCR to amplify library fragments carrying appropriate adapter sequences on both ends. The samples are QC'd and now ready for capture."