title: UCE target enrichment of Pitohui_dichrous_KU_12200
design: We sheared 500 ng of DNA of each sample in 50 _l volume using a Covaris S220 sonicator. We performed _ reactions of end-repair, A-tailing and adapter-ligation using Kapa Biosystems Library Prep kits and custom single-indexed adapters. Libraries were combined in pools of 8 equimolar samples for enrichment. We performed target enrichment using the Mycroarray MYbaits kit for Tetrapods UCE 5K version 1, which targets 5,060 UCE loci. Fragments recovered were amplified in a 17-cycle PCR amplification step, quantified using an Illumina Eco qPCR System, and sequenced in a high output paired-end run of 100 cycles on an Illumina HiSeq 2500 System.