title: RNA-Seq of Megaptera novaeangliae blow
design: RNA was extracted using RNeasy Plus Universal mini kit (Qiagen). Due to low RNA concentration, all 19 samples were pooled and concentrated using a NucleoSpin RNA Clean-up XS kit (Macherey-Nagel). A single library was produced for RNA sequencing using the Low Input SMARTer Stranded Total RNA Sample Prep Kit with Mammalian rRNA depletion (Clontech), with 1ng of the pooled whale blow RNA as input. Paired-end (100bp) sequencing of the RNA library was performed on the HiSeq 2500 platform (Illumina) at the Australian Genome Research Facility.