title: ATACseq for COLO320DM rep2
design: Briefly, 100-500K cell nuclei were  extracted by NPB buffer (5% BSA, 0.2% IGEPAL-CA630, 1 mM DTT, EDTA-free  protease, in PBS) at 4C for 10 min. Tagmentation was done in TB buffer (33  mM Tris-acetate pH 7.8, 66 mM K-acetate, 11 mM Mg-acetate, 16% DMF) with Tn5  transposase (Illumina, San Diego, CA), at 37C for 30 min. DNA samples were  then extracted and DNA libraries were generated by PCR.