title: "Variability in Microbial Community Structure during Development of a Nutrient-Cycling Model Ecosystem, sample 8MR31"
design: "Sediment and water samples were collected from Blackford Pond in Edinburgh and used to make multiple replicate microcosms of various sizes. 1 ml sediment-water slurry from microcosms from different sized microcosms at different time intervals used in DNA extraction using the UltraClean Soil DNA Isolation Kit (MoBio, Carlsbad, CA, USA)."