title: scRNA of human adult male bone_marrow relapse tumor: Sample 452198_RelPostAllo.scRNA
design: Cells were processed using the 10x Genomics Chromium Controller and the Chromium Single Cell Library & Gel Bead Kit following the standard manufacturer's protocols (https://tinyurl.com/y96l7lns). Gel beads were prepared according to standard manufacturer's protocols. Oil partitions of single-cell + oligo coated gel beads (GEMs) were captured and reverse transcription was performed, resulting in cDNA tagged with a cell barcode and unique molecular index (UMI). Next, GEMs were broken and cDNA was amplified and quantified using an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). To prepare the final libraries, amplified cDNA was enzymatically fragmented, end-repaired, and polyA tagged. Fragments were then size selected using SPRIselect magnetic beads (Beckman Coulter). Next, Illumina sequencing adapters were ligated to the size-selected fragments and cleaned up using SPRIselect magnetic beads (Beckman Coulter). Finally, sample indices were selected and amplified, followed by a double sided size selection using SPRIselect magnetic beads (Beckman Coulter). Final library quality was assessed using an Agilent Bioanalyzer High Sensitivity chip.
attributes.tag: alignment_software
attributes.value: Cellranger