title: small RNA sequencing of 21UR-3922m in active insertion site 2
design: ~2 ug of total RNA were pretreated with 1.5 ul RNA-pyrophosphohydrolase (Rpph, NEB) in a total volume of 20 ul for 1h at 37C.  The RNA was phenol-chloroform extracted, ethanol/AcNA precipitated overnight, resuspended in water and used as an input for TruSeq small RNA library preparation kit from Illumina according to manufacturer's instructions.