title: HiSeq of Pseudomonas aeruginosa
design: DNA was quantified in triplicate with the Quantit dsDNA high sensitivity assay in an Eppendorf AF2200 plate reader. Genomic DNA libraries were prepared using Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: 2 ng of DNA instead of 1ng were used as input, and PCR elongation time was increased to 1 minute from 30 seconds. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system. Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina on a Roche light cycler 96 qPCR machine. Libraries were sequenced on the Illumina HiSeq 2500 using a 250 bp paired end protocol