title: Deep Sequencing of Off Target Site 3 in untreated blastocyst
design: Sites of partial homology to the PNA binding site were identified. Blastocysts then underwent whole genome amplification. Amplicons were then generated and nucleotide changes from the reference genome were analyzed. The primers used for off target site 3 were (forward first): (5-NNN NNN AAGCTCAAACCTACCAGACCA-3 and 5-NNN NNN AGCTGGAAGCTTCTTCAGTCA-3). Each N is a random base.