title: RNA-Seq of Homo sapiens: MCF-7 cell line
design: Extract protocol - Total MCF-7 RNA was ordered and received from Ambion/Life Technologies AM7846 as starting material. Library construction protocol - Illumina TruSeq RNA Library Prep Kit after ribosomal depletion using Epicentre RiboZero kit and ~600bp shearing. We used 2x300 bp paired end strategy for sequencing on the MiSeq for the 2x300 bp libraries using v3 reagents.