title: Vibrio cholerae crtS_VC23; exponential culture sample
design: This sample was intended for Marker Frequency Analysis (MFA). In this strain the crtS was deleted from its entopic locus and reinstated at an ectopic locus near gene VC0023 on the large chromosome, chr1. Replication of the crtS locus triggers replication of the small chromosome, chr2. Cells were grown in LB (+ glucose) at 30°C under agitation. Genomic DNA was prepared from exponentially growing cells harvested at OD450 ~0.15. The remaining culture was kept under agitation at 30°C to determine the generation time corresponding to each sample. Sequencing libraries were prepared for sequencing by automat, targeting an insert size of 200 base pairs bp. Libraries were sequenced using an Ion Proton sequencer PI chip. MFA was performed essentially as described in Skovgaard et al. (2011) PMID: 21555365; doi: 10.1101/gr.117416.110.