title: Bulked Segregant Analysis of soybean
design: Young leaves were collected at V7 stage and quickly frozen in liquid nitrogen. DNA from 49 M2 individuals with the shorter trichome phenotype and 60 M2 individuals with wild type phenotype were bulks into the mutant and wild type two pools in an equal ratio. A total of 2 g genomic DNA for each pool was prepared for constructing a paired-end sequencing library. Paired-end sequencing libraries with an insert size of approximately 350 bp were sequenced on an Illumina HiSeq X ten sequencer .