title: Soil bacteria, Remnant, Nov2012, Casa
design: The V4 hyper variable region of the 16s rRNA gene was amplified using the F563-R802 primers fused with 454 adapters within the same primer. Each sample was amplified by PCR reactions and pooled in equimolar concentrations before sequencing on a Genome Sequencer FLX (454-Roche Applied Sciences) using Titanium Chemistry according to the manufacturers instructions.