title: 16S amplicon sequencing of nasal wash solution
design: The V4 region of the 16S rRNA gene was amplified by using primers 515F and 805R targeting both bacteria and archaea. 30 amplification cycles were employedand the amplicon copy number was determined by qPCR and isomolecular mixtures of the libraries were prepared for the sequencing runs. Sequencing with 2 x 300 nt was performed using the V3 MiSeq kit on a MiSeq platform (Illumina). Amplification and sequencing was performed by the Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA; Ličge, Belgium).