title: WGS of homo sapiens: adult male Whole Blood: Sample GTEX-RUSQ-0001-SM-5JK34
design: Genomic DNA input into shearing was reduced from 3 ug to 100 ng in 50 uL of solution. Illumina paired-end adapters were replaced with palindromic forked adapters with unique 8-base index sequences embedded within the adapter. Size selection was performed using Sage's Pippin Prep, with a target insert size of 340 bp or 370 bp +/- 10% (both sizes were selected). Libraries were quantified with qPCR using the KAPA Library Quantification Kit, normalized to 2 nM, and then denatured using 0.1 N NaOH with Perkin-Elmer's MultiProbe liquid handling platform. Cluster amplification of the templates was performed according to the manufacturer's protocol using HiSeq 2000 v2 or v3 cluster chemistry. Flow cells were sequenced on HiSeq 2000 following the manufacturer's protocol.
attributes.tag: alignment_software
attributes.value: bwamem 0.7.13-r1126