title: RNA-Seq of rattus norvegicus: primary cell  culture from hippocampus after treatment with miRNA
design: Cells were lysed in RLT puffer and total RNA was extracted using the RNeasy Mini Kit (Qiagen). 30 ng total RNA was converted to cDNA using the Ovation RNA-Seq System V2 (Nugen Technologies) according to manufacturers protocol. This cDNA was used to construct sequencing libraries using Ovation Rapid DR Multiplex System (Nugen Technologies) according to manufacturers protocol.