title: RNA-Seq of Gymnoprosopa
design: Extractions were performed with the RNeasy kit (Qiagen, Valencia, CA). Library preparation used the Illumina Truseq Library Prep Kit. RNA was bound to Agencourt AMPure XP Beads (Beckman Coulter, Inc., Brea, CA, USA) on a magnetic plate. A reverse transcription reaction is performed, followed by a PCR enrichment, yielding a size-selected non-directional cDNA library to be sequenced as paired-end reads on an Illumina system (Illumina, San Diego, CA, USA)