title: 16S sequencing of decomposing leaf litter  samples: longterm Global Change Experiment
design: A longterm global change field experiment  site was used to collect leaf litter samples. The samples were collected from  ambient or reduced water plots and from ambient or added nitrogen plots. Leaf  litter DNA was isolated and bacterial communities were targeted with the EMP  primers. The PCR products were sequenced on the Illumina miseq platform using  the v3 PE300.