title: RNAseq of STTM2118b.5 old leaves rep 5
design: RNAseq libraries were prepared using the Truseq mRNA HT kit (Illumina). In brief, total RNA from three different conditions (young: 3 weeks old leaves, old: 6 weeks old leaves, and infected: 3 day post inoculation of detached leaves with P. infestans) with six biological replicates each. Libraries were prepared using 1 g of total RNA per sample. PolyA bead selection and strand-specific RNA-seq libraries were made and indexed according to manufacturer instructions. Finalized libraries were sequenced as a pool on one lane of a NextSeq 500/550 High Output Kit v2 (75+75 cycles).