title: HiSeq of P. fluviatilis : adult male blood
design: Illumina short reads libraries were built using the Truseq nano kit (Illumina, ref. FC-121-4001) following the manufacturers instructions. First, 200 ng of gDNA was briefly sonicated using a Bioruptor sonication device (Diagenode, Liege, Belgium), end-repaired and size-selected on beads to retain fragments of size around 550 bp, and these fragments were A-tailed and ligated to Illuminas adapter. Ligated DNA was then subjected to eight PCR cycles. Libraries were checked with a Fragment Analyzer (AATI) and quantified by qPCR using the Kapa Library quantification kit. Libraries were sequenced on one lane of a HiSeq2500 using the paired end 2x250 nt v2 rapid mode according to the manufacturers instruction. Image analysis was performed with the HiSeq Control Software and base calling with the RTA software provided by Illumina.