title: RNA-Seq of Cryptocotyle lingua: cercariae from the host 1
design: Cercariae and rediae of Cryptocotyle lingua (Creplin, 1825) (Digenea: Heterophyidae) were obtained from a first intermediate host, a common periwinkle Littorina littorea (Linnaeus, 1758). Three pairs of cercarial and redial samples were collected from three naturally infected mollusks captured near BBS Kartesh (Chupa inlet, Kandalaksha bay of the White Sea). RNA was extracted using TRIzol, cDNA libraries were generated following the Illumina TruSeq Stranded mRNA LT Sample Prep Kit protocol, and then performed 2x100bp paired-end RNA sequencing on an Illumina HiSeq 2000. Three paired biological replicates were sequences: rediae and cercariae from the same infected periwinkle in one pair.