title: Parus major BoneMarrow RNA-Seq data
design: Tissues (bone marrow, homogenized half of the brain, breast filet, higher intestine, kidney, liver, lung and testis) were collected, snap-frozen and stored for RNA isolation at -80 C. RNA isolation was performed on 25 mg thawed tissue segments using the miRNeasy mini kit from Qiagen, following the protocol of the manufacturer. For library preparation we used the standard Illumina TruSeq RNAseq protocol using a different barcoded adapters (Illumina) for each of the samples. Sequencing: Samples have been sequenced as pool, using 5 lanes on one flowcell, (same run on the machine) in Illumina HiSeq 2000. This resulted in 100 bp paired end unstranded RNAseq data. The number of reads per tissue ranged from 86 to 229 million  with a total  number of 1.29 billion paired end reads. This sample contains Arabidopsis contamination (about 15% of the reads).