title: Region_1 of a 454 plate with Maxxa
design: A total of four regions on a single 454 PTP plate were used to generate sequence capture data for this pilot experiment.  Region 1 was composed entirely of TX2094.  TX2094 fragments were captured using a custom Nimblegen Sequence Array containing DNA probes targeting 534 genes.  The library was independently made using the traditional WGS kit from Roche.  After capture, the library fragments were amplified (LM-PCR).  We titered the number of LM-PCR cycles needed by 1st amplifying a test sample (30 ul) and sampling the amount of amplified product every 5 cycles on a Lonza Electorphoresis system to avoid over-amplification.