title: ChIP-seq of humans: child and adult peripheral blood mononuclear cells: Sample seq6557_2
design: "Raw sequence reads were obtained in fastq format from the Illumina MISeq instrument in the UVA Biomolecular Core facility.  Reads were mapped to the hg19 reference genome using Bowtie 1.0.0.  Peak calling was performed using MACS 1.4.2 with gender-specific input control datasets.  Datasets for children at 18-weeks or 52 weeks were analyzed separately, as was the maternal data.  The peak calls for each dataset were used as input to DESeq2 v1.6.3 implemented in R v3.0.2.  Significantly affected peaks were computationally linked to genes using GREAT.  Downstream analysis of the associated genes employed a variety of tools as described in the publication, including GOrilla, MSigDB, GSEA, Pathway Express, ConsensusPathDB, and DAVID."
attributes.tag: alignment_software
attributes.value: Bowtie 1.0.0