title: ssRNA-seq Phaeodatylum tricornutum high CO2 2
design: A total amount of 3 g RNA per sample was used as initial material for the RNA sample preparations. Ribosomal RNA was removed using Epicentre Ribo-Zero Gold Kits(Human/Mouse/Rat)Epicentre, USA.Subsequently, the sequencing libraries were generated following manufacturer recommendations with varied index label by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA) .