title: Vibrio cholerae Delta_crtS_D247; exponential culture sample
design: This sample is a pre-evolution control for a Short-Term Evolution Experiment and was intended for Marker Frequency Analysis (MFA) and mutation detection. In this strain the locus crtS was deleted from the large chromosome, chr1. Replication of the crtS locus triggers replication of the small chromosome, chr2. Cells were grown exponentially in Mueller Hinton medium (MH) at 37°C under agitation. Genomic DNA was prepared from exponentially growing cells harvested at OD450 ~0.15. Sequencing libraries were prepared for sequencing by automat, targeting an insert size of 200 base pairs bp. Libraries were sequenced using an Ion Proton sequencer PI chip. MFA was performed essentially as described in Skovgaard et al. (2011) PMID: 21555365; doi: 10.1101/gr.117416.110.