title: sesame anther growth and development transcriptome
design: total RNA was from abnormal anther with male sterile of ms86-1.   1 mRNA binding  2 Fragment the mRNA  3 Synthesize the First Strand cDNA  4 Synthesize the Second Strand cDNA  5 End Repair   6 Add 'A' Bases to the 3' End of the DNA Fragments  7 Ligate Adapters to DNA Fragments  8 Enrich the Adapter-Modified DNA Fragments by PCR