title: RNA-seq of Akkermansia muciniphila
design: RNA-seq (2G raw data  per sample) was performed by Novogen using HiSeq platforms with paired-end  150 bp. Illumina reads have been trimmed for low quality and adapters with  fastp (v0.20.0) using default settings. rRNA sequences have been removed with  bbduk(v38.35) (https://sourceforge.net/projects/bbmap/) using the following  parameters k=31 and ref=riboKmers.fa.gz.