title: RNA-Seq of  monocytes of human blood
design: Clean reads were obtained after remove of low quality reads and removing reads with  5' linker sequence, reads without 3' linker sequence, reads without the insert tag, reads with poly (A), and reads shorter than 16 nt. The clean reads were compared to the miRbase21 and Rfam database to identify known miRNA and other non-coding RNAs. The raw count for known miRNAs were summarized and then normalized using reads per million mapped reads (RPM). Total clean reads were also mapped to the human reference genome using the bwa aligner. The mapped reads were further annotated to the repeats and exon regions based on the genome annotation.