title: chicken manure digastate
design: DNA was extracted using the cetyltrimethyl ammonium bromide (CTAB) / sodium dodecyl sulfate (SDS) method and purified by 1% agarose gel. DNA was diluted to 10 ng/L and used in PCR for amplification of bacterial  16S rRNA genes ,the  Primers are 338F (5'-ACT CCT ACG GGA GGCAGC AG -3') and 806R (5'-GGA CTA CHVGGG TWT CTA AT -3').