title: RNA-seq of Macaca mulatta: adult male colon
design: Total RNA was isolated from duodenal, jejunal, ileal, and colonic biopsies using the Qiagen mRNeasy kit (Qiagen, Valencia, CA). Ribosomal RNA (rRNA) was depleted using the Epicentre Ribo-Zero rRNA Removal kit (Illumina, Madison, WI). Libraries were constructed using the Bioo Scientific NEXTflex Rapid Directional RNA-seq kit (Bioo Scientific, Austin, TX).