title: Zoysia matrella F1 mapping population derived from a cross between cultivars 'Diamond' and 'Cavalier'
design: The RADseq libraries were constructed using the protocol as mentioned. Approximately 1μg of genomic DNA was digested with restriction enzymes NsiI (New  England Biolabs, Ipswich, MA) and MseI (New England Biolabs, Ipswich, MA). The digested genomic DNA was ligated with P1 and P2 adapters containing unique identifying sequences (molecular identifier, MID, see Table S1). The ligation reaction was deactivated at 65°C for 20 min, and then purified using Axyprep Mag PCR clean-up beads (Axygen, Union City, CA) following the manufacturer’s instruction. Purified adaptor-ligated DNA was amplified by PCR (12 cycles) with Phusion High Fidelity DNA polymerase (New England Biolabs, Ipswich, MA).  Then, size selection of 300-500 bp DNA fragments were performed using Axyprep Mag PCR clean-up beads (Axygen, Union City, CA) following the manufacturer’s instruction. Recovered  DNA was quantified using a Qubit fluorometer with the dsDNA HS kit (Life Technologies, Carlsbad, CA). The RADseq libraries of the parental plants and the 95 progeny were pooled and sequenced on an Illumina HiSeq 2000 (Illumina, San Diego, CA).