title: 16S rRNA amplicon sequences: low-DO-AOA sludge- AOA_16S rRNA_D0
design: 16S rRNA gene fragments in the DNA extract were amplified using barcoded primers for sequencing library preparation. The barcoded primer pair 341F/805R (341F: 5-CCT ACG GGN GGC WGC AG-3; 805R: 5-GAC TAC GVG GGT ATC TAA TCC-3) was used to amplify the 16S rRNA gene fragments in both the domain bacteria and archaea (Herlemann et al. 2011). 16S rRNA amplicons were sequenced using high-throughput Illumina MiSeq sequencing platform (Illumina, U.S.).