title: GVHD.27.Lean.1
design: Mouse microbiome analysis DNA was isolated using the Qiagen DNeasy PowerSoil kit (Qiagen). Primers 319F and 806R were used to amplify the V3-V4 domain of the 16S rRNA. Each sample was barcoded with a unique forward and reverse barcode combination using Illumina P5 adapter sequence.