title: NextSeq 500 sequencing
design: MuSCs were isolated from muscles of WT animals as described by Guenther et. al (Cell Stem Cell. 2013 Nov 7;13(5):590-601). MuSCs were transduced with Lentivirus encoding V5-tagged Duxbl for 12 hours and subsequently grown in proliferation medium (DMEM,  20%FCS; Pen/Strep; 2.5 ng/ml bFGF). Emerging SC Duxbl clones were then extracted and further processed.