title: 16S sequencing of  decomposing leaf litter samples: longterm Global Change Experiment
design: A longterm global  change field experiment site was used to collect leaf litter samples. The  samples were collected from ambient or reduced water plots and from ambient  or added nitrogen plots. Leaf litter DNA was isolated and bacterial  communities were targeted with the EMP primers. The PCR products were  sequenced on the Illumina miseq platform using the v3 PE300.