title: 16S metagenome from the abdomen of Xyleborus bispinatus reared with Platanus mexicana
design: Ribosomal amplicons of the variable V3 region of the 16S gene was amplified from five nanograms per microliter of total DNA extracted from the abdomen of Xyleborus bispinatus reared on an artificial diet prepared with Mexican Sycamore (Platanus mexicana) sawdust. Illumina libraries were constructed by adding Nextera XT adapters (Illumina Inc., U.S.A.). Amplicon PCR and adapter-ligation PCR were performed using Kapa polymerase (Kappa HiFi Hotstart Ready Mix, cat. No. KK2602, Kappa Biosystems) following the manufacturers recommendations and purified immediately after finishing each PCR using 0.8X Agencourt Ampure XP cleaning beads (BeckmanCoulter, cat. No. A63881). Library concentration was quantified using a DNA HS kit (Invitrogen, cat. No. Q32854) in a Qubit 2.0 fluorometer (Invitrogen, cat. No. Q32866). Adapter ligation and library size were evaluated using a Bioanalyzer (Agilent, cat. No. G2953CA) DNA HS (Agilent, cat. No. 5067-4626) chip. Libraries were diluted and pooled to an equimolar concentration to be denatured and loaded in a MiSeq (Illumina Inc., U.S.A.) using a MiSeq Reagent kit V3 (Illumina Inc. cat. No. 15067874).