title: mRNA-seq of Col-0 A. thaliana stem
design: Total RNA was isolated using Tri-Reagent (Sigma) and used as input to the Smart-seq2 method of library preparation (Picelli et al. 2014 - Nat. Protoc.). Smart-seq2 libraries were prepared by amplifying full length transcripts using target-switching oligo (TSO) reverse transcription and tagmented using the Illumina Nextera DNAFlex kit. Fragments were then amplified using barcoded primers for tags, quality-controlled, and pooled for single-end, 75nt sequencing on an Illumina NextSeq550 instrument using the high-output kit. Libraries were deconvoluted by barcodes.