title: RNA-Seq of Sus scrofa:  infant descending colon exfoliome
design: Using 100 ng of total RNA in NuGen's Ovation 3' DGE Sytem following manufacturer's instructions.  The cDNA from this step was fragmented using the Covaris then 800 - 1000 ng of this fragmented cDNA was used in the Encore Rapid Library System kit from NuGen.  The final library was prepped following the manufacturer's instructions.