title: RNA-Seq_Mops_condylurus_spleen:PMA_Ion
design: Single cell spleen suspensions from one male Mops condylurus were seeded into 96-well plates and stimulated with Phorbol myristate acetate (PMA) and Ionomicin (Ion). After six hours in culture, the RNA was extracted with use of TRIzol reagent and stored at -80.  RNA from each stimulation method was barcoded separately and paired end sequencing performed. Reads were processed with QCumber2 pipeline to remove bases with a quality score of less than 30 and remove adapters. Paired and unpaired trimmed reads as fastq.gz files are provided here in SRA. The forward, unpaired reads were utilized for de novo assembly, which was carried out with Trinity (v2.4.0).