title: Single rat hippocampal neuron cell, amplificaiton by multiple displacement amplification, MDA‐3
design: ￼Hippocampal neurons were prepared from individual E18 rat embryos and cultured in neurobasal medium as described previously. Nuclei of individual hippocampal neurons were collected using a glass micropipette and transferred directly to 200‐μL PCR tubes. The single neuron nucleus was then subjected to whole genome amplification using multiple displacement amplification (MDA). Sequencing libraries were constructed following the Illumina standard protocol and sequenced by an Illumina HiSeq 2000.