title: WGS for COLO320DM
design: Genomic DNA was extracted from cells  using Qiagen kits. Sequencing libraries were prepared using TruSeq adapters  (Illumina) and the KAPA HyperPlus kit, according to manufacturers  instructions (Kapa Biosystems). Briefly, 250 ng of DNA was used as input and  enzyme-fragmented for 12 minutes to obtain mode fragment lengths of 350 bp.  KAPA Pure Beads were used for double-sided size selection of 250-450 bp. DNA  libraries were pooled and paired-end DNA sequencing (150 cycles) was  performed.