title: Amplicon of china harbor water
design: Illumina MiSeq amplicon sequencing was performed on all samples. The 16S rRNA gene sequencing primers 314F (5-CCTACGGGAGGCAGCAG-3) and 807R (5GGACTACHAGGGTATCTAAT-3) that cover the V3 and V4 rRNA regions were used to amplify the bacterial fraction of extracted DNA according to previous protocols (Prosdocimi et al., 2013). Samples were prepared using the Illumina workflow for 16S rRNA gene analysis (16S Metagenomic Sequencing Library Preparation, Illumina Inc.). Samples were normalized, pooled, and run on a paired-end MiSeq platform using V3 sequencing technology.