title: Region_4 contained Maxxa and TX2094 (50:50)
design: A total of four regions on a single 454 PTP plate were used to generate sequence capture data for this pilot experiment.  Region 4 was composed of a mixture of Maxxa (MID1) and TX2094 (MID2) in 50:50 molar amounts prior to a common emPCR pool to prepare a single 'library' for sequencing.  The fragments of both libraries were independently captured using a custom Nimblegen Sequence Array containing DNA probes targeting 534 genes.  Each library was independently made using the traditional WGS kit from Roche.  After capture, the library fragments were amplified (LM-PCR).  We titered the number of LM-PCR cycles needed for each library by 1st amplifying a test sample (30 ul) and sampling the amount of amplified product every 5 cycles on a Lonza Electorphoresis system to avoid over-amplification.