title: ITS Amplicon Sequencing of Cabernet Sauvignon grape rhizosphere from V U FincaArriba vintage 2017
design: Rhizosphere soil was scraped from roots with sterile tweezers. DNA was extracted from 0.4 g rhizosphere soil using FastDNA Spin Kit for Soil (MP Biomedicals). The rRNA internal transcribed spacer region was amplified using primers ITS1 and ITS2 with Illumina adapters. Illumina indexed adapters were used in a second PCR to add an index