title: Arabidopsis wild-type and oxt6 mutant poly(A) tags
design: These tags were prepared as described in Wu et al. (Wu X, Liu M, Downie B, Liang C, Ji G, Li QQ, Hunt AG. Genome-wide landscape of polyadenylation in Arabidopsis provides evidence for extensive alternative polyadenylation. Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12533-8.). Briefly, cDNA was prepared using an anchored oligo-dT primer flanked by a sequence compatible with Ilumina sequencing.  Double-stranded cDNA was digested with one of two restriction enzymes that recognize a four-base sequence, Illumina-compatible adapters were ligated to the digested cDNA, and the resulting tags amplified and submitted for sequencing.      This sample contains two sets of bar-coded tags .  For wild-type tags, the 5'-end (AHQL02_1) tags possessed either a CATG or ACGT sequence at their ends, followed immediately by the cDNA sequence.  The 3'-end (AHQL01_2) tags possess a code consisting of NNNCC, followed by an oligo-dT tract of up to 18 nts.  For oxt6 tags, the 5'-end (AHQL02_1) tags possessed either a CTCATG or CTACGT sequence at their ends, followed immediately by the cDNA sequence.  The 3'-end (AHQL02_2) tags possess a code consisting of NNNAC, followed by an oligo-dT tract of up to 18 nts.