title: Genomic DNA sequencing of Ixodes scapularis clone ISE6 Cell Line
design: (1) Library Sizing by Pippin Prep.(2) NextGen Paired End Barcoded Genomic Library Construction.(3) NextGen Library quantification and normalization by qPCR.  Genomic DNA was isolated from the cell line using a Qiagen genomic DNA isolation kit.  Bioanalyzer analysis confirmed high molecular DNA was recovered.  Sample was sequenced on Next Generation Sequencing platforms.  Reads were demultiplexed which removed barcodes and sequencing adapters.