title: RNA-Seq of mus musculus: whole lymph node with PBS
design: 250 ng of whole node RNA was used as input in a TruSeq Stranded mRNA library prep kit (Illumina) to generate cDNA libraries. Both libraries were quantified using a Qubit Fluorimeter (Thermo Fisher Scientific) and multiplexed samples were run on a HiSeq 2500 instrument (Illumina). Using 101 bp paired end sequencing we allocated ~40 million reads per library index.