title: Metatranscriptomics of GOM dead zone: site E2
design: After quantification and quality assessment with the Invitrogen qubit and Agilent Bioanalyzer, RNA is treated with Turbo DNA-free DNase (Life Technologies, Inc.). After removal of DNA, the rRNA was removed utilizing EpicentreŐs Ribo-Zero Bacteria Kit. Following removal of rRNA, the RNA was then checked again on the Agilent bioanalyzer to determine whether rRNA removal was sufficient.   Following rRNA removal, mostly mRNA remains, and a sequencing library was generated with the mRNA using EpicentreŐs ScriptSeq v2 RNA-Seq Library Kit. Libraries were then sequenced on 2x100bp lanes of the Illumina HiSeq2000.