title: "soil sample G2678, PCR primers: fwd-SSU817F, rev-S1196": Sample G2678_2
design: "40 pooled samples (50 mm diam to 5 cm depth) from 2500-m2 plot; 2 g DNA extracted using MoBio Soil Maxi kit; PCR targeting ITS region of fungi using MID-tagged primers (fwd: TTAGCATGGAATAATRRAATAGGA, rev: MID-TCTGGACCTGGTGAGTTT); SequalPrep Normalisation; adapter ligation; emPCR"