title: Amplicon sequencing of Bouteloua grass root mycobiome
design: Genomic DNA amplified using fITS7 and ITS4 primers. Primary PCR conditions: 98 C for 30s, 30 cycles of 94 C for 10s, 52 C for 10s, 72 C for 1 min, and a final extension at 72 C for 5 minutes. Secondary PCR performed on the purified amplicons (10 l as template) using fITS7 and ITS4 primers with sample-specific 12bp molecular identifier (MID) tags for five cycles identical to the primary PCR. The resultant sequence data were contiged, quality-filtered, trimmed to equal length, de-replicated, checked for chimeric sequences, and clustered into operational taxonomic units (OTUs) using the mothur pipeline