title: GSM3752769: S1P; Homo sapiens; RNA-Seq
library_construction_protocol: Cytoplasmic lysates of HeLa cells treated with siRNAs and transfected with or without poly(I:C) were fractionated on a sucrose gradient into 10 fractions. Fractions 1-5 were pooled and fractions 6-to 10 were pooled to form subpolysomal and polysomal pools. RNA was extracted by first precipitating the RNA overnight in ethanol and finally exctacted using Trizol LS. The libraries were enriched for polyA tailed RNA libraries were prepared using the Illumina Truseq protocol (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM3752769