title: RAD-Seq in O. tshawytscha from Alsek - Goat Creek, AK
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-07NF0001 Alsek - Goat Creek - Northern BC - AK border RL-0053 AATGAG  OtsCORE-07NF0002 Alsek - Goat Creek - Northern BC - AK border RL-0053 AGTAGG  OtsCORE-07NF0004 Alsek - Goat Creek - Northern BC - AK border RL-0053 CCATTT  OtsCORE-07NF0005 Alsek - Goat Creek - Northern BC - AK border RL-0053 CTACAG  OtsCORE-07NF0006 Alsek - Goat Creek - Northern BC - AK border RL-0053 GCAGAT  OtsCORE-07NF0007 Alsek - Goat Creek - Northern BC - AK border RL-0053 GTAAGT  OtsCORE-07NF0009 Alsek - Goat Creek - Northern BC - AK border RL-0053 TCAAAG  OtsCORE-07NF0010 Alsek - Goat Creek - Northern BC - AK border RL-0053 TTATGA  OtsCORE-07NF0011 Alsek - Goat Creek - Northern BC - AK border RL-0053 ACAAGA  OtsCORE-07NF0012 Alsek - Goat Creek - Northern BC - AK border RL-0053 AGTTAA  OtsCORE-07NF0013 Alsek - Goat Creek - Northern BC - AK border RL-0053 CCCGGT  OtsCORE-07NF0014 Alsek - Goat Creek - Northern BC - AK border RL-0053 CTCGCC  OtsCORE-07NF0015 Alsek - Goat Creek - Northern BC - AK border RL-0053 GCATGG  OtsCORE-07NF0016 Alsek - Goat Creek - Northern BC - AK border RL-0053 GTATCC  OtsCORE-07NF0017 Alsek - Goat Creek - Northern BC - AK border RL-0053 TCCTGC  OtsCORE-07NF0018 Alsek - Goat Creek - Northern BC - AK border RL-0053 TTCCGT  OtsCORE-07NF0019 Alsek - Goat Creek - Northern BC - AK border RL-0053 ACAGCG  OtsCORE-07NF0020 Alsek - Goat Creek - Northern BC - AK border RL-0053 ATAGTA  OtsCORE-07NF0021 Alsek - Goat Creek - Northern BC - AK border RL-0053 CCCTAA  OtsCORE-07NF0022 Alsek - Goat Creek - Northern BC - AK border RL-0053 CTGCGA  OtsCORE-07NF0023 Alsek - Goat Creek - Northern BC - AK border RL-0053 GCCGTA  OtsCORE-07NF0024 Alsek - Goat Creek - Northern BC - AK border RL-0053 GTCATC  OtsCORE-07NF0025 Alsek - Goat Creek - Northern BC - AK border RL-0053 TCGATT  OtsCORE-07NF0026 Alsek - Goat Creek - Northern BC - AK border RL-0053 TTCTAG  OtsCORE-07NF0027 Alsek - Goat Creek - Northern BC - AK border RL-0053 ACATAC  OtsCORE-07NF0028 Alsek - Goat Creek - Northern BC - AK border RL-0053 ATCAAA  OtsCORE-07NF0030 Alsek - Goat Creek - Northern BC - AK border RL-0053 CCGAGG  OtsCORE-07NF0031 Alsek - Goat Creek - Northern BC - AK border RL-0053 CTGGTT  OtsCORE-07NF0032 Alsek - Goat Creek - Northern BC - AK border RL-0053 GCGACC  OtsCORE-07NF0033 Alsek - Goat Creek - Northern BC - AK border RL-0053 GTGCCT  OtsCORE-07NF0034 Alsek - Goat Creek - Northern BC - AK border RL-0053 TCGCCA  OtsCORE-07NF0035 Alsek - Goat Creek - Northern BC - AK border RL-0053 TTGAGC  OtsCORE-07NF0036 Alsek - Goat Creek - Northern BC - AK border RL-0053 ACCATG  OtsCORE-07NF0037 Alsek - Goat Creek - Northern BC - AK border RL-0053 ATGCAC  OtsCORE-07NF0038 Alsek - Goat Creek - Northern BC - AK border RL-0053 CCGCAT  OtsCORE-07NF0039 Alsek - Goat Creek - Northern BC - AK border RL-0053 CTTATG  OtsCORE-07NF0040 Alsek - Goat Creek - Northern BC - AK border RL-0053 GCGCTG  OtsCORE-07NF0041 Alsek - Goat Creek - Northern BC - AK border RL-0053 GTGTAA  OtsCORE-07NF0042 Alsek - Goat Creek - Northern BC - AK border RL-0053 TCGGAC  OtsCORE-07NF0043 Alsek - Goat Creek - Northern BC - AK border RL-0053 TTTAAT  OtsCORE-07NF0045 Alsek - Goat Creek - Northern BC - AK border RL-0053 ACCCCC  OtsCORE-07NF0046 Alsek - Goat Creek - Northern BC - AK border RL-0053 ATGTTG  OtsCORE-07NF0047 Alsek - Goat Creek - Northern BC - AK border RL-0053 CCTAAC  OtsCORE-07NF0048 Alsek - Goat Creek - Northern BC - AK border RL-0053 CTTTGC  OtsCORE-07NF0049 Alsek - Goat Creek - Northern BC - AK border RL-0053 GCTCAA  OtsCORE-07NF0050 Alsek - Goat Creek - Northern BC - AK border RL-0053 GTTGGA  OtsCORE-07NF0051 Alsek - Goat Creek - Northern BC - AK border RL-0053 TCTCGG