title: RAD-Seq in O. tshawytscha from Wenatchee River, WA & John Day, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  Bonn-CH-042409-1.09 JohnDay RL-0090 AATGAG  BonnCH042710-4.02 JohnDay RL-0090 AGTTAA  BonnCH042711-2.09 JohnDay RL-0090 CTCGCC  BonnCH042910-4.09 JohnDay RL-0090 ACAAGA  BonnCH042911-1.06 JohnDay RL-0090 CCCGGT  BonnCH050310-4.02 JohnDay RL-0090 TTATGA  BonnCH050310-4.08 JohnDay RL-0090 TCAAAG  BonnCH050410-1.07 JohnDay RL-0090 GTAAGT  BonnCH050610-1.01 JohnDay RL-0090 GCAGAT  BonnCH051310-5.01 JohnDay RL-0090 CTACAG  Bonn-CH-051409-1.04 JohnDay RL-0090 AGTAGG  Bonn-CH-052209-2.03 Wenatchee RL-0090 ACTCTT  Bonn-CH-052609-5.07 Wenatchee RL-0090 ATTCCG  BonnCH052610-1.07 JohnDay RL-0090 CCATTT  Bonn-CH-052809-2.10 Wenatchee RL-0090 CGAGGC  Bonn-CH-052809-4.09 Wenatchee RL-0090 GAAATG  Bonn-CH-052909-1.05 Wenatchee RL-0090 GGACTT  Bonn-CH-052909-3.03 Wenatchee RL-0090 TAAGCT  Bonn-CH-060109-3.05 Wenatchee RL-0090 AACGTT  Bonn-CH-060109-3.09 Wenatchee RL-0090 TCTTCT  Bonn-CH-060209-1.10 Wenatchee RL-0090 CAAAAA  Bonn-CH-060209-2.01 Wenatchee RL-0090 TAATTC  Bonn-CH-060209-2.06 Wenatchee RL-0090 GGCAAG  Bonn-CH-060209-2.07 Wenatchee RL-0090 ACTGGC  Bonn-CH-060209-2.10 Wenatchee RL-0090 CGCAGA  Bonn-CH-060209-4.04 Wenatchee RL-0090 GAACCA  Bonn-CH-060309-3.05 Wenatchee RL-0090 AACTGA  Bonn-CH-060509-2.01 Wenatchee RL-0090 CAATCG  Bonn-CH-060509-3.02 Wenatchee RL-0090 AGCCAT  Bonn-CH-060509-4.01 Wenatchee RL-0090 CGCGTG  Bonn-CH-060809-2.03 Wenatchee RL-0090 GGGCGC  Bonn-CH-060809-3.06 Wenatchee RL-0090 GACGAC  Bonn-CH-060909-3.07 Wenatchee RL-0090 TACACA  Bonn-CH-060909-3.08 Wenatchee RL-0090 TGACAA  Bonn-CH-060909-6.01 Wenatchee RL-0090 AAGACG  Bonn-CH-061009-1.01 Wenatchee RL-0090 AGCGCA  Bonn-CH-061009-1.09 Wenatchee RL-0090 CACCTC  Bonn-CH-061009-2.01 Wenatchee RL-0090 GACTCT  Bonn-CH-061009-2.02 Wenatchee RL-0090 CGGTCC  Bonn-CH-061109-4.01 Wenatchee RL-0090 GGGGCG  Bonn-CH-061509-3.02 Wenatchee RL-0090 TACGGG  Bonn-CH-061509-3.04 Wenatchee RL-0090 AAGCTA  Bonn-CH-061509-5.01 Wenatchee RL-0090 TGCCCG  Bonn-CH-061609-2.07 Wenatchee RL-0090 AGGGTC  Bonn-CH-061709-2.04 Wenatchee RL-0090 CAGGCA  Bonn-CH-061709-4.02 Wenatchee RL-0090 CGTCTA  Bonn-CH-061809-2.01 Wenatchee RL-0090 GAGAGA  Bonn-CH-061809-3.10 Wenatchee RL-0090 GGTACA  Bonn-CH-062209-2.08 Wenatchee RL-0090 TAGTAT  Bonn-CH-062409-1.08 Wenatchee RL-0090 TGCTTA  Bonn-CH-062409-2.02 Wenatchee RL-0090 AATATC  Bonn-CH-062509-1.03 Wenatchee RL-0090 CATACT  Bonn-CH-062509-2.02 Wenatchee RL-0090 AGGTGT  Bonn-CH-062509-3.09 Wenatchee RL-0090 CGTGAT  Bonn-CH-062909-1.05 Wenatchee RL-0090 GATCGT  Bonn-CH-063009-1.05 Wenatchee RL-0090 TATCAC  Bonn-CH-071808-3.02 Wenatchee RL-0090 AAACGG  Bonn-CH-072409-1.05 Wenatchee RL-0090 TGGGGA