title: GSM3753415: MNK3-Control_IKZF3; Mus musculus; OTHER
library_construction_protocol: Cut and Run extracts were prepared as in published protocol (Skene, Henikoff, Henikoff - 2018, Nature Protocols). using 2 ug of antibody, 2 M cells per C&R. Briefly, MNK3 cells were permeabalized with 0.02% of DMSO-dissolved digitonin, bound to conA-beads, incubated with antibody, protein-A Mnase (a gift from Heinkoff), and finally chromatin was digested with ice-cold EDTA for 25 minutes. Antibodies were as follows: H3K27ac (abcam ab4729), IKZF3 (Biolegend 371002) and rabbit-anti-mouse IgG (invitrogen 31188). Antibody or pa-MN incubation, as well as washes, were done at room temperature for 5 minutes without rotation. Nextera DNA Library Preparation Kit, Illuminia
attributes.tag: GEO Accession
attributes.value: GSM3753415