title: GSM4189762: 67d; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Each adult fly midguts were dissected in ice-cold 1XPBS/1%BSA solution.sample were washed for 2 times on ice with 1xPBS/1%BSA. 500 μL of 0.5%Trypsin-EDTA solution was add.Samples were  incubated by gentle rotating at room temperature for 20 min.The cells in suspension were carefully removed and filtered through a 40 µm nylon mesh into a fresh tube. The cells were spined down at 100 x g for 5 min at 4 °C, and finally resuspended in PBS with 0.04% BSA at a concentration of approximately 500 cells/µl. The libraries of single cells were prepared using  Gel Bead Kit v2 Chip Kit (10x genomics). RNA sequencing was performed on an Illumina XTEN. Single-cell RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM4189762