title: RAD-Seq in O. tshawytscha from McKenzie Hatchery, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10OG0003 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AAACGG  OtsCORE-10OG0006 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 ATTCCG  OtsCORE-10OG0008 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CGAGGC  OtsCORE-10OG0010 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GAAATG  OtsCORE-10OG0012 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GGACTT  OtsCORE-10OG0013 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TAAGCT  OtsCORE-10OG0022 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 ACTGGC  OtsCORE-10OG0026 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CAAAAA  OtsCORE-10OG0031 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CGCAGA  OtsCORE-10OG0032 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GAACCA  OtsCORE-10OG0034 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GGCAAG  OtsCORE-10OG0036 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TAATTC  OtsCORE-10OG0037 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TGAACC  OtsCORE-10OG0038 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AACTGA  OtsCORE-10OG0042 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AGCCAT  OtsCORE-10OG0043 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CAATCG  OtsCORE-10OG0045 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CGCGTG  OtsCORE-10OG0048 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GACGAC  OtsCORE-10OG0055 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GGGCGC  OtsCORE-10OG0058 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TACACA  OtsCORE-10OG0060 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TGACAA  OtsCORE-10OG0062 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AAGACG  OtsCORE-10OG0063 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AGCGCA  OtsCORE-10OG0065 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CACCTC  OtsCORE-10OG0067 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CGGTCC  OtsCORE-10OG0069 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GACTCT  OtsCORE-10OG0072 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GGGGCG  OtsCORE-10OG0073 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TACGGG  OtsCORE-10OG0075 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TGCCCG  OtsCORE-10OG0076 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AAGCTA  OtsCORE-10OG0077 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AGGGTC  OtsCORE-10OG0079 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CAGGCA  OtsCORE-10OG0082 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CGTCTA  OtsCORE-10OG0084 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GAGAGA  OtsCORE-10OG0090 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GGTACA  OtsCORE-10OG0091 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TAGTAT  OtsCORE-10OG0092 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TGCTTA  OtsCORE-10OG0093 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AATATC  OtsCORE-10OG0099 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AGGTGT  OtsCORE-10OG0101 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CATACT  OtsCORE-10OG0103 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 CGTGAT  OtsCORE-10OG0106 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GATCGT  OtsCORE-10OG0110 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 GGTTTG  OtsCORE-10OG0118 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 TATCAC  OtsCORE-10OG0120 McKenzie Hatchery. 43863 Greer Drive. Leaburg OR 97489 RL-0048 AATGAG  OtsZnlo11_1133 McKenzie Hatchery  RL-0048 ACTCTT  OtsZnlo11_1759 McKenzie Hatchery  RL-0048 AACGTT  OtsZnlo11_4043 McKenzie Hatchery  RL-0048 TCTTCT