title: RAD-Seq in O. tshawytscha from  Nason Creek, Chiwawa River & White River
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsWENcr08-061208-001 Chiwawa River RL0009 ACAAGA  OtsWENcr08-062508-001 Chiwawa River RL0009 AGTTAA  OtsWENcr08-062808-001 Chiwawa River RL0009 CCCGGT  OtsWENcr08-063008-001 Chiwawa River RL0009 CTCGCC  OtsWENcr08-070608-001 Chiwawa River RL0009 GCATGG  OtsWENcr08-070808-001 Chiwawa River RL0009 GTATCC  OtsWENcr08-071008-001 Chiwawa River RL0009 TCCTGC  OtsWENcr08-071208-001 Chiwawa River RL0009 TTCCGT  OtsWENcr08-071808-001 Chiwawa River RL0009 ACAGCG  OtsWENcr08-073108-001 Chiwawa River RL0009 ATAGTA  OtsWENnr07-060807-001 Nason Creek RL0009 GAGAGA  OtsWENnr07-061507-001 Nason Creek RL0009 GGTACA  OtsWENnr07-062007-001 Nason Creek RL0009 TAGTAT  OtsWENnr07-062307-001 Nason Creek RL0009 TGCTTA  OtsWENnr07-062607-001 Nason Creek RL0009 AATATC  OtsWENnr07-062907-001 Nason Creek RL0009 AGGTGT  OtsWENnr07-070407-001 Nason Creek RL0009 CATACT  OtsWENnr07-070407-002 Nason Creek RL0009 CGTGAT  OtsWENnr07-070507-001 Nason Creek RL0009 GATCGT  OtsWENnr07-071307-001 Nason Creek RL0009 GGTTTG  OtsWENnr09-062509-001 Nason Creek RL0009 CCCTAA  OtsWENnr09-062909-001 Nason Creek RL0009 CTGCGA  OtsWENnr09-070309-001 Nason Creek RL0009 GCCGTA  OtsWENnr09-070509-001 Nason Creek RL0009 GTCATC  OtsWENwr07-062107-001 White River RL0009 TATCAC  OtsWENwr07-062307-001 White River RL0009 TGGGGA  OtsWENwr07-062507-001 White River RL0009 AATGAG  OtsWENwr07-070507-001 White River RL0009 AGTAGG  OtsWENwr07-070507-002 White River RL0009 CCATTT  OtsWENwr07-071307-001 White River RL0009 CTACAG  OtsWENwr07-071407-001 White River RL0009 GCAGAT  OtsWENwr07-071507-001 White River RL0009 GTAAGT  OtsWENwr07-071507-002 White River RL0009 TCAAAG  OtsWENwr07-072407-001 White River RL0009 TTATGA  OtsWENwr09-062509-002 White River RL0009 TCGATT  OtsWENwr09-063009-001 White River RL0009 TTCTAG  OtsWENwr09-070209-001 White River RL0009 ACATAC