title: GSM3557123: TetTKO-B21-RNAseq-rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of cells was isolated using RNeasy Mini Kit (Qiagen), and cDNA were sythesised using PrimeScript first strand cDNA synthesis kit (Takara) with oligo-dT. ChIP-seq were performed as previously described (Xiong et al, Molecular Cell 64, 913-925). P65 antibody (Cat# sc-372) was purchased from Santa Cruz. The ChIP-seq libraries were constructed using NEBNext DNA Sample Prep Master Mix (NEB).DNA libraries were subjected to size-selection using AMPure XP beads (Beckman Coulter) and PCR amplification (18-20 cycles for ChIP-seq DNA libraries). The RNA-seq libraries were constructed by BGI, Shenzhen, as the platform BGISEQ-500 instruction described.
attributes.tag: GEO Accession
attributes.value: GSM3557123