title: GSM2337944: MCF-10A H3K4me3 ChIPseq biological replicate 2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP was performed on subconfluent cells as previously described (Lee et al., 2006) with the following modification: after sonication, buffer C was exchanged for FA buffer (50 mM HEPES-KOH, pH 7.5, 140 mM sodium chloride, 1 mM EDTA, 1% v/v Triton X-100, 0.1% w/v sodium deoxycholate, 0.1% w/v SDS, 1X Roche Complete EDTA-free protease inhibitor cocktail, 25 nM MG132 in nuclease-free water) prior to antibody pulldown using Amicon columns (10,000 kDa MWCO). The following antibodies were used for ChIP-Seq: H3K4me3 (mouse monoclonal, abcam ab1012),  H3K27me3 (rabbit polyclonal, Millipore 07-449), H3K9me2 (mouse monoclonal, abcam ab1220). Two  replicates were processed, normalized and the data combined for further analyses. After genomic DNA was isolated for both specific IPs and input DNA, libraries were prepared for paired-end multiplexed tag Solexa/Illumina sequencing using the Illumina DNA Sample Prep Kit v2.0 according to manufacturer instructions.
attributes.tag: GEO Accession
attributes.value: GSM2337944