title: PRO-seq human kidney
design: PRO-seq library generated from HK2 proximal tuble cells. Biotin-labelled nacent RNA converted to cDNA and the product was amplified 22 cycles. Products between 150-300 bp (insert >70 bp) were PAGE purified then sequenced by the Illumina HiSeq 2500 instrument to a depth of >150 million reads.