title: iPSC RNA: Sample 4388f90c-511f-4ffb-81d9-6f21f04df591
design: We isolated total RNA from total-cell lysates using the Quick-RNA™ MiniPrep Kit (Zymo Research) from frozen total-cell lysate material pellets, including on-column DNAse treatment steps and eluted in 60 µl RNAse-free water. RNA elutions were run on a Bioanalyzer (Agilent) to determine integrity and all samples had RNA integrity number (RIN) values greater than 9. Illumina Truseq Stranded mRNA libraries were prepared and sequenced on HiSeq4000, to an average of 28 M 125 bp paired-end reads per sample. 
attributes.tag: alignment_software
attributes.value: STAR