title: RAD-Seq in O. tshawytscha from Eel River, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsEel00-049 Eel River RL-0037 AAACGG  OtsEel00-051 Eel River RL-0037 ACTCTT  OtsEel00-053 Eel River RL-0037 ATTCCG  OtsEel00-054 Eel River RL-0037 CGAGGC  OtsEel00-055 Eel River RL-0037 GAAATG  OtsEel00-059 Eel River RL-0037 GGACTT  OtsEel00-060 Eel River RL-0037 TAAGCT  OtsEel00-061 Eel River RL-0037 TCTTCT  OtsEel00-062 Eel River RL-0037 AACGTT  OtsEel00-063 Eel River RL-0037 ACTGGC  OtsEel00-066 Eel River RL-0037 CAAAAA  OtsEel00-067 Eel River RL-0037 CGCAGA  OtsEel00-069 Eel River RL-0037 GAACCA  OtsEel00-070 Eel River RL-0037 GGCAAG  OtsEel00-071 Eel River RL-0037 TAATTC  OtsEel00-078 Eel River RL-0037 TGAACC  OtsEel00-081 Eel River RL-0037 AACTGA  OtsEel00-082 Eel River RL-0037 AGCCAT  OtsEel00-083 Eel River RL-0037 CAATCG  OtsEel00-084 Eel River RL-0037 CGCGTG  OtsEel00-087 Eel River RL-0037 GACGAC  OtsEel00-092 Eel River RL-0037 GGGCGC  OtsEel00-098 Eel River RL-0037 TACACA  OtsEel00-101 Eel River RL-0037 TGACAA  OtsEel00-105 Eel River RL-0037 AAGACG  OtsEel00-107 Eel River RL-0037 AGCGCA  OtsEel00-109 Eel River RL-0037 CACCTC  OtsEel00-117 Eel River RL-0037 CGGTCC  OtsEel00-118 Eel River RL-0037 GACTCT  OtsEel00-119 Eel River RL-0037 GGGGCG  OtsEel00-120 Eel River RL-0037 TACGGG  OtsEel00-121 Eel River RL-0037 TGCCCG  OtsEel00-122 Eel River RL-0037 AAGCTA  OtsEel00-123 Eel River RL-0037 AGGGTC  OtsEel00-127 Eel River RL-0037 CAGGCA  OtsEel00-128 Eel River RL-0037 CGTCTA  OtsEel00-129 Eel River RL-0037 GAGAGA  OtsEel00-130 Eel River RL-0037 GGTACA  OtsEel00-132 Eel River RL-0037 TAGTAT  OtsEel00-133 Eel River RL-0037 TGCTTA  OtsEel00-135 Eel River RL-0037 AATATC  OtsEel00-136 Eel River RL-0037 AGGTGT  OtsEel00-137 Eel River RL-0037 CATACT  OtsEel00-138 Eel River RL-0037 CGTGAT  OtsEel00-139 Eel River RL-0037 GATCGT  OtsEel00-141 Eel River RL-0037 GGTTTG  OtsEel00-143 Eel River RL-0037 TATCAC  OtsEel00-144 Eel River RL-0037 TGGGGA