title: GSM3936978: Ground_R9wt_D1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from each cell sample was extracted by using TRIzol reagent following the manufacturer's instructions. Libraries were constructed using Illumina mRNA-Seq library preparation kits. The concentration and size distribution of the libraries were determined by using an ABI 7500 instrument and an Agilent Bioanalyzer DNA 2000 chip (Agilent Technologies, Santa Clara, CA, USA), respectively. Qualified libraries were subjected to sequencing on an Illumina HiSeq 3000 Genome Analyzer platform with 2 ´ 100-bp paired-end reads.
attributes.tag: GEO Accession
attributes.value: GSM3936978