title: GSM3168879: WTA_rep1; Mus musculus; RNA-Seq
library_construction_protocol: mRNA-seq library preparation from 4 μg of total RNA was performed with TruSeq RNA Sample Prep Kit V2 (Illumina) according to the manufacturer's instructions. RNA-seq was carried out using the SMART-seq2 protocol (PMID:24385147) with minor modifications. Briefly, the poly-A containing mRNA molecules from 5 ng of total RNA were copied into first strand cDNA by reverse transcription and template-switching using oligo(dT) primers and an LNA-containing template-switching oligo (TSO). The resulting cDNA was pre-amplified, purified and tagmented with Tn5 transposase produced in-house using a described protocol (PMID:25079858). cDNA fragments generated after tagmentation were gap-repaired, enriched by PCR and purified to create the final cDNA library.
attributes.tag: GEO Accession
attributes.value: GSM3168879