title: 16S rRNA sequencing of maize C2 rhizosphere of crown root
design: Different maize genotypes were sown in the phytochamber with low nitrogen natural soil for 4 weeks and then rhizosphere soil from the 2nd whorl of crown root were dissected into three zones corresponding to root developmental zones. The genomic DNA was isolated from those rhizosphere soil and subjected to 16S rRNA gene V4 sequencing.