title: GSM3618095: small RNA-Seq, mESC, WT; Mus musculus; miRNA-Seq
library_construction_protocol: RNA-Seq: RNA was extracted from cell lines by using TRIzol reagent (Life technologies), following manufacturer's instructions. All RNA samples were subjected to DNAse I treatment (Ambion). RNA-Seq: RNA sequencing was performed following the protocol described by the Broad Institute (Cambridge, MA). Briefly, poly-A-plus mRNA, was retro-transcribed to cDNA using a strand specific dUTP method, random hexamers and amplified by PCR using bar-coded DNA adaptors from Illumina. HiSeq2000 platform and 50bp pair-end (PE) was used for sequencing.    Small RNA-Seq: library preparation was obtained using the Illumina TruSeq Small RNA protocol (Illumina) according to manufacturer instructions and libraries were sequenced using single-end (SE), 50bp reads on HiSeq2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM3618095