title: ITS rDNA_Seq: QC  bulk soil
design: The total  community DNA was extracted from soils  by soil DNA extraction kit. The ITS region was amplified in triplicate using  the barcoded ITS1F and 58A2R primer  set. PCR products were quantified, purified and sequenced by Illumina Miseq  platform for assessment of fungal diversity and community structure.