title: Sepia esculenta sucker ring transcriptome
design: Total RNA was extracted from a whole sucker ring tissue of a Sepia esculenta  individual with a Qiagen RNeasy mini kit. 2–10 μg Total RNA per tissue was used in the construction of three separate RNA-seq libraries. Diethylpyrocarbonate (DEPC) treated water was added to total RNA to a final 50 μl volume and incubated at 65 *C for 5 min. Poly-A mRNA was enriched with oligo dT beads (Invitrogen) and used for constructing strand-specific paired-end libraries (ScriptSeq mRNA-Seq library kit v1, Epicenter, Illumina). Phusion PCR polymerase (Thermo Scientific) was used for the final library amplification (12 cycles). PCR cleanup was done with the MinElute PCR purification kit (Qiagen). Library quantity and quality were assessed with an Agilent 2100 Bioanalyzer.