title: GSM3754096: nATF61: 5 dpf Livers of ATF6 transgenic zebrafish-1; Danio rerio; RNA-Seq
library_construction_protocol: Tg(fabp10:nAtf6-cherry; cmlc2:GFP)C+/, were outcrossed to WT TAB 14 and the embryos were sorted and segregated basing on the expression of Cmlc2:GFP. The three different clutches of embryos were collected and dissected over three different days. The embryos were collected inbetween 10- 11 AM. The Liver dissection was also performed on the 5dpf larvae before noon. 25 livers from 5dpf embryos were pooled per sample( Transgenic and WT siblings in two different tubes) and RNA extracted with Trizol as per manufacturer's instructions.The RNA was later treated with DNAse to remove any DNA. They RNA concentration was taken on the Q bit, and then analysed on the Bioanalyser for the integrety and quality of RNA. The RIN score for the samples was above 8.5 RNA-seq libraries were prepared according to Illumina TruSeq RNA sample preparation version 2 protocol with Ribo-Zero Gold (Catalog #: RS-122-2501)
attributes.tag: GEO Accession
attributes.value: GSM3754096