title: GSM4677566: h3k27me3_ring1ab_1; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Chromatin was extracted from 10-day-old whole seedlings. Anti-H2Aub (Cell Signaling Technology, 8240S) and anti-H3K27me3 (Millipore, 07-449) antibodies were used for chromatin immunoprecipitation. Libraries were prepared with end repair, A-tailing and ligation of Illumina-compatible adapters using the Ovation® Ultralow Library Systems (NuGEN). The ligated product was amplified with 14 cycles of PCR. DNA of a size range between 200 and 600 bp was purified from an agarose gel. Amplification was confirmed by testing an aliquot of the library before and after amplification by qPCR.
attributes.tag: GEO Accession
attributes.value: GSM4677566