title: RNA-Seq of grapevine roots: alkali conditions
design: Total RNA of 10-mm-long grapevine roots from the apex was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and mRNA was isolated using poly-T oligo attached magnetic beads. The transcriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China). Sequencing libraries, constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (#7530L, NEB, United States)