title: RAD-Seq in O. tshawytscha from Johnson Creek, ID
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsJCP08-1224 Johnson Creek RL-0040 AAACGG  OtsJCP08-1227 Johnson Creek RL-0040 ACTCTT  OtsJCP08-1229 Johnson Creek RL-0040 ATTCCG  OtsJCP08-1231 Johnson Creek RL-0040 CGAGGC  OtsJCP08-1235 Johnson Creek RL-0040 GAAATG  OtsJCP08-1238 Johnson Creek RL-0040 GGACTT  OtsJCP08-1247 Johnson Creek RL-0040 TAAGCT  OtsJCP08-1253 Johnson Creek RL-0040 TCTTCT  OtsJCP08-1255 Johnson Creek RL-0040 AACGTT  OtsJCP08-1256 Johnson Creek RL-0040 ACTGGC  OtsJCP08-1262 Johnson Creek RL-0040 CAAAAA  OtsJCP08-1271 Johnson Creek RL-0040 CGCAGA  OtsJCP08-1278 Johnson Creek RL-0040 GAACCA  OtsJCP08-1282 Johnson Creek RL-0040 GGCAAG  OtsJCP08-1283 Johnson Creek RL-0040 TAATTC  OtsJCP08-1287 Johnson Creek RL-0040 TGAACC  OtsJCP08-1288 Johnson Creek RL-0040 AACTGA  OtsJCP08-1290 Johnson Creek RL-0040 AGCCAT  OtsJCP08-1292 Johnson Creek RL-0040 CAATCG  OtsJCP08-1294 Johnson Creek RL-0040 CGCGTG  OtsJCP08-1296 Johnson Creek RL-0040 GACGAC  OtsJCP08-1297 Johnson Creek RL-0040 GGGCGC  OtsJCP08-1298 Johnson Creek RL-0040 TACACA  OtsJCP08-1299 Johnson Creek RL-0040 TGACAA  OtsJCP08-1300 Johnson Creek RL-0040 AAGACG  OtsJCP08-1307 Johnson Creek RL-0040 AGCGCA  OtsJCP08-1312 Johnson Creek RL-0040 CACCTC  OtsJCP08-1313 Johnson Creek RL-0040 CGGTCC  OtsJCP08-1315 Johnson Creek RL-0040 GACTCT  OtsJCP08-1320 Johnson Creek RL-0040 GGGGCG  OtsJCP08-1321 Johnson Creek RL-0040 TACGGG  OtsJCP08-1325 Johnson Creek RL-0040 TGCCCG  OtsJCP08-1326 Johnson Creek RL-0040 AAGCTA  OtsJCP08-1341 Johnson Creek RL-0040 AGGGTC  OtsJCP08-1342 Johnson Creek RL-0040 CAGGCA  OtsJCP08-1344 Johnson Creek RL-0040 CGTCTA  OtsJCP08-1346 Johnson Creek RL-0040 GAGAGA  OtsJCP08-1349 Johnson Creek RL-0040 GGTACA  OtsJCP08-1352 Johnson Creek RL-0040 TAGTAT  OtsJCP08-1353 Johnson Creek RL-0040 TGCTTA  OtsJCP08-1358 Johnson Creek RL-0040 AATATC  OtsJCP08-1360 Johnson Creek RL-0040 AGGTGT  OtsJCP08-1363 Johnson Creek RL-0040 CATACT  OtsJCP08-1365 Johnson Creek RL-0040 CGTGAT  OtsJCP08-1371 Johnson Creek RL-0040 GATCGT  OtsJCP08-1372 Johnson Creek RL-0040 GGTTTG  OtsJCP08-1374 Johnson Creek RL-0040 TATCAC  OtsJCP08-1376 Johnson Creek RL-0040 TGGGGA