title: GSM2333830: NAF control-2; Homo sapiens; RNA-Seq
library_construction_protocol: samples were collected in Trizol and total RNA was extracted using the RNeasy Mini Kit (Qiagen). RNA concentration and quality were tested respectively with Nanodrop 8000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent) 1 μg of total RNA per sample was used for library construction with the Illumina TruSeq stranded mRNA library preparation kit. Briefly streptavidin-coated magnetic beads were employed for poly-A mRNA selection and cDNA was synthesized from enriched and fragmented RNA using reverse transcriptase (Super-Script II, Invitrogen) and random primers. After conversion into double stranded DNA and PCR amplification the products were enriched for library preparation and purified using Agencourt AMPure XP beads (Beckman Coulter Genomics).
attributes.tag: GEO Accession
attributes.value: GSM2333830