title: GSM3018846: HC178; Homo sapiens; RNA-Seq
library_construction_protocol: Neutrophils were purified from whole blood using Ficoll-Hypaque gradient centrifugation. Cells were lysed in TRI Reagent (Sigma Aldrich), and RNA was extracted following the manufacturer's protocol. RNA quality was assessed using RNA electrophoresis (Agilent Bioanalyze 2100). All samples had RIN values >7. Sequencing libraries were constructed from total RNA using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB Biolabs), followed by purification with AMPure XP Beads (Beckman Coulter)
attributes.tag: GEO Accession
attributes.value: GSM3018846