title: RAD-Seq in O. tshawytscha from McCall Fish Hatchery, ID & Quinault Lake Pens, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10NC0001 McCall Hatchery - South Fork Salmon River RL-0035 AAACGG  OtsCORE-10NC0002 McCall Hatchery - South Fork Salmon River RL-0035 ACTCTT  OtsCORE-10NC0003 McCall Hatchery - South Fork Salmon River RL-0035 ATTCCG  OtsCORE-10NC0004 McCall Hatchery - South Fork Salmon River RL-0035 CGAGGC  OtsCORE-10NC0005 McCall Hatchery - South Fork Salmon River RL-0035 GAAATG  OtsCORE-10NC0006 McCall Hatchery - South Fork Salmon River RL-0035 GGACTT  OtsCORE-10NC0007 McCall Hatchery - South Fork Salmon River RL-0035 TAAGCT  OtsCORE-10NC0008 McCall Hatchery - South Fork Salmon River RL-0035 TCTTCT  OtsCORE-10NC0009 McCall Hatchery - South Fork Salmon River RL-0035 AACGTT  OtsCORE-10NC0010 McCall Hatchery - South Fork Salmon River RL-0035 ACTGGC  OtsCORE-10NC0011 McCall Hatchery - South Fork Salmon River RL-0035 CAAAAA  OtsCORE-10NC0012 McCall Hatchery - South Fork Salmon River RL-0035 CGCAGA  OtsCORE-10NC0013 McCall Hatchery - South Fork Salmon River RL-0035 GAACCA  OtsCORE-10NC0014 McCall Hatchery - South Fork Salmon River RL-0035 GGCAAG  OtsCORE-10NC0015 McCall Hatchery - South Fork Salmon River RL-0035 TAATTC  OtsCORE-10NC0016 McCall Hatchery - South Fork Salmon River RL-0035 TGAACC  OtsCORE-10NC0017 McCall Hatchery - South Fork Salmon River RL-0035 AACTGA  OtsCORE-10NC0018 McCall Hatchery - South Fork Salmon River RL-0035 AGCCAT  OtsCORE-10NC0019 McCall Hatchery - South Fork Salmon River RL-0035 CAATCG  OtsCORE-10NC0020 McCall Hatchery - South Fork Salmon River RL-0035 CGCGTG  OtsCORE-10NC0021 McCall Hatchery - South Fork Salmon River RL-0035 GACGAC  OtsCORE-10NC0022 McCall Hatchery - South Fork Salmon River RL-0035 GGGCGC  OtsCORE-10NC0023 McCall Hatchery - South Fork Salmon River RL-0035 TACACA  OtsCORE-10NC0024 McCall Hatchery - South Fork Salmon River RL-0035 TGACAA  OtsCORE-10NC0025 McCall Hatchery - South Fork Salmon River RL-0035 AAGACG  OtsCORE-10NC0026 McCall Hatchery - South Fork Salmon River RL-0035 AGCGCA  OtsCORE-10NC0027 McCall Hatchery - South Fork Salmon River RL-0035 CACCTC  OtsCORE-10NC0028 McCall Hatchery - South Fork Salmon River RL-0035 CGGTCC  OtsCORE-10NC0029 McCall Hatchery - South Fork Salmon River RL-0035 GACTCT  OtsCORE-10NC0030 McCall Hatchery - South Fork Salmon River RL-0035 GGGGCG  OtsCORE-10NC0031 McCall Hatchery - South Fork Salmon River RL-0035 TACGGG  OtsCORE-10NC0032 McCall Hatchery - South Fork Salmon River RL-0035 TGCCCG  OtsCORE-10NC0033 McCall Hatchery - South Fork Salmon River RL-0035 AAGCTA  OtsCORE-10NC0034 McCall Hatchery - South Fork Salmon River RL-0035 AGGGTC  OtsCORE-10NC0035 McCall Hatchery - South Fork Salmon River RL-0035 CAGGCA  OtsCORE-10NC0036 McCall Hatchery - South Fork Salmon River RL-0035 CGTCTA  OtsCORE-10NC0038 McCall Hatchery - South Fork Salmon River RL-0035 GGTACA  OtsCORE-10NC0039 McCall Hatchery - South Fork Salmon River RL-0035 TAGTAT  OtsCORE-10NC0040 McCall Hatchery - South Fork Salmon River RL-0035 TGCTTA  OtsCORE-10NC0041 McCall Hatchery - South Fork Salmon River RL-0035 AATATC  OtsCORE-10NC0042 McCall Hatchery - South Fork Salmon River RL-0035 AGGTGT  OtsCORE-10NC0043 McCall Hatchery - South Fork Salmon River RL-0035 CATACT  OtsCORE-10NC0044 McCall Hatchery - South Fork Salmon River RL-0035 CGTGAT  OtsCORE-10NC0045 McCall Hatchery - South Fork Salmon River RL-0035 GATCGT  OtsCORE-10NC0046 McCall Hatchery - South Fork Salmon River RL-0035 GGTTTG  OtsCORE-10NC0047 McCall Hatchery - South Fork Salmon River RL-0035 TATCAC  OtsCORE-10NC0048 McCall Hatchery - South Fork Salmon River RL-0035 TGGGGA  OtsCORE-10NJ0001 Quinault Lake Hatchery and Net Pens RL-0035 AATGAG  OtsCORE-10NJ0002 Quinault Lake Hatchery and Net Pens RL-0035 AGTAGG  OtsCORE-10NJ0003 Quinault Lake Hatchery and Net Pens RL-0035 CCATTT  OtsCORE-10NJ0004 Quinault Lake Hatchery and Net Pens RL-0035 CTACAG  OtsCORE-10NJ0005 Quinault Lake Hatchery and Net Pens RL-0035 GCAGAT  OtsCORE-10NJ0006 Quinault Lake Hatchery and Net Pens RL-0035 GTAAGT  OtsCORE-10NJ0007 Quinault Lake Hatchery and Net Pens RL-0035 TCAAAG  OtsCORE-10NJ0008 Quinault Lake Hatchery and Net Pens RL-0035 TTATGA  OtsCORE-10NJ0009 Quinault Lake Hatchery and Net Pens RL-0035 ACAAGA  OtsCORE-10NJ0010 Quinault Lake Hatchery and Net Pens RL-0035 AGTTAA  OtsCORE-10NJ0011 Quinault Lake Hatchery and Net Pens RL-0035 CCCGGT  OtsCORE-10NJ0012 Quinault Lake Hatchery and Net Pens RL-0035 CTCGCC  OtsCORE-10NJ0013 Quinault Lake Hatchery and Net Pens RL-0035 GCATGG  OtsCORE-10NJ0014 Quinault Lake Hatchery and Net Pens RL-0035 GTATCC  OtsCORE-10NJ0015 Quinault Lake Hatchery and Net Pens RL-0035 TCCTGC  OtsCORE-10NJ0016 Quinault Lake Hatchery and Net Pens RL-0035 TTCCGT  OtsCORE-10NJ0051 Quinault Lake Hatchery and Net Pens RL-0035 ACAGCG  OtsCORE-10NJ0052 Quinault Lake Hatchery and Net Pens RL-0035 ATAGTA  OtsCORE-10NJ0053 Quinault Lake Hatchery and Net Pens RL-0035 CCCTAA  OtsCORE-10NJ0054 Quinault Lake Hatchery and Net Pens RL-0035 CTGCGA  OtsCORE-10NJ0055 Quinault Lake Hatchery and Net Pens RL-0035 GCCGTA  OtsCORE-10NJ0056 Quinault Lake Hatchery and Net Pens RL-0035 GTCATC  OtsCORE-10NJ0057 Quinault Lake Hatchery and Net Pens RL-0035 TCGATT  OtsCORE-10NJ0058 Quinault Lake Hatchery and Net Pens RL-0035 TTCTAG  OtsCORE-10NJ0059 Quinault Lake Hatchery and Net Pens RL-0035 ACATAC  OtsCORE-10NJ0060 Quinault Lake Hatchery and Net Pens RL-0035 ATCAAA  OtsCORE-10NJ0061 Quinault Lake Hatchery and Net Pens RL-0035 CCGAGG  OtsCORE-10NJ0062 Quinault Lake Hatchery and Net Pens RL-0035 CTGGTT  OtsCORE-10NJ0063 Quinault Lake Hatchery and Net Pens RL-0035 GCGACC  OtsCORE-10NJ0064 Quinault Lake Hatchery and Net Pens RL-0035 GTGCCT  OtsCORE-10NJ0065 Quinault Lake Hatchery and Net Pens RL-0035 TCGCCA  OtsCORE-10NJ0066 Quinault Lake Hatchery and Net Pens RL-0035 TTGAGC  OtsCORE-10NJ0076 Quinault Lake Hatchery and Net Pens RL-0035 ACCATG  OtsCORE-10NJ0077 Quinault Lake Hatchery and Net Pens RL-0035 ATGCAC  OtsCORE-10NJ0078 Quinault Lake Hatchery and Net Pens RL-0035 CCGCAT  OtsCORE-10NJ0079 Quinault Lake Hatchery and Net Pens RL-0035 CTTATG  OtsCORE-10NJ0080 Quinault Lake Hatchery and Net Pens RL-0035 GCGCTG  OtsCORE-10NJ0081 Quinault Lake Hatchery and Net Pens RL-0035 GTGTAA  OtsCORE-10NJ0082 Quinault Lake Hatchery and Net Pens RL-0035 TCGGAC  OtsCORE-10NJ0083 Quinault Lake Hatchery and Net Pens RL-0035 TTTAAT  OtsCORE-10NJ0086 Quinault Lake Hatchery and Net Pens RL-0035 ACCCCC  OtsCORE-10NJ0087 Quinault Lake Hatchery and Net Pens RL-0035 ATGTTG  OtsCORE-10NJ0088 Quinault Lake Hatchery and Net Pens RL-0035 CCTAAC  OtsCORE-10NJ0089 Quinault Lake Hatchery and Net Pens RL-0035 CTTTGC  OtsCORE-10NJ0090 Quinault Lake Hatchery and Net Pens RL-0035 GCTCAA  OtsCORE-10NJ0091 Quinault Lake Hatchery and Net Pens RL-0035 GTTGGA  OtsCORE-10NJ0092 Quinault Lake Hatchery and Net Pens RL-0035 TCTCGG  OtsCORE-10NJ0093 Quinault Lake Hatchery and Net Pens RL-0035 TTTGTC