title: miRNA-Seq of liver: 10 weeks old female chicken
design: Small RNA was extracted from the chicken liver tissues using mirVana  miRNA Isolation Kit Cat # AM1561, Austin TX, US following the manufacturer s manual. The extracted RNAs were then ligated sequentially with 3  and 5  RNA adapters by T4 RNA ligase (Cat#M0242l, BioLabs). The ligated RNA samples were reverse-transcribed and amplified by PCR to produce cDNA. Subsequently, cDNA with appropriate lengths were purified from agarose gel to construct the sequence libraries. After purification, the small RNA libraries were quantified with Qubit Fluorometer (Invitrogen) and then used for cluster generation and 50bp single end sequencing analysis with Illumina HiSeq 4000 system according to the manufacturer s instructions.