title: Mycobacterium bovis isolated from bison
design: Mycobacterium bovis isolates from Bison bonasus was used. These isolates were propagated in Stonebrink media. One colony was collected and inoculated in Stonebrink to guarantee clonality and its DNA was extracted. The quality and quantity of the DNA was measured by Nanodrop 2000c (Thermo Scientific, MA, USA). The DNA was then quantified on 0.8% agarose gel with molecular weight marker and analyzed on DNA High Sensitivity Chip from Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) for checking the concentration and fragmentation of the sample. The genomic libraries of each isolate were already constructed in the paired-end model with the TruSeq DNA PCR-free sample preparation kit (Illumina, CA, USA) according to the manufacturer's instructions. The library was sequenced on HiSeq2500 platform (Illumina v3 chemistry, 2 x 100bp), at LaCTAD, University of Campinas, Brazil.