title: GSM3019092: 3362-ACP-0012; Homo sapiens; RNA-Seq
library_construction_protocol: RNAqueous Micro-Total RNA Isolation Kit (Ambion/Invitrogen). Trace contaminating DNA was removed with TURBO DNA-free (Ambion/Invitrogen). Both murine and human islets were handpicked under microscope guidance, washed with PBS, and immediately frozen at -80 ºC. Islet RNA was extracted using the RNAqueous Micro-Total RNA Isolation Kit (Ambion/Invitrogen). Trace contaminating DNA was removed with TURBO DNA-free (Ambion/Invitrogen). RNA quality control quantification was performed using a Qubit Fluorometer (Invitrogen) and an Agilent 2100 Bioanalyzer. All RNA samples had an RNA integrity number (RIN) ≥6.0. RNA was amplified using the NuGEN Technologies Ovation RNA amplification kit optimized for RNA sequencing. Following amplification, the resulting cDNA was sheared to an average insert size of 300bp and used for library preparation. Sequencing was performed using standard Illumina methods.
attributes.tag: GEO Accession
attributes.value: GSM3019092