title: RNA-Seq of GnRH agonist treated cell lines from premenstrual dysphoric disorder patients and controls: Sample S26
design: Total RNA was isolated from lymphoblastoid cell line cultures cell pellets using TRIzol reagents (Life Technologies, Cat. No. 15596018) for cDNA library construction . The RNA samples were depleted of ribosomal RNA by two rounds of passage of 10 μg of total RNA using the RiboMinus Eukaryote Kit for RNA (Invitrogen, Cat. No. A1083708), and the RiboMinus Concentration Module (Invitrogen, Cat. No. K1550-05). 300 ng rRNA-depleted total RNA per sample was treated with RNase III to generate 100- to 200-nt fragments. The RNA fragments were hybridized, ligated with Ambion Adaptor Mix, reverse-transcribed, size-selected, amplified, and purified using the Ambion RNA-seq library construction kit (Invitrogen, Cat No. 4454073).