title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of C17.2 neuronal progenitor cells exposed to cerium oxide nanoparticles during cell differentiation
design: RNA-seq of C17.2 neuronal progenitor cells exposed to cerium oxide nanoparticles during cell differentiation
library_construction_protocol: Under proliferating conditions the C17.2 cells were grown in cell culture dishes in DMEM high glucose, GlutaMAX (Gibco) supplemented with 5% horse serum, heat inactivated (Gibco) and 10% fetal bovine serum, heat inactivated (Gibco), 2 mM glutamine (Gibco), 100 U penicillin/mL, and 100 U streptomycin/mL (Gibco). The cells were split every 3-4 days. During this proliferating phase samples were harvested for RNAseq. For RNA extraction, the cells were washed after exposure and total RNA was extracted using the RNeasy Mini Columns (Qiagen) according to the manufacturers instructions, including the purification step with DNase I treatment.  Stranded mRNA-seq (Illumina TruSeq RNA HT with polyA-selection
attributes.tag: Experimental Factor: none
attributes.value: compound
attributes.tag: Experimental Factor: undifferentiated
attributes.value: developmental stage