title: SMRT-seq of rAAV preparation: scAAV9-CB-PI-H1-Apob-R-EGFP
design: Purified viral vectors were generated, purified, and titrated as described in Gao, G. & Sena-Esteves, M. in Molecular Cloning: A Laboratory Manual Vol. 2 1209-1313 (Cold Spring Harbor Laboratory Press, 2012).  Virions were digested with DNaseI and viral DNAs were extracted following procedures for extraction of recombinant adenovirus genomic DNA in Gao et al.  DNA from purified rAAV preparations was spiked with 10% _DNA digested by BstEII for normalization. DNAs were subjected to DNA nick repair, followed by direct ligation to SMRTbell adapters at a 1:1, adapter to vector molecular ratio and sequenced on a Pacific Biosciences RSII.