title: RAD-Seq in O. tshawytscha from Togiak River weir, AK
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-09NA0001 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AAACGG  OtsCORE-09NA0002 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 ACTCTT  OtsCORE-09NA0006 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 ATTCCG  OtsCORE-09NA0007 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CGAGGC  OtsCORE-09NA0008 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GAAATG  OtsCORE-09NA0010 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GGACTT  OtsCORE-09NA0012 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TAAGCT  OtsCORE-09NA0013 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TCTTCT  OtsCORE-09NA0018 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AACGTT  OtsCORE-09NA0019 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 ACTGGC  OtsCORE-09NA0025 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CAAAAA  OtsCORE-09NA0027 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CGCAGA  OtsCORE-09NA0028 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GAACCA  OtsCORE-09NA0030 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GGCAAG  OtsCORE-09NA0034 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TAATTC  OtsCORE-09NA0036 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TGAACC  OtsCORE-09NA0037 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AACTGA  OtsCORE-09NA0040 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AGCCAT  OtsCORE-09NA0041 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CAATCG  OtsCORE-09NA0042 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CGCGTG  OtsCORE-09NA0045 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GACGAC  OtsCORE-09NA0046 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GGGCGC  OtsCORE-09NA0047 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TACACA  OtsCORE-09NA0048 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TGACAA  OtsCORE-09NA0049 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AAGACG  OtsCORE-09NA0050 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AGCGCA  OtsCORE-09NA0051 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CACCTC  OtsCORE-09NA0052 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CGGTCC  OtsCORE-09NA0053 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GACTCT  OtsCORE-09NA0054 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GGGGCG  OtsCORE-09NA0055 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TACGGG  OtsCORE-09NA0058 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TGCCCG  OtsCORE-09NA0060 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AAGCTA  OtsCORE-09NA0063 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AGGGTC  OtsCORE-09NA0066 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CAGGCA  OtsCORE-09NA0068 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CGTCTA  OtsCORE-09NA0070 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GAGAGA  OtsCORE-09NA0071 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GGTACA  OtsCORE-09NA0072 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TAGTAT  OtsCORE-09NA0074 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TGCTTA  OtsCORE-09NA0077 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AATATC  OtsCORE-09NA0078 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 AGGTGT  OtsCORE-09NA0079 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CATACT  OtsCORE-09NA0080 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 CGTGAT  OtsCORE-09NA0081 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GATCGT  OtsCORE-09NA0083 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 GGTTTG  OtsCORE-09NA0084 Togiak River weir - Togiak Drainage - Bristol Bay RL-0057 TTTGTC