title: GSM2338124: LM_HN_intact_RNA seq; Sus scrofa; RNA-Seq
library_construction_protocol: LM were removed from HN and DLY pigs, frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. The ribosomal RNA (rRNA) was removed using Ribo-ZeroTM kit (Epicentre, Madison, WY, USA) from the DNA-free RNA. Then, the DNA and rRNA-free RNA was used to prepare the library using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs lnc., Beverly, MA), and then sequenced by HiSeq 2500 (Illumina, San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM2338124