title: qiime_ppdid_136:10058.BLANK.41.8B
design: stool samples from American gut Project
library_construction_protocol: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: barcodesequence
attributes.value: CCAATACGCCTG
attributes.tag: center_name
attributes.value: CCME
attributes.tag: center_project_name
attributes.value: American gut 15
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: experiment_center
attributes.value: CCME
attributes.tag: experiment_design_description
attributes.value: stool samples from American gut Project
attributes.tag: experiment_title
attributes.value: American Gut 15
attributes.tag: extraction_robot
attributes.value: unknown
attributes.tag: extractionkit_lot
attributes.value: unknown
attributes.tag: file_path
attributes.value: /projects/qiita_data/working_dir/vpPo9i/10058.BLANK.41.8B.fastq.gz
attributes.tag: file_type
attributes.value: fastq
attributes.tag: illumina_technology
attributes.value: MiSeq
attributes.tag: library_construction_protocol
attributes.value: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: linker
attributes.value: GT
attributes.tag: linkerprimersequence
attributes.value: GTGCCAGCMGCCGCGGTAA
attributes.tag: mastermix_lot
attributes.value: unknown
attributes.tag: orig_name
attributes.value: 17327
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: platform
attributes.value: Illumina
attributes.tag: plating
attributes.value: unknown
attributes.tag: primer_date
attributes.value: unknown
attributes.tag: primer_plate
attributes.value: 3
attributes.tag: processing_robot
attributes.value: unknown
attributes.tag: project_name
attributes.value: AGP15
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 8/21/14
attributes.tag: run_prefix
attributes.value: Undetermined_S0_L001
attributes.tag: samp_size
attributes.value: .1,g
attributes.tag: sample_center
attributes.value: CCME
attributes.tag: sample_plate
attributes.value: AG_53
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: tm1000_8_tool
attributes.value: unknown
attributes.tag: tm300_8_tool
attributes.value: unknown
attributes.tag: tm50_8_tool
attributes.value: unknown
attributes.tag: water_lot
attributes.value: unknown
attributes.tag: well_id
attributes.value: H9