title: Virginia Single Population Campanulastrum americanum chloroplast sequencing
design: Virginia Campanulastrum americanum plants from a single maternal family were grown, and chloroplasts were isolated from leaf tissue. Chloroplasts were lysed and DNA was obtained via a phenol-chloroform extraction and ethanol precipitation. A shotgun library was constructed with a multiplex identifier (MID) tag following standard protocols for sequencing on a Roche 454 GS-FLX platform with Titanium reagents. The MID-tagged library was sequenced as part of a larger pooled sample in two separate sequencing runs.