title: GSM2577533: CD8 cells timestamped at day 28 rep 1; Mus musculus; RNA-Seq
library_construction_protocol: CD8+ cells were magnetically enriched with CD8 microbeads and sorted to > 95% purity with a FACS Aria III on CD4- CD8+ TdTomato+. Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation RNA-seq libraries were prepared from 50 ng total RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.
attributes.tag: GEO Accession
attributes.value: GSM2577533