title: GSM3754020: TDL_CXCR5Neg (E4) RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from sorted cells was extracted using the Applied Biosystems Arcturus PicoPure RNA isolation kit Double stranded cDNA was generated using the Clontech SMRT-seq v4 method and was fragmented using the Covaris S220 in microTubes. Indexed Illumina-compatible sequencing libraries were generated from fragmented cDNA using the NEBNext Ultra IImethodology Libraries were quantified using Kapa Library QC kit for Illumina, pooled, and sequenced on an Illumina NextSeq 500 for 75 cycles (single end)