title: Illumina HiSeq 2000 sequencing; qiita_ptid_77:678.OA.mesocosm.357
design: Samples taken from surface sediments and sediment from the burrow walls of Upogebia deltaura, a bioturbating shrimp species. Sediments were subjected to 5 different pH levels over a 14-week exposure period. Bacterial and archaeal 16S genes were sequenced.
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: GCCATAGTGTGT
attributes.tag: center_name
attributes.value: Plymouth Marine Laboratory
attributes.tag: center_project_name
attributes.value: Investigation into the effects of ocean acidification on microbial communities in bioturbated coastal sediments
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: HiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: orig_sample_name
attributes.value: 357
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: ANL
attributes.tag: run_date
attributes.value: 11/3/10
attributes.tag: run_prefix
attributes.value: Bonnie-Pool-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_5_1_sequence
attributes.tag: samp_size
attributes.value: .1,g
attributes.tag: sample_center
attributes.value: ANL
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4