title: RAD-Seq in O. tshawytscha from Cole M. Rivers Hatchery, OR & Cedar Creek Hatchery, Nestucca, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10OH0001 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 AATGAG  OtsCORE-10OH0002 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 AGTAGG  OtsCORE-10OH0003 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CCATTT  OtsCORE-10OH0004 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CTACAG  OtsCORE-10OH0005 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GCAGAT  OtsCORE-10OH0006 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GTAAGT  OtsCORE-10OH0007 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TCAAAG  OtsCORE-10OH0008 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TTATGA  OtsCORE-10OH0010 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ACAAGA  OtsCORE-10OH0011 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 AGTTAA  OtsCORE-10OH0013 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CCCGGT  OtsCORE-10OH0014 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CTCGCC  OtsCORE-10OH0015 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GCATGG  OtsCORE-10OH0016 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GTATCC  OtsCORE-10OH0017 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TCCTGC  OtsCORE-10OH0018 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TTCCGT  OtsCORE-10OH0019 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ACAGCG  OtsCORE-10OH0020 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ATAGTA  OtsCORE-10OH0021 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CCCTAA  OtsCORE-10OH0023 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CTGCGA  OtsCORE-10OH0024 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GCCGTA  OtsCORE-10OH0027 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GTCATC  OtsCORE-10OH0028 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TCGATT  OtsCORE-10OH0030 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TTCTAG  OtsCORE-10OH0031 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ACATAC  OtsCORE-10OH0033 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ATCAAA  OtsCORE-10OH0035 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CCGAGG  OtsCORE-10OH0037 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CTGGTT  OtsCORE-10OH0038 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GCGACC  OtsCORE-10OH0041 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GTGCCT  OtsCORE-10OH0042 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TCGCCA  OtsCORE-10OH0043 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TTGAGC  OtsCORE-10OH0044 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ACCATG  OtsCORE-10OH0046 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ATGCAC  OtsCORE-10OH0047 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CCGCAT  OtsCORE-10OH0052 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GTGTAA  OtsCORE-10OH0055 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TTTAAT  OtsCORE-10OH0057 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ACCCCC  OtsCORE-10OH0058 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 ATGTTG  OtsCORE-10OH0059 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CCTAAC  OtsCORE-10OH0060 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 CTTTGC  OtsCORE-10OH0061 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GCTCAA  OtsCORE-10OH0064 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 GTTGGA  OtsCORE-10OH0065 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TCTCGG  OtsCORE-10OH0069 Cedar Ck Hatchery. 33465 Hwy. 22. Hebo OR 97122 RL-0036 TTTGTC  OtsCORE-10NT0001 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AAACGG  OtsCORE-10NT0003 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 ACTCTT  OtsCORE-10NT0007 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CGAGGC  OtsCORE-10NT0008 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GAAATG  OtsCORE-10NT0009 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GGACTT  OtsCORE-10NT0012 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TAAGCT  OtsCORE-10NT0017 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TCTTCT  OtsCORE-10NT0019 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AACGTT  OtsCORE-10NT0023 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 ACTGGC  OtsCORE-10NT0025 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CAAAAA  OtsCORE-10NT0028 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CGCAGA  OtsCORE-10NT0029 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GAACCA  OtsCORE-10NT0031 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GGCAAG  OtsCORE-10NT0035 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TAATTC  OtsCORE-10NT0038 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TGAACC  OtsCORE-10NT0054 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AACTGA  OtsCORE-10NT0059 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AGCCAT  OtsCORE-10NT0061 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CAATCG  OtsCORE-10NT0062 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CGCGTG  OtsCORE-10NT0063 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GACGAC  OtsCORE-10NT0064 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GGGCGC  OtsCORE-10NT0070 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TACACA  OtsCORE-10NT0072 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TGACAA  OtsCORE-10NT0073 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AAGACG  OtsCORE-10NT0077 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AGCGCA  OtsCORE-10NT0078 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CACCTC  OtsCORE-10NT0082 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CGGTCC  OtsCORE-10NT0083 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GACTCT  OtsCORE-10NT0084 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GGGGCG  OtsCORE-10NT0087 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TACGGG  OtsCORE-10NT0090 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TGCCCG  OtsCORE-10NT0094 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AAGCTA  OtsCORE-10NT0095 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AGGGTC  OtsCORE-10NT0096 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CAGGCA  OtsCORE-10NT0098 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CGTCTA  OtsCORE-10NT0101 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GAGAGA  OtsCORE-10NT0109 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GGTACA  OtsCORE-10NT0115 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TAGTAT  OtsCORE-10NT0119 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AATATC  OtsCORE-10NT0120 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 AGGTGT  OtsCORE-10NT0122 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CATACT  OtsCORE-10NT0124 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 CGTGAT  OtsCORE-10NT0131 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GATCGT  OtsCORE-10NT0133 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 GGTTTG  OtsCORE-10NT0134 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TATCAC  OtsCORE-10NT0137 Cole Rivers Hatchery. 200 Cole M. Rivers Drive. Trail OR 97541 RL-0036 TGGGGA