title: GSM3753001: H3K4me3_TESm_1; Mus musculus; ChIP-Seq
library_construction_protocol: Embryonic gonads were micro-dissected at according stage, removed from the mesonephros and incubated in 0.05% trypsin for 5 minutes at 37C. Gonads were then mechanically dissasociated in PBS/3% BSA and passed through a filter cap to obtain a single cell suspension. Fluorescently-labeled cells were collected by Fluorescence Activated Cell Sorting (FACS).  For ChIP-seq, 30K-100K cells were collected per replicate. ChIP-seq libraries were prepared with no modifications according to Van Galen et al., 2016.