title: RAD-Seq in O. tshawytscha from Newsome Creek, ID
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsNEWS10-0019 South Fork Clearwater River - Newsome Creek RL-0039 TCGCCA  OtsNEWS10-0022 South Fork Clearwater River - Newsome Creek RL-0039 TTGAGC  OtsNEWS10-0031 South Fork Clearwater River - Newsome Creek RL-0039 ACCATG  OtsNEWS10-0040 South Fork Clearwater River - Newsome Creek RL-0039 ATGCAC  OtsNEWS10-0048 South Fork Clearwater River - Newsome Creek RL-0039 CCGCAT  OtsNEWS10-0083 South Fork Clearwater River - Newsome Creek RL-0039 CTTATG  OtsNEWS10-0100 South Fork Clearwater River - Newsome Creek RL-0039 GCGCTG  OtsNEWS10-0115 South Fork Clearwater River - Newsome Creek RL-0039 GTGTAA  OtsNEWS10-0166 South Fork Clearwater River - Newsome Creek RL-0039 TCGGAC  OtsNEWS10-0176 South Fork Clearwater River - Newsome Creek RL-0039 TTTAAT  OtsNEWS10-0179 South Fork Clearwater River - Newsome Creek RL-0039 ACCCCC  OtsNEWS10-0186 South Fork Clearwater River - Newsome Creek RL-0039 ATGTTG  OtsNEWS10-0188 South Fork Clearwater River - Newsome Creek RL-0039 CCTAAC  OtsNEWS10-0189 South Fork Clearwater River - Newsome Creek RL-0039 CTTTGC  OtsNEWS10-0194 South Fork Clearwater River - Newsome Creek RL-0039 GCTCAA  OtsNEWS10-0196 South Fork Clearwater River - Newsome Creek RL-0039 GTTGGA  OtsNEWS10-0207 South Fork Clearwater River - Newsome Creek RL-0039 TCTCGG  OtsNEWS10-2261 South Fork Clearwater River - Newsome Creek RL-0039 TTTGTC  OtsNEWS04-0753 South Fork Clearwater River - Newsome Creek RL-0039 CCGAGG  OtsNEWS04-0755 South Fork Clearwater River - Newsome Creek RL-0039 CTGGTT  OtsNEWS04-0758 South Fork Clearwater River - Newsome Creek RL-0039 GCGACC  OtsNEWS04-0759 South Fork Clearwater River - Newsome Creek RL-0039 GTGCCT  OtsNEWS08-0007 South Fork Clearwater River - Newsome Creek RL-0039 AATGAG  OtsNEWS08-0010 South Fork Clearwater River - Newsome Creek RL-0039 AGTAGG  OtsNEWS08-0011 South Fork Clearwater River - Newsome Creek RL-0039 CCATTT  OtsNEWS08-0013 South Fork Clearwater River - Newsome Creek RL-0039 CTACAG  OtsNEWS08-0014 South Fork Clearwater River - Newsome Creek RL-0039 GCAGAT  OtsNEWS08-0015 South Fork Clearwater River - Newsome Creek RL-0039 GTAAGT  OtsNEWS08-0020 South Fork Clearwater River - Newsome Creek RL-0039 TCAAAG  OtsNEWS08-0021 South Fork Clearwater River - Newsome Creek RL-0039 TTATGA  OtsNEWS08-0023 South Fork Clearwater River - Newsome Creek RL-0039 ACAAGA  OtsNEWS08-0025 South Fork Clearwater River - Newsome Creek RL-0039 AGTTAA  OtsNEWS08-0031 South Fork Clearwater River - Newsome Creek RL-0039 CCCGGT  OtsNEWS08-0032 South Fork Clearwater River - Newsome Creek RL-0039 CTCGCC  OtsNEWS08-0051 South Fork Clearwater River - Newsome Creek RL-0039 GCATGG  OtsNEWS08-0055 South Fork Clearwater River - Newsome Creek RL-0039 GTATCC  OtsNEWS08-0073 South Fork Clearwater River - Newsome Creek RL-0039 TCCTGC  OtsNEWS08-0080 South Fork Clearwater River - Newsome Creek RL-0039 TTCCGT  OtsNEWS08-0082 South Fork Clearwater River - Newsome Creek RL-0039 ACAGCG  OtsNEWS08-0083 South Fork Clearwater River - Newsome Creek RL-0039 ATAGTA  OtsNEWS08-0089 South Fork Clearwater River - Newsome Creek RL-0039 CCCTAA  OtsNEWS08-0411 South Fork Clearwater River - Newsome Creek RL-0039 CTGCGA  OtsNEWS08-0415 South Fork Clearwater River - Newsome Creek RL-0039 GCCGTA  OtsNEWS08-0519 South Fork Clearwater River - Newsome Creek RL-0039 GTCATC  OtsNEWS08-0598 South Fork Clearwater River - Newsome Creek RL-0039 TCGATT  OtsNEWS08-0601 South Fork Clearwater River - Newsome Creek RL-0039 TTCTAG  OtsNEWS08-0603 South Fork Clearwater River - Newsome Creek RL-0039 ACATAC  OtsNEWS08-0604 South Fork Clearwater River - Newsome Creek RL-0039 ATCAAA