title: DNA-Seq of Human Subgingival Microbiome: Former Tobacco Cigarette Smoker and current ENDS user
design: Subgingival plaque samples were collected and pooled from 15 sites on six maxillary and mandibular anterior teeth using sterile endodontic paper-points. Bacterial DNA was isolated from paper points, using the Qiagen DNA MiniAmp kit and quantified using a Qubit 3.0 fluorometer. Library generation was completed using an Illumina TruSeq kit. Quantified and pooled libraries were clustered on the Illumina HiSeq 4000 for 150bp PE sequencing.