title: GSM3168493: H3K27ac ChIP-seq EB SB; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde at 37 °C for 10 min and quenched with 0.125 M glycine for 5 min at room temperature. Then cells were scraped and ChIP was performed and DNA were extracted using a ChIP assay kit (Millipore) as described. For library construction and sequencing, ChIP-Seq DNA samples were quantified and quality assessed by Qubit 2.0 and Agilent 2100. DNA fragments range from 200-600bp were selected constructed for ChIP-Seq library with TruSeq ChIP Sample Prep Kit (Illumina) according to manufacturer's instructions. After scraped from plate, embryonic stem cells were washed with PBS and incubated in lysis buffer.
attributes.tag: GEO Accession
attributes.value: GSM3168493