title: RAD-Seq in O. tshawytscha from Morice River, British Columbia
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10NP0001 Morice River - British Columbia RL-0055 AATGAG  OtsCORE-10NP0002 Morice River - British Columbia RL-0055 AGTAGG  OtsCORE-10NP0003 Morice River - British Columbia RL-0055 CCATTT  OtsCORE-10NP0004 Morice River - British Columbia RL-0055 CTACAG  OtsCORE-10NP0005 Morice River - British Columbia RL-0055 GCAGAT  OtsCORE-10NP0006 Morice River - British Columbia RL-0055 GTAAGT  OtsCORE-10NP0007 Morice River - British Columbia RL-0055 TCAAAG  OtsCORE-10NP0008 Morice River - British Columbia RL-0055 TTATGA  OtsCORE-10NP0009 Morice River - British Columbia RL-0055 ACAAGA  OtsCORE-10NP0010 Morice River - British Columbia RL-0055 AGTTAA  OtsCORE-10NP0011 Morice River - British Columbia RL-0055 CCCGGT  OtsCORE-10NP0012 Morice River - British Columbia RL-0055 CTCGCC  OtsCORE-10NP0013 Morice River - British Columbia RL-0055 GCATGG  OtsCORE-10NP0014 Morice River - British Columbia RL-0055 GTATCC  OtsCORE-10NP0015 Morice River - British Columbia RL-0055 TCCTGC  OtsCORE-10NP0016 Morice River - British Columbia RL-0055 TTCCGT  OtsCORE-10NP0024 Morice River - British Columbia RL-0055 ACAGCG  OtsCORE-10NP0025 Morice River - British Columbia RL-0055 ATAGTA  OtsCORE-10NP0026 Morice River - British Columbia RL-0055 CCCTAA  OtsCORE-10NP0028 Morice River - British Columbia RL-0055 CTGCGA  OtsCORE-10NP0029 Morice River - British Columbia RL-0055 GCCGTA  OtsCORE-10NP0030 Morice River - British Columbia RL-0055 GTCATC  OtsCORE-10NP0031 Morice River - British Columbia RL-0055 TCGATT  OtsCORE-10NP0032 Morice River - British Columbia RL-0055 TTCTAG  OtsCORE-10NP0033 Morice River - British Columbia RL-0055 ACATAC  OtsCORE-10NP0034 Morice River - British Columbia RL-0055 ATCAAA  OtsCORE-10NP0035 Morice River - British Columbia RL-0055 CCGAGG  OtsCORE-10NP0044 Morice River - British Columbia RL-0055 CTGGTT  OtsCORE-10NP0045 Morice River - British Columbia RL-0055 GCGACC  OtsCORE-10NP0046 Morice River - British Columbia RL-0055 GTGCCT  OtsCORE-10NP0047 Morice River - British Columbia RL-0055 TCGCCA  OtsCORE-10NP0048 Morice River - British Columbia RL-0055 TTGAGC  OtsCORE-10NP0049 Morice River - British Columbia RL-0055 ACCATG  OtsCORE-10NP0057 Morice River - British Columbia RL-0055 ATGCAC  OtsCORE-10NP0058 Morice River - British Columbia RL-0055 CCGCAT  OtsCORE-10NP0059 Morice River - British Columbia RL-0055 CTTATG  OtsCORE-10NP0060 Morice River - British Columbia RL-0055 GCGCTG  OtsCORE-10NP0061 Morice River - British Columbia RL-0055 GTGTAA  OtsCORE-10NP0062 Morice River - British Columbia RL-0055 TCGGAC  OtsCORE-10NP0063 Morice River - British Columbia RL-0055 TTTAAT  OtsCORE-10NP0064 Morice River - British Columbia RL-0055 ACCCCC  OtsCORE-10NP0065 Morice River - British Columbia RL-0055 ATGTTG  OtsCORE-10NP0066 Morice River - British Columbia RL-0055 CCTAAC  OtsCORE-10NP0067 Morice River - British Columbia RL-0055 CTTTGC  OtsCORE-10NP0068 Morice River - British Columbia RL-0055 GCTCAA  OtsCORE-10NP0069 Morice River - British Columbia RL-0055 GTTGGA  OtsCORE-10NP0070 Morice River - British Columbia RL-0055 TCTCGG  OtsCORE-10NP0071 Morice River - British Columbia RL-0055 GAACCA