title: GSM3753526: YB-1 RIP-Seq wt Total (IP_YB1_H), run 2; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were lysed in two pellet volumes of lysis buffer and centrifuged at 16,000 g for 10 min. YB-1-RNA complex was isolated by antibody-coated Protein-G sepharose. RNA was isolated using TRIzol LS Reagent. Library was prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina. rRNA depletion was performed using NEBNext rRNA Depletion Kit (Human/Mouse/Rat).