title: RIP-Seq of m6A RNA in isolated mitochondria from Brassica oleracea inflorescence rep.2
design: Preparation of mitochondria from cauliflower inflorescence followed the protocol described in detail in (Neuwirt et al., 2005).RNA was prepared following standard TRI Reagent protocols (Molecular Research Center Inc., TR-118) and was treated with RNase-free DNase (Ambion, Cat no. AM2222). m6A mthylated RNA was immunoprecipitated using Monoclonal anti-m6A antibody (Synaptic systems, Cat no. 202011) bound to Protein A/G Magnetic Beads (Pierce, 88802). Purified m6A-RNA was sequenced on an Illumina Genome Analyzer.