title: GSM4677666: C3_4sU30min_Rep3; Mus musculus; OTHER
library_construction_protocol: Total RNAs were extracted according to TRIzol manuals, followed by DNaseI treatment.For each μg of total RNA, 2μL of Biotin-HPDP (Pierce, 50mg EZ-Link Biotin-HPDP), previously dissolved in DMF at a concentration of 1mg/mL, and 1μL of 10xBiotinylation buffer (100mM Tris HCl pH 7.4, 10mM EDTA), were added. The reaction was incubated with rotation for 15 min at 25ºC. Biotinylated 4sU-RNA was recovered using the μMacs Streptavidin Kit. Illumina TruSeq Stranded Total RNA Library Prep Kit
attributes.tag: GEO Accession
attributes.value: GSM4677666