title: 16S profiling of sample A03, Islet I, replicate "a"
design: Detailed description of sample preparation and data handling for 16S profiling is provided in Liu, et al., 2013 (mBio 4, e00076). Briefly, barcoded V3-V6 amplicons were generated using broad-coverage fusion PCR primers on 10 μl of DNA extracts in 50 μl PCR reactions. The barcoded amplicons were pooled and sequenced. Resulting pyrosequences were chimera-checked, and demultiplexed.