title: GSM3168255: in vitro-activated, LKB1-deficient CD8+ T cells rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extacted using Trizol following manufacturer's protocol. For cDNA synthesis, custom oligo-dT primers were used with a barcode and adapter-linker sequence (CCTACACGACGCTCTTCCGATCT—XXXXXXXX-T15). After first strand synthesis, samples were pooled together based on Actb qPCR values, and RNA-DNA hybrids degraded using consecutive acid-alkali treatment. A second sequencing linker (AGATCGGAAGAGCACACGTCTG) was ligated using T4 ligase (NEB) followed by SPRI clean-up. The mixture was then PCR enriched for 12 cycles and SPRI purified to yield final strand specific RNA-seq libraries as previously described (Jha et al., 2015) 3' RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM3168255