title: GSM1303507: hom_RNF17 _round_spermatids_smallRNA; Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA prepared with Trizol reagent (Invitrogen) was size selected (19-33nt) on 15% vertical polyacrylamide/urea gel. small RNA libraries were prepared according (Malone et al. Cold Spring Harb Protoc. 2012). Size selected RNA was ligated with 3' adaptor Modban oligonucleotide with mutant T4 RNA ligase 2, truncated (home made). The ligated products were sized fractionated on 15% vertical polyacrylamide/urea gel and ligated with 5' adaptor with T4 RNA ligase (Ambion). Then ligated products were separated on 15% vertical polyacrylamide/urea gel and used for reverse transcription with random hexamers and Superscript lll reverse transcriptase (Invitrogen). cDNA was amplified using Illumina primers and used for following sequencing on Illumina platforms.
attributes.tag: GEO Accession
attributes.value: GSM1303507