title: AL-1 cells DpnII
design: Culture cells were treated with 1% Trypsin at room temperature for 3 minutes, then Trypsin was quenched by addition of 10%FCS/AMEM. Cells were spun down and counted. 1x 106 cells were resuspended into 36ml of 10%FCS/AMEM. Restriction enzyme was DpnII