title: RNA-Seq of Danio rerio: 25 dpf gonad
design: RNA sequencing libraries were generated by extracting 3 g RNA per sample RNA from 25 dpf Danio rerio gonadal tissues. mRNA was then purified from the total RNA and fragmentation was carried out at elevated temperatures. Subsequent cDNA synthesis and size selection steps were carried out to generate fragments of 150-200 bp. PCR purification and quantification was carried out followed by clustering of the libraries. The libraries were then sequenced to generate 150 bp reads.