title: 16S rRNA  gene sequencing of poplar rhizosphere in greenhouse: genotype PB08 in  substrate Control
design: Libraries  for sequencing were prepared according to Illuminas 16S Metagenomic  Sequencing Library Preparation guide, with the exception of using Qiagen  HotStar Taq DNA polymerase for the first PCR (amplicon PCR). The template  specific primers were (without the overhang adapter sequence): 515F-Y  (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3). The first  PCR (amplicon PCR) was carried out for 35 cycles with annealing temperature  of 50C. Diluted pooled samples were loaded on an Illumina MiSeq and  sequenced using a 500-cycles MiSeq Reagent Kit v2.