title: WGS: soil: Reclamation_age_12
design: DNA extraction - NucleoSpin Soil extraction kit (Macherey-Nagel, Germany), Shearing - E220 Focused-ultrasonicator (Covaris, USA), target size 500 bp, Library preparation - the NEBNext Ultra II DNA Library Prep Kit for Illumina, Sequencing - MiSeq instrument (Illumina Inc., USA) using the Miseq Reagent Kit v3 for 600 cycles.