title: GSM1902353: muscle_D0; Sus scrofa; RNA-Seq
library_construction_protocol: Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA, USA).  Genomic DNA was removed by DNase I (Qiagen, Beijing, China). The quantity and quality of the RNA were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, California, USA). Ribosomal RNA was depleted using Ribo-Zero Magnetic kit (Epicentre, Madison, WI ). The mixed libraries were constructed by mixing equal quantities of each RNA sample. Strand-specific libraries for the paired-end sequencing were prepared using SMART or dUTP protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM1902353