title: Chaffinch Pect Pool
design: Pooled small RNA-seq libraries were prepared from RNA harvested from either foot lesions or pectoral muscle from animals CF180/09 and CF229/12. Equal volumes of total RNA were combined and ethanol precipitated. Recovered RNA was dissolved in nuclease free water (Ambion) and quantitated with a NanoDrop spectrophotometer (ThermoFisher). Libraries were prepared using 180 ng of pooled RNA with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300, New England Biolabs) according to the manufacturer s instructions with the addition of an additional round of indexing PCR to compensate for low input RNA. Libraries were quantitated with the Qubit dsDNA BR assay (ThermoFisher), QC checked with the Bioanalyzer High Sensitivity assay (Agilent Technologies), combined with other barcoded libraries, and sequenced on a single lane of a SR50 run on a HiSeq 4000 (Illumina) by the Genomic Sequencing and Analysis Facility at UT Austin.