title: LCM x PAXB F2s (pooled)
design: Genotyping-by-sequencing (GBS) reduced representation sequencing. Genomic DNA from 188 LCM x PAXB F2s were digested with ApeKI and ligated to one of 96 unique barcodes and sequenced with a total of 384 barcoded samples per lane and 4 index sequences. See supplemental file to Erickson et al 2016 (Evolution) for barcode and index information.