title: GSM3753472: 180221_KO_6DL_UNC1999_S9; Mus musculus; ATAC-seq
library_construction_protocol: Frozen ESC pellets were thawed and assessed for viability followed by nuclei preparation and treatment with Tn5 transposase. The samples were subjected to PCR amplification and DNA purification. Adapted from Buenostro et al., 2015; doi:10.1002/0471142727.mb2129s109 Libraries were generated and optimized by PCR amplification, purified, and verified for proper size selection using a Agilent BioAnalyzer To determine if the ATAC reactions were successful, an assessment of the overall enrichment of open chromatin regions was assayed by qPCR. Enrichment scores were calculated for each possible open vs closed region (2^(Ct_Open – Ct_Closed)). Scores over 10 were considered to be positively enriched.