title: Complete genomic sequence of Achromobacter spanius UQ283
design: 50 uL of glycerol stock was used to inoculate 15 mL of lysogeny broth (LB) and incubated overnight at 28C. Cells were harvested by centrifugation, removing the supernatant and resuspending it in 1.5 mL LB. DNA was then extracted from this sample using the QIAGEN Gentra Puregene Cell Kit as per the manufacturers instructions. The resultant DNA was submitted to the Ramacotti Institute (Sydney, Australia) for sequencing on a PacBio RSII machine, using one SMRT cell with P6-C4 chemistry and a 240-minute movie length. 20 kb SMRTbell libraries were constructed according to the manufacturers protocols, and size-selected (15 kb - 50 kb) using the BluePippin electrophoresis system (Sage Science, Massachusetts, USA).