title: RAD-Seq in O. tshawytscha from Pullen Creek Hatchery, AK
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-05PE0001 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 AATGAG  OtsCORE-05PE0002 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 AGTAGG  OtsCORE-05PE0003 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CCATTT  OtsCORE-05PE0006 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CTACAG  OtsCORE-05PE0007 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GCAGAT  OtsCORE-05PE0008 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GTAAGT  OtsCORE-05PE0009 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TCAAAG  OtsCORE-05PE0011 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TTATGA  OtsCORE-05PE0012 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ACAAGA  OtsCORE-05PE0013 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 AGTTAA  OtsCORE-05PE0014 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CCCGGT  OtsCORE-05PE0015 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CTCGCC  OtsCORE-05PE0016 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GCATGG  OtsCORE-05PE0017 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GTATCC  OtsCORE-05PE0018 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TCCTGC  OtsCORE-05PE0019 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TTCCGT  OtsCORE-05PE0020 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ACAGCG  OtsCORE-05PE0021 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ATAGTA  OtsCORE-05PE0022 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CCCTAA  OtsCORE-05PE0023 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CTGCGA  OtsCORE-05PE0024 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GCCGTA  OtsCORE-05PE0025 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GTCATC  OtsCORE-05PE0026 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TCGATT  OtsCORE-05PE0027 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TTCTAG  OtsCORE-05PE0028 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ACATAC  OtsCORE-05PE0029 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ATCAAA  OtsCORE-05PE0030 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CCGAGG  OtsCORE-05PE0031 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CTGGTT  OtsCORE-05PE0032 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GCGACC  OtsCORE-05PE0033 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GTGCCT  OtsCORE-05PE0034 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TCGCCA  OtsCORE-05PE0035 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TTGAGC  OtsCORE-05PE0036 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ACCATG  OtsCORE-05PE0037 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ATGCAC  OtsCORE-05PE0039 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CCGCAT  OtsCORE-05PE0041 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CTTATG  OtsCORE-05PE0042 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GCGCTG  OtsCORE-05PE0043 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GTGTAA  OtsCORE-05PE0044 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TCGGAC  OtsCORE-05PE0045 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TTTAAT  OtsCORE-05PE0046 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ACCCCC  OtsCORE-05PE0047 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 ATGTTG  OtsCORE-05PE0064 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CCTAAC  OtsCORE-05PE0069 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 CTTTGC  OtsCORE-05PE0071 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GCTCAA  OtsCORE-05PE0074 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 GTTGGA  OtsCORE-05PE0075 Pullen Creek Hatchery - Tahini R stock - Northern Southeast - AK RL-0052 TCTCGG