title: GSM1902535: siRRP40; Homo sapiens; OTHER
library_construction_protocol: Cells were washed once with Dulbecco’s phosphate-buffered saline and  collected in Trizol reagent (Ambion, Life Technologies) according to manufacturer’s  instructions, followed by treatment with DNase I (New England Biolabs) at 37°C for 30  min. RNA was extracted with phenol/chloroform equilibrated with 50 mM NaOAc (pH  5.0) and ethanol precipitated. Library preparation was modified from the protocol which published by Goldfard  and Cech (Goldfarb and Cech 2013). The samples were sequenced on the Illumina HiSeq using RapidSeq-100bp single-end  reads.
attributes.tag: GEO Accession
attributes.value: GSM1902535