title: GSM4677554: atac_col0_1; Arabidopsis thaliana; ATAC-seq
library_construction_protocol: Nuclei was extracted from 10-day-old whole seedlings. Next, 30,000 nuclei were counted and resuspended in 50 μL transposition reaction mix using the TruePrepTM DNA Library Prep Kit V2 for Illumina® (Vazyme, TD501). Reactions were incubated for 30 min at 37°C before isolation DNA using the VAHTSTM DNA Clean Beads (Vazyme, N411) Libraries were prepared by PCR amplification using two index (i5 and i7) Illumina barcodes (Vazyme, TD202) with 13 cycles. Libraries were purified with a VAHTSTM DNA Clean Beads.
attributes.tag: GEO Accession
attributes.value: GSM4677554