title: YKO deletion collection barcodes UP TAG
design: From 1 ug of RNA of each sample the TruSeq mRNA library was prepared following the manufacturer's instructions (Illumina). The quality of the libraries has been evaluated in BioRad Experion bioanalyzer and its concentration has been calculated by qPCR. The resulting libraries have been sequenced in a flow cell channel in a Illumina HiSeq2000, using a 100-cycle paired-end reads.