title: RNA-seq of infected leaf; midrib
design: Midrib-enriched leaf was ground in liquid N. Total RNA was extracted by Spectrum Total RNA kit (Sigma-Aldrich) from ca.100 mg of powder and DNase-treated.  RNA was evaluated with Bioanalyzer 2100 (Agilent Technologies). Library was prepared from 200 ng of total RNA with the TruSeq stranded Total RNA library Prep Plant Kit (Illumina).