title: Zea may of 500APPLUS Genotyping-by-sequencing
design: DNA concentrations are normalized to 10 ng/µl. ApeKI restriction enzyme is used to digest whole genome at 75 °C for two hours. Ligating the DNA fragments to adapters with different index sequence for each line using T4 DNA Ligase. After cleanup, a DNA panel including 96 lines is then pooled in one GBS library and PCR amplificationare performed. An additional procedure is processed for size selection using the MinElute Gel Extraction Kit,  which can improve the available sequencing data effectively. All the final libraries are measured the concentration again using qPCR to confirm the final concentration.