title: Comparison of traditional field methods, camera traps and blowfly-derived mammal DNA for tropical mammal diversity assessments
design: 205bp COI amplicon was generated using a two-step PCR that utilises universal mammal primers, Uni-Mini-bar F and RonPing R (Lee et al. 2015). Adaptor overhang sequences, TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG and GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG were added to the 5’ end of forward and reverse primers respectively. EconoTaq PLUS 2X Master Mix (Lucigen, USA) and COI Fast thermocycling program (Wilson 2012) were used to generate amplicons from 1 µL of DNA extract. 6 µL of product from the first round of PCR was used as the template for a second round of PCR. The libraries were quantified using qPCR.