title: GSM3754037: Control Rep3_miRNA; Rattus norvegicus; miRNA-Seq
library_construction_protocol: Total RNA was extracted with mirVana miRNA Isolation Kit (Cat #. AM1561, Austin TX, US) and its Standard Operating Protocol(SOP), which then experienced quality control with 2100 Bioanalyzer (Agilent Technologies Santa Clara, US). Library was constructed in steps: 3'-end  adapter ligation, 5'-end adapter ligation, reverse transcription, PCR amplification, size selection of cDNA library, and purification. These steps were completed with TruSeq Small RNA Sample Prep Kit (Illumina), T4 RNA Ligase 2 Truncated (BioLabs), SuperScript II Reverse Transcriptase (Invitrogen), 6% TBE Gel (1.0mm x 10well)(Invitrogen), Novex TBE Running Buffer (5X)(Invitrogen), Pellet Paint NF Co-precipitant (Novagen), Qubit dsDNA HS Assay Kit(Invitrogen), and Agilent High Sensitivity DNA Kit(Agilent).
attributes.tag: GEO Accession
attributes.value: GSM3754037