title: RNA-seq of late instar Drepana  arcuata
design: RNA was extracted from whole  bodies of late instars (Instar IV,V; N=5; flash frozen using liquid nitrogen)  using Norgen Biotek RNA extraction kit (Norgen Biotek Corp, Thorold, ON,  Canada). Larvae from single female were used in this study, for all the biological  replicates. Agilent Bioanalyser (Santa Clara, CA, United States) was used to  assess the quality and quantity of RNA samples. Library preparation and 150bp  paired end sequencing was carried out using TruSeq RNA v2 Library Prep Kit  and Illumina NextSeq500, respectively at the StemCore sequencing facility  (Ottawa Hospital Research Institute, ON, Canada).