title: Illumina HiSeq 2500 paired end sequencing; Multi-tissue transcriptome associated with feed efficiency in Nellore cattle
design: 100 bp paired-end RNAseq of poliA selected RNA on an Illumina HiSeq2500
library_construction_protocol: TruSeq Stranded mRNA LT Sample Prep
attributes.tag: assay type
attributes.value: RNA-seq of coding RNA
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 39
attributes.tag: experimental protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: extraction protocol
attributes.value: https://www.qiagen.com/se/resources/resourcedetail?id=14e7cf6e-521a-4cf7-8cbc-bf9f6fa33e24
attributes.tag: library preparation location
attributes.value: Centro de Genomica Funcional ESALQ-USP Piracicaba Brazil
attributes.tag: library preparation location longitude
attributes.value: -22.71
attributes.tag: library preparation location latitude
attributes.value: -47.63
attributes.tag: library preparation date
attributes.value: 2016-12-14
attributes.tag: sequencing location
attributes.value: Functional Genomic applied to Agriculture ESALQ-USP Piracicaba Brazil
attributes.tag: sequencing location longitude
attributes.value: -22.71
attributes.tag: sequencing location latitude
attributes.value: -47.63
attributes.tag: sequencing date
attributes.value: 2016-12-21
attributes.tag: experiment target
attributes.value: polyA RNA
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: library generation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
attributes.tag: read strand
attributes.value: mate 1 sense
attributes.tag: rna purity - 260:280 ratio
attributes.value: 2.1
attributes.tag: rna purity - 260:230 ratio
attributes.value: not collected
attributes.tag: rna integrity number
attributes.value: 8.2