title: mircobiology
design: MOBIO Power Soil DNA Isolation Kitwas used for extracting the genomic DNA of soil samples , the PCR amplification experiment went on a BioRad S1000 thermal cycler. The samples were evaluated using the electrophoresis on 1.0 % agarose and purified using the EZNA Gel Extraction Kit. The resulting microbial sequencing underwent on the Illumina Hiseq 2500 platform. Then the DNA library was built as recommended by the manufacturer for NEB Next Ultra DNA Library Prep Kit for Illumina.