title: Coniothyrium glycines PG-21 mitochondrion sequence
design: Total genomic DNA was prepared for sequencing using Nextera XT kit and run on Illumina MiSeq instrument to prepare 2X300 PE reads.  Reads were filtered on quality and trimmed to remove adapters. CLC Genomics Workbench was use to perform  de novo assembly, which yielded a  contig covering the entire mitochondrial replicon.  Contig was refined and closed using CLC Microbial Genome finishing module.  Reads were mapped back to finished  replicon, extracted, saved as fastq file and uploaded to SRA.