title: RNA-Seq of Camellia sinensis: nitrogen deficiency_replicate_2
design: Total RNA of each sample was isolated using a modified CTAB (Cetyltrimethylammonium bromide) method (Li et al., 2008) and then was purified using DNase I (Takara). Poly-A mRNA was enriched and then used to prepare paried-end cDNA library according to the manufacturer specifications (Illumina). Paried-end sequencing was performed using the Illumina Hiseq2000 platform.