title: 2nd biological replicate of B. glumae PG1 bgaI3 Mutant
design: Total RNA was extracted with the RNeasy Mini Kit (Qiagen) and chromosomal DNA was removed using DNase I (Fermentas). The Library was constructed with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) after rRNA depletion with Ribo-Zero for Bacteria (Epicentre).