title: RNA-seq of rainbow trout: adult female ovary-derived cell line
design: The 12 mRNA-Seq libraries were prepared using TruSeq  RNA Sample Preparation Kit (Illumina) according to the manufacturer's instructions. Briefly, Poly-A RNA were purified from 4 ?g of total RNA using oligo(dT) magnetic beads, fragmented and retro-transcribed using random primers. Complementary-DNAs were end-repaired and 3-adenylated, indexed adapters were then ligated. Ten rounds of PCR amplification were performed and the PCR products were size selected on a 2% agarose E-Gel (Thermo Scientific). Libraries were checked for quality on Agilent High Sensitivity DNA Kit and quantified with the QPCR NGS Library Quantification kit (Agilent Technologies). cDNA libraries were tagged and grouped in nine pools in equal ratios and sequenced in pair-end 2x100bp on Illumina HiSeq2000 with TruSeq  v3 Kit.