title: GSM1303468: hom RNF17 6 week old adult testis rep1 RNAseq; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was prepared with Trizol reagent (Invitrogen) Transcriptome libraries were prepared according  (Armour et al. 2009, Nat Methods) using not-so-random priming (NSR). 1 ug total RNA was revere transcribed using SuperScript III enzyme with first-strand NSR primer. RNA template was removed with RNase H (Invitrogen) Then cDNA was mixed with exo− Klenow fragment (NEB) second-strand NSR primer to synthesize the second strand. For PCR amplification, purified second-strand synthesis reaction were mixed with ExpandPLUS enzyme (Roche) and PE-P5-SBS3 and PE-P7-SBS8 primers. Products were run on a 2% low-melt agarose gel, and the 350–500 bp. range were purified.
attributes.tag: GEO Accession
attributes.value: GSM1303468