title: Original sequencing data
design: For Illumina sequencing, at least 1g genomic DNA was used for each strain in sequencing library construction. DNA samples were sheared into 400-500 bp fragments using a Covaris M220 Focused Acoustic Shearer following manufactures protocol. Illumina sequencing libraries were prepared from the sheared fragments using the NEXT flex Rapid DNA-Seq Kit. Briefly speaking, 5 prime ends were first end-repaired and phosphorylated. Next , the 3 ends were A-tailed and ligated to sequencing adapters. The third step is to enrich the adapters-ligated products using PCR. The prepared libraries then were used for paired-end Illumina sequencing (2  150 bp) on an Illumina HiSeq X Ten machine