title: Metataxonomics:bacteria:ethanol
design: Total DNA extraction was performed using the DNA extraction kit PowerSoil DNAisolation (Mobio / QIAGEN - USA), following the manufacturer&#39;s protocol. The V3-V4regions of interest in the extracted total DNA were amplified by an initial PCR reactionusing the primer forward (5&#39;TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG3&#39;) and reverse (5&#39;GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC 3&#39;), following the manufacturers protocol. The PCR products were purified toserve as templates for a second PCR reaction with the aim of preparing a library withthe Nextera XT Index Kit (Illumina  ). The quantification of libraries was performedusing the KAPA Library Quantification Kit (Kapa / Roche - USA).Paired-End (PE) DNA sequencing was performed on the Illumina  MiSeqplatform using the MiSeq  Reagent Kit v3 (600 cycle) and the PhiX Control Kit v3 kit(for quality control of DNA sequencing).