title: RAD-Seq in O. tshawytscha from Clearwater-Cherry Lane, ID & Warm Springs National Fish Hatchery, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCW08-8003 Clearwater-Cherry Lane RL-0046 AGGTGT  OtsCW08-8007 Clearwater-Cherry Lane RL-0046 TAATTC  OtsCW08-8010 Clearwater-Cherry Lane RL-0046 GGGCGC  OtsCW08-8016 Clearwater-Cherry Lane RL-0046 CGCGTG  OtsCW08-8017 Clearwater-Cherry Lane RL-0046 AACTGA  OtsCW08-8021 Clearwater-Cherry Lane RL-0046 TCTTCT  OtsCW08-8023 Clearwater-Cherry Lane RL-0046 TGACAA  OtsCW08-8024 Clearwater-Cherry Lane RL-0046 GATCGT  OtsCW08-8025 Clearwater-Cherry Lane RL-0046 CGCAGA  OtsCW08-8028 Clearwater-Cherry Lane RL-0046 GACTCT  OtsCW08-8030 Clearwater-Cherry Lane RL-0046 TGAACC  OtsCW08-8032 Clearwater-Cherry Lane RL-0046 AGCCAT  OtsCW08-8034 Clearwater-Cherry Lane RL-0046 GGTTTG  OtsCW08-8036 Clearwater-Cherry Lane RL-0046 AAGACG  OtsCW08-8039 Clearwater-Cherry Lane RL-0046 AAGCTA  OtsCW08-8041 Clearwater-Cherry Lane RL-0046 TGCTTA  OtsCW08-8043 Clearwater-Cherry Lane RL-0046 TACGGG  OtsCW08-8050 Clearwater-Cherry Lane RL-0046 CGTGAT  OtsCW08-8052 Clearwater-Cherry Lane RL-0046 TATCAC  OtsCW08-8055 Clearwater-Cherry Lane RL-0046 GACGAC  OtsCW08-8056 Clearwater-Cherry Lane RL-0046 AGGGTC  OtsCW08-8059 Clearwater-Cherry Lane RL-0046 ACTCTT  OtsCW08-8062 Clearwater-Cherry Lane RL-0046 AACGTT  OtsCW08-8063 Clearwater-Cherry Lane RL-0046 GGGGCG  OtsCW08-8070 Clearwater-Cherry Lane RL-0046 ACTGGC  OtsCW08-8071 Clearwater-Cherry Lane RL-0046 CACCTC  OtsCW08-8072 Clearwater-Cherry Lane RL-0046 CAGGCA  OtsCW08-8073 Clearwater-Cherry Lane RL-0046 GGCAAG  OtsCW08-8082 Clearwater-Cherry Lane RL-0046 AAACGG  OtsCW08-8087 Clearwater-Cherry Lane RL-0046 CGTCTA  OtsCW08-8089 Clearwater-Cherry Lane RL-0046 GAGAGA  OtsCW08-8090 Clearwater-Cherry Lane RL-0046 CATACT  OtsCW08-8091 Clearwater-Cherry Lane RL-0046 CGAGGC  OtsCW08-8095 Clearwater-Cherry Lane RL-0046 TGGGGA  OtsCW08-8096 Clearwater-Cherry Lane RL-0046 TGCCCG  OtsCW08-8098 Clearwater-Cherry Lane RL-0046 GAAATG  OtsCW08-8100 Clearwater-Cherry Lane RL-0046 GGACTT  OtsCW08-8101 Clearwater-Cherry Lane RL-0046 CGGTCC  OtsCW08-8104 Clearwater-Cherry Lane RL-0046 CAATCG  OtsCW08-8107 Clearwater-Cherry Lane RL-0046 GAACCA  OtsCW08-8109 Clearwater-Cherry Lane RL-0046 AATATC  OtsCW08-8110 Clearwater-Cherry Lane RL-0046 TAAGCT  OtsCW08-8111 Clearwater-Cherry Lane RL-0046 GGTACA  OtsCW08-8112 Clearwater-Cherry Lane RL-0046 CAAAAA  OtsCW08-8117 Clearwater-Cherry Lane RL-0046 TAGTAT  OtsCW08-8120 Clearwater-Cherry Lane RL-0046 TACACA  OtsCW08-8121 Clearwater-Cherry Lane RL-0046 AGCGCA  0-Ots-270-01 Warm Springs NFH RL-0046 GCCGTA  0-Ots-270-02 Warm Springs NFH RL-0046 ATCAAA  0-Ots-270-03 Warm Springs NFH RL-0046 GTTGGA  0-Ots-270-04 Warm Springs NFH RL-0046 TCGGAC  0-Ots-270-05 Warm Springs NFH RL-0046 GCGACC  0-Ots-270-06 Warm Springs NFH RL-0046 CTGGTT  0-Ots-270-07 Warm Springs NFH RL-0046 TTTAAT  0-Ots-270-08 Warm Springs NFH RL-0046 ACAGCG  0-Ots-270-09 Warm Springs NFH RL-0046 ATAGTA  0-Ots-270-10 Warm Springs NFH RL-0046 AATGAG  0-Ots-270-11 Warm Springs NFH RL-0046 GCTCAA  0-Ots-270-12 Warm Springs NFH RL-0046 GTATCC  0-Ots-270-13 Warm Springs NFH RL-0046 CTTTGC  0-Ots-270-14 Warm Springs NFH RL-0046 TCCTGC  0-Ots-270-15 Warm Springs NFH RL-0046 CTACAG  0-Ots-270-20 Warm Springs NFH RL-0046 ATGCAC  0-Ots-270-21 Warm Springs NFH RL-0046 GCAGAT  0-Ots-270-22 Warm Springs NFH RL-0046 GTGTAA  0-Ots-270-23 Warm Springs NFH RL-0046 CCTAAC  0-Ots-270-25 Warm Springs NFH RL-0046 CCCTAA  0-Ots-270-26 Warm Springs NFH RL-0046 TCTCGG  0-Ots-270-27 Warm Springs NFH RL-0046 TTTGTC  0-Ots-270-28 Warm Springs NFH RL-0046 AGTTAA  0-Ots-270-30 Warm Springs NFH RL-0046 ATGTTG  0-Ots-270-31 Warm Springs NFH RL-0046 ACAAGA  0-Ots-270-38 Warm Springs NFH RL-0046 GCGCTG  0-Ots-270-39 Warm Springs NFH RL-0046 AGTAGG  0-Ots-270-42 Warm Springs NFH RL-0046 CTGCGA  0-Ots-270-43 Warm Springs NFH RL-0046 GTAAGT  0-Ots-270-44 Warm Springs NFH RL-0046 CCATTT  0-Ots-270-47 Warm Springs NFH RL-0046 GTGCCT  0-Ots-270-48 Warm Springs NFH RL-0046 TTGAGC  0-Ots-270-49 Warm Springs NFH RL-0046 TTCCGT  0-Ots-270-50 Warm Springs NFH RL-0046 CCGCAT  0-Ots-270-52 Warm Springs NFH RL-0046 CTCGCC  0-Ots-270-54 Warm Springs NFH RL-0046 TCGATT  0-Ots-270-55 Warm Springs NFH RL-0046 CTTATG  0-Ots-270-57 Warm Springs NFH RL-0046 CCGAGG  0-Ots-270-58 Warm Springs NFH RL-0046 TTCTAG  0-Ots-270-62 Warm Springs NFH RL-0046 TCAAAG  0-Ots-270-63 Warm Springs NFH RL-0046 GTCATC  0-Ots-270-65 Warm Springs NFH RL-0046 TTATGA  0-Ots-270-66 Warm Springs NFH RL-0046 TCGCCA  0-Ots-270-67 Warm Springs NFH RL-0046 ACATAC  0-Ots-270-69 Warm Springs NFH RL-0046 GCATGG  0-Ots-270-70 Warm Springs NFH RL-0046 CCCGGT  0-Ots-270-71 Warm Springs NFH RL-0046 ACCATG  0-Ots-270-72 Warm Springs NFH RL-0046 ACCCCC