title: GSM2072715: IDH2.FLT3_ag221_3 RRBS; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA was extracted using the Allprep DNA/RNA purification Kit (Qiagen) and analyzed using a Bioanalyzer (Agilent Technologies). ERRBS libraries were prepared by the Weill Cornell Medicine Epigenomics Core according to a previously published protocol (Akalin et al., PLoSGenetics, 2012; Garrett-Bakelman, F.E. and Sheridan, C., JoVE, 2015). Briefly, high molecular weight DNA was disgested with MspI followed by end repair and adapter ligation. Size selection was perfomed for 150-400bp fragments ad bisulfite conversion was performed prior to PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM2072715