title: RAD-Seq in O. tshawytscha from Lyons Ferry Hatchery - Tucannon stock & Pahsimeroi Hatchery, ID
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsPBT10-2623-2904-1 Lyons Ferry FH - Tucannon stock RL-0089 AATGAG  OtsPBT10-2623-2904-10 Lyons Ferry FH - Tucannon stock RL-0089 TCAAAG  OtsPBT10-2623-2904-11 Lyons Ferry FH - Tucannon stock RL-0089 TTATGA  OtsPBT10-2623-2904-12 Lyons Ferry FH - Tucannon stock RL-0089 ACAAGA  OtsPBT10-2623-2904-13 Lyons Ferry FH - Tucannon stock RL-0089 AGTTAA  OtsPBT10-2623-2904-14 Lyons Ferry FH - Tucannon stock RL-0089 CCCGGT  OtsPBT10-2623-2904-15 Lyons Ferry FH - Tucannon stock RL-0089 CTCGCC  OtsPBT10-2623-2904-16 Lyons Ferry FH - Tucannon stock RL-0089 GCATGG  OtsPBT10-2623-2904-17 Lyons Ferry FH - Tucannon stock RL-0089 GTATCC  OtsPBT10-2623-2904-18 Lyons Ferry FH - Tucannon stock RL-0089 TCCTGC  OtsPBT10-2623-2904-19 Lyons Ferry FH - Tucannon stock RL-0089 TTCCGT  OtsPBT10-2623-2904-2 Lyons Ferry FH - Tucannon stock RL-0089 AGTAGG  OtsPBT10-2623-2904-20 Lyons Ferry FH - Tucannon stock RL-0089 ACAGCG  OtsPBT10-2623-2904-21 Lyons Ferry FH - Tucannon stock RL-0089 ATAGTA  OtsPBT10-2623-2904-22 Lyons Ferry FH - Tucannon stock RL-0089 CCCTAA  OtsPBT10-2623-2904-23 Lyons Ferry FH - Tucannon stock RL-0089 CTGCGA  OtsPBT10-2623-2904-24 Lyons Ferry FH - Tucannon stock RL-0089 GCCGTA  OtsPBT10-2623-2904-25 Lyons Ferry FH - Tucannon stock RL-0089 GTCATC  OtsPBT10-2623-2904-26 Lyons Ferry FH - Tucannon stock RL-0089 TCGATT  OtsPBT10-2623-2904-27 Lyons Ferry FH - Tucannon stock RL-0089 TTCTAG  OtsPBT10-2623-2904-29 Lyons Ferry FH - Tucannon stock RL-0089 ACATAC  OtsPBT10-2623-2904-3 Lyons Ferry FH - Tucannon stock RL-0089 CCATTT  OtsPBT10-2623-2904-32 Lyons Ferry FH - Tucannon stock RL-0089 ATCAAA  OtsPBT10-2623-2904-36 Lyons Ferry FH - Tucannon stock RL-0089 CCGAGG  OtsPBT10-2623-2904-38 Lyons Ferry FH - Tucannon stock RL-0089 CTGGTT  OtsPBT10-2623-2904-4 Lyons Ferry FH - Tucannon stock RL-0089 CTACAG  OtsPBT10-2623-2904-40 Lyons Ferry FH - Tucannon stock RL-0089 GCGACC  OtsPBT10-2623-2904-42 Lyons Ferry FH - Tucannon stock RL-0089 GTGCCT  OtsPBT10-2623-2904-45 Lyons Ferry FH - Tucannon stock RL-0089 TCGCCA  OtsPBT10-2623-2904-46 Lyons Ferry FH - Tucannon stock RL-0089 TTGAGC  OtsPBT10-2623-2904-47 Lyons Ferry FH - Tucannon stock RL-0089 ACCATG  OtsPBT10-2623-2904-48 Lyons Ferry FH - Tucannon stock RL-0089 ATGCAC  OtsPBT10-2623-2904-5 Lyons Ferry FH - Tucannon stock RL-0089 GCAGAT  OtsPBT10-2623-2904-50 Lyons Ferry FH - Tucannon stock RL-0089 CCGCAT  OtsPBT10-2623-2904-51 Lyons Ferry FH - Tucannon stock RL-0089 CTTATG  OtsPBT10-2623-2904-53 Lyons Ferry FH - Tucannon stock RL-0089 GCGCTG  OtsPBT10-2623-2904-56 Lyons Ferry FH - Tucannon stock RL-0089 GTGTAA  OtsPBT10-2623-2904-57 Lyons Ferry FH - Tucannon stock RL-0089 TCGGAC  OtsPBT10-2623-2904-58 Lyons Ferry FH - Tucannon stock RL-0089 TTTAAT  OtsPBT10-2623-2904-6 Lyons Ferry FH - Tucannon stock RL-0089 GTAAGT  OtsPBT10-2623-2904-60 Lyons Ferry FH - Tucannon stock RL-0089 ACCCCC  OtsPBT10-2623-2904-63 Lyons Ferry FH - Tucannon stock RL-0089 ATGTTG  OtsPBT10-2623-2904-64 Lyons Ferry FH - Tucannon stock RL-0089 CCTAAC  OtsPBT10-2623-2904-67 Lyons Ferry FH - Tucannon stock RL-0089 CTTTGC  OtsPBT10-2623-2904-69 Lyons Ferry FH - Tucannon stock RL-0089 GCTCAA  OtsPBT10-2623-2904-78 Lyons Ferry FH - Tucannon stock RL-0089 GTTGGA  OtsPBT10-2623-2904-79 Lyons Ferry FH - Tucannon stock RL-0089 TCTCGG  OtsPBT10-2623-2904-81 Lyons Ferry FH - Tucannon stock RL-0089 TTTGTC  OtsPAHS10S_0001 Pahsimeroi RL-0089 AAACGG  OtsPAHS10S_0002 Pahsimeroi RL-0089 ACTCTT  OtsPAHS10S_0003 Pahsimeroi RL-0089 ATTCCG  OtsPAHS10S_0005 Pahsimeroi RL-0089 CGAGGC  OtsPAHS10S_0006 Pahsimeroi RL-0089 GAAATG  OtsPAHS10S_0007 Pahsimeroi RL-0089 GGACTT  OtsPAHS10S_0008 Pahsimeroi RL-0089 TAAGCT  OtsPAHS10S_0009 Pahsimeroi RL-0089 TCTTCT  OtsPAHS10S_0011 Pahsimeroi RL-0089 AACGTT  OtsPAHS10S_0012 Pahsimeroi RL-0089 ACTGGC  OtsPAHS10S_0013 Pahsimeroi RL-0089 CAAAAA  OtsPAHS10S_0014 Pahsimeroi RL-0089 CGCAGA  OtsPAHS10S_0015 Pahsimeroi RL-0089 GAACCA  OtsPAHS10S_0016 Pahsimeroi RL-0089 GGCAAG  OtsPAHS10S_0018 Pahsimeroi RL-0089 TGAACC  OtsPAHS10S_0019 Pahsimeroi RL-0089 AACTGA  OtsPAHS10S_0020 Pahsimeroi RL-0089 AGCCAT  OtsPAHS10S_0023 Pahsimeroi RL-0089 CAATCG  OtsPAHS10S_0024 Pahsimeroi RL-0089 CGCGTG  OtsPAHS10S_0025 Pahsimeroi RL-0089 GACGAC  OtsPAHS10S_0026 Pahsimeroi RL-0089 GGGCGC  OtsPAHS10S_0027 Pahsimeroi RL-0089 TACACA  OtsPAHS10S_0028 Pahsimeroi RL-0089 TGACAA  OtsPAHS10S_0029 Pahsimeroi RL-0089 AAGACG  OtsPAHS10S_0030 Pahsimeroi RL-0089 AGCGCA  OtsPAHS10S_0031 Pahsimeroi RL-0089 CACCTC  OtsPAHS10S_0032 Pahsimeroi RL-0089 CGGTCC  OtsPAHS10S_0034 Pahsimeroi RL-0089 GACTCT  OtsPAHS10S_0037 Pahsimeroi RL-0089 GGGGCG  OtsPAHS10S_0039 Pahsimeroi RL-0089 TACGGG  OtsPAHS10S_0040 Pahsimeroi RL-0089 TGCCCG  OtsPAHS10S_0041 Pahsimeroi RL-0089 AAGCTA  OtsPAHS10S_0044 Pahsimeroi RL-0089 AGGGTC  OtsPAHS10S_0051 Pahsimeroi RL-0089 CAGGCA  OtsPAHS10S_0052 Pahsimeroi RL-0089 CGTCTA  OtsPAHS10S_0054 Pahsimeroi RL-0089 GAGAGA  OtsPAHS10S_0058 Pahsimeroi RL-0089 GGTACA  OtsPAHS10S_0061 Pahsimeroi RL-0089 TAGTAT  OtsPAHS10S_0062 Pahsimeroi RL-0089 TGCTTA  OtsPAHS10S_0065 Pahsimeroi RL-0089 AATATC  OtsPAHS10S_0066 Pahsimeroi RL-0089 AGGTGT  OtsPAHS10S_0067 Pahsimeroi RL-0089 CATACT  OtsPAHS10S_0070 Pahsimeroi RL-0089 CGTGAT  OtsPAHS10S_0076 Pahsimeroi RL-0089 GATCGT  OtsPAHS10S_0077 Pahsimeroi RL-0089 GGTTTG  OtsPAHS10S_0080 Pahsimeroi RL-0089 TATCAC  OtsPAHS10S_0081 Pahsimeroi RL-0089 TGGGGA