title: GSM4676914: E11-A; Mus musculus; RNA-Seq
library_construction_protocol: From each female, one embryo or pup was decapitated and the cerebral cortex was collected for RNA sequencing. A biological sample is a cortex isolated form one embryo (or pup). Three biological samples from 3 different litters were collected per age. The cortices of embryos or pups were immediately lysed for RNA extraction using the Nucleospin® RNA Kit (Macherey-Nagel) according to the manufacturer's protocol. Messenger (polyA+) RNAs were purified from 1 µg of total RNA using oligo(dT) Libraries were prepared using the strand specific RNA-Seq library preparation TruSeq Stranded mRNA kit (Illumina). Libraries were multiplexed by 12 on a flowcell. A 75 bp single read sequencing was performed on a NextSeq 500 device (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4676914