title: Illumina HiSeq 2500 paired end sequencing; Dual RNA-seq of peripheral blood from Gambian children with severe or uncomplicated Plasmodium falciparum malaria
design: Dual RNA-seq of peripheral blood from Gambian children with severe or uncomplicated Plasmodium falciparum malaria
library_construction_protocol: Venous blood was collected into PAXgene blood RNA tube (BD). Total RNA was extracted using PAXgene blood RNA kit (Qiagen). Libraries were prepared with 1μg of total RNA using the ScriptSeq v2 RNA-seq library preparation kit (Illumina). Globin-Zero Gold kit (Epicentre) was used to remove ribosomal RNA and globin messenger RNA.
attributes.tag: Experimental Factor: disease
attributes.value: cerebral malaria and hyperlactatemia