title: RAD-Seq in O. tshawytscha from Sawtooth Hatchery, ID & Lostine River
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsLR09-090699 Lostine River weir RL-0042 CTACAG  OtsLR09-091046 Lostine River weir RL-0042 GTATCC  OtsLR09-091131 Lostine River weir RL-0042 TTCCGT  OtsLR09-091184 Lostine River weir RL-0042 ACAGCG  OtsLR09-091206 Lostine River weir RL-0042 ATAGTA  OtsLR09-091239 Lostine River weir RL-0042 CCCTAA  OtsLR09-091387 Lostine River weir RL-0042 CTGCGA  OtsLR09-091422 Lostine River weir RL-0042 GCCGTA  OtsLR09-091600 Lostine River weir RL-0042 GTCATC  OtsLR09-091645 Lostine River weir RL-0042 TCGATT  OtsLR09-091749 Lostine River weir RL-0042 TTCTAG  OtsLR09-09185 Lostine River weir RL-0042 AATGAG  OtsLR09-092195 Lostine River weir RL-0042 ACATAC  OtsLR09-092201 Lostine River weir RL-0042 ATCAAA  OtsLR09-092203 Lostine River weir RL-0042 CCGAGG  OtsLR09-092210 Lostine River weir RL-0042 CTGGTT  OtsLR09-092225 Lostine River weir RL-0042 GCGACC  OtsLR09-092227 Lostine River weir RL-0042 GTGCCT  OtsLR09-092237 Lostine River weir RL-0042 TCGCCA  OtsLR09-092245 Lostine River weir RL-0042 TTGAGC  OtsLR09-092247 Lostine River weir RL-0042 ACCATG  OtsLR09-092250 Lostine River weir RL-0042 ATGCAC  OtsLR09-092257 Lostine River weir RL-0042 CCGCAT  OtsLR09-092275 Lostine River weir RL-0042 CTTATG  OtsLR09-092277 Lostine River weir RL-0042 GCGCTG  OtsLR09-092281 Lostine River weir RL-0042 GTGTAA  OtsLR09-092290 Lostine River weir RL-0042 TCGGAC  OtsLR09-092310 Lostine River weir RL-0042 TTTAAT  OtsLR09-092326 Lostine River weir RL-0042 ATGTTG  OtsLR09-092327 Lostine River weir RL-0042 CCTAAC  OtsLR09-092332 Lostine River weir RL-0042 CTTTGC  OtsLR09-092340 Lostine River weir RL-0042 GCTCAA  OtsLR09-092341 Lostine River weir RL-0042 GTTGGA  OtsLR09-092343 Lostine River weir RL-0042 TCTCGG  OtsLR09-092352 Lostine River weir RL-0042 TTTGTC  OtsLR09-09487 Lostine River weir RL-0042 AGTAGG  OtsLR09-09511 Lostine River weir RL-0042 CCATTT  OtsLR09-09566 Lostine River weir RL-0042 GTAAGT  OtsLR09-09577 Lostine River weir RL-0042 TCAAAG  OtsLR09-09606 Lostine River weir RL-0042 TTATGA  OtsLR09-09620 Lostine River weir RL-0042 ACAAGA  OtsLR09-09725 Lostine River weir RL-0042 GCAGAT  OtsLR09-09853 Lostine River weir RL-0042 AGTTAA  OtsLR09-09857 Lostine River weir RL-0042 CCCGGT  OtsLR09-09860 Lostine River weir RL-0042 CTCGCC  OtsLR09-09922 Lostine River weir RL-0042 GCATGG  OtsLR09-09973 Lostine River weir RL-0042 TCCTGC  OtsSAWT11S_0201 Sawtooth Hatchery RL-0042 AAACGG  OtsSAWT11S_0204 Sawtooth Hatchery RL-0042 ACTCTT  OtsSAWT11S_0205 Sawtooth Hatchery RL-0042 ATTCCG  OtsSAWT11S_0208 Sawtooth Hatchery RL-0042 CGAGGC  OtsSAWT11S_0211 Sawtooth Hatchery RL-0042 GAAATG  OtsSAWT11S_0212 Sawtooth Hatchery RL-0042 GGACTT  OtsSAWT11S_0213 Sawtooth Hatchery RL-0042 TAAGCT  OtsSAWT11S_0217 Sawtooth Hatchery RL-0042 TCTTCT  OtsSAWT11S_0218 Sawtooth Hatchery RL-0042 AACGTT  OtsSAWT11S_0219 Sawtooth Hatchery RL-0042 ACTGGC  OtsSAWT11S_0221 Sawtooth Hatchery RL-0042 CAAAAA  OtsSAWT11S_0223 Sawtooth Hatchery RL-0042 CGCAGA  OtsSAWT11S_0224 Sawtooth Hatchery RL-0042 GAACCA  OtsSAWT11S_0225 Sawtooth Hatchery RL-0042 GGCAAG  OtsSAWT11S_0226 Sawtooth Hatchery RL-0042 TAATTC  OtsSAWT11S_0227 Sawtooth Hatchery RL-0042 TGAACC  OtsSAWT11S_0228 Sawtooth Hatchery RL-0042 AACTGA  OtsSAWT11S_0229 Sawtooth Hatchery RL-0042 AGCCAT  OtsSAWT11S_0231 Sawtooth Hatchery RL-0042 CAATCG  OtsSAWT11S_0233 Sawtooth Hatchery RL-0042 CGCGTG  OtsSAWT11S_0234 Sawtooth Hatchery RL-0042 GACGAC  OtsSAWT11S_0235 Sawtooth Hatchery RL-0042 GGGCGC  OtsSAWT11S_0236 Sawtooth Hatchery RL-0042 TACACA  OtsSAWT11S_0237 Sawtooth Hatchery RL-0042 TGACAA  OtsSAWT11S_0238 Sawtooth Hatchery RL-0042 AAGACG  OtsSAWT11S_0239 Sawtooth Hatchery RL-0042 AGCGCA  OtsSAWT11S_0240 Sawtooth Hatchery RL-0042 CACCTC  OtsSAWT11S_0242 Sawtooth Hatchery RL-0042 CGGTCC  OtsSAWT11S_0243 Sawtooth Hatchery RL-0042 GACTCT  OtsSAWT11S_0245 Sawtooth Hatchery RL-0042 GGGGCG  OtsSAWT11S_0247 Sawtooth Hatchery RL-0042 TACGGG  OtsSAWT11S_0248 Sawtooth Hatchery RL-0042 TGCCCG  OtsSAWT11S_0249 Sawtooth Hatchery RL-0042 AAGCTA  OtsSAWT11S_0250 Sawtooth Hatchery RL-0042 AGGGTC  OtsSAWT11S_0251 Sawtooth Hatchery RL-0042 CAGGCA  OtsSAWT11S_0252 Sawtooth Hatchery RL-0042 CGTCTA  OtsSAWT11S_0253 Sawtooth Hatchery RL-0042 GAGAGA  OtsSAWT11S_0254 Sawtooth Hatchery RL-0042 GGTACA  OtsSAWT11S_0255 Sawtooth Hatchery RL-0042 TAGTAT  OtsSAWT11S_0256 Sawtooth Hatchery RL-0042 TGCTTA  OtsSAWT11S_0260 Sawtooth Hatchery RL-0042 AATATC  OtsSAWT11S_0261 Sawtooth Hatchery RL-0042 AGGTGT  OtsSAWT11S_0262 Sawtooth Hatchery RL-0042 CATACT  OtsSAWT11S_0263 Sawtooth Hatchery RL-0042 CGTGAT  OtsSAWT11S_0264 Sawtooth Hatchery RL-0042 GATCGT  OtsSAWT11S_0265 Sawtooth Hatchery RL-0042 GGTTTG  OtsSAWT11S_0268 Sawtooth Hatchery RL-0042 TATCAC  OtsSAWT11S_0269 Sawtooth Hatchery RL-0042 TGGGGA