title: RAD-Seq in O. tshawytscha from Wells Hatchery, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-08HY0001 Wells Hatchery RL-0058 AATGAG  OtsCORE-08HY0002 Wells Hatchery RL-0058 AGTAGG  OtsCORE-08HY0003 Wells Hatchery RL-0058 CCATTT  OtsCORE-08HY0006 Wells Hatchery RL-0058 CTACAG  OtsCORE-08HY0007 Wells Hatchery RL-0058 GCAGAT  OtsCORE-08HY0008 Wells Hatchery RL-0058 GTAAGT  OtsCORE-08HY0009 Wells Hatchery RL-0058 TCAAAG  OtsCORE-08HY0010 Wells Hatchery RL-0058 TTATGA  OtsCORE-08HY0011 Wells Hatchery RL-0058 ACAAGA  OtsCORE-08HY0017 Wells Hatchery RL-0058 AGTTAA  OtsCORE-08HY0018 Wells Hatchery RL-0058 CCCGGT  OtsCORE-08HY0019 Wells Hatchery RL-0058 CTCGCC  OtsCORE-08HY0021 Wells Hatchery RL-0058 GCATGG  OtsCORE-08HY0022 Wells Hatchery RL-0058 GTATCC  OtsCORE-08HY0024 Wells Hatchery RL-0058 TCCTGC  OtsCORE-08HY0026 Wells Hatchery RL-0058 TTCCGT  OtsCORE-08HY0028 Wells Hatchery RL-0058 ACAGCG  OtsCORE-08HY0031 Wells Hatchery RL-0058 ATAGTA  OtsCORE-08HY0032 Wells Hatchery RL-0058 CCCTAA  OtsCORE-08HY0034 Wells Hatchery RL-0058 CTGCGA  OtsCORE-08HY0035 Wells Hatchery RL-0058 GCCGTA  OtsCORE-08HY0036 Wells Hatchery RL-0058 GTCATC  OtsCORE-08HY0037 Wells Hatchery RL-0058 TCGATT  OtsCORE-08HY0038 Wells Hatchery RL-0058 TTCTAG  OtsCORE-08HY0039 Wells Hatchery RL-0058 ACATAC  OtsCORE-08HY0040 Wells Hatchery RL-0058 ATCAAA  OtsCORE-08HY0041 Wells Hatchery RL-0058 CCGAGG  OtsCORE-08HY0056 Wells Hatchery RL-0058 CTGGTT  OtsCORE-08HY0058 Wells Hatchery RL-0058 GCGACC  OtsCORE-08HY0079 Wells Hatchery RL-0058 GTGCCT  OtsCORE-08HY0086 Wells Hatchery RL-0058 TCGCCA  OtsCORE-08HY0087 Wells Hatchery RL-0058 TTGAGC  OtsCORE-08HY0088 Wells Hatchery RL-0058 ACCATG  OtsCORE-08HY0089 Wells Hatchery RL-0058 ATGCAC  OtsCORE-08HY0090 Wells Hatchery RL-0058 CCGCAT  OtsCORE-08HY0095 Wells Hatchery RL-0058 CTTATG  OtsCORE-08HY0096 Wells Hatchery RL-0058 GCGCTG  OtsCORE-08HY0097 Wells Hatchery RL-0058 GTGTAA  OtsCORE-08HY0098 Wells Hatchery RL-0058 TCGGAC  OtsCORE-08HY0099 Wells Hatchery RL-0058 TTTAAT  OtsCORE-08HY0100 Wells Hatchery RL-0058 ACCCCC  OtsCORE-08HY0101 Wells Hatchery RL-0058 ATGTTG  OtsCORE-08HY0102 Wells Hatchery RL-0058 CCTAAC  OtsCORE-08HY0103 Wells Hatchery RL-0058 CTTTGC  OtsCORE-08HY0127 Wells Hatchery RL-0058 GCTCAA  OtsCORE-08HY0128 Wells Hatchery RL-0058 GTTGGA  OtsCORE-08HY0129 Wells Hatchery RL-0058 TCTCGG  OtsCORE-08HY0130 Wells Hatchery RL-0058 TTTGTC