title: RAD-Seq in O. tshawytscha from Priest Rapids Hatchery, WA & Cowlitz Hatchery, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  OtsCORE-10JO0001 Cowlitz Salmon Hatchery RL-0033 AATGAG  OtsCORE-10JO0002 Cowlitz Salmon Hatchery RL-0033 AGTAGG  OtsCORE-10JO0004 Cowlitz Salmon Hatchery RL-0033 CCATTT  OtsCORE-10JO0005 Cowlitz Salmon Hatchery RL-0033 CTACAG  OtsCORE-10JO0008 Cowlitz Salmon Hatchery RL-0033 GCAGAT  OtsCORE-10JO0012 Cowlitz Salmon Hatchery RL-0033 GTAAGT  OtsCORE-10JO0013 Cowlitz Salmon Hatchery RL-0033 TCAAAG  OtsCORE-10JO0014 Cowlitz Salmon Hatchery RL-0033 TTATGA  OtsCORE-10JO0018 Cowlitz Salmon Hatchery RL-0033 ACAAGA  OtsCORE-10JO0019 Cowlitz Salmon Hatchery RL-0033 AGTTAA  OtsCORE-10JO0024 Cowlitz Salmon Hatchery RL-0033 CCCGGT  OtsCORE-10JO0029 Cowlitz Salmon Hatchery RL-0033 CTCGCC  OtsCORE-10JO0030 Cowlitz Salmon Hatchery RL-0033 GCATGG  OtsCORE-10JO0039 Cowlitz Salmon Hatchery RL-0033 GTATCC  OtsCORE-10JO0043 Cowlitz Salmon Hatchery RL-0033 TCCTGC  OtsCORE-10JO0044 Cowlitz Salmon Hatchery RL-0033 TTCCGT  OtsCORE-10JO0045 Cowlitz Salmon Hatchery RL-0033 ACAGCG  OtsCORE-10JO0046 Cowlitz Salmon Hatchery RL-0033 ATAGTA  OtsCORE-10JO0047 Cowlitz Salmon Hatchery RL-0033 CCCTAA  OtsCORE-10JO0048 Cowlitz Salmon Hatchery RL-0033 CTGCGA  OtsCORE-10JO0050 Cowlitz Salmon Hatchery RL-0033 GCCGTA  OtsCORE-10JO0051 Cowlitz Salmon Hatchery RL-0033 GTCATC  OtsCORE-10JO0052 Cowlitz Salmon Hatchery RL-0033 TCGATT  OtsCORE-10JO0053 Cowlitz Salmon Hatchery RL-0033 TTCTAG  OtsCORE-10JO0054 Cowlitz Salmon Hatchery RL-0033 ACATAC  OtsCORE-10JO0055 Cowlitz Salmon Hatchery RL-0033 ATCAAA  OtsCORE-10JO0056 Cowlitz Salmon Hatchery RL-0033 CCGAGG  OtsCORE-10JO0059 Cowlitz Salmon Hatchery RL-0033 CTGGTT  OtsCORE-10JO0060 Cowlitz Salmon Hatchery RL-0033 GCGACC  OtsCORE-10JO0063 Cowlitz Salmon Hatchery RL-0033 GTGCCT  OtsCORE-10JO0064 Cowlitz Salmon Hatchery RL-0033 TCGCCA  OtsCORE-10JO0065 Cowlitz Salmon Hatchery RL-0033 TTGAGC  OtsCORE-10JO0066 Cowlitz Salmon Hatchery RL-0033 ACCATG  OtsCORE-10JO0067 Cowlitz Salmon Hatchery RL-0033 ATGCAC  OtsCORE-10JO0068 Cowlitz Salmon Hatchery RL-0033 CCGCAT  OtsCORE-10JO0069 Cowlitz Salmon Hatchery RL-0033 CTTATG  OtsCORE-10JO0071 Cowlitz Salmon Hatchery RL-0033 GCGCTG  OtsCORE-10JO0073 Cowlitz Salmon Hatchery RL-0033 GTGTAA  OtsCORE-10JO0077 Cowlitz Salmon Hatchery RL-0033 TCGGAC  OtsCORE-10JO0078 Cowlitz Salmon Hatchery RL-0033 TTTAAT  OtsCORE-10JO0081 Cowlitz Salmon Hatchery RL-0033 ACCCCC  OtsCORE-10JO0082 Cowlitz Salmon Hatchery RL-0033 ATGTTG  OtsCORE-10JO0086 Cowlitz Salmon Hatchery RL-0033 CCTAAC  OtsCORE-10JO0087 Cowlitz Salmon Hatchery RL-0033 CTTTGC  OtsCORE-10JO0088 Cowlitz Salmon Hatchery RL-0033 GCTCAA  OtsCORE-10JO0093 Cowlitz Salmon Hatchery RL-0033 GTTGGA  OtsCORE-10JO0096 Cowlitz Salmon Hatchery RL-0033 TCTCGG  OtsCORE-10JO0099 Cowlitz Salmon Hatchery RL-0033 TTTGTC  OtsCORE-10IP0019 Priest Rapids Hatchery RL-0033 AAACGG  OtsCORE-10IP0020 Priest Rapids Hatchery RL-0033 ACTCTT  OtsCORE-10IP0022 Priest Rapids Hatchery RL-0033 ATTCCG  OtsCORE-10IP0033 Priest Rapids Hatchery RL-0033 CGAGGC  OtsCORE-10IP0034 Priest Rapids Hatchery RL-0033 GAAATG  OtsCORE-10IP0035 Priest Rapids Hatchery RL-0033 GGACTT  OtsCORE-10IP0036 Priest Rapids Hatchery RL-0033 TAAGCT  OtsCORE-10IP0037 Priest Rapids Hatchery RL-0033 TCTTCT  OtsCORE-10IP0038 Priest Rapids Hatchery RL-0033 AACGTT  OtsCORE-10IP0041 Priest Rapids Hatchery RL-0033 ACTGGC  OtsCORE-10IP0042 Priest Rapids Hatchery RL-0033 CAAAAA  OtsCORE-10IP0043 Priest Rapids Hatchery RL-0033 CGCAGA  OtsCORE-10IP0045 Priest Rapids Hatchery RL-0033 GAACCA  OtsCORE-10IP0047 Priest Rapids Hatchery RL-0033 GGCAAG  OtsCORE-10IP0048 Priest Rapids Hatchery RL-0033 TAATTC  OtsCORE-10IP0049 Priest Rapids Hatchery RL-0033 TGAACC  OtsCORE-10IP0050 Priest Rapids Hatchery RL-0033 AACTGA  OtsCORE-10IP0051 Priest Rapids Hatchery RL-0033 AGCCAT  OtsCORE-10IP0054 Priest Rapids Hatchery RL-0033 CAATCG  OtsCORE-10IP0055 Priest Rapids Hatchery RL-0033 CGCGTG  OtsCORE-10IP0056 Priest Rapids Hatchery RL-0033 GACGAC  OtsCORE-10IP0057 Priest Rapids Hatchery RL-0033 GGGCGC  OtsCORE-10IP0059 Priest Rapids Hatchery RL-0033 TACACA  OtsCORE-10IP0060 Priest Rapids Hatchery RL-0033 TGACAA  OtsCORE-10IP0061 Priest Rapids Hatchery RL-0033 AAGACG  OtsCORE-10IP0064 Priest Rapids Hatchery RL-0033 AGCGCA  OtsCORE-10IP0065 Priest Rapids Hatchery RL-0033 CACCTC  OtsCORE-10IP0066 Priest Rapids Hatchery RL-0033 CGGTCC  OtsCORE-10IP0067 Priest Rapids Hatchery RL-0033 GACTCT  OtsCORE-10IP0068 Priest Rapids Hatchery RL-0033 GGGGCG  OtsCORE-10IP0071 Priest Rapids Hatchery RL-0033 TGCCCG  OtsCORE-10IP0072 Priest Rapids Hatchery RL-0033 AAGCTA  OtsCORE-10IP0073 Priest Rapids Hatchery RL-0033 AGGGTC  OtsCORE-10IP0075 Priest Rapids Hatchery RL-0033 CGTCTA  OtsCORE-10IP0076 Priest Rapids Hatchery RL-0033 GAGAGA  OtsCORE-10IP0077 Priest Rapids Hatchery RL-0033 GGTACA  OtsCORE-10IP0079 Priest Rapids Hatchery RL-0033 TAGTAT  OtsCORE-10IP0080 Priest Rapids Hatchery RL-0033 TGCTTA  OtsCORE-10IP0081 Priest Rapids Hatchery RL-0033 AATATC  OtsCORE-10IP0082 Priest Rapids Hatchery RL-0033 AGGTGT  OtsCORE-10IP0084 Priest Rapids Hatchery RL-0033 CATACT  OtsCORE-10IP0085 Priest Rapids Hatchery RL-0033 CGTGAT  OtsCORE-10IP0086 Priest Rapids Hatchery RL-0033 GATCGT  OtsCORE-10IP0087 Priest Rapids Hatchery RL-0033 GGTTTG  OtsCORE-10IP0088 Priest Rapids Hatchery RL-0033 TATCAC  OtsCORE-10IP0090 Priest Rapids Hatchery RL-0033 TGGGGA