title: GSM2417214: MEF_PE_mRNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: oligodt-selected mRNA RNA was treated on column with 0.5 kunitz units of DNase prior to elution according to manufacturers instructions. RNA from MEF cultures induced for 48h to express OSKM/Esrrb, OSKM/Fra1, OSKM/cJun or a single reprogramming factor (tetO-Oct4, tetO-Sox2, or tetO–Klf4, tetO–Myc) was also isolated. Messenger RNA was captured using oligodT Dynabeads (Life Technologies). Strand-specific RNA-seq libraries were constructed using a TruSeq Stranded mRNA library Prep Kit, following the manufacturers instructions (RS-122-2101). Stranded mRNA Library
attributes.tag: GEO Accession
attributes.value: GSM2417214