title: RNAseq of Y.lipolytica W29 on glycerol replicate 1
design: Continuous cultivations in chemostats have been used and biomass samples were taken at steady state growth. Bead-milling (TissueLyser, Quiagen) was used for cell disruption and homogenization. RNA extraction by RNeasy Plus Mini Kit (Quiagen). Library prep with TruSeq total RNA kit (Illumina). Samples were preped seperetly with diffrent barcodes and pooled before sequencing (multiplexing). After sequencing procedure, demultiplexing was performed by Barcode Splitter tool from the FASTX toolkit version 0.0.14 (Hannon lab) allowing 1 mismatch.