title: vaginal mucosal 16S rRNA survey- Sample: p6z1tr19
design: DNA was extracted using the Bacteremia Kit (Mobio). The V3-V4 region of the 16S rRNA gene was targeted for broad-range PCR with pyrosequencing. 6-bp barcodes were used with the reverse primer to facilitate multiplexing. Reactions were purified using Agencourt AMPure beads prior to sequencing.