title: GSM2335666: mut_unt_hmeDIPSeq; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was sonicated to approximately 200–400 bp with a Covaris E-220 Sonicator (Covaris) and end-repaired using standard Illumina library preparation protocol. DNA was immunoprecipitated with anti-5-hydroxymethylcytidine antibody. Immunoabsorbed DNA fragments were recovered by adding a rabbit anti-mouse IgG secondary antibody (2.5 mg/ml, Jackson ImmunoResearch) and incubating for 2h. To complete library preparation, the immuno-precipitated DNA was purified and subjected to two 10-cycle PCR reactions using 16 μl of DNA for each sample with paired-end Illumina PCR primers. A final size selection was performed to select all fragments <700 bp in size by electrophoresis in 8% polyacrylamide gel.  Genomic DNA/RNA was extracted using the Qiagen AllPrep Mini Kit according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2335666