title: GSM4565566: HepG2-nop53-mRNA-seq rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the cultured cells using Trizol Reagent (Invitrogen) according to the manufacturer's instructions.mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, and double stranded cDNA synthesized using random hexamers  cDNA was synthesized using random hexamers, M-MuLV Reverse Transcriptase (RNase H), and DNA Polymerase I.The library fragments are purified with AMPure XP system RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4565566