title: DNA-seq of Aeromicrobium sp. S592
design: The genomic DNA of  sample was extracted by SDS .Then the purity and integrity of the DNA were detected by agarose gel electrophoresis, and quantified was examined by Qubit. The DNA samples qualified by electrophoresis were randomly broken into fragments with a length of about 350 bp by Covaris ultrasonic breaker. After processing the DNA fragments, use the NEBNext Ultra  DNA Library Prep Kit for Illumina (NEB, USA) kit to complete the entire library preparation through the steps of end repair, adding A tail, adding sequencing adapter, purification, PCR amplification and other steps.After the library construction is completed, first use Qubit 2.0 for preliminary quantification, dilute the library to 2ng / ul, and then use Agilent 2100 to detect the inserts of the library. After the insert size is as expected, use Q-PCR to accurately determine the effective concentration of the library Quantify to ensure library quality.