title: small intestine contents of male Wistar rats
design: Diet restriction experiment: 12 male rats (4 weeks old) were fed ad libitum for 3 weeks and then 6 (selected randomly) were fed ad libitum(HC group) and the other 6 were fed 60% of the ad libitum(HCR group) for 5 weeks.  At week 12, their intestinal content samples were collected .small intestine content's sequencing method:16sRNA sequencing technique.