title: Shotgun sequencing of total RNA from the brain of reptarenavirus infected ball python L
design: The goal of this study was to test the hypothesis that reptarenavirus infection causes inclusion body disease. This dataset was generated by shotgun sequencing total RNA from a tissue sample from a single infected (or control uninfected) snake. Briefly, total RNA was extracted from a frozen tissue sample and converted into dsDNA. Adapters were added to library molecules using the Nextera reagent (Illumina) and PCR. Libraries were multiplexed and sequenced on an Illumina MiSeq instrument. Please see associated publication for more detailed descriptions of library preparation and analysis protocols.