title: MiSeq of DNA from Microbes: Jackson Springs 2
design: Microbes were concentrated from spring water via TFF. The concentrates were then filtered through a 0.22 m Sterivex filter (Millipore) to capture the microbial fraction and these filters were cut out of the sterivex and inserted into the Mo Bio PowerSoil_ DNA Isolation Kit. Microbial DNA was extracted following the manufacturers protocol. The extracted bacterial DNA underwent amplicon library preparation and Illumina sequencing of the 16S-V4 rRNA hypervariable region using 16S-V4 515f and 806r primers on a MiSeq system.