title: Metatranscriptome profiling in the sea adjacent to Chuuk, Federated States of Micronesiaon on September, 2014
design: Total RNA was extracted using the QIAeasy Plus Viral DNA/RNA Extraction Kit (Intron, Seongnam, Korea) according to the manufacturer’s instructions. DNA was digested by DNaseI treatment. The RNA library for pyrosequencing was prepared using the TruSeq Nano RNA library preparation kit according to the manufacturer’s manual (Illumina, San Diego, USA). The prepared RNA library was paired-end-sequenced using Illumina HiSeq 2000, which was performed by Macrogen, Seoul, Korea.