title: GSM3753324: Puro_3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolated using Qiagen RNeasy Plus Mini kit (gDNA elimination column used) from MCF10A human mammary epithelial cells mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA was first fragmented randomly by addition of fragmentation buffer, then NEB library was prepared. Q-PCR is used to accurately quantify the library effective concentration (> 2nM), in order to ensure the library quality. Libraries fed into Illumina Platform, and paired-end reads were generated
attributes.tag: GEO Accession
attributes.value: GSM3753324