title: GSM2071869: 48h_2mg_R3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated with the mirVana PARIS Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. Stranded mRNA libraries for Illumina sequencing were prepared using the NEBNext(r) Ultra Directional RNA kits from New England Biolabs, Inc. according to the manufacturer's protocol (Version 2.0 July 2013).  Actinomycin D was added during cDNA synthesis to ensure high levels of strand specificity.  All libraries were run on a Bionanalyzer to confirm library size and yield.  Barcoded libraries were normalized and pooled based on concentrations determined by qPCR with Library Quantification kits from KAPA Biosystems.  
attributes.tag: GEO Accession
attributes.value: GSM2071869