title: GSM3753534: RNA-Seq wt Total rep1 (total_H_1), run 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in two pellet volumes of lysis buffer and centrifuged at 16,000 g for 10 min. RNA was isolated using TRIzol LS Reagent. Library was prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina. rRNA depletion was performed using NEBNext rRNA Depletion Kit (Human/Mouse/Rat).