title: RNA-Seq of Homo Sapien neurologically healthy control: Postmortem Cervical Spinal Section Tissue: Sample BennettLab_neurologically_healthy_control_16
attributes.tag: library_construction_and_experimental_design_description
attributes.value: "20 20 micron sections of cervical spinal tissue embedded in OCT were cut using a Cryostat and placed in Qiazol provided in Qiagen miRNeasy's kit. We homogenized the tissue in Qiazol with a thin syringe and needle, and we followed the rest of the Qiagen miRNeasy kit's instructions with the optional DNase treatment. Libraries were then prepared using 500 ng RNA input into Illumina's Stranded Total RNA kit after ensuring the RNA was not significantly degraded and had an RQI score >7 with the Biorad Experion. "