title: 16S amplicon-seq of viral hepatitis  UKKMD025
design: DNA from fecal samples was isolated using  the RNeasy Power Microbiome Kit (Qiagen, Germany). Seven of the 9 variable  bacterial 16S rDNA regions (pool 1: V2, V4 and V8; pool 2: V3, V6/7 and V9)  were amplified with the Ion 16S Metagenomics Kit (Thermo Fisher Scienctific,  USA) utilizing two primer pools. Amplicons were pooled and cleaned using the  NucleoMag NGS Clean-up (Macherey-Nagel, Germany), followed by library  preparation with the Ion Plus Fragment Library Kit (Thermo Fisher  Scienctific, USA).