title: RAD-Seq in O. tshawytscha from Klamath River, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsKlam04-001 Klamath RL-0063 AATGAG  OtsKlam04-004 Klamath RL-0063 AGTAGG  OtsKlam04-007 Klamath RL-0063 CCATTT  OtsKlam04-008 Klamath RL-0063 CTACAG  OtsKlam04-010 Klamath RL-0063 GCAGAT  OtsKlam04-012 Klamath RL-0063 GTAAGT  OtsKlam04-015 Klamath RL-0063 TCAAAG  OtsKlam04-022 Klamath RL-0063 TTATGA  OtsKlam04-060 Klamath RL-0063 ACAAGA  OtsKlam04-064 Klamath RL-0063 AGTTAA  OtsKlam04-065 Klamath RL-0063 CCCGGT  OtsKlam04-068 Klamath RL-0063 CTCGCC  OtsKlam04-071 Klamath RL-0063 GCATGG  OtsKlam04-073 Klamath RL-0063 GTATCC  OtsKlam04-074 Klamath RL-0063 TCCTGC  OtsKlam04-078 Klamath RL-0063 TTCCGT  OtsKlam04-079 Klamath RL-0063 ACAGCG  OtsKlam04-080 Klamath RL-0063 ATAGTA  OtsKlam04-081 Klamath RL-0063 CCCTAA  OtsKlam04-084 Klamath RL-0063 CTGCGA  OtsKlam04-085 Klamath RL-0063 GCCGTA  OtsKlam04-086 Klamath RL-0063 GTCATC  OtsKlam04-087 Klamath RL-0063 TCGATT  OtsKlam04-088 Klamath RL-0063 TTCTAG  OtsKlam04-090 Klamath RL-0063 ACATAC  OtsKlam04-092 Klamath RL-0063 ATCAAA  OtsKlam04-093 Klamath RL-0063 CCGAGG  OtsKlam04-094 Klamath RL-0063 CTGGTT  OtsKlam04-096 Klamath RL-0063 GCGACC  OtsKlam04-097 Klamath RL-0063 GTGCCT  OtsKlam04-099 Klamath RL-0063 TCGCCA  OtsKlam04-100 Klamath RL-0063 TTGAGC  OtsKlam04-103 Klamath RL-0063 ATGCAC  OtsKlam04-106 Klamath RL-0063 CCGCAT  OtsKlam04-107 Klamath RL-0063 CTTATG  OtsKlam04-111 Klamath RL-0063 GCGCTG  OtsKlam04-114 Klamath RL-0063 GTGTAA  OtsKlam04-115 Klamath RL-0063 TCGGAC  OtsKlam04-116 Klamath RL-0063 TTTAAT  OtsKlam04-117 Klamath RL-0063 ACCCCC  OtsKlam04-118 Klamath RL-0063 ATGTTG  OtsKlam04-119 Klamath RL-0063 CCTAAC  OtsKlam04-120 Klamath RL-0063 CTTTGC  OtsKlam04-124 Klamath RL-0063 GCTCAA  OtsKlam04-127 Klamath RL-0063 GTTGGA  OtsKlam04-128 Klamath RL-0063 TCTCGG  OtsKlam04-129 Klamath RL-0063 TTTGTC  OtsKlam04-130 Klamath RL-0063 ACCATG