title: RAD-Seq in O. tshawytscha from George River Weir, AK
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-05PG0003 George River Weir - AK RL-0056 AATGAG  OtsCORE-05PG0004 George River Weir - AK RL-0056 AGTAGG  OtsCORE-05PG0007 George River Weir - AK RL-0056 CCATTT  OtsCORE-05PG0010 George River Weir - AK RL-0056 CTACAG  OtsCORE-05PG0011 George River Weir - AK RL-0056 GCAGAT  OtsCORE-05PG0012 George River Weir - AK RL-0056 GTAAGT  OtsCORE-05PG0013 George River Weir - AK RL-0056 TCAAAG  OtsCORE-05PG0015 George River Weir - AK RL-0056 TTATGA  OtsCORE-05PG0016 George River Weir - AK RL-0056 ACAAGA  OtsCORE-05PG0021 George River Weir - AK RL-0056 AGTTAA  OtsCORE-05PG0022 George River Weir - AK RL-0056 CCCGGT  OtsCORE-05PG0025 George River Weir - AK RL-0056 CTCGCC  OtsCORE-05PG0029 George River Weir - AK RL-0056 GCATGG  OtsCORE-05PG0030 George River Weir - AK RL-0056 GTATCC  OtsCORE-05PG0031 George River Weir - AK RL-0056 TCCTGC  OtsCORE-05PG0032 George River Weir - AK RL-0056 TTCCGT  OtsCORE-05PG0033 George River Weir - AK RL-0056 ACAGCG  OtsCORE-05PG0035 George River Weir - AK RL-0056 ATAGTA  OtsCORE-05PG0037 George River Weir - AK RL-0056 CCCTAA  OtsCORE-05PG0039 George River Weir - AK RL-0056 CTGCGA  OtsCORE-05PG0040 George River Weir - AK RL-0056 GCCGTA  OtsCORE-05PG0042 George River Weir - AK RL-0056 GTCATC  OtsCORE-05PG0043 George River Weir - AK RL-0056 TCGATT  OtsCORE-05PG0045 George River Weir - AK RL-0056 TTCTAG  OtsCORE-05PG0046 George River Weir - AK RL-0056 ACATAC  OtsCORE-05PG0048 George River Weir - AK RL-0056 ATCAAA  OtsCORE-05PG0049 George River Weir - AK RL-0056 CCGAGG  OtsCORE-05PG0051 George River Weir - AK RL-0056 CTGGTT  OtsCORE-05PG0058 George River Weir - AK RL-0056 GCGACC  OtsCORE-05PG0061 George River Weir - AK RL-0056 GTGCCT  OtsCORE-05PG0062 George River Weir - AK RL-0056 TCGCCA  OtsCORE-05PG0064 George River Weir - AK RL-0056 TTGAGC  OtsCORE-05PG0066 George River Weir - AK RL-0056 ACCATG  OtsCORE-05PG0068 George River Weir - AK RL-0056 ATGCAC  OtsCORE-05PG0070 George River Weir - AK RL-0056 CCGCAT  OtsCORE-05PG0071 George River Weir - AK RL-0056 CTTATG  OtsCORE-05PG0073 George River Weir - AK RL-0056 GCGCTG  OtsCORE-05PG0075 George River Weir - AK RL-0056 GTGTAA  OtsCORE-05PG0077 George River Weir - AK RL-0056 TCGGAC  OtsCORE-05PG0078 George River Weir - AK RL-0056 TTTAAT  OtsCORE-05PG0082 George River Weir - AK RL-0056 ACCCCC  OtsCORE-05PG0084 George River Weir - AK RL-0056 ATGTTG  OtsCORE-05PG0085 George River Weir - AK RL-0056 CCTAAC  OtsCORE-05PG0091 George River Weir - AK RL-0056 CTTTGC  OtsCORE-05PG0097 George River Weir - AK RL-0056 GCTCAA  OtsCORE-05PG0098 George River Weir - AK RL-0056 GTTGGA  OtsCORE-05PG0099 George River Weir - AK RL-0056 TCTCGG  OtsCORE-05PG0100 George River Weir - AK RL-0056 CGTCTA