title: AMPLICON of soil metagenome: 16S rRNA  gene Carter Creek old-field
design: DNA extraction using MoBio PowerMag Microbiome RNA/DNA Isolation Kit. Amplified V4 region of 16S rRNA gene using dual indexed primers with Kozich et al. 2013 scheme. Primers targeted 515f and 806r. Triplicate reactions were pooled, normalized with SequalPrep normaliztion plate kit, and 400 bp DNA was selected using gel extraction. Pooled libraries run on Illumuna MiSeq with 2x250 V2 kit. Raw reads were demultiplexed using custom script.