title: GSM3555711: PR18; Mus musculus; RNA-Seq
library_construction_protocol: To extract total RNA in mouse brain, 1 volume of brain homogenate was mixed with 3 volumes of Trizol LS Reagent (10296010, Thermo Fisher Scientific), and frozen on dry ice. One day later, total RNA was extracted using the Direct-zolTM RNA MiniPrep kit (R2073, Zymo Research) according to the manufacturer's instruction. A total of 200 ng of RNA in a 50 μl volume was used for preparation of paired-end sequencing libraries using the TruSeq Stranded Total Sample Preparation kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3555711