title: GSM2413596: Mouse_ThioMac_RNA_GW-3_EDM_15-07-16; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using Trizol and Direct-zol columns (Zymo) PolyA mRNA was selected from total RNA prior to fragmentation, first strand and second strand synthesis incorporating deoxy-UTP. Following dA-tailing and barcode adapter ligation the product was PCR amplified and subsequently size-selected (223-375 bp) using TBE gel electrophoresis.  The excised library was purified prior to sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2413596