title: GSM3753580: R24_mushroombody; Apis mellifera; RNA-Seq
library_construction_protocol: Brains were fresh-dissected in ice-cold Dulbecco's phosphate-buffered saline (DPBS), and the MBs were dissected in one sample. Both samples were then cut into small pieces and incubated for 2 min in an Eppendorf tube containing 90 μl of DPBS and 10 μl of 0.25% Trypsin with 2.21 mM EDTA. Next, 90 μl of the solution was removed and replaced with DPBS, and brain tissue was gently triturated with a pipette tip to dissociated cells. Fixation and rehydration were carried out in accordance with 10x protocols, with a 15 min methanol fixation time on ice and storage at -20°C for less than one week before rehydration and sequencing. scRNA-Seq libraries were constructed with the Chromium Single Cell 3' Library and Gel Bead Kit v2 and were sequenced on a 2 x 150nt lane in a HiSeq 4000 (Illumina, San Diego, CA).
attributes.tag: GEO Accession
attributes.value: GSM3753580