title: GSM1631681: Small RNAs in WT C. elegans under L4440 RNAi; Caenorhabditis elegans; miRNA-Seq
library_construction_protocol: Total RNA isolation was carried out using TriReagent (Ambion) following the vendor’s protocol with the following modifications: Samples in TriReagent were subjected to three rounds of freeze-thaw-vortex cycles to improve RNA extraction efficiency. RNAs were precipitated in isopropanol for 1 h at -80°C followed by three washes with 70% ethanol. Total RNA was submitted to BGI for small RNA-seq library construction and deep sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1631681