title: RAD-Seq in O. tshawytscha from Chamberlain Creek, ID and Tucannon River, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCham10-0003 Chamberlain Cr RL-0065 AAACGG  OtsCham10-0008 Chamberlain Cr RL-0065 ACTCTT  OtsCham10-0009 Chamberlain Cr RL-0065 ATTCCG  OtsCham10-0010 Chamberlain Cr RL-0065 CGAGGC  OtsCham10-0012 Chamberlain Cr RL-0065 GAAATG  OtsCham10-0023 Chamberlain Cr RL-0065 GGACTT  OtsCham10-0030 Chamberlain Cr RL-0065 TAAGCT  OtsCham10-0037 Chamberlain Cr RL-0065 TCTTCT  OtsCham10-0040 Chamberlain Cr RL-0065 AACGTT  OtsCham10-0049 Chamberlain Cr RL-0065 ACTGGC  OtsCham11-0001 Chamberlain Cr RL-0065 CAAAAA  OtsCham11-0002 Chamberlain Cr RL-0065 CGCAGA  OtsCham11-0003 Chamberlain Cr RL-0065 GAACCA  OtsCham11-0004 Chamberlain Cr RL-0065 GGCAAG  OtsCham11-0005 Chamberlain Cr RL-0065 TAATTC  OtsCham11-0006 Chamberlain Cr RL-0065 TGAACC  OtsCham11-0007 Chamberlain Cr RL-0065 AACTGA  OtsCham11-0008 Chamberlain Cr RL-0065 AGCCAT  OtsCham11-0009 Chamberlain Cr RL-0065 CAATCG  OtsCham11-0010 Chamberlain Cr RL-0065 CGCGTG  OtsCham11-0011 Chamberlain Cr RL-0065 GACGAC  OtsCham11-0012 Chamberlain Cr RL-0065 GGGCGC  OtsCham11-0013 Chamberlain Cr RL-0065 TACACA  OtsCham11-0014 Chamberlain Cr RL-0065 TGACAA  OtsCham11-0015 Chamberlain Cr RL-0065 AAGACG  OtsCham11-0016 Chamberlain Cr RL-0065 AGCGCA  OtsCham11-0017 Chamberlain Cr RL-0065 CACCTC  OtsCham11-0019 Chamberlain Cr RL-0065 CGGTCC  OtsCham11-0021 Chamberlain Cr RL-0065 GACTCT  OtsCham11-0022 Chamberlain Cr RL-0065 GGGGCG  OtsCham11-0023 Chamberlain Cr RL-0065 TACGGG  OtsCham11-0024 Chamberlain Cr RL-0065 TGCCCG  OtsCham11-0025 Chamberlain Cr RL-0065 AAGCTA  OtsCham11-0026 Chamberlain Cr RL-0065 AGGGTC  OtsCham11-0030 Chamberlain Cr RL-0065 CAGGCA  OtsCham11-0031 Chamberlain Cr RL-0065 CGTCTA  OtsCham11-0032 Chamberlain Cr RL-0065 GAGAGA  OtsCham11-0033 Chamberlain Cr RL-0065 GGTACA  OtsCham11-0035 Chamberlain Cr RL-0065 TAGTAT  OtsCham11-0036 Chamberlain Cr RL-0065 TGCTTA  OtsCham11-0038 Chamberlain Cr RL-0065 AATATC  OtsCham11-0039 Chamberlain Cr RL-0065 AGGTGT  OtsCham11-0040 Chamberlain Cr RL-0065 CATACT  OtsCham11-0041 Chamberlain Cr RL-0065 CGTGAT  OtsCham11-0043 Chamberlain Cr RL-0065 GATCGT  OtsCham11-0047 Chamberlain Cr RL-0065 GGTTTG  OtsCham11-0048 Chamberlain Cr RL-0065 TATCAC  OtsCham11-0049 Chamberlain Cr RL-0065 TGGGGA  03EK-10 Tucannon RL-0065 CTACAG  03EK-11 Tucannon RL-0065 GCAGAT  03EK-13 Tucannon RL-0065 GTAAGT  03EK-14 Tucannon RL-0065 TCAAAG  03EK-18 Tucannon RL-0065 ACAAGA  03EK-19 Tucannon RL-0065 AGTTAA  03EK-22 Tucannon RL-0065 CCCGGT  03EK-23 Tucannon RL-0065 CTCGCC  03EK-26 Tucannon RL-0065 GCATGG  03EK-27 Tucannon RL-0065 GTATCC  03EK-28 Tucannon RL-0065 TCCTGC  03EK-29 Tucannon RL-0065 TTCCGT  03EK-3 Tucannon RL-0065 AATGAG  03EK-32 Tucannon RL-0065 ACAGCG  03EK-38 Tucannon RL-0065 ATAGTA  03EK-40 Tucannon RL-0065 CCCTAA  03EK-6 Tucannon RL-0065 AGTAGG  03EK-7 Tucannon RL-0065 CCATTT  03EK-93 Tucannon RL-0065 CTGCGA  03EK-96 Tucannon RL-0065 GCCGTA  03EK-98 Tucannon RL-0065 GTCATC  03EK-99 Tucannon RL-0065 TCGATT  03EL-100 Tucannon RL-0065 TTTGTC  03EL-13 Tucannon RL-0065 CCGAGG  03EL-14 Tucannon RL-0065 CTGGTT  03EL-16 Tucannon RL-0065 GCGACC  03EL-17 Tucannon RL-0065 GTGCCT  03EL-21 Tucannon RL-0065 TCGCCA  03EL-23 Tucannon RL-0065 TTGAGC  03EL-24 Tucannon RL-0065 ACCATG  03EL-25 Tucannon RL-0065 ATGCAC  03EL-26 Tucannon RL-0065 CCGCAT  03EL-27 Tucannon RL-0065 CTTATG  03EL-3 Tucannon RL-0065 TTCTAG  03EL-30 Tucannon RL-0065 GCGCTG  03EL-31 Tucannon RL-0065 GTGTAA  03EL-4 Tucannon RL-0065 ACATAC  03EL-46 Tucannon RL-0065 TCGGAC  03EL-54 Tucannon RL-0065 TTTAAT  03EL-57 Tucannon RL-0065 ACCCCC  03EL-58 Tucannon RL-0065 ATGTTG  03EL-59 Tucannon RL-0065 CCTAAC  03EL-64 Tucannon RL-0065 CTTTGC  03EL-7 Tucannon RL-0065 ATCAAA  03EL-87 Tucannon RL-0065 GCTCAA  03EL-89 Tucannon RL-0065 GTTGGA  03EL-90 Tucannon RL-0065 TCTCGG