title: GSM2677644: ChIP-seq_S10P of shMSK1 cells; Homo sapiens; ChIP-Seq
library_construction_protocol: DBM shCTRL and shMSK1 cells were trypsinised and crosslinked in 1% formaldehyde for 10 min at RT. rosslinking was quenched with 0.125 M glycine. Pelleted cells were lysed in 1 ml ChIP buffer (150 mM NaCl, 10 mM Tris-HCl, 5 mM EDTA, 1% SDS, 0.5 mM DTT and 1% Triton X-100) and sonicated for 20 min in a Bioruptor Pico (Diagenode). Sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (E7370L) following manufacturer's instruction. Antibodies used for ChIP were: H3S10p (Abcam ab5176), H3S28p (Sigma-Aldrich H9908)
attributes.tag: GEO Accession
attributes.value: GSM2677644