title: RAD-Seq in O. tshawytscha from Yankee Fork River, ID & Twisp River, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsYF07-S7S1-01 Jordan Creek Yankee Fork RL-0066 TATCAC  OtsYF07-S7S1-07 Jordan Creek Yankee Fork RL-0066 TGGGGA  OtsYF07-S1S2-01 Mainstem Yankee Fork RL-0066 AAACGG  OtsYF07-S1S2-03 Mainstem Yankee Fork RL-0066 ACTCTT  OtsYF07-S1S2-04 Mainstem Yankee Fork RL-0066 ATTCCG  OtsYF07-S1S2-06 Mainstem Yankee Fork RL-0066 CGAGGC  OtsYF07-S1S2-07 Mainstem Yankee Fork RL-0066 GAAATG  OtsYF07-S1S2-09 Mainstem Yankee Fork RL-0066 GGACTT  OtsYF07-S1S3-02 Mainstem Yankee Fork RL-0066 TAAGCT  OtsYF07-S1S3-10 Mainstem Yankee Fork RL-0066 TCTTCT  OtsYF07-S1S3-11 Mainstem Yankee Fork RL-0066 AACGTT  OtsYF07-S2S1-04 Mainstem Yankee Fork RL-0066 ACTGGC  OtsYF07-S2S1-06 Mainstem Yankee Fork RL-0066 CAAAAA  OtsYF07-S2S1-08 Mainstem Yankee Fork RL-0066 CGCAGA  OtsYF07-S2S1-10 Mainstem Yankee Fork RL-0066 GAACCA  OtsYF07-S2S1-11 Mainstem Yankee Fork RL-0066 GGCAAG  OtsYF07-S2S1-14 Mainstem Yankee Fork RL-0066 TAATTC  OtsYF07-S2S2-09 Mainstem Yankee Fork RL-0066 TGAACC  OtsYF07-S2S3-03 Mainstem Yankee Fork RL-0066 AACTGA  OtsYF07-S2S3-05 Mainstem Yankee Fork RL-0066 AGCCAT  OtsYF07-S2S3-19 Mainstem Yankee Fork RL-0066 CAATCG  OtsYF07-S2S3-21 Mainstem Yankee Fork RL-0066 CGCGTG  OtsYF07-S3S1-04 Mainstem Yankee Fork RL-0066 GACGAC  OtsYF07-S3S1-05 Mainstem Yankee Fork RL-0066 GGGCGC  OtsYF07-S3S3-02 Mainstem Yankee Fork RL-0066 TACACA  OtsYF07-S3S3-03 Mainstem Yankee Fork RL-0066 TGACAA  OtsYF07-S3S3-07 Mainstem Yankee Fork RL-0066 AAGACG  OtsYF07-S3S3-10 Mainstem Yankee Fork RL-0066 AGCGCA  OtsYF07-S3S3-14 Mainstem Yankee Fork RL-0066 CACCTC  OtsYF07-S4S2-04 Mainstem Yankee Fork RL-0066 CGGTCC  OtsYF07-S4S2-06 Mainstem Yankee Fork RL-0066 GACTCT  OtsYF07-S4S3-14 Mainstem Yankee Fork RL-0066 GGGGCG  OtsYF07-S4S3-15 Mainstem Yankee Fork RL-0066 TACGGG  Ots2001-TWI-0049 Twisp RL-0066 AATGAG  Ots2001-TWI-0059 Twisp RL-0066 AGTAGG  Ots2001-TWI-0279 Twisp RL-0066 CCATTT  Ots2001-TWI-0301 Twisp RL-0066 CTACAG  Ots2001-TWI-0311 Twisp RL-0066 GCAGAT  Ots2001-TWI-0312 Twisp RL-0066 GTAAGT  Ots2001-TWI-0321 Twisp RL-0066 TCAAAG  Ots2001-TWI-0327 Twisp RL-0066 TTATGA  Ots2001-TWI-0343 Twisp RL-0066 ACAAGA  Ots2001-TWI-0358 Twisp RL-0066 AGTTAA  Ots2001-TWI-0360 Twisp RL-0066 CCCGGT  Ots2001-TWI-0454 Twisp RL-0066 CTCGCC  Ots2001-TWI-0688 Twisp RL-0066 GCATGG  Ots2001-TWI-0696 Twisp RL-0066 GTATCC  Ots2001-TWI-0721 Twisp RL-0066 TCCTGC  Ots2001-TWI-0751 Twisp RL-0066 ACAGCG  Ots2001-TWI-0829 Twisp RL-0066 ATAGTA  Ots2001-TWI-0835 Twisp RL-0066 CCCTAA  Ots2001-TWI-0853 Twisp RL-0066 CTGCGA  Ots2001-TWI-0859 Twisp RL-0066 GCCGTA  Ots2001-TWI-0869 Twisp RL-0066 GTCATC  Ots2001-TWI-0875 Twisp RL-0066 TCGATT  Ots2001-TWI-0877 Twisp RL-0066 TTCTAG  Ots2001-TWI-0882 Twisp RL-0066 ACATAC  Ots2001-TWI-0883 Twisp RL-0066 ATCAAA  Ots2001-TWI-0895 Twisp RL-0066 CCGAGG  Ots2001-TWI-0919 Twisp RL-0066 CTGGTT  Ots2001-TWI-0930 Twisp RL-0066 GCGACC  Ots2001-TWI-0978 Twisp RL-0066 GTGCCT  Ots2001-TWI-1006 Twisp RL-0066 TCGCCA  Ots2001-TWI-1052 Twisp RL-0066 TTGAGC  Ots2001-TWI-1064 Twisp RL-0066 ACCATG  Ots2001-TWI-1071 Twisp RL-0066 ATGCAC  Ots2001-TWI-1149 Twisp RL-0066 CCGCAT  Ots2001-TWI-1159 Twisp RL-0066 CTTATG  Ots2001-TWI-1160 Twisp RL-0066 GCGCTG  Ots2001-TWI-1168 Twisp RL-0066 GTGTAA  Ots2001-TWI-1287 Twisp RL-0066 TCGGAC  Ots2001-TWI-1291 Twisp RL-0066 TTTAAT  Ots2001-TWI-1293 Twisp RL-0066 ACCCCC  Ots2001-TWI-1299 Twisp RL-0066 ATGTTG  Ots2001-TWI-1374 Twisp RL-0066 CCTAAC  Ots2001-TWI-1377 Twisp RL-0066 CTTTGC  Ots2001-TWI-1389 Twisp RL-0066 GCTCAA  Ots2001-TWI-1416 Twisp RL-0066 GTTGGA  Ots2001-TWI-2792 Twisp RL-0066 TCTCGG  Ots2001-TWI-3311 Twisp RL-0066 TTTGTC  OtsYF07-S6S1-02 West Fork Yankee Fork RL-0066 TGCCCG  OtsYF07-S6S1-05 West Fork Yankee Fork RL-0066 AAGCTA  OtsYF07-S6S1-06 West Fork Yankee Fork RL-0066 AGGGTC  OtsYF07-S6S1-12 West Fork Yankee Fork RL-0066 CAGGCA  OtsYF07-S6S1-13 West Fork Yankee Fork RL-0066 CGTCTA  OtsYF07-S6S1-15 West Fork Yankee Fork RL-0066 GAGAGA  OtsYF07-S6S1-18 West Fork Yankee Fork RL-0066 GGTACA  OtsYF07-S6S1-21 West Fork Yankee Fork RL-0066 TAGTAT  OtsYF07-S6S1-24 West Fork Yankee Fork RL-0066 TGCTTA  OtsYF07-S6S1-30 West Fork Yankee Fork RL-0066 AATATC  OtsYF07-S6S1-34 West Fork Yankee Fork RL-0066 AGGTGT  OtsYF07-S6S2-05 West Fork Yankee Fork RL-0066 CATACT  OtsYF07-S6S3-01 West Fork Yankee Fork RL-0066 CGTGAT  OtsYF07-S6S3-03 West Fork Yankee Fork RL-0066 GATCGT  OtsYF07-S6S3-04 West Fork Yankee Fork RL-0066 GGTTTG