title: RAD-Seq in O. tshawytscha from Lyons Ferry Hatchery, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsPBT11-LF-1634 Lyons Ferry Hatchery RL-0043 AAACGG  OtsPBT11-LF-1701 Lyons Ferry Hatchery RL-0043 ACTCTT  OtsPBT11-LF-1797 Lyons Ferry Hatchery RL-0043 ATTCCG  OtsPBT11-LF-1819 Lyons Ferry Hatchery RL-0043 CGAGGC  OtsPBT11-LF-1901 Lyons Ferry Hatchery RL-0043 GAAATG  OtsPBT11-LF-1964 Lyons Ferry Hatchery RL-0043 GGACTT  OtsPBT11-LF-2011 Lyons Ferry Hatchery RL-0043 TAAGCT  OtsPBT11-LF-2025 Lyons Ferry Hatchery RL-0043 TCTTCT  OtsPBT11-LF-2036 Lyons Ferry Hatchery RL-0043 AACGTT  OtsPBT11-LF-2046 Lyons Ferry Hatchery RL-0043 ACTGGC  OtsPBT11-LF-2064 Lyons Ferry Hatchery RL-0043 CAAAAA  OtsPBT11-LF-2094 Lyons Ferry Hatchery RL-0043 CGCAGA  OtsPBT11-LF-2122 Lyons Ferry Hatchery RL-0043 GAACCA  OtsPBT11-LF-2164 Lyons Ferry Hatchery RL-0043 GGCAAG  OtsPBT11-LF-2176 Lyons Ferry Hatchery RL-0043 TAATTC  OtsPBT11-LF-2191 Lyons Ferry Hatchery RL-0043 TGAACC  OtsPBT11-LF-2273 Lyons Ferry Hatchery RL-0043 AACTGA  OtsPBT11-LF-2326 Lyons Ferry Hatchery RL-0043 AGCCAT  OtsPBT11-LF-2330 Lyons Ferry Hatchery RL-0043 CAATCG  OtsPBT11-LF-2528 Lyons Ferry Hatchery RL-0043 CGCGTG  OtsPBT11-LF-2539 Lyons Ferry Hatchery RL-0043 GACGAC  OtsPBT11-LF-2568 Lyons Ferry Hatchery RL-0043 GGGCGC  OtsPBT11-LF-2637 Lyons Ferry Hatchery RL-0043 TACACA  OtsPBT11-LF-2653 Lyons Ferry Hatchery RL-0043 TGACAA  OtsPBT11-LF-2682 Lyons Ferry Hatchery RL-0043 AAGACG  OtsPBT11-LF-2685 Lyons Ferry Hatchery RL-0043 AGCGCA  OtsPBT11-LF-2711 Lyons Ferry Hatchery RL-0043 CACCTC  OtsPBT11-LF-2722 Lyons Ferry Hatchery RL-0043 CGGTCC  OtsPBT11-LF-2765 Lyons Ferry Hatchery RL-0043 GACTCT  OtsPBT11-LF-2822 Lyons Ferry Hatchery RL-0043 GGGGCG  OtsPBT11-LF-2893 Lyons Ferry Hatchery RL-0043 TACGGG  OtsPBT11-LF-2898 Lyons Ferry Hatchery RL-0043 TGCCCG  OtsPBT11-LF-2926 Lyons Ferry Hatchery RL-0043 AAGCTA  OtsPBT11-LF-2940 Lyons Ferry Hatchery RL-0043 AGGGTC  OtsPBT11-LF-3169 Lyons Ferry Hatchery RL-0043 CAGGCA  OtsPBT11-LF-3201 Lyons Ferry Hatchery RL-0043 CGTCTA  OtsPBT11-LF-3309 Lyons Ferry Hatchery RL-0043 GAGAGA  OtsPBT11-LF-3324 Lyons Ferry Hatchery RL-0043 GGTACA  OtsPBT11-LF-3335 Lyons Ferry Hatchery RL-0043 TAGTAT  OtsPBT11-LF-3339 Lyons Ferry Hatchery RL-0043 TGCTTA  OtsPBT11-LF-3428 Lyons Ferry Hatchery RL-0043 AATATC  OtsPBT11-LF-3431 Lyons Ferry Hatchery RL-0043 AGGTGT  OtsPBT11-LF-3532 Lyons Ferry Hatchery RL-0043 CATACT  OtsPBT11-LF-3536 Lyons Ferry Hatchery RL-0043 CGTGAT  OtsPBT11-LF-3537 Lyons Ferry Hatchery RL-0043 GATCGT  OtsPBT11-LF-3615 Lyons Ferry Hatchery RL-0043 GGTTTG  OtsPBT11-LF-3643 Lyons Ferry Hatchery RL-0043 TATCAC  OtsPBT11-LF-3659 Lyons Ferry Hatchery RL-0043 CCCGGT