title: 16S amplicon-seq of  viral hepatitis UKKMD021
design: DNA from fecal  samples was isolated using the RNeasy Power Microbiome Kit (Qiagen, Germany).  Seven of the 9 variable bacterial 16S rDNA regions (pool 1: V2, V4 and V8;  pool 2: V3, V6/7 and V9) were amplified with the Ion 16S Metagenomics Kit  (Thermo Fisher Scienctific, USA) utilizing two primer pools. Amplicons were  pooled and cleaned using the NucleoMag NGS Clean-up (Macherey-Nagel,  Germany), followed by library preparation with the Ion Plus Fragment Library  Kit (Thermo Fisher Scienctific, USA).