title: RAD-Seq in O. tshawytscha from Marsh Creek, ID
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsMarsh00-0017 Marsh Creek RL-0041 AATGAG  OtsMarsh00-0019 Marsh Creek RL-0041 AGTAGG  OtsMarsh01-0637 Marsh Creek RL-0041 CCATTT  OtsMarsh01-0640 Marsh Creek RL-0041 CTACAG  OtsMarsh01-0643 Marsh Creek RL-0041 GCAGAT  OtsMarsh01-0644 Marsh Creek RL-0041 GTAAGT  OtsMarsh01-0645 Marsh Creek RL-0041 TCAAAG  OtsMarsh01-0694 Marsh Creek RL-0041 TTATGA  OtsMarsh01-0697 Marsh Creek RL-0041 ACAAGA  OtsMarsh01-0698 Marsh Creek RL-0041 AGTTAA  OtsMarsh01-0700 Marsh Creek RL-0041 CCCGGT  OtsMarsh01-0701 Marsh Creek RL-0041 CTCGCC  OtsMarsh01-0703 Marsh Creek RL-0041 GCATGG  OtsMarsh01-0704 Marsh Creek RL-0041 GTATCC  OtsMarsh02-0426 Marsh Creek RL-0041 TCCTGC  OtsMarsh02-0428 Marsh Creek RL-0041 TTCCGT  OtsMarsh02-0430 Marsh Creek RL-0041 ACAGCG  OtsMarsh02-0433 Marsh Creek RL-0041 ATAGTA  OtsMarsh02-0440 Marsh Creek RL-0041 CCCTAA  OtsMarsh02-0445 Marsh Creek RL-0041 CTGCGA  OtsMarsh02-0446 Marsh Creek RL-0041 GCCGTA  OtsMarsh02-0447 Marsh Creek RL-0041 GTCATC  OtsMarsh02-0449 Marsh Creek RL-0041 TCGATT  OtsMarsh02-0450 Marsh Creek RL-0041 TTCTAG  OtsMarsh02-0466 Marsh Creek RL-0041 ACATAC  OtsMarsh02-0467 Marsh Creek RL-0041 ATCAAA  OtsMarsh02-0472 Marsh Creek RL-0041 CCGAGG  OtsMarsh02-0473 Marsh Creek RL-0041 CTGGTT  OtsMarsh03-0147 Marsh Creek RL-0041 GCGACC  OtsMarsh03-0154 Marsh Creek RL-0041 GTGCCT  OtsMarsh03-0156 Marsh Creek RL-0041 TCGCCA  OtsMarsh03-0157 Marsh Creek RL-0041 TTGAGC  OtsMarsh07-0001 Marsh Creek RL-0041 ACCATG  OtsMarsh07-0002 Marsh Creek RL-0041 ATGCAC  OtsMarsh07-0005 Marsh Creek RL-0041 CCGCAT  OtsMarsh07-0006 Marsh Creek RL-0041 CTTATG  OtsMarsh07-0010 Marsh Creek RL-0041 GCGCTG  OtsMarsh07-0011 Marsh Creek RL-0041 GTGTAA  OtsMarsh07-0013 Marsh Creek RL-0041 TCGGAC  OtsMarsh08-0003 Marsh Creek RL-0041 TTTAAT  OtsMarsh08-0009 Marsh Creek RL-0041 ACCCCC  OtsMarsh08-0010 Marsh Creek RL-0041 ATGTTG  OtsMarsh08-0012 Marsh Creek RL-0041 CCTAAC  OtsMarsh08-0016 Marsh Creek RL-0041 CTTTGC  OtsMarsh08-0017 Marsh Creek RL-0041 GCTCAA  OtsMarsh08-0018 Marsh Creek RL-0041 GTTGGA  OtsMarsh98-0001 Marsh Creek RL-0041 TCTCGG  OtsMarsh98-0002 Marsh Creek RL-0041 TTTGTC