title: GSM4626653: ChIP-seq BL-CAP-C F123 CTCF-AID KO Pol2-S5P; Mus musculus; ChIP-Seq
library_construction_protocol: For all ChIP-Seq experiment, after formaldehyde crosslinking, chromatins were sheared by sonication and DNA bound with protein of interest was enriched by antibodies. DNA protein complexes were revese crosslinked and extracted by phenol:chloroform. DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment ( exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation, DNA was PCR amplified with Illumina primers for 11 cycles and library fragments of ~300 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4626653