title: Dispersal sources of bacteria to lakes: Sample Feb_25_SedS
design: DNA was extracted using the PowerSoil DNA Isolation Kit (MO BIO Laboratories Inc., USA). V3-V4 regions of bacterial 16S rRNA genes were PCR amplified using forward primer 341 (5'-CCTACGGGNGGCWGCAG-3') and individually bar-coded reverse primers 805 (5'- GACTACHVGGGTATCTAATCC-3'). The resulting  Biosamples were sequenced using Roche/454 GS FLX Titanium technology.