title: WGS of Homo Sapiens: Blood: Sample UCSFFS.0800
design: The DNA libraries were prepared according to Illumina TruSeq(TM) DNA Sample Prep protocol. Total cellular DNA was sheared to sizes between 200 and 800 base pairs by using a Covaris S2 sonicator according to the Illumina TruSeq DNA protocol and then libraries were prepared using the Illumina TruSeq(TM) DNA Sample Prep Kit on Tecan Freedom EVO 200 Automated Liquid Handling System. Libraries were size selected for insert fragments around 300 base pairs using Pippin Prep automatic DNA size selection system (Sage Science). Libraries were analyzed and quantified using a LabChip GX automated electrophoresis system (Caliper) and diluted to 15 nM concentration. The paired-end sequencing (2 x 100 cycles) was performed on HiSeq2000 sequencers (Illumina). Initially each library was sequenced on a single lane of a flow cell. The measured concentration of DNA in a library is an unreliable predictor of the optimal amount of DNA that should be loaded on a flow cell. During the course of this experiment we converted to a strategy where 24 samples were pooled and run on multiple lanes of a flow cell such that the optimal amount of DNA could be determined to enable collection of the greatest amount of quality sequence per lane.
attributes.tag: alignment_software
attributes.value: BWA