title: WGS of Vibrio tetraodonis: isolated from fish skin
design: Libraries were built by using the Nextera XT Sample Preparation Kit (Illumina). DNA was simultaneously fragmented and tagged with sequencing adapters. The library size distribution was accessed using the 2100 Bioanalyzer and the High Sensitivity DNA Kit (Agilent). The accurate quantification of the library was accomplished using the 7500 Real Time PCR (Applied Biosystems) and the KAPA Library Quantification Kit (Kapabiosystems). Paired-end (2250 bp) sequencing was performed on a MiSeq (Illumina) using the MiSeq reagent kit v2 (500 cycles). The complex DNA was sequenced using Illumina MiSeq in the sequencing unity of SAGE, COPPE, UFRJ, Brazil.