title: GSM4503607: WT1_podocyte_mTmG_PBS_rep3; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin from isolated glomeruli and isolated podocytes were prepared from 7-8-week old BALB/cJ, mT/mG-Nphs2cre and WT1 flox/flox/Nphs2iCRE mice. For, WT1 ChIP assays, we used per immunoprecipitation either isolated glomeruli from two kidneys or 10 million of immortalized mouse podocytes (four independent experiments were performed per condition for BALB/cJ mice and three independent experiments for mT/mG Nphs2cre mice and immortalized mouse podocytes). Samples were fixed with 1% formaldehyde in PBS for 5 min at room temperature before termination with 0.125 M glycine. Cells were then lysed in sonication buffer as previously described (4). Cross-linked chromatin was sonicated to obtain DNA fragments of 200–600 bp. Immunoprecipitations were performed as previously. Libraries for sequencing were prepared using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4503607