title: GSM2415917: Untreated Replicate #2; Mus musculus; RNA-Seq
library_construction_protocol: To generate libraries for RNA-seq, polyA+ RNA was was extracted using the Dynabeads mRNA Direct kit (Ambion) according to the manufacturer’s instructions. RNA-seq libraries were made using a ScriptSeq v2 RNA-seq Library Preparation Kit from Epicentre (now Illumina). The quantity and quality of the libraries were assessed by BioAnalyzer (Agilent) and qPCR (Kapa Biosystems). The multiplexed libraries were pooled and sequenced on a single lane on the Illumina NextSeq 500 platform
attributes.tag: GEO Accession
attributes.value: GSM2415917