title: GSM3753506: Ribo-Seq wt+HA (RP_wt_HA), run 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells washed with PBS supplemented with cycloheximide 100 µg/ml, lysed in polysome lysis buffer and cleared by centrifugation at 20,000 g for 10 min. Library was prepared with TruSeq Ribo Profile Kit. rRNA depletion was performed using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat).