title: GSM3168501: RNA-seq WT 1 SB2h; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA purified from d2.5 EBs with Trim33 WT ESCs in C57Bl/6J background (as described (Xi et al., 2008) was quantified by Ribogreen and quality assessed by Agilent BioAnalyzer 2000 The RNA-seq library preparation and sequencing was performed as descripted before (Wang et al.,2017) After scraped from plate, embryonic stem cells were washed with PBS and incubated in lysis buffer.
attributes.tag: GEO Accession
attributes.value: GSM3168501