title: Miseq-18SrRNA_Rhizosphere_Roots_AMF229
design: 250 l of dry pulverized root fragments were used for total genomic DNA extraction using the Nucleospin@ soil kit (Macherey-Nagel). Partial 18S rRNA gene was then amplified in a PCR reaction using the ML1/ML2 primer pair, followed by a second round with in-house primers containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. The amplicons were purified using the Nucleomag@ NGS clean-up and size select kit (Macherey-Nagel). Finally, amplicons were pooled at equimolar concentration and sent for 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated and quality checked, using the Mothur and Qiime softwares