title: Illumina HiSeq 2500 paired end sequencing; RNA-seq analysis of two cell line models of lung diseases in Project 3 of Open Targets - Epigenomes of Cell Lines
design: RNA-seq analysis of two cell line models of lung diseases in Project 3 of Open Targets - Epigenomes of Cell Lines
library_construction_protocol: F12K + 10% FBS Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer’s protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced
attributes.tag: Experimental Factor: A549
attributes.value: cell line