title: GSM3937627: Cytokines1; Homo sapiens; RNA-Seq
library_construction_protocol: EndoC cells were treated with or without 1 x Cytokines for 24h. Cells were collected, washed by PBS. Cells were lysed and RNA were extracted using RNeasy Mini Kit (QIAGEN, Cat. # 74104).   NEBNext Poly(A) Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7765) were used to generate libraries.  The BioAnalyzer is used for quality control of the libraries to ensure adequate concentration and appropriate fragment size.  The resulting library insert size is 200bp-500bp with a median size around 300bp. Libraries were uniquely barcoded and pooled for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM3937627