title: GSM3753207: Biosample_8606_4132_58665_HYL2CBGX2_K562_InSituHiC_HS_1_CGATGT; Homo sapiens; Hi-C
library_construction_protocol: Cells were crosslinked with 1% formaldehyde for 10 min, quenched and permeabilized. Nuclei were digested with MboI and ends were biotinylated with biotin-14-dATP followed by in-situ ligation. Crosslinks were reversed and DNA was purified, sonicated and pulled down by streptavidin beads. DNA was end repaired with T4 DNA polymerase, DNA polymerase I large (Klenow) polymerase and T4 polynucleotide kinase. DNA ends were adenylated by dATP and Klenow (3'-5' exo-) polymerase. Illumina Truseq adapters were ligated to the DNA ends and PCR amplified for 10 cycles. Library was purified with Qiaquick PCR purfication kit and sequenced on Illumina Nextseq 500.