title: RAD-Seq in O. tshawytscha from Naches River, WA & Deschutes River, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  Ots_11DRASCHF-0015 Deschutes River (Above Sherars Falls) RL-0047 AATGAG  Ots_11DRASCHF-0016 Deschutes River (Above Sherars Falls) RL-0047 AGTAGG  Ots_11DRASCHF-0021 Deschutes River (Above Sherars Falls) RL-0047 CCATTT  Ots_11DRASCHF-0023 Deschutes River (Above Sherars Falls) RL-0047 CTACAG  Ots_11DRASCHF-0024 Deschutes River (Above Sherars Falls) RL-0047 GCAGAT  Ots_11DRASCHF-0026 Deschutes River (Above Sherars Falls) RL-0047 GTAAGT  Ots_11DRASCHF-0029 Deschutes River (Above Sherars Falls) RL-0047 TCAAAG  Ots_11DRASCHF-0030 Deschutes River (Above Sherars Falls) RL-0047 TTATGA  Ots_11DRASCHF-0032 Deschutes River (Above Sherars Falls) RL-0047 ACAAGA  Ots_11DRASCHF-0033 Deschutes River (Above Sherars Falls) RL-0047 AGTTAA  Ots_11DRASCHF-0035 Deschutes River (Above Sherars Falls) RL-0047 CCCGGT  Ots_11DRASCHF-0039 Deschutes River (Above Sherars Falls) RL-0047 CTCGCC  Ots_11DRASCHF-0040 Deschutes River (Above Sherars Falls) RL-0047 GCATGG  Ots_11DRASCHF-0041 Deschutes River (Above Sherars Falls) RL-0047 GTATCC  Ots_11DRASCHF-0042 Deschutes River (Above Sherars Falls) RL-0047 TCCTGC  Ots_11DRASCHF-0043 Deschutes River (Above Sherars Falls) RL-0047 TTCCGT  Ots_11DRASCHF-0049 Deschutes River (Above Sherars Falls) RL-0047 ACAGCG  Ots_11DRASCHF-0052 Deschutes River (Above Sherars Falls) RL-0047 ATAGTA  Ots_11DRASCHF-0054 Deschutes River (Above Sherars Falls) RL-0047 CCCTAA  Ots_11DRASCHF-0056 Deschutes River (Above Sherars Falls) RL-0047 CTGCGA  Ots_11DRASCHF-0058 Deschutes River (Above Sherars Falls) RL-0047 GCCGTA  Ots_11DRASCHF-0059 Deschutes River (Above Sherars Falls) RL-0047 GTCATC  Ots_11DRASCHF-0060 Deschutes River (Above Sherars Falls) RL-0047 TCGATT  Ots_11DRASCHF-0061 Deschutes River (Above Sherars Falls) RL-0047 TTCTAG  Ots_11DRASCHF-0063 Deschutes River (Above Sherars Falls) RL-0047 ACATAC  Ots_11DRASCHF-0069 Deschutes River (Above Sherars Falls) RL-0047 ATCAAA  Ots_11DRASCHF-0072 Deschutes River (Above Sherars Falls) RL-0047 CCGAGG  Ots_11DRASCHF-0074 Deschutes River (Above Sherars Falls) RL-0047 CTGGTT  Ots_11DRASCHF-0076 Deschutes River (Above Sherars Falls) RL-0047 GCGACC  Ots_11DRASCHF-0078 Deschutes River (Above Sherars Falls) RL-0047 GTGCCT  Ots_11DRASCHF-0079 Deschutes River (Above Sherars Falls) RL-0047 TCGCCA  Ots_11DRASCHF-0080 Deschutes River (Above Sherars Falls) RL-0047 TTGAGC  Ots_11DRASCHF-0081 Deschutes River (Above Sherars Falls) RL-0047 ACCATG  Ots_11DRASCHF-0085 Deschutes River (Above Sherars Falls) RL-0047 ATGCAC  Ots_11DRASCHF-0088 Deschutes River (Above Sherars Falls) RL-0047 CCGCAT  Ots_11DRASCHF-0089 Deschutes River (Above Sherars Falls) RL-0047 CTTATG  Ots_11DRASCHF-0090 Deschutes River (Above Sherars Falls) RL-0047 GCGCTG  Ots_11DRASCHF-0091 Deschutes River (Above Sherars Falls) RL-0047 GTGTAA  Ots_11DRASCHF-0092 Deschutes River (Above Sherars Falls) RL-0047 TCGGAC  Ots_11DRASCHF-0096 Deschutes River (Above Sherars Falls) RL-0047 TTTAAT  Ots_11DRASCHF-0097 Deschutes River (Above Sherars Falls) RL-0047 ACCCCC  Ots_11DRASCHF-0098 Deschutes River (Above Sherars Falls) RL-0047 ATGTTG  Ots_11DRASCHF-0099 Deschutes River (Above Sherars Falls) RL-0047 CCTAAC  Ots_11DRASCHF-0100 Deschutes River (Above Sherars Falls) RL-0047 CTTTGC  Ots_11DRASCHF-0102 Deschutes River (Above Sherars Falls) RL-0047 GCTCAA  Ots_11DRASCHF-0104 Deschutes River (Above Sherars Falls) RL-0047 GTTGGA  Ots_11DRASCHF-0106 Deschutes River (Above Sherars Falls) RL-0047 TCTCGG  Ots_11DRASCHF-0107 Deschutes River (Above Sherars Falls) RL-0047 TTTGTC  OtsNach04BI-02 Naches RL-0047 AGCGCA  OtsNach04BI-03 Naches RL-0047 CACCTC  OtsNach04BI-04 Naches RL-0047 CGGTCC  OtsNach04BI-06 Naches RL-0047 GACTCT  OtsNach04BI-07 Naches RL-0047 GGGGCG  OtsNach04BI-08 Naches RL-0047 TACGGG  OtsNach04BI-13 Naches RL-0047 TGCCCG  OtsNach04BI-14 Naches RL-0047 AAGCTA  OtsNach04BI-15 Naches RL-0047 AGGGTC  OtsNach04BI-16 Naches RL-0047 CAGGCA  OtsNach04BI-17 Naches RL-0047 CGTCTA  OtsNach04BI-18 Naches RL-0047 GAGAGA  OtsNach04BI-19 Naches RL-0047 GGTACA  OtsNach04BI-21 Naches RL-0047 TAGTAT  OtsNach04BI-22 Naches RL-0047 TGCTTA  OtsNach04BI-23 Naches RL-0047 AATATC  OtsNach04BI-24 Naches RL-0047 AGGTGT  OtsNach04BI-32 Naches RL-0047 CATACT  OtsNach04BI-33 Naches RL-0047 CGTGAT  OtsNach04BI-34 Naches RL-0047 GATCGT  OtsNach04BI-35 Naches RL-0047 GGTTTG  OtsNach04BI-37 Naches RL-0047 TATCAC  OtsNach04BI-38 Naches RL-0047 TGGGGA  OtsNach04EM-07 Naches RL-0047 AAACGG  OtsNach04EM-08 Naches RL-0047 ACTCTT  OtsNach04EM-100 Naches RL-0047 AAGACG  OtsNach04EM-11 Naches RL-0047 ATTCCG  OtsNach04EM-19 Naches RL-0047 GAAATG  OtsNach04EM-21 Naches RL-0047 GGACTT  OtsNach04EM-23 Naches RL-0047 TAAGCT  OtsNach04EM-24 Naches RL-0047 TCTTCT  OtsNach04EM-26 Naches RL-0047 AACGTT  OtsNach04EM-27 Naches RL-0047 ACTGGC  OtsNach04EM-29 Naches RL-0047 CAAAAA  OtsNach04EM-30 Naches RL-0047 CGCAGA  OtsNach04EM-43 Naches RL-0047 GAACCA  OtsNach04EM-57 Naches RL-0047 GGCAAG  OtsNach04EM-75 Naches RL-0047 TAATTC  OtsNach04EM-76 Naches RL-0047 TGAACC  OtsNach04EM-77 Naches RL-0047 AACTGA  OtsNach04EM-84 Naches RL-0047 AGCCAT  OtsNach04EM-85 Naches RL-0047 CAATCG  OtsNach04EM-86 Naches RL-0047 CGCGTG  OtsNach04EM-88 Naches RL-0047 GACGAC  OtsNach04EM-90 Naches RL-0047 GGGCGC  OtsNach04EM-97 Naches RL-0047 TACACA  OtsNach04EM-99 Naches RL-0047 TGACAA