title: RAD-Seq in O. tshawytscha from  Nason Creek, Chiwawa River & White River
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsWENcr07-060107-001 Chiwawa River RL0008 GACGAC  OtsWENcr07-062007-001 Chiwawa River RL0008 GGGCGC  OtsWENcr07-062207-001 Chiwawa River RL0008 TACACA  OtsWENcr07-062807-001 Chiwawa River RL0008 TGACAA  OtsWENcr07-063007-001 Chiwawa River RL0008 AAGACG  OtsWENcr07-070407-001 Chiwawa River RL0008 AGCGCA  OtsWENcr07-070507-001 Chiwawa River RL0008 CACCTC  OtsWENcr07-070807-001 Chiwawa River RL0008 CGGTCC  OtsWENcr07-071107-001 Chiwawa River RL0008 GACTCT  OtsWENcr07-071507-001 Chiwawa River RL0008 GGGGCG  OtsWENnr05-052405-001 Nason Creek RL0008 AAACGG  OtsWENnr05-060105-001 Nason Creek RL0008 ACTCTT  OtsWENnr05-060705-001 Nason Creek RL0008 ATTCCG  OtsWENnr05-061105-001 Nason Creek RL0008 CGAGGC  OtsWENnr05-061505-001 Nason Creek RL0008 GAAATG  OtsWENnr05-061805-001 Nason Creek RL0008 GGACTT  OtsWENnr05-062005-001 Nason Creek RL0008 TAAGCT  OtsWENnr05-062405-001 Nason Creek RL0008 TCTTCT  OtsWENnr05-063005-001 Nason Creek RL0008 AACGTT  OtsWENnr05-071205-001 Nason Creek RL0008 ACTGGC  OtsWENnr09-060509-001 Nason Creek RL0008 TACGGG  OtsWENnr09-062009-001 Nason Creek RL0008 TGCCCG  OtsWENnr09-062309-001 Nason Creek RL0008 AAGCTA  OtsWENwr05-052505-001 White River RL0008 CAAAAA  OtsWENwr05-061605-001 White River RL0008 CGCAGA  OtsWENwr05-061805-001 White River RL0008 GAACCA  OtsWENwr05-062405-001 White River RL0008 GGCAAG  OtsWENwr05-062605-001 White River RL0008 TAATTC  OtsWENwr05-062705-001 White River RL0008 TGAACC  OtsWENwr05-070105-001 White River RL0008 AACTGA  OtsWENwr05-070405-001 White River RL0008 AGCCAT  OtsWENwr05-070705-001 White River RL0008 CAATCG  OtsWENwr05-071705-001 White River RL0008 CGCGTG  OtsWENwr09-061609-001 White River RL0008 AGGGTC  OtsWENwr09-062309-001 White River RL0008 CAGGCA  OtsWENwr09-062509-001 White River RL0008 CGTCTA