title: GSM4678156: PROseq_K562_s_pC12p5_rep2; Homo sapiens; OTHER
library_construction_protocol: After treatments, the cells were harvested by centrifugation, and washed twice with PBS. Chromatin was isolated as in Chu et al., 2018, Nature Genetics, 50: 1553–1564 and run-on reactions performed with biotinylated nucleotides as described by Kwak et al., 2013, Science, 339:950-953. The run-on reaction was conducted in the presence of chromatin from S2 cells, which provides an invariant whole-genome spike-in control between samples. The libraries were generated using TruSeq small-RNA adaptors. Half of the libraries were amplified for 12 cycles, after which the libraries were purified and size-selected with Ampure XP beads. Distinct libraries were combined for HiSeq (Illumina, Novogene) sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4678156