title: Comparative faecal microbiota of dogs with and without calcium oxalate stones
design: Fresh faecal samples were collected from healthy dogs (n = 5) and dogs with CaOx stones (n = 5).  The microbial diversity of these samples was determined by pyrosequencing amplicons spanning the V3 hypervariable region of 16S rDNA region. 50 ng of V3 amplicons were sequenced on the 454-Genome Sequencer FLX sequencer by employing GS-FLX chemistry (454 Life Sciences) at the Advanced Genetic Analysis Center, University of Minnesota.  V3 Primers (5' to 3'): Forward: CCTACGGGAGGCAGCAG Reverse: ATTACCGCGGCTGCTGG  Multiplex barcode mapping is as follows: (#SampleID: BarcodeSequence) -- H1: CATTGCCATG ; H2: CTAAGCCTAG ; H3: TGAACGGCAT ; H4: TGAACAATCG ; H5: TGCCACCGAT ; C1: TACTGAGCTA ; C2: CGTGTCTCTA ;  C3: ATATCGCGAG ; C4: ACGCTCGACA ; C5: AGCACTGTAG