title: RNA-Seq of Anastrepha ludens: Family-10 strain: male pupa
design: RNA was extracted from a pool of male Anastrepha ludens in the pupal stage. Samples were collected from the Family-10 translocated genetic sexing strain reared in the mass-rearing facility in Petapa, Guatemala. Libraries were prepared using the Kapa mRNA stranded kit and multiplexed using NEB multiplex oligos.