title: GSM4565573: Untreated_OVCAR8_Day3; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was isolated using a QiAmp DNA blood extraction kit. Libraries were prepared as in: Wang T, Lander ES, & Sabatini DM (2016) Viral Packaging and Cell Culture for CRISPR-based Screens. Cold Spring Harb Protoc 2016(3): pdb.prot090811 PMID: 26933250, with the following exceptions: Forward PCR primer: AATGATACGGCGACCACCGAGATCTACACGAATACTGCCATTTGTCTCAAGATCTA  DNA Polymerase: ExTaq (Takara)  Genomic DNA/50 μL PCR reaction: 6 μg  Amplification cycles: 28
attributes.tag: GEO Accession
attributes.value: GSM4565573