title: GBS of Brachypodium distachyon Bd21XBd1-1: leaf tissue
design: Libraries were prepared as in Elshire et al., 2011 at the Wisconsin Madison Biotechnology Center with minimal modification. In short, 50ng of DNA was digested using the 5-bp cutter ApeKI after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase. 96 adapter ligated samples were pooled, amplified to provide sufficient DNA (2uM) for sequencing, and adapter dimers were removed by SPRI bead purfication. Quality and quantity of the finished linraries were assesed using Agilent Bioanalyzer high sensitivity Chip and Qubit dsDNA HS assay kit. Cluster generation was performed using HiSeq SR cluster kit v3 cBot kits. Flowcells were sequenced using single read, 100bp sequencing and Hiseq SBS king v3 on a HiSeq2000 sequencer.