title: Metagenome profiling in the sea adjacent to Chuuk, Federated States of Micronesiaon on March, 2014
design: Total DNA was extracted using the QIAeasy Plus Viral DNA/RNA Extraction Kit (Intron, Seongnam, Korea) according to the manufacturer’s instructions. The DNA library for pyrosequencing was prepared using the TruSeq Nano DNA library preparation kit according to the manufacturer’s manual (Illumina, San Diego, USA). The prepared DNA library was paired-end-sequenced using Illumina HiSeq 2000, which was performed by Macrogen, Seoul, Korea.