title: GSM3754712: CART19 cells, Nalm6 WT Stim rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted using Qiazol (Qiagen) from 300 CART19 cells and recovered by RNA Clean and Concentrator spin columns (Zymo) followed by incubation with Oligo-dT.  The transcription reaction was carried out on 100pg of cDNA for 1min at 55°C. 18 cycles of pre-amplification were performed followed by 14 cycles to add indexes/adaptors to prepare the libraries for sequencing.