title: GSM3018694: EMB-Ex-CD31+CD45+c-Kit+CD41-; Mus musculus; RNA-Seq
library_construction_protocol: Sorted YS-Ex-CD31+CD45+c-Kit+CD41- and Em-Ex-CD31+CD45+c-Kit+CD41- were resuspended in PBS containing 0.04%BSA at a concentration of 500-600 cells/ul. In total, 8600 cells per sample were loaded into each well of a 10X Chromium single cell capture chip with a recovery of 3000-3500 cells per sample. The captured cells then underwent lysis, reverse transcription, cDNA amplification, and library preparation with indexing per the manufacturer's protocol (10X Genomics). The libraries were sequenced together on an Illumina Nextseq500 using a high output 150 cycle kit with read lengths recommended by 10X Genomics.
attributes.tag: GEO Accession
attributes.value: GSM3018694