title: RAD-Seq in O. tshawytscha from Rock Creek - Umpqua River, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10NS0002 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AAACGG  OtsCORE-10NS0003 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 ACTCTT  OtsCORE-10NS0004 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 ATTCCG  OtsCORE-10NS0006 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CGAGGC  OtsCORE-10NS0008 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GAAATG  OtsCORE-10NS0009 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GGACTT  OtsCORE-10NS0010 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TAAGCT  OtsCORE-10NS0011 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TCTTCT  OtsCORE-10NS0016 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AACGTT  OtsCORE-10NS0020 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 ACTGGC  OtsCORE-10NS0021 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CAAAAA  OtsCORE-10NS0023 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CGCAGA  OtsCORE-10NS0027 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GAACCA  OtsCORE-10NS0029 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GGCAAG  OtsCORE-10NS0030 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TAATTC  OtsCORE-10NS0032 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TGAACC  OtsCORE-10NS0033 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AACTGA  OtsCORE-10NS0035 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AGCCAT  OtsCORE-10NS0037 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CAATCG  OtsCORE-10NS0039 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CGCGTG  OtsCORE-10NS0042 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GACGAC  OtsCORE-10NS0044 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GGGCGC  OtsCORE-10NS0046 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TACACA  OtsCORE-10NS0048 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TGACAA  OtsCORE-10NS0049 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AAGACG  OtsCORE-10NS0051 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AGCGCA  OtsCORE-10NS0056 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CACCTC  OtsCORE-10NS0057 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CGGTCC  OtsCORE-10NS0058 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GACTCT  OtsCORE-10NS0061 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GGGGCG  OtsCORE-10NS0062 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TACGGG  OtsCORE-10NS0063 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TGCCCG  OtsCORE-10NS0064 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AAGCTA  OtsCORE-10NS0065 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AGGGTC  OtsCORE-10NS0067 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CAGGCA  OtsCORE-10NS0070 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CGTCTA  OtsCORE-10NS0073 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GAGAGA  OtsCORE-10NS0074 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GGTACA  OtsCORE-10NS0077 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TAGTAT  OtsCORE-10NS0078 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TGCTTA  OtsCORE-10NS0079 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AATATC  OtsCORE-10NS0086 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 AGGTGT  OtsCORE-10NS0087 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CATACT  OtsCORE-10NS0088 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 CGTGAT  OtsCORE-10NS0091 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GATCGT  OtsCORE-10NS0092 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 GGTTTG  OtsCORE-10NS0093 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 TATCAC  OtsCORE-10NS0096 Rock Creek Hatchery. 425 McCarn Lane. Idleyld Park OR 97447 RL-0069 ATAGTA