title: PacBio WGS library from WI-38 cells
design: High molecular weight gDNA was extracted from WI-38 cells using Qiagens Gentra Puregene kit. High molecular weight gDNA was assessed for quality using pulsed-field gel electrophoresis. Genomic DNA was then sheared using needles, the Covaris g-Tube or the Megaruptor. 5 ug of sheared gDNA was then used as input into SMRTbell library construction (P/N 100-286-000-07). SMRTbell templates were size selected using Sage Sciences BluePippin. Sequencing was performed on PacBios RSII using the P6 polymerase with C4 chemistry.