title: GSM3753964: A_LV_S111 ATACseq; Homo sapiens; ATAC-seq
library_construction_protocol: Mononuclear cells from mesenteric LN, TDL, and PBMC were thawed, rested overnight, and sorted in cell numbers of 5-25,000 into complete RPMI with 50% FBS. Nuclei from cell pellets (5,000-25,000 cells) were isolated using a lysis buffer and pelleted in low-bind 1.5mL tubes (Eppendorf). Nuclei were resuspended in 25ml TD buffer with Tn transposase (Illumina). The transposition reaction was continued for 45 minutes at 37°C. The resulting DNA fragments were purified using the MinElute PCR purification Kit (Qiagen). Libraries were prepared using Nextera (Illumina) and sequenced by the Children's Hospital of Philadelphia partnership with the Bejing Genomics Institute on two lanes of a Hiseq 4000 paired-end sequencing (100 bp read length).