title: GSM1901476: islet_single_cell_22; Homo sapiens; RNA-Seq
library_construction_protocol: To disassociate islets into single cells, islets were incubated in Accutase (Life technology) in 37 °C for 20 min, neutralized by CMRL medium. Purification of single cells was performed by flow cytometry cell sorting on a Moflo AstriosEQ (Beckman Coulter, Miami) cDNA synthesis and enrichment was performed following the Smart-seq2 protocol as described (Picelli et al., 2014).
attributes.tag: GEO Accession
attributes.value: GSM1901476