title: GSM4189914: RS2_Fibro_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted following standard procedures based on Trizol and chloroform. The RNeasy Mini kit was used to purify RNA (Qiagen). Samples were incubated with DNAse I for 30 min to degrade genomic DNA. RNA was resuspended in RNAse-free water. The amount of RNA was quantified with Nanodrop. mRNA-Seq libraries were prepared using the TruSeq RNA Library Preparation Kit according to the manufacturer's instructions (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4189914