title: GSM2757483: Pv_BS_8wk_rep1; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated from each brain region at the indicated ages. WGBS libraries were constructed by using a similar method as previously described (Mo et al., 2015) except that NEXTflex methylated adapters (Bioo Scientific) were used. Briefly, DNA was sonicated to 200 bp using a Covaris S2. Bisulfite treatment was performed using EZ DNA Methylation-Gold kit (Zymo). Unmethylated lambda DNA (0.1-0.5%; Promega D1521) was spiked in to assess conversion rates.
attributes.tag: GEO Accession
attributes.value: GSM2757483