title: GSM2151023: e11.5_forelimb_distal_ko_mRNA_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Micro-dissected E11.5 forelimb ZAPs from wild type and mutant embryos were stored at -80 C in Qiagen RNA later solution until genotyped. For each genotype, RNA was extracted from pools of micro-dissected tissue samples (N=4) with Qiagen Rneasy micro kit following manufacturer's protocol. The quality of total RNA from two independent pools of limb tissue for each genotype was analyzed on RNA 6000 Pico kit (Agilent 2100 bioanalyzer), followed by ribosomal RNA depletion using Ribo-Zero Magnetic Gold Kit (Epicentre). Strand-specific RNA-Seq libraries were produced using Illumina TruSeq Stranded Total RNA - low sample protocol. The resulting cDNA was subjected to 8 cycles of PCR amplification and sequenced on a HiSeq 2000 instrument with a paired-end 50 cycles protocol.
attributes.tag: GEO Accession
attributes.value: GSM2151023