title: RNA-seq of mus musculus germ line E11.5 control Rep2
design: Germ line cells (Oct4EGFP-positive) were isolated by FACS from control and Dazl-deficient embryonic XX (female) mice at E10.2 (1-3 tail somites) and E11.5 (17-18 tail somites), n = three embryos at each time point.  RNA was extracted using Quick-RNA MiniPrep kit (Zymo Research, Irvine CA).  Reverse Transcription was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View CA), and sequencing performed using a HiSeq2000 (Illumina).