title: RAD-Seq in O. tshawytscha from Johnson Creek, ID & Yakima River, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  BonnCH042711-3.02 Yakima River RL-0087 ACATAC  Bonn-CH-042908-3.04 Yakima River RL-0087 TCCTGC  BonnCH042911-5.06 Yakima River RL-0087 TTCTAG  Bonn-CH-043008-6.02 Yakima River RL-0087 TTCCGT  BonnCH050211-3.05 Yakima River RL-0087 CCGAGG  Bonn-CH-050409-1.08 Yakima River RL-0087 GCAGAT  BonnCH050411-4.02 Yakima River RL-0087 ATCAAA  BonnCH050511-6.05 Yakima River RL-0087 TTGAGC  BonnCH050511-6.06 Yakima River RL-0087 CTGGTT  Bonn-CH-050608-1.07 Yakima River RL-0087 ACAGCG  Bonn-CH-050608-5.08 Yakima River RL-0087 GTCATC  Bonn-CH-050609-1.01 Yakima River RL-0087 GTAAGT  BonnCH050611-6.08 Yakima River RL-0087 GCGCTG  Bonn-CH-050808-1.04 Yakima River RL-0087 ATAGTA  Bonn-CH-050808-1.09 Yakima River RL-0087 CCCTAA  Bonn-CH-050809-2.01 Yakima River RL-0087 AGTAGG  BonnCH051011-1.06 Yakima River RL-0087 GCGACC  BonnCH051011-1.09 Yakima River RL-0087 GTGCCT  BonnCH051111-4.04 Yakima River RL-0087 TCGCCA  BonnCH051111-5.03 Yakima River RL-0087 ATGCAC  Bonn-CH-051208-1.04 Yakima River RL-0087 CTGCGA  Bonn-CH-051208-2.03 Yakima River RL-0087 GCCGTA  Bonn-CH-051308-4.07 Yakima River RL-0087 AGTTAA  Bonn-CH-051308-6.08 Yakima River RL-0087 CCCGGT  BonnCH051311-1.01 Yakima River RL-0087 CCGCAT  BonnCH051311-1.02 Yakima River RL-0087 GTGTAA  BonnCH051311-3.02 Yakima River RL-0087 ACCATG  BonnCH051311-5.05 Yakima River RL-0087 CTTATG  Bonn-CH-051408-2.03 Yakima River RL-0087 CTCGCC  Bonn-CH-051409-1.08 Yakima River RL-0087 AATGAG  Bonn-CH-051508-1.01 Yakima River RL-0087 GCATGG  Bonn-CH-051508-1.02 Yakima River RL-0087 GTATCC  Bonn-CH-051809-2.07 Yakima River RL-0087 ACAAGA  Bonn-CH-051909-1.07 Yakima River RL-0087 CTACAG  Bonn-CH-052109-1.07 Yakima River RL-0087 TTATGA  Bonn-CH-052109-5.08 Yakima River RL-0087 TCAAAG  BonnCH052311-4.02 Yakima River RL-0087 TCGGAC  Bonn-CH-052608-3.08 Yakima River RL-0087 TCGATT  BonnCH052611-1.01 Yakima River RL-0087 ATGTTG  BonnCH052711-3.09 Yakima River RL-0087 TTTAAT  BonnCH053011-2.05 Yakima River RL-0087 TCTCGG  BonnCH053111-1.03 Yakima River RL-0087 ACCCCC  BonnCH053111-2.05 Yakima River RL-0087 CCTAAC  BonnCH060611-1.03 Yakima River RL-0087 GCTCAA  BonnCH060611-1.09 Yakima River RL-0087 GTTGGA  BonnCH060711-2.08 Yakima River RL-0087 CTTTGC  BonnCH061511-1.09 Yakima River RL-0087 TTTGTC  Bonn-CH-062209-3.01 Yakima River RL-0087 CCATTT  OtsJCP11-0001 JOHNSON CREEK WEIR RL-0087 AAACGG  OtsJCP11-0002 JOHNSON CREEK WEIR RL-0087 ACTCTT  OtsJCP11-0003 JOHNSON CREEK WEIR RL-0087 ATTCCG  OtsJCP11-0004 JOHNSON CREEK WEIR RL-0087 CGAGGC  OtsJCP11-0006 JOHNSON CREEK WEIR RL-0087 GAAATG  OtsJCP11-0007 JOHNSON CREEK WEIR RL-0087 GGACTT  OtsJCP11-0008 JOHNSON CREEK WEIR RL-0087 TAAGCT  OtsJCP11-0009 JOHNSON CREEK WEIR RL-0087 TCTTCT  OtsJCP11-0011 JOHNSON CREEK WEIR RL-0087 AACGTT  OtsJCP11-0012 JOHNSON CREEK WEIR RL-0087 ACTGGC  OtsJCP11-0013 JOHNSON CREEK WEIR RL-0087 CAAAAA  OtsJCP11-0014 JOHNSON CREEK WEIR RL-0087 CGCAGA  OtsJCP11-0016 JOHNSON CREEK WEIR RL-0087 GAACCA  OtsJCP11-0019 JOHNSON CREEK WEIR RL-0087 GGCAAG  OtsJCP11-0020 JOHNSON CREEK WEIR RL-0087 TAATTC  OtsJCP11-0021 JOHNSON CREEK WEIR RL-0087 TGAACC  OtsJCP11-0022 JOHNSON CREEK WEIR RL-0087 AACTGA  OtsJCP11-0023 JOHNSON CREEK WEIR RL-0087 AGCCAT  OtsJCP11-0024 JOHNSON CREEK WEIR RL-0087 CAATCG  OtsJCP11-0032 JOHNSON CREEK WEIR RL-0087 CGCGTG  OtsJCP11-3080 JOHNSON CREEK WEIR RL-0087 GACGAC  OtsJCP11-3081 JOHNSON CREEK WEIR RL-0087 GGGCGC  OtsJCP11-3082 JOHNSON CREEK WEIR RL-0087 TACACA  OtsJCP11-3435 JOHNSON CREEK WEIR RL-0087 TGACAA  OtsJCP11-3438 JOHNSON CREEK WEIR RL-0087 AAGACG  OtsJCP11-3444 JOHNSON CREEK WEIR RL-0087 AGCGCA  OtsJCP11-3445 JOHNSON CREEK WEIR RL-0087 CACCTC  OtsJCP11-3446 JOHNSON CREEK WEIR RL-0087 CGGTCC  OtsJCP11-3452 JOHNSON CREEK WEIR RL-0087 GGGGCG  OtsJCP11-3454 JOHNSON CREEK WEIR RL-0087 TACGGG  OtsJCP11-3455 JOHNSON CREEK WEIR RL-0087 TGCCCG  OtsJCP11-3456 JOHNSON CREEK WEIR RL-0087 AAGCTA  OtsJCP11-3464 JOHNSON CREEK WEIR RL-0087 AGGGTC  OtsJCP11-3465 JOHNSON CREEK WEIR RL-0087 CAGGCA  OtsJCP11-3466 JOHNSON CREEK WEIR RL-0087 CGTCTA  OtsJCP11-3469 JOHNSON CREEK WEIR RL-0087 GAGAGA  OtsJCP11-3471 JOHNSON CREEK WEIR RL-0087 GGTACA  OtsJCP11-3481 JOHNSON CREEK WEIR RL-0087 TAGTAT  OtsJCP11-3482 JOHNSON CREEK WEIR RL-0087 TGCTTA  OtsJCP11-3488 JOHNSON CREEK WEIR RL-0087 AATATC  OtsJCP11-3502 JOHNSON CREEK WEIR RL-0087 AGGTGT  OtsJCP11-3563 JOHNSON CREEK WEIR RL-0087 CATACT  OtsJCP11-3564 JOHNSON CREEK WEIR RL-0087 CGTGAT  OtsJCP11-3567 JOHNSON CREEK WEIR RL-0087 GATCGT  OtsJCP11-3571 JOHNSON CREEK WEIR RL-0087 GGTTTG  OtsJCP11-3572 JOHNSON CREEK WEIR RL-0087 TATCAC  OtsJCP11-3580 JOHNSON CREEK WEIR RL-0087 TGGGGA