title: GSM4565883: DC3_TLR_1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were FACS sorted in in 1.5ml Eppendorf tubes (100-1000 cells/tube) containing Trizol RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked with Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA). RNA library preparations, sequencing reactions, and initial bioinformatics analysis were conducted at GENEWIZ, LLC. (South Plainfield, NJ, USA). The SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA) as per manufacturer's protocol. Illumina Nextera XT library was used for library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed by limited-cycle PCR to enrich and add index to the cDNA fragments. Final libraries were analyzed on the Agilent TapeStation for library sizing and quantified using the Qubit dsDNA HS Assay Kit and by qPCR using the KAPA Library Quantification Kit. The sequencing libraries were multiplexed and clustered on four lanes of a flowcell. After clustering, the flowcell was loaded on the Illumina HiSeq instrument according to manufacturer's instructions. The samples were sequenced using a 2x150 Paired End (PE) configuration.
attributes.tag: GEO Accession
attributes.value: GSM4565883