title: RAD-Seq in O. tshawytscha from Robertson Creek Hatchery, British Columbia
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10NO0001 Robertson Creek Hatchery - British Columbia RL-0054 AAACGG  OtsCORE-10NO0002 Robertson Creek Hatchery - British Columbia RL-0054 ACTCTT  OtsCORE-10NO0003 Robertson Creek Hatchery - British Columbia RL-0054 ATTCCG  OtsCORE-10NO0004 Robertson Creek Hatchery - British Columbia RL-0054 CGAGGC  OtsCORE-10NO0005 Robertson Creek Hatchery - British Columbia RL-0054 GAAATG  OtsCORE-10NO0006 Robertson Creek Hatchery - British Columbia RL-0054 GGACTT  OtsCORE-10NO0007 Robertson Creek Hatchery - British Columbia RL-0054 TAAGCT  OtsCORE-10NO0008 Robertson Creek Hatchery - British Columbia RL-0054 TCTTCT  OtsCORE-10NO0009 Robertson Creek Hatchery - British Columbia RL-0054 AACGTT  OtsCORE-10NO0010 Robertson Creek Hatchery - British Columbia RL-0054 ACTGGC  OtsCORE-10NO0011 Robertson Creek Hatchery - British Columbia RL-0054 CAAAAA  OtsCORE-10NO0012 Robertson Creek Hatchery - British Columbia RL-0054 CGCAGA  OtsCORE-10NO0013 Robertson Creek Hatchery - British Columbia RL-0054 GAACCA  OtsCORE-10NO0014 Robertson Creek Hatchery - British Columbia RL-0054 GGCAAG  OtsCORE-10NO0015 Robertson Creek Hatchery - British Columbia RL-0054 TAATTC  OtsCORE-10NO0016 Robertson Creek Hatchery - British Columbia RL-0054 TGAACC  OtsCORE-10NO0017 Robertson Creek Hatchery - British Columbia RL-0054 AACTGA  OtsCORE-10NO0018 Robertson Creek Hatchery - British Columbia RL-0054 AGCCAT  OtsCORE-10NO0019 Robertson Creek Hatchery - British Columbia RL-0054 CAATCG  OtsCORE-10NO0020 Robertson Creek Hatchery - British Columbia RL-0054 CGCGTG  OtsCORE-10NO0021 Robertson Creek Hatchery - British Columbia RL-0054 GACGAC  OtsCORE-10NO0022 Robertson Creek Hatchery - British Columbia RL-0054 GGGCGC  OtsCORE-10NO0023 Robertson Creek Hatchery - British Columbia RL-0054 TACACA  OtsCORE-10NO0024 Robertson Creek Hatchery - British Columbia RL-0054 TGACAA  OtsCORE-10NO0025 Robertson Creek Hatchery - British Columbia RL-0054 AAGACG  OtsCORE-10NO0026 Robertson Creek Hatchery - British Columbia RL-0054 AGCGCA  OtsCORE-10NO0027 Robertson Creek Hatchery - British Columbia RL-0054 CACCTC  OtsCORE-10NO0028 Robertson Creek Hatchery - British Columbia RL-0054 CGGTCC  OtsCORE-10NO0029 Robertson Creek Hatchery - British Columbia RL-0054 GACTCT  OtsCORE-10NO0030 Robertson Creek Hatchery - British Columbia RL-0054 GGGGCG  OtsCORE-10NO0031 Robertson Creek Hatchery - British Columbia RL-0054 TACGGG  OtsCORE-10NO0032 Robertson Creek Hatchery - British Columbia RL-0054 TGCCCG  OtsCORE-10NO0033 Robertson Creek Hatchery - British Columbia RL-0054 AAGCTA  OtsCORE-10NO0034 Robertson Creek Hatchery - British Columbia RL-0054 AGGGTC  OtsCORE-10NO0035 Robertson Creek Hatchery - British Columbia RL-0054 CAGGCA  OtsCORE-10NO0036 Robertson Creek Hatchery - British Columbia RL-0054 CGTCTA  OtsCORE-10NO0037 Robertson Creek Hatchery - British Columbia RL-0054 GAGAGA  OtsCORE-10NO0038 Robertson Creek Hatchery - British Columbia RL-0054 GGTACA  OtsCORE-10NO0039 Robertson Creek Hatchery - British Columbia RL-0054 TAGTAT  OtsCORE-10NO0040 Robertson Creek Hatchery - British Columbia RL-0054 TGCTTA  OtsCORE-10NO0041 Robertson Creek Hatchery - British Columbia RL-0054 AATATC  OtsCORE-10NO0042 Robertson Creek Hatchery - British Columbia RL-0054 AGGTGT  OtsCORE-10NO0043 Robertson Creek Hatchery - British Columbia RL-0054 CATACT  OtsCORE-10NO0044 Robertson Creek Hatchery - British Columbia RL-0054 CGTGAT  OtsCORE-10NO0045 Robertson Creek Hatchery - British Columbia RL-0054 GATCGT  OtsCORE-10NO0046 Robertson Creek Hatchery - British Columbia RL-0054 GGTTTG  OtsCORE-10NO0047 Robertson Creek Hatchery - British Columbia RL-0054 TATCAC  OtsCORE-10NO0048 Robertson Creek Hatchery - British Columbia RL-0054 GTAAGT