title: 16S rRNA of feces: male mice
design: Frozen fecal samples (200  20 mg) were used for genomic DNA extraction in accordance with the protocol provided by the QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA), followed by PCR amplification of theV3 region of the 16S rRNA gene and sequencing using the Illumina MiSeq system (Illumina, USA). The paired-end sequences generated were quality filtered and then clustered into operational taxonomic units (OTUs) with FASTX Toolkit 0.0.13