title: GSM3752611: cpsf30-3 rep2 (TL7); Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA were extracted from 9 day-old seedlings for WT, fip37L, cpsf30-3 and cpsf30-1 mutant plants using the RNeasy Plant Mini Kit and treated onto the column with the RNase-Free DNase Set. Two replicates were performed per line. PolyA purification, library preparation using the Illumina TruSeq stranded mRNA kit and library quality controls were performed by Fasteris SA. RNA libraries were prepared for sequencing bylocal BioEnvioenment NGS platform using standard Illumina protocols. Sequencing was performed using Illumina procedure with single-end reads ,1x 75bp and 35-50x106 reads per library