title: GSM3755008: BMNC_Subj6; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were isolated and RNA purified with TriReagent followed by oligo dT selection. RNA was submitted to BGI Europa and analyzed according to their standard procedure: mRNA enriched by oligo dT selection was fragmented and reverse transcribed by N6 random priming. The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments and the ligation products purified and subjected to PCR amplification. The PCR products were denatured by heat and the single strand DNA cyclized by splint oligo and DNA ligase. Finally, they were sequenced on the BGISEQ-500 platform
attributes.tag: GEO Accession
attributes.value: GSM3755008