title: RAD-seq of single_bulk: leaves
design: Genomic DNA was isolated from freshly harvested leaves (about 0.02 g) of the P1, P2, F1, and F2 plants from the 2014 experiment, using the Plant Genomic DNA Kit (TIANGEN Biotech, Beijing, China) following the manufacturerfs instructions. For QTL-seq [6], two DNA bulks were prepared. The single branch (SB) bulk was prepared by mixing equal amounts of DNA from 60 single branches (the median of BN was 1 and >70% of the inflorescences were single branch type within the plant) and the highly-branched bulk (HB) was constructed by mixing equal amounts of DNA from 11 highly branched (BN > 16) inflorescences of F2 plants from the 2014 experiment.