title: RNA-seq of early instar  Drepana arcuata
design: RNA was extracted from whole  bodies of group of early instars (Instars I,II; N=20; flash frozen using  liquid nitrogen) using Norgen Biotek RNA extraction kit (Norgen Biotek Corp,  Thorold, ON, Canada). Larvae from single female were used in this study, for  all the biological replicates. Agilent Bioanalyser (Santa Clara, CA, United  States) was used to assess the quality and quantity of RNA samples. Library  preparation and 150bp paired end sequencing was carried out using TruSeq RNA  v2 Library Prep Kit and Illumina NextSeq500, respectively at the StemCore  sequencing facility (Ottawa Hospital Research Institute, ON, Canada).