title: RAD-Seq in O. tshawytscha from Cowlitz River, WA & Wenatchee River, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  04FJ-09 Cowlitz River Hatchery RL-0044 GGACTT  04FJ-100 Cowlitz River Hatchery RL-0044 TGAACC  04FJ-103 Cowlitz River Hatchery RL-0044 CACCTC  04FJ-106 Cowlitz River Hatchery RL-0044 AAGACG  04FJ-108 Cowlitz River Hatchery RL-0044 CGCAGA  04FJ-111 Cowlitz River Hatchery RL-0044 TACGGG  04FJ-112 Cowlitz River Hatchery RL-0044 CAGGCA  04FJ-115 Cowlitz River Hatchery RL-0044 TAGTAT  04FJ-117 Cowlitz River Hatchery RL-0044 GACTCT  04FJ-119 Cowlitz River Hatchery RL-0044 CAATCG  04FJ-121 Cowlitz River Hatchery RL-0044 TACACA  04FJ-122 Cowlitz River Hatchery RL-0044 ACTCTT  04FJ-123 Cowlitz River Hatchery RL-0044 GAGAGA  04FJ-125 Cowlitz River Hatchery RL-0044 CGGTCC  04FJ-127 Cowlitz River Hatchery RL-0044 AGGTGT  04FJ-129 Cowlitz River Hatchery RL-0044 GGCAAG  04FJ-13 Cowlitz River Hatchery RL-0044 TGCCCG  04FJ-130 Cowlitz River Hatchery RL-0044 GGGGCG  04FJ-131 Cowlitz River Hatchery RL-0044 GGTACA  04FJ-138 Cowlitz River Hatchery RL-0044 GAAATG  04FJ-139 Cowlitz River Hatchery RL-0044 CGTCTA  04FJ-141 Cowlitz River Hatchery RL-0044 AGCCAT  04FJ-142 Cowlitz River Hatchery RL-0044 AGGGTC  04FJ-143 Cowlitz River Hatchery RL-0044 ACTGGC  04FJ-144 Cowlitz River Hatchery RL-0044 TAATTC  04FJ-145 Cowlitz River Hatchery RL-0044 CATACT  04FJ-147 Cowlitz River Hatchery RL-0044 ATTCCG  04FJ-148 Cowlitz River Hatchery RL-0044 AGCGCA  04FJ-149 Cowlitz River Hatchery RL-0044 AATATC  04FJ-22 Cowlitz River Hatchery RL-0044 CGTGAT  04FJ-32 Cowlitz River Hatchery RL-0044 TGCTTA  04FJ-48 Cowlitz River Hatchery RL-0044 GGGCGC  04FJ-53 Cowlitz River Hatchery RL-0044 CGCGTG  04FJ-56 Cowlitz River Hatchery RL-0044 CAAAAA  04FJ-66 Cowlitz River Hatchery RL-0044 AACTGA  04FJ-67 Cowlitz River Hatchery RL-0044 TCTTCT  04FJ-70 Cowlitz River Hatchery RL-0044 GACGAC  04FJ-72 Cowlitz River Hatchery RL-0044 GATCGT  04FJ-74 Cowlitz River Hatchery RL-0044 CGAGGC  04FJ-76 Cowlitz River Hatchery RL-0044 GGTTTG  04FJ-77 Cowlitz River Hatchery RL-0044 GAACCA  04FJ-84 Cowlitz River Hatchery RL-0044 TGACAA  04FJ-85 Cowlitz River Hatchery RL-0044 AACGTT  04FJ-88 Cowlitz River Hatchery RL-0044 TATCAC  04FJ-90 Cowlitz River Hatchery RL-0044 TAAGCT  04FJ-92 Cowlitz River Hatchery RL-0044 TGGGGA  04FJ-97 Cowlitz River Hatchery RL-0044 AAGCTA  93DD-01 Wenatchee RL-0044 AGTTAA  93DD-02 Wenatchee RL-0044 ACCATG  93DD-06 Wenatchee RL-0044 ACAGCG  93DD-12 Wenatchee RL-0044 CCCTAA  93DD-14 Wenatchee RL-0044 CTGCGA  93DD-15 Wenatchee RL-0044 CTCGCC  93DD-16 Wenatchee RL-0044 TCGGAC  93DD-17 Wenatchee RL-0044 GTATCC  93DD-21 Wenatchee RL-0044 GCGCTG  93DD-23 Wenatchee RL-0044 GTGTAA  93DD-26 Wenatchee RL-0044 ACAAGA  93DD-27 Wenatchee RL-0044 GCATGG  93DD-30 Wenatchee RL-0044 CCATTT  93DD-31 Wenatchee RL-0044 TTGAGC  93DD-35 Wenatchee RL-0044 CTGGTT  93DD-36 Wenatchee RL-0044 AATGAG  93DD-37 Wenatchee RL-0044 ATGCAC  93DD-39 Wenatchee RL-0044 ATCAAA  93DD-42 Wenatchee RL-0044 CTTTGC  93DD-43 Wenatchee RL-0044 GTGCCT  93DD-44 Wenatchee RL-0044 GTTGGA  93DD-47 Wenatchee RL-0044 TCCTGC  93DE-06 Wenatchee RL-0044 GCAGAT  93DE-09 Wenatchee RL-0044 CTACAG  93DE-32 Wenatchee RL-0044 CCCGGT  93DE-36 Wenatchee RL-0044 GCCGTA  93DE-43 Wenatchee RL-0044 TCGCCA  93DE-48 Wenatchee RL-0044 CTTATG  93DE-52 Wenatchee RL-0044 CCGCAT  93DE-55 Wenatchee RL-0044 ACATAC  93DE-58 Wenatchee RL-0044 TTCCGT  93DE-61 Wenatchee RL-0044 CCTAAC  93DE-65 Wenatchee RL-0044 AGTAGG  93DE-72 Wenatchee RL-0044 GCGACC  93DE-75 Wenatchee RL-0044 CCGAGG  93DE-77 Wenatchee RL-0044 TCGATT  93DE-83 Wenatchee RL-0044 TTATGA  93DE-84 Wenatchee RL-0044 TTTGTC  93DE-85 Wenatchee RL-0044 ATAGTA  93DE-86 Wenatchee RL-0044 GCTCAA  93DE-87 Wenatchee RL-0044 TTCTAG  93DE-88 Wenatchee RL-0044 ACCCCC  93DE-89 Wenatchee RL-0044 TTTAAT  93DE-93 Wenatchee RL-0044 TCTCGG  93DE-94 Wenatchee RL-0044 GTAAGT  93DE-95 Wenatchee RL-0044 ATGTTG  93DE-96 Wenatchee RL-0044 GTCATC  93DE-99 Wenatchee RL-0044 TCAAAG