title: GSM2149077: RNASeq ABP1d 2; Schizosaccharomyces pombe; ncRNA-Seq
library_construction_protocol: total RNA extraction by acid phenol protocol total RNA was quantified by Qubit fluorometer using the Quant-iT RNA Assay Kit (Life Technologies). Using Illumina’s small RNA Sample Prep Kit v1.5, 3’ and 5’ adapters were ligated to 5 µg of total RNA, followed by reverse transcription and 12 cycles of amplification. Amplification products of 125 to 250 nucleotides size selected from a 4% agarose gel and purified. Libraries were quantified by Qubit fluorometer using the Quant-iT DNA HS Assay Kit (Life Technologies). Using Illumina’s Cluster Generation Kit, libraries were subject to cluster generation at 8 pM concentration as one sample per lane. Sequencing-by-synthesis was performed for 38 cycles. Strand-specific RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM2149077