title: GSM2417073: 48h_KpMX_ATAC-seq; Mus musculus; ATAC-seq
library_construction_protocol: genomic DNA 50000 cells (129v MEFs, un-induced MEFs, 48hOSKM, 48hOct4, 48hSox2, 48hKlf4, pre-i#1, pre-i#2 or ESCs) were re-suspended in 50μl lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, and 1 × Complete Protease inhibitor (Roche)) and spun at 500g for 10 min at 4 °C to collect nuclei. Nuclei were washed in 1x PBS and subsequently re-suspended in 50 μl Transposase reaction (25 μl 2 × Tagmentation buffer, 22.5 μl water, 2.5 μl Tn5 Transposase enzyme, following instructions by Illumina). Reactions were incubated for 30 min at 37 °C and DNA purified using Qiagen MinElute columns (Qiagen). The transposed DNA was subsequently amplified with custom primers as described (Buenrostro et al., 2013) for 7-9 cycles and libraries were visualized on a 2% TBE gel prior to sequencing with a single-end-sequencing length of 50 nucleotides
attributes.tag: GEO Accession
attributes.value: GSM2417073