title: GSM4677826: GM-RNA-IP; Mus musculus; OTHER
library_construction_protocol: RNA was isolated using an Omega E.Z.N.A.® Total RNA Kit I. mRNA was isolated using Arraystar Seq-StarTM poly(A) mRNA Isolation Kit . Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The KAPA Stranded mRNA-seq Kit (Illumina, San Diego, CA, USA) was used to prepare the RNA-seq libraries for m6A immunoprecipitated mRNA and input control mRNA libraries. Quality of the libraries was determined using an Agilent 2100 Bioanalyzer. The HiSeq 3000/4000 PE Cluster Kit (Illumina, San Diego, CA, USA) was used to generate clusters from the prepared libraries following the manufacturer's instructions. The clustered libraries were sequenced on an Illumina HiSeq 4000 system. MeRIP-seq or RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM4677826