title: GSM3171679: H3K27Ac_ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin was fragmaneted with sonicaiton and precleared prior to overnight incubation with specific antibodies Library was prepared using NEBNext® Ultra DNA Library Prep Kit and NEBNext® Multiplex Oligos for Illumina (NEB) according to the manufacturer's recommendations DNA extraction and chromatin immunoprecipitation were performed  as previously described in Galli et al., 2012 doi 10.1016/j.molcel.2015.09.001 Libraries for ChIP-sequencing were generated by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and barcoding added using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB) according to the manufacturer's recommendation.
attributes.tag: GEO Accession
attributes.value: GSM3171679