title: GSM1629375: ChIP-Seq CREB minus Reelin; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIP-seq, samples were fixed for 45 minutes with 2mM DSG followed by 10 minutes with 1% formaldehyde. Fixation was stopped by adding Glycine (0.125M). Chromatin DNA was sheared to 200–500 bp average in size by sonication. Soluble sheared chromatin was immunoprecipitated with specific antibodies coupled with protein G dynabeads (Invitrogen) overnight at 4°C. After washing and elution, the protein–DNA complex was decross-linked by heating at 65°C for 4 hours. Immunoprecipitated DNA was purified by using iPure Diagenode kit. the libraries were constructed following Illumina’s Chip-Seq Sample prep kit.
attributes.tag: GEO Accession
attributes.value: GSM1629375