title: RAD-Seq in O. tshawytscha from Imnaha River, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsIMNW11S_0001 Imnaha River RL-0070 AATGAG  OtsIMNW11S_0010 Imnaha River RL-0070 AGTAGG  OtsIMNW11S_0012 Imnaha River RL-0070 CCATTT  OtsIMNW11S_0013 Imnaha River RL-0070 CTACAG  OtsIMNW11S_0015 Imnaha River RL-0070 GCAGAT  OtsIMNW11S_0031 Imnaha River RL-0070 GTAAGT  OtsIMNW11S_0038 Imnaha River RL-0070 TCAAAG  OtsIMNW11S_0044 Imnaha River RL-0070 TTATGA  OtsIMNW11S_0051 Imnaha River RL-0070 ACAAGA  OtsIMNW11S_0053 Imnaha River RL-0070 AGTTAA  OtsIMNW11S_0055 Imnaha River RL-0070 CCCGGT  OtsIMNW11S_0057 Imnaha River RL-0070 CTCGCC  OtsIMNW11S_0059 Imnaha River RL-0070 GCATGG  OtsIMNW11S_0071 Imnaha River RL-0070 GTATCC  OtsIMNW11S_0074 Imnaha River RL-0070 TCCTGC  OtsIMNW11S_0079 Imnaha River RL-0070 TTCCGT  OtsIMNW11S_0086 Imnaha River RL-0070 ACAGCG  OtsIMNW11S_0088 Imnaha River RL-0070 ATAGTA  OtsIMNW11S_0090 Imnaha River RL-0070 CCCTAA  OtsIMNW11S_0094 Imnaha River RL-0070 CTGCGA  OtsIMNW11S_0096 Imnaha River RL-0070 GCCGTA  OtsIMNW11S_0120 Imnaha River RL-0070 GTCATC  OtsIMNW11S_0121 Imnaha River RL-0070 TCGATT  OtsIMNW11S_0139 Imnaha River RL-0070 TTCTAG  OtsIMNW11S_0141 Imnaha River RL-0070 ACATAC  OtsIMNW11S_0143 Imnaha River RL-0070 ATCAAA  OtsIMNW11S_0145 Imnaha River RL-0070 CCGAGG  OtsIMNW11S_0148 Imnaha River RL-0070 CTGGTT  OtsIMNW11S_0150 Imnaha River RL-0070 GCGACC  OtsIMNW11S_0151 Imnaha River RL-0070 GTGCCT  OtsIMNW11S_0191 Imnaha River RL-0070 TCGCCA  OtsIMNW11S_0205 Imnaha River RL-0070 TTGAGC  OtsIMNW11S_0206 Imnaha River RL-0070 ACCATG  OtsIMNW11S_0207 Imnaha River RL-0070 ATGCAC  OtsIMNW11S_0209 Imnaha River RL-0070 CCGCAT  OtsIMNW11S_0219 Imnaha River RL-0070 CTTATG  OtsIMNW11S_0232 Imnaha River RL-0070 GCGCTG  OtsIMNW11S_0234 Imnaha River RL-0070 GTGTAA  OtsIMNW11S_0235 Imnaha River RL-0070 TCGGAC  OtsIMNW11S_0237 Imnaha River RL-0070 TTTAAT  OtsIMNW11S_0239 Imnaha River RL-0070 ACCCCC  OtsIMNW11S_0241 Imnaha River RL-0070 ATGTTG  OtsIMNW11S_0243 Imnaha River RL-0070 CCTAAC  OtsIMNW11S_0245 Imnaha River RL-0070 CTTTGC  OtsIMNW11S_0247 Imnaha River RL-0070 GCTCAA  OtsIMNW11S_0249 Imnaha River RL-0070 GTTGGA  OtsIMNW11S_0250 Imnaha River RL-0070 TCTCGG  OtsIMNW11S_0251 Imnaha River RL-0070 TACACA