title: "soil sample G2729, PCR primers: fwd-gITS7, rev-ITS4": Sample G2729_2
design: "40 pooled samples (50 mm diam to 5 cm depth) from 2500-m2 plot; 2 g DNA extracted using MoBio Soil Maxi kit; PCR targeting ITS region of fungi using MID-tagged primers (fwd: GTGARTCATCGARTCTTTG, rev: MID-TCCTSCGCTTATTGATATGC); SequalPrep Normalisation; adapter ligation; emPCR"