title: NA-Seq of Persimmon peel: Softened
design: Libraries for RNA-seq were prepared using an Illumina TruSeq DNA/RNA library preparation kit according to the manufacturer's protocol. The cDNA library was constructed as described in the Illumina protocol. Briefly, RNA was fragmented to an average length of 200-300 bp by Mg2+-catalyzed hydrolysis and was then reverse transcribed to cDNA with random priming. cDNA was then subjected to end repair, adaptor ligation, size selection, and PCR amplification. Each cDNA library was 101 bp paired-end sequenced using an Illumina Hiseq2000 sequencer according to the manufacturer's protocols.