title: "soil sample G2675, PCR primers: fwd-ITS1F, rev-ITS2": Sample G2675_2
design: "40 pooled samples (50 mm diam to 5 cm depth) from 2500-m2 plot; 2 g DNA extracted using MoBio Soil Maxi kit; PCR targeting ITS region of fungi using MID-tagged primers (fwd: MID-GGTCATTTAGAGGAAGTAA, rev: GCTGCGTTCTTCATCGATGC); SequalPrep Normalisation; adapter ligation; emPCR"