title: unspecified sequencing; qiime_experiment_2202:1247466:104
design: daily microbiota time-series
library_construction_protocol: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?_1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: experiment_title
attributes.value: MIT daily human microbiota time-series
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: sample_center
attributes.value: MIT
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: run_date
attributes.value: 5/14/11
attributes.tag: experiment_center
attributes.value: CCME
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: run_center
attributes.value: CGS-GL
attributes.tag: num_sequences
attributes.value: 189377
attributes.tag: platform
attributes.value: Illumina
attributes.tag: library_construction_protocol
attributes.value: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?_1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: experiment_design_description
attributes.value: daily microbiota time-series
attributes.tag: row_number
attributes.value: 104
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: study_center
attributes.value: MIT