title: GSM1528100: bFGF_2; Mus musculus; RNA-Seq
library_construction_protocol: Inner ears were removed and trypsinized to dissociate cells.  Cells that form otospheres were harvested and RNA obtained from these cells using Trizol reagent. RNA-Seq was done using protocol from Marioni et al. 2008.  RNA-Seq from otospheres were done using Ovation RNA-Seq System v2 kit (NuGEN).  1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM1528100