title: Targeted sequencing of human: blood DNA
design: Genomic DNA was isolated from human peripheral blood lymphocytes by                 the standard phenol/chloroform extraction and ethanol precipitation method. Target                 enrichment was performed by the Nimblegens SeqCap EZ Choice (Roche) using a standard                 SeqCap protocol. 500 ng of DNA was fragmented to an average size 400 bp using                 BioRuptor or Covaris M220 instruments and libraries were prepared using the Rapid                 Library Preparation Kit (Roche). Sff files were demultiplexed using sfffile program                 from the 454 software.