title: 16S V3V4 rRNA amplicon of Gulf of Mexico marine sediment
design: Total DNA was extracted and purified from 0.5 g of sediment using the PowerSoil DNA isolation kit (MO BIO-QIAGEN). The DNA was used to amplify V3-V4 16S rRNA variable regions using the primers pair S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 (Klindworth et al., 2013). Amplicon libraries were sequenced in the Illumina MiSeq platform with a paired-end reads configuration of 600 cycles