title: Illumina HiSeq 4000 sequencing; 3prime RNA-seq of murine SCLC cell line modified with CRISPR-activation of Myc, Mycl or Mycn
design: 3prime RNA-seq of murine SCLC cell line modified with CRISPR-activation of Myc, Mycl or Mycn
library_construction_protocol: Cells pellets were harvested under steady state growth conditions. Total RNA was extracted using the Qiagen RNeasy Mini kit. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)
attributes.tag: Experimental Factor: genotype
attributes.value: lentiSAMv; Mycn sgRNA