title: GSM3754033: CI-AKI Rep2_mRNA; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted with mirVana miRNA Isolation Kit (Cat #. AM1561, Austin TX, US) and its Standard Operating Protocol(SOP), which then experienced quality control with 2100 Bioanalyzer (Agilent Technologies Santa Clara, US). Afterwards, the total RNA was purified with RNAClean XP Kit (Cat A63987, Beckman Coulter, Inc. Kraemer Boulevard Brea, CA, USA) and RNase-Free DNase Set (Cat #79254, QIAGEN, GmBH, Germany). Library was constructed in steps: rRNA removal, fragmentation, synthesis of 1st and 2nd strand of cDNA, end repairment, poly-A appending at 3'-end, adapter ligation, and enrichment. These steps were completed with VAHTS Total RNA-Seq Library Prep Kit for Illumina (Vazyme), Agencourt AMPure XP Beads (Beckman), SuperScript II Reverse Transcriptase (Invitrogen), Qubit dsDNA HS Array Kit (Invitrogen), and Agilent High Sensitivity DNA Kit (Agilent).
attributes.tag: GEO Accession
attributes.value: GSM3754033