title: GSM2334677: CMK6_Prime_MS091_029; Macaca fascicularis; RNA-Seq
library_construction_protocol: Total RNA was extracted from Cm somatic cells, prime state Cm PSCs, Naive-like state Cm PSCs, and differentiated Cm PSCs by using an RNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. Synthesis and amplification of cDNAs using 1 ng of purified total RNA, and construction of cDNA libraries for RNA sequencing (ABI SOLiD 5500XL system; Life Technologies) were performed as described in (Nakamura et al., 2015). cDNA synthesis and amplification from isolated RNAs/single cells were perfomed essentially as reported in [Kurimoto, K.et.al., Nucleic Acids Research, 34(5), e42.]. Library constructions for Nextseq500 were performed as reported in [Ishikura et al. submitted].
attributes.tag: GEO Accession
attributes.value: GSM2334677