title: GSM2149959: s03_160116_21721_KARPAS299_DMSO_RNA; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the RNeasy mini kit from Qiagen after treatment of the cells with Crizotinib or its vehicle (DMSO). Terminal deoxynucleotidyl transferase was used to add poly(A) tail to the end of the first-strand cDNA which were amplified by 20 cycles of PCR. The libraries were sequenced on Illumina HiSeq 2000 (paired-end, long-read sequencing). Hundred nanograms of cDNA (0.5-3kb) were sheared into 80-130bp fragments. Adaptors were ligated to each end then the fragments were subjected to 10 cycles of PCR amplification. The libraries were sequenced on Illumina HiSeq 2000 (paired-end, long-read sequencing).
attributes.tag: GEO Accession
attributes.value: GSM2149959