title: GSM2756669: ES-Ctli-H3K27ac_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIPSeq,cells were cross-linked in 1% formaldehyde and lysate to extract nuclei. The chromatin was sonicated to 250 bp fragments.  Lysates were cleared from sonicated nuclei and used for immunoprecipitation. For RNASeq, RNA was extracted according to TRIzol protocol (invitrogen). For ChIPSeq,  10ng of immuno-precipitated DNA fragments were used to prepare ChIP-seq libraries with  Ovation SP Ultralow DR Multiplex system (Nugen) following the manufacturer’s protocol. the NEBNext RNA library prep kit (New England BioLabs) and Ovation SP Ultralow DR Multiplex system (Nugen) were used to process RNA-Seq samples following the manufacturer’s protocol.
attributes.tag: GEO Accession
attributes.value: GSM2756669