title: Rota Corvus kubaryi 21-23-1
design: Kapa Library prepared with Nextera-style index sequences. 2 x 150 bp paired-end sequenced. Due to file truncation, 123,211 reads were lost from the ~2.9 million reads in the original untrimmed sequence data of the file 21-23-1_S87_L002_R1_001.fastq.gz. The lost reads were replaced with corresponding trimmed reads (using: Trimmomatic v 0.36 PE ILLUMINACLIP:Nextera-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:20 MINLEN:36). 22,181 reads with trimmed length less than 36 bp were discarded by Trimmomatic. These have been replaced by sequences of 10Ns with quality scores of 0 to maintain sequence pairing integrity