title: AMPLICON
design: DNA extraction from environmental samples using phenol-chloroform  protocol, PCR amplification of 16S rRNA gene variable region (V4  V7) and library construction following Illumina sequencing protocol.       GAGTCACT,GATCCATG,GAGTCAGA,GATCCTAG,GAGTCTCA,GATCCTTC,GAGTCTGT,GATCGAAG,GAGTGACA,GATCGATC,GAGTGAGT,GATCGTAC,GAGTGTCT,GATCGTTG,GATCTCCT,GATCTCGA,GAGAGTGT,GAGTTCAC,GATCTGCA,GAGATCAG,GAGTTCTG,GAGATCTC,GAGTTGAG,GAGATGAC,GAGTTGTC,GAGATGTG,GATCACCA,GAGTACAG,GATCACGT,GAGTACTC,GATCAGCT,GAGTAGAC,GATCAGGA,GAGTAGTG,GATCCAAC,GATCTGGT,GAGTGTGA