title: GSM2415927: ACSS2- Trained Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: The animals were sacrificed 30 minutes following training, and the dorsal hippocampus was dissected on ice. To generate libraries for RNA-seq, polyA+ RNA was extracted using the Dynabeads mRNA Direct kit (Ambion) according to the manufacturer's instructions. RNA-seq libraries for were made using a ScriptSeq v2 RNA-seq Library Preparation Kit from Epicentre (now Illumina). The quantity and quality of the libraries were assessed by BioAnalyzer (Agilent) and qPCR (Kapa Biosystems). The multiplexed libraries were pooled and sequenced on the Illumina NextSeq 500 platform.
attributes.tag: GEO Accession
attributes.value: GSM2415927