title: GSM4678051: ovarian follicular - bad group1; Homo sapiens; miRNA-Seq
library_construction_protocol: Ovarian follicular fluid was collected by transvaginal ultrasound-guided puncture. Immediately after oocytes were retrieved, follicular fluid was collected and processed to remove the blood. Follicular fluid was then centrifuged at 2000g for 10 min to collect the supernatant, and the supernatant was centrifuged twice at 100,000g for 70 min to pellet exosomes. MicroRNA libraries were prepared using the TruSeq Small RNA Sample Prep Kit (Illumina Inc., San Diego, CA, USA) following the manufacturer's instructions. Briefly, small RNAs were ligated with 3' and 5' adapters, followed by reverse transcription to produce single stranded cDNAs. The cDNAs were amplified by PCR in 11 cycles.
attributes.tag: GEO Accession
attributes.value: GSM4678051