title: GSM2677634: HBTEC_NOAA_mRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Trizol reagents (Ambion #15596026) and treated with TURBO DNase (Ambion #AM2239). 1 μg of total RNA was used to prepare sequencing library using the Illumina TruSeq RNA sample preparation kit. A mixture of external RNA spike-in controls (Ambion #4456740) was added to total RNA prior to library preparation. Libraries were sequenced with Illumina Hi-seq platforms to obtain 50 bp-long reads. No spike-in control was added to biological replicate 2, and 100 bp-long reads were obtained from those two libraries.  For chromatin RNA-seq, chromatin subcellular fractionation was isolated. Total RNA-seq libraries were prepared and sequenced with Illumina Hi-seq platforms to obtain 50 bp-long reads.
attributes.tag: GEO Accession
attributes.value: GSM2677634