title: Impact of the chromatin remodeling factor CHD1 on gut microbiome composition of Drosophila melanogaster: Sample_008_Chd1WT/WT
design: DNA was extracted from gut bacteria/microbiomes isolated from Drosophila virgin female Chd1-/- and Chd1WT/WT flies (pool: n=10 per sample). Additionally one “blank” negative control (buffer only) was prepared in parallel. Preparation: 16s rRNA V1+V2 amplicon libraries were prepared with the Ion torrent amplicon fusion primer method (incl barcoding) and sequenced using the Ion Torrent PGM at the Innsbruck Med Uni GenomeSeq core. Barcodes: BC1 (CTAAGGTAAC): Chd1-/- pool1; BC2 (TAAGGAGAAC): Chd1-/- pool2; BC3 (AAGAGGATTC): Chd1-/- pool3; BC7 (TTCGTGATTC): Chd1WT/WT pool1; BC8 (TTCCGATAAC): Chd1WT/WT pool2; BC9 (TGAGCGGAAC): Chd1WT/WT pool3; BC12 (TAGGTGGTTC): no-template-control.