title: GSM2676913: Im-Input; Mus musculus; RIP-Seq
library_construction_protocol: Total RNA of BMDCs was isolated with Trizol regent (Invitrogen) and mRNA was enriched by Dynabeads mRNA Purification Kit (Invitrogen). Removal of ribosomal RNA was confirmed by 2200 Tape Station detection (Agilent). About 8μg mRNA was fragmented to 100-200bp in length and 1/10 volume was separated as input. The remaining fragmented RNA was mixed with dynabeads protein A (Life technology) pre-mixed with anti-m6A antibody overnight. The library preparation was constructed according to TruSeq RNA Sample Preparation Kit v2 (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM2676913