title: WGS of Tenualosa ilisha : Adult fin tissue
design: Sample QC: DNA purity was evaluated by NanoDrop 2000 Spectrophotometer Fluorometer (Life Technologies, Carlsbad, CA, USA) with the ratio of A260/A280 and A260/A230.  DNA quantity was quantified using Qubit 2.0 Fluorometer and Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA). DNA integrity was checked with 0.6% agarose gel with 50 ng sample loaded in each well. PCR-Free DNA Sequencing Library Preparation: For PCR-Free DNA sequencing library preparation, Illumina TruSeq DNA PCR-free Library Preparation Kit was used by following the manufacturer’s recommendations (Illumina, San Diego, CA, USA). Briefly, 1 ug genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument.  The DNA was then end repaired and adenylated, followed by adapters ligated after adenylation of the 3’ends.  Library size was selected following the 350 bp insert size scheme.  DNA libraries was validated using TapeStation (Agilent Technologies, Palo Alto, CA, USA), and was quantified using DNA quantity was quantified using Qubit 2.0 Fluorometer and Qubit dsDNA HS Assay Kit. HiSeq Sequencing: The DNA libraries were quantified by real time PCR (Applied Biosystems, Carlsbad, CA, USA), using KAPA Library Quantification kit.  DNA libraries were pooled and clustered onto 2 lanes of flow cell.  Sequencing was performed on the Illumina HiSeqX instrument according to manufacturer’s instructions.  The samples were sequenced using a 2x 150 paired-end (PE) configuration. Image analysis and base calling were conducted by the HiSeq Control Software (HCS) on the HiSeq.