title: GSM3019620: EMM_nitro_2; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: Total RNA was extracted from 108 cells.  In brief, cells were harvested by 5 minutes of centrifugation at 3000 rpm. Pellets were resuspended in TES (10 mM TrisHCl pH7.5, 10 mM EDTA and 0.5% SDS) and transferred to 65˚C preheated acidic phenol-chloroform (Sigma P-1944). After 1 hour of incubation at 65˚C with mixing every 10 minutes, RNA was extracted with chloroform-isoamyl alcohol (Sigma C-0549), ethanol precipitated and resuspended in water. CAGE libraries were prepared as in Takahashi, H., Lassmann, T., Murata, M. & Carninci, P. 5' end-centered expression profiling using cap-analysis gene expression and next-generation sequencing. Nat Protoc 7, 542–561 (2012).
attributes.tag: GEO Accession
attributes.value: GSM3019620