title: RAD-Seq in O. tshawytscha from Methow River, WA & Warm Springs, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsZnlo11_1888 Warm Springs RL-0088 GTTGGA  OtsZnlo11_2016 Warm Springs RL-0088 TCTCGG  OtsZnlo11_2019 Warm Springs RL-0088 TTTGTC  OtsZnlo11_91 Warm Springs RL-0088 GCTCAA  OtsZnlo12_0146 Warm Springs RL-0088 AATGAG  OtsZnlo12_0172 Warm Springs RL-0088 AGTAGG  OtsZnlo12_0563 Warm Springs RL-0088 CCATTT  OtsZnlo12_0657 Warm Springs RL-0088 CTACAG  OtsZnlo12_0662 Warm Springs RL-0088 GCAGAT  OtsZnlo12_0723 Warm Springs RL-0088 GTAAGT  OtsZnlo12_0937 Warm Springs RL-0088 CTCGCC  OtsZnlo12_1101 Warm Springs RL-0088 TCAAAG  OtsZnlo12_1141 Warm Springs RL-0088 TTATGA  OtsZnlo12_1190 Warm Springs RL-0088 ACAAGA  OtsZnlo12_1283 Warm Springs RL-0088 AGTTAA  OtsZnlo12_2015 Warm Springs RL-0088 GCATGG  OtsZnlo12_2018 Warm Springs RL-0088 GTATCC  OtsZnlo12_2037 Warm Springs RL-0088 TCCTGC  OtsZnlo12_2064 Warm Springs RL-0088 TTCCGT  OtsZnlo12_2122 Warm Springs RL-0088 ACAGCG  OtsZnlo12_2182 Warm Springs RL-0088 ATAGTA  OtsZnlo12_2206 Warm Springs RL-0088 CCCTAA  OtsZnlo12_2240 Warm Springs RL-0088 CTGCGA  OtsZnlo12_2312 Warm Springs RL-0088 GCCGTA  OtsZnlo12_2324 Warm Springs RL-0088 GTCATC  OtsZnlo12_2684 Warm Springs RL-0088 CCCGGT  OtsZnlo12_3175 Warm Springs RL-0088 TCGATT  OtsZnlo12_3183 Warm Springs RL-0088 TTCTAG  OtsZnlo12_3184 Warm Springs RL-0088 ACATAC  OtsZnlo12_3196 Warm Springs RL-0088 ATCAAA  OtsZnlo12_3205 Warm Springs RL-0088 CCGAGG  OtsZnlo12_3215 Warm Springs RL-0088 CTGGTT  OtsZnlo12_3333 Warm Springs RL-0088 GCGACC  OtsZnlo12_3364 Warm Springs RL-0088 GTGCCT  OtsZnlo12_3411 Warm Springs RL-0088 TCGCCA  OtsZnlo12_3424 Warm Springs RL-0088 TTGAGC  OtsZnlo12_3428 Warm Springs RL-0088 ACCATG  OtsZnlo12_3457 Warm Springs RL-0088 ATGCAC  OtsZnlo12_3480 Warm Springs RL-0088 CCGCAT  OtsZnlo12_3496 Warm Springs RL-0088 CTTATG  OtsZnlo12_3526 Warm Springs RL-0088 GCGCTG  OtsZnlo12_3773 Warm Springs RL-0088 GTGTAA  OtsZnlo12_3869 Warm Springs RL-0088 TCGGAC  OtsZnlo12_3878 Warm Springs RL-0088 TTTAAT  OtsZnlo12_3879 Warm Springs RL-0088 ACCCCC  OtsZnlo12_3894 Warm Springs RL-0088 ATGTTG  OtsZnlo12_3962 Warm Springs RL-0088 CCTAAC  OtsZnlo12_4000 Warm Springs RL-0088 CTTTGC  BonnCH041610-1.06 Methow River RL-0088 GGGCGC  BonnCH041610-3.02 Methow River RL-0088 TACACA  BonnCH042110-1.06 Methow River RL-0088 GACGAC  BonnCH042210-2.05 Methow River RL-0088 CACCTC  BonnCH042210-3.05 Methow River RL-0088 AAGACG  BonnCH042310-2.09 Methow River RL-0088 CGGTCC  BonnCH042810-1.03 Methow River RL-0088 TGCCCG  BonnCH043010-1.10 Methow River RL-0088 GGGGCG  BonnCH050211-2.08 Methow River RL-0088 CAATCG  BonnCH050510-4.10 Methow River RL-0088 AGCGCA  BonnCH050511-4.01 Methow River RL-0088 TAAGCT  BonnCH050610-5.02 Methow River RL-0088 TGACAA  BonnCH051010-1.05 Methow River RL-0088 AAGCTA  BonnCH051111-2.05 Methow River RL-0088 GGCAAG  BonnCH051111-4.03 Methow River RL-0088 CAAAAA  BonnCH051210-1.08 Methow River RL-0088 TACGGG  BonnCH051211-1.04 Methow River RL-0088 GAAATG  BonnCH051211-3.07 Methow River RL-0088 ACTGGC  BonnCH051211-5.03 Methow River RL-0088 AACTGA  BonnCH051311-5.08 Methow River RL-0088 AGCCAT  BonnCH051710-1.07 Methow River RL-0088 GACTCT  BonnCH051711-4.04 Methow River RL-0088 GGACTT  BonnCH051811-3.06 Methow River RL-0088 CGAGGC  BonnCH051911-3.03 Methow River RL-0088 GAACCA  BonnCH051911-3.08 Methow River RL-0088 TAATTC  BonnCH051911-4.03 Methow River RL-0088 CGCAGA  BonnCH052311-2.10 Methow River RL-0088 TGAACC  BonnCH052411-1.06 Methow River RL-0088 ATTCCG  BonnCH052411-3.05 Methow River RL-0088 AACGTT  BonnCH052511-5.07 Methow River RL-0088 CGCGTG  BonnCH052711-4.06 Methow River RL-0088 ACTCTT  BonnCH060911-4.03 Methow River RL-0088 TCTTCT  OtsMETH12-008 Methow River RL-0088 AGGGTC  OtsMETH12-009 Methow River RL-0088 CAGGCA  OtsMETH12-012 Methow River RL-0088 CGTCTA  OtsMETH12-014 Methow River RL-0088 GAGAGA  OtsMETH12-017 Methow River RL-0088 GGTACA  OtsMETH12-020 Methow River RL-0088 TAGTAT  OtsMETH12-023 Methow River RL-0088 TGCTTA  OtsMETH12-034 Methow River RL-0088 AATATC  OtsMETH12-201 Methow River RL-0088 AGGTGT  OtsMETH12-202 Methow River RL-0088 CATACT  OtsMETH12-203 Methow River RL-0088 CGTGAT  OtsMETH12-204 Methow River RL-0088 GATCGT  OtsMETH12-205 Methow River RL-0088 GGTTTG  OtsMETH12-206 Methow River RL-0088 TATCAC  OtsMETH12-207 Methow River RL-0088 TGGGGA