title: GSM3169279: shiyan30__L7; Mus musculus; RNA-Seq
library_construction_protocol: 3-month old mouse prostate tissues were got and digested with collagenase type I and 0.25% trypsin/EDTA. Single cell suspension was obtained from passing through a 40µm cell strainer and then FACS sorting for prostate epithelial cells before processing to total RNA extraction, quantification, qualification and finally library preparation. 3ug RNA  were used for the construction of sequencing libraries. Libraries were constructed following the instruction of the Chromium single cell 3' solution (10x Genomics). The libraries were sequenced on Illumina Hiseq X Ten platform. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample.
attributes.tag: GEO Accession
attributes.value: GSM3169279