title: GSM2417740: 2ndOrderSpared_L4_4; Mus musculus; RNA-Seq
library_construction_protocol: Tissue samples originating from the same animal, columns and layer were pooled. Tissue was quickly dissolved in Qiazol (Qiagen #79306) and RNA was isolated using the miRNeasy Mini kit (Qiagen #217004). The extracted RNA was DNase treated (Thermo Scientific, #EN0521) and finally cleaned up using RNeasy MinElute kit (Qiagen #74204). The cDNA library was generated using the non-stranded NEBNext Ultra RNA Library Preparation  Kit for Illumina (New England Biolabs, catalogue #E7530). For the library enrichment, 13-14 PCR cycles were performed
attributes.tag: GEO Accession
attributes.value: GSM2417740