title: GSM3693024: RNA150202CC_6_UD2; Ovis aries; RNA-Seq
library_construction_protocol: Ovine tissue samples for multiomics analysis were obtained immediately after the 2-day experimental protocols. Total RNA was extracted from amnion tissues using an RNeasy Kit (Qiagen, Inc., Valencia, CA). Illumina TruSeq RNA-Seq. Briefly, Poly(A)+ RNA was extracted from total RNA, chemically fragmented and converted to double stranded cDNA by random hexamer priming.  The 3' adenylated fragments were ligated to indexed adaptors.  The libraries were amplified by 10 rounds of polymerase chain reaction (PCR), profiled on the Bioanalyzer and mixed for multiplexing.  Following concentration determination by real time PCR (Kapa Biosystems, Sigma-Aldrich, St. Louis, MO), the mixes were denatured and sequenced on the HiSeq 2000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3693024