title: Treponema validation set A_ 0c_5000copies
design: A mixture of T. pallidum DNA (5,000 16S rDNA copies) and a mock community, which contained no Spirochaetes was not selectively amplified using spirochete specific primers (0c). The V4 region of the 16S rRNA gene was amplified directly for 35c prior to sequencing on the MiSeq Platform.