title: Fine mapping and RNA-Seq unravels candidate genes for a major QTL controlling multiple fiber quality traits at the T1 regionin upland cotton: Yumian1-5DPA
design: In the study, RNA extracted from 0 DPA ovules and 5 DPA fibers was sequenced using an Illumina Hiseq 2500 platform. With the removal of low quality tags, a total of 10 million and 14 million high-quality clean reads were obtained from 0 DPA ovules and 5 DPA fibers mRNA libraries, respectively.Combining the two time-points of cotton fiber development, 457 significantly differentially expressed genes were observed with a consistent high-or-low variation between 0 DPA ovules and 5 DPA fibers. Integration of fine mapping and RNA-Seq is a powerful strategy to uncover candidates for QTL in large genomes.