title: GSM2676348: LIPA+/+ 1-2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA samples were extracted using miRNeasy mini Kit (Qiagen, Valencia, CA). Extracted RNA samples underwent quality control (QC) assessment using the Agilent Bioanalyzer (Agilent, Santa Clara, CA) With a minimum of 100 ng input RNA, we generated first-strand cDNA using random hexamer-primed reverse transcription, followed by second-strand cDNA synthesis using RNase H and DNA polymerase, and ligation of sequencing adapters using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA). Fragments of ~350 bp were selected by gel electrophoresis. To minimize lane and batch effects in RNA-Seq experiments, barcoded libraries were mixed and pooled in equimolar (2 nmol/L) amounts and diluted to 4 pmol/L for cluster formation on a single flow cell lane, followed by 101 bp paired-end sequencing on Illumina’s HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM2676348