title: "neg_control, PCR primers: fwd-ITS1, rev-ITS2": Sample neg_control
design: "Negative control of double-distilled sterile water; PCR targeting ITS region of fungi using MID-tagged primers (fwd: MID-TCCGTAGGTGAACCTGC, rev: GCTGCGTTCTTCATCGATGC); SequalPrep Normalisation; adapter ligation; emPCR"