title: GSM3691992: RR5; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was then isolated using Trizol followed by clean up with RNeasy Minikit (Qiagen) according to manufacturer's protocol and quantified by Bioanalyzer.  All samples had a RNA integrity above 7.0 as determined by Bioanalyzer (Agilent Technologies). Library preparation was performed TruSeq Sample Preparation Kit (Illumina) as per manufacturer's protocols. Final libraries were reassessed for quality (Qubit), multiplexed at six or seven samples per lane (10µM each library), and sequenced on a HiSeq 2500 sequencer (Illumina) at 50bp single-end reads protocol.
attributes.tag: GEO Accession
attributes.value: GSM3691992