title: RAD-Seq in O. tshawytscha from Spring Creek Hatchery, WA
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10ND0002 Spring Creek National Fish Hatchery RL-0060 AATGAG  OtsCORE-10ND0003 Spring Creek National Fish Hatchery RL-0060 AGTAGG  OtsCORE-10ND0004 Spring Creek National Fish Hatchery RL-0060 CCATTT  OtsCORE-10ND0005 Spring Creek National Fish Hatchery RL-0060 CTACAG  OtsCORE-10ND0006 Spring Creek National Fish Hatchery RL-0060 GCAGAT  OtsCORE-10ND0007 Spring Creek National Fish Hatchery RL-0060 GTAAGT  OtsCORE-10ND0008 Spring Creek National Fish Hatchery RL-0060 TCAAAG  OtsCORE-10ND0009 Spring Creek National Fish Hatchery RL-0060 TTATGA  OtsCORE-10ND0010 Spring Creek National Fish Hatchery RL-0060 ACAAGA  OtsCORE-10ND0011 Spring Creek National Fish Hatchery RL-0060 AGTTAA  OtsCORE-10ND0025 Spring Creek National Fish Hatchery RL-0060 CCCGGT  OtsCORE-10ND0026 Spring Creek National Fish Hatchery RL-0060 CTCGCC  OtsCORE-10ND0027 Spring Creek National Fish Hatchery RL-0060 GCATGG  OtsCORE-10ND0028 Spring Creek National Fish Hatchery RL-0060 GTATCC  OtsCORE-10ND0029 Spring Creek National Fish Hatchery RL-0060 TCCTGC  OtsCORE-10ND0030 Spring Creek National Fish Hatchery RL-0060 TTCCGT  OtsCORE-10ND0031 Spring Creek National Fish Hatchery RL-0060 ACAGCG  OtsCORE-10ND0032 Spring Creek National Fish Hatchery RL-0060 ATAGTA  OtsCORE-10ND0033 Spring Creek National Fish Hatchery RL-0060 CCCTAA  OtsCORE-10ND0034 Spring Creek National Fish Hatchery RL-0060 CTGCGA  OtsCORE-10ND0053 Spring Creek National Fish Hatchery RL-0060 GCCGTA  OtsCORE-10ND0054 Spring Creek National Fish Hatchery RL-0060 GTCATC  OtsCORE-10ND0055 Spring Creek National Fish Hatchery RL-0060 TCGATT  OtsCORE-10ND0056 Spring Creek National Fish Hatchery RL-0060 TTCTAG  OtsCORE-10ND0057 Spring Creek National Fish Hatchery RL-0060 ACATAC  OtsCORE-10ND0058 Spring Creek National Fish Hatchery RL-0060 ATCAAA  OtsCORE-10ND0059 Spring Creek National Fish Hatchery RL-0060 CCGAGG  OtsCORE-10ND0060 Spring Creek National Fish Hatchery RL-0060 CTGGTT  OtsCORE-10ND0061 Spring Creek National Fish Hatchery RL-0060 GCGACC  OtsCORE-10ND0062 Spring Creek National Fish Hatchery RL-0060 GTGCCT  OtsCORE-10ND0078 Spring Creek National Fish Hatchery RL-0060 TCGCCA  OtsCORE-10ND0079 Spring Creek National Fish Hatchery RL-0060 TTGAGC  OtsCORE-10ND0080 Spring Creek National Fish Hatchery RL-0060 ACCATG  OtsCORE-10ND0081 Spring Creek National Fish Hatchery RL-0060 ATGCAC  OtsCORE-10ND0082 Spring Creek National Fish Hatchery RL-0060 CCGCAT  OtsCORE-10ND0083 Spring Creek National Fish Hatchery RL-0060 CTTATG  OtsCORE-10ND0084 Spring Creek National Fish Hatchery RL-0060 GCGCTG  OtsCORE-10ND0085 Spring Creek National Fish Hatchery RL-0060 GTGTAA  OtsCORE-10ND0086 Spring Creek National Fish Hatchery RL-0060 TCGGAC  OtsCORE-10ND0087 Spring Creek National Fish Hatchery RL-0060 TTTAAT  OtsCORE-10ND0093 Spring Creek National Fish Hatchery RL-0060 ACCCCC  OtsCORE-10ND0094 Spring Creek National Fish Hatchery RL-0060 ATGTTG  OtsCORE-10ND0095 Spring Creek National Fish Hatchery RL-0060 CCTAAC  OtsCORE-10ND0096 Spring Creek National Fish Hatchery RL-0060 CTTTGC  OtsCORE-10ND0097 Spring Creek National Fish Hatchery RL-0060 GCTCAA  OtsCORE-10ND0098 Spring Creek National Fish Hatchery RL-0060 GTTGGA  OtsCORE-10ND0099 Spring Creek National Fish Hatchery RL-0060 TCTCGG  OtsCORE-10ND0100 Spring Creek National Fish Hatchery RL-0060 TTTGTC