title: RNA-seq  of early instar Drepana arcuata
design: RNA was  extracted from whole bodies of group of early instars (Instars I,II; N=20;  flash frozen using liquid nitrogen) using Norgen Biotek RNA extraction kit  (Norgen Biotek Corp, Thorold, ON, Canada). Larvae from single female were  used in this study, for all the biological replicates. Agilent Bioanalyser  (Santa Clara, CA, United States) was used to assess the quality and quantity  of RNA samples. Library preparation and 150bp paired end sequencing was  carried out using TruSeq RNA v2 Library Prep Kit and Illumina NextSeq500,  respectively at the StemCore sequencing facility (Ottawa Hospital Research  Institute, ON, Canada).