title: Small RNA-Seq of Macaca mulatta: adult male peripheral blood mononuclear cells
design: One microgram of total RNA underwent adapter ligation and primer hybridization before cDNA synthesis (New England Biolabs, Ipswich, MA). cDNA was amplified by PCR and size selected using AMPure XP beads (Beckman Coulter Inc., Brea, CA) to ensure only small RNA molecules are included in the final library. Each library was prepared with unique index primers for multiplexing and subjected to single-end 50 bp sequencing on the HiSeq2500 platform (Illumina, San Diego, CA).