title: GSM3019445: RNAP_76; Escherichia coli; ChIP-Seq
library_construction_protocol: Cell lysates were clarified from sonicated cells and histone-DNA complexes were isolated with an antibody. Purified immunoprecipitated and input DNA was submitted to the University of Wisconsin-Madison Biotechnology Center.  DNA concentration and sizing were verified using the Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA) and Agilent DNA HS chip (Agilent Technologies, Inc., Santa Clara, CA, USA), respectively.  Samples were prepared according the TruSeq® ChIP Sample Preparation kit (Illumina Inc., San Diego, California, USA) with minor modifications. Libraries were size selected for an average insert size of 350 bp using SPRI-based bead selection. Quality and quantity of the finished libraries were assessed using an Agilent DNA1000 chip and Qubit® dsDNA HS Assay Kit, respectively. Libraries were standardized to 2nM.  Cluster generation was performed using standard Cluster Kits (v3) and the Illumina Cluster Station.  Single 100bp sequencing was performed, using standard SBS chemistry (v3) on an Illumina HiSeq2000 sequencer.  Images were analyzed using the standard Illumina Pipeline, version 1.8.2.
attributes.tag: GEO Accession
attributes.value: GSM3019445