title: GSM4565813: ko_rep2; Mus musculus; RNA-Seq
library_construction_protocol: The rostral-most region of the telencephalic primordium was dissected out from DicerF/F and RxCre-DicerF/F E11.5 embryos, and tissue pieces were immediately frozen. Tissue was then dissociated with Papain (Miltenyi Biotech, 130-092-628), RNA extracted using Quick RNA Mini Prep (Zymo Research, R1055) and 1µg of total RNA was used for library preparation and sequencing. Libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit and sequenced on Illumina HISeq 2500 with 100bp paired end read.
attributes.tag: GEO Accession
attributes.value: GSM4565813