title: AMPLICON
design: Total RNA extraction from environmental samples using Trizol RNA extraction protocol, RNA separation, cDNA synthesis from rRNA and PCR amplification of 16S rRNA gene variable region (V4  V7) and library construction following Illumina sequencing protocol.      GATGTCCA, CGAACCTG, GATGTCGT, CGAAGTCA, CCTAGGCA, CGAATGAT, CCTAGGTA, CGACAGCT, CCTATAGC, CGACATAT, CCTCACGT, CGACTGTA, CCTCAGTA, CGAGAATA, CGAGGATG, CGAGTGAC, GATGAGGT, CCTCTCAG, CGATACGT, GATGCAAG, CCTGACAT, GATGCATC, CCTGACGA, GATGCTAC, CCTGAGCA, GATGCTTG, CCTTCGTG, GATGGAAC, CCTTCTGG, GATGGATG, CCTTGATC, GATGGTAG, CCTTGCAT, GATGGTTC, CCTTGGCT, CGATATCT, CCTCGTAC