title: GSM1411215: MCF7 PSF single end ChIP-seq, rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-seq, 10^8 MCF-7 cells (ATCC HTB-22) were crosslinked for 10 minutes with 1% formaldehyde. After nuclei isolation and sonication, sheared chromatin was incubated overnight with protein A beads bound to PSF antisera (clone 39-1; Santa Cruz Biotechnology, sc-101137, Lot # L1012). After incubation, beads were extensively washed and bound DNA was eluted through incubation with proteinase K at 55 degrees C for 1 hour. Eluted DNA was subjected to crosslink reversal overnight at 65 degrees C. DNA was purified using ethanol precipitation and subjected to library construction. Genomic DNA isolated by ChIP enrichment, as well as input DNA, was fragmented using sonication. Sonicated DNA was then subjected to end-repair, A-tailing, ligation to universal adapters, amplification with indexed primers, and size selection using SPRI beads.
attributes.tag: GEO Accession
attributes.value: GSM1411215