title: GSM3754061: S_A_4 [STAT_A4]; Escherichia coli; RNA-Seq
library_construction_protocol: Induced cells were harvested at the precise time point, centrifuge to remove the medium and the pellet snap frozen in liquid nitrogen. Protoplasts were obtained by incubating the pellet in Y1 buffer () for 1 hours at 37C. Total mRNA was extracted using RNeasy Mini Kit. Illumina Ribo-zero rRNA removal reagent was used to deplete the samples of rRNA before library preparation. For the RNA samples paired-end reads were prepared using TruSeq Stranded RNA LT Kit
attributes.tag: GEO Accession
attributes.value: GSM3754061