title: Illumina HiSeq 2500 paired end sequencing; Pericyte induced reprogramming of the bone marrow microenvironment drives stem and cancer cell quiescence
design: Pericyte induced reprogramming of the bone marrow microenvironment drives stem and cancer cell quiescence
library_construction_protocol: This dataset was generated by RNA sequencing of whole tibiae of C57Bl/6 mice. For young mice; tibiae from 6-weeks-old mice were used and for aged mice tibiae from 70-weeks-old mice were used. The control, radiation, and chemotherapy samples were generated using 14-weeks-old mice. For radiation treatment; mice were exposed to sublethal irradiation while for chemotherapy samples mice were treated with carboplatin and tibiae were collected. Adult C57BL/6J wild-type mice were whole-body irradiated with a single dose of 900 rads (Gammacell irradiator) and euthanized 5 days later. For chemotherapy, adult C57BL/6J mice were treated with carboplatin or cisplatin as indicated. Both carboplatin and cisplatin were prepared fresh in saline. Carboplatin was injected in traperitoneally 30 mg/kg/mouse thrice each week for six doses. Qiagen RNeasy Plus Micro Kit Illumina TruSeq RNA Library Preparation Kit v2 with RiboZero
attributes.tag: Experimental Factor: age
attributes.value: 14
attributes.tag: Experimental Factor: compound
attributes.value: carboplatin
attributes.tag: Experimental Factor: dose
attributes.value: 30
attributes.tag: Experimental Factor: irradiate
attributes.value: none