title: GSM3097230: Sample_4h_0nM_pos; Homo sapiens; RNA-Seq
library_construction_protocol: For the preparation of the RNA-Seq libraries for the four cell lines, 10 µg of total RNA was subjected to RiboMinus for the removal of the ribosomal RNA by using the RiboMinus Human/Mouse Transcriptome Isolation Kit (Invitrogen, K155001). After RiboMinus treatment, the samples (~1 µg left) were divided into two equal portions, one treated with RNase R and the other incubated only with the digestion buffer. Samples were cleaned-up by phenol-chloroform before resuspension in a small volume of RNase-free H2O. he library preparation commenced directly with the fragmentation step by mixing the sample and the Fragment, Prime, Finish Mix in the TruSeq Stranded mRNA Library Prep Kit at a 1:1 ratio, followed by the manufacturer's protocol for the remainder of the procedure. RNA-Seq libraries were sequenced as single-end reads in duplicates at ~50 million reads per library using HiSeq 2000 platforms.
attributes.tag: GEO Accession
attributes.value: GSM3097230