title: Treponema validation set C_ 20c_D2
design: 5-fold serial dilution series of T. pallidum ranging from 50,000 to 0.5 copies of 16S rRNA gene was selectively amplified using spirochete specific primers (20c) and subsequently the V4 region of the 16S rRNA gene was amplified for 20c prior to sequencing on the MiSeq Platform.