title: iPSC-RPE RNA: Sample f12d47fe-3a00-4bd7-8ad4-b16c695a9a34
design: Total RNA was isolated using the Quick-RNA Mini Prep Kit (Zymo) from frozen RTL plus pellets, including on-column DNAse treatment step. RNA was eluted in 40 µl RNAse-free water and run on a Bioanalyzer (Agilent) to determine integrity. Concentration was measured by Qubit. Illumina Truseq Stranded mRNA libraries were prepared and sequenced on HiSeq4000, to an average of 40 M 150 bp paired-end reads per sample. 
attributes.tag: alignment_software
attributes.value: STAR