title: Zaire Ebolavirus Strain Mayinga RNA-Seq - 24hpi - Vero Cells
design:  Total RNA was isolated by ising Trizol (Invitrogen) from Vero cells                 (African Green monkey kidney epithelial cells) or differentiated Thp1 cells (human                 monocytic leukemia cells) infected with EBOV Mayinga (808011) or MARV-Ang                 (200501379) at an MOI of 3. Alternatively, total RNA was isolated from tissues of                 MARV-Ang-infected macaques (described below). Poly(A) mRNA was purified from 2 g of                 RNA by using oligo(dT) magnetic beads (Invitrogen), and cDNA libraries were                 established for sequencing on the Illumina HiSeq 2500 platform by using the NEB Next                 mRNA library preparation kit (New England Biolabs). 24 hours post infection Vero                 cells. 