title: RAD-Seq in O. tshawytscha from  Nason Creek, Chiwawa River & White River
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsWENcr09-061009-001 Chiwawa River RL0010 GTTGGA  OtsWENcr09-062309-001 Chiwawa River RL0010 TCTCGG  OtsWENcr09-062509-001 Chiwawa River RL0010 TTTGTC  OtsWENcr09-062709-001 Chiwawa River RL0010 AAACGG  OtsWENcr09-062909-001 Chiwawa River RL0010 ACTCTT  OtsWENcr09-070309-001 Chiwawa River RL0010 ATTCCG  OtsWENcr09-070609-001 Chiwawa River RL0010 CGAGGC  OtsWENcr09-070609-002 Chiwawa River RL0010 TAAGCT  OtsWENcr09-070809-001 Chiwawa River RL0010 GAAATG  OtsWENcr09-071409-001 Chiwawa River RL0010 GGACTT  OtsWENnr08-061508-001 Nason Creek RL0010 ATCAAA  OtsWENnr08-062208-001 Nason Creek RL0010 CCGAGG  OtsWENnr08-062608-001 Nason Creek RL0010 CTGGTT  OtsWENnr08-062808-001 Nason Creek RL0010 GCGACC  OtsWENnr08-070208-001 Nason Creek RL0010 GTGCCT  OtsWENnr08-070808-001 Nason Creek RL0010 TCGCCA  OtsWENnr08-071008-001 Nason Creek RL0010 TTGAGC  OtsWENnr08-071608-001 Nason Creek RL0010 ACCATG  OtsWENnr08-072108-001 Nason Creek RL0010 ATGCAC  OtsWENnr08-072808-001 Nason Creek RL0010 CCGCAT  OtsWENnr09-070909-001 Nason Creek RL0010 TCTTCT  OtsWENnr09-071309-001 Nason Creek RL0010 AACGTT  OtsWENnr09-071809-001 Nason Creek RL0010 ACTGGC  OtsWENwr08-062108-001 White River RL0010 CTTATG  OtsWENwr08-062708-001 White River RL0010 GCGCTG  OtsWENwr08-070108-001 White River RL0010 GTGTAA  OtsWENwr08-070308-001 White River RL0010 TCGGAC  OtsWENwr08-070308-002 White River RL0010 TTTAAT  OtsWENwr08-070408-001 White River RL0010 ACCCCC  OtsWENwr08-070608-001 White River RL0010 ATGTTG  OtsWENwr08-070908-001 White River RL0010 CCTAAC  OtsWENwr08-071308-001 White River RL0010 CTTTGC  OtsWENwr08-071508-001 White River RL0010 GCTCAA  OtsWENwr09-070409-001 White River RL0010 CAAAAA  OtsWENwr09-070809-001 White River RL0010 CGCAGA  OtsWENwr09-071109-001 White River RL0010 GAACCA  OtsWENwr09-071809-001 White River RL0010 GGCAAG