title: WGS of Pseudomonas fluorescens SN15-2
design: First, DNA was quality checked using NanoDrop and Qubit. In the second step, genomic DNA was interrupted to 10 kb with gTube; end-repaired, linker-specific adapters. Attach SMRT dumbbell connector with barcode. Exonuclease digestion was performed to remove fragments not ligated with SMRT dumbbell adapters at both ends; a secondary screening purification was performed using 0.45x of PB magnetic beads to obtain the sequencing library.