title: RAD-Seq in O. tshawytscha from Cape Horn Creek, ID
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  34244-01 Capehorn RL-0091 ACAAGA  34244-02 Capehorn RL-0091 AGTTAA  34244-03 Capehorn RL-0091 CCCGGT  34244-04 Capehorn RL-0091 CTCGCC  34244-06 Capehorn RL-0091 GCATGG  34244-07 Capehorn RL-0091 GTATCC  34244-08 Capehorn RL-0091 TCCTGC  34244-09 Capehorn RL-0091 TTCCGT  34244-10 Capehorn RL-0091 ACAGCG  34244-11 Capehorn RL-0091 ATAGTA  34244-12 Capehorn RL-0091 CCCTAA  34244-13 Capehorn RL-0091 CTGCGA  34244-15 Capehorn RL-0091 GCCGTA  34244-19 Capehorn RL-0091 GTCATC  34244-22 Capehorn RL-0091 TCGATT  34244-26 Capehorn RL-0091 TTCTAG  34244-27 Capehorn RL-0091 ACATAC  34244-28 Capehorn RL-0091 ATCAAA  34244-29 Capehorn RL-0091 CCGAGG  34244-30 Capehorn RL-0091 CTGGTT  34244-31 Capehorn RL-0091 GCGACC  34244-33 Capehorn RL-0091 GTGCCT  34244-35 Capehorn RL-0091 TCGCCA  34244-36 Capehorn RL-0091 TTGAGC  34244-37 Capehorn RL-0091 ACCATG  34244-38 Capehorn RL-0091 ATGCAC  34244-39 Capehorn RL-0091 CCGCAT  34244-40 Capehorn RL-0091 CTTATG  34244-41 Capehorn RL-0091 GCGCTG  34244-42 Capehorn RL-0091 GTGTAA  34244-44 Capehorn RL-0091 TCGGAC  34244-45 Capehorn RL-0091 TTTAAT  34244-46 Capehorn RL-0091 ACCCCC  34244-47 Capehorn RL-0091 ATGTTG  34244-48 Capehorn RL-0091 CCTAAC  34244-49 Capehorn RL-0091 CTTTGC  34244-50 Capehorn RL-0091 GCTCAA  34244-51 Capehorn RL-0091 GTTGGA