title: GSM2333845: Chow 40U, rep1 (DHS-Seq); Mus musculus; DNase-Hypersensitivity
library_construction_protocol: Livers were isolated from mice and nuclei were immediately purified. DNase digestions were performed by adding digestion buffer containing 40U of DNase I (Sigma). DNase I digestion efficiency was evaluated by quantitative PCR53 and samples with optimal digestion efficiency were incubated RNase A (Sigma) before 50–500 bp DNA fragments were purified using ultracentrifugation. DNase-seq libraries were constructed according to manufacturer´s (Illumina) instructions as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279). Purified RNA was subjected to either polydT-enriched cDNA synthesis before sequencing and sequenced on the Illumina platform according to the manufacturer’s instructions. ChIP-seq and libraries of H3K27ac-enriched DNA and DHS-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).
attributes.tag: GEO Accession
attributes.value: GSM2333845