title: LAZ_SEA_Bv6- SEA_0342_20121103_Bv6
design: "PCR amplification of the SSU rRNA gene v6 region using the forward                 primers (967F) CTAACCGANGAACCTYACC, CNACGCGAAGAACCTTANC, ?CAACGCGMARAACCTTACC,                 ATACGCGARGAACCTTACC, and Reverse Primer (1064R)                 ?CGACRRCCATGCANCACCT" 
attributes.tag: nucl_acid_ext
attributes.value: Gentra Puregene Yeast/Bac Kit
attributes.tag: lib_reads_seqd
attributes.value: 1175910
attributes.tag: lib_const_meth
attributes.value: Fusion 2-step amplification
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: v6
attributes.tag: pcr_primers
attributes.value: Forward Primers (967F) CTAACCGANGAACCTYACC CNACGCGAAGAACCTTANC                     ?CAACGCGMARAACCTTACC ATACGCGARGAACCTTACC Reverse Primer (1064R)                     ?CGACRRCCATGCANCACCT
attributes.tag: pcr_cond
attributes.value: 1st step: 95°C for 3 min, 20x(95°C for 30 sec, 60°C for 45 sec and 72°C for                     45 sec), 72°C for 5 min. 2nd step: 95°C for 3 min, 10x(95°C for 30 sec, 60°C for                     45 sec and 72°C for 45 sec) 72°C for 5 min.
attributes.tag: seq_quality_check
attributes.value: PMID: 23799126
attributes.tag: url
attributes.value: http://vamps.mbl.edu/project_pages/ppindex.php?project_code=LAZ_SEA