title: RAD-Seq in O. tshawytscha from Rapid River Hatchery, ID
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  OtsCORE-10NB0001  Rapid River Hatchery RL-0034 AAACGG  OtsCORE-10NB0002  Rapid River Hatchery RL-0034 ACTCTT  OtsCORE-10NB0003  Rapid River Hatchery RL-0034 ATTCCG  OtsCORE-10NB0004  Rapid River Hatchery RL-0034 CGAGGC  OtsCORE-10NB0005  Rapid River Hatchery RL-0034 GAAATG  OtsCORE-10NB0006  Rapid River Hatchery RL-0034 GGACTT  OtsCORE-10NB0007  Rapid River Hatchery RL-0034 TAAGCT  OtsCORE-10NB0008  Rapid River Hatchery RL-0034 TCTTCT  OtsCORE-10NB0009  Rapid River Hatchery RL-0034 AACGTT  OtsCORE-10NB0010  Rapid River Hatchery RL-0034 ACTGGC  OtsCORE-10NB0011  Rapid River Hatchery RL-0034 CAAAAA  OtsCORE-10NB0012  Rapid River Hatchery RL-0034 CGCAGA  OtsCORE-10NB0013  Rapid River Hatchery RL-0034 GAACCA  OtsCORE-10NB0014  Rapid River Hatchery RL-0034 GGCAAG  OtsCORE-10NB0015  Rapid River Hatchery RL-0034 TAATTC  OtsCORE-10NB0016  Rapid River Hatchery RL-0034 TGAACC  OtsCORE-10NB0017  Rapid River Hatchery RL-0034 AACTGA  OtsCORE-10NB0018  Rapid River Hatchery RL-0034 AGCCAT  OtsCORE-10NB0019  Rapid River Hatchery RL-0034 CAATCG  OtsCORE-10NB0020  Rapid River Hatchery RL-0034 CGCGTG  OtsCORE-10NB0021  Rapid River Hatchery RL-0034 GACGAC  OtsCORE-10NB0022  Rapid River Hatchery RL-0034 GGGCGC  OtsCORE-10NB0023  Rapid River Hatchery RL-0034 TACACA  OtsCORE-10NB0024  Rapid River Hatchery RL-0034 TGACAA  OtsCORE-10NB0025  Rapid River Hatchery RL-0034 AAGACG  OtsCORE-10NB0026  Rapid River Hatchery RL-0034 AGCGCA  OtsCORE-10NB0027  Rapid River Hatchery RL-0034 CACCTC  OtsCORE-10NB0028  Rapid River Hatchery RL-0034 CGGTCC  OtsCORE-10NB0029  Rapid River Hatchery RL-0034 GACTCT  OtsCORE-10NB0030  Rapid River Hatchery RL-0034 GGGGCG  OtsCORE-10NB0031  Rapid River Hatchery RL-0034 TACGGG  OtsCORE-10NB0032  Rapid River Hatchery RL-0034 TGCCCG  OtsCORE-10NB0033  Rapid River Hatchery RL-0034 AAGCTA  OtsCORE-10NB0034  Rapid River Hatchery RL-0034 AGGGTC  OtsCORE-10NB0035  Rapid River Hatchery RL-0034 CAGGCA  OtsCORE-10NB0037  Rapid River Hatchery RL-0034 GAGAGA  OtsCORE-10NB0038  Rapid River Hatchery RL-0034 GGTACA  OtsCORE-10NB0039  Rapid River Hatchery RL-0034 TAGTAT  OtsCORE-10NB0040  Rapid River Hatchery RL-0034 TGCTTA  OtsCORE-10NB0041  Rapid River Hatchery RL-0034 AATATC  OtsCORE-10NB0042  Rapid River Hatchery RL-0034 AGGTGT  OtsCORE-10NB0043  Rapid River Hatchery RL-0034 CATACT  OtsCORE-10NB0044  Rapid River Hatchery RL-0034 CGTGAT  OtsCORE-10NB0045  Rapid River Hatchery RL-0034 GATCGT  OtsCORE-10NB0046  Rapid River Hatchery RL-0034 GGTTTG  OtsCORE-10NB0047  Rapid River Hatchery RL-0034 TATCAC  OtsCORE-10NB0048  Rapid River Hatchery RL-0034 TGGGGA