title: RAD-Seq in O. tshawytscha from Catherine Creek, OR
design: Restriction-site associated DNA (RAD) tag sequencing was carried out on genomic DNA samples sheared with the sbfI restriction enzyme.  Samples were sheared in isolation, and unique 6nt barcode sequences were ligated to the sheared sticky ends.  Multiple samples were then pooled into RAD libraries where each sample within a library had a barcode that differed from any other 6nt barcode by at least two bases. Barcoded sequences could then be demultiplexed from raw Illumina sequence data.  Individual name Location RAD_Library Barcode_Sequence  03CHS-CC-01 Catherine Creek RL-0038 AAACGG  03CHS-CC-02 Catherine Creek RL-0038 ACTCTT  03CHS-CC-03 Catherine Creek RL-0038 ATTCCG  03CHS-CC-05 Catherine Creek RL-0038 CGAGGC  03CHS-CC-06 Catherine Creek RL-0038 GAAATG  03CHS-CC-12 Catherine Creek RL-0038 GGACTT  03CHS-CC-13 Catherine Creek RL-0038 TAAGCT  03CHS-CC-18 Catherine Creek RL-0038 TCTTCT  03CHS-CC-20 Catherine Creek RL-0038 AACGTT  03CHS-CC-21 Catherine Creek RL-0038 ACTGGC  03CHS-CC-23 Catherine Creek RL-0038 CAAAAA  03CHS-CC-24 Catherine Creek RL-0038 CGCAGA  03CHS-CC-27 Catherine Creek RL-0038 GAACCA  03CHS-CC-28 Catherine Creek RL-0038 GGCAAG  03CHS-CC-30 Catherine Creek RL-0038 TAATTC  03CHS-CC-31 Catherine Creek RL-0038 TGAACC  03CHS-CC-32 Catherine Creek RL-0038 AACTGA  03CHS-CC-35 Catherine Creek RL-0038 AGCCAT  03CHS-CC-39 Catherine Creek RL-0038 CAATCG  03CHS-CC-43 Catherine Creek RL-0038 CGCGTG  03CHS-CC-46 Catherine Creek RL-0038 GACGAC  03CHS-CC-47 Catherine Creek RL-0038 GGGCGC  03CHS-CC-50 Catherine Creek RL-0038 TACACA  03CHS-CC-52 Catherine Creek RL-0038 TGACAA  03CHS-CC-58 Catherine Creek RL-0038 AAGACG  03CHS-CC-60 Catherine Creek RL-0038 AGCGCA  03CHS-CC-61 Catherine Creek RL-0038 CACCTC  03CHS-CC-62 Catherine Creek RL-0038 CGGTCC  03CHS-CC-63 Catherine Creek RL-0038 GACTCT  03CHS-CC-65 Catherine Creek RL-0038 GGGGCG  03CHS-CC-66 Catherine Creek RL-0038 TACGGG  03CHS-CC-67 Catherine Creek RL-0038 TGCCCG  03CHS-CC-69 Catherine Creek RL-0038 AAGCTA  03CHS-CC-70 Catherine Creek RL-0038 AGGGTC  03CHS-CC-71 Catherine Creek RL-0038 CAGGCA  03CHS-CC-75 Catherine Creek RL-0038 CGTCTA  03CHS-CC-77 Catherine Creek RL-0038 GAGAGA  03CHS-CC-79 Catherine Creek RL-0038 GGTACA  03CHS-CC-81 Catherine Creek RL-0038 TAGTAT  03CHS-CC-82 Catherine Creek RL-0038 TGCTTA  03CHS-CC-83 Catherine Creek RL-0038 AATATC  03CHS-CC-85 Catherine Creek RL-0038 AGGTGT  03CHS-CC-86 Catherine Creek RL-0038 CATACT  03CHS-CC-87 Catherine Creek RL-0038 CGTGAT  03CHS-CC-88 Catherine Creek RL-0038 GATCGT  03CHS-CC-89 Catherine Creek RL-0038 GGTTTG  03CHS-CC-90 Catherine Creek RL-0038 TATCAC  03CHS-CC-96 Catherine Creek RL-0038 TGGGGA