title: ChIP-Seq of estrogen treated liver: 10 weeks old female chicken
design: Briefly, liver tissues were cross-linked with 1% formaldehyde and stopped with 2.0 M glycine. Isolated DNA was fragmented and then incubated with monoclonal specific anti-ER  (0.2 mg/mL, Catalog #: MA5 13065, Invitrogen) or an anti-IgG control (Cell Signaling, Boston, MA, USA). The immunoprecipitated DNA-fragments were quantified by Qubit Fluorometer and used to construct the DNA libraries by using ChIP-Seq Sample Prep Kit (Illumina). The libraries were then sequenced on Illumina HiSeq 2500 using 50bp single end sequencing analysis.