title: RNA-seq of E. coli: _mutS ancestral
design: Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA kit (Illumina, WI). Indexed libraries RNAseq libraries were constructed using strand-specific cDNA synthesis (TruSeq RNA library preparation kit, Illumina), pooled in equimolar concentration and subjected to single-end 50bp Illumina sequencing in an Illumina HiSeq2000 platform using a 2x100 cycles configuration plus 7cycles for index read. All performed in LifeSequencing SL. facilities (Valencia, Spain)