title: GSM3018326: miRNA_scr_direct_1; Mus musculus; miRNA-Seq
library_construction_protocol: PureLink™ miRNA Isolation Kit. For small RNA sequencing, 300ng of small RNA fraction (<200nt) was used as input for TruSeq Small RNA Library Kit (Illumina). Library preparation followed the standard protocol with the exception of skipping the PAGE based size selection before PCR. Instead, complete cDNA pool was amplified and libraries were pooled based on the amount of fragments within the range of 145-160bp that correspond to insert sizes from 15-30 bp. The size selection of this pool was performed with PippinHT machine (Sage Science) on 3% Agarose cassettes with target size set to 154bp.
attributes.tag: GEO Accession
attributes.value: GSM3018326