title: 16S rRNA microbial community analysis: Whole-body Alaskozetes antarcticus males
design: DNA extraction with DNeasy PowerSoil Kit: Sent to Center for Bioinformatics & Functional Genomics at Miami University for 16S rRNA gene sequencing: Concentration determined with a Qubit dsDNA HS Assay kit and a Qubit 3.0 Fluorometer: Earth Microbiome Project primers 515f and 806r to isolate the v4 region of 16S rRNA gene sequences