title: del hns-stpA_POP_DNA-Genomic DNA sequence of the del hns-stpA double deletion mutant generated by lambda red recombinase method
design: We generated the double mutant of hns and stpA  (?hns-stpA) in Escherichia coli  K12-MG1655 using  ?-red recombinase method (Datsenko and Wanner). The double mutant was evolved under laboratory condition at 37°C in LB .  Evolved population collected at  different time points such as ~ 100 hours and ~250 hours were collected and frozen. Genomic DNA was extracted from these evolved  populations using Sigma GenElute™ Bacterial Genomic DNA Kit  (Cat.No. NA2120) using manufacturers instruction. Genomic DNA samples  were prepared for sequencing by using the  Illumina ® TruSeqTM DNA Sample Preparation Kit. Evolved population genomes were sequenced for 50 cycles from one end in Illumina Hiseq1000 platform.