title: Entamoeba histolytica sequencing from HeatShock experiment T2h
design: First passing the cells from 37C to 42C (T=0h) and then  sampling the culture at 2 (T=2h) and 4 (T=4h) hours. An additional 37C  recovering period of 4 h (T=8h) was subsequently sampled.Total RNA was  extracted from approximately 1X106 E. histolytica trophozoites using  Trizol (Invitrogen). The polyA fraction was purified from 10 to 100 g of  total RNA using Dynabeads according to the manufacturers instructions  (Thermofisher ref. 28152103011150). Libraries were constructed using  ScriptSeq mRNA-Seq Library Preparation Kit (Illumina) following  manufacturer's recommendations and were quality controlled using Agilent  Bioanalyzer. Sequencing was performed on a HiSeq 2000 (Illumina) to obtain 58  base single-end reads.