title: Fungal Communities of Ectomycorrhizal Mats from Piloderma and Ramaria Quad 3
design: Individual samples were amplified using the forward primer of 454 Fusion Primer A   8bp Multiplex ID   ITS1F and Reverse primer 454 Fusion Primer B   ITS4.  Each amplification was purified using Qiagen PCR spin columns, quantified with Qubit and pooled in equimolar concentrations.  Sequencing in one direction off Fusion Primer A (ITS1F). Forward Primer: CGTATCGCCTCCCTCGCGCCATCAG MID CTTGGTCATTTAGAGGAAGTAA Reverse Primer: CTATGCGCCTTGCCAGCCCGCTCAGGCTGCGTTCTTCATCGATGC