title: GSM2679764: Pt. 1000 Blood [iRepertoire]; Homo sapiens; OTHER
library_construction_protocol: RNA was purified from CD4+ T cells according to the manufacturer's instructions in the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany). Two rounds of PCR using the iRepertoire human TCR alpha and beta kits (iRepertoire, Huntsville, AL) were performed according to the manufacturer's instructions. Separate reactions were performed for alpha and for beta for each sample. PCR amplicons were generated using primers in the commercial iRepertoire TCR alpha and beta kits, with each patients' alpha and beta TCR products uniquely barcoded after subsequent PCRs.
attributes.tag: GEO Accession
attributes.value: GSM2679764