title: GSM4437263: HEK 293T cells, input (ChIP-seq); Homo sapiens; ChIP-Seq
library_construction_protocol: The chromatin fraction was prepared according to the fanChIP method described in our previous report [Nucleic Acids Research  42:7 4241-4256 (2014)], and DNA/protein complexes were isolated by anti-HA antibody (3F10, Roche). The fanChIP libraries were constructed using a TruSeq ChIP Sample Prep Kit [iIllumina] following the manufacturer's instructions. The quality and quantity of these libraries were checked using Agilent TapeStation D1000 and KAPA Library Quantification Kits [KAPA BioSystems] / Real-time PCR Systems Step One Plus [Applied Biosystems]. The libraries were sequenced on the Illumina HiSeq 2500 System with 50-bp single-end reads according to the manufacturer's protocol at the core facility of Hiroshima University.
attributes.tag: GEO Accession
attributes.value: GSM4437263