title: Illumina NovaSeq 6000 paired end sequencing; The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells
design: The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells
library_construction_protocol: Cells were lysed in Trizol reagent. Cells were cultured in EMEM supplemented with 15% FBS. CD44- and empty-vector-overexpressing IMR90 cells were established by viral transduction and 1 μg/ml puromycin selection. Cells were then incubated for 6 h with PBS or Naked-mole rat skin fibroblasts-derived hyaluronan (cNSF-HA) (20ug/ml). The RNA samples were extracted using Trizol reagent. RNA samples were sent to the Psomagen, Inc. The TruSeq Stranded mRNA  Kit (Illumina) was used for library construction.
attributes.tag: Experimental Factor: genotype
attributes.value: CD44 overexpression
attributes.tag: Experimental Factor: compound
attributes.value: naked mole rat skin fibroblast hyaluronan
attributes.tag: Experimental Factor: dose
attributes.value: 20