title: 16S rRNA amplicon sequencing of human feces from healthy and IBS cohorts for microbial community profiling
design: Genomic DNA was amplified using primers 341F and 805R. Nextera barcodes were attached to forward reads and purity was verified via agarose gel. Products were then purified with a Qiaquick PCR purification kit. Short fragments were removed and the remaining  samples were pooled and then sequenced.