title: DEG analyses of six hulless barley Dulihuang tissues
design: Six different tissues and developmental stages of hulless barley cv. Dulihuang were selected for RNA-seq and digital gene expression (DGE) analyses. The tissues included roots (Ro) and leaves (Le) from seven-day old seedlings, stems from the jointing stage (St), anthers harboring tricellular pollen (An), un-pollinated mature ovaries (Ov), and milky seeds (Ms).  For each tissue sample, total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen).The six RNA samples were sent to BGI (Beijing Genomics Institute, Shenzhen, China) for DEG sequencing analyses (21 bp tags) respectively.