title: mRNA-seq of A. janata midgut_Control_36h
design: Library was prepared using Illumina TruSeq stranded mRNA Library Preparation Kit and as per its described protocol. Briefly, mRNA was enriched from total RNA followed by fragmentation. The fragmented mRNA was converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation and finally ended by limited number of PCR amplification of the adaptor-ligated libraries. Library quantification and qualification was performed using DNA High Sensitivity Assay Kit.