title: GSM3755869: J2; Colossoma macropomum; RNA-Seq
library_construction_protocol: Twenty juveniles were selected based on their sizes (21 to 33 mm standard length), desensitized on ice, sacrificed by medullary section and immersed in RNA later (Qiagen®; Hilden, Germany) immediately after the removal of the head. Total RNA was isolated by the Guanidinium-acid-phenol method (Trizol; Life Technologies®; Carlsbad, USA). Ten best RNA samples were selected based on the quality of the material and the libraries  were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM3755869