title: 16S sequencing of mus musculus gut microbiome: Sample ds02.035
design: "Female C57BL/6J mice received low-dose penicillin (LDP) for 4 weeks (4-LDP, n = 9), 8 weeks (8-LDP, n = 12), or continuous (28 weeks (28-LDP, n = 8).  Controls did not receive LDP (n = 13 ). At 18 weeks of age, after a change in body composition was observed, 3 microbiota donors from each group (control n = 9, LDP n = 8) were selected based on median weight, and cecal contents were transferred to 3-4 week old germ-free Swiss Webster mice Germ-free Swiss Webster mice (control n = 7, LDP n = 8) by oral gavage. After 5 weeks of being conventionalized, cecal contents were recovered from 3 control-microbiota recipients and 3 LDP-microbiota recipients from Trans-LDP1, and transferred to new 3- to 4-week old germ-free Swiss Webster mice (n = 6 each), as described above. Mice were fed HFD, longitudinal fecal pellets were collected, and cecal and ileal samples were collected at sacrifice.  DNA was extracted using the MoBio PowerSoil Kit, and microbial communities were profiled by sequencing the V4 region of the 16S rRNA gene on the MiSeq platorm."