title: GSM1632638: Total_RNAseq_Ezh2_plusTx_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from Ezh2fl/fl cells before and 4 days after treatment with tamoxifen (and Ezh2Δ/Δ cells) with TRizol and DNA removed with DNase-Turbo (Ambion) Ribosomal RNA was depleted from total RNA with Ribo-Zero Gold (EpiCentre). RNA was fragmented using Tris pH 8 (40mM final), magnesium acetate (30mM final),potassium acetate (100mM final), and ends were repaired using Antartic phosphatase and T4 PNK (NEB) according manufacturer instruction.  Libraries were constructed using the Illumina Directional mRNA-Seq Sample Prep and the NEBNext Multiplex Small RNA Library Prep kits.
attributes.tag: GEO Accession
attributes.value: GSM1632638