title: RNA-seq of Rhizopus microsporus infected with btl9-13 mutant Mycetohabitans sp. B13 biorep 2
design: Total RNA isolated using the Qiagen Plant Mini Kit then sent to Novogene for library preparation following their standard construction protocol. mRNA was enriched using oligo(dT) breads and fragmented then reverse transcribed using random hexamer primers. Second strand synthesis is then followed by terminal repair, A ligation, and sequencing adapter ligation to created a cDNA library that is size selected and PCR enriched.