title: Illumina HiSeq 1500 paired end sequencing; Whole Transcriptome sequencing TSCC (tongue squamous cell carcinoma)
design: Whole Transcriptome sequencing TSCC (tongue squamous cell carcinoma)
library_construction_protocol: Stored in RNA later in liqN2 Total RNA was extracted from tongue primary paired normal-tumor samples and cell lines using RNeasy RNA isolation kit (Qiagen) and Trizol reagent (Invitrogen) based methods and later resolved on 1.2% Agarose gel to confirm the RNA integrity. Transcriptome libraries for sequencing were constructed according to the TruSeq RNA library protocol (Illumina). Briefly, mRNA was purified from 4 μg of intact total RNA using oligodT beads and library preparation was done as per manufacturer's instructions (TruSeq RNA Sample Preparation Kit, Illumina).
attributes.tag: Experimental Factor: tongue squamous cell carcinoma
attributes.value: disease
attributes.tag: Experimental Factor: AD0501_23T_tra
attributes.value: individual