title: GSM2679684: Wildtype, high CO2, 0 h before shift to LC H0_3; Cyanidioschyzon merolae strain 10D; RNA-Seq
library_construction_protocol: Cells were harvested and immediately frozen in liquid nitrogen. RNA was isolated ffrom the cells using the EURx GeneMatrix Universal RNA Purification Kit (Roboclon, Berlin, Germany). Residues of DNA were removed with RNase-free DNase I (New England Biolabs). RNA integrity, sequencing library and fragment size were analyzed on a 2100 Bioanalyzer (Agilent). Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina), and quantified with a Qubit 2.0 (Invitrogen). Libraries were prepared from 1 µg RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina). Samples were multiplexed and sequenced in paired-end mode (100 bp read length) on an Illumina HiSeq 2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM2679684