title: NextSeq 500 sequencing; RNA-seq analysis of frs7;frs12;ninja-1 triple loss-of-function seedlings
design: RNA-seq analysis of frs7;frs12;ninja-1 triple loss-of-function seedlings
library_construction_protocol: Whole seedlings were harvested and frozen in liquid nitrogen. Three biological replicates were considered. Wild type Col-0, frs7-1;frs12-1, ninja-1 and frs7-1;frs12-1;ninja-1 lines were sown in MS-agar plates (0.5 MS, no sucrose, 0.8% agar) and stratified for 3 days at 4°C. After stratification, seedlings were grown in SD (8 h light: 16 hours dark) conditions for 10 days. SD (8 h light: 16 hours dark) conditions for 10 days. Seedlings were harvested at ZT8 (2pm) RNA was isolated using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega)  Truseq (Illumina) stranded mRNA library
attributes.tag: Experimental Factor: genotype
attributes.value: frs12-1-frs7-1-frsD