title: DSBCapture of etoposide (0.15uM) induced breaks in GM13069 cells
design: Detection of DNA breaks was performed as previously described (DOI: 10.1038/nmeth.3960), with modification. Briefly,  genomic DNA was carefully purified to avoid/minimize the introduction of DNA breaks in the process. DNA was then subjected to blunting/A-tailing reactions and Illumina adaptor P5 ligation to DNA broken ends. Next, DNA was fragmented by sonication, subsequently ligated to Illumina adaptor P7, and the libraries were amplified by PCR for 15 cycles. Prepared libraries were then subjected to whole-genome 75 bp paired-end sequencing by the Illumina NextSeq 500 platform.