title: Illumina HiSeq 1000 sequencing; Lotus japonicus response to Nod factor: wild type and nfre mutants
design: Lotus japonicus response to Nod factor: wild type and nfre mutants
library_construction_protocol: 3 days seedlings were moved to agar plates supplemented with 1/4 B&D media and susceptible zone of 14 days-old plants was harvested after specific treatment with water(Mock treated) or M.loti Nod factor 10-8M.  The total RNA was isolated from the susceptible zone (15 mm root pieces) using Nucleo spin RNA plant (Macherey-Nagel).  Total RNA (> 0.8 µg) from two biological replicas per sample was used by GATC Biotech (Germany) to prepare random primed cDNA library.
attributes.tag: Experimental Factor: genotype
attributes.value: nfre-1 mutant allele of Nfre
attributes.tag: Experimental Factor: stimulus
attributes.value: water (mock treated)