title: gDNA of N100 - Equus caballus - Sire
design: The shotgun genomic libraries were prepared with the Hyper Library construction kit from Kapa Biosystems. Adaptors have been trimmed from the 3'-end of the reads. The libraries were quantitated by qPCR and sequenced on one lane for 151 cycles from each end of the fragments on a HiSeq 4000 using a HiSeq 4000 sequencing kit Sequence of adaptors used to make the libraries (used portion in bold font for adaptor trimming).   Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina). Adaptor sequence in read1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG (NNNNNN= 6 nt index). Adaptor sequence in read2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT