title: GSM3756732: Hippocampus CA1, Pvalb-cre positive cells, E09 (PatchSeq); Mus musculus; RNA-Seq
library_construction_protocol: After recording from the soma, weak negative pressure was applied in order to aspirate the recorded cell into the glass capillary patch electrode. Using positive pressure the pipette content was quickly ejected into a 1 mL drop of RNase-free lysis buffer (1 mM C1-P1-T31, 10uM dNTP, 10% Triton X, 100mM DTT, 2U/ul Takara Rnase inhibitor) placed on the side of a 0.2 mL RNase free tight-lock tube (TubeOne). The sample was rapidly spun down (5-10 s) and stored at 80 before reverse transcription (RT). Reverse transcription, PCR amplification and sequencing were manually performed in the same way as for Dataset A described in Fuzik et al. Nat Biotechnol. 2016 Feb;34(2):175-183.
attributes.tag: GEO Accession
attributes.value: GSM3756732