title: GSM2420229: Egr1 -/- Rep3; Mus musculus; RNA-Seq
library_construction_protocol: Fresh inguinal subcutaneous fat pads were removed from 2-week-old euthanized Egr1+/+ and Egr1-/- female mice and homogenized using a mechanical disruption device (Lysing Matrix A, Fast Prep MP1, 4 × 30 s, 6 m.s-1). Total RNAs were isolated using the RNeasy mini kit (Qiagen) with 15 min of DNase I (Qiagen) treatment according to the manufacturer’s protocol. Ribosomal RNA depletion was performed with the Ribo-Zero kit (Epicentre), using 500 ng of total RNA. Libraries were prepared using the strand specific RNA-Seq library preparation ScriptSeq V2 kit (Epicentre). 52-78 million of 50-bp strand-specific single-end reads were generated by using a HiSeq 1500 device (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2420229