title: GSM3938437: Homozygous atx1/atx3/atx4 Triple Mutant 2 (TM2) rep7; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: (n=54 per genotype) WT, atx Triple Mutant 1 (TM1), and atx Triple Mutant 2 (TM2) were grown simultaneously. Bolting (1 cm inflorescence) was measured and plants that bolted at the peak of TM bolting (days 24 and 25) were recorded and added to a list. At each time-point, 10 TM1 and 10 TM2 plants were randomly selected from this list and harvested. This strategy was used to ensure that harvested TM plants were developmentally similar. 10 random vegetative WT plants (same age) were selected for harvesting at the each time-point. Harvesting began at the peak of triple mutant bolting and continued three more times in two day increments. Leaf 6 was harvested (N=10 per line, per time point), ground, and homogenized in liquid nitrogen via mortar and pestle, and stored at -80C. The ground powder was divided into three for the production of three RNA-seq libraries (A-C) for each genotype. BrAD-seq, (Townsley et. al.) https://doi.org/10.3389/fpls.2015.00366
attributes.tag: GEO Accession
attributes.value: GSM3938437