title: Illumina MiSeq sequencing; qiita_ptid_2602:10863.348.1.a
design: This project uses the novel WinCF model to test the effect of an oxygen gradient and antibiotic treatment on the structure and function of the cystic fibrosis lung microbiome
library_construction_protocol: Illumina EMP protocol 515fbc, 806r amplification of 16SrRNA V4
attributes.tag: barcode
attributes.value: AGCTATGTATGG
attributes.tag: center_name
attributes.value: UCSDMI
attributes.tag: extraction_robot
attributes.value: HOWE_KF4
attributes.tag: extractionkit_lot
attributes.value: 154050095
attributes.tag: linker
attributes.value: GT
attributes.tag: mastermix_lot
attributes.value: 396036
attributes.tag: orig_name
attributes.value: 348.1
attributes.tag: pcr_primers
attributes.value: FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT
attributes.tag: plating
attributes.value: LDG
attributes.tag: primer
attributes.value: GTGTGYCAGCMGCCGCGGTAA
attributes.tag: primer_date
attributes.value: 1/19/17
attributes.tag: primer_plate
attributes.value: 7
attributes.tag: processing_robot
attributes.value: ROBE
attributes.tag: project_name
attributes.value: CF_Sputum_RobQ
attributes.tag: run_center
attributes.value: UCSDMI
attributes.tag: run_date
attributes.value: 3/13/17
attributes.tag: run_prefix
attributes.value: RQCF_Sputum_S2_L002
attributes.tag: sample_plate
attributes.value: CF_Sputum_RobQ_6
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: tm1000_8_tool
attributes.value: 108379Z
attributes.tag: tm300_8_tool
attributes.value: 208484Z
attributes.tag: tm50_8_tool
attributes.value: 108364Z
attributes.tag: water_lot
attributes.value: RNBF7110
attributes.tag: well_description
attributes.value: CF_Sputum_RobQ_6_348.1_F10
attributes.tag: well_id
attributes.value: F10