title: GSM4506076: shARAF+ARAF_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: After PBS-wash, cells were either detached with EDTA or directly lysed on plate using High Pure RNA Isolation kit (Cat#11828665001, Roche) according to maufacturer´s instructions. Total RNA was quantified by a Qubit 2.0 fluorometer (Invitrogen) and RNA quality and integrity was determined using a RNA6000 Nano chip on Bioanalyzer 2100 (Agilent). Samples with RNA integrity number (RIN) > 8 were further subjected for RNA library preparation. Barcoded cDNA libraries were prepared from 500 ng of total RNA using the NEBnext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). Average size of final cDNA libraries was determined using a HS DNA chip on a Bioanalyzer 2100 and quantity was assesed by Qubit 2.0.
attributes.tag: GEO Accession
attributes.value: GSM4506076