title: H44 ITS2 based analysis of root-asociated fungal communities
design: Metagenomic DNA was extracted from fine roots in temperate forests. Subsequently the ITS2 regions of environmental DNA was amplified using primer pairs of ITS3KYO2 and ITS4. All samples were amplified in triplicate purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq flatform.