title: WT3
design: Fecal pellets were immediately  frozen in liquid nitrogen after collection, and stored at -80oC. Fecal  nucleic acid was extracted from fecal pellets using Fecal genomic extraction  kit (DP-328, Tiangen technology corporation). Barcoded PCR primers (341F and  805R) directed at the V3/V4 region of bacterial 16S rRNA genes were used to  generate libraries and libraries were validated by the Agilent 2100  Bioanalyzer. Sequencing were performed with 250 bp paired-end reads on  Illumina Hiseq2500 platform in Annoroad Gene Technology (Beijng).