title: Seawater Metagenomes: Dry Tortugas National Park
design: The source of this metagenome is a sample of seawater collected in June 2017 from a site in the Dry Tortugas National Park immediately adjacent to a coral colony.  Five liters of water were collected in duplicate by a peristaltic pump.  The action of the pump pushed the water through two inline filters: a 0.8 um prefilter and a 0.22 um Sterivex filter.  After the water was filtered, the Sterivex filters were capped, sealed in plastic bags, and stored in liquid nitrogen until further processing (within 14 days).  Subsequent processing occurred at the Environmental Microbiology Laboratory in St. Petersburg, FL.  Deoxyribonucleic acid (DNA) was extracted from the Sterivex filters using the MO BIO PowerWater Sterivex DNA Isolation Kit following the manufacturer's instructions except for Steps 8 and 9, which were omitted.  Steps 8 and 9 call for the heating and subsequent cooling of the filter.  Extracted DNA from the replicate filters was pooled in order to obtain one extraction for the sample.  The shotgun metagenome (untargeted sequencing of all genomes present in a sample) dataset was produced by construction and sequencing of a Nextera library.  Nextera library construction kits use transposase enzymes to fragment and tag DNA in order to prepare samples for sequencing.  The samples were sequenced on an Illumina HiSeq 4000 sequencing system by Texas A&M AgriLife Genomics and Bioinformatics Service.