title: Illumina HiSeq 3000 paired end sequencing; Cattle transcriptome profiling in cd4 cells by the FAANG pilot project FR-AgENCODE
design: Paired-end RNA-Seq of polyA+ transcripts from cd4 cells on an Illumina HiSeq 3000
library_construction_protocol: TruSeq Stranded mRNA Sample Preparation kit V2 Guide (with a reduction of the fragmentation time from 8 to 2 minutes)
attributes.tag: assay type
attributes.value: transcription profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation, other
attributes.tag: sampling to preparation interval
attributes.value: 40
attributes.tag: experimental protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
attributes.tag: extraction protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_RNA_extraction_20160504.pdf
attributes.tag: library preparation location
attributes.value: Plateforme Genomique de Toulouse, INRA Castanet-Tolosan, France
attributes.tag: library preparation location longitude
attributes.value: 1.499654
attributes.tag: library preparation location latitude
attributes.value: 43.527571
attributes.tag: library preparation date
attributes.value: 2016-08-12T00:00:00
attributes.tag: sequencing location
attributes.value: Plateforme Genomique de Toulouse, INRA Castanet-Tolosan, France
attributes.tag: sequencing location longitude
attributes.value: 1.499654
attributes.tag: sequencing location latitude
attributes.value: 43.527571
attributes.tag: sequencing date
attributes.value: 2016-08-12T00:00:00
attributes.tag: experiment target
attributes.value: polyA RNA
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
attributes.tag: library generation protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
attributes.tag: read strand
attributes.value: mate 2 sense
attributes.tag: rna integrity number
attributes.value: 9.1