title: Illumina HiSeq 3000 sequencing; RNA-seq comparison of CD81 positive and negative cells in subcutaneous and visceral white fat and brown fat tissues in mouse
design: RNA-seq comparison of CD81 positive and negative cells in subcutaneous and visceral white fat and brown fat tissues in mouse
library_construction_protocol: Lin- Sca1+ CD81+ and Lin- Sca1+ and CD81- cells were sorted from scWAT, vWAT and BAT of wildtype C57BL6 mice using FACS. Total RNA was isolated from sorted CD81+ and CD81- cells from wild type C57BL/6 mice at 10-weeks-old using RNeasy Micro Kit (QIAGEN). cDNA synthesis and amplification was performed from 50 ng of total RNA using SMARTer® Universal Low Input RNA Kit (Clontech) according to manufacturer's instruction.
attributes.tag: Experimental Factor: phenotype
attributes.value: CD81 negative
attributes.tag: Experimental Factor: organism part
attributes.value: brown adipose tissue