title: Shotgun metagenomic sequencing of human gut microbiota
design: A frozen aliquot (200 mg) of fecal sample was processed, and bacterial DNA was extracted with the QIAamp PowerFecal DNA Kit (Qiagen). Paired-end metagenomic sequencing was performed on the Illumina NovaSeq 6000 platform with a pair-read length of 150 bp (insert size 350 bp).