title: 16S rRNA of deadwood, sample N7b__2014
design: Amplicons were generated using the primers for 16S hypervariable regions V1-V3 to characterize bacteria (5-GTAGAGTTTGATCCTGGCTCAG-3, 5-CCATTACCGCGGCTGCTGG-3).  Q5 High-Fidelity DNA Polymerase and buffer (New England Biolabs; M0491), hereafter Q5, were used on samples collected in 2014.