title: ITS seq of horse sinus wash
design: barcoded and PCR primers  ITS1F-Bt1 (CTTGGTCATTTAGAGGAAGTAA) and ITS2R (GCTGCGTTCTTCATCGATGC) were used  in a 28 cycle PCR using the HotStarTaq Plus Master Mix Kit and sequenced via  Illumina MiSeq