title: GSM2340748: E14.5, biological replicate 1, single cells, transcriptome 44; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were captured on a Fluidigm C1 RNA-Seq IFC (10-17 μm), and all capture sites were visually inspected under microscope to select sites where only one cell is captured. SMARTer Ultra Low RNA kit for Illumina (Clontech Laboratories) were used for on chip cell lysis, reverse transcription and cDNA amplification. ERCC Spike-in Mix (Ambion) were used as technical control. Sequencing libraries were prepared using Nextera XT Kit and Index Kit (illumina) per the vendor’s protocol.
attributes.tag: GEO Accession
attributes.value: GSM2340748