title: RNA-Seq of IDO3
design: First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase . Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H.  Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. Then the library fragments were purified with AMPure XP system.Then PCR was performed and products were purifiedand library quality was assessed.