title: GSM1412671: P60_wt cones [miRNA-seq]; Mus musculus; miRNA-Seq
library_construction_protocol: Cones were isolated by FACS from D4-cre/Ai9 tdTomato (wild type) mice at P60. RNA extraction was performed with Trizol according to the manufacturer's instructions. Quantitative and qualitative analysis was performed using 2100 Bioanalyzer. Small RNA (15-30 nt in length) libraries were prepared from 1 µg of total RNA using TruSeq Small RNA Sample Prep Kit according to the manufacturer's protocol. Samples were multiplexed and 13 pM of the multiplexed libraries were sequenced on one lane of the HiSeq 2000 instrument using RTA 1.13.48. Each sample was prepared from FACS-sorted cones from 20 retinas.
attributes.tag: GEO Accession
attributes.value: GSM1412671