title: GSM2577223: 18h_Tet-_GTCCGC_L006; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from 10e6 cells using RNeasy mini kit (Qiagen) with a separate step for DNase I digestion (Qiagen). ERCC Spike-in control mix-1 (Life technologies) was added to each sample prior to processing (1 μl of a 1:10 dilution of mix-1). RNA from each sample was processed using the RiboGold kit (Epicentre) to remove rRNA. rRNA-depleted RNA was used to prepare libraries with the Ultra directional RNA library prep kit for Illumina (New England Bioscience) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2577223