title: RNA-Seq of Homo sapiens: chorionic villus sample: Sample RNA-058
design: Total RNA was extracted from chorionic villus samples using TRIzol (Life Technologies) followed by RNeasy Mini cleanup (Qiagen);  mRNA was isolated via poly-A selection and libraries were constructed using the TruSeq Stranded mRNA kit for paired-end sequencing (Illumina).  Multiplexed libraries were sequenced on the Illumina Illumina HiSeq 2000 and/or 2500; libraries were re-sequenced to obtain more reads if necessary.
attributes.tag: alignment_software
attributes.value: GSNAP (version 2013-02-05)