title: GSM2584761: PMN-MDSC from Adult mice, replicate 32; Mus musculus; RNA-Seq
library_construction_protocol: PMN-MDSC and M-MDSC from spleens of neonates (7 day old) and adult mice (6-8 weeks old) were enriched by CD11b-beads and then sorted on FACSAria cell sorter (BD Bioscience) using  CD11b+Ly6CintLy6G+ phenotype or PMN-MDSCs and CD11b+Ly6ChighLy6G- for M-MDSCs VAHTS Total RNA-Seq Library Preparation Kit (Vazyme Biotech Co., Ltd, China) was used for library preparation. Samples were a subject to a 50 bp single-end sequencing run on Illumina HiSeq 2500 following the manufacturer’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2584761