title: GSM1632848: iNKT WT BR1 RNA-Seq thymic iNKT; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from iNKT cells  and CD8 SP T cells was isolated with E.Z.N.A HP Total RNA. The integrity of the isolated RNA was evaluated in the Bioanalyzer (Agilent Technologies Inc). Only samples with a minimum RIN value of 9 were used for library preparation. Libraries were prepared using the Truseq RNA stranded kit (Illumina). Briefly, polyA+ RNAs were selected with magnetic beads, the RNA was fragmented and cDNA was synthesized. After A-tailing and adaptor ligation, libraries were generated by amplifying the cDNA for 10-12 cycles. The quality and quantity of the amplified libraries was evaluated using Bioanalyzer (Agilent Technologies Inc) and HS Qubit (Life Technologies). The libraries were pooled in a concentration of 10 nM and sequenced using an Illumina Hiseq 2500 instrument.
attributes.tag: GEO Accession
attributes.value: GSM1632848