title: Deep-sequence of zebrafish chd gene off target1: non-injection replicate2
design: Target region-containing fragment was first PCR-amplified using 1st primer pairs from the extracted genomic DNA. The second nested PCR was performed to obtain adapter added amplicon by using the first PCR products as template and 2nd primer pairs containing adapter sequences. Then, the amplicon was labeled using 3rd primer pairs containing index sequences.