title: GSM3323546: Nestin-cKO_NS_replica1; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq: Total RNA from cortical cultures was extracted with TRI reagent (Sigma-Aldrich) and digested with DNase I (QIAGEN®). RNA samples were extracted and re-precipitated using acid phenol-chloroform and ethanol.   . ChIP-seq: Primary hippocampal neurons were obtained from E17 embryos. Cultured neurons were infected with syn-VP16-SRF/Syn-GFP viral vector at DIV3. After 7 days post-infection neurons were collected and genomic DNA from 5 × 10^6 hippocampal neurons was fragmented by sonication and immunoprecipitated with anti-VP16 antibody (Santa Cruz, sc-7545) as previously described (Scandaglia et al., 2015). Immunoprecipitated DNA was resuspended in 40 μL of water Single-End stranded Chip and RNA libraries were prepared for sequencing using standard Illumina protocols (HiSeq Sequencing v4 Chemistry), with read length 50 and application Truseq Stranded mRNA.  Service provided by CRG.
attributes.tag: GEO Accession
attributes.value: GSM3323546