title: iso-1 in stress conditions for RNA-seq replicate 2
design: Total RNA from 30 female midguts was isolated using GenElute Mammalian genomic mammalian total RNA miniprep kit from SIGMA following manufacturers instructions. 1,5ug of total RNA from each sample was used for library preparation and sequencing. Library preparation was performed using the Truseq Stranded mRNA Sample Prep kit from Illumina following manufacturers instructions. Sequencing was done in paired-end and 125nts long obtaining a minimum of 25 million pair reads per sample.