title: HS250G1_POP_DNA-Genomic DNA sequence of the population of del hns-stpA evolved for 250 hour under laboratory condition
design: We generated the double mutant of hns and stpA (?hns-stpA) in                 Escherichia coli K12-MG1655 using ?-red recombinase method (Datsenko and Wanner).                 The double mutant was evolved under laboratory condition at 37°C in LB . Evolved                 population collected at different time points such as ~ 100 hours and ~250 hours                 were collected and frozen. Genomic DNA was extracted from these evolved populations                 using Sigma GenElute™ Bacterial Genomic DNA Kit (Cat.No. NA2120) using manufacturers                 instruction. Genomic DNA samples were prepared for sequencing by using the Illumina                 ® TruSeqTM DNA Sample Preparation Kit. Evolved population genomes were sequenced for                 50 cycles from one end in Illumina Hiseq1000 platform.