title: PstI-2 GBS of three pines: megagametophyte_10samples_PAGE: Sample PtFS01_04B
design: The PstI-based genotyping-by-sequencing approach (GBS) was employed to optimize the GBS approach in conifer. For the PstI-2 (P2) library, PAGE gel electrophoresis was use for the size fractionation of restriction fragments. 10 samples were mixed in the library.