title: Illumina HiSeq 2000 paired end sequencing; Analysis of abomasal lymph node transcriptome for the identification of genes and lncRNAs involved in nematode resistance gastrointestinal tract in adult sheep.
design: RNA-Seq transcriptomic paired end experiment using Illumina HiSeq 2000 sequencer
library_construction_protocol: KAPA Stranded mRNA-Seq Illumina(r) Platforms Kit
attributes.tag: assay type
attributes.value: RNA-seq of total RNA
attributes.tag: sample storage
attributes.value: RNAlater, frozen
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 12
attributes.tag: experimental protocol
attributes.value: https://doi.org/10.1186/s13567-018-0534-x
attributes.tag: extraction protocol
attributes.value: https://doi.org/10.1186/s13567-018-0534-x
attributes.tag: library preparation location
attributes.value: Barcelona, Spain
attributes.tag: library preparation location longitude
attributes.value: 41.390205
attributes.tag: library preparation location latitude
attributes.value: 2.154007
attributes.tag: library preparation date
attributes.value: 2017-01-16
attributes.tag: sequencing location
attributes.value: Spain
attributes.tag: sequencing location longitude
attributes.value: 41.390205
attributes.tag: sequencing location latitude
attributes.value: 2.154007
attributes.tag: sequencing date
attributes.value: 2017-02-10
attributes.tag: experiment target
attributes.value: total RNA
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: library generation protocol
attributes.value: http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
attributes.tag: read strand
attributes.value: mate 2 sense
attributes.tag: rna purity - 260:280 ratio
attributes.value: 1.955
attributes.tag: rna purity - 260:230 ratio
attributes.value: 1.93
attributes.tag: rna integrity number
attributes.value: 7.3