title: Evolutionary engineering of a wine yeast strain in low-temperature fermentation
design: Illumina sequencing libraries were constructed from YMD1803:P5, YMD1805:P5-  EM. Genomic DNA was extracted with the Hoffman-Winston DNA prep. Bar-coded   DNA fragment libraries were prepared by a Nextera DNA sample preparation kit   (Epicentre Biotechnologies, Madison, WI) following standard procedures and published   recommendations (Adey et al., 2010).   Briefly, 50 ng of yeast genomic DNA from each strain were tagmented (tagged and   fragmented) by the Nextera transposome. The tagmented DNA was purified following   the AMPure (Agencourt) purification protocol. Purified tagmented DNA libraries   were PCR-amplified with the Nextera PCR Master Mix. PCR-amplified libraries were   cleaned following the AMPure (Agencourt) purification procedures, and submitted for   sequencing.