title: RNA-Seq of RCC: ALI and tissue
design: ALI patient-derived organoids (ALI PDO) were kept in culture until organoid formation. ALI PDOs were treated with collagenase IV and total RNA from ALI PDO and their matched fresh tumor tissue was extracted using the Roboklon Universal RNA Kit (E3598-02). The library was prepared using Lexogen's QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina. 3'mRNA sequencing was performed on Illumina's HiSeq 2500 system.