title: GSM2584478: R540Q PFV vector in HT1080 cells; Homo sapiens; OTHER
library_construction_protocol: Cellular genomic DNA isolated 5 days post-infection was digested with BfaI/BanII/CviQI and ligated to a linker made by annealing oligonucleotides 5´- TAGTAGTCACGAGCGTC (with 3´ end blocked by an amino group modifier) and 5´-CAGTTAGACTACACGTTAGACGGACGCTCGTGACTAC. PFV integration sites were amplified using primers 5´-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCAGTTAGACTACACGTTAGACG and 5´-GTGTGAACTACACTTATCTTAAATGATG followed by a nested PCR with primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCAGTTAGACTACACGTTAGACG and 5´- AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXCTTAAATGATGTAACTCCTTAGGATAATCAATATAC (the string of underlined nucleotides marked with “X” denotes the position of a 5-base index, which was unique for each integration site library analyzed).
attributes.tag: GEO Accession
attributes.value: GSM2584478