title: GSM2759910: dBigH1_ChIPseq_embryo; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Embryos were collected in peach juice plates at 0-2 hours after fertilization (for details see Materials and Methods section in Pérez-Montero et al., 2013). ChIP-seq, library construction, cluster generation and Sequencing analysis were performed using the Illumina HiSeq2000, sequencing type SR50, following manufacture's protocols (www.illumina.com). In brief, libraries were prepared using Illumina's ChIP-Seq Sample Prep Kit from 3.5 ng of ChIP/Input DNA. Samples were size selected to ̴300-350 bp using DNA magnetic beads. Adaptor-modified DNA fragments were subjected to limited PCR amplification (18 cycles). Using Illumina's Cluster Generation Kit, libraries were subject to cluster generation for subsequent sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2759910