title: GSM4193227: Metformin; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cultured H1299 cells using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA). DNA contamination was removed using DNase, and the remaining RNA was broken into short fragments. The RNA fragments were reverse transcribed into cDNA, ligated with adaptors, and PCR amplified. The amplified cDNAs were then selected by a range of sizes and sequenced using a next-generation sequencing device (NovaSeq 6000 System). The sequence library was mapped to the reference genome UCSC hg19 using the Bowtie2 program.
attributes.tag: GEO Accession
attributes.value: GSM4193227