title: GSM4506043: ATAC_IL23; Mus musculus; ATAC-seq
library_construction_protocol: Skin ILCs were isolated from ears of WT naïve mice (day 0) and rIL-23 treated mice (day 4) with n=10 mice per group. Total skin ILCs were sorted (approximately 60,000 cells per group) as CD45.2, CD90.2 positive, lineage, TCRgamma and CD3epsilon negative. After sorting, cells were immediately washed and resuspended in 1ml of CryoStore CS10 freezing media (StemCell Technologies) followed by cryopreservation at -80°C with cooling rate of 1°C/min using Mr. Frosty (Nalgene). For scATAC-seq cells were thawed, washed and lysed for 3 min on ice according to the low input protocol recommendations by 10x Genomics (CG000169-Rev C). For single cell library preparation, the 10x Genomics Chromium Controller and the 10x Genomics Chromium Single Cell ATAC Library & Gel Bead Kit (#1000111) were used, according to the manufacturer's instructions (CG000168-Rev B). 10x ATACseq v1.0
attributes.tag: GEO Accession
attributes.value: GSM4506043