title: Shotgun metagenome of child human gut: fecal sample
design: DNA was isolated in two stages; first lysing the samples in Lysis Matrix E tubes on a Precellys Tissue Homogenizer, and second using the MagAttract PowerMicrobiome DNA/RNA EP Kit (Qiagen) in an Eppendorf epMotion 5073 automated liquid handling system.  Metagenomic libraries were prepared using 30l DNA in a 96-well plate with the Nextera DNA Flex Library Prep Kit (Illumina).  The library preparation was initiated with tagmentation and post-tagmentation cleanup followed by amplification of tagmented DNA and cleanup using Sample Purification Beads for both the cleanup steps. Library quality control included quantification of the final library using the Qubit dsDNA High Sensitivity assay and library quality assessment using the Agilent High Sensitivity HS D5000 ScreenTape assay on the Agilent 4200 Tapestation.  The individual libraries were diluted and pooled at a concentration of 7nM, to make the final library pool. This pool was normalized to 1.8pM, spiked-in with 1% PhiX sequencing control and sequenced on an Illumina NextSeq 500 instrument.