title: GSM2584570: nlg-1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: L4  worms were harvested from NGM plates,  frozen with TRIReagent and total RNA purified  and treated with DNase I (Thermo Fisher Scientific) . RNA quality and integrity was  evaluated with the Experion Bioanalyzer (Bio - Rad). Polyadenylated - RNA was purified from total RNA using Dynabeads mRNA DIRECT kit ( Thermo Fisher Scientific ). Purified mRNA was used for preparing cDNA (strand - specific) libraries using the Ion Total RNA - Seq  Kit v2 kit ( Thermo Fisher Scientific). These libraries were run through the Ion Personal Genome Machine® (PGM™) System using the Ion 318™ Chip Kit v2 (Thermo Fisher Scientific ).
attributes.tag: GEO Accession
attributes.value: GSM2584570