title: Illumina HiSeq 2500 paired end sequencing; Isoform-specific depletion of U2AF1
design: Isoform-specific depletion of U2AF1
library_construction_protocol: Cell lines were grown under standard conditions in DMEM supplemented with 10% (v/v) bovine calf serum (Gibco BRL) Transfections of small interfering RNAs (siRNAs) were carried out in 6- or 12- well plates using jetPRIME (Polyplus) according to manufacturer's recommendations. The cells received treatment with siRNA twice - 24h post seeding, and 48h post seeding when splitting into a new plate. A human/mouse/rat Ribo-Zero rRNA Removal Kit (Cambio/Epicentre) was used according to the manufacturer's specifications.
attributes.tag: Experimental Factor: library selection
attributes.value: Ribosomal Depletion
attributes.tag: Experimental Factor: transfection
attributes.value: U2AF1 a siRNA