title: GSM4002963: H3K27ac_HiChIP_NIH3T3_1; Mus musculus; OTHER
library_construction_protocol: H3K27ac HiChIP was performed according to (Mumbach et al., 2017) with only minor modifications. Approximately 15 million NIH3T3 cells were used as input, and MboI digestion was carried out for 2 hours. We used anti-H3K27ac (Abcam, ab4729) . Cells were sonicated for 10 cycles (30 seconds on, and 30 seconds off) using a Bioruptor Pico instrument (Diagenode). For biotin pull down 150ng of chromatin was used as input, and tagementation was performed using 4 uL of Tn5 transposase. For post-PCR size selection we performed a double-sided size selection with Ampure XP beads (Beckmann-Coulter). All libraries were sequenced on a NextSeq 500 platform.