title: GSM4505018: PANC-1-low-Lovastatin-3; Homo sapiens; RNA-Seq
library_construction_protocol: ~4×10^6 cells were havested and total RNA were extracted with RNeasy Mini Kit (Qiagen, Valencia, CA). The mRNA was purified and fragmented from total RNA (2 μg) using poly-T oligo-attached magnetic beads with two rounds of purification. Cleaved RNA fragments primed with random hexamers were reverse transcribed into first-strand cDNA using reverse transcriptase and random primers. The RNA template was removed and synthesized a replacement strand to generate double-strand cDNA. End repair, A-tailing, adaptor ligation, cDNA template purification and enrichment of the purified cDNA templates using PCR were then performed. Constructed libraries were 150 bp paired-end sequenced by an Illumina X10 sequencer, following manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4505018