title: GSM3392374: Sample 342_HE10W_TL_sc3; Homo sapiens; RNA-Seq
library_construction_protocol: The dissected tissue sections were digested in 2mg/ml collagenase IV (Gibco, 17104-019), 1 mg/ml papain (Sigma, P4762) and 10 U/μl DNase I (NEB, M0303L) with rough pipetting and then vortex on thermocycler at 37°C for 15 min to get single cells. We did barcoded single cell transcritome library preparation the same as previous studies. The 3' end of the mRNAs were captured in the libraries. Specifically, 4 cycles of pre-amplification with 98°C 20s, 65°C 30s and 72°C 5min was carried out before 14 cycles of cDNA amplification with  98°C 20s, 67°C 15s and 72°C 5min in this study. barcoded-single cell RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM3392374