title: Soil microbiome (16S):  Traditional Agroecosystem: Bulk soil control: sample 6
design: DNA extraction: 0.25 g  with PowerSoil (R); DNA  adjusted to 20 ng/uL; PCR: 1uL as template, 16S primers F515 and R806 (V4 region) + adaper, primer pad, linker and barcode; PCR products pooled and gel-purified, mixed with 1:1 PhiX; Sequencing: 500 cycle, Illumina MiSeq kit V2; Demultiplexing: QIIME 2.