title: Illumina HiSeq 2500 sequencing; RNA-seq of wild-type and Tbx20 homozygous knockout E11.5 mouse cardiomyocytes to study Tbx20's roles in embryonic and adult mouse heart function
design: RNA-seq of wild-type and Tbx20 homozygous knockout E11.5 mouse cardiomyocytes to study Tbx20's roles in embryonic and adult mouse heart function
library_construction_protocol: The mice in this experiment were E11.5 control (Tnnt2-rtTA;TetO-Cre;Tbx20wt/loxP) and homozygous mutants (Tnnt2-rtTA;TetO-Cre;Tbx20loxp/loxP  after induction with Doxycycline at E8.5.  Hearts were harvested and dissociated using enzymatic digestion by collagenase (10mg/ml; Worthington) and dispase (10mg/mL, Invitrogen). Live Tnnt2-rtTA;TetO-Cre;R26-tdTom lineage traced cardiomyocyte cells were sorted on Influx Cell sorter (BD Biosciences) and collected in TRIZol reagent (Ambion) for RNA extraction. Cardiomyocyte specific Tbx20 conditional mutants were generated by breeding Tnnt2-rtTA;TetO-Cre;Tbx20+/loxp males with Tbx20loxP/loxP;R26tdTom/tdTom females. Cre expression  was induced by providing Doxycycline (MP Biomedicals, cat# 198955; 1mg/mL) in drinking water to pregnant females from E8.5 onwards. Illumina library protocol
attributes.tag: Experimental Factor: wild type genotype
attributes.value: genotype