title: RRBS Gasterosteus aculeatus: muscle tissue, adult, warm acclimated
design: genomic DNA isolated from threespine stickleback muscle tissue was digested with the methylation sensitive restriction enzyme MspI. MspI digested gDNA served as the input material for the Bio-O NEXTflex Bisulfite-Seq Kit, with the following steps: End Repair, Adenylation, Adapter Ligation (NEXTflex Bisulfite-Seq Barcodes), Gel-Free Size Selection (Agencourt AMPure XP Beads). The purified adapter ligated DNA was then subjected to Bisulfite Conversion (Zymo Research EZ DNA Methylation Gold Kit)