title: GSM4005179: mESC_DMSO_H3K27me3_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: formaldehyde fixed chromatin was preprocessed following RELACS high-thoughput ChIP-seq protocol (Arrigoni et al., 2019) using Covaris sonicator. For end repair, a-tailing, barcoding, and adaptor ligation we use reagents from the NEBNext Ultra II kit. RNA was extracted using RNeasy Mini Kit and processed using TrueSeq Stranded mRNA kit.  ATAC-seq was generated following the protocol of Buerostro et al. 2015. Libraries were prepared according to Illumina's instructions using NebNext Ultra II kit. After illumina adapter ligation, library was PCR amplified (15 cycles). Fragments were size-selected to eliminate adapter dimers contamination using AMPure XP beads. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on HiSeq3000 (RELACS ChIP-Seq, ATAC-Seq) and NextSeq HIGH (RNA-Seq) following manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4005179