title: GSM1533524: frg1 frg2 replicate 1 mRNA; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated with TRIzol (Ambion) from 0.1 g of tissue following the product manual. 2 µg of total RNA were treated with DNase I, amplification grade (Invitrogen) for 15 min at room temperature. DNase I was heat inactivated with 2.5 mM EDTA at 65 ºC for 10 min. Individual libraries for each biological replicate were generated from 2 µg total RNA according to the manufacturer’s instructions (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM1533524