title: Elevated heterozygosity in adults relative to juveniles provides evidence of viability selection on eagles and falcons
design: We quantified DNA concentration using a Qubit fluorometer and pooled equal amounts of DNA from each individual of each species x cohort (nestling or adult) x sex group (male or female).  Each of these twelve pools were used to prepare a PoolSeq library following the Illumina TruSeq DNA PCR-Free Sample Preparation Guide.  Covaris AFA Technology was used to generate ~400 bp insert sizes.  The four libraries associated with each species (i.e., adult male, adult female, juvenile male, juvenile female) were sequenced together on a single lane using an Illumina HiSeq2500.