title: GSM1632595: IFNg RPF replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: For RNAseq total RNA was isolated via Trizol extraction; for RPF cell lysates were treated with micrococccal nuclease, and momosomes were purified by sucrose gradient followed by sucrose cushion; RPFs were extracted by Trizol RPF and RNA libraries were prepared for sequencing using modified Illumina protocols with polyA selection (see online methods).
attributes.tag: GEO Accession
attributes.value: GSM1632595