title: GSM2420089: P4_Photoreceptor precursor_CD73+ RNA-Seq, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Retinal tissues (P4) were dissociated from eye balls of EGFP-BLIMP1 mice and the incubation with 0.1 % trypsin and 1 mM EDTA in PBS at 37 °C for 10 min.  Trypsin was then inactivated by adding equal volume of PBS/10% FBS supplemented with 0.1mg/mL DNaseI (Sigma). The cell suspension was stained with rat anti-CD73 (ecto-5`-nucleotidase) antibody conjugated with APC (BioLegend) . CD73 positive cells were purified with FACS. Total RNAs from collected cells were extracted with RNeasy Micro Kit (QIAGEN, 74004) according to the manufacture's instruction. RNA-seq libraries were prepared according to Nakamura et al., 2015, GEO:  GSE63266.
attributes.tag: GEO Accession
attributes.value: GSM2420089