title: GSM4191744: 69947_Igh VDJ-Seq Pro-B cell Igh(+/+); Mus musculus; OTHER
library_construction_protocol: Ex-vivo FACS sorted from bone marrow DNA was sheared using the Bioruptor sonicator (Diagenode) and subjected to end-repair and A-tailing, followed by ligation of adapters containing 12 UMI sequences using the NEBNext Ultra II DNA library prep kit for Illumina (NEB). A primer extension step with biotinylated JH-specific primers generated the single-stranded DNA products that were captured using Dynabeads MyOne streptavidin T1 beads (Thermo Fisher Scientific) and PCR-amplified with nested JH-specific and adapter-binding primers. The llumina sequencing adapter primers including the indexes for multiplexing of libraries were added to the PCR products in a final PCR amplification step.
attributes.tag: GEO Accession
attributes.value: GSM4191744