title: GSM2419866: IMR90-4 R1; Homo sapiens; OTHER
library_construction_protocol: IMR90, N and S-type DNA were purchased from the European Collection of Cell Cultures (ECACC), UK (https://www.phe-culturecollections.org.uk/); DNA from post mortem human brain (the prefrontal brain cortex, Brodmann area 10) of 50-year old healthy men was kindly provided by A.Petronis lab (CAMH, Toronto). DNA was extracted with phenol-chloroform extraction method. Libraries were constructed according to the TOP-seq (Tethered Oligonucleotide-Primed sequencing) protocol (Stasevskij et al., under revision). Briefly, sonicated genomic DNA was mTAG-labeled (Kriukiene et al., 2013) at unmodified CG sites, than  azide-tagged DNA was end-filled, a 3’-dA mononucleotide extension was added and DNA was ligated to  adapters. Ligated DNA was then subjected to Cu(I)-catalyzed cycloaddition reaction in which the priming oligodeoxyribonucleotide was attached to the azide moiety of the modified DNA. The TOP DNA synthesis was primed from the attached priming oligodeoxyribonucleotide, the products were amplified and subjected to Ion Proton sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2419866