title: GSM3560279: PCOS-IR miRNA; Homo sapiens; miRNA-Seq
library_construction_protocol: The small RNA and mRNA sequencing of cumulus granulosa cells from PCOS-IR and PCOS-control patients were performed at KangCheng-Shanghai. For miRNA sequencing, total RNA were extracted using TRIzol reagent (Invitrogen) and then pooled equally and then dispersed by 15% (w/v) denaturing PAGE.The small RNA fragments of about 18–28 nt were isolated by gel extraction and performed RNA purification. After small RNA library construction, these RNAs were sequentially sequenced by the Illumina NextSeq 500 (Illumina, San Diego, CA, USA) according to Illumina's protocol. For mRNA sequencing, total RNA were extracted and analyized by NanoDrop ND-1000. The mRNA enriched from total RNA was using NEB Next® Poly(A) mRNA Magnetic Isolation Module and then performed mRNA library construction by KAPA Stranded RNA-Seq Library Prep Kit (Illumina). The mRNA sequencing was also using the Illumina NextSeq 500 (Illumina, San Diego, CA, USA) according to Illumina's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3560279