title: Illumina HiSeq 2000 sequencing; Transcriptional Profiling of human pluripotent stem cells and and derived tissues
design: Transcriptional Profiling of human pluripotent stem cells and and derived tissues
library_construction_protocol: H9 embryonic stem cells were differentiated into definitive endoderm and then treated with FGF4/Chiron (a Wnt agonist) in order to generate intestinal organoids as previously described by  Spence, et al. Nature 470: 105-109 (2010) and McCracken, et al. Nature Protocols 6:1920-1928 (2011). RNA was extracted from samples and then prepared for RNAseq using the Illumina TruSeq mRNA method
attributes.tag: Experimental Factor: cell type
attributes.value: human intestinal organoids derived from H9 stem cells
attributes.tag: LIBRARY_STRAND
attributes.value: not applicable