title: Maize:TD:T66:400:L2:R1
design: Seeds of Zea mays cv. White Crystal, a glutinous maize cultivar, were purchased from a local supplier. For germination, seeds were imbibed in distilled water at 6:00 PM, shaken for 10 min at 200 rpm and then germinated on wet filter paper on Petri dishes in the dark room at 30 C and with 60% humidity. Plumules were collected every 6 h under dim green light in the dark room, the coleoptiles were removed within an hour and the embryonic leaves were frozen in liquid nitrogen and stored at -80 C.  Total RNA was extracted from isolated tissues using Trizol reagent (Invitrogen). To remove traces of DNA contamination, 1  L TURBO DNase (Ambion) per 10  g RNA was added and the reaction was incubated for 30 min at 37 , followed by phenol:chloroform extraction. The quality of extracted RNA was examined by gel electrophoresis and by BioAnalyzer (Agilent). RNA-seq libraries were prepared as in Liu et al. (2013) and were subjected to Illumina sequencing by paired-end 2*101nt format on a HiSeq2000 at the NGS Core Facility of Biodiversity Research Center in Academia Sinica, Taiwan.