title: Illumina HiSeq 2500 paired end sequencing; RNAseq of human cell line RPCI-WM1 comparing artificial PRDM1-targeting miRNA to non-targeting control, and comparing Tazemetostat treatment to DMSO. ChIPseq for the factors BLIMP1 and H3K27me3 in the RPCI-WM1, OPM-2 and NCI-H929 cell lines, along with ChIPseq for EZH2 in the NCI-H929 cell line.
design: RNAseq of human cell line RPCI-WM1 comparing artificial PRDM1-targeting miRNA to non-targeting control, and comparing Tazemetostat treatment to DMSO. ChIPseq for the factors BLIMP1 and H3K27me3 in the RPCI-WM1, OPM-2 and NCI-H929 cell lines, along with ChIPseq for EZH2 in the NCI-H929 cell line.
library_construction_protocol: Chromatin immunoprecipitation was performed on formaldehyde-crosslinked cells. ChIP DNA was extracted with phenol-chloroform. Sequencing libraries were prepared from ChIP DNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (#E7370S, New England Biolabs) with the Multiplex Oligos for Illumina (#E7335S and #E7500S, New England Biolabs).
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: input DNA