title: APY1 OE, biological replicate 2
design: RNA-Seq sample processing was carried out by the Genomic Sequencing and Analysis Facility (GSAF) of the University of Texas at Austin. RNA quality was confirmed by Agilent Bioanalyzer 2100 analyses of total RNA and poly(A)-selected mRNA using the Poly(A)Purist kit (Thermo Fisher).  cDNA libraries were prepared for sense strand-specific sequencing employing the NEBNext Ultra II DNA library kit. cDNA second strand synthesized with dUTP was degraded with uracil N-glycosylase, followed by PCR amplification with Illumina primers and ligation of adapters for the Illumina sequencing workflow.