title: GSM3755628: 11GGCTACL007SAll.fq; Mus musculus; RNA-Seq
library_construction_protocol: Hippocampi were immediately isolated, and the ventral third of the right hippocampus was snap frozen in liquid nitrogen. Tissue was lysed in lysis buffer using a probe sonicator. Total RNA was isolated using the RNeasy Lipid Tissue Mini Kit, Cat No: 74804 (QIAGEN) according to the manufacturer's instructions. Isolated RNA concentration and quality were determined by Qubit® quantitation assay using Qubit® 2.0 fluorometer (Invitrogen- life technologies, USA) and Agilent 4200 TapeStation System (Agilent Technologies, USA). Samples were prepared for Illumina sequencing using NEB's Ultra RNA Library Prep Kit for Illumina (NEB#7530) (BioLabs Inc, MA, USA) according to the manufacturer protocol. Total RNA was isolated using the RNeasy Lipid Tissue Mini Kit, Cat No: 74804 (QIAGEN) according to the manufacturer's instructions. Isolated RNA concentration and quality were determined by Qubit® quantitation assay using Qubit® 2.0 fluorometer (Invitrogen- life technologies, USA) and Agilent 4200 TapeStation System (Agilent Technologies, USA). Samples were prepared for Illumina sequencing using NEB's Ultra RNA Library Prep Kit for Illumina (NEB#7530) (BioLabs Inc, MA, USA) according to the manufacturer protocol.  Libraries were loaded at 8 PM and were sequenced with a 2 X 100 bp PE run on Illumina HiSeq 2500 using a V3 flow cell.
attributes.tag: GEO Accession
attributes.value: GSM3755628