title: GSM3172904: SMH_g3_2; Botryllus schlosseri; RNA-Seq
library_construction_protocol: Total RNA was extracted with Nucleo Spin RNA II kit from MN The standard protocol for library construction began with 1 ug of total RNA.  Since the protocol utilized was based on polyA capture, RNA was visualized on a BIoRad Experion to insure RNA Quality Index values were greater than 8.  For library construction the illumina TruSeq v2 mRNA kit was utilized.  The protocol was followed according to manufacturer's instructions except the final number of PCR cycles was 12 and not 15.  Following construction, libraries were visualized by Bioanalyzer (Agilent) using the High Sensitivity Chip and quantified for pooling and sequencing using Kapa Biosystems qPCR quantitation kit according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3172904