title: Illumina 16S amplicon sequence of bacteria on maple seedling roots
design: We amplified the V5-V6 region of the bacterial 16S ribosomal RNA                 gene using the chloroplast-excluding primers 799F-1115R. All amplified PCR samples                 were cleaned and normalized using the Invitrogen Sequalprep PCR Cleanup and                 Normalization Kit. DNA library was sequenced using an Illumina MiSeq Sequencer with                 the 2 300 bp paired end platform using the Illunima MiSeq Reagent Kit                 v3.