title: GSM4190702: Parent_CPT4h [CEM_4_H3K27Ac_S6]; Homo sapiens; ChIP-Seq
library_construction_protocol: Twenty-five million CCRF-CEM parental and SLFN11-KO cells were treated or untreated with CPT (100 nM) for 4 hours. ChIP assay was done by following the instruction manual of ChIP-IT Express (Active Motif). Briefly, cells were fixed with 1% formaldehyde in medium for 10 min at room temperature. Fixation was stopped with x1 glycine/PBS. Cells were lysed with lysis buffer with proteinase inhibitor cocktail and PMSF, and homogenized 60 times by small tight homogenizer. Cell pellets were re-suspended with sharing buffer, and sonicated 30 minutes by the following settings: 30 s on, 30 s off, level at H,  (Bioruptor XL). Five %/volume of each sample was saved as input. The left of the supernatant was incubated with 2 g antibody (H3K27ac or normal rabbit IgG) and protein G beads for overnight at 4°C. After reversing cross-link and proteinase K treatment, immunoprecipitated DNA was purified with ChIP DNA Clean & concentrator-5 (ZYMO Research) according to the manual.
attributes.tag: GEO Accession
attributes.value: GSM4190702