title: GSM4437361: HeLa_siZCCHC8_rep3_SLICCAGE; Homo sapiens; OTHER
library_construction_protocol: SLIC-CAGE preparation was performed as described in [47] with an input of 2000 ng of total RNA as starting material. Individually prepared SLIC-CAGE libraries with unique barcodes were pooled (8 per lane). The following 8 barcodes were used: # 1 (ACC), #2 (CAC), # 3 (AGT), # 4 (GCG), # 5 (ATG), # 6 (TAC), # 7 (ACG) and #8 (GCT). All used primers and adaptors were purchased from Integrated DNA technologies (IDT). RNA was isolated using TRIzol (Invitrogen) according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4437361