title: Illumina HiSeq 4000 paired end sequencing; Effects on adult testis of Sertoli cell-specific ablation of miR-17-92 in mouse embryos
design: Effects on adult testis of Sertoli cell-specific ablation of miR-17-92 in mouse embryos
library_construction_protocol: Amh-Cre and miR17-92flox mice were obtained form the Jackson Laboratory (Bar Harbor, ME, USA; Stock No: 007915 and 008458, respectively). They were bred in our animal house facilities and the resulting double heterozygous offspring carrying the Cre allele was backcrossed to homozygous miR-17-92flox mice to obtain  Amh-Cre;miR17-92flox/flox mice Mice were bred in our animal house facility at the Center of Biomedical Reseach of the University of Granada. Total RNA was isolated from three P60 Amh-Cre;miR17-92flox/flox and three control (miR17-92flox/flox) testes using the Quiagen RNeasy Midi kit following the manufacturer's instructions. Subsequently, the six samples were sent to Macrogen Inc. (Seoul, South Korea). After passing a quality check, libraries were prepared using TrueSeq RNA Sample Prep Kit V2 and they were paired-end sequenced separately in an Illumina HiSeq 4000 platform After successfully passing Macrogen Inc. quality check, libraries were prepared using TrueSeq RNA Sample Prep Kit V2 the six RNAs were paired-end sequenced
attributes.tag: Experimental Factor: phenotype
attributes.value: Amh driven deletion of miR17-92