title: RNA-seq of hulless barley Dulihuang mixed tissues
design: Six different tissues and developmental stages of hulless barley cv. Dulihuang were selected for RNA-seq and digital gene expression (DGE) analyses. The tissues included roots (Ro) and leaves (Le) from seven-day old seedlings, stems from the jointing stage (St), anthers harboring tricellular pollen (An), un-pollinated mature ovaries (Ov), and milky seeds (Ms). For each tissue sample, total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen).The six RNA samples and one mixed RNA sample from the six tissues were sent to BGI (Beijing Genomics Institute, Shenzhen, China) for RNA-seq (Illumina HiSeq 2000)and DEG sequencing analyses respectively.