title: GSM2151590: Th0 72h aIL4/4R H3K27Ac ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: RNA was harvested using BioRad total RNA extraction kit with DNaseI treatment. For ChIP cells were fixed with 0.8% formaldehyde for 8 min at RT and DNA was processed and sonicated according to the protocol from Covaris using Covaris S220 sonicator for 4-5 min. RNA  libraries for pair-end read sequencing were prepared by using standard Illumina protocols. ChIP libraries were prepared by using ThruPLEX DNA kit (Rubicon).
attributes.tag: GEO Accession
attributes.value: GSM2151590