title: GSM4505267: 452_KO_VtB6_neg_Str; Mus musculus; RNA-Seq
library_construction_protocol: mRNA was extracted from the analyzed tissues using a miRNeasy Kit (Qiagen) according to the manufacturer's instructions. All mRNA samples were examined for quantity and quality by a Bioanalyzer (Agilent). Total RNAs with an average RIN value of 8.52 ± 0.05 were used for RNA-seq library preparation. mRNA was purified from 2 μg of total RNA by NEXTflex poly(A) beads (Bioo Scientific, 512981), subjected to fragmentation and first and second strand syntheses, and cleaned up by EpiNext beads (EpiGentek, P1063). The second strand DNA was adenylated, ligated, and cleaned up twice by EpiNext beads. cDNA libraries were amplified by PCR and cleaned up twice by EpiNext beads. cDNA library quality was quantified by a 2100 Bioanalyzer using an Agilent high-sensitivity DNA kit (Agilent, 5067-4626). Libraries were sequenced separately as 75-bp single ends on an Illumina NextSeq 500. The sequencing was performed at the McDermott Sequencing Core at UTSW.
attributes.tag: GEO Accession
attributes.value: GSM4505267