title: ITS rDNA-Seq: forest soil
design: The total community DNA was extracted from soils by use of a MoBio                 Power Soil DNA extraction kit. The ITS region was amplified in triplicate using the                 barcoded ITS1F and 2043R primer set. PCR products were quantified, purified and                 sequenced by Illumina Miseq platform for assessment of fungal diversity and                 community structure.