title: small RNA 18-30 (nucleotide) cloned from FLAG(Ago3) Immunoprecipitation from nanos/Gal4  driven FLAG-Myc-Ago3[ADH] ovary
design: 0-2 days old w1 flies were collected and fed with yeast. Ovaries were dissected after 3 days. Lysate and immunoprecipitation was carried out as described[1].  Reference 1. Zhang Z., Xu J., Koppetsch B.S., Wang J., Tipping C., Ma S., Weng Z., Theurkauf W.E., and Zamore P.D. (2011). Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains. Mol Cell. 44, 572-584.