title: UCE target enrichment of Leptanilla_revelierii_D0412_CCAACTTC+CCTCGTTA (CASENT0006788) [raw reads]
design: DNA was extracted non-destructively using a DNeasy Blood and Tissue Kit (Qiagen Inc., Valencia, CA) according to manufacturer instructions. DNA was quantified for each sample with a Qubit 2.0 fluorometer (Life Technologies Inc., Carlsbad, CA). Phylogenomic data were obtained from these taxa using the hym-v2 probe set, with libraries being prepared and target loci enriched using the protocol of Branstetter et al. (2017). Enrichment success and size-adjusted DNA concentrations of pools were assessed using the SYBR FAST qPCR kit (Kapa Biosystems, Wilmington, MA) and all pools were combined into an equimolar final pool. The contents of this final pool were sequenced by an Illumina HiSeq 2500 at the University of Utah's High Throughput Genomics Facility. For trimming raw reads, use i7:GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*ATCTCGTATGCCGTCTTCTGCTTG and i5:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT*GTGTAGATCTCGGTGGTCGCCGTATCATT, where * indicates the index sequence.