title: ITS2 profiling of patina metagenome
design: ITS2 fungal region (amplified using the following primers: ITS3 PCR Forward Primer 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-GCATCGATGAAGAACGCAGC-3 and ITS4 Reverse Primer 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-TCCTCCGCTTATTGATATGC-3)  were subject to amplicon library preparation (amplified using the primers described in Illumina 16s protocol:  15044223 Rev. B)