title: GSM1634797: Jun_4C_BMDMs_UT; Mus musculus; OTHER
library_construction_protocol: 4C templates were prepared as previously described (Splinter et al., 2012).  Briefly, chromatin was cross-linked with 2% formaldehyde for 10 min at room temperature, nuclei were isolated and cross-linked DNA was digested with the 4bp cutter DpnII. After proximal fragments ligation, cross-links were reverted. The second digestion was performed with the 4bp cutter Csp61 and followed again by intramolecularligation. 200 ng of the obtained 4C template were used to perform 8 individual PCR reactions, which were then pooled together and purified for HT sequencing. Adaptor sequences necessary for Illumina single-read sequencing (Hi-seq 2000) were added to 4C primer pairs as additional 5′ overhangs
attributes.tag: GEO Accession
attributes.value: GSM1634797