title: GSM2340976: TAµ_BU_TX [Sample_c2]; Mus musculus; RNA-Seq
library_construction_protocol: RNeasy Plus Micro kit (Qiagen) was used to extract RNA from the sorted myeloid brain populations. In particular we retrieved different myeloid brain subpopulation sorted from: P10 (TAµ cells), 5 month old C57bl6/j mice (ADULT µ cells) and BU-treated mice(BU_TX) at 3 months from GFP Lin- transplantation (µ and TAµ cells). RNeasy Plus Micro kit (Qiagen) was used to extract RNA from the sorted myeloid brain populations Briefly, amplification of cDNA from total RNA (starting amount =100 ng per sample) was performed using the Ovation RNA-Seq System V2 (Nugen), cDNA was then fragmented and ligated into a sequencing library using NuGEN's Ovation Ultralow Library Systems After barcoding, the RNA libraries were pooled, denatured and diluted to an 8 pM final concentration. Cluster formation was performed on cBot (Illumina) (single-end) using flow cells v.3. The SBS (sequencing by synthesis) was performed according to TruSeq SR protocol (Illumina) for the HiSeq 2500 (Illumina) set to 50 cycles, yielding an average of 30x106 clusters for each sample
attributes.tag: GEO Accession
attributes.value: GSM2340976