title: GSM2759483: CLIPseq_Pspc1; Mus musculus; RIP-Seq
library_construction_protocol: Crosslinking immunoprecipitation and massive parallel sequencing (CLIP-seq) was performed as previously described (Mol Cell. 2009 Dec 25;36(6):996-1006. doi: 10.1016/j.molcel.2009.12.003.) by using a FLAG tag antibody (Sigma #F1804) targeting Pspc1. CLIPed RNA was extracted with TRIzol and ligated with 5' RNA linker GUUCAGAGUUCUACAGUCCGACGAUC for 16 h at 16°C. Reverse transcription with RT primer AGACGTGTGCTCTTCCGATCT, followed by PCR for 25 cycles to generate and amplify the cDNA library. High-throughput sequencing of the resulting cDNA was performed on a Hiseq-2000 sequencer according to the manufacturer’s protocol.
attributes.tag: GEO Accession
attributes.value: GSM2759483