title: RNA-Seq of Cavia porcellus: adult male adrenal gland
design: RNA was isolated using the RNeasy Mini kit (Qiagen). The RNA was treated with DNase I on the affinity column before elution. Strand-specific RNA-seq libraries, including poly-A(+) mRNA selection and RNA fragmentation, were prepared using the TruSeq Stranded RNA LT Kit (Illumina). Ten libraries were combined into a single pool for sequencing.