title: Illumina HiSeq 2500 paired end sequencing; ChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
design: ChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
library_construction_protocol: Splenocytes were isolated and sorted for CD3 CD19 CD45 CD11c MHC-II to high purity. Fixed chromatin from isolated cells was sheared and precipitated for H3K4me3 and H3K27me3 Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany
attributes.tag: Experimental Factor: C57BL/6
attributes.value: strain
attributes.tag: Experimental Factor: IFNAR1 knockout
attributes.value: genotype
attributes.tag: Experimental Factor: H3K27me3
attributes.value: immunoprecipitate