title: GSM2769417: dHIP_TAU CSP_H2Bac_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP-seq libraries were prepared using NEXTflex ChIPSeq Kit (#5143-02, Bioo Scientific) following the manufacturer’s protocol (V12.10) with some modifications. Briefly, 10 ng of ChIP-enriched DNA or Input DNA were end repaired using T4 DNA polymerase, Klenow DNA polymerase and T4 PNK, then size selected and cleaned-up using Agencourt AMPure XP beads (#A63881, Beckman). A single ‘A’ nucleotide was added to the 3′ ends of the blunt DNA fragments with a Klenow fragment (3′ to 5′exo minus). The ends of the DNA fragments were ligated to double-stranded barcoded DNA adapters (NEXTflex ChIP-Seq Barcodes-6, #514120, Bioo Scientific) using T4 DNA Ligase. The ligated products were enriched by PCR [2 min at 98°C; (30 s at 98°C, 30 s at 65°C, 60 s at 72°C) x 14 cycles; 4 min at 72°C] and cleanedup using Agencourt AMPure XP beads.
attributes.tag: GEO Accession
attributes.value: GSM2769417