title: WGS of Pseudomonas
design: DNA samples were sheared into 400-500 bp fragments using a Covaris M220 Focused-ultrasonicatorTM (ThermoFisher Scientific, Waltham, Massachusetts, USA) according to manufacturers protocol. Illumina sequencing libraries were prepared from the sheared fragments using the NEXTflex Rapid DNA-Seq Kit (Bioo Scientific, Austin, TX, USA). Briefly, 5 prime ends were first end-repaired and phosphorylated. Next, the 3 ends were A-tailed and ligated to sequencing adapters. The third step is to enrich the adapters-ligated products using PCR. The prepared libraries were then used for paired-end Illumina sequencing (2  150 bp) on an Illumina HiSeq X Ten machine (Illumina, San Diego, CA, USA).