title: GSM4192210: LTF_40_RFD; Mus musculus; RNA-Seq
library_construction_protocol: For analyses of gene expression, RNA was extracted from cell suspensions of small intestinal tuft cells (EpCAM+CD45+ Siglec-F+). Total RNA concentration was measured, checked for quality and used for cDNA preparation by reverse transcription of 5.6 μL of purified RNA. cDNA was amplified using Smart-seq2 protocol (Picelli et al., Natue protocols 2014), purified, quantified, and checked for quality and library size. Nextera XT DNA library preparation kit  (Illumina, FC-131-1096) was used with 1 ng purified cDNA for the construction of sequencing libraries. cDNA libraries having insert sizes of 500-800 bp were used for paired-end sequencing on the Illumina NovaSeq 6000 sequencing platform (Novogene Corporation, China).
attributes.tag: GEO Accession
attributes.value: GSM4192210