title: GSM2759023: HeLa_Control_AMO_8hr_IgG_ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were harvested and then crosslinked with 1% Formaldehyde. Nuclei were chemically isolated and then lysed, then sheared using a sonicator. The resulting sample was cleared using dynabeads, and polII-DNA complexes were isolated with the antibody. Libraries were prepared according to instructions accompanying the TruSeq ChIP sample preparation kit from Illumina (# IP-202-1012). Briefly, the overhanging DNA ends were blunted using exonuclease, and then the 3' end was adenylated to prevent improper ligation. The adapters were then ligated, and the library was amplified.
attributes.tag: GEO Accession
attributes.value: GSM2759023