title: HKU5 Coronavirus Transcriptome - Mus Musculus virulent
design: Goals: To define known and unknown virus-specific, virus-encoded transcripts present during infection with various coronaviruses. Setup and Design: Vero cells were infected with HKU5-SEcto-MA for 24 hours. Intracellular RNA was then harvested in TRIzol. RNA purified from TRIzol preps was subjected to RT-PCR and poly-A purification before generation of first - and second-strand cDNA and Illumina HiSeq analysis.