title: GSM2680245: IP_GFP-AGO1_rep1; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: For each GFP-AGO1 allele, two independent pools of ~10 different lines were frozen and ground in liquid nitrogen. Supernatants were precleared and incubated with GFP-Trap_MA (ChromoTek). The beads were removed magnetically and RNA was then phenol-extracted and precipitated in sodium acetate, ethanol, and glycogen, washed in ethanol 80% and re-suspended in RNase-free water. Extracted RNA was processed into sequencing libraries using adapted Illumina protocols and sequenced at Fasteris (http://www.fasteris.com, Switzerland) using the Illumina HiSeq sequencer
attributes.tag: GEO Accession
attributes.value: GSM2680245