title: GSM4435072: Control 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from control and treated cells using Trizol. 1ug of total RNA was used to enrich mRNA with NEBNext Poly(A) mRNA magnetic isolation Module (E7490, New England Biolabs, USA) as per the manufacturer's instructions. The enriched mRNA was used for library preparation. NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, New England Biolabs, USA) was used to prepare the library by following the manufacturer's instructions. The NEBNext Multiplex Oligos for Illumina (E7335S, New England Biolabs, USA) was used to perform polymerase chain reaction (PCR) to generate indexed sequencing libraries. The library quality and quantity were checked using Agilent Bioanalyzer 2100 with DNA 1000 kits and Qubit dsDNA HS Assay Kit.
attributes.tag: GEO Accession
attributes.value: GSM4435072