title: GSM3100897: ChIP_MT_DRRKD_Input_rep1; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIPSeq,cells were cross-linked in 1% formaldehyde and lysate to extract nuclei. The chromatin was sonicated to 250 bp fragments.  Lysates were cleared from sonicated nuclei and used for immunoprecipitation  For ChIRPseq, cells were cross-linked in 1% glutaraldehyde. Nuclei were sonicated and used for probe hybridization. For ATACseq, nucli were isolated and   treated with Tn5 transpoase. For ChIPSeq,  10ng of immuno-precipitated DNA fragments were used to prepare ChIP-seq libraries with NEBNext Ultra DNA library prep kit for Illumina (New England BioLabs) following the manufacturer's protocol.  NEBNext Ultra II RNA library prep kit (New England BioLabs) was used to process RNA-Seq libraries following the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM3100897