title: GSM4505866: Tumor T1 WGBS; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Frozen tissues of surgically removed specimens  were used for genomic DNA extraction.  Genomic DNA was extracted with QIAmp DNA Mini kits (Qiagen) from fresh frozen tissue samples. 1μg gDNA was fragmented by sonication with a base pair peak of 300 bp for the resulting fragment, and adaptors were then ligated to both ends of the fragments.  Bisulfite conversion was performed to whole genomes of ten pairs of ESCC and matched normal tissues, where converts cytosine residues of the dinucleotide CpG to uracil but leaves methylated cytosine unaffected. PCR amplification and purification were carried out. The uracil-binding pocket of KAPA HiFi DNA Polymerase has been inactivated, enabling amplification of uracil-containing DNA. The high quality of the library was estimated by The Qubit® 3.0 Fluorometer. Bisulfite conversion success ratio is 99.18% and 99.49%, respectively in normal and ESCC samples The WGBS library was sequenced on an Illumina HiSeq2500 sequencers and generated 400M of paired-end reads (2x125bp)
attributes.tag: GEO Accession
attributes.value: GSM4505866