title: RNA-seq of INS1 cells
design: Library Strategy: RNA-seq. Total RNA was extracted and purified from MIN6 and INS-1(832/13) beta cell lines using Trizol (Life Technologies) according to the manufacturer’s instructions, and sequenced using the Illumina TruSeq (human islets, MIN6) or Kapa Biosystems KAPA mRNA-seq  (832/13)  kits according to the manufacturer’s instructions.