title: Illumina HiSeq 2500 sequencing; small RNA-seq of Arabidopsis pol2a mutants
design: small RNA-seq of Arabidopsis pol2a mutants
library_construction_protocol: Immature inflorescences (stage 1-12) were used for the study. Plants were grown in soil in a growth cabinet at 23°C, 50% humidity, using long day conditions (16h light, 8h dark). Total RNA was extracted with guanidine hydrochloride (8 M guanidine hydrochloride, 20 mM MES (2-morpholinoethanesulfonic acid), 20 mM EDTA and 50 mM β-mercaptoethanol at pH 7.0). Total RNA purified from immature inflorescences was used to generate small RNA libraries (TruSeq small RNA; Illumina).
attributes.tag: Experimental Factor: genotype
attributes.value: L5