title: Solvent Methylcellulose and DMSO 1
design: gDNA was sheared, end-repaired, and "A" based at the 3' ends, then adapters were ligated to the "A" based DNA, and transposon-containing fragments were enriched by PCR followed by a second nested PCR to add the Illumina primer/capture sites for flow cell binding and the barcodes for multiplex sequencing.