title: GSM4628204: in1; Bos taurus; miRNA-Seq
library_construction_protocol: Mammary gland tissues were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. The RNA sample was preparated by using total of 2 µg RNA. Firstly, Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA) was used to remove ribosomal RNA, and ethanol precipitation was perfomed to clean the rRNA free residue. Subsequently, the rRNA-depleted RNA by NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) was used to generate sequencing cDNA libraries following manufacturer's recommendations. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4628204