title: experiment: second colonization: mouse colonized gat-negative E. coli, mouse 7, day 17 post-colonization
design: 16S rRNA gene amplification and sequencing was carried out at the Gene Expression Unit from Instituto Gulbenkian de Cincia, following the service protocol. For each sample, the V4 region of the 16S rRNA gene was amplified in triplicate, using the primer pair F515/R806, under the following PCR cycling conditions: 94źC for 3 min., 35 cycles of 94źC for 60s, 50źC for 60s and 72źC for 105s, with an extension step of 72źC for 10min (Caporaso et al. 2011; Caporaso et al. 2012). Samples were then pair-end sequenced on an Illumina MiSeq Benchtop Sequencer, following Illumina recommendations.