title: Illumina MiSeq sequencing; qiita_ptid_6079:12189.DIVSTATS3ITS
design: An incubation study using thermally altered pine needles in a pine plantation soil; soils were harvested at 43 days after exposure.
library_construction_protocol: Target aplicon: ITS. Image analysis was performed in real time by the MiSeq Control Software (MCS) v2.6.2.1and Real Time Analysis (RTA) v1.18.54, running on the instrument computer. RTAperforms real-time base calling on the MiSeq instrument computer. Then the Illuminabcl2fastq 2.20.0.422 pipeline was used to generate the sequence data. The data generatedhere meet the AGRF quality standards.
attributes.tag: barcode
attributes.value: GGACTCCTTCGACTAG
attributes.tag: center_name
attributes.value: AGRF
attributes.tag: flowcell_id
attributes.value: V1-V3
attributes.tag: lane
attributes.value: 1
attributes.tag: orig_name
attributes.value: DIVSTATS3-ITS
attributes.tag: pcr_primers
attributes.value: FWD: CTTGGTCATTTAGAGGAAGTAA; Rev: GCTGCGTTCTTCATCGATGC
attributes.tag: primer
attributes.value: CTTGGTCATTTAGAGGAAGTAA
attributes.tag: primer_forward
attributes.value: CTTGGTCATTTAGAGGAAGTAA
attributes.tag: primer_reverse
attributes.value: GCTGCGTTCTTCATCGATGC
attributes.tag: run_prefix
attributes.value: DIVSTATS3ITS
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: target_gene
attributes.value: ITS
attributes.tag: target_subfragment
attributes.value: ITS1/2