title: D_sechellia_R1
design: Please see Garrigan et al. 2012. Genomic DNA (gDNA) was extracted from 15 female flies. gDNA was also extracted from 15 female flies of the D. sechellia reference strain (14021-0248.25). gDNA was extracted using a modified Puregene DNA Extraction Protocol. The 15 frozen female flies for each library were lysed by 300 L cold Cell Lysis Solution (Qiagen, 158906) with pestle homogenization and incubation at room temperature for 15 min, and treated with 4.5 L RNase A at 37C for 15 min. Proteins were precipitated out with 100 L Protein Precipitation Solution (Qiagen, 158910) and centrifugation at 16,000g for 4 min. gDNA in supernatant was purified by DNA Clean and Concentrator 25 Kit (Zymo, D4005) with five volumes of binding buffer to sample. For the library preparations of  D. sechellia, 3 g gDNA was sheared to 300 bp with Covaris, followed with end-repairing, A-tailing, ligating to adaptors, selecting size between 300 and 450 bp with 2% agarose gel, and PCR amplifying for 15 cycles. The final product size was estimated to be between 400 and 500 bp on a 2% agarose gel.