title: Illumina Genome Analyzer IIx sequencing; Rna-seq of control and 2mM NaBu-treated zebrafish larvae (8 dpf)
design: Rna-seq of control and 2mM NaBu-treated zebrafish larvae (8 dpf)
library_construction_protocol: zebrafish were isolated during pharyngula stage, daily fed and water-changed until 8 dpf 2 mM NaBu for 24 hours in some samples Whole larvae where immersed in RNA later, and RNA was extracted using a Qiagen kit following manufacturer recommendations. RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit v2 from Illumina (RS-122-2001)
attributes.tag: Experimental Factor: compound
attributes.value: PBS