title: GSM4433455: KO2_H3K27ac; Mus musculus; ChIP-Seq
library_construction_protocol: BMDMs were derived as previously described (Trouplin et al., 2013) from Copwt/wt Rosa26-CreERT2 and Copfl/fl Rosa26-CreERT2 mice. Cop1 deletion was achieved by treatment with 4-OHT (0.1 µM) for 48 h in culture. Activ Motif Epigenetic services performed ChIP assays. Cells fixed with 1% formaldehyde for 11 min were quenched with 0.25 M glycine and then were swollen in lysis buffer for 30 min on ice. Chromatin was isolated after sonication to shear the DNA to an average length of 300–500 bp. Input and immunoprecipitated DNAs were treated with RNase, proteinase K, and heat for de-crosslinking, followed by ethanol precipitation. Antibodies used for immunoprecipitation were c/EBPß (Santa Cruz H-7), H3K4me3 (Abcam ab8580) and H2K27ac (Abcam ab4729). Libraries were prepared using standard protocols for the Ovation Ultralow System. Briefly, samples were PCR-amplified and sized using the Agilent 2100Bioanalyzer. Barcoded libraries were sequenced 75 bp single end on HiSeq 2500 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4433455