title: Whole Genome Sequencing of Egyptian individual
design: Genomic DNA was extracted from blood with the Gene JET Blood genomic DNA purification Kit (Thermo Scientific, USA), following the manufacturerĄŻs protocol. A library of 400-500 bp insert size was constructed. Genomic DNA was sheared using Covaris S series (Covaris, MS, USA). The sheared DNA was end-repaired A-tailed, and ligated to paired-end adapters, according to the manufacturerĄŻs protocol (Truseq DNA Sample Prep Kit v2, Illumina, San Diego, CA, USA). Adapter-ligated fragments were then size selected on a 2% Agarose gel, with the 520-620 bp band being extracted. The ligated DNA fragments which contained adapter sequences were enhanced via PCR using adapter specific primers. Library quality and concentration were determined using theAgilent 2100 BioAnalyzer. The libraries were quantified using a KAPA library quantification kit (KapaBiosystems, MA, USA), according to IlluminaĄŻs library quantification protocol.