title: GSM2418417: Nonstressed female 3 (NSF3) mRNA; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq libraries were generated from large RNA fractions (>200 bp) with RIN values > 8.8. RNA (1 ug) was used for mRNA library construction according to the standard protocol of the TruSeq RNA Library Preparation Kit v2 (Illumina, San Diego, CA). 14-gauge ventral striatum punches were collected from each animal and flash frozen on dry ice. RNA was isolated using homogenization in Qiazol (Qiagen, Hilden, Germany) followed by chloroform layer separation. Bilateral NAc punches from 5 animals (10 NAc punches) were pooled for each library at the point of homogenization. The aqueous RNA layer was further processed via miRNeasy mini kit (Qiagen, Hilden, Germany) to yield separate fractions enriched for small RNA (<200 bp; for small RNA sequencing) and large RNA (>200 bp; for mRNA sequencing).
attributes.tag: GEO Accession
attributes.value: GSM2418417