title: GSM3325431: WT_3; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: RNA was extracted from cell pellets using an acid phenol procedure followed by pelleting in CsCl2, resuspension, ethanol precipitation, and purification by Qiagen Rneasy mini kit. Sequencing libraries were constructed using NEBNext® Multiplex Oligos (Index Primers Set 1 and Set 2) and NEBNext® ChIP-seq Library Prep Reagent Set for Illumina, and subjected to 50 bp single-end sequencing in an Illumina Hiseq2500 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM3325431