title: mRNA sequencing of Perkinsus olseni 00978-12
design: Total RNA was extracted from isolate 00978-12 using the RNeasy Mini Kit (QIAGEN) utilizing bead-assisted lysis performed with a TissueLyser II (QIAGEN). Sequencing libraries were generated using the TruSeq stranded mRNA kit (Illumina) with sequencing performed using a NextSeq500 utilizing mid output 2x75 bp paried-end reads. Transcriptome sequencing of 00978-12 was performed at the Ramaciotti Centre for Genomics (UNSW, Australia)