title: Illumina HiSeq 2000 sequencing; Variation in RNA expression in a panel of 30 breast cancer cell lines
design: Variation in RNA expression in a panel of 30 breast cancer cell lines
library_construction_protocol: Cells were grown for 48 hours in 60 mm dishes, in their respective medium, at subconfluent density. Total RNA was extracted using TRIzol reagent (15596-018, Ambion life technologies) according to the manufactures protocol. Illumina TruSeq mRNA libraries were generated using the TruSeq RNA Library Preparation Kit v2 sample preparation kit (Illumina Inc., San Diego, Cat.No RS-122-2001/2) according to the manufacturer's instruction (Part # 15026495 Rev. B)
attributes.tag: Experimental Factor: cell line
attributes.value: MDAMB436