title: Amplicon seq of mosquito larva 6: carcass site 10
design: Total DNA was extracted using DNeasy PowerSoil Kit. Extracted DNA was quantitated using NanoDropTM 2000/2000c spectrophotometers (Thermo Scientific, Waltham, MA, USA) and standardized at a concentration of 2 ng/L. The fungal amplicon library was generated by triplicate PCR amplification using barcoded primers fITS7 and ITS4. PCR product purification was performed with Mag-Bind RXNPure plus (Omega Bio-tek; Norcross, GA, USA). Cleaned PCR products were pooled at equal concentrations for a final sequencing library concentration of 1.5 g.