title: GSM3324730: s4U RNA with TimeLapse chemistry rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Metabolically labeled and control cells were collected by centrifugation (3 min, 1000 g, 4C) and lysed in TRIzol (Life Technologies) and RNA was extracted according to Duffy E. E. and Simon M. D. 2016. Extracted RNA was treated with Turbo DNase (Invitrogen) for 1h at 37C, extracted with phenol/chloroform and precipitated as before. Total RNA was treated with TimeLapse chemistry according to Schofield et al. 2018. Libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input V2 Mammalian (Takara Bio USA, cat. 635005). For targeted sequencing 3 amplicons of ACTB were reverse transcribed and libraries were prepared according to Sexton et al. 2017.
attributes.tag: GEO Accession
attributes.value: GSM3324730