title: WGS of Sockeye Salmon: Pitt Lake Gynogen Fin
design: Genomic DNA was sheared using a Covaris g-TUBES (Covaris Inc.) at 4000 RPM for 60 seconds on each side on an Eppendorf centrifuge model 5424.  Template was prepared according to Pacific Biosciences 20 kb Template Preparation with the SMRTbell Template Prep Kit 1.0 reagents (Pacific Biosciences) with a template range of 9-50 kbp.  Libraries were sequenced with a loading concentration of 15 pM (sequencing primer v3, 0.83 nM and Sequel 2.1 polymerase 0.5 nM, Sequel Sequencing Plate 2.1, SMRT cells 1M v2 and 10 hours movies).