title: GSM2339914: mESC_DKOzero_Dnmt3AOE_RRBS; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was quantified using a Qubit® 2.0 Fluorometer, and quality-assessed on an Agilient 2200 TapeStation D1000 ScreenTape.  RRBS was performed on 20ng of each sample using the NuGen Ovation® RRBS Methyl-Seq System.  Barcoded samples were pooled in groups of 8 immediately prior to Bisulfite Conversion with the Qiagen EpiTect® Fast Bisulfite Conversion kit.  Library pools were purified with a 1X Agencourt RNA XP bead clean-up and sequenced on an Illumina HiSeq® 2500 Sequencing System. All protocols were carried out according to manufacturer's recommendations except where specified. genomic DNA was purified using Phenol/Chloroform extraction
attributes.tag: GEO Accession
attributes.value: GSM2339914