title: GSM3324270: DN4 population; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were extracted from suspensions of thymus of six pooled C57BL/6 female mice DN2, DN3, and DN4 cells were sorted by flow cytometry (using a FACSAria IIu, BD Biosciences). Double-negative cells were gated for CD4-, CD8-, CD3- and B220- expression. DN2 cells were additonally gated for CD25high, CD44high and CD177high expression, while DN3 cells were gated for CD25high, CD44low and CD117low expression, and DN4 cells were gated for CD25-, CD44-, CD117- and CD28+ expression. Around 5,000 cells from the DN2 and DN4 populations were loaded on one well each, along with around 10,000 cells from the DN3 population loaded on two wells of a 10× Genomics Chromium Single Cell Controller. Single-cell capture and cDNA and library preparation were performed with a Single Cell 3' v2 Reagent Kit (10× Genomics) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3324270