title: sRNA-seq of MARC-145 cells
design: A total of 107 MARC-145 cells were harvested in TRIzol reagent (Invitrogen) and total RNA was purified according to manufacturers instructions. Genomic DNA was removed using the RNase-Free DNase Set (QIAGEN). RNA was quantified using a nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific), and RNA quality was assessed by agarose gel electrophoresis. Small RNA sequencing was further performed using Illumina HiSeq2000 (Illumina; Huada Genomics Institute Co., Ltd., China).