title: RNA-Seq of Gossypium hirsutum 10 day post anthesis cotton fiber
design: "Illumina TruSeq RNA sample prep kit v2 was used. 1 ?g of RNA was purified         using poly-T oligo-attached magnetic beads. The mRNA was chemically fragmented using         divalent cations and fragments were reverse transcribed into first strand cDNA using random         primers. Second strand synthesis was carried out using DNA Polymerase I and RNaseH. Ends         were repaired on the double-stranded cDNA to convert overhangs into blunt ends, 3? ends were         adenylated, and adapters with indexes for multiplexing were ligated. The library was         amplified with a PCR reaction and then small fragments were removed with AmpureXP beads. The         final library was quantified on the Agilent Bioanalyzer High Sensitivity DNA         Chip"