title: RKO WT1
design: RNA was extracted from wild type and BCL9 knockout RKO cells with or without Poly I:C treatment (1g/ml) and subsequently purified using the TURBO DNA-free Kit (AM1907, invitrogen) to remove any residual DNA. An RNA library was prepared using the ribosome RNA removing method and sequenced with a 150bp paired-end protocol in the Center for Cancer Computational Biology at the Dana Farber Cancer Institute.