title: GSM4182287: Imp RIPseq 1; Drosophila melanogaster; RIP-Seq
library_construction_protocol: Brains were removed and flash frozen in liquid nitrogen before lysis. Imp::GFP was immunoprecipitated using anti-GFP agarose beads. RNA was harvested using RNASpin Mini kit (GE Healthcare). polyA RNA was enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) according to manufacturer's instructions. Poly(A) enriched RNA was then used for library production using the Ion Total RNA-Seq Kit v2 for Whole Transcriptome Libraries (Life Technologies). Libraries were prepared using Ion Total RNA-Seq Kit v2 for Whole Transcriptome Libraries (Life Technologies) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4182287