title: Illumina HiSeq 2000 paired end sequencing; ChIP-seq of PDAC tissues grown as patient-derived tumor xenografts.
design: ChIP-seq of PDAC tissues grown as patient-derived tumor xenografts.
library_construction_protocol: PDAC tissue from surgical samples was fragmented, mixed with 100 μL of Matrigel and implanted with a trocar (10 gauge; Innovative Research of America, Sarasota, FL) in the subcutaneous right upper flank of an anesthetized and disinfected male NMRI-nude mouse. Samples obtained from EUS-FNA were mixed with 100 μL of Matrigel (BD Biosciences, Franklin Lakes, NJ) and injected in the upper right flank of a male nude mice (Swiss Nude Mouse Crl: NU(lco)-Foxn1nu; Charles River Laboratories, Wilmington, MA) for the first implantation. When xenografts reached 1 cm3, these were removed and passed to NMRI-nude mice in the same manner as the surgical samples. All animal experiments were conducted in accordance with institutional guidelines and were approved by the “Plateforme de Stabulation et d'Expérimentation Animale” (PSEA, Scientific Park of Luminy, Marseille). None No treatment was provide DNA was extracted using Blood & Cell culture DNA mini kit (Qiagen) following the manufacturer's instructions.
attributes.tag: Experimental Factor: phenotype
attributes.value: classical
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: anti-H3K4me1