title: GSM3938472: TOP2B_SNB19_ETO_chip-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Nuclear extracts were isolated, chromatin fragmented using Covaris sonicator in 1mL tubes for 1min. 10 ug of antibody (TOP2A Abcam, TOP2B Abcam) was incubated overnight. Washes with RIPA Buffer 4 times and once of TE with 50mM NaCl. Reversed crosslink and DNA purified for sequencing using phenol:chloroform:isoamyl alcohol.
attributes.tag: GEO Accession
attributes.value: GSM3938472