title: GSM1417150: 0h; Danio rerio; miRNA-Seq
library_construction_protocol: Retinas were collected in TRIzol reagent and total RNA was extracted and size fractionated on 15% urea acrylamide gels. 15-30 nt small RNAs were size selected, ligated to 3’ and 5’ end adapters and amplified by RT-PCR to generate small RNA libraries as described (Wei et al., 2012). Libraries were constructed following the protocol published by Wei et al., 2012
attributes.tag: GEO Accession
attributes.value: GSM1417150