title: Illumina HiSeq 2500 paired end sequencing; Lrig2 is required for maturation of peripheral nerves in the urinary bladder
design: Lrig2 is required for maturation of peripheral nerves in the urinary bladder
library_construction_protocol: The mice are a targeted knockout (LoxP/Cre) of Lrig2 exon 12 from strain C57BL/6 at up to 24 hours of age. Bladders from mice aged one - two weeks were excised and washed in PBS then fixed whole or opened ventrally (to preserve the pelvic ganglia and trigone) and fixed flat between glass slides in 4 % paraformaldehyde (Sigma) at 4oC for 24 hours. Whole neonatal bladders were homogenized using Biopulveriser Lysing Matrix D beads on a Fast-Prep-24 (MP Biomedicals). RNA was isolated using the Qiagen RNeasy kit. Illumina TruSeq Stranded mRNA Library Prep Kit
attributes.tag: Experimental Factor: genotype
attributes.value: LRIG2 knockout