title: GSM3755755: XBP1 LKO CT2 RNA-Seq rep2; Mus musculus; RNA-Seq
library_construction_protocol: Mouse liver tissues were collected from XBP1Flox (n=2) and XBP1LKO (n=2) mice at 2h interval for a total of 48 hours under constant darkness condition. Total RNA was isolated from mouse liver with miRNeasy Mini Kit (Qiagen) per the manufacturer's instructions. Extracted RNA samples underwent quality control assessment using the RNA Pico 6000 chip on Bioanalyzer 2100 (Agilent) and were quantified with Qubit Fluorometer (Thermo Fisher). Strand-specific total mRNA-Seq libraries were prepared using the Universal Plus mRNA-Seq kit (NuGen) per the manufacturer's instructions using 200ng of RNA. The size selection for libraries were performed using SPRIselect beads (Beckman Coulter) and purity of the libraries were analyzed using the High Sensitivity DNA chip on Bioanalyzer 2100 (Agilent). The prepared libraries were pooled and sequenced using NoveSeq 6000 (Illumina), generating ~40 million 2×100 bp paired-end reads per samples. RNA-Seq library preparation and sequencing were performed at Univesity of Pittsburgh Medical Center (UPMC) Genome Center.
attributes.tag: GEO Accession
attributes.value: GSM3755755