title: GSM3755959: H2C KD cell line sh4 plate 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from small sections of primary tumor tissue using TriPure, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy® Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent for sequencing . For mRNA samples: mRNA sequencing was performed using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry. Sample reads were trimmed to remove adapters and low-quality bases using Trimmomatic software and aligned with the reference mouse mm9 genome and Ensemble v70 transcripts using Tophat software. RNA mapping statistics were calculated using Picard software. Library complexity was measured by unique fragments in the mapped reads using Picard's MarkDuplicate utility.
attributes.tag: GEO Accession
attributes.value: GSM3755959