title: 16S rRNA mouse ceccum
design: Barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq.  DNA sequence data was generated using Illumina paired-end sequencing for the V4 region (515F-806R) with Golay barcodes. Amplicons were sequenced on a 151bp x 12bp x 151bp MiSeq run using customized sequencing primers and procedures. A part from the LCM extraction controls, an empty blank was employed during each extraction to account for the possible contamination present in the extraction kits and downstream processing.