title: Entamoeba histolytica sequencing  from HeatShock experiment T0h
design: First passing the cells from 37C to 42C  (T=0h) and then sampling the culture at 2 (T=2h) and 4 (T=4h) hours. An  additional 37C recovering period of 4 h (T=8h) was subsequently  sampled.Total RNA was extracted from approximately 1X106  E. histolytica  trophozoites using Trizol (Invitrogen). The polyA fraction was purified from  10 to 100 g of total RNA using Dynabeads according to the manufacturers  instructions (Thermofisher ref. 28152103011150). Libraries were constructed  using ScriptSeq mRNA-Seq Library Preparation Kit (Illumina) following  manufacturer's recommendations and were quality controlled using Agilent  Bioanalyzer. Sequencing was performed on a HiSeq 2000 (Illumina) to obtain 58  base single-end reads.