title: Culex RVFV
design: small RNA fraction prepared from total RNA fraction of whole mosquito bodies.Mosquitoes  were infected with RVFV by blood feeding and incubated at 28°C for 14 days. The RNA samples were screened for RVFV presence by qRT-PCR using primers RVF FP and RVF RP and RVF probe  A total of 11 bodies corresponding to positive legs/wings with Ct values below 30 were pooled in TRIzol for RNA extraction.