title: GSM2840977: Single-cell_RNA-seq_Subject15_TEMRA-Cell080; Homo sapiens; RNA-Seq
library_construction_protocol: Single cell RNAseq was performed as described in Picelli et al.Nat Protoc. 2014, PMID:24385147. We performed 23 cycles of amplification. Single cell RNAseq was perfromed as described in Picelli et al.Nat Protoc. 2014, PMID:24385147. Barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina) were prepared as following manufacturer's protocol. DNA sequencing - Libraries were sequenced on the Illumina HiSeq 2500  platform to obtain 50-bp single end reads (barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina)).
attributes.tag: GEO Accession
attributes.value: GSM2840977