title: GSM4679570: wild type ZT2 rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA from heads was extracted using TRIzol, treated with TURBO™ DNase (Thermo Fisher Scientific), precipitated and then purified with Lithium Chloride (Thermo Fisher Scientific) following manufacturer's instructions. A total of 1ug of RNA was used to isolate mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA library construction was conducted using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's instructions. Libraries quality and size distribution was verified on a Bioanalyzer, libraries were quantified by real-time quantitative PCR, and 4 multiplexed libraries were mixed in equimolar ratios and sequenced on Illumina Next Seq 500, 75 nt, single end sequencing run
attributes.tag: GEO Accession
attributes.value: GSM4679570