title: GSM2680294: FCNT1_methyl; Taeniopygia guttata; OTHER
library_construction_protocol: Brains were flash frozen in liquid nitrogen, embedded in optimal cutting temperature compound, and 50um sections were cut with a cryostat until 1mm biopsy punch was used to make vertical punches to obtain the hypothalamus and nucleus taenia of the amygdala. Tissue was homogenized with a Mini-BeadBeater-16 (BioSpec Products) using 0.2g of 1mm beads for 10s in TRIzol/TRI Reagent to extract total RNA with a Direct-zol RNA MicroPrep kit (Zymo Research) with a DNase treatment step to remove DNA contamination or in Genomic Lysis Buffer to extract genomic DNA with a Quick-gDNA MicroPrep kit (Zymo Research). Stranded mRNA-Seq libraries were made using KAPA Stranded mRNA-Seq Kit (Kapa Biosystems). Methyl-Seq libraries were prepared according to Urich et al. (2015) Nature protocols 10:475-483.
attributes.tag: GEO Accession
attributes.value: GSM2680294