title: RNA-seq of Gloriosa superba: 5 week-old leaf
design: Gloriosa superba seeds were germinated in potting mix and grown on a growth shelf at ambient room temperature. At 5 weeks post-germination, samples were acquired from leaf, stem, root, and rhizome tissues and immediately snap frozen in liquid nitrogen. Total RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma), and RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).