title: WGS of Sockeye Salmon: Pitt Lake Gynogen Fin
design: Genomic DNA was size selected (40 kbp-80 kbp) and shotgun libraries were prepared using the following kits (10X Genomics Inc.): ChromiumT Genome Library & Gel Bead Kit v2, ChromiumT Genome Chip Kit and ChromiumT i7 Multiplex Kit, and following the Chromium Genome Reagent kits v2 User Guide RevB protocol.  Two libraries (made in the same way from the same animal) were then normalized and pooled.  They were then denatured in 0.05N NaOH and neutralized with HT1 buffer.  ExAMP was then added to the library and it was loaded onto an Illumina cBot at 200 pM HiSeq X for 2x151 cycles (1% phiX library).  Illumina control software HCS HD 3.4.0.38, RTA v. 2.7.7, bcl2fastq2 v2.20