title: RNASeq of H. werneckii on YNB 1.7 M NaCl rep2
design: H. werneckii cells were grown at 28 _C in synthetic defined yeast nitrogen base (YNB) liquid medium (Sigma Aldrich), supplemented with 1.7 M NaCl (equivalent to 10 % (w/v) NaCl) and adjusted to pH 7.0, to mid-exponential phase. 50 ml of culture was pelleted, flash frozen in liquid nitrogen and stored at -80 ĄC until RNA isolation. Pelleted cells were ground in a mortar and pestle maintained in a liquid nitrogen bath and the grindate used for total RNA isolation using TRIzol Reagent according to the manufacturerŐs instructions (Thermo Fisher Scientific). Two independent RNA samples were converted to sequencing libraries using IlluminaŐs TruSeq RNA v2 library preparation kit, and sequenced on a MiSeq (Illumina) to generate paired reads.