title: 16S rRNA gene amplicon sequences of nest  material before incubation
design: Extracted DNAs were shipped to CGEB-IMR (Canada) for polymerase chain reaction (PCR) and sequencing. Polymerase change reaction targeting bacterial V4-V5 region of 16S rRNA gene was performed using modified 515f (5-GTGYCAGCMGCCGCGGTAA-3) and 806r (5- GGACTACNVGGGTWTCTAAT-3) primers. Amplified DNAs were paired-end sequenced (2 x 300 bp) using the Illumina MiSeq platform.