title: Small RNA sequencing from control MCF10A cells
design: Total RNA was purified using Trizol reagent (Invitrogen) according to the manufacturer’s instructions. Small RNA libraries were created using the TruSeq Small RNA preparation kit (Illumina, RS-200-0012) with variable index primers to allow for multiplexing of samples. The protocol provided by the manufacturer was used to create libraries using 0.5 µg total RNA.