title: GSM2759383: 27702 RNA-seq KO LN B cell; Mus musculus; RNA-Seq
library_construction_protocol: About 1-5 ng of cDNA, (Bio-)ChIP-precipitated DNA or DHS-enriched DNA were used as starting material for generating sequencing libraries with the NEBNext Ultra Ligation Module and NEBNext End Repair/dA-tailing module, as described17. For strand-specific RNA-sequencing, the uridines present in one cDNA strand were digested with uracil-N-glycosylase (New England BioLabs) followed by PCR amplification with the KAPA Real Time Amplification kit (KAPA Biosystems). Completed libraries were quantified with the Agilent Bioanalyzer dsDNA 1000 assay kit and Agilent QPCR NGS Library Quantification kit. Cluster generation and sequencing was carried out by using the Illumina HiSeq 2000 system with a read length of 50 nucleotides according to the manufacturer's guidelines
attributes.tag: GEO Accession
attributes.value: GSM2759383