title: 67S rDNA amplicon of soil DNA: soil incubation for Cd remediation
design: Soil incubation experiment was setup to compare the effects of different treatments on Cd behavior under different redox conditions in a paddy soil. There were four treatments: C, control; O, addition of 20 g kg-1 rice straw; S, addition of 300 mg kg-1 sulfur (as gypsum); and F, addition of 20 g kg-1 iron oxide (as hematite). Soil DNA was extracted using the DNeasy PowerSoil Kit (QIAGEN, Germany) following the manufacturers instructions and sent for sequencing the amplicons of the V3V4 regions of the 16 S rRNA gene (amplified using primers 338F and 806R) on an Illumina MiSeq platform.