title: GSM3560385: S-NI-R3; Mycobacteroides chelonae; RNA-Seq
library_construction_protocol: Bacterial RNA were isolated with TRIzol reagent and extracted with chloroform isoamyl. RNA samples were treated with DNases (AMBION) to remove DNA contaminants, purified with the RNA MEGAclear kit (Thermofisher), and depleted of ribosomal RNA with the riboZero kit (Illumina). RNA (total, depleted, purified) is checked on the Bioanalyser system (Agilent) for its quality and integrity. cDNA libraries were prepared with samples displaying a RIN above 7. Libraries were prepared with the TruSeq Stranded RNA LT prep kit cDNA synthesis, set A (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3560385