title: 16S sequencing of mus musculus gut microbiome: Sample PM10.30I
design: "Male C57BL/6J mice were exposed to low-dose penicillin at birth through 30 weeks of age (n = 10), control mice did not receive antibiotics (n = 9 males), and all mice were fed normal chow (Purina Mills International Diet #5001, 13.5% kcal from fat).  DNA was extracted from fecal samples at 4, 16, and 26 weeks of age and cecal and ileal samples at 30 weeks of age by the MoBio PowerSoil kit.  Microbial communities were profiled by sequencing the V1-2 region of the 16S rRNA gene on the Roche 454-platform."