title: 16S sequencing of mus musculus gut microbiome: Sample PML24.30C
design: "Male C57BL/6J mice were exposed to low-dose penicillin at birth through 30 weeks of age. All mice were weaned onto normal chow (Purina Mills International Diet #5001, 13.5% kcal from fat) at 4 weeks of age and half the mice were switched to a high fat diet (Rodent Diet D12451, Research Diets, 45% kcal%) at 17 weeks of age (n = 8 for control NC; 9 for control HFD; 10 for LDP NC; and 10  for LDP HFD).  DNA was extracted from fecal samples at 4, 8, 16, 18, and 30 weeks of age and cecal and ileal samples at 30 weeks of age by the MoBio PowerSoil kit.  Microbial communities were profiled by sequencing the V4 region of the 16S rRNA gene on the MiSeq platorm."