title: Illumina HiSeq 2000 sequencing; qiita_ptid_83:804.3881.1228.4
design: We are now requesting sequencing of additional samples collected from carbonate chimneys at the Lost City hydrothermal field, located near the Mid-Atlantic Ridge at a depth of ~800 m. We have previously shown that extreme (anoxic, pH 9-11, high H2) Lost City chimneys host bacterial communities with strong taxonomic and functional similarities to those of the Newfoundland and Liguria springs mentioned above (Brazelton et al., 2012), but an exhaustive census of these Lost City chimneys has yet to be performed.
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: ACGGTTTCTGGA
attributes.tag: center_name
attributes.value: CCME
attributes.tag: center_project_name
attributes.value: Brazelton_LostCity_chimney_biofilm
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: HiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 3/1/14
attributes.tag: run_prefix
attributes.value: Brazelton_LostCity_chimney_boifilm_lane4_NoIndex_L004
attributes.tag: samp_size
attributes.value: 0.1, g
attributes.tag: sample_center
attributes.value: East Carolina University
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4