title: GSM1419031: meDIP-seq_data_UN2; Mus musculus; MeDIP-Seq
library_construction_protocol: Sperm DNA was extracted by standard phenol-chloroform extraction, with an overnight proteinase K reaction at 55 degrees. DNA was sonicated, paired end adaptors ligated and 12 cycles of whole-genome amplification employed using PhusionTM High-Fidelity Taq and paired end Illumina primers.  Libraries were electrophoresed in 2% 1x TAE agarose gels at 95V in 1x TAE buffer followed by excision of a 200bp range band either 200-400bp to 300-500bp according to the peak fragment size.  Identical size ranges were obtained for control and UN libraries.  qPCR for known methylated and unmethylated regions confirmed library quality.
attributes.tag: GEO Accession
attributes.value: GSM1419031