title: RNA-seq of skin-CT
design: After the total RNA was extracted with Trizol reagent, the total RNA with Polya mRNA was enriched with oligo DT magnetic beads. The magnetic beads were enriched to obtain Polya mRNA. After that, the complete mRNA was segmented by adding fragment reagent, and the length of fragment was about 100nt. The conventional transcriptome sequencing library was constructed directly.