title: GSM3755429: Kidney, Knockout_replicate_4 (ALc1-7); Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from kidneys or cells with Trizol reagent (Life technologies) and further purified with the Directzol RNA MiniPrep kit from Zymo (R2050). 200ng of total RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Ilumina (E7420L) was used. 15 cycles of PCR were done. Quality control of libraries was done via Bioanalyzer High Sensitivity DNA and Tape Station High Sensitivity D5000 Screen Tape. Quantification of the samples was done via qPCR using the BioRad CFX96. The reagent kit for qPCR was the Kapa Library Quantification Kit for Illumina (KK4824).  
attributes.tag: GEO Accession
attributes.value: GSM3755429