title: GSM3172770: Six3KO Retina Rep2; Mus musculus; RNA-Seq
library_construction_protocol: Six3F/F; Six6+/– dams crossed with Six3F/F; CAGGCre-ERTM; Six6+/– male mice were intraperitoneally injected with Tamoxifen (0.15 mL of 20 mg/mL stock solution in corn oil per mice) at E10.5 (approximately 2pm) and E11.5 (approximately 9 am). Retinas from E13.5 embryos (noon) were dissected out in ice-cold PBS, rinsed, and stored overnight at 4 °C. After RNAlater was removed, the samples were stored at -80 °C until purification.   The retinas with the following genotypes were used for RNA isolation using the RNeasy Micro Kit (Qiagen) after genotyping: 1) WT (Six3F/F; Six6+/+), 2) Six3 KO (Six3F/F; CAGGCre-ERTM; Six6+/+), 3) Six6 KO (Six3F/F; Six6–/–), 4) DKO (Six3F/F; CAGGCre-ERTM; Six6–/–). The retinas were homogenized with an electric pellet pestle motor in 500uL of Qiazol and treated with RNase-free DNase set (Qiagen). Total RNA was eluted in 14uL of RNase-free water. The quantity and quality of RNA were verified using Qubit fluorometer (Thermo Fisher Scientific) and Bioanalyzer (Agilent Genomics), respectively. RNA libraries were prepared for sequencing using KAPA RNA HyperPrep Kit with RiboErase
attributes.tag: GEO Accession
attributes.value: GSM3172770