title: RNA-Seq of Pseudomonas aeruginosa: PA2596 and Staphylococcus aureus: SA2599 co-cultured (replicate1)
design: Bacteria were treated with lysostaphin and lysozyme prior to RNAs extraction using RNeasy Plus Mini Kit (Qiagen). RNAs were treated with TURBO DNA-free (Invitrogen). rRNAs were depleted using the Ribo-Zero rRNA Removal Kit (Illumina). The cDNA libraries were carried out using the TruSeq Stranded Total RNA Library Preparation Kit (Illumina)