title: GSM3168423: GMR_PRP8-RNAi_GD_13-19-SampleB_S15; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Fly heads were then collected on dry ice and homogenized with Trizol (Life Technologies) on ice using standard protocols. Following the phase separation, the aqueous phase was transferred into a new tube, mixed with an equal volume of 70% ethanol and loaded directly onto the RNeasy mini kit columns (Qiagen). The rest of RNA isolation was done according to the manufacturer's protocol. On-column DNase digestion (Qiagen) for 15 minutes at RT was always included. Samples were prepared with Lexogen's SENSE mRNA-Seq library Prep Kit. Illumina Next-Generation Sequencing (NextSeq 500) with 300pb paired-end high output run (at ~50M reads per sample) was performed by the Genomics Facility at the Wistar Institute.
attributes.tag: GEO Accession
attributes.value: GSM3168423