title: GSM4568430: KYSE140_H3K27me3_ChIPseq; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde and then neutralized by 1.25M glycine. Cells were sonicated in a Bioruptor Sonicator (Diagenode, USA), followed by cell lysis. Sonicated lysates were incubated overnight at 4 °C with indicated antibody and then were incubated with magnetic beads for an additional 4 h. DNAs were eluted from precipitated immune-complex and subjected to standard Illumina library construction. Libraries were prepared  for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4568430