title: ncRNA-seq of mus musculus: adult female liver
design: A ribosome-depletion workflow was applied to generate the strand-specific cDNA library as described (Parkhomchuk et al., 2009). Specifically, ribosomal RNAs were removed by Epicenter Ribo-Zero Kit (Epicenter). The remaining RNAs were fragmented (250-300 bp), and reversely transcribed using random hexamers and M-MuLV Reverse Transcriptase (RNase H-). After RNA digestion by RNase H, second strand cDNA synthesis was subsequently performed using dNTP (dTTP replaced by dUTP) and DNA polymerase I, followed by purification, terminal repair, polyadenylation, adapter ligation, size selection, and degradation of second-strand U-contained cDNA. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads (PE193) were generated.
identifiers.namespace: SUB6806961
identifiers.id: ML201_212