title: its-cp0
design: "Next generation sequencing library preparations and Illumina Mi Seq sequencing were conducted at ALLWEGENE Inc. (Beijing, China). The bacterial 16S r RNA gene was amplified with primers 338F (ACTCCTACGGGAGGCAGCAG) and 806R(GGACTACHVGGGTWTCTAAT) targeting the V3-V4 region. The fungal 18S r RNA gene was amplified with primers ITS1-F (CTTGGTCATTTAGAGGAAGTAA) and ITS2(TGCGTTCTTCATCGATGC) targeting the ITS1-ITS2 region. The PCR amplification was conducted using specific primers with barcode and high-fidelity Trash Start Fastpfu DNA Polymerase (Trans Gen Biotech,China).The bacterial 16S r RNA gene PCR thermal cycle profile was as follows: 5 min at 95?; 25 cycles of 30 s at 95?, 30 s at 56?, and 40 s at 72?; final 10 min at 72?; and cooling at 4?. The fungal 18S r RNA gene PCR thermal cycle profile was similar to that of the bacterial profile except that it had five more cycles."
identifiers.namespace: Gansu agricultral university
identifiers.id: its-cp0