title: 16S amplicon sequencing salivary gland(also be called the alimentary canal))
design: Champion collards (Brassica oleracea Acephala Group) were grown in a flat for 3 weeks. Four to six squares of wax paper with approximately 100 eggs each of recently oviposited cabbage looper (Trichoplusia ni) eggs (Benzon Research, Carlisle, PA) were pinned to the leaves throughout the flat that had been placed inside a mesh cage.   The second flat of collards was added to the cage when larvae consumed  of the first flat.  Larvae were monitored until they reached 5th instars and were collected for dissection. Larvae were sedated by placing at -200C for 1-2 minutes to facilitate immobilization before dissection of salivary glands, mandibular glands, Malpighian tubules and the alimentary canal.  Five to ten organs (or sets of organs) were pooled for each bioreplicate and frozen.  Midgut tissue was rinsed in PBS (phosphate buffered saline) before freezing.
identifiers.namespace: SUB6303641
identifiers.id: midgut_3