title: RNA-seq of Phakopsora pachyrhizi
design: Frozen leaf tissue was ground in liquid nitrogen and total RNA was extracted using the RNeasy plant mini kit (Qiagen). Total RNA was treated with DNase I (TURBO DNA-free Kit, ThermoFisher Scientific) to remove genomic DNA contamination. Samples were quantified with a Qubit 3.0 fluorometer (Life Technologies) and quality was assessed with a NanoDrop 2000 (ThermoFisher Scientific). The DNA-free total RNA was ribosomal depleted (Ribo-Zero rRNA Removal Kit -Plant Leaf, Illumina). Strand-specific cDNA libraries were prepared using the NEBNext Ultra II RNA library prep kit (catalog # E7765) according to the manufacturers instructions. The cDNA libraries were dual indexed using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1; catalog # E7600S). The libraries were sequenced using HiSeq3000 and MiSeq following the manufacturers protocols.
identifiers.namespace: SUB6298494
identifiers.id: 52_S52_L002