title: RNA-seq of HEK293:rep2
design: Cells were grown in log phase from three biological replicates were collected (derived from successive propagations). Cell pellets were resuspended in RNAlater Stabilization Solution (Invitrogen) according to the manufacturers.  recommendations until RNA extraction. Total RNA was extracted from three replicates of each cell line using Qiagens RNeasy plus Mini Kit according to the manufacturers instructions. Concentrations were determined with a NanoDrop ND-1000 spectrophotometer and RNA quality was assessed on a 2100 Bioanalyzer (Agilent Technologies) using RNA 6000 Nano chips (Agilent Technologies). All samples had an RNA integrity number of at least 9.9. RNA sequencing was performed at GATC using Illumina HiSeq with random primed cDNA library preparation.
identifiers.namespace: SUB6289114
identifiers.id: 134078