title: RNA-seq of rice
design: The library was then subjected to paired-end sequencing on an Illumina HiSeq4000 PE101 platform. The raw image data generated by sequencing were stored in fastq format (Liu et al., 2015). Transcriptome data were analyzed according to Perteas protocol (Pertea et al., 2016). Briey, clean data (high-quality reads) were mapped to the reference genome using HISAT2 v2.0 (Kim et al., 2015). The RPKM values (reads per kilobase per million reads) were calculated using StringTie v1.3.3 (Pertea et al., 2015), followed by dierential expression analysis using the Ballgown package in Bioconductor.
identifiers.namespace: SUB6295920
identifiers.id: NMD24