title: 16s rRNA gene amplicon sequencing  of water column: rajang river
design: ACE Pipeline: The 3.0 m and 0.2  m filters were sent to the Australian Centre for Ecogenomics, Brisbane in  order to undergo amplicon sequencing (16s ribosomal Ribonucleic Acid , rRNA)  using the Illumina Miseq (Caporaso et al. 2012) platform. It should be noted  that the platform utilized runs on the forward (F1) read only. The raw .fastq  files were first processed with fastqc (Babraham Bioinformatics). Using the  Trimmomatic software (Bolger, Lohse and Usadel 2014), the primer sequence was  removed by trimming the first 20 bases of all fastq files. Then, the  primer-trimmed fastq files were then quality trimmed with the criteria of an  average base quality of >15 with a sliding window of 4 bases.
identifiers.namespace: SUB6301697
identifiers.id: AUG16RAJST24PA