title: TruSeq synthetic long-reads from D. melanogaster used for de novo assembly
design: High molecular weight DNA was isolated from pooled samples of the y;cn,bw,sp strain of Drosophila melanogaster, 50-100 pooled adult individuals of both sexes. To produce each library, extracted DNA was sheared into approximately 10 Kbp fragments, ligated to amplification adapters, and diluted to ~200 molecules per well on a 384 well-plate.  DNA pools were then amplified by long range PCR, and barcoded libraries were prepared within wells using Nextera-based fragmentation and PCR-mediated barcode and sequencing adapter addition. Libraries were sequenced on the Illumina HiSeq platform, then assembled into long-reads using a proprietary assembler that accounts for molecular biology steps in the library preparation protocol.
identifiers.namespace: Stanford University
identifiers.id: TruSeq synthetic long-reads