title: RNA from Panagrolaimus davidi thawed from -1 to +1 degrees
design: For the Illumina sequencing the nematodes were cultured in S medium at 20°C for 3 weeks and fed every 3-4 days with Escherichia coli. After cold acclimation (held at +5°C for three days), nematodes were cooled from +1°C to -1°C at 0.5°C min-1, and the sample frozen by adding a small ice crystal and maintained at -1°C for 24 h.  After which the nematodes were warmed to +1°C at 0.5°C min-1 and allowed to recover for 24 h at 20°C.  Nematodes were extracted using a modified Baermann technique. The worms were snap frozen in 1.5ml microcentrifuge tubes at -80 degrees Celsius and preserved at -80 degrees Celsius until extraction. RNA was extracted from whole worms using TRI-sure (Bioline) according to manufacturer's instructions and purified on Qiagen RNeasy columns. RNA were checked for purity on standard agarose gels and quantified using a NanoDrop spectrophotometer. 5ug from 8 different treatment stages was sent for sequencing.
identifiers.namespace: British Antarctic Survey
identifiers.id: Panagrolaimus davidi +1 degrees