title: Deep sequencing by 454 of mouse B cell immunoglobulin sequencing
design: Differing populations of activated B cells were cell sorted before RNA extraction.Immunoglobulin sequences were amplified using Phusion High-fidelity DNA Polymerase. Products were then deep-sequenced by 454 pyrosequencing on the Genome Sequencer FLX system (Roche).
identifiers.namespace: SUB3155805
identifiers.id: TCRgdko_GC_IgG1_IgE_DMBA