title: HepG2-circ2176
design: The RNA was purified from the cells using Trizol (Invitrogen, USA) following the manufacturers instruction. The RNA was quantified by Qubit RNA assay (Thermo Fisher, USA) and the quality was evaluated by Agilent bioanalyzer 2100 (Agilent, USA). About 1000ng of the RNA was used for the library preparation using VAHTST mRNA-seq v2 Library Prep Kit (Illumina, USA). In brief, RNA with polyA tail was captured by the Dynaloligo (dT) beads. The RNA was fragmented and then reverse-transcribed into double-strand cDNA by random hexamer primers. The end of the cDNA was repaired by the End-It DNA EndRepair kit and then treated by A-addition. The modified cDNA was ligated to adapters and subjected to PCR amplification. The library quality was determined by Agilent bioanalyzer 2100.
identifiers.namespace: SUB5231892
identifiers.id: HepG2-circ2176