title: DNA-Seq of viral symptomatic adult bean samples
design: DNA extraction from 12 viral symptomatic leaf samples was carried out using the Quick-DNA plant/seed miniprep kit (Zymo Cat log D6020). Rolling cycle amplification was then carried out on each of the DNA samples using the TempliPhi 100 amplification kit as described by the manufacturer (GE). Library preparation was  carried out on the individual samples using the Nextera DNA library preparation kit Illumina as described by the manufacturer (Illumina, San Diego, CA, USA). Samples were dual-indexed with unique barcodes before a PCR enrichment step. For each library, quantification and determination of the correct insert size was carried out using the Agilent 2200 tape station (Agilent) with the D1000 screen tape and ladder. Libraries with the correct insert size and concentration were sequenced with 2 x 150 bp paired-end reads on a Hiseq2500 at Macrogen, South Korea.
identifiers.namespace: SUB4122781
identifiers.id: SRF035_1