title: P1604STC2SHrna marine sediment 16S 97m depth, sediment trap, RNAlater
design: DNA from water-column and trap samples was extracted using the NucleoMag 96 Plant kit (Macherey Nagel) following the manufacturer's instructions and amplified by Polymerase Chain Reaction using the Q5 high-fidelity PCR kit.  The V4-V5 region of the 16S small subunit ribosomal RNA gene (SSU-rRNA) was targeted using primers 515F and 926R. The PCR products were pooled in equimolar amounts (~10 ng/ul) and sequenced using a dual-barcode index on an Illumina MiSeq platform.
identifiers.namespace: SUB3916515
identifiers.id: P1604STC2SHrna