title: RNA-Seq of Urechis unicinctus: early-segmentation larva
design: Approximately 5 g of total RNA was used to construct a cDNA library following the protocols of the Illumina TruSeq RNA Sample Preparation Kit: purifying the mRNA using oligo(dT) and mRNA fragmented; RT-PCR; repairing process; generating the cDNA library and amplifing; DNA quantitation; forming clonal clusters using cBot; sequencing on an Illumina Hiseq with 2*150 bp paired-end (PE) reads.
identifiers.namespace: SUB4374986
identifiers.id: library6