title: P1604STC1SHrna marine sediment 18S 100m depth, sediment trap, RNAlater
design: DNA from water-column and trap samples was extracted using the NucleoMag 96 Plant kit (Macherey Nagel) following the manufacturer's instructions and amplified by Polymerase Chain Reaction using the Q5 high-fidelity PCR kit. The V9 region of the 18S rRNA gene was targeted using primers 1389F and 1510R. The PCR products were pooled in equimolar amounts (~10 ng/ul) and sequenced using a dual-barcode index on an Illumina MiSeq platform.
identifiers.namespace: SUB3916597
identifiers.id: P1604STC1SHrna_18S