title: Kinetic transctiptome of Escherichia coli following induction of sigma-E (RpoE)
design: 20 g of total RNA was ligated with the RNA adaptor PSS-tag (5'- GCAUAGGGGUAAA-3') using the T4 RNA ligase I (New England Biolabs). Samples were then treated with tobacco alkaline phosphatase (TAP; EPICENTRE Biotechnologies) and ligated to the TSS-tag RNA adaptor (5'-GCGAGACUGAGAA-3').
identifiers.namespace: SUB6278921
identifiers.id: ECkin_10_TAP+_rep2