title: Paired end reads used to assemble Marthasterias glacialis de novo ovary transcriptome
design: Whole ovary was dissected from gravid females and put in Trizol. RNA was extracted and then cleaned with a Qiagen RNeasy Micro column with on-column DNA digestion. The sequencing libraries were prepared with Illumina reagents, (mRNA-Seq Sample Prep Kit for GAIIx samples or TruSeq Sample Prep Kit for HiSeq samples) with the maximum recommended RNA input. The protocol was followed exactly with the addition of a gel selection step, (agarose gel for GAIIx samples or Caliper LabChip XT for HiSeq samples) prior to PCR amplification.
identifiers.namespace: Brown University
identifiers.id: AMR0029-Marthasterias glacialis-RNAseq