title: MLV integration site junctions in human CD34+ cells
design: Human CD34+ cells were infected with MLV vector for 4 days.  Genomic DNA was purified and sheared to 300-500bp.  And then end repaired and A tailed.  A partially double stranded linker with a T overhang was ligated to the fragmented genomic DNA.  MLV integration junctions were amplified by PCR using one primer specific for the MLV-LTR and one primer specific for the linker.  Illumina sequencing primers and adaptors were attached with nested PCR.  The PCR products were sequenced on Illumina HiSeq with 2x105bp PE kit.
identifiers.namespace: Frederick National Laboratory for Cancer Research
identifiers.id: MLV_CD34_integrationJunctionHiSeq