title: Differential gene expression according to race and host plant in the pea aphid
design: Typically the RNA of 20 heads from wingless adult aphids per clone/test plant combination was extracted and pooled for RNA-seq analysis. RNA extractions were performed using the Macherey Nagel NucleoSpin RNA II kit, following the manufacturer’s instructions. Barcoded RNAseq library was prepared using Illumina TruSeq RNA Sample Prep Kit v2 using 1 μg of total RNA input and 10 PCR cycles, as per the manufacturer's recommendations. Final library were quantified by qPCR and combined into 6-plex pools prior to sequencing
identifiers.namespace: INRA
identifiers.id: Pea Aphid host races RNASeq