title: 16S rDNA-seq of soil sample  where crocodile lizard with nodule were found
design: Total DNA was extracted from the epidemic materials (nodules), water samples and soil samples using a PowerFecal DNA Isolation Kit (MOBIO Laboratories, Inc., USA) with 20 mg/ml lysozyme. For bacteria community analysis, the V4 hypervariable region of 32Smers of 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). Amplicon library was prepared using NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA).
identifiers.namespace: SUB4199385
identifiers.id: SS.LK.06