title: E. coli Type I persistence differential expression
design: Type I persisters were extracted from an stationary phase culture of E. coli TH1269 (hipA7) by individually inoculating 6 flasks with 10 μL of an overnight culture. Each replica was incubated at 37⁰C and 200 rpm for 16 hours. After reaching the stationary phase, the bacterial cultures were pelleted and immediately processed. To extract total RNA from type II persister cells, persisters were first isolated by employing the protocol described in Cañas-Duarte et al (Cañas-Duarte et al., 2014), and the RNA from the lysed non-persister cells was completely degraded using RNAse A (Sigma-Aldrich, St. Louis, USA) before proceeding with the total RNA extraction. The complete degradation of the RNA from the non-persister cells was determined with a gel electrophoresis of RNA extractions of the supernatant. Prior to the extraction of the total RNA from persisters, the pellets were washed three times.
identifiers.namespace: Laboratorio Nacional de Ciencia e Tecnologia do Bi
identifiers.id: hipA7Transcriptome