title: Water-well Chapula
design: Two hundred and five ml well water was filtered through 0.22-μm filter membranes (Millipore, MA, USA) to concentrate the suspended bacteria. The microbial pellets were used for metagenomic DNA isolation.  Amplicon libraries of V1–V3 regions of 16S rRNA genes were obtained using 10 pb-barcoded primers [8−F (5´−AGA GTT TGA TCI TGG CTC A− 3´) and 556−R (5´ −TGC CAG IAG CIG CGG TAA− 3´)]. Each sample was amplified three times, pooled and purified using the UltraClean™ PCR Clean-up kit (MoBio, Carlsbad, CA, USA). Equal concentrations of the purified library amplicons were sent to Macrogen Inc. (DNA Sequencing Service, Seoul, Korea) for pyrosequencing. Pyrosequencing was done with a Roche GS−FLX Titanium 454 pyrosequencer (Roche, Mannheim, Germany).
identifiers.namespace: Cinvestav
identifiers.id: Water-well Chapula