title: RNA-Seq of B. terrae BS001 growing alone 2: Sample T2C3
design: The sequencing project was done at Macrogen South Korea. The mRNA in total RNA converted into a library of template molecules suitable for subsequent cluster generation using the reagents provided in the Illumina ® TruSeq™ RNA Sample Preparation Kit. The first step in the workflow involves purifying the poly-A containing mRNA molecules using poly-T oligo-attached magnetic beads. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then go through an end repair process, the addition of a single "A" base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
identifiers.namespace: University of Groningen
identifiers.id: T2C3