title: cBKV06
design: Genetic material was centrifuged from 250 ml of water in bait or pond fish retailer tanks and DNA extracted with Qiagen DNeasy kits. Extractions were subjected to a two step library prep protocol. The first step utilized targe primers with spacer inserts of varying lengths (7-14 bp) and a portion of the Illumina MiSeq sequencing primer. The second step used Illumina Nextera indices, added to amplicons via PCR. These libraries were sequenced on an Illumina MiSeq with 2X300 chemistry.
identifiers.namespace: SUB7741208
identifiers.id: cBKV06