title: Control of XK
design: Heat sensitive variety ‘GHA’ and tolerance variety ‘XK’  of non-heading Chinese cabbage were used in this study.The transcriptome of these varieties for contral and under 1h, 6h, 12,18h of heat stress were analyzed using RNA-seq.Total RNA were extracted from the leaves of these conditions. mRNAs were purified from the total RNA using poly-T oligo-attached magnetic beads. The derived mRNAs were then fragmented and the first strand cDNA was synthesized using random hexamer. Second-strand cDNA was synthesized using DNA polymerase I and RNase H. The double-stranded cDNAs were purified and ligated to adaptors for Illumina paired-end sequencing. The quality and quantity of the library was verified using an Agilent 2100 Bioanalyzer and ABI real time RT-PCR. The cDNA libray was sequenced using the Illumina HiSeq2000 platform. 
identifiers.namespace: Wuhan Institute of Vegetable Science
identifiers.id: Control of 'XK'