title: small RNA sequences of sterile line
design: In brief, 20 µg of total RNA were used for sequencing library preparation. The small RNA fractions with the length of 15-40 nt were isolated by polyacrylamide gel electrophoresis and with proprietary adaptors (Illumina, USA). The short RNAs were then converted to cDNA by RT-PCR. The PCR products were size-fractionated and recovered for sequencing using Illumina's Genome Analyzer II according to manufacturer’s instructions (Illumina, USA).
identifiers.namespace: Institute of Cotton Research, Chinese Academy of A
identifiers.id: ZBA results