title: Bacterial 16S rRNA fragments from Gulf of Gdansk sampled in July 2011, replicate A
design: First strand of metatranscriptomic cDNA was used as templated in the first round of PCR with primers bac341f (CCTACGGGNGGCWGCAG) and bac805r (GACTACHVGGGTATCTAATCC) (Herlemann et al. 2011). The primers carried M13 and M13R overhangs. In the second round of PCR amplicons from the first round served as template and the primers were M13 and M13R with A and B adaptors and MID sequences (barcodes) in overhangs. Two barcodes were used to identify each sample.
identifiers.namespace: Nicolaus Copernicus University
identifiers.id: Hel_jul_A