title: RNA-Seq of rrp1-3 CRISPR Arabidopsis
design: Six Arabidopsis plants were randomly taken out from the frozen samples for every RNA isolation and cDNA synthesis. RNA was extracted using the RNeasy plant mini kit (Qiagen, Dusseldorf, Germany). DNase digestion was performed to remove contaminating DNA using the RNase-Free DNase set (Qiagen), then the RNA samples were processed using the RNA library prep kit (New England BioLbs, Ipswich, MA, USA) and sequenced by the Illumina HiSeq2000 platform.
identifiers.namespace: SUB7459400
identifiers.id: CRISPR1_combine