title: RNA-seq of Lactobacillus acidophilus NCFM stationary phase in PBS Day0 prior gavage biological replicate 1
design: Cells were grown in MRS broth at 37C for 16 h, washed once with PBS pH 7.4 and resuspended in PBS. Portion of the cell suspension was pelleted and flash frozen for RNA isolation as in vitro control samples (whereas the other portion was administered to 6 germ-free mice for in vivo gene expression studies).  RNA was extracted with the Zymo Direct-zol RNA miniprep kit, treated with DNase I and column-purified. RNA purity was confirmed via Bioanalyzer and sent to UIUC for sequencing.
identifiers.namespace: SUB3892263
identifiers.id: LaNCFM_Day0_PBS-A