title: RNA-Seq of Haliotis tuberculata: Saint Malo control adult rep2
design: Sampling took place during an experiment of successively infected adult abalone from two different natural populations in Britany (Molène and Saint-Malo) with the bacteria Vibrio harveyi. Hemolymph was sampled 72h post-infection, following the third exposure for abalones from Saint-Malo and the first exposure for those from Molène. Non-infected abalones were sampled at the same time for both populations. Three replicates were sampled per treatment and stored in TRIzol reagent (Life Technologies) at -80°C until the RNA extraction. They were homogenized with steel beads before extraction of total RNA according to manufacturer's instructions. The quantity and quality of extraction was determined using an Agilent 2100 RNA BioAnalyser (Agilent, Santa Clara, CA, USA). Library construction and Sequencing was performed by Gene pool, University of Edinburgh, Scotland, on a llumina HiSeq 2000 (100 bp paired-end). Two runs were performed with lanes of 12 samples multiplexed. 
identifiers.namespace: University of Brest
identifiers.id: TP-018_ST340