title: in situ DNase Hi-C from embryos
design: Strains were maintained in population cages on molasses agar with yeast paste and 8-16 hour embryos were collected and dechorionated in 50% commercial bleach for 2.5 minutes. Nuclei were isolated from ~200mg of embryos and fixed in 1.8% formaldehyde for 15 minutes according to the protocol in (Sandmann, et al. 2006). In situ Hi-C chromosome conformation capture was performed according to the previously published DNase-based protocol (Ramani, et al. 2016)
identifiers.namespace: SUB5218393
identifiers.id: DGRP379_HiC