title: 16S rRNA gene Diversity of Lake Tyrrell
design: Amplicons were generated targeting the V3-V4 hypervariable region.Second-round PCR amplification was conducted on the initial template, with indexed adaptors being added to generate indexed libraries. DNA libraries were validated by Agilent 2100 Bioanalyzer, and quantified. DNA libraries were multiplexed and sequenced using Illumina MiSeq. After removing primers and adapters, raw data was quality controlled using FastQC and MultiQC. Based on the generated quality reports, Trimmomatic was used to trim and crop the data and remove any remaining Illumina adapter, and paired reads were joint using BBMerge.
identifiers.namespace: SUB6299062
identifiers.id: C3_R1