title: RNA from Panagrolaimus davidi -10 degrees unfrozen
design: For the Illumina sequencing the nematodes were cultured in S medium at 20°C for 3 weeks and fed every 3-4 days with Escherichia coli. The nematodes were acclimated at +5°C for 3 days, after which they were cooled to -10°C at 0.5°C min-1. When this temperature was reached, it was noted whether the medium had frozen or not, and those in unfrozen medium removed for extraction. Nematodes were extracted using a modified Baermann technique. The worms were snap frozen in 1.5ml microcentrifuge tubes at -80 degrees Celsius and preserved at -80 degrees Celsius until extraction. RNA was extracted from whole worms using TRI-sure (Bioline) according to manufacturer's instructions and purified on Qiagen RNeasy columns. RNA were checked for purity on standard agarose gels and quantified using a NanoDrop spectrophotometer. 5ug from 8 different treatment stages was sent for sequencing.
identifiers.namespace: British Antarctic Survey
identifiers.id: Panagrolaimus davidi -10 unfrozen