title: GBS of Impatiens capensis genomic DNA
design: Digested using restriction enzyme ApeKI and standard protocols by University of Wisconsin-Madison Biotech Center; samples pooled/multiplexed 96 per flowcell (sequencing) lane and sequenced in two lanes to give effective sequencing of 48 samples per lane; reads demultiplexed, filtered, and trimmed using process-radtags (Stacks program) and cutadapt program (original reads = 101bp - 8bp for longest barcodes - 4bp for left hand restriction enzyme cut site = all final reads 89bp long); removed any reads that contained the common adapter sequence, uncalled bases, or a phred score <10 (sliding window 15% of read length).
identifiers.namespace: SUB5218615
identifiers.id: AC9MEBANXX_icapensis-plateBT3_F5_CATCGT