title: 16S rRNA gene sequencing of methanogenic enrichment culture: oilfield produced water
design: 16S rRNA gene fragments from a 25 mL aliquot of produced water enriched with heavy oil (for 17 months) were amplified using universal PCR primers 926F and 1392R. Nextera XT i7 and i5 adapters were added to purified amplicons using a second round of PCR. The final purified and normalized amplicons were sequenced using a MiSeq Reagent Kit v3. Raw forward and reverse sequence reads were merged in QIIME.
identifiers.namespace: SUB3193217
identifiers.id: Heavy_oil_17month_R1