title: Mesodinium rubrum RNA-Seq from Day 1 cultures grown in high light replicate 1
design: M. rubrum cultures were maintained in F/2-Si media in 32 PSU seawater at 4C and at 5 and 65 umol photons m-2 s-1 light and fed Geminigera cryophila (CCMP 2564) at a ratio of ~5:1 when transferred, once per week. To collect cell material for RNA extractions, M. rubrum cultures were gently filtered on to 1 um polycarbonate filters, and flash frozen in liquid nitrogen for later analysis. Frozen cell pellets were extracted for RNA using a standard Trizol procedure
identifiers.namespace: SUB6178665
identifiers.id: MrHH1T1