title: RNA-seq of human K562 cell line, transfected with miR-22 overexpression vector (PIG/miR-22), 48 hours post transfection
design: Bulk K562 was transfected with PIG/miR-22 using Lipofectamine 2000 per manufacturers protocol. Total RNA was isolated with the Qiagen miRNeasy Mini Kit. NEBNext Ultra RNA Library Prep Kitfor Illumina by following manufacturers recommendations (NEB, Ipswich, MA, USA). Briefly,mRNA were first enriched with Oligod(T) beads. Enriched mRNAs were fragmented for 15minutes at 94 C. First strand and second strand cDNA were subsequently synthesized. cDNAfragments were end repaired and adenylated at 3ends,and universal adapter was ligated tocDNA fragments, followed by index addition and library enrichment with limited cycle PCR.
identifiers.namespace: SUB4124750
identifiers.id: K562:OE48