title: Archaeal community in sewer pipe biofilm during nitrate dosage
design: DNA was extracted from collected biofilm samples using RNA PowerSoilTM Total RNA Isolation Kit and DNA Elution Accessory kit according to manufacturer instructions (MOBIO Laboratories, Inc, Carlsbad, CA). The extracted DNA was quantified using a Qubit® 2.0 Fluorometer (Invitrogen Molecular probes Inc., Oslo, Norway) and were subsequently analyzed through tag-encoded FLX-Titanium amplicon pyrosequencing at the Research and Testing Laboratory (Lubbock, TX, USA). Briefly, genomic DNA from the biofilm communities was used as a template in PCR reactions using primers 341F/958R (Archaea) targeting the V1-3 region of the 16S rRNA gene complemented with 454-adapters and sample-specific barcodes. Raw sequence dataset was pre-processed at RTL facilities to reduce noise and sequencing artifacts as previously described. The aim of this study is to identify changes in archaeal communities caused by nitrate addition.
identifiers.namespace: Catalan Institute for Water Research (ICRA)
identifiers.id: Sewer pipe biofilm (Archaea) during nitrate dosage