title: RNA-Seq of Lactobacillus acidophilus: stationary phase
design: Total mRNA was collected from cultures at stationary. Cultures were flash frozen and RNA was extracted with the ZymoDirect-zol RNA miniprep kit. cDNA fragments were generated. Reads are 150nt in length.  Ribosomal RNAs were removed with the Ribozero Bacteria kit (Illumina) to prepare the RNAseq libraries.  The stranded RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNA Sample Prep kit' (Illumina). The libraries were pooled in equimolar concentration and each pool was quantitated by qPCR and sequenced on one lane for 151 cycles   on a HiSeq4000 using a HiSeq sequencing kit version 1. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina).
identifiers.namespace: SUB3916713
identifiers.id: LacStat