title: RNA-Seq of asio flammeus: retina
design: Constructed using TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturers instructions. Libraries were individually tagged with the NEXTflex RNA-Seq Barcode adapter set (Bio Scientific). The libraries were sequenced in multiplex in one 300-bp paired-end run on an Illumina MiSeq platform (at the DNA Sequencing Facility, Department of Biology, Lund University) using the MiSeq Reagent Kit V3 (Illumina, cat no. MS-102-3003).