title: Bottle inoculated with Groesbeek activated sludge from December 2019  spiked with 30nM micropollutants at time 4 days replicates pulled together
design: 16S rRNA gene sequencing was performed on an Illumina Miseq.  The primers used for previous PCR amplification were Bac341F  (5CCTACGGGNGGCWGCAG3) and Bac785R (5 GACTACHVGGGTATCTAATCC 3)  (Klindworth et al., 2013).