title: Shifts in microbial community structure and function in light- and dark-grown biofilms driven by warming
design: Three replicate samples from the inoculum biofilm stream community were subjected to DNA extraction using  UltraClean™ Soil DNA Extraction Kit (MoBio Laboratories Inc.) according to manufacturer instructions. DNA from each replica were pooled in equimolar ratios and used as template in PCR reactions using primers 357F/907R targeting the V3/5 region of the bacterial 16S rRNA gene complemented with 454-adapters and sample-specific barcodes. Pyrosequencing was performed in a Roche Genome Sequencer 454 GS FLX Titanium using optimized protocols developed by the sequencing company (Research and Testing Laboratory, Lubbock, TX).