title: GSM4290728: QDG1-smallRNAseq; Rattus norvegicus; miRNA-Seq
library_construction_protocol: The hearts were dissected and cardiac tissues were stored in liquid nitrogen and stored in low temperature.Total RNA was extracted with Trizol (Tiangen, Beijing, China). RNA integrity was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and RNA concentration was measured by Qubit RNA Assay Kit in a Qubit Fluorometer (Invitrogen, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEB Next®Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
attributes.tag: GEO Accession
attributes.value: GSM4290728