title: GSM4141520: circRNA PRV-DX-3; Sus scrofa; ncRNA-Seq
library_construction_protocol: Total RNA was isolated from infected cells using SuPerfecTRITM Total RNA Isolation Reagent (Pufei) according to the manufacturer. A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly, ribosomal RNA was removed by Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Then, linear RNA was removed by RNaseR digeston.  Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. For miRNA, sequencing libraries were generated using Small RNA Sample Pre Kit according to the manufacture.
attributes.tag: GEO Accession
attributes.value: GSM4141520