title: GSM1141672: H3K4me3-NativeChip; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were rinsed with PBS and treated at 37C with MNase for 5 min, before quenching with MNase stop buffer and the chromatin was sheared to 200-500 bp fragments using a Fisher Dismembrator. Chromatin fragments were immunoprecipitated with specific antibodies at 4 °C with gentle rotation. Antibody-chromatin complexes were washed and eluted and treated with RNase-A. Following proteinase K treatment, the DNA fragments were purified by phenol-chloroform-isoamyl alcohol extraction and ethanol precipitation. A quantity of 20-50 ng of ChIP DNA was end-repaired, followed by the addition of adenine, ligation to Illumina adapters, and creation of a Solexa library for sequencing. 20-50 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer’s protocol.
attributes.tag: GEO Accession
attributes.value: GSM1141672