title: GSM3178292: hnRNPA1-eCLIP-repA; Homo sapiens; OTHER
library_construction_protocol: eCLIP was performed as previously described (Van Nostrand et al. 2016) with the exception of using nuclear extract prepared from K562 cells instead of whole cell extract, with the following antibodies: monoclonal anti-hnRNPA1 antibody, clone 9H10 (Sigma-Aldrich Cat# R4528 RRID:AB_261962), monoclonal anti-hnRNPA1 antibody, clone 4B10 (Sigma-Aldrich Cat# R9778 RRID:AB_477477), and polyclonal anti-DDX5 antibody (Abcam Cat# ab21696 RRID:AB_446484). eCLIP was performed as previously described (Van Nostrand et al. 2016)
attributes.tag: GEO Accession
attributes.value: GSM3178292