title: GSM4138751: LDG_SLE_01_mat [ATAC-seq]; Homo sapiens; ATAC-seq
library_construction_protocol: Peripheral blood was collected by heparinized tubes and the PBMC fraction was obtained using Ficoll/Hypaque gradient. LDGs were sorted by flow cytometry using CD10-APC,CD14-FITC and CD15-PE antibodies. ATAC-seq was performed according to a published protocol (Buenrostro et al., 2013) with minor modification. 100,000 cells performed in quadruplicate for each sample were treated with the Tn5 transposase (Illumina) for 30 min at 37°C and PCR-purified (Qiagen) to generate library fragments. ATAC seq data were generated on Illumina HiSeq 2500 instruments according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4138751