title: internal transcribed spacer (ITS) region sequences of soil microbiome: young-aged lawn with agricultural-history, 0-30 cm depth, leaf litter low-rate, Oct-2017
design: Qiagen DNeasy PowerSoil DNA Isolation Kit; PCR amplification with fungal primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and 58A2R (5-CTGCGTTCTTCATCGAT-3)