title: 16S amplicon-Seq of Trichodesmium bloom biomass 2h after incubation, bottle100
design: amplified with conserved bacterial primers 27F and 1100R (Dowd et al., 2008) using a high fidelity polymerase (Phusion DNA polymerase, Thermo Scientific), second PCR with fusion primers containing individual tags per sample; AMPure magnetic purification; fragment size for sequencing ~340 nt 