title: GSM4289813: X1; Mus musculus; RNA-Seq
library_construction_protocol: GAD67-GFP+ offspring brains were fixed in 4% paraformaldehyde and cryoprotected (Optimal Cutting Temperature Compound, Fisher Scientific). Laser capture microdissection (LCM) was utilized to isolate migrating GAD67-GFP+ cells from the cortical plate of fixed embryonic brain 10 µm tissue slices on metal frame PET foil slides (Leica) from one male embryo per litter (n=4 per group). Approximately 100 individual GFP+ cells were collected at the leading edge of migration from each of 10 tissue sections, giving approximately 1,000 cells per sample. RNA was isolated from the tissue using the RecoverAll Total Nucleic Acid Isolation kit (Thermo Fisher Scientific, AM1975) Libraries were prepared using the Trio RNAseq kit (Nugen)
attributes.tag: GEO Accession
attributes.value: GSM4289813