title: RNA-Seq of Mus musculus: ~4 weeks male spleen
design: Total RNA was isolated from spleen. Stranded libraries were prepared using the KAPA mRNA HyperPrep Kit (KAPA Biosystems): polyA containing mRNA was isolated using oligo-dT magnetic beads; RNA fragmented; first and second strand cDNA synthesized; Illumina-specific adapters containing a unique barcode sequence for each library were ligated; and PCR amplification performed. Libraries were checked for quality and concentration using the DNA 1000 assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturers' instructions. Libraries were pooled and sequenced.