title: Illumina HiSeq 2000 paired end sequencing; Posttranscriptional mRNA expression changes in stress
design: Posttranscriptional mRNA expression changes in stress
library_construction_protocol: Cells were seeded 48 hours prior to treatment. DMSO was subsequently added to cells  as a solvent control. Cells were harvested in PBS 3 hours later. Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. 3'T-fill libraries were created as described in Wilkening S, et al. An efficient method for genome-wide polyadenylation site mapping and RNA quantification. Nucleic Acids Research. 2013;41(5):e65. doi:10.1093/nar/gks1249.
attributes.tag: Experimental Factor: Extraction protocol
attributes.value: cyto
attributes.tag: Experimental Factor: Nucleic acid library construction protocol
attributes.value: 3T-fill
attributes.tag: Experimental Factor: compound
attributes.value: DMSO