title: PCR amplicon sequencing of 293T cells: 400-SNPs panel designed by MPPD-Online
design: For the 400-SNPs panel, library construction was accomplished using 2 rounds of PCR. In the first round, target SNPs were amplified by multiplex PCR, and a small fraction of the adapter sequence was introduced. In the second round of PCR, the complete sequencing adapter was added, and the PCR products were ready for sequencing.