title: RNA-seq of Burkholderia dolosa carrying fixL variants
design: Burkholderia dolosa AU0158 carrying an ancestral variant of fixL was grown to log phase in LB media, RNA was extracted using Invitrogen RiboPure RNA Purification Kit, bacteria, rRNA was depleted using the MICROBExpress Bacterial mRNA Enrichment Kit (Ambion), and RNA was fragmented using NEBNext Magnesium RNA Fragmentation Module (NEB).  The library was generated using NEBNext Multiplex Small RNA Library Prep, and samples were sequenced using single end 50 bp reads using the Illumina HiSeq2500. Reads were trimmed using Trimmomatic tool.