title: GSM4292026: CONT_3; Mus musculus; RNA-Seq
library_construction_protocol: RNeasy MiniPlus kit (Qiagen) Purified total RNA was first evaluated for its quantity, and quality, using Agilent Bioanalyzer 2100. All RNA samples had a RIN (RNA Integrity Number) of eight or higher. Each library was quantified, and its quality accessed by Qubit and Agilent Bioanalyzer, and multiple libraries were pooled in equal molarity. Average size of library insert was approximately 110b. Pooled libraries were applied to Ion Sphere Particles (ISP) with template preparation and amplification using Ion OneTouch 2, followed by ISP loading onto a PI chip and sequencing on Ion Proton semiconductor (Life Technologies). A Phred quality score (Q score) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy).
attributes.tag: GEO Accession
attributes.value: GSM4292026