title: GSM3439145: CantonS; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was extracted from 3rd instar larvae using the Trizol reagent (Invitrogen, Carlsbad, CA, USA). The isolated RNAs were used for the construction of single-end mRNA-seq libraries, using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MS, USA) according to the manufacturer's recommendations. mRNA-seq was performed on an Illumina Hi-seq 1000 instrument (Illumina, San Diego, CA, USA) using 51-bp single-end reads. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM3439145