title: WES of human: kidney
design: Individual exome capture of each DNA sample followed by single-indexed library generation was carried out using the SureSelect XT Target Enrichment System (Agilent Inc).  200ng to 3ug of DNA was fragmented to a size range of 150bp-200bp followed by end repair, adaptor ligation, and low cycle (6-10 cycles) PCR.  Libraries were purified and validated for appropriate size (225-275bp) on a 2100 Bioanalyzer DNA1000 chip (Agilent Technologies, Inc.).  750ng of purified library was hybridized to the SureSelectXT All exon V5 Capture library (Agilent Inc.) for 18 hours at 65буC. The captured regions were bound to streptavidin magnetic beads and washed to remove any non-specific bound products.? The eluted library underwent a second 12 cycle PCR amplification to add sample specific barcodes necessary for multiplexing.  Final libraries were purified, measured using a Bionanalyzer (250-350bp), and quantitated using KAPA qPCR.