title: 16s seq of fecalNCHD
design: The 16s ribosomal RNA were sequencing on the Illumina platform. The 16S rRNA genes of distinct regions (V3-V4) were amplified used specific primers (341F: CCTAYGGGRBGCASCAG; 806R: GGACTACNNGGGTATCTAAT) Sequencing libraries were generated using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA). The library was sequenced on an Illumina HiSeq2500 platform, and 250-bp paired-end reads were generated.