title: GSM3439238: anti_rep3; Populus tomentosa; RNA-Seq
library_construction_protocol: Total RNA was isolated from suspension cells of populus tomentosa, using EASYspin Plant RNA kit RN09 (Aidlab, China) according to the manufacturer's instructions. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 μl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
attributes.tag: GEO Accession
attributes.value: GSM3439238