title: Illumina HiSeq 2000 sequencing; Posttranscriptional mRNA expression changes in stress
design: Posttranscriptional mRNA expression changes in stress
library_construction_protocol: Cells were seeded 48 hours prior to stress treatment. Cells were subsequently treated with 5 ??g/ml Anisomycin for 3 hours and harvested in PBS. Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. 3'T-fill libraries were created as described in    Wilkening S, et al. An efficient method for genome-wide polyadenylation site mapping and RNA quantification. Nucleic Acids Research. 2013;41(5):e65. doi:10.1093/nar/gks1249.
attributes.tag: Experimental Factor: Extraction protocol
attributes.value: cyto
attributes.tag: Experimental Factor: Nucleic acid library construction protocol
attributes.value: standard TruSeq
attributes.tag: Experimental Factor: compound
attributes.value: Anisomycin