title: GSM4082158: ZIKV_WT_2; Homo sapiens; miRNA-Seq
library_construction_protocol: total RNA was isolated by Qiagen Rneasy mini kit Library was constructed using QIAseq miRNA Library Kit  (Cat # 331505) by UCLA Technology Center for Genomics & Bioinformatics (TCGB), followed by deep sequenced using Illumina HiSeq3000, as previously reported (Li et al., 2013). Briefly, the workflow consists of 3' ligation, primer hybridization, 5' ligation, first strand synthesis and PCR amplification. Different adapters were used for multiplexing samples in one lane. AMPure XP Beads were used for dual size selection. Library quality was checked with Bioanalyzer (Agilent) and Qubit (Life Technologies).
attributes.tag: GEO Accession
attributes.value: GSM4082158