title: RNA-Seq of Triticum Aestivum
design: PumaXXX and NorstarPuma5A5R were grown and crown tissuesharvested at different stages of cold acclimation. Both genotypes were grown for 14 days (d) at20 o C with a 10h day length. Plants were then treated to decreasing temperatures and daylengthsover a 70d period, designed to mimic field conditions for winter growth habit in Canada. Afterthe initial 14d growth period, the temperature was reduced to 18 o C, then after 3d (15 o C), 7d(12 o C), 14d (9 o C), 21d (6 o C), 28d (3 o C), 35d (2 o C), 42d (2 o C), 49d (2 o C), 56d (2 o C), 63d (2 o C),and 70d (2 o C). In addition to adjusting the temperature, the day length was adjustedincrementally from 13.5h at 0d to to 9.2h at 70d. Day length changes were programmed to occuron day 3 and day 4 of each week. For each change in temperature, crowns were sampled fromtwo independent replicate plants for each genotype, which were used for analysis of geneexpression by RNA sequencing.