title: GSM4291063: Naive_Tomato+LAMP+TRAIL+; Mus musculus; RNA-Seq
library_construction_protocol: Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on TdTomato fluorescence, LAMP1 expression, or TRAIL expression. Post-sort, cells were suspended in 0.1% BSA in 1X PBS. Libraries were created according to the InDrop protocol in Klein et al. Cell 2015 in the Harvard Medical School Single Cell Core according to the standard v3 protocol.
attributes.tag: GEO Accession
attributes.value: GSM4291063