title: GSM4138412: run4: Tail T3; Mus musculus; Bisulfite-Seq
library_construction_protocol: WGBS libraries have been prepared according to Biooscientific's protocol using the Biooscientific NEXTflex™ Bisulfite Library Prep Kit for Illumina Sequencing. Briefly, DNA was fragmented by sonication, size selection was performed using Agencourt AMPure beads XP and adaptators were ligated to be sequenced. Then, bisulfite treatment was performed for 2.5 hours using the EZ DNA Methylation-Gold™ Kit from Zymo Research, and 12 cycles of PCR were performed. Library quality was assessed using an Advanced Analytical Fragment Analyser and libraries were quantified by QPCR using the Kapa Library Quantification Kit. Illumina HiSeq3000 using a paired-end read length of 2x150 pb
attributes.tag: GEO Accession
attributes.value: GSM4138412