title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of prefrontal cortex samples from 11-week old C57BL6/J and DBA/2J mice housed either with a mouse of the same strain or a mouse of the other strain
design: RNA-seq of prefrontal cortex samples from 11-week old C57BL6/J and DBA/2J mice housed either with a mouse of the same strain or a mouse of the other strain
library_construction_protocol: "Mice were euthanized by injection of 250 mg/kg Avertin (20 mg/ml; tribromoethanol) followed by cardiac puncture and exsanguination under deep anesthesia. The brain was dissected and placed in an ice-cold coronal mouse brain matrix. A slab containing the prefrontal cortex (PFC) was removed, and the PFC taken, snap frozen and stored at -80 °C." Mice were co-housed with a single cage mate from either the C57BL6/J or DBA/2J strain from weaning (3 weeks old) to sacrifice (11 weeks old). "Total RNA was extracted using a Qiagen Qiacube and RNeasy mini kit according to the manufacturers protocols and was loaded on a 96-well plate, 17 with samples randomly allocated to wells, for shipment to the sequencing centre." Libraries were prepared according to the dUTP strand-specific protocol.
attributes.tag: Experimental Factor: DBA/2J
attributes.value: strain
attributes.tag: Experimental Factor: had DBA/2J cage mate
attributes.value: environmental history
attributes.tag: Experimental Factor: 2
attributes.value: block