title: GSM4140806: rep5_control; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using a Direct-zol DNA/RNA miniprep kit (R2080, Zymo Research, www.zymoresearch.com) per manufacturer's instructions Fifty nanograms of total RNA was used for each sequencing library. cDNA library preparation was carried out at the Center for Medical Genomics, and included mRNA enrichment using oligo dT-beads to capture polyA RNA, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and amplification, following the KAPA mRNA Hyper Prep Kit Technical Data Sheet, KR1352 – v4.17 (Roche Corporate). Each resulting indexed library was quantified and its quality accessed by Qubit and Agilent Bioanalyzer, and all libraries were pooled at equal molarity. Five microliter aliquots of 2 nM pooled libraries per lane were denatured, neutralized and applied to flow cell deposition on both surfaces of the flow cell; library cluster amplification was performed on a cBot 2 System (Illumina, Inc.). The resulting flow cell was sequenced on an Illumina HiSeq 4000, 75 base paired-end sequencing (Illumina, Inc.).
attributes.tag: GEO Accession
attributes.value: GSM4140806