title: Target sequencing of liver tissue DNA of LAM patients, sample L18
design: We used deep next-generation sequencing (NGS) analysis to identify potential driver genes in LAM. Paired cell-free circulating DNA (cfDNA) and genomic DNA (gDNA) in blood samples were obtained from 23 LAM patients and 7 healthy controls to explore mutated events of targeted 70 cancer-related genes. Somatic mutations of pleural effusions samples and tissue samples of 4 LAM patients were also analyzed.