title: GSM4290648: TJ rep2; Gossypium hirsutum; RNA-Seq
library_construction_protocol: Total RNA was isolated using hot borate (Wan and Wilkins, 1994) followed by column-purification (Zymo Research, Irvine, CA, USA), and mRNA amplified (one-round) with TargetAmp Amplification Kit (Epicenter, Madison, WI, USA).  Amplified mRNA was quantified by bioanalyzer (Agilent, Santa Clara, CA, USA), and 125 ng used to prepare Illumina TruSeq mRNA stranded libraries (Illumina, Inc., San Diego, CA, USA). 125 ng of amplified mRNA was used to prepare Illumina TruSeq mRNA stranded libraries (Illumina, Inc., San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM4290648