title: GSM4290999: Control 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction was performed using TRIZOL reagent (Thermo Scientific, USA), according the manufacturer's instructions. The RNA was quantitated by spectrophotometry using the equipment NanoVue (GE Healthcare Life Sciences, USA). RNA Integrity was ensured by obtaining RNA Integrity Number - RIN > 8 with Agilent 2100 Bioanalyzer (Agilent Technologies, Germany). RNA samples were treated with DNA Free Kit (Thermo Scientific, USA) to remove genomic DNA contamination. RNA sequencing libraries were constructed with TruSeq Strandes Total RNA Sample Prep Kits (Illumina, USA) using 1000 ng total RNA. Samples were indexed with adaptors and submitted for paired-end 2 × 100-bp sequencing using an Illumina HiSeq 2000 instrument.
attributes.tag: GEO Accession
attributes.value: GSM4290999