title: 16S metagenome of macaca mulatta:stool
design: Sequencing of the V3-V4 region of the bacterial 16S rRNA gene was performed using the 515FB-806RB primer set, per Earth Microbiome Protocols. Amplified in triplicate for each sample followed by pooling of 3ng of each replicate. The pooled library was cleaned using QIAQuick PCR Purification kit and quantified using the KAPA Library Quantification Kit. Sequencing was carried out as detailed in the Earth Microbiome Project protocol; specifically, 7 pM of the pooled library with 30% PhiX phage as a control was sequenced using a 300-cycle Illumina MiSeq Kit, generating 150bp paired-end reads.