title: Metagenomics of Human vaginal microbiome: Sample PY18
design: "Vaginal specimens in the ESwabTM transport kits were stored at -20degrees Celsius. DNA was extracted and PCR was performed with unique bar coded primers to amplify the V4 region of the 16S rDNA. The samples were first quantitated using Pico Green, adjusted to a concentration of 4nM then used for sequencing on the Illumina MiSeq. Paired-end reads of approximately 250 bp were sequenced."