title: Ant_Transcriptom: Apterostigma
design: 5 Specimens of single colony of A. megecephala were sampled live and preserved in RNAlater, and crushed with sterilized wooden sticks, and then stored at -20°C until RNA extraction. RNA was extracted using the Promega SV Total RNA Isolation System, following the standard protocol. The Institute for Bioscience and Biotechnology Research (IBBR) at the University of Maryland prepared libraries using the standard Ilumina TruSeq RNA Sample Preparation protocol (all libraries were normalized and redundant rRNA was removed), and performed 100bp pair-end sequencing on Ilumina HiSeq.