title: ddRADseq of Aedes aegypti: larvae
design: Library creation followed the ddRADseq preparation method outlined                 in Peterson 2012, "Double Digest RADseq: An Inexpensive Method for De Novo SNP                 Discovery and Genotyping in Model and Non-Model Species", as follows: 81 mosquito                 larvae had genomic DNA individually extracted using the Roche Hi Pure preparation                 process. These samples were double-digested with the restriction enzymes NalIII and                 MluCI, barcodes were ligated to the fragments, then the samples were pooled together                 before undergoing size selection (350 - 450 bp), then a PCR enrichment step.                 Sequencing occurred with a 2-% Phi-C spike-in to reduce the impact of low-diversity                 RADtags.