title: RNA-seq of Sparus aurata: juvenile male brain
design: The sample preparation was performed according to the protocol NEBNext Ultra Directional RNA Library Prep Kit for lllumina. mRNA was isolated using the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. The quality and yield after adapter ligation and PCR, was measured with the Fragment Analyzer. Clustering and DNA sequencing using the lllumina cBot and HiSeq 2500 was performed, respectively.