title: Illumina HiSeq 2000 paired end sequencing; Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)
design: Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)
library_construction_protocol: Total RNA was purified by RNeasy Minikit (Qiagen) according to the manufacturer's manual. RNA concentration was determined by Nanodrop, and 500 ng of total RNA was used for library construction using KAPA Stranded mRNA-Seq Kits. Sequencing was performed on Illumina HiSeq 2000 machines with 75 bp pair-end mode. RNeasy Minikit (Qiagen)  KAPA Stranded mRNA-Seq Kits
attributes.tag: Experimental Factor: organism
attributes.value: Homo sapiens
attributes.tag: Experimental Factor: developmental stage
attributes.value: Trophoblast
attributes.tag: Experimental Factor: cell line
attributes.value: Man 1
attributes.tag: Experimental Factor: growth condition
attributes.value: TSC media