title: Colorado potato beetle larval transcriptome
design: Total RNA was extracted from 142 Colorado potato beetles larvae, in all instar stages, from four European populations. RNAs were used for ds_cDNA synthesis using the SMART approach. SMART-prepared amplified cDNAs were pooled and normalized using the DSN normalization method to reduce overabundant transcripts. Normalization included cDNA denaturation/reassociation, a treatment by duplex-specific nuclease and the amplification of the normalized fraction by PCR.  Approximately 20 *g of normalized cDNA were used for the sequencing libraries construction at BMR Genomics, Padova, Italy. The sequencing was performed on a half plate in a 454 GS-FLX titanium series pyrosequencer (Roche Applied Science).