title: SMRT-seq of Undaria pinnatifida
design: The mRNA was isolated from the pooled total RNA with oligo (dT) magnetic beads (InvitrogenTM Life Technologies, Carlsbad, CA, USA) and reverse transcribed into cDNA using SMARTer PCR cDNA Synthesis Kit (Clontech, CA, USA). Double-stranded cDNA was generated by using the optimized PCR cycle number. BluePippinTM Size-Selection System (Sage Science, Beverly, MA, USA) was employed for size selection of the double-stranded cDNA with three size ranges (1-2 kb, 2-3 kb, 3-6 kb). Another amplification was performed for the SMRTbell library construction.