title: GSM4569755: PAO1_serum-azm_2; Pseudomonas aeruginosa PAO1; RNA-Seq
library_construction_protocol: Sixty minutes post-treatment, bacterial cells were collected and mixed 1:2 volume wise with RNA protect Bacteria Reagent (QIAGEN). RNA was extracted using RNeasy mini kit (QIAGEN). RNA was quantified using a Nanodrop-1000 (ThermoFisher). RNA quality and quantity were assessed on an Agilent Bioanalyzer. rRNA was depleted (Ribo-Zero depletion kit, Illumina), and cDNA libraries were prepared using the KAPA RNA HyperPrep Kit (KAPA Biosystems) and sequenced through the University of British Columbia Sequencing Consortium (Illumina HiSeq-2500)
attributes.tag: GEO Accession
attributes.value: GSM4569755