title: GSM4138719: NDN_03_LE [RNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood was collected by heparinized tubes and the PBMC fraction was obtained using Ficoll/Hypaque gradient. Bulk LDGs were isolated by magnetic bead negative selection. CD10+ LDGs, CD10- LDGs, and CD10+ NDN were sorted by flow cytometry using CD10-APC,CD14-FITC and CD15-PE antibodies. RNA was isolated using Zymo Research Direc-Zol RNA Miniprep kit. cDNA libraries were prepared using the TruSeq® Stranded mRNA NeoPrep™ Kit (Illumina). RNA seq data were generated on Illumina HiSeq 3000 instruments according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4138719