title: RNA-seq of Pinus sylvestris: Zabor'e 3
design: Pine needles from the four cardinal orientations belonging from three different individuals with estimated age between 20-25 years old were harvested and frozen in liquid nitrogen. Total RNA was extracted with GeneJET RNA Purification Kit (Thermo Fisher Scientific), following cDNA synthesis with Mint-2 kit (Evrogen), library preparation with NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs), and double bar-coding of the libraries with NEBNext Multiplex Oligos for Illumina (New England Biolabs). The samples were sequenced as 100 bp paired-end reads with an Illumina HiSeq2500 platform by Genotek (Moscow, Russia).