title: GSM2429208: Contact_Fixed_1; Zea mays; RNA-Seq
library_construction_protocol: RNA was isolated using the ZR Plant RNA MiniPrep™ kit (Zymo Research). Tissues were homogenized using a FastPrep®-24 (MP Biomedicals), twice for 30 s at 6.0 m/s. On-column DNase digest was done using RNase-Free DNase Set (50) kit (QIAGEN). Ribosomal RNA was removed using the RiboZero™ Magnetic Kit for Plant Seed/Root (Epicentre), followed by cleanup using the Zymo RNA Clean & Concentrator™-5 kit (Zymo Research). Sequencing libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM2429208