title: GSM3439978: WT rep1 liver RRBS; Mus musculus; Bisulfite-Seq
library_construction_protocol: A total of 18 DNA samples were isolated from gonadal adipose, hypothalamus and liver tissues from male offspring using the AllPrep DNA/RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). The DNA samples were quantified using the dsDNA BR assay (Qubit, Waltham, MA) and 100ng of DNA was used for library preparation. After digestion of the DNA with the MspI enzyme, samples underwent an end-repair and adenylation process, followed by adapter ligation using the Truseq barcode adapter (Illumina, San Diego, CA), size selection using AMPure Beads (Beckman Coulter, Brea, CA), and bisulfite treatment using the Epitect Kit (Qiagen, Germantown, MD). Bisulfite-treated DNA was then amplified using the Truseq Library Prep Kit (Illumina, San Diego, CA) and sequenced with the Illumina Hiseq2500 System.
attributes.tag: GEO Accession
attributes.value: GSM3439978