title: GSM1143759: FA19mtr120 3; Neisseria gonorrhoeae FA19; RNA-Seq
library_construction_protocol: Total RNA was extracted from 1 mL culture samples using the Ambion RiboPure Bacteria kit according to manufacturer’s instructions. DNA was removed using Ambion TURBO DNA-free kit according to manufacturer’s instructions. rRNA was depleted by two treatments with Ambion MICROBExpress according to manufacturer’s instructions. cDNA libraries were prepared using the Invitrogen SuperScript Double-stranded DNA synthesis kit and random hexameric primers according to manufacturer's instructions. cDNA librariers were amplified by 15 cycles of PCR to add adaptors with TruSeq indexes using the TruSeq Sample Preparation kit according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM1143759