title: GSM4216238: invitro_ko_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Cell pellets were harvested and RNA extraction was performed with the RNeasy Mini Kit (Qiagen) according to manufacturer's instruction and using in-column DNase digestion. The NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB) was used according to manufacturer's protocol to purify Poly-A+ RNA from total RNA. RNA extraction was performed with the RNeasy Mini Kit (Qiagen) according to manufacturer's instruction and using in-column DNase digestion. The NEBNextPoly(A) mRNA Magnetic Isolation Module (NEB) was used according to manufacturer's protocol to purify Poly-A+ RNA from total RNA. RNA quality was assessed employing a Fragment Analyzer (Agilent) and cDNA libraries were generated using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4216238