title: 16S-Tag: Prokaryotes_T24_ephyrae_r2
design: PCR amplification of 16S rRNA v4 with S-*-Univ-0515-a-S-19_UNI1 forward primer and mix of reverse primers S-D-Bact-0785-b-A-18_UNI2 and S-D-Bact-0787-b-A-20_UNI2 (in equal concentration). Sequencing adapters, barcode, PCR primers have been removed. Sequence shorten than 150 bases and with an expected error higher than 1 nucleotide each 100 bases were discarded.