title: PacBio sequencing of E. coli strain C600
design: Single Molecule, Real-Time (SMRT) sequencing was performed on the Pacific Biosciences (PacBio) RSII sequencing platform using PacBio SMRTbell Template Prep kit 1.0., with the P6/C4 sequencing chemistry and 360-min data collection protocols. DNA was extracted using 100/G columns (Qiagen), sheared using a Corvaris g-tube, and libraries were prepared from 10 mg of bacterial genomic DNA, using the standard protocol, and processed for sequencing as recommended by the supplier.