title: Illumina MiSeq sequencing; qiita_ptid_4749:10719.K15F
design: bacterial kinetics in early life (first weeks)
library_construction_protocol: DNA from total intestinal tissue was isolated with the QIAamp DNA Stool Mini Kit (QIAGEN) as instructed by the manufacturer with additional bead beating steps. For in-depth microbiota profiling, 16S rRNA gene V4 region amplicons from fecal DNA isolates were amplified by the 515F/806R primers and sequenced on an Illumina MiSeq platform using 2x300 bp according to established protocols (1).
attributes.tag: barcode
attributes.value: TCTTGGAGGTCA
attributes.tag: center_name
attributes.value: Uniklinik RWTH Aachen
attributes.tag: linker
attributes.value: GT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4