title: soil_fungal_amplicon_E_c_plot
design: DNA extracted from pulverized soil with MoBio Power soil kit. Amplicons produced with tagged-ITS4 and fITS7 primers. Triplicate 20 ul reactions per sample done with 10 ng/ul template DNA, 2  M of each primer, 0.2 mM dNTP s, 1U Phusion High-Fidelity polymerase and 1   HF buffer. PCR conditions: initial denaturation at 98 C for 1 min; 27 cycles of 98 C for 10 sec, 57 C for 20 sec, 72 C for 30 sec, and a final extension at 72 C for 7 min. After PCR triplicate amplicons pooled and cleaned with AMPure XP  and quantified with Bioanalyzer.