title: GSM4291373: Runx3_P1_CD8_4Cseq_rep2; Mus musculus; OTHER
library_construction_protocol: ChIP DNA was extracted using the Phenol:Chloroform extraction followed by QiaQuick PCR Purification kit using standard protocol. RNA was extracted using TriZol reagent and ethanol precipitation. CapStarr-seq RNA was extracted as described by Vanhille et al. 2015 (Nature Communications). 4C material was extracted as described by Ben Zouari et al. 2020 (Frontiers in Genetics) ChIP-seq libraries were prepared with Illumina's ChIP Sample Prep Kit according to standard protocol. RNA-seq Libraries were prepared either with ScriptSeq V2 Total RNA Library Prep Kit (EpiCenter, USA) by using manufacturer's recommended protocol. 4C libraries are generated directly during the extraction process.
attributes.tag: GEO Accession
attributes.value: GSM4291373