title: Illumina HiSeq 2000 sequencing; Effects of cycloheximide (CHX) on ribosome profiling experiments in Schizosaccharomyces pombe
design: Effects of cycloheximide (CHX) on ribosome profiling experiments in Schizosaccharomyces pombe
library_construction_protocol: Cells were grown at a temperature of 32C in EMM2 (Moreno et al. Methods Enzymology 194: 795) containing NH4Cl as a nitrogen source Total RNA was extracted using a hot phenol extraction method as described in Lyne et al. (2003) BMC Genomics 4:27. Clean up and DNase treatment were performed on with Purelink RNA microcolumns (Life Technologies) following the manufacturer’s instructions.  Samples were ribo-depleted using Ribo-Zero Gold rRNA Removal Kit Yeast (Illumina) with a 4 ug input.   30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).
attributes.tag: Experimental Factor: compound
attributes.value: Cycloheximide
attributes.tag: Experimental Factor: growth condition
attributes.value: Nitrogen source
attributes.tag: Experimental Factor: RNA source
attributes.value: Ribosome-depleted RNA