title: GSM4007900: 1051 edge9 [OtC1095]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using the TRIzol Plus RNA Purification System (Invitrogen). RNA integrity was checked by Agilent Bioanalyzer 2100, only samples with clean rRNA peaks were used.  Libraries for RNA-seq were prepared according to KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems, Wilmington, MA) system. Final library quality and quantity were analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit3.0 Fluorometer, respectively. 150 bp Paired-end sequencing was performed on Illumina HiSeq 4000 (Illumnia Inc., San Diego, CA).  RNA integrity was checked by Agilent Bioanalyzer 2100, only samples with clean rRNA peaks were used.  Libraries for RNA-seq were prepared according to KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems, Wilmington, MA) system. Final library quality and quantity were analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit3.0 Fluorometer, respectively. 150 bp Paired-end sequencing was performed on Illumina HiSeq 4000 (Illumnia Inc., San Diego, CA).  RNA integrity was checked by Agilent Bioanalyzer 2100, only samples with clean rRNA peaks were used.  Libraries for RNA-seq were prepared according to KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems, Wilmington, MA) system. Final library quality and quantity were analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit3.0 Fluorometer, respectively. 150 bp Paired-end sequencing was performed on Illumina HiSeq 4000 (Illumnia Inc., San Diego, CA).  RNA integrity was checked by Agilent Bioanalyzer 2100, only samples with clean rRNA peaks were used.  Libraries for RNA-seq were prepared according to KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems, Wilmington, MA) system. Final library quality and quantity were analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit3.0 Fluorometer, respectively. 150 bp Paired-end sequencing was performed on Illumina HiSeq 4000 (Illumnia Inc., San Diego, CA).
attributes.tag: GEO Accession
attributes.value: GSM4007900