title: RNA-seq of Phomopsis longicolla hap3 mutant during seed colonization
design: Phomopsis longicolla wild-type and hap3 mutant were inoculated on soybean seeds of cultivar Traff. Each strain had three 24-seed replicates. In order to enrich samples for P. longicolla tissue, six days after inoculation seed coats were carefully removed from colonized seeds, pooled by sample, flash frozen and manually ground in liquid nitrogen, and total RNA was extracted with Ribozol (Amresco, Solon, OH, USA).