title: GSM1142599: RNA in the male germ cells at 15.5 dpc; Mus musculus; RNA-Seq
library_construction_protocol: GFP+ germ cells and GFP- gonadal somatic cells were flow sorted based on Oct4 promoter-driven EGFP expression in germ cells from CF1XOG2 embryo and fetus gonads using a MoFlo or Aria II flow cytometer. Total RNA was extracted using RNA Bee (Tel Test Inc.). Contaminating DNA was removed with the DNA-free Kit (Ambion). The amount of RNA was monitored using a Nanodrop spectrophotometer followed by a quality check using the Bioanalyzer. All RNA samples had a RIN>9.0. 2µg total RNA samples were depleted for rRNA using the RiboZero Kit (Epicenter, Illumina). RNA-seq libraries were prepared and paired-end sequencing was performed using the IntegenX RNA kit (Illumina) with read length/coverage of 2x100.
attributes.tag: GEO Accession
attributes.value: GSM1142599