title: GSM4683914: BRA79; Homo sapiens; RNA-Seq
library_construction_protocol: Minor salivary glands were obtained from study participants and stored in RNAlater (Qiagen) until RNA extraction. Samples were homogenized with a Bullet-Blender (Next Advance) or an Omni TH (Omni International). Total RNA was extracted with an RNeasy Mini kit according to the instructions of the manufacturer (Qiagen). The quality of RNA was measured using a 2100 Bioanalyzer (Agilent). Only RNA samples with a 28S/18S ribosomal RNA ratio of 1.7 and an RNA integrity number of ≥ 6.5 were used for the arrays. The cDNA libraries were produced by the Genomics and Computational Biology Core (GCBC) using the Nextera XT method.
attributes.tag: GEO Accession
attributes.value: GSM4683914