title: tRNA-Seq of human: Skin/Dermal fibroblast.: Sample trna2.Aff1.NSun2.2hr_stress.3
design: "Human dermal fibroblasts lacking NSUN2 were infected with p-Babe expressing empty vector, wt-NSUN2 or NSUN2-K190M. They were either untreated or treated with sodium arsenite for 2 or 4 hours. Total RNA was extracted using Trizol and treated with TURBO DNAse. RNA samples were size selected on TBE Urea gels for 20-150bp fragments. Samples were treated with T4 PNK for library preparation using an Illumina TruSeq Small RNA kit."