title: targeted sequencing capture of sea otter historical tooth root samples
design: Targeted probes were designed to capture ~60Mb of sequence from the sea otter genome, including exons, neutral regions >10kb from genes, and 1kb upstream of all genes. Blood and tissue samples were extracted using Qiagens DNEasy Blood and Tissue kit. Sequence libraries were generated and subjected to sequence capture following the Kapa SeqCap EZ Hyperflow workflow. Sample was subjected to serial sequence capture to further enrich for endogenous content, resulting in high levels of PCR duplicates. Coverage of target regions is ~2.3X.