title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of E14Tg2a cells upon Zic3 knockdown after day1 and day2 of differentiation into epiblast-like stem cells
design: RNA-seq of E14Tg2a cells upon Zic3 knockdown after day1 and day2 of differentiation into epiblast-like stem cells
library_construction_protocol: cell culture cell culture 12ng/ml bFGF, 20ng/ml activin A, 1% KOSR on 5ug/ml bovine plasma fibronectin-coated dishes  RNAeasy Plus Mini kit Illumina TruSeq RNA library prep kit v2
attributes.tag: Experimental Factor: time
attributes.value: 1
attributes.tag: Experimental Factor: RNA interference
attributes.value: Zic3 knockdown by siRNA