title: GSM4010649: WT1_24h; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from 500,000 HCT116 cells  using TriFast reagent  (peqlab) according to manufacturer's extraction. Small RNAs (< 200 nt) were discarded from total extracted RNA with Zymo-spin IC columns (RNA Clean and Concentrator-5, Zymo Research). Total RNA (1 µg) was subjected to rRNA depletion as described  (Kim et.al, 2019, doi: https://doi.org/10.1101/670604 ). RNA libraries from 1 µg of total RNA were constructed as described (Zhang et al., 2012).