title: GSM4083956: Sham_sfa.eaa.rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using RNeasy Mini Kit (Qiagen), and treated with DNase according to the manufacture's protocol. Sample Validation:  -Standard RNA Reagent Kit (Perkin Elmer – Cat # CLS960010)  -HT DNA 5K/RNA/CZE LabChip (Perkin Elmer – Cat # 760435)  -LabChip GXTouch HT Instrument (Perkin Elmer)     -Sample Quantification  Qubit® RNA Assay Kit (LifeTechnologies – Cat# Q32852)  QUBIT 3.0 Fluorometer Instrument (LifeTechnologies – Cat# Q33216)     -Library Generation  TruSeq® Stranded mRNA Sample Prep Kit HT - (Illumina - Cat# RS-122-2103)   Purify and Fragment mRNA  Synthesize First/Second Strand cDNA  Adenylate 3' Ends  Ligate Adapters  - PCR Amplification  Plus: Agencourt AMPure XP (Beckman Coulter - Cat# A63881)    -Library Validation  HT DNA High Sens Reagent Kit (Perkin Elmer – Cat # CLS760672)  HT DNA 1K/12K/HI Sens Chip (Perkin Elmer – Cat # 760517)  LabChip GXTouch HT Instrument (Perkin Elmer)  Qubit® dsDNA HS Assay Reagent Kit (LifeTechnologies – Cat# Q32851)  Qubit® Assay Plastic Tubes (LifeTechnologies – Cat# Q32856)  QUBIT 3.0 Fluorometer Instrument (LifeTechnologies – Cat# Q33216)    -Normalization and Pool    -Denaturation and Dilution of Libraries    -Sequencing  NextSeq® 500/550 High Output v2 Reagent Kit (150 cycles) – Illumina – Cat# FC-404-2002)  NextSeq 500 Instrument
attributes.tag: GEO Accession
attributes.value: GSM4083956