title: GSM1138347: degradome-orange-Fr; Citrus sinensis; RNA-Seq
library_construction_protocol: All samples were collected and frozen in liquid nitrogen immediately after collection and kept at -80℃ until use.Total RNA was extracted using Trizol reagent. The 3’ fragment generated by RISC (RNA-induced silencing complex), which contains a 5’-monophosphate, was ligated to a 5’ RACE adaptor. Reverse transcription is then performed using oligo(dT) as a 3’ adaptor and the cDNAs were formed by second strand synthesis.
attributes.tag: GEO Accession
attributes.value: GSM1138347