title: GSM4290693: TX701 TRV:GhSP rep2; Gossypium hirsutum; RNA-Seq
library_construction_protocol: At 15 days post-inoculation, apices were excised from inoculated seedlings and uninoculated controls, fixed in acetone with vacuum-infiltration, and acetone changed twice before storing at 4°C. Acetone-dried apices were trimmed to 5 mm and expanding leaves were removed. Dried samples were frozen in liquid nitrogen, homogenized using a Retsch mill (Retsch GmbH, Haan, Germany), and RNA isolated by hot borate (Wan and Wilkins, 1994) followed by column clean-up (Zymo Research, Irvine, CA, USA). 2 µg of total RNA from uninoculated, dCLCrV:GhSFT-, and TRV:GhSP-infected DP61 and TX701 plants were used to prepare Illumina TruSeq Stranded mRNA libraries (Illumina, Inc., San Diego, CA, USA) as per the manufacturer's protocols, with three biological replicates per treatment per accession.  Libraries were sequenced on a HiSeq2000 (the University of Texas Southwestern Genomics Core), and >30 million 50 bp single-end reads were obtained per replicate.
attributes.tag: GEO Accession
attributes.value: GSM4290693