title: mice gut microbiota
design: This study was conducted to characterize the mice intestinal microbiota at different feeding conditions includes: the mice were fed standard diet (CTL), diet containing 45%Kcal fat diet (HF) and HF containing 1% laminarin (HFL) for four weeks. The bacterial community structure was analyzed based on 16S rRNA sequences (V4 region, 253 bp). Total DNA was extracted from 150 mg of fecal materials using MOBIO Power Fecal DNA isolation kit (MO BIO Laboratories Inc., CA USA). PCR was carried out in triplicate using Maxime PCR PreMix Kit (iNtRON Biotechnology Inc., Korea). Amplicons were extracted from agarose gel using the AccuPrep Gel Purification kit (Bioneer Inc., Korea). All obtained DNA was quantified using Qubit (Invitrogen, CA, USA), and equimolar purified amplicons were pooled, ligated adapters for Illumina MiSeq sequencing according to MiSeq protocols (Illumina Inc., USA).  MiSeq Sequence adapters, unique molecular identifier (MID) tags, pads and linkers were incorporated at the 5’ end of both target-specific primers to allow identification of individual sequences from pooled amplicons (Forward primer: 5’-GTGTGCCAGCMGCCGCGGTAA-3’; Reverse primer: 5’-CCGGACTACHVGGGTWTCTAAT-3’).
attributes.tag: loader
attributes.value: latf-load
attributes.tag: quality
attributes.value: p