title: Illumina HiSeq 2000 sequencing; qiita_ptid_24:1222.B4.5.8.06
design: Water samples were obtained from a replicated mesocosm study (two treatments, each in triplicate) established in coastal waters of a fjord close to Bergen, Norway (60.27uN: 5.22uE). Each mesocosm contained 11,000 L of coastal water and two of the six mesocosms were sampled for this study. To induce the phytoplankton bloom, nitrate and phosphate were added. Water samples were taken at the peak and immediately following the collapse of the phytoplankton bloom from both a high CO2 and control mesocosm. Water samples were collected in May 2006. Metagenomic and Metatranscriptomics data has already been published. These data presented here are 16S rRNA V3-V4 Illumina amplicon data.
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: GTCGCCGTACAT
attributes.tag: center_name
attributes.value: ANL
attributes.tag: center_project_name
attributes.value: Bergen Ocean Acidification Mesocosms
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: MiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: ANL
attributes.tag: run_date
attributes.value: 6/2/11
attributes.tag: run_prefix
attributes.value: Mesocosm-pool_110602_HWI-EAS137R_0375_s_8_1_sequence
attributes.tag: samp_size
attributes.value: .1,g
attributes.tag: sample_center
attributes.value: ANL
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4