title:  30 x whole genome DNA sequence for NHLBI TOPMed sample NWD234188 
design:  PCR-free library prep uses randomly sheared DNA (Covaris, targeting 385 bp fragments) and palindromic forked adapters containing unique 8 base index sequences with a commercially available reagent kit (KAPA BioSystems product KK8202).  Barcoded samples are pooled in groups of 24 samples each and each pool is sequenced on three flow cells of HiSeq X. 