title: Clostridium thermocellum AD2 PacBio Sequencing
design: Genomic DNA underwent Pacific Biosciences standard template preparation and sequencing protocols. Library preparation selected ~10 kb fragments for strain AD2 using Covaris g-Tubes and AMPure PB bead-based size selection. SMRTbell template libraries were sequenced on a Pacific Biosciences RSII sequencer with sequencing version C2 chemistry.