title: RNA-seq of Vinclozolin F2 rat total RNA with 3 replicate
design: The F3 generation vinclozolin and control lineage male epididymal sperm were collected and processed as previously described and stored at -70 C until use (44).  The total RNA was isolated using the mirVana miRNA Isolation Kit (Life Technologies) following the manufacturer s instructions with modifications at the lysis stage. In brief, after addition of lysis buffer, the frozen sperm pellets were homogenized at low settings for 90 seconds, followed by a five-minute incubation at 65  C.  Samples were then placed on ice, and the default protocol was resumed.  For quality control, RNA integrity numbers (RIN) were obtained by RNA 6000 Nano chips run on an Agilent 2100 Bioanalyzer (Agilent). A RIN of 2   4 indicates good sperm RNA quality.