title: miRNA-Seq of Xenopus laevis: Egg pool
design: Libraries were generated according to the manufacturers’ protocols using the Ion Total RNA-Seq Kit v2 and the Ion Xpress™ RNA-Seq bar coding kit (Thermo Fisher Scientific). A few modifications were made during purification steps in the small-RNAseq to allow sequencing of longer RNAs (up to 200 nt) than with standard sRNA protocols. Namely: 153 µl ethanol instead of 120 µl during purification of cDNA and 134 µl ethanol instead of 110 µl in protocol.