title: A24-3-16S
design: Bacterial 16S rDNA V4 region was amplified by primer pair 515F-806R, and fungal ITS1 region was amplified by primer pair ITS5-1737F.sequencing libraries were generated using TruSeq DNA PCR-free Library Prep Kit from Illumina (NEB, USA) following manufacturer s protocol, and index codes were added.