title: GSM2425740: HP1a_HMRRNAi_1; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: For chromatin immunoprecipitation (ChIP) cells were crosslinked with 1 % formaldehyde for 5 min at room temperature. Upon cell lysis, protease inhibitors and proteasome inhibitor MG-132 (Enzo Life Sciences) were applied. The chromatin was isolated and sheared with adaptive focused acoustics (Covaris) to an average size of 200 base pair (bp). For each ChIP reaction, chromatin isolated from 1-2 x 10e6 cells was incubated with antibodies precoupled to Protein A/G Sepharose. All libraries were prepared using MicroPlex (Diagenode) or NEBNext (NEB) Library Preparation kit.
attributes.tag: GEO Accession
attributes.value: GSM2425740