title: GSM3439912: Het rep3 (scRNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: Mice were immunized with MOG. TCRb+YFP+ cells from spleen at day 9 post-immunization sorted on an iCyt Reflection cell sorter. The cells were counted and examined for viability using a Luna Dual Florescence Cell Counter (Logos Biosystems). All samples were spun down at 2,000 rpm for 5 min. The supernatant was removed, and cells were re-suspended in 100 ul of 1× PBS (Thermo Fisher Scientific) + 0.04% BSA (Amresco) Single-cell suspensions were loaded onto the Chromium Controller according to their respective cell counts to generate 6000 single cell  GEMs (gel beads in emulsion) per sample. Libraries were prepared using the Chromium Single Cell 3' v2 Library and Gel Bead Kit (10x  Genomics)
attributes.tag: GEO Accession
attributes.value: GSM3439912