title: 16S rDNA amplicon from soil DNA
design: primers S-D-Bact-0341-a-S-17 (5-CCTACGGGAGGCAGCAG-3) and S-D-Bact-0787-b-A-20 (5-GGACTACNVGGGTWTCTAAT-3; Muyzer et al., 1993; Caporaso et al., 2010) using dual-index paired-end strategy (Kozichetal.,2013)