title: DNA from a healthy female
design: The control sample was diluted to around 20 million molecules. This means that each molecule has a unique starting position in the genome, i.e. it is unique with a high probability. The mixed libraries were then amplified and sequenced until saturation that is each molecules is seen preferably at least two times. It is then possible to bioinformatically remove molecule copies. The idea is that all or most molecules before amplification should be seen after sequencing, and the bias from amplification should be removed.  The Mixed The control sample was sequenced one two lanes on the Illimina HiSeq 2000.  All unique 45 bp long sequences were extracted from the hg19 genome with Bowtie 1.0, and used as a reference genome. The reads were aligned to this reference genome with a custom program that doesn’t allow mismatches, deletions or insertions. A bam file instead of fastq file was uploaded to SRA to save space.