title: Hi-C rep2
design: Construction of Hi-C library was performed by as described before (Wang et al. 2015) using DpnII as the restriction enzyme for chromatin fragmentation. For one round of preparation, nuclei extracted from 2 g fixed tissues were equally divided into 5 tubes and preceded in parallel. After biotin labeling of chromatin fragment ends and heat inactivation of DpnII, all 5 tubes were continued to the ligation step. Subsequent chromatin DNA manipulations were performed according to (Wang et al. 2015). The final library molecules were sequenced on Illumina HiSeq 2000 with 2x101 bp reads.