title: GSM4138198: HIV_Pos_1_106_PBMCS; Homo sapiens; RNA-Seq
library_construction_protocol: For work on unsorted cells, total RNA was extracted from RNAlater-stored cellular pellets using Qiagen RNeasy Micro kit (Qiagen, Hilden, Germany). For work on purified CD8+ T cells, cells were sorted into 70% TRIzol LS Reagent (Thermo Fisher Scientific, Waltham, MA, USA)  followed by isolation of both RNA and DNA using the TRIzol/chloroform method. Total RNA was then extracted using Qiagen RNeasy Micro kit. Enrichment for messenger RNA was done using the NEBNext Poly(A) mRNA Magnetic Isolation kit (New England Biolabs, Ipswich, MA, USA). RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) in accordance to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4138198