title: Whole exome sequencing for case A1: Sample A1
design: Whole exome libraries are constructed as described by (Fisher et al. 2011 Genome Biology) with some slight modifications. Initial genomic DNA input into shearing was reduced from 3µg to 50ng in 10µL of solution and enzymatically sheared. In addition, for adapter ligation, dual-indexed Illumina paired end adapters were replaced with palindromic forked adapters with unique 8 base index sequences embedded within the adapter and added to each end.