title: Illumina HiSeq 2000 sequencing; Posttranscriptional mRNA expression changes in stress
design: Posttranscriptional mRNA expression changes in stress
library_construction_protocol: Cells were seeded 48 hours prior to treatment. DMSO was subsequently added to cells  as a solvent control. Cells were harvested in PBS 3 hours later. Total RNA was extracted using Trizol LS. Cells were fractionated and nuclear RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina TruSeq protocol.
attributes.tag: Experimental Factor: Extraction protocol
attributes.value: nuclear
attributes.tag: Experimental Factor: Nucleic acid library construction protocol
attributes.value: standard TruSeq
attributes.tag: Experimental Factor: compound
attributes.value: DMSO