title: GSM4683657: D21 EAE Atg7 KO P9210_132; Mus musculus; RNA-Seq
library_construction_protocol: CNS cells were extracted using Neural Tissue dissociation kit T (Miltenyi Biotech) and sorted using a BD Influx cell sorter. Microglia were sorted as Live, CD11b+ CD45Intermediate(Int) Ly6G- and/or eYFP+. Sorted microglia were lysed in RLT betamercaptoethanol and pooled 1:1 female and male. RNA was prepared using an RNeasy Mini Kit (Qiagen) followed by quality control assessed with a Bioanalyzer 2100 (Agilent). All samples included had high quality RNA (RIN = 8.5–10). RNA was amplified with a SMARTer Stranded Total RNA-Seq Kit–Pico Input Mammalian (Clontech). RNA libraries were prepared for sequencing using the SMARTer Stranded Total RNA-seq Kit - Pico Input Mammalian (Clontech).
attributes.tag: GEO Accession
attributes.value: GSM4683657