title: GSM4291999: 965-M2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from samples of ~1000 migratory or unsorted cells using the RNeasy Micro Kit (Qiagen). RNA was evaluated by Bioanalyzer using the Agilent RNA 60000 Pico kit. Samples with RNA Integrity Number (RIN) 8.4-9.3 were used. 335pg of RNA of each sample were used to prepare complementary DNA (cDNA) libraries using the Smart-seq v4 kit (Takara). cDNAs were purified by AMPure XP beads, fragmented by sonication to ~400bp, and subjected to barcoding and single-end sequenced on Nextseq 500 with 75 cycles. Raw sequencing depth ranged 38.7 – 76.1 million reads with >97.2% of reads mappable to the human genome.
attributes.tag: GEO Accession
attributes.value: GSM4291999