title: GSM1139102: Primary MCL #4; Homo sapiens; RNA-Seq
library_construction_protocol: For each experimental condition, 100 ng of high quality total RNA (RIN > 0.8 on the Agilent BioAnalyzer 2100) was isolated using the RNAEasy kit according to the manufacturer’s instructions (QIAGEN) All RNAs were converted to cDNA, isolated with magnetic beads from the TruSeq mRNA prep kit (v2), and then ligated to Illumina adapters, as per the standard TruSeq Illumina protocol. Using these multi-plexed cDNA libraries, we generated clusters on the Illumina cBot station and paired-end sequenced each sample to 50x50 bp on the Illumina HiSeq2000 at the Weill Cornell Medical College (WCMC) Epigenomics Core
attributes.tag: GEO Accession
attributes.value: GSM1139102