title: GSM2428813: Xenopus_laevis_RNAseq_stage10.5_replicate1; Xenopus laevis; RNA-Seq
library_construction_protocol: Total RNA was extracted from 20 Nieuwkoop-Faber stage 10.5 embryos (two biological replicates) using Trizol and Qiagen columns. 4-5 ug of total RNA was treated with DNase I on column and depleted of rRNA (ribosomal RNA) using Magnetic gold RiboZero RNA kit (Illumina) resulting in a yield of 45 - 52 ng of rRNA depleted total RNA. 2 ng of rRNA-depleted total RNA was reserved for Experion (Bio-Rad) quality assessment run for rRNA depletion and the remaining was used for first and second strand synthesis (strand-specific protocol). Total yield of dscDNA was between 14.5-15.8 ng and out of this 1.2 - 5 ng was used for sample preparation for high high-throughput sequencing (according to manufacturer's protocol).
attributes.tag: GEO Accession
attributes.value: GSM2428813