title: GSM4289841: 25-Mcln-1-T1-E2; Mycobacteroides chelonae; RNA-Seq
library_construction_protocol: In four independent experiments, cultures of M. chelonae were diluted in Sauton's media to an OD of 0.03 and grown in a 75cm2 cap-vented culture flask, in a 30 degrees C static incubator for 5 (t1) or 10 (t2) days. After harvesting at 5 or 10 days, cells pellets were spun down and immediately frozen at -80 degrees C until RNA isolation . Cell pellets in TRIzol were transferred to a tube containing Lysing Matrix B, and vigorously shaken at max speed for 30 s in a FastPrep 120 homogenizer three times. This mixture was centrifuged at max speed for 1 min and the supernatant was transferred to a fresh tube. RNA was isolated using phenol-chloroform extraction method, followed by DNase treatment. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4289841