title: 16S rRNA sequencing of mice gut  microbiome
design: Feces of  mice were collected on days 27 post-tumor inoculation and genomic DNA was  extracted from the fecal sample using the QIAamp DNA Stool Mini Kit according  to the manufacturers instructions. The V3V4 hypervariable region of 16S  rRNA was amplified with universal primers: 338F and 806R by PCR