title: GSM4138983: 50-ver-35day-2nd; Brassica rapa subsp. pekinensis; RNA-Seq
library_construction_protocol: Total RNA was prepared from Chinese cabbage leaf tissue using Trizol (TaKaRa, Tyoko, Japan). mRNA collected from leaf tissues of the two inbred lines was fragmented and used as a template for synthesis of first-strand cDNA using random hexamers and reverse transcriptase. Second-strand cDNA was synthesized using DNA polymerase I (New England BioLabs, Ipswich, MA, USA) amd RNase H (Invitrogen, Carlsbad, CA, USA). The resultant cDNA fragments were purified, end-repaired, polyA-tailed, and ligated to index adapters following the Illumina protocol.
attributes.tag: GEO Accession
attributes.value: GSM4138983