title: Transcriptome-wide study of genetic basis for local adaptation in nine-spined sticklebacks
design: Total RNA was extracted from liver tissues using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. cDNA library was constructed with the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen, cat. no. 11917-010), according to the manufacturer's protocol. Equimolar amounts of the total RNA from each of the one male and one female were used for the marine liver library.