title: GSM2429058: Sample_Th17_H3K4me1_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Whole cell lysates were sonicated and protein-DNA complexes were isolated with antibodies. ChIP-DNA was end repaired with T4 DNA polymerase and polynucleotide kinase.  An A-base was added to the end-repaired DNA fragments. Solexa adaptors were ligated to the ChIP DNA fragments and 200-300bp size fractions were excised from 2% agarose gel stained with GelStar.  Adaptor-modified fragments were enriched by 16 cycles of PCR amplification.  The DNA library prep was validated in Bioanalyzer for quantity and size.
attributes.tag: GEO Accession
attributes.value: GSM2429058