title: GSM2425561: AN5; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen) from different cell lines. Libraries were constructed with NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs), and PCR products were purified by AMPure XP beads (Beckman). RNA library was quantified by Qubit 1.0((Invitrogen) and analyzed with agilent 2100 bioanalyzer (Agilent) for size distribution, sequenced with Illumina Hiseq-2500 in single mode (1x50nt). libraries were prepared for sequencing using NEBNext Ultra DNA Library Preparation Kit for Illumina
attributes.tag: GEO Accession
attributes.value: GSM2425561