title: Illumina HiSeq 1500 sequencing; ATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.
design: ATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.
library_construction_protocol: Cells were cultivated and harvested as described in Schaeffner et al. 2019, (https://www.biorxiv.org/content/10.1101/317354v1). Modification of nuclear DNA was done after permeabilization and transposase processing as described in detail (https://www.biorxiv.org/content/10.1101/317354v1). Cells were either left untreated or were induced with Doxycycline of a final concentration of 100 ng/ml for 15 h. DNAs were isolated as described in Schaeffner et al. 2019 (https://www.biorxiv.org/content/10.1101/317354v1). Libraries were constructed as described in Schaeffner et al. 2019 (https://www.biorxiv.org/content/10.1101/317354v1).
attributes.tag: Experimental Factor: stimulus
attributes.value: doxycycline (100 ng/ml)
attributes.tag: Experimental Factor: genotype
attributes.value: activation-domain-truncated BZLF1