title: 16S rRNA gene amplicon analysis
design: FastDNA Spin kit for soil (MPBio), V3V4 hypervariable region of the 16S rRNA gene amplified with 341f and 785r, then following protocol https://support.illumina.com/documents/documentation/584 chemistry_documentation/16s/16s585metagenomic-library-prep-guide-15044223-b.pdf.