title: GSM3439964: BPA rep2 liver RNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: A total of 18 RNA samples were isolated from gonadal adipose, hypothalamus and liver tissues from male offspring using the AllPrep DNA/RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Samples were processed for library preparation using TruSeq RNA Library Preparation Kit (Illumina, San Diego, CA) for poly-A selection, fragmentation, and reverse transcription using random hexamer-primers to generate first-strand cDNA. Second-strand cDNA was generated using RNase H and DNA polymerases, and sequencing adapters were ligated using the Illumina Paired-End sample prep kit. Library products of 250-400bp fragments were isolated, amplified, and sequenced with Illumina Hiseq2500 System.
attributes.tag: GEO Accession
attributes.value: GSM3439964