title: Z-3-HAC_rep3
design: (Z)-3-hexeny-1-yl acetate (abbreviated as Z-3-HAC) (98%) used in this experiment was purchased from Sigma-Aldrich, Inc. (USA). All seedlings were randomly divided into two batches. A half batch of the seedlings was first sprayed with 200 M Z11-HAC (Z-AC was first dissolved in 500 L of 95% (v/v) ethanol) twice with a 3-day interval at the rate of 10 mL per plant and the other half batch was sprayed with distilled water (with the equivalent amount of ethanol). Seven days after pretreatment, half of the control or Z-3-HAC pre-treated seedlings were exposed to salt stress treatments. Each pot was treated with 200 mL of 300 mM NaCl solution three times over a 2-day interval to a final NaCl concentration of 0.35% (w/w) in the soil, and the rest of the seedlings were treated with distilled water. A relatively moderate concentration of Z-3-HAC at 200 M and a salt concentration of 0.35% (w/w) were selected based on our previous data (Tian et al., 2019). Transcriptome and physiological parameters were determined at 7 days after the onset of NaCl treatment. Thus, four treatments were applied: control (water + water), Z-3-HAC (Z-3-HAC + water), NaCl (water + NaCl), and Z-3-HAC + NaCl (Z-3-HAC + NaCl). Throughout the experiment, all the measurements were performed randomly on the third fully expanded leaves for each treatment. At least 12 seedlings were selected for each treatment with three independent biological replicates each.ch.