title: 16S rDNA pyrosequencing of freshwater metagenome from USA:Lake Munson, Tallahassee, FL: Sample 1LM
design: Preparation for pyrosequencing entailed amplifying the 16S rRNA genes in the sediment and water samples with the universal forward and reverse primers 27 (5'-AGA GTT TGA TCC TGG CTC AG-3')  and 1492 (5'-GGC TAC CTT GTT ACG ACT T-3'), respectively.