title: GSM3439258: 4C_Seq_Forelimb_Rep3_N1; Mus musculus; OTHER
library_construction_protocol: Chromosome conformation capture (3C) template was prepared from pooled E14.5 liver, forelimb and hindlimb buds (n = 5–6 C57BL/6NJ embryos per replicate), with improvements to the primer extension and library amplification steps following Sexton et al., 2012 (doi: 10.1038/nprot.2012.071).  The template was amplified with Q5 High-Fidelity Polymerase (New England Biolabs GmbH, Frankfurt am Main, Germany) using a 4C adapter-specific primer and a pool of 6 Nkx3-2 enhancer viewpoint primers. Amplified fragments were prepared for Illumina sequencing by ligation of TruSeq adapters, followed by PCR enrichment.
attributes.tag: GEO Accession
attributes.value: GSM3439258