title: GSM4683680: Aorta Intima Resident Macrophage rep 1; Mus musculus; RNA-Seq
library_construction_protocol: The cDNA libraries were prepared from cell lysates in 10X Single-Cell Lysis Buffer (Takara-Clontech) containing 5% RNase inhibitor. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per the manufacturer's protocol. cDNA was fragmented using a Covaris E220 sonicator with the following parameters: duty cycle, 10; intensity, 5; cycles/burst, 200; and time, 180 seconds. The cDNAs were blunt ended, supplemented with an A base at the 3' end, and then ligated with Illumina sequencing adapters. The ligated fragments were amplified for 12 cycles using primers that incorporated unique index tags. The amplified fragments were sequenced on an Illumina HiSeq-3000 using single reads extending 50 bases.
attributes.tag: GEO Accession
attributes.value: GSM4683680