title: RNA-seq of immortalized human primary microglia after TNFa treatment
design: Total cellular RNA sequencing of TNF-alpha treated immortalized human primary microglia. For RNA-seq analysis, C20 cells were untreated or treated with TNF-α (10 pg/mL) for 16 h prior to isolation of total RNA, which was used for global RNA sequencing after preparing an RNA library using the Illumina® TruSeq Stranded Total RNA kit with the Ribo-ZeroTM Gold rRNA Removal kit to deplete both cytoplasmic and mitochondrial ribosomal RNA. Sequencing was performed on an Illumina HiSeq 2500 instrument at a depth of ∼35 million or more paired-end, 100 bp long, strand-specific reads per sample.