title: 454 Venom gland Transcriptome Speleonectes
design: Trizol-GTI-LiCl RNA extraction, cDNA synthesis and amplification Mint Kit (Evrogen), Size selection on Agarose gel. Fragment purification (MinElute Gel extraction kit, Qiagen),  Ligation pDNR-lib vector (Clontech) using Fast Ligation Kit (NEB), cDNA insert purification (MinElute gel extraction kit), Ligation, library generation follwoing Roche protocol - inserts nebuliyed for randomisation