title: GSM1143102: [RNA-Seq] Young yeast RNA replicate1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA isolation was performed using the MasterPure Yeast RNA Isolation Kit from Epicentre Biotechnologies (catalog no. MPY03100) following the manufacturer’s instructions. We included the DNase I digestion steps in our RNA isolation to ensure a purer sample for subsequent analyses. The quality of the RNA was confirmed by Agilent 2100 Bioanalyzer electropherograms. The total RNA was spiked-in with the external RNA Controls Consortium (ERCC) spike-in control mixes (Ambion, 4456740) based on the same number of cells for each sample. The ERCC RNA Spike-In Control Mixes used here comprise a set of 92 polyadenylated transcripts that mimic natural eukaryotic mRNAs. The RNAs range in size from 250–2,000 nucleotides in length and span an approximately 106-fold concentration range. Ribosomal RNA was removed using the ribo-zero rRNA removal kit (Epicentre, MRZH11124). cDNA libraries were prepared using the ScriptSeqTM v2 RNA-seq library preparation kit (Epicentre, SSV21106)
attributes.tag: GEO Accession
attributes.value: GSM1143102