title: GSM4141364: CRISPRi library DNA; synthetic construct; OTHER
library_construction_protocol: Genomic DNA was extracted with standard laboratory tecniques. Libraries were prepared according previous methods (e.g. Klann et al. Nature Biotechnology 2017). Briefly, the gRNAs were PCR amplified from 5 ug of genomic DNA using custom oligonucleotides custructed with sequencing adapters, custom indices, and a sequence specific gRNA construct. Libraries were purified using PCR Clean Dx beads (Aline Biosciences) before sequencing on an Illumina MiSeq.
attributes.tag: GEO Accession
attributes.value: GSM4141364