title: temporal dynamics of the phycosphere
design: DNA was amplified  using the Phire Hot Start II DNA Polymerasea touchdown round (without TAG) was preconized: 98C for 3 min; 24 cycles of 98C for 30 s, 65 to 55C for 30 s with -0.4C/cycle, 72C for 60 s; followed by 12 cycles at 55C and a final extension of 10 min at 72C. A second round PCR was used to insert the TAG-primers within the sequences at an annealing temperature of 55C. The triplicate PCR products were pooled and purified