title: Livers sample from Gallus gallus
design: Eighteen 40-day-old chicks (FAdV4 antigen and antibody negative) were randomly divided into the control group or infection group. Chicks in the infection group were administered an intramuscular injection of 0.5 mL FAdV4 (TCID50 = 10-6.23/0.1 mL), and those in the control group were injected with the same volume of sterile saline.At 2 days post-infection with FAdV4, the livers in the two groups were collected and total RNA was extracted using an RNA extraction kit (Saffer, China). The mRNA was separated with magnetic beads to generate a sequence library using the Illumina HiSeq 2000 (Illumina, Shenzhen, China).