title: GSM4138574: RNA-seq, KP clone 1-2 expressing mutant T6B-YFP; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were extracted from cells using TRIzol Reagent following the manufacturer's instructions , treated with DNase and precipitated with isopropanol. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 1µg of total RNA with an RNA integrity number varying from 9-10 underwent ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4138574