title: GSM2425735: RNA_97L Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Methylation: Genomic DNA from tissues and cell lines was extracted using QIAamp DNA Mini Kit (Qiagen, 51306, Valencia, CA). 3μg genomic DNA was fragmented into 300-500bp by sonication. ChIP:Approximate 300 ug fixed chromatin and 5 ug antibody were used for each ChIP pull-down. RNA-seq: We extracted approximate 6 micrograms of total RNA, removed ribosomal RNA (rRNA) according to the manufacturer's instructions with minor modifications. Libraries were prepared for sequencing using standard Illumina protocols DNA Methylation sequencing, ChIP-seq and RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM2425735