title: GSM1973865: glucose wt  9a; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Log phase cells were harvested and frozen in liquid Nitrogen. Total RNA was extracted using a NucleoSpin® 96 RNA kit (Macherey-Nagel), substituting β-mercaptoethanol with DTT RNA extracts were fragmented and polyadenylation was selected using PolydT beads. cDNA was prepared using Smartscribe and UMI-containing barcoded primers, and subjected to RNnase H treatment. A second adaptor was subsequently ligated.
attributes.tag: GEO Accession
attributes.value: GSM1973865