title: Positive control equimolar DNA extracts of insect from diet items of Antrozous pallidus: insects
design: Positive control, DNA extracts from insect samples used in controlled feeding were pooled in equimolar amounts following extraction. The sample was subjected to a two step PCR protocol; first universal primers were used to individually amplify markers before preforming a barcoding PCR. After which samples subject to bead clean up and pooled in equimolar concentration. Sequencing was conducted on an Illumina Miseq.