title: ChIP-Seq of H4K16ac: adult female, HL60 cell line treated with DMSO + ATRA
design: For high-throughput sequencing of H4K16ac-enriched genomic regions (ChIP-seq), CD3+ T cells and neutrophils isolated from human peripheral blood were freshly processed using the SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling, 9003). The amount of Microccocal Nuclease required to digest DNA to the optimal length was determined as 2.5 l per 2 x 107 cells. Library preparation and sequencing were performed at Centre de Regulaci Genmica (CRG) in Barcelona on an Illumina HiSeq 2000 system in 50-single end mode.