title: Arsenate reductase gene from sediments and groundwater samples: Groundwater Well       10
design: Amplicon sequencing of arsenate reductase gene (arrA) from sediment and         groundwater samples. Arsenate reductase gene (arrA) was amplified from both sediment and groundwater samples collected form Cache Valley area. PCR amplicon libraries for arrA gene was amplified with new developed arrA specific primers, containing sequencing adopters and 8-base barcodes. PCR amplification was carried out by FastStart high-fidelity Psystem enzyme (Roche Applied Sciences). Amplified arrA genes were purified with Agencourt AMPure beads (Beckman Coulter, Brea, CA). Purified, adapter, and unique barcode attached PCR products from each of the nine DNA extracts were pooled and sequenced through Personal Genome Machine (PGM™) System.