title: genome sequencing of early passage Leishmania donovani
design: High quality genomic DNA was extracted from stationary phase promastigotes  early(HTI4) passage of L. donovani AG83 using a commercial procedure as recommended by the manufacturer (Qiagen, Germany). Size check, integrity and presence of contaminants in the DNA samples were assessed through gel electrophoresis. DNA purity was measured using a Nano Drop 2000 spectrophotometer (Thermo Scientific, MA, USA). De novo paired end sequencing was done on Illumina HiSeq 2500 .