design: An aliquot of the DNA preparation served as template DNA for                 sequencing on the GS FLX Titanium platform. The sequencing library was constructed                 according to the protocol of the GS Titanium General Library Prep Kit (Roche Applied                 Science). After titration of the library using the GS Titanium SV emPCR Kit (Roche                 Applied Science), a full sequencing run was carried out on the GS FLX Titanium                 platform.