title: RNAseq of homo sapiens: SH-4-54 resistant MDA-MB-231 cells 3
design: RNA quality was validated using the RNA 6000 Nano kit and a 2100 Bioanalyzer (Agilent). A RNA library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina, with enrichment of poly-A mRNA achieved using the Next Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Each library was sequenced on the Illumina HiSeq 1500 platform (Illumina) using the HiSeq Rapid V2 chemistry with onboard cluster generation and 70 bp single-end reads. Each biological replicate was split between two lanes to mitigate lane effects.