title: GSM3702497: AB4835; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized and popliteal and inguinal LNs were dissected. Cells were collected, filtered through a 70µm cell strainer, washed and maintained strictly at 4°C. Dendritic cells were enriched by negative selection. Cells were stained with FACS antibodies, then sorted into 384-well plates Single-cell RNA-seq libraries were prepared as previously described {Jaitin, 2014}. In brief, mRNA from single cells sorted into capture plates were barcoded and converted into cDNA and then pooled using an automated pipeline. The pooled sample was linearly amplified by T7 in vitro transcription, and the resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with pool barcodes and Illumina sequences during ligation, RT, and PCR. Each pool of cells was tested for library quality and concentration as described previously {Jaitin, 2014} single cell RNA-seq for gene expression quantitation; Note: Second read contained only cell and molecule barcodes. This information was appended to the fastq entry header
attributes.tag: GEO Accession
attributes.value: GSM3702497