title: GSM2424731: ChIP input; Homo sapiens; ChIP-Seq
library_construction_protocol: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, 69506) according to the manufacturer’s instructions. For ChIP, transfected cells were subjected to chromatin immunoprecipitation (ChIP) with a commercially available kit ChIP-IT Express Enzymatic (53009-AF, ActivMotif, distributed by Nordic Biolabs) and an anti-HA tag antibody (C29F4, Cell Signaling) according to the manufacturer's instructions. Library Preparation was followed by DNA-end repair, 3'-dA overhang and ligation of sequencing adaptors, PCR amplification and size selection (100-300bp, including adaptor sequence).
attributes.tag: GEO Accession
attributes.value: GSM2424731