title: amplicon of HIV pol region : human blood cellular DNA
design: The HIV-1 pol region was amplified using an in-house-designed PCR system from plasma-derived viral cDNA and proviral DNA using specific primers (MAW 26: 5-TTGGAAATGTGGAAAGGAAGGAC, RT21: 5-CTGTATTTCTGCTATTAAGTCTTTTGATGGG). Specific primers (F1: 5-GCCAGGAATGGATGGCCCAAAAG-3, R1: 5-TGGTGATCCTTTCCATCCCTGTGG-3; F2: 5-AGTATACTGCATTTACCATACC-3, R2: 5-CTTCTGTATGTCATTGACAGTCC-3) were used to amplify two partially overlapping amplicons which together spanned the HIV-1 pol region, including the reverse transcriptase region codons 14-258. The products of the PCR were used as the template for constructing the deep sequencing libraries. Barcodes were introduced using specific primers to distinguish different samples.