title: Inoculum from Groesbeek activated sludge in  June 2019 replicate 1
design: 16S rRNA gene sequencing was  performed on an Illumina Miseq. The primers used for previous PCR  amplification were Bac341F (5CCTACGGGNGGCWGCAG3) and Bac785R (5  GACTACHVGGGTATCTAATCC 3) (Klindworth et al., 2013).