title: Cucumber Gy14 fruit peel 8 days post pollination RNAseq-REP2
design: Peels were collected from 8 and 16 dpp of Poinsett 76 and Gy 14 using a vegetable peeler and immediately frozen in liquid nitrogen.  Three fruit (biological replicates) were collected for each age and genotype. Samples were ground using a mortar and pestle in liquid nitrogen.  RNA extraction was performed using the MagMAX Plant RNA Isolation Kit protocol (Thermo Scientific, Waltham, MA) with slight modifications: 100-150 mg of ground tissue were added to 1000 L of lysis buffer. High-throughput RNA extraction was performed in 96-well format, on a KingFisher Flex Purification System.  Immediately after the run was complete, the 96-well plate was transferred to storage at -80C.  RNA concentration and quality were measured using Qubit 2.0 Fluorometer (Life Technologies, Inc.) and LabChip GX (Perkin Elmer) respectively.