title: GSM2425613: D18-3_090; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNAs were prepared from the leaves of anac017, anac082 and anac090 mutants and WT (n=3 for each). The RNA integrity of the samples were confirmed (RNA integrity number ≥7.8) by using the Agilent Technologies 2100 BioAnalyzer. According to the manufacturer’s instructions, Poly(A) mRNA was isolated from the RNA (2 µg) and fragmented by using Illumina Truseq™ Stranded mRNA LT Sample Prep Kit with poly-T oligo-attached magnetic beads. After the reverse transcription of the RNA fragments by using Superscript II reverse transcriptase (Life Technologies, USA), the strand-specific cDNA libraries were constructed with adaptor-ligation and sequenced by Illumina HiSeq 2500 system.
attributes.tag: GEO Accession
attributes.value: GSM2425613