title: GSM4290094: t9_BC rep1; Fragaria x ananassa; RNA-Seq
library_construction_protocol: RNA was extracted from the plant leaves according to Luypaert et al. (2017) and described in detail in Debode et al. (2018). DNase treatment was done using the DNA-free kit (Ambion, Thermo-Fisher Scientific). For cDNA synthesis, the Tetro cDNA Synthesis kit (Bioline) was used, starting from 1.5 µg of DNA-free RNA. Samples were first enriched in mRNA by targeting the 3' polyA-tail using poly-T oligos attached to magnetic beads. The enriched mRNA was converted into cDNA . Samples are multiplexed by using unique barcodes (Kukurba & Montgomery, 2015) and pooled. Sequencing of the library was done using the Illumina HiSeq 3000 platform .
attributes.tag: GEO Accession
attributes.value: GSM4290094