title: Illumina HiSeq 2000 paired end sequencing; Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)
design: Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)
library_construction_protocol: The C5 hiPSC was reprogrammed from the somatic cells derived from post-mortem human fetal neural tissue which was acquired at the time of surgical termination of pregnancy in the day surgery unit of Addenbrookes Hospital, Cambridge. Full informed consent was obtained from participating mothers. All generated lines are unlinked anonymous.. All ​related procedures and experiments were approved by the hospital and Sanger institute. M10 cells were derived from pig fetus fibroblast by reprogramming. Those pig fetus fibroblast cells were derived from a 28-day normal healthy male pig fetus skin. K3 cells were derived from 5-day preimplantation embryos, again, healthy male. All the related embryos and fetus experiments were approved by the Niedersaechsisches Landesamt fuer Verbraucherschutz und Lebensmittelsicherheit, LAVES, Oldenburg Germany. Abbreviations used: PFF means the pig fetus fibroblast. Emb means the origin is 5-day embryos. Parth means parthenogenetic embryos, PGCLC means the primordial germ cell-like cells differentiated from K3 cells.  Total RNA was purified by RNeasy Minikit (Qiagen) according to the manufacturer's manual. RNA concentration was determined by Nanodrop, and 500 ng of total RNA was used for library construction using KAPA Stranded mRNA-Seq Kits. Sequencing was performed on Illumina HiSeq 2000 machines with 75 bp pair-end mode. KAPA Stranded mRNA-Seq Kits
attributes.tag: Experimental Factor: time
attributes.value: not applicable
attributes.tag: Experimental Factor: cell line
attributes.value: K3
attributes.tag: Experimental Factor: organism
attributes.value: Sus scrofa