title: WGS of L. monocytogenes isolate CFIAFB20190082
design: Brain Heart Infusion broth was inoculated  with a single colony of E. coli and incubated for 4 to 6 h at 35C. Genomic  DNA was isolated from 400 L of culture using the Maxwell 16 Cell SEV DNA  Purification kit (Promega, Madison, WI). DNA was then quantified using the  Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington,  ON). Sequencing libraries were constructed from 1 ng of gDNA using Nextera XT  DNA Sample Preparation and Index Kits (Illumina, Inc., San Diego, CA)  according to manufacturers instructions. Genomic sequencing was performed on  the Illumina MiSeq Platform (Illumina, Inc.) using a 600-cycle MiSeq Reagent  kit v3 (Illumina, Inc.).