title: 16S rRNA Sequencing of Centropomus undecimalis microbiome: whole larvae
design: We investigated the impacts of age and probiotic treatment on the common snook rinsed larvae microbiome during a high mortality event. We amplified the bacterial 16S rRNA gene using universal primers 515F/806R (Caporaso et al. 2011) and sequenced them using Ion Torrent PGM. Sequences have been processed (depleted of barcodes and primers, removal of sequences < 150 bp, removal of sequences with > 8 bp homopolymer runs, denoised). OTUs were defined at 97% sequence similarity and taxonomically classified using a curated database (derived from GreenGenes, RDPII, and NCBI) and chimeric sequences as well as those identified as non-bacterial have been removed.