title: GSM2425583: dCas9-BFP-DNMT3B (500 ng); Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, 69506) according to the manufacturer’s instructions, and subject to WGBS SOP analysis in BGI tech. For Bisulfile-Seq, genomic DNA was fragmented by sonication to a mean size of 250bp using a Bioruptor (Diagenode, Belgium). Bisulphite conversion was conducted with the EZ DNA Methylation-Gold kit (ZYMO). The libraries were sequenced on Illumina HiSeq X Ten. The 48502 bp lambda DNA was used as an extra reference for calculating the bisulphite conversion rate. Nearly complete (>99%) bisulfite conversion was documented in all libraries.
attributes.tag: GEO Accession
attributes.value: GSM2425583