title: GSM4634776: N-term-mCherry-Pdr802 IP rep 3; Cryptococcus neoformans; ChIP-Seq
library_construction_protocol: The remaining material was incubated with rabbit IgG anti-mCherry antibody (Abcam, ab213511) tethered to protein A sepharose ('IP') or sepharose alone ('Mock') overnight at 4°C. The beads were then washed, incubated at 65°C to reverse DNA-DNA and DNA-protein crosslinks and the DNA recovered by phenol/chloroform/isoamyl alcohol (25:24:1) extraction, ethanol precipitation, and resuspension in nuclease-free water. The samples were sonicated to obtain fragments of about 200bp. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina E7645L, following the manufactrer's instructions
attributes.tag: GEO Accession
attributes.value: GSM4634776