title: Illumina for the genome of Bassia scoparia: Mature Leaf Tissue
design: The PCR-free shotgun library was constructed with the Library Construction kit from Kapa Biosystems. The mate-pair libraries were constructed with the Nextera Mate Pair library Sample Prep kit (Illumina, CA), followed by the TruSeq DNA Sample Prep kit. The libraries were quantitated by qPCR, pooled  and sequenced on one lane (mate-pairs) and 3 lanes (shotgun) for 151 cycles from each end of the fragments on a HiSeq2500 using a TruSeqRapid SBS sequencing chemistry version 13. The fastq files were generated and demultiplexed with Casava1.8.2