title: GSM2428210: Endoderm_Donor_Kdm5wt_1 (Group4); Xenopus laevis; RNA-Seq
library_construction_protocol: RNA extraction and preparation of cDNA library for sequencing: Embryonic tissues (endoderm and ectoderm, as indicated) were dissected from embryos at the respective stage and frozen at -80°C. One tissue from one embryo was processed individually. RNA extractions were performed using Qiagen RNeasy Mini kit (Qiagen, 74106) according to the manufacturer's protocol including the DNase step. RNA was eluted in 40ul of DEPC H2O and 500 ng RNA was used to generate a cDNA sequencing library with Illumina TruSeq kit (RS-122-2001), according to the manufacturer's protocol. Library construction: Illumina TruSeq RNA sample prep kit for RNA-seq.
attributes.tag: GEO Accession
attributes.value: GSM2428210