title: RNA-seq  of Solenopsis invicta: SB/SB virgin queens, pair 1
design: Queen pupae were isolated in subcolonies, 18 days after eclosure the queens were dealated, and frozend after subsequent 7 days. All the virgin queens were observed laying eggs. Trizol fixed samples were use use to extract RNA using strandard Trizol Reagent protocol. RNA were then purified using Direct-zol RNA MiniPrep (Zymo Research). cDNA libraries were prepared for sequencing using standard Illumina protocols.