title: GSM3439086: MEF2_Control ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIP-seq, hearts were excised quickly, rinsed in PBS and snap frozen in liquid nitrogen. ChIP-seq experiments were performed by Active Motif Inc. The samples were crosslinked with PFA, homogenized and sonicated to fragment DNA. The DNA/protein complexes were pulled down by antibodies against  HA tag (Abcam, Catalog #ab9110), TBP (Abcam, Catalog #ab51841) or MEF2C (Santa Cruz, Catalog #sc-313). The crosslinking was reversed and DNA was purified and subjected to library construction. For RNA-seq, the left ventricle tissues were quickly harvested and washed in cold PBS and homogenized in trizol. Trizol manual was followed to extract RNA. Total RNA was treated with DNASE-I (New England Biolabs, Catalog #M0303S) to eliminate genomic DNA and then cleaned using RNA cleanup Kit (Qiagen, Catalog #74204). For RNA-seq, Illumina TruSeq Stranded mRNA Library Prep Kit (catalog # 20020595) was used to prepare the sequencing library. For ChIP-seq, the sequencing library was prepared by Active Motif, Inc.
attributes.tag: GEO Accession
attributes.value: GSM3439086