title: Transcriptional Activation of human mir-498(46) cistron
design: the RNA input for each sample was ligated to a 3 adaptor barcoded sequence, pooled, size selected, and gel purified, followed by 5 adapter ligation and then subjected to size selection and gel purification. The cDNA library preparation was completed by second strand synthesis using SuperScript III, alkaline RNA hydrolysis, and PCR amplification for 10 cycles. mRNA libraries were prepared by utilizing the Illumina TruSeq Stranded mRNA LT protocol using 500 ng total RNA and NEBs Protoscript II reverse transcriptase for the first-strand cDNA synthesis according to the manufacturers protocol.