title: Nasonia vitripennis HVRx outbred laboratory population pooled genome sequence PairedEnd
design: To resequence the HVRx base population, DNA from 20 pooled females from generation 4  was extracted and purified using Qiagen DNeasy columns (Qiagen, Hilden, Germany). A 101 bp unpaired and a 76 bp paired-end library were constructed and subsequently sequenced by the University Medical Centre Groningen (UMCG) Genome Analysis Facility according to standard Illumina protocols. The unpaired library was run on a single lane and the paired-end library on five lanes of an Illumina Genome Analyzer II.  Reads were aligned to the v2.0 of the Nasonia vitripennis genome (Werren et al., 2010) using Mosaik Aligner v1.1.0021 (http://bioinformatics.bc.edu/marthlab/Mosaik).