title: Bacterial community composition of dog stool sample based on 16S rRNA gene amplicons
design: A part of the 16S rRNA gene was amplified with fusion primers containing Bacteria-specific sequences Bakt_341F (5'-CCT ACG GGN GGC WGC AG-3', Klindworth et al. 2013) and modified Bakt_805R (5'-GAC TAC NVG GGT ATC TAA TCC-3', Herlemann et al. 2011), with Fluidigm CS1 and CS2 universal sequences at their 5' ends.