title: PacBio  DNA-seq of Bacteroides xylanisolvens strain Funn3
design: DNA  extraction with Qiagen Genomic Tip g/500. Library was prepared using Pacific  Biosciences protocol for SMRTbell Libraries using PacBio Barcoded Adapters  for Multiplex SMRT Sequencing. DNA was sheared to 10 kb fragments using  g-tubes from Covaris. Samples were pooled during library prep using equal  mass, eight samples in each library. Final library was size selected using  0.45x Ampure PB beads. The library was sequenced on Pacific Biosciences  Sequel instrument using Sequel Polymerase v2.0 and Sequencing chemistry  v2.10. Loading was performed by diffusion, one SMRT cell was sequenced. Movie  time: 600 min, pre-extension time: 120 min. Reads were demultiplexed using  Barcoding pipeline on SMRT Link (5.1.0.26412, SMRT Link Analysis Services and  GUI 5.1.0.26411) using default settings with minimum barcode score 40.