title: 16S rRNA amplicons from cold-water coral microbiome of Primnoa pacifica
design: The source of this coral microbiome is a sample of Primnoa pacifica collected by SCUBA from the Gulf of Alaska in 2011.  The coral tissue was preserved using RNAlater overnight at 4űC before extended storage at -20űC.  DNA was extracted according to the protocol of Sunagawa et al. (2010) and sequenced with primers targeting the V4-V5 variable region of the 16S rRNA gene using 454 pyrosequencing with GS FLX Titanium chemistry.