title: RNA-Seq of homo sapiens: adult female plasma
design: cfRNA was extracted from 0.75 to 3 mL of plasma using Plasma/Serum Circulating RNA and Exosomal Purification kit (Norgen, cat 42800). The residue of DNA was digested using Baseline-ZERO DNase (Epicentre) and then cleaned up using RNA Clean and Concentrator-5 kit (Zymo). RNA was eluted in 12   elution buffer. cfRNA sequencing libraries were prepared by SMARTer Stranded Total RNAseq - Pico Input Mammalian kit (Clontech) from 6   of eluted cfRNA according to the manufacturer  instructions. Short read sequencing were performed on Illumina NextSeq 500  (2x75 bp) platform to the depth of more than 10 million reads per samples.