title: GSM2425575: drb7.2 rep1; Arabidopsis thaliana; ncRNA-Seq
library_construction_protocol: Total RNA from two independent biological replicate of Col-0 and drb7.2 flowers was extracted with Tri-Reagent (Sigma) according to manufacturer’s instructions. A 6μg weight of total RNA was used for preparation of small-RNA libraries RNA libraries were prepared for sequencing using standard Illumina protocols. Briefly, a first acrylamide gel purification of small RNA between 18-30 was performed. The libraries were then constructed using the kit TruSeq Small RNA preparation kit from illumina following manufacturer's instructions and the libraries were sequenced using a Illumina HiSeq 2500 (Fasteris, Switzerland)
attributes.tag: GEO Accession
attributes.value: GSM2425575