title: GSM4141359: GSC23 EGFR enhancer 1 4C-seq; Homo sapiens; OTHER
library_construction_protocol: DNA was extracted with standard laboratory tecniques. Libraries were prepared according previous methods (Splinter et al. Methods 2012). Briefly, cells were crosslinked with formaldehyde and lysed to extract nuclei then digested with DpnII and ligated with T4 DNA ligase. Next, crosslinks were reversed with proteinase K at 65C. After, the DNA was digested with CviQI and ligation. Libraries were prepared from the circular DNA with PCR amplification with custom oligos detailed in Splinter et al. and designed for the particular 4C anchoring point. Libraries were cleaned with PCR Clean Dx beads before sequencing on a Illumina HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM4141359