title: Syngnathus scovelli non-pregnant male brood pouch
design: Name- S. scovelli non-pregnant male brood pouch cDNA pool  Strategy- Shotgun cDNA pyrosequencing  Source- Pooled total RNA (5 individuals) from male pipefish brood pouch tissue  Selection- Qiagen oligotex mRNA enrichment  Layout- Single-end 454 GS FLX Titanium  Library Construction Protocol- cDNA generated from polyA  RNA using modified Clontech Smart cDNA library construction kit (random hexamer priming, 17 cycles of LD PCR)