title: Propionibacterium acidipropionici F3E8 Cell 1
design: DNA-sequencing and de novo assembly of the different Propionibacterium strains, first DNA wasereas extracted using PureLink Ggenomic DNA Mmini kit (Invitrogen Cat. No. K1820-01) and quantified using Nanodrop 1000 (Thermo Scientific) and Qubit dsDNA BR assay kit (Life Technologies Cat. No. Q32850).  The quality of the DNA was determined by running a 1% agarose gel with DNA gel stain SYBR safe (Life Technologies Cat. No. S33102).  The gel was visualized in using a ChemiDoc MP system (Bio-Rad). The PacBio library preparation was performed using the protocol for 20 Kb selected with the BluePippin system. The sequencing chemistry was the most recent released P6-C4 by PacBio and loaded by magnetic beads.  The genome assembly was performed with the SMRT Pportal.