title: GSM4291943: JN13 - A24 - CI21; Rattus norvegicus; RNA-Seq
library_construction_protocol: Animals were anesthetized with isoflurane, decapitated, and the brain was removed and flash-frozen in methylbutane before storage in a -70°C freezer. Brains were dissected using a brain matrix. Based on anatomical landmarks on the ventral surface of the brain, sections 2 mm thick containing the striatum were taken with guidance from a rat brain atlas. A 2.0 mm diameter punch (Harris Unicore™) of the NAcsh was then taken. RNA was isolated using standard Trizol® (Invitrogen, #15596026) extraction. RNA was prepared using the Nugen Ovation RNA-seq system. cDNA was quantified using Nanodrop and sheared to approximately 300 base pair fragments. Libraries were generated using the Kapa Biosystem library preparation kit. Fragments were then end repaired and A-tailed. Sequencing was performed on a 1x75 flow cell using an Illumina NextSeq500 instrument.
attributes.tag: GEO Accession
attributes.value: GSM4291943