title: GSM3439401: Hand2as+/+_E11.5_scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: E11.5 hearts of wild type and Hand2asF/F from heterozygotes intercrosses were subjected for Single-cell RNA-seq. We harvested six embryonic hearts for each genotype. Embryonic hearts were trypsinized (0.25%, 5min, 37℃) individually, and subjected for Fluorescence-activated cell sorting (FACS) after 7-Aminoactinomycin D (AAT Bioquest, 17501) staining for collection of living single cells. Single cells from six embryonic hearts were combined as a single sample for 10X Genomics Single Cell 3' library construction (10X Genomics, PN-120237). The RNA libraries were constructed by following the manufacture recommended procedure to get ~5,000 cells barcoded per sample.
attributes.tag: GEO Accession
attributes.value: GSM3439401