title: Cucumber  Vlaspik DNAseq
design: Tissue samples (50100 mg fresh weight) were collectedfrom young (not fully expanded) leaves, freeze-dried, and ground to a fine powder using 5/32 stainless steel balls (AbbottBall, West Hartford, CT) in a Retsch Mixer Mill (Retsch, Newtown, PA). DNA was isolated using the Omega Mag-Bind Plant DNA DS Kit (M1130, Omega Bio-Tek, Norcross, GA) on a Kingfisher Flex Magnetic Particle Processor (Thermo Scientific, Waltham, MA). The kit protocol was followed except that the initial 56 C incubation was extended to 60 min instead of 30 min. The DNA was quantified using the Quant-iT PicoGreen dsDNA Kit (Invitrogen, Carlsbad, CA) in a 384-well for- mat on a CFX384 C1000 Real-Time thermal cycler (BioRad, Hercules, CA). Normalization to 30100 ng/ul was done using a GBFit Arise Pipetting System (Pacgen Inc., Irvine, CA).