title: GSM4011044: rancRNA_18_total mRNA_rep2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Yeast spheroplasts were electroporated with or without RNA, recovered and harvested by centrifugation. Pellets were opened using a FastPrep. Lysates were cleared by centrifugation and total RNA was extracted by hot acid phenol extraction. Poly-A-Enrichment via oligo-dT-beads. PolyA enriched mRNAs were transformed into a cDNA library using the Illumina TruSeq stranded mRNA Kit. Library size was determined using Qbit.
attributes.tag: GEO Accession
attributes.value: GSM4011044