title: RNA-seq of murine astrocytes: ER Stress 2
design: Libraries were built using 750 ng RNA and KAPA stranded mRNA kit as per manufacturers protocol. The libraries were then quantified with the Qubit and run on the Bioanalyzer using a High Sensitivity DNA chip to determine average size. They were then pooled at an equimolar ratio and sequenced (paired end (PE) 100bp) on the HiSeq 2500.