title: RNA-seq of Paramormyrops kingsleyae: Brain
design: tissue samples were taken from a multiple juvenile Paramormyrops kingsleyae collected in 2009 from Bambomo Creek (PKING1, PKING2, and PKING3; EOD for all specimens was P0-absent).  Tissues sampled were: brain, kidney, electric organ, skeletal muscle, the skin along the flank (unenriched for electroreceptors), the skin of the face (enriched for electroreceptors), spinal cord, gonadal tissue, gill, spleen, pectoral fin, and swim bladder.  Each tissue sample was dissected freshly into RNAlater, stored at 4ûC overnight, and then transferred to -20ûC until RNA isolation.  RNA-isolation was performed using an RNA-easy Mini Kit for lipid tissues (Qiagen).  Quality and concentration were assessed using a spectrophotometer and Qubit.  RNA integrity was assessed using a BioAnalyzer (Agilent), all RNA samples sequenced had a RIN score >8 .  Sequencing libraries were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit LT.