title: Positive control equimolar DNA extracts of pollen from diet items of Leptonycteris yerbabuenae: pollen grains
design: Positive control, DNA extracts from pollen samples used in controlled feeding were pooled in equimolar amounts following extraction. The sample was subjected to a two step PCR protocol; first universal primers were used to individually amplify markers before preforming a barcoding PCR. After which samples subject to bead clean up and pooled in equimolar concentration. Sequencing was conducted on an Illumina Miseq.