title: GSM3703269: Wildtype X. laevis at NF stage 45, Biological Replicate 1; Xenopus laevis; RNA-Seq
library_construction_protocol: RNA libraries were generated with the Qiagen RNeasy Mini kit and DNAse treated prior to generating cDNA libraries for RNAseq. RNAseq cDNA libraries were constructed with the Illumina Truseq RNA Library Preparation Kit v2, following the manufacturer's protocol. We applied Quibit analysis to quantify the RNAseq cDNA libraries, which were then pooled and run on Illumina Hi-Seq 2500 at the Tufts University Core Facility to generate 100-bp single end reads.
attributes.tag: GEO Accession
attributes.value: GSM3703269