title: RNA-seq of Homo Sapiens: skin biopsy : Sample CTS_SAMPLE_7
design: "RNA was extracted using a combination of phenol extraction and column purification. Samples were homogenized in Trizol and mixed with chloroform before spinning to induce phase separation. The aqueous liquid phase containing the nucleic acids was then removed and added to the columns of the High Pure RNA tissue kit (Roche Diagnostics, UK). RNA was purified using repeated wash steps and DNAse treatment.  The extracted RNA was sent for sequencing at the Wellcome Trust Centre for Human Genetics in Oxford. All samples were normalised to 650ng prior to library preparation using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, UK) and poly-A enrichment."