title: Shotgun sequencing of Horonobe groundwater: E-160m-2014
design: DNA was extracted from the biomass retained on 0.2 um filters using the Extrap Soil DNA Kit Plus ver. 2 (Nippon Steel & Sumikin Eco-Tech Corporation) and sent for 150 bp paired-end sequencing with a 550 bp insert size by Hokkaido System Science Co., Ltd. using an Illumina HiSeq2000.