title: Illumina HiSeq 2500 paired end sequencing
design: gDNA-Seq libraries to generate 125 bp read pairs were constructed
library_construction_protocol: One nanogram of DNA per sample was used for the construction of the libraries. First, the DNA is simultaneously fragmented and tagged with adaptater sequences by a transposome (“tagmentation step). Then, the tagmented DNA is enriched with 12 cycles of PCR using indexed PCR primers. After clean-up using Ampure XP beads (Beckman Coulter Genomics, ref.A63881), the final libraries were validated with a DNA High Sensitivity Labchip on a Bioanalyzer (Agilent) and quantified with microfluorimetry (Qubit).