title: High throughput sequencing using long-SAGE to compare poly(A) and CUT RNA fractions in yeast
design: High throughput sequencing using long-SAGE to compare poly(A) and CUT RNA fractions in yeast
library_construction_protocol: Poly(A)+-enriched RNA from the wild type strain was purified using Dynabeads Oligo (dT)25 (Dynal, Invitrogen). RNA from the CUT fraction was obtained by Cbp20 TAP purification. 3prime Long-SAGE tags were obtained by an improved 3prime Long-SAGE technique adapted from Wei et al, PNAS, 2004. The 18nt-long 3prime tags resulted from MmeI cleavage were ligated into tetratags. These tetratags correspond to 2 ditags and 3 delimiting linkers. First linkers bears a barcode sequence to distinguish the two conditions.
attributes.tag: Experimental Factor: StrainOrLine
attributes.value: BY4742
attributes.tag: Experimental Factor: Genotype
attributes.value: MAT_ ura3_0 leu2_0 his3_1 lys2_0
attributes.tag: Experimental Factor: StrainOrLine
attributes.value: LMA587
attributes.tag: Experimental Factor: Genotype
attributes.value: MAT_ ura3_0 leu2_0 his3_1 CBP20-TAP::HIS3 rrp6_::pGID2 Hygrotet07-TRF4