title: GSM4140750: SW480; Homo sapiens; OTHER
library_construction_protocol: The ribosome-bound RNAs were harvested using Trizol reagent. The ribosome-bound RNAs were used for the construction of sequencing libraries. Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA) was used to remove rRNA, and rRNA free residue was cleaned up by ethanol precipitation. Sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) according to manufacturer's instructions.In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). The purified libraries were validated by the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4140750