title: Brucella melitensis 70000565
design: The DNA samples were prepared for multiplexed (single-read, 82 cycles) sequencing using Illumina GAIIx genome analyzer (Illumina Inc., San Diego, CA). For each isolate, genomic library preps were generated using Nextera DNA Sample Prep Kit. Post library quality control and quantification was done using BioAnalyzer 2100 high-sensitivity chips and KAPA SYBR FAST Universal 2X qPCR Master Mix. Post processing of reads was performed by the RTA/SCS v1.9.35.0 and CASAVA 1.8.0.  Reads were trimmed back to the Q30 level using CLCBio's quality_trim program, CutAdapt v0.95 was used to excise adapter and transposon contamination.