title: 16S rRNA genes: fish gut  microbiome, fish 7
design: Bacterial DNA was extracted using the DNeasy Blood &Tissue Kit (QIAGEN, USA). Extracted DNA was subjected to PCR targeting the bacterial 16S rRNA gene using barcoded primers (341F (CCT ACG GGN GGC WGC AG) and 785R (GAC TAC HVG GGT ATC TAA TCC). Sequences were amplified at the Ramaciotti Centre for Genomics (UNSW Sydney, Australia).