title: TCR repertoire sequencing revealed the spatial heterogeneity of TIL in primary liver cancer: Sample P5T2_TCR
design: The total RNA was extracted from each sample, and then was applied to prepare libraries of TCR. A series of three nested PCR reactions as described previously (PMID: 24952902) were performed to obtain a TCR repertoire; the ARM-PCR procedure (PMID: 20080641) was slightly modified in this assay. After complete three nested PCR reactions, DNA with lengths of 200-500 bp was separated and purified with a QIAquick gel extraction kit (Qiagen, cat. no. 28706). Amplicons were then sequenced