title: GSM4141513: Naive CD4+ T cells replicate 2; Mus musculus; ATAC-seq
library_construction_protocol: Single cells were extracted from suspensions of spleen from 4 male and 2 female individual mice that were pooled for sorting TH1 and TFH cells or one individual female litter-mate mice for naive CD4 T cells Cells were sorted by flow cytometry (using a FACSAria III, BD Biosciences) and gated for dump-CD4+CD44+gp66+ expression and either Ly6C+ PSGL-1+ (Th1) Ly6C-PSGL-1- (TFH) or dump- CD4+ CD44- CD62L+ for naive CD4 T cells. The sorted cell suspension from each sample were prepared according to Omni ATAC protocol that was previously published In brief, DNA was isolated, tagmented with a transposase, amplified by PCR and purified
attributes.tag: GEO Accession
attributes.value: GSM4141513