title: 16S rDNA metagenome of a high-nitrate wastewater bioreactor
design: Primer sets 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and A518R (5'-ATTACCGCGGCTGCTGG-3') (Ionescu et al., 2012) were used for bacterial sequences. The V1–V3 region of the 16S rDNA gene was amplified by PCR from GA DNA using primers adapted for the Roche-454 Titanium kit.