title: ChIP-Seq analysis of H3K4me1 in human CD25int CD127+ Tmem cells
design: Single read - Illumina
attributes.tag: CHIP_PROTOCOL_BEAD_TYPE
attributes.value: Protein A-Sepharose 4B
attributes.tag: CHIP_ANTIBODY
attributes.value: H3K4me1
attributes.tag: CHIP_PROTOCOL_CHROMATIN_AMOUNT
attributes.value: 0.5 to 3 million cells
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: cell/nuclei lysis
attributes.tag: CHIP_PROTOCOL_ANTIBODY_AMOUNT
attributes.value: ~1 ug
attributes.tag: CHIP_PROTOCOL_BEAD_AMOUNT
attributes.value: 10 ul bed volume
attributes.tag: CHIP_PROTOCOL
attributes.value: Bernstein_BROAD_ENCODE_protocol
attributes.tag: EXTRACTION_PROTOCOL_SONICATION_CYCLES
attributes.value: 1
attributes.tag: CHIP_ANTIBODY_CATALOG
attributes.value: ab8895
attributes.tag: CHIP_ANTIBODY_PROVIDER
attributes.value: Abcam
attributes.tag: CHIP_ANTIBODY_LOT
attributes.value: 659352
attributes.tag: EXPERIMENT_TYPE
attributes.value: Histone H3K4me1
attributes.tag: EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
attributes.value: Branson
attributes.tag: GEO Accession
attributes.value: GSM916024