title: GSM3439846: A508; Mus musculus; OTHER
library_construction_protocol: For whole exome sequencing genomic DNA was extracted from CD4+ T cells, isolated from plck-GAPDH tumors by negative microbead selection (see above), using the Nucleospin Tissue Kit (Macherey Nagel, #740901) Paired-end DNA library was prepared according to the manufacturer's instructions (Agilent). The adapter-modified gDNA fragments were enriched by six cycles of PCR. Whole exome capture was carried out using Agilent's SureSelect All Exon V5 Kit. Finally, 50 Mb of DNA sequences of 33,4378 exons from 20,965 genes were captured. After DNA quality evaluation, pooled samples were sequenced on Illunima Hiseq 2000
attributes.tag: GEO Accession
attributes.value: GSM3439846