title: RNA-seq of cholesteatoma:male adult: Sample 4014_Chol
design: Total RNA was extracted from middle ear cholesteatoma and mucosa tissue samples from patients undergoing surgery for otitis media. Libraries were constructed using the NuGEN Trio RNA-Seq kit (Tecan, Redwood City, CA, USA), which includes an rRNA depletion step. Sequencing was completed on the Illumina NovaSeq, with 40 million reads per sample and paired-end 2x151bp reads.