title: GSM4634744: ZL34_nRBC_553; Macaca mulatta; OTHER
library_construction_protocol: DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN). 200 ng was used for barcode retrieval PCR with Phusion High-Fidel- ity DNA Polymerase (Thermo Fisher Scientific) via 28 cycles of 98_x0014_C for 10 s, 70_x0014_C for 30 s, 72_x0014_C for 30 s, and then 72_x0014_C for 10 min. Indexed or non-indexed forward primers and indexed reverse primers were annealed to the amplified DNA containing the barcode sequence.
attributes.tag: GEO Accession
attributes.value: GSM4634744