title: MiSeq 16S rRNA gene amplicon sequencing of 3 months incubated sample 3
design: PCR amplification of the V4 hyper variable region of the 16S rRNA gene, using a barcoded 515F and 806R primer set, and the FastStart High Fidelity PCR System. The barcoded PCR products were purified and normalized to ~20 ng each, using the SequalPrep Normalization Kit. The products were pooled and sequenced on an Illumina MiSeq platform using version 2 chemistry to produce 2  250-bp pair-end sequencing reads