title: GSM4140460: R5020_12h_run2_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed once in PBS and whole cell lysates were prepared using Trizol reagent (Invitrogen). Total RNA (>200bp) was obtain using RNeasy Plus Mini kit (Qiagen) according to manufacturer specifications. Poly-A-enriched RNA was used to prepare libraries with TruSeq RNA Sample Preparation kit v2 y v4 (ref. RS-122-2001/2, Illumina) according to instructions from manufacturer followed by single-end (run1) and paired-end (run2) sequencing
attributes.tag: GEO Accession
attributes.value: GSM4140460