title: Illumina HiSeq 2000 sequencing; qiita_ptid_31:1481.PO7.2.T0
design: Whole grains contain numerous physiologically bioactive compounds, a key group being polyphenolic compounds (such as ferulic acid). These compounds were shown to have potent antioxidant activity. Limited evidence exists regarding the bioavailability of the whole grain bioactive compounds and their physiological impact on health outcomes in humans such as the CVD risk, body weight and abdominal circumference. Evidence from animal and few human studies indicated that prebiotic dietary fibre ameliorates metabolic syndrome and controls body weight through reduction of inflammation. This study aims i) to evaluate the bioavailability WG polyphenols; ii) the effect of WG on body weight, circumferences and composition, iii) to clarify the underlying mechanisms. A commercial WG product, having prebiotic properties and a high amount of polyphenols bound to dietary fibre, was selected. Eighty healthy overweight subjects will be enrolled. Forty volunteers will be asked to change their habitual diet only replacing equicaloric portions of specific foods with 68 g WG/die for 8 weeks; the other half will include a placebo. At baseline, and after 4 and 8 weeks, measure of body weight, waist and hip circumferences, bioimpedance analysis and blood, urine and feces collection will be performed. Markers linked to antioxidant status (serum ferulic acid, ??-carotene and FRAP), to inflammatory status (several cytokines by multiplexed immunometric assay), to lipid and glucose metabolism as well as to the overall nutritional status and appetite, will be measured.
library_construction_protocol: EMP V4 515f,806rbc protocol
attributes.tag: barcode
attributes.value: ACCAACAGATTG
attributes.tag: center_name
attributes.value: CCME
attributes.tag: center_project_name
attributes.value: Ercolini_whole_grain_feces
attributes.tag: emp_status
attributes.value: EMP
attributes.tag: illumina_technology
attributes.value: HiSeq
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 2012
attributes.tag: run_prefix
attributes.value: Undetermined_S0_L001_sequences
attributes.tag: samp_size
attributes.value: 0.1, gram
attributes.tag: sample_center
attributes.value: Universitaæ degli Studi di Napoli Federico II
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4