title: Illumina HiSeq 4000 paired end sequencing; ChIP-seq of Zic3 in ES-E14Tg2a embryonic stem cells after 2 days of differentiation to epiblast-like cells
design: ChIP-seq of Zic3 in ES-E14Tg2a embryonic stem cells after 2 days of differentiation to epiblast-like cells
library_construction_protocol: ChIPmentaion experiments were performed using ZIC3 (Abcam, ab222124) antibody in the Rex1GFPd2 mouse embryonic stem cell line (ESC; parental line; E14Tg2a) and d1- and d2-EpiLC differentiated states. Cells were grown in 12ng/ml bFGF, 20ng/ml activin A, 1% KOSR on 5ug/ml bovine plasma fibronectin-coated dishes.  ChIPmentation samples and libraries were generated as described previously (Schmidl et al. 2015 https://doi.org/10.1038/nmeth.3542) ChIPmentation  Nextera XT DNA library preparation kit
attributes.tag: Experimental Factor: time
attributes.value: 2
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: anti-Zic3