title: internal transcribed spacer (ITS) region sequences of soil microbiome: forest reference, 0-30 cm depth
design: MoBio PowerSoil DNA Isolation Kit; PCR amplification with fungal primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and 58A2R (5-CTGCGTTCTTCATCGAT-3)