title: GSM1141084: OE1642 mm skin 5months rep1; Mus musculus; RNA-Seq
library_construction_protocol: D. rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater (Qiagen, Hilden, Germany) at 80 °C. Total RNA was isolated with TRIzol (Life Technologies, Darmstadt, Germany) according to the instructions of the manufacturer. M. musculus: The isolated skin was homogenized in 500 μl QIAzol (Qiagen) by subsequently adding 100 μl chloroform. Following phase separation, the aqueous phase was transferred into a fresh tube, then 0.16 volume NaAc (2 M, pH 4.0) and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20 μl water (GIBCO) and stored at –80°C until use. D. rerio: 5 µg of total RNA was used for preparation of libraries using Illumina’s mRNA-Seq sample prep kit (Illumina, San Diego, CA, USA) following the manufacturer’s instruction. M. musculus: 2.5 µg of total RNA was used with Illumina’s TruSeq RNA sample prep kit.
attributes.tag: GEO Accession
attributes.value: GSM1141084