title: GSM2425600: Exo-AT1; Rattus norvegicus; miRNA-Seq
library_construction_protocol: Total RNA from Exo-ADSC and Exo-AT was isolated using TRIzol reagent (Invitrogen, USA) Total RNA of each sample was used to prepare the miRNA sequencing library, which included the following steps:1) 3'-adaptor ligation;2) 5'-adaptor ligation;3) cDNA synthesis;4) PCR  amplification;5) size selection of ~135-155 bp PCR amplified fragments (corresponding to ~15-35 nt small RNAs).The libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 36 cycles on Illumina HiSeq per the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2425600