title: GSM2845439: M17_leaf; Zea mays; RNA-Seq
library_construction_protocol: Total RNA was treated with RQ1 DNase (Promega) to remove DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at 260 nm/280 nm (A260/A280) using Smartspec Plus (BioRad). RNA integrity was further verified by 1.5% agrose gel electrophoresis. For each sample, 5 μg of total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen). Purified mRNAs were fragmented at 95℃ followed by end repair and 5' adaptor ligation. Then mRNA reverse transcription was performed with RT primer harboring 3' adaptor sequence and randomized hexamer. The cDNAs were purified and amplified with RNA-Seq Library Preparation Kit (Gnomegen). And PCR products corresponding to 200-500 bps were purified, quantified and stored at -80 ℃ until before sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2845439