title: RNA-seq of Poecilia reticulata: two adult female brains (control): Sample g1_control_female_2
design: "First generation individuals (g1) in this experiment were either chronically treated with methylphenidate hydrochloride (ritalin) or were controls. This whole-brain gene expression profile corresponds to two sexually-mature, control female guppies (Poecilia reticulata) who were full siblings. Library construction: RNA was extracted from whole guppy brains using Qiagen RNeasy lipid tissue kit. Brains from the two males were pooled pror to extraction. To create cDNA libraries, we used New England Biolabs Ultra RNA library prep kit for Illumina. To allow for multiplexing, we used NEBNext Multiplex Oligos for Illumina. RNA and cDNA were quantified using the appropriate kits for Qubit and checked for quality using an Agilent Bioanalyzer. All kits were used according to the manufacturers' instructions. Four samples per lane were run on the Illumina HiSeq 2000 at the Florida State University sequencing facility."