title: bacterial 16S rRNA (V1-V3) from Sus scrofus fed microbially modified soybean meal : ileal digesta sample M2
design: Sample collected from ileal digesta of weaned pig fed MSBM diet (microbially modified soybean meal as dietary protein source). Sample kept at -20C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using the PowerSoil DNA Isolation Kit (MoBio Laboratory Inc) which included bead beating. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes using 27F-519R primers. Sequencing using Illumina MiSeq2X300.