title: Inoculum from Groesbeek activated sludge in December 2019 replicate 3
design: 16S rRNA gene sequencing was performed on an Illumina Miseq.  The primers used for previous PCR amplification were Bac341F  (5CCTACGGGNGGCWGCAG3) and Bac785R (5 GACTACHVGGGTATCTAATCC 3)  (Klindworth et al., 2013).