title: P. rettgeri infection; RNAi control; Drosophila melanogaster; RNA-Seq
design: Each sample of 20 flies was homogenized, and total RNA was isolated using a modified TRizol extraction protocol (Life Technologies). Following RNA extraction, the 3 end RNA-seq libraries were prepared using QuantSeq 3  mRNA-Seq Library Prep kit (Lexogen). The sample quality was evaluated before and after the library preparation using Fragment Analyzer (Advanced Analytical). Libraries were sequenced on two lanes of the Illumina Nextseq 500 platform using standard protocols for 75bp single-end read.