title: GSM2427818: H150 ISO; Homo sapiens; RNA-Seq
library_construction_protocol: RNeasy Plus Mini Kit (QIAGEN, Cat.no. 74134) was used for total RNA extraction. After extracting the total RNA from samples, mRNA and non-coding RNAs are enriched by removing rRNA from the total RNA with kit. By using the fragmentation buffer, the mRNAs and non-coding RNAs are fragmented into short fragments(about 200-500nt), then the first-strand cDNA is synthesized by random hexamer-primer using the fragments as templates, and dTTP is substituted by dUTP during the synthesis of the second strand. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters, then the second strand is degraded using UNG(Uracil-N-Glycosylase) finally.
attributes.tag: GEO Accession
attributes.value: GSM2427818