title: Cervical Microbiota Associated with Higher Grade Cervical Intraepithelial Neoplasia in Women Infected with High-Risk Human Papillomaviruses
design: Microbial DNA was isolated from cervical mucus samples using the Fecal DNA isolation kit from Zymo Research following the manufacturer's instructions. Once the sample DNA was prepared, PCR was used with unique bar-coded primers to amplify the variable region 4 (V4) region of the 16S rRNA gene to create an amplicon library from individual samples.