title: metagenome sequencing of bioreactor samples (barcoded TruSeq DNA illumina libraries)
design: Preparation of the barcoded illumina TruSeq DNA library was done according to the manual provided by illumina (TruSeq DNA Sample Preparaiton, low throughput). Mechanic fractionation of genomic DNA using Nebulizers (Roche), 6min at 32-35psi, purification with "Qiaquick PCR purification Kit" (Qiagen). Fragment end repair, adenylation of 3' ends and adapter ligation according to TruSeq DNA SAmple Preparation manual. Size selection of library by elution from preparative agarose gel: approx. 300-500bp, i.e. insert size: approx. 180-380bp.