title: GSM4289943: A549_DPP1; Homo sapiens; ncRNA-Seq
library_construction_protocol: The lncRNA libraries were constructed using the rRNA-depleted RNA sample and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA). The divalent cations were used for fragmentation. The synthesis of the first-strand cDNA was performed using M-MuLV reverse transcriptase and a random hexamer primer. The second-strand cDNA synthesis was performed using RNase H and DNA polymerase I. The 3'-end of the DNA fragments was adenylated and ligated with the NEBNext adaptor containing a hairpin-loop structure. AMPure XP system was used to select the cDNA fragments with a size range of 150–200 bp. The cDNA libraries were sequenced on an Illumina HiSeq 4000 platform. Small RNA library was prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina®. Briefly, the NEB 3' SR adaptor was ligated to the 3'-end of miRNAs. The single-stranded DNA adaptor was converted into a double-stranded DNA molecule. The 5'-end adaptor was ligated to the 5'-end of miRNAs. The DNA fragments of size 140–160 bp were dissolved in 8 μL of an elution buffer. The libraries were sequenced on an Illumina HiSeq 2500 platform.
attributes.tag: GEO Accession
attributes.value: GSM4289943