title: Illumina HiSeq 2000 sequencing; Posttranscriptional mRNA expression changes in stress
design: Posttranscriptional mRNA expression changes in stress
library_construction_protocol: Cells were seeded 48 hours prior to stress treatment. Cells were subsequently treated with 5 ??g/ml Anisomycin for 3 hours and harvested in PBS. Total RNA was extracted using Trizol LS. Cells were fractionated and nuclear RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina TruSeq protocol.
attributes.tag: Experimental Factor: Extraction protocol
attributes.value: nuclear
attributes.tag: Experimental Factor: Nucleic acid library construction protocol
attributes.value: standard TruSeq
attributes.tag: Experimental Factor: compound
attributes.value: Anisomycin