title: "RNA-seq of Poecilia reticulata: adult female brain (second generation, dam and sire methylphenidate treated)": Sample g2_tt_female_1
design: "Second generation individuals (g2) in this experiment were the offspring of individuals that were either chronically treated with methylphenidate hydrochloride (ritalin) or were controls. G2 individuals could be the product of parents that were: both treated (tt), both controls (cc), the dam was treated and sire was control (tc), or the dam was control and the sire was treated (ct). This whole-brain gene expression profile corresponds to one sexually-mature, female guppy (Poecilia reticulata) whose parents had been both treated. Library construction: RNA was extracted from whole guppy brains using Qiagen RNeasy lipid tissue kit. To create cDNA libraries, we used New England Biolabs Ultra RNA library prep kit for Illumina. To allow for multiplexing, we used NEBNext Multiplex Oligos for Illumina. RNA and cDNA were quantified using the appropriate kits for Qubit and checked for quality using an Agilent Bioanalyzer. All kits were used according to the manufacturers' instructions. Four samples per lane were run on the Illumina HiSeq 2000 at the Florida State University sequencing facility."