title: Comparative Microbial Analysis to Understand Taxonomic Fingerprints of Healthy Coral Holobiont Communities at southern Great Barrier Reef, Australia.
design: Slapeta J, Linares MC: The tag-encoded FLX amplicon pyrosequencing (TEFAP) was based upon titanium protocols (Roche, Indianapolis, IN) and procedures were performed at the Research and Testing Laboratory (Lubbock, TX; www.researchandtesting.com).  TEFAP was performed using one-step PCR and high fidelity Taq. A total of three eukaryotic 18S rDNA primer sets were utilized; A7F (5’-AAC CTG GTT GAT CCT GCC AGT) and euk-SSU-570R (5’-GCT ATT GGA GCT GGA ATT AC), 516F (5’-GGA GGG CAA GTC TGG T) and 1055R (5’-CGG CCA TGC ACC ACC), 300F (5’- AGG GTT CGA TTC CGG AG) and 555R (5’-GCT GCT GGC ACC AGA CT). Additionally bacterial 16S rDNA assays was evaluated by using same protocols as above with one primer pair; 28F (5’-TTT GAT CNT GGC TCA G) and 519R (5’-GWN TTA CNG CGG CKG CTG). Coral samples tested were from Acropora palifera (42a), Montipora digitata (60a), Porites cylindrica (66-2) and Seriatopora hystrix (176-2).