title: RNA-Seq of Ascaris suum: L3 larvae, batch B, time point 9
design: For transcriptome sequencing on an Illumina platform a TruSeq RNA library generation was utilized. The library was generated by using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) following the manufacturers instructions. The library was quantified by using the KAPA Library Quantification Kit for Illumina (Kapa Biosystems, Wilmington, MA, USA). The library size was determined by using the High Sensitivity DNA Analysis Kit for the 2100 Bioanalyzer Instrument (Agilent Technologies, Waldbronn, Germany). Libraries were adjusted to a concentration of 12 pM and sequenced on a HiSeq 1500 instrument (Illumina, San Diego, CA, USA) in high-output mode. For cluster generation, the TruSeq PE Cluster Kit v3 was used. Cluster generation was performed on a cBot instrument. For sequencing the TruSeq SBS Kit v3 was used to sequence 100 + 100 bases.