title: metagenome_AnnaTempExp_2011-DNaseq-25degC
design: DNA was extracted from 50 mL of pelleted culture. 1 μg of purified DNA per sample was used for the preparation of a whole metagenome shotgun sequencing library according to the “Rapid Library Preparation Method Manual” (May 2011) for GS FLX+ Series provided by Roche (Mannheim, Germany). Library was sequenced on a quarter picotiterplate of a GS FLX+ Titanium sequencing run.