title: GSM1141337: PsyB728a(pBAD::EV)_2; Pseudomonas syringae; RNA-Seq
library_construction_protocol: RNA isolation was performed by using the RNeasy minikit (Qiagen). Isolated total RNA was treated with TURBO DNase (Ambion). Total RNA of each time point was pooled at 1/3 ratio. 10 μg of pooled RNA was depleted of 16S and 23S ribosomal RNA using RiboMinusTM (Invitrogen). Double stranded cDNA were prepared from ~ 1 μg ribosomal depleted RNA, using random primers (Invitrogen). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM1141337