title: GSM4084092: Stat5_Adipoq_basal_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Lipid Tissue Mini Kit (Qiagen, Venlo, Netherlands). RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library amounts were quantified using the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad). For sequencing, libraries were pooled, diluted and sequenced on an Illumina HiSeq 3000 instrument using 50 bp single-read chemistry.
attributes.tag: GEO Accession
attributes.value: GSM4084092