title: GSM4141622: shixiehou_naive; Macaca fascicularis; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen). The scRNA-seq libraries were generated using the Chromium Single Cell 3′Reagent Kit (10X Genomics) . Briefly, a single-cell suspension (500 cells /μL in PBS) was mixed with RT-PCR master mix and loaded together with Single Cell 3′Gel Beads and Partitioning Oil into a Single Cell 3′Chip (10X Genomics) according to the manufacturer's instructions. RNA transcripts from single cells were uniquely barcoded and reverse-transcribed within droplets. cDNA molecules were preamplified and pooled followed by library construction. All libraries were quantified on a 2100 Bioanalyzer (Agilent) and realtime quantitative PCR, and then subjected to 150 bp paired-end sequencing on an Illumina Xten platform (sequenced by Novogene).
attributes.tag: GEO Accession
attributes.value: GSM4141622