title: Illumina HiSeq 2000 sequencing; The impact of Hsp90 upon the Candida albicans transcriptome during heat shock
design: The impact of Hsp90 upon the Candida albicans transcriptome during heat shock
library_construction_protocol: Candida albicans cells (wild type strain SN95 [HSP90/HSP90], and mutant strain CalC1441 [tetO-HSP90/hsp90]) were grown in YPD (2% yeast extract, 2% bactopeptone, 2% glucose) to mid-exponential phase. Cultures were grown either in the absence or presence of 20 g/ml doxycycline.  Cells were then exposed to a 30 C-42 C heat shock, and RNA extracted 10, 30 and 60 minutes post-heat shock.  Untreated controls were included, for which RNA was extracted at time zero before heat shock. Biological triplicate cultures were processed. Cells were flash frozen in liquid nitrogen and RNA was extracted using Qiazol (phenol/chloroform  method). RNA was DNase treated using Ambion Turbo DNAfree kit before being sent for library preparation and sequencing Libraries were prepared using standard Illumina RNAseq library preparation kit.
attributes.tag: Experimental Factor: SN95
attributes.value: strain
attributes.tag: Experimental Factor: doxycycline
attributes.value: growth condition
attributes.tag: Experimental Factor: heat shock
attributes.value: stimulus
attributes.tag: Experimental Factor: 30
attributes.value: time