title: Shifts in microbial community structure and function in light- and dark-grown biofilms driven by warming
design: Biofilm samples from three independent laboratory microcosms grown at high temperature cycle (17.2*0.5*C (day) and 16.5*0.2*C (night)) and under illumination (150  mol photons m-2 s-1 for 12 h/day) were subjected to DNA extraction using  UltraClean  Soil DNA Extraction Kit (MoBio Laboratories Inc.) according to manufacturer instructions. DNA from each replica were pooled in equimolar ratios and used as template in PCR reactions using primers 357F/907R targeting the V3/5 region of the bacterial 16S rRNA gene complemented with 454-adapters and sample-specific barcodes. Pyrosequencing was performed in a Roche Genome Sequencer 454 GS FLX Titanium using optimized protocols developed by the sequencing company (Research and Testing Laboratory, Lubbock, TX).