title: Survey of transcriptomes  Brevibacillus borstelensis in response to low and high temperature shock
design: Bacterial culture of Brevibacillus borstelensis was grown overnight in NB media and RNA was isolated from cells heat shocked at 55 degree cellsius for 5 minutes. Ribodepleted total RNA(mRNA) was isolated and then fragmented using RNase III enzyme. Fragmented mRNA was hybridized with adapters followed by ligation. Reverse transcription was carried out to obtain cDNA and then size selected libraries were amplified. Finally transcriptome library was quantified and qualified using bioanalyzer.