title: GSM1138985: C4; Homo sapiens; ChIP-Seq
library_construction_protocol: Lymphocytes were isolated from 10 ml Heparin blood using a swelling buffer (155 mM NH4CL2, 10 mM KHCO3, 0,01 mM EDTA) to disrupt erythrocytes. Cells were cross-linked in 1% formaldehyde for 10 min at room temperature. Chromatin was sonicated using a Bioruptor sonicator (Diagenode, Liège, Belgium) for 28 minutes at high power, 30 s ON, 30 s OFF, and ChIP was performed using a CTCF antibody (Millipore, Billerica, MA, USA; 07-729). 7 ng of DNA as measured by Qubit (Life Technologies) was prepared for sequencing. A barcoded NEXTflex adaptor was ligated to the DNA fragments, and fragments with adaptors were amplified by a pre-size selection PCR of 4 cycles, followed by size selection (∼300 bp) and further amplification by 14 PCR cycles. Three barcoded samples were sequenced in one lane on a HiSeq 2000 (Illumina, San Diego, CA, USA). The reads were demultiplexed using CASAVA 1.8.2 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM1138985