title: Bisulfite-Seq analysis of RRBS_Lib 273 derived from human anterior caudate cells
design: Smith et al., Methods 48, 226-232
attributes.tag: BISULFITE_CONVERSION_PROTOCOL
attributes.value: 2x5hrs Epitect Kit
attributes.tag: DNA_PREPARATION_INITIAL_DNA_QNTY
attributes.value: 30-300 ng
attributes.tag: EXTRACTION_PROTOCOL_SONICATION_CYCLES
attributes.value: NA
attributes.tag: DNA_PREPARATION_ADAPTOR_LIGATION_PROTOCOL
attributes.value: T4 Ligase (2,000U/ul) 16C o/n
attributes.tag: DNA_PREPARATION_ADAPTOR_SEQUENCE
attributes.value: Illumina TruSeq adapter
attributes.tag: LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
attributes.value: Illumina TruSeq primer
attributes.tag: DNA_PREPARATION_POST-LIGATION_FRAGMENT_SIZE_SELECTION
attributes.value: 120-400
attributes.tag: BISULFITE_CONVERSION_PERCENT
attributes.value: 99.5%
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: Standard Protocol (Smith et al., Methods 48, 226-232)
attributes.tag: LIBRARY_GENERATION_PCR_PRIMER_CONC
attributes.value: 25uM
attributes.tag: DNA_PREPARATION_FRAGMENT_SIZE_RANGE
attributes.value: 30-280
attributes.tag: EXPERIMENT_TYPE
attributes.value: DNA Methylation
attributes.tag: LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
attributes.value: Smith et al., Methods 48, 226-232
attributes.tag: LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
attributes.value: SPRI Beads
attributes.tag: LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
attributes.value: Illumina TruSeq primer pair
attributes.tag: LIBRARY_GENERATION_PCR_TEMPLATE_CONC
attributes.value: NA
attributes.tag: LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
attributes.value: Pfu Turbo Cx hot start
attributes.tag: LIBRARY_GENERATION_PCR_NUMBER_CYCLES
attributes.value: 11-15
attributes.tag: EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
attributes.value: NA
attributes.tag: GEO Accession
attributes.value: GSM916079