title: RNA-seq of Achyranthes  bidentata roots:the seed-setting stage
design: The root RNA samples were used to construct the sRNA-seq libraries (85-DAP sample only). An equal amount of total RNA per sample (3 g) was used as input material for the sRNA library. Sequencing libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA), following the manufacturers recommendations, and index codes were added to attribute identifiable sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster. Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumina), according to the manufacturers instructions.