title: GSM4138588: RNA-seq, wild-type Ago2Halo/+ mESCs, biological rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were extracted from cells using TRIzol Reagent following the manufacturer's instructions , treated with DNase and cleaned up using the Qiagen RNeasy kit. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR. Samples were barcoded and run on a  HiSeq 2500 in High Output mode  in a 50bp/50bp paired end run, using the TruSeq SBS Kit v4 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4138588