title: 2-16S amplicon: corn silage
design: Silage samples were collected, and grinded to powder in liquid nitrogen. DNA were extracted from the silage  samples, and PCR amplification of 16S rRNA gene fragments was performed using 515F and 806R primers. PCR amplicon was sequenced using Ion Torrent PGM. Raw reads were sorted  by barcodes. Barcode and  primer sequences were trimmed, and data was analyzed using qiime pipeline version 1.91  to filter out low quality sequences (qual below 20) and short reads (length below 250bp).