title: Transcriptome analysis of Aedes aegypti developing in brackish waters
design: For library construction, DNA/RNA is extracted from a sample. After performing qualitycontrol(QC), passed sample is proceeded with the library construction. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followedby 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligationreactions into a single step that greatly increases the efficiency of the library preparation process.Adapter-ligated fragments are then PCR amplified and gel purified. For cluster generation, the library is loaded into a flow cell where fragments are captured on a lawnof surface-bound oligos complementary to the library adapters. Each fragment is then amplifiedinto distinct, clonal clusters through bridge amplification. When cluster generation is complete, thetemplates are ready for sequencing.