title: GSM4138586: RNA-seq, AgoHalo/+ mice; normal lung, biological rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Lung tumors or normal lung tissues were resected from mice and homogenized in TRIzol Reagent. Total RNA were extracted following the manufacturer's instructions, treated with DNase and cleaned up using the Qiagen RNeasy kit. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit.
attributes.tag: GEO Accession
attributes.value: GSM4138586