title: GSM2345408: co-culture treated with Bic/4AP for 1 h Hs+Ms_Bic-4AP_1h_2; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from cultured cells (3.5 cm dish) with RNeasy Mini (hiPSCd neuron/mouse primary neuron co-cultures) or Micro (hiPSCd neuron-only cultures) Kit (Qiagen) along with on-column RNase-Free DNase (Qiagen) digestion of DNA Poly(A)+ RNA was isolated from total RNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). NEBNext Multiplex Oligos for Illumina (New England Biolabs) were used.
attributes.tag: GEO Accession
attributes.value: GSM2345408