title: GSM2425430: Dawn + 10.5h Rep 3; Oryza sativa; RNA-Seq
library_construction_protocol: Tissue samples were stored at -80C until needed. The panicle samples were first ground in liquid nitrogen with a metal pestle. The tissue then lyophilized at -60C overnight before RNA extractions.Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen) with the recommended RLT lysis buffer. The RNA extraction provided protocol was followed the inclusion of DNase step. RNA concentration was then measured with NANOdrop 2000. 2ug of RNA was used with NEBNext Poly(A) Magnetic mRNA isolation kit (NEB). Oligo(dT) attached to magnetic beads isolate mRNA by attaching to poly(A) modified mRNA Libraries were constructed using NEB ultra dircetional library preperation kit. Library quantification was done with both NEB library qRT quantification and bioanalyzers
attributes.tag: GEO Accession
attributes.value: GSM2425430