title: GSM4140481: ATAC_T0; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were lyzed with 50ul cold lysis buffer (Tris-Cl pH 7.4 10mM; NaCl 10mM; MgCl2 3mM; NP-40 0.1% v/v) and centrifuged at 500xg for 10min at 4ºC. Nuclei were resuspended in TD Buffer with 1.5ul Tn5 Transposase (Nextera, Illumina) and incubated 15 minutes at 37ºC. DNA was isolated using Qiagen MinElute column. Purified DNA was submitted to 10 cycles of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (Univ. primer: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG ; Indexed primers: CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTCTCGTGGGCTCGGAGATGT). Library were size selected using AMPure XP beads and sequenced on a NextSeq 500 instrument (2x75 nt).
attributes.tag: GEO Accession
attributes.value: GSM4140481