title: DNA-Seq of coastal hydrothermal sediment: open incubation
design: To explore autotrophy at a shallow-water hydrothermal system off Milos (Greece), sediment was extracted with RNeasy PowerSoil Total RNA Kit (Qiagen). DNA was simultaneously extracted with RNeasy PowerSoil DNA Elution Kit (Qiagen). The V4 region of the 16S rRNA gene was targeted with primers 515F and 806R in a single-step 30 cycle PCR.