title: GSM4291820: Ethanol_Ampicillin_3; Escherichia coli; RNA-Seq
library_construction_protocol: Total RNA was extracted from the bacterial samples using the RNeasy mini kit (Qiagen), and any possible DNA contamination was removed by performing on-column DNAse digestion (Qiagen). mRNA was enriched by removing ribosomal RNA using the capture oligonucleotide mix (MICROBExpress, Ambion). RNA-Seq libraries were prepared using the KAPA Stranded RNA-Seq Library Preparation Kit for Illumina platforms (Kapa Biosystems), and instructions suggested by the manufacturer were followed. Double size selection of libraries was performed using Agencourt AMPure XP magnetic beads (Beckman Coulter), where cDNA fragments ranging from 200-500 bp were enriched. The DNA concentration of the final pooled library was determined with Bioanalyzer DNA high sensitivity assay (Agilent, DNA Technologies Core, UC Davis). Sequencing was performed with HiSeq4000 SR50 (DNA Technologies Core, UC Davis).
attributes.tag: GEO Accession
attributes.value: GSM4291820