title: RNAseq of Homo Sapiens: male
design: Total RNA was extracted from cells restimulated for 16 h for RNA-seq analysis (Qiagen). In brief, 100 ng of total RNA was used to generate RNA-Seq libraries with the Illumina TruSeq stranded mRNA LT kit (Cat# RS-122-2101).   Libraries prepared with unique barcodes were pooled at equal molar ratios. The pool was denatured and sequenced on an Illumina NextSeq 500 sequencer with high-output V2 reagents and NextSeq Control Software v1.4 to generate 75 bp single reads, according to the manufacturers protocol (Illumina, Cat# 15048776).