title: RNA-Seq of mus musculus: adult female heart
design: RNA extraction and purification of all samples was done using the RNeasy Micro Kit according to manufacturer's instructions. An illumina TruSeq Stranded Total RNA kit was used to prepare the libraries for poly-A enrichment of mRNA. For sample amplification, 15 PCR cycles were used, standard to the Illumina kit protocol. RNA sequencing was performed on an Illumina NextSeq500. Each library was paired-end with 75bp reads, as well as 20 million reads per sample.