title: Coffea arabica/Hemileia vastatrix 454 GS-FLEX Titanium
design: The Transcriptome of Coffea arabica leaves infected by the rust fungus Hemileia vastatrix at 21 days after inoculation. The aim of this study was to identify transcripts from the coffee plant and the coffee rust fungus expressed during the interaction from plant tissue infected by the fungus. A goal was to determine the origin of the transcripts (plant or fungus) using comparison to extrinsic and intrinsic databases.
library_construction_protocol: Coffea arabica CIFC H147/1plants were grown in glasshouses. Fully developped coffee leaves were inoculated with fresh urediniospores of H. vastatrix CIFC isolate 178a, and collected 21 days after inoculation and snap-frozen in liquid nitrogen. RNA isolation was carried out on the infected leaf samples using the RNeasy Plant Mini Kit (Qiagen) completed by a DNase treatment as recommended by the manufacturer. cDNA were synthetized using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer's instructions and purified using the Qiaquick PCR Purification Kit (Qiagen).