title: Whole genome sequencing of Calanus finmarchicus for development of nuclear insertion/deletion markers
design: Whole organism of Calanus finmarchicus, female, was sequenced in order to obtain genomic sequences for development of insertion/deletion markers between C. finmarchicus and C. glacialis. The Ion Plus Fragment Library Kit (Life Technologies) was used for the construction of 200 bp library. Emulsion PCR was done in the Ion One Touch System, using the Ion One Touch 200 Template Kit. Sequencing was performed in the Ion Personal Genome Machine (PGM™) System using the Ion PGM 200 Sequencing Kit and an Ion 316 chip.