title: GSM4138672: RNAseq_RT4_Ni_exposed_Dup; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy Kit (Qiagen 74104). ChIP was performed using samples isolated from two biological replicates. The cells were crosslinked with 1% formaldehyde for 10 minutes at 25°C and sonicated to obtain 200-500bp fragments. ChIP was performed as described earlier using ChIP grade antibodies against H3K4me3 (Millipore, 07-473). RNA-Seq libraries were prepared using Illumina TruSeq RNA Sample Preparation Kit (RS-122-2002) according to manufacturer's protocol. ChIP-Seq libraries were prepared using Illumina TruSeq ChIP sample preparation Kit (IP -202-1024), according to manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM4138672