title: healthy_tomato_tissue_6421
design: Tomato (Solanum lycopersicum) plants were grown in the greenhouse under standard conditions. Transgenic tomatoes SlPH are impaired of the PH ortholog controls fruit acidity. Fruit juice of the silenced plants had nearly a 2 pH units increase compared with the non-silenced plants, together with reduced % acid. Tomato fruits were inoculated by placing 5 μL of a conidial suspension containing 106 spores mL-1 on each of four 2-mm-deep, 2-mm-diameter wounds spaced evenly in a circle around the upper part of the stem end of 8 fruits. Following inoculation, the fruits were incubated for 3 days at 25°C in covered plastic containers containing wet paper towels. Samples of healthy and infected tissues were collected and frozen with liquid nitrogen for subsequent RNA analysis.