title: GSM4141604: RNA_SCNT_Late2C_rep1; Mus musculus; RNA-Seq
library_construction_protocol: The RNA extraction follows smart-seq2 protocol The RNA-seq libraries were generated using the Smart-seq2 protocol as described previously with minor modification (Picelli et al., 2014). Cells were lysed in hypotonic lysis buffer (Amresco, M334), and the polyadenylated mRNAs were captured by the PolyT primers. After ~3–10 min lysis at 72 °C, the Smart-seq2 reverse transcription reactions were performed. After pre-amplification and AMPure XP beads purification, cDNAs were sheared by Covaris and were subject to Illumina TruSeq library preparation. All libraries were sequenced on Illumina HiSeq 2500 according to the manufacturer's instruction. Nextera
attributes.tag: GEO Accession
attributes.value: GSM4141604