title: 16S Amplicon of Rheobates palmatus: skin epidermis
design: DNA for microbial community analysis was extracted from each of 54 samples (44 from frogs and 9 from water) using the Qiagen DNeasy blood & tissue kit (Valencia, California, USA) following the manufacturers protocol slightly modified. We obtain 16S rRNA gene amplicons amplifying the V3 and V4 regions obtaining a single amplicon of approximately 460 bp.