title: GSM1135047: OIS Ras-Induced H3K4me3; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes.  Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C.  ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample.  30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures.  Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively).
attributes.tag: GEO Accession
attributes.value: GSM1135047