title: GSM1141065: EPD1; Caulobacter vibrioides; RNA-Seq
library_construction_protocol: Total bacterial RNA was isolated using phenol-chloroform extraction.  The quality of the extracted RNA was assessed by agarose electrophoresis; rRNA bands appeared intact and no RNA smear was apparent. RNA samples were immediately frozen and stored at -80oC. RNA samples were later enriched for mRNA using the Invitrogen Ribominus Transcriptome Isolation Kit (Yeast and Bacteria) to remove ribosomal RNA per the manufacturer’s protocols except for the use of custom-made nucleic acid probes (Invitrogen) designed against C. crescentus ribosomal sequences.  All RNA samples were tested for integrity on a BioRad Experion capillary electrophoresis system. Possible residual DNA was removed by addition of Ambion Turbo DNase. Fifteen sequencing libraries for Applied Biosystems SOLiD system sequencing were created using the Applied Biosystems Whole Transcriptome Library Preparation for SOLiD Sequencing, and individual samples were barcoded using Applied Biosystems Small RNA Expression Kit (SREK) barcodes (per the manufacturer’s protocols).  Transcriptome library preparation was performed for labeling in a strand-specific manner.
attributes.tag: GEO Accession
attributes.value: GSM1141065