title: GSM3439076: pDC PU.1 ChIP-seq Rep 2; Mus musculus; ChIP-Seq
library_construction_protocol: 10^7 cells were crosslinked 1% paraformaldehyde (Sigma-Aldrich) in PBS then lysed (1%SDS + 1mM EDTA + proteases inhibitors). Cross-linked DNA was sonicated with the Bioruptor (Diagenode). Protein G Dynabeads (Invitrogen) were incubated with 20 μg of PU.1 antibody (Santa Cruz, T-20) according to the manufacturer's protocol. Coupled antibody was added to 100 μg of chromatin and incubated overnight at 4°C. Unbound chromatin was removed using a series of four washes. Following elution, bound chromatin was reverse cross-linked and subjected phenol/chloroform immunoprecipitation. Libraries were prepared at the Beijing Genomic Institute (BGI) according to Illumina's instructions and standard BGI ChIP-seq protocols.
attributes.tag: GEO Accession
attributes.value: GSM3439076