title: GSM4291414: Allowtwaplko1 [Sample_116513]; Mus musculus; RNA-Seq
library_construction_protocol: On day +7 post HSCT, donor splenic T-cells were harvested and enriched using the Pan T-cell Isolation Kit II (Miltenyi Biotec).  Live donor T-cells were subsequently flow sorted (LiveDead NearIR-/CD3-PE+/CD45.2-APC+).  RNA was extracted using the AllPrep DNA/RNA Mini Kit with on-column DNaseI digestion from Qiagen. Libraries were constructed using 100ng of total RNA and the KAPA RNA HyperPrep Kit with  RiboErase per manufacturer instruction.
attributes.tag: GEO Accession
attributes.value: GSM4291414