title: C_BT7_12W
design: RNA samples with RIN values  greater than 7 were processed for downstream library preparation and  sequencing. Briefly, 500 ng total RNA per sample extracted from the posterior  kidney, were used for library preparation with the TruSeq RNA Library Prep  Kit v2 (Illumina) according to the manufacturers protocol. Library quality  control was done with the D1000 ScreenTape assay (Agilent, Santa Clara, CA,  USA) on the 4200 TapeStation. Sequencing of libraries (100 base pair single  read) were performed on llumina HiSeq 2500 platform.