title: GSM4140781: HEC-1-B_scRNA_30; Homo sapiens; RNA-Seq
library_construction_protocol: Trypsin was added to the culture dish and the single cells were suspended in the culture medium. Single cells were then picked under the microscope by using a micro capillary pipette into the thin-walled PCR tube containing 2 µl of cell lysis buffer, 1 µl of oligo-dT primer and 1 µl of dNTP mix. First, reverse transcription is performed, the cDNA was pre-amplification by PCR. The cDNA library was then purified, tagmented, and ligated with adapters using the Nextera XT DNA Library Preparation kit (Illumina FC-131-1096). Finally, the adapter-ligated fragments were further amplified by PCR and purified with AMPure XP beads (Beckman).
attributes.tag: GEO Accession
attributes.value: GSM4140781