title: Illumina HiSeq 2500 sequencing; RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells
design: RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells
library_construction_protocol: & days differentiated Th1 and Th2 cell were re-stimulated over night with anti CD3 coated flasks. The cells were removed with centrifugation and extracellular vesicles were isolated using differential centrifugation.  RNA was isolated and purified using Norgen miRNA isolation kit. mirna library was constructed using diagenode small rna kit
attributes.tag: Experimental Factor: stimulus
attributes.value: Th2 differentiation and re-stimulation
attributes.tag: Experimental Factor: protocol
attributes.value: RNA-Seq