title: S635FS biological replicate 3
design: Two-month-old cardiomyocytes from 3 independent differentiation experiments were collected, and RNA was purified using TRizol extraction and isopropanol precipitation. Total RNA (2-10 ng) was reverse transcribed to generate full-length complementary DNA (cDNA) using SmartSeq2. Then, 100-200 pg of full-length cDNA was tagmented using Illuminas Nextera XT kit to generate fragments with a median length of 500 bp for the addition of the sequencing index adapters as described by the manufacturers protocol. Multiple samples with different sequencing indexes were pooled and sequenced on multiple Illumina HiSeq lanes at an average depth of 100 million reads/sample.