title: GSM4008310: 12hr_hpi_b2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extractions were performed in accordance with recommended protocols (Invitrogen, #15596026). Libraries were constructed from 500 ng of total RNA using the SMARTer™ PCR cDNA Synthesis Kit (Clontech, #634926) and Advantage 2 PCR kit (Clontech, #639207) followed by Tn5-mediated tagmentation (see Methods and Materials for an in-depth protocol).