title: GSM4083341: smC72: Small RNA Cells ATG7-/- Biorep #2; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA from wild-type, ATG7-/- and ATG12-/- HEK293T EVs and cells was isolated using the Macherey-Nagel NucleoSpin kit (# 740971) according to the manufacturer's protocol. Small RNA libraries were prepared using the SMARTer smRNA-Seq Kit for Illumina (Takara #635030), libraries were amplified for 12 cycles and subject to size selection using SPRI AMPure Beads (Beckman Coulter, #A63880). RNA-seq: Single-end 50 bp read length on a HiSeq4000
attributes.tag: GEO Accession
attributes.value: GSM4083341