title: GTSeq of AOK-081
design: These reads represent amplicons from a multiplexed PCR of 160 SNP loci, following in general the technique of GT-Seq (Campbell et al. 2015). Contact the author for a list of primers used in the amplification, size of the amplicons, position of the SNP loci in teh amplicon, adapter sequences ligated to the primers, barcoding strategy, and thermal cycling parameters.  To generate these sequence data, the primer pairs were diluted initially into a 5uM pool. The final concentration of primers was 0.054 uM in a 10 ul PCR reaction containing template for each individual fish separately (each fish had its own 10 ul PCR).  Each library contains amplicons for that respective fish, and were 300 bp paired end sequenced. All amplicons were less than 450 bp, and read pairs should overlap.