title: GSM2428966: moDCs of PBS2; Mus musculus; RNA-Seq
library_construction_protocol: RNeasy Plus Micro kit (Qiagen) was used according manufaturer's protocol to extract RNA from sorted moDCs. Quantity and quality of total RNA was assessed by Bionanalyzer (Agilent). All samples had a RIN≥8. Amplification of cDNA from total RNA was performed using the Ovation RNA-Seq system V2 (Nugen). Then, cDNA was fragmented and ligated into a sequencing library using Ovation(R) Ultralow Library System V2 | NuGEN. Libraries were PCR amplified for 12 cycles and sequenced on an Illumina NextSeq 500 System with TruSeq reagent kits and software, generating single-end 150 base-pair reads.
attributes.tag: GEO Accession
attributes.value: GSM2428966