title: GSM4683961: HD5 cDNT (CD3+TCRab+CD4-CD8- NOT CD38+CD45RA+); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the Qiagen RNAeasy microkit Barcoded RNA-sequencing libraries were prepared from 100 ng total RNA using the Ovation Human RNA-Seq system for small amounts of RNA (Nugen, San Carlos, CA). For selective depletion of rRNAs, the insert-dependent adapter cleavage method from Nugen was used prior to cDNA synthesis. To control sources of variability during sample and data processing, a common set of external spike-in RNA controls, developed by the external RNA controls consortium (ERCC), was used (Ambion).
attributes.tag: GEO Accession
attributes.value: GSM4683961