title: GSM2855044: WT Rep3; Listeria monocytogenes EGD-e; RNA-Seq
library_construction_protocol: Total RNAs were extracted by the phenol-chlorophorm method and enriched for mRNAs before sequencing. Sequencing of mRNAs was performed using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and quality control for RNA fragmentation and library construction was assessed by Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and 2200 TapeStation (Agilent) analyses. Template preparation was achieved with an Ion Chef system (Thermo Fisher Scientific) and sample sequencing was done in triplicates with an Ion Proton System (Thermo Fisher Scientific).