title: mESC_dual_mRNA_sc3
design: A single cell was lysed with 200 ng carrier RNA mixture (containing                 100 ng 5'-phosphorylated small carrier RNA mimic). The poly-A RNAs were selected                 using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), and the small                 RNAs remained in the supernatant after poly-A selection. The poly(A) mRNA sequencing                 library was constructed following the manufacturer's protocol using a NEBNext kit                 (NEB #E7530). After adaptor ligation and USER digestion, cDNA fragments from the RNA                 carrier were removed by Not I digestion. After Not I digestion, the library DNA was                 purified for PCR amplification.