title: GSM3864960: 48hrs infected; Homo sapiens; FAIRE-seq
library_construction_protocol: FAIRE analysis of Chlamydia trachomatis-infected and mock-infected HEp-2 cells was performed according to the protocol published by Simon et al (Simon JM, Giresi PG, Davis IJ, Lieb JD: Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to  isolate active regulatory DNA. Nat Protoc 2012, 7(2):256-267), including crosslinking with formaldehyde, cell lysis, phenol-chloroform extraction, reverse-crosslinking, and purification prior to library construction. FAIRE-Seq libraries were prepared from 10-100ng crosslinked DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) as per the manufacturer's protocol