title: GSM3498612: shLuc_MNase_ESC; Mus musculus; MNase-Seq
library_construction_protocol: Cells were crosslinked with formaldehyde and subsequently treated with MNase. Mononucleosome sized fragments of MNase-digested DNA was subjected to library preparation. MNase-enriched DNA was end-repaired using the End-It DNA End-Repair kit (Epicentre), followed by addition of a single A nucleotide, and ligation of custom Illumina adapters. PCR was performed using Phusion High Fidelity PCR master mix