title: RNA-seq high LP in Neurospora crassa
design: Cultures were grown 36 hours in light and transferred to constant darkness for 24 hours. 1 minute high LP is given and cultures were harvested 0, 5, 15, 30, 45, 60, 90, 120 min after LP. Total RNA was extracted by Triazol reagent and mRNA libraries were prepared with NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in combination with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490)