title: Grassland invaders and their mycorrhizal symbionts: a study across an ecotone and an invasion gradient-NOMD
design: "DNA from freeze-dried, ground roots (15 mg) was extracted using MoBio PowerPlant DNA isolation kit. The template was amplified with the LSU rRNA primer set FLR3 and FLR4  which targets the nLSU-D2 region. We added linkers A and B, and MID-tag sequences (one for each plant community) to primers according to Roche recommendations. Sub-samples within plant communities were extracted and amplified separately and pooled based on band intensity run on a 2% agarose gel. We purified the 425-475bp bands using the QIAEX II kit. Samples from all plant communities  (all with different MID-tags) were combined prior to sequencing based on DNA concentrations (nanodrop) to ensure similar representations from all sites prior to sequencing at the Genome Sequencing and Analysis Facility at Univ. of Texas Austin. "