title: Gpaniculata Microsatelite Library
design: DNA from five individual G. paniculata was extractd. DNA was pooled and digested using restriction enzymed and ligated using an Illuimna Y-adaptor. Digested, ligated DNA fragments were enriched for microsatellites by hybridization to 3 -biotinylated oligonucleotide repeat probes. Enriched fragments were captured by streptavidin-coated magnetic beads, and fragments were amplified with One Taq polymerase and a pair of Illumina primers (one universal, one index). Equal amounts of each library were pooled and fragments 300-600 bp were recovered with Ampure beads