title: RNA-seq of ileal tissue from germ-free mouse 8 control replicate 2
design: Ileal tissue was collected in RNALater reagent for storage. RNA isolation from tissue was performed with QIAzol Lysis Reagent and Qiagen RNeasy Plus Universal Mini Kit with gDNA Eliminator Solution.  Total RNA quality was assessed using an Agilent 2100 Bioanalyzer and sent to UIUC for RNA-sequencing.