title: Illumina HiSeq 1500 sequencing; RNA-sequencing of the small-RNA transcriptome in blood, across three time-points in severe psoriasis patients treated with the tumor necrosis factor (TNF) inhibitor, etanercept
design: RNA-sequencing of the small-RNA transcriptome in blood, across three time-points in severe psoriasis patients treated with the tumor necrosis factor (TNF) inhibitor, etanercept
library_construction_protocol: Whole blood was taken at each participant visit, each participant had had at least two PAXgene tubes of whole blood collected for RNA extraction.  All patients were given etanercept subcutaneous administration 25mg twice weekly (administered either as 25mg twice weekly or 50mg once weekly).  miRNA were extracted from blood using miRNeasy blood kits according to manufacturers' protocol. RNA was quantified and quality controlled (assuring a RIN>8) using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Waldbronn, Germany) using the manufacturer's protocol.   TruSeq Small RNA library preparation (Illumina)