title: mRNA-Seq of PBMCs from patients with acute-on-chronic hepatic dysfunction
design: PBMCs were collected from patients and volunteers using Ficoll-paqueTM PLUS medium and total RNA was extracted using TRIzol reagent. Sequencing libraries were prepared following the manufacturers instructions. The pooled library consisted of sequences with lengths of approximately 250 nucleotides. The library was sequenced using the HiSeq 2500 sequencing system.