title: RNA-Seq of Methanosarcina barkeri str. MS
design: rRNA depletion from total RNA using Ribo-Zero Bacterial rRNA removal kit (Illumina, Inc., San Diego, CA); bi-directional librar prep using Nextera DNA Flex Library Kit (Illumina) on automated Apollo 324 system (Takara Bio USA, Mountain View, CA); RNA-Seq for 318 cycles on two lanes of a NovaSeq SP Flowcell (1 x 150 bp) at Princeton University core genomics facility