title: GSM3030500: loi_2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from heart tissue using RNeasy Plus Mini kit (Qiagen). Samples with RNA Integrity numbers >9 were used for sequencing. Ribosomal RNA was depleted from 1-5 μg total RNA using the RiboZero Gold kit (Illumina). Library preparation for the Illumina HiSeq2500 was performed using the TruSeq RNA Sample Prep V2 kit (Illumina). Pooled barcoded libraries were sequenced to generate paired-end 101 bp reads.