title: GSM3181714: WT Adult Ileum Villus RNA-seq replicate1; Mus musculus; RNA-Seq
library_construction_protocol: Villi from distal 1/3rd (ileum) of mouse small intestine were isolated by incubating in 5mM EDTA in PBS for 45 minutes at 4°C. Cells were used immediately for RNA extraction. Total RNA from cells collected in TRIzol reagent (Thermo Fisher Sci.) was extracted with the RNeasy kit (Qiagen) and Dnase treatment. The TruSeq RNA Sample Preparation Kit V2 (Illumina) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.