title: GSM4141781: Testis, Control  3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Left testis were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis Total RNA of each sample was used to prepare the RNA sequencing library 1)RNA fragment;2)Random primed first strand cDNA synthesis;3)dUTP based second strand cDNA synthesis; 4)Adaptor ligation and PCR amplification.