title: amplicon sequencing of river water of shuikou reservoir
design: The universal primer pair 515F (5'-GTG YCA GCM GCC GCG GTA-3') and 907R (5'-CCG YCA ATT YMT TTR AGT TT-3') was employed to amplify the V4-V5 region of prokaryotic 16S rRNA genes. The PCR condition used was 95C for 3 min, 25 cycles of 95C for 45s, 50C for 60s, 72C for 90s and lastly 72C for 10 min