title: mESC_smart_1ng_3
design: cDNA from 1 ng of total mESC RNA was produced following the                 manufacturer's protocol using the Smart-Seq2 v4 Ultra Low Input RNA kit (Clontech)                 for single cells. Then, 2 ng of cDNA was sheared into 150- to 350-bp fragments by                 sonication, and a NEBNext DNA library preparation kit (E7645) was used to construct                 the sequencing library.