title: Diversity assessments from microbes to mammals with eDNA metabarcoding in the Monterey Bay National Marine Sanctuar
design: This protocol is aimed at amplifying the 16S rRNA hypervariable regions 4 & 5 (16S V4&V5) in prokaryotes. The primers (515F-Y and 926R) utilized in this protocol are based on the primers from Aprill et al 2015 and Parada et al 2016.