title: In solution hybrid selection of 503 candidate genes in Lolium perenne
design: An indexed shotgun sequencing library was prepared from 100 ng CTAB extracted genomic DNA by (i) Adaptive Focused Acoustic (AFA) fragmentation on a Covaris S2 instrument (Covaris, Inc.), (ii) adapter ligation, and (iii) magnetic bead purification using a slightly adapted version of the protocol of Uitdewilligen et al., 2013 (https://doi.org/10.1371/journal.pone.0062355). 96 libraries were pooled without normalization and used for probe capture hybridization reaction according to the SureSelect protocol (Agilent SureSelectXT2 Target Enrichment for Illumina Paired-End Sequencing Library Protocol, v. 1.0) and PCR amplified and sequenced on a HiSeq2000 using 91 PE sequencing (BGI, Shenzhen, China).