title: NextSeq 550 sequencing; RNA-seq of JAK2VF and DNMT3A mutant Myelofibrosis patients
design: RNA-seq of JAK2VF and DNMT3A mutant Myelofibrosis patients
library_construction_protocol: Peripheral blood was collected from patients with primary myelofibrosis (PMF) or post ET- or PV-myelofibrosis (MF). Patients were part of of  the MPNSG-0109 study (Pomalidomide in myeloproliferative neoplasm-associated myelofibrosis. Schlenk RF, Stegelmann F et al., Leukemia 2017). HREC approval 45/07 Universität Ulm Ethikkommission. Most patients were untreated at sample collection, apart from two patients treated with Pomalidomide and one patient treated with Hydroxyurea. RNA was extracted  RNA libraries were prepared using the NEBnext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), assessed for size and quantified using the 2100 Bioanalyzer (Agilent Technologies) and Kapa Library Quantitation Kit (Illumina) respectively.