title: GSM4086344: Tree_08_Tolf_T2; Citrus sinensis; RNA-Seq
library_construction_protocol: Fifty leaves were collected randomly from the canopy of each tree. Following collection, the midrib and petiole of each leaf was excised and immediately submerged in RNAlater. Prior to RNA extraction, the tissue samples were freeze (-80°C), dried and homogenized.  25 mg of each powdered leaf was used. Total RNA was extracted with Isolate II RNA Plant Kit (Bioline, Taunton, MA). RNA integration number (RIN) was measured using BioAnalyzer (Agilent Technologies) RNA 6000 Nano Kit. TruSeq Stranded total RNA with Ribo-Zero Plan Kit (Illumina)