title: GSM1145401: shLuc_4TF-MEF_Input_48h; Mus musculus; ChIP-Seq
library_construction_protocol: 4TF-MEFs were crosslinked with formaldehyde and subsequently sonicated. Sonicated cell extracts were used for ChIP assays. ChIP-enriched DNA was end-repaired using the End-It DNA End-Repair kit (Epicentre), followed by addition of a single A nucleotide, and ligation of PE adapters (Illumina) or custom indexed adapters. PCR was performed using Phusion High Fidelity PCR master mix