title: Illumina HiSeq 2000 paired end sequencing; ChIP-Seq analysis of CCCTC-binding factor (CTCF) binding in rodents
design: ChIP-Seq analysis of CCCTC-binding factor (CTCF) binding in rodents
library_construction_protocol: Liver tissue from three male individuals from the Mus species was crosslinked, dounced and lysed as described in Schmidt et al. 2009. (PMID: 19275939) The chromatin was sonicated using a Misonix sonicator 3000 homogenizer with 12 cycles of 30s ON and 60s OFF at a power-output 30 Watt. CTCF was immunoprecipitated using the Merck-Millipore antibody (cat# 07-729) and NGS libraries prepared according to Schmidt et al. 2009 (PMID: 19275939) ligating Illumina's low-plex TruSeq adapters.