title: transcriptome of Glossaulax didyma
design: The adult and healthy G. didyma was obtained from Shandong Weifang Longwei Industrial CO.LTD in Weifang, Shandong Province, China (Table 1). The whole body tissues were sampled immediately and stored in RNAlater and kept at -20 C until RNA extraction. Total RNA of the mixed tissues was extracted using the RNeasy Mini Kit (Qiagen) with a pre-treatment with DNaseI and subsequent elution of the extracted total RNA in nuclease free water, according to the manufacturer's protocol. High-quality mRNA were sequenced using 100 bp paired-end reads on the Illumina HiSeq 4000 platform by Novogene (Beijing, China).