title: Genome sequencing of threespine stickleback from Lake Lobaneshskoye
design: Total genomic DNA was extracted from each individual using Wizard genomic DNA purification kit (Promega). Prior to library preparation, DNA samples of 8 fishes belonging to the population from Lake Lobaneshskoye were pooled in equal proportions. Resulting pooled DNA sample was processed as described in the TruSeq DNA Sample Preparation Guide (Illumina). Library length was estimated using 2100 Bioanalyzer (Agilent) and it is equal to 572. Library was quantified using fluorimetry with Qubit (Invitrogen) and real-time PCR (primers I-qPCR-1.1: AATGATACGGCGACCACCGAGAT and I-qPCR-2.1: CAAGCAGAAGACGGCATACGA) and diluted up to final concentration of 9 pM. Diluted library was clustered on a paired-end flowcell using cBot instrument and sequenced using HiSeq2000 sequencer with TruSeq SBS Kit v3-HS (Illumina), read length 101 from each end.