title: Polymorphism identification for sex locus mapping (low coverage) - MRD30
design: Genomic DNA was extracted by a modified CTAB procedure (Doyle and Doyle 1987).  Extracted DNA was sonicated to a median size of ca. 200 bp.  Samples were prepared for sequencing using standard Illumina protocols.  Sequences associated with chloroplast and mitochondrial genomes were filtered out.