title: Individual Whole Bee no. 1 from Traditional Hive no. 1 sampled August 2016
design: 16S rRNA genes were PCR amplifed with CS1_515F and CS2_926R primers (Walters et al. 2016). The primers contained 5' common sequence (CS) tags and were amplified in a two-stage PCR. Samples were pooled, size selected (425-525 bp) using Blue Pippin Prep. Then loaded onto an Illumina MiniSeq (2x150 bp paired-end reads) to determine distribution of reads to libraries. Samples were re-pooled to balance read distribution, re-size selected, and then sequenced on an Illumina MiSeq v3 flow cell (2x300 bp paired-end reads) with 20% PhiX spike-in.