title: GSM3704052: Degradome_Seq 2; Schmidtea mediterranea; RNA-Seq
library_construction_protocol: Total RNA was extracted from 2 million  FACS-sorted cells  using TriFast reagent  (peqlab) according to manufacturer extraction. Small RNA (< 200 nt) was discarded from total extracted RNA with Zymo-spin IC columns (RNA Clean and Concentrator-5, Zymo Research). Next, rRNA was depleted using custom DNA probes against planarian rRNA (as described in the manuscript). RNA libraries from 2.5 µg total planarian RNA from X1 FACS-sorted cells were constructed as described (Wang et al., 2014).