title: GSM3181671: WT Adult Ileum Villus ATACseq replicate1; Mus musculus; ATAC-seq
library_construction_protocol: Villi from the distal 1/3rd (ileum) of mouse small intestine were isolated by incubating in 5mM EDTA in PBS for 45 minutes at 4°C. Villi were dissociated with 4X TrypLE in DMEM for 45 minutes at 37°C. Cells were counted, lysed and immediately used for transposase reaction. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.