title: GSM3030775: 05Gy_6h_P15_S6; Homo sapiens; OTHER
library_construction_protocol: DNA was isolated from cell pellets using a phenol-free approach (Qiagen Genomic DNA Isolation Kit Mini for Blood and Tissue, Version 04/12). DNA was randomly sheared using the Covaris M220 according to the manufacturers' protocol (Covaris protocol truChIP™ Chromatin Shearing Reagent Kit, PN 010179, Rev C 15 August 2013). Sequencing libraries were prepared with the IDeal Library preparation kit (Diagenode, including Primer Set 1, Version 1 / 11_14, Cat. No. C05010020) and hydroxymethylated DNA was selected using hMeDIP kit (Diagenode, monoclonal mouse antibody, Version 2 09.10, Cat. No. C02010031). 5-hmC monoclonal antibody was used for specific selection of hydroxymethylated DNA and washed using IPure kit (Diagneode, Version 2 I 07.15, Cat. No.C03010015). For library amplification we run 12 PCR-cycles in accordance to the manufacturers protocol. hydroxymethylation-Seq