title: GSM4572495: MEF2D; Homo sapiens; OTHER
library_construction_protocol: Nuclei were extracted by incubating cells on ice for 10 min in Nuclei Extraction buffer (NE: 20 mM HEPES–KOH, pH 7.9; 10 mM KCl; 0.1% Triton X-100; 20% Glycerol; 0.5mM spermidine; 1x complete protease inhibitor [Roche # 11836170001]), collecting by centrifugation (600 g, 3 min, 4ºC), discarding the supernatant, and resuspending at [100 µl / 500K nuclei] sample in NE buffer. CUTANA protocol v1.5.1; optimized version of PMID: 29651053 NEBNext Ultra II DNA Library Prep kit (New England Biolabs #E7645S) Cleavage under targets and Release using nuclease