title: RNA-Seq of mouse lens epithelial cell  alphaTN4-1
design: Total RNA was extracted with Trizol, the mRNA was enriched with Oligo(dT) magnetic beads. Then break the mRNA into short pieces with fragmentation buffer. Use the mRNA fragment as template to synthesis the first cDNA chain with six bases random primers.Then add buffer,dNTPs, RNase H and DNA polymerase to synthesis the second cDNA chain. After purification with QiaQuick PCR kit and elution with buffer, conduct the sequnce end repair and connection, following by selection of fragment size with agarose gel electrophoresis. Finally to PCR amplification. Samples of two biological repeats were sequenced on Illumina NovaSeq 6000. The obtained sequence reads were cleaned and mapped to (GRCm38/mm10).