title: GSM2913199: Nip_3w; Oryza sativa; OTHER
library_construction_protocol: Genomic DNA was extracted using the DNeasy Plant Mini Kit (Qiagen) according to the manufacturer's instructions. Then the magnetic beads (Pacific Biosciences, Cat. 100-265-900) were used to purify DNA, followed by washing steps. Finally, DNA was eluted from beads. The amount and quality of native DNA were examined by gel electrophoresis, Nanodrop (OD 260/280, 1.8-2.0; OD 260/230, 2.0-2.2) and Qubit DNA Assay Kit (Life Technologies).  The 20 kb libraries for SMRT sequencing were constructed according to the standard protocol of SMRT RSII platform. Briefly, DNA was fragmented and purified, then the SMRTbell Template Prep kit 1.0 (Pacific Biosciences, Cat. 100-259-100) was used for the library construction. Size selection was performed using Bluepippin to recover fragments longer than 10 kb. The libraries were then purified using AMPure PB magnetic beads (Pacific Biosciences) and quantified using Qubit Fluorometry before the sequencing step. Sequencing was performed on 75  PacBio RSII cells with P6/C4 chemistry.