title: GSM1550096: H1047R 6hr rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol following standard protocol.  1mL Trizol was added directly to cells, RNA was separated from protein and DNA using 200uL chloroform.  Aqueous layer was removed and total RNA was precipitated using 500uL isopropanol precipitation.    This precipitate was redissolved and precipitated with 250uL DEPC treated water folled by 750uL anhydrous ethanol.  Pellet was redissolved in 200uL DEPC treated water. Residual DNA was eliminated using Ambion Dneasy kits. Nugen Ovation protocol