title: GSM2691930: RIP_anti_igg_6H_2; Homo sapiens; RIP-Seq
library_construction_protocol: The RNAs were extracted using Trizol, treated with TURBO DNase (Ambion) at 37 oC for 30 min, purified using an RNeasy Micro Kit (Qiagen) and quantified with RiboGreen (Invitrogen) RNA samples from Native RIP were used to prepare sequencing libraries with the SMARTer Ultra Low Input RNA Kit for Sequencing v3 (Clontech) according to the manufacturer’s instructions, then sequenced on the Illumina NextSeq500 using 150 bp paired-end reads sequencing.