title: Illumina HiSeq 1500 sequencing; Small RNA and total RNA sequencing of C. elegans with mutations in the hrde-1 and emb-4 genes
design: Small RNA and total RNA sequencing of C. elegans with mutations in the hrde-1 and emb-4 genes
library_construction_protocol: Animals were harvested and washed 3X in M9 buffer. Synchronised animals were grown to young adult stage at 20C on HB101 seeded NGM plates. Settled animals were mixed with Trisure reagent, bead beaten using zirconia beads, and the RNA is extracted using chloroform. For total RNA sequencing, Illumina Ribozero kit is used to remove ribosomal RNA from 1ug of total RNA prior to library preparation. RNA sequencing libraries are prepared using NEB Next Ultra library preparation kit.