title: GSM2354231: HD-HR4; Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA in the heart apex was isolated using Trizol and measured (NanoDrop spectrophotometer ND-1000, ThermoFischer Scientific Inc.). Polyadenylated mRNA fraction was isolated using Poly(A) Beads (NEXTflex). Sequencing libraries were prepared using the Rapid Directional RNA-Seq Kit (NEXTflex)