title: GSM3705241: Bcell_WT_smallLN_rep3; Mus musculus; RNA-Seq
library_construction_protocol: alveolar cells were isolated using anti-CD11c positive selection with MicroBeads, directly lysed in buffer RLT Plus and RNA was isolated; For B cell isolation, CD19+ B cells were isolated from lymph nodes using anti-CD19 positive selection with MicroBeads. Cells were lysed directly in Trizol reagent and total RNA was isolated according to the manufacturers protocol Library preparation and rRNA depletion was performed using the 'TruSeq Stranded Total RNA Library Prep Kit' (Illumina)