title: 16S amplicon-seq from human feces of  congenital heart disease patient 21
design: Hypervariable regions V3 and V4 of the  16S rDNA gene were amplified with forward primer  5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3' and reverse primer  5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3', followed by  indexing with the Nextera XT DNA Index Kit (Illumina).