title: European Anchovy Juvenil_1
design: Poly-A messenger RNAs (mRNAs) were purified from 2 _g total RNA using oligo (dT) attached magnetic beads and chemically sheared into short fragments via fragmentation buffer. Sheared fragments were converted to double-stranded comple- mentary DNA (cDNA) using random hexamer primers and a reverse transcriptase Superscript II (Invitrogen). Second strand cDNA was synthesized using DNase I and RnaseH. Subsequently, end repair and single A-base tailing were performed before Illumina adapters were ligated to the cDNA fragments. After an agarose gel size fractionation step to extract fragments of 200 bp, cDNA libraries were enriched by polymerase chain reaction (PCR) amplification. Following this step, the prepared libraries sequenced using the Illumina HiSeq 2000 plat- form with paired-end 101-bp reads.