title: Sequencing the CA candidate region
design: Each sample was target captured using Nimblegen sequence capture arrays, which involved the addition of primer sequences to allow for PCR after the array annealing and elution step. Each sample then had a multiplex identifier sequence added and was pooled and processed following the standard 454 titanium library preparation protocol. The Nimblegen array sequence capture protocol included the addition of a primer to both ends of each fragment: CTCGAGAATTCTGGATCCTC The MID primers are: MID1: sample 6025 - ACGAGTGCGT MID2: sample 6065 - ACGCTCGACA MID3: sample 6074 - AGACGCACTC