title: whole  genomic sequceing of Trypanosoma evansi: 4V1M clone group
design: An Illumina library with insert size of 270 bp and a PacBio Sequel library with an insert size of 20 Kb were constructed and sequenced. After filtration of low-quality reads, 4.58 and 5.73 Gb clean data were obtained. The short-insert library was used for estimating the genome size by 17 K-mer frequency distribution analysis. Falcon-unzip 0.4.0 (release 1.8.6) was used in the de novo assembly of the T. evansi genome. After assembly, the integrity was evaluated via alignments between Illumina HiSeq-generated sequences from the 270 bp insert-size library and genome sequences from the 20 kb insert-size library. These alignments were then analyzed for single base distribution and GC depth bias.