title: GSM2171783: Jurkat_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: After 24hours, Jurkat cell pellet was collected and total RNA was extracted  using qiazol and purified using the miRNeasy Isolation Kit (Qiagen)  according to the manufacturer's instructions RNA samples were first digested with Dnase I to remove possible DNA contaminants and Poly (A) containing mRNA were then purified from total RNA using Poly (T) oligo attached magnetic beads. The purified Poly (A) mRNA were then fragmented using covaris and first strand cDNA was synthesized using random hexamers. This was followed by second strand cDNA synthesis and purification of the cDNA. The cDNA was then subjected to end-repair and 3' adenylated before adaptors were ligated to the 3'adenylated ends. The products were then purified on TAE-agarose gel and PCR amplification was carried out to enrich the cDNA templates. The library was then validated using Agilent Bio-analyzer and ABI StepOnePlus Real-Time PCR System.