title: GSM3498702: Spleen LSCe from mice transplanted with shCtrl-transduced LCLSK-MA9 (mouse 3), treated with atRA; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using QIAshredder 50 columns (Qiagen) and the RNeasy Mini Kit 50 including DNase treatment (Qiagen) according to the manufacturer's protocol. RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library concentrations were determined with the Qubit Fluorometric Quantitation system and the size distribution was assessed using the Experion Automated Electrophoresis System. Sequencing libraries were pooled, diluted, and sequenced on an Illumina HiSeq 4000 using 50 bp single read chemistry.