title: HiSeq of C. maculatus: whole body
design: We targeted a random subset of the genome by digesting genomic DNA from each beetle with the restriction endonucleases EcoRI and MseI. We then ligated adaptor oligos with internal 8-10 bp barcode sequences to the sticky ends of the digested DNA using T4 DNA ligase. We PCR amplified the restriction fragment library with the Illumina PCR primers and iProof high-fidelity polymerase (Bio-Rad Laboratories Inc., Hercules, CA, USA). DNA libraries were then combined and size-selected using a BluePippin (Sage Science, Beverly, MA, USA); 250-350 bp fragments were retained.