title: Illumina HiSeq 2000 paired end sequencing; Tbrucei_H1_RNAi
design: Tbrucei_H1_RNAi
library_construction_protocol: T. brucei bloodstream form parasites (strain Lister 427, antigenic type MiTat 1.2, clone 221a) [Johnson and Cross, 1979] were cultured at 37 degrees C, 5% CO2, in HMI-11 medium. RNA interference of histone H1 was induced for 4 days in culture. Total RNA was extracted using TRIzol standard protocol. Ribosomal RNA was depleted with RiboMinus kit (Invitrogen). PolyA-containing RNA was purified using Dynabeads oligo-(dT) (Invitrogen) and cDNA was synthesized using random hexamers according to Complete RNA-seq Library System kit (NuGEN) instructions.