title: RNA virome from a soil sample collected near the Lake Limnopolar (01-Feb-2009; Byers Peninsula, Livingston Island, Antarctica)
design: RNA viruses were purified by centrifugation, filtration (0.45 um), sucrose cushion (25%) ultracentrifugation and nuclease treatment. Protected viral RNA genomes were extracted by Trizol followed by a treatment with DNase I RNAse free and then ramdomly amplified by Sequence Independent Single Primer Amplification.