title: GSM3499049: MCF7-ARID1A_estrogen rep 2; Homo sapiens; ChIP-Seq
library_construction_protocol: Nuclear lysates were prepared and antibody-bound chromatin was purified using appropriate antibodies. Antibodies used: ARID1A HPA005456, BRG1 ab215998, SNF5 Bethyl A301-087A, ERα ab3575 and Merck Millipore 06-935 antibody mix, H3K27ac Diagenode C15410196 Premium, HDAC1 Diagenode Premium C15410325, ARID2 Bethyl A302-229A, BRD4 from Cheng Ming Chiang's lab. DNA was prepared for Illumina sequencing on the HiSeq 4000 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.