title: GSM2910023: WT Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from isolated tissue samples using the microRNA Purification Kit (Norgen Biotek Corp., Thorold, ON), following manufacturer's protocol. Following elution, mRNA was further purified using the Qiagen RNeasy Kit (Qiagen, Valencia, CA), according to manufacturer's protocol. Quality of extracted mRNA was assessed using DNA Analysis ScreenTape (Agilent Technologies, Santa Clara, CA) to ensure that it was of sufficient quality for library production. Following validation of extracted mRNA, cDNA libraries were generated using the Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). Following library generation and subsequent quality control assessment, cDNA was sequenced using the Illumina HiSeq2500 platform and single-end reads (1x150).