title: GSM4084733: high metastatic cell line cultured alone, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted with Trizol (Invitrogen) and subsequently cleaned and DNase-treated using RNeasy columns (Qiagen). Libraries for RNA-seq were prepared with Lexogen QuantSeq 3'mRNA-Seq library prep kit at the Wistar Institute Genomics Facility and subjected to a 75 bp single-end sequencing run on NextSeq 500, using Illumina's NextSeq 500 high output sequencing kit following the manufacturer's instructions.