title: The influence of Nicotine on the bacterial community of floral nectar: Sample       iswt
design: I was looking in the difference of bacterial community composition in nectar of regular nicotiana plants vs. transgenic plants (the gene for expressing nicotine was knocked down). These are the wt and the irPMT plants. the nectar was collected in a greenhouse in Max Plank in Jena. We amplified the sequences with a standard illumina protocol using 515F 806R primers. We also sampled nicotiana plants in Israel (called ISWT/TB), those were samples in beer sheva area outdoor. Everything besides this was the same.