title: GSM4013811: MUT rep3 [F]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated as previously described (Crist et al., 2019). Briefly, we used the Thermo Fisher GeneJet RNA Purification kit (Thermo #K0732) and quantified RNA via nanodrop (Thermo) and Qubit (Thermo Q33216).  RNA quality was assessed using the Agilent RNA 6000 nanokit (Agilent 5067-1511) before library preparation. Libraries were constructed using Illumina TruSeq RNA Library Preparation Kit v2 (Illumina RS-122-2001). Quality and quantity of the library were assessed using Agilent DNA 1000 chip (Agilent 5067-1504) and Qubit dsDNA HS Kit (Thermo Q32854), respectively.