title: GSM3573165: EL4_RNA_seq_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq libraries from 300,000 EL4 cells using RNAeasy Kit (Qiagen- 74014) according with manufacturer's instructions and including DNAase digestion. Library were constructed using NEBNext Poly(A) mRNA Magentic Isolation module and NEB Ultra Directional RNA Library Prep Kit for Illumina (NEB). Libraries qualities were assed with Agilent BioAnalyzer 2100 (Agilent) and quantified using KAPA Library Quantification kit (KAPA Biosystems). Single end sequencing (75bp) was performed on a NextSeq 500.