title: GSM2905553: WT_liver_mac_LPS_2h_RNAseq_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted into PBS with 10% BSA and 5mM EDTA and spun down at 3000g for 5 minutes. RNA was purified using the PicoPure RNA isolation kit (ThermoFisher) following the manufacturer's instructions. 100ng of total RNA was used to prepare libraries using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina following the manufacturer recommendations. Library quality and quantity were assessed on a Bionalyser and Qubit respectively.