title: GSM1552263: XL6-2; Drosophila melanogaster; RIP-Seq
library_construction_protocol: Immunoprecipitated RNA was eluted with LDS, and isolated using phenol/chloroform followed by ethanol precipitation Illumina RNA-Seq libraries were constructed by first, reverse-transcribing isolated RNA to cDNA followed by a two-step PCR amplification to produced amplified libraries with ligated adapters for illumina sequencing Each template sequence should be comprised of at least two bases segment of adapter sequence followed by a nine bp segment of barcode, with the remaining sequence coming from a putative crosslink site and the sequence leading up to it.  The nine bp barcode consists of a 4bp segment of known, sample specific, experimental barcode (eb) followed by a 5bp segment of random "molecular barcode" (mb).  The eb allows for multiplexing experiments within a single lane on the sequencer. The mb provides for identifying PCR duplicates, as described under data processing.