title: GSM3179665: Control_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from mice frozen skin tissue using QIAzol lysis reagent (Qiagen, Hilden, Germany) and purified with RNeasy mini kit (Qiagen). Transcriptome libraries were constructed with TruSeq Stranded Total RNA LT Sample Prep Kit (with Ribo-Zero Human/Mouse/Rat, #RS-122-2201). In brief, 300 ng of total RNA from each Sample was used to construct a cDNA library, followed by sequencing on the Illumina Hiseq 2500 with single end 50 bp reads and ~30 millions of reads per sample.