title: GSM1429699: p27 CK- BCR-ABL R6; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq libraries were constructed using the TruSeq protocol (Illumina). Poly(A)+ RNA was isolated using oligo-dT bound to magnetic beads. Poly(A)+ RNA was then chemically fragmented. RNA fragments were converted to double stranded cDNA using random hexamer priming. Fragment ends were treated to remove overhanging nucleotides and then a single “A” was added to the 3’ end of each strand to facilitate ligation. Illumina adaptors with barcode sequences was ligated to the fragments. The resulting library was then amplified using polymerase chain reaction with a limited number of round. Unincorporated nucleotides and adaptor dimers were removed using AMPure XP beads (Agencourt). Libraries were combined for multiplexing and sequenced on a HiSeq 2000 sequencer (Illumina). Base call data was assembled into fastq files using the CASAVA suite (Illumina).