title: GSM3179319: ANC_m20h20-4; Caenorhabditis remanei; RNA-Seq
library_construction_protocol: Approximately 100,000 individuals per replicate were flash frozen in Trizol. Total RNA was extracted using a standard Trizol extraction protocol. Poly-A mRNA was isolated from total RNA using the Dynabeads mRNA purification kit (Ambion). mRNA was sheared to 200-600nt fragments using a buffered zinc solution (RNA fragmentation kit; Ambion). cDNA was synthesized from fragments using Superscript III, and sequencing adaptors with in-line barcodes were added to enable multiplexing of samples.