title: ITS-1 Amplicon  sequencing: sediments Gulf of Mexico
design: The amplicons were  obtained by two-PCR approach. The first PCR was made following a touchdown  PCR to obtain the ITS1 amplicons, using the ITS1F and ITS2 primets with  Illumina adaptor attached. The PCR products were purified. The second PCR was  performed to attach the indexex (i7 and i5) to their respective samples, for  subsequent identification. The PCR products were normalized and pooled.