title: GSM1151695: input DNA; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were lysed in 0.5% SDS, lysates were sonicated and clarified. Chromatin was diluted 5 fold and incubated with beads for pre-clearing. After pre-clearing, chromatin was incubated o/n with anti-GATA6 antibody. DNA samples were provided by the user. Input sample corresponds to a balanced blend of the inputs from the three individual samples. Both 20ng of input pool and 6ng of individual sample DNA, as quantitated by fluorometry, were resolved by electrophoresis and fractions of 50-250bp were taken. 1-3 ng of DNA fraction per sample was processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq DNA Sample Preparation Guide" (part # 15005180 Rev. C). Adapter-ligated libraries were completed by limited-cycle PCR with Illumina PE primers (13 cycles). The resulting purified DNA library was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx with SBS TruSeq v5 reagents by following manufacturer's protocols.