title: RNA-seq of Vaccinium uliginosum: leave transcriptome
design: The sequencing library was constructed as following step. First of all, mRNA was separated from total RNA by using Dynabeads mRNA DIRECT Kit (Invitrogen, Shanghai, China) and then into short fragments under the effect of fragmentation buffer. Second, the double-stranded cDNA was synthesized by short fragments templates, random primers, and SuperScript double-stranded cDNA synthesis kit (Invitrogen, Guangzhou, China). Third, the synthesized double-strand was purified by PCR, followed by two reaction including end-repair and add da-tailing. Finally, Illumina sequencing adaptor was ligated into the cDNA fragment and the sequencing library was finished. After agarose gel electrophoresis, suitable fragments were selected for PCR amplification, and its product was sequenced by the Illumina HiSeq 2000 sequencing platform for raw data