title: Houttuynia cordata Thunb. Transcriptome
design: Beads with Oligo(dT) were used to isolate poly(A) mRNA after total RNA was collected from the samples. Fragmentation buffer was added for interrupting mRNA to short fragments. Taking these short fragments as templates, random hexamer primer was used to synthesize the first-strand cDNA. Then the second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and tailing A. After that, the short fragments were connected with sequencing adapters. And, after the agarose gel electrophoresis, the suitable fragments were selected for the PCR amplification as templates. The cDNA library was sequenced on an Illumina HiSeq2000 sequencing platform.