title: Gossypium hirsutum TM-1
design: One microgram total RNA was used to prepare a partially normalized cDNA library optimized for Roche/454 Titanium sequencing (K. Mockaitis, unpublished; Nguyen et al., 2013).   Sequencing was performed on an XLR70 instrument according to the manufacturer, and reads were trimmed  according to GS FLX System Software of the manufacturer (Roche/454 Sequencing) followed by ESTClean (https://sourceforge.net/projects/estclean/) .  Library preparation, sequencing and data cleaning were performed in the Center for Genomics and Bioinformatics, Indiana University.