title: GSM4576057: AGS cells with SFA treatment; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols.Briefly, the purified total RNA was subjected to rRNA removal, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3 'end plus A, linker, enrichment and other steps to complete the sequencing sample library construct.