title: GSM3704217: AR_2331; Sus scrofa; RNA-Seq
library_construction_protocol: EAT was homogenized and RNA was isolated using RNeasy Universal Midi kit (Qiagen, Valencia, CA). Isolated RNA was treated with Turbo DNase (Ambion, Waltham, MA) to minimize genomic DNA contamination. RNA quality was assessed using the Experion RNA analysis electrophoresis kit (Bio-Rad, Hercules, CA). Only samples with an RNA Quality Indicator (RQI) greater than 7 were sequenced. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit v2 (mRNA purification and fragmentation, cDNA synthesis, end repair, 3' adenylation, adaptor ligation, and DNA enrichment) per the manufacturer's instructions. DNA fragment size was determined using Experion DNA 1K chips (Bio-Rad, Hercules, CA) and library quantification was performed using the KAPA Library Quantification kit (KAPA Biosystems, Wilmington, MA).