title: GSM4085999: Chromatin-associated RNA-seq CPSF73-AID Plus auxin rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Isolated nuclei were lysed into an insoluble chromatin pellet and soluble nucleoplamic supernatant. Chromatin-associated RNA was then purified from the insoluble chromatin pellet using Trizol reagent (see Eaton et al. 2018 for detailed method). Chromatin-associated RNA was depleted of ribosomal RNA using Illumina Ribo-Zero (Illumina #20020597). Library was constructed using Illumina TruSeq® Stranded Total RNA (Illumina #20020597).