title: GSM3330882: GW10-Hy; Homo sapiens; RNA-Seq
library_construction_protocol: Hypothalamic tissues were dissected under dissection microscope and subjected to tissue dissociation. The tissue was dissociated into single-cell suspension using a papain-based dissociation protocol. Single cells were partitioned into nanoliter-scale Gel Bead-In-EMulsions (GEMs) by 10x GemCode™ Technology. The cDNA libraries were generated with Single Cell 3' Reagent Kits, according to the manufacturer's protocol. The library was sequenced with 150bp pair-end reads using an Illumina HiSeq4000. Illumina HiSeq4000