title: GSM3181840: 293T_3'SS library_SF3B1-K700E_RNA-seq_ALL; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq cells were harvested 48 hr of transfection using RNAEasy kit (Quiagen) followed by DNAse digestion (Ambion, Turbo) as per manufacturer's instructions. For protein cells were lysed in 1X RIPA buffer ( 10mM Tris-Cl(pH 8.0), 1mM EDTA, 0.5 mM EGTA, 1% Triton X-100. 0.1% sodium deoxycholate) supplemented wuth protease inhibitor ( Roche). Illumina compatible RNA and DNA  libraries were generated using custom primers specific to mini-gene library using 1X NEB high fidelity master mix.