title: GSM3181680: Cdx2-/- (deleted at E15) Small Intestine ATACseq replicate1; Mus musculus; ATAC-seq
library_construction_protocol: Villi from E18 intestines were isolated by incubation in 5mM EDTA in PBS for 45 minutes at 4°C. Single cells were dissociated with 4X TrypLE in DMEM for 45 minutes at 37°C. Cells were immediately used for chromatin transposition. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.