title: V3-V4 16rRNA sequencing of hospital wastewater
design: The V3-V4 regions of the 16S rRNA gene were amplified using the PRK341F/PRK806R primers modified by addition of Illumina-specific adaptors. The amplicons concentration was measured with the Qubit ®dsDNA HS Assay Kit using the Qubit® Fluorometer and the amplicons were pooled into a normalized library which was diluted to a final 4 nM concentration.