title: HiPlex amplicon sequencing
design: Starting from 40 ng CTAB extracted genomic DNA, the Hi-Plex amplification reactions for 171 amplicons and KAPA PCR-free library preparations were done by Floodlight Genomics LLC (Knoxville, TN, USA). The libraries were sequenced with 2x150 PE on a HiSeq3000 (OMRF, Oklahoma City, OK, USA). Paired-end reads were merged with PEAR (v0.9.8) (Zhang et al., 2014) and adapter sequences were removed.