title: GSM1151148: 0hrs; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from MEFs treated with 20ng/mL TNFa for the indicated times before RNA extraction with Trizol (Invitrogen, 10296-010), followed by RNAeasy kit (Qiagen, 74104) and treated with Turbo DNAse Free Kit (Ambion, AM1907). Poly-A RNA was selected for using the MicroPoly(A) Purist kit (Ambion, AM1919).  200 ng polyA RNA was used for each library.   Paired End Directional library construction was performed for dUTP libraries as described (Levin, Yassour et al. 2010) except libraries were size selected by gel purification after ligation and after PCR amplification.  Libraries were sequenced with an Illumina Genome Analyzer II