title: GSM3764075: 10.NPC-1.25uM.Tcf7.r2.sorted; Mus musculus; ChIP-Seq
library_construction_protocol: Cortex of moue embryonic kidneys were dissociated and nephron progenitor cells (NPCs) were purified with MACS (Brown et al., 2015). NPCs were fixed in AutoMACS running buffer with 1% formaldeyde for 30 min in room temperature. Chromatin was extracted with SimpleChIP® Sonication Cell and Nuclear Lysis Buffers (Cell Signaling Technology #81804) following manufacturer's instruction. Sonicated chromatin was immuno-precipitated with the indicated antibodies. The precipitated DNA was subsequently collected with Qiagen minElute reaction cleanup kit (Qiagen, #28204). Libraries were constructed using ThruPLEX-FD Prep Kit (Clontech, # R400523), and subsequently sequenced by Illumina NextSeq 500.