title: GSM3104682: V-RAS rep2; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were removed, flash frozen on dry ice, and RNA was prepared using Trizol reagent. 1 ug of total RNA was used for construction of sequencing libraries. PolyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments using Illumina's TruSeq Stranded Library Prep Kit. Adapter-ligated library was completed by PCR with Illumina PE primers.