title: Illumina HiSeq 4000 paired end sequencing; Bulk segregant analysis of 3D growth defective mutant in Physcomitrella patens (Vx501).
design: Bulk segregant analysis of 3D growth defective mutant in Physcomitrella patens (Vx501).
library_construction_protocol: Protonemal filaments maintained on ABCD medium for seven days prior to harvest of gDNA using CTAB protocol Genomic DNA fragmented, repaired, A-tailed and adaptor ligated before size selection and amplification