title: pahE-seq of bacteria: Oil contaminated soil
design: Bacteria pahE genes were amplified from the contaminated soil metagenomic DNA. The barcodes were ligated to the reverse end of the amplicons.  PCRs were run in quadruplicate independently and purified PCR products were then mixed together for each sample.pahE primers: pahE1F 5'-TGCGGCGGGTGTNAAYGGNAT-3', pahE1R: 5'- CCTGAGGAATCTCGGACATYTSTGCCCARAA-3'.