title: GSM2905573: WT_unstimulated_GROseq_rep2; Mus musculus; OTHER
library_construction_protocol: 5 million cells were used per each biological replicate. After nuclear run-on reaction the RNA extracted using Trizol (Ambion), treated with Turbo DNAse (Ambion AM1907), fragmented using RNA Fragmentation Reagents (Ambion AM8740), purified by running through P-30 column (Bio-Rad 732-6250), dephosphorylated with PNK (New England Biolabs Y904L) and purified using anti-BrdU beads (SantaCruz sc-214314). GRO-Seq libraries were prepared  as described in (Kaikkonen et al. Remodeling of the enhancer landscape during macrophage activation is coupled to enhancer transcription, Molecular Cell, 51 (2013)).