title: GSM4572393: Patient1_PrimaryTumour; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Sample collection protocol: Tissue specimens of colorectal primary tumor, normal adjacent tissue (NAT) and at least one lymph node metastasis (LNM) were obtained by surgery, fixed in tamponed formaline and paraffin-embedded. DNA extraction was performed using QIAamp DNA FFPE Tissue Kit (Qiagen, Germantown, MD, USA), after manual microdissection. Bisulfite treated DNA was ligated with adaptors by using random probing method. Library amplification using indexed primers and library purification. Purified library DNA was eluted in 12 μl of water.