title: GSM3398718: ChIPseq of paf1+ ade6+, replicate 3, H3K36me3; Schizosaccharomyces pombe; ChIP-Seq
library_construction_protocol: Exponentially growing S. pombe cell culture (50ml YES, OD = 1) was fixed with 1% formaldehyde and lysed. Chromatin was sheared with a Bioruptor (Diagenode). 1% of chromatin was used as input contol. For chromatin immunoprecipitation (ChIP), H3K36me3-specific polyclonal rabbit antibody from abcam (ab9050) was used. For a detailed protocol, see Kowalik et al., 2015. For multiplex ChIP sequencing, samples were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L) and NEBNext Multiplex Oligos for Illumina (E7600S).