title: Diversity of the prokaryote community composition in the sediments of the eutrophic Taihu Lake by T-RFLP and pyrosequencing
design: Geographical and seasonal distribution of prokaryote community were investigated in sediments which were collected from the eutrophic freshwater Taihu Lake in November of 2007 and in February, May and August of 2008, which corresponded to the four stages of algal blooming. Terminal-restriction fragment length polymorphism (T-RFLP) analysis and PCR-based 454 pyrosequencing techniques were used in this study. The results showed that there were similarity and diversity of microbial community structures between the samples. And the effects of environmental parameters on bacterial community were also detected.