title: GSM3030361: ORF5-PK15 2; Sus scrofa; RNA-Seq
library_construction_protocol: Total RNA was isolated from PK15 cells using TRIzol reagent according to the manufacturer's instructions. In order to construct cDNA libraries with the TruSeq mRNA LT library kit (Illumina, USA), 1 g of total RNA was used. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100 nt paired-end sequencing by Illumina HiSeq4000 (Illumina)