title: Transcriptome profiling of resistant and susceptible Cavendish banana roots following inoculation with Fusarium oxysporum f.sp.cubense tropical race 4
design: mRNA was purified from 30 µg of total RNA using Sera-mag Magnetic Oligo (dT) Beads (Illumina) and fragmented into small pieces using divalent cations under elevated temperature. Double-stranded cDNA was then synthesized using the SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, Camarillo, CA) with random hexamer (N6) primers (Illumina).