title: miRNA-seq
design: Total RNA was extracted using Trizol and resolved onto PAGE gels and small RNAs with a size of 18  30 nt were recovered. For construction of sequencing libraries, small RNAs were added with 5 and 3 adaptors, respectively, followed by PCR enrichment. PCR products were resolved onto PAGE gels again and the fraction ranging from 62  75 nt was excised, eluted and analyzed by Bioanalyzer.