title: Diversity assessments from microbes to mammals with eDNA metabarcoding in the Monterey Bay National Marine Sanctuar
design: The protocol is aimed at amplifying the hypervariable region of the mitochondrial DNA 12S rRNA gene in eukaryotes. The primers (MiFish_U_F and MiFish_U_R) used in this protocol were developed by Miya et al., 2015 for metabarcoding environmental DNA (eDNA) from fishes.