title: Complete Genome of WSSV: black tiger shrimp
design: The dead and moribund shrimp (Penaeus monodon) samples were collected from the WSSV prevalent zone of South-West region of Bangladesh (Satkhira) by properly maintained cold chain. Intact WSSV genomic DNA (BAN_SS_304) was extracted from tissue of the Penaeus monodon by Maxwell16 automated nucleic acid extraction system (Promega, Madison, WI, USA) using Maxwell16tissue DNA purification kit (Promega, Madison, WI, USA; Catalog-AS1030) according to manufacturers instruction. The presence of the WSSV DNA was tested by Polymerase chain reaction amplification according to Rout et al 2007. A whole-genome sequencing (WGS) method was executed on Illumina HiSeq TM 2000 sequencer to obtain the complete genome sequence of WSSV by using the DNA isolated from the WSSV-infected shrimp. Covaris M220 ultrasonicator (Covaris, Woburn, MA) was used to shear the extracted DNA into approximately 350 bp fragments. A 350-bp fragment library was constructed using the NEBNext UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturers instructions. The DNA sequencing was performed with Novaseq6000 platform (Illumina) using the NovaSeq 6000 Reagent Kit.