title: 16S WRP effluent, Oct 2013
design: Water was collected from the WRP outlet where the released effluent mixes with stream water of the North Shore Channel, and passed on-site in succession through ~1.6 um pore size glass fiber filter for pre-filtrationand and captured on a 0.22 um pore size polycarbonate membrane filter for collecting free-living microbial component. DNA was extracted using phenol:chloroform based extraction, and used for PCR amplification of 16S rRNA gene with primers 27F/534R. PCR products were submitting for sequencing in paired-end format (300bp each).