title: 16S rRNA of adult female stool microbiota: Sample E22
design: DNA was amplified using primers targeting the V1V2 region of the bacterial 16S rRNA gene, and overhang adaptor sequences were appended to the primer pair for compatibility with the specific Illumina index and sequencing adaptors. Negative controls, not containing template, were amplified for all barcode-primer sets. Final libraries were again purified using AMPure XP reagent (Beckman Coulter), quantified and normalized prior to pooling. The DNA library pool was then denatured with NaOH, diluted with hybridization buffer and heat denatured before loading on the MiSeq reagent cartridge (Illumina) and on the MiSeq instrument (Illumina). The two Illumina sequencing runs (MiSeq1 and MiSeq2) were converted to multiplexed Illumina version 1.9 FASTQ format using CASAVA 1.8.2. 