title: GSM4013614: Patient 1 Plate 2 (P1_P2); Homo sapiens; RNA-Seq
library_construction_protocol: Cells were heat-lysed at 65° C followed by cDNA synthesis using the CEL-Seq2 protocol using robotic liquid handling  platforms Nanodrop II (GC Biotech) and Mosquito (TTP Labtech). After second strand cDNA synthesis, the barcoded material was pooled into libraries of 384 cells  and amplified using in vitro transcription (IVT). Following amplification, the rest of the CEL-seq2 protocol was followed for preparation of the amplified cDNA library, using  TruSeq small RNA primers (Illumina). The DNA library was paired-end sequenced  on an Illumina Nextseq™500, high output, 1x75 bp. After Illumina sequencing, Read 1 was assigned 26 base pairs and was used  for identification of the illumina library barcode and cell barcode. Unique molecular  identifiers (UMI) tags were added to each read. These are molecular tags used in  order to detect and quantify unique mRNA transcripts. More specifically, mRNA  libraries were generated by fragmentation and reverse transcribed to cDNA with tag-  specific primers. R2 was assigned 60 base pairs and used to map to the reference  transcriptome of Hg19.