title: Whole Genome Sequencing for N5 (strobe)
design: For each sample strobe libraries were constructed using Pacific Biosciences commercially available Template Prep Kit, similarly to what has been previously described.  For strobe libraries, abasic sites were engineered into the SMRTbell hairpins to prevent the polymerase from “wrapping” (i.e. going around the adaptor to sequence on the opposite strand).  For each library, a sequencing primer was annealed to each SMRTbell, and the libraries bound to DNA polymerases using 3nM of the respective SMRTbell library and 3X excess DNA polymerase at a concentration of 9nM using Pacific Biosciences’ commercially available DNA/Polymerase Binding Kit 1.0, similarly to what has been previously described (D Rasko, et al. N Engl J Med 365, 709-717 (2011)).