title: Libraries for estimating mutation rates in P. reticulata
design: To estimate the mutation rate family trios were used. We crossed a Quare (laboratory strain, originally from the Quare River in North East Trinidad) female with an EnUlmBL (laboratory strain, presumably originally from Venezuela) male. Restriction-site associated DNA (RAD) libraries of the parents and 5 F1 individuals were prepared using the restriction enzymes PstI and MseI according to (Poland et al. (2012) PLoS ONE 7(2): e32253. doi:10.1371/journal.pone.0032253). We used 8 unique barcodes for each parent and one unique barcode for each F1 individual. The RAD library, with an approximate insert size of 120 – 220 bp, was sequenced single-end on a HiSeq 2000 (Illumina) lane.