title: Whole genome analysis of Pseudomonas aeruginosa N002 suited to remediate crude oil contaminated soil.
design: 5 micro gram of genomic DNA wsa fragmented using Covaris system in the 150-200 bp range. After end repair of the fragmented DNA , Ion Torrent specific adaptors were ligated to the DNA followed by size-selection with the mean size of 180-210 bp. Amplification of the size-selected DNA was carried out. The prepared library was quantified on Agilent Bioanalyzer using High Sensitivity DNA Chip. The procedure was performed as per Ion Fragment Library Kit Manual.