title: GSM1151384: empty vector (EV) day 0 (D0) rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were sorted (D0), collected, washed and gently centrifuged. Cell pellets were lysed in Ambion's miRVana RNA extraction lysis buffer and applied to kit columns. Total RNA was eluted and concentrations were assessed using a nanodrop spectrophotometer. Bioanalysis of total RNA to assess quality and subsequent size fractionation by gel electrophoresis was done at Medomics, Inc. (Azusa, CA). The guidelines provided in the user guide by Applied Biosystems were followed. Total RNA samples were used to generate cDNA libraries. Each cDNA had adapters on both ends giving a total of approximately 42 extra baseparis. The fragments were size selected on gels by excising fragments in the of 55 to 90 bp (target size 60-80 bp). These size-selected cDNAs were then sequenced on the SOLiD 4.0 platform.