title: GSM2934704: 2iL EB d7; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using the RNeasy mini kit (Qiagen). RNA integrity was assessed on a Qubit Fluorometer (ThermoFisher Scientific) and Agilent Bioanalyzer Nano Chips (Agilent Technologies). Depletion of ribosomal RNA was performed on 2-5 μg of total RNA using the Ribo-Zero rRNA Removal Kit (Illumina) and libraries were produced from 10-100ng of ribosomal-depleted RNA using NextFlex Rapid Directional RNA-seq Kit (Bioo Scientific) with 12 cycles of PCR amplification. Libraries were pooled in equimolar quantities and sequenced on the HiSeq4000 platform (Illumina).