title: Illumina HiSeq 2500 sequencing; RNA sequencing analysis for differentially expressed genes in neonatal rat cardiomyocytes following knockdown of transcription factor Vezf1
design: RNA sequencing analysis for differentially expressed genes in neonatal rat cardiomyocytes following knockdown of transcription factor Vezf1
library_construction_protocol: Neonatal rat ventricular cardiomyocytes were treated with either control or Vezf1 siRNA for 72 hours. Cell samples were washed twice with PBS and snap frozen at -80 deg. celcius Vezf1 siRNA Total RNA was collected using EZNA Total RNA kit I (Omega Bio-tek, Norcross, GA)  Nuclease-Free water (Thermo Fisher Scientific, Waltham, MA) was used to dissolve the RNA pellets or eluate the RNA from isolation columns For cDNA synthesis Transcriptor 1st strand cDNA synthesis kit (Roche, Basel, Switzerland) was used according to manufacturer's instructions.