title: 237T
design: Total RNA was isolated from tumor tissue samples using a MagNA Pure Compact RNA Isolation Kit (Roche, Switzerland). The RNA concentration was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA). The RIN was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RIN for all samples studied was no less than 7.0. Libraries were prepared using a NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs, USA) according to the manufacturer's protocol. Libraries were validated prior to sequencing using a quantitative PCR (qPCR).