title: GSM2593256: IVF_fri17LH7E; Homo sapiens; RNA-Seq
library_construction_protocol: Tissue samples were extracted with Pipelle® catheters under sterile conditions from 23-36-year-old  healthy and IVF women in with BMI of 19-29 Kg/m2, rinsed with PBS and processed immediately or frozen at -80 °C. Up to 30 mg of the tissue was homogenized in the presence of QIAzol reagent (Qiagen) and processed using miRNeasy Mini kit (Qiagen), following manufacturer's protocol. miRNA content was decreased using Qiagen's MinElute protocol. Purified RNA quality was assigned with Bioanalyzer (Agilent Technologies).cDNA libraries were generated from ~1 μg of endometrial total RNA using Illumina TruSeq Stranded technology (Illumina), followed by cDNA quality control with Bioanalyzer