title: msh2_2P70
design: Illumina sequencing libraries were constructed by sonicating 1.5 ug of DNA using a bioruptor (Diagenode) until fragment sizes < 2,000 bp were obtained. Following A-tail end-repair, Illumina adaptors were ligated to the fragment ends. Samples were run on a low melting temperature 2% agarose gel and DNA excised and eluted from the 175-225 bp range. PCR amplification was used to enrich fragments to generate final sequencing libraries at a concentration of 10nM.