title: GSM1434983: MCF10A CDH1-/- rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted at 48 h post seeding using quick-RNA Miniprep Kit (Zymo) according to the manufacturer’s protocol. RNA yield and purity were assessed using Qubit (Invitrogen) and the Agilent 2100 Bioanalyser. cDNA library preparation was performed by New Zealand Genomics Limited using Illumina TruSeq RNA preparation version 2.0. Each library had inserts of 200bp and sequence reads were generated from one lane of an Illumina HiSeq™ 2000 run.