title: GSM3104285: BAF170-ChIPSeq 2; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Samples that met the Illumina sample input guidelines were prepared according the NuGEN  Ovation ultralow system V2 Kit with minor modifications. Libraries were size selected for an average size of 350 bp using SPRI-based bead selection. Quality and quantity of the finished libraries were assessed using an Agilent DNA1000 chip and Qubit¨ dsDNA HS Assay Kit, respectively.   Libraries were standardized to 2_M.  Cluster generation was performed using standard Cluster Kits (v3) and the Illumina Cluster Station.  Single 100bp sequencing was performed, using standard SBS chemistry (v3) on an Illumina HiSeq2000 sequencer.  Images were analyzed using the standard Illumina Pipeline, version 1.8.2.