title: Pac-Bio whole genome sequencing of Novosphingobum tardaugens NBRC 16725
design: Highly pure gDNA was isolated using phenol chloroform extraction method and its quality was checked in 1% agarose gel, 30min running at 100V, 0.5ul of DNA loaded.  Shearing method used was g-tube to construct a 10 kb SMRTbell Templates library of 9172 kb in size, which was checked on a Agilent Technologies DNA QC-Bioanalyzer DNA 12000chip. The sequencing reagent used was SMRT Cell 8Pac V3, DNA Polymerase Binding Kit P6, yielding: Mean subread length of 6,067,  N50 of 7,616, a total number of bases of 1,248,591,484 and a number of reads of 205,778. De novo ssembly was performed using RS HGAP Assembly (v3.0) with default options.