title: GSM2850305: G9a.mut.2; Mus musculus; RNA-Seq
library_construction_protocol: cDNA was prepared and amplified as earlier described (Tang et al., 2010). This strategy results in a 3'bias in sequencing. Illumina libraries were prepared as published (Huang et al., 2017). cDNA were fragmented to ~200 bp with Focused-ultrasonicator (Covaris, Woburn, MA) and adaptor ligated libraries were generated using NEBNext II Ultra DNA library Prep Kit for illumina (New England Labs, Ipswich, MA). Single-end 50 bp sequencing was performed with HiSeq1500 (Illumina, San Diego, CA)