title: GSM3399494: ATAC_LIF; Mus musculus; ATAC-seq
library_construction_protocol: 2x105 low passage (< 10) E14 cells were plated per 10 cm culture dish and cultivated for 48 h in regular ES medium containing LIF. 4 h prior to harvest cells were treated with LIF or RA as described in the treatment protocol. 75,000 cells per treatment were subjected to transposition reaction and PCR amplification of accessible regions by Omni-ATAC-seq as described previously by Corces et al. (PMID: 28846090). Sequencing libraries were constructed using Omni-ATAC-seq ) (Corces et al., PMID: 28846090), a modified version of the Nextera DNA Sample prep kit (Illumina, FC-121-1030).