title: Illumina NovaSeq 6000 sequencing; Single cell RNA Seq of cortex cells from adult human ovaries
design: Single cell RNA Seq of cortex cells from adult human ovaries
library_construction_protocol: Whole ovaries were received, medulla was trimmed away using scalpels and remaining cortex was cry-preserved in 1x1x1mm pieces using slow-freezing in Ethylene Glycol. After thawing of tissue, pieces were cut into smaller pieces using scalpels and enzymatically digested (Liberase, Collagenase IV, DNase I) on 37C. After digestion into single cells, cells were either prepared for Dead Cell Removal kit (run1) or FACS (run2). Single droplets containing one cell/droplet was prepared from the cell suspension in 0.04% BSA using 10x chromium controller. Nucleic acid extraction was performed per in chip inside 10x chromium controller. 10x Genomics single cell 3' protocol was followed.