title: RNA-seq of Pseudopestalotiopsis camelliae-sinensis inoculated with PDB and DMSO
design: Mycelial plugs (6 mm diameter) from the edge of 5-day-old colony of the strain GZHS-2017-01 was transferred to PDB and inoculated in shaker with 28C, 180 rpm for 2 d. Mycelia treated with 0.5% DMSO for 12 h were ground into fine powder under the protection of liquid nitrogen. About 100 mg mycelia powder were used for RNA extraction. Total RNAs were extracted using the EASYspin Plus Plant RNA Kit (Aidlab, China). A total amount of 3 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample.