title: GSM2906483: MK5719DEH_TAC; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: RNAs were extracted by acid phenol-chloroform extraction method. Total RNAs were used for synthesis of the cDNA using a Library Preparation kit (DNAFORM, Japan). 5'caps of RNAs are biotinylated after reverse transcription.RNA-cDNA hybirds are captured by streptavidin-conjugated magnetic beads.The cDNAs were released from RNAs and ligated with a barcode sequence linkers. CAGE sequencing were performed using the Illumina NextSeq500.