title: Illumina HiSeq 2000 paired end sequencing; Genome-wide maps of H3K4me1 in Mycobacterium tuberculosis H37Rv-infected macrophages
design: Genome-wide maps of H3K4me1 in Mycobacterium tuberculosis H37Rv-infected macrophages
library_construction_protocol: THP-1 cells (ATCC TIB-202) were cultured in RMPI-1640 with L-glutamine and 25mM HEPES (Wisent) with 10% FBS. H37Rv (ATCC 25618)  were cultured in Middlebrook 7H9 (Difco) with 0.05% Tween-80, 0.1% Glycerol and 10% ADC enrichment (BD Biosciences). The libraries were then prepared using the Trueseq library kit from Illumina and according to the manufacture’s recommendations.