title: PacBio DNA-Seq of K. pneumoniae S01
design: Genomic DNA was extracted from a log-phase culture of K. pneumoniae S01 using QIAGEN Genomic-Tip 100/G and Genomic DNA buffer set as per manufacturers instructions. Nanodrop was employed to assess DNA purity with the criteria 260/280 in 1.8-2.0 and 260/230 in 2.0-2.2. Qubit was used to validate the content of dsDNA (>170 ng/L, >11 g in total amount). Pippin Pulse Electrophoresis was conducted to check the DNA fragment size.  A 15-20 Kb Genomic P6 Library was constructed for PacBio SMRT (single molecule real time sequencing) sequencing (Ramaciotti Centre for Genomics, NSW, Australia)