title: GSM3184526: ZT20_seedling_9; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated from one grinded seedling using the MagMAX-96 Total RNA Isolation kit (Ambion, AM1830), following the manufacturer's instructions. RNA quality and integrity was assessed on the Agilent 2200 TapeStation. Library preparation was performed using the TruSeq Stranded mRNA library preparation kit a (Illumina, RS-122-2101), following manufacturer's instruction, for 1 ug of high integrity total RNA (RIN>8) into which 2 ul of diluted 1:100e ERCC RNA Spike-In Mix (ThermoFisher, cat 4456740) was added. The libraries were sequenced on a NextSeq500 at the Sainsbuty Laboratory, Cambridge University (Cambridge, UK), using paired-end sequencing of 75 bp in length.