title: GSM3330435: TRP47; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were cross-linked using a final concentration of 1% formaldehyde for 10 min. Cells were then pelleted and lysed with lysis buffer and a dounce homogenizer. Lysate was sonicated on ice using Sonics Vibra-Cell (20 cycles, 30 sec at 5 W output, 30 sec rest) to generate chromatin fragments around 300 bp in length. TRP47 was immunoprecipitated using rabbit anti-TRP47 at optimized concentration and pre-immune serum was used as a control. Washing of IP reactions and reversal of crosslinks were performed according to Magna ChIP protocol, and nucleic acid was purified and quantified using a Qubit fluorometer. Preparation of indexed DNA sample libraries involving PCR amplification with adapter ligation was performed by the UTMB Next Generation Sequencing Core using the NEBNext Ultra II DNA Library Kit. Library quality and quantity was evaluated using Agilent Bioanalyzer. Samples were sequenced using an Illumina HiSeq 1500 to generate about 40 million single-end 50 base pair sequence reads per sample.