title: GSM1151773: Axb1_Non_Stimulated; Mus musculus; RNA-Seq
library_construction_protocol: Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. Total RNA (Qiagen RNeasy Plus kit) was isolated from the non-stimulated cells (plated overnight), stimulated and infected cells and the integrity, size, and concentration of RNA checked (Agilent 2100 Bioanalyser). The mRNA was then purified by polyA-tail enrichment (Dynabeads mRNA Purification Kit; Invitrogen), fragmented into 200╨400 base-pair, and reverse transcribed into cDNA before Illumina sequencing adapters were added to each end. Libraries were barcoded, multiplexed into 4 samples per sequencing lane in the Illumina HiSeq 2000