title: 293T_bulk_Cas9
design: Total RNA was extracted from approximately one million HEK293T cell using a miRNeasy Micro kit (QIAGEN).The libraries were constructed following the manufacturer's protocol using NEBNext kits (E7490&E7530),before PCR enrichment, the DNA was digested by Cas9 nuclease with sgRNA mix at 37 degree Celsius for 2h.