title: Genome sequence survey of Paraphysomonas sp. RCC383
design: Illumina compatible Genomic DNA libraries were generated on the Apollo 384 liquid handler using KAPA Biosystems LTP library preparation kit (KK8232). DNA was sheared to approximately 600bp fragments using Covaris M220 ultrasonicator, end repaired and A-tailed as described in the Kapa protocol. Illumina-compatible adapters with unique indexes (IDT #00989130v2) were ligated on each sample individually. The adapter ligated molecules were cleaned using Kapa pure beads (Kapa Biosciences, KK8002), and amplified with Kapas HIFI enzyme (KK2502). Each library was then analyzed for fragment size on an Agilents Tapestation, and quantified by qPCR (KAPA Library Quantification Kit, KK4835) on Thermo Fisher Scientifics Quantstudio 5. Libraries were then multiplexed and sequenced on 2x250 flow cell on the MiSeq platform (Illumina) at the ASUs Genomics Core facility.