title: GSM3573687: 0 h rep3; Chromochloris zofingiensis; RNA-Seq
library_construction_protocol: Total RNA was extracted  using the plant RNA extraction kit (TaKaRa, Japan) and poly-A  mRNA was purified using poly-T oligo-attached magnetic beads. The cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers, which was followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments then had the addition of a single 'A' base and subsequent ligation of the adapter. The products were purified and enriched with PCR amplification. The PCR yield was quantified by Qubit, and samples were pooled together to make a single strand DNA circle (ssDNA circle), which gave the final library.