title: scRNA-seq of d86 H9 ESCs after neuronal directed differentiation
design: Cellular suspensions were loaded on a 10x Genomics Chromium Single Cell instrument to generate single cell GEMs. Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3 Gel Bead and Library Kit. Sequencing libraries were loaded on an Illumina HiSeq4000 with 2  50 paired-end kits using the following read length: 26bp Read1, 8bp I7 Index, and 98bp Read2.