title: Zostera2009_T3_Zm_south_control
design: RNA was pooled from seven different genotypes of the respective experimental condition. Total RNA (ca. ~5 μg per library) was sheared with ultrasound and 3' polyA fragments were purified by oligo(dT) chromatography (3' UTR isolation). First-strand cDNA synthesis was performed using oligo(dT) priming followed by 12 – 15 cycles of PCR (GATC Biotech, Konstanz, Germany; proprietary protocol). Further details can be found in Franssen et al. (2014), Marine Genomics, Genome-wide transcriptomic responses of the seagrasses Zostera marina and Nanozostera noltii under a simulated heatwave confirm functional types, DOI: 10.1016/j.margen.2014.03.004