title: GSM4572434: HK-GFP-hMR_mRNA_Spironolactone; Homo sapiens; RNA-Seq
library_construction_protocol: At the end of the treatment time, cells were washed twice with ice-cold 1X PBS and frozen at -80°C. For each experimental condition, RNA from four 12-well plates were extracted with the NucleoSpin miRNA extraction kit (Macherey-Nagel) following the manifacturer instructions. Each sample consisted on a pool of an equal volume of the four biological replicates. Directional transcriptomic libraries were prepared and sequenced on an Illumina NextSeq 500 sequencer (single read, 75 bases).