title: GSM2856236: vehicle-1; Mus musculus; RNA-Seq
library_construction_protocol: Tissues collected into RNAlater were blotted dry and transferred into Trizol (Thermo Fisher Scientific) in a Lysing Matrix D tube (MP Biomedical) for homogenization.  Supernatant was collected for chloroform phase separation and RNA was precipitated using isopropyl alcohol.  The RNA pellet was then washed with 75% ethanol and redissolved in water. Libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500ng of purified total RNA according to the manufacturer's protocol.