title: GSM4572257: Tcells_PP_index1; Homo sapiens; RNA-Seq
library_construction_protocol: After obtaining the PBMCs for each phase, CD3+CAR+ (CAR-T) and CD3+CAR- (endogenous T) cells were collected by fluorescence-activated cell sorting (FACS, BD FACS Aria II) with anti-Mouse IgG Biotin (Jackson), FITC Streptavidin (Biolegend), and anti-human CD3 APC (clone UCHT1, Biolegend). Single-cell suspensions of all samples were resuspended in DPBS-0.04% BSA at 1x106 cells/mL. ScRNA-seq libraries were generated from the 10X Chromium Single Cell 3` Reagent Kits v2 (PN-120237) according to the manufacturer's protocol (10x Genomics). Libraries were sequenced on the Illumina Hiseq X10 platform according to the manufacturer's instructions (Illumina). Read 1 and Read 2 (paired end) were 150 bp, and the index primer was 8 bp. All samples were sequenced to an average depth of 236 million paired-end reads per sample, with 150 bp on the Read 1 and Read 2.