title: GSM2914997: Organoid 1; Homo sapiens; RNA-Seq
library_construction_protocol: Mouse kidneys were dissected into ice cold PBS then digested over 15 minutes at 37C in Accutase, with manual dissociation via pippetting through a P1000 tip every 5 minutes. Following dissociation, cells were passed through a 30 micron filter and stored on ice in 50% PBS, 50% DMEM with 10% FCS. Human kidney organoids were dissociated via a similar protocol using Trypsin instead of Accutase and stored on ice in APEL media. Chromium Single Cell Library kit V2 Illumina HiSeq HT chemistry- 100bp paired end sequencing