title: GSM3704421: ASCL1 binding region in NCI-H209; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq of H209 cells were performed using 5 μg of anti-TTF-1 antibody (#MS-699-P, Lab Vision Corporation, Fremont, CA, USA) or anti-ASCL1 antibody (#556604, BD Biosciences, Franklin Lakes, NJ, USA) as described previously (Koinuma D et al, Mol cell biol, 2009). The libraries were constructed as described (Murai et al, Cell Discov, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).