title: SRA.total.ox.hi5
design: Hi5 cells were cultured in Express Five serum free medium and then total RNAs were isolated from collected Hi5 cells using mirVana miRNA isolation kit (Ambion). We size selected 18-40nt long small RNAs and followed protocol "Zamore Lab Illumina TrueSeq Small RNA Cloning protocol (April, 2014)" to generate oxidized small RNA library. Oxidized library only contains small RNAs with modification (in this case 2′-O-methylation) at the 3′ ends.