title: GSM3030506: CCTt15; Mus musculus; Bisulfite-Seq
library_construction_protocol: Tissues were lysed in lysis buffer, treated with RNase, and purified with a phenol-chloroform mixture RRBS libraries were prepared from genomic DNA of 3 normal liver tissues and 3 tumor tissues  having a Ha-ras mutation according to the protocol reported by Boyle et al (Genome Biol. 2012, 13:R92. doi: 10.1186/gb-2012-13-10-r92). with some modifications. Briefly, 100 ng of genomic DNA was digested with MspI, subjected to gapfilling  and A-tailing, and ligated with TruSeq adaptors included in the TruSeq DNA Sample Prep  Kit (FC-121-1001, Illumina). After DNA size selection and ligation efficiency were checked,  adaptor-ligated DNA was subjected to bisulfite conversion and amplified by PCR. The PCR  products were cleaned-up using Agencourt AMPure XP beads