title: 16S rRNA sequencing of mus musculus: Colonic contents
design: Total DNA was extracted using the QIA stool extraction kit (Qiagen Inc., Valencia, CA) with the addition of a bead-beating step. Amplicon libraries were constructed that amplified the V3-V4 region of the 16S rRNA gene according to the protocol from Illumina (16S Metagenomic Sequencing Library Preparation). A paired-end sequencing run was performed on the Illumina MiSeq Platform (Illumina Inc. San Diego, CA) using 2 x 300 cycles.