title: GSM3573698: CD6; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol reagent The purified mRNA was fragmented (200bp-300bp) using divalent cations under elevated temperature. These short fragments were copied into the first-strand cDNA using random hexamer primers and reverse transcriptase (Invitrogen, Carlsbad, CA, USA). Second-strand cDNA was synthesized with adding of DNA Polymerase I and RNase H. The products were then enriched by PCR amplification for 15 cycles, separated by Certified Low Range Ultra Agarose (Bio-Rad, USA) gel electrophoresis, and further analyzed for quality on an Agilent 2100 Bioanalyzer (Agilent technologies). The amplicons in the sizes of 300–400 bps were collected and purified.