title: Bonita 3.3
design: DNA was  isolated from sediment samples (0-5 cm) using commercial kit. The 16S rRNA  gene amplicons (V5 to V9 region) was sequenced in 1/8 picotiter plate using  454 GS-FLX Titanium (454 Life Sciences) by Macrogen Inc. (Seoul, Korea). The  primer set used was 787F (5'- ATTAGATACCCNGGTAG - 3') and 1942R (5'-  GNTACCTTGTACGACTT - 3').