title: GSM4085181: RIP-input-Bcell; Homo sapiens; RNA-Seq
library_construction_protocol: Beads-bound immunoprecipitates were then washed six times using cold Wash Buffer with RNase inhibitor, and incubated with protease K in presence of 1% SDS at 55°C for 30 minutes.  RNA and DNA were then extracted from supernatants using phenol:chloroform:isoamyl alcohol and precipitated using ethanol.  Precipitated RNA was digested by DNase I (DNA-free kit, Ambion).  cDNA was synthesized using random hexamer primer by Taqman Reverse Transcription Reagent kit (Applied Biosystems). RNA from anti-SRSF1 immunoprecipitate and input RNA were prepared into RNA-seq libraries using Illumina TruSeq Stranded Total RNA Library Prep kit (Illumina, #20020596).