title: Molecular Analyses of Bacterioplankton Communities Reveal a Hidden Vibrio Reservoir in Natural Seawater near a Coastal Aquaculture Zone
design: The hypervariable V4 region of the 16S rRNA gene was amplified using the primers. PCR amplification was performed as described by Caporaso et al. (2010). Sequencing was performed on an Illumina Miseq platform using a paired-end 250-bp sequencing read run with the Miseq Reagent Kit v3 at the Personal Biotechnology Company (BGI Genomics, Shenzhen, China).