title: GSM2854801: d6_dark_post_2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Male flies were harvested and heads were removed by freezing the flies in liquid nitrogen followed by five rounds of vortexing and freezing. RNA was extracted from isolated GFP-tagged photoreceptor nuclei using previously described method by Ma J. and Weake V.M., J Vis Exp 2014 with minor modifications: nuclei were washed with PBS+300 mM NaCl following affinity purification. Control head homogenate (pre) samples were analyzed for day 1 dark samples. Libraries were prepared using NuGen Ovation Drosophila RNA seq system. rRNA was depleted using InDA-C technology.