title: GSM3704063: CLASH_down 4; Schmidtea mediterranea; RNA-Seq
library_construction_protocol: Worms were dissociated into single cells in cold calcium- and magnesium-free buffer with 1 % BSA (CMFB) as described (Duncan et al., 2015). Next, filtered, pelleted and resuspended in CMFB buffer cells were irradiated at 254 nm as described in the accompanying manuscript. Cross-linked protein-RNA complexes were immunoprecipitated with anti-SMEDWI-3 antibody. Libraries were prepared as described in the manuscript.