title: GSM3573357: JJN3 DC-1-192 1; Homo sapiens; RNA-Seq
library_construction_protocol: Treated and untreated JJN3 samples were resuspended in Trizol reagent (Thermo Fisher) and RNA was extracted using an RNeasy mini-prep kit (Qiagen). Total RNA quality and quantity was assessed using an Agilent 2100 Bioanalyser and an RNA Nano 6000 kit (Agilent Technologies). Approximately 100-900ng of Total RNA with an RNA integrity number (RIN) >8 was depleted of ribosomal RNA and used for RNA-seq. RNA libraries were prepared for sequencing using standard Illumina protocols Following validation, the libraries were normalised to 4nM, pooled together and clustered on the cBot2 following the manufacturer's recommendations