title: GSM3330506: WT_t24_rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: seedlings were harvested and flash frozen, RNA for library preparation has been extracted using the RNeasy Plant RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's protocol poly(A)-RNA was isolated using the NEBnext poly(A) mRNA Magnetic Isolation Module (NEB #7490), this was followed by purification of 5-EU labeled RNA with the Click-iT™ Nascent RNA Capture Kit (Thermo Scientific).  A known concentration of a EU containing  RNA was used as spike-in for normalization. Libraries were prepared using ScriptSeq™ v2 RNA-Seq Library Preparation Kit (epicentre). For library preparation mRNA was used as input starting at the fragmentation step in the protocol (step number 5).