title: RNA_Seq of axenically and conventionally raised mosquitoes
design: RNA was extracted using the mirVana miRNA Isolation kit according to the manufacturers instructions (Ambion). Total RNA was quantified using the BR RNA assay on the Qubit, and 75-bp paired-end libraries constructed RNA-Seq unstranded Libraries construction and sequencing were performed at the Next Generation Sequencing Core at the University of Texas Medical Branch. 75 bp paired-end libraries using the RNA-seq non-stranded