title: GSM1142338: Spombe_Exp2 paired-end; Schizosaccharomyces pombe; OTHER
library_construction_protocol: DNA was purified by phenol-chloroform extraction and subsequent ethanol precipitation. The sample was RNased (1mg/ml final) overnight at 42oC and run on a 2% agarose gel.  The shortest DNA band, corresponding to the center-to-center distance of two nucleosome neighbors, was cut out of gel and the DNA was extracted.  The ends of the chemically mapped DNA were blunted using first NEB’s Klenow Polymerase large fragment (cat # M0210L) then processed further using Lucigen’s DNATerminator End Repair Kit (cat # 40035-2). ABI SOLiD adapters were ligated to the blunted and phosphorylated DNA ends. Nick translation and PCR amplification were completed following ABI’s SOLiD Fragment Preparation protocol. All samples were sequenced with ABI’s SOLiD next generation sequencing system.