title: GSM1429420: MCF10A_10E2_MYC-ERVD_control_ChIP-Seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde. After a hypotonic lysis step, nuclei were resuspended in sonication buffer containing 0.1% SDS and 0.1% Na-deoxycholate and sonication was performed in a Branson sonifier. Anti-ER-a antibody (HC20, Santa Cruz) was coupled to Protein A-Dynabeads, 10E2 mouse anti-MIZ1 antibody was coupled to Protein G-Dynabeads (Invitrogen). Bound chromatin was eluted with 1% SDS and after decrosslinking DNA was purified with phenol-chloroform. ChIP DNA was end-repaired, A-tailed and ligated to Illumina adaptors using NEBNext ChIP-Seq Sample Preparation Kit. After size selection on an agarose gel and purification with a Qiagen gel extraction kit, PCR-amplification was performed. Library quality was tested with an Experion DNA Chip (BioRad) and quantified using a picogreen assay.