title: Tara Pacific Symbiodinium ITS2 primer
design: All PCRs were conducted in triplicate before being pooled for sequencing. Pooled PCR products were purified using 0.8 x AMPure XP beads (Beckmann Coulter Genomics, USA), then aliquots of purified amplicons were run on an Agilent Bioanalyzer (Agilent technologies, USA) using the DNA High Sensitivity LabChip kit to verify lengths and were quantified using a Qubit Fluorometer (ThermoFisher Scientific, USA). One hundred ng aliquots of the amplicons were directly end-repaired, A-tailed, and ligated to Illumina adapters (Illumina, USA) on a Biomek FX Laboratory Automation Workstation (Beckman Coulter, USA). Then, library amplification was performed using the KAPA Hifi HotStart NGS kit (KAPA Biosystems, USA). After library profile analysis by LabChip GX (Perkin Elmer, USA) and qPCR quantification using PicoGreen (ThermoFisher Scientific, USA) on 96-well plates, each library was sequenced using 250 bp read length chemistry in a paired-end flow cell on a MiSeq instrument (Illumina, USA).