title: GSM3181721: Cdx2-/- (deleted at E15) Small Intestine RNA replicate2; Mus musculus; RNA-Seq
library_construction_protocol: Villi from E18 intestines were isolated by incubation in 5mM EDTA in PBS for 45 minutes at 4°C. Cells were dissociated with 4X TrypLE in DMEM for 45 minutes at 37°C. Cells were immediately used for RNA isolation. Total RNA from cells collected in TRIzol reagent (Thermo Fisher Sci.) was extracted with the RNeasy kit (Qiagen) and Dnase treatment. The SMARTer-Seq v4 Ultra Low Input RNA Kit (Clontech) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.