title: GSM2691926: Old_3; Homo sapiens; RNA-Seq
library_construction_protocol: Stimulated cells were washed once with PBS and were then lysed in RLT buffer (Qiagen) and this extract was stored at -80C. RNA was isolated from the lysate using the RNeasy Kit (Qiagen) according to standard manufacturer's protocols. Library construction was completed by Hudson Alpha using a protocol for low input RNA-seq. Details from their website: "The GSL uses the Ovation RNA-Seq System V2 kit (Nugen) for initial RNA amplification which provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications. Our low input RNA-seq service requires 1ng-10ng of total RNA with a RIN score of 7.0 or higher. It allows amplification of both mRNA and non-polyadenylated transcripts. After purification, the cDNA is fragmented to the appropriate size and further library prep steps are followed to prepare sequencing library. There is no directional module available for this RNA-seq service."