title: Bacterial community composition of permaforst thaw ponds in subarctic Quebec: Sample KWK_1_Large_Bottom
design: DNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen). V6-V8 regions of bacterial 16S rRNA converted to cDNA were PCR amplified using forward primer 969 (5'- ACGCGHNRAACCTTACC-3') and individually bar-coded reverse primers 1406 (5'- ACGGGCRGTGWGTRCAA-3'). The resulting 22 Biosamples were sequenced using Roche/454 GS FLX technology.