title: GSM1428949: CvixLer_endosperm_3_mRNASeq; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Seeds from crosses between multiple individuals were hand-dissected and total embryo or endosperm RNA isolated using the Ambion RNaqueous Kit with Plant RNA Isolation Aid or the Ambion RNAqueous Micro Kit. Libraries were constructed essentially using the Illumina mRNA-seq protocol. 0.6-1 micrograms of total RNA were poly-A selected twice, fragmented, and converted into double-stranded cDNA. The cDNA was end-repaired, adenylated, and adapters ligated. Libraries were PCR amplified for 12 cycles using TruSeq primers.