title: GSM1553171: central_Rep1; Alternanthera philoxeroides; RNA-Seq
library_construction_protocol: tissue collection: top 2-3 leaves were excised between 10:00 am-3:00 pm and immediately frozen in liquid nitrogen for future RNA extraction and sequencing library construction. RNA extraction was performed by grinding approximately 0.1 grams of frozen tissue in liquid nitrogen to a fine powder, and adding 1 ml Trizol reagent (Ambion/Life Technologies). Chloroform:Isoamyl (24:1) was added to the Trizol/tissue mixture, centrifuged, and RNA was extracted from the resulting supernatant using an RNeasy Plant Mini kit (Qiagen). Quality control was assessed using a nanodrop bioanalyzer on the RNA setting. Sequencing libraries were generated using NEBNext®Ultra™ RNA Library Prep Kit for Illumina®(NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.