title: GSM1547689: P10.5 Morc1 het bisuflite (4 replicates); Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA for bisulfite sequencing was extracted using the QiaAMP DNA Micro kit (Qiagen) and quantified using the Qubit dsDNA High Sensitivity Kit (Life Technologies). Libraries were prepared using the Ovation Ultralow Methyl-Seq Library System (Nugen). 5ng – 25ng DNA was used as starting material. Matched mutant and control samples always contained identical quantities of DNA. Unmethylated Lambda phage DNA (NEB) was spiked in at 0.5% input DNA quantity to determine conversion efficiency, which was consistently >98%. Each library was prepared using DNA from one individual mouse.