title: GSM4142218: T12.WGBS.AD; Trichinella patagoniensis; Bisulfite-Seq
library_construction_protocol: DNA was extracted using the phenol chloroform extraction method Genomic DNA extracted from three life stages of all Trichinella species were selected for libraries construction of WGBS. Briefly, approx. two to five micrograms of gDNA were first fragmented by Covaris to an average size of ~200-300 bp. End-repair, A-tailing and ligation were conducted as the Illumina PE genomic DNA sample prep kit protocol, except that ligation was performed using methylated cytosine PE adapters provided by Illumina previously. Ligation products were purified and bisulfited converted using the EZ DNA Methylation-Gold KitTM (ZYMO) according to the manufacturer's instructions. The libraries were sequenced using Illumina HiSeq 2,500 platform.