title: mESC_strand_sc5
design: A single cell was lysed with 100 ng of the carrier RNA mixture. The                 sequencing library was constructed following the manufacturer's protocol using a                 NEBNext kit (E7420). After adaptor ligation, the cDNA fragments from the RNA carrier                 were removed by Not I digestion. After Not I digestion, the library DNA was purified                 for USER digestion and PCR amplification.