title: Amplicon seequencing of Arabidopsis thaliana: wild-type
design: T1 seeds were sown on GM media containing cefotaxime and either phosphinotricin or gentamycin, for two weeks. For each construct, 100 T1 plants were pooled and used for DNA extraction. Therefore, primers were designed with a 6 bp tag sequence (Table S2) to amplify inversion and deletion junctions with a length of 370 - 400 bp.