title: RNA-Seq of PDX tumor engrafted in mammary fat pad: Sample RNA-PIM001-mouse1-MFP
design: "Barcoded, Illumina compatible stranded total RNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). Briefly 250 ng of DNase I treated total RNA was depleted of cytoplasmic and mitochondrial ribosomal RNA (rRNA) using Ribo-Zero Gold (Illumina). After purification, the RNA was fragmented using divalent cations and double stranded cDNA was synthesized using random primers. The ends of the resulting double stranded cDNA fragments were repaired, 5'-phosphorylated, 3'-A tailed and Illumina-specific indexed adapters were ligated. The products were purified and enriched with 12 cycles of PCR to create the final cDNA library. Library pools were quantified by qPCR and sequenced in two lanes of the Illumina HiSeq4000 sequencer using the 75 bp paired end format."