title: PARE-seq of oryza sativa japonica group: seedling shoot raw extracts
design: Total RNAs were extracted from raw extracts of rice (Oryza sative japonica group, cv. Nipponbare) seedling shoots with TRI Reagent (Molecular Research Center, USA) and used for PARE (parallel analysis of RNA end) library construction according to the method describe by Zhai et al. [Zhai J, Arikit S, Simon SA, Kingham BF, Meyers BC. Rapid construction of parallel analysis of RNA end (PARE) libraries for Illumina sequencing. Methods. 2014;67:8490.].