title: RNA-Seq of Nelumbo nucifera : stamen
design: Total RNA was isolated using a RNA reagent (OminiPlant RNA Kit, CWBIO, China), and  Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. The library preparations were sequenced on an Illumina Hiseq platform according to manufacturer's instructions.