title: GSM1552038: Grass_222; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA was extracted individually (two animals per group) using Trizol (Invitrogen, Carlsbad, CA, USA) followed by DNase digestion and Qiagen RNeasy column purification (Invitrogen, Carlsbad, CA, USA). The RNA sample was dissolved in RNAse-free H2O; the integrity and quality of RNA were then checked by a NanoDrop 1000 spectrophotometer and by resolution on a 1.5% non-denaturing agarose gel Each library was identified by adding 6-bp adaptors and sequenced at 50 bp/sequence read using an Illumina HiSeq 2000 sequencer