title: RNA-Seq of Dichapetalum cymosum (T:60 post-wounding)
design: cDNA library construction and Illumina sequencing were performed at the Agricultural Research Council (Pretoria, South Africa). Ribosomal RNA was depleted using the RiboZero Plant rRNA Removal Kit (Epicentre). Thereafter, cDNA libraries for each time point (in triplicate) were created individually using the TruSeq Stranded mRNA Library Preparation Kit (Illumina). Paired-end sequencing was performed for each of the time-point samples and their respective replicates on the HiSeq 2500 (Illumina) platform using the HiSeq Reagent Kit v4 (Illumina).