title: GSM3330226: FcgRIIB_neg_2; Mus musculus; RNA-Seq
library_construction_protocol: On day 14 post grafting, 1000 cells were FACS sorted into RLT+1%Bme using the indicated markers. Total RNA was purified using the Quick-RNA MicroPrep Kit (Zymo Research). All purified RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara). Final libraries were constructed using 200 pg of cDNA as input for the NexteraXT kit with the NexteraXT indexing primers (Illumina).