title: GSM1429883: 2cell_replicate1; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA were isolated from individual oocytes/embryos using TRIzol (Invitrogen, Grand Island, NY) and co-precipitated the RNA with linear acrylamide (Ambion). RNA was then amplified twice using the TargetAmp 2-round aminoallyl-aRNA amplification kit 1.0 (Epicentre, Madison, WI) according to the manufacturer’s instructions. 500 ng of amplified RNA (aRNA) from individual oocytes/embryos were used to construct the sequencing library following the manufacturer’s instructions by SOLiD™ Total RNA-Seq Kit (Life Technologies, Grand Island, NY).