title: GSM1148891: ni rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was harvested using RNeasy Mini Kit (Qiagen) followed by mRNA extraction using Dynal mRNA Direct kit. mRNA was then fragmented and reverse transcribed to double stranded cDNA. Sequencing was performed in a single lane with Illumina HiSeq 2000 sequencing instruments at 1x50 single read run. RNA libraries were prepared for sequencing using standard Illumina protocols