title: GSM4086619: CD11b Dendritic Cells WT1; Mus musculus; RNA-Seq
library_construction_protocol: Cells samples were extensively washed in PBS, resuspended in RLT lysis buffer, snapfrozen and kept at -80°C.  RNA extraction and residual DNA removal upon DNase digestion was performed with the RNeasy mini kit (Qiagen) according to the manufacturer's instructions. 7 ng of total RNA, after quality check with Agilent Bioanalyser 2100 system, was made into sequencing libraries using Takara's the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian, following manufacturer's instruction,