title: GSM3498600: HESC_Input; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody Bethyl A300-346A. ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the  3'-ends. Illumina genomic adapters were ligated and the sample was  size-fractionated (200-300 bp) on a 2% agarose gel. After a final PCR  amplification step (18 cycles), the resulting DNA libraries were  quantified and sequenced on HiSeq 2000.