title: GSM2692633: RDsh_Dox ATAC-seq; Homo sapiens; ATAC-seq
library_construction_protocol: 75.000 cells were harvested and treated with transposase Tn5 (Nextera DNA Library Preparation Kit, Illumina). DNA was purified using MinElute PCR Purification Kit (Qiagen). All samples were then amplified by PCR using NEBNextHigh-Fidelity 2x PCR Master Mix (New Englands Labs.) and primers containing a barcode to generate the libraries. DNA was again purified using MinElute PCR Purification Kit and samples were sequenced using Illumina HiSeq 2500 system.