title: GSM3863341: BM_M6_pos_S5; Mus musculus; RNA-Seq
library_construction_protocol: mRNA was isolated using RNeasy Plus kits (Qiagen) and sequencing was performed on an Illumina NextSeq 500 (paired-end sequencing 2x75bp) instrument. Briefly, the quality of the total RNA was assessed using the Agilent 2100 Bioanalyzer (all samples had RIN values of >9.9) followed by two rounds of poly(A) selection and conversion to cDNA. TruSeq library generation kits were constructed by random fragmentation of the polyA mRNA, followed by cDNA production using random primers (Agilent Technologies). The ends of the cDNA were repaired, A-tailed, and adaptors were ligated for flow cell attachment, sequencing, and indexing as per the manufacturer's instructions (Agilent Technologies). cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems) prior to sequencing.