title: Sequel sequencing of SAMD00203090
library_construction_protocol: "The 16S rRNA genes were amplified using KOD DNA polymerase and the primers: 8F (AGA GTT TGA TYM TGG CTC AG) with adaptor and tag sequences and 1492R (GGY TAC CTT GTT ACG ACT T). Sequencing was performed using a Sequel Sequencing kit 2.1. Circular consensus reads were obtained from raw sequence reads using SMRT Link software, and were quality-filtered by R prior to processing by the DADA2."