title: GSM2356640: 72hpa_rep2; Xenopus tropicalis; RNA-Seq
library_construction_protocol: Tadpole tails were amputated and immediately placed in lysis buffer (1% SDS, 0.100M NaCl, 0.010M EDTA, 0.20M Tris ph 7.6, Proteinase K) followed by ethanol precipitation to extract RNA. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols