title: GSM3574729: DYT-N1_RNA&lncRNA&circRNA-Seq; Homo sapiens; RNA-Seq
library_construction_protocol: The whole RNA of HCC and their adjacent paired tissues was harvested using Trizol reagent.Total RNA of the sample was subjected to test the RNA integrity using agarose gel electrophoresis, then quantified and examined by NanoDrop ND-1000 1-2 ug of total RNA was selected for each sample for sequencing library construction. The total RNA was processed by NEBNext® Poly(A) mRNA Magnetic Isolation Module (or rRNA removal by RiboZero Magnetic Gold Kit) to enrich mRNA, and the product RNA was used for construction Library, via KAPA Stranded RNA-Seq Library Prep Kit (Illumina). Sequencing libraries, denatured by 0.1 M NaOH to generate single-stranded DNA, as amplified in situ Illumina cBot (TruSeq SR Cluster Kit v3-cBot-HS (# GD-401-3001, Illumina)). The ends of the generated fragments was used to run 150 Cycles by the Illumina HiSeq 4000 Sequencer. RNA libraries were prepared for sequencing using standard Illumina Hiseq protocols.