title: Illumina HiSeq 2000 sequencing; RNA-seq of the whole retina of rat intravitreally injected with aldosterone
design: RNA-seq of the whole retina of rat intravitreally injected with aldosterone
library_construction_protocol: Male 8-week old Lewis rats (3 control rats and 3 aldosterone-injected rats) were injected intravitreously with aldosterone (20nM final concentration in the vitreous) and sacrificed after 24 hours. Control rats received vehicle injection. The whole retinas were isolated for RNA-sequencing.  Total RNA was extracted using the RNeasyMini Kit (Qiagen) including DNase I treatment according to the manufacturer instructions. Nucleic acid library construction was performed using the TruSeq RNA Library Prep kit v2 (Illumina) following the Illumina kit protocol.