title: MDS assay of tissues in mice from cohort 3
design: Genomic DNA was incubated with restriction enzyme cutting near the genomic region of interest. Digested genomic DNA was column purified. The barcode and adaptor were added to the target DNA by incubating purified DNA with the appropriate barcode primer for one cycle of PCR. Subsequently, exonuclease I was added to remove unused barcoded primers. Processed DNA were column purified. Samples were then linear amplified with forward adaptor primer. Exon-specific reverse primer and reverse-adaptor primer were then added and 20 cycles of exponential amplification were performed. The final library was size selected and purified with Ampure XP beads.