title: Illumina HiSeq 4000 paired end sequencing; Comprehensive bulk RNAseq analysis on whole kidneys during regeneration and fibrosis development reveals sustained dysregulation of immune system related pathways during kidney fibrosis
design: Comprehensive bulk RNAseq analysis on whole kidneys during regeneration and fibrosis development reveals sustained dysregulation of immune system related pathways during kidney fibrosis
library_construction_protocol: For whole kidney bulk RNAseq and qPCR analysis, whole kidneys were weighted and preserved in RNA-later (Qiagen) solution for further processing.  RNA was extracted using RNeasy micro kit (Qiagen), according to manufacturer's instructions. Quality and quantity of RNA was tested with the Agilent 2100 Bioanalyzer using the RNA 6000 Pico Chip and reagents (Agilent).  RNA-seq libraries were prepared with Illumina TruSeq Stranded total RNA Library Prep kit with RiboZero Gold according to manufacturer's instructions.