title: GSM3861330: 63_75Y_P2; Homo sapiens; RNA-Seq
library_construction_protocol: Cell suspensions were centrifuged at 600g, media was removed, and cell pellets were collected for RNA isolation. RNA from each sample was isolated using the Qiagen RNeasy® kit following the manufacturers procedures. RNA quality and quantity of each sample were analysed with the 2200 Tapestation (Agilent). Samples with high RNA integrity number (RIN) of 9-10 and at least 5µg of total RNA collected were used further analysis For each sample, a total of 5ug of RNA was prepared into sequencing libraries using the TruSeq Stranded mRNA Library Prep Kit (Illumina) and each library was sequenced to the depth of 4-7 × 107 reads/sample with the Illumina NextSeq500 75bp sequencing at the Centre for Health Genomics and Informatics at the University of Calgary.