title: GSM1551772: P1 Sorted corticothalamic projection neurons replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was recovered from FACS-purified cells using the RecoverAll Total Nucleic Acid Isolation Kit (Ambion) according to manufacturer’s instructions except proteinase K digestion was performed at 50˚C for 3 hours. RNA concentration was quantified with Nanodrop 1000 and quality was determined with an Agilent 2100 Bioanalyzer. RNA Integrity Numbers (RIN) for all samples were between between 6.3 and 9.  Typical yield was between 1 and 2 picograms RNA per sorted event, requiring approximately 100,000 cells to yield 200 ng RNA for library input. Purified RNA served as input for the standard Illumina RNA-seq library preparation with poly(A) selection.