title: GSM4086616: DA_S2; Rattus norvegicus; RNA-Seq
library_construction_protocol: Neurons were treated with described stimuli for 1hr, washed, and harvested in lysis buffer prior to isolation of nuclei via centrifugation and FACS sorting. FACS-sorted nuclei were subject to 10X GEM generation and barcoding, GEM-RT cleanup and cDNA amplication, and library construction using a Chromium Single Cell 3ʹ Reagent Kit (v3 chemistry). Libraries were quantified and underwent sequencing (~500M total 75bp paired-end reads) on an Illumina sequencing platform (NextSeq 500).