title: GSM2850185: granulosa cells_CCs_C2; Homo sapiens; miRNA-Seq
library_construction_protocol: RNA from PrGC was purified using the miRNeasy micro kit (QIAGEN, Germany) according to the manufacturer's instructions Small RNA libraries were generated from 200 ng total RNA using NEXTflex Small RNA Sequencing Kit v3 for Illumina Platforms (Bio Scientific, Austin, TX, USA). An extra purification of the libraries was performed with the automated agarose gel separation system Labchip XT (PerkinElmer, Waltham, MA, USA). The obtained cDNA fragment libraries were sequenced on Illumina Nextseq500 (Illumina, San Diego, CA) using default parameters (single read 1x50bp). Briefly, 3′- and 5′-adaptor sequences were removed using the CLC Genomics Workbench (CLC Bio, Cambridge, MA). RNA reads were analyzed with miRDeep version 2.0 (Friedländer et al., 2008) Centre and annotated against miRBASE version 21 (http://www.mirbase.org) allowing one mismatch