title: Elucidation of insertion elements encoded on plasmids and in vitro construction of shuttle vectors from the toxic cyanobacterium Planktothrix
design: 1) For each sequencing run 5 microgram of isolated HMW DNA were used 2) Fragmentation of DNA using nebulization 3) Ligation of linkers carrying PCR and sequencing primers 4) Selection of A/B fragments using avidin-biotin purification 5) Isolation of sstDNA carrying A and B linker sequences 6) Emulsion-based clonal amplification 7) 454 sequencing using the 70x75 format Picotiter plate