title: GSM3330492: WT_all_rep1 [NEU]; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: seedlings were harvested and flash frozen, RNA for library preparation has been extracted using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's protocol. Ribosomal RNA was removed with the Ribo-Zero rRNA Removal Kit (Plant) of Epicentre (Illumina). A subsample was taken to sequence the steady state level of gene transcription. 5-EU labeled RNA was purified from the remaining ribo-zero treated RNA with the Click-iT™ Nascent RNA Capture Kit (Thermo Scientific). For library preparation mRNA was used as input starting at the fragmentation step in the protocol (step number 5).