title: GSM4085013: ID12; Bos taurus; RNA-Seq
library_construction_protocol: A small portion of testicular parenchyma (~75 mg) from each bull (n=24) was homogenised and total RNA extracted. Trizol reagent-mediated extraction was performed as per manufacturer's instructions. For each sample (n=24) total RNA (1 µg) was used to create mRNA libraries using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols