title: Paired HC and LC antibody sequencing: Subject3 Timepoint2
design: CD19+CD3-IgD-CD14-CD20-CD27+CD38++ plasmablasts were single-cell sorted (BD FACSAria) into 96-well plates. Well-specific oligonucleotide barcodes (TruGrade Oligos; IDT) were added to cDNA by template switching using Maxima Reverse Transcriptase (ThermoFisher) with an oligodT primer. Wells within each plate were pooled, and HC and LC genes were amplified from pooled cDNA using gene-specific PCR primers. Plate-specific index sequences were added during library preparation, and paired-end sequencing (2X330) performed using Illumina MiSeq.