title: GSM3763548: X721_05_H; Mus musculus; RNA-Seq
library_construction_protocol: Primary keratinocytes were isolated and processed for FACS. After sorting, cells were pelleted by centrifugation and re-suspended in Trizol (Invitrogen) followed by chloroform extraction. The aqueous phase was applied to RNeasy minElute column (Qiagen) and the RNA was purified according to manufacturer instructions. 10 ng of purified RNA were processed with Nugen Ovation RNA-Seq System (NuGen) for mouse, to make strand specific RNA-Seq libraries.