title: GSM2928615: P3_crypt_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Medial small intestines were flushed with cold HBSS, and epithelium was isolated by incubating in EDTA for 20 min.  Cells were FACS sorted based on Epcam and CD44 staining, and cell pellet was frozen at -80 until RNA extraction. Total RNA was extracted and DNAseI treated using Qiagen RNeasy Mini kit according to the manufacturer's protocol. Quality control was evaluated by Qubit and 2100 Bioanalyzer. poly(A)-mRNA libraries were constructed using the KAPA stranded mRNA-seq kit.