title: DNA of Hordeum vulgare subsp. vulgare cultivar Balga: first leaves of shoots
design: DNA was digested with Dnase I. To obtain the residual DNA fragments                 the digest was incubated with Proteinase K and SDS and deproteinized with                 chloroform. Remnants of DNA were precipitated with ethanol. DNA fragments were                 obtained from coleoptiles, first leaves and roots, fractions were called Cd, FLd and                 Rd respectively.