title: GSM3863098: 17-K; Rattus norvegicus; Bisulfite-Seq
library_construction_protocol: Perform the bisulfite conversion and subsequent clean-up using the QIAGEN EpiTect (or an equivalent) kit. Follow the manufacturer's protocol designated for DNA extracted from FFPE tissues for all samples, whether they are from FFPE material or not. Elute the DNA from the column with 22 μl of preheated (65 °C) EB buffer (from the EpiTect kit). A total of 40 libraries were generated according to the previously described protocols by Gu et al. (Nature Protocols volume 6, pages 468–481 (2011)). The libraries underwent cluster generation using TruSeq PE Cluster Kit v4-cBot-HS and 100 cycles of paired-end sequencing using TruSeq SBS Kit v4-HS (Illumina). 13 or 14 libraries were multiplexed in one lane and sequenced on an Illumina HiSeq 2500 sequencer.