title: GSM1547707: RNA extracted from leaves of plants; Cicer arietinum; ncRNA-Seq
library_construction_protocol: Size fractionated small RNA from total RNA extracts of Cicer arietinum leaves and from Nicotiana benthamiana infected by Cymbidium ringspot virus were mixed in a ratio of 1000 to 1 in amount, respectively.  Total RNA was obtained using TriReagent (Sigma) following manual indications and fractionation was done running total RNAthrough a high resolution polyacrilamide gel electrophoresis. RNA 20nt to 30 nt in size was eluted and used for librariy generation. The RNA was  ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Illumina high throughput pyrosequencing. The kit used is TrueSeq Small RNA kit Please see www.illumina.com for details of the sequencing technology.