title: GSM3181712: WT E14 CDX2 ChIP-seq replicate 1; Mus musculus; ChIP-Seq
library_construction_protocol: E14 small intestines were digested in 0.25% Trypsin EDTA at 37°C for 30 minutes followed by neutralization with FBS and Epcam + cells were sorted by flow cytometry. Cells were immediately cross-linked and snap frozen for subsequent ChIP-seq. Whole cell lysates were sonicated and incubated with 30 uL CDX2  antibody (#12306, Cell Signaling) overnight for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.