title: GSM3763562: WT_0.1_rep2; Komagataella phaffii; RNA-Seq
library_construction_protocol: The ethanol/phenol fixed cells were resuspended in 1 mL TRI reagent (Sigma Aldrich), lysed using glass beads as described in the previous section and RNA was extracted according to the TRI reagent protocol. DNase treatment was carried out using the Ambion DNA-free kit (Invitrogen). RNA concentration and integrity was analysed using the Nanodrop spectrophotometer and Bioanalyzer (Agilent). mRNA library preparation, polyA enrichment