title: GSM2354124: ZmCENH3_rep2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the KAPA Hyper prep kit for Illumina (Part# KK8504). Briefly, end-repaired and adaptor ligated DNA was amplified for 5 cycles. The resulting library was size-selected using BluePippin for 200-400 bp, quantified by qPCR to determine minimum number of additinoal amplification cycles needed.