title: GSM4365650: col3WL; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Whole seedlings material of 30 seedlings per sample were collected in 1.5ml eppendorf tubes.Plant materials were ground using silamat and RNA was isolated with Plant RNeasy kit (Qiagen) and treated with DNaseI according to the manufacturer's instructions.DNase was heat inactivated in the presence of 2 mM EDTA.The libraries were prepared with 400 ng of total RNA as starting material and processed with the TruSeqUD Stranded mRNA (Illumina), and sequencing on an Illumina HiSeq 4000 System using 100 bp single-end reads protocol. RNA libraries were prepared for sequencing using standard Illumina protocols