title: GSM1431894: Foetal Primordial Germ Cells (PGCs); Mus musculus; ncRNA-Seq
library_construction_protocol: Total RNA from Oocytes, Zygotes, Spermatozoa, Spermatogonia and Primordial Germ Cells (PGCs) was isolated using TRIzol® Reagent (Invitrogen) according to the manufacturer's instructions.To purify fetal PGCs and postnatal SPGs, a cell suspension was prepared from 13.5 dpc male gonads and 8.5 dpn testes, respectively. RNA libraries were prepared for sequencing using standard Illumina protocols. Illumina sequencing reported sequences 18-32 nucleotides long.