title: Leaf_transcriptome
design: Six different genotypes (2 resistant, 2 susceptible and 2  parents) were selected for RNA-seq analysis based on phenotypic results. The  growing condition of the plants, inoculum and inoculation were performed as  described above. The experiment was arranged in three replicates and samples  were collected at 10 and 12 days post inoculation (dpi). At each time point,  two leaflets were collected from each individual plant pooled and the  collected samples (totally 36) were immediately frozen in liquid nitrogen and  stored at -800C until further processing. Total RNA was isolated from 100 mg  of frozen tissue with the RNeasy Plant Mini kit (Qiagen) including DNase  treatment (RNase-free DNase set, Qiagen) based on the manufacturers  instructions. RNA quality and concentration were estimated in ExperionTM  Automated Electrophoresis System (Bio-Rad Laboratories, Hercules, USA). cDNA  library construction and sequencing of the samples were done at the  SciLifeLab (Stockholm, Sweden).