title: Nanopore sequencing of Thamnaconus septentrionalis
design: To generate Oxford Nanopore long reads, approximately 15 g of genomic DNA was size-selected (3080 kb) with a BluePippin (Sage Science, Beverly, MA, USA), and processed according to the Ligation Sequencing Kit 1D (SQK-LSK109) protocol. Briefly, DNA fragments were repaired using the NEBNext FFPE Repair Mix (New England Biolabs). After end-reparation and 3-adenylation with the NEBNext End repair/dA-tailing Module reagents(New England Biolabs), the Oxford Nanopore sequencing adapters were ligated using NEBNext Quick Ligation Module (E6056) (New England Biolabs). The final library was sequenced on 3 different R9.4 flow cells using the PromethION DNA sequencer (Oxford Nanopore, Oxford, UK) for 48 hours.