title: GSM1149547: SKM_rep1_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total cellular RNA was extracted with TRIzol reagent (Sigma). Genomic DNA were removed with DNase (Ambion). Amplified single cell cDNAs were further amplied for another ten cycles of PCR. Then it was sonicated into 200~500bp fragments, then the standard TruSeq DNA library preparation kit was used following the suggested protocol in the manual.