title: GSM3872500: Sham3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol followed the manufactor's protocol. RNA was then examined by Bioanalyzer RNA nano chip. RNA with RIN>8 was used for library contrusction. RNA libraries were prepared using NEB Ultra II RNA library prep kit and amplified with single end primers. Libraries were sequenced with illumina H4K (50SE).