title: GSM3181617: Liver_control_110d_male_6; Rattus norvegicus; RNA-Seq
library_construction_protocol: F1 liver tissue samples (10-20 mg) were homogenized with the BioSpec BeadBeater and RNA was extracted using the QIagen miRNeasy mini kit (Qiagen), according to the manufacturer's instructions for n= 6 controls and n= 5 MO for each sex. RNA isolated quantity and quality were determined by using a Nanodrop spectrophotometer (Nanodrop Technologies, DE, USA) and stored at -80°C until use. cDNA libraries were prepared from 1 μg total RNA using an Illumina TruSeq RNA LS Sample Preparation kit v2 according to the manufacturer's instructions (Illumina, San Diego, CA, USA). Final individual cDNA libraries were assessed for quality and fragment size using the Agilent DNA 1000. Sequencing libraries were quantified with KAPA Library Quantification kit for Illumina platforms.