title: GSM1551302: changli-1; Vitis vinifera; RNA-Seq
library_construction_protocol: Approximately 50 berries were randomly selected from a 300-berry biological replicate for RNA extraction. Total RNAs for RNA-seq analysis and real-time polymerase chain reaction (PCR) experiments were extracted from frozen deseeded berries using a General Plant Total RNA Extraction Kit (BioTeke, China), and further incubated with DNase I (Promega, USA) according to the manufacturer’s instructions. RNA integrity and quality were assessed by analysis with the Aglient 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard Illumina protocols