title: GSM4575750: MOE_051916_DS11; Mus musculus; RNA-Seq
library_construction_protocol: Briefly, dissected main olfactory epithelium were cleaned up in 750 µl of EBSS (Worthington) and epithelium tissues were isolated in 750 µL of Papain (20 U/mL in EBSS) and 50 µL of DNase I (2000 U/mL). Tissue pieces were transferred to a 5 mL round-bottom tube (BD) and 1.75 mL of Papain and 450 µL of DNase I were added. After 1–1.5 hour incubation with rocking at 37°C, the suspension was triturated with a 5 mL pipette 15 times and passed through 40 µm cell strainer (BD) and strainer was washed with 1 mL of DMEM + 10 % FBS (Invitrogen). The cell suspension was centrifuged at 300g for 5 min. Cells were resuspended with 4 mL of DMEM + 10 % FBS and centrifuged at 300g for 5 min. Cells were suspended with PBS + 0.01 % BSA and concentration was measured by hemocytometer. Drop-seq experiments (cell capture, reverse transcription, PCR, tagmentation), were performed as described in Macosko et al. 2015. Microfluidics devices were obtained from FlowJEM and barcode beads were obtained from Chemgenes. 8 replicates of Drop-seq samples were sequenced across 5 runs on an Illumina NextSeq 500 platform.