title: GSM3767542: CLIP-seq ZAK-alpha-RBR; Homo sapiens; OTHER
library_construction_protocol: After pull-down, RNA was extracted using PCI and chloroform and precipitated with 96% ethanol. Adaptors were added to the fragmented RNA using a system relying on a modified Arabidopsis tRNA ligase joining 2',3' cyclic phosphate and 5' phosphate ends. The library fragments were then sequenced on the Ion Proton platform.