title: GSM3179799: DAOY 8-hour, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Electrotaxis channels were scraped and the cells and Matrigel collected into 600 μL of cell recovery solution (BD, now Corning 354253) and kept on ice for 30 min. Technical replices (n=3) were pooled and centrifuged for 5 min at 800xg, supernatant removed and stored at -80ºC. RNA was extracted using a RNeasy Micro Plus Kit (Qiagen 74034). Purity and yield was assessed on a Nanodrop 2000 (Thermo-Fisher). RNA integrity was assessed with an Agilent Bioanalyzer RNA 6000 Nanokit on an Agilent Bioanalyzer 2100. Library preparation was done with a TruSeq Stranded mRNA Sample Preparation Guide LT with polyA tail pull-down. Library was validated with an Agilent DNA 1000 kit after fragmentation and addition of adapters. Samples were cleaned with AMPure XP beads and normalized with fluorimetric quantification to 10nM using a Qubit 2.0 Fluorometer using Qubit Broad Range (ThermoFisher Q32850) and High Sensitivity (ThermoFisher Q32854) kits.