title: Alchemy probe capture
design: Illumina Sequencing Library Preparation  Genomic DNA was mechanically sheared using a UCD-200 Biorupter (Diagenode, Holliston, MA) at 30s on/off intervals for a total of 32 minutes resulting in an average fragment size of 250bp. Fragments were purified using AMPure XP beads (Beckman Coulter Inc., CA, USA) and size distribution confirmed using an Agilent D1000 Tape Station (Agilent Technologies Inc., CA, USA). Barcoded sequencing libraries were prepared using the TruSeq Nano gDNA HT sample preparation kit (Illumina Inc., San Diego, CA) according to manufacturer’s protocol. (TruSeq Nano DNA Library Prep Protocol Guide, 2015)