title: The ecological and genetic basis of convergent thick-lipped phenotypes in cichlid fishes
design: For library construction and sequencing, standard protocols were applied. Poly-A mRNA was selected using poly-T oligo-attached magnetic beads. The recovered mRNA was fragmented into smaller pieces using divalent cations under elevated temperature. cDNA was produced using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNaseH. cDNA went through an end repair process, the addition of a single ‘A’ base, and ligation of the adapters. It was then purified and enriched with PCR to create the final cDNA library