title: GSM3330473: BL20_0; Bos taurus; miRNA-Seq
library_construction_protocol: 100ml of cell culture was centrifuged at 300 x g for 10 minutes, 2000 x g for 10 minutes and 10,000 x g for 30 minutes, with cells and cell debris discarded at each stage. EV were then pelleted by ultracentrifugation using a Surespin 630 rotor (Sorvall) at 100,000 x g for 70 minutes, washed in sterile PBS and finally centrifuged at 100,000 x g for 70 minutes before resuspending in 100 ml of PBS. RNA libraries were prepared for sequencing using standard Illumina protocols by LC Sciences (Texas)