title: GSM4141934: MED10_dTAG_7_C; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using commercially available kits (RNeasy mini, Qiagen). For cell-count normalization, equal amounts (10µL of 1:100 dilution = 0.303ng) of synthetic mRNA spike-ins (SIRV-Set-3; Lexogen, Vienna, Austria) were added to QIAshredder-homogenized lysates after the first extraction step with buffer RLT. Total RNA was quantified using NanoDrop and diluted to 70ng/uL for library preparation. Quant-seq libraries were generated from 350ng RNA using a commercially available kit (Lexogen, Vienna, Austria). Samples were PCR-amplified for 13 cycles, yielding 0.67-3.16ng/µL DNA per sample. Samples were pooled to equimolar amounts for 50bp sequencing in single-read mode.