title: RNA-seq of mus musculus hearts: doxycline and oligomycin
design: First strand cDNA was synthesized using random hexamer primers. Second strand cDNA was synthesized incorporating dUTP for strand marking, followed by end repair to produce blunt-ended ds cDNA. Adenylation of the 3 ends was performed and platform-specific adaptors were ligated to the ds cDNA. Fragments were purified by gel electrophoresis, and those with adaptors on both ends were enriched by PCR targeting the adaptors, producing cDNA amplicons of about 200-500 base pairs.