title: RNA Virome from a pool of five cyanobacterial mat samples from Lake Limnopolar-watershed (Byers Peninsula, Livingston Island, Antarctica) in Jan 2008
design: RNA viruses were purified by centrifugation, filtration (0.45 um), sucrose cushion (25%) ultracentrifugation and nuclease treatment. Protected viral RNA genomes were extracted by Trizol followed by a treatment with DNase I RNAse free and then ramdomly amplified by Sequence Independent Single Primer Amplification.