title: DNA-Seq of bacterial community from Cave Ice
design: Raw sequences from DNA and cDNA dataset in fastq format where demultiplex and quality filtering using QIIME 1.9.1: Quantitative Insights Into Microbial Ecology, (Caporaso et al. 2010). Sequences were then clustered into OTUs using the Open-reference approach using silva_99 version 128, reverse strand match were set to true to allow the match with the reference database.