title: GSM4141790: GBM29; Homo sapiens; RNA-Seq
library_construction_protocol: Sorted tumor cells from human GBM specimens were washed once and resuspended in PBS containing 0.05% BSA. 21ul of a cellular suspension at 500 cells/ul with 95% viability, were loaded onto the 10X Genomics Chromium platform to generate barcoded single-cell Gel Bead In-Emulsions (GEMs). Single-cell RNA sequencing (scRNA-seq) libraries were prepared according to 10X Genomics specifications (Single Cell 3′ V2 Reagent Kits User Guide PN120233, 10x Genomics, Pleasanton, CA, USA).