title: GSM1151059: HCT116_6hr_TSA_GA3816_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total mRNA was isolated from all cells using the RNeasy kit (Qiagen) and converted to cDNA using the Superscript ds-cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions. For RNA-Seq, double-stranded DNAs were fragmented to 100–200 bp using sonication, followed by end-repair, Illumina adaptor ligation, and sequencing using the Illumina HiSeq 2000 platform according to the manufacturer’s protocol for paired-end reads.