title: GSM4141683: Nascent_RNA_Hexanediol_replicate_1; Homo sapiens; RNA-Seq
library_construction_protocol: 108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. DNA libraries were prepared using the NEB Next Ultra Directional RNA library preparation kit for Illumina (E7765).