title: GSM1428963: CENH3-ChIPseq-telo2-2; Zea mays; ChIP-Seq
library_construction_protocol: Extracted nuclei were digested with micrococcal nuclease (Sigma-Aldrich) for ChIP experiments using the maize CENH3 antibody.  The maize CENH3 antibody is a rabbit polyclonal antiserum and was raised against the peptides: C-RPGTVALREIRKYQKS; STSATPERAAGTGGR-C. Keyhole limpet hemocyanin (KLH)-conjugated peptide was used to immunize rabbits. Anti-CENH3 polyclonal antibodies were affinity-purified using the peptides conjugated to Sulfo-link coupling Gel (Pierce) performed by GL Biochem (Shanghai) Ltd. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).180-400 bp fragments (contained the joint) were selected by gel extraction.