title: mESC_smart_sc4
design: cDNA from single cells was produced following the manufacturer's                 protocol using the Smart-Seq2 v4 Ultra Low Input RNA kit (Clontech) for single                 cells. Then, 2 ng of cDNA was sheared into 150- to 350-bp fragments by sonication,                 and a NEBNext DNA library preparation kit (E7645) was used to construct the                 sequencing library.