title: GSM4013835: Heart (ventricle), UW4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from the lower half of the mouse heart ventricles was isolated using Ribozol. Four biological replicates were used per diet, each replicate represents myocardium from a single animal. Quality control of RNA samples were performed by tape station, samples with RIN > 7.0 were processed for library preparation and qPCR. Single-end (75 base pairs) sequencing was performed using an Illumina NextSeq 1x75 high output flow cell. Quality control, poly-adenylated mRNA isolation, library preparation, qPCR and sequencing were performed by the Harvard FAS center for systems biology. Libraries were generated from RNA using Kapa mRNA HyperPrep kit, to generate strand-specific, poly-A enriched libraries.