title: Whole genome resequencing of Limenitis arthemis: VT36: Sample VT36
design: "Mimetic and non-mimetic individuals were captured in Massachusetts and New Hampshire respectively, and allowed to oviposit in the laboratory. Mimetic and non-mimetic individuals were then crossed to siblings to ensure that they were true-breeding for each respective phenotype. Individuals from each cross were sampled at the 5th instar (n=3 mimetic; n=3 non-mimetic), prepupa (identified by stereotypic ""j-curve"" hanging from leaves; n=3 mimetic; n=3 non-mimetic), early pupation (<48h post-pupation; n=3 mimetic; n=3 non-mimetic), and late pupation (>48h post-pupation; n=3 mimetic; n=3 non-mimetic). Wing discs were dissected from each individual in cold PBS, wing discs were then stored in RNA-later (Ambion, Inc.) following manufacturer's instructions. RNA was extracted using an RNAeasy-kit (Qiagen, Inc), and individual RNA-seq libraries were prepared using the TrueSeq RNA sample prep kit (Illumina, Inc.) at the Michigan State University Genomics core facility. Each library pooled, and pools were sequenced across two lanes of an Illumina HiSeq 2500, using 2x150bp reads."