title: GSM3868471: shNS control w/ dox 7 days; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted using Trizol Reagent. RNA integrity was verified on a 2100 Bioanalyzer (Agilent Technologies). Libraries were prepared using ScriptSeq v2 RNA-Seq Library Preparation kits (Epicentre) and multiplexed with ScriptSeq Index PCR primers (Epicentre).  ERCC ExFold RNA Spike-In mix (Life Technologies) was added and rRNAs removed using Ribo-Zero (Epicentre).