title: GSM2166101: Sample 1: PFC_control_saline; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized; brains were removed, coronally sliced and NAC, PFC, HIP and AMY tissues were rapidly dissected and frozen on dry ice. Tissue from 2 mice were pooled for each sample for n=3-5 biological replicates for each brain region and phenotype. RNA was isolated with TriZol reagent (Invitrogen) and purified with RNAeasy micro kits from Qiagen. Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 protocol  with 1ug RNA (Illumina, San Diego, CA). Briefly, the cDNA was synthesized from poly-A-selected and then fragmented total RNA using random hexamers, followed by end-repair and ligation with   sequencing adaptors. The libraries were size selected and purified using AMPure XP beads (Beckman Coulter, Brea, CA). Barcode bases (6 bp) were introduced at one end of the adaptors during PCR amplification steps. Library size and concentration were measured by Bioanalyzer or Tape Station (Life Technologies, Grand Island, NY) before sequencing. Libraries were sequenced on the Illumina HiSeq 2500 System utilizing V3 chemistry with 50 base pair paired-end reads at the Mount Sinai Genomics Core Facility.