title: GSM1547653: P10.5 Morc1 KO Replicate 3 RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from whole testes or cells using the RNeasy Micro Kit (Qiagen 74004). The material was quantified using a Nanodrop ND-1000 (Nanodrop) for RNA from whole testis or the Qubit RNA High Sensitivity Assay (Life Technologies) for RNA from sorted germ cells. RNA quality for material from whole testis was assessed by gel electrophoresis and visualization of the 28S and 18S rRNA bands. RNA from whole testes was processed for sequencing using a TruSeq RNA Sample Preparation Kit v2 (Illumina) with 250ng - 2μg total RNA as starting material. Mutant and controls were always matched for starting RNA content. RNA from sorted germ cell was processed using the Ovation Human FFPE RNA-Seq Multiple kit (Nugen) using custom primers for depletion of murine rRNA provided by the manufacturer, using 10ng of total RNA. Each library was prepared using RNA from one individual mouse.