title: GSM4011113: A549, infected with H3N2-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from infected cells using SuPerfecTRITM Total RNA Isolation Reagent (Pufei) according to the manufacturer. RNA-seq was performed using an Illumina Hiseq 3000 (Illumina, United States) to get the raw data (raw reads). With the reads containing poly-N, adapter and low-quality being removed, clean data (clean reads) were obtained and the paired-end reads were aligned to human reference genome (version: Homo_sapiens.GRCh38)  . LncRNAs were identified using four predicted tools including predictor of lncRNAs and mRNAs based on k-mer scheme (PLEK) (Li et al., 2014), Coding-Non-Coding-Index (CNCI) (Sun et al., 2013) and Coding Potential Calculator (CPC) (Kong et al., 2007)，Coding Potential Assessment Tool (CPAT)(Wang L. et al., 2013). StringTie was used to calculate the FPKMs (expected number of Fragments Per Kilobase of transcript sequence per Million base pairs sequenced) of both lncRNAs and mRNAs in each sample. RNA-seq and data analysis was completed by ShangHai Genergy Biotech (Shanghai, China)