title: ITS2 sequencing of Quartz: 2.5 month timepoint
design: We used a two-step PCR to prepare amplicon libraries. For the first step, primer sets used for amplification of the 16S rRNA genes were 515F/808R (bacterial and archaeal 16S rRNA gene v4 region). For the second step, barcoded phasing primers were used to increase base diversity in sample library sequences.