title: PARE-seq of zea mays: immature tassel total polysomes
design: After total polysome isolation by ultracentrifugation, total RNAs were extracted from the total polysomes of maize (Zea mays, inbred line B73) immature tassels with TRI Reagent (Molecular Research Center, USA) and used for PARE (parallel analysis of RNA end) library construction according to the method describe by Zhai et al. [Zhai J, Arikit S, Simon SA, Kingham BF, Meyers BC. Rapid construction of parallel analysis of RNA end (PARE) libraries for Illumina sequencing. Methods. 2014;67:8490.].