title: Fungal Survey of the Lung
design: 16S and Fungal tag sequencing from healthy and immunocompromised human airway.
library_construction_protocol: Amplicon libraries were constructed by amplifying the ITS1 region of the fungal genome. Thermocycler protocols from ITS1 were described by Dollive et al 2012. All sequencing was preformed on a 454 GS Junior (Roche).