title: GSM1149490: Lu019; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Lysates were clarified from dissected Drosophila ovaries or HeLa cells and RNA-Sm protein complexes were isolated with antibody. RNA was purified from the immunoprecipitate, reverse transcribed and made into doublestranded DNA.  Libraries were prepared according to Illumina's instructions. Briefly, DNA was fragmented, ligated to adapters and PCR amplified with Illumina primers for 15 cycles. The resultant DNA was purified from an agarose gel and captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.