title: GSM1150320: RnaSeq_2.3Ptf1aGFP_NT; Mus musculus; RNA-Seq
library_construction_protocol: Mouse neural tubes were dissected from E12.5 2.3Ptf1a-GFP embryos into DMEM/F12 on ice and dissociated in 0.25% trypsin for 15 minutes at 37°C. Trypsin activity was quenched with 2% fetal bovine serum, and GFP positive cells were purified from the resulting single cell suspension by fluorescence activated cell sorting. Total RNA from the sorted cells was extracted and purified with Zymo’s Mini RNA Isolation Kit.For the whole E15.5 pancreas, total RNA was isolated using Trizol(Invitrogen). Neural tube and pancreata libraries were made according to Illumina’s mRNA-seq sample prep protocol. Pancreas libraries were made according to Illumina’s Tru-seq mRNA sample prep protocol