title: mESC_dual_small_sc1
design: A single cell was lysed with 200 ng of the carrier RNA mixture                 (containing 100 ng 5'-phosphorylated small carrier RNA mimic). The poly-A RNAs were                 selected using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), and                 the small RNAs remained in the supernatant after poly-A selection. The small RNA                 sequencing library was constructed following the manufacturer's protocol using a                 NEBNext kit (NEB #E7300). After PCR amplification (15 cycles), the cDNA fragment                 from the RNA carrier was removed by Not I digestion. After Not I digestion, the                 library DNA was purified for deep sequencing.