title: GSM3573284: IMR90_0Gy_24h_1_S7_R1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation was performed with the RNeasy Mini kit (Qiagen, RNeasy Mini Handbook 06/2012) following the manufacturer's protocol. RNA Seq library preparation was performed with Illumina TruSeq® Stranded mRNA Sample Preparation Kit (Cat.-No. RS-122-9004DOC, Part # 15031047 Rev. E, 10/13, Index Primers Set 1). Library fragment length was determined with Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) and concentration was measured with a Qubit 3.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). Libraries were sequenced with 2 x 75 bp paired end reads following the protocols for the NextSeq500 sequencing platform (NextSeq 500 High Output Kit 2x75bp; NextSeq System Guide; NextSeq Denature and Dilute Libraries Guide; Illumina, San Diego, CA, USA, Document # 15048776 v02, Document # 15046563 v02).