title: GSM1429728: EG-smallRNA-2; Echinococcus granulosus; miRNA-Seq
library_construction_protocol: For small RNA sequencing, an RNA preparation enriched in < 200nt RNAs was obtained from each sample using the mirVana miRNA Isolation Kit (Ambion, USA). The 18-30nt fraction of total RNA was excised and purified following 15% Tris-Borate-EDTA (TBE) denaturing polyacrylamide gel electrophoresis (PAGE). Subsequently, proprietary adapters were ligated to the 5’ and 3’ termini of the RNA using T4 RNA ligase respectively. The adaptor-ligated small RNAs were subjected to RT-PCR amplification, and after the cDNA was further amplified, the PCR products (90bp, small RNA+ adaptors) were purified using PAGE. Two biological replicate small RNA libraries were constructed for each species and were sequenced on Illumina HiSeq™ 2000 Sequencing System at the Beijing Genomics Institute (BGI), Shenzhen, P.R.China.