title: HEK293T cells transfected with vectors encoding NP, PA, PB1 and PB2 of strain WSN and pHW2000-Pan M, 24h, replicate 2
design: Total RNAs were extracted using TRIzol reagent (Life Technologies). Truseq Stranded mRNA sequencing libraries were prepared with 500 ng total RNA. The libraries were sequenced on HiSeq 4000 platform in 2x151-nt paired-end mode.