title: Bacterial community composition of permaforst thaw ponds in subarctic Quebec: Sample NAS_H_Small_Surface
design: "DNA was extracted using the PowerWater Sterivex DNA Isolation Kit (MO BIO Laboratories Inc., USA). V6-V8 regions of bacterial 16S rRNA converted to cDNA were PCR amplified using forward primer B969F (5'-ACGCGHNRAACCTTACC-3') and individually bar-coded reverse primers BA1406R (5'-ACGGGCRGTGWGTRCAA-3'). The resulting 22 Biosamples were sequenced using Roche/454 GS FLX technology."