title: RNA-seq of pine needles after UV-C
design: Frozen stem samples were ground to fine powder using a milling machine, total RNA extracted and depleted for ribosomal RNA. Thereafter ligation of SOLiD adaptor mix , reverse transcription, size fractionation to 150-200 bp cDNA, amplification and purification according to the protocols described in SOLiD Total RNA-Seq Kit (Invitrogen, USA).