title: GSM3574585: 171218_A3_A6_S3 [VF-005]; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were collected in 350 μL of TRK Lysis Buffer supplied with the E.Z.N.A. Total RNA Kit I (Omega Bio-tek, Norcross, GA) after 48 hours and stored in - 80°C. RNA was then isolated according to the manufacturer's instructions. Using the GoScript™ Reverse Transcription System (Promega, Madison, WI) according to the manufacturer's instructions, RNA was converted into cDNA. Libraries  were prepared using Illumina® TruSeq® DNA Sample Preparation Kits high-throughput protocol.