title: GSM3181709: WT Villus H3K4me1 ChIP-seq replicate1; Mus musculus; ChIP-Seq
library_construction_protocol: Villi from proximal 1/3rd of mouse small intestine were isolated by incubating in 5mM EDTA in PBS for 45 minutes at 4°C. Cells were cross-linked and immediately snap-frozen in liquid nitrogen for subsequent ChIP analysis. Whole cell extracts from villi fixed with 1% formaldehyde were sonicated and incubated overnight with 2 ug H3K4me1 antibody (C15410194, Diagenode) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.