title: GSM3030383: Patient BS379 timepoint N4; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs were isolated from freshly drawn whole blood by histopaque density centrifugation and frozen to liquid nitrogen until further use. Afterwards cells were thawed and stained with fluorescently labeled antibodies for markers of CD8+ T cells (CD45 CD3 CD8) and CD8+ T cells sorted directly into RNA lysis buffer. RNA was isolated using the SMARTer Universal Low Input RNA Kit (TaKaRa/Clontech). Illumina TruSeq Nano