title: RNA-seq of Drosophila yakuba: adult male
design: Total RNA was isolated from either 30 males or 15 females wit Trizol, treated with RNase-free DNase I in solution and cleaned with RNeasy Mini Kit.  Total RNA was purified using using poly-T oligo-attached magnetic beads, fragmented at elevated temperature, and retrotranscribed to cDNA.  Size selection: 250bp; Agarose gel;  Multiplexing for sequencing using custom-designed adapters with Tags.  Tags have been eliminated in the fastq files.