title: GSM2692650: Sync1; Homo sapiens; RNA-Seq
library_construction_protocol: After a release (35-45min), mitotic cells were collected by mitotic shake-off and treated without adding RNases to the lysis buffer Library generation was done with the kit NEBNext Ultra RNA Library Prep Kit (New England Biolabs), following manufacturer´s instructions and 14-15 cycles of library amplification. Previous Poly A purification was only performance on Input samples in only one step of purification. Size selection of the libraries and purification were done by magnetic beads (Agencourt AMPure XP, Beckman Coulter). Finally, library quantification was performance with Qubit DNA HS (Life Technologies) and fragment size distribution with Bioanalyzer DNA HS (Agilent)