title: Illumina of c-DNA from bacteria isolated from worms
design: For each sample, a 464-nucleotide sequence of the V3-V4 region, of the 16S rRNA gene  was amplified starting from the amplified c- DNA. Unique barcodes were attached before the forward primers to facilitate the pooling and subsequent differentiation of samples. The amplicons were cleaned using the Agencourt AMPure kit; subsequently, DNA concentrations of the amplicons were determined using the Quant-iT PicoGreen dsDNA kit . The quality of the generated amplicon libraries was evaluated by a Bioanalyzer 2100  using the High Sensitivity DNA Kit (Agilent). Following the quantitation, cleaned amplicons were mixed and combined in equimolar ratios.