title: GSM1548250: 30 ' following memory retrieval rep3; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction was performed using Qiagen RNAeasy Microarray Tissue kit. All RNA extractions were performed the same day within a week of tissue collection. 2µg of RNA was used for library preparation using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) with Poly A selection according to the manufacturer’s instructions. Completed libraries were size-selected (200-400 bp) on an agarose gel to remove any high basepair fragments, quantified by qPCR (KAPA Biosystems, Boston, MA), and submitted to the Penn Genome Frontiers Institute (PGFI) sequencing core at UPENN for sequencing. 3 libraries were multiplexed per lane (one biological replicate of each 3 conditions) and sequenced on an Illumina HiSeq 2000 using Type 3 chemistry, resulting in an average of 64 million 100 bp pair-end reads per sample.