title: GSM2910641: OT_C_F12; Mus musculus; RNA-Seq
library_construction_protocol: Mouse skin squamous cell carcinoma cells including tumor initiating cells before and after adoptive cell transfer (ACT)-based immunotherapy were FACS-purified, collected into 96 well plates containing lysis buffer and stored in -80oC Single cells were lysed by incubating at 72oC for 3 min. Revers transcritpion reaction and whole transcriptome amplification were perforemd according to Smart-seq2 protocol with a few modifications (Yang et al., Cell, 2017). The amplified cDNA libraries were purified by Ampure XP beads (Beckman Coulter), and tagmentation reaction was perforemd by Illumina Nextera XT DNA Library Prep Kit. The libraries were pooled and sequecned on Illumina Nextseq 500 with mid-output 75cycles paired-end mode.