title: GSM3863306: MCF7-ATAC-shCTRL; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 sorted cells were washed with cold PBS and suspended in lysis buffer for 10-15 min on ice.  DNA was purified using MinElute PCR Purification Kit (Qiagen). Transposed DNA was amplified using NEB Next High-Fidelity 2X PCR Master Mix and libraries were sequenced on HiSeq 4000 DNA libraries were prepared for sequencing using standard Illumina protocols