title: GSM3331033: ATACseq_subject1_abd_rep2; Homo sapiens; ATAC-seq
library_construction_protocol: After trypsinisation, abdominal and GF preadipocytes nuclei were isolated Nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina). After tagmentation DNA was purified with MinElute PCR Purification Kit (Qiagen). Tagmented DNA was amplified with Kapa Hifi Hot Start Kit (Kapa Biosystems) using 16 PCR cycles. Amplified libraries were purified with Agencourt AMPure XP (Beckman Coulter)