title: Tollipmicrobiome
design: The PCR products were purified after 1% agarose gel electrophoresis using Gel Extraction Kit (Qiagen) according to manufacturers instructions. Purity and concentration of the amplicon were determined using Qubit 3.0 Fluorometer (Thermo Fisher Scientific) prior to library preparation for sequencing on the IonTorrent PGM system (Life Technologies). Libraries were diluted according to manufacturers instructions and processed using the Ion PGMTM Hi-QTM View OT2 400 kit (Thermo Fischer Scientific) (for up to 400 bp libraries) and the Ion PGMTM Hi-QTM View Sequencing kit (Thermo Fischer Scientific) for sequencing on an Ion 316TM V2 chip (Thermo Fischer Scientific)3.