title: GSM3576255: Patient2-first; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using PAXgene Blood RNA Kit (Qiagen, Hilden, Germany), including DNase I treatment according to the manufacturer's instructions. The eluted RNA was again treated with DNase I and cleaned using the RNA Clean & Concentrator kit (Zymo, Irvine, CA) to remove trace amounts of genomic DNA. The RNA quality was measured by using Bioanalyzer RNA 6000 Nano Kit (Agilent, Santa Clara, CA). Globin-Zero Gold kit (Illumina, San Diego, CA) was used to enrich non-globin coding and non-coding RNAs from blood-derived total RNA. To ensure high reproducibility of the process, the automated SMARTer Apollo system (Takara, Mountain View, CA) was used to deplete globin and rRNA from 1 µg total RNA. The NEBNext Ultra Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA)  was used for library preparation.