title: 2b-RAD of Nymphon australe: muscle tissue
design: Genomic DNA preparation followed the 2b-RAD protocol from Wang et al. (2012). Digestion of genomic DNA utilized the restriction enzyme AlfI. Two site-specific ligation adaptors (NC/NG) were used to employ a   reduction scheme. The adaptors bind to the two base-pair sticky end products of the AlfI restriction. Samples were dual barcoded with unique combinations before being sent for sequencing at HudsonAlpha Institute for Biotechnology Genome Services Laboratory (Huntsville, Alabama).