title: Diversity assessments from microbes to mammals with eDNA metabarcoding in the Monterey Bay National Marine Sanctuar
design: This protocol is aimed at amplifying the 18S rRNA hypervariable region 9 (18S V9) in eukaryotes. The primers (1391F and EukBr) utilized in this protocol are based on the primers from Amaral-Zettler et al 2009 and the Earth Microbiome Project (EMP).