title: PGM Sequencing Data for NIST MG004 Clostridium sporogenes - Vial 0 run 02
design: Independent libraries were constructed from 8 replicate Clostridium sporogenes microbial genomic DNA reference material (MG004) vials the libraries were pooled and sequenced in a single run.  Libraries were prepared using the Applied Biosystems automated Library Builder system using the Ion Xpress Plus kit (lot #1676689 ), including the Ion Xpress plus fragment plus library kit, with a 318C.  The 8 vials were barcoded using the IonXpress Kit (vials numbers correlate with MiSeq data vial numbers).  The template was prepared using the Ion Chef (Kit Lot #1742719A) and the Ion PGM Hi-Q kit was used for sequencing. Base calls were made using the Torrent Suite version 4.4.2