title: GSM3764464: ChIP_EDL_Med1; Mus musculus; ChIP-Seq
library_construction_protocol: Skeletal muscle was dissected and finely minced. ChIP-seq samples were subjected to cross linking in 1% formaldehyde for 30 minutes and quenched with glycine. Tissue was dounced to release nuclei. Nuclei were sonicated to shear the chromatin and subjected to immunoprecipitation. Precipitates were washed and DNA was purified by column (Qiagen Min-Elute). ATAC-seq samples were cryo-pulverized and 30mg of powdered tissue was subjected to transposase reaction as per Buenrostro et al 2013. ChIP-seq libraries were prepared with the KAPA Hyper Prep Kit (Kapa Biosciences). ATAC-seq libraries were prepared as per Beunrostro et al 2013.