title: RNAseq of Glycine max:  DAI3 infected
design: Approximately 500mg fresh weight of infected and uninfected root tissue was harvested from each of three replicate plants at 3, 6, 9, and 12 days after inoculation (DAI), a total of 24 samples (2 tissue types x 4 dates x 3 biological replicates). Tissue was stored in RNAlater® Stabilization Reagent (Ambion, Austin, TX) and transported to the University of Arizona Genetics Core (Tucson, AZ) for 100-bp paired-end library preparation, barcode tagging, and sequencing on two lanes of the Illumina HiSeq 2000 platform (Illumina, San Diego, CA).  Read quality was assessed with FastQC v0.10.1.  Read were processed by Trimmomatic v0.32 and ConDeTri v2.2.