title: Hi-C of Thamnaconus septentrionalis
design: The cells of muscle tissue were fixed with formaldehyde and restriction endonuclease Hind III was used to digest DNA. The 5 overhang of the fragments were repaired and labeled using biotinylated nucleotides, followed by ligation in a small volume. After reversal of crosslinks, ligated DNA was purified and sheared to a length of 300-700 bp. The DNA fragments with interaction relationship were captured with streptavidin beads and prepared for Illumina sequencing. The final Hi-C libraries were sequenced on an Illumina HiSeq X Ten platform to obtain 2  150 bp paired-end reads.