title: RNA-seq of Glycine max: leaf abscission zone 48h ethylene replicate 1
design: Soybean (Glycine max, cv. Williams82) plants were grown in the greenhouse and harvested when the primary leaves were fully expanded (19 to 24 days).  Stem-petiole explants were prepared by removing all but approximately a 5-mm triangular portion of the leaf blade, and cutting the stem approximately 4 cm below the node.  Explants were placed in Erlenmeyer flasks with water and put into a darkened chamber wherein 25 microL/L ethylene in air saturated with water was passed through at a rate of 2 L/min at 25 C.  At 0, 12, 24, 48, and 72 h, approximately 2-mm abscission zone tissue (AZ) located just below the leaf blade was collected from 20 explants (2 AZs per explant or 40 AZs total), and the same number of petioles with the AZ removed (NAZ-pet) collected.  