title: RNA-seq of submerged iris leaves in wastewater
design: A culture experiment was conducted in a glasshouse at Hangzhou,China in 2018.Two treatments were conducted in this culture:a plant (Iris tectorum) culture and a plant-algae (Iris tectorum-Dictyosphaerium sp.) co-culture by using swine wastewater as the culture media.A complete randomized design with three replications was used.The culture containers were 4L transparent beaker and each container contained 3L of working solution.The submerged iris leaves were collected at day 6, and immidiately frozen in liquid nitrogen,ground into powder and stored at -80 degres Ces.Total RNA was then extracted following the manufacter's instructions.An Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano kit) was used to determine QC:RNA concentrationss, RIN values,28S/18S, and fragment length distributions.A NanoDropTM spectrophotometer was used to assess the purity of the RNA.Aliquots from mRNA samples were used to construct cDNA libries.