title: GSM1546452: mP2; Mus musculus; RNA-Seq
library_construction_protocol: Media was removed from Matrigel domes, and 4-6 wells of organoids were harvested in 1 mL total of Trizol. RNA was extracted using the Trizol Plus RNA Purification Kit (LifeTechnologies) per manufacturer’s instructions, and RNA samples were treated on column with PureLink DNase (Life Technologies). Sequencing libraries were generated from 1 ug of total RNA using a TruSeq Stranded Total RNA Kit with Ribo-zero human/mouse/rat (Illumina # RS-122-2201) per manufacturer’s instructions. For the final amplification,11 cycles of PCR were used. Libraries were quality checked and quantified using a Bioanalyzer 2100 (Agilent) with a DNA 1000 Kit.