title: Illumina HiSeq 2500 sequencing; RNA-Seq analysis of Arabidopsis heterozygous mutant of gene (AT1G31870), which encodes a pre-mRNA-splicing factor of RES complex protein against the wild type (Col)
design: RNA-Seq analysis of Arabidopsis heterozygous mutant of gene (AT1G31870), which encodes a pre-mRNA-splicing factor of RES complex protein against the wild type (Col)
library_construction_protocol: The siliques at 0, 1 and 2 days after pollination of wild type and atbud13-/+-1 plants grown on LD conditions were pooled  together and used for Solexa Sequencing Arabidopsis plants of wild type and mutant grow under normal long day conditions (16 h light/ 8 h dark).   Total RNA was isolated using mirVana™ miRNA ISOlation Kit (AM1561, Thermo Scientific, Wilmington, Delaware, USA) The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (RS-122-2101, Illumina, San Diego, CA, USA) according to the manufacturer's protocol.