title: mESC_small_sc10
design: A single cell was lysed with 100 small carrier RNA. The small RNA                 sequencing library was constructed following the manufacturer's protocol using a                 NEBNext kit (NEB #E7300). After PCR amplification (15 cycles), the cDNA fragment                 from the RNA carrier was removed by Not I digestion. After Not I digestion, the                 library DNA was purified for deep sequencing.