title: RNA-seq of Ziziphora bungena
design: For SMRT-based RNA sequencing, the RNAs from root, stem, leaf, inflorescence and infructescence with three repeats were mixed as one sample for Pac-Bio library construction. The full-length cDNA was synthesized by using a SMARTer PCR cDNA Synthesis Kit (Clontech, USA), and the cDNA library was constructed using the SMRTbellTM Template Prep Kit 1.0 following the manufacturers instructions, and then the library was sequencing using the SequelTM Sequencing kit 2.0 with a PacBio Sequel systemPacific Biosciences, USA.