title: mRNAseq of Varanus komodoensis: adult female blood
design: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturers protocol from blood samples of three females and two males of Varanus komodoensis, a female of V. macraei and a female of V. beccari. Libraries were constructed by GeneCore (EMBL, Heidelberg, Germany). The barcoded stranded mRNA-sequencing libraries were prepared using the Illumina TruSeq mRNA v2 sample preparation kit (Illumina, San Diego, CA, USA) with poly-A mRNA enrichment, implemented on the liquid handling robot Beckman FXP2. The resulting libraries were pooled together and loaded on the Illumina NextSeq 500 sequencer and 84 base pairs (bp) were sequenced unidirectionally.