title: GSM4292641: CXCR2 neutrophils; Mus musculus; RNA-Seq
library_construction_protocol: Eyes were dissected, and the vitreous fluid was collected Retinas were rinsed with 200 ul of PBS and cells collected from rinse. Neutrophils were purified by MACS Ly6G bead purification. Cell purity and viability was confirmed by flow cytometry. The cDNA libraries were constructed using the 10x ChromiumTM Single cell 3' Library Kit according to the manufacturer's original protocol. Libraries were sequenced on an Illumina Novaseq paired-end kits using the following read length: 26bp Read1 for cell barcode and UMI, 8bp I7 index for sample index and 98bp Read2 for transcript.