title: daphmag2010genome454 paired end library insert 8kb
design: Genomic DNA was obtained for Daphnia magna, isolate XInb3, with PicoGreen quantification results. Quality control by Agilent DNA7500 chip and by Agilent mRNA Pico chip were done. After library construction, the sstDNA was analyzed using OliGreen. Titration emPCR was performed to find the appropriate copy per bead for the bulks runs, with 1.0 to 2.0 cpb used for these.