title: WGS of Escherichia coli MG1655, plasmid pHC: ancestral population used to evolve six biological replicates at 37 degrees C and six biological replicates at 42 degrees C; S51
design: Total DNA (genomic DNA plus plasmid DNA) was isolated from archived cultures (1ml) using the Wizard Genomic DNA Purification kit (Promega).  Library was prepared using the Nextera XT DNA Library Prep Kit.