title: GSM4576266: single-cell_RNAseq_WT pancreas; Mus musculus; RNA-Seq
library_construction_protocol: After islet isolation,further digestion was performed with Accutase (A6964, Sigma) at 27 °C for 5 minutes and the cells were suspended in culture medium. Cells were then FACS sorted to select only live cells. 300 islets froma male WT mouse and 300 islets pooled from 2 male mutant mice 1 week or 5 weeks after Taf4 inactivation were used for scRNA-seq. Cell capture was  performed using  10X Genomics Chromium Analyzer. Single Cell 3ʹ Gene Expression libraries comprise standard Illumina paired-end constructs which begin with P5 and end with P7. 16  bp 10x Barcodes are encoded at the start of TruSeq Read 1, while 8 bp sample index sequences are incorporated as the i7 index read. TruSeq Read 1 and Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing. TruSeq Read 1 is used to sequence 16 bp 10x Barcodes and 12 bp UMI.