title: GSM3573385: Control_Replicate1-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Direct-zol RNA kit (Zymo research), treated with DNase I and mRNA was enriched by oligo(dT) magnetic beads (Invitrogen). Upon fragmentation, double-stranded cDNA was synthesized using random hexamer-primer and reverse transcriptase (Invitrogen) and purified with magnetic beads. End reparation and 3'-end single nucleotide adenine addition was then performed. Sequencing adaptors are ligated to the fragment and enriched by PCR amplification and size-selected (~200 bp). During the QC step, Agilent 2100 Bioanalyzer and ABI StepOnePLus Real-Time PCR are used to quantify the sample library. The resulting product was subjected to RNA-Seq on the Illumina HiSeqTM 4000, using 50 bp single reads.