title: GSM2692166: gdt1/gdt1 mutant; Candida albicans; RNA-Seq
library_construction_protocol: Total RNA samples were extracted using the TRIzol total RNA reagent (Tiangen Inc., Beijing, China) according to the manufacturer’s instructions. RNA integrity was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA-seq libraries were constructed using Illumina’s TruSeq RNA Sample Preparation Kit (Illumina Inc, San Diego, CA, USA). RNA sequencing, data analysis and sequence assembly were performed by Beijing BioMarker Technologies (Beijing, China). Preparation of the paired-end libraries and sequencing were performed following standard Illumina methods and protocols. The mRNA-seq library was sequenced on the Illumina Hiseq 2500 system.