title: RNAseq of Medicago truncatula npd1/2/4 Knockout: Roots Rep3
design: M. truncatula plants were inoculated with Ensifer meliloti strain 1021. Plants were grown in a growth chamber at 22C and with a 16 h photoperiod in conetainers arranged in a completely randomized design. Trays were watered with sterile deionized water twice a week and with Fahräeus medium lacking nitrogen once a week. Thirty days post inoculation, roots and nodules were harvested and flash-frozen in liquid nitrogen. Total RNA was extracted with a Qiagen RNeasy Plant Mini Kit and sequenced by the University of Minnesota Genomics Center.