title: GSM2913288: Control.3 [input small RNA]; Homo sapiens; miRNA-Seq
library_construction_protocol: Two lysis protocols were used: 1) skeletal muscle biopsies were cut into small pieces in RIP lysis buffer (Magna RIP Kit, Merck-Millipore, Burlington, MA) and homogenized by TissueLyser (Qiagen, Hilden, Germany); 2) Cultured cells were lysed with the following buffer: 150mM KCl, 25 mM TrisHCl pH 7.4, 5 mM EDTA, 0.5% NP40, 5 mM DTT, 1 mM PMSF, protease and RNase inhibitors. For both lysates, homogenates were quantified for protein content, snap frozen in liquid nitrogen and stored at -80°C until use. For both muscle tissue and cells, 50 ug of lysate were used for total RNA analysis (INPUT RNA). Extraction and library preparation were performed following manufacturer's instructions for the TruSeq Small RNA Library Preparation Kit.