title: GSM3184809: H4K3 [BS-seq]; Homo sapiens; Bisulfite-Seq
library_construction_protocol: We prepared a total of 48 whole-genome bisulfite sequencing libraries (BS-seq) from extracted DNA as previously described (Tung et al. 2012, Banovich et al. 2014). For each sample, we prepared at least two libraries, with independent PCR amplifications to minimize PCR duplication rates. The BS-seq libraries were sequenced on 111 lanes on 17 flow-cells on an Illumina HiSeq 2500 sequencer in the Gilad lab or at the University of Chicago Genomics Facility