title: Whole-genome sequencing of Campylobacter jejuni
design: Isolates were grown on Columbia horse blood agar plates (Fort Richard Laboratories, Auckland, New Zealand) in a microaerobic atmosphere (85% N2, 10% CO2, 5% O2) at 42C for 24 hours in a VA500 variable atmosphere incubator (Don Whitely Scientific, Yorkshire, United Kingdom). DNA was extracted using a QIAamp DNA Mini kit (Qiagen, Hilden, Germany). The resultant DNA was then sent to the Massey Genome Service, Massey University, Palmerston North (part of New Zealand Genomics Ltd) for quality control checking, library preparation and sequencing. The isolates were sequenced on an Illumina MiSeq (250bp paired end run) to ~140x  200x estimated coverage based on the size of the Campylobacter jejuni genome.