title: GSM1149835: NCR335 10'; Sinorhizobium meliloti 1021; RNA-Seq
library_construction_protocol: Total RNA was purified from samples using the RiboPure Bacteria Kit (Ambion). Residual DNA was removed by using RQ1 RNase-Free DNase (PROMEGA). RNAs from the three biological replicates were pooled. Before library preparation, ribosomal RNAs were removed using the Ribo-Zero™ rRNA Removal Kit for Gram-Negative Bacteria (Epicentre). Library preparation and RNA sequencing were performed by using the dedicated kits and the SOLiD4 sequencer, respectively, from Life Technologies.