title: ChIP-seq low LP followed by high LP in Neurospora crassa
design: Cultures were grown 36 hours in light and transferred to constant darkness for 24 hours. Cultures were illuminated with 1 min low LP and challenged after 30 min with a high LP. Cultures were crosslinked and harvested 0, 5, 30 and 35 min after the first low LP. Ground mycelia was sonicated using a Covaris S220X sonicator (180 peak power, 20.0 duty factor, 200 cycles/bursts, for 4 min at 4°C). Total extracts were subjected to ChIP with specific affinity purified antibodies for RNA polymerase II. ChIP DNA libraries were prepared with NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® with NEBNext® Multiplex oligos.