title: GSM1548682: B97 Submerge 72 hr Rep 3; Zea mays; RNA-Seq
library_construction_protocol: RNA was extracted using the TRIzol method and quantified using Nanodrop spectrophotometer. RNA cleanup was performed according to RNeasy MinElute Cleanup kit protocol (Qiagen, Valencia, CA, USA). RNA was eluted with 15 µl RNase-free water after on-column DNase1 treatment using the protocol described by the manufacturer. Prior to library construction, RNA integrity was analyzed using Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, Calif.). Illumina small RNA library protocol