title: Genome of Indian Leishmania donovani
design: Illumina HiSeq 2000 platform was used to generate paired-end data ranging from 300-350 bp insert size .Fragmentation of 5 micro gram genomic DNA using a nebulization technique was carried out which generated double stranded DNA fragments comprising of 3'or 5'overhangs.The overhangs resulting from fragmentation were converted into blunt ens using T4 DNA polymerase and Klenow enzyme which added 'A' base to the 3'end of the blunt phosphorylated DNA fragments preparing the DNA fragments to be ligated to the adapters,which have single "T" base overhang at their 3'end .Enrichment of adapter modified DNA fragments by PCR was performed.