title: WGS of Homo sapiens: whole blood: Sample 8285
design: Whole-genome DNA sequencing libraries were constructed with 500 ng of genomic DNA isolated from blood, utilizing the KAPA HTP Library Prep Kit (KAPA Biosystems, Boston, MA) on the SciClone NGS instrument (Perkin Elmer, Waltham, MA) targeting 350bp inserts. Post-fragmentation (Covaris, Woburn, MA), the genomic DNA was size selected with AMPure XP beads using a 0.6x/0.8x ratio. The libraries were PCR amplified with KAPA HiFi for 4-6 cycles (KAPA Biosystems, Boston, MA). The final libraries were purified with two 0.7x AMPureXP bead cleanups. The concentration of each library was accurately determined through qPCR (KAPA Biosystems, Boston, MA). Twenty four libraries were pooled and loaded across four lanes of a HiSeqX flow cell to ensure that the libraries within the pool were equally balanced. The final pool of balanced libraries was loaded over an additional 16 lanes of the Illumina HiSeqX (Illumina, San Diego, CA). This efficient pooling scheme targeted ~30X coverage for each sample.   