title: Transcriptome analysis of the the two duplicate samples of Begonia semperflorens
design: A total of 3  g of RNA per sample was used as the input material for RNA sample preparation. Sequencing libraries were generated using a NEBNext Ultra  RNA Library Prep Kit from Illumina  (New England Biolabs, Ipswich, Massachusetts, USA) following the manufacturer s recommendations. Index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T-oligo-conjugated magnetic beads.