title: cloned DNA of Hordeum vulgare subsp. vulgare cultivar Balga: pooled F fraction of             shoots
design: DNA was (1) digested with Hind III and Pst 1 restrictases or                 alternatively with Alu I restrictase. The solution was pressed through                 nitrocellulose filter pre-soaked with filtration buffer supported in Swinnex filter                 holde. The filter retained DNA fraction enriched in the tightly bound proteins. The                 DNA content in filtered fraction (F), low-ionic strength eluted fraction (R1) and                 alkali-eluted fraction (R2) was measured spectrophotometrically. Cloning for                 sequencing was performed with pooled F fraction DNA (FF), coleoptile R1 and R2                 fraction DNA extracted from the coleoptiles, leaves and roots (CR1, CR2, LR1, LR2,                 RR1, RR2 fractions respectively).