title: GSM3031461: GBM cells; Homo sapiens; RNA-Seq
library_construction_protocol: The tissue was divided into several pieces, one of which was used for fresh dissociation and RNA-Best stabilization. The fresh tissue was first dissociated into SC suspension by mechanical and enzymatic papain dissociation as previously described (Tome-Garcia 2017 PMID 28434940 and https://bio-protocol.org/e2659) and then stabilized in RNA-Best at 100,000 cells per ml. SC Mini Drop-seq experiments were performed similarly as described in Macosko et al. (2015 PMID 26000488). Ficoll PM-400 was added to the cell buffer to match fluid viscosity of the aqueous flows. Nextera library preparation was performed as in the original manuscript by Macosko et al. (2015 PMID 26000488).