title: GSM4142014: TT-seq_MJ-19-29_MED14-dTAG_2h_dTAG7_30min_NVP2_B; Homo sapiens; OTHER
library_construction_protocol: TT-seq libraries were generated as previously described (PMID: 27257258). Cells were harvested by 3000g for 2min and total RNA extracted using Qiazol. RNA spike-ins were added and RNA was sonicated to generate fragments of <1.5 kb. 4sU-labeled RNA was purified using streptavidin beads (Miltenyi Biotec) before size-selection and library preparation. NuGEN Ovation Universal RNA-Seq System, starting with 100 ng RNA inputs.