title: GSM4197406: 05-Hete_rep-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA is extracted using Rneasy plus micro kit (Qiagen) with manufacturer's instructions. Total RNA obtained from each sample was subjected to a sequencing library construction using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA) with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) according to the manufacturer's protocols. Strand specific RNA-seq  (first strand).