title: RNA-Seq of  Sphaeridiotrema pseudoglobulus: adult worm (first)
design: The pooled samples of adult worms were fixed and stored in the IntactRNA (Eurogene, Moscow, Russia), according to manufacturer instructions. Prior to total RNA isolation, the intactRNA-fixed samples were rinsed in 0.1M phosphate-buffered saline (PBS). The total RNA isolation was done using Quick-RNA Microprep kit (R1050, Zymo Research, Irvine, California, USA).  The libraries were synthesized using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, New England BioLabs, Ipswich, Massachusetts, USA). Sequencing error correction was performed by Karect (v1.0). All libraries were checked for human tissue contamination (Encode: GRCh38, primary assembly) using BBTools packages (v37.02). Additionally, the Gallus gallus (Encode: Gallus gallus v5.0) reference genome was used for verification of adult worms libraries host tissue contamination.