title: Transcriptome of Vibrio vulnificus CMCP6
design: Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270.