title: RNA-Seq of Caulanthus amplexicaulis var. barbarae: whole plant transcriptome
design: RNAs were extracted using the RNAqueous® total RNA isolation kit (ThermoFisher). Genomic DNA was removed using the TURBO DNA-free kit (ThermoFisher). RNA integrity was assessed using the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA); all samples had a RIN (RNA Integrity Number) score of 7.0 or greater. The SMARTer® PCR cDNA Synthesis kit (Clontech, Mountain View, CA) was used to reverse transcribe total RNA into cDNA using a polyA-specific primer. A duplex-specific nuclease (Evrogen, Moscow, Russia) was used to normalize cDNA libraries (Zhulidov, et al. 2004) which were sent to the Genomic Sequencing and Analysis Facility (GSAF) at the University of Texas, Austin for paired-end (2x100 bp) sequencing using the Illumina Hi-Seq 2000 instrument.