title: GSM4087119: Fibroblast CRIST-seq Cas9 random gRNA (gCT) control; Mus musculus; OTHER
library_construction_protocol: Oct4 promoter-interacting lncRNA libraries were prepared using Illumines Kit (Part# 0801-0303). Fibroblasts were treated with formaldehyde to fix the lncRNA-chromatin structure. LncRNAs were reverse transcribed in situ using biotin-dCTP. The promoter lncDNAs were immunoprecipitated and pulled down with streptavidin beads. The purified lncDNAs were eluted for Illumina library sequencing. As the control, a Cas9 randomn gRNA was used in the CRIST assay. In additon, a IgG control was performed in parallel with Oct4  promoter specific Cas9 gRNAs.