title: GSM2692568: 4a: ALDH_High_DMSO_OCSA ATAC-seq; Homo sapiens; ATAC-seq
library_construction_protocol: Prior to the assay, cells were cryopreserved in culture media containing 5% DMSO. The cells were then thawed in a 37C water bath, pelleted at 500g for 5 min at 4C, washed with cold PBS, and tagmented as previously described (Buenrostro et al. 2013) with some modifications. Cell pellets were resuspended in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630), pelleted at 500g for 10 min at 4C, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure XP beads (Beckman Coulter).