title: GSM1429770: ChIP-seq Pfh1-13Myc_1; Schizosaccharomyces pombe; ChIP-Seq
library_construction_protocol: Lysates were clarified by centrifugation and chromatin was sonicated with Covaris E210 and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit TruSeq DNA sample preparation kit v2 (Illumina Cat nr. FC-121-2001). Library fragments of ~650 bp (insert plus adaptor and PCR primer sequences) were isolated from an e-gel, which corresponded to ~400 bps on the bioanalyzer. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.