title: GSM3444244: miRNA_C1; Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA was isolated from the mesangial cells using TRIzol® (Invitrogen) per the manufacturer's instructions. The samples were collected from each group (TGF-β1 and control cells) in triplicate and RNA sequencing (RNA-Seq) and miRNA sequencing (miRNA-Seq) were performed using the Illumina HiSeq™ 2000 sequencing system (BeiJing Genome Institute (BGI) Inc., Shenzhen, China). An analysis of differential RNA (DE-genes) and miRNA (DE-miRNA) expression between samples from TGF-β1-treated and control cells was then conducted. miRNA libraries were prepared for sequencing using standard Illumina protocols.