title: GSM2691970: HR10h_rice_42oC_10min_30oC_9h50min_rep1.fastq.gz; Oryza sativa; RNA-Seq
library_construction_protocol: Total RNA was extracted from each sample using the NucleoSpin RNA plant kit (Cat# 740949, Macherey-nagel). Examination of the quantity and quality of these RNA samples was determined by NanoDrop 2000 (Thermo Fisher Scientific, USA) and Bioanalyzer 2100 (Agilent Genomics, USA). Total RNA samples were sent to the Genomics Core Facility at Penn State University for the standard RNA-seq library preparation and next generation sequencing (Hiseq 2500, Illumina).