title: GSM2905790: input DNA in FEMALE WT BAT (brown adipose tissue); Mus musculus; ChIP-Seq
library_construction_protocol: Tissue was minced with curved scissors (slurry like) and put in 10ml tubes containing PBS 1% formaldehyde for crosslink for 15 min at RT and neutralized with 0.125 M glycine. Tissue was then shredded in Lysis Buffer using a Bullet Blender Homogenizer at max power for 5 min 4°C. After sonication, chromatin was incubated with 2¬μg of antibody at 4 °C overnight. Immunoprecipitated complexes were collected using sepharose beads A (Life Technologies). After extensive washes the DNA was extracted and purified by phenol/chloroform extraction. The library construction protocol followed  the manufacturer's instructions.