title: Theobroma cacao Matina 1-6 fosmid end sequencing reads
design: Fosmids were prepared and their ends sequenced according to the manufacturer on an ABI 3730xl instrument using BigDye v3.1 reagents (Applied Biosystems). Basecalling was performed with phred II (Ewing and Green 1998). Sequences were trimmed of vector and low quality bases (<phred20) with LUCY software (Chou and Holmes 2001).  Fosmids were cloned at the Clemson University Genomics Institute (CUGI) and Sanger sequencing was performed at the USDA-ARS Subtropical Horticulture Research Station (SHRS).