title: Nn_south
design: Pool of > 7 genotypes. Total RNA was DNase treated and first-strand cDNA synthesis was performed using oligo(dT) priming followed by eight PCR cycles. cDNA normalization was performed to reduce highly expressed transcripts followed by eight PCR amplification cycles. Further details can be found in Franssen et al. (2014), Marine Genomics, Genome-wide transcriptomic responses of the seagrasses Zostera marina and Nanozostera noltii under a simulated heatwave confirm functional types, DOI: 10.1016/j.margen.2014.03.004. NOTE: based on the publication Coyer et al. (2013) Systematics and Biodiversity, 11, 271–284 this species is now classified as Nanozytera noltii.