title: Leaf_transcriptome
design: Six different genotypes (2  resistant, 2 susceptible and 2 parents) were selected for RNA-seq analysis  based on phenotypic results. The growing condition of the plants, inoculum  and inoculation were performed as described above. The experiment was arranged  in three replicates and samples were collected at 10 and 12 days post  inoculation (dpi). At each time point, two leaflets were collected from each  individual plant pooled and the collected samples (totally 36) were  immediately frozen in liquid nitrogen and stored at -800C until further  processing. Total RNA was isolated from 100 mg of frozen tissue with the  RNeasy Plant Mini kit (Qiagen) including DNase treatment (RNase-free DNase  set, Qiagen) based on the manufacturers instructions. RNA quality and concentration  were estimated in ExperionTM Automated Electrophoresis System (Bio-Rad  Laboratories, Hercules, USA). cDNA library construction and sequencing of the  samples were done at the SciLifeLab (Stockholm, Sweden).