title: NextSeq 500 paired end sequencing; Targeted next-generation sequencing in family with an occurrence of neurodevelopmental disorders and epilepsy
design: Targeted next-generation sequencing in family with an occurrence of neurodevelopmental disorders and epilepsy
library_construction_protocol: Patients were diagnosed with NDD and epilepsy at the Department of Medical Genetics, University Hospital Brno, Czech Republic. Patients' and parental biological material (peripheral blood) was obtained after the signing the informed consent form (by parents) following the requirments of The Czech Society of Medical Genetics and Genomics of the Czech Medical Association of J. E. Purkyne.  Genomic DNA was extracted from the lymphocytes of the peripheral blood using the MagNaPure system (Roche Ltd, Basel, Swiss) according to manufacturers protocol. Extracted gDNA was diluted in TE buffer and stored in -20C. SureSelectXT Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library (Agilent Technologies, Santa Clara, CA, USA)