title: GSM4572226: a4 mutant treated with Ritalin – Replicate 1; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA from adult zebrafish brain were extracted by TRIzol Reagent (ambion). Add 2 brains in 1mL of TRIzol Reagent and homogenize the tissue. Add 0.2mL of chloroform per 1mL of TRIzol Reagent used for lysis. Centrifuge the sample for 15 minutes at 12,000×g at 4℃. Keep the aqueous phase containing the RNA. Add 0.5mL of isopropanol per tube and mix them. Centrifuge the sample for 5 minutes at 12,000×g at 4℃ to pellet the RNA. Wash the RNA with 75% ethanol. Centrifuge for 2 minutes at 12,000×g at 4℃ and discard the supernatant. Air dry RNA pellet for 5-10 minutes Library was performed by BGI. Briefly, the total RNA was processed with the NEBNext Ultra RNA Library Prep kit for Illumina.