title: GSM3498959: Child_low_cadmium 1747-04; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated from the buffy coat from maternal and newborn cord blood samples using PureGene reagents (Qiagen, Inc., Valencia, CA). DNA was bisulfite converted using Epitect Bisulfite kits (Qiagen, Inc., Valencia, CA) according to manufacturer's protocols. The bisulfite conversion rate was ~98.0 % across all samples. Bisulfite converted maternal DNAs were pooled and converted to sequencing libraries using TruSeq DNA Methylation kits (Illumina, Inc., San Diego, CA) according to manufacturer's protocols. Sequencing libraries from bisulfite converted newborn cord blood DNAs were prepared individually using TruSeq DNA Methylation kits, and index tagged using TruSeq DNA Methylation Index PCR Primers (Illumina, Inc.). The index tagged libraries were quantitated using the ddPCR Library Quantification Kit for Illumina TruSeq (Bio-Rad, Inc., Hercules, CA) according to manufacturer's protocols, and a QX200 Droplet Digital PCR system at the North Carolina State University Genomic Sciences Laboratory (NCSU GSL). Normalized libraries were pooled, with 4 or 5 libraries per pool. Libraries were sequenced on an Illumina HiSeq 2500 instrument at the NCSU GSL, producing 100 bp single end reads.