title: DNA-seq of Homo sapines: adult female liver
design: Libraries were generated using 10-100 ng of tissue DNA or 1.8-10 ng of plasma DNA. Library preparation, capture, and amplification were all performed according to the specifications of the Illumina TruSight Tumor 170 assay to capture DNA fragments spanning exons of 149 cancer-associated genes. DNA libraries were quantified and analyzed using a Qubit with a High Sensitivity DNA kit and a Bioanalyzer with a high-sensitivity kit, respectively. Captured DNA was sequenced as 150 bp paired-end reads using the Illumina NextSeq 500 to achieve a minimum of 30 million reads per sample.