title: GSM2905568: WT_LPS_1h_Rad21_input_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: For RAD21 ChIP cells were crosslinked (1% formaldehyde), lysed and sonicated a Bioruptor for 25 cycles at power H in RAD21 sonication buffer (1% Triton, 0.1% Sodium Deoxycholate, 0.1% SDS, 0.8M NaCl, 10mM Tris, 1mM EDTA). Then they were imunoprecipitated over-night using a-Rad21 (ab992, Abcam), incubated with Dynabeads Protein G, washed, decrosslinked and purified. Libraries were prepared using a NEBNext Ultra DNA LIbrary Prep kit (New England Biolabs E7370) and assessed for quality and quantity with Bioanalyzer and Qubit.