title: Chara braunii paired end RNA Seq: Rhizoid_01
design: Total RNA was extracted with a mirTMVana Kit (#AM1560; Thermo Fisher scientific, Waltham, MA, USA) from C. braunii s ground frozen whole plant, rhizoids and green thalli plus gametangia collected as follows. Two weeks after propagation, rhizoids grown below a metallic net were cut and flash frozen in liquid nitrogen. C. braunii thallus cleaned from any remaining rhizoids with gametangia was collected by hand under a dissecting microscope (Leica M165 FC; Leica, Wetzlar, Germany) and flash frozen in liquid nitrogen. Total RNA was DNase treated using the Turbo DNase TM Kit (#AM2238; Thermo Fisher scientific, Waltham, MA, USA) according manufacturers instructions.