title: Amplicon of Ciona intestinalis: tunic
design: Briefly, DNA was extracted with the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany). After quality controls of the extracted DNA, the V3-V4 region of the 16S rRNA gene was amplified in a one-step PCR (98 C/30 s; 30x cycles: 98 C/9 s - 55 C/30 s - 72 C/30 s; 72 C/10 min) using the primer pair 341F/806R. Amplicons of expected size (ca. 465 bp) were excised from the gel, the DNA purified via gel elution (QIAquick Gel Extraction Kit, Qiagen), normalized and pooled. Sequencing was conducted on the MiSeq platform (MiSeqFGx;  Illumina, San Diego, USA) with v3 chemistry.