title: GSM1553165: ISW1_OF_sample; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Genomic DNA was purified and heat-denatured: DNA was bound to Source 15Q resin in batch at 50mM NaOH, pH12, and eluted in NaCl, using 50mM steps (at pH 12). Fractions from 600-750 mM NaCl were collected, concentrated by precipitation. Double-stranded sequencing adaptors with N6 random overhangs ligated to each end; after second-strand synthesis using Taq, material from 200-1000bp was gel-purified and amplified by PCR. Products from 200-800 bp were gel-purified from two successive 2.5% agarose gels or using Ampure XP beads for size-selection. See extract protocol