title: GSM1432755: P2Y14-KO_pancreatic_islets_transcriptome 9; Mus musculus; RNA-Seq
library_construction_protocol: Mice were sacrificed by cervical dislocation and the pancreas was inflated by intraductal injection of ice cold DMEM media containing 0.2 mg/ml Liberase TL Research Grade. Distended pancreas was digested by shaking in a 37 °C water bath for 17 minutes. Isolated islets were washed and picked under a stereomicroscope and incubated overnight at 37°C and 5% CO2 in RPMI-1640 media containing fetal bovine serum. RNA libraries were prepared for sequencing using standard Illumina protocols TruSeq RNA Sample Preparation Kits v2