title: GSM3576124: BLO_UCMS5_S5; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were collected 14-16 hours after the last stress exposure (between ZT12.5 and ZT14.5). Brain tissues were immediately immersed in RNAlater® (Sigma-Aldrich) and stored at -20°C, while trunk blood was collected in RNAprotect Animal Blood tubes (Qiagen). Total RNA was extracted from dissected brain tissue using the RNeasy® Fibrous Tissue Mini Kit (Qiagen), and from whole blood samples using the RNeasy® Protect Animal Blood Kit (Qiagen). RNA libraries were prepared using the NEBNext® rRNA Depletion Kit and the NEBNext® Ultra Directional RNA library preparation kit with 100 ng of total RNA from each sample, according to manufacturer's protocols.