title: GSM1145412: EB_d10_shKdm5b; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvest using RNeasy and miRNeasy Qiagen kits. Double-stranded cDNA was sonicated and end-repaired using the End-It DNA End-Repair kit (Epicentre), followed by addition of a single A nucleotide, and ligation of PE adapters (Illumina) or custom indexed adapters. PCR was performed using Phusion High Fidelity PCR master mix