title: Whole Genome Sequencing of N5 (C2 Long subread)
design: Long-insert libraries were constructed using Pacific Biosciences commercially available Template Prep Kit, similarly to what has been previously described.  For each library, a sequencing primer was annealed to each SMRTbell, and the libraries bound to DNA polymerases using 3nM of the respective SMRTbell library and 3X excess DNA polymerase at a concentration of 9nM using Pacific Biosciences’ commercially available DNA/Polymerase Binding Kit 1.0, similarly to what has been previously described (D Rasko, et al. N Engl J Med 365, 709-717 (2011)).