title: Diversity assessments from microbes to mammals with eDNA metabarcoding in the Monterey Bay National Marine Sanctuar
design: This protocol is aimed at amplifying the cytochrome oxidase I gene (COI) in eukaryotes. The primers (mlCOIintF and HCO2198) utilized in this protocol are based on the primers from Folmer et al. 1994 and Leray et al. 2013.