title: Ochmacanthus_reinhardti
design: Library preparation and sequencing were performed at Arbor Biosciences (AB; arborbiosci.com; Ann Arbor, MI, USA). AB staff sheared 1-2g of DNA to 400-600 bps in length using a Diagenode Bioruptor Standard (UCD 200) with 6-8 cycles of sonication (depending on DNA quality) to prepare the libraries. The DNA libraries from 154 individuals were prepared using the Nextera (Epicentre Biotechnologies, Inc.) library preparation protocol for solution-based target enrichment following Faircloth et al. (2012) and increasing the number of PCR cycles following the tagmentation reaction to 20 as recommended by Faircloth et al (2013). AB staff used the Nextera library preparation protocol of in vitro transposition followed by PCR to prune the DNA and attach sequencing adapters (Adey et al. 2010)