title: Illumina HiSeq 2500 sequencing; Gene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana
design: Gene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana
library_construction_protocol: Approximately 30-50 seeds of each line were then placed in petri dishes on dry absorbent filter paper and left for 2 days at 4C to facilitate germination RNA was extracted using RNAqueous Small scale phenol-free total RNA isolation kit (Ambion; catalog #AM1912) according to manufacturer’s protocol RNA-seq libraries were prepared by the Max Planck Genome Center Cologne (http://mpgc.mpipz.mpg.de) according to the TruSeq RNA sample preparation v2 guide (Illumina)