title: RNA seq of F3 rat control sperm small RNA with 3 replicates
design: Prior to library preparation, total sperm RNA samples were enriched for small RNAs using the protocol provided in the Ion RNA-Seq Kit v2 (Life Technologies). Small RNA-enriched samples were used for small RNA library preparation, using the same kit, and barcoded with Ion Xpress Barcode Adapters (Life Technologies). Quality control was performed using Agilent High Sensitivity chips (Agilent). Libraries were loaded onto the same Ion PI chip via the Ion PI Template OT2 200 v3 and Ion PI Sequencing 200 v3 kits, and sequenced on an Ion Proton Sequencer (Life Technologies).