title: GSM1547539: WT-clean.txt; Oryza sativa Japonica Group; miRNA-Seq
library_construction_protocol: The fifth and sixth stage leaves of 40 days old rice were chose and tissue that 2cm within the cut site which was 4 cm from the leaf opex were collected and frozen in liquid nitrogen and used for total RNA isolation. 15 seedlings of each line at least were collected as a mix. Total RNA was isolated using TRIZOL® reagent (Invitrogen, USA) in accordance with the manufacture’s protocol for miRNA high-throughput sequencing. Small RNA sized at 18-30 nt was gel-purified on a 15% polyacrylamide denaturing gel from the total RNA for each sample. The isolated small RNAs were sequentially ligated to RNA/DNA chimeric oligonucleotide adapters, reversely transcribed, and amplified with 15 cycles of PCR to produce sequencing libraries. The Illumina sequencing was performed by Beijing Genomics Institute.