title: GSM3574298: DamWT-Tcf7l2; Mus musculus; OTHER
library_construction_protocol: Purlink genomic DNA extraction kit (Invitrogen #K182001). DamID-seq. Overnight DpnI digestion produces blunt ends, onto with an illumina PE1 sequencing adapter is ligated overnight with T4 ligase. This is followed by Nextera library construction with the following differences: only the i7 adapter is used, 4 extra cycles are used for amplification, and a higher AMPure bead concentration (1.6x) is used.