title: GSM3444274: XY4; Mus musculus; RNA-Seq
library_construction_protocol: Auxillary, brahcial, and inguinal draning lymph nodes were collected and processed into single cell suspensions. The cells were cultured in the presence of autoantigen (PLP 139-151) for 36 hours. Cells were sorted for CD4+ T cells by magnetic negative selection using EasySep Mouse CD4+ T cell isolation kit (STEMCELL). RNA was isolated from CD4+ T cells using the Direct-zol™ RNA MiniPrep Plus kit (Zymo Research). Double-stranded cDNA and the sequencing library were made using the Kapa RNA Kit Full kit.