title: WGS of Q. suber: genome survey using PacBio RS II Sequencing
design: Isolated HWM DNA was subjected to next-generation sequencing on the Pacific Biosciences (PacBio) Single Molecule, Real-Time (SMRT)DNA Sequencing System (Pacific Biosciences) as per the manufacturers protocols, at the Yale Center for Genomic Analysis (YCGA). Briefly, SMRT bell libraries were created with the Pacific Biosciences template preparation kit with its protocol for 20kb libraries. Next, SMRTbell libraries were bound to the polymerases with the DNA/polymerase binding kit and corresponding primers. Complexes containing polymerase and templates then were bound to magnetic beads and the sequencing was performed on the PacBio RS II system. Sub-read filtering was performed with the PacBio SMRT analysis software package.