title: GSM3866804: AX2 Mm_2.5hpi-A; Dictyostelium discoideum AX2; RNA-Seq
library_construction_protocol: For M. marinum infection samples, total RNA was isolated by the Ambion PureLink™ RNA Mini kit in combination with TRIzol (Invitrogen), followed by on column DNAse treatment according to the manufacturer's instructions (Ambion, Invitrogen). For L. pneumophila infection samples RNA samples were prepared by TRIzol extraction. For M. marinum infection samples, poly(A)-enriched libraries were created using TruSeq RNA Library Prep Kit (Illumina). For L. pneumophila infected samples, libraries were prepared using TruSeq stranded mRNA library preparation kit with polyA selection (Illumina).