title: GSM2905538: Rad21-/-_IfnLPS_8h_RNAseq_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from 2 millon cells per condition with RNeasy minikit and using Qiashredder disruption following the manufacturer's instructions (Qiagen). Quality of RNA was assessed using a Bioanalyzer instrument (Agilent) and quantified with Qubit (Invitrogen). ERCC RNA Spike-Ins (Ambion) were added to each purified RNA solution. 750ng of total RNA were used to prepare libraries using the TruSeq stranded total RNA Kit for Illumina following the manufacturer's recommendations. Library quality and quantity were assessed on a Bionalyser and Qubit respectively.