title: HEN1 pin/pin Round spermatids Rep 1 smallRNAseq
design: Total RNA was qualified using an Agilent BioAnalyzer and 1 µg was used for library construction with Illumina's TruSeq Small RNA sample preparation kit, according to the manufacturer's instructions (TruSeq SmallRNA SamplePrep Guide RevE). Size selection of the small RNA libraries following ligation of the adapters was done using a Pippen Prep 3% gel with an elution size range of 122-182 base pairs accounting for both miRNAs (19-22 nt) and piRNAs (26-31 nt) plus adapter sequences.