title: GSM4015492: TPR A Cyto rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Control and TPR-depleted cells were harvested, fractionated using detergent, and RNA from these fractions was isolated using TRIZOL and Ambion PureLink kit (ThermoScientific) RNA was poly(A)+ selected using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), and libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep kit (NEB, E7765S). We used NEBNext Multiplex Oligos for Illumina (Index Primers #1 - #24) (NEB, E7335S and E7500S) as the unique index primers.