title: GSM1550046: trf4D_1_T0; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA was extracted using standard hot phenol procedure. rRNAs were depleted from total RNA using the RiboMinus Eukaryote Kit for RNA-Seq (Life Technologies). Strand-specific total RNA-Seq libraries were prepared from 125 ng of rRNA-depleted RNA using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). Paired-end sequencing (2x50 nt) of the libraries was performed on a HiSeq 2500 sequencer.