title: GSM3764874: elba3_RNA-seq_rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Standard protocols were used to make chromatin extract for ChIP. Total RNAs were extracted with Trizol reagent for RNA-seq. Standard PRO-seq protocol was used to make PRO-seq libraries ChIP-seq libraries were made using NEB DNA library prep kit. ChIP-nexus library was made as previouly described (He, et al ,2015). RNA-seq libraries were made by using illumina True-seq total RNA library kit LT. PRO-seq libraries were made as previously described (Kwak, et al, 2013).