title: Fecal Microbiome Canine
design: DNA was extracted from 150 mg of lyophilized stool using the MO BIO PowerFecal DNA isolation kit (MO BIO Laboratories, Carlsbad, CA, USA) per the manufacturer's instructions. The targeted 16S small-subunit ribosomal genes were amplified using universal 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R GGACTACVSGGGTATCTAAT primers 12 via polymerase chain reaction (PCR) using a 12bp unique Golay barcoded primer for each sample. The sample amplicons were subsequently cleaned using MO BIO UltraClean-htp 96 Well PCR Clean-Up Kits (MO BIO Laboratories, Carlsbad, CA, USA), quantified using the Quant-iT PicoGreen dsDNA Reagent and Kits (Life Technologies Corporation, Grand Island, NY, USA), and pooled for sequencing. DNA extraction, amplification and cleaning was performed at the Natural Resource Ecology Laboratory at Colorado State University.