title: RNA-Seq of mus musculus adult male subchondral bone marrow B cells
design: Sequenced reads were cleaned according to a rigorous pre-processing workflow (Trimmomatic-0.32) before mapping them to the Mus musculus genome (mm10) with SHRiMP2.2.3 ( http://compbio.cs.toronto.edu/shrimp/ ). Cufflinks2.0.2 (cuffdiff2 - Running Cuffdiff) were then used to perform differential expression analysis with an FDR cutoff of 0.05 (95% confidence interval).