title: GSM2356322: sha_CT19.3; Mus musculus; RNA-Seq
library_construction_protocol: Cochlear RNAs were extracted as previously described (Patil et al, 2014) with classical methods using guanidium thiocyanate lysis buffer followed by purification on the miRNeasy Mini columns (Qiagen) following the manufacturer's protocol Total RNA was quantified with a Nanodrop and RNA integrity assessed with a Bioanalyzer (Agilent Technologies). The TruSeq mRNA stranded kit from Illumina was used for the library preparation with 300 ng of total RNA as input. Library molarity and quality was assessed with the Qubit and Tapestation using a DNA High sensitivity chip (Agilent Technologies). Pools of 6 libraries were loaded at 8.5 pM for clustering on a Single-read Illumina Flow cell. Reads of 100 bases were generated using the TruSeq SBS HS v3 chemistry on an Illumina HiSeq 2500 sequencer.