title: Polymorphism identification for sex locus mapping  (low coverage) - MRD60
design: "Genomic DNA was extracted by a modified CTAB procedure (Doyle and Doyle 1987).  Extracted DNA was sonicated to a median size of ca. 200 bp.  Samples were prepared using standard Illumina protocols with a an internal barcode index adaptor (Cronn et. al., 2008), and sequenced  in a multiplexed lane. Sequences associated with chloroplast and mitochondrial genomes were filtered out."