title: GSM3863104: HCC827 RNA-Seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from the cell lines was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's protocol. The total RNA quantity and purity were measured by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number ≥8 Approximately 10 μg of total RNA was used to deplete ribosomal RNA, was fragmented into small pieces and then was reverse-transcribed to create the final cDNA library. And then the paired-end sequencing (300±50 bp) was performed on an Illumina X10 (LC Sceiences, USA) following the vendor's recommended protocol.