title: SMRT sequencing of adult Mus musculus brain total RNA targeted RT-PCR product
design: cDNA was synthesized using the SMARTer PCR cDNA synthesis kit and amplified using Ldb3-Long-specific PCR primers. PCR products were cleaned using AMPure PB magnetic beads and quantified/sized on an Agilent 2100 Bioanalyzer. About 500 ng of each sample was used to generate SMRTbell libraries.