title: GSM2944719: Donor 1 Day 14; Homo sapiens; RNA-Seq
library_construction_protocol: On day of harvest, pellets were washed once with PBS, snap frozen in liquid nitrogen, and stored at -80ºC until processing. Isolation of RNA was performed following manufacturer's protocol (Norgen #48500) with the following modifications: for each time point, three pellets were homogenized with a pestle prior to addition of lysis buffer RL; RNA was eluted in 20 µL of DEPC-treated water. Libraries were prepared using TruSeq Stranded Total RNA with Ribo-Zero Gold kit (Illumina). Sequencing was performed on a HiSeq2500 (paired end and 100 base-pair reads) with sequencing depth of 100 million reads per samples.