title: GSM3764599: H3K27ac binding region in OS5K #2, replicate 2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP was performed using 5 μg of anti-H3K27ac antibody (#MABI0009, MCA, Nagano, Japan) or anti-BRD4 antibody (#A301-985A, Bethyl Laboratories, Montgomery, TX) as described previously (Koinuma D et al, Mol cell biol, 2009). The libraries were constructed as described (Murai et al, Cell Discov, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).