title: GSM4292837: DDX43 KH 89aa 2_ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: The DNA was extracted with phenol-chloroform, precipitated with 2×volume of ethanol, washed with 70% ethanol, and dissolved in 50 µL of ddH2O. For RNA extraction and purification, Trizol reagent (Invitrogen) was used to isolate the sheared RNA. The isolated RNA was eluted in nuclease-free water. For the DNA library, 20 ng of ChIP-ed DNA was end repaired using NEBNext Ultra II End Prep buffer and Enzyme mix, adaptor-ligated, cleaned with 0.9×AMPure beads, and size selected using 0.4×beads. The size-selected DNA was then amplified using (Index 3, 4, 5, 6, 7 and 8) NEBNext Multiplex Oligos for Illumina and universal primers (E7335, NEB). The products were cleaned using 0.9×AMPure beads. The RNA library was prepared using the NEBNext Ultra II directional library prep kit (E7760, NEB) and DNA library by NEBNext ultra II DNA library prep kit (E7645, NEB) according to the manufacturer's instructions