title: GSM4365643: wt wnt8a_7.5 rep2; Danio rerio; RNA-Seq
library_construction_protocol: hs:dkk1b/+ or hs:wnt8a/+ embryos, identified by fluorescence, and their wild-type siblings were collected from multiple mating fish pairs, then embryos at the desired time points were immediately homogenized in Trizol and RNA was extracted with Direct-zol RNA kits RNA libraries were prepared for sequencing using standard Illumina protocols