title: 16S rDNA sequencing of bacteria: Microbial community in Synechococcus co-culture
design: The bacterial hypervariable V3-V4  region of 20S rRNA gene was amplified. Sequencing libraries were constructed using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer's recommendations. The library quality wassessed with a Qubit 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system.__