title: Genome sequence update for Escherichia coli O157:H7 EDL933
design: Genomic DNA was fragmented to ~20kb. Standard PacBio library preparation was carried out thereafter. The  library was size selected in the range of 7 kb to 50 kb using a Blue Pippin (Sage Science). The library was sequenced using P5/C3 chemistry on a PacBio RS II instrument using 3 hour movies.