title: GSM3703921: Intestine 12w; Mus musculus; OTHER
library_construction_protocol: Total DNA was extracted from small intestine, liver and spleen tissues of naturally aged mice of 12 and 104 weeks of age. DNA was extracted using the Quick-DNATM Miniprep Plus Kit (Zymo Research) following the manufacturer's protocol. 500 ng of the genomic DNA was sheared on the S220 instrument (Covaris) followed by NGS library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation) and quantified using the NEBNext Library Quant Kit for Illumina (NEB). Equimolar pooled libraries were sequenced in a paired end mode (80 cycles) on the NextSeq 500 System (Illumina) using v2 chemistry yielding in an average QScore distribution of 85% >= Q30 score.