title: RNA-Seq of Mus musculus: adult male, liver, liver-specific A1cf knockout
design: Total RNA was prepared from liver pieces of central lobe through homogenization in Trizol reagent (Sigma) using a Tissue Lyser II (Qiagen). To each sample 0.2 volumes of chloroform were added, centrifuged for 15 min at 12000 g at 40 C and finally the upper aqueous phase was precipitated with isopropanol for 30 min at 20C. After resuspension in water, RNA samples were digested with 10 units of DNase I for 15 min at 37 C followed by a phenol/chloroform extraction and ethanol precipitation. The samples used for RNA sequencing were further purified using RNeasy columns (Qiagen, #74104). RNA integrity was analyzed using Agilent 2100 Bioanalyzer for all samples. For RNA-sequencing, standard methods were used. The cDNA library preparation was constructed using TruSeq mRNA library prep kit from Illumina and the sequencing was performed at the Functional Genomics Center Zurich (FGCZ) on a HiSeq 4000 platform.