title: Targeted sequencing of Plasma from patient BMBC4 using the IMPACT targeted sequencing platform
design: Targeted sequencing was performed using the Integrated Mutation Profiling of Actionable Cancer Targeted (IMPACT) platform targeting all exons of 341 cancer genes shown to harbor actionable mutations. 40-450ng of DNA was used to prepare barcoded sequence libraries (New England Biolabs, Kapa Biosystems), which were pooled at equimolar concentrations for hybridization exon capture (Nimblegen SeqCap).