title: Aerodigestive microbiome: 16S sequencing of oropharyngeal, gastric, and BAL samples
design: Samples suspended in Tris-Saline buffer were centrifuged for 3 minutes at 10,000 rcf. DNA was extracted from the sample pellet with the Qiagen DNeasy PowerSoil Kit, with the following modifications: protein precipitation in one step using 100 L of each C2 and C3 solutions, and column centrifugation at 10,000 rcf for 10 minutes.