title: WXS of the primary tumor from adult female glioblastoma
design: Genomic DNA extraction was conducted using Purelink Genomic DNA Mini kits (Thermo Fisher Scientific) to yield ample material for NGS. Whole exome sequencing was conducted using a NextSeq 550 (Illumina) sequencer using the SureSelect WES v5 chemistry (Agilent) and 125 bp paired-end, high output sequencing mode.  Samples recurrent A,  recurrent B, recurrent C,  primary tumor and matched blood yielded 256X, 241X, 218X, 271X and 111X coverage across 246M, 235M, 215M, 261M and 106M reads respectively.