title: GSM3181740: WT E12 FS RNA replicate2; Mus musculus; RNA-Seq
library_construction_protocol: E12 forestomachs were digested in 0.25% Trypsin EDTA at 37°C for 30 minutes followed by neutralization with FBS and Epcam + cells were sorted by flow cytometry. Cells were immediately used for RNA extraction. Total RNA from cells collected in TRIzol reagent (Thermo Fisher Sci.) was extracted with the RNeasy kit (Qiagen) and Dnase treatment. The SMARTer-Seq v4 Ultra Low Input RNA Kit (Clontech) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.