title: GSM3703947: GECKOv2 plasmid pool A; synthetic construct; OTHER
library_construction_protocol: Genomic DNA was prepared using the Gentra Puregene kit (Qiagen #158767) Integrated sgRNAs were amplified by PCR for 24 cycles. For sgRNA sequencing, 2 ug of gDNA per PCR reaction was used as template, and four reactions were pooled per sample to ensure sufficient representation of integrated sgRNAs. The resulting amplicon was diluted and further amplified in a second-round PCR to add Illumina adaptor sequences and sample-specific index barcodes.