title: GSM4086500: 122.1-24h; Homo sapiens; RNA-Seq
library_construction_protocol:  All human RNAs were analyzed on the Agilent Bioanalyzer (Agilent, Santa Clara, CA, USA) or the Perkin Elmer Labchip GX system (Perkin Elmer, Waltham, MA, USA) for quality assessment with RNA Integrity Number (RIN) or RNA Quality score range from 6.8–9.7 and median of 9.0.  cDNA libraries were prepared using 2 ng of total RNA and 1 L of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion®  Thermo Fisher Scientific, Waltham, MA, USA) using SMARTSeq v2 protocol  The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (Perkin Elmer, Waltham, MA, USA). Sixteen samples were subjected to an indexed PE sequencing run of 2   51 cycles on an Illumina HiSeq 4000 (16 samples/ lane), and 65 samples to an