title: GSM4572174: GC1459_rep5; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was isolated using TRIzol following the manufacturer's protocol with notable exceptions. Only 100 uL of Trizol was used, and 5 ug linear polyacrylamide (Sigma Aldrich) was included as a neutral carrier for RNA precipitation. RNA was eluted in nuclease-free water and incubated at 55C for approximately four minutes to resuspend the pellet. 140 ng of total RNA was used for each library prep. NEBNext Ultra II RNA Library Prep kit was used to perform polyA selection and prepare libraries for sequencing. The libraries were enriched with 11 cycles of PCR.