title: Alcaligenes faecalis subsp. faecalis NCIB 8687
design: A. faecalis NCIB 8687 was grown in the LB broth medium at 30oC with shaking.  Cells in late log phase growth were harvested and lysed by EDTA, lysozyme, and detergent treatment, followed by proteinase K and RNAse digestion. DNA isolation was by phenol-chloroform-isoamyl alcohol extraction and repeated isopropanol-ethanol precipitation.  DNA purity was measured as A260nm/A280nm ratio and a single DNA band of size over 20 kbp was observed after agarose gel electrophoresis.  The protocol and conditions followed those of Sambrook J., and D.W. Russell. 2001. Molecular Cloning: a laboratory manual. 3rd ed.