title: Illumina HiSeq 4000 paired end sequencing; Single cell RNAseq of embryonic stem cells at day1 and day2 of epiblast-like stem cells differentiated states
design: Single cell RNAseq of embryonic stem cells at day1 and day2 of epiblast-like stem cells differentiated states
library_construction_protocol: For ESCs, cells were maintained as described previously in NDiff 227 media  containing 2i inhibitors (CHIR99021 and PD0325901) on dishes coated with gelatin (Millipore, ES-006-B) (Yang et al., 2012). For d1EpiLCs and d2EpiLCs, cells were cultured in NDiff N2B27 media containing 12ng/ml bFGF; 20 ng/ml activin A and 1% KnockOut Serum Replacement on dishes coated with 5 μg/ml bovine plasma fibronectin (Hayashi et al., 2011). Cells have a heterozygote knock-in unstable GFP at Rex1 locus (see Yang et al 2014). not applicable ICell8 single-cell RNA-seq system (Goldstein et al, 2017)