title: GSM1429760: H3K4me3 ChIP_MDA-MB-468_control shRNA; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-seq, cells were crosslinked with formaldehyde (1% final) for 10min at room temperature, and harvested for sonication.  Nuclei were extracted and chromatin was sheared to an average size of 200bp using a Diagenode Bioruptor. Sequencing libraries for ChIP-seq were constructd using the NEBNext Ultra kit as per manufacturer's recommended instructions