title: GSM3181601: case 3 [56244]; Homo sapiens; Human betaherpesvirus 6B; RNA-Seq
library_construction_protocol: Whole blood samples were collected and frozen in Paxgene tubes. Total RNA was isolated from PAXgene tubes (MagMAX Blood RNA kit, Ambion) according to the manufacturer's instructions. The total RNA obtained was then further processed using the GLOBIN clear kit (Ambion) to strongly reduce the level of alpha and beta globin transcripts. Finally, RNA samples were further purified on “Clean and Concentrate” columns (Zymo research) according to the manufacturer's instructions. Total RNA integrity was checked using an Agilent 2200 TapeStation (Agilent Technologies) and quantified using a Trinean DropSense96 spectrophotometer (Caliper Life Sciences). For cell pellet (SupT1 T cells), total mRNA was extracted in trizol and isolated column  (Directzol, Zymoresearch) according to manufacturer's directions RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep Kit (Illumina, Inc.) and a Sciclone  NGSx Workstation (PerkinElmer).  Library size distributions were validated using an Agilent 2200 TapeStation (Agilent Technologies).  Additional library QC, blending of pooled indexed libraries, and cluster optimization was performed using Life Technology's Invitrogen Qubit® 2.0 Fluorometer (Life Technologies-Invitrogen). RNA-Seq on polyA selected mRNA