title: Illumina HiSeq 2500 paired end sequencing; RNA-Seq of Arabidopsis thaliana developing siliques and seeds with temperature shifts
design: RNA-Seq of Arabidopsis thaliana developing siliques and seeds with temperature shifts
library_construction_protocol: Total RNA is extracted using sodium tetraborate extraction, lithium chloride precipitation and cleaned up with the RNAeasy kit (Qiagen) cDNA library is prepared using TruSeq Stranded mRNA Sample Prep Kits to 25 ul minimum at 10 nM.