title: GSM4292742: input_minus; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with anti-BAP18. The steps of DNA library construction are as follows:1.Fix the ends of the DNA from the IP with base A,and repair to add A DNA add sequencing joint processing;2.Magnetic beads were used to recover the target size fragment and PCR amplification was performed to obtain the library to be sequenced;3.The libraries were examined by agarose electrophoresis;4.Qubit 2.0 was used to quantify the library to determine whether the library concentration was suitable for computer;5.After the libraries passed the quality inspection, different libraries were sequenced on Illumina sequencer according to the requirements of effective concentration and target data volume.