title: fungal ITS1 sequnce
design: The Illumina overhang adapters nucleotide sequences were added to the genespecific sequences. We generated the amplicons libraries following the Illumina Miseq sequencing protocol. The amplification experiment contained the following: 12.5 l of 2x KAPA HiFi HotStart ReadyMix, 5.0 M each of the primers listed in Table I, and 5 ul of fungal genomic DNA in 10mM Tris pH 8.5 of the mock community samples. The following PCR conditions were used: 3 min at 95 C, 30 cycles of [30s at 95 C, 30s at 55 C, 30s at 72 C], 72 C for 5 min for the final elongation. Libraries were clean up using AMPure XP beads, fresh 80% EtOH and 10 nM Tris pH 8.5. The Illumina sequencing adapters were attached to previous clean PCR product using the Nextera XT Index Kit and eight PCR cycles using the previous conditions. The products were cleaned up, denatured with NaOH diluted with hybridisation buffer and then heated before Miseq sequencing using to manufacturers instructions.