title: GSM1547549: WT +GLU noCHX fast harvest Ribo-seq; Saccharomyces cerevisiae; ncRNA-Seq
library_construction_protocol: frozen cell pellets were thawed in lysis buffer and lysis was performed with glass beads as described in the text. Extracts were clarified by a 20' spin at 11,500 rpm in an SS-34 rotor Footprints: 50 OD units of extract were treated with 50U of RNAse1 and subsequently purified from the monosome region of a sucrose gradient. RNA was extracted by hot acid phenol. Total RNA: RNA was extracted from 50 OD of extract by SDS and hot acid phenol, and poly(A) RNA was purified on DynaBeads. RNA was fragmented at 95C with zinc chloride