title: ITS rDNA ampilcon sequencing of root mycobiome:  Plantago lanceolata root
design: Before isolation of fungal endophyte DNA, roots were surface sterilized (with NaOCl). The air dried surface sterilized roots were pulverized using liquid nitrogen in two steps: first with two steel balls (2 mm) in small pieces in empty 2 ml Lysing Matrix E Tubes (MP Biomedicals) and second refilled with the corresponding lysing beads homogenized with a TissueLyser II (Qiagen GmbH, Hilden, Germany). Total DNA was extracted using the DNeasy plant mini Kit (Qiagen GmbH, Hilden, Germany). ITS rDNA amplicon libraries ITS2 region performed on the Illumina MiSeq platform (MiSeq Reagents kit v3) using the primers fITS7-ITS4 fungi.