title: Halomonhystera hermesis illumina read collection from mRNA
design: The cDNA library was constructed using the Clonetech SMARTTM cDNA Library Construction. cDNA normalisation has been performed.  Library construction and sequencing were performed by Genomics Core, UZ Leuven, Belgium. Briefly, the cDNA was sheared using the Covaris M220 machine (fragment length of 800bp). cDNA libraries were end repaired, and NEXTflexTM adaptors from BIOO Scientific were ligated using the SPRIworks robot (Beckman) followed by an AMPure bead purification to remove adaptor dimers. Libraries were amplified using a 12 cycle PCR step with NEXTflex primer mix. An additional AMPure bead purification was performed. The resulting cDNA libraries were pooled and paired-en sequenced on a single Illumina MiSeq 2*250bp lane.