title: GSM2905151: Library 2 Sorted Cells [E. coli]; Escherichia coli K-12; OTHER
library_construction_protocol: After sorting, cells were lysed with the Zymo (CN# D4036) and plasmid DNA containing PAMs was isolated Library was constructed by amplifying the diverse PAM library with conserved oligos, followed by a seconnd indexing step adding the necessary Illumina adapters.