title: GSM1910305: RLN02; Homo sapiens; RNA-Seq
library_construction_protocol: Regulatory T cells were sorted by FACS from single cell suspensions.  Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol.  RNA quality was assessed by 2100 BioAnalyzer (Agilent). Total RNA was used for cDNA synthesis using Ovation RNA-Seq (NuGEN) with normalized RNA input for each sample.  Amplified cDNA was purified through MinElute reaction cleanup column (Qiagen).  cDNA quality was assessed using 2100 BioAnalyzer (Agilent).  SOLiD protocols were followed for library prepartion for 50bp sequencing.