title: RNA-seq of Nothofagus pumilio: ZT48, 34 C, replicate 2
design: RNA extraction following Chang et al. (1993), treated with RQ1 RNAse-free DNAse (Promega), and purified with RNeasy Plant Mini Kit (Qiagen). Mature mRNA selected with Dynabeads mRNA DIRECT Micro Kit (ThermoFisher Scientific), adding ERCC RNA Spike-In Control Mix from the same manufacturer. Whole transcriptome libraries constructed with Ion Total RNA-seq Kit v2 (ionTorrent, Life Technologies), followed by emulsion PCR in an Ion OneTouch 2 System, using the Ion PI Hi-Q OT2 200 Kit (ionTorrent, Life Technologies).