title: Development of high throughput SNP-based genotyping in Acacia auriculiformis x A. mangium hybrids using short-read transcriptome data
design: First strand cDNA was synthesized using SMART cDNA synthesis kit. Second strand cDNA was synthesized using Clonetech PCR kit. The cDNA sample was purified and normalized using Evrogen TRIMMER cDNA normalization kit. The normalized cDNA sample was purified before sending to sequencing center where paired-end sequencing library was constructed following Illumina RNA-Seq protocols.