title: GSM3575562: piggc1; Mus musculus; OTHER
library_construction_protocol: Oct4GFP+ PGCs or Oct4GFP- soma from E13.5 were sorted by FACS into PGC buffer and cryopreserved in 10% DMSO. Upon thawing, samples were processed for Cut&Run analyses according to the protocol by Hainier et al.,  http://dx.doi.org/10.1101/286351, using 1000 cells per K9me2 or IgG IP on isolated nuclei. DNA was purified and eluted from a QIAGEN PCR purification kit, and the whole sample taken forward for library generation Total eluted DNA fragments following Cut&Run were used for library generation using the KAPA HyperPrep kit, using recommendations for input material <1ng, including adapter concentration of 300nM and 18 cycles of PCR. Libraries were purified with AmpureXP beads, quantified and pooled for sequencing