title: Transcriptome analysis of Nitzschia sp.
design: RNA samples from algae cultured under different conditions were extracted using TRIzol* Reagent (Invitrogen). Poly (A) RNA was purified from 5 μg pooled total RNA using oligo (dT) magnetic beads, sheared into short fragments, and primed for cDNA library synthesis using the TruSeq RNA sample preparation kit (Illumina). After quantitation using a TBS-380 mini-fluorometer (PicoGreen), the samples were clustered and sequenced on the HiSeq2000 platform (100 bp; TruSeq SBS kit v3-HS 200 cycles, Illumina).