title: GSM3498994: CG2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted with a Direct-zol RNA Mini Prep kit using the standard protocol (Zymo Research Corp., R2050) and included the treatment of the RNA with DNase I to eliminate genomic DNA. RNA concentrations and quality were determined using a Nanodrop 2000 UV-Vis Spectrophotometer (Thermo Scientific). RNA concentrations were confirmed with the Qubit RNA HS assay kit (ThermoFisher Scientific, Q32852). RNA size and integrity was measured using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Replicates for each treatment (UG, CG) were chosen for library preparation based on RNA quality.  Ribosomal RNA subtraction was done using the Ribo-Zero® Gold rRNA Removal Kit (Illumina, San Diego). Next generation sequencing libraries preparation utilized an Illumina TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego). Unique indexes were added to each of the nine samples during library construction.