title: GSM3398928: WT rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were extracted from EpiLCs 2 days after induction using RNeasy Plus Mini Kit (Qiagen). RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies). An amount of 500 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs); 2 × 36 base paired-end sequencing was performed with NextSeq500 (Illumina) by Tsukuba i-Laboratory LLP.