title: GSM4197443: rdr2-2 Anther 1; Brassica rapa; ncRNA-Seq
library_construction_protocol: Total RNA was isolated from anthers of the wild type and rdr2-2 mutant using TRIzol reagent (Invitrogen). Size selected RNA ~30 ng for each sample was used to construct the small RNA libraries using TruSeq small RNA kit from Illumina  following manufacture's protocol. Libraries were sequenced on an HiSeq2500 following the manufacturer's protocols at the University of Delaware DNA Sequencing & Genotyping Center.