title: GSM3872507: Control Diet #02; Mus musculus; RNA-Seq
library_construction_protocol: medial prefrontal cortex was fresh dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent and MagMAX-96 Total RNA Isolation Kit . DNA contamination removed using DNA-free kit.  (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA was fragmented then first strand synthesis occurred using majority deoxycytidine nucleotides which served as an anchor for the switch oligos. swOligo used: /5Me-isodC/iisodG/iMeisodC/CCATGCGGCTACACGACGC TCTTCCGATCTNNMWrGrGrG. cDNA strand with portions of adapter were added then amplified, followed by second PCR to add index portion of the adapter. Samples were then pooled, size selected with Pippin then sequenced on a Single End Run using the HiSeq 2500 for a read depth of ~4 million reads per sample