title: Fungi in wild rice  in low nutrient soil in Guangdong
design: Soil DNA was extracted from 1 g of rhizospheric soil from each sample with a FastDNATM SPIN Kit (MP Biomedicals, CA, USA) . Partial  ITS amplicons were produced for each sample using barcoded primers. The primer pair used to amplify the fungal ITS region was ITS3F (5'-GATGAAGAACGYAGYRAA-3') and ITS4R (5'-TCCTCCGCTTATTGATATGC-3')). The PCR program was as follows: 2 min at 95C; 25 cycles of 30 s at 95C, 30 s at 55C, and 45 s at 72C; and a final extension step at 72C for 10 min. The PCR products were purified using a Qiagen PCR Purification Kit (Qiagen, Inc., Shanghai, China) and pooled in equimolar concentrations.