title: Whole genome sequencing of Streptococcus thermophilus
design: 1.6 ug of Qubit quantified DNA was sonicated for 300 sec on Covaris. 950 ng of DNA was taken for library prep. End-repair and adapter ligation was done according to the protocol (Ion plus fragment library kit, (# 4471252)). The sample was barcoded at this step. The sample was cleaned using Ampure XP beads. Sample was size selected using 2% Low melting agarose gel. The gel purified sample was amplified as per the protocol. The amplified product was cleaned up using Ampure XP beads. The sample was run on Bioanalyzer HS chip to check the distribution and was quantified using Qubit Fluorometer.