title: GSM3184671: NFKB1KO_TAM; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using mRNeasy Mini Kit following the manufacturer's instruction. We performed RNA-seq experiments for cells before cell transformation, and cells after 24 hrs of cell transformation. For cells with gene knockouts, we performed RNA-seq for cells after 24hrs of cell transformation. RNA-seq libraries were prepared using TruSeq Ribo Profile Mammalian Kit (Illumina, RPHMR12126) as per manufacturer's instruction. RNA-seq libraries were sequenced by Harvard Bauer Core Facility using Hiseq 2000.