title: GSM1430351: OS3_HpaII; Homo sapiens; MRE-Seq
library_construction_protocol: Cell lysis with proteinase K and RNAse A.  DNA concentration either via ethanol precipitation or dialysis with SSC. HELP tagging: 500 ng of genomic DNA digested with either MspI or HpaII.  Custom Illumina adapter also containing an EcoP15I restriction site and a T7 transcription promoter was ligated to both ends.  Following digestion with EcoP15I, end repair, and dA tailing a second Illumina adapter containing the Illumina sequencing primer, which also contains the multiplex barcode was added.  After transcription, reverse transcription, and PCR, the libraries were gel extracted and sequenced.