title: Myrna: leaves (2012) - replicate 1
design: 500 mg of leaves at commercial maturation stage were used to isolate total RNA by TRIzol reagent (Invitrogen); yields were estimated by electrophoretic and spectrophotometric analyses (NanoDrop ND-1000; Thermo Scientific Inc.), and RNA integrity number (RIN>7) was verified using BioAnalyzer 2100 (Agilent Technologies Inc). cDNA libraries were prepared using TruSeq RNA-seq sample preparation kit (Illumina) and sequenced in 100bp paired-end mode using an Illumina HiSeq2000 (IGA Technology Services, Udine, Italy).