title: GSM4365689: Col0 rep2 (RNAseq); Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA samples were prepared from three-week old leaf tissue using the RNeasy Plant Mini Kit (Qiagen). RNA samples were prepared from three-week old leaf tissue using the RNeasy Plant Mini Kit (Qiagen). RNA and ChIP sequencing libraries were prepared at the Yale Center for Genome Analysis (YCGA). RNA samples were quantified and checked for quality using the Agilent 2100 Bioanalyzer Nano RNA Assay. Library preparation was performed using Illumina's TruSeq Stranded Total RNA with Ribo-Zero Plant in which samples were normalized with a total RNA input of 1 mg and library amplification with 8 PCR cycles.