title: GSM4575880: wild type  d0 [R0]; Mus musculus; RNA-Seq
library_construction_protocol: Cells from a 6cm culture dish were harvested by re-suspension in 500mL TRIzol (Invitrogen), and total RNA was extracted with the TRIzol method following the manufacturer's protocol. RNA was quality controlled by the nanodrop and bioanalyzer. High-quality RNA (RIN > 9 and 260/230 > 2; 260/280 > 1.8) was converted to cDNA. RNA-Seq libraries were prepared with NUGEN Ovation and Encore kits according to manufacturer's protocols