title: GSM1547562: Trophs_H3K4me3; Plasmodium falciparum; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and antibody-DNA complexes were isolated with antibody. ChIP-seq libraries for all the samples were prepared from around 5 ng of DNA using fragment library construction kit from life technolofies for SoLiD sequencing. Briefly ChIPed DNA samples were end repaired and adapters were ligated using T4 DNA ligase. Ligated DNA were subsequently amplified using adapter specific barcoded primers for 15 cycles. DNA purification at every step was performed using Agencourt XP beads. Library profiles were assessed using Agilent bioanalyzer 2100 high sensitivityDNA kit. Equimolar amount of libraries were pooled and 50 bp reads were sequenced in-house using SoLiD 4.0.