title: GSM1145845: Control siRNA, No shear stress.; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was purified using trizol extraction followed by RNeasy MinElute Cleanup Kit with on column DNAse digestion (Qiagen, Valencia, CA). Biological triplicates were pooled prior to library generation. 50 ng of RNA was used with the Ovation® RNA-Seq System (7100-08)(NuGEN, San Carlos, CA) and manufacturer’s protocol to synthesize and SPIA amplify cDNA, which was then sheared with a covaris S-series. After end repair, purification and P1 P2 adaptor ligation, templated beads were generated with the EZ beads system, and libraries where then paired end sequened (50bp forward, 35bp reverse) on a SOLiD4 DNA sequencing system (Life Technologies).