title: GSM3863113: LSF_ChIPseq [LSF+ Input_di-nucleosome]; Homo sapiens; ChIP-Seq
library_construction_protocol: The cells harvested following the ChIP procedure using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology). Chromatin was immunoprecipitated with HA antibody (Abcam 9110) and was captured by Protein A magnetic beads. Cross-links were reversed, and DNA was purified, separating the mono- versus di-nucleosome sized DNA fragments. 50 ng of ChIP-ed DNA were used for the DNA library preparation using the NEBNext Ultra II DNA library Prep Kit for Illumina (NEB E76745S).