title: GSM4085074: DMSO pSer2 Pol II ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: CAL-62 cells were fixed with 1% formaldehyde for 10 minutes at room temperature (RT). Cross-linking was stopped with 150 mM glycine. Cells were harvested and sonicated to obtain chromatin fragments (100–500 bp) with SONICS Vibra-cellTM. Library construction were performed using VAHTS Universal DNA Library Prep Kit (Vazyme, ND607-02) and VATHS DNA Adapters set2 for Illumina (Vazyme Biotech, Cat.N802-01)