title: GSM2916063: Full 1; Homo sapiens; RNA-Seq
library_construction_protocol: 36 h later, RNA was harvested with a Qiagen RNeasy Plus kit after homogenization of cells with a QIAshredder. The experiment was performed in biological triplicate. Total RNA was submitted to the MIT BioMicroCenter for library preparation and sequencing. RNA integrity and concentration were checked on a Fragment Analyzer (Advanced Analytical). The mRNA was purified by polyA-tail enrichment, fragmented, and reverse transcribed into cDNA (Illumina TruSeq). cDNA samples were then end-repaired and adaptor-ligated using the SPRI-works Fragment Library System I (Beckman Coulter Genomics) and indexed during amplification. Libraries were quantified using the Fragment Analyzer (Advanced Analytical) and qPCR before being loaded for single-end sequencing using the Illumina HiSeq 2000.