title: GSM2909661: P1072015H08 [saliva RNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-Seq, total RNA was extracted using the Qiagen RNeasy kit (Qiagen) and treated with RNAse-free DNase (Qiagen). RNA integrity was checked on a Bioanalyzer 2100 (Agilent, Santa Clara CA) Libraries were constructed by Novogene, using a Eukaryotic directional  mRNA  library (NEB).  cDNA preliminary concentration was quantitated on a Qubit (Life Technologies), an Agilent Bioanalyzer 2100 was used to test the insert size, and Q-PCR was used to quantify the library effective concentration precisely.