title: GSM3330975: InsituHiC_IMR90_RS_Bio1; Homo sapiens; Hi-C
library_construction_protocol: In situ Hi-C was performed as previously described (Rao et al., 2014, Cell) Sequencing adapters were ligated using NEBNext Ultra Ligation module (New England Biolabs, Inc) on Dynabeads, and DNA was PCR-amplified for 8-14 cycles using NEBNext Q5 Hot Start HiFi PCR master Mix and NEBNext multiplex oligos (New England Biolabs, Inc).