title: mESC_holo_sc4
design: A single cell was lysed with 100 ng of the carrier RNA mixture. The                 sequencing library was constructed following the manufacturer's protocol using                 NEBNext kits (E7490 & E7530). After adaptor ligation and USER digestion, cDNA                 fragments from the RNA carrier were removed by Not I digestion. After Not I                 digestion, the library DNA was purified for PCR amplification.