title: GSM3030647: WT 24 hr PCS rep1; Mus musculus; RNA-Seq
library_construction_protocol: By using in vivo mouse model of cataract surgery, lens fiber cells were removed from wild type (WT) via a central corneal incision, leaving behind the lens capsule and attached LECs, samples were harvested at 0 hour and 24 hours PCS, flash frozen on dry ice, and RNA was harvested using RNeasy Mini Kit (50) from Qiagen (Cat No./ID: 74104). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols for Stradeded TruSeq Library Preparation Kit for the Illumina HiSeq 2500