title: HiSeq sequencing of bacteria in substrate of Ganoderma lucidum
design: 16S rRNA distinct regions were amplified used specific primer with the barcode. Sequencing libraries were generated usingTruSeq? DNA PCR-Free Sample Preparation Kit, and then thelibrary was sequenced on an Illumina HiSeq 2500 platform and 250 bp paired-end reads were generated.