title: GSM3763336: vOrganoids_d100; Homo sapiens; RNA-Seq
library_construction_protocol: The organoids with and without HUVECs at 65 days and 100 days, respectively, were cut into small pieces and dissociated into single-cell suspensions by using a papain-based dissociation protocol (hibernate E medium with 1 mg/ml papain (Sigma) at 37℃ on a thermo cycler with 500g for 15-20 min). Single cells were suspended in 0.04% BSA/PBS at the proper concentration to generate cDNA libraries with Single Cell 3' Reagent Kits, according to the manufacturer's protocol. Briefly, after the cDNA amplification, enzymatic fragmentation and size selection were performed to optimize the cDNA size. P5, P7, an index sample, and R2 (read 2 primer sequence) were added to each selected cDNA during end repair and adaptor ligation. P5 and P7 primers were used in Illumina bridge amplification of the cDNA (http://10xgenomics.com). Finally, the library was processed on the Illumina platform for sequencing with 150bp pair-end reads. Illumina HiSeq4000