title: GSM3573163: EL4_ATAC_seq_rep2; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq was performed as previously described with minor modifications (Buenrostro et al., Nature Methods, 2013). Fifty thousand cells were pelleted at 550 x g and washed with 1 mL 1x PBS followed by lysis with 50ul lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Accessible chromatin was tagmented with Tn5 transposase (FC-121-1030; Illumina) for 30 minutes in a 37°C waterbath. Tagmented DNA was amplified for 12 cycles of PCR, purified, paired end sequencing (38 bp+37 bp) was performed on a NextSeq 550 following the manufacturer's protocol.