title: Illumina HiSeq 2500 paired end sequencing; Examination of mRNA decay rate in mature adipocytes
design: Examination of mRNA decay rate in mature adipocytes
library_construction_protocol: Primary adipocytes were cultured in DMEM/F-12 containing 10% FBS. They were treated with 2.5 µg/mL actinomycin D for 0, 4, or 8 hours. Isogen II extraction of total RNA was performed according to the manufacturer's instructions. 1 µg of total RNA was used for RNA-seq library preparation with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina), according to the manufacturer's protocol.