title: sparse seqeuncing of Arabdiopsis thaliana mapping population Col-0 x WS-2 : recq4a: Sample 271
design: "Sequencing fragments were created using restriction enzyme. After digestion the fragments were A-tailed and ligated to adaptor sequence, (barcoded version of Illumina P1 adapter and standard Illumina P2). The resulting barcoded fragments were pooled, tested for even representation, size distribution and finalized for sequencing. More detail information can be found in the method part of the paper ""Rapid and inexpensive whole-genome genotyping-by-sequencing for crossover localization and fine-scale genetic mapping"""