title: GSM3576156: L3_salivary_gland_H3K9R_rep1; Drosophila melanogaster; OTHER
library_construction_protocol: Salivary glands from female, wandering third-instar larvae were isolated and flash frozen in liquid N2 until all samples were collected. Nuclei were isolated from replicates of 25 salivary glands and sonicated with a Branson Sonifier 450 to an average fragment size distribution of 500-1000bp. Sonicated samples were treated with 100 µg/mL RNaseA at 37°C for 1 hour and with 200 µg/mL proteinase K at 37°C for 2 hours. Genomic DNA was phenol chloroform extracted and stored at -80°C prior to library preparation. Libraries were prepared from 20 ng of genomic DNA with the ThruPLEX DNA-seq kit (Rubicon Genomics) and sequenced on an Illumina HiSeq2500 at the UNC High-Throughput Sequencing Core Facility.