title: We assembled, annotated and analyzed the complete chloroplast sequence of sweet potato (Ipomoea batatas)
design: Young leaves of plants were collected, snap-frozen immediately in nitrogen and stored at -80 °C until further processing. DNA extraction and purification were performed using the CTAB method. Agarose gel electrophoresis was used to show the integrity of the DNA, while spectrophotometry was employed to display the concentration and cleanliness. For the Illumina HiSeq 2000 (GA II) platform, library construction and sequencing was provided at Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, China (http://www.genomics.cn). Sequencing libraries included paired-end library of 500 bp insert and mate-pair libraries with 2 kb insert sizes were constructed, resulting in 20G raw data of 90 bp sequenced reads. One lane of the flow cell was used for each sequencing library.