title: F120-1
design: Single colony of strain F120-1 was precultured overnight with 3 mL LB medium in a rotary shak-er (200 rpm) at 37 . Then 1 mL of preculture was inoculated into 100 mL M9Y medium and anaerobiclly cultivated at the same conditions for 12 h, with E.coli W3110 served as a control. Each sample has three replicates. Total RNA was extracted with RNeasy Mini Kit (QIAGEN GmbH, Germany), then on column digestion with DNAse (QIAGEN GmbH, Germany) was per-formed to remove genomic DNA contamination. Total RNA samples were analyzed with Agilent 2100 Bioanalyzer (Agilent Technologies, inc., US) before library construction for RNA-Seq analysis. After that, mRNA was purified with Ambion MICROBExpressTM Bacterial mRNA Enrichment Kit (Life Technologies Corporation, US)and cut into short fragments. These short mRNA fragments were served as templates for cDNA synthesis. The first-strand cDNA was synthesized with random hexamer primers, RNA templates and Reverse transcriptase, while the second-strand cDNA was synthesized with RNase H and DNA polymerase I. Then short DNA fragments were purified with QIAQuick PCR Purification kit (QIAGEN GmbH, Germany) and prepared for end repairing and poly (A) ligation. Sequencing adaptors were then ligated to the refined short fragments and prepared for PCR amplification. The libraries constructed were then loaded for sequencing with the HiseqTM 2000 Sequencing System (Illumina, Inc., US).