title: RNA-Seq of Salicornia persica : shoot
design: Total RNAs were isolated from sampled shoot tissues of S. persica treated with 0 and 300 mM NaCl solution using column RNA isolation kit (QIAGEN RNeasy Plant Mini kit, USA) according to the manufactures protocol. RNA quantity and integrity were checked using agarose gel electrophoresis, Nanodrop ND-1000 (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer. DNase I treatment was also applied to ensure removing genomic DNA contamination from RNA samples. High quality RNA samples were used to construct cDNA libraries followed by paired-end high-throughput sequencing using Illumina HiSeqTM 2500 platform by the Macrogen Company (Korea).