title: GSM3374322: S243; Mus musculus; RNA-Seq
library_construction_protocol: RNEasy mini kit (QIAGEN) on QIACUBE (QIAGEN) RNA was isolated on a Qiacube using the RNeasy mini kit (Qiagen) and treated with Dnase to remove contaminating DNA. RNA quality was confirmed using a BioAnalyzer (Agilent Technologies). All samples had RNA Integrity Numbers (RIN values) > 8.0. Sequencing libraries were prepared from 1 µg RNA with the Truseq Stranded mRNA Sample Preparation Kit (Illumina Inc) following the manufacturer's protocol. Final PCR-enriched fragments were validated on a 2200 Tapestation Instrument using the D1000 ScreenTape (Agilent Technologies) and quantified by qPCR using a Universal Library Quantification Kit (Kapa Biosystems) on the QuantStudio 6 Flex (ThermoFisher Scientific). Final library pools were sequenced by 75bp paired-end sequencing on a HiSeq2500 (Illumina Inc). 