title: Illumina HiSeq 2500 paired end sequencing; Overexpression of RORA in primary mouse in vitro Th2
design: Overexpression of RORA in primary mouse in vitro Th2
library_construction_protocol: Naive CD4+ T cells were extracted from JAXJ mouse spleens using Miltenyi naive T cell purification kit. On day 4, cells were FACS sorted and mCherry/GFP+ cells were kept for RNAseq Cells were grown and transduced with retrovirus following (Henriksson, Xi, et al, Cell, 2019). RNA was extracted using RNeasy mini kit columns RNA was processed by the Sanger pipelines to generate cDNA (truseq)