title: Anchored phylogenomics of leech-like annelids: Piscicola geometra
design: After quantification using a Qubit fluorometer, DNA extracts were sonicated using a Covaris ultrasonicator to a fragment size of 150-500bp. From this DNA, indexed libraries were prepared following Lemmon et al. 2012 (Syst. Biol. 61, 727744) and Prum et al. 2015 (Nature 526, 569573), using a Beckman Coulter FXp liquid-handling robot. Libraries were then Qubit-quantified and pooled in two pools of approximately 12 samples for enrichment. The probe set Ann1a was produced by Agilent and used to enrich the libraries. Enriched libraries were quantified using KAPA qPCR, then sequenced on one Illumina HiSeq2500 lane with a paired-end 150bp protocol with 8bp indexing.