title: GSM1552789: SF3B1 mutated sample 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent, DNase treated (Invitrogen) and purified using XP beads (Beckman Coulter). Purified DNase-treated total RNA (100ng) was processed using NEBNext Ultra directional mRNA Library prep (NEB) as per manufacturer’s protocol for Illumina with two alterations: custom indexes were designed in-house and an additional purification step was performed using XP beads (Beckman Coulter) rather than size selection.