title: GSM3498208: shSmc1a E14 RNA-Seq 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted for each of the transfected cells using Trizol reagent (Ambion). DNA contamination for the samples was minimized by using the QIAGEN RNeasy Kit. The RNA sample quality control was performed by qPCR for constitutive genes (Actin, Gapdh) as well as by determination of knock-down efficiency. TruSeq Stranded mRNA Library Prep Kit (RS-122-2101, Illumina) was used and the libraries were prepared as per the recommended kit protocol.