title: DNA-seq of Phialemoniopsis curvata:culture
design: Libraries prepared using Nextera DNA Flex preparation; Tagmented (tagged and fragmented) DNA linked to beads and PCR amplified with unique barcode primers; PCR products purified with Ampure beads and Nextera library preparations quantified prior to multiplexing and sequencing in a paired-end 300 cycle mode on Illumina MiSeq using 600-cycle reagent kit v.3