title: GSM3764474: Heart, control, RT3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from frozen LV tissue using the RNeasy Fibrous Tissue Mini Kit (Qiagen) according to the manufacturer's instructions. RNA samples (RNA integrity number (RIN) >8) were submitted to Northwest Genome Center for library preparation and sequencing. Poly(A)- enriched cDNA libraries were prepared using the Illumina TruSeq RNA Library Preparation kit according to the manufacturer's instructions. Samples were run on Illumina HiSeq 2000 at a read length of 100nt single end, and a depth of about 50 million per sample. Five mice per genotype or diet condition are used for subsequent analysis Poly(A)- enriched cDNA libraries were prepared using the Illumina TruSeq RNA Library Preparation kit according to the manufacturer's instructions