title: GBS-seq of zea mays:mature leaf
design: Firstly, the DNA to be sequenced and the splices with different barcode are placed on the plate, and the DNA is digested by apek I and hind III. Secondly, the splices are connected to the sticky ends of the fragments due to restriction endonuclease digestion by T111 ligase, and then the DNA mixing pool with different barcode is mixed. Thirdly, the unreacted splices are filtered out by fragment length screening column. Finally, as the primers are added into it, the amplified products were sequenced by HiSeq X Ten instrument.