title: GSM3576167: 7.036; Homo sapiens; RNA-Seq
library_construction_protocol: Approximately 20, 5-mm sections of FFPE tissue from each subject were anonymized and stained with H&E immediately before the stroma were microdissected by a pathologist using a dissecting microscope. RNA was purified using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion) and amplified by the WT-Ovation FFPE System (NuGEN Technologies) as per the manufacturers' protocols. RNA was quantified with NanoDrop ND-1000 followed by quality assessment with 2100 Bioanalyzer (Agilent Technologies) according to manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols