title: GSM3864962: Zygote Col0xTsu1 biological rep 1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Embryos were manually isolated as in Xiang et al., 2011. Embryos were washed by moving them away form the maternal tissues to a corner of the petri dish and then pipetted along with <5uL intro another petri dish containing 50 mL of the isolating solution. RNA was extracted using the RNAqueous-Micro kit (Ambion, Catalog# 1927). The mRNA amplification was conducted according to the protocol provided in the MessageAmp aRNA kit (Ambion, Catalog# 1750). Illumina mRNA-seq libraries were prepared using the TruSeq RNA kit (Ver 1, rev A).