title: GSM1146788: H3K27me3_ChIPSeq in brm rep2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei, and histone-DNA complexes were isolated with H3K27me3 antibody (07-449, Millipore). Ten ng of ChIP DNA immuoprecipitated by anti-H3K27me3 antibody was used for ChIP-seq library construction. Blunting end, adapter ligation and amplification were carried out using Illumina Genomic DNA Sample Prep Kits according to the manufacturer's protocol. Illumina Genome Analyser IIx was used for high-throughput sequencing of the ChIP-seq library.