title: GSM3869871: F518_M145_37h; Streptomyces coelicolor A3(2); RNA-Seq
library_construction_protocol: Lysates were extracted using RNAprotect Bacteria (Qiagen) kit and RNeasy mini kit (Qiagen) Libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) following the instructions according to NED. Breifly, 1µg of RNA was subject to rRNA depletion using Ribo-Zero rRNA Removal Kit Bacteria (Illumina). The resulting tRNA was used to construct the cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB).