title: GSM3573484: 5P-WT_dmso-rep2; Saccharomyces cerevisiae BY4741; RNA-Seq
library_construction_protocol: RNA was extracted using a standard hot acidic phenol approach and treated with DNase I (Ambion, #AM1906). As starting material, 50 ug of RNA was used. The Ribo-Zero Magnetic Gold Kit (Epicentre) was used to deplete rRNA, and libraries were generated as previously described (PMID: 23295673). Libraries were sequenced (single-end, 100 bp) using an Illumina HiSeq 2000. Reads contain a unique molecular identifier (UMI) that is 8 nucleotides long followed by the 5' sequence. RNA fragments shorter than 100 bp may container adapter sequences that may be trimmed (AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC).