title: RNA-Seq of Mugil incilis: Juvenile Liver
design: Liver sample was frozen in liquid nitrogen and ground to a fine powder in a pre-cooled mortar and pestle, extractions were performed according to the manufacturer's directions using Trizol reagent. RNA concentration and integrity of the sample was measured on an Agilent 2100 Bioanalyzer (Agilent Technologies) using an RNA Nano Bioanalysis chip. mRNA enrichment, library construction,  and sequencing was conducted by Macrogen Inc. with Truseq mRNA kits and the Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads.