title: GSM3399474: RNA_RA_rep2; Mus musculus; RNA-Seq
library_construction_protocol: 2x105 low passage (< 10) E14 cells were plated per 10 cm culture dish and cultivated for 48 h in regular ES medium containing LIF. 4 h prior to harvest, medium was exchanged and cells were treated with LIF or RA as described in the treatment protocol. Cells were harvested and subjected to RNA extraction applying RNeasy Mini Kit (Qiagen) according to the manufacturers' instructions. The experiment was performed in biological triplicates. Sequencing libraries were generated using TruSeq® Stranded mRNA Kit (Illumina) according to manufacturer's instructions.