title: GSM4086234: C6 AQP1 rep2; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol (Invitrogene, Lot# 15596-018) and genomic DNA contamination removed with RNase-free DNase I (Transgen, Code#GD201-01) following the manufacturer's protocols. RNA degradation and contamination were monitored on 1% agarose gels. RNA was reverse-transcribed into cDNA using the M-MLV Reverse Transcriptase (Promega, Lot# M5301). 10ng of total RNA were reverse transcribed and amplified using the Ovation RNA-seq System V2 (Nugen). Libraries were constructed from 100ng of sonicated cDNA (Covaris) using the Ovation Ultra Low DR Multiplex system (Nugen). The concentration and quality of the libraries were determined using Qubit (Invitrogen) and Bioanalyzer (Agilent). Constructed RNA-seq libraries were sequenced on the Illumina HiSeq 2500 platform, obtaining 100-nucleotide single reads.