title: Through 16s sequencing data analyse swine manure and housefly larvae's gut microbiome
design: In this experiment we have 48 samples, in order to reduce the cost we sequenced these samples' 16S rDNA V4 region(primers are 515F and 806R), but make only one sequencing library. Through PCR, we have added barcode sequences ahead the reverser primer, then we can demultiplex samples with different barcodes in every sample when we analyse these data. The library Construction we refer the Earth Microbiome Project's 16S rRNA amplification protocal http://www.earthmicrobiome.org/emp-standard-protocols/16s/. Our samples information you can refer our paper when it was published.