title: RNAseq analysis of Tg(Ctsl-Gdnf) and Tg(Ctsl-Gdnf):luxoid Testes
design: Gene expression profile of  4 month old Tg WT and Tg luxoid (plzf lu/lu) mutant testes by deep sequencing. The libraries were pooled and loaded onto 3 lanes of  a V3 Flow cell and sequenced on the Illumina HiSeq 2000 (100bp paired-end run). RNAseq data were subjected to quality control check by NGSQCtoolkit v 2and samples with base qualities >30 over 70bp were used in the analysis. The libraries were pooled and loaded onto 3 lanes of  a V3 Flow cell and sequenced on the Illumina HiSeq 2000 (100bp paired-end run). RNAseq data were subjected to quality control check by NGSQCtoolkit v 2and samples with base qualities >30 over 70bp were used in the analysis. 96% of 120 million reads were mapped to mouse genome (build mm9) of which 75% were unique, with median insert ~280bp.