title: 454 GS FLX Titanium sequencing
design: Bavarian Cave with former medicinal spring, microbiology: 16S rRNA gene sequencing of thick biofilms and snottites on ceiling and walls and spring water, geochemical: methane and iodine
library_construction_protocol: Biofilm and water samples extracted and 16S rRNA amplified with 27f and 519r barcoded primers, Sequencing using GS Flx Titanium (454)