title: ChIP-seq high LP in Neurospora crassa
design: Cultures were grown 36 hours in light and transferred to constant darkness for 24 hours. 1 minute high LP is given and cultures were crosslinked and harvested 0, 1, 5, 10, 30, 60, 90, 120 min after LP. Ground mycelia was sonicated using a Covaris S220X sonicator (180 peak power, 20.0 duty factor, 200 cycles/bursts, for 4 min at 4°C). Total extracts were subjected to ChIP with specific affinity purified antibodies for RNA polymerase II. ChIP DNA libraries were prepared with NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® with NEBNext® Multiplex oligos.