title: WGS of Environmental DNA from paper mill waste: Primary_wastewater_sludge (paired-end_reads and assmebly)
design: DNA library preparations and sequencing reactions were conducted at GENEWIZ, Inc.(NEB NextUltra DNA Library Preparation kit was used following the manufacturer s recommendations; the genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument.  The DNA was end repaired and adenylated.  Adapters were ligated after adenylation of the 3 ends.  Adapter-ligated DNA was indexed and enriched by limited cycle PCR. The DNA library was validated using TapeStation and was quantified using Qubit 2.0 Fluorometer. The sequencing libraries will be multiplexed and clustered on one lane of a flowcell. After clustering, the flowcell will be loaded on the Illumina HiSeq instrument according to manufacturer s instructions.  The samples will be sequenced using a 2x150 Paired End (PE) configuration.  Image analysis and base calling were conducted by the HiSeq Control Software (HCS) on the HiSeq instrument.  Raw sequence data (.bcl files) generated from Illumina HiSeq were converted into fastq files and de-multiplexed using Illumina bsl2fastq v. 2.17 program. One mis-match was allowed for index sequence identification.