title: GSM3330205: Degraded_HEK_RNA; Homo sapiens; miRNA-Seq
library_construction_protocol: Trizol Libraries were prepared based on Illumina TruSeq small RNA kit v2. 1 μg of HEK-293T and Drosophila S2 total RNA was used for standard small RNA library preparation. Contaminated HEK-293T small RNA libraries were generated by pre-mixing HEK-293T total RNA with the total RNA isolated from S2 cells. The following ratios of HEK-293T to S2 total RNA were used 10:1, 100:1, 1000:1. 50 ng of HEK-293T total RNA were used for preparation of low input small RNA library. 1 μg of RNase A treated total RNA for 5 min at room temperature was used for preparation of degraded small RNA library. Single-end sequencing was carried out on an Illumina NextSeq 500.