title: GSM1550732: dme_maternal; Drosophila melanogaster; miRNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol reagent (Life Technologies), following instructions given by the manufacturer, and dissolving the RNA in RNase-free water. cDNA libraries were generated with TruSeq Small RNA Sample Preparation Kit (Illumina) following specifications from the manual. Amplified cDNA constructs were size selected in a 6% PAGE gel for 145 to 160 bp (fragments including RNA-derived sequences of size ~20-30 bp plus adapters). Size selected cDNAs were purified and precipitated with ethanol, and DNA integrity was checked with TapeStation (Agilent).