title: GSM3444213: EGF_S02; Homo sapiens; RNA-Seq
library_construction_protocol: After 24hrs, media was aspirated and total cellular RNA was extracted following the manufacturer's guidelines using a Nucleospin RNA II kit from Macherey-Nagel having pooled cells from two wells for each RNA column to increase yield. RNA yield was quantified and checked for quality using a Nanodrop spectrophotometer before quality control and further quantification using an Agilent bioanalyser prior to RNAseq