title: Enrichment of polymorphisms via hybridization for mapping of a sex locus - MRD30x60.6
design: Genomic DNA was extracted by a modified CTAB procedure (Doyle and Doyle 1987).  Extracted DNA was sonicated to a median size of ca. 200 bp.  Samples were prepared for multiplex sequencing using external index adaptors following standard Illumina protocols.  Libraries were subjected to solution hybridization using a MycroArray MyBait protocol with RNA baits designed to target previously identified polymorphic locations.