title: PARE-seq of oryza sativa japonica group: immature panicle membrane_bound polysome
design: After membrane-bound polysome isolation by ultracentrifugation, total RNAs were extracted from the membrane-bound polysomes of rice (Oryza sative japonica group, cv. Nipponbare) immature panicles with TRI Reagent (Molecular Research Center, USA) and used for PARE (parallel analysis of RNA end) library construction according to the method describe by Zhai et al. [Zhai J, Arikit S, Simon SA, Kingham BF, Meyers BC. Rapid construction of parallel analysis of RNA end (PARE) libraries for Illumina sequencing. Methods. 2014;67:8490.].