title: Microbial 16S rRNA gene amplicon sequencing: mouse stool samples
design: Bacterial DNA was extracted using the Power Soil DNA Isolation Kit (MoBio). 16S rRNA gene amplicon sequencing was performed on an Illumina MiSeq at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory and used to generate 151bp paired-end reads from the fecal samples with 12 bp barcodes. The V4 region of the 16S rRNA gene (515F-806R) was PCR amplified with region-specific primers that include sequencer adapter sequences used in the Illumina flowcell.