title: GSM3181767: ICb-1078MB-22241-digoxin-bio_S13_L003; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from tumor cells using TRIzol reagent and mRNAs were selected using Poly (A) Purist MAG kit (Thermofisher).  The RNA library prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England lab, NEB) and sequenced using NextSeq 500/550 v2 Kits (high output 150 cycle, Illumina) on a HiSeq 2500 system. RNA libraries were prepared for sequencing using standard Illumina protocols