title: RNA-Seq of Ciona intestinalis : Whole Embryo WT rep2 Stage 8 (64-cell)
design: For each sample, 500-1000 embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the RNA II kit of Magerey Nagel and its quality was checked on a Bioanalyzer. All RNA samples had a RIN value higher than 9. Truseq Strand-specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).