title: GSM3179468: F1(G4) wt ESCs day 4 rep3; Mus musculus; RNA-Seq
library_construction_protocol: To isolate RNA either from heart tissue or cultivated cardiomyocytes the cells were lysed in 900 µl Qiazol (Qiagen, #79306). To remove the DNA 100 µl gDNA Eliminator solution was added and 180 µl Chloroform (AppliChem, #A3633) to separate the phases. The extraction mixture was centrifuge at full speed, 4°C for 15min. The aqueous phase was mixed with the same amount of 70 % Ethanol and transferred to a micro or mini columns depending of the amount of tissue and cells. The following steps were done according to the manufactural protocol. Libraries for RNA-seq were constructed according to the ScriptSeq v2 Complete (Mouse/Human/Rat) Low Input Kit (Illumina/Epicenter)