title: Whole genome sequencing with PacBio RS II (P6/C4) of Cucurbita argyrosperma subs. argyrosperma
design: Libraries were prepared and size-selected for a library size range of 8-30Kbp using the BluePippin system (Sage Science). Sequencing was done on 8 SMRT cells, using MagBeads to prioritize longer DNA fragments. Subreads were obtained after filtering 50bp minimum length and minimum quality score of 75.