title: GSM1428533: Zong 3; Zea mays; ncRNA-Seq
library_construction_protocol: Total RNA from the all samples was extracted with TRIzol reagent (Invitrogen, 15596-026) following the manufacturer’s protocol. Small RNA libraies were constructed by TruSeq Small RNA Sample Prep Kit (Illumina) following the manufacturer’s instructions. Briefly, 1 µg total RNA or purified small RNA was ligated with 3’ and 5’ adaptors and used as a template for RT-PCR. After PCR amplification, 6 µL of each sample were pooled and separated on a 6% polyacrylamide Gel. Gel slices corresponding to ~18-26 nt small RNAs were recovered.