title: Sunflower-control
design: Cotyledons from the sunflower were surface sterilized and inoculated in ½ MS media for germination and enlargement. From these seedlings, explants like root, hypocotyl, cotyledonary leaf and primary leaf were taken, cut into 0.5 cm size and inoculated onto MS agar medium supplemented with different concentrations and combinations of growth regulators for callus induction. The inoculated cultures were maintained at 27 ± 1 ºC under a 16/8 hr light/dark photoperiod with light intensity of 30 µmol m-2 s-1. Good regenerable callus was seen on 2-iP (2 mg/l) + TDZ (0.1 mg/l) + IAA (0.5 mg/l) media from the cotyledonary explants. For RNA- seq experiment, callus was collected at 0 days for the control sample and the RNA was extracted followed by sequencing.