title: Metatranscriptome of honey bee (Apis mellifera) digestive tracts
design: Total RNA was extracted from three samples (complete digestive tracts from three individuals) using a TRI reagent-based method coupled to lysis by grinding with a mortar and pestle under liquid nitrogen. To deplete the eukaryotic RNAs in each sample, we utilized the Ambion MicrobEnrichM-bM-^DM-" (Life Technologies, NY) kit on the total volume of each RNA sample, and followed the prescribed protocol The Illumina TruSeq RNA Sample Preparation protocol was used without modification to prepare for multiplexed sequencing. Following purification with 1X Agencourt Ampure XP beads, double-stranded cDNA samples were eluted in EB buffer and assayed on a D1K High Sensitivity Tape Station (Agilent) before cluster formation and sequencing on two lanes of a single flow cell run (100 x 100 bp paired end) on the Illumina Genome Analyzer II IIx system.