title: Deep Sequencing of Zaire Ebolavirus Strain Mayinga Vero Cells 06hpi
design:  Deep RNA sequencing of Vero infected cells with Zaire Ebolavirus                 Mayinga Strain at multiplicity of infection of 3. RNA was extracted with TriZol,                 poly-A RNA purified with Invitrogen Dynal oligo dT magnetic beads and then RNA-Seq                 libraries created using NEB Next mRNA-Seq kit using 18 cycles of PCR. Samples                 sequenced on Illumina HiSeq 2500, using 100nt single read                 settings. 