title: GSM3399112: TLR4-KO ethanol rep1; Mus musculus; miRNA-Seq
library_construction_protocol: Cortex were removed and frozen on nitrogen liquid. RNA was isolated using miRNeasy mini Kit (Appendix A, Qiagen, Hilden, Germany). Libraries were prepared with Truseq library prep Small RNA Sample Preparation kit (Illumina, San Diego, USA) 100ng of smallRNA fraction was used for the construction of sequencing libraries. The small RNA libraries were prepared for sequencing using standard Illumina protocols