title: GSM3573366: PAS-CLASH: control-1; Homo sapiens; OTHER
library_construction_protocol: Crude nuclei were prepared  using a nuclear/cytoplasm fractionation kit (BioVision).Total Nuclear cross-linked RNAs were prepared using Trizol reagent, and were further fragmented to 60-200 nt sizes using RNA fragmentation buffer (Thermofisher).RNA-RNA ligation was carried out on oligo d(T) beads using T4 RNA ligase (NEB). Forcross-link reversal, RNA was resuspended in TS buffer and was irradiated for 20 min at 254 nm. PAS-CLASH libraries were prepared using the photoreversed RNAs and QuantSeq Rev 3' mRNA sequencing library prep kit (Lexogen,Cat. 016.24), and sequenced with Hiseq.