title: GSM3573341: YRT230; Mus musculus; RNA-Seq
library_construction_protocol: Samples of 500ng of total RNA (but 100ng for YRT-236, and 200-300ng for YRT-224, -230 and -235)  were used as provided by the user. Average sample RNA Integrity Number was 8.5 (range 7.9-10) when assayed on an Agilent 2100 Bioanalyzer. PolyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in "NEBNext Ultra II Directional RNA Library Prep Kit for Illumina" (NEB #E7760) [this kit incorporates dUTP during 2nd strand cDNA synthesis, which implies that only the cDNA strand generated during 1st strand synthesis is eventually sequenced]. Adapter-ligated library was completed by PCR with Illumina PE primers. The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument (see below) by following manufacturer's protocols.