title: Illumina sequencing of soil samples: strawberry fields
design: Forty soil samples were collected from different points of the five strawberry production areas. The total soil genomic DNA was extracted using a direct bead-beating extraction method. Then, the  ITS1 region of the fungal rRNA gene was amplified using the ITS1-F and ITS2 primer pair containing the appropriate Illumina adapters for both the forward and reverse primers, 2 basepair “linker” sequences and a 12-bp error-correcting Golay barcode at 3′ end of reverse adapter unique to each sample. The PCR products were purified and pooled to acquire five amplicon libraries corresponding to the five different plantation areas and an equimolar mixture of all five amplicon libraries was sequenced using an Illumina MiSeq sequencer.