title: GSM3765302: E10.5_Pitx2_null_1; Mus musculus; RNA-Seq
library_construction_protocol: Hearts were digested in trypsin to generate a single cell suspension. Following digestion, heart tissue was washed with DMEM with 10% FBS to inactivate residual trypsin activity. Next, cells were washed twice with 1X PBS containing 0.04 % BSA before being counted. Finally, cells were loaded onto the 10X Genomics Chromium instrument. The scRNA-seq libraries were generated using the 10X Chromium Single Cell 3' v2 reagent kit according to the manufacturer's instructions and were sequenced on an Illumina Nextseq500.