title: GSM1145591: Sample 4 (d12); Apis mellifera; RNA-Seq
library_construction_protocol: Total RNA was extracted from the HGs of the samples (each pooled from 30 honey bees) using TRIzol reagent (Invitrogen, USA). A Qubit fluorometer (Invitrogen, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA) were used to determine the quality and quantity of the RNA. The mRNA was enriched using oligo (dT) magnetic beads, then fragmented into short fragments (approximately 200-700 nt) using fragmentation buffer (Invitrogen, USA). Double-stranded cDNA product was synthetized and purified . sequencing adapters were ligated to the fragments, and the fragments were purified by agarose gel electrophoresis (AGE) and enriched by PCR amplification. The library products were sequenced using the Illumina HiSeqTM 2000 system.