title: GSM1145623: Mnase_WT11_ZT06; Mus musculus; MNase-Seq
library_construction_protocol: Adult mouse liver were collected at 6 different time of the day (mouse held under LD12:12, time points every 4hrs starting at ZT2) Mouse liver were crosslinked with 1% formaldehyde for 10min, and nuclei were purified using standard sucrose cushion protocol. Nuclei were resuspended, flash frozen in liquid nitrogen and stored at -80C. Nuclei were thawed on ice, pelleted down and used for micrococcal nuclease diggestion. Mnase digestion was performed for 15min using 30,000 gel unit per reaction DNA was extracted (size ~150nt)  and about 50ng of purified DNA was used for generating Illumina libraries Illumina libraries were generated using standard protocol: end repair, add A, adapter ligation, amplification, gel purification.