title: GSM1553119: TAG RAS/DNP53 HAsiRNA rep2 [140521_SND393_B_L003_HBY-18_R1]; Mus musculus; RNA-Seq
library_construction_protocol: Animals were sacrificed 30 minutes after the last siRNA injection, tumors were dissected and RNA was extracted using Trizol reagent. The libraries were prepared with the TruSeq Stranded mRNA sample prep kit from illumina according to the manufacture's instructions. After oligo d(T) purification, mRNA was fragmented by zinc. cDNA was synthesized using random primer. Adapters are then ligated and PCR cycles were performed to enrich the library. The libraries were sequenced on a HiSeq 2500 instrument (from illumina) in a single read 1x100bp. The v3 chemistry was used according to manufacture's instructions.