title: Draft genome of the emerging multidrug resistant pathogenic fungus Candida auris isolate UT6684
design: Library preparation was performed at Genotypic Technology’s Genomics facility following NexteraMate Pair Gel Plus protocol outlined in “Illumina Nextera Mate Pair library preparation guide (Cat# FC-132-9001DOC, Part#15035209 Rev D.)”. ~4 gof Qubit quantifiedDNAwas taken for Tagmentation. The tagmented sample was cleaned up using AMPure beads and subjected to strand displacement. 3-5kb range of the strand displaced sample was size selected on 0.6% Agarose. Size Selectedsample was taken for circularizationovernight,followed by linear DNA digestion with DNA Exonuclease. The circularized DNA molecules were sheared using Covaris to obtain fragments in the size range of 300 to 1000bp. Sheared DNA was subjected to bead binding with M280 Streptavidin beads to isolate biotinylated molecules. End repair, A-Tailing, Adapter ligations were performed on the bead-DNA complex. Adaptor ligated sample was amplified for 15 cycles of PCR followed by AMPure XP bead clean up.