title: Metagenomic sequencing of marine sediment sample
design: 16S V3-V4 Metagenome libraries were prepared by Indexing PCR adding Illumina sequencing barcoded adaptors [5 - AATGATACGGCGACCACCGAGATCTACAC [i5] TCGTCGGCAGCGTC, 5 - CAAGCAGAAGACGGCATACGAGAT [i7] GTCTCGTGGGCTCGG] and analyzed on 1.2% agarose gel. Further the libraries were normalized and pooled for sequencing.