title: GSM1551258: LOS #4 Liver; Bos indicus x Bos taurus; RNA-Seq
library_construction_protocol: At tissue collection, liver, skeletal muscle, brain, and kidney were well diced, mixed, and snap frozen in liquid nitrogen and stored at -80°C. Total RNA from fetal tissues was isolated using Trizol Reagent (Invitrogen) according to the manufacturer's instructions. RNA quality was determined by both spectrometry and agarose gel electrophoresis. For each sample, 5μg RNA was submitted to the DNA core at University of Missouri, Columbia for RNAseq library preparation using Illumina TruSeq RNA sample preparation kit. Briefly, poly-A containing RNA was purified using magnetic beads with oligo-dT attached. The purified RNA was fragmented and used as a template for cDNA synthesis using random primers. In the following end-repair procedure, an "A" was added to the 3' end of the cDNA. The cDNA fragments with A-tailing were then ligated with T-tailing adaptors. PCR amplifications of cDNA fragments were followed to generate the final RNAseq library.