title: GSM3573259: SCNT_CROC+K_2; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq experiments with total RNA samples (50~100 embryos) extracted from 2-3 biological replicates were performed. Total RNA was extracted from SCNT_FOC+K, SCNT-CROC+K and SCNT_CROC+K+M embryos using RNeasy Plus Mini Kit (74134, Qiagen) and its amount and quality of the total RNA were evaluated using Bioanalyzer (Agilent). RNA samples with >7.0 RNA Integrity Number (RIN) value were used for RNA-Seq library preparation using combined SMARTer Ultra Low Input RNA cDNA preparation kit (TAKARA) with ScriptSeq v2 kit (Illumina) according to manufacturer's instruction. Libraries were prepared for sequencing using standard Illumina protocols