title: RNA -Seq of Streptomyces albulus: Varying salt concentrations
design: Total RNA was rRNA depleted using RiboZero. mRNA was converted into template molecules using the TruSeq Stranded total RNA Sample preparation kit and indexed using the Nextera indexing kit (Illumina). Paired-end (2 x 250 bp) sequencing was performed using the MiSeq reagent kit v2. Demultiplexing and quality trimming, with adapter removal, were performed with Casava 1.7 (Illumina).