title: GSM3574392: WT_PDL_rep2; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq,cells were treated with TRIzol and followed with phase seperation,  RNA precipitation and RNA wash. Finally, RNA were dissolved in DEPC water.For RIP-seq,The RNA in input lysis and  precipitated beads were extracted by TRIzol. For RNA-seq,total RNA was subjected to two rounds of purification using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds cDNA was ligated to adaptors and sequenced with illumina Hiseq2000 platform.For RIP-seq,Input RNA were treated as the total RNA in RNA-seq,the IP RNA RNA from RIP experiment was directly used for the synthesis of ds-cDNA.