title: mESC_holo_1ng_2
design: Total RNA was extracted from approximately one million mESCs using                 an RNeasy Plus Mini kit (QIAGEN), 1ng diluted total RNA was mixed with with 100 ng                 carrier RNA mixture. The sequencing library was constructed following the                 manufacturer's protocol using NEBNext kits (E7490 & E7530). After adaptor                 ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not                 I digestion. After Not I digestion, the library DNA was purified for PCR                 amplification.