title: GSM4365596: H3.3-FLAG_24h_4OHT; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin was isolated from formaldehyde cross-linked cells, sonicated and histone-DNA complexes were immunoprecipiated with antibodies. Libraries were constructed using the KAPA LTP library preparation kit (KAPA Biosystems KR0453-v6.17) according to manufacturer's protocol. After adaptor ligation, 14 PCR cycles were used for library amplification and ~300 bp fragments were size selected using Agencourt AMPure XP beads (Beckman Coulter).  The libraries were sequenced as 65 base single reads on a HiSeq2500 (Illumina).