title: RNAseq of Ogataea polymorpha NCYC495 : MATa/alpha diploid in NaKG media
design: To examine the transcriptional response induced by nitrogen depletion, we used mRNAseq to compare the transcriptomes of O. polymorpha cells 2 h after transfer from a YPD pre-induction culture into NaKG, to parallel cultures transferred into fresh YPD. Furthermore, because we expect that switching occurs only in haploid cells, we conducted this experiment in parallel on haploid (MATa and MAT_ isogenic strains) and diploid (MATa/_) cells.