title: GSM3574700: wild type 53 °C [C_37-53C_III]; Bacillus subtilis; RNA-Seq
library_construction_protocol: Total RNA was isolated from flash-frozen cells using phenol/TRIzol extraction as described in Lamy et al. (2004) Molecular Microbiology, 54: 1250-1268 and treated with DAase using the TURBO DNA-free kit (Ambion). RNA integrity was checked on a Bioanalyzer 2100 rRNA was depleted using the MICROBExpress Kit (Invitrogen). mRNA was supplemented with ERCC ExFold RNA Spike-In Mixes, treated with 5'RNA-polyphosphatase, sonicated and treated with PNK. The library was generated using the NEB Next Small RNA Kit as described by Manufacturer. In addition, one replicate without ERCC was not treated with polypphosphatase