title: GSM3181654: K56 rep2; Klebsiella pneumoniae; RNA-Seq
library_construction_protocol: RNA was isolated by growing the isolates to the logarithmic phase in high osmolarity LB broth at 37°C and extracting the RNA using RNeasy® mini kit (Qiagen GmbH, Hilden, Germany).  On-column DNA digestion was carried out using RNase-free DNase Kit (Invitrogen, Carlsbad, CA, USA). RNA concentration was measured using a NanodropTM spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Library preparation was carried out using TruSeq Stranded total RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Illumina NextSeq 500 sequencer (Illumina, San Diego, CA, USA) was used to carry out RNA sequencing.