title: GSM1151031: E14(wt)_RNA; Mus musculus; RNA-Seq
library_construction_protocol: RNA was prepared using Trizol, according to the manufacturer's instructions. The RNA was examined for quality by Bioanalyzer (Agilent). RNA-seq libraries were then prepared as described in Wamstad et al., 2012. Briefly, mRNA was purified and fragmented, first strand cDNA was synthesized, and the second strand was synthesized. The SPRI-TE Nucleic Acid Extractor by Beckman Coulter was then used to automate library preparation. Each sample was amplified for 18 cycles. The samples were then examined for proper size and structure by Bioanalyzer (Agilent) and qPCR.