title: GSM4086521: TKO1; Mus musculus; RNA-Seq
library_construction_protocol: One tumor was dissected from the lung of each mouse, chopped and digested in 1ml of PBS containing 2mg/ml of Collagenase/Dispase (Roche) in a 37°C shaker for 45 minutes. Tumors were then cooled on ice, treated with 2.5μg/ml DNAse (Sigma) for 5 minutes and passed through a 40μm filter. Red blood cells were lysed and the sample was single cell-sorted by FACS. Single cell capture and library generation was performed using the Chromium Single Cell Controller from 10x Genomics.