title: GBS of Lagenaria siceraria
design: Fresh leaves were used for DNA isolation using the DNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA). Genotyping-by-sequencing was used for simultaneous discovery and genotyping of SNP markers for hard-shelled and soft-shelled types of bottle gourd according to the protocol described by Elshire et al. (2011). Prepared DNA libraries were sequenced in an Illumina HiSeq platform (Illumina, San Diego, CA) at the Institute for Genomic Diversity (Cornell University, Ithaca, New York, USA).