title: RNA-Seq of SKOV-3 NM-CSCs_1
design: Oligo (dT) magnetic beads are used to select mRNA with polyA tail. Then obtain the target RNA after purification. 2) Fragment the target RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random primer. 3) Endrepair the dscDNA with phosphate at 5' end and stickiness ' A' at 3' end, then ligate and adaptor with stickiness ' T' at 3' end to the dscDNA. 4) Two specific primers are used to amplify the ligation product. 5) Denature the PCR product by heat and the singl e strand DNA is cyclized by splint oligo and DNA ligase. 6) Perform sequencing on prepared library.