title: Knock-in of PIK3CA-H1047R into MCF-10A
design: DNA for each sample was sheared on an S2 Covaris to a size range of 250-400bp. 1 ug of sheared DNA for each sample was then end repaired, A-tailed with Taq polymerase, kinased and ligated to standard Illumina TruSeq barcoded adapters following Illumina recommended protocols.  The library was them PCR amplified for 6 cycles. The amplified libraries then hybridized to Agilent Human All Exon Sureselect Target Enrichment baits following manufacturer’s recommended protocols. After hybridization, the selected libraries were amplified for 12 more cycles, sized selected on a 2% agarose gel to recover library products with insert sizes in the 250-350 base size range. Size selected libraries were loaded onto an Illumina sequencer for paired-end 2 x 100 base sequencing. Raw sequencing data was processed into fastq files using CASAVA 1.8 (Illumina).