title: GSM2856092: WTTEC_P1_10; Mus musculus; RNA-Seq
library_construction_protocol: Thymic stromal cell isolation was done with slight modifications as described earlier (Rode et al., 2015). In contrast to the published method, minced thymi were directly subjected to digestion without discarding any fractions, and lymphoid cells (anti-CD45 beads, Miltenyi Biotec) and erythrocytes (anti-Ter119 beads, Miltenyi Biotec) were manually depleted using MACS technology. As described in CEL-Seq2 protocol (Hashimshony et al. 2016) Adapted from TruSeq Small RNA Library Preparation Protocol