title: Fungi in roots and rhizosphere of Rubiaceae
design: DNA was extracted from root tissue of trees in the Rubiaceae using the DNeasy Plant Mini Kit (QIAGEN Inc., Hilden Germany) and  DNA was extracted from rhizosphere soil associated with the collected roots using the MoBio PowerSoil Kit (QIAGEN Inc., Hilden Germany), following the manufacturers protocols except that purified DNA extracts were stored in TE buffer. The internal transcribed spacer (ITS2) region of DNA was amplified using the ITS4 forward primer (TCCTCCGCTTATTGATATGC; White et al. 1990)  and the 5.8SR reverse primer (TCGATGAAGAACGCAGCG; https://sites.duke.edu/vilgalyslab/rdna_primers_for_fungi/) and were sequenced using Illumina high-throughput sequencing with a unique index adapter for each sample.