title: GSM4292629: AB2217; Mus musculus; RNA-Seq
library_construction_protocol: Hepatic CD4+ or CD8+ T-cells  were isolated by liver perfusion, mechanical + enzymatic digestion and percoll gradient enrichment and subsequently single-cell sorted (sorted gate: L/D-,CD11b-,CD11c-,CD19-,CD45+,CD4+ or CD8+ cells) and frozen on dry-ice .​ Samples were flow sorted either using AriaII, AriaIII or Aria-Fusion (BD Biosciences, BD Diva Software) cell sorter. 3' end mRNA libraries were prepared for sequencing using a modified MARS-seq protocol (Jaitin et al, Science 2014)