title: ChIP-seq profiling of human 501mel melanoma cells
design: The human melanoma cell line 501mel was treated with DMSO (vehicle control) or AZD6244 for 72 hours, then 10 million cellswere crosslinked with 1% formaldehyde for chromatin immunoprecipitation (ChIP). Samples from no antibody control (input) and ChIP using specific antibody were reverse-crosslinked and DNA was purified using the phenol-chloroform extraction method. Aliquots of DNA purified from input and ChIP  (from two independent experiments) samples were used for library construction and high-throughput sequencing as single-end 50-mer reads.