title: RNA-seq of CD8+ t cells mus mu8sculus
design: The libraries were prepared following the Smart-seq2 protocol71. Five-hundred pg of cDNA from each sample were tagmented and amplified using Illumina Nextera XT kit. The resulting libraries were pooled, double-sided size selected (0.5x followed by 0.8x ratio using Beckman Ampure XP beads) and quantified using an Agilent 4200 TapeStation System. The pool of libraries was sequenced in an Illumina NovaSeq6000 sequencer (single-end 100 bp) with a depth of around 20 Mio reads per sample as a service by the Functional Genomics Center Zurich (FGCZ).