title: Activation of Cardiac Enhancers and Inhibition of the Inflammatory Response Promotes Cardiac Reprogramming of Adult Fibroblasts
design: TTFs were transduced with indicated retroviruses. Two days after retroviral transduction, TTFs were crosslinked with 1% formaldehyde in PBS for 30 min and neutralized by the addition of glycine to a final concentration of 0.125 M for 5 min. TTFs were harvested and washed with cold PBS, then perform ChIP using indicating antibodies and ChIP-IT® Express Chromatin Immunoprecipitation Kits (Active Motif) according to according vender’s protocol.