title: GSM3443343: MeDIP.T2; Mus musculus; MeDIP-Seq
library_construction_protocol: For MeDIP-seq, genomic DNA (gDNA) was extracted from cells using a DNeasy Blood and Tissue kit (Qiagen, Chatsworth, CA, USA) according to the manufacturer's instructions. DNA sonication, Illumina's paired-end library preparation and methylated DNA immunoprecipitation were performed as previously described (Feber et al., 2011). Briefly, five micrograms of gDNA were randomly sheared to a median fragment size of 200 bp using a Covaris S220. Sonicated DNAs were subjected to library preparation according to Illumina Paired End libraries preparation protocols. Then methylated DNA immunoprecipitation was performed using the Methylated-DNA IP kit (Zymo Research; Orange, CA, USA). MeDIP libraries were prepared for sequencing using standard Illumina protocols.