title: RNA-Seq of Saccharomyces cerevisiae BY4147 10min at 42C
design: The RNA was isolated from logarithmic growing, heat-stressed yeast cells (42 C, 10 min)  according to the TruSeq RiboProfile Kit (yeast; Illumina).  Subsequently, he rRNA was depleted from the total RNA sample according to the Ribo-Zero Magnetic Gold Kit (Illumina) procedure. Fragmentation of the total RNA samples, end repair, 3 adapter ligation, reverse transcription cDNA circularization and PCR were performed as described in the TruSeq RiboProfile Kit. The libraries were sequenced on a HiSeq1500 sequencer (Illumina).