title: GSM3866424: Mouse_amygdala2; Mus musculus; RNA-Seq
library_construction_protocol: Bilateral amygdalar Vibratome 1000-sliced biopsy tissue was homogenized, bathed in RNAase-free phosphate-buffered saline, and centrifuged. The precipitate was then extracted and snap frozen in liquid nitrogen. The tissue was placed in Trizol (ThermoFisher). Ribonucleic acid was extracted using the phenol-chloroform phase separation and prepared for subsequent RNA Library preparation. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on a single Illumina NextSeq500 run with single-end 75bp reads