title: GSM3184563: MDA-MB-231_MEL-18 WT_Input; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates from cells cross-linked with 1% formaldehype were sonicated and incubated with anti-FLAG antibody to isolate the FLAG-tagged protein-DNA complexes. The purified genomic DNA were quantified using Quant-IT PicoGreen (Invitrogen) and qualified using the Agilent High Sensitivity DNA Kit and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). The sequencing libraries were prepared according to the manufacturer's instructions of the TruSeq ChIP Sample Preparation Kit (Illumina). The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide (KAPA Library Quantification kits for Illumina Sequencing platforms) and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies, Waldbronn, Germany). Indexed libraries were then sequenced using the HiSeq2500 platform (Illumina).