title: Illumina HiSeq 2000 sequencing; Analysis of post-transcriptional RNA modifications in Caenorhabditis elegans under stress
design: Analysis of post-transcriptional RNA modifications in Caenorhabditis elegans under stress
library_construction_protocol: Animals were harvested and washed 3X in M9 buffer. Syncronised population of L1 animals were grown until young adult stage. For heat stress, animals were heat-shocked at 37℃ for 4hrs with food.  For starvation experiments, young adult stage animals were  plated on either food plates for control or on empty plates for starvation at 20℃ for 4hrs. Settled animals were mixed with Trisure reagent, bead beaten using zirconia beads, and the RNA is extracted using chloroform. For total RNA sequencing, Illumina Ribozero kit is used to remove ribosomal RNA from 1ug of total RNA prior to library preparation. RNA sequencing libraries are prepared using NEB Next Ultra library preparation kit.