title: GSM3868675: repA ESRRB 8h; Mus musculus; ChIP-Seq
library_construction_protocol: Cells we fixed in 1% formaldehyde at RT for 10 minutes and subsequently 2mM disuccinimidyl glutarate , nuclei extracted and chromatin sonicated using a bioruptor (10 cycles, 30s on 30s off). Cleared chromatin was incubated with anti-ESRRB (R&D, PP-H6705-00), or anti SOX2 antibody (R&D, AF2018). Input DNA serves as negative control for antibody enrichment. Libraries were prepared using the NEB Next Ultra 2 kit.