title: GSM3182468: HH28_DHL_WT_H3K27ac; Gallus gallus; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitated experiment was performed as previously described (Beccari et al. 2016). Micro-dissected limb tissues from mouse and chick embryos were cross-linked with 1% formaldehyde/PBS for 15 min at room temperature. Chromatin was sheared and used for each immunoprecipitation with anti-H3K27ac (ab4729, Abcam), anti-H3K27me3 (07-449, Merck Millipore), anti-H3K4me1 (ab8895, Abcam) and anti-CTCF (61311, Active Motif), respectively. Libraries were prepared with at least 5 ng of purified DNA following Illumina TruSeq ChIP library preparation guide. Sequencing was performed with 100-bp single-end.