title: GSM3443282: 274L_S7; Homo sapiens; RNA-Seq
library_construction_protocol: Buds were detached and separated from the spheroids at stage 5 of the differentiation protocol by mechanical agitation and filtering. RNA-sequencing of buds versus bulks was performed on samples from 5 separate differentiation flasks, whereby bulks could be collected from all and buds from 3 of the differentiation flasks. Polyadenylated RNA was selected from total RNA using the PrepX PolyA mRNA Isolation Kit (Takara Bio USA) and sequencing libraries were prepared using the PrepX RNA-Seq for Illumina Library Kit (Takara Bio USA). After additional enrichment, indexing and cleanup steps, libraries were sequenced on an Illumina NextSeq 500.