title: RNA-Seq of Radula marginata
design: RNA collected from each of six samples was pooled and 2.5  g of RNA was used for the preparation of the cDNA library. Library was prepared according to the instruction of Illumina  (Illumina, San Diego, USA). The further step for the preparation of the library like adapter insertion, interruption of the fragment, size selection as well as PCR amplification was carried out at IGA Technology, Italy. Illumina Next generation Sequencing HiSeq-2000 was used as a platform for 250 bp paired end sequencing at the depth of 60 million reads.