title: GSM3398680: KO_Igg; Mus musculus; ChIP-Seq
library_construction_protocol: After 4-day puromycin treatment, GFP- and Cre-BMSCs were fixed by 1% formaldehyde for 10 min and terminated by glycine for 5 min (final concentration = 0.125 M). After 2 times washing by pre-cooling PBST (protease inhibitor containing), cells were scraped and resuspended in SDS-lysis buffer respectively (50 mM Tris-HCl Ph 7.5, 10 mM EDTA, 1% SDS and protease inhibitor), and sonicated. Cells were centrifuged to obtain cell extracts, which then were added to pre-cleaning protein G agarose and rotated for 1 hr at 4 ℃. Extracts were centrifuged and supernatants were harvested to new tube. ChIP assay was performed using H3K36me3 antibody(abcam,ab9050). Normal IgG(Millipore,12-371) was used as negative control. Libraries were prepared according to Illumina's instructions