title: GSM1434787: MCIDAS_dnE2F4_9h_RNAseq_rep_2; Xenopus laevis; RNA-Seq
library_construction_protocol: mRNAs were obtained with proteinase K digestion, phenol chloroform extraction and precipitated with ethanol, treated with Dnase, extracted with phenol chloforom, and precipitated with ethanol. For ChIPseq embryos were first fixed in 1% formaldehyde for 30 minutes and lysed in RIPA buffer prior to sonication. See our manuscript (PMID: 24934224) for a more detailed protocol. RNAseq libraries were constructed with Illumina Truseq v2 non-stranded mRNA kits. Following IP, ChIPseq libraries were constructed with end repair mix (NEB), a-tailed (NEB), Truseq v2 adapters ligated (Illumina, NEB) and amplified with Phusion polymerase (NEB).