title: WGS of mus musculus
design: Genomic DNA was sheared to 200-300bp fragments using Covaris S2 and used to construct a sequencing library using the Illumina Truseq PCR free library construction tool according to the manufacturer's recommended protocol. The sequencing library prepared by random fragmentation of the DNA, followed by 5' and 3' adapter ligation. Adapter-ligated fragments are then PCR-amplified and gel-purified. For cluster generation, the library was loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. Each fragment is then amplified into distinct, clonal clusters through bridge amplification.