title: RNA-Seq of sheepgrass seeds:Y28-2
design: mRNA was enriched by oligo(dT) and fragmented into short sequence.The first strand of cDNA was synthesized with random6 primers. The second strand of cDNA was synthesized with DNA polymerase I and RNase H.The double strand cDNA was purified with AMPure XP beads. Then the adaptors were ligated into the ends of cDNA. Next, the PCR was performed using this cDNA as template. The target fragment of PCR was purified with AMPure XP beads and used for sequencing.