title: GSM1434988: Wild-type cortex sample 3; Mus musculus; RNA-Seq
library_construction_protocol: Flash frozen tissues were homogenized in 1ml Trizol (Life Technologies) using 5mm stainless steel beads for 2 minutes at 25 Hz with a TissueLyserII (Qiagen). Total RNA was isolated using Trizol according to the manufacturer’s protocol. Ambion PureLink RNA Mini Kit (Life Technologies) was utilized for RNA Clean-Up and DNase. In short, 15ug total RNA mixed with lysis buffer:ethanol (1:3) was applied to the column and rinsed with Wash Buffer II (70% ethanol). DNA was digested on column for 15 minutes with PureLink DNase (Life Technologies) and RNA was rinsed twice with Wash Buffer II (70% ethanol). Sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA with Ribo Zero kit (Illumina). The libraries were multiplexed three to a lane, grouped in biological triplicates. 100 base pair, paired-end sequencing was performed using the Illumina HiSeq platform.