title: Structure-seq of C briggsae_DMS_replicate 2
design: The L4 stage worms were covered in the reaction buffer with 40 mM HEPES (pH 7.5), 100mM KCl, and 0.5 mM MgCl2. For (+) DMS treatment, DMS was applied to a final concentration of 0.75%  with gentle mixing. For (-) DMS treatment, the reaction buffer was used as control. Treatment was performed for 3 min at 20C with gentle mixing. Dithiothreitol was used to quench DMS at a final concentration of 0.5 M with gentle mixing. The RNA was subject to the Structure-seq library construction.