title: GSM3439737: Single Cell RNA-seq, BM-hMSC-TERT4 osteoblast diff 7d; Homo sapiens; RNA-Seq
library_construction_protocol: Single Cell RNA-seq: Cells have been trypsinized and spun down at 200 g and washed twice in culture media. Cells were separated using a 40 µm mesh, washed 3 times in PBS with 0.04% non-acetylated BSA, and cell numbers were adjusted to 0.7 million cells per ml followed by a final transfer through a 40 µm mesh. Single Cell RNA-seq: Libraries were prepared according to manufactures instructions (10x Genomics) and sequencing reads were processed using the Cell Ranger pipeline