title: Genome sequence of Lactobacillus delbrueckii subsp. bulgaricus KW14_3
design: For Illumina pair-end sequencing of each strain, at least 1g genomic DNA was used for sequencing library construction. Paired-end libraries with insert sizes of ~300bp were prepared following Illuminas standard genomic DNA library preparation procedure. Purified genomic DNA is sheared into smaller fragments with a desired size by Covaris, and blunt ends are generated by using T4 DNA polymerase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag could be introduced into the adapter at the PCR stage as appropriate and we did a library quality test. At last, the qualified Illumina pair-end library would be used for Illumina Miseq sequencing