title: GSM3399097: KPC-Par1-KO8_Thrombin_Rep4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was islated using the miRNAeasy isolation kit (Qiagen, 217004) per the manufacterurs instructions. Libraries were constructed by the Purdue Genomics Facility using the TrueSeq Stranded Total RNA with Ribo-Zero Globin kit (Illimina, San Diego, CA). Paired-end, 2x100 bp reads were sequenced using the HiSeq2500 on high-throughput mode.  Before library preparation the dscDNA quality was checked using an Agilent Bioanalyzer with the High Sensitivity DNA Chip.