title: RNA-seq profiling of human 501mel melanoma cells
design: The human melanoma cell line 501mel was treated with DMSO (vehicle control) or AZD6244 for 72 hours, then the cells were harvested with TRIzol reagent for RNA extraction. RNA was extracted using the Direct-zol RNA Miniprep Kit and 200 ng RNA was used to generate 125 bp paired-end poly-A enriched RNA-seq libraries.