title: Transcriptome sequencing analysis of Ipomoea batatas
design: Sweet potato [I. Batatas (L.) Lam. cv. Xushu 18] was grown under normal conditions in Chengdu, Sichuan Province of China. Samples of leaves were collected after planting.Total RNAs were extracted from samples by using the Trizol Reagent (Invitrogen, USA), and treated with DNase I (Fermentas, USA) according to the manufacturer’s instructions. RNA concentrations were measured with Qubit fluorometer (Invitrogen, USA).the paired-end cDNA library was prepared with an insert size of 200 bp and submitted to Illumina Hiseq 2000 platform for sequencing at Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, China.