title: GSM2356701: 1MDP; Mus musculus; RNA-Seq
library_construction_protocol: GMPs and MDPs were isolated from the pooled bone marrow of 20 mice and cultured in vitro in media (RPMI with 10% FCS and 1% penicillin-streptomycin) supplemented with M-CSF (50 ng/ml; Peprotech). On days 2, 3 and 4 of culture, cells were harvested and FACS sorted for monocyte progenitors (Ly6G− CD11b− c-Kit+ Ly6C+ CD115+) and Ly6Chi monocytes (Ly6G− CD11b+ c-Kit− Ly6Chi CD115+ F4/80−). Whole cell lysates of cells isolated at the three timepoints were pooled for each cell type, and RNA was extracted from using an RNeasy mini kit (Qiagen). Each sample was subjected to library preparation with a KAPA kit (Kapa Biosystems) and multiplexed across 4 lanes to yield a mean of 32.9 million 75 bp single-end reads per sample (standard deviation 1.7 million reads).