title: GSM4012685: TASC_CT, total bone marrow, sample 2; Mus musculus; RNA-Seq
library_construction_protocol: Fresh bone marrow cells were isolated by crushing mouse bones in media supplemented with FCS and the supernatant was collected. Cells were stained with fluorescently labelled antibodies against lineage markers (CD4, CD8, CD11b, B220, Gr1, Ter119) and c-Kit. A DNA dye was used to exclude debris. Single DNA+ cells were sorted into PBS-2%FCS. TASC-seq, 10x genomics based Libraries were prepared according to DropSeq protocol (Macosko Cell 2015) or 10X Genomics single-cell RNAseq v2 (Chromium 3' Reagent Kits v2 User Guide Rev C "CG00052") for whole transcriptome or TASC-seq protocol for targeted transcriptome readouts.