title: GSM3330257: isPTB-6; Homo sapiens; RNA-Seq
library_construction_protocol: placental villous tissue was flash frozen within an hour of delivery. Frozen samples were placed in RNALater Ice and thawed for homogenization and total  RNA extraction using the RNAeasy Mini kit (Qiagen). 50-100 ug of total RNA was submitted for quality assessment and sequencing. Long RNA libraries were generated using a RiboZero Kit (Illumina) using standard protocols. RNA-sequencing on Illumina 2000 HiSeq  at the University of Cincinnati Genomics, Epigenomics and Sequencing Core