title: GSM3574769: miR-146 3'F BMDMs (resting); Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA from BMDMs was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. TruSeq-barcoded RNAseq libraries were be generated with the NEBNext Small RNA Library Prep Kit (New England  Biolabs) and size selected for insert sizes ~18-35bp. Each library were quantified with a Qubit 2.0 (dsDNA HS kit;  Thermo Fisher).