title: F120-1
design: Single colony of strain F120-1 was precultured overnight with 3 mL LB medium in a rotary shak-er (200 rpm) at 37 . Then 1 mL of preculture was inoculated into 100 mL M9Y medium and anaerobiclly cultivated at the same conditions for 12 h, with E.coli W3110 served as a control. Each sample has three replicates. Genomic DNA (>10 g) was isolated with DNEasy Spin column kit (QIAGEN GmbH, Germany) and quantified with a Qubit Fluo-rometer and agarose gel electrophoresis before library construction. Then genomic DNA was sheared into smaller fragments with a desired size by Covaris E210 firstly. Then overhangs generated from fragmentation were repaired with T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After that an A base was added to the 3' end of the blunt phosphorylated DNA fragments, adapters were then ligated to the ends of the DNA fragments. The desired fragments were gel-purified, selectively enriched by PCR amplification. The index tag was introduced into the adapter at the PCR stage as appropriately. Then the qualified libraries were used for sequencing.