title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of Setaria viridis culms from control and SvBAHD01 RNAi plants
design: RNA-seq of Setaria viridis culms from control and SvBAHD01 RNAi plants
library_construction_protocol: plants were maintained in growth chamber (Fitotron) under 16 h photoperiod of 400 μ mol m−2s−1 light intensity, 26 ± 2 °C and 65% relative humidity agrobacterium transformation exactly as described in Martins et al. (2015) Biotechnology Reports 6: 41-44 Total RNA of stem was obtained using NEBNext® RNA Library Prep Set Kit for Illumina® following manufacturer's instructions.  Ribo-ZeroTM Magnetic Kit + NEBNext® Ultra™ RNA Library Prep Kit for Illumina®