title: GSM3330879: MK1; Mus musculus; RNA-Seq
library_construction_protocol: E15.5 embryonic kidneys were dissected and dissociated in 1mg/ml liberase (Roche) on ice for 25 minutes. Samples were gently agitated by pipetting every 5 minutes. Samples were filtered, washed once with cold PBS and pelleted by centrifugation. Samples were resuspended at the appropriate concentration and stored on ice prior to the generation of single cell libraries using the 10X platform. Chromium Single Cell Library kit V2 Illumina HiSeq HT chemistry- 100bp paired end sequencing