title: GSM1148268: HP1a-/- ESC RNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: Cells harvested and flash frozen using liquid nitrogen.  RNA was then extracted using Sigma GenElute Mammalian Total RNA Extraction Kit RNA-seq libraries were prepared from mRNA as described in Morin et al from 10ug of DnaseI treated total RNA (Morin R, Bainbridge M, Fejes A, Hirst M, Krzywinski M, Pugh T, McDonald H, Varhol R, Jones S, Marra M: Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing. BioTechniques 2008, 45:81-94.)