title: RNA-Seq of D. mojavensis from Cd. Obregon:adult female exposed to cactus diet
design: Newly emerged adult females from cactus diet were washed twice with distilled water and placed into 1.5ml tubes, then rinsed twice with 1X PBS. All liquid was then removed, and flies were frozen (-70oC) until RNA extraction. Frozen samples were homogenized with Tri-Reagent using Teflon homogenizers and extracted using Direct-zolTM RNA MiniPrep extraction kit (Zymo Research) according to the manufacturer's protocol. Libraries were prepared with TruSeq RNA Sample Preparation Kit v2 (Illumina), selecting only polyA mRNAs and synthezing double stranded cDNAs to attach to the Illumina adapters. Library size and quality were measure by Bioanalyzer (Agilent Technologies) and sequenced in a 1 X 100 single-end read format on a HiSeqTM 2000 Sequencing System (Illumina).