title: PARE-seq of zea mays: seedling shoot raw extracts
design: Total RNAs were extracted from raw extracts of maize (Zea mays, inbred line B73) seedling shoots with TRI Reagent (Molecular Research Center, USA) and used for PARE (parallel analysis of RNA end) library construction according to the method describe by Zhai et al. [Zhai J, Arikit S, Simon SA, Kingham BF, Meyers BC. Rapid construction of parallel analysis of RNA end (PARE) libraries for Illumina sequencing. Methods. 2014;67:8490.].