title: GSM4086252: glp-4_pos-1_PolyP_Ox_sRNA_setD; Caenorhabditis elegans; OTHER
library_construction_protocol: Small RNAs (~16-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs and/or oxidized with sodium periodate to distinguish methylated small RNAs (as indicated in the molecule section), followed by ligation of preadenylated 3' adapters and non-adenylated 5' adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~134-146 bp) were size selected from 10% polyacrylamide gels Added to SAMPLES section. Added to SAMPLES section.