title: GSM2856208: PBS-1; Mus musculus; RNA-Seq
library_construction_protocol: Tרeated mice where euthanized, femur and tibia extracted, crashed in sample medium (PBSx1, EDTA 2mM, serum 2%) and loaded on Histopaq1083. this mononuclear enriched population was stained and sorted for LSK150+mC+ cell, 1000 cells from each sample, to SMARTseq buffer. library construction and sequencing was performed by the INCPM facility (Rehovot) SMARTSeq Ultra Low Input RNA V4 kit from Clontech (polyA based capture), with 14 cycles of cDNA amplification. The amplified cDNA was fragmented using the Covaris machine to around 450bp. Illumina libraries were constructed using the End repair, A base addition and adapter ligation (TruSeq like adapters:GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGCCTTATCTCGTATGCCGTCTTCTG) and amplified using 13 PCR cycles