title: Km, mRNA enriched from Ka_RNA_illumina - Illumina HighSeq2000 metatranscriptome
design: Phenol/chloroform extraction, Ribodepleted/mRNA enriched using the MicrobExpress (Ambion), Ribominus Bacteria (Invitrogen) and Ribominus Eukarya (Invitrogen). mRNA linearly amplified using the Message amp II bacteria kit, ampRNA was reversely transcribed with random hexamer primed cDNA synthesis, sequenced with Illumina HighSeq2000.