title: Amplicon sequencing of V. cholerae viuB  August-Lagoon water 5um size fraction
design: Libraries were constructed by PCR amplification of the viuB-gene using Vibrio cholerae specific primers followed by additional PCR cycles with dual-indexed primers based on Kozich et al 2013 AEM(79) 17 5112-5120 to create products for subsequent sequencing with Illumina Miseq V3 600 cycle kit