title: GSM3032052: miRNA N.brasiliensis exosomes replicate 1; Nippostrongylus brasiliensis; miRNA-Seq
library_construction_protocol: Three different biological replicates of EVs obtained from three different batches of worms were used. ODG fractions with a density between 1.07 and 1.09 (fractions containing pure EV samples after TRPS analysis) were pooled and excess Optiprep® solution was removed by buffer exchanging. miRNA were extracted using the mirVana miRNA Isolation Kit (ThermoFischer) according to the manufacturer's instructions. miRNA was prepared for sequencing using QIAseq miRNA library prepartion kit (Qiagen) according to manufacturer's instructions.