title: Whole genomes of Kuwaiti Individuals with Saudi Arabian ancestry (KWS2)
design: Prior to library preparation, frozen DNA stock was diluted to a working solution of 50 ng/µl as recommended by Illumina (CA, USA) and was quantified by agarose gel analysis. DNA sample was sheared to generate uniform size segments averaging around 400 bps by using Covaris E220 instrument (Covaris, Woburn, MA, USA) with the parameters of Duty Cycle 10%; Intensity 4; Cycles per Burst 200; and Time 55 seconds. Sheared DNA was used to prepare sequencing libraries using TruSeq DNA sample preparation and cBot Paired End (PE) cluster generation kits as per manufacturer’s protocols (Illumina, CA, USA). Libraries were loaded on a HiSeq 2000 flow cell for paired-end sequencing using the TruSeq SBS 200 cycles chemistry.