title: KM genome
design: Sequencing-grade genomic DNA was isolated using a Genomic-Tip 500/G kit (Qiagen Inc., Valencia, CA, USA) and suspended in 10 mM Tris-HCl (pH 8.5). Genomic DNA was sonicated in a Bioruptor (Diagenode SA, Liège, Belgium) for three cycles of 15 s on/90 s off at high power, to an average size of 450 bp. Library preparation was performed using a TruSeq DNA Sample Preparation kit (Illumina Inc., San Diego, CA, USA). Pair-end sequencing was performed on an Illumina Genome Analyzer IIx apparatus, generating 100-bp pair-end reads