title: H18-JA2-RUN4
design: Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen). mRNAs were isolated from 5  g of total RNA using the Magnosphere UltraPure mRNA purification kit (Takara), following manufacturer s instruction. The first-strand cDNA synthesis, reverse transcription and library amplification were performed with the SMARTer Stranded RNA-Seq kit (Clontech), using 8  l of mRNA following manufacturer s instruction. The enrichment step was carried out using 11 cycles of PCR. The pooled libraries (20 pM) were sequenced on an Illumina MiSeq in 6 consecutive runs (MiSeq reagent kit V3, 150 cycles) generating 75 base pairs (bp) paired-end reads.