title: immature fruit, asymptomatic
design: Leaf and fruit samples were collected from three types of orchard trees: symptomatic (SY) and apparently healthy (AH) in an infected orchard, and healthy trees from an HLB-free location (CO). A fourth sample type, “asymptomatic” (AS) tissues were collected from infected trees displaying symptoms elsewhere in the same tree, from the same location as the AH trees. Samples were collected in four tissues: Immature Fruits (IF) Mature Fruits (MF), Young Leaves (YL), and Mature Leaves (ML); four disease sample types from each of these, to yield 16 sample types total. A pool of 5 to 10 different leaves or fruits was collected from each of five different trees per treatment group, representing five biological replicates. RNA from the five biological replicates was equally pooled to 10 µg and then used to construct one cDNA library for each of the four HLB status categories for each tissue. cDNA library construction and high throughput sequencing. RNA from the five biological replicates was equally pooled to 10 µg and then used to construct one cDNA library for each of the four HLB status categories for each tissue. Each library was run as an independent lane on a Genome Analyzer II (Illumina, San Diego, CA) to obtain read lengths of up to 85 bp per paired end.