title: GSM1551817: mia50 d3/d5  batch3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: To extract RNA, frozen worms were re-suspended in ice-cold Trizol, zirconium beads, and glass beads (cat # 03961-1-103 and cat # 03961-1-104) in the ratio of 5:1:1 respectively, and disrupted in Precellys lysing system (6500 rpm, 3 x 10 s cycles) followed by chloroform extraction.The extracted RNA was precipitated and purified further using Qiagen RNAeasy Mini kit columns (cat # 74104). RNA was precipitated using isopropanol and washed once with 75% ethanol. 100 ng of total RNA per sample was processed using NuGEN Encore Complete DR RNA-Seq Prep Kit (NuGEN; San Carlos; CA, USA), as per manufacturer’s instructions RNA-seq to determine changes in expression proflies