title: Ion Torrent Proton sequencing; RNA-seq of Brachydpodium distachyon culms from control and BdBAHD01 RNAi plants
design: RNA-seq of Brachydpodium distachyon culms from control and BdBAHD01 RNAi plants
library_construction_protocol: Plants were grown in controlled environments as previously described Molinari et al. (2013) Frontiers in Plant Science 4: 50 Agrobacterium transformation of brachypodium distachyon Bd21 was performed as described Vogel & Hill (2008) Plant Cell Reports 27(3): 471-478. RNA was isolated as previously described Molinari et al. (2013) Frontiers in Plant Science 4: 50. RNA-seq libraries were constructed the Ion Total RNA-Seq Kit v2 (ThermoFisher Scientific) according to the supplied protocol.