title: Deep Sequencing of Zaire Ebolavirus Strain Mayinga Thp1 Cells 6hpi
design: Deep RNA sequencing of human Thp1 infected cells with Zaire                 Ebolavirus Mayinga Strain at multiplicity of infection of 3. RNA was extracted with                 TriZol, poly-A RNA purified with Invitrogen Dynal oligo dT magnetic beads and then                 RNA-Seq libraries created using NEB Next mRNA-Seq kit using 18 cycles of PCR.                 Samples sequenced on Illumina HiSeq 2500, using 100nt single read                 settings.