title: RNA-seq of Chlamydomonas reinhardtii cc503: A15
design: In order to understand the mechanism of cold response, we profiled and analyzed the transcriptome of C. reinhardtii from RNA-Seq data across 28 samples that spread across different time periods of normal condition (25oC), mild cold stress (15oC), severe cold stress (5oC), recoveries from two cold stresses (25oC) and cold acclimation from 15 to 5oC. We discovered a small proportion of genes (5% on average) are differentially expressed when comparing neighboring samples in time series. By comparing differential genes in two types of cold stress at the early stage, we found cold response at 5oC involves more genes and functions. By GO (gene ontology) enrichment analysis, we found more functions are associated with cold response at 5oC. We further validated conclusions by clustering and visualization of differential genes.