title: GSM3331232: rbm48-umu1 +/+ (WT) Rep2; Zea mays; RNA-Seq
library_construction_protocol: Endosperm tissue from 16-18 DAP normal and rbm48 kernels in the W22 genetic background was dissected and frozen in liquid nitrogen. Total RNA was isolate using TRIzol reagent followed by a clean up with the Qiagen Rneasy kit. Non-strand specific TruSeqTM (Illumina) cDNA libraries were prepared from 1 µg total RNA input with a 200 bp median insert length.