title: GSM2929095: Hepatocyte_5hmDIP; Mus musculus; MeDIP-Seq
library_construction_protocol: DNA was isolated from hepatocytes harvested from 20 day-old mice and sheared with the M220 Focused-ultrasonicatorTM (Covaris) using snap-cap tubes. Libraries were generated using NEBNext Ultra DNA Library Prep Kit (NEB E7645). Library DNA was denatured in 95C for 10 min. Denatured DNA was precipitate with 5hmc Ab (active motif 55010) and sequenced (50 bp single-end) on Next-seq (Illumina).