title: Illumina HiSeq 2000 sequencing; Single cell RNA sequencing of DDX4 antibody positive and negative ovarian tissue cells cultured under oogonial stem cell conditions
design: Single cell RNA sequencing of DDX4 antibody positive and negative ovarian tissue cells cultured under oogonial stem cell conditions
library_construction_protocol: Single cells were randomly picked by mouth pipetting with a finely pulled glass capillary and placed directly into lysis buffer containing 1 uL of RNase inhibitor and 19 uL of 0.2% (vol/vol) Triton X-100 solution (Sigma-Aldrich, Germany). The 8-strip PCR tubes containing cells were immediately frozen on dry ice and kept at -80C for downstream applications. Regarding spike-ins: 0.1uL of a 4*10^-6 dilution of the ERCC RNA spike-in mix 1 (Ambion) was added to each sample. na Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014