title: RNA-Seq of Salvia miltiorrhiza: WT Salvia miltiorrhiza after drought stress
design: After enrichment and purification with oligo(dT) paramagnetic beads, mRNAs were sheared into short fragments that were subsequently used as templates for first- and second-strand cDNA synthesis. AMPure XP beads were employed for purifying double-stranded cDNA, 3  ends were enzymatically repaired, polyadenylated, and ligated to adapters to select templates of different sizes, and the resulting cDNA libraries were subjected to PCR amplification and sequenced using an Illumina HiSeq2500 RNA sequencing instrument.