title: GSM3330339: A549-Treated-Replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were gently agitated on an orbital shaker at 80 revolutions per minute for 10 minutes. A lysis cocktail comprised of 10µL 1% β-mercaptoethanol, 20µL proteinase K, and 800µL RLT buffer, which was a component in the RNeasy Mini Kit (Qiagen), was homogenized with cells in each well. The RNA extraction was conducted according to instructions in the RNeasy Mini Kit. Additionally, a 30 minutes on-column DNase I treatment was performed.   DNA contamination was further minimized with TURBO™ DNase treatment. Next, RNA was purified with the Angencourt RNAClean XP Kit. RNA integrity was verified using the RNA ScreenTape with analysis on the Agilent 2200 TapeStation (Agilent). Using the Qubit™ dsDNA HS and RNA HS Assay Kits, total RNA was quantified fluorometrically via the Qubit™ Fluorometer 2.0 (Invitrogen). mRNA was purified with the Illumina® TruSeq® Stranded messenger RNA Sample Prep Kit