title: WGS: S. purpuratus: Coelemocytes
design: Cells were stored in RNALater were diluted with PBS and pelleted via centrifugation. Cells were subjected to proteinase K digestion. Total gDNA extracted with Phenol-Chloroform with RNAse treatment followed by re-extraction. Material was size fractioniated in such a way to allow the paired end reads to overlap and be fused into single, larger fragments