title: GSM1910382: FPS-SQS_1; Nicotiana tabacum; RNA-Seq
library_construction_protocol: Total RNA extracted from the third youngest leaf of each plant using miRNeasy kit (Qiagen) Poly(A)+ RNA was isolated using oligo dT beads (Life Technologies, Frederick, MD). RNA was fragmented for 7 minutes using Fragmentation Reagent (Life Technologies, Waltham, MA). mRNA-seq libraries were then generated using the Illumina mRNA-seq kit (illumina, San Diego, CA).