title: miRNA-seq of homo  sapiens: GMSCs-exosomes -1
design: Total RNA from exosomes was isolated using miRNeasy Serum/Plasma Kit (QIAGEN) according to the protocol. The amounts and integrity of total RNA were determined by Agilent 2100 Bioanalyzer (Applied Biosystems, Carlsbad, CA). RNA was concentrated by ethanol precipitation at -20C overnight after elimination of polyadenylated mRNA using oligo-dT dynabead. Small RNA library was prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina. Accurate quantification for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems, Inc., Woburn, MA). Each library was diluted to a final concentration of 2 nM and pooled equimolar prior to clustering. 150bp pair-end (PE150) sequencing was performed on all samples