title: GSM3860817: EXOSC4_CLIP; Homo sapiens; RIP-Seq
library_construction_protocol: HITS-CLIP was performed largely as described (Darnell, 2012), except that H1 cells were irradiated with 600 mJ/cm2 UV light (254 nm), 1 mM EDTA was added to PXL (PXL+E) to decrease exosome-mediated RNA degradation during the procedure, beads were washed 3 times with PXL + E, 3 times with 50 mM Tris-HCl pH 7.5, 400 mM NaCl, 1% NP-40, 1% deoxycholate, 0.1% SDS, 1 mM EDTA, 0.2 M urea, and 3 times with PNK buffer prior to using micrococcal nuclease to trim RNA tags.