title: GSM3398796: sRNAseq of F10 pedigree 2 spore 6, paf1-Q264Stop; Schizosaccharomyces pombe; miRNA-Seq
library_construction_protocol: Total RNA was isolated from exponentially growing cells using the hot phenol method (Leeds, 1991). The RNA was fractionated using RNeasy Midi columns (Qiagen) following the „RNA cleanup protocol“ provided by the manufacturer. The flow-through fraction was precipitated („small RNA“ fraction). 25 μg of the "small RNA" fraction were separated on a 17.5% PAGE and the 18 – 28 nt population was purified. The isolated small RNA populations were prepared for sequencing using the Illumina® TruSeq small RNA preparation protocol (Cat.# RS-930-1012).