title: GSM4142225: T5.WGS; Trichinella murrelli; OTHER
library_construction_protocol: DNA was extracted using the phenol chloroform extraction method. High molecular DNA from 12 Trichinella genotypes were extracted from freshly collected muscle larvae. Qubit Fluorometer dsDNA HS Kit (Invitrogen) was used to determine total DNA amounts, followed by measurement of integrity by by agarose gel electrophoresis and using a BioAnalyzer (2100, Agilent). Paired-end sequencing libraries with insert size of 300 bp were conducted on the HiSeq 2,500 platform, according to instructions provided by Illumina.