title: GSM1429751: EM-mRNA-1; Echinococcus multilocularis; RNA-Seq
library_construction_protocol: Total RNA from the parasites was purified with Trizol reagent and contaminating gemomic DNA was removed by the RNase-Free DNase Set. Polyadenylated RNA  were isolated from total RNA. The mRNA was interrupted into short fragments by adding fragmentation buffer as described (Illumina RNA-seq kit, part no. 1004898). Taking these short fragments as templates, random hexamer primers were used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I, respectively. Short fragments were purified and double strand cDNAs were end-repaired according to manufacturer-recommended protocols (https://icom.illumina.com/), and then were connected with Illumina adapters (Illumina RNA-seq kit, part no. 1004898). The fragments were first amplified by PCR. Purified cDNA fragments were pooled and indexed and were loaded onto one lane of an Illumina HiSeq™ 2000 instrument. Pair-end 100 sequencing cycles were carried out.