title: GSM4085635: wt-3051_S2; Mus musculus; RNA-Seq
library_construction_protocol: KI and WT littermate mice at 5-27 weeks of age were euthanized via CO2 asphyxiation, and dissections of the cochlea, lung, and kidney were immediately frozen in liquid nitrogen. Tissue was pulverized with a Covaris CPO2 CryoPrep Automated Dry Pulverizer (Covaris, Woburn, MA) and RNA was extracted with Trizol (Invitrogen). For RNA-seq, total RNA from cochleae was reverse transcribed with random primers after ribo-depletion using a TruSeq library kit (15031048 Rev. C, Illumina, San Diego, CA), and 2x93bp sequenced on an Illumina HiSeq1500 instrument.