title: RNA-seq of Myxococcus xanthus: development 24 hr
design: Total cellular RNA was isolated using the RNAqueous-Micro kit. gDNA contamination was removed with DNaseI. rRNA was depleated via hybridization to 76 short synthetic DNA oligonucleotides and RNaseH digestion. Libraries were prepared with the KAPA Stranded RNA-seq Library Preparation kit.