title: GSM4367371: BMDMs, LPS3; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow cells were flushed from tibias and femurs with cold Dulbecco's modified Eagle's medium (DMEM) and were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 10 ng/ml macrophage colony-stimulating factor (M-CSF, PeproTech) for 6 days to generate BMDMs. Then treated with LPS and Kir2.1 inbibitor ML133 for 6 hours.And total RNA were extracted for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols