title: RAD-seq of Nelumbo nucifera: Chinese Tai-zi lotus hybridize Thailand Chiang Mai wild F2 individual 062
design: The genomic DNA were digested with the TaqI restriction endonuclease, ligated to specific P1 adapters that containing unique barcodes, pooled and randomly sheared. DNA fragments with length between 400 and 600 bp were isolated via agarose gel electrophoresis. P2 paired-end adapters were subsequently ligated to the isolated DNA fragments for sequencing.