title: GSM3444142: LiverpE2_ERa_ChIPseq_2; Mus musculus; ChIP-Seq
library_construction_protocol: Tissue were removed and flash freezed in liquid nitrogen. RNA were extracted using the direct-zol RNA Miniprep kit (R2052, Zymo Research). For ChIP experiment, nuclei were first isolated and sonicated, then a anti-ERα antibody (sc543, Santa Cruz Biotechnology) was used to purified ERα enrichied chromatin. RNA-seq and ChIP-seq libraries were prepared for sequencing using standard Illumina protocols. Shortly, RNA-seq librairies were prepared following Baby, V. et al. (PMID: 28893065) procedure. ChIP-seq librairies were produced as proviously describe in Bianco, S. et al. (PMID: 25435372).