title: GSM4086269: henn-1_gonads_2_sRNA_setA; Caenorhabditis elegans; OTHER
library_construction_protocol: Small RNAs (~16-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase to facilitate capture of 22G-RNAs and/or oxidized with sodium periodate to distinguish methylated small RNAs (as indicated in the molecule section). Libraries were then prepared according to the NEBNext® Multiplex Small RNA kit protocol. PCR products (~134-146 bp) were size selected from 10% polyacrylamide gels Added to SAMPLES section. Added to SAMPLES section.