title: GSM3330180: 14-077N15_LIVER; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from sections of frozen tissue using RNA STAT-60 (Tel-Test) while incorporating Phase Lock Gel Heavy tubes (5 Prime) into the protocol to better facilitate phase separation.   RNA yield and purity was obtained on a NanoDrop 2000 (Thermo Fisher Scientific).  RNA Integrity was evaluated on a 2100 Bioanalyzer (Agilent Technologies). RNA-seq libraries were constructed from 1 ug total RNA using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit according to the manufacturer's protocol. Barcoded libraries were pooled and sequenced six per lane on the Illumina HiSeq 2500 generating 50 bp paired end reads.