title: IGH DNA NGS of Homo sapiens: Peripheral B cells
design: IGH VDJ gene rearrangements of all IGHV subgroups were amplified using the LymphoTrack IGH FR1 Assay kit (LymphoTrack, InvivoScribe). Amplified DNA was processed for HTSeq according to the LymphoTrack protocol. DNA was amplified with a one-step multiplex PCR for 29 cycles using subgroup-specific IGH framework region 1 primers and IGHJ primers, incorporating platform specific adaptors. PCR products were purified and pair-end sequenced using the MiSeq v3 reagent kit .