title: GSM3104498: DICER1*_+DOX_1_c1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using miRNeasy Mini Kit (Qiagen, Cat. No. 217004). After ribogreen quantification and quality control on bioAnalyser,  500ng of total RNA underwent library preparation using the Truseq Stranded Total RNA library preparation chemistry (Illumina), with 6 cycles of PCR. Samples were barcoded and run on a Hiseq 2500 1T in a 50bp/50bp Paired end run, using the TruSeq SBS Kit v3 (Illumina).  An average of 50 million paired reads were generated per sample and the percent of mRNA bases was 72% on average.