title: GSM3872527: RTK17.1_O2-excess_N-sufficient (NH4+) [RTK171120716]; Methylacidiphilum sp. RTK17.1; RNA-Seq
library_construction_protocol: isolation of mRNA was performed using the RNeasy Mini kit (Qiagen) according to the manufacturer's protocol. Following total RNA extraction, ribosomal RNAs were removed using the Ribo-Zero rRNA removal kit (bacteria) and the quality of the remaining RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent). Ribo-Zero User Guide, TruSeq Stranded Total RNA prep