title: GSM2850560: HD6_AD; Homo sapiens; RNA-Seq
library_construction_protocol: Qiazol-Chloroform extraction performed, and RNA purified by miRNeasy mini kit according to the manufacturers protocol. (Qiagen, Valencia, MA) Amplified cDNA was synthesized from RNA extracted from captured extracellular vesicle products using the SMARTer Ultra Low Input RNA Kit for Sequencing - v4 kit (Clontech Laboratories). PCR amplification following second strand synthesis was run for 18 cycles. 1 ng of amplified cDNA was loaded into the Nextera XT kit. Normalization was done using the KAPA SYBR® FAST Universal qPCR Kit (Kapa Biosystems) rather than the bead-based normalization in the Nextera XT kit.