title: Panel capture-enriched DNA of Homo sapiens: adult healthy circulating cell-free DNA
design: Libraries were prepared from 10 ng of circulating cell-free DNA isolated from plasma. Template DNA underwent end-repair and A-tailing followed by ligation of singleton adapters (5  at 15 _M concentration added to each reaction) using the NEBNextltra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA) following the manufacturer instructions. Samples then underwent PCR amplification using the KAPA Library Amplification Kit (Roche, Indianapolis, IN) following the manufacturer instructions. Samples were amplified using 5  of 20  P5/P7 primers and 20  indexing primers, respectively. Amplified DNA libraries underwent panel capture enrichment using a custom designed IDT Xgen capture probe set (118 genes, 124 kb) followed by paired-end sequencing (2x125 bp) on a HiSeq 2500 (Illumina, San Diego, DA).
library_name: Ctrl_6_ccfDNA_Singleton