title: Blk2_O3
design: cDNA library preparation was done using the Illumnina TruSeq Sample Prep kits (Illumina Inc. San Diego, CA, USA).  cDNA libraries were made for each biological replicate per treatment (n =3 in 2011, n=4 in 2012).  Library fragments were barcoded and multiplexed for sequencing to obtain 100nt single-end reads.  In 2011, 8 samples were sequenced across 2 lanes, generating ~42-49 million reads per sample.  In 2012, 16 samples were sequenced across 4 lanes, generating ~ 40-53 million reads per sample.  Sequencing was done at the Biotechnology Center using the Illumina Genome HiSeq 2000 platform (www.igb.illinois.edu/biotech/htdna/services-equipment/illumina).
library_name: Blk2_O3