title: GSM2358036: WT biological replicate #1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs purified from mouse embryonic stem cells using RNeasy® Plus Mini Kit (Qiagen) were treated with DNase I to remove residual genomic DNA. RNA enriched by oligo-dT magnetic beads, and sonicated (Bioruptor®, Diagenode) into roughly 200 bp fragments. cDNAs were synthesized with random hexamer-primers, purified by silicon-coated magnetic beads, blunted in the ends, added with a single adenine at 3'-end, ligated with sequencing adaptors and PCR-amplified. The quality and quantity of the resulting library were determined by Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System, respectively. The library products were sequenced by Illumina HiSeqTM 2000.
attributes.tag: GEO Accession
attributes.value: GSM2358036