title: U3 RNAseq
design: RNA was extracted urine pools using Trizol LS Reagent (InvitroGen). The material was then amplified by a non-bias random-primer protocol (Cantalupo et al. mBio. 2011;2(5). Epub 2011/10/06. doi: 10.1128/mBio.00180-11. PubMed PMID: 21972239; PubMed Central PMCID: PMCPMC3187576). Resulting samples were submitted to the Genomics Research Core of the University of Pittsburgh (https://www.genetics.pitt.edu/) for library construction and Illumina sequencing.
library_name: U3_RNA