title: Knock-on community impacts of a novel vector: Spillover of emerging DWV-B from Varroa-infested honeybees to wild bumblebees
design: We pooled 1000ng of RNA from each of 30 A. mellifera and 60 B. terrestris, from two Varroa-free island sites (Ushant and the Isle of Man) and their paired Varroa-present mainland sites (Le Conquet and Liverpool), to create eight populations for SMRT sequencing. Full-length cDNA libraries were prepared using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin System. The PacBio Template Prep Kit was used to generate SMRTbell libraries, which were sequenced on the PacBio System by Exeter Sequencing Service.
library_name: Ba1