title: GSM1976282: OtA8537; Mus musculus; RNA-Seq
library_construction_protocol: Lymph nodes were harvested from mice 40 min after SEA or vehicle inhalation and cruched into single cell suspensions. The cells were stained with live/dead stain and anti-Vβ3 and anti-Vβ14 antibodies and sorted by FACS Aria II. Total RNA was obtained from the sorted cell lysates using RNeasy Mini kit (Qiagen) and sent to Otogenetics (Norcross, GA) for analysis. All samples passed the required quality control and underwent ultra low RNA input polyA cDNA preparation. Low input RNA library were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM1976282