title: GSM2935897: B6ES_FAIRE_Rep1; Mus musculus; FAIRE-seq
library_construction_protocol: Trypsinised feeder-free cells (1×10^6) were collected and fixed with 1% formaldehyde at room temperature. Crosslinking reaction was quenched with 125 mM glycine for 5 min. The cells were resuspended in SDS lysis buffer (ChIP Reagent, Nippon Gene Co., Ltd.), and the lysate was sonicated to fragment chromatin using a Covaris S220 (Covaris, Woburn, MA, USA). DNA was purified from sonicated lysate using phenol-chloroform (Sigma #P3803 phenol, chloroform, and isoamyl alcohol 25:24:1 saturated with 10 mM Tris at pH 8.0, 1 mM EDTA), and ethanol-precipitated. Sequencing libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos from Illumina (New England BioLabs Inc., Ipswich, MA, USA) according to the manufacturer's instructions. FAIRE DNA (1 ng) was subjected to end repair, dA-tailing, and adaptor-ligation, and amplified by 9 cycles of PCR. The formaldehyde-assisted isolation of regulatory elements combined with the high-throughput sequencing (FAIRE-seq)
attributes.tag: GEO Accession
attributes.value: GSM2935897