title: GSM2696366: BMM inf; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from the uninfected or infected BMMs were isolated in accordance with the Qiagen RNeasy Mini kit “Animal Cells I” protocol (Qiagen). DNA was removed by using the “On-Column DNase Digestion” protocol. The Ribo-Zero rRNA removal kit (Epicentre, USA) was used for ribosomal RNA depletion according to manufacturer’s instructions. Libraries for Illumina sequencing were made with the TruSeq Stranded mRNA sample prep kit (Illumina, USA) following the manufacturer’s protocol. RNA sequencing was performed on the Illumina HiSeq 2500 platform using single-end 50 bp sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2696366