title: GSM2693422: Human_Hs2_V1C; Homo sapiens; RNA-Seq
library_construction_protocol: Brain dissection was performed from either fresh specimens or frozen tissue slabs by the same person to ensure sampling consistency between specimens. About 100 mg of dissected tissues were used and total RNA were extracted using RNeasy Plus Universal Kit (Qiagen). Quality and quantity measurements of extracted RNA were performed using NanoDrop (Thermo Fisher Scientific) and Qubit Fluorometer (Thermo Fisher Scientific), respectively, and the RNA Integrity Number (RIN) were determined using Bioanalyzer RNA 6000 Nano Kit (Agilent). Sequencing libraries were prepared using the NEBNext mRNA Library Prep Kit for non-directional libraries and the NEBNext Ultra Directional RNA Library Prep Kit for directional libraries (New England BioLabs) according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2693422