title: Illumina HiSeq 2500 paired end sequencing; RNA-Seq of ALDH positive, ALDH negative and unsorted cells from patient-derived 3D in-vitro models of colon cancer
design: RNA-Seq of ALDH positive, ALDH negative and unsorted cells from patient-derived 3D in-vitro models of colon cancer
library_name: 46_302-CB-M-MF_VITRO_ALDH-POS_CCGTCC_p
library_construction_protocol: 3D in vitro models of patient derived colon cancer tumors were processed to single cells, labelled for ALDH activity using the Aldefluor Assay and separated in ALDH- ad ALDH+ cell fractions Cells were lysed in RLTplus buffer and RNA was extracted using Qiagen RNeasy Mini Kit. RNA quality was assessed prior to sequencing TruSeq RNA V2 library construction protocol from Illumina
attributes.tag: Experimental Factor: aldehyde dehydrogenase positive
attributes.value: fraction