title: GSM2695481: sbTAC_Rep1_ATAC; Mus musculus; ATAC-seq
library_construction_protocol: Bulge hair follicle stem cells (GFP+/CD34+/Itga6+/Itgb1+) and hair germ cells (GFP+/CD34-/Itga6+/Itgb1+) at the second telogen and basal TACs e (GFP-/CD34-/Itgb1high/Pcad+/SCA1-) and suprabasal TACs (GFPlow/Itga6-/CD34-) are FACS-purified and immediately sujected to ATAC-seq library generation. FACS-sorted cells were pelleted and incubated with cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). After removing lysis buffer by centrifugation, samples were  subjected to a transposition reaction at 37°C for 30 min with 10μL transposase enzyme (Illumina Nextera DNA Preparation Kit). Transposed DNA was purified using QIAGEN MiniElute PCR purification kit and PCR amplified with 10-15 cycles. Library concentration and quality was assayed by D1000 Tape Station (Agilent) prior to sequencing. The samples were sequenced on Illumina HiSeq 2000 using a 50bp paired-end-reads setting.
attributes.tag: GEO Accession
attributes.value: GSM2695481