title: GSM1976367: E14.5mouseCerebralCortexTbr2EGFP-_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from sorted cells were extracted using RNeazy Mini Kit (Qiagen) and treated with Turbo DNase (Ambion). The integrity of DNase-treated total RNA was measured by RNA Nano chips on Bioanalyzer (Agilent), and the RIN for all samples were above 9.0. 500-2000ng total RNA was treated with Ribo-Zero Gold (Epicentre) to remove ribosomal RNAs, and mRNA was further enriched using Oligo(dT)25 Dynabeads (Life Tech). RNA-Seq libraries were prepared and barcoded using ligation-based directional RNA-Seq kit (Bioo Scientific), and sequenced on Illumina HiSeq2000 using single end 50 bp reactions.
attributes.tag: GEO Accession
attributes.value: GSM1976367