title: GSM3573649: Control_EB scRNA-seq [D]; Homo sapiens; RNA-Seq
library_construction_protocol: Within each GEM reaction vesicle, a single cell is lysed, the Gel Bead is dissolved to free the identically barcoded RT oligonucleotides into solution, and reverse transcription of polyadenylated mRNA occurs. Single cells were collected from two independent EB differentiation experiments from day 21 EBs (control and nicotine-exposed) and dissociated using ACCUTASE™ (STEMCELL Technologies). They were prepared for the single-cell library separately. In brief, cells were washed with 1X DPBS (GIBCO) three times, strainer-filtered, and re-suspended in 0.04% BSA. Viable single cells were loaded to GemCode Instrument (10X Genomics, Pleasanton, CA) to generate single-cell barcoded droplets (GEMs) using the 10X Single Cell 3' v2 chemistry and 10X Chromium system as per the manufacturer's protocol. R2: biological, R1: technical (16bp 10x barcode+10bp UMI), I1: index
attributes.tag: GEO Accession
attributes.value: GSM3573649