title: GSM3638986: RNA-seq Control1-A184; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs of sciatic nerves were isolated using the RNeasy Kit (Qiagen) including a DNAse treatment step. RNA quality was assessed using RNA Screen Tape 6000 Pico LabChips with the Tape Station (Agilent Technologies) and the RNA concentration was measured by spectrophometry using Xpose. RNA-seq libraries were prepared starting from 60 ng of total RNA using the Universal Plus mRNA-Seq (Nugen) as recommended by the manufacturer. The oriented cDNA produced from the poly-A+ fraction were PCR amplified (15 cycles). An equimolar pool of the final indexed RNA-Seq libraries was sequenced on an Illumina HiSeq2500 (paired-end reads 130 bases + 130 bases). A mean of 124 millions of passing filter paired-end reads was produced per library sample (with a minimum of 103 million and a maximum 158 millions).
attributes.tag: GEO Accession
attributes.value: GSM3638986