title: GSM2174878: BJATAClib4; Homo sapiens; OTHER
library_construction_protocol: Simultaneously lysed and transposed with 0.01% w/v digitonin in ATAC-seq transposition reaction. Libraries were constructed by transposition of permeabilized cells with Illumina Nextera Tn5 transposase that inserts short sequencing adapters.  The transposed DNA was purified using a Qiagen MinElute PCR cleanup kit and overhangs were extended by 5 minute incubation at 72˚C, followed by PCR with barcoding primers to introduce the remaining adapter sequence. i7 barcodes were used for multiplexing (TAAGGCGA, CGTACTAG, AGGCAGAA, TCCTGAGC, GGACTCCT, TAGGCATG, CGAGGCTG, AAGAGGCA).
attributes.tag: GEO Accession
attributes.value: GSM2174878