title: RNA-Seq of Halocynthia roretzi : Whole Embryo WT rep2 Stage 8 (64-cell)
design: For each sample, 200-300 embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Truseq Strand-specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
library_name: Harore-WE-WT-BR2-64c