title: GSM3334078: WT_-1.5kb_rep2; Mus musculus; Bisulfite-Seq
library_construction_protocol: Total genomic DNA from in vitro induced Treg cells at day 7 in culture was isolated using Accuprep Genomic DNA Extraction Kit (Bioneer). Bisulfite conversion of DNA was performed with MethylEasy Xceed kit (Human Genetic Signature). PCR amplicon from loci of interest was generated from this treated DNA. After electrophoresis to confirm the size these PCR products were then purified from agarose gel using HiGene Gel and PCR purification system (Biofact). Library was contructed using manufacture's protocol accompanying the kit TruSeq Nano DNA LT Library Preparation Kit - Set A : FC-121-4001,  Nextera DNA Library Prep Kit (24 samples) : FC-121-1030, Nextera Index Kit (24 indexes, 96 samples) : FC-121-1011
attributes.tag: GEO Accession
attributes.value: GSM3334078