title: GSM4087920: XXSry_2A; Mus musculus; RNA-Seq
library_construction_protocol: Auxillary, brahcial, and inguinal draning lymph nodes were collected and processed into single cell suspensions. The cells were cultured at a concentration of 1x10^6 in complete RPMI medium with MOG 35-55 (25 ug/mL) and IL-12 (20 ng/mL) for 36 hours. After culture, cells were sorted for CD4+ T cells by magnetic negative selection using EasySep Mouse CD4+ T cell isolation kit (STEMCELL). RNA was isolated from CD4+ T cells using the Direct-zol™ RNA MiniPrep Plus kit (Zymo Research). Double-stranded cDNA and the sequencing library were made using the Kapa RNA Kit Full kit.
attributes.tag: GEO Accession
attributes.value: GSM4087920