title: 2b-RAD reads of Ussuri catfish
design: Genomic DNA of each individual was firstly digested using the restriction enzyme BcgI, then digested DNA was ligated with adapters and  amplified using Phusion high-fidelity DNA polymerase. Amplicons were separated by electrophoresis in 8% polyacrylamide gels and purified, and then a final library was made by mixing equal amount of products from all libraries and sequenced on a lane at the Illumina HiSeq2500 platform.
library_name: 2b-RAD