title: RNA-Seq of Gallus gallus: male jejunum
design: Equal amounts of RNA (i.e. 500 ng) from 3 different chicks within the same experimental group were pooled together, so that we obtained a total of four RNA pools (i.e. replicates) for each treatments (diet O and diet OY ). Thus, RNA-seq data are representative of a total of 24 animals (12 per group). A total of 8 tagged RNA-seq libraries were therefore prepared from RNA pools using Agilent's SureSelect Strand Specific RNA Library Preparation Kit, following manufacturer's instruction. Briefly, poly(A) mRNA was purified from 1.5  ug of total extracted RNA and fragmented using an RNA-seq Fragmentation Mix. First-strand and second-strand cDNA were synthetized and end-repaired. Adenylation of cDNA 3' ends and adaptor ligation were performed. Twelve cycles of PCR were used to amplify and index the adaptor-ligated cDNA library, and the PCR products were purified and size selected using the SPRIselect reagent kit.
library_name: AMML-1-10