title: miRNA-Seq of human CLL cells
design: Peripheral blood from chemonaive CLL patients participating in a previously reported clinical trial (RevliRit trial, ClinicalTrials.gov Identifier: NCT00738829 and NCT01703364) with first line treatment with lenalidomide in combination with fludarabine and rituximab was collected prior treatment start and CLL cells were obtained by density gradient centrifugation and B-CLL Cell Isolation Kit (Miltenyi Biotec). Cell purity was >90% in all samples. Total RNA was purified using RNeasy Mini Kit (Qiagen), respectively. cDNA library with an insert size of 19-30 bp was prepared with chemistry v3.0 and sequencing was performed on a HiSeq 2000.
library_name: 016_CLLRNA_miRNASeq