title: GSM4452560: 3D_Cardiac_Organoid_CM_Day27_WTC-quantseq [CM_Organoid_D27-114970.5-quantseq]; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated with the RNeasy Mini Kit (Qiagen, #74104). Generation of the RNA-seq libraries was performed according to manufacturer's instructions with QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen GmbH, #015). After the preparation of the libraries, samples were checked for an adequate size distribution with a fragment analyzer (Advanced Analytical Technologies, Inc) and were submitted to the Vienna Biocenter Core Facilities (VBCF) Next-Generation-Sequencing (NGS) facility for sequencing. For Smart-Seq2 analysis, 400 cells were sorted into lysis buffer and stored at -80C until further processing. Samples were QC'd/libraries prepared and sequenced by the VBCF NGS facility using a home-made Smart-Seq2 kit.
attributes.tag: GEO Accession
attributes.value: GSM4452560