title: RNA from hot spring microbial mats
design: The V3-V4 regions of the 16S rRNA gene were amplified from the extracted RNA using the PRK341F/PRK806R primers (Yu et al., 2005). Equal amounts of amplicons were pooled for each sample into a normalized library, and the sequencing was performed on a MiSeq platform (Illumina, USA) using V3 sequencing chemistry with 300 bp paired-end reads.
library_name: HS_RNA