title: GSM2694052: cuticulosomes (pigeon 513); Columba livia; RNA-Seq
library_construction_protocol: RNA extractions of 30 isolated hair cells with and without cuticulosomes from 9 birds respectively (18 samples in total) were performed using the TRIZOL/Chloroform method and the RNA Clean and Concentrator-5 Kit (Zymo Research, R1015) according to manufacturer's protocol. cDNA libraries were prepared employing a full length RNA-seq from single cells protocol (Picelli et al. 2014). Briefly, this method involved preamplification of reverse transcribed mRNA using a template switching oligo followed by fragmentation and adaptor ligation using a hyperactive derivative of the Tn5 transposase from the Nextera XT DNA Sample Preparation Kit (Illumina, FC-131-1096). Adapter-ligated DNA fragments were amplified by PCR using the Nextera XT DNA Sample Preparation Kit and the Nextera XT 24-index Kit (Illumina, cat. no: FC-131-1001). Sequencing was performed on an Illumina HiSeqV4 system with 50 base pair single-end reads using the True-Seq dual-index sequencing primers for single read runs (Illumina, FC-121-1003).
attributes.tag: GEO Accession
attributes.value: GSM2694052