title: GSM4200631: 133WTBLA; Mus musculus; RNA-Seq
library_construction_protocol: Brains were rapidly extracted and frozen in a dry ice bath containing isopentame. 300 micron thick sections containing the basolateral amygdala (BLA) and Nucleus Accumbens (NAc) was cut on the cryostat and brain regions were punched using a micropunch. RNA was extracted using the Magmax-96 kit from Ambion. DNA contamination was removed by DNAse treatment. RNAseq libraries were prepared for sequencing using standard illumina protocols using the NextSeq 500/550 High Output kit, Illumina Inc., San Diego, CA polyA enriched RNAs (MicroPolyA purification kit, Life Technologies, Carlsbad, CA) were used for library construction
attributes.tag: GEO Accession
attributes.value: GSM4200631