title: GSM3874933: RHB7014; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque (GE Healthcare) density gradient centrifugation of whole blood form a human donor Single cell cDNA libraries were made using the SMARTSeq v2 protocol (Picelli et al., 2014) with the following modification: Use of 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). All samples were subjected to an indexed paired-end sequencing run of 2x151 cycles on an Illumina HiSeq 4000 system (Illumina, San Diego, CA, USA) (300 samples/lane). Single Cell RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM3874933