title: GSM2944073: RRBS_9_3; Homo sapiens; Bisulfite-Seq
library_construction_protocol: genomic DNA was extracted from the sperm cells of 12 men before and after the exercise intervention, as well as after the detraining period, with the Nucleon™ BACC Genomic DNA Extraction Kit (GE Healthcare, Life Sciences). The protocol was modified for processing of sperm, according to the manufacturer's recommendations. 200ng of genomic DNA was digested with 40U of MspI enzymes (New England Biolabs) and ligated to TruSeq (Illumina) sequencing adaptors. Bisulfite conversion was conducted once with the EpiTect bisulfite kit (Qiagen) in accordance with the manufacturer's instructions, and the converted DNA was amplified by PCR and sequenced
attributes.tag: GEO Accession
attributes.value: GSM2944073