title: GSM4200574: 14d Rap1_ChIPSeq biological repeat 1; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Cells were lysed with 300 ml acid washed glass beads (425-600 mm) by vigorous agitation for 30 s in a BeadRuptor2 followed by 30 s on ice, repeated 7 times. Sufficient lysis (90%) was confirmed by light microscopy. The lysate was subsequently sonicated at 4°C to obtain an average DNA fragment length of 300 bp. Samples were centrifuged and the supernatant, containing sheared chromatin, recovered. Libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4200574