title: GSM2694832: 3_24E; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from TRIzol Reagent-lysed ARPE-19 cells, according to the manufacturer’s instructions, and stored at -80 °C ahead of use for RNA-seq. RNA concentration was determined by spectrophotometry on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific-Invitrogen) for RNA-seq. RNA integrity was confirmed on the 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). Total RNA was depleted of ribosomal RNA and converted to cDNA libraries, using the TruSeq Stranded Total RNA kit with Ribo-Zero Gold (Illumina, San Diego, CA), strictly in accordance with the manufacturer’s instructions. RNA samples were diluted to 1.3 pM ahead of sequencing on the Illumina NextSeq 500, which was performed with NextSeq 500/550 High Output Kits (Illumina) for 2 x 75 cycles of sequencing. The PhiX Control v3 library (Illumina) was used as a sequencing control.
attributes.tag: GEO Accession
attributes.value: GSM2694832