title: GSM3400723: TRF2 replicate 3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Neural stem and progenitor cells were sorted directly into  TRIzol, homogenized with a pestle and then RNA was extracted according to the manufacturer's instructions, using Glycoblue 500mg/ml (Ambion, AM9516) as a co-precipitant We used SMART-seq v4 ultra-low input kit (Clontech, 634889) to generate double-stranded cDNA for each replicate, using 1ng of total RNA as input. 1ng of the resulting cDNA, amplified with 11 cycles of PCR in the cDNA amplification step, was used to prepare sequencing libraries with the Nextera XT kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3400723