title: Bloom #1 - EXP
design: Preparation of small RNA libraries was performed with the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer instructions. Briefly, 1 ?g of total RNA was ligated with adapters at 3’ and 5’ ends, without any size fractionation. Adapter-ligated RNA was reverse transcribed with SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA), then PCR-amplified (15 cycles). Samples were barcoded using 15 variants of the reverse primer provided with the kit. Libraries were pooled together and then purified on a 6% TBE PAGE gel after electrophoresis. Libraries quality and concentration were evaluated with the Agilent 2100 Bioanalyzer DNA1000 assay. The obtained cDNAs were sequenced using the Illumina Genome Analyzer IIx.
library_name: Bloom 1 LIB