title: GSM2943941: Quiescent_rep1_GL1_1; Mus musculus; OTHER
library_construction_protocol: For TRAP-seq, livers were prepared as previously described (Heiman et al. 2014). Briefly, liver homogenates were incubated with GFP-conjugated magnetic beads to affinity purify GFP-tagged ribosomal subunit L10A along with the attached translating mRNAs. RNAs were then isolated with the Absolutely RNA Nanoprep Kit (#400753, Agilent). For ATAC-seq, hepatocyte nuclei were prepared as previously described (Fang et al. 2014) and GFP-positive nuclei were labeled with Alexa Fluor 647 anti-GFP antibody (#338006, clone FM264G, 1:25, BioLegend) and sorted using BD AriaII. For TRAP-seq, mRNAs were isolated with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (#E7490, NEB) followed by cDNA library construction with the NEBNext® Ultra RNA Library Prep Kit for Illumina® (#E7530S, NEB). For ATAC-seq, nueclei were tagmented and PCR amplified according to previously published ATAC-seq protocol (Ackermann et al. 2016).
attributes.tag: GEO Accession
attributes.value: GSM2943941