title: GSM2696100: inDrop sequencing of WT sample (animal #2); Danio rerio; RNA-Seq
library_construction_protocol: RNA from individual cells were reverse-transcribed, barcoded, and unique transcripts are imprinted with UMI, all within the droplet. RNA from 1,500 cells of each animal were pooled and subsequently processed together. cDNA products were pre-amplified to produce a cDNA library compatible with the Illumina Nextseq platform. Three sequencing runs were completed using the Nextseq 500 High Output V2 kit (75 cycles) (Illumina) on a NextSeq 500 platform (Illumina). Zebrafish cells are isolated from the kidney marrow into single-cell suspensions, and treated with 15% Optiprep (Sigma). Single cells are microfluidically sorted into droplets containing reverse-transcription and barcoding reagents.  This method is outlined in Klein et al. (PMID 26000487) and Zilionis et al. (PMID 27929523).
attributes.tag: GEO Accession
attributes.value: GSM2696100