title: Genome of Rhizobium sp. BUS002: Western Kenya isolate
design: Total amount of 1.0g DNA was used as input material. Sequencing libraries were generated using NEBNext DNA Library Prep Kit and indices were added to each sample. The genomic DNA was randomly fragmented to a size of 350bp by shearing, then DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system) and resulting libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR.
library_name: FDMS202328829-1a