title: GSM2935046: TRMT2A_HEK293_iCLIP_rep2; Homo sapiens; RIP-Seq
library_construction_protocol: iCLIP was performed as previously described using a UV-crosslinking based method (Konig et al. 2012). The TRMT2A antibody used for immunoprecipitation was as for FICC-Seq. Libraries containing in-line barcodes were sequenced on the Illumina HiSeq4000 platform. Cells were harvested by washing in Phosphate Buffered Saline (PBS) and then incubating with Trypsin-EDTA followed by further washing of detached cells in PBS.
attributes.tag: GEO Accession
attributes.value: GSM2935046