title: Bacterial pool 2
design: Bacteria were grown until exponential phase in LB broth. DNA was isolated from the bacteria with the DNeasy kit (QIAGEN) following the instructions of the manufacturer. Yield of double-stranded DNA was quantified with the Qubit spectrofluorimeter (Invitrogen). DNA from eight bacteria were mixed with human background DNA in the following proportions: Staphylococcus epidermidis 0.195 ng, Proteus mirabilis 1.95 ng, Bacillus subtilis 1.95 ng, Klebsiella pneumoniae 4 ng, Aeromonas hydrophila 1.95 ng, Pseudomonas putida 1.95 ng, Pandoraea apista 4 ng, and Burkholderia cepacia 4 ng.
library_name: Experiment2