title: GSM4200020: AK86p2-SPH3d; Homo sapiens; RNA-Seq
library_construction_protocol: hKEpC were thawed and grown as 2D monolayers for 5-7 days. Upon reaching confluency of 80-100%, the cells were harvested and seeded on poly (2-hydroxyethylmethacrylate) (poly-HEMA; Sigma-Aldrich)-precoated plates, in serum free medium (SFM), at a concentration of 5.5–13x104 cells/mL. SFM was comprised of N2 medium (Biological Industries) supplemented with 1% Pen-strep 100M, 1% L-glutamine, 0.4% B27 supplement (Gibco), 4µg/ml heparin sodium (Intramed), 1% non-essential amino acids, 1% sodium pyruvate, 0.2% CD Lipid concentrate (all purchased from Invitrogen), 2.4mg/ml glucose, 0.4mg/ml transferrin, 10mg/ml insulin, 38.66µg/ml putrescine, 0.04% sodium selenite, 12.6µg/ml progesterone (all from Sigma-Aldrich), 10ng/ml FGF and 20ng/ml EGF. TruSeq mRNA-Seq library kit (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM4200020