title: "metabarcoding of complex plant food product: tea, spice or herbal remedy": Sample plant_food_product_P9
design: "The two-step PCR method was used for library preparation, in a way similar to protocol in work of [Speranskaya et al., 2018], but with new optimized primers that selectively anneal to 18S and 5.8S sequences of plants, excluding fungi. Encyclo Plus PCR kit (Evrogen, Russia) was used for nrITS1 amplification. Illumina libraries were sequenced on the MiSeq with MiSeq Reagent Kit v2 for 500 cycles with 251 + 251 cycles setting."
library_name: P9-3-Enc