title: Simultaneous Emergence of Multidrug-Resistant Candida auris on 3 Continents Confirmed by Whole-Genome Sequencing and Epidemiological Analyses
design: Genomic DNAs were sheared using focused ultrasonication (Covaris, Inc., Woburn, MA) and size selected using Ampure magnetic beads (Beckman Coulter, Indianapolis, IN). Indexed adapters were ligated to DNA fragments and libraries were purified and PCR amplified. Finished libraries were analyzed for size distribution using a Fragment Analyzer capillary electrophoresis system (Advanced Analytical, Ankeny, IA) and concentrations measured by Picogreen assay (Molecular Probes Eugene, OR). After denaturing, the paired-end libraries were diluted and loaded onto Illumina (San Diego, CA) flowcells for cluster formation. Clusters were then sequenced on an Illumina HiSeq 2500 using TruSeq SBS chemistry producing paired-end reads of 100 basepairs. Post-run processing and de-multiplexing was performed using CASAVA.
library_name: CDC_B11221