title: DNA-Seq of D. melanogaster Gateway Entry Clones: Sample GDEf10001-GDEf10121
design: "Briefly, we use a barcoded M13 forward primer (solexa tag + barcode = M13 F) and M13 reverse primer to amplify ORF clones. The PCR products are then tagmented resulting in DNA fragments starting with solexa tag + barcode + M13 + verctor and ending with anywhere in the ORFs or vector. After a size selection, a paired-end Solexa sequencing is performed on the tagmentation library. One end is solely for barcode reading, and the other end (3’ end) for ORF identification."
library_name: kiloseq_1: GEO09517