title: GSM2859227: K002000059_48026; Mus musculus; RNA-Seq
library_construction_protocol: All animals were perfused with phosphate buffered saline and kidneys were extracted immediately afterwards. Then they were divided into to halves and immediately put into liquid nitrogen. Afterwards they were stored at -80°C. until RNA preparation. From 1 µl input of total RNA, ribosomal RNA was removed using biotinylated target-specific oligos combined with Ribo-Zero rRNA removal beads. Samples of cytoplasmic rRNA wer depleted by the Ribo-Zero Human/Mouse/Rat kit. RNA was fragmented into small pieces using divalent cations under elevated temperature. After copying the cleaved RNA fragments into first strand cDNA using random primers and reverse transcriptase, the second strand cDNA synthesis was performed with DNA DNA Polymerase I and RNase H. Then single'A' base was added and subsequently adapter ligation took place. RNA libraries were prepared for sequencing using standard Illumina protocols RNA-seq total RNA – rRNA depleted by the Ribo-Zero Human/Mouse/Rat kit.
attributes.tag: GEO Accession
attributes.value: GSM2859227