title: GSM4509253: con-2; Sus scrofa; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Mini Best Universal RNA Extraction kit (Takara, Dalian, China). rRNAs were depleted with RNA sample purification beads, which employ an RNaseH-based method. Then, the RNA was fragmented and reverse transcribed. The cDNA library was subjected to end repair, poly(A)-tailing, adaptor ligation, and PCR amplification of 12–15 cycles for sequencing library construction.
attributes.tag: GEO Accession
attributes.value: GSM4509253