title: Iso-Seq of potato cv. Rywal: mock inoculated leaf
design: Plant tissue was homogenized and total RNA was isolated using RNAeasy Plant Mini kit (QIAGEN). Residual DNA was digested using Dnase I (Invitrogen).  PacBio SMRT sequencing library was prepared following Iso-Seq protocol (PacBio). For size selection, the full-length cDNA was fractioned into three contiguous size ranges (12kb, 23kb, and >3kb) on SAGE ELF before constructing SMRTbell templates.
library_name: S1_1-2kb_cellA