title: GSM3712154: CX3CR1hiLy6Cint subset in situ (single-cell RNA-Seq); Mus musculus; RNA-Seq
library_construction_protocol: Inflammatory monocyte cells were sorted and targeted single-cell RNA-seq analysis was conducted using BD Rhapsody Single-Cell Analysis System (BD BioScience) following the manufacture's protocol. In short, the single cell suspension was loaded into a BD Rhapsody cartridge with >200,000 microwells and single cell capture was achieved by random distribution and gravity precipitation. The bead library was loaded into the microwell cartridge to saturation so that a bead was paired with a cell in a microwell. The cells were lysed in the microwell cartridge to hybridize mRNA molecules onto barcoded capture oligos on the beads. Then, these beads were retrieved from the microwell cartridge into a single tube for the subsequent steps of cDNA synthesis, ExoI digestion and multiplex-PCR based library construction. For the library construction, we used the custumized BD Rhapsody Immune Response Panel for mouse (BD BioScience) consisting of primer sets for 404 genes, which contains the following seven genes: Acp5, Atp6v0d2 (NM_175406.3), Atp6v0d2 (NM_175406.3), Ctsk, Foxm1, Itgb3, Nfatc1.
attributes.tag: GEO Accession
attributes.value: GSM3712154