title: GSM4513665: Knockout 2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were perfused with HBSS containing 2.5 mg/ml iron oxide and 0.1% BSA. At the end of perfusion, kidneys were removed, decapsulated, minced into 1-mm3 pieces, and digested in HBSS containing 1 mg/ml collagenase A and 100 U/ml deoxy- ribonuclease I. Digested tissue was then passed through a 100-mm cell strainer and collected by centrifugation. The pel- let was resuspended in 2 ml of HBSS, and glomeruli were collected using a magnet. The purity of glomerular was veri- fied under microscopy. Total RNA was isolated from kidney glomeruli of mice using the RNAeasy kit (Qiagen) RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4513665