title: GSM2935441: SCNT_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Six IVF or SCNT embryos of the early blastocyst stage (96 hours after fertilization or activation) were directly lysed and used for cDNA synthesis using the SMARTer Ultra Low Input RNA cDNA preparation kit (Clontech, 634936). After amplification, the cDNA samples were fragmented using a Covaris M220 sonicator (Covaris). Sequencing libraries were made with the fragmented cDNA using NEBNext Ultra DNA Library Prep Kit for Illumina according to manufacturer's instructions (New England Biolabs, E7370).
attributes.tag: GEO Accession
attributes.value: GSM2935441