title: Illumina HiSeq 2500 sequencing; Transcriptome profiling of liver samples of C/EBPß ∆uORF mice
design: Transcriptome profiling of liver samples of C/EBPß ∆uORF mice
library_name: oWT6_s
library_construction_protocol: Liver tissue from young (5 months) and old (20 months) wt and C/EBPβΔuORF mice (from six individuals per group) was homogenized on ice with a motor driven pellet pestle (Kontes) in the presence of QIAzol reagent (Qiagen) and total RNA was isolated as described in Zidek et al., 2015. RNA was isolated from tissue samples using the RNeasy® Lipid Tissue Mini Kit (QIAGEN). After incubation for 30 min at 37°C with RQ1 RNase‐Free DNase (Promega), the RNA was further purified with the RNeasy® Plus Mini Kit (QIAGEN) according to the manufacture's protocol starting at step 4. Preparation of the sequencing libraries was performed using the TruSeq Sample Preparation V2 Kit (Illumina) according to the manufacturer's instructions.
attributes.tag: Experimental Factor: genotype
attributes.value: wild type genotype
attributes.tag: Experimental Factor: age
attributes.value: 19