title: GSM3106758: Exp #1-WT-3hr KCl; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the miRNeasy Mini Kit (Qiagen). RNA sequencing (RNA-Seq) libraries were prepared using the KAPA Stranded RNASeq Kit with RiboErase (Kapabiosystems) in accordance with the manufacturer's instructions. Briefly, 500ng of total RNA were used for ribosomal depletion and fragmentation. Depleted RNA underwent first and second strand cDNA synthesis. cDNA was then adenylated, ligated to Illumina sequencing adapters, and amplified by PCR (using 9 cycles). Final libraries were evaluated using fluorescent-based assays including PicoGreen (Life Technologies) or Qubit Fluorometer (Invitrogen) and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent2100).
attributes.tag: GEO Accession
attributes.value: GSM3106758