title: sequences of bacteriamice colon
design: we collected faeces from children with normal VA (VAN) and VA deficiency (VAD)  for transplantation into  germ-free (GF) mice. Extracted microbial DNA from colon samples. The V3 and V4 regions of the bacterial 16S ribosomal RNA (rRNA) gene were amplified with primers 338F and 806R using an ABI GeneAmp 9700 PCR thermocycler. Purified amplicons were pooled in equimolar concentrations and paired-end sequenced (2  309) on an Illumina MiSeq platform .
library_name: VAD-FMT4