title: GSM4514180: DU9; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from cells using the Qiagen RNA extraction kit. Libraries were prepared using the INCPM-mRNA-seq. Briefly, the polyA fraction (mRNA) was purified from 500 ng of total input RNA followed by fragmentation and the generation of double-stranded cDNA. After Agencourt Ampure XP beads cleanup (Beckman Coulter), end repair, A base addition, adapter ligation and PCR amplification steps were performed. Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent). Sequencing was done on a Nextseq 75 cycles high output kit, allocating 20M reads per sample (Illumina; single read sequencing).
attributes.tag: GEO Accession
attributes.value: GSM4514180