title: NextSeq 500 sequencing; Comparison of fibroblasts from ARH3-mutated patients and controls by transcription profiling.
design: Comparison of fibroblasts from ARH3-mutated patients and controls by transcription profiling.
library_name: Q334_A2_s
library_construction_protocol: Samples were obtained from fibroblasts cell cultures originating from patient's skin. Total RNA was isolated from 5x10^5 harvested cells by RNeasy Micro Kit (Qiagen), according to the procedure for animal cells. Poly(A) RNA Selection Kit (Lexogen) was used for mRNA extraction. SENSE Total RNA-Seq Library Prep Kit (Lexogen).
attributes.tag: Experimental Factor: genotype
attributes.value: ARH3 mutation