title: Illumina HiSeq 2500 sequencing; RNAseq on touch-treated Arabidopsis thaliana plants
design: RNAseq on touch-treated Arabidopsis thaliana plants
library_name: Touch 180 min rep 2_s
library_construction_protocol: Samples were quickly collected from the petri dishes with fine tweezers, dried with tissue paper, placed into an eppendorf tube and immediately snap frozen. Plants were grown in vitro for two weeks on petri dishes containing MS media with 2% sucrose. Plants were grown under long day conditions with approx. 100 micromol photons per second per square meter Plants were sprayed with water using a standard hairspray bottle (mister) RNA was prepared using the Sigma Plant Spectrum Total kit, with on-column DNA digestion and Ambion DNA-free kit Libraries were constructed using the Illumina RiboZero Plant kit
attributes.tag: Experimental Factor: stimulus
attributes.value: mechanical stimulation
attributes.tag: Experimental Factor: time
attributes.value: 180