title: Iso-seq transcriptome Argulus siamensis male
design: The total RNA from Argulus siamensis tissues were isolated using Trizol reagent and Pacific Biosciences Iso-Seq sequencing protocol was followed to generate sequencing libraries. The Clontech SMARTER cDNA synthesis kit with Oligo-dT primers was used to generate first and second-strand cDNA from polyA mRNA. After a round of PCR amplification, the amplified cDNA was subjected to size fractionation and then SMRTbell hairpin adapters were ligated onto the templates. The fractions with average size of 8 kb was sequenced on a SMRT cell using the Sequel Chemistry V2.1 on the  Pacific Biosciences Sequel platform at AgriGenome Labs Pvt Ltd, Kochi, India.
library_name: Argulus male Run-2