title: N_Ex_dsrB
design: DNA samples were subject to 454 pyrotag sequencing as in (S E Dowd et al. 2008).  Pyrotag sequencing employed dsrB primers DSR2060F 5’-CAACATCGTYCATACMCAGGG (Oakley et al. 2011) and DSR4R 5’-GTGTAGCAGTTACCGCA (M Wagner, A J Roger, et al. 1998).   All samples were sequenced by a Roche 454 FLX instrument with titanium reagents following manufactures protocols (Roche Inc.). Initial generation of sequence library consisted of a one-step 25 cycle PCR with Hot Start plus Taq polymerase (Qiagen, Inc., Valencia, CA).
library_name: Needles_Ex_dsrB