title: GSM3502447: ESC_WT_G4_L13065-1; Mus musculus; OTHER
library_construction_protocol: Tissues were homogenized in Collagenase solution, fixes with 2% FA at room temperature, 10 min and the cells were lysed in custom-made lysis buffer (10mM Tris pH7.5, 10mM NaCl, 5mM MgCl2, 0.1mM EGTA, Protease Inhibitor). Upon PBS wash, samples were snap-frozen and stored at -80°C. Samples were then digested with DpnII, re-ligated, de-crosslinked and purified. The 3C-library was then sheared using a Covaris sonicator (duty cycle: 10%, intensity: 5, cycles per burst: 200, time: 2 cycles of 60 s each, set mode: frequency sweeping, temperature: 4 to 7 °C). Adaptors were added to the sheared DNA and amplified according to Agilent instructions for Illumina sequencing. The library was hybridized to the custom-designed SureSelect beads and indexed for sequencing (50 to 100 bp paired-end) following Agilent instructions.
attributes.tag: GEO Accession
attributes.value: GSM3502447