title: GSM3035112: F-AGDP1 ChIP rep2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: 1 g of 10-day-old proAGDP1::3xFlag-gAGDP1 transgenic plants and wild-type control were cross-linked with 1% formaldehyde for 20 min and ground into fine powder in liquid nitrogen. Chromatin was isolated and sheared into 200 to 500 bp fragments by sonication. The sonicated chromatin was incubated with 2 µg of anti-Flag antibody and 25 ul Dynabeads Protein G for 5 hours. The precipitated chromatin DNA was then recovered and purified with a standard phenol–chloroform method. ChIP samples were sent to the Genomics Core Facilities of the PSC (Shanghai Center for Plant Stress Biology, Shanghai, China) for library construction according to the manufacturer's (Illumina) instructions and libraries were sequenced on an Illumina Hiseq 2500 platform.
attributes.tag: GEO Accession
attributes.value: GSM3035112