title: GSM4509034: 293FT cells, RfxCas13d circFAM120A gRNA1 replicate2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs from NT or circFAM120A KD cells were extracted with Trizol (Life Technologies) according to the manufacturer's protocol. For gene expression analysis after circFAM120A KD (poly(A)+ RNA-seq), polyadenylated (poly(A)+) RNA preparation was carried out as following. Briefly, total RNAs (8 μg) were incubated with oligo(dT) magnetic beads to isolate poly(A)+ RNAs, which were bound to beads, selection was performed three times to ensure the purity of poly(A)+ RNA populations. Poly(A)+ RNA-seq libraries were prepared using Illumina TruSeq Stranded Total RNA LT Sample Prep Kit. All libraries were size-selected with AmpureXP beads (Agencourt) and quantified using Agilent Bioanalyzer 2100 and QuBit high-sensitivity dsDNA assay. Size-selected libraries were subjected to deep sequencing with Illumina NextSeq 500 at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China.
attributes.tag: GEO Accession
attributes.value: GSM4509034