title: GSM2693623: CD4-ICOSpos-B447; Mus musculus; RNA-Seq
library_construction_protocol: Activated tumor infiltrating ICOS+ CD4 T cells were sorted directly into RLT buffer (volume) using a FACS AriaFusion cell sorter (BD).  Lysates were homogenized using a QiaShredder and RNA extracted using RNeasy Micro kit (Qiagen) according to the manufacturer’s protocol. Due to low RNA yield from sorted T cell populations from individual mice, libraries were prepared using Ovation single-cell RNAseq system (NuGEN).  Indexed libraries were analyzed by 76-nucleotide paired end sequencing on a HiSeq 4000 system (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2693623