title: GSM3444801: atac_spleen_RFPpGFPp_Foxp3; Mus musculus; ATAC-seq
library_construction_protocol: CD4 T cells from asthma mice were sorted based on RFP and GFP signals as indicated. Th1 cells from toxo infected mice were sorted as CD4+ CD44+ CD62L- population. Tmem cells from control mice were sorted as CD4+ CD44hi CD62L- population. ATAC-seq was performed according to published protocol (Buenrostro et al., 2013) with  minor modification. 50,000 cells were pelleted and washed with 50ul 1xPBS, followed  by treatment with 50ul lysis buffer (10mM Tris-HCl, pH7.4, 10mM NaCl, 3mM MgCl2,  0.1% of IGEPAL CA-630). After pelleting the nuclei by centrifuging at 500x g for  10min, the pellets were re-suspended in 40ul transposition reaction with 2ul Tn5  transposase (Illumina Cat# FC-121-1030) to tag and fragmentalize accessible chromatin.  The reaction was incubated at 37ºC with shaking at 300rpm for 30min. The  fragmentalized DNAs were then purified using Qiagen MinElute Kit and amplified with  10-11 cycles of PCR based on the amplification curve. Once the libraries are purified  using Qiagen PCR Cleanup Kit, they were further sequenced for 50 cycles (paired-end  reads) on HiSeq2500.
attributes.tag: GEO Accession
attributes.value: GSM3444801