title: RNA-seq of NPC1 c.3591+2C>T biological replicate 1
design: Cells were harvested, washed three times with 1X PBS, pelleted, then resuspended in TRIzol Reagent (Thermo Fisher Scientific). Isolation of mRNA was isolated following the manufacturers protocol. RNA sequencing was performed by the Centre for Applied Genetics in Toronto using the Illumina HiSeq 2500 system, producing 120-bp paired-endreads
library_name: LIB007