title: 16S sequences of human feces: healthy control
design: fresh stool samples were collected from the study participants and immediately stored at -80C until the DNA extraction. DNA was extracted with the MagNA Pure LC DNA Isolation Kit III (Bacteria, Fungi) (Roche, Mannheim, Germany) according to the manufacturers instructions. Hypervariable region V1-V2 was amplified (primers: 27F-AGAGTTTGATCCTGGCTCAG; R357-CTGCTGCCTYCCGTA) and sequenced using Illumina Miseq technology (Illumina, Eindhoven, The Netherlands)
library_name: Control19_inhouserelative