title: GSM4453719: HY19; Mus musculus; RNA-Seq
library_construction_protocol: Brains were removed and flash frozen on dry ice; brain regions were later punched out, placed in Trizol and homogeneized; Total RNA was isolated from the aqueous layer obtained post chloroform extraction, using the Direct-zol RNA Miniprep Plus kit (Zymo, catalogue # R2071) according to manufacturer's instructions, with the additional DNase I in-column treatment step. RNA libraries were prepared for sequencing using NEBNext® UltraTM RNA Library Prep Kit, following standard protocols
attributes.tag: GEO Accession
attributes.value: GSM4453719