title: The single-cell RNA sequencing of the tumor tissue from the OSCC patients #1
design: Single-cell suspensions were loaded onto the Chromium Controller in proportion to their respective cell counts, generating the single-cell GEMs (gel beads in emulsion) per sample. Each sample was loaded into a separate channel. Libraries were then prepared with Chromium Single Cell 3 v3 Library and Gel Bead Kit (10x Genomics). The cDNA content of each sample after cDNA amplification of 15 cycles was quantified and quality checked using a High-Sensitivity DNA chip with a 2100 Bioanalyzer (Agilent Technologies) to determine PCR amplification cycle counts. After that, samples were diluted to 3.5nM for loading onto the NovaSeq 6000 (Illumina).
library_name: hs_S1