title: GSM1977398: R; Ovis aries; RNA-Seq
library_construction_protocol: All visible follicles (>2.5mm in diameter) from the ovaries were dissected free from the ovarian stroma into 1 ml D-PBS. The granulosa cell layer was then gently scraped into D-PBS. RNA was extracted from granulosa cells using the RNeasy Micro Kit (Qiagen, Germany) according to the manufacturer's protocol. RNA-seq transcriptome libraries were prepared following the TruSeq RNA sample preparation Kit (Illumina, San Diego, CA). 5 microgram of RNA from each group was used to synthesize the cDNA.The cDNA was then eluted with EB buffer for end repair and poly (A) addition. Finally, sequencing adapters were ligated to the fragments, followed by PCR amplification (15 cycles) using Veriti 96 Well Fast Thermal Cycler (Applied Biosystems) to create cDNA libraries.
attributes.tag: GEO Accession
attributes.value: GSM1977398