title: GSM4018725: Mouse_Nuclei_Grik1-_6; Mus musculus; RNA-Seq
library_construction_protocol: The tissue was dissociated fresh or the tissue was frozen. Cells or nuclei were extracted, fixed, and labeled with appropriate SABER FISH probes. Specific cellular or nuclear populations were FACS isolated and the RNA was extracted from the cells or nuclei using the RecoverAll Kit. For live cells, Grik1-GFP plasmid was electroporated at P2 into the developing mouse retina. The retina was harvested at P40, and the cells were dissociated. GFP+ cells were FACS isolated into Trizol. The RNA was extracted based upon Trizol manual. cDNA was generated using the SMART-Seq v.4 kit. The indices were added using the Nextera XT Kit.