title: Northern sudanian soil 06
design: The 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines.
library_name: Sen25