title: GSM3639323: 2a-HET1_H3K4me3; Mus musculus; ChIP-Seq
library_construction_protocol: Mouse amygdala tissue was dissected from adult males. Nuclei were isolated using Nuclei EZ prep Kit (Sigma, NUC-101), and counted. 20,000 nuclei were used as input. ChIP-Seq library preparation was performed as described in PMID 25607992 with two exceptions. 1) We used a kit to generate sequencing library in one-tube reactions (NEB, E7103S). 2) We spiked in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher, SKU: 19-1001). We used the rabbit monoclonal H3K4me3 antibody (Thermo, clone #RM340).
attributes.tag: GEO Accession
attributes.value: GSM3639323