title: GSM1154269: white collar 2 knockout mutant; Neurospora crassa; RNA-Seq
library_construction_protocol: total RNAs of each sample were extracted and small RNAs were enriched with 5% polyethylene glycol (MW8000) and 500 mM NaCl illumina standard TruSeq small RNA preparation. For each sample, small RNAs of 18-30 nt were purified from PAGE gel. The 3' and 5' RNA adaptors were then ligated and RT-PCR were performed to amplify the library (18 cycles). Libraries were analysed with Illumina HiSeq 2000. Please see instruction of illumina Truseq small RNA preparation guide and BGI website for detailed information
attributes.tag: GEO Accession
attributes.value: GSM1154269