title: Deep sequencing of rainbow trout female embryonic gonads using 454 GS FLX Titanium technology
design: The Roche 454 Titanium technology was used to sequence cDNA libraries made from gonads of all-female (XX) embryos sampled at 35 days post fertilization (dpf), i.e., at the very beginning of the molecular sex differentiation period in order to search for genes specifically expressed during gonadal differentiation
library_name: 35_dpf_Female
library_construction_protocol: Gonads were sampled in 35 days post fertilization (dpf) embryos obtained from genetically all-female (XX) rainbow trout populations. RNA extraction was carried out using the MELT Total Nucleic Acid Isolation System (Applied Biosystems/Ambion). After cDNA synthesis and amplification using SMARTer PCR cDNA Synthesis (Takara Bio Europe/Clontech), cDNA was nebulisated and libraries were prepared using the GS DNA Library Preparation Kit according to manufacturer's recommendations (Roche Applied Science, Meylan, France). Final libraries were quantified with the SlingShot kit using the Fluidigm Digital Array and sequenced using the 454/Roche GS FLX Titanium chemistry (GS Titanium LV emPCR and sequencing kits) according to manufacturer's informations. Each sample was sequenced on one region of a two regions Picotiterplate.