title: Gastric cancer cell line isolated by clonal selection of MKN45 cell line
design: For the RNA-Seq library construction, the Illumina "TruSeq mRNA stranded kit" (code RS-122-2101) was used, with 1000ng of input RNA. Initial RNA quality was assessed by means of Agilent Bioanalyzer (RNA 6000 Nano kit, Agilent code 5067-1511) and the corresponding concentration with Qubit (RNA BR Assay kit code Q10210). Library quality was then assessed by Bioanalyzer using DNA HS kit by Agilent (code 5067-4626) and libraries quantified by Qubit with DNA kit HS code Q32851. All libraries were subsequently normalized to 10nM with TrisHC and then pooled. Finally, 1.8pM of the pooled RNA was loaded into an Illumina NextSeq 500.
library_name: GTL16_WT_rep2