title: 16S rRNA gene amplicon data of an enrichment of a filtered compost soil sample (2.7 µm) in M9 medium with 25 mM acetonitrile as sole nitrogen source
design: A filtered compost soil sampe (2.7 µm) of the Experimental Botanical Garden Göttingen, Germany (51°33‘22.6“N 9°57‘16.2“E, 180 m) was used as inoculum for M9 medium (Atlas, 2010) with 25 mM acetonitrile instead of NH4Cl as sole nitrogen source. The culture was incubated at 25°C for four days. Genomic DNA was extracted with the MasterPure complete DNA and RNA kit (Epicentre). The 16S rRNA gene V3-V4 region was amplified using the primers S-D-Bacter-0341-b-S17 and S-D-Bact-0785-a-A-21 (Klindworth et al., 2013). The sequencing library was generated with the Nextera XT DNA library prep kit (Illumina). For sequencing, the MiSeq reagent kit version 3 (Illumina) was used.
library_name: REGE_a_86