title: GSM3501234: WT 1; Mus musculus; RNA-Seq
library_construction_protocol: SMGs were removed, and RNA was harvested using an Ambion RNA isolation Kit. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared using Illumina's TruSeq stranded protocol. Briefly, polyA mRNA was selected using beads coated with polyT oligonucleotides. Purified polyA mRNA was subsequently fragmented, and first and second strand cDNA synthesis performed using standard Illumina mRNA TruSeq library preparation protocols. Double stranded cDNA was subsequently processed for TruSeq dual-index Illumina library generation.
attributes.tag: GEO Accession
attributes.value: GSM3501234