title: GSM2593658: Normal water_5; Mus musculus; RNA-Seq
library_construction_protocol: Intestines were removed, flash frozen on dry ice, and total RNA was extracted from colon of mice using TRIZOL Reagent (Life Technologies). 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs), and converted to Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2593658