title: GSM1155383: DGM-11016_LA-BC; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from harvested nonsmoker and smoker basal cells was extracted with TRIzol with subsequent RNA clean-up using the RNeasy MinElute RNA purification kit to remove residual DNA. The TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) was utilized as per protocol to generate cDNA that was then purified with QIAquick PCR purification kit (Qiagen, Valencia, CA). Samples were loaded onto an Illumina flowcell for paired-end 101 x 2 sequencing reactions using the Illumina HiSeq 2000, using a single flow cell with 4 samples multiplexed per lane of flow cell.
attributes.tag: GEO Accession
attributes.value: GSM1155383