title: Illumina MiSeq sequencing; qiita_ptid_3952:11402.MC008
design: A cross-sectional observational study of the gut microbiome in Peruvian infants at 6 or 12mo of age
library_name: 11402.MC008
library_construction_protocol: Sequences were amplifed using the F515/R806 primer pair, both of which were modified to contain an Illumina adapter region. An 8bp Hamming error-correcting barcode included on the 3' end of the forward primer to allow for sample multiplexing.
attributes.tag: barcode
attributes.value: ACTGTCTC
attributes.tag: center_name
attributes.value: UC Davis Slupsky Lab
attributes.tag: center_project_name
attributes.value: Peruvian_Infant_Iron_Microbiome
attributes.tag: linker
attributes.value: GT
attributes.tag: linker_primer_sequence
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: pcr_cond
attributes.value: 94degC_3min; denaturation:94degC_45sec; annealing:50degC_1min; elongation: 72degC_90sec; final elongation:72degC_10min; 40total cycles
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGCCAGCMGCCGCGGTAA
attributes.tag: reverse_primer
attributes.value: GGACTACHVGGGTWTCTAAT
attributes.tag: run_center
attributes.value: UC Davis Genome Center
attributes.tag: run_date
attributes.value: 3/3/2016
attributes.tag: samp_vol_we_dna_ext
attributes.value: 237.9
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4