title: RNA-seq of 2 month old mice brain total RNA with two replicates
design: Total brain RNAs of 24-month-old C57BL/6Ęmale mice and 2-month-old C57BL/6Ęmale mice depleted of rRNA and mRNA using Ribominus kit for human/mouse (Invitrogen) with additional Locked Nucleic Acid probes containing poly-T sequence and end-labeled with biotin (EXIQON).  Poly-A-depleted RNA (80ng) was then polyadenylated with E. coliĘPoly(A) Polymerase (New England BioLabs), following the manufatureŐs instructions, and purified with 90ľl AMPure XP beads (Beckman Coulter Life Sciences) and 270ľl Isopropanol. The RNAs were eluted with 11ľl RNase-Free water, and used for cDNA library construction using Clontech SMART-Seq v4 Ultra Low Input RNA Kit, following manufactureŐs protocol.
library_name: Young_2_replicates