title: GSM3638034: sgControl rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was coprecipitated with GlycoBlue (ThermoFisher #AM9516) following manufacturers protocol; in brief, 3uL (final concentration 45ug/mL) of GlycoBlue was added to TrizolLS-separated aqueous phase followed by one volume isopropanol and incubated at -20C for 30 min before pelleting RNA by centrifugation. Isolated RNA was treated with DNase I (ThermoFisher #EN0521) which was inactivated by addition of EDTA and incubation at 65C. PolyA+ RNA libraries were generated using NEBNext PolyA mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L) and indexed using NEBNext Multiplex Oligos for Illumina (E6440L). 100bp paired-end sequencing was performed on the Illumina HiSeq 1500 with depth of 50 million reads per sample.
attributes.tag: GEO Accession
attributes.value: GSM3638034