title: Illumina HiSeq 4000 sequencing; RNA-seq in HSJD-DIPG-007 cells treated with JQ1, ICG-001, JQ1 and ICG-001 and DMSO as control.
design: RNA-seq in HSJD-DIPG-007 cells treated with JQ1, ICG-001, JQ1 and ICG-001 and DMSO as control.
library_name: HSJD-DIPG-007_JQ1_2_s
library_construction_protocol: HSJD-DIPG-007 cells (CVCL_VU70) were treated for 48 hours after treatment before harvesting. 250nM JQ1 and 2.5uM ICG-001 in DMSO was added for 48 hours. RNA was extracted using TRIzol reagent (Life Technologies) and digested with DNaseI (Thermo Scientific) according to manufacturer's instructions. Libraries from RNA were synthesized using the TruSeq RNA Library Prep Kit v2 (Illumina) as per manual instructions.
attributes.tag: Experimental Factor: stimulus
attributes.value: JQ1 (250 micromolar)