title: GSM4294263: SNU16-Input-(control for H3K4me1,H3K27ac); Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-Seq, cells were cross-linked with 1% formaldehyde for 10 min at room temperature, and reactions were stopped by adding glycine to a final concentration of 0.2 M. Chromatin was extracted and sonicated to 500 bp. EBF1 (H00001879-M01, Abnova), H3K27me3 (#07-449, Millipore), h3k27AC (ab4729, Abcam), or H3K4me1 (ab8895, Abcam) antibodies were used for chromatin immunoprecipitation (ChIP). Input DNA from cells prior to immunoprecipitation were used to normalize ChIP-seq peak calling. ChIPed DNA (10-30 ng) was used for ChIP with DNA sequencing (ChIP-seq) library construction following manufacturer protocols (New England Biolabs). For RNA-Seq, sequencing libraries were constructed from total RNA of GC cell lines with 300-400 bp insert lengths ChIP-seq, RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM4294263