title: GSM4367763: CT24WT2; Oryza sativa; RNA-Seq
library_construction_protocol: Total RNA was extracted from up-ground rice seedlings using Trizol (Invitrogen, Carlsbad, CA, USA) following manufacturer's guidelines. For each treatment, RNA extraction was performed in three biological repeats (5 plants per repeat). NanoPhotometer® (IMPLEN, CA, USA) and Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA) were used for purity and quantitative assessment of RNA samples, respectively. In addition, the integrity of RNA was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according the manufacturer's instructions and index codes were added to attribute sequences to each sample.
attributes.tag: GEO Accession
attributes.value: GSM4367763