title: GSM3033446: KNO3_3hr_3; Dunaliella viridis; RNA-Seq
library_construction_protocol: Total RNA was extracted from the 30 ml frozen pellets of the 39 samples using the RNeasy Plant Mini Kit (Qiagen) following standard procedures for use with a QIAcube.  DNA was removed from total RNA using RNase-Free DNase (Qiagen), and rRNA was depleted from the samples using the Ribo-Zero™ Magnetic Kit (Plant Leaf) (Epicentre Cat. No. MRZPL1224).  After each processing step, RNA quality was confirmed using a Bioanalyzer RNA Pico Chip (Agilent) Strand specific cDNA libraries with 300–450 bp inserts were generated with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England BioLabs).  Sample libraries were barcoded using the TruSeq DNA LT Sample Prep Kit, Indexed Adapter Sequences (Illumina).  Final quality of the libraries was confirmed using a Bioanalyzer DNA high sensitivity chip (Agilent).  Libraries were split into 2 groups and submitted for single end 125 bp Illumina HiSeq 2500 sequencing runs in 2 flow cells at the North Carolina State University Genome Sciences Laboratory (GSL).
attributes.tag: GEO Accession
attributes.value: GSM3033446