title: 16S rRNA Sequences from freshwater Rocky Mountain lakes
design: We extracted genomic DNA with the PowerWater DNA Isolation Kit (MoBio Inc., Carlsbad, CA, USA) and used PCR to amplify the V4-V5 region of bacterial 16S rRNA genes using the 515 forward and 806 reverse primer set. Demultiplexing, quality filtering, denoising, merging of paired-end reads, singleton and chimera removal, and taxonomic assignment of the raw 16S rRNA gene sequences were completed using the DADA2 pipeline. Sequences with no more than 1 bp difference were considered unique amplicon sequence variants (ASV). During the quality filtering step, we set a minimum length of 50 bps, trimmed the left ends of both forward and reverse reads at 15 bps and the right end of forward reads at 100 bps and reverse reads at 110 bps. The max error rate was set at 1 and the minimum quality score was set to 13 at the denoising step.
library_name: KV008