title: GSM4294198: CTCF_ChIPseq_KO; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIPseq, cells were fixed with 1% formaldehyde and insoluble chromatin fraction were isolated. Chromatin were sonicated to 200 to 500 base pair and protein-DNA complexes were pulled down by antbodies.For RNAseq, cells were lysed with Trizol, RNAs were isolated using phenol-chloroform extraction. For HiC cells were fixed with 2% formaldehyde and then insoluble chromatin fraction were isolated following Arima HiC kit protocol. For ChIPseq samples, libraries were prepared using ThruPLEX DNA-seq kit (R400428) according to manufacturer's instruction. Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing. For RNAseq samples,  libraries were prepared using SMART Seq v4 Ultra Low Input RNA kit from Takara and Nextera XT DNA library prep kit from Illumina according to the manufacturer's instructions. For HiC samples, libraries were prepared using Arima HiC kit and Swift biosciences® Accel NGS® 2S plus DNA library kit following the low input procedure instructions.
attributes.tag: GEO Accession
attributes.value: GSM4294198