title: GSM4293665: H3K9me3_WT_6m_M_rep3; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP samples were purified using Agencourt AMPure XP beads (Beckman Coulter) and quantified with Qubit (Invitrogen). ChIP-seq libraries were prepared from 2 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode), according to manufacturer's instructions. Illumina compatible indexes were added through PCR amplification (7 cycles). Amplified libraries were purified and size-selected using Agencourt® AMPure® XP beads (Beckman Coulter) to remove unincorporated primers and other reagents. Prior to analyses DNA libraries were checked for quality and quantified using a 2100 Bioanalyzer (Agilent). Libraries were sequenced on Illumina Hiseq 4000 sequencer as Paired-End 50 base reads following Illumina's instructions. Image analysis and base calling were performed using RTA 2.7.3 and bcl2fastq 2.17.1.14.
attributes.tag: GEO Accession
attributes.value: GSM4293665