title: PT05_TP_300
design: ScRNA-seq was performed according to the manufacturers instructions of Smart-seq 2. Reverse transcription was performed using Superscript reverse transcriptase (Takara) and locked TSO oligonucleotides, (Exiqon). Full-length cDNA preamplification was conducted with 22 cycles of quantitative polymerase chain reaction (PCR) amplification and HiFi-HotStart ReadyMix (KAPA Biosystems). Subsequently, Ampure XP beads (Beckman) were used for the purification. An Agilent high-sensitivity DNA chip was used to ensure the size and distribution of the cDNA library. Barcoded libraries were fragmented and tagged using a Nextera XT DNA preparation kit (Illumina). Then, we used reagents from the Nextera XT kit to amplify adapter-ligated fragments. Pooled libraries with unique N5-N7 barcodes were sequenced using a HiSeq 2500 instrument (Illumina) and single-end 50-bp read flow cells.
library_name: Sample 300