title: GSM4293751: WTA replicate 3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was isolated from N2 and hira-1(gk835598) using the Tissue Ruptor (Qiagen) and TRIzol Reagent (Invitrogen) followed by isopropanol precipitation. Then RNA was further purified using RNeasy Mini Kit (Qiagen). mRNA was purified by polyA-tail enrichment, fragmented, and reverse transcribed into cDNA (Illumina TruSeq). cDNA samples were then end-repaired, adaptor-ligated using the SPRI-works Fragment Library System I (Beckman Coulter Genomics) and indexed during amplification. Libraries were quantified using the Fragment Analyzer (Advanced Analytical) and qPCR before being loaded for paired-end sequencing using the Illumina NextSeq sequencer.
attributes.tag: GEO Accession
attributes.value: GSM4293751