title: GSM4142822: 24hpi_3_mRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol, followed by DNase I digestion (Epicentre) for 15 min at 37°C. An rRNA-depleted, RNaseR treated RNA library, a small RNA library, and a mRNA library were constructed for the identification of circRNA, miRNA, and mRNA, respectively. To prepare for circRNA sequencing, total RNA was treated by RNase R (RNR07250, Epicentre) for 20 min at 37°C, followed by rRNA depletion using a Ribo-Zero Gold Kit (Epicentre) under the manufacturer's recommendations. After purification, the RNA product was fragmented using VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (Vazyme), along with total RNAs used for both miRNA and mRNA sequencing separately.
attributes.tag: GEO Accession
attributes.value: GSM4142822