title: Illumina HiSeq 2000 sequencing; Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability
design: Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability
library_name: wee1-1/rnh2a - 0mM HU - 3
library_construction_protocol: The WEE1 knockout (wee1-1) and the RNH2A/WEE1 double knockout (rnh2a/wee1-1) seeds were germinated on control medium on a nylon mesh. Transferred 5 d after germination to control medium or medium supplemented with 2 mM HU 24h after the transfer 200 root tips (<2 to 3 mm) were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with the RNeasy plant kit (Qiagen). Illumina True-Seq libraries were generated from cDNA according to the manufacture’s protocol and sequenced on a HiSeq2000.
attributes.tag: Experimental Factor: genotype
attributes.value: wee1-1/rnh2a
attributes.tag: Experimental Factor: compound
attributes.value: none