title: V4 region of eukaryotic rRNA gene
design: 300bp paired-end sequencing of  the library was performed with the Illumina MiSeq 2500 platform with NEXTflex  DNA sequencing kits and an identifying NEXTflex DNA barcode with 8-base  indices .Three different PCR from the same cDNA: alpha, beta and gamma. Run  1.
library_name: S12_S23_S32