title: GSM4237816: Ctrl 2; Mus musculus; RNA-Seq
library_construction_protocol: MLNs (2 MLNs pooled/sample, n=2) were removed from WT C57BL/6J mice and were crushed through a 40 micron strainer. CD11c+ cells were sorted from MLN samples using a CD11c magnetically activated cell sorting (MACS) kit (Miltenyi Biotec cat#: 130-108-338). CD11c+ cells from each sample (~9x104 cells) were then split into two wells and resuspended in RPMI (Sigma) with 10%FBS and cultured in with either control (H2O) or with Ffar2 agonist (10μM) at 37C for 6hr. After incubation, the cells were resuspended in RLT buffer and RNA was immediately isolated using an RNeasy Micro kit (Qiagen cat#: 74004). RNA quality verification, library preparation, and sequencing were performed at BPF Next-Gen Sequencing Core Facility at Harvard Medical School. RNA samples showed an average 9.0 of RNA integrity number (RIN) and libraries were generated using SMART-seq v4 Ultra Low Input RNA kit (Takara Bio Inc.).
attributes.tag: GEO Accession
attributes.value: GSM4237816