title: WGS of S. cerevisiae: delta ade13 mutant_mix_6
design: For each of the five genes, we select 30 largely independent clones which the focal essential gene has been deleted and their corresponding parental strain in the yTHC stock. So we sequenced the genomes of the 30 * 5 + 5= 155 clones. Three genomes were pooled together and we had 55(10×5+5) samples for the five essential genes. Fragments between 400bp and 600bp were collected for library construction and samples were sequenced on an Illumina Hi-seq 2000 platform. Approximately 8 million 125bp reads were generated for each sample, corresponding to an average sequencing depth of ~80. The gene_sample_0 represent the parental strain from yTHC stock.