title: mixed methanotrophic culture with high Cu concentration
design: Samples of 50 mL were collected at the exponential phase of batch reactors fed with bio-CH4, grown at EC-NH4 medium and supplied with copper. Genomic DNA was isolated and purified using the PowerSoil DNA Isolation Kit (QIAGEN Bioinformatics, Germany) [20]. The V4 region of microbial 16S rRNA genes was amplified using 515F/806R primers. Sequencing was conducted using the MiSeq platform (Illumina) and NanoDrop (Thermo Scientific) assessed samples purity and the Qubit fluorimeter (Life Technologies) was used for samples quantification.
library_name: high-Cu