title: GSM2857708: E87-Batf3-8 rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from T cells using RNeasy kits (Qiagen, Valencia, CA) according to the manufacturer's protocol Library construction was performed using the Illumina TruSeq Stranded mRNA library preparation kit. Briefly, Total RNA samples were assessed for concentration using the Nanodrop 8000 (Thermo Scientific) and quality using the 2100 Bioanalyzer (Agilent). One microgram of total RNA per sample was used for poly-A mRNA selection using streptavidin-coated magnetic beads. cDNA was synthesized from enriched and fragmented RNA using reverse transcriptase (Super-Script II, Invitrogen) and random primers. The cDNA was further converted into double-stranded DNA, and the resulting dsDNA was enriched with PCR for library preparation. The PCR-amplified library was purified using Agencourt AMPure XP beads (Beckman Coulter). The concentration of the amplified library was measured with a NanoDrop spectrophotometer and an aliquot of the library is resolved on an Agilent 2100 Bioanalyzer. Sample libraries are multiplexed and sequenced on a NextSeq 500 platform (Illumina) using 75bp single-end sequencing. On average, about 20 million reads were generated from each sample.
attributes.tag: GEO Accession
attributes.value: GSM2857708