title: Illumina HiSeq 2500 paired end sequencing; ChIP-Seq of BCL11A and SOX2 in lung squamous cell carcinoma (LUSC) LK-2 cell line with or without BCL11A knockdown
design: ChIP-Seq of BCL11A and SOX2 in lung squamous cell carcinoma (LUSC) LK-2 cell line with or without BCL11A knockdown
library_name: LK2_BCL11A-ContSh_Input_p
library_construction_protocol: LK-2 cells were cultured in RPMI (gibco) supplemented with 10% fetal bovine serum and 1% Penicillin/Streptomycin.    ChIP-Seq was done exactly as described in (Schmidt, D., et al. ChIP-seq: Using high-throughput sequencing to discover protein–DNA interactions. Methods 48, 240-248 (2009)). Briefly 2 x 15cm plates per cell line were formaldehyde crosslinked, nuclear fraction was isolated and chromatin sonicated using Bioruptor Pico (Diagenode). Libraries were produced at the Wellcome Trust Sanger Institute sequencing facility. Each library was split into two and run on separate lanes
attributes.tag: Experimental Factor: RNA interference
attributes.value: scrambled shRNA
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: input DNA