title: Non-NC cells dissociated from post-otic region, replicate 3
design: Sorted cells were pelleted in HBSS and immediately lysed. RNA was extracted with RNAqueous Micro Total RNA Isolation Kit, followed by removal of contaminant genomic DNA with DNase treatment for 40 min (Ambion, cat# AM1931). RNA quality was determined with the Bioanalyzer 2100 instrument (Agilent). cDNA was prepared with the Ovation RNA-Seq System V2 (NuGEN). The sequencing libraries were built using Illumina standard protocols. SR50 sequencing was performed with the HiSeq 2500 System (Illumina).
library_name: NC2-negative_3