title: gene expression of Pelteobagrus vachelli:6 hours replicate2
design: Following the manufacturers protocol of TRIzolReagent (Invitrogen, Waltham, MA, USA), the total RNA in each sample was isolated and the contaminating genomic DNA was cleared using DNase I (Takara, Dalian, PR China).[AA1]  A 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) assessed the RNA quality and a ND-2000 (Nano Drop Technologies, Wilmington, DE, USA) assessed its quantity. Shanghai Genergy Technology Co. Ltd. (Shanghai, China) performed the RNA sequencing (RNA-seq). Subsequently, the generated cDNA libraries were sequenced using the Illumina HiSeq3000 platform (Illumina, San Diego, CA, USA).
library_name: LIBQCJ305