title: OMalley_et_al2013
design: OMalley_et_al2013
library_name: D6s4B5 MEFr1
library_construction_protocol: MEFs and ES Cells were cultured in GMEM + 100Units LIF. Intermediate reprogramming samples were cultured in the presence of dox, VitC and Alk5i for 10 or 15 days. 20,000 to 200,000 cells from each sample were sorted by flow cytometry. Pelleted cells were resuspended in 500uL TRI Reagent (Invitrogen). RNA was extracted in the usual manner via phenol-chloroform extraction. RNA quality was verified using a Agilent Bioanalyzer. RNA was diluted to a concentration of 10ng/uL in 10mM TRIS ph7.6, 0.05% Tween-20 buffer. Library was constructed as published in Islam et al. Genome Research, 21, 1160-1167, doi:10.1101/gr.110882.110 (2011).
attributes.tag: Experimental Factor: individual
attributes.value: D6s4B5 MEF
attributes.tag: Experimental Factor: cell type
attributes.value: mouse embryonic fibroblast cell