title: GSM3503360: JY10769; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from thymus tissues using TRIzol reagent (ThermoFisher Scientific) and further purified with the RNeasy Plus Mini Kit (Qiagen). RNA purity was evaluated with a NanoDrop spectrophotometer (ThermoFisher Scientific) and RNA integrity was determined using a 2100 Bioanalyzer with RNA chips (Agilent Technologies). Libraries for mRNA sequencing were prepared with the TruSeq Stranded mRNA Library Prep kit (Illumina) from 250 ng of RNA. Quality analysis of libraries was performed using Agilent 4200 TapeStation with D1000 ScreenTape assays (Agilent Technologies). Libraries were pooled and then quantified by two methods: 1) on a 2100 Bioanalyzer with DNA chips using High Sensitivity DNA Kit (Agilent Technologies); and 2) with KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems).
attributes.tag: GEO Accession
attributes.value: GSM3503360