title: Targeted deep sequencing of HIV-1 314_Ae02SbNE83IX34b
design: Viral RNA was extracted from blood plasma from HIV-1 infected individual. Subgenomic area of interest, env(gp160) (HXB2 coordinates: 6225-8795) was amplified by RT-nested PCR SGA using 100ng of second round PCR product enzymatically sheared to 400bp as input. IonTorrent library was prepared according to manufacturer's instructions.
library_name: 314_Ae02SbNE83IX34b