title: GSM4369130: cdk9as_htb1kr_dmso_pol2_r1; Schizosaccharomyces pombe; ChIP-Seq
library_construction_protocol: ChIP-seq: After HCHO-crosslinking (15 min at 25°C), protein extract was prepared using Mini-bead beater (Biospec; 30 sec "ON" and 30 sec "OFF"). Lysates were precleared with Protein A agarose beads and used for IP with respective antibodies. ChIP-seq: Preparation of multiplexed ChIP-seq libraries from purified immunoprecipitated chromatin and sequencing were performed as described. In brief, the NEBNext Ultra II DNA Library Preparation kit (E7103S) was used to generate libraries using 5-10 ng of input or immunoprecipitated DNA and barcode adaptors (NEBNext Multiplex Oligos for Illumina (Set 1, E7335 and Set 2, E7500)).
attributes.tag: GEO Accession
attributes.value: GSM4369130