title: Amplicon sequencing of Active fungi: rhizosphere soil_replicate 3
design: Genomic DNA were extracted from rhizosphere soil red clover from GCEF project.. the internal transcribed spacer region of the fungal internal transcribed spacer region (ITS) was amplified using the forward primer ITS3 (5-GCATCGATGAAGAACGCAGC-3)  and the reverse primer ITS4 (5 TCCTC CGCTTATTGATATGC-3)
library_name: Af3