title: GSM4509185: N1AcSpd CD34+ rep.1; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were frozen in QIAzol and frozen in liquid nitrogen. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer's instruction. cDNA synthesis was performed using Maxima H Minus reverse transcriptase (ThermoFisher). During reverse transcription, unique barcodes were attached to each sample. cDNA of all samples was pooled and purified using Ampure XP beads (Beckman Coulter). Eluted cDNA was subjected to Exonuclease I (NEB) treatment. cDNA was PCR-amplified and tagmented using the Illumina tagmentation mix. Nextrea Index PCR primers were attached to the libraries during PCR amplification. cDNA was purified and library concentration and size were validated.
attributes.tag: GEO Accession
attributes.value: GSM4509185