title: GSM4200859: CT2; Homo sapiens; miRNA-Seq
library_construction_protocol: Approximatelly 1 mL of serum was used to isolate extracellular vesicles, using the kit Total Exosome Isolation Reagent from Serum (Invitrogen, CA, USA), according to manufacturer's instructions. MiRNAs-derived from extracellular vesicles were extracted using miRVana™ miRNA Isolation Kit (Ambion, CA, USA), modified with addition of Trizol (Invitrogen, CA, USA) in substitution to phenol. All samples were ressuspended at 20µL final volume and then mixed to form the pools. Each pool was comprised of a mix of sample as follow: (CT1 (n=5), CT2 (n=5), LA1 (n=5), LA2 (n=5), TNBC1 (n=4) and TNBC2 (n=4). All pools had were composed of individuals with matching ages. The libraries were constructed through Ion Total-RNA Seq Kit v2 (Life Technologies, CA, USA), according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4200859