title: Complete Coding Genome Sequence of Microbacterium sp. strain 10M-3C3
design: SMRT libraries were prepared using the standard PacBio protocol for 20 kb libraries (Pacific Biosciences, 2016). DNA fragments larger than 10kb were size selected by Blue Pippin (Sage Science). The average fragment size of the library was 30 kb measured by a Fragment Analyzer (Advanced Analytical Technologies, Inc). Sequencing was completed on a Pacbio RS II Single-Molecule Sequencer in one SMRT cell using P6-C4 chemistry with a 6-hour movie. Reads were assembled using Hierarchical Genome Assembly Process 3 (HGAP3) within the SMRT Analysis version 2.3.0 software from Pacific Biosciences with 23 kb seed read fragments length. Reads of inserts were filtered by quality 0.8 and read length 1kb using the PreAssembler Filter version 1 protocol. This resulted in a single circular contig 3,416,431 bp long with 407x genome coverage.
library_name: 10M-3C3