title: GSM4509043: HeLa ribo– RNA-seq replicate1; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNAs from HT29, 293FT and HeLa cells were extracted with Trizol (Life Technologies) according to the manufacturer's protocol. For circRNA expression analysis, total RNAs from HT29, 293FT and HeLa WT cells was processed with the RiboMinus kit (Human/Mouse Module, Invitrogen) to deplete ribosomal RNAs for sequencing. Ribo– RNA-seq libraries were prepared using Illumina TruSeq Stranded Total RNA LT Sample Prep Kit. All libraries were size-selected with AmpureXP beads (Agencourt) and quantified using Agilent Bioanalyzer 2100 and QuBit high-sensitivity dsDNA assay. Size-selected libraries were subjected to deep sequencing with Illumina HiSeq X ten at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China. ribo– RNA-seq
attributes.tag: GEO Accession
attributes.value: GSM4509043