title: NextSeq 500 sequencing; Vulnerability of drug-resistant EML4-ALK rearranged lung cancer to transcriptional inhibition
design: Vulnerability of drug-resistant EML4-ALK rearranged lung cancer to transcriptional inhibition
library_name: CR1_2_s
library_construction_protocol: H3122 cells were purchased from ATCC. CrizR1 cells were a kind gift of Dr. Christine Lovly, Vanderbilt University RNA was isolated using TRIzol Reagent (Ambion), according to the manufacturer's protocol 1ug of total RNA from each sample was prepared for conversion to a cDNA library using an Agilent Sureselect Stranded kit and size selected.
attributes.tag: Experimental Factor: response to treatment
attributes.value: crizotinib resistant