title: Illumina HiSeq 3000 paired end sequencing; Bovine small and micro RNA expression atlas
design: Micro RNA sequencing of 18 tissues using HiSeq3000
library_name: BTA-DEDJTR-BBM409015438-BC1-miRNA
library_construction_protocol: NEBNext Multiplex Small RNA Library Prep kit (New England Bioloabs Inc)
attributes.tag: assay type
attributes.value: microRNA profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 6
attributes.tag: extraction protocol
attributes.value: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/fm_1560.pdf
attributes.tag: library preparation location
attributes.value: Department of Economic Development, Jobs, Transport and Resources
attributes.tag: library preparation location longitude
attributes.value: 144.96332
attributes.tag: library preparation location latitude
attributes.value: -37.814
attributes.tag: library preparation date
attributes.value: 2017-01
attributes.tag: sequencing location
attributes.value: Department of Economic Development, Jobs, Transport and Resources
attributes.tag: sequencing location longitude
attributes.value: 144.96332
attributes.tag: sequencing location latitude
attributes.value: -37.814
attributes.tag: sequencing date
attributes.value: 2017-02-07
attributes.tag: experiment target
attributes.value: CHEBI_33697
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
attributes.tag: library generation protocol
attributes.value: https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
attributes.tag: read strand
attributes.value: mate 2 sense
attributes.tag: rna purity - 260:280 ratio
attributes.value: 1.94
attributes.tag: rna purity - 260:230 ratio
attributes.value: 1.59
attributes.tag: rna integrity number
attributes.value: not applicable