title: RNA-seq of Candida glagrata: WT strain
design: Six independent yeast cultures (3 for WT and 3 for gln3  strains) were grown overnight in YPD media on a roller drum with constant spinning, then cells were washed twice and suspended in 1 ml of sterile milliQ water. Flasks containing 50 ml of MM, were inoculated at OD600 nm 0.1, and incubated at 30 C with constant shaking (220 rpm) until OD600 nm 0.8 to 1.0 was reached. Cells then were pelleted, rapidly transferred to liquid nitrogen, and stored at -80 C. RNA extraction, cDNA library construction and sequencing were performed by the Beijing Genomics Institute (BGI) (Hong Kong, China).
library_name: Cg_WT_R2