title: GSM3502860: PvGR_I2; Mus musculus; miRNA-Seq
library_construction_protocol: Brain tissue lysates were centrifuged and the supernatant was mixed with Qiazol aliquot and purified using miRNeasy micro kit (Qiagen). This is total RNA sample used for immunoprecipitation. Ribosome-associated mRNA libraries were prepared using TruSeq® Stranded Total RNA Library Prep (Illumina # RS-122-2201) following the manufacturer protocol. In brief, 0.5ug of total RNA was treated with Ribo-zero rRNA removal and cleaned with RNACleanXP. Both cDNA strands were synthesized, Adenylation of 3' end was done and indexing adapters were ligated. Libraries were amplified and the final product was purified using Agencourt AMPure XP (Beckman Coulter, #A63881). Libraries were validated using High sensitivity DNA bioanalyzer kit (Agilent).
attributes.tag: GEO Accession
attributes.value: GSM3502860