title: NextSeq 500 paired end sequencing; Whole-exome sequencing (WES) of single glands from opposite side of two colorectal tumors and WES of colorectal cancer xenografts derived from single cell culture to study tumor growth dynamics
design: Whole-exome sequencing (WES) of single glands from opposite side of two colorectal tumors and WES of colorectal cancer xenografts derived from single cell culture to study tumor growth dynamics
library_name: HCT116_S1_XB_p
library_construction_protocol: Tumor tissue was collected separately from the right and left lesions of the mice LoVo and HCT116 cell lines were cultured under standard conditions (DMEM medium supplemented with 10% Fetal Bovine Serum). A single founding cell was cloned and expanded in vitro until it reached ~6M cells (a T75 flask). Two aliquots of ~1M cells were subcutaneously injected into opposite (right and left) flanks of a nude mouse (NSG mice for HCT117 and SCID mice for Lovo) and tumors were allowed to reach a size of ~1B cells (1cm3) before the animal was sacrificed. illumina Nextera Rapid Capture Expanded Exome Kit
attributes.tag: Experimental Factor: HCT116
attributes.value: cell line
attributes.tag: Experimental Factor: tissue culture of single cell clone
attributes.value: growth condition
attributes.tag: Experimental Factor: xenograft in NGS mouse
attributes.value: xenograft
attributes.tag: Experimental Factor: xenograft specimen
attributes.value: organism part
attributes.tag: Experimental Factor: post xenograft
attributes.value: sampling time point