title: GSM3502915: Unbound Fraction vGluT2 #3 (Batch 2); Mus musculus; RNA-Seq
library_construction_protocol: Mix post nuclear supernatant with Trizol. Biotinylating RNA and capture of biotinylated RNA with Streptavidin C1 Dynabeads 4-TU labeled RNA-seq libraries were prepared using the ScriptSeq RNA-Seq Library Preparation Kit (Illumina # SSV21124) following the manufacturer protocol. In brief, rRNA depleted RNA was fragmented and cDNA was synthesized. 3' end was tagged, cDNA was purified, indexes was added and samples were amplified. The final product was again purified with Agencourt AMPure XP (Beckman Coulter, #A63881) and libraries were validated using High sensitivity DNA bioanalyzer (Agilent).
attributes.tag: GEO Accession
attributes.value: GSM3502915