title: GSM4511998: WT_MPP2_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared using an RNeasy Micro Kit (QIAGEN) from approximately 3,000 to 10,000 cells according to the manufactures' instructions. cDNA was synthesized and amplified using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). Amplified cDNA was purified using Ampure XP beads (Beckman Coulter) to remove primer dimers. RNA-seq libraries were prepared using a Nextera XT Kit (Illumina). The prepared libraries were sequenced on a NextSeq 500 (Illumina) to generate single-end 75 nucleotide reads.
attributes.tag: GEO Accession
attributes.value: GSM4511998