title: GSM3444805: chip_k27ac_iTreg; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were chemically cross linked by 1% formaldehyde. Cell lysates were made by appropriate sonication and protein-DNA complexes were isolated with indicated antibodies (anti-H3K27Ac: AbCam, ab729, anti-Foxp3: AbCam, ab150743) Libraries were prepared according to Illumina's instructions. Briefly, DNA was end-repaired and the blunt-ended, then phosphorylated ends were treated with Taq polymerase and dATP to yield a protruding 3- 'A' base for ligation of NEBNext adapters which have a single 'T' base overhang at the 3' end. After adapter ligation, DNA was PCR amplified with NEBNext index primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel, and quantitated by Qubit (Invitrogen).
attributes.tag: GEO Accession
attributes.value: GSM3444805