title: GSM3446015: WT Meckel's cartilage replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Laser Capture Microdissection was used to isolate tissues from Meckels cartilage, mandibular bone and mandibular condylar cartilage, respectively. RNA was isolated with Arcturus Picopure RNA Isolation Kit as described by the manufacturer and eluted in a final volume of approximately 16 µl of elution buffer. RNA quality and concentration was assessed using the Agilent RNA Pico kit and Agilent Bioanalyzer 2100 as described by the manufacturer. Libraries were prepared with NuGEN Ovation RNA-Seq System v2 (amplification) and Illumina Nextera XT Library Prep kit. Fragments were sequenced using paired end reads (2×100 bp) on the Illumina HiSeq platform.
attributes.tag: GEO Accession
attributes.value: GSM3446015