title: 16s amplicon: loosely adhered bacterial community, initially late stage, 2 g ration, no larvae
design: Nucleic acids were extracted using MOBIO PowerSoil Powerlyzer extraction kits. Direct amplification of the V4 region of the bacterial/archaeal small subunit ribosomal RNA gene with primer set 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT). Sequenced with Illumina MiSeq flow cell in a 2x250 bp paired-end format.
library_name: BAC_LA_1M2G0L_E_37