title: V3-V4 metataxonomy of summer propolis from beehive
design: Total DNA was extracted from three sub-samples of the finely chopped propolis sample stored at -80C, using the PowerBiofilm DNA Isolation Kit Sample. The concentration of extracted DNA was assessed using Qubit. Samples of total DNA from propolis were sent to the external services IGA Technology Services (Udine, Italy) for 16S rDNA and to BMR Genomics (Padova, Italy) for ITS rDNA sequencing. NGS sequencing was performed with a MiSeq instrument (Illumina, California) with a paired-ends 2x300 bp sequencing approach, following the service provider internal protocol. For bacterial community, the V3-V4 hypervariable region of the 16S rDNA was amplified and sequenced using primers 341F (5-CCTACGGGNGGCWGCAG-3) and 805R1 (5-GACTACHVGGGTATCTAATCC-3). For fungal community analysis, the ITS2 portion of the ITS region was amplified and sequenced with primers ITS3 (5-GCATCGATGAAGAACGCAGC-3) and ITS4.
library_name: BP11_16S