title: GSM3332310: D8ANAcere-3 logarithmic phase replicate 3; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: The RNA protocol extraction used was adapted from (Moyrand et al., Eukaryotic Cell, 2008). Briefly, the samples were lyophilized overnight. RNAs were extracted following a protocol divided in 3 parts: i) an extraction step was performed in Trizol reagent (Invitrogen), ii) a second step by adding Chloroform (Invitrogen) iii) the last step was an overnight Isopropanol precipitation at -20°C. We used 1 μg of total RNA to purify polyadenylated mRNAs and to build an RNA library, using TruSeq Stranded mRNA Sample Prep Kit (Illumina, #RS-122-9004DOC) as recommended by the manufacturer. Directional library are checked for concentration and quality on DNA chips with the Bioanalyser Agilent. More precise and accurate quantification is performed with sensitive fluorescent-based quantitation assays ("Quant-It" assays kit and QuBit fluorometer, Invitrogen).
attributes.tag: GEO Accession
attributes.value: GSM3332310