title: GSM3711861: Kmt2d+/+ 10-5 Undiff D0 rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIZOL reagent and RNA Clean & Concentrator-5 kit (Zymo Research). After DNAse treatment, RNA was assessed for quantity and quality using Qubit RNA BR Assay Kit (ThermoFisher Scientific) and RNA 6000 Nano Kit on the Agilent Bioanalyzer 2100 system (Agilent Technologies). mRNA was purified using NEBNext Poly(A) mRNA Magnetic Isolation Module for Illumina (New England Biolabs). Sequencing libraries were generated using NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs) and were validated using the Agilent High Sensitivity DNA assay on the Agilent Bioanalyzer 2100 system and quantified by NEBNext Library Quant Kit for Illumina (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM3711861