title: GSM2858933: S11_sham_input_1; Rattus norvegicus; ChIP-Seq
library_construction_protocol: Sciatic nerves were subjected to MNase-ChIP with anti-H3K27me3 antibody from Active Motif (AM39155) as described previously with minor modification (Ma et al., 2015). After incubation with Dynabeads Protein G (Invitrogen, 10004D), ChIP samples were washed with washing buffer 1 (WB1, 50 mM Tris-HCl, pH7.5; 10 mM EDTA; 125 mM NaCl) once, WB2 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 250 mM NaCl) once, and WB3 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 500 mM NaCl) twice. The samples then eluted at 65°C with elution buffer (50 mM NaCl; 50 mM Tris-HCl, pH 7.5; 5mM EDTA; 1% SDS) for 15 min. Libraries were prepared from ChIP DNA, and respective inputs, using the Illumina TruSeq ChIP Sample Preparation Kit.
attributes.tag: GEO Accession
attributes.value: GSM2858933