title: GSM2859462: 925L_NC_mono_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted with TRIzol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. The RNA concentration was determined using the Quant-iTTM RiboGreen® RNA Assay Kit The cDNA libraries were prepared from 2 ng of total RNA and 1 μl of a 1:50,000 dilution of external RNA control consortium (ERCC) spike-in control mix (Thermo Fisher Scientific) using SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: 1) use of 20µM template-switching oligos (TSO), 2) use of 250 pg of cDNA with 1/5 reaction mixtures of Illumina Nextera XT kit.
attributes.tag: GEO Accession
attributes.value: GSM2859462