title: GSM2175341: HeLa_S3 (WGSBS).; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN) Library construction was performed using the EpiGnome/TruSeq DNA Methylation Kit. Briefly, genomic DNA (50-100 ng) was denatured and bisulfite converted in a reaction containing 0.5 ng of unmethylated lambda DNA (Promega) as a control. Following purification, the BS converted DNA was hybridized with oligonucleotides consisting of random hexamers linked to Illumina P5 adapter sequences and strand replicated using EpiGnome polymerase. Adapter-ligated DNA molecules were enriched by 10 cycles of PCR.
attributes.tag: GEO Accession
attributes.value: GSM2175341