title: GSM3035975: ID8-PW2 (duplicate-1); Mus musculus; RNA-Seq
library_construction_protocol: Cells (10^7) were lysed and RNA was extracted according to manufacturer's protocol (Qiagen RNeasy Mini kit). Total RNA was fractionated by size using ethanol concentration manipulations. The large RNA fraction (>200 nt) was fragmented prior to library construction. Ribosomal RNA was reduced by duplex specific nuclease (DSN) following limited hybridizations of both fractions and then amplified to add barcodes for multiplexing on the Illumina HiSeq2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM3035975