title: GSM4368385: Gr5a_SD_3days 1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 2-3 biological replicates were used for each condition, and each replicate consisted of 300 heads. For tissue collections, briefly heads were collected using pre-chilled sieves in liquid nitrogen on dry ice. Frozen heads were then lysed. From the lysate total RNA was extracted by TRIzol LS Reagent and used as input. The remainder of the lysate was incubated with Dynabeads protein G  to preclear samples for 2 hours and subsequently incubated with Dynabeads protein G coated with an anti-Flag antibody. The lysate-beads mixture was incubated at 4°C with rotation for 2 hours, then washed in buffer. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent. Samples were ribo-depleted, DNase treated, and libraries were prepared using the Nugen Ovation SoLo RNA-Seq System for Drosophila (0502) by the University of Michigan sequencing core facility. Library concentrations were determined by qPCR.
attributes.tag: GEO Accession
attributes.value: GSM4368385