title: epiGBS of Taraxacum officinale: leaf tissue
design: Leaf tissue was collected in 5-week old plants from a standardized leaf (leaf #6), flash frozen, stored at -80C. DNA was isolated, after TissueLyser tissue homogenization using steel beads, using Machery-Nagel Nucleosipin Plant II kits with cell lysis buffer PL1. EpiGBS library preparation was done as described in van Gurp 2016 (Nature Methods 13(4):322) using Pst1 digestion. During epiGBS library preparation samples were individually barcoded and subsequently pooled for multiplexed sequencing. Two pooled sequencing libraries were created, one consisting of 96 individual samples (lib1, 96-plex) and one consisting of 82 individual samples (lib2, 82-plex). Sequencing output files are provided per individual sample after de-multiplexing.
library_name: epigbs_lib1_CCAG_ATTG