title: GSM4018046: BY4741 mip6D yeast cells, 30ºC , H4K12ac ChIPseq 5; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: For RNAseq samples, a 10 mL aliquote is collected from each culture, washed twice with pure water and celular pellet was then frozen in liquid nitrogen. Total RNA was extracted with hot acid phenol protocol. For ChIPseq samples, a crosslinking reaction was performed with 1% formaldehyde for 15 minutes and the reaction was then stopped with 125 mM glycine for then washing three times the pellet with TBS buffer and frozen in liquid nitrogen. ChIP experiment was performed with 1 microL per sample of H4 (ab10158) or H4K12ac (39165 Active Motif) antibodies bound to magnetic beads and incubated for 2 hours before IP. It followed the genomic DNA extraction with phenol protocol. For RNA: TruSeq Stranded Total RNA Sample Prep . For ChIPseq: ChIPseq specific library TruSeq for RNAseq and ChIPseq library for ChIPseq
attributes.tag: GEO Accession
attributes.value: GSM4018046