title: GSM4017100: Detroit HGF 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were centrifuged for 5 min at 2000 rpm and 4°C. Total RNA was isolated using a commercially available kit (RNeasy Mini Kit, Qiagen). Cells were lysed using 350 µl RLT lysis buffer, and syringe and needle were used for homogenization. Further processing was performed as described in instructions provided by the manufacturer. RNA libraries were prepared for sequencing using TruSeq Stranded mRNA kit (Illumina) with 600 ng total RNA as input for mRNA capture with oligo-dT beads.