title: 16S rRNA gene sequence of sheep faecal samples
design: PCR amplification and sequencing was performed by the Australian Genome Research Facility. Briefly, PCR amplicons were generated using the primers 27F [AGAGTTTGATCMTGGCTCAG] and 519R [GWATTACCGCGGCKGCTG] targeting the V1_V3 region of the 16S rRNA gene, and using AmpliTaq Gold 360 mastermix (Life Technologies, Australia) for the primary PCR.  A secondary PCR to index the amplicons was performed with TaKaRa Taq DNA Polymerase (Clontech). The resulting amplicons were measured by florometry (Invitrogen Picogreen) and normalised.  The eqimolar pool was then measured by qPCR (KAPA) followed by sequencing on the Illumina MiSeq (San Diego, CA, USA) with 2 x 300 base pairs paired-end chemistry. All samples analysed were included in the same sequencing run.
library_name: Sample 1