title: GSM4198807: WT -Cr (untreated)_R2; Candida albicans; RNA-Seq
library_construction_protocol: Total RNA was extracted by the hot phenol method. One microgram of total RNA was used for library preparation by the PAT-seq method. Library construction was by the PAT-seq approach (Harrison et al 2015) Illumina directional RNA-seq: The libraries provided were purified using AMPure beads to remove any contaminating primers etc., and  examined using Agilent Bioanalyzer. These showed libraries with mean size of 243bp for both 18-04608  and 18-04609. Quantitation was also performed using Qubit and qPCR. From size adjusted qPCR the  libraries were 18.4nM after purification. c-Bot Hybridization using 14pM of each library pool and on-board cluster  generation. 150bp SR sequencing using Illumina HiSeq Rapid Mode and HiSeqV2  chemistry. Illumina Protocol 15035788 Rev D, Apr 2014
attributes.tag: GEO Accession
attributes.value: GSM4198807