title: Amplicon sequencing of the 16S rRNA hypervariable region V4 of phyllosphere endophytic bacterial communities in Populus trichocarpa in Teanaway
design: Amplicon sequencing of the V4 region of the 16S rDNA gene using the primer set515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806rB (5-GGACTACNVGGGTWTCTAAT-3), along with 100 M of sequence-specific peptide nucleic acid (PNA) clamps for to reduce host-derived contaminations from chloroplast and mitochondria. The Exo-SAP-IT kit(Affimatrix) was used to clean the PCR products, and amplicons were tagged with Illumina sequencingprimers following the standard Illumina protocol for amplicon library preparation. The libraries werethen sequenced on the Illumina MiSeq sequencer using the v2 2x300 bp read kit by the Joint GenomeInstitute.
library_name: A7-Tea2