title: GSM3445017: Dux_WT_late_2-cell_replicate2; Mus musculus; RNA-Seq
library_construction_protocol: Dux WT/KO 1-cell and 2-cell embryos were collected by in vitro fertilization (IVF). 1-cell embryos were collected at 12 hours post fertilization (hpf) and 2-cell embryos were collected at 30 hpf. SMARTer Ultra Low Input RNA cDNA preparation kit (Clontech, 643890) was used for reverse transcription and cDNA amplification (11 cycles).  cDNA were then fragmented, adaptor-ligated and amplified using Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer's instructions.  Single-end 100bp sequencing was performed on a HiSeq 2500 sequencer (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3445017