title: GSM4199214: mut-16(pk710) Mutant Gravid Adults mRNA 20C, Rep 3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: sample collection: Whole worms were flash frozen in liquid nitrogen prior to Trizol/chloroform extraction RNA was depleted of rRNA using RiboZero and enriched for >200nt sizes with Zymo RNA clean and concentrator-5. Libraries were then prepared according to the NEBNext® Ultra™ II Directional RNA kit protocol. Size selection and library purification was done using AMPure XP beads.
attributes.tag: GEO Accession
attributes.value: GSM4199214