title: GSM2857609: NRO-seq in SKNMC cell line; Homo sapiens; OTHER
library_construction_protocol: Freshly extracted nuclei were incubated 30 minutes at 30°C in presence of BrUTP (Roche). Nuclear RNA was extracted using the NucleoSpin miRNA kit (Clontech) and nascent RNA was immunoprecipitated with an anti-BrdU monoclonal antibody (Santa Cruz). Following washes, purified RNA was isolated using the NucleoZOL extraction kit (Clontech). Nascent RNA samples were treated with RiboGone oligonucleotides to remove ribosomal RNA (Clontech). Illumina compatible sequencing libraries were constructed using SMARTer Stranded Total RNA sample Prep Kit (Clontech) according to the manufacturer's instructions
attributes.tag: GEO Accession
attributes.value: GSM2857609