title: GSM2936713: Input of ChIPseq for HP1a in Cbx1-/- Cbx3-/-  mES cells, replicate 1; Mus musculus; ChIP-Seq
library_construction_protocol: 10^7 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 1% of chromatin was set aside as Input control. The chromatin was incubated with Strapdavidin coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ostapcuk et al, 2017. Libraries were prepared using the NEB Next DNA library prep kit according to manufacturers instructions. For barcodes, see sample characteristics.
attributes.tag: GEO Accession
attributes.value: GSM2936713