title: GSM4142809: PND28-17; Heterocephalus glaber; RNA-Seq
library_construction_protocol: Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. RNA concentration was determined on a Nanodrop. Ribosomal RNA was subtracted by hybridization from total RNA samples using the RiboZero Magnetic Gold H/M/R Kit (Illumina) using 100ng total RNA and a modified protocol scaled for lower input (90ul magnetic bead stock, and 2 ul removal solution in a 20ul reaction). The rRNA-depleted RNA samples were cleaned up by ethanol precipitation. TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM4142809