title: GSM3032949: PDAC-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from samples using the PicoPure RNA isolation kit (ThermoFisher) following manufacturer's instructions, including on column DNAse treatment using RNase-Free DNase I (Qiagen). RNA sequencing libraries were prepared with Kapa RNA HyperPrep kit with RiboEase (Kapa Biosystems, Cat KR1351) according to the manufacturer's protocol. Sequencing run was performed in the single read mode of 51cycle of read1 and 7 cycles of index read using Illumina HiSeq 2500 with HiSeq SBS V4 Kits. Real-time analysis (RTA) 2.2.38 software was used to process the image analysis and base calling.
attributes.tag: GEO Accession
attributes.value: GSM3032949