title: GSM4200337: null_input; Mus musculus; ChIP-Seq
library_construction_protocol: pkd1 and null cells were cross-linked with 1% formaldehyde for 10 min at RT. The crosslink was stopped by adding 1/20 volume of 2.5 M glycine. Cells were washed with PBS and harvested using ChIP lysis buffer. Cells were then sonicated to obtain fragments (100-500 bp) with Bioruptor Sonicator. Immunoprecipitation was performed with H3K27ac antibody (Abcam cat# ab4729). After elution and reversal cross-linking, DNA was purified and sequenced on BGISEQ-500. Construction of ChIP-seq library was completed by BGI company (Shenzhen,China).
attributes.tag: GEO Accession
attributes.value: GSM4200337