title: GSM2695541: Gata4(fl/fl);Ptf1a(CreEr/+) GPFC-28b-7; Mus musculus; RNA-Seq
library_construction_protocol: RNA preparation:  pancreas was homogenized in 4 M Guanidine Thiocyanate, 0.1 M Tris HCl pH 7.5, 1% 2-mercaptoethanol with a Tekmar Tissuemizer.  RNA was isolated from an aliquot of the homogenate by addition of N-Lauroylsarcosine sodium salt to 0.5%, Potassium Acetate pH 5.5 to 0.1 M, and Acetic Acid to 0.08 M, followed by addition of 0.75 volume ethanol while vortexing.  RNA was allowed to precipitate from the solution at -20 C for at least 1 hour, collected by centrifugation at 12,000 g for 5 minutes, and the RNA pellet resuspended in Trizol (equal volume to starting homogenate).  Subsequent steps were as per manufacturer's protocol (Life Technologies) or with Zymo Research Direct-Zol RNA mini-prep. RNA libraries were prepared for sequencing using standard Illumina True-seq protocols
attributes.tag: GEO Accession
attributes.value: GSM2695541