title: GSM2936320: 4T1; Mus musculus; RNA-Seq
library_construction_protocol: Cell pellet was snap-frozen in liquid nitrogen and shipped to BGI for RNA extraction After extract total RNA and treated with DNase I, Oligo(dT) were used to isolate mRNA.Mixed with the fragmentation buffer, the mRNA were fragmented. Then cDNA was synthesized using the mRNA fragments as templates. Short fragments were purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments were selected for the PCR amplification. Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library.
attributes.tag: GEO Accession
attributes.value: GSM2936320