design: Total RNA isolated from aphids collected at 24, 48, 72 and 96h post-infestation using the RNeasy Plus Kit (Qiagen, GmbH, Hilden, Germany) was quality-checked, and equal amounts of total RNA (500ng) from each post-infestation time were pooled to form six treatments, including: Diuraphis noxia biotype 1 fed plants containing Dn0, Dn4 or Dn7, and Schizaphis graminum fed plants containing Dn0, Dn4, or Dn7. 1 ug of total RNA (100ng/ul) from each sample was used for library preparation with the Illumina TruSeq RNA Library kit (Illumina Inc., San Diego, CA) per the manufacturer's recommendations. These libraries were validated, and a portion of each was diluted to a 10nM concentration. Samples were separately barcoded for multiplexing, and libraries from all 18 samples (3 treatments, 2 aphid species, and 3 biological replicates) were combined into two pools (each loaded in 1 lane) of 9 libraries each. A 1 x 100 bp single-read sequencing run was performed using an Illumina TruSeq single-read clustering Kit v3 and Illumina TruSeq SBS-HS v3 sequencing chemistry on an HiSeq 2500 Illumina sequencer. Library preparation and sequencing were conducted at the University of Kansas Medical Center in Kansas City, KS.
library_name: 18R2D7-3