title: mRNA sequencing of  medicago sativus
design: 5-day-old seedlings were pretreated with or without 10% HRW for 12 h with or without another 12 h Cd stress. After various treatments, the seedling roots were harvest. For each treatment group, one mixed sample form three replicate experiments was used for RNA extraction. Total RNA was extracted using CTAB following the manufacturers protocol. RNA integrity was confirmed by Agarose gel electrophoresis.
library_name: 3