title: RNA-sequencing of Danio rerio: scoliosis trunk
design: Total RNA extraction was preformed using the Qiagen RNeasy kit following the manufacturer's instructions (Qiagen). RNA sample quality was assessed using an Agilent Bioanalyzer. Library prep and subsequent sequencing was performed by The Centre for Applied Genomics at The Hospital for Sick Children (SickKids) using the NEBNext stranded RNA
library_name: ptk7_mutant_4