title: GSM3768096: PBS MOCK 2M CD45 2 biological replicate 2 [ATAC-seq]; Mus musculus; ATAC-seq
library_construction_protocol: DNA was isolated as previously described (Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y. & Greenleaf, W. J. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat. Methods 10, 1213–1218, 2013). 50,000 cells were used for the transposition reaction, that was carried out for 4h at 37°C. Library fragments were amplified using 1× NEBnext PCR master mix and 1.25 μM of custom Nextera PCR primers. using the following PCR conditions: 72 °C for 5 min; 98 °C for 30 s; and thermocycling at 98 °C for 10 s, 63 °C for 30 s and 72 °C for 1 min - this was done for 13 cycles. Transposed DNA fragments were then PCR amplified for 13 cycles and purified by the ChargeSwitch PCR clean-up kit (Invitrogen)
attributes.tag: GEO Accession
attributes.value: GSM3768096