title: Illumina MiSeq paired end sequencing
design: Illumina sequencing of library 7067784, constructed from sample accession ERS226938 for study accession ERP015660.  This is part of an Illumina multiplexed sequencing run (9603_1).  This submission includes reads tagged with the sequence ACAGTG.
library_name: 7067784
library_construction_protocol: qPCR only
attributes.tag: BISULFITE_CONVERSION_PERCENT
attributes.value: 99.9%
attributes.tag: BISULFITE_CONVERSION_PROTOCOL
attributes.value: QIAGEN EpiTect Bisulfite - FFPE protocol
attributes.tag: DNA_PREPARATION_ADAPTOR_LIGATION_PROTOCOL
attributes.value: TruSeq DNA LT Sample Prep-Kit Set A (July 2012, Illumina, FC-121-2001) - 30C for 10 minutes
attributes.tag: DNA_PREPARATION_ADAPTOR_SEQUENCE
attributes.value: AD005 (GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG)
attributes.tag: DNA_PREPARATION_ADAPTOR_SOURCE
attributes.value: Methylated adaptors (Illumina ME-100-0010)
attributes.tag: DNA_PREPARATION_FRAGMENT_SIZE_RANGE
attributes.value: 280bp average size (50bp-650bp)
attributes.tag: DNA_PREPARATION_INITIAL_DNA_QNTY
attributes.value: 1ug
attributes.tag: DNA_PREPARATION_POST-LIGATION_FRAGMENT_SIZE_SELECTION
attributes.value: 450-550bp average size - AMPure XP beads (Agencourt)
attributes.tag: EXPERIMENT_TYPE
attributes.value: oxDNA Methylation
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: AllPrep DNA/RNA Mini Kit (QIAGEN 80204)
attributes.tag: EXTRACTION_PROTOCOL_SONICATION_CYCLES
attributes.value: 200
attributes.tag: EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
attributes.value: Covaris E210
attributes.tag: LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
attributes.value: PCR Primer Cocktail - TruSeq DNA LT sample preparation kit (Illumina, FC-121-2001)
attributes.tag: LIBRARY_GENERATION_PCR_NUMBER_CYCLES
attributes.value: 10 cycles
attributes.tag: LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
attributes.value: Pfu Turbo Cx Hotstart DNA polymerase
attributes.tag: LIBRARY_GENERATION_PCR_PRIMER_CONC
attributes.value: PCR Primer Cocktail - TruSeq DNA LT sample preparation kit (Illumina, FC-121-2001) (1.5ul/50ul reaction)
attributes.tag: LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
attributes.value: AMPure XP beads (Agencourt) 1:1
attributes.tag: LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
attributes.value: PCR Primer Cocktail - TruSeq DNA LT sample preparation kit (Illumina, FC-121-2001)
attributes.tag: LIBRARY_GENERATION_PCR_TEMPLATE_CONC
attributes.value: 5-10ng/ul
attributes.tag: LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
attributes.value: 95C for 2min; 10 cycles of: 95C for 30 sec, 60C for 20 sec, 72C for 45 sec; 72C for 5min; hold at 4C