title: UCE target enrichment of Ambloplites constellatus
design: Illumina libraries were constructed using the Kapa Hyper Prep Kit (Kapa Biosystems) with dual-indexing barcodes. All reactions followed manufacturer protocols, except reaction sizes were scaled to half the volume indicated by the manufacturer. After library amplification, samples were pooled in equimolar ratios in sets of six to eight samples. Target enrichment of UCE loci was performed on each pool using the MYbaits 0.5k Actinopterygian UCE capture kit (MYcroarray), originally described in Faircloth et al. (2013), following manufacturers protocols. Pools were then amplified and cleaned using 1618 PCR cycles following procedures outlined in Faircloth et al. (2013). All pools were combined in equimolar proportions, and were sequenced either at the University of Georgia Genomics Institute, or the Oklahoma Medical Research Institute, using an Illumina HiSeq or MiSeq Sequencer.
library_name: Ambloplites_constellatus_YPM15709