title: GSM2174866: 3xFlag-Ago1 WT (spy797); Schizosaccharomyces pombe; ncRNA-Seq
library_construction_protocol: Nuclei were isolated and sRNA associated with Ago1 were immunoprecipitated with Flag antibody Small RNA libraries were constructed as previously described (Halic and  Moazed, 2010). Briefly, 21-24nt RNA was size-selected on a 17.5% polyacrylamide/7M  urea gel and ligated to a 3’ adapter. The ligated species were size-selected on a 17.5%  polyacrylamide/7M urea gel and ligated to a 5’ adapter. RNA was then reverse  transcribed into cDNA and PCR-amplified in a two-step process. Amplified cDNA was  gel-purified and sequenced on an Illumina High-Seq platform.
attributes.tag: GEO Accession
attributes.value: GSM2174866