title: GSM3873062: 68_RNA_KO_Th1_rep1; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq, total RNA was isolated using the RNeasy kit (Quiagen), 500 ng of RNA were subsequently used to prepare (polyA-)RNA-seq libraries by a combination of NEBNext RNA library prep kit (New England BioLabs) and Ovation SP Ultralow DR Multiplex system (Nugen) following the manufacturer's protocol. Libraries were sequenced for 50 single read cycles on HiSeq 2500 following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM3873062