title: microbial diversity of ornamental fish aquarium water sample D2.V4
design: target_taxon: Bacteria  target_gene: 16S rRNA  target_subfragment: V4  pcr_primers: 518F 5'CCAGCAGCYGCGGTAAN'3, Vib2 r-5'-GAAATTCTACCCCCCTCTACAG-3'  pcr_cond: initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;30 cycles
library_name: VS-454-MBL:assay:D2.V4.10_Bacteria