title: GSM3106742: WT_F_1; Mus musculus; RNA-Seq
library_construction_protocol: The total RNA was isolated from mice liver using a RNeasy kit from Qiagen. The mRNA was separated from total RNA by Oligo(dT) magnetic beads, and cDNA synthesized by reverse transcription with N6 primer. Adaptors were ligated to the sticky 3' end of the cDNA. Two specific primers were used in PCR to amplify the ligation products. Denature the PCR product by heating and the single strand DNA is cyclized by splint oligo and DNA ligase.
attributes.tag: GEO Accession
attributes.value: GSM3106742