title: GSM2694180: FAIRE-seq RPMI-8226; Homo sapiens; FAIRE-seq
library_construction_protocol: For FAIRE-seq, about 5M cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Formaldehyde was quenched  by 0.2M glycine. Nuclei were isolated and sonicated. Sonicate was extracted with phenol–chloroform–isoamyl alcohol (25:24:1) and reverse crosslinked. DNA were purified and size selected before Illumina libraries generation. Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (E7645, NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
attributes.tag: GEO Accession
attributes.value: GSM2694180