title: Assessing Eukaryotic Biodiversity in the Florida Keys National Marine Sanctuary Through Environmental DNA 18S Metabarcoding
design: This protocol is aimed at amplifying the 18S rRNA hypervariable region 9 (18S V9) in eukaryotes with a focus on microbial eukaryotes. The primers (1391F, EukBr, and Mammal_block_I-short_1391f ) utilized in this protocol are based on the primer utilized in Amaral-Zettler et al 2009 and the Earth Microbiome Project (EMP)
library_name: FKNMS_LK_20150414_0_1_18S