title: Ribosome-IP of mouse PrL
design: Mice  PrL were dissected in ice-cold PBS. The brain tissue was homogenized in 1 ml Supplemented Hybridization Buffer  containing 1 mM DTT, 1 protease inhibitors, 200 units/ml RNase inhibitor, 100 g/ml cycloheximide, and 1 mg/ml heparin. The supernatant was incubated with 10 g anti-HA antibody and 100 l Dynabeads Protein G for 12 hrs. Purified mRNA were eluted from the Dynabeads using TRIzol LS according to the manufacturers instructions with the inclusion of a DNase digestion step.  mRNA was enriched using NEB Next Poly(A) mRNA Magnetic Isolation Module (E7490S, NEB, Ipswich, MA, USA). Library was prepared with NEB Next Ultra RNA Library Prep Kit (E7530S) and sequenced on a HiSeq 4000 (Illumina) by Novogene Technology Co. Ltd (Beijing, PRC).
library_name: PV_Sal-1