title: GSM3333319: CF_Nontreated_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated with Qiazol Lysis Reagent (Qiagen Inc, Valencia, CA), cleaned-up using the RNeasy Mini Kit (Qiagen Inc), and eluted in 30 µl of RNase-free water (Qiagen Inc). RNA-Seq libraries were generated using TruSeq stranded Total RNA Ribo-Zero human gold kit (Illumina, San Diego, CA) and the quality of resulting libraries was assessed using Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). Sequencing was carried out using an Illumina HiSeq 2500 Rapid Run flowcell – 2x100bp run.
attributes.tag: GEO Accession
attributes.value: GSM3333319