title: H3K9me3 ChIP-Seq of Plasmodium falciparum: gametocyte
design: Parasites were purified using a percoll gradient, incubated in lysis buffer containing 1% Igepal CA-360 for 10 min at RT and passed through a 26 G 1/2 inch needle 15 times. Parasites were then crosslinked for 10 min with 1% formaldehyde at 37C. Chromatin was sheared on a Covaris S220 for 6 min (5% duty cycle, 140 intensity peak incident power, 200 cycles per burst) and diluted 1:1 in ChIP buffer. ChIP was performed O/N using 2 ug of antibody (or no antibody for control).
library_name: Gametocyte_H3K9me3