title: GSM3032598: P5WT 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from brain tissue samples using Trizol Reagent. RNA was purified using the Direct-Zol RNA Miniprep Kit (Zymo Research, CA, USA; Catalogue No. R2050) according to the manufacturer's recommendations. Library Prep was performed by the Oxford Genomics Centre (Oxford, United Kingdom). mRNA was selected from total RNA and converted to cDNA. dUTP was incorporated during second strand cDNA synthesis. CDNA was end-repaired, A-tailed and adaptor ligated. Uridine digestion was performed before amplification of samples. Prepared libraries were size selected and multiplexed and paired-end sequencing was performed across 2 lanes of a flow cell on the Illumina HiSeq 4000 platform.
attributes.tag: GEO Accession
attributes.value: GSM3032598