title: GSM4292881: MDSC-2; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from the purified MDSCs using Quick-DNA Miniprep Plus Kit (Zymo Research, Irvine, CA). A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer's instructions. Then these DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research). And the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X). Library concentration was quantified by Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was checked on Agilent Bioanalyzer 2100 system. 
attributes.tag: GEO Accession
attributes.value: GSM4292881