title: NextSeq 500 sequencing; RNA-seq of mouse Lgr5+ intestinal stem cells and progenitors following Lkb1 deletion against controls
design: RNA-seq of mouse Lgr5+ intestinal stem cells and progenitors following Lkb1 deletion against controls
library_name: Sample 9_s
library_construction_protocol: Approximately 10,000-90,000 Lgr5-GFPHi ISCs or Lgr5-GFPLow progenitors from 3 pairs of WT or Lkb1Lgr5-KO mice 5 days after tamoxifen injection were isolated by flow cytometry. Tamoxifen induction on Lgr5GFP-Cre/+;Lkb1+/+;R26Td/+ mouse leads to the activation of TdTomato lineage tracing while Lkb1 remains wildtype (Lkb1-WT) in intestinal Lgr5+ stem cells and their progeny in comparison to tamoxifen induction on Lgr5GFP-Cre/+;Lkb1fl/fl;R26Td/+ mouse leads to the activation of TdTomato lineage tracing and inactivation of Lkb1 (Lkb1-KO) in intestinal Lgr5+ stem cells and their progeny. Total RNA was extracted using TRI Reagent (MRC, TR118) Ovation Universal RNA-Seq System kit was used for lllumina library preparations (NuGEN Technologies., CA, USA). Purified total RNAs were used for ribosomal removal as outlined in the kit manual. Libraries were purified with AMPure XP beads (Beckman Coulter Inc., MA, USA).
attributes.tag: Experimental Factor: phenotype
attributes.value: Lkb1 knockout; Lgr5-GFP low