title: GSM4452166: ShEE3; Rattus norvegicus; RNA-Seq
library_construction_protocol: brain collection and total RNA extraction protocol: Rats were lightly anesthetized with isoflurane and decapitated. Brains were dissected and flash frozen in isopentane on dry ice and stored at −80°C. Sections of 200-micron thickness were cut and mounted on gelatin-coated glass slides. MPOA from Bregma −0.24 to −0.72 mm was dissected bilaterally under a dissecting microscope. Total RNA was extracted with the Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hercules, CA, USA). The integrity, purity and concentration of RNA were measured. Purified total RNA was stored at −80°C until processed. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4452166