title: GSM2936265: Ago2IP_1-miR146a; Mus musculus; RIP-Seq
library_construction_protocol: Afte proteinase K treatment RNA was isolated using Phenol-chloroform isoamyl extraction Libraries were prepared using Clonetch SMARTer Stranded RNA-Seq Kit, starting with 8μl of the IPed RNA. The manufacturer's protocol was followed, using 4 minutes for RNA fragmentation, double SPRI bead clean-up after reverse transcription and 20 PCR cycles for amplification. The resulting libraries were sequenced using Illumina Hiseq2000 instrument with 50 base pair single read sequencing mode.
attributes.tag: GEO Accession
attributes.value: GSM2936265