title: GSM3503330: 8_RNA-seq with hspA-atrR allele present; Aspergillus fumigatus Af293; RNA-Seq
library_construction_protocol: Mycelia that formed as a biofilm on the top were collected (∼100 mg) and  ground into a fine powder in liquid nitrogen using a mortar and pestle. Total RNA was then isolated from the ground mycelium using the Trizol reagent (Ambion) according to the manufacturer's instructions. Total RNA was isolated and purified using an RNeasy minikit (Qiagen). Traces of contaminating genomic DNA were eliminated by treating the RNA with turbo DNase followed by a DNase inactivation reagent (both reagents from Ambion).  Sequencing libraries were generated using the Illumina TruSeq Stranded mRNA sample preparation kit (Illumina, Inc., San Diego, CA) and following Illumina's sample preparation guide, started with 500ng of input total RNA.
attributes.tag: GEO Accession
attributes.value: GSM3503330