title: GSM3446048: wild-type retina_AAV viral vector bearing pre-miR-204 (AAV2/8.CMV.miR204), biol rep2 (animal n. 2, Left Eye OS); Mus musculus; RNA-Seq
library_construction_protocol: Extraction of total RNA was performed according to the manufacturer's instructions (miRNeasy kit (Qiagen, Inc., Hilden, Germany). Libraries were prepared according manufacturer's instructions (TruSeq RNA Sample Preparation kit, Illumina) starting from 4 μg of total RNA. Quality control of library templates was performed using a High Sensitivity DNA Assay kit (Agilent Technologies) on a Bioanalyzer (Agilent Technologies). The Qubit quantification platform (Qubit 2.0 Fluorometer, Life Technologies) was used to normalize samples for the library preparation. The amplified fragmented cDNA of ~200 bp in size were sequenced in paired-end mode using the HiSeq 1000 (Illumina) with a read length of 2×100 bp
attributes.tag: GEO Accession
attributes.value: GSM3446048