title: 16S-seq of water in Spain temp 50
design: 1st The DNA of the water samples was extracted with the DNeasy PowerWater kit (Qiagen, GER). 2nd Amplification and preparation of amplicons libraries with the following primers (5'CCTACGGGNGGCWGCAG-3 '), (5'GACTACHVGGGTATCTAATCC-3'), to allow amplification of the V3-V4 regions of the 16S rRNA gene. 3rd sequencing, performed through the Illumina MiSeq platform using the "MiSeq Reagent Kit v3" Kit, in a 2 x 300 bp paired-end system.
library_name: ES_PR