title: GSM4512057: Mock_PR8-1; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were produced from whole lungs from PR8-infected mice. The single cells were then enriched for monocytes using Miltenyi Biotech's monocyte sorting kit. Briefly, single cells were incubated with antibodies against non-monocyte markers and subsequently incuabted with beads that bind the primary antibodies. Using LS columns, the non-monocyte cells were captured and retained within the column while the unbound monocytes eluted through and were collected for RNA-sequencing. The purity and viability of the monocytes was assessed via flow cytometry post-sorting.
attributes.tag: GEO Accession
attributes.value: GSM4512057