title: 16S rRNA amplicon fragment from bulk soil samples of the Brazilian Atlantic Rainforest
design:  Environmental DNA was isolated from bulk soil from the Brazilian Atlantic Rainforest using the MoBio PowerSoil® DNA Isolation kit (MoBio, USA). This was used as control to compare against respective mycosphericsoil below a fruiting body (mushroom). Pyrosequencing analysis was based on sequencing the specific region V4 of the highly conserved 16S rRNA gene, used here to identify soil bacterial groups interacting with fungal structures in the mycosphere. The extracted DNA samples were amplified with primers flanking the 16S rDNA V4 region using 520F (5'-AYT GGG YDT AAA GNG-3') forward primers and a mixture of 802R (5'-TAC CRG GGT HTC TAA TCC-3', 5'-TAC CAG AGT ATC TAA TTC-3', 5'- CTA CDS RGG TMT CTA ATC-3', 5'-TAC NVG GGT ATC TAA TCC-3') reverse primers. A and B adapters were added to primers according to the equipment manufacturer's manual (Roche, USA). The forward primer used for each sample received an 8-base ID tag to identify the attributed sequences. The samples were pyrosequenced abroad by a third party (Macrogen Inc., Seoul, South Korea). 
library_name: Bulk soil 1