title: Illumina MiSeq paired end sequencing; RNA-Seq of Acinetobacter baumannii ATCC 17978 and a gene knockout strain of putative response regulator gene A1S_2006 from the ATCC 17978 background
design: RNA-Seq of Acinetobacter baumannii ATCC 17978 and a gene knockout strain of putative response regulator gene A1S_2006 from the ATCC 17978 background
library_name: Knockout strain 1 #1_p
library_construction_protocol: All cultures were grown in LB media at 37C with 250rpm shaking prior to extracting RNA samples for sequencing Total RNA was extracted from the cultures in their early log phase (O.D.600 = 0.6-0.8) using Qiagen RNeasy mini kit as per manufacturer's instructions Library construction was performed according to the required specifications by Illumina MiSeq platform
attributes.tag: Experimental Factor: genotype
attributes.value: A1S_2006 gene knockout