title: TCRseq of mus musculus: Recipient and Donor T cells; Gut; Spleen
design: 5RACE (Rapid amplification of cDNA ends) were used to capture the 5end of TRA and TRB using a universal primer and TRAC primer(5>NNNNNNTACACGGCAGGGTCAGGGTTCTGGATA) and TRBC primer(5>NNNNNNGGCTCAAACACAGCGACCTCGGGTGGGAACAC) which were designed against each constant region of TRA and TRB. KAPA Hyper Prep Kit (KAPA Biosystems KK8504) were used for library-prep of PCR products following the instructions. 300bp or 250bp  2 paired-end FASTQ data were obtained from Miseq sequencer platform (Illumina).
library_name: T4-D18-MAJ-BR-GI-03