title: Targeted Deep Sequencing of CRISPR/Cas off-target sites for sgRNA targeting CCR5 in Human HSPC's
design: Identified off-target sites for sgRNA targeting CCR5 using in silico tool COSMID. Sites were amplified and subjected to NGS to determine the off-target cutting frequencies under different conditions.
library_name: Cord blood-derived HSPC-2 with WT Cas9