title: high-throughput small RNA sequencing of upland cotton root
design: A total amount of 1.5 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra small RNA Sample Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. PAGE gel was used to electrophoresis fragment screening purposes, rubber cutting recycling as the pieces get small RNA libraries. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumia) according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500 platform and paired-end reads were generated
library_name: Cotton_A292-S07_1