title: GSM2695471: LEF1cKO_Rep1; Mus musculus; RNA-Seq
library_construction_protocol: Activated hair gem stem cells (by hair depilation) were purified from wild-type, LEF1 single knock out, and LEF1/TCF7 double knock out mice. FACS-isolated cells were directly lysed into TRIzol LS (Invitrogen). Total RNA was purified using the Direct-zol RNA MiniPrep kit (Zymo Research) per manufacturer’s instructions. Quality of the RNA for sequencing was determined using an Agilent 2100 Bioanalyzer; all samples used had RNA integrity numbers >8. Library preparation using the Illumina TrueSeq mRNA sample preparation kit was performed at the Weill Cornell Medical College Genomic Core facility (New York), and RNAs were paired-end sequenced on Illumina HiSeq 4000 machines.
attributes.tag: GEO Accession
attributes.value: GSM2695471