title: "RAD-seq of kiwifruit, F1 progeny S44"
design: "Briefly, genomic DNA from each of 18 progenies were digested for 15 min at 37oC in a 50 uL reaction with 20 units (U) of EcoR I (NEB, USA). P1 adapter, a modified Illumina adapter, was ligated to the samples. After adapter ligation, sample were pooled and randomly sheared with a Bioruptor (Diagenode, Belgium) into an average size of 500 bp. DNA fragments in the range of 300-500 bp were purified using the MinElute Gel Extraction Kit (Qiagen). The dsDNA ends were repaired using the Quick Blunting kit Enzyme Mix (NEB). Then, a modified Solexa P2 adapter was ligated to the obtained DNA fragments. Finally, purified and quantified DNA products were PCR-amplified using Phusion Master Mix (NEB, USA). The prepared DNA libraries were sequenced on the Illumina Hiseq2000 instrumentation."
library_name: F13FTSCCKF2054 - F1 progeny S44