title: Illumina HiSeq 3000 paired end sequencing; Pig histone modification atlas
design: ChIP-Seq 100 cycle paired end experiment on an Illumina HiSeq 3000
library_name: 27AC-P7LPS2H
library_construction_protocol: NEBNext Ultra II DNA Library prep kit for Illumina
attributes.tag: project
attributes.value: FAANG
attributes.tag: assay type
attributes.value: ChIP-seq
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation, other
attributes.tag: sampling to preparation interval
attributes.value: 1
attributes.tag: experimental protocol
attributes.value: https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7645.pdf
attributes.tag: extraction protocol
attributes.value: http://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_ChIP_swine_immune_cells_20190124.pdf
attributes.tag: library preparation location
attributes.value: DNA facility, Iowa State University
attributes.tag: library preparation date
attributes.value: 2019-05-08
attributes.tag: sequencing location
attributes.value: DNA facility, Iowa State University
attributes.tag: sequencing date
attributes.value: 2019-05-10
attributes.tag: experiment target
attributes.value: histone_modification
attributes.tag: chip protocol
attributes.value: http://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_ChIP_swine_immune_cells_20190124.pdf
attributes.tag: control experiment
attributes.value: ISU-USDA-FAANG-AlvMac-ChIPseq-C7LPS2H-input
attributes.tag: chip target
attributes.value: H3K27Ac
attributes.tag: chip antibody provider
attributes.value: Diagenode
attributes.tag: chip antibody catalog
attributes.value: H3K27ac (Diagenode, catalog: C15410196) H3K4me3 (Diagenode, catalog: C15410003)
attributes.tag: chip antibody lot
attributes.value: A1723-0041D
attributes.tag: library generation max fragment size range
attributes.value: 600
attributes.tag: library generation min fragment size range
attributes.value: 50