title: GSM4511874: IRF8KO_LMPP_input_DNA; Mus musculus; ChIP-Seq
library_construction_protocol: Sorted cells were fixed with 1% formaldehyde for 10 min at room temperature and the reaction was quenched with 125 mM glycine. The lysates were sonicated using a Diagenode Bioruptor-Pico sonication device (12 cycles of 30 seconds on and 30 seconds off). An aliquot of sheared chromatin was immunoprecipitated with anti-H3K27ac antibody (MABI0309, Cosmo Bio) using Dynabeads M-280 sheep anti-mouse immunoglobulin G (IgG) (Thermo Fisher Scientific) or anti-IRF8 antibody (C-19, Santa Cruz) using Dynabeads protein G (Thermo Fisher Scientific) at 4˚C. Then, ChIP-seq libraries were prepared with a TruSeq ChIP sample prep Kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4511874