title: RNA-Seq of tumors from cord blood-humanized mouse model
design: RNA samples were harvested from frozen primary tumor tissue by homogenizing tissue with the Covaris Cryoprep Pulverizer. The pulverized tissue was then lysed in Trizol reagent (Invitrogen). RNA was isolated using phenol/chloroform extraction and total RNA was quantitated using a NanoDrop 2000 Spectrophotometer (ThermoFisher). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Prep kit (Illumina).
library_name: P3HR1_1