title: Whole exome sequencing of mus musculus: mTSG brain 16
design: The mouse whole-exome capture was performed using SeqCap EZ exome kit (Roche). Briefly, capture sequencing was done following standard Illumina-Roche-Illumina protocols. Genomic DNA samples from mouse organs were subjected to fragmentation, followed by a library preparation step using KAPA Library Preparation Kit (Illumina). The libraries were then amplified using LM-PCR, hybridized to the exome probe pool, washed and recovered, and amplified with multiplexing barcodes using LM-PCR. The multiplexed library was then QCed using qPCR, and subjected to high-throughput sequencing using the Hiseq-2500 or Hiseq-4000 platforms (Illumina) at Yale Center for Genome Analysis.
library_name: M031SR42_exome