title: GSM3639239: BC3; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA from untreated and SQ0048strain-treated BVECs was extracted using Trizol reagent (Invitrogen/Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer's instructions. The quantity and purity of total RNA were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA), yielding RIN scores >7.0. The cDNA libraries were constructed with RNA isolated from BVECs using a TruSeq Stranded mRNA library preparation kit (Illumina, San Diego, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3639239