title: Metagenomics Analysis of Chandeleur Island saltmarsh 2015
design: Shotgun DNA sequencing was performed at the Genomic Services Laboratory at Hudson Alpha located in Huntsville, AL. Genomic DNA extractions from five sediment cores per condition (vegetated, subtidal) within a given month (July – September) were used for sequencing. In total, 1 μg from each vegetated gDNA sample (n=15) and subtidal gDNA sample (n=15) were submitted in duplicate for high throughput sequencing.  Sequencing libraries were prepared with an Illumina Nextera DNA Library KitTM, barcoded, and sequenced using Illumina HiSeq v4 technology.  Samples were pooled and run using two flow cell lanes.
library_name: JSubC4