title: GSM3335673: CELF6 CLIP [yfp-4-ip]; Mus musculus; RIP-Seq
library_construction_protocol: On post-natal day 9, mice were rapidly decapitated and brains were removed, with cortices/cerebella discarded. Remaining tissue was pooled per litter among YFP and WT samples and snap frozen according to method above in “summary” After immunoprecipitation, samples were separated by SDS-PAGE and transferred to PVDF. A region 80-150 kDa (MW Celf6 HA-YFP ~78kDa) was cut and RNA was liberated with Proteinase K digestion, and extracted with acid phenol/chloroform, and purified and treated with DNase on a Zymo-5 RNA Clean and Concentrator column. Follows Van Nostrand et. al. eCLIP method (Nat. Methods 2016) with some oligonucleotide sequence modifications for adapter ligation and library PCR with index (“read 3”) primers for sample demultiplexing
attributes.tag: GEO Accession
attributes.value: GSM3335673