title: Illumina HiSeq 2500 paired end sequencing
design: no description
library_name: no name
library_construction_protocol: Aliquots of 1�g DNA per sample were used to create sequencing libraries. First, genomic DNA was fragmented using a Covaris S2 sonicator (Covaris Inc., Woburn, USA) to approximately 300bp. Fragmented DNA was quantified and used to synthesize shotgun libraries with the NebNext Ultra DNA library preparation kit for Illumina (New England Biolabs, Ipswich, USA), according to manufacturer�s instructions. PCR cycling conditions were set to a minimum of 4 cycles for annealing/extension to minimize PCR duplicates. NEBNext Singleplex Oligos for Illumina were used for indexing samples without multiplexing.