title: Illumina HiSeq 1500 sequencing; RNAseq analysis of early touch responses in myc234 mutants in Arabidopsis
design: RNAseq analysis of early touch responses in myc234 mutants in Arabidopsis
library_name: Col-0 25 min touch rep 1_s
library_construction_protocol: Samples were quickly collected from the petri dishes with fine tweezers, dried with tissue paper, placed into an eppendorf tube and immediately snap frozen Plants were grown in vitro for two weeks on petri dishes containing MS media with 2% sucrose. Plants were grown under long day conditions with approx. 100 micromol photons per second per square meter Plants were sprayed with water using a standard hairspray bottle (mister) RNA was prepared using the Sigma Plant Spectrum Total kit, with on-column DNA digestion and Ambion DNA-free kit Libraries were contructed using the Illumina RiboZero Plant kit
attributes.tag: Experimental Factor: stimulus
attributes.value: mechanical stimulus - water from spray bottle
attributes.tag: Experimental Factor: time
attributes.value: 25
attributes.tag: Experimental Factor: genotype
attributes.value: wild type genotype