title: GSM3445966: F1-LPD-rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from iWAT using TRIZOL reagent (Thermo Fisher Scientific, Waltham, MA). RNA quality was evaluated using an RNA 6000 Pico kit (Agilent, Santa Clara, CA). Fifty nanograms of total RNA was used for RNA sequencing (RNA-seq) library preparation with a NEBNext rRNA Depletion Kit and a NEBNext Ultra Directional RNA Library Preparation Kit (New England Biolabs, Ipswich, MA), then 2 × 36 base pair-end sequencing was performed using NextSeq500 (Illumina, San Diego, CA) by Tsukuba i-Laboratory LLP (Tsukuba, Ibaraki, Japan). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3445966