title: GSM3333103: P15 PDGFRa-GFP+ cells; Mus musculus; RNA-Seq
library_construction_protocol: Pdgfra-GFP mouse lungs at P7 and P15 were sacrificed and each lung lobes were dissected off and minced into small pieces in a conical tube containing 6ml of 2U/ml dispase (STEMCELL Technologies) followed by rotating incubation for 30 min at 37°C. The cells were placed on ice and added 15ul 10mg/ml DNase I (Sigma) for 5 min. The cells were then filtered through 40um strainers and centrifuged at 800rpm for 6 min at 4°C. The cell pellet was resuspended in 1ml of RBC lysis buffer (Abcam) and lysed for 90 s at room temperature. Addition of 6ml DMEM media (GIBCO) was followed and 500 mL of FBS (Hyclone) was slowly added in the bottom of tube. Cells were centrifuged at 800rpm for 6 min at 4°C. The cell pellet was resuspended in PBS with 3% BSA (GIBCO) for flow cytometry, 4', 6-diamidino-2-phenylindole (DAPI) (Sigma) was used to eliminate dead cells. Cell sorting was performed with a FACS Aria II. 10X Genomics
attributes.tag: GEO Accession
attributes.value: GSM3333103