title: RNA-seq of V. dahliae strain JR2: mycelium
design: RNA extraction was carried out using TRIzol following manufacturer guidelines. Following RNA re-suspension, contaminating DNA was removed using the TURBO DNA-free kit mRNA were enriched based on polyadenylation purification and random hexamers were used for cDNA synthesis. RNA-sequencing libraries were constructed following end-repair and adapter ligation protocols and PCR amplified.
library_name: JR2-RNA-PDA-2