title: GSM2938898: ChIP-seq for ZNF701 [SL116164]; Homo sapiens; ChIP-Seq
library_construction_protocol: Exponentially growing cells from ENCODE Tier 1 and Tier 2 cell lines were crosslinked in 1% formaldehyde and harvested in batches of 20 million cells per replicate.  Prepared nuclei were resuspended in RIPA buffer and chromatin shearing was performed on a Bioruptor Twin instrument (Diagenode).   Antibody (5 µg/rxn) was coupled to M-280 Dynabeads (ThermoFisher Scientific; 11201) overnight prior to addition of sheared chromatin.  A small aliquot of the sheared chromatin was reserved as an input control.  Following incubation and wash steps, captured chromatin and input control were reverse crosslinked overnight at 65°C and recovered using the QIAquick PCR Purification System (Qiagen; 28104). ChIP-seq libraries (illumina) were prepared and run on an Illumina HiSeq 2500 instrument.
attributes.tag: GEO Accession
attributes.value: GSM2938898