title: Illumina HiSeq 3000 paired end sequencing; Bovine gene expression atlas
design: Paired end RNA sequencing of bovine tissues using polyA selection on an Illumina HiSeq3000
library_name: BTA-DEDJTR-6819-Fsub-RNA
library_construction_protocol: SureSelect Strand Specific RNA Library Prep Kit
attributes.tag: assay type
attributes.value: RNA-seq of coding RNA
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 20
attributes.tag: extraction protocol
attributes.value: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/Trizol_Plus_man.pdf
attributes.tag: library preparation location
attributes.value: Department of Economic Development, Jobs, Transport and Resources
attributes.tag: library preparation location longitude
attributes.value: 144.96332
attributes.tag: library preparation location latitude
attributes.value: -37.814
attributes.tag: library preparation date
attributes.value: 2016-10
attributes.tag: sequencing location
attributes.value: Department of Economic Development, Jobs, Transport and Resources
attributes.tag: sequencing location longitude
attributes.value: 144.96332
attributes.tag: sequencing location latitude
attributes.value: -37.814
attributes.tag: sequencing date
attributes.value: 2016-11
attributes.tag: experiment target
attributes.value: CHEBI_33697
attributes.tag: rna preparation 3' adapter ligation protocol
attributes.value: https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
attributes.tag: rna preparation 5' adapter ligation protocol
attributes.value: https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
attributes.tag: library generation protocol
attributes.value: https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
attributes.tag: read strand
attributes.value: mate 1 sense
attributes.tag: rna purity - 260:280 ratio
attributes.value: 1.97
attributes.tag: rna purity - 260:230 ratio
attributes.value: 1.8
attributes.tag: rna integrity number
attributes.value: 6.1