title: Targeted sequencing of cancer cell line dilutions: MOLM13 diluted into SW48 (0.2%) - Rep 1
design: Illumina-compatible NGS libraries were prepared from each dilution point from cell line genomic DNA. Briefly, 100 ng DNA was sheared before library construction using a Covaris M220 sonicator (Covaris, Woburn, MA, USA) for 250 bp fragments in SmallDeep and LargeMid, respectively. The DNA libraries were constructed using the KAPA Hyper Prep kits (#KK8504, Kapa Biosystems, Wilmington, MA, USA) with custom UMI-containing adapters (Supplemental Table S3). Following end repair and A-tailing, adapter ligation was performed overnight using 100-fold molar excess of adapters. Agencourt AMPure XP beads (Beckman-Coulter) were used for library clean up and ligated fragments were amplified between 8 cycles using 0.5 uM Illuminal universal and sample specific index primers.
library_name: EPIC_059_L006