title: GSM4018901: MP-732-16; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from snap-frozen quadriceps muscles (n=4/sex/genotype) using RNeasy mini kit (Qiagen). The quality of total RNA was validated by Bioanalyzer (Agilent Technologies). Approximately 1 µg of RNA per sample was used to construct the cDNA library RNA libraries were prepared for sequencing using standard Illumina protocols, using the TruSeq stranded mRNA library prep kit (Illumina). The cDNA libraries were quantitated using KAPA library quantification kit for Illumina platforms (Kapa Biosystems). The individual indexed libraries were diluted to 4 nM and pooled in equal quantity and denatured before loading onto the Illumina NextSeq 500. The sequencing was run as paired-end reads (2 × 75 bp per read) with a targeted depth of 60 million paired-end reads per sample.