title: RNA-seq of Nicotiana tabacum: SgRVE6-OE3 transgenic tobacco leaf replicate2
design: Total RNA was isolated from the third leaves of tobacco plants using RNAprep Pure Plant Kit (TianGen Biotech, China). The RNA was electrophoresed on 1% agarose gels for monitoring RNA degradation and contamination. RNA purity, concentration, and integrity were further assessed using Qubit2.0 (Thermo Scientific, USA) and Agilent 2100 (Agilent Technologies, CA, USA). RNA-Seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), following manufacturer's instructions. RNA-seq libraries were sequenced using Illumina HiSeq X-ten platform.
library_name: OE3_2