title: nifH targeted-metagenomics of Frankia in soil
design: This approach was based on 606bp amplicons generated using primers nifHf1 and nifHr as the template in a nested PCR reaction using modified primers nifHf1 and nifH269 to create a 263 bp product. In the nested reaction, primer nifH269 was barcoded, and both primers were modified with Illumina adapters, primer pad, and linkers
library_name: Peru.4A