title: Genome Resequencing of O. tshawytscha: pooled tissue
design: DNA was normalized using picogreen fluorescence; fragmented with the NEBNext Ultra dsDNA Fragementase, pooled and filtered with Minelute purification. The NEBNext Ultra End Prep was used for fragment end repair and fragments between 400-500bp were selected using 25x AMPure beads. Fragments were amplified with NEBNext Ultra Q5 PCR, cleaned with AMPure beads, and quantified.
library_name: L0636