title: U1 DNAseq
design: Urine pooled samples were digested with proteinase K and processed with the DNeasy Blood/Tissue kit (Qiagen) to extract the corresponding DNA material. DNAs were amplified by a non-bias random-primer protocol ((Cantalupo et al. mBio. 2011;2(5). Epub 2011/10/06. doi: 10.1128/mBio.00180-11. PubMed PMID: 21972239; PubMed Central PMCID: PMCPMC3187576) and the resulting samples were submitted to the Genomics Research Core of the University of Pittsburgh (https://www.genetics.pitt.edu/) for library construction and Illumina sequencing.
library_name: U1_DNA