title: GSM3500733: NRCM_hypoxia_4n_rep1; Rattus rattus; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Micro Kit (Cat#74004, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set  (Cat#79254, QIAGEN, GmBH, Germany). According to the experimental instructions, the purified total RNA was subjected to mRNA isolation, fragmentation, first strand cDNA synthesis, second strand cDNA synthesis, terminal repair, 3' end plus A, ligation linker, enrichment and other steps to complete the library construction of the samples.
attributes.tag: GEO Accession
attributes.value: GSM3500733