title: GSM4453811: RT4_HiC; Homo sapiens; Hi-C
library_construction_protocol: Hi-C was performed using the Arima-HiC kit as per the manufacturer's protocol. Briefly, 1 million fresh and detached cells or 20mg tumor tissue (frozen and pulverized) was cross-linked with 1% formaldehyde, restriction enzyme digested, Biotin-14-dATP end-labeled , and subsequently ligated.  Ligated chromatin product was then reverse-crosslinked, sonicated (Covaris E220) to 300–500 bp fragments, pulled down with dynabeads Streptavidin  C1 beads. Hi-C DNA was then processed using Kapa Hyper Prep Kit where it was end repaired, adenylated, adaptor ligated, and PCR amplified with KAPA DNA library prepare kit. Pair-end 2x50/2x100/2x150 bp high-throughput sequencing was performed using HiSeq/NovaSeq platform (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4453811