title: GSM4199203: prg-1(n4357) Mutant Dissected Goands sRNA 20C, Rep 1; Caenorhabditis elegans; miRNA-Seq
library_construction_protocol: sample collection: Dissected gonads were collected in Trizol on ice over the course of 8-10 hours, then stored at -80 C. The Trizol containing the gonad tissue underwent 2 freeze/thaw cycles using liquid nitrogen prior to Trizol/chloroform extraction. Small RNAs (~16-30-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase to facilitate capture of 22G-RNAs. Libraries were then prepared according to the NEBNext® Multiplex Small RNA kit protocol. PCR products (~134-148 bp) were size selected using 10% polyacrylamide gels.
attributes.tag: GEO Accession
attributes.value: GSM4199203