title: 16S amplicon of poultry litter
design: The 16S rRNA gene V1V3 region was amplified using previously published primers fused to barcodes and adaptor sequences for the MiSeq instrument.  Briefly, PCRs contained the following: 17 l AccuPrime Pfx SuperMix (Life Technologies, Grand Island, NY), 5.0 M each of the primers i5+V3 and i7+V1 , and 25 ng of DNA. The following PCR conditions were used: 2 min at 95 C, 22 cycles of [20 s at 95 C, 15 s at 55 C, 5 min 72 C], 72 C for 10 min.
library_name: S388