title: GSM2696362: IL2RGA_rep3_bulkRNA; Danio rerio; RNA-Seq
library_construction_protocol: Wild-type and mutant zebrafish kidney from individual animals were surgically dissected and placed into 350 µl of QIAGEN RLT buffer containing 1% 2-Mercaptoethanol, followed by RNA isolation using the RNAeasy Mini kit (QIAGEN). 100 ng of RNA isolated from each animal was subjected to mRNA selection by the RNA NEBNext® Poly(A) mRNA Magnetic Isolation Module, followed by NGS library construction using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina using 15 cycles of PCR amplification. Libraries were sequenced on the Illumina HiSeq 2000 platform by the High Output Paired End 50bp method with 1% PhiX spike-in.
attributes.tag: GEO Accession
attributes.value: GSM2696362