title: GSM2935586: WT_1; Mus musculus; RNA-Seq
library_construction_protocol: Total brain (cortex) RNA extracts were purified according to manufacturer's instruction using the Qiagen Qiazol Lysis Reagent (Cat# 79306) and RNeasy Mini kit (Cat# 73404). Paired-end sequencing libraries were prepared by the Mayo Clinic sequencing core facilities (Rochester, MN) with the TruSeq Stranded Total Sample Preparation kit (Illumina), after which samples were subjected to quality control, cluster generation, and sequencing at a read depth of 60 million reads per sample on the Illumina HiSeq 2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM2935586