title: Illumina HiSeq 2000 paired end sequencing; Acquired TET2 mutation-linked clonal hematopoiesis in FPD/AML patient led to the development of pre-leukemic clone resulting independently in T2-ALL and AML-M0.
design: Acquired TET2 mutation-linked clonal hematopoiesis in FPD/AML patient led to the development of pre-leukemic clone resulting independently in T2-ALL and AML-M0.
library_name: SC_Fib
library_construction_protocol: cutaneous biopsy was performed and fibroblasts were culture in MEM medium supplementend with 10%FBS; penicilline, streptomycine and Fungizone. DNA was extracted using Qiagen Kit The fragment ends were repaired, “A” bases were added ends of the DNA fragments and adapters were ligated to the fragments. The adapter-ligated library was amplified using 4 to 6 cycles of PCR. 500 nanograms of the sample library were hybridized with the SureSelect biotinylated RNA library for 24 hours. Bound DNA was purified using streptavidin coated magnetic beads
attributes.tag: Experimental Factor: thrombopenia
attributes.value: disease staging