title: Illumina HiSeq 2000 paired end sequencing; Transcriptome analysis in the Antarctic bacterium Pseudomonas extremaustralis at low temperatures
design: Transcriptome analysis in the Antarctic bacterium Pseudomonas extremaustralis at low temperatures
library_name: 30_1.f
library_construction_protocol: Bacterial cells were collected by centrifuation for 10 min at 5000xG Aerobic cultures perfomed at 30 degree C in LB with 0.25 % sodium octanoate until reached OD600 of 0.5 Performed with ScriptSeq v2 RNA seq library prep kit (Illumina) acoording to manufacture instructions tRNA depletion with Microbe express kit (ambion) adding also 5S oligonucleotides RNA straction usign TRIZOL method Performed with ScriptSeq v2 RNA seq library prep kit (Illumina) acoording to manufacture instructions
attributes.tag: Experimental Factor: temperature
attributes.value: 30 degree Celsius