title: GSM1154518: Rrp5NTD; Saccharomyces cerevisiae BY4741; RNA-Seq
library_construction_protocol: Lysates were clarified and the histidine-tagged proteins were purified on nickel columns (Qiagen Ni-NTA agarose). A two-step IP was performed with one in denaturing condition (6M guanidinium). RNA-Rrp5 complexes were separated by SDS-PAGE, transferred to a nitrocelluose membrane and extracted. Protein was removed by Proteinase K digestion and RNAs purified by phenol:chloroform extraction. Extracted RNAs were converted to cDNA and PCR amplified. PCR products between ~150 and 250 bp were gel extracted and submitted for Solexa sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1154518