title: GSM4143065: NRDE3_IP_small_RNA_ GFPNRDE3; Caenorhabditis elegans; ncRNA-Seq
library_construction_protocol: Total small RNAs were isolated from embryo stage worms. Briefly, worms were harvested and dounced on ice with pestle B in TRIzol reagent (Invitrogen) followed by isopropanol precipitation. The precipitates were treated with calf intestinal alkaline phosphatase (CIAP, Invitrogen), re-extracted with TRIzol, and treated with T4 polynucleotide kinase (T4 PNK, New England Biolabs) in the presence of 1 mM ATP. Small RNAs were cloned and deep sequenced using an Illumina platform, according to the manufacturer's instructions, by  Novogene (Beijing, China). sRNAs ranging from 17 to 30 nt were gel-purified and ligated to a 3' adaptor (5'-pUCGUAUGCCGUCUUCUGCUUGidT-3'; p, phosphate; idT, inverted deoxythymidine) and a 5' adaptor (5'-GUUCAGAGUUCUACAGUCCGACGAUC-3'). The ligation products were gel-purified, reverse transcribed, and amplified using Illumina's sRNA primer set (5'-CAAGCAGAAGACG GCATACGA-3'; 5'-AATGATACGGCGACCACCGA-3'). The samples were sequenced using an Illumina Hiseq platform.
attributes.tag: GEO Accession
attributes.value: GSM4143065