title: Bulk T cell receptor beta chain sequencing within HIV infected human lymph node: Sample S18
design: TCR beta chain mRNA were reverse transcribed using gene-specific primers ligated to a 12N molecular identifier and a sequencing adaptor. 1st PCR was carried out using the adaptor sequence and forward primers against the variable regions. 2nd PCR was performed to add the full illumina sequencing adaptors. 2nd PCR products were gel purified and quantified via Agilent Tapestation before pooling for sequencing.
library_name: H24_gc_tfh