title: GSM3446075: Hi-C of chicken immature erythocytes rep1; Gallus gallus; Hi-C
library_construction_protocol: Immature erythrocytes was extracted from blood of anemic adult (2-5 months old) female chickens. Briefly, we injected animals with 1ml of 5% phenylhydrazine (Sigma Aldrich, P26252) solution daily for 4 days. At day 5 after beginning of injections, blood was collected from phenylhydrazinetreated and control animals According to Brilliant Cresyl Blue staining, proportion of immature erythrocytes at this point was about 95% (189 out of 200 counted cells) in treated animals and 3.5% (9 out of 256 counted cells) in control animals. Blood was centrifuged at 500 g, 4°C for 10 min. Supernatant containing a layer of white cells was discarded, and red pellet was resuspended in 20 ml of cold PBS. The procedure of centrifugation and PBS-wash was repeated 2 more times. Finally, cells were counted, and desired amount of erythrocytes was pelleted and resuspended in fixation solution. For each sample (red blood cells and fibroblasts) two independent fixations (biological replicas) were performed. All Hi-C libraries were prepared using in situ protocol, as described in Rao S.S. at all Cell (PMID: 25497547). DNA libraries were sequenced from both ends on Illumina HiSeq 2500 platform in 3 runs total. The sequencing was conducted in 2x101 and 2x106 mode with TruSeq Rapid SBS chemistry, and in 2x126 mode using HiSeq® SBS Kit v4
attributes.tag: GEO Accession
attributes.value: GSM3446075