title: GSM4509292: MouseACS1; Mus musculus; RNA-Seq
library_construction_protocol: Post-natal day 19 (P19) Chx10-cre-GFP animals were euthanized by intraperitoneal injection of Euthasol. Eyes were removed and retinas dissected in oxygenated Ames solution between 10 and 11AM. Retinas were incubated at 37°C for 10 minutes in a papain solution followed by trituration in an ovomucoid solution to quench papain activity and generate a single cell suspension. Cells were centrifuged at 450 x g for 8 minutes and the pellet was resuspended in Ames+4% BSA with rat anti-mouse CD133-APC (eBioscience) and rat anti-mouse CD73-PE (eBioscience). Following incubation for 15 minutes at room temperature, cells were washed with Ames + BSA, centrifuged again, and resuspended at a concentration appropriate for flow cytometry. Samples were sorted on a MoFlo Astrios cell sorter (Beckman) and cells triple negative for GFP, APC, and PE but positive for the cell viability marker Calcein Blue were collected. Single cell libraries were made with Chromium 3' v2 platform (10X Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, single cells were partitioned into Gel bead in EMulsion (GEMs) in the GemCode instrument with cell lysis and barcoded reverse transcription of RNA, followed by amplification, shearing and 5' adaptor and sample index attachment. On average, approximately 10,000 single cells were loaded on each channel and approximately 6,000 cells were recovered. Libraries were sequenced on Illumina HiSeq 2500. Chromium 3' v2 platform (10X Genomics, Pleasanton, CA) 
attributes.tag: GEO Accession
attributes.value: GSM4509292