title: GSM3399555: MCF10A_NT_rep3; Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA was extracted using the mirVana miRNA isolation kit Transfer RNA profiling was done as described by (Goodarzi et al., 2016). Briefly, biotinylated probe-pairs against nuclear encoded tRNAs were designed to hybridize to each half of the tRNA. A nick at the anticodon end of the DNA-RNA hybrid was filled using T4 DNA ligase and SplintR ligase (NEB). MyOne-C1 Streptavidin Dynabeads (Invitrogen) were used to purify the DNA-RNA hybrids and ligated probes were eluted following RNase H and RNase A incubation. Eluted probes were PCR amplified and high-throughput sequenced.
attributes.tag: GEO Accession
attributes.value: GSM3399555