title: GSM4509467: sdGAL4_Dam6; Drosophila melanogaster; OTHER
library_construction_protocol: Wing discs from female larvae were collected into cold PBS, genomic DNA was extracted using a Zymo Quick-DNA kit (#D4069). DNA was digested with DpnI, DamID adaptors ligated, digested with DpnII and amplified via PCR. DNA was sonicated to an average size of 300bp using Covaris ME200 sonicator and DamID adaptors removed via AlwI digestion.  Sonicated material was end-repaired, adenylated, sequencing adaptors were ligated and samples were PCR amplified.
attributes.tag: GEO Accession
attributes.value: GSM4509467