title: GSM4293417: wt treatment 1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Worms were removed and flash frozen in liquid nitrogen in using Trizol reagent. Ribosomal RNA was depleted from 2.5 ug of total RNA using Illumina Ribo-zero Gold kit (Illumina). Strand specific RNA-seq libraries were constructed using SMARTer Stranded RNA-Seq HT Kit (Clontech #634829) according to manufacturer's protocol Strand specific RNA-seq libraries were constructed using SMARTer Stranded RNA-Seq HT Kit (Clontech #634829) according to manufacturer's protocol Based on rRNA depleted input amount, 15 cycles of amplificatoin were performed to generate RNA seq libraries.
attributes.tag: GEO Accession
attributes.value: GSM4293417