title: miRNA seq of Homo sapiens: HeLa cell
design: Total RNAs of cells were extracted using the TRIzol reagent (Thermo Fisher) and chloroform, and the concentration was determined on NanoDrop (Thermo Fisher). 20~50 µg total RNAs ran on a 15% TBE-Urea gel, from which the 18~28 nt band was excised. Resolved RNAs were then subject to, sequentially, 3’-ligation, 5’-ligation and reverse-transcription into cDNAs. Then, cDNAs were amplified by 12 PCR cycles and the products within the correct size ranges were collected from 20% TBE gels for quality tests (Fragment Analyzer, Agilent Technologies) and sequencing (Illumina HiSeq-2500 sequencer; run type: single-end; read length: 50 nt).
library_name: hela_wt_srna