title: 16S rRNA gene amplicon data of a filtered compost soil sample (2.7 µm)
design: 100 g compost soil of the Experimental Botanical Garden Göttingen, Germany (51°33‘22.6“N 9°57‘16.2“E, 180 m) were solved in 500 ml sterile H2O and filtered with a 2.7 µm filter. After filtering, genomic DNA was extracted using the PowerMax soil DNA isolation kit (MO BIO Laboratories). The 16S rRNA gene V3-V4 region was amplified using the primers S-D-Bacter-0341-b-S17 and S-D-Bact-0785-a-A-21 (Klindworth et al., 2013). The sequencing library was generated with the Nextera XT DNA library prep kit (Illumina). For sequencing, the MiSeq reagent kit version 3 (Illumina) was used.
library_name: REGE_a_104