title: Illumina Miseq of soil biocrust: Huelves dark
design: Soil bocrust were collected with petri dishes and stored dessicated in dark. After microscopic inspection half of the selected biocrust were composite  and the DNA from 300 mg of soil was extracted with MoBio soil DNA ilolation kit. DNA from three independent extractions was mixed an PCR was made with specific primers to cyanobacteria 359F and 781Ra and Rb in two idependent PCR reactions. Amplicons were processed using MiSeq sequencer (Illumina) read length 2X300 bp at a depth of 100,000 sequences per sample.
library_name: Hu.2