title: RNA-seq of Heterorhabditis bacteriophora: infective juvenile (IJ) soaked in Manduca sexta hemolymph for 9 hours
design: RNA samples were analyzed for quantity and quality using an Agilent 2100 Bioanalyzer with the RNA 6000 Nano Kit. Samples with a RIN greater than 9.0 were sent to the Institute for Genome Sciences (University of Maryland School of Medicine) for RNA-Seq using the Illumina HiSeq4000 platform with 150 bp, paired-end sequencing. RNA-seq libraries were prepared using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA). Double-stranded cDNA was ligated to seven indexed nucleotide adapters and purified between enzymatic reactions. Library size selection was performed using SPRIselect beads (Beckman Coulter Genomics, Danvers, MA).
library_name: XJOVA_20151015_K00134_IL100062657_S28