title: GSM3326071: RBMX_RIP_INPUT_Rep2; Homo sapiens; RIP-Seq
library_construction_protocol: Cell extracts were resuspended in NT2 buffer [50mM Tris HCl pH=7.5, 150mM NaCl, 1mM MgCl2, 0.05% NP40, 1 U/ml Ribolock (Fermentas, Glen Burnie, MD, USA), 2mM DTT, 30mM EDTA supplemented with a protease inhibitor cocktail (P8340, Sigma, RNA libraries were prepared for sequencing using standard Illumina protocols: the Illumina TruSeq Targeted RNA Custom Panel Kit was used with 500 ng of total or polysomal RNA.
attributes.tag: GEO Accession
attributes.value: GSM3326071