title: GSM3400707: D316A-Tg HFD [WAT-Activating_m8_s20]; Mus musculus; RNA-Seq
library_construction_protocol: Animals were euthanized under Schedule 1 of the Animals (Scientific Procedures) Act 1986. Subcutaneous white adipose was retrieved from the inguinal region near the flank, the lymph node removed and the tissue flash-frozen in liquid nitrogen. RNA extraction was performed using Trizol Reagent and isolated using Qiagen RNeasy Mini Kits as per manufacturers instructions. Total RNA quantification was performed using a NanoDrop spectrophotometer. RNA integrity was assessed, with a minimum RIN of 7 permitted for sequencing (Maryam Clausen, contributer). Illumina TruSeq stranded mRNA-seq protocol
attributes.tag: GEO Accession
attributes.value: GSM3400707