title: 16srDNA-seq of cecal content: control mice on Quinoa-based diet
design: Total bacterial DNA was isolated from frozen cecal contents using a QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA). The region V3-V4 of the 16S rRNA gene was amplified and DNA libraries were constructed according to previously described . The paired-end sequencing with a read length of 2250 bp was performed on the Illumina MiSeq platform.
library_name: QC