title: GSM2858457: EE2_1μM_3; Gadus morhua; RNA-Seq
library_construction_protocol: Total RNA was isolated from frozen slices using mirVana™ miRNA Isolation Kit (Cat# AM1560, Ambion, Austin, TX, USA). The RNA was treated using TURBO DNase (TURBO DNA-free kit, Ambion) and further purified using RNA Clean & Concentrator-5 (Zymo Research Corp, Irvine, CA, USA) and eluted with RNase-free water. The concentration of total RNA was measured using NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).  RNA quality was assessed using the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA samples (0.4 μg total RNA per sample) were sequenced according to Illumina TruSeq® Stranded mRNA Sample Preparation Guide on Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM2858457