title: PacBio sequencing of the Drosophila melanogaster Schneider-2 cell line S2R+
design: Cells from ten confluent T75 flasks were scraped into a 15mL Falcon tube and centrifuged at 300 x g for 3 min. The pellet was washed and resuspended in 1X PBS containing RNAse A. DNA was extracted with the Qiagen Blood & Tissue kit. Purified S2R+ DNA was used to generate PacBio SMRTbell libraries using the Procedure & Checklist 20 kb Template Preparation using BluePippin Size Selection protocol. The resulting library was sequenced on 31 PacBio RS II SMRT cells with a movie time of 240 minutes per SMRT cell.
library_name: S2R+_PB_1