title: GSM4237770: Splenic NK cells, MycKO_GH5; Mus musculus; RNA-Seq
library_construction_protocol: Spleen single cell suspensions were prepared and stained with NK cell markers. CD3-NK1.1+DX5+ live cells were sorted using a FACSAria Cell Sorter (BD Biosciences). Sorted cells were lysed directly in RLT buffer from the RNeasy Micro kit (QIAGEN), and total RNA was extracted according to the manufacturer's instructions. The NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) was used for library preparation, which is dUTP-based stranded library.The library was indexed and amplified under PCR cycle number of 8. After library Bioanalyzer QC analysis and quantification, individually indexed and compatible libraries were proportionally pooled and sequenced using HiSeq 1000 (Illumina, San Diego, CA). Under the sequencing setting of single read 1x51 bp, about 25 million pass filter reads per sample were generated.
attributes.tag: GEO Accession
attributes.value: GSM4237770