title: GSM2857384: KO_DR_WA_rep1; Mus musculus; RNA-Seq
library_construction_protocol: mRNA was isolated from brain, skeletal muscle, liver and white adipose tissue from WTAL (n=2), WTDR (n=3), KOAL (n=3) and KODR (n=3) female mice at 15 months of age (12 months of 30% DR) using Tri-Reagent (Life Technologies, Paisley, UK) and Qiagen RNeasy Mini Kit (Sussex, UK), following manufacturer's protocols. RNA was re-suspended in ultra-pure water, RNA concentration and purity was determined by spectrophotometry (NanoDrop, Fisher Scientific). The extracted RNAs were prepared using a Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher Scientific Inc.) to perform the poly A selection followed by an Ion Total RNA-Seq Kit v2 to prepare the library from the poly A selected RNAs. The constructed libraries were sequenced on an Ion Proton™ System (Thermo Fisher Scientific Inc.) using P1 chip (version P1.1.17) according to the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM2857384