title: 16S amplicon sequencing: salt marsh soil DNA
design: DNA was extracted from 1 g of soil by the freeze grind method. PCR was conducted on the V4 region in a two step method. After the first step the products were bead-purified and used as a template for the second step. The second PCR step used barcoded primers. The products of the second PCR were checked on a gel for the expected band size, quantified by Pico Green, and combined in equimolar amounts. The library was gel-purified and sequenced.
library_name: 21.sites.2018.05_MD1