title: GSM4293860: duox-/-(T4); Danio rerio; RNA-Seq
library_construction_protocol: Total RNAs from 4-dpf duox-/- and WT zebrafish were extracted, and 3 g of RNA per sample was prepared for constructed a transcriptional library. Sequencing libraries were generated with an UltraTM RNA Library Prep Kit (NEB, USA) according to the manufacturer's instructions. Clustering of the index-coded samples was conducted with a cBot Cluster Generation System using a TruSeq PE Cluster Kit (Illumina, USA), per the instructions. After clustering, the library preparations were sequenced on an Illumina Hiseq 2000 platform. Perl scripts were used to remove the adapter for clean reads, calculating the Q20/Q30 duplicate data, and generating the raw reads.
attributes.tag: GEO Accession
attributes.value: GSM4293860