title: Liquid wild type (control-r2): WT-8, WT-9, WT-10
design: The complementary DNA (cDNA) libraries were prepared using the TruSeq RNA Library Preparation kit v2 (Illumina) according to the manufacturers instruction. Polyadenylate-containing mRNA was purified by oligo(dT) magnetic beads from 1.0 _g of total RNA sample and fragmented using divalent cations. The cleaved RNA fragments were used for the first-strand cDNA synthesis using reverse transcriptase and random primers, followed by second-strand cDNA synthesis using DNA polymerase I and ribonuclease H. After second-strand cDNA synthesis, fragments were treated with end-repair, A-base tailing, and adapter ligation consecutively. The samples were amplified by PCR to create the final cDNA library
library_name: L_WT