title: VR1020:PBMC after vaccination control 2
design: Total RNA was used for RNA-seq library preparation from individual baboon samples of PBMCs, spleen cells, and lymph node cells with three biological replicates and two technical replicates along with their corresponding controls for each of the eight vaccine strategies. RNA was extracted using GenElute Mammalian Total RNA Miniprep Kit (SigmaAldrich, St. Louis, MO) following the manufacturers instructions. Total RNA concentrations were quantified  via Qubit 3.0 Fluorometer and RNA HS Assay Kit (Thermo Fisher,Waltham, MA) and RNA quality was determined using the Agilent 2200 TapStation (Agilent, Santa Clara, CA).Library preparation was completed as previously described (Zhang et al., 2018b) and stranded mRNA-sequencing on the NovaSeq 6000 platform (Illumina) was fulfilled following standard protocols by the Texas Tech University Center for Biotechnology and Genomics (Lubbock, TX).
library_name: PBMC PC97