title: GSM2945053: Sample1_Corn; Ovis aries; MBD-Seq
library_construction_protocol: Genomic DNA was extracted from longissimus dorsi muscle samples, following a salt-chloroform extraction protocol. DNA concentration and quality were estimated by PicoGreen® (Thermo Fisher, Waltham, MA, USA) and by agarose gel electrophoresis. One μg of genomic DNA was sonicated to produce DNA fragments of about 350 bp. Methyl-binding domain (MBD) enrichment was performed using the MethylMinerTM Methylated DNA Enrichment Kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer's instructions. Construction of the sequencing library was performed using the TruSeq® Nano Library Preparation Kit (Illumina, San Diego, CA, USA). Libraries were quality checked, quantified on an Agilent 2200 TapeStation High Sensitivity D1000 and used for sequencing in two lanes of an Illumina Hi-Seq 2000 (San Diego, CA). 100-base paired-end reads were generated.
attributes.tag: GEO Accession
attributes.value: GSM2945053