title: GSM2940572: Hand>Bru3-rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was extracted from about 10, 1-week or 5-weeks old, entire or dissected (head, legs, wings and ovaries removed) adult flies, using TRIzol reagent (Invitrogen) following manufacturer's instructions. The following genotypes dedicated for TU-tagging cell - specific transcript isolation were used: Hand>LacZ;HA-UPRT, Hand>MblRNAi;HA-UPRT, Hand>Bru3;HA-UPRT and Hand>960CTG;HA-UPRT Library preparation was performed on about 50 ng of cardiac specific RNA from the previously described conditions, using the Ovation RNA-Seq Systems 1-16 for Model Organisms adapted to Drosophila (NuGEN, #0350-32) following manufacturer's instructions with few modifications. First-strand synthesis was performed with the integrated DNAse treatment (HL-dsDNAse, ArcticZymes) and cDNA was fragmented using Bioruptor sonication system (Diagenode) with 30 cycles of 30 seconds ON/OFF on low power. 15 PCR cycles for library amplification were performed for 50 ng of starting RNA and a size-selection bead purification step was done using RNAClean XP beads (Agencourt AMPure, Beckman Coulter).
attributes.tag: GEO Accession
attributes.value: GSM2940572