title: GSM3445511: NT-Input; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% formaldehyde for 10 min and nuclei were pelleted after sequential wash and resuspended in sonication buffer (0.1% SDS, 1mM EDTA, 10mM Tris (pH8.1)) and sonicated in a Covaris to shear the chromatin into 200-300 bp  fragments and 10% Chromatin was decrosslinked and used as Input DNA. ChIP-seq libraries were constructed using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). DNA sequence was performed by NextSeq system (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3445511