title: GSM3445711: 4-cell 3; Bos taurus; Bisulfite-Seq
library_construction_protocol: Individual gamtes and embryos were lysis in a single tubes to obtain DNA using protease. We conducted DNA extraction, MspI digestion, end-repair, dA-tailing, adaptor ligation, bisulfite conversion, and PCR amplification libraries in single tubes to minimize DNA loss. Multiplex libraries using whole genome bisulfite sequencing approach.
attributes.tag: GEO Accession
attributes.value: GSM3445711