title: RNA-Seq of Thraustochytriidae
design: According to the manufacturer s guidelines, mRNA selection, library preparation and sequencing were performed using an Illumina HiSeq 4000 system after total RNA was extracted using TRIzol (Life Technologies, Thermo Fisher Scientific Inc., USA). The quality and quantity of the mRNA samples were assessed using a NanoPhotometer (IMPLEN, USA), a Qubit 2.0 Flourometer (Life Technologies), and an Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, USA). The cDNA library was constructed from mRNA samples with high purity and high integrity, created using random hexamers. After modification and enrichment for attachment to the Illumina flowcell, the resulting cDNA library products were subject to sequencing using the Illumina HiSeq 2000 and the obtained data was translated into raw reads by base calling. Each step in experiment process influences data quality and quantity, and then directly affect bioinformatics analysis results. To get high realiable sequencing data, we carry out strict quality control in each experiment step.
library_name: Mn16