title: GSM2693930: HEK293_siFKBP25_KD; Homo sapiens; RNA-Seq
library_construction_protocol: Transfected cells were harvested by trypsinization, pelleted by centrifugation, and flash frozen in liquid nitrogen. mRNA was purified using the µMACs mRNA isolation kit (Miltenyl Biotec, Cat. No . 130-075-102) and analyzed on an Agilent 2100 Bioanalyzer using Agilent 6000 RNA Nano Kit (Agilent Technologies, Cat. No. 5067-1511). cDNA was generated using a Superscript Double-Stranded cDNA Synthesis kit (ThermoFisher, Cat. No. 11917-010) followed by preparation of 75bp paired-end libraries using the Paired-End Sample Prep Kit (Illumina, Version 1.1). RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM2693930