title: RNA-Seq of Cyclocarya paliurus leaves
design: The resulting mRNA was fragmented into short fragments and used to the first-strand cDNA synthesis with random hexamer-primers and M-MuLV Reverse Transcriptase. Subsequently, second strand cDNA was performed using DNA polymerase I and RNase H ,and purified by AMPure XP beads. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, adaptor was ligated to prepare for library, the library fragments were purified with AMPure XP system, and PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. The library quality was assessed using the Agilent Bioanalyzer 2100 system.  The libraries were then quantified and pooled. Paired-end sequencing of the library was performed on the HiSeq XTen sequencers (Illumina Inc., San Diego, CA, USA) using the paired-end technology in a single run.
library_name: leaf HZ-3