title: GSM3034235: DB; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Cells were lysed with lysis buffer from NE BioLabs magnetic mRNA isolation kit #S1550S mRNA was purified and fragmented (Ambion #AM8740), then reverse transcribed to cDNA (Invitrogen SuperScript III # 18080051). DNA was purified by Agencourt Ampure XP beads and quantified by Qubit. DNA was repaired by End-It DNA Repair Kit (Epicentre, #ER0720) then supplemented with Klenow fragment (NE BioLabs #M0212s). Adapters were ligated using the T4 DNA Ligase Quick Ligase Kit (Enzymatics L603-HC-L) and PCR enriched using Phusion DNA polymerase (NE BioLabs #F-531). Libraries were gel purified, selecting DNA from 200-500bp, and gel extracted (Qiagen Qiaquick #28704).
attributes.tag: GEO Accession
attributes.value: GSM3034235