title: Miseq of soil metagenome: grapevine rhizosphere soil
design: Low quality reads in raw Illumina fastq data sets were filtered by Qiime version 1.9.0 (http://qiime.org/) and the chimera sequences were filtered by the uchime method of Mothur version 1.31.2 (http://www.mothur.org/). A window with a length of 5 bp was moved through each sequence. Sequences were truncated at the end of the window when the average quality score was less than the threshold of 20 (99% accuracy rate). All clean reads were overlapped by software FLASH version 1.2.7 (Fast Length Adjustment of Short reads, http://ccb.jhu.edu/software/FLASH/). Reads were truncated at any sites containing more than six consecutive bases, and any reads containing one or more ambiguous base calls were discarded.
library_name: E05Z