title: GSM4200857: dek46-ref; Zea mays; RNA-Seq
library_construction_protocol: RNA was extracted with Trizol reagent (Tiangen), followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Maize 35 mitochondrial genes were PCR-amplified from WT and dek46-ref kernel cDNA.RT-PCR amplicons obtained from each template were mixed in an equimolar ratio and  sheared by sonication. Sequencing libraries were generated using a NEB Next Ultra DNA.
attributes.tag: GEO Accession
attributes.value: GSM4200857