title: ATAC-seq of V. dahliae strain JR2: mycelium
design: A total of 200,000 nuclei were  added to the transposition reaction (20uL of 2x Nextera reaction buffer, 0.5uL of Nextera Tn5 Transposase, 19.5 uL of nuclease-free H20) and the reaction was carried out for 5 minutes at 37_C. The reaction was halted and fragmented DNA purified using a MinuElute PCR purification kit and amplified in reaction buffer for a total of 9 cycles.
library_name: JR2-ATAC