title: Illumina Genome Analyzer IIx sequencing; FOXM1 ChIP-seq in an OE33 cell line
design: FOXM1 ChIP-seq in an OE33 cell line
library_name: FOXM1 input rep1
library_construction_protocol: OE33 cells were cultured in RPMI 1640 medium containing 2 mM glutamine and 10% Fetal Bovine Serum. After cross-link reversal and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturers instructions. ChIP was performed as described in Lee et al. 2006 using 3x107 cells. Immunoprecipitation was performed overnight at 4C with 3 g antibody (anti-FOXM1 or control IgG) and 30 l Dynabeads per experiment. Lee, T. I., Johnstone, S. E., and Young, R. A. (2006). Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc 1, 729748. doi:10.1038/nprot.2006.98.
attributes.tag: Experimental Factor: input DNA
attributes.value: immunoprecipitate