title: GSM4511773: Vehicle 2; Mus musculus; RNA-Seq
library_construction_protocol: The tumor was dissected from the hind limb and dissociated by shaking for 45 minutes at 37°C in collagenase Type IV (Gibco), dispase (Gibco), and trypsin (Gibco). Cell suspension was then strained through a 40 µm filter, washed in PBS, and plated for culture. After tumor dissociation, the cells were filtered through a 40 µM strainer. Red blood cells were lysed using ACK Lysing Buffer (Lonza) and washed with flow buffer made of HBSS (cat 13175-095, Gibco), 5 mM EDTA (E7899, Sigma-Aldrich), and 2.5% FBS (Gibco). Cells were washed twice more in 0.04% bovine serum albumin (BSA) in PBS, then resuspended at 1000 cells per  µL. Cell suspensions were loaded on the 10x Genomics Chromium Controller Single-Cell Instrument (10x Genomics) using the Chromium Single Cell 3' Reagent V3 Kit.
attributes.tag: GEO Accession
attributes.value: GSM4511773