title: 16S rRNA seq of Apis mellifera: adult gut
design: PCR reaction conduced using primers with adaptors: 16SF_V3 (5' - 3' direction)  CCTACGGGAGGCAGCAG; and 16S R_V4 (5' - 3' direction)  GGACTACHVGGGTWTCTAAT. The PCR products (c. 420-440 base pairs) were purified to generate the libraries. The products were pooled in equimolar concentrations (confirmed with Qubit and LabChip (PerkinElmer, Waltham, MA, USA) analysis) respectively. The PCR products were sequenced with a 250-base paired end run on an Illumina MiSeq platform (Illumina Inc.) with version 2 chemistry. Illumina PhiX Control v3 (FC-110-3001) was included as the sequencing control.
library_name: 5dH1_S28