title: GSM2859392: N2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy mini kit (Qiagen, Germany) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Briefly, total RNA samples were treated using the RiboMinus™ Eukaryote Kit (A10837-08; Qiagen, GmbH, Germany) to remove ribosomal RNA. Next, ribosome-depleted RNA was treated with RNase R (RNR07250; Epicentre, Wisconsin, USA). Strand-specific RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7530L, NEB, Ipswich, MA, USA) following the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM2859392