title: GSM3501910: High Fat Diet + 17[beta]-estradiol mouse 3; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized by CO2 asphyxiation, periprostatic fat was harvested and snap frozen in liquid nitrogen prior to storage at -80C. Total RNA was extracted from frozen tissue using trizol reagent and the RNAeasy Mini Kit (Qiagen). RNA concentration (NanoDrop) and quality (Bioanalyzer) were measured. Only samples with RNA Integrity Number (RIN) >7 were used for RNAseq. Sequencing libraries were constructed following the Illumina TrueSeq Stranded Total RNA Library preparation protocol with rRNA depletion
attributes.tag: GEO Accession
attributes.value: GSM3501910