title: microbial diversity of ornamental fish aquarium water sample D1
design: target_taxon: Bacteria  target_gene: 16S rRNA  target_subfragment: V3-V5  pcr_primers: 341F2:CCTACGGGNGGCWGCAG; 341F2:TCTACGGAAGGCTGCAG; 925R1:CCGTCAATTCNTTTRAGT 926R3:CCGTCAATTTCTTTGAGT 926R4: CCGTCTATTCCTTTGANT  pcr_cond: initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;30 cycles
library_name: VS-454-MBL:assay:D1.3_Bacteria