title: GSM3333332: TAPBPR_1st-round-pos-2ugmlDd_R1; synthetic construct; OTHER
library_construction_protocol: Plasmid DNA was harvested from unsorted and sorted cells.  The scoop loop region was PCR amplified in 2 steps; in the first, primer overhangs added sequences for annealing to the Illumina sequencing primers, and in the second, primer overhangs added unique barcodes and Illumina adaptors. Primer pair for 1st round of PCR: Illumina_TAPBPR_111F (GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAGACTGTTTCCTGGTGAAGG) and Illumina_TAPBPR_229R (TCTTTCCCTACACGACGCTCTTCCGATCTCCTTGGAAATCGGTGAAGTC) Primer pair for 2nd round of PCR (added I5 and I7 Illumina adaptors): MiSeq_Start_Adaptamer (AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT) and MiSeq_Index_Adaptamer (CAAGCAGAAGACGGCATACGAGAT-6nt-barcode-GTGACTGGAGTTCAGACGTGTGCTCTTC) Amplicons were deep sequenced on a HiSeq 4000 using a 2x150 nt paired end protocol.
attributes.tag: GEO Accession
attributes.value: GSM3333332