title: GSM2938715: ED3.4_defeated_BNST; Mus musculus; RNA-Seq
library_construction_protocol: Brain regions were dissected on a sterile chilled petri dish within 7 minutes and tissue was flash-frozen in liquid N2. Total RNA was extracted with TriReagent (Molecular Research Center, Inc.) and RNA integrity was controlled with Bioanalyzer (Agilent). The rRNA was depleted with either Ribo-Zero Gold rRNA Removal Kit (Illumina; mPFC and vHPC) or custom designed Insert dependent adaptor cleavage (InDA-C) primers (BNST). Sequencing libraries were prepared with Nextera (Illumina; vHPC), ScriptSeq v2 (Epicentre; mPFC), and Ovation Universal RNA-Seq System (NuGEN; BNST) RNA-seq library preparation kits.
attributes.tag: GEO Accession
attributes.value: GSM2938715