title: GSM4292944: S04_NSuSt; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq analysis was done in collaboration with the Centre for Health Genomics and Informatics at the University of Calgary. Naïve hPSCs from static, static suspension  and stirred suspension cultures were collected and the total RNA was extracted using PureLink™ RNA Mini Kit (Thermo Fisher Scientific, 12183018A), according to the manufacturers' protocol, including DNAse I digestion using DNAse I Amplification Grade (Thermo Fisher Scientific, 18068015). Concentration and quality of the total extracted RNA was checked using Qubit Fluorometer (QubitR RNA HS high sensitivity assay Thermo Fisher) and TapeStation assay (Agilent 4200 TapeStation System), respectively. The total RNA was depleted of ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB, E6310L) and 500 ng of the remaining RNA was purified, fragmented, and used for cDNA synthesis and library preparation. The Illumina sequencing library preparation was done using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, E7760L) according to manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM4292944