title: RNA-Seq of Acheta domesticus: 1 day nymph
design: Three biological replicates for each life stage were flash frozen in liquid N2 and were stored at -80oC.  Total RNA was extracted from all samples using Tri-reagent and Direct-zol kit (Zymo Research, Irvine, CA USA). Libraries were constructed, barcoded, and quantitated on NeoPrep (Illumina, San Diego, CA USA) using a NeoPrep library kit.  Barcoded libraries were pooled and sequenced on MiSeq (Illumina, 2 x 300 paired-end).
library_name: RNA-Seq of Acheta domesticus: 1 day nymph