title: GSM4087899: XX 2; Mus musculus; RNA-Seq
library_construction_protocol: Auxillary, brahcial, and inguinal draning lymph nodes were collected and processed into single cell suspensions. Cells were sorted for CD4+ T cells by magnetic negative selection using EasySep Mouse CD4+ T cell isolation kit (STEMCELL). The cells were cultured in the presence of anti-CD3/CD28 DynaBeads for 36 hours. RNA was isolated from CD4+ T cells using the Direct-zol™ RNA MiniPrep Plus kit (Zymo Research). Double-stranded cDNA and the sequencing library were made using the Kapa RNA Kit Full kit.
attributes.tag: GEO Accession
attributes.value: GSM4087899