title: GSM2175155: PMM [miRNA-seq]; Sus scrofa; miRNA-Seq
library_construction_protocol: Total RNA was extracted from adipose tissues using TRIzol (Invitrogen) and further purified with RNeasy columns (Qiagen) according to the manufacturer's protocol. About 45 μg of total RNA from the two tissues were used for miRNA transcriptome library preparation and sequencing. In briefly, only about 10–40 nt short RNAs were isolated from polyacrylamide gel electrophoresis (PAGE) was combined with adaptors (Illumina, San Diego, CA, USA) and converted to cDNA by RT-PCR. Then the cDNA was single-end sequencing in 36 bp on the Genome Analyzer GA-2 (Illumina) following the recommended protocol for small RNA sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2175155