title: RNA-Seq of homo sapiens: adult alveolar macrophages: Sample S47
design: "A single-cell suspension was prepared from a sample of whole lung tissue. Using flow cytometry, alveolar macrophages were identified using sequential gating as singlets/CD45+/live/CD15-/CD206++/CD169+/HLA-DR+/high autofluorescence. RNA was extracted and RNA-Seq libraries were prepared using a poly(A) enrichment (NEBNext Ultra RNA, New England BioLabs) and sequenced as single-end 75 base pair reads (Illumina NextSeq 500)."
library_name: Sample 47