title: GSM4514043: ATAC-seq_Fli1KO_rep1; Mus musculus; ATAC-seq
library_construction_protocol: Sorted cells (2.5 x 104) were washed  twice in cold PBS and resuspended in 50μl of cold lysis buffer (10nM Tris-HCl, pH 7.4, 10mM NaCl, 3mM  MgCl2, 0.1% Tween). Lysates were centrifuge (750xg, 10min, 4oC) and nuclei were resuspended in 50μl of  transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl];  (Illumina)) and incubated for 30min at 37oC. Transposed DNA fragments were purified using a Qiagen  Reaction MiniElute Kit, barcoded with NEXTERA dual indexes (Illumina) and amplified by PCR for 11  cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM4514043