title: GSM2935164: kdm5-WT 5; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was extracted from 3rd instar larval wing discs using Trizol and RNAeasy (Qiagen). Libraries were prepared using the TruSeq Stranded mRNA Library Preparation Kit in accordance with the manufacturer's instructions. Final libraries were evaluated using fluorescent-based assays including PicoGreen (Life Technologies) or Qubit Fluorometer (invitrogen) and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100), and were sequenced on an Illumina HiSeq2500 sequencer (v4 chemistry) using 2 x 50bp cycles.
attributes.tag: GEO Accession
attributes.value: GSM2935164