title: GSM2857475: S2 rep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from cell lines using a trizol and alcohol-based protocol. Coding and non-coding RNA was enriched by depleting ribosomal RNA (rRNA) using the Ribo-minus kit (ThermoFisher-order no. A15026). RNA quality and efficiency of rRNA depletion were ascertained using an Agilent 2100 Bioanalyzer with RNA 6000 Nano LabChip kit (Agilent Technologies-order no 5067-1511). cDNA libraries were generated using a NEBNext kit (New England Biolabs- order no. E6040s).
attributes.tag: GEO Accession
attributes.value: GSM2857475