title: GSM4512025: IIa_2; Homo sapiens; RNA-Seq
library_construction_protocol: The 9 patients of Uygur women were hospitalized in First Teaching Hospital of Xinjiang Medical University. Each sample was divided into two parts: one part was put into liquid nitrogen within 10 minutes after separation, and then stored at −80°C, and the other part was used for pathological diagnosis after hematoxylin-eosin (HE) staining. Total RNA was obtained from PBNCs using RNAprep Pure Blood Kit (Tiangen Biotech, Beijing, China) following the manufacturer's procedure. The RNA concentration and purity werechecked by OD A260/A280 (> 1.8) and A260/A230 (> 1.6), and the yield and quality were accessed using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), RIN number > 7.0. For each sample, 10μg of the total RNA was used for RNA-seq library preparation. Polyadenylated mRNAs were purified and concentrated with oligo (dT)-conjugated magnetic beads (Invitrogen, Carlsbad, CA, USA) before directional RNA-seq library preparation. The purified mRNAs were then iron fragmented at 95°C followed by end repair and 5' adaptor ligation. Then, reverse transcription was performed with RT primer harboring 3' adaptor sequence and randomized hexamer. The cDNAs were purified, amplified, and stored at -80°C until they were used for sequencing. For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions. Illumina NextSeq 500 system was used to collect data from 151-nt pair-end sequences (ABLife Inc., Wuhan, China).
attributes.tag: GEO Accession
attributes.value: GSM4512025