title: GSM4512070: RNA_NELFBKO_2; Mus musculus; RNA-Seq
library_construction_protocol: Nuclear run-on using permeabilized cells.  Biotin-11-NTPs were incorporated into nascent transcripts during run-on and total RNA was isolated using a column based total RNA isolation kit. PRO-seq (Precision nuclear run-on).  Biotinylated RNA from nuclear run-on was fragmented before enrichment on streptavidin magnetic beads. Illumina compatible ssRNA adapters were ligated sequentially to the RNA ends.
attributes.tag: GEO Accession
attributes.value: GSM4512070