title: RNA-seq of Solenopsis invicta: Whole body from a single polygyne worker
design: We extracted RNA and DNA from each sample using a dual DNA/RNA Tri Reagent based protocol. We applied the Krieger and Ross (2002) assay on the extracted DNA to identify only individuals with the SB/Sb genotype. Once RNA was extracted, cDNA libraries were prepared from total RNA using half volumes of the NEBNext Ultra II RNA Library Prep Kit for Illumina. Raw RNA and the libraries were quality checked on an Agilent Tapestation 2200; library insert size averaged 350 bp.
library_name: 104W