title: GSM3105089: S40_H3K9me3_ChIPSeq; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-Seq, 3 replicate pellet were prepared for each sample. Pellets consisted of at least 5 million cells, cross linked with 1% formaldehyde in phosphate buffered saline for 7 minutes before quenching with 125mM glycine for 5 minutes. Cells were pelleted before flash freezing and storing at -80C. Pellets were then shipped on dry ice to Zymo Research for ChIP preparation. For RNA-Seq, total RNA was extracted from three replicate cultures of each sample using the Machery Nagel Nucleospin RNA Plus kit according to the manufacturers recommendations. RNA samples were shipped on dry ice to Novogene Corporation for quality control analysis followed by rRNA depleted total RNA-Seq. Libraries for ChIP-Seq and RNA-Seq were prepared using the services provided by Zymo Research and Novogene Corporation respectively.
attributes.tag: GEO Accession
attributes.value: GSM3105089