title: GSM4199193: mut-16(pk710) Mutant Dissected Gonads mRNA 20C, Rep 3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: sample collection: Dissected gonads were collected in Trizol on ice over the course of 8-10 hours, then stored at -80 C. The Trizol containing the gonad tissue underwent 2 freeze/thaw cycles using liquid nitrogen prior to Trizol/chloroform extraction. RNA was depleted of rRNA using RiboZero and enriched for >200nt sizes with Zymo RNA clean and concentrator-5. Libraries were then prepared according to the NEBNext® Ultra™ II Directional RNA kit protocol. Size selection and library purification was done using AMPure XP beads.
attributes.tag: GEO Accession
attributes.value: GSM4199193