title: GSM4368628: 9hr_02_positive; Mus musculus; ATAC-seq
library_construction_protocol: Lungs were removed, single suspension cells were achieved by enzymatic digestion of tissue, and alveolar macrophage cells were harvested by FACS. Omni-ATAC was performed on 50,000 cells from two biological replicates per AM subpopulation using Tn5 transposase from the Nextera DNA Library Prep Kit (FC-121-1030). Following transposition, the Qiagen MinElute Kit (28204) was used to purify the DNA. Based on qPCR amplification curves, samples were amplified for four additional cycles after the five-cycle preamplification (9 total cycles). For size selection and clean-up of amplified library, three rounds of AMPure beads-enabled purification were performed. 1X AMPure beads were used first to remove primer dimers, followed by 0.5X to remove large fragments >1,000 kb, and finally 1.8X to further clean up.
attributes.tag: GEO Accession
attributes.value: GSM4368628