title: GSM3874615: S14 RPM; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was extracted from FFPE samples using the Qiagen miRNeasy kit (Qiagen, Germany). RNA was eluted in 30 µl nuclease-free water, quantified using the RiboGreen RNA assay, and 200 ng total RNA were used for library preparation. Ligation of 3' and 5' adapters, reverse transcription and PCR amplification (17 cycles) was performed using the NEBNext small RNA Kit. Libraries were pooled using equimolar amounts of miRNA peaks, followed by gel purification of 16-50 nucleotide (nt) fragments.