title: GSM2695612: IMS-M2 RNA-Seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using Qiagen AllPrep kit per manufacturer’s instructions. RNA sequencing libraries were prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (HMR) (KAPA Biosystems). Libraries were constructed following the manufactures protocol using 300ng of total RNA. RNA was fragmented to a size of 100-200 nucleotides and amplified for 11 cycles. Final libraries were quantified on a high sensitivity bioanalyzer chip and sequenced at 1.6 pM on a High Output Flowcell on the Illumina NextSeq 500.
attributes.tag: GEO Accession
attributes.value: GSM2695612