title: GSM4453382: 24 somite; Mus musculus; RNA-Seq
library_construction_protocol: A single embryo was dissected under a stereomicroscope, and a pair of optic areas was dissected manually using a fine needle (Austerlitz Insect Pins with a diameter of 0.1 mm). The dissected tissues were incubated with 0.05% Trypsin-EDTA for 3 min at 37°C. Then, 10% fetal calf serum/minimal essential medium was added to stop the proteinase reaction. The cells were dissociated by gentle pipetting and suspended in 0.04% bovine serum albumin/phosphate-buffered saline (PBS). The number of cells was counted using the Countess II FL automated cell counter (Invitrogen, Carlsbad, CA, USA). 2,000 cells/sample were then prepared as scRNA-seq library using Chromium Single Cell 3' Reagent Kits v3 (10x Genomics, Pleasanton, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM4453382