title: 16S rRNA pyrosequencing of microbial community
design: Variable regions 5 to 7 (V5 V7) of bacterial 16S rRNA gene were amplified with the specific primers 799F and 1193R. The PCR products of each sample were pooled in equimolar concentrations and purified using a Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated with TruSeq  DNA PCR-Free Sample Preparation Kit (Illumina, USA) following the manufacturer's instructions, and then sequenced on an Illumina HiSeq2500 platform.
library_name: MUP