title: metabarcoding of complex plant food product: tea: Sample plant_food_product_T2
design: "The two-step PCR method was used for library preparation, in a way similar to protocol in work of [Speranskaya et al., 2018], but with new optimized primers that selectively anneal to 18S and 5.8S sequences of plants, excluding fungi. Encyclo Plus PCR kit (Evrogen, Russia) was used for nrITS1 amplification. Illumina libraries were sequenced on the HiSeq2500 with Hiseq Rapid v. kit, 500 cycles (Illumina, USA) with 251 + 251 cycles setting."
library_name: T2-2_Enc