title: GSM3768234: Lgr5+ ISC Input; Mus musculus; ChIP-Seq
library_construction_protocol: Proximal 1/3 of small intestine were resected, and crypts were isolated by villi scraping and 5mM EDTA incubation. Cells were dissociated with 4x TrpLE in DMEM for 45 minutes, and Lgr5-EGFPhi cells were sorted by flow cytometry. For ChIP-seq, whole cell extracts from sorted Lgr5-EGFPhi cells fixed with 1% formaldehyde were sonicated and incubated overnight with H3K27ac antibody (#39133, Active Motif) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries.
attributes.tag: GEO Accession
attributes.value: GSM3768234