title: Illumina HiSeq 2000 sequencing; RNAseq of Human Umbilical Vein Endothelial Cells treated with Insulin in the presence or absence of TGF-beta kinase inhibitor SB431542 for 90 minute.
design: RNAseq of Human Umbilical Vein Endothelial Cells treated with Insulin in the presence or absence of TGF-beta kinase inhibitor SB431542 for 90 minute.
library_name: Insulin and inhibitor SB431542 sample #3_s
library_construction_protocol: HUVECs were bought from GIBCO company HUVECs were grown in Medium 200 (ThermoFisher Catalog # M200500), 10 % Fetal Bovine Serum from HyClone company, and addition of LSGS (ThermoFisher Catalog # S-003-10) HUVECs were starved 6 hours to overnight before start of the experiment.Cell were pretreated with 5 micromolar SB431542 for 40 minutes before addition of 100 nanomolar insulin for 90 minutes. RNA was extracted using RNeasy Kit Plus Encore Complete RNA-seq DR Multiplex (Nugen Catalog # 0333-32)
attributes.tag: Experimental Factor: stimulus
attributes.value: insulin, 100 nanomolar
attributes.tag: Experimental Factor: compound
attributes.value: SB431542
attributes.tag: Experimental Factor: dose
attributes.value: 5
attributes.tag: Experimental Factor: time
attributes.value: 90