title: RNA-Seq of Pristimantis toftae: adult male liver
design: Total mRNA was extracted from the samples using the QIAGEN miRNAeasy kit. Quantity and quality of the extracted total mRNA was assessed with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific) and Experion Labchip electrophoresis (BIORAD). Illumina Tru-Seq libraries for polyA-tail mRNA were prepared following manufacturers guidelines. Raw reads were processed for quality, read size and read number using FastQC v0.11.2 (Babraham Bioinformatics) and Trimmomatic (Bolger et al. 2014) including adapter trimming.
library_name: H10