title: GSM3399574: SSB_Low-RNAse-LMW-rep2; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were UV crosslinked and lysates were treated with low RNAse or no RNAse before IP by anti-SSB antibody HITS-CLIP for endogenous SSB was done as described by (Licatalosi et al., 2008) with the modifications previously used for YBX1 small RNA CLIP (Goodarzi et al., 2015). MDA-MB-231 cells were UV-crosslinked at 400 mJ/cm2 before cell lysis. Samples with and without RNase treatment were immunoprecipitated with an anti-SSB antibody (MBL, RN074PW) for protein-RNA complexes. Polyphosphatase (Epicentre) was incubated with smRNA samples before ligation and PCR amplification with primers described by (Goodarzi et al., 2015).
attributes.tag: GEO Accession
attributes.value: GSM3399574