title: WGS of Arabidopsis thaliana: pooled DNA from F2 cross
design: From each individual the apex of the main stem was collected onto dry ice shortly after bolting and stored at -80C. Apices from the bulk were pooled and ground together in liquid nitrogen. DNA was extracted using a DNeasy Plant Maxi kit (QIAGEN). Genomic DNA library preparation was carried out using TruSeq DNA PCR-Free LT Library Prep kit (Illumina 20015962). The libraries were sequenced on a NextSeq500 machine using the NextSeq 500/550 High Output Kit v2.
library_name: M182xCol_bulk_L2