title: GSM3033402: 6h_STM-D23580_D1_277151; Homo sapiens; RNA-Seq
library_construction_protocol: 5'000 live cells were sorted in eppendorf containig 10uL of PBS. RNA was extracted with Qiagen RNeasy Micro kit and 1 uL of RNA was added to 4uL of lysis buffer (0.4% Triton, 2U/uL RNase inhibitor (Clonotech, Takara), 7.5uM oligo dT (Biomers) and 10mM dNTP [supplemented with ERCC spike-in 1:10,000,000] (Invitrogen)) and processed as for single cells. Nextera XT DNA sample preparation kit with the dual index strategy
attributes.tag: GEO Accession
attributes.value: GSM3033402