title: skin tissue of A. davidianus sequencing
design: After RNA quality control, small RNAs of 16-30 nt in length were isolated from total RNA by size fractionation with 15% TBE urea polyacrylamide gel and then adaptors were ligated to the 5′ and 3′ terminals of these small RNAs. Afterwards, reverse transcription followed by RT-PCR was used to create cDNA constructs, which were then purified on 6% Novex TBE PAGE gel and sequenced at the Beijing Biomarker Technologies Co., Ltd. using Illumina HiSeq 2500 platform. Sequences were extracted from image files using the Illumina pipeline.
library_name: College of Animal Science and Technology, Henan University of Science and Technology