title: GSM1977557: GSCLC_1_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from GSCs and GSCLCs by using an RNeasy Micro Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. Synthesis and amplification of cDNAs using 1 ng of purified total RNA, and construction of cDNA libraries for RNA sequencing (ABI SOLiD 5500XL system; Life Technologies), were performed as described in Nakamura et al., 2015. cDNA synthesis and amplification from isolated RNAs/single cells were performed essentially as reported in Kurimoto, K.et al., Nucleic Acids Research, 34(5), e42. Library constructions for SOLiD System were performed as reported in Nakamura et al., Nucleic Acids Res. (2015).
attributes.tag: GEO Accession
attributes.value: GSM1977557