title: GSM1977857: Dsim_YVF_repC; Drosophila simulans; RNA-Seq
library_construction_protocol: Female LIII larvae were dissected and eye-antennal imaginal discs were stored in RNALater at 120 h after egg laying. 40-50 discs were dissected per sample. Total RNA was isolated using the Trizol  and the samples were DNase I treated in order to remove DNA contamination. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit starting from 500 ng of total RNA. Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™dsDNA System . The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced by using cBot and HiSeq 2000 (Illumina):single-end reads were generated for D. mauritiana (replicates A, B and C) and for D. melanogaster samples (1x50 bp) and paired-end reads were generated for D. mauritiana (replicates D, E and F) and for D. simulans samples (2x100 bp).
attributes.tag: GEO Accession
attributes.value: GSM1977857