title: WES of Homo sapiens: blood: Sample XH2355-U
design: Whole exome sequencing was carried out on genomic DNA of 1024 cases and 1004 controls at the Human Genome Sequencing Center (HGSC) at Baylor College of Medicine in 4 waves. Illumina paired-end pre-capture libraries were constructed using 0.5ug of DNA according to the manufacturer's protocol (Illumina Multiplexing_SamplePrep_Guide_1005361_D) with modifications as described in the HGSC protocol (https://www.hgsc.bcm.edu/content/protocols-sequencing-library-construction). For each capture hybridization, six pre-capture libraries were pooled and then hybridized in solution to the HGSC VCRome 2.1 design (32) (42Mb, NimbleGen) essentially according to the manufacturer's protocol NimbleGen SeqCap EZ Exome Library SR User's Guide (Version 2.2). Sequencing was performed using the Illumina HiSeq 2000 platform in paired-end mode, with sequencing-by-synthesis reactions extended for 101 cycles and an additional 7 cycles for the index read. Primary sequence data analysis was performed using the HGSC Mercury analysis pipeline (https://www.hgsc.bcm.edu/software/mercury). The whole exome sequence data was mapped to GRCh37/hg19 human reference genome with BWA-aln v.0.6.235. Aligned reads were further processed by PICARD v1.93 (broadinstitute.github.io/picard) and GATK v2.5-2 pipeline (34).
library_name: Homo-Sapiens-UW-XH2355-U
attributes.tag: alignment_software
attributes.value: BWA-aln v.0.6.235