title: bTEFAP of hot water spring: bacterial and archaeal diversity
design: The 16S rRNA gene V4 variable region PCR primers with barcode on the forward primer were used in a 28 cycle PCR After amplification Multiple samples are pooled together in equal proportions based on their molecular weight and DNA concentrations. Pooled samples are purified and final PCR product is used to prepare illumina DNA library.
library_name: MBSS2_HWS_AMP