title: Illumina HiSeq 2500 sequencing; MAFB ChIPseq in Human EndoCBH2 cells
design: MAFB ChIPseq in Human EndoCBH2 cells
library_name: IgG control sample_s
library_construction_protocol: Chromatin was prepared from EndoC-βH2 cells by cross-linking cells for 10 minutes with 1% formaldehyde. EndoC-βH2 cells were grown in DMEM containing 5.6 mM glucose, 2% BSA, 50 μM 2-mercaptoethanol, 10 mM nicotinamide, 5.5 μg/mL transferrin, 6.7 ng/mL selenite, 100 units/mL penicillin, and 100 units/mL streptomycin (Scharfmann et al., 2014). Cells were collected and lysed in SDS buffer (Millipore Chromatin Immunoprecipitation Assay Kit. Cat#17-295), and chromatin was sonicated using a Diagenode Bioruptor. Standard DNA sequencing library for sequencing on Illumina sequencing platforms.
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: IgG