title: GSM3874412: PHH_D1_HCV6h; Homo sapiens; RNA-Seq
library_construction_protocol: PHH were lysed in 1 ml Trizol reagent (Invitrogen) and lysates stored at -80°C until processing. We extracted the total RNA and DNA according to the manufacturer's instructions and added 10-20 µg glycogen per sample to facilitate RNA precipitation. Total RNA of other cells than PHH was extracted from living cells scrapped in RA1 Lysis buffer 1% beta Mercaptoethanol and isolated using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer's instructions. RNA sequencing library generated with ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina)
attributes.tag: GEO Accession
attributes.value: GSM3874412