title: PacBio DNA-seq of Agrocybe pediades:  mycelium
design: The PacBio libraries were produced using the SMRTbell Template Prep Kit 1.0 according to manufacturer's instructions. 10 g of genomic DNA per library were sheared into 20 kb fragments using the Megaruptor system (Diagenode), followed by an exo VII treatment, DNA damage repair and end-repair before ligation of hair-pin adaptors to generate a SMRTbell library for circular consensus sequencing. The library was then subjected to exo treatment and PB AMPure bead wash procedures and was size selected with the BluePippin system with a cut-off value of 9000 bp.
library_name: AGP102.39_GD7