title: GSM3400502: Small RNAs from ovaries of CCR4/+; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: small RNAs: Total RNA was isolated by Trizol (Invitrogene) and size-fractioned by PAGE (19-29 nt); chromatin bound RNA: Nuclear RNA was extractred as descirbed in (Chen et al 2016, PMID: 27292797). small RNAs: Libraries of small RNAs 19-29 nt were constructed as previously described in (Muerdter et al. 2012); chromatin bound RNAs: cDNA was synthesized using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen). Reaction of tagmentation was performed in according to previously published protocol (Picelli et al. 2014) using in-house produced Tn5 charged with oligonucleotides compatible with the Illumina system. Reaction products were cleaned using Clean Mini (Evrogen), PCR amplified and sequenced on the Illumina HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM3400502