title: GSM2357672: Tolerized KB5 (DST+antiCD154), rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was recovered from the purified cells. Extraction of total RNA from cultured mouse embryonic fibroblasts was performed using the RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. DNA was eliminated by on-column RNase-free DNase treatment. RNA quality was verified (RNA integrity number >8.5) using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Messenger RNA was isolated, converted to cDNA, and amplified using the mRNA Purification Kit (Illumina, San Diego, CA) according to the manufacturer's instructions. An Illumina library was built and used to generate clusters on the Illumina flow cell according to the Illumina protocol and sequenced using a HiSeq 2000 (Illumina). Three independent libraries were examined for each condition.
attributes.tag: GEO Accession
attributes.value: GSM2357672