title: GSM4200358: HF_A; Capra hircus; RNA-Seq
library_construction_protocol: For each group skin tissues were obtained from at least 3 independent female, prior to digestion. To prepare dorsal skin single cell suspension for single-cell RNA sequencing, the back skin tissues were isolated via microdissection and 0.25 % trypsin/EDTA solution was used to digest dorsal skin tissues at 37 °C for 30 min，mechanically dissociate the tissue once every 10 min. 1 mg/ml collagenase IV (Sigma, St Louis, MO, USA) was used to digest the tissue which digested by trypsin at 37 °C for 30 min. the skin tissues were mechanically dissociated into single cell suspension by pipetting, the cell suspensions were then filtered through a 30 μm nylon cell strainer, to remove villus debris(BD Falcon, BD Biosciences, San Jose, CA, USA) prior to single cell library construction. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237) following the manufacture's introduction.
attributes.tag: GEO Accession
attributes.value: GSM4200358