title: Fibrosis
design: Adult rats and experimental procedures were carried out in compliance with the Guide for the Care and Use of Laboratory Animals of the Chinese Academy of Sciences and approved by the Medicine Animal Care Committee of Xiangya Medical College, Central South University (Changsha,Hunan PR,China). Eight-week-old Sprague–Dawley male rats (120 g～150 g; from Department of Laboratory Animal Science, Xiangya Medical College, Central South University, Changsha, Hunan PR, China) were used in this study. Six male rats were divided into two groups (normal controland ﬁbrosis model; n = 3 in each group). The fibrosis model group was injected intraperitoneally at a dose of 0.5 ml porcine serum twice a week for 12 weeks[23, 28]. The normal control group received only saline(NS) in the same way. At the end of twelfth weeks,  all rats were sacrificed for other experiments. Liver tissues were frozen in refrigerator after surgical removal and stored at -80 ℃ for further studies.