title: NextSeq 500 paired end sequencing; Whole-exome sequencing (WES) of single glands from opposite side of two colorectal tumors and WES of colorectal cancer xenografts derived from single cell culture to study tumor growth dynamics
design: Whole-exome sequencing (WES) of single glands from opposite side of two colorectal tumors and WES of colorectal cancer xenografts derived from single cell culture to study tumor growth dynamics
library_name: OA2_p
library_construction_protocol: This study employed deidentified excess tissue specimens collected in the course of routine clinical care and was approved by the local institutional review board (IRB). Individual tumor glands composed of <10,000 adjacent cells were isolated from fresh colectomy specimens following EDTA treatment. DNA was isolated from individual glands by incubation in 15 µl of Tris-EDTA solution with Proteinase K solution (4 h at 56 °C), and reactions were boiled for 5 min. illumina Nextera Rapid Capture Exome Kit
attributes.tag: Experimental Factor: not applicable
attributes.value: cell line
attributes.tag: Experimental Factor: not applicable
attributes.value: growth condition
attributes.tag: Experimental Factor: none
attributes.value: xenograft
attributes.tag: Experimental Factor: tumor gland
attributes.value: organism part
attributes.tag: Experimental Factor: not applicable
attributes.value: sampling time point