title: 16Samplicon:groundwater:enrichment_culture
design: DNA was extracted with the FastDNA Spin Kit for Soil (MP Biomedicals). Two rounds of PCR were applied for DNA amplification. The first PCR was performed using the KAPA HiFi ReadyMix PCR Kit (KAPABioSystems, Cape Town, South Africa) and universal primer-tailed 16S primers (27F27F /5AmMC6/ gcagtcgaacatgtagctgactcaggtcac AGRGTTYGATYMTGGCTCAG, 14292R /5AmMC6/ tggatcacttgtgcaagcatcacatcgtag RGYTACCTTGTTACGACTT) (Eurofins Genomics, Ebersberg, Germany), followed by purification with the QIAquick PCR purification kit (QIAGEN, Hilden, Germany). The second PCR was performed using the KAPA HiFi ReadyMix PCR Kit and PacBio Barcoded Universal F/R Primers Plate  96 (Pacific Biosciences, California, USA), followed by AMPure PB bead kit (PacBio biosciences, California, USA) purification. The SMRTbell library preparation and further purification were achieved by SMRTbell Template Prep Kit (PacBio biosciences, California, USA).
library_name: 1N1