title: GSM4509056: gRNA library1 HeLa in vitro D30 replicate2; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA of screening cell samples were extracted with DNA EXTRACTION Kit (Tiangen)according to the manufacturer's protocol. For gRNA library analysis, genomic DNAs from HT29, 293FT and HeLa cells for screens were extracted. gRNA library was enriched by PCR amplification. Deep sequencing of amplified PCR products containing gRNA sequences was conducted by Illumina NextSeq X ten at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China.
attributes.tag: GEO Accession
attributes.value: GSM4509056