title: GSM4143760: D10 rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from two independent experiments was prepared using Trizol reagent. 1 ug of total RNA was used for construction of sequencing libraries. PolyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments using Illumina's TruSeq Stranded Library Prep Kit. Adapter-ligated library was completed by PCR with Illumina PE.
attributes.tag: GEO Accession
attributes.value: GSM4143760