title: GSM4294225: KO2-Lesion1; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were harvested, embedded in OCT and cut into frozen sections on a Leica CM3050 S Research Cryostat equipped with Cryojane. GFP and RFP+ Lung lesions were extracted by laser capture microdissection on a Leica LMD7000 instrument instrument. RNA was purified by RNeasy micro kit (Qiagen). 2ng of total RNA per sample was used to prepare the sequencing libraries. Libraries were then prepared using QIAseq FX single cell RNA library kit (Qiagen) following standard manufacturer's protocol. The cDNA library was inspected using using Agilent Bioanalyzer High Sensitivity DNA chips and quantitated by PicoGreen dsDNA Assay kit (ThermoFisher).
attributes.tag: GEO Accession
attributes.value: GSM4294225