title: Illumina HiSeq 2000 paired end sequencing; Abiotic stress-response reprogramming events underlie successful initiation of apomixis
design: Abiotic stress-response reprogramming events underlie successful initiation of apomixis
library_name: Sex-1_p
library_construction_protocol: 17-18 days old seedlings from Sex-1 and Apo-1 accessions were used. Seedlings were snap-frozen and kept at -80ºC until RNA extraction Plants were grown in a dedicated plant growth chamber with 16-hours photo period/8-hours darkness cycle, 60-65% humidity and 22ºC. Leaves from all the plants were ploidy genotyped either in a Partec flow cytometer (Sysmex, Germany) or in a Guava® easyCyte flow cytometer (Millipore, USA).  RNA Extraction was performed using QIAGEN RNeasy Mini Kit and DNase–treated according to manufacturer’s instructions (Qiagen, USA). The RNA quality was further validated in a Bioanalyzer (Life Technologies, USA). library preparation was performed as per the routine pipeline established at the facility of Fasteris
attributes.tag: Experimental Factor: apomixis reproduction
attributes.value: phenotype