title: GSM4368674: NIR_Gli1_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP done and DNA extracted using SimpleChIP Plus kit (Cell Signaling Technologies, 9005S) Done by external vendor (GENEWIZ). Library was constructed with NEBNext Ultra DNA Library Prep with Beads Size Selection following manufacturer's recommendations. Briefly, the ChIP DNA was end repaired and adapters were ligated after adenylation of the 3'ends. Adapter-ligated DNA was size selected, followed by clean up, and limited cycle PCR enrichment. The ChIP library was validated using Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as real time PCR (Applied Biosystems, Carlsbad, CA, USA). ChIP-Seq done by external vendor (GENEWIZ). The sequencing libraries were multiplexed and clustered on one lane of a flowcell. After clustering, the flowcell was loaded on the Illumina HiSeq instrument according to manufacturer's instructions (Illumina, San Diego, CA, USA).  Sequencing was performed using a 2x150 Paired End (PE) configuration.  Image analysis and base calling were conducted by the HiSeq Control Software (HCS). Raw sequence data (.bcl files) generated from Illumina HiSeq was converted into fastq files and de-multiplexed using Illumina's bcl2fastq 2.17 software. One mis-match was allowed for index sequence identification.
attributes.tag: GEO Accession
attributes.value: GSM4368674