title: RAD-Seq of Malus domestica: young leaves
design: Genomic DNA was extracted from young leaves. It was then digested using the ApeKI restriction enzyme. ApeKI-specific adapters tagged with different barcodes were ligated to the restriction fragments. The resulting products were amplified and checked for quality. Samples were pooled and run in a single lane on an Illumina HiSeq instrument. The resulting sequences were finally assigned based on the correspondence between the barcodes and the samples. Keygene N.V. owns patents and patent applications protecting its Sequence Based Genotyping technologies.
library_name: H3T5WBBXX_5_A3-30-10-41