title: GSM1155407: small RNA-seq of rdr2 x rdr2 seeds; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted using Plant RNA Reagent (Invitrogen, http://www.invitrogen.com/). Small RNA libraries were prepared as previously described.  In brief, an aliquot of 10 µg of total RNA was resolved in a 15% urea-polyacrylamide gel, and the fraction of 18-30-nt small RNAs was recovered.  For nrpd1a and rdr2 mutants and their crosses, whole seeds dissected from ~20 siliques were used for each library. For manually dissected seed, the embryo, endosperm and seed coat dissected from ~50 siliques were used for each library.  Purified small RNAs were ligated to 5' and 3' RNA oligo adapters and reverse transcribed to produce first-strand cDNAs.
attributes.tag: GEO Accession
attributes.value: GSM1155407