title: Transcriptome Profiling of Phaseolus vulgaris after rhizobia and AMF inoculation
design: High quality total-RNA was isolated from the frozen tissues of three biological replicates using ZR Plant RNA MiniPrep following the manufacturer's instructions (Zymo Research, USA). Contamination of genomic DNA was eliminated by incubating the samples with RNAase-free DNAase (1 U um -1) at 37 C for 15 min. The RNA Integrity Number (RIN) (Kashofer et al. 2013) and concentration of RNA samples, were determined using an Agilent 2100 Bioanalyzer platform (Agilent Technologies, Santa Clara, CA, USA), with a Nano 6000 Kit. Only samples with RIN values of 8.0 or above were used for RNA-seq analysis. cDNA libraries for each sample were generated separately using the TruSeq RNA-Seq Sample Prep kit (Illumina Inc., San Diego, CA, USA) according to the manufacturers protocol. Libraries were sequenced with the Illumina HiSeq2000 (Illumina Inc.) as paired-end reads.
library_name: PvRbohB-RNAi_Myc_2