title: GSM1155654: cuff mutant rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Small RNA libraries from the cn bw control line, and from cuffwm25 homozygous ovaries were produced with the Small RNA Library Prep Kit (Illumina). Briefly, 20 μg total RNA extracted from the different genotypes was separated on a 15% denaturing polyacrylamide/urea gel electrophoresis (Invitrogen) for 1 h at 200 V. A gel slice corresponding to 18–30-nt-long RNAs was isolated and small RNAs were eluted and processed with the Small RNA Library Prep Kit as per the manufacturer's instructions. Small RNA libraries were subsequently sequenced on the Solexa Genome Analyzer II platform.
attributes.tag: GEO Accession
attributes.value: GSM1155654