title: RNA-Seq of R. tropici CIAT 899: salt rp2
design: Second replicate of the whole transcriptome of Rhizobium tropici CIAT 899 grown under salt condition. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and also under salt stress conditions. RNA-seq experiments were carried out to identy genes that are differentially expressed in the presence of both inducing molecules. Sequentiation of these RNAs were conducted by Sistemas Genómicos with platform Illumina using the Illumina HiSeq 2000 sequencing instrument. Ribosomal-depleted samples were used to generate whole transcriptome libraries following the manufacturer's recommendation for sequencing on this NGS platform. The initial whole transcriptome paired-end reads obtained from sequencing were mapped against the latest version of the R. tropici CIAT 899 genome using the the Life Technologies mapping algorithm version 1.3. Low-quality reads were eliminated using Picard Tools software version 1.83, remaining only the high quality reads.
library_name: 04331699