title: Raw reads of Phaseolus Vulgaris breeding line MIB 778
design: Total genomic DNA was extracted from 1 g of leaf tissue of 30 day old plants using liquid nitrogen and the Urea buffer based DNA extraction midi prep protocol (Chen et al., 1992). DNA was quantified using NanoDrop 2000 (Thermo Fisher scientific, Waltham, USA) and the DNA quality was evaluated by electrophoresis and visualization on a 1% agarose gel, including digestions with EcoRI, PstI and RsaI enzymes. Sequencing libraries were prepared using the TruSeq DNA PCRFree Library preparation kit (Illumina, San Diego, USA) and sequencing was performed at the HudsonAlpha Institute for Biotechnology (http://hudsonalpha.org/)
library_name: WGS Bean MIB 778