title: Illumina MiSeq sequencing; qiita_ptid_2229:10778.S1182
design: Thirty-seven children were recruited from the Feeding Queensland Babies Study. At age 2 to 3 years faecal microbiota composition was examined by 16S rRNA sequencing while body composition was estimated using deuterium oxide dilution technique.
library_name: 10778.S1182
library_construction_protocol: DNA extracted using an initial bead beating step followed by extraction using the Maxwell 16 Research Instrument. Preparation of the 16S library was performed using the workflow outlined by Illumina.
attributes.tag: center_name
attributes.value: Children's Nutrition Research Centre, University of Queensland
attributes.tag: center_project_name
attributes.value: GMA
attributes.tag: experiment_title
attributes.value: Faecal microbiota is associated with fat free mass in 2 to 3 year old well-nourished Australian boys.
attributes.tag: linker
attributes.value: N/A
attributes.tag: pcr_primers
attributes.value: AAACTYAAAKGAATTGRCGG
attributes.tag: primer
attributes.value: N/A
attributes.tag: run_center
attributes.value: Australian Centre for Ecogenomics
attributes.tag: run_date
attributes.value: 10/21/2032
attributes.tag: run_prefix
attributes.value: J72_S125_L001_R1_001
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V6-8