title: RNA-Seq of R. pulchrum blooming flowers
design: The  Rhododendron pulchrum Sweet were grown in the Dabie mountain in Hubei Province. Total RNA was extracted from pepper after inoculation with different treatment tissues (buds,initial flowers,blooming flowers,withering flowers) and analyzed using RNA-seq for identifing different expressed genes among them. Total RNA were extracted from the tissues using an RNeasy Plant Mini Kit (QIAGEN) according to the manufacturers protocol. Messenger  RNAs  from the total RNA were isolated using Oligo (dT) and were randomly cleaved into short  fragments. Then the first strand cDNAs were synthesized with random hexamer primers and followed by second strand cDNAs synthesis. The cDNAs were purified and ligated to adaptors for paired-end sequencing. The quality and quantity of the libraries were detected using an Agilent 2100 Bioanalyzer and ABI real time RT-PCR. The qualified cDNA libraries were carried out by an Illumina HiSeq 2500 platform.
library_name: Blooming flowers_R. pulchrum