title: Illumina HiSeq 1500 paired end sequencing; Inter and intra-lineage genetic diversity of Zika virus differs for American isolates
design: Inter and intra-lineage genetic diversity of Zika virus differs for American isolates
library_name: Sample 13_p
library_construction_protocol: Stock viruses were obtained from the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) at the University of Texas Medical Branch and passaged once in monkey kidney Vero cells in minimum essential media MEM supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids, 100 U/ml penicillin – 100 μg/ml streptomycin, and 2% fetal bovine serum for amplification.   Viral RNA from two separate virus stocks per strain was harvested using the Viral RNA isolation kit (Qiagen) cDNA libraries were constructed using random hexamers with TruSeq RNA v2 (Illumina)
attributes.tag: Experimental Factor: strain
attributes.value: R 103451