title: Illumina HiSeq 2500 paired end sequencing; RNA-sequencing of human embryonic and fetal urinary bladder and genital tissues
design: RNA-sequencing of human embryonic and fetal urinary bladder and genital tissues
library_name: Sample 16_p
library_construction_protocol: Embryonic and fetal bladders and genital tissue were obtained by surgeons from terminated pregnancies after informed consent and with ethics approval. Samples were taken from weeks 7 (n=2), 7,5 (n=1), 8 (n=3), 9 (n=4) for the bladder tissues and from weeks 7 (n=3), 8 (n=3), 9 (n=3) as well as 10 (n=4) for the genital tissues, respectively.   Fresh tissues were dissected and immediately submerged in 5 volumes of RNAlater Stabilization Solution (Cat No. AM7020, Thermoisher Scientific, Waltham, MA), incubated at 4°C overnight and then transferred to -20°C. RNA was extracted by using TissueLyser LT (Cat No. 85600, QIAGEN, Hilden, Germany) and RNeasy Fibrous Tissue Mini Kit (cat. no. 74704, QIAGEN, Hilden, Germany). The RNA was measured both quantitatively and qualitatively using NanoDrop (Thermofisher Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent, Santa Clara, CA) instruments.   Paired-end libraries of 2x126 bp with poly-A selection according to Illumina TruSeq Stranded mRNA protocol.
attributes.tag: Experimental Factor: age
attributes.value: 8
attributes.tag: Experimental Factor: organism part
attributes.value: reproductive organ