title: GSM4453336: Group2_RRBS_4; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Tissues [sural nerve (flash frozen on dry ice, and DNA/RNA was harvested using Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). DNA and RNA quantity was assessed using a Qubit fluorometer, and the quality was evaluated using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Illumina TruSeq mRNA Sample Prep v2 kit (Catalog #s RS‐122‐2001, RS‐122‐2002; Illumina, San Diego, CA, USA) was used with 1 ug of total RNA for the construction of sequencing libraries. Briefly, DNA was digested with the MspI restriction enzyme and purified using phenol:chloroform extraction and ethanol precipitation, blunt-end digested, phosphorylated, and ligated into an adapter duplex with methylated cytosines. The ligated fragments were cleaned and size selected by an agarose gel. Bisulfite conversion was performed for selected fragments followed by PCR amplification and cleanup using AMPure XP beads. The Qubit assay and Agilent's High Sensitivity D1000 assay were used to quantify the libraries.
attributes.tag: GEO Accession
attributes.value: GSM4453336