title: GSM2936555: 14876; Mus musculus; RNA-Seq
library_construction_protocol: Mature α-cells from 3-month-old Glucagon-Venus mice, α-cells 30 days after DT from Glucagon-Venus; RIP-DTR mice, mature β-cells from 3-month-old Insulin-mCherry mice,α-cells ectopically expressing PDX1 from 3-month-old Glucagon-rtTA; TetO-Cre; R26YFP;CAG-Pdx1, α-cells expressing PDX1 30 days after DT from Glucagon-rtTA; TetO-Cre; R26YFP; CAGPdx1;RIP-DTR mice. Mice were DT-injected at 2-months of age. RNA from cells isolated by FACS was assessed for quality by Agilent bioanalyzer prior to library generation. RNA prepared using the Qiagen RNeasy Micro Kit. cDNA was prepared using the Qiagen QuantiTect Reverse Transcription Kit starting from 1μg of RNA
attributes.tag: GEO Accession
attributes.value: GSM2936555