title: GSM1977475: Dox_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated with the miRNAeasy Mini Kit (Qiagen) using on-column DNase digestion from 106 AIRE-Tet cells. Two independent biological replicates from each treatment were prepared from 500 ng of RNA with RIN >9. Sequencing libraries were generated with the TruSeq Stranded Total RNA LT (w/ Ribo Zero Gold) Sample Prep Kit (Illumina) according to the manufacturer's instructions with the exception that 12 PCR cycles were used for the DNA enrichment step. Paired-end sequencing (2 x 100 bp) was performed with Illumina HiSeq 2000, which generated 80-100 x 10^6 sequenced fragments per sample.
attributes.tag: GEO Accession
attributes.value: GSM1977475