title: RNAseq of Rm1021: nutrient rich media
design: rRNA depletion was performed with 1 g of total RNA and the Meta-Bacteria Ribo-Zero rRNA Removal kit (Mandel Scientific, Guelph, Ontario, Canada) at the Genome Quebec Innovation Center of McGill University. Final RNA was isolated with the RiboMinus Concentration Module and eluted with 17 l of Fragment, Prime, Finish buffer (Life Technologies, Inc.). The eluted RNA was then fragmented and prepared for cDNA synthesis. The TruSeq Stranded mRNA Sample Prep kit was used for cDNA synthesis, and then the quality of the library and fragment size were assessed with a LabChip, a LightCycler 480 II, and an Infinite M200 Fluorimeter (Roche, Mississauga, Ontario, Canada, and Tecan, Mannedorf, Switzerland).
library_name: Rm1021_LBMC