title: GSM4017067: wildtype 8h ATRi [WT_8H_S3]; Homo sapiens; RNA-Seq
library_construction_protocol: Wild type and MLL4 Q4092X MSCs  were extracted collected by centrifuging cell cultures, either untreated or at 8 and 24 hours after ATR inhibition. Cellular pellets were resuspended with TRIzol (Thermo Fisher cat. #15596026Ambion #15596018), and total RNA was extracted according to the manufacturer's instructions. Contaminating genomic DNA was removed by DNase (Qiagen cat. #79254) digestion. RNA quality and concentration were assed with the 2100 Bioanalyzer (Agilent cat. #G2939BA) and the Qubit fluorometer (Thermo Fisher cat. # Q33226), respectively. 3'-RNA-seq libraries were prepared by using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen cat. #015.24) and starting from 500 ng of total RNA. Libraries were sequenced as single reads of 50 bp with the Illumina HiSeq2500.
attributes.tag: GEO Accession
attributes.value: GSM4017067