title: 16S rRNA gene  sequencing of homo sapiens: cystic fibrosis lung sputum: BONCAT Labeled
design: gDNA derived from  sputum and sorted samples was submitted to the University of Minnesota  Genomics Center (UMGC) for 16S rRNA gene library preparation using a two-step  PCR protocol described previously (doi: 10.1038/nbt.3601). The V4 region was  amplified and sequenced on an Illumina MiSeq using TruSeq (v.3) 2x300  paired-end technology. FACS sheath  fluid and DNA extraction reagent control samples were also submitted for  sequencing. These control samples did not pass quality control steps due to  DNA content below detection thresholds, but were incorporated into downstream  analyses.
library_name: TW059_IN