title: GSM3445682: intron-DARP-EGFP line SUPT4H1 siRNA replicate 6; Homo sapiens; RNA-Seq
library_construction_protocol: 72 hours post transfection, total RNA was extracted with the RNEasy (Qiagen) kit from 1,000,000 cells per sample.  Either ERCC mix1 or mix2 external standards (Invitrogen) were diluted 1:100 in water and 2 ul was added to each lysate. Sequencing libraries were generated using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen).   200 ng RNA was used for library preparation for each sample.
attributes.tag: GEO Accession
attributes.value: GSM3445682