title: GSM4087788: S353_2_WT_3; Mus musculus; RNA-Seq
library_construction_protocol: bulk RNA extracted from the whole tissue: RNA was extracted from hippocampal lysates using PicoPure RNA Isolation Kit (Thermo Fisher Scientific) according to the manufacturer's protocol. for WT and MAVS-KO ICV-Veh and ICV-STZ mice: Total RNA library preparation and sequencing (500ng 2x125bp). RNA sequencing libraries were prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (kapabiosystems) in accordance with the manufacturer's instructions. Briefly, 500ng of total RNA was used for ribosomal depletion and fragmentation. Depleted RNA underwent first and second strand cDNA synthesis. cDNA was then adenylated, ligated to Illumina sequencing adapters, and amplified by PCR (using 9 cycles). Final libraries were evaluated using fluorescent-based assays including PicoGreen (Life Technologies) or Qubit Fluorometer (invitrogen) and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100), and were sequenced on an Illumina HiSeq2500 sequencer (v2 chemistry for Rapid Run) using 2 x 125bp cycles. muMT and WT mice: Library preparation and RNAseq for WT and muMT mice were carried out as described in the Illumina TruSeq Stranded mRNA Sample Preparation Guide, the Illumina NextSeq 500 System Guide (Illumina, Inc., San Diego, CA, USA), and the KAPA Library Quantification Kit – Illumina/ABI Prism User Guide (Kapa Biosystems, Inc., Woburn, MA, USA).
attributes.tag: GEO Accession
attributes.value: GSM4087788