title: GSM1977057: Shh_rep2; Mus musculus; RNA-Seq
library_construction_protocol: For purification of Nfatc1 expressing cells in hair pegs, embryonic back skin was removed and the whole skin was cut into 6-9 small pieces and placed, dermis side down, in a 0.25% trypsin solution (Gibco) at 37°C for 16 min. Single-cell suspensions were obtained by triturating the skin gently. Cells were then filtered with strainers (70um followed by 40um). For purification of Shh-expressing hair peg cells at E17.5, embryonic back skin was cut into 9 pieces and placed, dermis side down, in dispase (Life Technologies, 0.4mg/ml) dissolved in PBS for 1hr at 37°C. Epidermis containing placodes and hair germs were removed. Dermis samples containing only hair pegs were placed in a 0.25% trypsin solution at 37°C for 10 min. Single-cell suspensions were obtained by triturating the skin gently. Cells were then filtered with strainers (70µm followed by 40µm). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM1977057