title: GSM2940095: Riboseq.recovery.WT_2; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Cells were ground in lysis buffer (20 mM Tris-HCl pH 8.0, 140 mM KCl, 1.5 mM MgCl2, 1% Triton X-100, 0.1 mg/mL cycloheximide) using a cryogenic ball mill. Lysate was digested with 15U/A260 RNase I. Footprinted HeLa lysate was doped in at a fixed fraction to ribosomal RNA per volume of yeast lysate. 80S footprints were isolated from a sucrose gradient, enriched for small RNAs, and size-selected from 15-34 nucleotides. A 3′ adapter was ligated and samples treated with RiboZero. The RNA was reverse transcribed, circularized, and PCR amplified.
attributes.tag: GEO Accession
attributes.value: GSM2940095