title: Rhizosphere.Soil.DNA_Vaccinium.angustifolium_SaguenayField_Plot1_Replicate2
design: 300 mg of sediment samples were used for total genomic DNA extraction using the Nucleospin@ soil kit (Macherey-Nagel). The V3-V4 region of the 16S rDNA, and the fungal ITS rDNA were then amplified in a PCR reaction using the 341F/805R primer pair for the 16S and ITS3_KYO2F/ITS4_KYO3R for the ITS, all of which containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. The amplicons were purified using the Nucleomag@ NGS clean-up and size select kit (Macherey-Nagel). Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.
library_name: S2