title: GSM4142620: cSCC7: cutaneous squamous cell carcinoma biopsy; Homo sapiens; RNA-Seq
library_construction_protocol: 4 mm punch biopsies were collected from 7 unmatched healthy skin and 9 cSCC samples. Snap frozen punch biopsies were homogenized followed by RNA extraction using the miRNeasey mini kit (Qiagen). RNA concentrations were measured using a Nanodrop  and RNA integrity was assessed on the Bioanalyzer. RNA libraries were prepared for sequencing using standard Illumina protocols The library preparation was done using Illumina TruSeq® Stranded Total RNA (with Ribo-Zero Gold) preparation kit. 100 ng of total RNA was depleted of rRNAs using ribo-zero gold magnetic bead based capture-probe system (Illumina Inc.). The remaining RNA (including mRNAs, lincRNAs and other RNA species) was subsequently purified (RNAcleanXP, Beckman Coulter) and fragmented using enzymatic fragmentation. Then first strand synthesis and second strand synthesis were performed and the double stranded cDNA was purified (AMPure XP, Beckman Coulter). The cDNA was end repaired, 3' adenylated and Illumina sequencing adaptors ligated onto the fragments ends, and the library was purified (AMPure XP). The stranded libraries were amplified with PCR and purified (AMPure XP). The libraries size distribution was validated and quality inspected on a Bioanalyzer. Sequencing was performed on NextSeq 500 instrument using v2 reagent kits according to the manufacturer instructions (Illumina Inc.).
attributes.tag: GEO Accession
attributes.value: GSM4142620