title: 16S seq of culture day 1 R1
design: Ten ng of extracted DNA was used as template and the PCR reaction     (25 ?L) contained dNTPs (400nM of each), MgSO4 (1.5 mM), Platinum Taq DNA polymerase     HF (2mU), 1X Platinum High Fidelity buffer (Thermo Fisher Scientific, USA), and     barcoded library adaptors (400 nM) containing V1-3 specific primers: 27F     AGAGTTTGATCCTGGCTCAG and 534R ATTACCGCGGCTGCTGG.
library_name: 16SAMP-13735_S6