title: GSM2695066: WT_10FS2; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from tissues and cell lines using DNeasy Blood & Tissue Kit (Qiagen) as per manufacturer protocols Reduced represenation bisulfite sequencing (RRBS) libraries were prepared according to previously published prototols using gel-free technique (Boyle, P. Genome Biol., 2012).  Briefly, DNA was digested with MspI restriction enzyme, followed by size selection, gap filling and 3'-A-tailing.  Ligation of NEBNext methylated adaptors was performed, followed by bisulfite conversion and index PCR amplificaton.
attributes.tag: GEO Accession
attributes.value: GSM2695066