title: GSM4705626: Microglia_PBS_Control_3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from mouse brain FACS sorted CECs and microglia after Buffer RLT Plus homogenization, and then using QIAGEN RNeasy plus micro Kit. Samples were initially DNAse treated with the GDNAse eliminator column, and finally eluted in in RNAse free water from Qiagen. Isolated RNA sample quality was determined using High Sensitivity RNA Tapestation (Agilent Technologies Inc., California, USA) and concentration was measured using Qubit 2.0 RNA High Sensitivity assay (ThermoFisher, Massachusetts, USA). Libraries were then constructed following manufacturer's instructions for SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA Inc., California, USA) followed by Nextera® XT DNA Library Prep Kit (Illumina, California, USA). Final library quantities were measured by KAPA SYBR® FAST qPCR and quality was assessed using TapeStation D1000 ScreenTape (Agilent Technologies, CA, USA). Resulting final library size was about 430bp with an insert size of about 200bp. Illumina® 8-nt dual-indices were used. Equimolar pooling of libraries was performed basing on QC values and sequencing was performed on an Illumina® HiSeq 4000 (Illumina, California, USA). The read length configuration was 150 PE for 40 M PE reads per sample (20M in each direction).
attributes.tag: GEO Accession
attributes.value: GSM4705626