title: GSM3032935: WT_T_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells or homogenized tissues using TRIzol reagent (Invitrogen) or Direct-zol™ RNA MiniPrep Plus w/ TRI Reagent® (ZYMO Research, R2071) following the manufacturer's protocol. An RNA-seq library was prepared using Novogene's protocol. Briefly, mRNA was enriched using oligo(dT) beads and then fragmented randomly in fragmentation buffer. Next, cDNA was synthesized from these fragments using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) was added along with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand via nick translation. The final cDNA library was sequenced using the Illumina HiSeq platform at Novogene.
attributes.tag: GEO Accession
attributes.value: GSM3032935