title: Foxa1 and Foxa2 are essential for gender dimorphism in liver cancer
design: Foxa1 and Foxa2 are essential for gender dimorphism in liver cancer
library_name: FA2
library_construction_protocol: Briefly, 14-15 day old mice were injected with DEN (20 mg/kg body weight) (Sigma) to initiate tumor growth. Beginning at 28 days, mice were injected with TCPOBOP (3 mg/kg bodyweight) (Sigma) once every two weeks for a total of eight times to promote tumor growth.DEN-induced liver injury experiments were carried out as described previously (Naugler etal., 2007), except that 50 mg/kg body weight Faslodex or estrogen were intraperitoneally injected daily for seven days before DEN treatment. The human samples were not treated. Chromatin was extracted from mouse livers following the protocol described in Li et al. NSMB 2011 [PMID 21623366]. ChIP was performed on the liver chromatin samples. ChIP-DNA was prepared for sequencing with the Illumina GAII sequencer at the Functional Genomics Center at the Institute for Diabetes, Obesity and Metabolismat the University of Pennsylvania.
attributes.tag: Experimental Factor: ORGANISM
attributes.value: Mus musculus
attributes.tag: Experimental Factor: SEX
attributes.value: female
attributes.tag: Experimental Factor: TREATMENT
attributes.value: Untreated
attributes.tag: Experimental Factor: GENOTYPE
attributes.value: Foxa1loxP/loxP;Foxa2loxP/loxP
attributes.tag: Experimental Factor: IMMUNOPRECIPITATE
attributes.value: Foxa2