title: GSM2859373: DO+C CD19+ B cells; Sample_03_CD19; Mus musculus; RNA-Seq
library_construction_protocol: transplantation protocol: HSCs were isolated from young and aged B6.SJL mice. They were cultured for 16 hrs in HBSS + 10% FBS ± 5µM CASIN at 37°C (5% CO2, 3% O2). Subsequently, 600 LT-HSCs were transplanted into sublethally (6,5 Gy) irradiated RAG1-/- mice cell isolation protocol: 12 weeks after transplantation, 50.000 naïve CD4+ T cells and CD19+ B cells were sorted in Ultra Culture medium RNA was extracted from sorted cells using the RNeasy Micro Kit (Quiagen) following manufacturer's protocol cDNA was processed with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). cDNA libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM2859373