title: GSM3876328: MC57G cells, nontargeting siRNA, R443I infection Rep 2 [RNA Polymerase II ChIP seq]; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and PolII-DNA complexes were isolated with antibody. Libraries were prepared using the KAPA Hyper prep Kit (KAPA KK8504). Briefly, 20ng of ChIP'd or input DNA for each sample was subjected to end repair and A-tailing. Illumina adapters were ligated and then the samples were PCR amplified with Illumina primers for 15 cycles. The purified DNA was sequenced on a Hi Seq 4000
attributes.tag: GEO Accession
attributes.value: GSM3876328