title: GSM3638807: 03_OPTC_D00_5030: IIV-Day-0; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from PAXgene tubes using the PAXgene Blood RNA Kit following the manufacturer's protocol. RNA Quality and quantity were assessed by Agilent Tapestation 2200 and Invitrogen Qubit 2.0 fluorometer. 50ng of Total RNA was used to perform a multiplexed gene expression profiling panel of 81 select targets using the TruSeq Targeted RNA Expression guide rev. C following the manufacturer's protocol. In summary, the chemistry begins with reverse transcribing cDNA from purified total RNA. Two custom-designed oligonucleotide probes with adapter sequences hybridize up and downstream of the region of interest. An Extension-ligation reaction, following by amplification creates a new template strand. Templates are then PCR amplified to add indices, creating sequence-ready libraries. Final cDNA libraries quality and quantity were assessed following the previously discussed methods.
attributes.tag: GEO Accession
attributes.value: GSM3638807