title: Illumina sequencing of wPipIV_Harash Wolbachia strain endosymbiont of Culex pipiens
design: Wolbachia being intracellular uncultivable bacteria, a protocol inspired from Ellegaard et al was developed to obtain satisfying quantity of Wolbachia DNA. To do so, Wolbachia enrichment was performed on egg-rafts right after oviposition to maximize the ratio between Wolbachia genomes and Culex genomes. After severals filtrations of a crushed egg-rafts solution to concentrate Wolbachia and removed cell debris, nuclear and mitochondrial genome, a Multiple-displacement amplification (MDA) was carried out directly using the Wolbachia pellet as a matrix, with help of a Repli-g- mini kit (Qiagen)
library_name: wPip_Harash_RSR