title: GSM3036458: reads_EpiKO1a; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from tissues or purified cells using the RNeasy RNA Mini Kit (Qiagen). Complementary DNAs were synthesized using M-MLV Reverse Transcriptase kit (Promega). RT–qPCR was performed using the QuantStudio 3 Real-Time PCR Systems (ThermoFisher Scientific). 18S was used for normalization to quantify relative mRNA expression levels.  Illumina TruSeq stranded and purification of RNA kit was used with 30 ug of total RNA for the construction of sequencing libraries. Libraries were prepared using the strand specific RNA-Seq library preparation TruSeq Stranded mRNA kit (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3036458