title: RNA-seq of Sclerotia aquatilis: adult biological replicate 1
design: mRNA are isolated from total RNA with oligo(dT) method. Then the mRNA are fragmented under certain conditions. Then first strand cDNA and second strand cDNA are synthesized. cDNA fragments are purified, repaired and single nucleotide A (adenine) added. After that, the cDNA fragment are connected with adapters. Those cDNA fragments with suitable size are selected for the PCR amplification.
library_name: Sa-adult01