title: GSM2945749: Wildtype_mESC_HiC_Biological_Rep1; Mus musculus; Hi-C
library_construction_protocol: Hi-C libraries were generated with an in-situ Hi-C protocol (Beleghzal et al, 2016), using the HindIII restriction enzyme.  Briefly, ~25 million cells were crosslinked with 1 % formaldehyde for 10 minutes at room temperature. After quenching the formaldehyde with glycine, the nuclei were isolated, and the DNA was digested with HindIII, end-labelled with biotin-14-dCTP, and in-dilution ligation was performed. Following phenol-chloroform extraction, the biotin was removed from non-ligated ends, and the DNA was sheared by using a Covaris S220 instrument. After A-tailing and adapter ligation, paired-end sequencing was performed on a HiSeq instrument. Hi-C libraries were prepared in two biological replicates from mESC.
attributes.tag: GEO Accession
attributes.value: GSM2945749