title: GSM3034190: RP_MSC_M1_3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested and RNA was extracted using the RNeasy®Mini Kit (Qiagen, 74104). Agilent TapeStation was used for quality measurements. All samples presented RINe values above 9.5. 12 RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacture protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB cat no. E7490). The libraries were mixed into a single tube with equal molarity of all samples. The RNAseq data was generated using HiSeq2500, two lanes, 100 bp single-end read.
attributes.tag: GEO Accession
attributes.value: GSM3034190