title: GSM2940111: DNA methylation_monkey_RS; Macaca mulatta; Bisulfite-Seq
library_construction_protocol: The DNA extraction follows Stem-seq protocol The STEM-seq libraries were generated using the protocol as described previously(Zhang et al., 2018). Briefly, cells were lysed in lysis buffer (10mM Tris-Hcl PH 7.5, 10mM NaCl,0.5% NP-40, protease K (Roche)) for 3h at 55°C and heat-inactivated for 1h at 72°C. Then we added Lamda DNA as a control to calculate bisulfite conversion rate. The DNA mixture was digested by dsDNA fragmentase (NEB) and followed by bisulfite conversion using EpiTect Fast Bisulfite Conversion Kit (Qiagen). Finally, the purified single strand converted DNA is tailed by poly C, followed by extension with a biotinylated poly G containing primer. The extension product is ligated to an adaptor followed by PCR amplification for sequencing library preparation. All libraries were sequenced on Illumina HiSeq XTen according to the manufacturer's instruction. TELP
attributes.tag: GEO Accession
attributes.value: GSM2940111