title: GSM1153763: ZNF; Homo sapiens; OTHER
library_construction_protocol: 50 million exponentially growing cells were harvested and crosslinked with 1% formaldehyde, lysed and cut with the restriction enzyme BglII. After ligation and reversal of the crosslinks, the DNA was purified to obtain the 3C library. This 3C library was further digested with NlaIII and circularized to obtain a 4C library. The inverse PCR primers to make the 4C-seq templates were designed to contain the Illumina adaptor tails, as well as the bait-specific sequences for each of the six loci we interrogated. 4C libraries were multiplexed (three samples per mulitplex), libraries were prepared according to Illumina's instructions for ChIP-seq but without size selection and sequenced on Illumina GAIIxl (76-mer single end tags) Instrument model: Illumina GAIIxl library_strategy: 4C-seq
attributes.tag: GEO Accession
attributes.value: GSM1153763