title: RNA-Seq of sea cucumber: adult male and female circumoral nerve ring
design: Total RNA was extracted from CNR tissue using an RNeasy mini kit with DNase-treatment, following the manufacturers instructions. RNA concentration and quality were determined using an Agilent 2100 bioanalyzer. Total RNA from 3 individuals were pooled for transcriptome construction. The CNR transcriptome was generated following the manufacturers standard procedures. High-quality strand-specific libraries were sequenced on the Illumina HiSeq 2500 platform and 125-bp paired-end reads were generated.
library_name: AJCNS