title: GSM2939000: TP53null-2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed and RNA extracted using the Qiagen RNeasy kit per manufacturer's instructions. Poly-A tailed mRNAs were enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module per manufacturer's instructions. Poly-A tailed mRNAs were then prepped into RNA-Seq libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina. RNA-Seq libraries were amplified and indexed using NEBNext® Multiplex Oligos for Illumina® per manufacturer's instructions. Libraries were sequenced on an Illumina HiSeq 2500.
attributes.tag: GEO Accession
attributes.value: GSM2939000