title: GSM4706143: UV YUMM  1.1-9; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was extracted from MB49, YUMM 1.1 and UV YUMM 1.1-9 cell lines using the QIAsymphony DSP DNA midi kit (937255, Qiagen). Mouse whole exome libraries were constructed using Agilent SureSelect Mouse All Exon kit according to the manufacturer's protocol. The resulting sequencing libraries were analyzed using Agilent D1000 screentape and quantified using KAPA qPCR. Libraries were then normalized and pooled into one pool. The library pool was then clustered and sequenced on the Illumina NextSeq550 instrument using 2x151bp paired end reads to a mean death of 80X, following the manufacturer's protocols. Genomic DNA was extracted from MB49, YUMM 1.1 and UV YUMM 1.1-9 cell lines using the QIAsymphony DSP DNA midi kit (937255, Qiagen). Mouse whole exome libraries were constructed using Agilent SureSelect Mouse All Exon kit according to the manufacturer's protocol. The resulting sequencing libraries were analyzed using Agilent D1000 screentape and quantified using KAPA qPCR. Libraries were then normalized and pooled into one pool. The library pool was then clustered and sequenced on the Illumina NextSeq550 instrument using 2x151bp paired end reads to a mean death of 80X, following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4706143