title: T2C for shSCR in MEL cells rep1
design: T2C was performed in duplicates as described (Kolovos et al., 2014, 2018). In brief, ~10 million cells were isolated, following cross-linking in 1% paraformaldehyde (10 min, 20C; Electron Microscopy Science), chromatin was cut using ApoI or HindIII (New England Biolabs), and ligated in dilution under conditions that allowed most nuclei to remain intact.
library_name: T2C for shSCR in MEL cells rep1