title: RNAseq of Arabidopsis: control
design: Samples were processed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina at ServiceXS (GenomeScan B.V., Leiden, The Netherlands). Briefly, mRNA was isolated from the total RNA suing the oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA was synthesized, ligated with sequencing adapters and amplified by PCR in order to obtain cDNA libraries. Each cDNA library was individually analyzed for quality and yield using a Fragment Analyzer. cDNA was then clustered and a concentration of 1.6 pM was sequenced.
library_name: AtPDA_R3