title: GSM3106136: iPS1 D28 Camk2A+ hpiNs (single cells; SSF896); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the Qiagen RNeasy plus micro kit for the total population RNA seq. For single cell RNAseq (SmartSeq2 library prep), cells were single-cell sorted into 5 μl of 1xTCL buffer (Qiagen,  #1031576). After sorting, plates were immediately sealed, spun down for 5 minutes at 550g and  flash frozen on dry-ice. Sorted cells were then stored at -80°C. Details Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold for bulk RNAseq.For single cell RNAseq, Smart-Seq2 libraries were  prepared by the Broad Technology Labs and sequenced by the Broad Genomics Platform.
attributes.tag: GEO Accession
attributes.value: GSM3106136