title: GSM2936414: SIRT3 KO MEF rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated by a TRIzol (Invitrogen) extraction followed by a Direct-zol RNA MiniPrep Kit (Zymo Research) purification, and 2 μg of total RNA was used for the construction of sequencing libraries. Briefly, 2 mg RNA was incubated with poly-T oligo beads and unbound RNA was washed two times to remove ribosomal RNA and enrich for mRNA transcript pools. The Superscript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA) was used to synthesize cDNA, and cDNA libraries were generated using Nextera XT DNA sample preparation kit. Libraries were sequenced on the Illumina NextSeq.
attributes.tag: GEO Accession
attributes.value: GSM2936414