title: GSM4237847: D120; Mus musculus; RNA-Seq
library_construction_protocol: Colon tumor infiltrating ILCs (Lin-CD45+CD127+) (Lin=CD3e,CD8a,CD19,CD11b,CD11c,Gr1,F4/80,Ter119) from tumors at day 60 (D60) or day 120 (D120) were isolated by flow cytometry and subjected to 3' droplet-based scRNA sequencing. Purity of ILC cells was over 95% for each sample that was determined by post sorting analysis of flow cytometry. Tumor infiltrating ILCs (Lin-CD45+CD127+) (Lin=CD3e,CD8a,CD19,CD11b,CD11c,Gr1,F4/80,Ter119) were isolated and cell suspensions (300-500 living cells per microliter) were loaded on a Chromium Single Cell Controller (10xGenomics) to generate single-cell Gel Bead-In-Emulsions (GEM) by using Single Cell 3' Library and Gel Bead Kit V2 (10xGenomics, 120237). mRNAs of captured cells were barcoded through reverse transcription. Barcoded cDNAs were pooled and cleaned up by using DynaBeads® MyOne Silane Beads (Invitrogen, 37002D). Single-cell RNA-seq cDNA libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10xGenomics, 120237) according to the manufacture's protocol followed by sequencing on an Illumina HiSeq X Ten with pair end 150bp (PE150). 10x genomics scRNA-seq
attributes.tag: GEO Accession
attributes.value: GSM4237847