title: Targeted deep sequencing of HIV-1 2545383_P_day20_POL_RT
design: Viral RNA was extracted from blood plasma from HIV-1 infected individual. Subgenomic area of interest, p51RT(pol) (HXB2 coordinates: 2584-3322) was amplified by RT-nested PCR using 2000 genome equivalent template copies as input. Ion Torrent PGM library was prepared according to manufacturer's instructions.
library_name: 2545383_P_day20_POL_RT