title: Illumina HiSeq 2500 paired end sequencing; Transcriptional differences between the peripheral and the transcription zone of the prostate
design: Transcriptional differences between the peripheral and the transcription zone of the prostate
library_name: TZ_8_p
library_construction_protocol: Patients undergoing radical prostatectomy for organ confined prostate cancer were consented to allow the use of prostate tissue for research via the Biorepository at the Norfolk and Norwich University Hospital (NNUH) under ethical approval granted from the University of East Anglia (FMHS 20122014-37) to comply with the principles expressed in the Declaration of Helsinki. Patients underwent endoscopic extraperitoneal radical prostatectomy. All procedures were conducted by a single surgeon. The prostate gland was removed from the abdominal cavity immediately after resection and rapidly biopsied after extraction to avoid ischaemic artefacts using a standard core biopsy instrument. The extracted whole prostatectomy specimen was placed on a surgical table or equivalent and the subsequent steps were taken to collect the biopsy samples: (1) the apex and base of the gland were identified, the prostate was then cut transversely (axial section) half way along the gland, (2) using the midline and the urethra as guides, biopsies were taken from the peripheral and transition zones avoiding obvious tumour sites where feasible, (3) the remaining prostate gland was sent for histological analysis to inform the malignant status of each core. One tissue core from each zone was placed in RNAlater and stored at -80 °C for downstream RNA sequencing.  Illumina barcoded TruSeq RNA library construction of 18 libraries from 18 total RNA samples
attributes.tag: Experimental Factor: benign
attributes.value: histology
attributes.tag: Experimental Factor: transition zone of prostate
attributes.value: sampling site