title: GSM4453834: C57BL6_Diet 5 (Chol2%_CA)_Liver 1; Mus musculus; RNA-Seq
library_construction_protocol: Liver were harvested, a small portion rapidly snap frozen in liquid nitrogen and stored at -80 ºC. Total liver RNA was extracted using RNAeasy (Qiagen). RNA purity and integrity were confirmed using an Agilent Bioanalyzer. Libraries were prepared from 100 ng total RNA (TruSeq Stranded Total RNA kit, Illumina) and singled-ended sequencing performed on the Illumina HiSeq 2500 using bar-coded multiplexing and a 50 bp read length
attributes.tag: GEO Accession
attributes.value: GSM4453834