title: GSM2942189: ATAC-seq; WT cells; siBRG1; Rep2; Mus musculus; ATAC-seq
library_construction_protocol: 50,000 cells were washed briefly in cold PBS and permeabilized with cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) containing proteinase inhibitor cocktail (Roche). Nuclei were resuspended in 1X TD Buffer (Illumina FC-121-1030), and 2.5uL of Tn5 Transposase (Illumina FC-121-1030) were added. Transposition reaction was performed at 37C for 30 min, and DNA was purified using a Qiagen MinElute Kit. DNA libraries were amplified for a total of 8 cycles. Libraries were assessed for quality control on the BioAnalyzer 2100 (Aglient) to ensure nucleosomal phasing and complexity. Sequencing was performed on the HiSeq 2500 (Illumina), using 50 bp paired-end reads. Sequencing Nextera adpters: F: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG, R: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
attributes.tag: GEO Accession
attributes.value: GSM2942189