title: GSM4512893: HHZ_prophase_I_RNA_rep3; Oryza sativa; RNA-Seq
library_construction_protocol: Libraries for mRNA-seq were constructed using the Smart2-seq method40. Briefly, total RNA was used for reverse transcription and cDNA second strand synthesis using SuperScript II reverse transcriptase (Invitrogen,18064-14) and template-switching oligos. After PCR preamplification and purification with AMPure XP beads (Beckman, A63880), the products were subjected to fragmentation and adaptor ligation using TruePrepTM DNA Library Prep Kit (Vazyme, TD503). PCR amplification was then performed with index-containing primers and 300–800-bp products were purified with AMPure XP beads. Libraries were pair-end sequenced on an Illumina HiSeqX-Ten platform by Annoroad Gene Technology (Beijing). Total RNA was extracted using TRIzol (Takara, 9109). MgCl2 (final concentration: 0.2 M) was added to prevent loss of sRNAs with low GC content. The extracted RNA was stored at -80°Cin LoBind tubes (Eppendorf, 022431021).
attributes.tag: GEO Accession
attributes.value: GSM4512893