title: NextSeq 500 paired end sequencing; Rpd3L HDAC links H3K4me3 to transcriptional repression memory
design: Rpd3L HDAC links H3K4me3 to transcriptional repression memory
library_name: pho23delta-Gal-30_2nd_p
library_construction_protocol: For the time course experiments, the indicated strains were grown in 450ml of SC-raffinose to mid-log phase (OD600 ~0.6) at 22℃. 50ml of cells were collected by centrifugation (raffinose 0). The remaining cells were also recovered by centrifugation, resuspended in SC media lacking any carbon source, and then transferred to media containing the indicated carbon sources. 50ml of cells were collected by centrifugation at the indicated time points.   Total RNAs were isolated with hot acidic phenol/chloroform (1:1) (Sigma).  Sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) after ribosomal RNA was depleted using the Ribo-Zero yeast kit (Epicenter), as recommended by manufacturers.
attributes.tag: Experimental Factor: genotype
attributes.value: pho23delta
attributes.tag: Experimental Factor: growth condition
attributes.value: galactose
attributes.tag: Experimental Factor: time
attributes.value: 30