title: 16S rRNA-seq library of gut of 28-day-old Sus scrofa L.
design: A 466-bp fragment covering the V3 - V4 hypervariable regions of the 16S rRNA gene was amplified using the primers 341F and 806R using Phusion  High-Fidelity PCR Master Mix (New England Biolabs, USA). Sequencing libraries were generated using TruSeq  DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA). The quality of sequencing libraries was assessed using 260/280 ratio of purity in Qubit@ 2.0 Fluorometer (Thermo Scientific, USA) and Agilent Bioanalyzer 2100 system before being sequenced on the HiSeq PE2500 platform (Illumina, San Diego, CA, USA).
library_name: 28-day-old Sus scrofa L. gut metagenome