title: PITX3
design: DNA was isolated from the whole-blood samples. Sanger sequencing was used to identify the mutation. Poly-merase chain reaction (PCR) was performed on the sample by using the primers as follows: F:5 -GTGTCCTGCCCTTATGCCTC-3  and R:5 -GGAGGCTGTGAATCGTTGC-3 . And, PCR products were subjected to DNA direct sequencing.
library_name: CAT-273