title: GSM4199697: M23_SI_12M_F0_5; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using an RNeasy DNA/RNA kit after homogenization and lysis in guanidine thiocyanate buffer supplemented with β-mercaptoethanol. RNA concentration and quality were assessed using an Agilent TapeStation. RNAseq libraries were prepared using an NEB Next RNA Ultra kit with polyA enrichment module using an Agilent Bravo NGS Automated fluidics handling platform as described elsewhere (Misharin et al., 2017, J Exp Med 214). Libraries were multiplexed and sequenced on an Illumina NextSeq 500 platform using 75 cycles of high-output flow cells and a dual indexing strategy. RNA-Seq libraries were prepared using a full-length transcript coverage NEBNext Ultra RNA (New England Biolabs) DNA libraries sequencing was performed using an Illumina NextSeq 500 instrument (Illumina) using 75 cycles of high-output flow cells and a dual indexing strategy.
attributes.tag: GEO Accession
attributes.value: GSM4199697