title: GSM3445846: EUHSR Rapamycin (4h); Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: All cultures were rapidly harvested by vacuum filtration and flash frozen in liquid nitrogen as described in Weinberg et al., 2016 (GSE53268). Frozen pellets were mechanically lysed using a Sample Prep 6870 Freezer/Mill (Spex SamplePrep; 10 cycles of 2 min on, 2 min off at setting 10). Lysate powder was aliquoted and stored at –80°C. Total RNA was extracted using TRI Reagent (Ambion) according to the manufacturer's protocol. rRNA was depleted from 5 μg of total RNA using the Ribo-Zero Gold Yeast rRNA Removal Kit (Illumina) according to the manufacturer's protocol. RNA was randomly fragmented by partial alkaline hydrolysis and then 27- to 40-nucleotide RNA fragments were size-selected and used for library preparation. RNA-seq libraries were prepared as described in Subtelny et al. 2014 (GSE52809) and sequenced on the Illumina HiSeq platform using 40 bp single reads.
attributes.tag: GEO Accession
attributes.value: GSM3445846