title: GSM3444987: TK6_ETO_rep2; Homo sapiens; OTHER
library_construction_protocol: Cells and nuclei were lysed with mild buffers. After blunting of DSBs and BLISS adapter ligation, DNA was extracted using DNA extraction buffer and proteinase K, followed by phenol/chloroform extraction and ethanol precipitation. After DNA sonication, genomic DSB ends were amplified with in vitro transcription, after which generated RNA was subjected to an Illumina small RNA-like library preparation protocol (similar to described for BLISS in Yan, Mirzazadeh et al, Nature Communications 2017).
attributes.tag: GEO Accession
attributes.value: GSM3444987