title: GSM3576359: FM3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed, and RNA was extracted using RNeasy Plus mini kit (Qiagen) 72 hrs post transfection and sent to UCLA TCGB core on dry ice. Ribosomal RNA depletion, and single read library preparation were performed at UCLA core followed by RNA sequencing using NextSeq500.  Coverage was 14 million reads per sample.  FASTQ files with single-ended 75bp reads were then aligned to the human GRCh38 reference genome sequence (Ensembl release 90) with STAR 54, and uniquely-mapped read counts (an average of 14.8 million reads per sample) were obtained with Cufflink 55.  The read counts for each sample were then normalized for the library size to CPM (counts per million reads) with edgeR 56.  Custom R scripts were then used to generate plots.
attributes.tag: GEO Accession
attributes.value: GSM3576359