title: GSM4509271: Control (CTW) rep1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Rat lungs were isolated and frozen in liquid nitrogen and then stored at -80°C after sacrifice.Total RNA was isolated from 50 mg lung tissues using 1 mL Trizol reagent following manufacturer's instructions. RNA integrity and quality were assessed by gel electrophoresis and its concentration and purity were determined by the Thermo Scientific NanoDropTM instruments. Total RNA with high quality was used for cDNA library preparation. Library preparation and RNA-seq were performed on an Illumina HiSeq 2000 platform by Genenergy Biotechnology (Shanghai) Co., Ltd.
attributes.tag: GEO Accession
attributes.value: GSM4509271