title: RNA-Seq of  large yellow croaker dorsal muscle
design: Qualified RNA constituted the sequencing library, which was constructed by means of the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The concentration of the sequencing library was measured on the Qubit 3.0 Fluorometer (Life Technologies, CA, USA) and with the 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). When the effective concentration of sequencing library was more than 10 nM, the sequencing data were generated by Illumina HiSeq 2500 (PE 150bp).
library_name: sample 5