title: GSM3638678: WIII8 2-3hr; Drosophila melanogaster; RNA-Seq
library_construction_protocol: NSUN2-/- female virgins or w1118 virgins were crossed with w1118 males for two days in advance. For each time period, 70-100 embryos were collected to ensure RNA yield and that samples were representative. Total RNA was isolated with TRIzol reagent and Direct-zol RNA MiniPrep kit. For each sample, 1ug total RNA was used for library construction. 1.2μl of 1:50 diluted SIRVs E1 RNA spike-in mix (Spike-in RNA Variant Control Mixes, Lexogen) was added to 1ug total RNA to allow for normalization between the samples. Polyadenylated RNA was separated from total RNA using Oligo dT Magnetic Beads (Vazyme). NEBNext Ultra II Directional RNA Library Prep Kit was used in library construction.
attributes.tag: GEO Accession
attributes.value: GSM3638678