title: GSM3577205: Zika_08 infected; Homo sapiens; RNA-Seq
library_construction_protocol: Brain samples were frozen in RNALater (Ambion®) and stored at -80 ° C until extraction. The tissue was broken and homogenized by TissueRupter® (QIAGEN) and RNA extraction was performed with the RNAeasy Plus (QIAGEN), following the protocol suggested by the manufacturer. Mini® kit The libraries were diluted to 4nM with 15% PhiX. The cDNA library was sequenced with MySeq System (Illumina®, San Diego, CA) using the MiSeq Reagent kit (150 cycles, 2 x 75 paired-end).
attributes.tag: GEO Accession
attributes.value: GSM3577205