title: GSM3874652: embryo_spenKO_f4_4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted according to the manufacturer's recommendations using RNeasy Mini Kit (Qiagen) with on-column DNAse digestion (Qiagen). Only samples showing a RIN score above 9 were used to prepare RNAseq libraries (TruSeq). Libraries were sequenced using NovaSeq 6000 at PE100 settings. cDNA were fragmented to ~200 bp with Focused-ultrasonicator (Covaris, Woburn, MA) and adaptor ligated libraries were generated using NEBNext Ultra DNA library Prep Kit for illumina (New England Labs, Ipswich, MA)
attributes.tag: GEO Accession
attributes.value: GSM3874652