title: GSM4512711: 33dpf_control_2; Danio rerio; RNA-Seq
library_construction_protocol: Single juveniles were cut in small pieces using a razor blade and dissociated with trypsin on ice for 1 hour. mpeg-GFP+ cells were FAC-sorted directly into Trizol reagent. RNA was harvested using Trizol reagent. cDNA amplification: SMART-seq V4 ultra low input RNA kit for sequencing (Takara BIO USA, Inc.). Illumina Truseq Sample Preparation V.2 guide was used and the samples were paired end-sequenced (2x75 bp) on the Illumina HiSeq 2500. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4512711