title: GSM4369115: SS_8Co; Danio rerio; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from pooled zebrafish larvae (30 larvae per sample) for qPCR and RNA-sequencing. Larvae were fixed in Qiazol after which they were disrupted using a tissue disruptor. Subsequently, total RNA was extracted using the miRNeasy mini kit (Qiagen, Venlo, The Netherlands) eluted in 30 µl RNAse free water. Libraries were prepared from total-RNA samples using the NEXTFLEX small RNA-seq Kit  v3. Libraries were checked for size, purity and concentration with a high sensitivity DNA chip on the Agilent Technologies 2100 Bioanalyzer.
attributes.tag: GEO Accession
attributes.value: GSM4369115