title: GSM3036586: Lef1KI_BiolRep2; Mus musculus; RNA-Seq
library_construction_protocol: P1 cells were analyzed and sorted on a FACS Aria II (BD Biosciences) collecting lineage-tagged MECs—GFP+ cells (MECWT) were isolated from ACTA2-CreERT2:ROSA-TG cultures, and GFP– cells (MECLef-1KI) were isolated from ACTA2-CreERT2:Lef-1KI+/+ cultures. Cells were sorted directly into RNA lysis buffer and RNA was extracted using an RNeasy Plus Mini Kit (Qiagen). Samples were treated with DNase and RNA Integrity Numbers (RIN) were assessed using an Agilent BioAnalyzer 2100. All samples had RIN values >10. Indexed cDNA libraries were constructed using a TruSeq mRNA stranded preparation.
attributes.tag: GEO Accession
attributes.value: GSM3036586