title: GSM4199208: Wild Type Gravid Adults sRNA 20C, Rep 3; Caenorhabditis elegans; miRNA-Seq
library_construction_protocol: sample collection: Whole worms were flash frozen in liquid nitrogen prior to Trizol/chloroform extraction Small RNAs (~16-30-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase to facilitate capture of 22G-RNAs. Libraries were then prepared according to the NEBNext® Multiplex Small RNA kit protocol. PCR products (~134-148 bp) were size selected using 10% polyacrylamide gels.
attributes.tag: GEO Accession
attributes.value: GSM4199208