title: GSM3504375: Control CM nucRNA-seq rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA from Bead-bound PCM1+ nuclei was collected using the RNEasy Plus Micro kit (Qiagen). Nuclear RNA-seq libraries were constructed with the Stranded RNA-seq Kit with Ribo Erase (Kapa Biosystems) with custom Y-shaped adapters. Paired-end 2x75 bp sequencing was performed for RNA-seq libraries on an Illumina Nextseq instrument (DNA Link). Reads were first mapped to the mouse genome (mm10) using STAR. Differential expression analysis was then carried out with DESeq2.
attributes.tag: GEO Accession
attributes.value: GSM3504375