title: GSM2356899: S2-KECSTclone8; Homo sapiens; RNA-Seq
library_construction_protocol: Spheres were harvested and washed with PBS twice and the cell pellets were snap frozen in liquid nitrogen. Total RNA was prepared with PicoPure RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. RNA-Seq libraries were built using KAPA Stranded mRNA-Seq kit (KK8400, KAPA Biosystems, Wilmington, MA) according to the manufacturer’s recommendation. Barcoded libraries were multiplexed and sequenced as single-end 75bp reads on Illumina NextSeq 500 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM2356899