title: GSM3400672: Maf fl/fl Il7rCre ATAC rep 1; Mus musculus; ATAC-seq
library_construction_protocol: 15 to 17 thousand CD4- CD8- TCRgd+ CD3e+ CD24+ CD45RBlo thymocytes were sort purified from pooled E17-E18 Maf+/+ Il7rCre and Maf fl/fl Il7rCre fetuses. The Omni-ATAC protocol was performed according to Corces et al (Nature Methods, 2017). Nuclei were subjected to Tn5 transposition (Illumina) and transposed DNA was purified using a Qiagen MinElute Kit prior to library amplification. Transposed DNA fragments were amplified with Nextera PCR primers for a total of 9-11 cycles using NEBNext High-Fidelity Polymerase (NEB).
attributes.tag: GEO Accession
attributes.value: GSM3400672