title: EV-E full genome
design: We used 20 ul of single strand cDNA to prepare second strand cDNA using the TruSeq RNA LT Sample Preparation Kit (Illumina) followed by library preparation according to the manufacturers instructions. The concentration of dscDNA or DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). The average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The libraries were diluted to 7.0pM and sequenced paired end for 300 cycles using the MiSeq system (Illumina).
library_name: 1_S1_L001