title: GSM4515955: mRNA-Seq, mutant 3; Mus musculus; RNA-Seq
library_construction_protocol: GFP+ cells from pharyngeal arch 3-6 of mouse embryos at E10.5 were isolated using FACS Aria II sorter. the quality of the total RNA was assessed using the Agilent 2100 Bioanalyzer.  The RNA Integrity Number (RIN) of all of our samples was 9.5 or above. The SureSelect Strand Specific mRNA library kit was used for library construction, as per the manufacturer's instructions (Agilent). Library construction began with ribosome reduction using the NEBNext rRNA depletion kit. The resulting RNA was randomly fragmented with cations and heat, which was followed by first strand synthesis using random primers with inclusion of Actinomycin D (2.4ng/µL final concentration) and second strand cDNA production.
attributes.tag: GEO Accession
attributes.value: GSM4515955