title: Bronchial biopsy from SIB004
design: BAL cells and bronchial biopsies were immediately placed in RNAlater and stored frozen until RNA extraction was performed. Total RNA from BAL cells and bronchial biopsies was extracted using the RNeasy Mini kit (Qiagen) and RNA integrity was confirmed using the Agilent 2100 BioAnalyzer (Agilent, Palo Alto, CA). Samples were required to have a RIN of at least 7.0.  Sequencing libraries were prepared from RNA from each sample using the Illumina TruSeq Stranded RNA HT sample preparation kit with RiboZeroTMGold.  Libraries were barcoded and then sequenced using an Illumina HiSeq 2000 with 51 bp paired-end reads.
library_name: Biopsy_SIB004