title: GSM3400610: BAP1 WT; Mus musculus; RNA-Seq
library_construction_protocol: Epidermal cells from neonates of the same genotype were pooled (Rosa26-CreERT2, n=11; Bap1fl/fl Rosa26-CreERT2, n=9) and cultured in medium M254 (Thermo Fisher Scientific) containing 1× human melanocyte growth supplement (HMGS, Thermo Fisher Scientific) with 100 nM 4-OHT for 4 days.  After 3 more days of culture without 4-OHT, cell suspensions were prepared with Accutase (Sigma) and approximately 6,000 viable cells processed for single-cell RNA-seq with the Chromium Single Cell 3' Library and Gel bead kit v2 (10x Genomics). cDNAs and libraries were prepared according to manufacturer instructions (10× Genomics)
attributes.tag: GEO Accession
attributes.value: GSM3400610