title: GSM2859321: ZF_WT_2; Danio rerio; RNA-Seq
library_construction_protocol: For next generation sequencing (NGS) RNA was isolated using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and libraries integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). 500 ng total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry with 2x75bp paired end setup.
attributes.tag: GEO Accession
attributes.value: GSM2859321