title: GSM3502868: VGluT2_3_MeDIP; Mus musculus; MeDIP-Seq
library_construction_protocol: Fixed nuclei for FACS using mKate2 fluorescence DNA was isolated from FACS sorted nuclei and sheared using Bioruptor plus (Diagenode # B01020001) sonication device and purified using SureClean plus (Bioline, #BIO-37048) as described in (Halder et al.,2015). 100ng of DNA samples were end-repaired and ligated with NEB adapters (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and cleaned up using Agencourt AMPure XP (Beckman Coulter, #A63881). MeDIP reaction was performed using Methylated DNA IP kit (Zymo Research #D5101) according to manufacturer protocol.
attributes.tag: GEO Accession
attributes.value: GSM3502868