title: Metagenome of soil from Grassland: Paleudalf soil
design: Twenty nanograms of purified DNA was used as a PCR template with KlenTaq  LA DNA Polymerase, which targeted the V4 region (515-806 position) of the bacterial 16S rRNA gene. The quality was verified by agarose electrophoresis and an amplicon quantification was performed with Qubit  4 Fluorometer. Amplicon pools were quantified for sequencing by qPCR with a Kapa NGS quantification kit. The sequencing of the amplicon libraries was performed with a MiSeq  Reagent kit v2 , generating pair-end reads.
library_name: GPf