title: Illumina Genome Analyzer II sequencing; Transcriptome profiling of Arabidopsis mutants of the chlorophyll degradation PAO/Phyllobilin pathway
design: Transcriptome profiling of Arabidopsis mutants of the chlorophyll degradation PAO/Phyllobilin pathway
library_name: nye1_2dDD_1_s
library_construction_protocol: Entire leaf n°6 of Arabidopsis, 25 days after germination, grown in short day condition (6h light, 18h dark) were harvested, detached and put in the dark for up to 2 days. 0d and 2d samples have been harvested in triplicate for each condition. On soil. 6 h light, 18h dark, 100umol/m2 light, 60% humidity.  RNA was extracted using Qiagen RNeays kit with a DNAse removal step. Quality of RNA was controlled by Bioanalyzer.  Truseq Stranded Total RNAseq kit from Illumina.
attributes.tag: Experimental Factor: genotype
attributes.value: nye1 mutant
attributes.tag: Experimental Factor: environmental stress
attributes.value: continuous dark (no light) regimen
attributes.tag: Experimental Factor: time
attributes.value: 2