title: GSM3103289: G1_44; Rattus norvegicus; RNA-Seq
library_construction_protocol: Immediately after the probe trial on day 3 of testing, animals were anesthetized with isofluorane gas and decapitated using a guillotine, and brains were rapidly dissected and frozen. Dissected tissues were stored in pre-weighed microcentrifuge tubes at -70°C until analysis. Dissection of the CA1/CA2 region of the hippocampus (plates 39-42) was according to plate designations in Paxinos and Watson (1998). The brain was cut in the coronal plane to obtain access to the ventral CA1/CA2 region of the hippocampus. The dentate gyrus and the alveus were excluded. RNA was isolated from the left hippocampal region of each animal using TRIzol® Reagent (Invitrogen) and then purified with the RNeasy Kit (Qiagen) using a modified version of the RNeasy Trizol procedure. Rather than precipitating with 0.5mL isopropanol per 1mL Trizol, 1 volume of 70% ethanol was used. After precipitation, the RNeasy protocol was used. However, rather than using 700ul of RW1, half the volume was used, and DNase was used after this step. After the DNase step, another 350ul of RW1 was used, and the rest of the protocol was followed as originally written. Each sample was prepared for sequencing using the TruSeq RNA Sample Preparation Kit (Illumina Inc), following the manufacturers protocol.
attributes.tag: GEO Accession
attributes.value: GSM3103289