title: Liver transcriptome profile in pigs with extreme phenotypes of intramuscular fatty acid composition
design: From the 10 selected animals, total RNA was isolated from liver using the RiboPureTM Isolation of High Quality Total RNA (Ambion*, Austin, TX) following the manufacturer’s recommendations. RNA was quantified using the NanoDrop ND-1000 spectrophotometer (NanoDrop products, Wilmington, USA) and checked for purity and integrity in a Bioanalyzer-2100 (Agilent Technologies, Inc., Santa Clara CA, USA). Sequencing libraries were generated using Illumina mRNA-Seq following manufacturer’s instructions and ten index codes were added to attribute sequences to each animal. A total of two channels of an Illumina Hi-Seq 2000 instrument (Fasteris SA, Plan-les-Ouates, Switzerland) were used to sequence two pools of five samples (one pool with five samples per lane with barcoding).
library_name: GPF-6