title: RNASeq of MEC-1 cell line
design: Total RNA was purified using High Pure RNA Isolation kit (Roche). The quality and quantity of isolated total RNA was assessed using the Agilent 4200 TapeStation system (Agilent Technologies) and the Qubit RNA HS Assay Kit (Invitrogen). Sample had an RNA integrity number (RIN) of more than 9.0. 150 ng of total RNA was subjected to mRNA enrichment using the NEBNext poly(A) mRNA Magnetic Isolation Module prior mRNA library preparation using the NEBNext Ultra Directional RNA library Perp Kit for Illumina (both NEB). Prior sequencing on the in-house NextSeq platform (Illumina), library was qualified and quantified using the Agilent 4200 TapeStation system (Agilent Technologies) and the Qubit dsDNA HS Assay Kits (Invitrogen)
library_name: Mec1_wt_1_RNASeq