title: RNA-Seq of Acipenser gueldenstaedtii: adult male testes, pool of 4
design: Total RNA was extracted from each sample using TRI Reagent (Sigma). The concentration and integrity of RNA were examined using a Thermo-Fisher Scientific NanoDrop 8000 Spectrophotometer and Agilent 2100 Bioanalyzer. All RNA samples had OD260/280 1.8 and RNA integrity number (RIN) 7. Equal amounts of the RNA samples from each group were then pooled together for cDNA synthesis and sequencing. cDNA libraries were prepared with ~2.5 g of total RNA using NEBNext Ultra RNA library prep kit (New England Biolabs). The libraries were sequenced with one lane on an Illumina NextSeq 500 instrument with 75-bp single-end reads.
library_name: TestesM1_5_S13_R1_001