title: GSM4018791: TH6729_16 min; Streptococcus pneumoniae; RNA-Seq
library_construction_protocol: Total RNAs were extracted with the TIANGEN RNA extraction kit (TIANGEN, Beijing, China) based on the supplier's instructions, treated with RNase-free DNase I (New England Biolabs, Beijing, China), and final purified with QIAGEN RNA purification kit (QIAGEN, Beijing, China) according to manufacturer's protocol. Sample 1-6: The ribosomal RNA was removed from total RNA (~2 µg) using RiboZeroTM Magnetic Kit (EpiCentre, Cleveland, OH).  The processed RNA samples were used to prepare double-stranded complementary DNA (cDNA) libraries with the M-MuLV Reverse Transcriptase and NEBNext® mRNA Second Strand Synthesis Module.  The cDNA molecules were fragmented by sonication, and further processed for end repair, adaptor attachment, and sequence amplification. Sample 7-18: The ribosomal RNA was removed from total RNA (~1.5 µg) using poly-T oligo-attached magnetic beads. Fragmentation of mRNAs and sequencing libraries were generated using NEBNext® UltraTM RNA  Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. The cDNA molecules were ligated with the NEBNext Adaptor, and further processed for selected, purifed, and sequence amplification.
attributes.tag: GEO Accession
attributes.value: GSM4018791