title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of human breast cancer cell line MCF-7 treated for 24h with rapamycin or DMSO
design: RNA-seq of human breast cancer cell line MCF-7 treated for 24h with rapamycin or DMSO
library_name: Sample 2_p
library_construction_protocol: Samples were collected after 24h treatment with vehicle or rapamycin. Total RNAs were isolated using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's recommendations.  Libraries were made using 1 μg of total RNA (three replicates per condition) and the TruSeq Stranded mRNA Library Preparation Kit (Illumina, Paris, France) according to manufacturer's recommendations
attributes.tag: Experimental Factor: genotype
attributes.value: pLX-empty
attributes.tag: Experimental Factor: compound
attributes.value: dimethyl sulfoxide