title: GSM2938806: smallRNA seq data from healthy control N13; Homo sapiens; ncRNA-Seq
library_construction_protocol: Serum samples were collected in 10-mL BD serum tubes and centrifuged at 4°C for 20 minutes at 3000 rpm. The supernatant serum was then aliquoted and total RNA containing miRNA was extracted from the samples using the Serum miRNA purification kit (Genolution, Seoul, Korea) according to the manufacturer's instructions. The libraries were prepared for 50bp single-end sequencing using the NEXTflex Small RNA-Seq Kit (Bioo Scientific Corp.). Namely, smallRNA molecules were isolated from 1μg of total RNA via the adapter ligation. The isolated smallRNAs were synthesized as single-stranded cDNAs through the RT (Reverse transcription) priming. By applying this as a template for second strand synthesis, double-stranded cDNA was prepared through PCR (Polymerase Chain Reaction). And, the fragments of ~150bp were extracted for sequencing through size selection by gel electrophoresis. Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA) followed by quantification with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturer's protocol. Following cluster amplification of denatured templates, sequencing was progressed as single-end (50bp) using Illumina HiSeq2500 (Illumina, CA, USA).
attributes.tag: GEO Accession
attributes.value: GSM2938806