title: DNA-Seq of soil bacteria
design: For each sample, genomic DNA was extracted from 0.25 g fresh soils with a PowerSoil DNA Isolation Kit (MoBio Laboratories, Inc., Carlsbad, CA, USA) following the manufacturers instruction. An approximately 410 bp region of the 16S rDNA gene was amplified with bacterial specific forward primer 515F and reverse primer 909R (Baker et al., 2003). Primers 515F contained a unique 12 nt barcode at the 5end for Miseq sequencing detection.
library_name: bA1