Genomic screening of Saxifraga granulata to develop of microsatellite markers
The DNA was prepared for an Illumina Paired-End (IPE) shotgun library according the manufacturer’s guidelines (Illumina, San Diego, USA) and run on a HiSeq 2000 system. The reads were imported in PAL Finder v.0.02.04 software (Castoe et al., 2012) to extract simple sequence repeats (SSRs) and to develop primer pairs for amplification.