GSM2790176: pSCR_FLAG-AGO1-IP; Arabidopsis thaliana; ncRNA-Seq
For each FLAG-AGO1 allele, ~5 different plates were frozen and ground in liquid nitrogen. Supernatants were precleared and incubated with EZview Red ANTI-FLAG M2 affinity Gel (Sigma). The beads were removed by spinning and RNA was then extrafcted using Trizol reagent (Life Technologies). Extracted RNA was processed into sequencing libraries using adapted Illumina protocols and sequenced at Fasteris (http://www.fasteris.com, Switzerland) using the Illumina HiSeq sequencer