mRNAseq 18°C #1 (replicate b)
Total RNA was extracted from pooled ovaries with TRI Reagent (Ambion) according to the manufacturer’s instructions. RNA concentration and purity of the probes was tested as describes above. mRNA libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina) and final DNA concentration of the libraries was tested as described above. Four libraries (two probes, two replicates each) were sequenced on an Illumina HiSeq 4000 system (paired end, read length=100).