Whole exome sequencig of homo sapiens: Primary tumor Pancreas
500ng of genomic DNA was fragmented to a target size of 150 to 200 bp on the Covaris LE220 sys-tem. Barcoded libraries (Kapa Biosystems) were subjected to exon capture by hybridization using the SureSelect Human All Exon 51MB V3 or V4 kit (Agilent).