GSM2299973: AB_blocked; Drosophila melanogaster; miRNA-Seq
RNA enriched for fragments <200nt was extracted from 50 pooled male abdomens, using the miRvana miRNA kit (Ambion). The RNA sample was split and subjected to two different library construction protocols - one using a blocking oligo complentary to a contaminating 30mer rRNA sequence, and one without the blocking oligo. HD sRNA-seq libraries, created using HiSeq2500 IL adapter; library sequenced by BaseClear provider.