GSM4582560: oropharyngeal endoderm explant stage 39 rep4 [39_G]; Ambystoma mexicanum; RNA-Seq
Replicates of oropharyngeal explants at different stages of development (37/38, 39, 41) were immersed in RNALater.  Samples were homogenized with a Dounce homogenizer in RLT buffer containing ß-mercaptoethanol (Qiagen).  The homogenate was then processed using an RNAEasy kit (Qiagen, Hilden, Germany). Replicate samples for each stage were analyzed for RNA quantity and quality on a Nanodrop 8000 spectrophotometer. RNA libraries were prepared for sequencing using standard Illumina protocols