GSM3963720: FG_2; Platichthys stellatus; RNA-Seq
Three samples for each tissue were used for the following experiment. A total of 24 samples named MB1-3, ML1-3, MG1-3, MM1-3, FB1-3, FL1-3, FG1-3, and FM1-3 were acquired and their total RNAs were extracted via Trizol reagent (Invitrogen, USA) and measured with the Agilent 2100 RNA 6000 Nano kit (Agilent, USA) for quality control. Twenty-four cDNA libraries were constructed using 3 μg RNA (RIN > 7.0) from each sample via the conventional protocol, and the subsequent sequencing was conducted with an Illumina HiseqTM 4000 by Novogene Co. Ltd (Beijing, China). RNA libraries were prepared for sequencing using standard Illumina protocols