GSM2298977: nat4D; Saccharomyces cerevisiae; RNA-Seq
Total RNA from logarithmically grown (O.D. 0.8) yeast cells was isolated using the hot phenol extraction method [Schmitt ME et al., Nucleic Acids Res 1990] and then treated with TURBO DNA-free DNase kit (Ambion, AM1907). cDNA libraries were prepared from 10μg of total RNA using the Illumina mRNA-Seq-Sample Prep Kit according to manufacturer's instructions. cDNA fragments of ~400 bp were purified from each library and confirmed for quality by Agilent 2100 Bioanalyzer.