16s rRNA sequencing of Shark microbiomes
Purified DNA was amplified using PCR (Polymerase Chain Reaction) and                 primers R806 and F515 targeting the V4 region of the 16S rRNA gene.Amplicons were                 sequenced with an Illumina MiSeq sequencing platform equipped with a V2 chemistry                 500 cycle cartridge. Initial processing of sequence data was performed in MacQIIME                 (Quantitative Insights into Microbial Ecology) version 1.9.1