16S amplicon sequencing, prairie 2010
Using environmental DNA, the V6-V8 variable region of the 16S SSU was amplified using primers and a PCR protocol described in de Oliveira et al. (Veterinary Microbiology, 2013). Libraries were constructed using Lib-L kits, with 0.8 DNA molecules per bead. We pooled 15 samples per sequencing run, distinguishing amongst them with 5 bp barcodes.