GSM1463460: VCaP_AR_siPIAS1_R1881_rep2; Homo sapiens; ChIP-Seq
Lysates were derived from sonicated nuclei and AR-, PIAS1-, FOXA1-, Pol2 and H3K4me2-DNA complexes were isolated with 1 mg (one microgram) of antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland, BGI Tech Solutions, Shenzhen, China or at the Gene Core in EMBL, Heidelberg, Germany.