Carboxylate biofuel platform post fermentation samples of superior performance
Twenty samples of bacterial communities that exhibited superior fermentation performance on the carboxylate biofuel platform. Sequencing was performed by Research and Testing Laboratories (Lubbock, Texas) for bacterial tag-encoded FLX amplicon pyrosequencing (BTEFAP) on a Roche Life Sciences 454 FLX sequencer (Roche Applied Science, Werk Penzberg, Germany) with titanium chemistry. Bacterial sequences were amplified with the following primer set: forward and tagged 27F 5'-GAG TTT GAT CNT GGC TCA G-3', and reverse 519r 5'-GTN TTA CNG CGG CKG CTG-3'.