GSM3772853: C6_SK_PHA_III_2D; Homo sapiens; RNA-Seq
RNA was isolated with RNeasy Plus Mini Kit as described by manufacturer (QIAGEN, Cat. 74136). Transcriptome RNA-Sequencing (RNA-Seq) was performed using Illumina high-throughput RNA sequencing. DNA libraries were prepared using TruSeq Stranded mRNA Library Prep Kit. The DNA libraries were sequenced according to the Illumina TruSeq v3 protocol on an Illumina HiSeq2500 sequencer. Paired-end reads were generated of 100 base-pairs in length. Alignment was performed using the HiSat2 aligner (version 2.2.0.4) against the human GRCh38 reference genome. Gene expression was quantified using the FeatureCounts software (version 1.4.6) based on the ENSEMBL gene annotation for GRCH38 (release 84).