GSM2703818: BEC2; Gallus gallus; RNA-Seq
Total RNAs were isolated from the eyelid tissues of the six chickens with TRIzol® Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacture’s protocol. The extracted RNA (3 μg per sample) was used to construct a transcriptome library with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Beverly, MA, USA), according to the manufacturer’s protocol. Different index codes were added to each sample library. The libraries were constructed in a series of steps, including cDNA synthesis, blunt ending, addition of adaptors, selection of cDNA fragments, PCR, and purification of the PCR products.