Microbial Diversity of   soil
For PCR amplification, the primer 338F and 806R were used to amplify the variable regions V3 and V4 of the bacterial 16S rRNA gene (Fang et al., 2018).The resulted PCR products were extracted from a 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantiFluor-ST (Promega, USA) according to the manufacturers protocol. The PCR Products after purifying were submitted to the Majorbio Co.,Ltd. (Shanghai, China) for paired-end sequencing on the Illumina MiSeqplatform.