GSM2521581: vehicle 2; Mus musculus; RNA-Seq
Total RNA was isolated from ~30mg muscle tissue (quadriceps muscles from treated and control DBA/2J-mdx male 6 month-old mice) with the RNeasy Protect Mini Kit as per manufacturer’s instructions. RNA was quantitated at the Qubit fluorometer and quality-controlled at a 2100 Bioanalyzer. Libraries were prepared from approximately 1ug RNA/sample by means of TruSeq Stranded Total RNA Library Prep Kit. Libraries were sequenced through the Illumina NextSeq 500 System (high-throughput, paired-end 150bp fragment sequencing.