GSM2254246: Sall4 ko SF oocyte, replicate 1 [RNA-seq]; Mus musculus; RNA-Seq
secondary and early antral follicle oocytes were obtained from Sall4fl/fl;Zp3-Cre (Sall4 KO) and Sall4fl/fl ovaries of P12.5 and P17.5 female mice. In the microinjection experiments, we detached secondary follicles from P10.5 WT female mice ovaries. Reverse transcription was performed directly on the cytoplasmic lysate. Terminal deoxynucleotidyl transferase was then used to add a poly(A) tail to the 3’ end of the first-strand cDNAs. The total cDNA library of the single cell was then amplified by 18- 20 cycles for the library construction. The amplified cDNA was fragmented using Covaris sonicator (Covaris S220). To generate the sequence libraries, the TruSeq® Library Prep Pooling kit (Illumina 15042173) or NEBNext Ultra DNA Library Prep Kit (New England Biolabs E7370) was used following the manufacturer’s instructions.