GSM2299071: ChIP-Seq analysis of Ser2-Phosphorylated_PolII in mouse ESCs under shPhf5a knockdown treatment replicate 3; Mus musculus; ChIP-Seq
Ten million cells were used for the ChIP and precipitated using 10 micrograms of antibody  against Ser-5-Phosphoryalted RNA polymerase II (Abcam, ab5131 ) and  against Ser-2-Phosphoryalted RNA polymerase II (Active Motif, clone 3E10) Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.