ITS rRNA gene sequencing of Avicennia germinans leaves
Fungal DNA was amplified at the ITS 1F-2 rRNA gene with modified versions of the ITS1F and ITS2 primer set. PCR products were then pooled to equimolar concentrations and amplicon sequencing was performed using paired-end reads with an Illumina v3 (2 x 300 bp) cartridge on an Illumina MiSeq.