GSM1463457: ES; Mus musculus; Bisulfite-Seq
Genomic DNA was isolation ES and Neuro2A cell stock using phenol-chloroform extraction protocol, precipitated using ethanol precipitation protocol and resuspended in 1X TE buffer. In brief, genomic DNA isolated from ES and Neuro2A cells were fractionated by sonication, ligated to an adaptor and modified with bisulfite conversion protocol. It was then amplified with a PCR scheme designed to enrich IAP LTR-genomic regions, and finally size-fractionated with agarose gel electrophoresis. The prepared libraries were sequenced with a Next-Generation-Sequencing protocol. [For somatic cell protocol, see "protocol" field under SAMPLES.]