GSM2790200: Col0_pWER_HF-RPL18-IP Rep2; Arabidopsis thaliana; RNA-Seq
For each sample, ~20 different plates were frozen and ground in liquid nitrogen. Supernatants were precleared and incubated with EZview Red ANTI-FLAG M2 affinity Gel (Sigma). The beads were removed by spinning and RNA was then extracted using phenol:chloroform. Extracted RNA was processed into sequencing libraries and sequenced at Fasteris (http://www.fasteris.com, Switzerland) using the Illumina MiSeq sequencer