GSM3772912: A549 normoxia rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using the miRNeasy Mini kit (Qiagen), including the optional on-column DNA digestion with the RNase-Free DNase Set (Qiagen). After isolation, 500 ng RNA were quality checked on a 1% agarose gel. rRNA was depleted using the RiboZero kit (Zymo). Libraries were prepared and sequenced on a Illumina NextSeq sequencer with HighOutput