GSM2610674: Wild-type Villus ATAC-seq biological rep2; Mus musculus; OTHER
Nuclear prep from villus cells were used for transposition reaction using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.