GSM4647534: H3K27ac R586W clone 2 rep 2; Mus musculus; ChIP-Seq
Cells were crosslinked in 1% formaldehyde for 5 minutes, and lysed using sequential treatment with hypotonic and detergent-containing buffers. Chromatin was sheared to 200-1000bp using a Covaris sonicator. Kapa Hyper Prep library kit was used according to manufacturer's instructions.