GSM2702121: 10-inp-mice-breast_S4; Homo sapiens; ChIP-Seq
Tumors were frozen in liquid nitrogen. For ChIP the detailed protocol from Scmidt D. et al (Methods 2009) were followed, ie samples were pulverised and x-linked in PBS with 0,5% folmaldehyde for 10 minutes, then proceeded with Lysis buffers. Library construction: ThruPLEX (Rubicon Genomics, Ann Arbor, MI, USA) supplied by Diagenode. The manufacturer’s instructions were followed.