GSM3965408: AGA HF rep 2; Sus scrofa; RNA-Seq
Tissue (~50 mg) was homogenized in 1 mL TRIzol Reagent (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer's protocol steps 1-8. After step 8, 0.4 mL of 200 proof ethanol was added and the samples briefly vortexed. The samples were loaded into RNeasy Mini Kit columns (cat. No. 74104, Qiagen, Germantown, MD, USA) and the manufacturer's protocol followed from Part 1 step 3. Genomic DNA was removed with the RNase-free DNase Set (cat. No. 79254, Qiagen, Germantown, MD, USA) following manufacturer's protocol. Libraries were constructed and sequenced using the TruSeq LT Stranded RNA Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA) was used with 1 ug of total RNA for the construction of sequencing libraries