GSM3405535: AC8926; Homo sapiens; RNA-Seq
Skin lesions were biopsied, stabilized in RNA-later and frozen at 80ºC . Total RNA was isolated from fresh tissue with Trizol (Invitrogen, USA) followed by purification with the RNeasy mini kit (Qiagen, USA). RNASeq libraries were prepared using the TruSeq®Stranded mRNA LT Sample Prep Kit (Illumina, USA). RNA libraries were prepared for sequencing using standard Illumina protocols