Mate pair sequencing of genomic DNA samples from donor norm1 (control sample).
Mate pair sequencing was conducted to detect the genomic breakpoint around the Shank3 gene in PDMS patients. Genomic DNA samples isolated from 2 PDMS patients and 2 healthy individuals were used to construct meta-pair sequencing libraries using the Illumina Nextera Meta Pair Library Prep Kit according to the manufacturer’s instructions. Libraries were sequenced to 76x2 paired-end reads on an Illumina HiSeq2500. Reads mapping to Chromosome 22 around the Shank3 gene are selected.