TFIIIC ChIP-Seq in H1 hESCs
H1 hESCs were grown on Matrigel using mTESR for 5 days, cross-linked with 1% formaldehyde for 15 mins and harvested. Antibody against human TFIIIC subunit TFIIIC63 (Cat# A301-242A Bethyl Antibodies) was used to perform ChIPs (as described in Oler et al., NSMB 2010). Eluates from 3 ChIPs were pooled and library preparation was carried out per Illumina's guidelines (using the TruSeq library prep kit).