GSM4456054: osteoblast D3; Homo sapiens; RNA-Seq
For constructing the library, 5 μg of RNA for each sample was prepared. Epicentre Ribo-Zero™ rRNA Removal Kit (Epicentre, USA) was used to remove rRNA, and ethanol precipitation was applied to clean up rRNA-free residue. In the library construction of mRNAs, the linear RNA was treated by RNase R (Epicentre, USA), which was not used in the library construction of circRNAs. The sequencing libraries were generated by using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA) according to manufacturer's protocol.