Pooled RNA sample  for  RNAseq  library
Pooled RNA sample for RNAseq library (pool equimolar, Q­‐bit quantified quantities of five RNA samples extracted from seedling and one RNA sample extracted from xylem).  RNA sample volume: 100 ul.  Total amount of RNA estimated:32,000 ng. Added 2.5 Volumes (250 uL) of absolute ethanol to the 100 ul of RNA Sample.  NO NaAc added.  Sequencing: this library was barcoded and sequenced into one Hiseq2500 lane, PE 2x150, TruSeq SBS, pooled with other library.