RNA virome from a water sample collected from Lake Limnopolar (Byers Peninsula, Livingston Island, Antarctica) in November 2006
RNA viruses were purified by tangential flow filtration (0.45 um), sucrose cushion (25%) and nucleases treatment. Protected RNA genomes were extracted by Trizol followed by a treatment with DNase I RNAse free and then ramdomly amplified by Sequence Independent Single Primer Amplification.