P6 metabarcoding from soil in TLT15-15 library constucted using hard gridding strategy
DNA metabarcode library constructed a using trnL P6 loop DNA barcode (Taberlet et al, 2007) amplified from TLT15-15 soil, in the Salar the Atacama Basin, Antofagasta, Chile. For this library a hard gridding strategy was use to extract DNA from soil, where the soil was agitated four times in in FastPrep-24 Instrument (MP Biomedicals, EEUU), aiming aiming DNA from seeds and wooden tissues . Details in Carrasco-Puga et al (unplublished)