GSM4296922: TEAD4 knock Down HTS cell (replicate 1); Homo sapiens; RNA-Seq
Total RNA from the HTS cells and TEAD4 knock down HTS cells were isolated using RNeasy Mini Kit (#74104) from Qiagen per the manufacturer's protocol with on column DNase digestion.  RNA concentrations were quantified using a NanoDrop Spectrophotometer at a  wavelength of 260nm. Integrity of the total RNA samples was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA). The total RNA fraction was processed by oligo dT bead capture of mRNA, fragmentation, reverse transcription into cDNA, end repair of cDNA, ligation with the appropriate Unique Dual Index (UDI) adaptors, strand selection and library amplification by PCR using the Universal Plus mRNA-seq +UDI library preparation kit (NuGEN 0508-08, 0508-32).