Pol III ChIP-seq in ADS_iPS cells (iPS cells derived from ADSCs)
ADS-iPSCs (from Dr.Ron Evans' lab at Salk Institute) were grown on Matrigel in mTESR for 5 days, cross-linked with 1% formaldehyde for 15 mins and harvested. Antibody against human Pol III subunit Rpc155 (from Dr.Bob White's lab) was used to perform ChIPs (as described in Oler et al., NSMB 2010). Eluates from 3 ChIPs were pooled and library preparation was carried out per Illumina's guidelines (using the TruSeq library prep kit).