RRBS of Ovis Aries: Pubertal Semen Treatment Ram 5, Litter 10
For the RRBS libraries we need at least 100ng of gDNA, we digest it with MspI and treat it with bisulfite. The individually barcoded libraries are then pooled and sequenced on the HiSeq 4000 with single-reads 100nt. Each lane produces ~ 280-300 million reads