GSM1464522: Sf_BV_24; Spodoptera frugiperda; miRNA-Seq
Total RNA was extracted from cells using Tri-reagent  according to the manufacturer’s instructions (Molecular Research  Center). RNA concentrations were measured using a spectrophotometer,  and integrity was ensured through analysis of RNA on a 1%  (w/v) agarose gel and bioanalyser. Small RNA libraries were generated from both samples by using the Illumina Truseq Small RNA Preparation kit at LC Sciences. The purified cDNA libraries were sequenced on Illumina GAIIx, and raw sequence reads (36 nt) were obtained by using Illumina’s Sequencing Control Studio software version 2.8 followed by real-time sequencing image analysis and base-calling by Illumina’s Real-Time Analysis version 1.8.70 (LC Sciences).