GSM3964289: WT_2: Wildtype 2; Mus musculus; RNA-Seq
Total RNA was isolated from i) wild-type and miR-26-TKO (mR-26a-1-/-; miR-26a-2-/-; miR-26b-/-) primary SVFs or ii) miR-26a transgenic primary SVFs with and without doxycycline treatment using the miRNeasy Mini Kit (Qiagen) including an on-column DNAse I digestion step. RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) and sequenced using the 75 bp single-read protocol on a NextSeq 500 platform (Illumina).