Illumina HiSeq 1500 sequencing; Retrograde signalling during caused by heritable mitochondrial dysfunction is partly mediated by ANAC017 and improves plant performance.
Retrograde signalling during caused by heritable mitochondrial dysfunction is partly mediated by ANAC017 and improves plant performance.
One petri-dish with approximately 20 seedlings were collected and snap frozen in liquid nitrogen MS media with 2% sucrose and stratified for 2-3 days at 4°C, then grown under long-day conditions (16 h light/8 h dark) at 22 °C and 100 μmol m-2 s-1 Sigma Spectrum Plant RNA kit, sigma on-column DNase treatment, Turbo DNA-free kit treatment Illumina RiboZero Plant RNA library kit