PacBio transcript analysis of Zea mays genotype NC338, plant P1 in chamber C6 under Amb ozone.
Approximately 175 mg of leaf tissue was submitted to the UF ICBR for RNA extraction, library synthesis and sequencing. Total RNA was isolated followed by full-length cDNA synthesis using the SMARTer PCR cDNA Synthesis kit. Two cDNA reactions were performed for each sample. Single molecule SMRTbell libraries were constructed from each double-stranded cDNA sample using the IsoSeq protocol followed by PCR optimization and ELF size selection.