GSM3405591: e9.5_placenta_rep3; Mus musculus; ATAC-seq
Placenta were microdissected from e9.5 timed-pregnant mice and collected in cold dPBS We performed tissue homogenization and cell lysis, following the transposition and purification steps as described previously by Buenrostro et al.( 2015). We amplified the purified DNA with adapters from the Nextera index kit (Illumina). PCR purification was performed using AMpure XP beads (Beckman Coulter) in order to remove large fragments and remaining primers. Library quality was assessed using the Bioanalyzer High Sensitivity DNA Analysis kit (Agilent) to check for proper periodicity, and the DNA concentrations were estimated using the Qubit and Bioanalyzer. Libraries were sequenced by Elim Biopharmaceuticals, Inc. using the Illumina HiSeq 2500 Rapid run platform with 50bp paired-end sequencing.