16S rRNA-seq of  bacteria: industrialized Zhacai paocai 80d
Samples in different fermentation time were collected. Microbial DNA was extracted using the DNA kit. The V3-V4 and ITS1 region of the bacteria and fungi were amplified by PCR. Purified amplicons were pooled in equimolar and paired-end sequenced on an Illumina MiSeq platform.