miRNA-Seq of mus musculus: 4T1-derived exosome
Total RNA from BMDMs and 4T1-derived exosomes was used for miRNA sequencing. Library preparation and miRNA sequencing were performed by Ribobio. Briefly, RNAs were ligated with 3RNA adapters and then with 5RNA adapters, and the adapter-ligated RNAs were subject to RT-PCR and amplified using a low-cycle protocol. The PCR products were purified on PAGE gels and sequenced using an Illumina HiSeq 2500 platform. Raw data of the miRNA sequencing were further processed and the miRNA expression level was evaluated as RPM (reads per million).