16S human gut microbiota: sample MI-390_D0_MB
Total microbial DNA extracted from human feces (PowerSoil DNA Isolation Kit, MoBio). V4 region of the 16S rRNA gene amplified using primers F515 and R806. Sequencing performed in the Illumina MiSeq V2 platform using the dual-index sequencing strategy (Kozich et al. 2013, Appl. Environ. Microbiol. 79(17):5112).