GSM3040283: Zfp281 KO1_RNA-seq; Mus musculus; RNA-Seq
Total RNA was extracted with RNeasy Mini kit (QIAGEN). For ChIP-seq, chromatin was sonicated with Covaris S2. 150 ng of total RNA from each sample was used for library preparation. mRNA was purified with NEBnext poly(A) mRNA magnetic isolation module (NEB), and sequence libraries were prepared with NEBnext Ultra II Directional RNA library prep kit (NEB) following the manufacturer's protocol with 10 cycles of PCR amplification. ChIPmentation was carried out for the ChIP-seq library construction with 7 min incubation with a Tn5 tagmentation enzyme solution (Schmidl et al., 2015).