WGS of agricultural bulk soil: conventional tillage sampled at 0-10 cm from the Juchowo trial, replicate 1
DNA was extracted according to the phenol-chloroform protocol described by Lders et al. (2004). DNA was sheared using an E220 Focused-ultrasonicator (Covaris, USA). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (both New England Biolabs, UK). Sequencing was performed on a MiSeq sequencer using a MiSeq Reagent Kit v3 for 600 cycle (Illumina, USA).