WGS of S. cerevisiae: delta nup1 mutant6
"Yeast gDNA libraries were prepared using the Nextera XT kit (Illumina, San Diego, CA, USA) according to manufacturer's instructions.  Libraries were constructed using 0.7 ng of genomic DNA as starting material. Briefly, fragmentation of gDNA (5µl) was carried out in 10 µl of Tagment DNA buffer using 5 µl of Amplicon Tagment Mix. Indexes were added to Tagmented DNA by the use of 12 PCR cycles. The amplified and indexed libraries were purified and size selected  using Agencourt Ampure XP beads (Beckman Courter). The length distribution of the libraries was monitored using DNA 1000 kits on the Agilent 2100 Bioanalyzer (Agilent). "