GSM2610637: Wild-type Villus RNA PolII ChIP-seq biological rep1; Mus musculus; ChIP-Seq
Whole cell extracts from villi fixed with 1% formaldehyde were sonicated and incubated overnight with 5 ug RNAPII antibody (Santa Cruz, sc-899) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.