Single cell targeted DNA seqeuencing of homo sapien: peripheral blood/whole bone marrow mononuclear cells: Sample MSK33
"Single cells were encapsulated using a Mission Bio Tapestri microfluidics cartridge, lysed, and barcoded. Barcoded samples were then subjected to targeted PCR amplification of a custom 109 amplicons covering 31 genes known to be involved in hematologic malignancies. PCR products were removed from droplets, purified, and used as a template for PCR to incorporate Illumina i5/i7 indices. PCR products were repurified, quantified and pooled to be sequenced. Library pools were sequenced on an Illumina NovaSeq."