GSM1167070: K562; Homo sapiens; RNA-Seq
Total RNA was isolated from K562, HL-60 and THP-1 cells using TRIZOL® Reagent. The approximately 18-30 nucleotide RNAs were excised from denaturing polyacrylamide gel, ligated to sequencing adaptors on both ends, and reverse transcribed using small RNA sample prep kit (Illumina). The resulting cDNA libraries were PCR-amplified for 15 cycles and purified on acrylamide gel.