Correction data.P39
Lungfish gDNA for genome correction was isolated by standard gDNA isolation protocol. brief, snap frozen muscle tissue (0.3 g) was powdered and transferred into SDS buffer with Proteinase K (10 _g/ml) and incubated overnight at 65_. DNA solution was purified by two sequential phenol/chloroform extractions. After DNA precipitation, DNA pellet was re613 suspended in 1 ml of Elution Buffer (QIAGEN). RNase A treatment was carried out for 1h at 37C. The DNA solution was purified by two sequential phenol/chloroform extractions and DNA precipitation. gDNA was re-suspended in 100_l of TE buffer. Library preparation was performed using the Westburg NGS DNA library kit. The final library was excised by the Pippin prep with 400bp DNA size.