GSM4647549: R586W clone 1 rep2; Mus musculus; RNA-Seq
Cells were resuspended in 1ml Trizol (Invitrogen, 15596018). Chloroform (Sigma Aldrich, C2432) was added for phase separation. RNA was purified using the Zymo RNA Clean and Concentrator Kit (Zymo, R1013) Libraries were commercially prepared by Novogene (Sacramento, CA) using in-house library prep protocols.