arabidopisis_Col-0_rep_2(aos)
For each biological replicate, midgut tissue from 12 individual larvae (reared on a given host genotype) was pooled. Total RNA was extracted and isolated using a Qiagen RNAeasy kit. RNA samples were assessed for quality on a Bioanalyzer. Libraries were prepared using the mRNA-Seq8 Sample Prep Kit (Illumina), according to the manufacturer’s instructions. Libraries were prepared using the mRNA-Seq8 Sample Prep Kit (Illumina), according to the manufacturer’s instructions. Briefly, polyA-containing mRNA was purified and fragmented to an average size of approximately 2-300 nucleotides. The resulting fragmented RNA was reverse transcribed with random primers, made double stranded, end repaired, and A-tailed allowing ligation of the Illumina adaptors. Library molecules were then enriched by limited rounds of amplification, purified and analyzed for quality and size on an Agilent Bioanalyzer. All libraries were sequenced with a 75 bp paired-end sequencing run performed on the Illumina Genome Analyzer II.