GSM3265400: Col-0_20ºC; Arabidopsis thaliana; RNA-Seq
Leaf tissue was colected, inmediately frozen on liquid nitrogen and stored at -80ºC. For total RNA extraction, frozen tissue was homogenized to a fine powder and total RNA was extracted using TRIzol® Reagent (Life Technologies), treated with DNaseI (Roche) and further cleaned with RNeasy Plant Mini Kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols.