GSM1164897: rnaseq ko2; Mus musculus; RNA-Seq
Total RNA from cells was extracted with the TRIzol reagent (Invitrogen) and the RNeasy mini kit (Qiagen). Poly-A selected RNA was isolated from total RNA with MicroPoly(A)Purist Kit (Ambion) according to the manufacturer’s instruction. RNA-seq libraries were prepared following the dUTP protocol.