Pdan_filter_sense_MID7
The DNA library was quantified using a PicoGreen DNA Quantitation Kit (Life Technologies) and checked for quality by capillary electrophoresis (Agilent Bioanalyzer 2100 with the High Sensitivity DNA assay kit). The DNA library was then amplified in emulsion following the Titanium LIB-L emPCR protocol (Roche). The reaction was recovered by isopropanol emulsion breaking, and the beads carrying clonally amplified DNA fragments on their surface were enriched. The enriched sample was loaded onto one PicoTiterPlate (PTP) and was sequenced according to the 454 GS-FLX Titanium protocol on the GS-Junior sequencing platform.