GSM2701872: Corticospinal Neurons (Ctip2+) B6SJL-Tg(hSOD1*WT)2Gur/J replicate 1; Mus musculus; RNA-Seq
RNA was recovered from the FACS-purified cells as previously described (Molyneaux et al., 2015), quantified by spectral absorbance on the NanoDrop, and qualitatively assessed on the Agilent Bioanalyzer RNA-6000 Pico chip. RNA integrity numbers (RINs) for all samples ranged between 8.3 and 10. For each enriched sample, 10 ng of total RNA was reverse transcribed to synthesize cDNA using the Smart seq2 V4 kit (Clontech). Amplified cDNA was used as input for high-throughput RNA sequencing library preparation using the Nextera XT library kit (Illumina). Individual libraries were indexed, pooled, and sequenced to a mean depth of 5.466 x 107-125 bp paired-end reads per sample.