GSM3967004: 0h.R1; Mus musculus; RIP-Seq
Cells were cross-linked at 254 nm once for 400 mJ/cm2 and then again for 200 mJ/cm2 in the UV cross-linker. Then harvested and lysed cells in NP-40 lysis buffer The RNA libraries were prepared mainly as previous protocol [B. Zarnegar et al., Nat Methods 13, 489-492 (2016).] except with the use of SuperScript™ III Reverse Transcriptase for cDNA synthesis.