Illumina HiScanSQ paired end sequencing; Translational and transcriptional RNA-seq of SBDS cell lines.
Translational and transcriptional RNA-seq of SBDS cell lines.
Fractions were collected from sucrose gradient after ultracentrifugation. RNA was extracted by phenol/chloroform/isoamyilic acid method. Libraries for Illumina sequencing were constructed from 100 ng of total RNA with the Illumina TruSeq RNA Sample Preparation Kit.