Seqeunce Data of Agricultural Soil
Amplicons were generated with the primer set F515/R926 (Quince et al. 2011), which consisted of barcoded forward primer was ligated to PGM linker primer/ adapter, a Golay barcode(Hamady et al. 2008; Whiteley et al. 2012). The amplification condition was 95ĘĄC for 3Ęminutes initial heating followed by 30Ęcycles of 1Ęmin denaturation at 95 ĄC, 45Ęsecs alignment at 55ĘĄC, 1 minute extension at 72ĘĄC and a final extension at 72ĄC for 10 mins. Amplicon samples purified using Agencourt Ampure XP purification Kit (Beckman Coulter Ltd, UK) and quantified using Qubit¨ dsDNA HS Assay Kit (life technologies) were pooled to create an equimolar library of 500 pM DNA.