GSM2610640: Wild-type Villus CTCF ChIP-seq biological rep1; Mus musculus; ChIP-Seq
Whole cell extracts from villi fixed with 1% formaldehyde were sonicated and incubated overnight with 7 ul CTCF antibody (EMD Millipore, 07-729) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.