Amplified SSU rRNA iTag sequences arctic thermokarst lake sediments
Total RNA was extracted from ~ 2 g of sediment (in duplicate) using a modified protocol of MoBio's PowerSoil Total RNA Isolation Kit, and pooled prior to RNA amplification with the MessageAmp II aRNA Amplification Kit (Life Technologies). Amplified RNA was reverse transcribed, cDNA was amplified with primers targeting the SSU rRNA gene V4 region, and then sequenced.