Bistable Expression of CsgD in Salmonella Connects Virulence to Persistence
RNA was treated with the MICROBExpress™ Bacterial mRNA Enrichment Kit (Life Technologies #AM1905) prior to preparing cDNA. Libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Final library fragment sizes were 200-300 bp.  This approach incorporates dUTP into the second cDNA strand synthesis followed by digestion of the second strand before PCR amplification.