RNA-Seq of Apis mellifera pupa 3 days post-injection with WT virus USA 2015
A pink-eye honeybee pupa from a Florida brood frame with low Varroa infestation and which had no observable Varroa mite feeding exposure, was intra-abdominally injected into the hemolymph to introduce 5 microgram of wild-type RNA extracted from a DWV virus particle preparation (see BioSample SAMN08430537). After a 3 day-long incubation (3 dpi) to allow replication of DWV, total RNA was extracted for Illumina TruSeq library preparation and sequencing.