UCE target enrichment of luscinia-svecica_UAM13718
Double-indexed DNA libraries were then prepared for each sample as described in Glenn et al. (2016), which were then each quantified with a Qubit fluorimeter (Invitrogen, Inc.). The libraries were then combined into equimolar pools. The pools were enriched using the Tetrapods-UCE-5Kv1 kit (MYcroarray) for a set of 5,060 loci, using UCE enrichment protocol version 1.5 and post-enrichment amplification protocol version 2.4 (ultraconserved.org) with HiFi HotStart polymerase (Kapa Biosystems) and 14 cycles of post-enrichment PCR. Distribution of fragment size of the enriched pool was quantified on a BioAnalyzer (Agilent, Inc), and the enriched pool was quantified by qPCR with a commercial kit (Kapa Biosystems). We sequenced all pools using an Illumina HiSeq 2500.