light chain antibody sequences from 8 weeks post-HIV-infection
gene specific primers targeting IGLV3-21 and constant region primers specific Lambda (targeting the CH1) were used to PCR amplify relevant antibody sequences. All PCRs were don't 7 times and pooled per time point. Each time-point was then indexed using Illumina Nextera indexes and all time-points pooled and loaded onto an Illumina Miseq