GSM1448290: Nanog-GFP ESCs; Mus musculus; Bisulfite-Seq
Bisulphite treatment and sequencing library preparation were performed as previously described (Lister et al., 2011). Briefly, genomic DNA from each iPSC and ESC was fragmented by sonication prior to ligation of methylated sequencing adaptors. Fragments were size-selected by gel electrophoresis and purification, treated with sodium-bisulphite and amplified with 4 cycles of PCR to generate libraries that were sequenced on the Illumina HiSeq platform.