GSM1646303: Glucose time course, 5 hour time point, biological replicate 3; Escherichia coli; RNA-Seq
RNAsnap followed by RiboZero rRNA removal kit for gram-negative bacteria (Epicentre) RNA was fragmented using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Then, RNA was phosphorylated using T4 polynucleotide kinase and prepared using the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible). DNA fragments greater than 100 bp were excised from a 4% agarose gel after library preparation and recovered using the Zymoclean Gel DNA Recovery Kit (Zymo Research).