RNA-Seq of Homalodisca vitripennis: 5th instar mesonotum
Mesonota were dissected from 5th instar individuals preserved in RNALater. Tissues were pooled between 4 individuals for pool A. RNA was extracted and polyA enriched with Sera-Mag Oligo (d)T beads. Sequencing libraries were prepared with the KAPA Stranded RNA-Seq kit, using New England Biolabs adapter and primer. This is a stranded library using the (d)UTP method.