GSM4697819: PFDN2-Post-stimulation-rep1; Homo sapiens; RNA-Seq
Total RNA was extracted with RNAeasy mini kit (Qiagen), and treated with DNase previous to retrotranscription. 1 µg of DNase treated RNA was retrotranscribed by Super-Script III (Invitrogen) using random hexanucleotides as primers. Total RNA was depleted of 18S rRNA and then libraries were prepared.