mRNA sequencing of Arctic charr gill tissue (Family 12; seawater; low tolerance; no. 784)
Poly-A mRNA was isolated from total RNA and purified using ploy-T oligo-attached magnetic beads. mRNA was fragmented into pieces using divalent cations under elevated temperature, and copied into cDNA using reverse transcriptase and random primers. Following the ligation of adaptors, cDNA templates were purified and amplified with PCR.