ChIP-Seq for human NK cell, CD56dim CD16+ - 27_CHIP_H3K27AC_NK_CD16POS
Cell types from 6 donors were utilized for this analysis. A total of 30 ChIP-Seq assays were performed as described previously (Youhanna Jankeel et al., 2018). Briefly, sheared chromatin from each cell type was immunoprecipitated with a polyclonal anti-H3K27ac antibody (C15410196; Diagenode) by use of an automated ancillary liquid handler SX-8G IP-Star from Diagenode. Immunoprecipitated chromatin was captured, washed, Illumina library adaptors integrated by transposase-based method (Youhanna Jankeel et al., 2018) and library prepared by PCR amplification. Libraries were sequenced on an Illumina HiSeq 2500 sequencer to obtain 50-bp single-end reads.