GSM2602049: IRI48h-R2; Mus musculus; RNA-Seq
Qiagen Rneasy mini kit Illumina TruSeq RNA Sample Prep kit v2 protocol was followed with the exception that the final PCR amplification was performed for 12 and not 15 cycles. Libraries were visualized on the Agilent Bioanalzyer and quantified using the Kapa Biosystems Library Quantification Kit according to manufacturer’s instructions.  Libraries were applied to an Illumina flow cell at a concentration of 16 pM on a version 3 flow cell and run on the Illumina HiSeq 2000 as a paired end read for 100 cycles each side.  On average, 3 samples were loaded to each lane of a flow cell.