Illumina HiSeq 2500 paired end sequencing; RNA sequencing of purified intestinal epithelial cells from paediatric biopsies including Inflammatory Bowel Disease and healthy controls
RNA sequencing of purified intestinal epithelial cells from paediatric biopsies including Inflammatory Bowel Disease and healthy controls
Gastrointestinal biopsies were taken from three gut segments during diagnostic endoscopy. Ascending colon (AC), Sigmoid colon (SC) and terminal ileum (TI). Intestinal epithelial cells were extracted using magnetic bead sorting for EPCAM positive cells. AllPrep DNA/RNA mini kit (Qiagen, Germany) following the manufacturer's instructions Agilent Bioanalyzer was used to check RNA integrity following the manufacturer's guidelines. mRNA was sequenced at the University of Kiel, Germany. TruSeq stranded Total RNA kit (Illumina Inc) was used to construct strand-specific sequencing libraries from total RNA. TruSeq stranded Total RNA kit was used to construct strand-specific sequencing libraries from total RNA.