Illumina Genome Analyzer II sequencing
small RNA
miRNAseq libraries were prepared starting from purified small RNAs isolated with mirVana miRNA Isolation kit (Ambion) according to manufacturer’s instructions. miRNAs were isolated from C2C12 mouse myoblasts and myotubes. Myoblasts C2C12 were grown on collagen-coated plates in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% glucose and 2% glutamax (Invitrogen). Differentiation was induced by serum deprivation for 7 days, by culturing in DMEM supplemented with 2% FBS, 1% glucose and 2% glutamax. Cells were grown under 10% CO2. Sequencing libraries were prepared according to the method described by Buermans et al. (BMC Genomics 2010, 11:716, doi:10.1186/1471-2164-11-716).