DNA-seq of Amazonian environmental sample
40 pooled samples (all litter depth) from 28 m radius circular plot; 15 ml DNA extracted using MoBio Soil Maxi kit; PCR targeting COI region of ~313 bases using an internal forward primer (5' - GGWACWGGWTGAACWGTWTAYCCYCC - 3') and the COI degenerate reverse primer (5' – TAAACTTCAGGGTGACCAAARAAYCA - 3') of metazoa; Macrogen Companie amplification and Illumina MiSeq sequencing