Whole genome resequencing of Nasonia vitripennis, adult female
For DNA extraction, each individual was put in a 1.5 ml tube and rinsed once with 1 ml 70% ETOH, 1 ml 10% bleach, and twice with 1 ml sterile water. Samples were then frozen in liquid nitrogen with additional mechanical homogenization. DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. Samples were quantified using the Qubit dsDNA HS Assay kit on the Qubit 2.0 Fluorometer (Life Technologies).