The effect of 2-phenylethanol treatment on Aspergillus flavus transcriptome- Treated       48hr
Aliquots of the Aspergillus flavus NRRL3357 spore suspension were         inoculated into each 20 ml of NYDB medium in a 125 ml flask to a final concentration of         100,000 /ml. For the treatment set, 2-phenylethanol was added to the culture at a final         concentration of 1ul/ml. Cultures in triplicates for the control and treatment sets were         grown at 28°C on a rotary shaker at 150 rpm. At 24h, 48h and 72h after inoculation, mycelia         were harvested, rinsed with cold DEPC-treated water and total RNAs were prepared from ground         mycelia.