GSM2702322: Huh6 rep4; Homo sapiens; RNA-Seq
At indicated days tumors or non-implanted CAMs were isolated, washed in PBS and snap frozen in liquid nitrogen. RNA extraction was carried out using the mirVanaTM Isolation Kit (Ambion) according to the manufacturer's protocol (see Fig. 1). RNAs of Huh6 cells in culture were also extracted with the same kit according to the manufacturer's protocol. RNA quality was verified using Agilent RNA 6000 Nano kit on an Agilent 2100 Bioanalyzer (Agilent Technologies). Four μg of RNA were used to generate sequencing library using the TruSeq Stranded mRNA Sample Preparation kit (Illumina) according to the standard protocol. Library preparation and sequencing were performed by the MGX-Montpellier GenomiX platform.