GSM2525709: MeDIP-seq KO rep2; Mus musculus; MeDIP-Seq
Genomic DNA from freshly isolated islets were extracted using DNeasy Blood Tissue Kit (Qiagen, GmBH, Germany). MeDIP was performed using a MagMedIP kit (Diagenode, Denville, NJ, USA) as previously described69. Libraries were prepared following the manufacture’s instruction and were sequenced by Illumina HiSeq 2500 with 50 cycles.