GSM1647126: Control Experiments Set 3; Homo sapiens; OTHER
Intact nuclei were isolated with ATAC-seq cold lysis buffer suplemented with protease inhibitors. Genomic DNA in intact nuclei was simultaneously molecularly tagged and fragmented. Nuclei tagged with 1 of 96 barcodes were pooled and then redistributed into wells of a PCR plate (15-25 nuclei per well) using a cell sorter.  A second barcode was introduced during library amplification.