GSM2704059: DOCK8 KO 1 not activated; Homo sapiens; RNA-Seq
KHYG1 cells were cultured as indicated prior to stimulation with anti-Nkp30 (duplicate samples). After 90 minutes, cell pellets were collected and total RNA was extracted using the Nucleospin® RNA extraction kit (Macherey-Nagel). RNA quality was checked on the Agilent 4200 Tapestation and RNA with RIN values > 9 were used for the subsequent analysis. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) according to the manufacturers instructions using 200ng total RNA input. Single-end 75bp RNA-sequencing was performed on the NextSeq 500 (Illumina).