GSM2790563: iPSEN1*-cPC-2; Homo sapiens; RNA-Seq
Cells were trypsinized, spun down, washed with PBS, and the cell pellet was resuspended in Trizol (ThermoFisher). RNA was isolated using chloroform, washed with isopropanol and 70% ethanol, and resuspended in RNAase-free water KAPA Biosystems KAPA stranded mRNA-seq kit with KAPA mRNA capture beads