GSM2254668: Host Stem, replicate 1; Arabidopsis thaliana; miRNA-Seq
Small RNA was extracted from ~ 100 mg of host stem, interface, and parasite stem tissue using the mirPremier microRNA Isolation Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol. Plant tissues were ground to a fine power in liquid nitrogen using mortar and pestle. Small RNA was analyzed using an Agilent small RNA Kit on a 2100 Bioanalyzer platform. Illumia Tru-Seq Indexed small RNA kit