GSM1645704: OCI-Ly7 rep2; Homo sapiens; ChIP-Seq
Chromatin was sheared to 200-400bp, incubated with H3K4me2 antibody (abcam 32356) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr.  Final chormatin was purified using Qiagen PCR purification columns. Illimunia Truseq ChIPseq protocol