GSM2522388: X061422; Homo sapiens; RNA-Seq
The total RNA was extracted using TRIZOL Reagent (Cat#15596-018, Life technologies, Carlsbad, CA,US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent Technologies), RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea,   CA,USA) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany) were used to purify total RNA RNA-seq libraries were prepared using TruSeq RNA Sample Pre Kit v2 –Set A/ B (Illumina).