GSM2791380: Donor14 Vehicle; Homo sapiens; RNA-Seq
Treated samples were lysed in RLT buffer and total RNA was extracted using the RNeasy Kit (Qiagen) according to the manufacturer's instructions. Integrity was assessed on the Agilent Tape Station 2200 requiring a RIN score greater than 8. 200ng total RNA was used as the starting material. PolyA selection was performed using the NEBNext PolyA selection Kit (NEB) followed by stranded library production using the NEBNext Ultra Directional Library Kit. Samples were sequenced on a NextSeq 500 42bp paired-end.