GSM2298157: PJ01; Pseudomonas aeruginosa; RNA-Seq
When reached OD600=1, cells were harvested in RNA protect bacteria reagent  (Qiagen) and disrupted with 2.8 mm  and 0.25 mm ceramic beads in a TissueLyzer II (Qiagen) acording to manufacturer's recommendations. Total RNA was extracted using the RneasyPlus 96 kit (Qiagen), Depletion of rRNA from total RNA samples was performed with the Ribo-zero rRNA Removal reagents (Epicentre). Libraries were constructed using the TruSeq Stranded mRNA HT Sample Prep kit (Illumina). The libraries were quantified with the Picogreen fluorescent dye and yielded around 200 to 800 ng.