NextSeq 500 paired end sequencing; Comparison of seven single cell Whole Genome Amplification commercial kits using targeted sequencing
Comparison of seven single cell Whole Genome Amplification commercial kits using targeted sequencing
H1 human ES cells (WA01) were obtained from the WiCell Research Institute (Madison, WI). In order to create single cell (SC) clones, SCs were picked and deposited in separated single 96 wells using an automated cell picking device (CellCelector, ALS). Cells were cultured and treated as described in (Biezuner et al. Genome Research. 2016) DNeasy Blood & Tissue Kit, Qiagen Targeted enrichment using Access Array (Fluidigm) using the protocol as described in (Biezuner et al. Genome Research. 2016.) Sequencing primers are described in manuscript Supplementary Table 1.