RNA-Seq of Plasmodium falciparum Dd2 : R treatment Replicate 1
Quantity and quality of the total RNAs (3 biological replicates) extracted from the schizont parasites by  TRIZOL standard  procedure, and  determined via a Bioanalyzer 2100 (Agilent) and a Qubit 2.0 Fluorometer (Life Technologies). Four g of total RNA was used to prepare the cDNA library according to the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems) production manual, with one adjustment of 62C as the elongation temperature in PCR. The cDNA was proceeded for the paired-end (2150 nt) genome-wide sequencing via an HiSeq X Ten sequencer (Illumina). Paired-End 159-nt clean reads from each FASTQ file were mapped separately to P. falciparum reference genomes