Whole genome sequencing of Colocasia esculenta: leaf tissue of vegetative 8-month-old Niue plants, 10-15kb mate pair library 1
The mate-pair library was constructed with the Nextera Mate Pair library Sample Prep kit (Illumina, CA), followed by the TruSeq DNA Sample Prep kit.  The mate-pair library (with an insert size of 10-15kb) was quantitated by qPCR and sequenced for 161 cycles from each end of the fragments on a HiSeq2500 using a Rapid TruSeq SBS kit version 2. Fastq files were generated and demultiplexed with with the bcl2fastq v1.8.4 Conversion Software (Illumina).