RNA-seq of mouse wt and SDF-1/CXCL12 ko heart tissue
Hearts were removed, flash frozen on dry ice, and total RNA was extracted using Trizol reagent. RNA integrity was verified with the Agilent Bioanalyzer. Ion Torrent compatible libraries were generated from 20ng total RNA input, using the QuantSeq 3 mRNA-Seq Library Prep Kit from Lexogen. RNA-Seq was conducted on an Ion Proton instrument (ThermoFisher) to obtain ~20 Mio Reads per sample.