GSM2789861: DLD1-siY14-9-exp3-rep2; Homo sapiens; RNA-Seq
Total RNA extraction was performed using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions after homogenisation of each samples with a QIAshredder column (Qiagen). A DNase step was additionally performed during RNA isolation as described by the manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols.The standard protocol consists of transcripts purification by polyA-tail selection, followed by library construction using illumina TruSeq Stranded mRNA Library Prep kit.