Estrogen alters the profile of transcriptome in river snail Bellamya aeruginosa
The total of RNA was extracted from hepatic and testis tissues in river snails exposed to 17β-estradiol or the blank samples, and then the mRNA was isolated, purified and treated to short fragments. After that, the double-stranded cDNA was synthesized and separate adaptors were ligated to cDNA fragments from each cDNA library. And Fragments were then sequenced with Roche/454 GSFLX Titanium instrument.