GSM1647362: Dm_Rpb3_X_ChIP_(20140917_3); Drosophila melanogaster; ChIP-Seq
Drosophila S2 cells were crosslinked using formaldehyde for 10 minutes at room temperature. Chromatin was fragmented using MNase and then sonicated to ensure complete extraction of chromatin and associated proteins. Chromatin was immunoprecipitated using an antibody against Rpb3. DNA was extracted from the immunoprecipitated chromatin and subjected to Illumina (Solexa) sequencing.