GSM2298383: SJSA, polysomal RNA, DMSO treated, replicate 2; Homo sapiens; RNA-Seq
Polysomal fractions were separated on linear sucrose gradient by ultracentrifugation. RNA was purified using phenol/chloroform extraction. RNA quality was assessed using Bioanalyzer RNA Pico chips (Agilent). PolyA RNA was purified from 15 g of total RNA using the Dynabeads mRNA Direct micro kit (Life Technologies) according to the manufacturer’s instructions. Libraries for Ion Torrent sequencing were prepared using the Ion Total RNAseq v2 kit (Life Technologies), according to the manufacturer’s instructions.