GSM2254828: A4; Nitrobacter winogradskyi; RNA-Seq
Cells were collected by centrifuging, lysed by sonication, and total RNA was extracted with a Qiagen Rneasy Kit. Ribosomal RNA was depleted with a MICROBExpress Bacterial mRNA enrichment kit. An Illumina-targeted RNA Expression Kit and at least 200 ng of RNA containing less than 5% rRNA were used to prepare mRNA-Seq libraries. The libraries were sequenced (150mer paired-end) on a HISEq 3000 Sequencer (Illumina, San Diego, CA) at the Center for Genome Research and Biocomputing Core Laboratories at Oregon State University. mRNA-Seq.