GSM2254820: SLE Sample 4 methyl-seq; Homo sapiens; Bisulfite-Seq
Purified genomic DNA was isolated from frozen aliquots containing PBMCs using standard phenol/chloroform extraction protocols. Sample gDNA was fragmented on a Covaris S2 sonicator to an average size of ~350bp in length.  Fragmented DNA was subjected to MethylMiner Methylated DNA Enrichment Kit. Illumina sequencing libraries were prepared using the Illumina Truseq DNA LT Sample Prep Kit.