RNA-Seq of Artibeus jamaicensis: adult liver
Total RNA was extracted from hepatic tissue with the RNAeasy extraction kit from QIAGEN19 with a DNAse clean step following the manufacturers instructions. RNA quality was measure with Nanodrop, Qubit 2.0 fluorometer and Bioanalyzer 2100 instruments. Library was constructed with 1.0 l of total RNA using the KAPA Stranded RNA-Seq kit with RiboErase from KapaBiosystems. Ribosomal RNA was removed by depletion with DNA primers hybridization followed by a treatment with RNAsa and DNAsa, according to the manufacturer protocol. Fragmentation cycle was adjusted to 10 cycles of amplification at 94C for 5 minutes to obtain fragments between 100 and 2100bp. Each library was enriched with Illumina TruSeq Index Adapters (250nM) for multiplex sequencing. Library quality was evaluated using Qubit 2.0 fluorometer and the Agilent Bioanalyzer 2100; good quality libraries were paired-end (PE) sequenced on the Illumina HiSeq 4000 at the Center of Genomics Services from the University of California, Berkeley.