Identification of miRNAs in leaves of the sample "xanthuim-hubei"
Small RNAs with a length of 18-30nt were purified from total RNA of the sample "xanthium-hubei".After being ligated to a 5' and 3' adaptor and RT-PCR,the amplified products were used for sequencing.Small RNA reads with a length of 49nt were produced by Illumina and then were used to do data processing, miRNA identification, expression analysis and target prediction.