GSM2610665: Cdx2-/- Villus H3K27me3 ChIP-seq 5+7d biological rep1; Mus musculus; ChIP-Seq
Whole cell extracts from villi fixed with 1% formaldehyde were sonicated and incubated overnight with 5 ug H3K27me3 antibody (EMD Millipore, 07-449) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.