Mycobacterium lepraemurium genome sequencing
DNA was extracted from isolated bacteria grown in Balb/c mice. Fifty ?l of DNA was sonicated with the S220 Covaris to obtain 400 bp-long DNA fragments and purified using AMPure beads (1.8X). After quantification with the Qubit dsDNA HS assay and the Qubit Fluorometer, up to 1 ?g of DNA in 50 ?l was used for library preparation using the Kapa Hyper prep kit (Roche) and the PentAdapters (Pentabase) for indexing. The library was quantified using the Qubit dsDNA BR assay and the Qubit Fluorometer.