RNA-Seq of seawater  mesocosm - control day 4
Total RNA (8 g) was sent to the sequencing facility at the University of Cambridge (Cambridge, UK) where it was subjected to Ribo-Zero ribosomal RNA removal prior to library preparation using Illumina TruSeq Stranded mRNA kit (Illumina). Paired-end (75 bp) sequencing was carried out on a NextSeq500 platform using the 150-cycle kit.