E.coli ORFeome capture
DNA target sequences (full lenght ORFs) were captured from total genomic DNA of E.coli K12 using LASSO probes  and simultaneously amplified by PCR using common primers located on the Long Adapter sequence of the LASSO probes . PCR amplicons containing ORFs were subsequently shared by sonication, subjected to end repair, 5' phosphorylation, dA-tailing , Illumina adaptor ligation and sequenced.