Monanchora arbuscula 16S amplicon
Genomic DNA was PCR amplified with primers CS1_515F and CS2_806R, modified from the primer set employed by the Earth Microbiome Project (GTGYCAGCMGCCGCGGTAA and GGACTACNVGGGTWTCTAAT) targeting the V4 regions of microbial small subunit ribosomal RNA genes. Amplicons were generated using a two-stage targeted amplicon sequencing protocol as described previously (Naqib et al., 2018).