GSM1646760: Seedling_degradome2_raw; Phaseolus vulgaris; OTHER
Total RNA was isolated from frozen samples using the Trizol reagent according to manufacturer's instructions (Invitrogen, Carlsbad, CA) and 40 µg of each sample were prepared The protocol used to obtain cDNA libraries for degradome analysis was carried out essentially as previously described (Addo-Quaye et al, 2008; German et al, 2009)