GSM4296991: sigG1 mutant - sample1; Streptomyces tsukubensis NRRL18488; RNA-Seq
Total RNA was isolated using the RNeasy® Mini kit (Quiagen) as in Beites et al. (2011) . TruSeq Stranded Total RNA with Ribozero Library Preparation Kit was used for the construction of sequencing cDNA libraries. The generated DNA fragments were sequenced in the Illumina Novaseq 4000 platform, using 150bp paired-end sequencing reads.