GSM3263287: IR12h_IF12_rep2; Mus musculus; RNA-Seq
Total RNA was extracted from the frozen brain tissue samples using a micro-tube tissue homogenizer and EZ-10 DNAaway RNA extraction mini-prep kit following manufacturer's instruction. Samples with only RIN above 7 were seleted for the rest of the procedures. Purified mRNA was used for library preparation for sequencing using standard Illumina protocols.