anoxygenic photosynthesis pufM gene amplicon library: freshwater bacterial community
Water was filtered through 0.22 m Sterivex-GP filters. DNA was extracted and the pufM gene (encoding for photosynthetic reaction center M subunit) was amplified. Amplification was done in triplicate, which were pooled, purified from gel and sent for sequencing on Illumina MiSeq in 2x250 bp reads.