GSM3405586: Sham JIB-04; Mus musculus; RNA-Seq
Total RNA was extracted with TRIzol reagent (Invitrogen) by following manufacturer's protocol, followed by clean-up and DNase I treatment with Ambion kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Library construction was performed using Illumina Truseq mRNA-LT (cat. RS-122-2101) kit by following manufactuer's protocol.