Illumina metagenome of Mushroom Spring: 60C, 1000-1500um
Mat samples were collected using a #4 cork borer on 2009-12-09, immediately frozen on dry ice in the field, and kept frozen at -80C until analysis. Frozen cores were embedded in Tissue-Tek O.C.T compound and sliced parallel to the mat surface at 40um intervals using a cyrotome (Leica CM 1850), with adjacent slices combined. DNA was extracted and purified with the fastDNA spin kit (Molecular Biosciences) according to the manufacturer's instructions. Cells were suspended in the manufacturer's lysis solution and lysed by bead beating in a Fastprep cell disrupter (Bio101 Savant Instruments) for 45s at a speed of 6.5 m/s.