GSM2790937: GM12878 SALP-seq; Homo sapiens; OTHER
The nuclei of 100,000 GM12878, HeLa, HepG2, and 293T cells, and various numbers of HepG2 cells (50,000, 10,000, 5,000, 2,500, and 500) were extracted. Nuclei then tagmented by Tn5 transposome with different barcoded Tn5 adaptors.The denatured tagmented gDNA was ligated with variant single strand adaptors. Then elongated, purified with Ampure XP beads and amplified with Illumina compatible primers. The genome DNA of HepG2 were digested with Hind III and sonicated, and genome DNA libraries were constructed with SALP protocol.