RNAseq of mus musculus:  bone marrow derived dendritic cells
For RNA sequencing, samples were first processed for library preparation using the Ovation Human FFPE RNA-Seq Multiplex System Kit (NuGEN, 0340-32, 0341-32) and depleted for ribosomal RNA. Library was sequenced on HiSeq 2500 RapidRun flow cells to achieve high quality reads. RNA sequencing reads were aligned to the mouse genome 10 (mm10, GRCm38.90) using the RNA aligner STAR77. Next, the aligned reads were filtered on quality metrics (qm), loaded in IGV browser78 for purpose of visual inspection of the alignment used for quantification. Quality metrics aligned reads were quantified using PartekGS to obtain raw read counts and RPKM with gene annotation. Differently expressed genes were identified using ANOVA test to generate corrected or non-corrected p values in PartekGS.