WXS of mouse: Lys(+) GPX(+/-) FvB AOM, male, 288951
Genomic DNA extractions from fresh frozen mouse tissues or cultured cells were performed using the DNeasy Blood and Tissue Kit (Qiagen). DNA Exome Capture library preparation was performed from 1 ľg of the extracted mouse tissue or cell culture genomic DNA using SureSelect XT Mouse All Exon (Agilent Technologies) with four cycles of pre-capture and 10 to 12 cycles of post-capture amplification. The libraries were diluted to 2 nM or 10 nM, according to their final concentration, and clustered at 10 pM using the TruSeq PE Cluster Kit v3 - cBot - HS or the HiSeq Rapid PE Cluster Kit v2. Exome libraries were sequenced either separately in one lane of a high output flowcell or as a pool of four libraries spread over two rapid flowcells v2 (4 lanes).