RNAseq of Copaifera langsdorffii: CER
RNA extraction was performed individually from samples collected from Atlantic Rain Forest and Cerrado. The total RNA was extracted following the modified Japelaghi protocol, 10 g of each RNA samples were sent to Fasteris Life Sciences SA (Plan-les-Ouates, Switzerland), where the quality and quantity was ascertained by Qubit and Bioanalyzer Nano Chip. Ten individual leaf samples from each ecosystem were selected according to their quality, after passing through poly-A selection protocol. The treated samples were polled together in equal concentration amounts for sequencing. Multiplexed sequencing performed on Illumina HiSeq 2500 using the single-end 76 cycle protocol.