Water buffalo raw milk from manufacture 2
The microbial diversity was studied by pyrosequencing of the amplified V1-V3 region of the 16S rRNA gene by using primers Gray28F 5’-TTTGATCNTGGCTCAG and Gray519r 5’- GTNTTACNGCGGCKGCTG amplifying a fragment of 520 bp. 454-adaptors were included in the forward primer followed by a 10 bp sample-specific Multiplex Identifier (MID). After PCR, the amplicons were used as a template for pyrosequencing on a GS Junior platform, according to the manufacturer’s instructions by using a Titanium chemistry.