Illumina HiSeq 2500 paired end sequencing; Assessing genome instability in Bloom syndrome
Assessing genome instability in Bloom syndrome
Fibroblasts were cultured in DMEM (Life Technologies) supplemented with 10% v/v FBS (Sigma Aldrich) and 1% v/v penicillin-streptomycin (Life Technologies), B-lymphocytes in RPMI1640 (Life Technologies) supplemented with 15% v/v FBS and 1% v/v penicillin-streptomycin.  Protocols for single-cell sorting, Strand-seq library preparation (Sanders et al., 2017), and single-cell WGS library preparation (van den Bos et al., 2016) were previously described. Protocols for single-cell sorting, Strand-seq library preparation (Sanders et al., 2017), and single-cell WGS library preparation (van den Bos et al., 2016) were previously described.