GSM1647105: M372-Yers-4; Homo sapiens; RNA-Seq
We extracted RNA using the QIAgen miRNeasy kit. There were a total of 13 batches of 12 samples each (6 individuals x 9 conditions x 3 timepoints, minus 48 hours post-infection with Staphylococcus epidermidis). We designed the batches to maximally partition the variables of interest (individual, condition, timepoint) in order to minimize the introduction of biases due to batch processing. In batches of 12 samples (the same 13 from the extraction protocol), we added barcoded adapters (Illumina TruSeq RNA Sample Preparation Kit v2)