0 hour North Shore Channel river water + UV treated WWTP Effluent
River water collected from North Shore Channel at Touhy Ave. near the surface (0-1m depth), 50 ml combined with 50 ml treated effluent, and immediately passed in succession through ~1.6 m pore size glass fiber filter for pre-filtration and captured on a 0.22 m pore size polycarbonate membrane filter for collecting free-living prokaryotic component. RNA was extracted using phenol:chloroform based extraction, and used for RT-PCR amplification of 16S rRNA gene with primers 27F/534R. PCR products were submitting for sequencing in paired-end format. This library represents one of the duplicates for this time-point.