GSM4647555: SETX-KO_MeDIP-Seq; Homo sapiens; MeDIP-Seq
Genomic DNA was extracted and sonicated to ~150-300 bp using a Misonix Sonicator 300 equipped with a microtop. Illumina adaptors were added to the sonicated DNA and immunoprecipitated using a mouse monoclonal antibody against 5-methylcytosine (5meC; Clone 33D3, Active Motif) following the protocol described in the Active Motif MeDIP kit (Active Motif, Catalog No. 55009). Libraries were prepared according to Illumina's instructions. Deep sequencing libraries were generated from the DNA immunoprecipitates and input DNA by the standard consecutive enzymatic steps of end-polishing, dA-addition and adaptor ligation. After a final PCR amplification step using barcoded primers, the resulting DNA libraries were quantified and sequenced.