GSM3774199: sNPFR_p_16; Drosophila melanogaster; RNA-Seq
Small pools (15-60 neurons) and single GFP-labeled neurons were manually sorted by mouth aspiration using a glass micropipette from protease digested, acutely dissociated (TRH-Gal4) or pinned (sNPFR-Gal4) fly brains RNA was harvested from collected cells using the Picopure RNA Isolation kit, and cDNA libraries were generated from purified RNA using the SMART-Seq v4 Ultra Low Input kit (Clontech). 12 or 17 cycles of PCR amplification were used for cell pools or single cells, respectively. Next generation sequencing libraries were then prepared using the Nextera XT kit (Illumina)