High-throughput development of mapped SSR markers from pea (Pisum sativum L.) based on next generation sequencing of a purified Chinese commercial variety
Genomic DNA was extracted from 7-day old, etiolated seedlings of each genotype, using the CTAB method [17,18]. For Illumina HiSeq 2500 run, library was prepared with a commercial kit (NEBNext Multiplex Oligos for Illumina (Index Primers Set 2); New England Biolabs Inc., Ipswich, MA, USA) following the manufacturer’s protocol (Paired-End Library Construction).