Targeted sequencing of organellar genes of Flatbergium sp. BlS-2016: B. Shaw 17137
To reconstruct the backbone phylogeny of mosses, we sampled 134 species from 124 genera, 64 families and all but one of the 30 orders spanning the moss tree of life (Goffinet et al. 2009). We targeted 272 protein coding genes (122 organellar and 150 nuclear) based on the liquid phase bait hybrid method. Genomic DNA was extracted from approximately 0.4 to 4.0 g dried or fresh gametophytic or sporophytic moss tissue using the NucleoSpin Plant midi DNA extraction kit, and then sonicated to a fragment size of ~550 bp. NGS libraries were prepared using the Illumina TruSeq Nano DNA Library Preparation kit. Up to 96 indexed libraries were pooled, and enriched for the targeted genes using the custom designed MYbaits kit. The enriched, pooled libraries were paired-end sequenced on an Illumina MiSeq platform using the 600-cycle v3 sequencing kit. Organellar and nuclear genes were enriched and sequenced separately.