male-nose-freshwater
stranded libraries produced from 2μg of total RNA using TruSeq stranded total RNA sample Kit (Illumina, USA) according to the manufacturer’s instructions (Illumina #15031048 Rev.E). Sequencing was performed on a MiSeq instrument using a v3. MiSeq Reagent Kit (Illumina) generating 2x300 bp of strand-specific, paired-end reads. TruSeq Adaptor: A025(ACTGAT)