Illumina MiSeq paired end sequencing
333 stool samples from 54 infants sequenced to characterize bacterial microbiome in high and low risk for asthma infants in the first year of life.
DNA was extracted using lysing matrix E tubes and AllPrep kit (Qiagen) protocol; V4 region of 16S rRNA amplified with 515F and 806R; sequenced on Illumina MiSeq