GSM1646749: sac3∆ pRS315 vector_01; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted by hot Phenol method. DNA was digested, RNA purified by a second Phenol Chlorofom extraction. rRNA was removed with Ribozero Kit (Epicentre) following manufacturers instructions. Libraray was generated following the instructions of the NEB RNA ultra Kit for library preparation