GSM2703246: 5hmC-BC6T; Homo sapiens; OTHER
Genomic DNA from frozen samples was extracted from twenty 10-µm sections using the Qiagen-DNeasy Blood &Tissue Kit according to the supplier's instructions (Qiagen, Hilden, Germany). This included a proteinase K digestion at 55C overnight. DNA was quantitated with the NanoDrop® ND-1000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). Library preparation was done with the TruSeq ChIP Sample Prep Kit (Illumina) hMeDIP-Seq