GSM1464464: Cerebellum_BS-seq; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated from each brain region for wild-type, dnmt3a flx/flx, or dnmt3a flx/flx Nestin-cre mice at the indicated ages. For bisulfite treatment, 25-100 ng of genomic DNA 0.25-1 ng of unmethylated lambda DNA was added and libraries were generated using the Ovation Ultralow Methyl-Seq Library System (Nugen). Bisulfite treatment was performed using the EpiTect bisulfite conversion kit (Qiagen) following manufacturer’s instructions. Libraries were constructed using TruSeq reagents (Illumina) and sequenced on the Hiseq 2000 (Illumina).