WXS of mouse: WT untreated, female, 19194
Genomic DNA extractions from fresh frozen mouse tissues or cultured cells were performed using the DNeasy Blood and Tissue Kit (Qiagen). DNA Exome Capture library preparation was performed from 1 ľg of the extracted mouse tissue or cell culture genomic DNA using SureSelect XT Mouse All Exon (Agilent Technologies) with four cycles of pre-capture and 10 to 12 cycles of post-capture amplification. The libraries were diluted to 2 nM or 10 nM, according to their final concentration, and clustered at 10 pM using the TruSeq PE Cluster Kit v3 - cBot - HS or the HiSeq Rapid PE Cluster Kit v2. Exome libraries were sequenced either separately in one lane of a high output flowcell or as a pool of four libraries spread over two rapid flowcells v2 (4 lanes).