GSM2790147: NF54-TP2_Non-Induced; Plasmodium falciparum; RNA-Seq
RNA was isolated from 500 μL Trizol solution by three extraction steps (1x using 500 μL phenol:chloroform:isoamyl alcohol (25:24:1); 2x using 100 μL chloroform). After vigorous shaking for 30 seconds, phases were separated by centrifugation (12000g, 10min, 4 °C). RNA was purified from 200 μL of the aqueous phase using an RNeasy micro kit (Qiagen) and corresponding protocol. RNA was eluted in 32 μL DEPC-treated water, quantified by Qubit (Life Technologies) and stored at -80 °C. 0.5 μg - 1.0 μg total RNA of each sample was used for library preparation. Libraries were prepared by using TruSeq Stranded mRNA kit (Illumina). Library quantification was conducted by qPCR and TapeStation (Agilent Technologies) measurements. Libraries were subsequently pooled and sequenced using 300 cycle V2 MiSeq reagent kit (Illumina)