Exome-seq of Benzo[a]pyrene-derived Post-stasis and Immortal Human Mammary Epithelial Cells
Exome sequencing was performed using the Life Technologies AmpliseqTM Exome Kit (Cat. #4487084) according to the manufacturer’s protocols (MAN0008346).  This kit uses a pool of 293,000 primer sets divided into 12 multiplexed PCR reactions to selectively amplify exons.   In brief, 100 ng of genomic DNA was used to seed 12 exon amplification reactions of 10 cycles.  After completing PCR cycling the 12 reactions were combined prior to primer digestion and adapter ligation.  A unique barcoded adapter was used for each sample.  After adapter ligation, the libraries were purified and then quantified by qPCR.  Library concentrations were in the range of 150-600 pM.  Pairs of barcoded libraries were diluted in equimolar concentrations for template preparation using the Ion OneTouch instrument and then sequenced on a single Ion Proton P1 chip.