GSM2442844: Screening 1 LLL-Y2H; Mycobacterium tuberculosis; Mus musculus; OTHER
All the colonies on SD/-AHLT selective plates were pooled for plasmid extraction, according to Clontech yeast plasmid extraction protocol The plasmid were amplified, and then harvested for MmeI digestion.These digested products were ligated with Illumina P5 and P7 adaptors to generate a high throughput sequencing library. The libraries were constructed into the pmAD vector in yeast Y2HGoldstrain and Y187 strain by recombilation.