Pol III ChIP-seq in H1 hESCs
H1 hESCs were grown on Matrigel using mTESR for 5 days, cross-linked with 1% formaldehyde for 15 mins and harvested. Antibody against human Pol III subunit Rpc155 (from Dr.Bob White's lab) was used to perform ChIPs (as described in Oler et al., NSMB 2010). Eluates from 3 ChIPs were pooled and library preparation was carried out per Illumina's guidelines (using the TruSeq library prep kit).