GSM1647146: Patient P Blood 2; Staphylococcus aureus; RNA-Seq
RNA was isolated using the RNeasy Protect Bacteria Kit (Qiagen) according to the manufacturer instructions (protocol 4). DNase I (DNAfree kit, Ambion) was used to remove remaining DNA. The concentration of the RNA was determined by spectrophotometry via fluorometry using the Qubit Fluorometer RNA BR Kit (Invitrogen). Quality and quantity of the RNA was examined by analysis on Tapestation (Agilent Technologies). Sequencing libraries were constructed using the NEBNext Ultra Directional Library Preparation Kit for Illumina (#E7420 ,NEB)