GSM3264348: Batch1_ISOX_Biological_Replicate_1; Homo sapiens; RNA-Seq
RNA from primary human airway epithelia was isolated with the RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). Two cultures (approximately 600,000 cells total) were used for each extraction. Initial quality control was performed with Nanodrop 2000 (Thermo Fisher Scientific, Wilmington, DE, USA) and Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Library prep and sequencing performed at the Iowa Institute of Human Genetics Genomics Division. Sequencing libraries were prepared with the Illumina TruSeq Stranded total RNA ribozero gold sample preparation kit (Illumina, San Diego, CA) starting with 500 ng of input total RNA, and following Illumina's sample preparation guide.  The libraries were analyzed on the Fragment Analyzer (Advanced Analytical, Ankeny, IA) and combined in equimolar concentrations into pools. The pools were analyzed on the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) using the High Sensitivity chip to evaluate size distribution. The concentration of each pool was measured using the KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Pools were sequenced on the Illumina HiSeq 4000 sequencer with a 150 bp Paired-End SBS chemistry (Illumina) across three lanes.