Oligodendrocyte Precursor Cells Transcriptome, Full-length Iso-seq, rep1, 1-2 kb fraction
Synthesis of full-lenth cDNA was performed on RNA isolated from cultured cells by using SMARTer PCR cDNA Synthesis kit.  For each sample, three cDNA fractions were obtained with BluePippin and sequenced at the RSII Instruments using 2 SMRT cells for the 1-2 kb fraction, and 3 SMRT cells for 2-3 kb and 3-6 kb fractions, to a total of 8 SMRT cells per sample.