GSM2610635: Cdx2-/- Villus PBRM1 ChIP-seq biological rep1; Mus musculus; ChIP-Seq
Whole cell extracts from villi fixed with 2 mM DSG and 1% formaldehyde were sonicated and incubated overnight with Dyna beads (Thermo Fisher Scientific) conjugated with 5 ug PBRM1/BAF180 antibody (Bethyl Laboratories, A301-591A) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.