Illumina sequencing of DNA viruses from tomato in Kenya
Libraries were prepared using Nextera DNA kit. Genomic DNA was normalized to 2.5ng/l followed by enzymatic fragmentation. Tagmentation was done and barcoded using indexed adapters and cleaned with AMPure XP magnetic beads. Quality and quantity of the library was confirmed with the Agilent Tape Station 2200 system and QubitTM fluorometer.