GSM1464883: Central pedicel; Malus domestica; RNA-Seq
mRNA was isolated from 50 μg of total RNA using a FastTrack MAG Micro mRNA Isolation Kit (Life Technologies, http://www.lifetechnologies.com/) following the manufacturer’s protocol. Following the quantification of the isolated mRNA using Agilent RNA 6000 Nano LabChip, 200 ng of mRNA were used to generate cDNA libraries with cDNA Rapid Library Preparation Kit (Life Technologies). The cDNA libraries were used for emulsion PCR as described by the manufacturer. Following assessment of cDNA quality using an Agilent 2100 BioAnalyzer, pyrosequencing was performed on a Roche 454 GS-FLX sequencer.