DNA-Seq of Arabidopsis thaliana: single seedling
DNA was isolated using a Plant DNeasy Kit (Qiagen) following the manufacturers protocols and then processed according to the Truseq Nano DNA Library Prep Kit and protocol (Illumina). Fragmentation was performed by Covaris ultrasonication and an enrichment step of 7 cycles of PCR was performed.  Library validation included size and purity assessment with the Agilent 2100 Bioanalyzer and the Agilent DNA1000 kit (Agilent Technologies). DNA libraries were pair-end sequenced with an Illumina HiSeq2000