Thermophilic Digestion System 2 Timepoint 4 (day 122)
Microbial community DNA was extracted from the anaerobic digester sample using a CTAB-based, indirect lysis protocol, and then 16S amplicons were generated with the Takahashi et al. primers Pro341F (5'-CCTACGGGNBGCASCAG-3') and Pro805R (5'-GACTACNVGGGTATCTAATCC-3'). Illumina Nextera XT indexes were incorporated onto the amplicons, and sequencing was performed using a MiSeq Reagent Kit v3 (600 cycle).