GSM1643703: HS01_CD3_U1; Homo sapiens; RNA-Seq
Total RNA was prepared from sorted cells by using an AllPrep DNA/RNA Micro Kit (Qiagen), and then treated with DNase I and then bound to Dynabeads Oligo(dT) EcoP magnetic beads. The double-stranded cDNA synthesized on the beads was digested with Nla III and ligated to the adaptor containing an EcoP15I restriction enzyme recognition site. The adapter-ligated cDNA was digested with EcoP15I to release the tag containing the adaptor sequence and 27 bp of unique sequence from a single transcript. SAGE-seq libraries were prepared for sequencing using SOLiD SAGE Kit with Barcoding Adaptor Module and multiplexed by using SOLiD RNA Barcoding Kit Module 1-16 according to the manufacturer’s instructions.