GSM3040612: Kr[If-1] No Phenotype Total mRNA Biological Repeat 1; Drosophila melanogaster; RNA-Seq
Total RNA was isolated from 10 heads using TRIzol (Thermo Fisher Scientific) and mRNA sequencing was performed as described elsewhere (J Biol Chem 292, 6039-6046). RNA integrity was verified on an Agilent 2200 TapeStation (Agilent Technologies, Palo Alto, CA). 100-200 ng of total RNA was used to prepare RNA-Seq libraries using the TruSeq RNA Sample Prep kit following the protocol described by the manufacturer (Illumina, San Diego, CA). Please note that polyA containing RNA was first purfied prior to the library preparation step. Single end sequencing (1X50 cycles) was performed on an Illumina HiSeq2500. Sequencing was performed at Hollings Cancer Center Genomics Core Lab at MUSC. RNA libraries were prepared using TruSeq RNA Sample Prep Kit after selecting for polyA RNA