Illumina NovaSeq 6000 paired end sequencing; ChIP-Seq for TCF7L2 in adult mouse thalami
ChIP-Seq for TCF7L2 in adult mouse thalami
Fresh thalami isolated from adult mice were cut briefly and fixed with 1% formaldehyde for 10 minutes, chromatin isolated according to Cotney and Noonan, 2015 was sonicated into 200-500 bp fragments with Covaris S220 DNA was purified with Monarch PCR & DNA Cleanup Kit (T1030, NEB) Library preparation was conducted by NGS Core Facility, Centre of New Technologies, University of Warsaw. Libraries were prepared with KAPA HyperPrep Kit and KAPA Dual-Indexed adapters (Cat. no. 7962363001, KK8722 Kapa Biosciences). Enrichment by 15 cycles of amplification was applied and final library was size-selected using Kapa Pure Beads to obtain avarage size of 350 bp. Libraries were tested for quality with High Sensitivity DNA kit (Cat. no. 5067-4626, Agilent)