NextSeq 500 paired end sequencing; RNAseq of murine small intestinal tissue following epithelium-specific deletion of Bcl9/9l +/- Apc +/- mutant B-catenin
RNAseq of murine small intestinal tissue following epithelium-specific deletion of Bcl9/9l +/- Apc +/- mutant B-catenin
Small intestine of mice was isolated, flushed with water. Approximately 7cm from the end of proximal small intestine (stomach end), 0.5cm of tissue was isolated and then transferred to RNA alter and stored at -80 until processing Whole pieces of small intestinal tissue were used for RNA purification using RNeasy Mini Kit (QIAGEN, #74104) according to the manufacturer's instructions. RNA integrity was analysed with a NanoChip (Agilent RNA 6000 Nanokit #5067-1511). A total of 2µg of RNA was purified via Poly-A selection. TruSeq RNA Library Prep Kit v2