GSM4455959: C2C12 Control Cells RNA-seq 2; Mus musculus; RNA-Seq
Total RNA was isolated using an RNeasy Kit (QIAGEN) using an on-column DNase digestion (RNase-Free DNase Set, QIAGEN) to avoid genomic DNA contamination. Library preparation and 126-bp paired-end RNA-seq was performed by the Next-Generation Sequencing Facility at The Centre for Applied Genomics in The Hospital for Sick Children. RNA integrity was assessed using the Bioanalyzer platform (Agilent Technologies, Inc.). Sequencing was performed using standard procedures for  the Illumina HiSeq 2500 platform.