Sampling pit U3_4-10
The libraries were created according to Rozanov, A. S., Bryanskaya, A. V.,Ivanisenko, T. V., Malup, T. K., & Peltek, S. E. (2017). Biodiversity ofthe microbial mat of the Garga hot spring. *BMC evolutionary biology*, *17*(2),254.DNA extracted from the samples was used as a template for amplification ofbacterial and archaeal 16S rRNA genes with universal primers: 343F (50-CTCCTACGGRRSGCAGCAG)  806R (50-GGACTACNVGGGTWTCTAAT). NGS sequencing ofthe variable V3-V4 regions of the 16S rRNA gene was performed on 237 MiSeq(Illumina) using the MiSeq reagent kit v.2 (Illumina). Library preparationwas done with 238 Nextera DNA sample prep (Illumina). Sequencing wasperformed at the Faculty of 239 Bioengineering and Bioinformatics,Lomonosov Moscow State University in April 2013.