Total RNA from liver of TiLV-infected Nile tilapia
Library was prepared from 100 ng total RNA without any enrichment of mRNA or rRNA depletion, using TruSeq RNA library preparation according to the manufacturer's protocol (Illumina Corporation USA). Library was amplified and barcoded using 15 cycles of PCR. Library quality and quantity were checked using a D1000 screentape (Agilent) and qPCR (NEB) to enable equimolar pooling.