GSM3772640: Control replicate 2; Gallus gallus; RNA-Seq
RNA isolation protocol: The RNA from the samples was extracted using the Norgen's Single Cell RNA Purification Kit (catalog no. 51800, Norgen Biotek Corp.). A Nanodrop© spectrophotometer (ND-1000 Nanodrop Technologies, Wilmington, DE, USA) was used to assess the initial RNA purity and concentration of each sample. To ensure adequate RNA quantity for analysis, we pooled nine GD region samples (8μl from each) per replicate. We created 5 pooled replicates per treatment group, for a total of 15 replicates. The RNA integrity of each replicate was assessed on an Agilent 2100 Bioanalyzer instrument (Model 62939B, Agilent Technologies, Santa Clara, CA, USA) at the Georgia Genomics and Bioinformatics Core (GGBC) at the University of Georgia (Athens, GA). RNA-Seq for the Illumina' TruSeq strand-specific kit.