Geneva 41 Chicken litter treatment, replicate no. 1
Rhizosphere soil was removed using a brush from apple rootstock roots. DNA was extracted using Mobio's Power Soil DNA Isolation Kit. 16S rRNA gene was amplified using the 515f/806r primers from (Caporaso et al., 2011). Reverse primer contained the golay barcode. Pooled barcoded samples were sequenced on Illumina MiSeq instrument using the 500 cycle v2 kit.