GSM2254356: wild type RNA-seq repeat2; Oryza sativa Japonica Group; RNA-Seq
The total RNA samples of 3-5mm root tip were isolated using TRIzol reagent (Invitrogen) from wox11 mutant and corresponding wild type Hwayoung, which were 5 days after germination. The total RNA was used to generate Illumina sequencing libraries following the manufacturer's protocol. RNA libraries were prepared according to the Illumina RNA-seq library construction protocol strictly.  Briefly, 4 μg of total RNAs was used for mRNA purification, first strand cDNA systhesis, second strand cDNA systhesis, 3' end of blunt double strand cDNA ligated with "A" nuleotide, index primers ligation, low cycle PCR amplyfication, and double-strand DNA purification.