Silene vulgaris flower transcriptome, low elevation garden, high elevation genotype GAS
Three rounds of selection with Sera-Mag Oligo(dT) Magnetic Particles (Thermo Scientific) were used to enrich for mRNAs. These samples were then used for RNA-seq library construction following standard Illumina protocols with the NEBNext mRNA Library Prep Reagent Set (New England Biolabs) to make 2x100 PE libraries. All 16 libraries (8 flower, 8 leaf) were multiplexed using Illumina barcodes, balanced, and run on two lanes of an Illumina HiSeq2000 by Genewiz, Inc.