GSM3886281: Con A2; Homo sapiens; RNA-Seq
Total RNA was extracted using the Trizol reagent (Life technology) according to the manufacturer's protocol. And rRNAs were removed using Ribo-Zero rRNA Removal Kits (Illumina, USA). RNA libraries were prepared for sequencing using standard Illumina protocols and sequencing library was determined by Agilent 2100 Bioanalyzer using the Agilent DNA 1000 chip kit (Agilent, part # 5067-1504) according to the manufacturer's protocol.