Illumina HiSeq 3000 paired end sequencing; Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells
Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells
We generated RNH1 knock out K562 cells using the CRISPR/Cas9 method. Total RNA was isolated from wild type and RNH1 deficient K562 cells. Illumina TruSeq Stranded mRNA protocol Illumina TruSeq Stranded mRNA protocol. We prepared Illumina TruSeq stranded mRNA libraries. The libraries were subjected to a preparative size selection on a PippinHT instrument to exclude library molecules with insert sizes of less than 300 bp.