Sequencing experiment of Sample 2
Libraries of small RNAs were prepared from 5ug total RNA using Illumina’s Small RNA Alternative v1.5 Sample Prep Protocol with the following modifications.  Adapter ligation times were increased from 1 hour to 6 hours, a total of 15 PCR cycles were used, and a 10% acrylamide gel was used for better resolution of properly ligated sequences from unligated free adapters. Sequencing of adapter libraries was performed on an Illumina GAiix using version 5 chemistry and RTA version 1.8.70.0.  Both runs were 36-cycle single read.  Raw sequencing data was post-processed using CASAVA 1.7.0.