GSM2790466: Sample_3869_B22; Homo sapiens; RNA-Seq
Total RNA was isolated from the formalin-fixed paraffin-embeded (FFPE) specimens using the RecoverAll kit for FFPE with extended proteinase K and DNase treatement (Life Technologies Inc., Carlsband, CA) Double stranded cDNA preparation and library construction was done with the Ovation Human FFPE RNA-sequencing Multiplex System (NuGEN, San Carlos, CA) using 200ng total RNA.