GSM2011117: B6_H3K36me3_ChIPSeq; Mus musculus; ChIP-Seq
Nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. After high-speed spin soluble chromatin was removed and used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.