Soil samples were collected from 15-30 cm and soybean nodules collected from lateral roots during the V6-8 growth stages.  Dna was extracted from the soil and soybean nodules.  Soil DNA was amplified using nosZ and amoA primers with adaptors to target denitrifying bacteria, and ammonia oxidizing bacteria and archaea, respectively. Nodule DNA was amplified using nifH and nodZ primers to target nitrogen fixation and soybean specific Bradyrhizobium.  A second PCR reaction was used to insert a unique barcode to this sample