DNA-seq of Amazonian environmental samples from insects
Samples from Slam-trap opened 24h; DNA extracted using lysis buffer; PCR targeting the V7 region (~110 bases) of the 18S rRNA gene using the forward and reverse primers (5'-TTTGTCTGSTTAATTSCG-3') and (5'-TCACAGACCTGTTATTGC-3') of eukaryote; Macrogen companie amplification and Illumina MiSeq sequencing