Control 48 hour North Shore Channel river water
River water was collected from North Shore Channel at Touhy Ave. near the surface (0-1m depth), 100 ml incubated in the lab in the dark for 48h, and passed in succession through ~1.6 m pore size glass fiber filter for pre-filtration and captured on a 0.22 m pore size polycarbonate membrane filter for collecting free-living prokaryotic component. RNA was extracted using phenol:chloroform based extraction, and used for RT-PCR amplification of 16S rRNA gene with primers 27F/534R. PCR products were submitting for sequencing in paired-end format. This library represents one of the duplicates for this time-point.