GSM1462765: Drosha KD #2 (48h MSC treatment); Mus musculus; RNA-Seq
Total RNA from kidney was extracted using TRIzol (Invitrogen). RNA extraction was performed following manufacturer protocol. For RNA-Seq library preparation, approximately 2 μg of TRIzol (Invitrogen) isolated total RNA from whole kidney was subjected to polyA+ selection and library preparation using the TruSeq RNA Sample Prep Kit (Illumina), following manufacturer instructions