RNA-Seq of Apis mellifera pupa injected with WT virus USA 2015, time 0
A pink-eye honeybee pupa from a Florida brood frame with low Varroa infestation and which had no observable Varroa mite feeding exposure, was intra-abdominally injected into the hemolymph to introduce 5 microgram of wild-type RNA extracted from a DWV virus particle preparation (see BioSample SAMN08430537). Total RNA was extracted immediately after injection (time 0) for Illumina TruSeq library preparation and sequencing.