WGS of Canis familiaris: B-cell lymphoma  (G1-FineNeedleAspirate)
Samples from non-neoplastic  tissue (blood mononuclear cells) and lymphoma (fine needle aspirate samples  were collected from either the prescapular, submandibular, or popliteal lymph  node) were collected at the time of diagnosis. DNA was isolated using the  DNeasy Blood and Tissue kit (Qiagen, Valencia, CA)  following the manufacturers protocol. Sample quantity and purity were  assessed with a NanoDrop spectrophotometer (260/280 > 1.46, 260/230 >  0.45) and Qubit fluorometric quantitation. Samples were sent to McGill  University and Gnome Qubec Innovation Centre, Montral, QC, Canada, for  Illumina HiSeq X Ten PE150 sequencing with qPCR and using  Illumina TruSeq DNA v3 adaptors.