5- whole metagenome
Water samples were collected, and filtered through glass fiber filter using vacuum pump and DNA were extracted from the filters. Extracted DNA was prepared and processed for sequencing using the Nextera XT kit (Illumina, San Diego, CA) according to the manufacturers instructions. After processing, libraries were assessed for size using an Agilent TapeStation 2000 automated electrophoresis device (Agilent Technologies, Santa Clara, CA) and for concentration by a Qubit flurometer (Thermo Fisher Scientific Inc., Waltham, MA). Libraries were pooled in equimolar ratio and sequenced on a mid-output kit using an Illumina NextSeq500 sequencer, with paired-end 2x150 base reads.