RNA-Seq of chronic wound sample
Samples were stored in RNAlater until prepared for sequencing. Cells were lysed by bead beating, and total RNA was extracted with RNA-Bee (Tel-Test). Ribosomal RNA was depleted with the Ribo-Zero Gold rRNA Removal Kit (Epidemiology) (Epicentre). Remaining RNA was fragmented with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Libraries were prepared with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs). Finished libraries were run on a 10% Mini-PROTEAN TBE gel (BioRad) and size-selected for fragments ~140-300bp.