GSM2949457: Group3: 4T1 Clone#3 rep2 (miR-204-5p); Mus musculus; RNA-Seq
Total RNA from 4T1 overexpressing miR-204-5p or control was purified using TRIzol reagent (Thermofisher, USA). A TruSeq RNA library preparation kit (Illumina, San Diego, CA, USA) was used according to the anufacturer's instructions. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100-nt paired-end sequencing by Illumina HiSeq2500