GSM2011217: DGM-12251-LA-BC; Homo sapiens; RNA-Seq
Total RNA from harvested nonsmoker basal cells was extracted with TRIzol with subsequent RNA clean-up using the RNeasy MinElute RNA pu-rification kit to remove residual DNA The TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) was utilized as per protocol to generate cDNA that was then purified with QIA-quick PCR purification kit (Qiagen, Valencia, CA) Samples were loaded onto an Illumina flowcell for paired-end 125 x 2 sequencing reactions using the Illumina HiSeq 2500, using 4 samples multi-plexed per lane.