TruSeq of mixed microorganisms from 0.2 um filtered fresh urine sample collected in portable urinal potty during a three-days public event
Urine sample was collected and filtered using 0.2 micron polycarbonate membrane filter in a vacuum pump.  Then DNA extraction was conducted from the filter using DNA extraction kit (Maxwell) with slight modification in the initial extraction step. After measuring the quantity of DNA in cubit fluorometer, 100 ng DNA was submitted to the DNA Sequencing Core of the University of Michigan for library preparation, cluster generation and sequencing.