GSM3884921: WT rep2 (cecum); Vibrio cholerae; RNA-Seq
Luminal samples from the ileum and cecum of 3-day old infant rabbits infected with V. cholerae were snap-frozen in liquid nitrogen for subsequent phenol:chloroform RNA isolation. Contaminating DNA was removed using the DNA-free kit (Applied Biosystems) and RNA was stored at -80°C. The Ovation Complete Prokaryotic RNA-Seq Library Systems (NuGEN) was used for cDNA synthesis and library preparation of 200 bp (mean size) fragments. cDNA library quantification was performed using the Agilent 2200 TapeStation.