GSM1171594: H2-2; Bombyx mori; RNA-Seq
Silkworm hemolymph was collected by cutting the first and second pairs of prolegs from the fifth instar day-5 larvae, using the sterile tubes containing a few crystals of phenylthiourea. Hemolymph samples were centrifuged (4000 x g, 10 min, 4℃) and the supernatants were stored at -80℃ until use. The silkworm hemolymph was extracted using mirVana™ PARIS™ Kit (AM1556, USA). RNA libraries were prepared for sequencing using standard Illumina protocols