Metagenomic Sequencing of Hot Springs from Central India
The shotgun metagenomic libraries were prepared using Illumina Nextera XT sample preparation kit (Illumina Inc., USA) using the manufacturer’s protocol and were evaluated on 2100 Bioanalyzer using High Sensitivity DNA kit (Agilent, USA) to estimate the library size. Libraries were quantified using Qubit dsDNA HS kit on a Qubit 2.0 fluorometer (Life technologies, USA) and KAPA SYBR Fast qPCR Master mix and Illumina standards and primer premix (Kapa Biosystems, USA) as per the Illumina suggested protocol. Equal concentration of libraries were loaded on Illumina NextSeq 500 platform using NextSeq 500/550 v2 sequencing reagent kit (Illumina Inc., USA) and 150X2 bp paired-end sequencing of the libraries was performed.