Illumina sequencing of Linum usitatissimum genome (cultivar Atlant)
DNA was fragmented on an S220 ultrasonic homogenizer (Covaris, USA), and then 1 g of DNA was used for the library preparation by a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK) with a size selection of adaptor-ligated DNA of about 600-800 bp.