GSM1647965: Ce_V2 [miRNA-Seq]; Homo sapiens; miRNA-Seq
Peripheral blood samples (10ml) were collected into EDTA tubes that were frozen for DNA preservation, and Tempus RNA tubes (Life Technologies, Grand Island NY) for preservation of RNA. Total RNA was isolated from Tempus tubes using the Life Technologies Tempus Spin RNA Isolation Kit following manufacturer's protocols. Small RNA libraries were prepared using Illumina TruSeq Small RNA Sample Prep kits from total RNA following the manufacturer’s protocols.