GSM2702293: Bird N. crassa 240min rep 2; Neurospora crassa; RNA-Seq
RNA was isolated from homogenized mycelia with TRI REAGENT (Invitrogen) and mRNA was purified using Dynabeads oligo(dT) magnetic separation (Invitrogen) Twenty-four sequencing libraries, 3 replicates per condition, were produced from purified total RNA samples by the Illumina TruSeq stranded protocol. The libraries underwent 76bp pair-end sequencing using Illumina HiSeq 2500 according to Illumina protocols.