WGS of Homo sapiens: iPSC: Sample R1_LP6005344-DNA_A01
"The Qiagen DNeasy Blood and Tissue Kit (cat. no. 69504) was used to prepare genomic DNA from ~2 million cells of each cell line, as recommended by the manufacturer. Template DNA fragments were hybridized to the surface of flow cells (Genome Analyzer Paired-End Cluster Kit (v2) or HiSeq Paired-End cluster Kits (v2.5 or v3)) and amplified to form clusters using the Illumina cBot. Paired-end libraries were sequenced for 2 _ 101 (HiSeq) or 2 _ 120 (GAIIx) cycles of incorporation and imaging using the appropriate TruSeq SBS kits. Each library or sample was initially run on a single GAIIx lane for quality control validation, and further sequencing was performed on either the GAIIx (?4 lanes) or HiSeq (?1 lane) platform."