Hi-C Seq of mus musculus: adult male spleen
Resting B cells from lncCSR KO mouse spleen were isolated by negative selection using CD43 magnetic beads (Miltenyi Biotec) and cultured in RPMI1640 containing 15% FBS with 20 g/ml LPS (Sigma-Aldrich), 20ng/ml IL4 (PeproTech) and 1ng/ml TGF (R&D) for 24 hours. Five million cells were collected for Hi-C experiment. Hi-C was performed following in situ Hi-C protocol (Rao et al., Cell 2014) and modified. Restriction enzyme: DpnII