Induced_sample
The mRNA was isolated from the total RNA of T. kiharae induced sample using RNA purification beads followed by fragmentation and priming for cDNA synthesis as recommended by the manufacturer (Illumina, USA). For double-stranded cDNA synthesis, the SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, USA) was used, further purification was accomplished using Agencourt AMPure XP beads (Beckman Coulter, Inc, USA). End repairing and 3-end adenylation were performed following the RNA adapter ligation. Upon enrichment of DNA fragments library templates were validated using Agilent 2100 Bioanalyzer (Agilent, USA). Clonal clusters were created from DNA library templates using TruSeq PE Cluster Kit v2 and cBot automated system (Illumina, USA). Clusters obtained were used to carry out paired-end runs by Genome Analyzer IIx (Illumina, USA). Illumina HiSeq2000 sequencing was carried out on the equipment of EIMB RAS Genome Center.