ChIP-Seq of mus musculus
ChIP immunoprecipitation was performed using a PierceTM Agarose ChIP Kit (Catalog number:  26156, Thermo USA). The antibody used to capture Pur-alpha was purchased from SANTA CRUZ (sc-130397). HT22 cells were seeded in a 10 cm diameter petri dish and subjected to ChIP immunoprecipitation with cell growth to 95% confluence. The ChIP immunoprecipitation operation was carried out according to the instructions. The main processes included formaldehyde cross-linking, sonication, enrichment of the target protein with the magnetic beads coated with the antibody, de-crosslinking, and recovery of the DNA fragment. The library was constructed using the ChIP-seq Library Prep Master Mix Set for Illumina (NEB, USA), the main processes including end repair, addin A to the 3end, ligate adaptation, gel purification and size selection, PCR amplification. Sequencing was performed using the Illumina HiSeq 2500 sequencing platform.