GSM3963593: E175_BP_KO_1; Mus musculus; RNA-Seq
Total RNA was isolated from FAC-sorted GFP positive cells from Hes5::GFP; Ski KO and Tbr2::GFP; Ski KO transgenic lines using TRIzol reagent. A time course was performed with NSCs, BPs and NBNs isolated at each time point during development from E10.5 to E18.5. Samples were analyzed for their integrity and concentration using Agilent 2100 Bioanalyzer and Quant-IT RiboGreen RNA Assay Kit. Sequencing libraries were prepared with the Illumina TruSeq RNA Library Prep Kit v2 according to Illumina's instructions. After quality control (Fragment Analyzer, AATI) libraries were pooled and loaded on an Illumina flow cell for cluster generation (HiSeq SR Cluster Kit v4 cBot). Libraries were sequenced SR50 on the HiSeq 2500 system (HiSeq SBS Kit V4) following the manufacturer's protocols.