GSM3963133: 10X+0X (2); Homo sapiens; OTHER
Forty-eight hours after transfection, total RNAs were extracted by RNAiso Plus (TaKaRa, Beijing, China) reagent. cDNAs were synthesized using the PrimeScript TM RT reagent Kit with gDNA Eraser (TaKaRa) following standard protocol. KOD-Plus-Ver.2 DNA polymerase (Toyobo) was used to amplify the corresponding cDNA sequences surrounding ldsDNA junctions. Library construction and high-throughput sequencing were conduct by Novogene (Beijing, China). Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer's recommendations and index codes were added. The library quality was assessed on the Qubit Fluorometer and Agilent Bioanalyzer 2100 system. At last, the libraries were sequenced on an Illumina HiSeq 2500 platform and 250 bp paired-end reads were generated.