RMC5T
RNA was extracted from fresh frozen tissue samples using the RNeasy Kit (Qiagen) according to the manufacturers instructions. After controlling for the quality of the initial samples, rRNA depletion was performed for the total RNA for each sample, followed by random-primed and stranded cDNA preparation and quality control. Total RNA was converted into a library of template molecules for sequencing on Illumina HiSeq2000, with a paired-end read length of 100 to 125 nt. The quality of the FASTQ reads was evaluated using the FastQC software.