GSM2703980: DAH4; Drosophila melanogaster; RNA-Seq
Heads and thoraxes of frozen flies were separated on dry ice, mixed and immediately used for RNA isolation. Total RNA was isolated using RNeasy Microarray Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions. Eluted RNA was treated with DNase I (Thermo Scientific) and concentrated by ethanol precipitation. All RNA samples of OR and DAH were prepared simultaneously Library preparation and indexing were performed from 1 ug of total RNA using the TruSeq RNA sample prep v2 kit (Illumina) according to the manufacturer's instructions