GSM1462581: wtb; Rattus norvegicus; RNA-Seq
The RNA, extracted with the RNeasy Mini Kit (Qiagen, Valencia, CA) from two, carefully washed clones of PC12, the wtPC12 and the hrPC12 previously referred to as PC12-27 [24,25,29-31], was analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) Libraries, prepared starting from 2 mg of RNA/sample with the Illumina TruSeq RNA Sample Prep kit v2 procedure, were quantified by the Qubit BR assay (Life Technologies, Illkirch, France) and the Agilent 2100 Bioanalyzer, and sequenced on the Illumina HiSeq 2000 platform