fungal community 28S rDNA sequences
This library represents fungal 28S rRNA sequences amplified from DNA extracted from a high-lignin substrate, collected after 18 months of decomposition in plots 1 and 2 of our study site.  Plots 1 and 2 are ambient N deposition plots in our field experiment. We amplified these sequences using fungal primers LR0R (ACCCGCTGAACTTAAGC) and LR3 (CCGTGTTTCAAGACGGG).  We used 10 barcode pairs for multiplexing of these samples: 1) gcgctctgtgtgcagc/agagtactacatatga, 2) tatacatacacgcact/tagtgcagatcgatgt, 3) tatctatcgtatacgc/atgtatctcgactgca, 4) cactagctctgactac/tctgtacgtagctcat, 5) ctgctagagtctacag/cgagctatctcatact, 6) acgacatgatactgct/tctcactatgatacgt, 7) agagcatctctgtact/tgtcgcatcatatgat, 8) cgacgtatctgacagt/cgcgtgcagagtgtca, 9) catacgctgtgtagca/ctactgtctcgcactg, 10) cactgatcgatatgca/gtacactagtgcacat.  Barcode pairs 1-5 were used for plot 1 samples.  Barcode pairs 6 - 10 were used for plot 2 samples.