"Rapid and robust phylotyping of spa t003, a dominant MRSA clone in Luxembourg and other European countries.  "
"DNA samples were prepared for multiplexed, paired-end sequencing on the GAIIx Genome Analyzer  (Illumina, Inc, San Diego, CA) following the manufacturers protocol.  For each isolate, 1-5?g dsDNA in 200?l was sheared and then purified using the QIAquick PCR Purification kit (Qiagen). Enzymatic processing of the DNA followed the guidelines as described in the Illumina protocol, but enzymes for processing were obtained from New England Biolabs (New England Biolabs, Ipswich, MA) and the oligonucleotides and adaptors were obtained from Illumina. After ligation of the adaptors, the DNA was run on a 2% agarose gel for 2 hours, after which a gel slice containing 500-600 bp fragments of each DNA sample was isolated and purified using the QIAquick Gel Extraction kit (Qiagen). Individual libraries were quantified with qPCR on the ABI 7900HT (Life Technologies Corp., Carlsbad, CA) using the Kapa Library Quantification Kit (Kapa Biosystems, Woburn, MA). Based on the individual library concentrations, equimolar pools of 12 to 24 S. aureus libraries were prepared at a concentration of at least 1nM. "