RNA-Seq of Entylia carinata: 5th instar wingpads
5th instar wingpads were dissected from individuals preserved in RNALater. Tissues were pooled with brood-mates (4 ind. in pool C). RNA was extracted and polyA enriched with Sera-Mag Oligo (d)T beads. Sequencing libraries were prepared with the KAPA Stranded RNA-Seq kit, using New England Biolabs adapter and primer. This is a stranded library using the (d)UTP method.