HTS platform comparison 454 FLX data
two step-pcr approach for amplicon library generation; quantitation of single amplicons with Quant-it PicoGreen dsDNA Assay Kit; dilution of amplicons to 1 x 10^9 molecules and equimolarly pooling; preparation of a working dilution of 1x 10^7 molecules for downstream application; emPCR was performed with the GS FLX Titanium SV emPCR Kit (Lib-A) including emulsification, amplification, bead recovery, and DNA library bead enrichment, followed by the sequencing reaction according to the standard protocols. SFF files containing read base calls and per base quality scores were used for further analysis.