Seawater microbiomes collected from Laoshan Bay, the Yellow Sea, China
Seawater samples were collected at the euphotic zone from six stations in May, August, November 2013 and March 2014 at Laoshan Bay, the Yellow Sea, China. DNA was extracted from the cells collected on filter using E.Z.N.A.® Water DNA Kit (OMEGA Bio-Tek, USA). Bacterial amplicons targeting the V1-V3 hypervariable region (Escherichia coli positions 5-534) of the SSU rRNA gene were generated using 40 ng of DNA as template and barcoded primer pairs 27F (5'- NNNNNNNN-AGAGTTTGATCCTGGCTCAG -3') and 533R (5'- NNNNNNNN-TTACCGCGGCTGCTGGCAC -3'). Equal amount of amplicons from all samples were pooled and sequenced using a Roche 454 Titanium pyrosequencer (Branford, CT, USA). The barcodes are listed below.  #SampleID BarcodeSequence  1-May AGACATG  4-May AGAGTGC  7-May AGAGCTA  10-May AGTACAG  11-May AGTTCCT  16-May AGTCTAC  1-Aug AGTCGTT  4-Aug AGCAACT  7-Aug AGCATGA  10-Aug AGCTCTG  11-Aug AGCTGAA  16-Aug AGGACTT  1-Nov TACAGAG  4-Nov TAGACGA  7-Nov TAGTGCA  10-Nov TAGCTCT  11-Nov TTCAGCA  16-Nov TTGACAG  1-Mar GTGTCAA  7-Mar AAGAGCT  10-Mar AAGTCAG  11-Mar AACTGTC  16-Mar AACGTGT