GSM3040336: day3_iN_rep1; Homo sapiens; RNA-Seq
RNA lysis buffer was used to lyse cells. Each lysate was then transferred to a 1.5 ml centrifuge tube, which was then placed on dry ice for rapid freezing and then stored at -80 °C. Total RNA isolation was performed using a kit following the manufacturer's protocol. RNA-Seq libraries were prepared following the Smart-seq2 protocol. 1 ng of total RNA was used. cDNA was synthesized and then used in each NexteraXT (Illumina) reaction.