pacbio hifi low input sample for mosquito
Genomic DNA extracted from single individuals using "modified 10X protocol" (Kingan et al 2018 https://doi.org/10.3390/genes10010062). PacBio HiFi libraries were made according to the Procedure & Checklist - Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0. 230 ng genomic DNA was used for each sample which were barded with PacBio barcodes bc1020 and bc1022. HiFi reads were generated with Circular Consensus Sequencing analysis and samples were demultiplexed with Demultiplex Barcodes in SMRT Link v 9.0.0.92188.