16S rRNA gene sequencing of Mus musculus: colonic luminal content of NR mouse 2 days old
sequenced at the Georgia Genomics and Bioinformatics Core at the University of Georgia (Athens, GA, US) on a llumina MiSeq flow cell v2 with a 500 cycle (PE250) reagent kit. The V4 region of the 16S rRNA gene was amplified using the primers 515FB18 (5-GTGYCAGCMGCCGCGGTAA) and 806RB19 (5-GGACTACNVGGGTWTCTAAT)