Complete mitochondrial genome of Apis laboriosa_MZ collected from Mizoram
Standard fragment libraries represent a collection of regions of 300-600 bp insert length obtained from randomly fragmented genomic DNA. Individual single-stranded inserts function as template molecules during sequencing. Two ends of such inserts are sequenced for a length of 150 nt each read in a 150 PE sequencing run. Illumina chemistry detects bases using a sequencing by synthesis approach where a DNA polymerase inserts fluorescent-tagged bases corresponding to the template molecules. This process is carried out in a massively parallel manner to enable the sequencing of millions of insert molecules simultaneously.