GSM3775701: PB_HSC_24: CD71- PB HSC; Homo sapiens; RNA-Seq
Within CD34+ CD38- CD45RA- HSC/MPPs (purified as in Notta et al., Science 2011), we defined 2 subsets as follows:  CD71+ CD34lo cells (CD19- CD38- CD45RA- CD71+ CD34lo) and CD71- CD34hi cells (CD19- CD38- CD45RA- CD71+ CD34lo). To obtain samples for RNA-seq, subsets were purified from CD34+ enriched non-mobilised PB samples from platelet apheresis cones by FACS. 20 cells were directly sorted into lysis buffer according to the Smart-seq2 protocol published by Picelli et al., Nat. Protocol 2014. RNA libraries were prepared for sequencing using standard Illumina protocols