nosZ amplicon sequencing, corn 2010
Using environmental DNA, the catalytic subunit region of the nosZ gene was amplified using primers described in Mao et al. (and a protocol described in de Oliveira et al. (Environmental Microbiology, 2013) and a PCR protocol described by de Oliveria et al. (Veterinary Microbiology, 2013). Libraries were constructed using Lib-L kits, with 0.8 DNA molecules per bead. We pooled 15 samples per sequencing run, distinguishing amongst them with 7 bp barcodes.