16S metagenome of Rhesus 7D post-Zika virus infection: male adult rectal swab
Sequencing of the V4 region of the bacterial 16S rRNA gene was performed using the 515FB-806RB primer set. Amplified using the FailSafe PCR System in triplicate for each sample followed by pooling of 10ng of each replicate. The pooled library was cleaned using MO BIO UltraClean PCR Clean-Up Kit and quantified using the KAPA Library Quantification Kit. Sequencing was carried out as detailed in the Earth Microbiome Project protocol; specifically, 7 pM of the pooled library with 30% PhiX phage as a control was sequenced using a 300-cycle Illumina MiSeq Kit, generating 150bp paired-end reads.