GSM1646832: DN_thymocytes_rep2; Mus musculus; OTHER
Thymocytes from litters of 3 week old C57BL/6 Rag2-/- mice were crosslinked and chromatin was subject to HindIII restriction digest. Sticky ends were religated using T4 DNA ligase and DNA was purified from nuclear lysates. DNA was subsequently digested with DpnII and re-ligated with T4 DNA ligase to make 4C library templates. Inverse PCR using primers containing Illumina HiSeq adaptors was performed on 4C templates. Libraries were amplified from 4 viewpoints: TEA, INT1, INT2, and E-delta.