GSM2522443: dwetA_RNA-seq-2; Aspergillus flavus; RNA-Seq
Total RNA was isolated by adding silica/zirconium beads (Biospec, Bartlesville, OK) and Trizol (Invitriogen, San Diego) and homogenizing in a Mini Bead Beater (Biospec), and then subsequently following manufacturere's instructions (Invitrogen) Strand-specific library was prepared from total RNA using Illumina TruSeq Strand specific RNA sample preparation system.