GSM2611074: QQ rep 2; Bos taurus; RNA-Seq
Total RNA was extracted using TRIzol® Reagent (Life Technologies) according to the manufacturer’s specifications; 5μg of the total RNA was purified with the RNeasy Mini Kit (Qiagen, Inc., Valencia, CA); quality was assessed with an Agilent Bioanalyzer 2100 (Agilent Technologies); samples with RIN > 7.0 were used to construct sequencing libraries. 1μg  from each sample were used for library construction using the Illumina’s TruSeq Stranded mRNA Library Preparation Kit (Illumina); libraries were indexed, pooled and sequenced for 101 cycles on a HiSeq 2500 (single-end chemistry) using a HiSeq SBS sequencing kit version 4 (four lanes were used).