RNA-seq of CSCCs: patient 1
Total RNA was extracted from CSCC and adjacent normal tissues using Trizol reagent (Invitrogen, Carlsbad, CA, USA).  cDNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA).  Library fragment size was measured using Bioanalyzer.  Libraries were quantified using both Qubit fluorometer with Quant-iT dsDNA HS Assay kit (Invitrogen) and qPCR with KAPA library quantification kit (Cat. KK4824, Kapa Biosystems).  Equal amount of library cDNA from each sample was pooled and sequenced from both ends using Illumina Hiseq 2500 Platform (Illumina, San Diego, CA, USA).