GRO-seq of homo sapiens: nascent RNA siRAD21 + EtOH(repeat3) MCF7
The GRO-seq measures the dynamic trancription activity of nascent RNAs in the genome. MCF7 or other nuclei were extracted and were subjected to nuclear run-on for 5minutes at 30 degree with BrU labeling. The run-on RNAs were pull down by an antib-BrU conjugated agarose beads (santa Cruz biotech) and subjected to library preparation for deep sequencing.