Pol III ChIP-seq in Adipose derived stem cells (ADSCs from Invitrogen)
ADS cells were grown as per manufacturer's protocols (Invitrogen) to 90% confluence, cross-linked with 1% formaldehyde for 15 mins and harvested. Antibody against human Pol III subunit Rpc155 (from Dr.Bob White's lab) was used to perform ChIPs (as described in Oler et al., NSMB 2010). Eluates from 3 ChIPs were pooled and library preparation was carried out per Illumina's guidelines (using the TruSeq library prep kit).