ChIP-seq of homo sapiens: Pol2 siRAD21 + E2 MCF7
ChIP-seq measures the binding of specific factor to chromatin regions. The samples were done in MCF7 cells starved from hormone signaling for 3 days, then treated with either ethanol or 100nM estrodial for 1hour. The cell chromatin were crosslinked by 1% formaldehyde, then sonicated for binding by specific antibodies. The immunoprecipitated DNA-protein complexes were purified by a dynabeads G,  washed and de-crosslinked. The DNA was finally eluted using QiaQuick DNA extraction kit (QIagen). About 1-20ng of ChIP DNA was prepared into sequencing library using KAPA LTP library prep kit following the manufacturer's instrucitons.