gDNA from S.spontaneum young leaves sheared at 500 bp
Total genomic DNA was extracted from leaves using the CTAB method with minor modifications. For each library, 3-5 ug of genomic DNA was sheared using Covaris S220 Adaptive Focused Acoustics ultra sonicator. Libraries were constructed following Illumina standard protocol and sequenced at Cold Spring Harbor Laboratory on Illumina GAII machine using the paired-end 100 cycle protocol. The sequence quality was evaluated by measuring the quality of the reads according to the 90% of bases having a base quality greater or equal than 20 (Q20) using FASTX Toolkit version 0.0.6 (Hanon lab).