GSM2011455: HepG2 Control 1; Homo sapiens; OTHER
Total RNA was extracted using TRIzol reagent. RNA was then subjected to DNaseI treatment to remove any contaminating DNA. mRNA was then isolated using oligo(dT) Dynabeads (Invitrogen). Library construction was performed using the miCLIP method to map m6A residues at single-nucleotide resolution: Linder et al, Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome. Nature Methods, 2015 Aug;12(8):767-72.