Near-complete West Nile Virus genome sequencing from mosquito pools: sample VN6864CPR2_QC_MIC_101_2016_Culex
Near-complete West Nile Virus genome amplification from mosquito pools was performed using different sets of primers generating 6 overlapping RT-PCR products. All 6 products were quantified and pooled at equimolar concentrations. One nanogram of DNA of this pool was used for library preparation using the Nextera XT DNA library preparation kit (Illumina, Inc., San Diego, CA, USA) following the manufacturers instructions. Sequencing was performed on an Illumina MiSeq platform (Illumina) using a MiSeq reagent kit v2 (500 cycles, paired-end).