GSM2609237: 5C_CTCF-AID-NPC-sublcone_treatment-scheme-5_rep1; Mus musculus; OTHER
For ChIP-seq cells were  trypsinized and fixed in suspension; For Chromosome Conformation Capture cells were fixed in tissue culture plates and scrapped off; For RNA-seq cells werre washed once with PBS and lysed in Trizol in the tissue culture plate. ChIP libraries were prepared using standard Illumina protocols; RNA-seq libraries were assembled using the non-stranded kit E7530L from NEB; 5C (in-situ ligation) and Hi-C (dilution ligation) were assembled based on Illumina-compatible protocols. Detailed experimental procedures can be found in the supplementary information of Nora et al. 2017 Cell ChIP-seq, ChIP-exo, RNA-seq, Hi-C, 5C