GSM1173501: cell-9; Mus musculus; OTHER
Single cells treated according to the modified Hi-C protocol were isolated in PBS and incubated at 65 degrees C for overnight. The biotin-labelled DNA from each cell was bound to Dynabeads M-280 streptavidin magnetic beads (Life Technologies), digested with Alu I (New England Biolabs), A-tailed with Klenow Fragment (3'->5' exo-) (New England Biolabs) and dATP, ligated with customised Illumina adapter with T4 DNA ligase (New England Biolabs), then the library was amplified by PCR with library amplification primers, and size-selected between 300 and 700 bp on an agarose gel.