GSM1648109: UMSCC25_AZD8931_2; Homo sapiens; OTHER
Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. 6x10^6 cells from each of the cell lines (UMSCC25, UMSCC8, UMSCC1) were plated into duplicate 15cm plates (3x106 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication