RNA-seq of Fragariocoptes setiger
Total RNA was extracted from tissue with Trisol reagent according to manufacture instruction. Quality was checked with  BioAnalyser and  RNA 6000 Nano Kit(Agilent). PolyA RNA was purified with Dynabeads mRNA Purification Kit (Ambion). Illumina library was made from polyA NEBNext Ultra II RNA Library Prep  (NEB) according to manual. Sequencing was performed on HiSeq1500 with 250 bp read length. PCR primers with index sequences: GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG. There was one run on MiSeq-2500, Single End 250bp of sequencing, generated 82 596 682 SE reads.