Control 0 hour WWTP Effluent
Effluent was collected at Terrence J. OBrien Water Reclamation Plant. and 100ml immediately passed in succession through ~1.6 m pore size glass fiber filter for pre-filtration and captured on a 0.22 m pore size polycarbonate membrane filter for collecting free-living prokaryotic component. RNA was extracted using phenol:chloroform based extraction, and used for RT-PCR amplification of 16S rRNA gene with primers 27F/534R. PCR products were submitting for sequencing in paired-end format.