data sheet of WT_
Ovary is dissected from 1-year-old gynogenetic silver crucian carp, and total RNA is isolated and he mRNA is enriched by using the oligo(dT) magnetic beads (for eukaryotes)or by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments (about 200 bp). Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads