GSM2523014: RNA-seq_ventral_DG_enriched_environment_rep5; Mus musculus; RNA-Seq
Tissue collection consisted of rapid removal of the brain, followed by flash freezing and storage at -80C. Frozen brains were sliced coronally at 200 µm thickness until reaching bregma -2.30. The brains were then removed from mounting position, rotated, and remounted to the mounting position for horizontal slicing ventral dentate tissue. Horizontal sections were sliced from interaural 3.24 mm to 0.92 mm26. A 300 µm diameter puncher was used to punch dorsal and ventral dentate gyrus region separately. RNA and DNA extraction were performed from the same sample using Qiagen Allprep DNA/RNA Mini kit (Qiagen, Cat# 80204.).  We performed on-column DNase I treatment during RNA extraction and on-column RNaseA treatment during DNA extraction. RNA was examined by Bioanalyzer 2100 (Agilent technologies, Santa clara, USA). RNA-seq: The RNA libraries were prepared in McGill University and Genome Quebec Innovation Centre using Illumina TruSeq Stranded total RNA LT set (Cat# RS-122-2301, Illumina Canada Ulc.). Paired-end, 100bp read-length RNA-seq was collected using HiSeq 2000 at a depth of 30 M sequencing.