GSM2300010: FVB M IMQ 1; Mus musculus; RNA-Seq
Total RNA was extracted from flash-frozen skin samples using the RNeasy Fibrous Tissue Mini Kit (Qiagen, cat. no. 74704) with on-column DNase digestion (Qiagen, cat. no. 79254). RNA concentrations were evaluated using the NanoDrop spectrophotometer and RNA integrity was assessed using the Agilent Bioanalyzer. RNA was further assessed for quality using the TapeStation (Agilent, Santa Clara, CA) with manufacturer's recommended protocols. All samples had RNA integrity numbers of 8 or greater. Sequencing of fragmented complementary cDNA was performed using the Illumina Genome Analyzer Iix