Posterior_Neck
Reads are 100 nt in length. cDNA fragments are from 80bp to 600bp with an average size of 250bp. The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). The libraries were quantitated by qPCR and sequenced on two lanes for 101 cycles from each end of the fragments on a HiSeq2500  using a HiSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina). Adaptors have been trimmed from the reads. Sequence of adaptors used to make the libraries (used blue bold portion for adaptor trimming). Adaptor sequence in read1:AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG (NNNNNN= 6 nt index), Adaptor sequence in read2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT