Illumina HiSeq 2500 sequencing; Chipseq on Sox18Ragged-mCherry over-expressed zebrafish larvae at 26-28hpf
Chipseq on Sox18Ragged-mCherry over-expressed zebrafish larvae at 26-28hpf
zebrafish were maintained by Biological resources Aquatics in University of Queensland according to guidelines conformed to Queensland Animal Ethics Committee. Positive fish larvae were were collected at 26-28hpf, fixed in 1% formaldehyde for 10 mins, and quenched with 1 mol/L glycine. Fish were dissociated with a mortar and pestle in liquid nitrogen, lysed and subsequent immunoprecipitation was performed using DsRed polyclonal antibody (Clontech, #632496).  DNA amplification was performed using TruSeq ChIPseq kit (Illumina, IP-202-1012) following IP. The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KAPA Biosystems, KK4824).