GSM1447396: 5053_CD4_Naive_48 [RNA-Seq]; Homo sapiens; RNA-Seq
RNA was purified from cell pellets with TriZol following standard protocols Total RNA was converted to cDNA with the Ovation RNA-seq system (NuGEN) following standard protocols.  cDNA was S1 endonuclease digested.  cDNA libraries were end-repaired, A-tailed, and ligated to indexed adapters.  Purified library was amplified with 15 cylces of PCR followed by size selection.  Cluster generation and sequencing was conducted on an Illumina GAIIx following manufacturer's intructions.