GSM1642637: WT 22°C; Escherichia coli K-12; ChIP-Seq
Cells were resuspended in Lysis buffer (10mM tris, 20% sucrose, 50mM NaCl, 10mM EDTA, 10mg/ml lysosyme, 0.1mg/ml EDTA, 0.1mg/ml RNaseA) and subsequently incubated for 30min at 37C. Next, IP buffer was added (50mM Hepes, 150mM NaCl, 1mM EDTA, 1%triton, 0.1% sodium deoxycholate, 0.1% SDS). Cells have been sonicated, centrifugated, and the supernatent was used for the immunoprecipitation. Sequencing libraries were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (#E7370, NEB) for samples 1 to 3 (37 C) and the WaferGen PrepX ILM ChiP-Seq Library Kit (#400075, WaferGen) on the Apollo 324 System for samples 4 to 10 (22 C).