GSM3963771: P5_before; Homo sapiens; RNA-Seq
Gastrocnemius muscle samples were removed, put on ice and saved in liquid nitrogen for storage. RNA was extracted using ExtractRNA reagent (Evrogen, Moscow,Russia, Cat#BC032) and quantified with Bioanalyser 2100 instrument  (Agilent Technologies, Palo Alto, CA) using BA RNA nano kit. 3' mRNA libraries were prepared using QuantSeq 3'mRNA-Seq Library Prep Kit for Illumina (FWD) (Lexogen, Vienna, Austria, Cat#015) from 1mkg of total RNA and PCR anplified for 15 cycles. Libraries were sequenced on MiSeq using kit MiSeq Reagent Kit v3 2x75 (Cat#MS-102-3001) in the single-end read mode.