GSM3040838: MCF7_siUSP1_1; Homo sapiens; RNA-Seq
Total RNA was extracted by TRIZOL Reagent (Life Technologies, Inc) and quantified by Agilent Bioanalyzer 2100 (Agilent technologies). The cDNA fragments were subjected to an end repair process, added a single 'A' base, and ligated with the adapters. The products were then purified and enriched with PCR to create the final cDNA library.