GSM2254211: WT.Treg_RNAseq.2; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen). Libraries were prepared with Illumina TruSeq Stranded mRNA sample kits using indexed adaptors (Illumina). Pooled libraries were subjected to 75 bp paired-end sequencing according to the manufacturer’s protocol (Illumina HiSeq3000). Targeted sequencing depth was 30 million paired-end reads per sample.