Whole genome typing of Paracoccidioides sp.
About 1g of purified DNA was used for sequencing library preparation using the KAPA library preparation kit for Illumina NGS sequencing (Kapa Biosystems). Libraries were indexed with 8-bp nucleotide tags and quantified using a Kapa library quantification kit (Kapa Biosystems) on a 7900HT Instrument (Life Technologies). Libraries were sequenced to a read length of 100bp using v3 or v4 chemistries on an Illumina HiSeq 2500 instrument (Illumina, San Diego, CA) on a high output mode.