V5V6 16S amplicon sequencing of Alma Dananta pond
5 ng of metagenomic DNA extractedwere used for library preparation (methods described in C. Manzari, et al.: The influence of invasive jellyfish blooms on the aquatic microbiome in a coastal lagoon (Varano, SE Italy) detected by an Illumina-based deep sequencing strategy,Biol Invasions,2015, 17 6(3):923-940. ). Libraries were subjected to a 2_250 bp paired-end sequencing on the Illumina MiSeq platform. To increase the genetic diversity, as required by the MiSeq platform, a phage PhiX genomic DNA library was added to the mix and co-sequenced.