GSM1647862: inducible Treg (iTreg) cells, 2; Mus musculus; miRNA-Seq
RNA was harvested using Trizol reagent. Approximately 1 µg of total RNA was used for Next Generation Sequencing following the Illumina protocol, TruSeq_SmallRNA_SamplePrep_Guide_15004197_C.pdf, using 12 cycles of PCR. The prepared library was then loaded onto an Illumina HiSeq v2 single-read flowcell, standard cluster generation was performed on a Cbot and sequenced for 40 bases of the insert and 7 bases of the index read using standard HiSeq sequencing reagents. Reads were then processed using CASAVA 1.8 and demultiplexed based on index sequences.