GSM1646790: nulliparous luminal progenitor; Mus musculus; Bisulfite-Seq
Cells were incubated with cell lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCL pH 8) and DNA purified with phenol/chorophorm/isoamilic alcohol purified genomic DNA was fragmented, adenylated, and ligated to Illumina-compatible paired-end adaptors. Bisulfite conversion was performed using the EZ DNA Methylation Gold kit (Zymo) according the manufacturer’s instruction. Bisulfite converted, adaptor-ligated fragments were PCR enriched and further utilized on pair-ended Illumina sequencing.