GSM1462557: AB_rep2; Caenorhabditis elegans; RNA-Seq
Trizol-stored blastomeres were thawed and re-frozen up to three cycles to lyse them. RNA extraction was performed using a Trizol/chloroform extraction followed by RNeasy Micro (Qiagen) preparation using On Column DNaseI Digestion (Qiagen). mRNA was amplified using in vitro transcription (TargetAmp aRNA Amplification Kit, Epicentre). Standard RNA-seq protocol  (Illumina mRNA Sequencing Sample Preparation Guide, September 2009) was used to sequence amplified aRNA. Magna beads were used for final purification in lieu of gel purification.