GSM4456346: Brd4 KO_16hr_REP2; Mus musculus; RNA-Seq
RNA-seq:Total RNA was extracted using Quick-RNA Miniprep Kit (ZYMO research). Library preparation, including ligating barcode, was performed using a Mondrian SP (NuGEN Technologies Inc.) and the Ovation SP Ultralow Library system (NuGEN). Fragments ranging from 250 to 450 bp were selected and subjected to paired-end sequencing on a HiSeq 2000 sequencing system (Illumina).