GSM3040482: iN_5wk_3D_100ul_7.36mg/ml_Mtrgl_10mpml_rep2; Homo sapiens; RNA-Seq
3D culture hydrogel was dissociated and human neuronal cells were sorted from dissociated hydrogel-cell suspension into a buffer preserving RNA integrity, which was then centrifuged and then stored at -80 °C. Total RNA isolation was performed using a kit following the manufacturer's protocol. RNA-Seq libraries were prepared following the Smart-seq2 protocol. 1 ng of total RNA was used. cDNA was synthesized and then used in each NexteraXT (Illumina) reaction.