GSM3265757: PB1438Pop5; Homo sapiens; RNA-Seq
RNA was purified using QIAGEN Micro RNEasy columns, and RNA quality assessed using Agilent Bioanalyzer 2 ng of total RNA was used as input for mRNA amplification using 5' template-switch PCR with the Clontech SMART-Seq v4 Ultra Low Input RNA kit, according to manufacturer's instructions. Amplified mRNA was fragmented and appended with dual indexed bar codes using Illumina NexteraXT DNA Library Prep kits