Amplicon sequencing of soil culturable bacteria: mine substrate_replicate 4
Genomic DNA were extracted from bacterial colony suspension.   Bact341F (5-CCTACGGGNGGCWGCAG-3) and Bact785R (5'-GACTACHVGGGTATCTAATCC-3) were used. Illumina adapter overhang nucleotide sequences were added to the primer. The PCR reaction was performed thus: denaturing at 95 C for 3 min, followed by 25 cycles of denaturing at 95 C for 30 s, annealing at 55 C for 30 s and extension at 72 C for 30 secs, and a final extension at 72 C for 5 min.