GSM2611336: L-GFP+; Mus musculus; RNA-Seq
The FACS purified mouse prostate luminal GFP+ and luminal GFP- epithelial cell populations are subject to total RNA extraction by RNeasy mini kit (Qiagen, Valencia, CA). Illumina TruSeq Stranded Total RNA Preparation Kit (Illumina, cat#: RS-122-2301) was used with 400~1000 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols