WGS (Illumina) of Durio zibethinus: Musang King fruit stalk (D1)
The short read genomic library was prepared using the TruSeq Nano DNA Library Preparation Kit (Illumina) in accordance with the manufacturer’s recommendations. Briefly, 1 ug of extracted intact genomic DNA was sheared (Covaris), with 200 – 300 bp insert size, and adapter ligated. The DNA fragments were subjected to PCR cycles, and the DNA library was used in paired-end sequencing (2x100 bp, ~750 M paired-end reads, ~202X coverage) using the Illumina Hiseq 2500 sequencer (Illumina).