DNA sequencing of Aureococcus anophagefferens CCMP1794
We obtained genomic DNA from the Provasoli-Guillard National Center for Marine Algae and Microbiota. Ten micrograms of DNA were sonicated 2x2 min. on power setting 2.5 of a Misonix 3000 water bath sonicator at room temperature. The sheared DNA was cleaned up and size selected by first binding it to 0.7 volumes of Agencourt AMPure XP beads (Beckman Coulter) and collecting the supernatant. That supernatant was then diluted and mixed with 0.75 volumes of Agencourt AMPure XP beads, this time washing and eluting the bound DNA. Two hundred nanograms of the purified and size-selected DNA were made into a library without PCR by using the Encore Rapid Library System (NuGEN; this kit has since been renamed the Ovation Rapid DR System). Paired-end 100 base sequencing was performed.