TMPAB_ACID_AMP
Barcoded amplicon sequencing (bTEFAP ) was done for identification of 16S rRNA genes, PCR primers 515/806 were used with barcode on the forward primer. A 30 cycle PCR was setup for DNA amplification under the following conditions: Denatuartion at 94 C for 3 minutes, annealing at 94 C for 30 seconds (28 cycles), 53 C for 40 seconds, 72 C for 1 minute and finally extension for 72 C for 5 minutes.  All samples were pooled together and purified. DNA library was prepared from purified PCR products by following Illumina TruSeq DNA library preparation protocol.