GSM2254467: mESC_stranded_RNASeq; Mus musculus; RNA-Seq
RNA from mES cells was extracted using an RNeasy kit (Qiagen, USA). Polyadenylated RNA was isolated using Oligo dT beads (Invitrogen, USA). Illumina Truseq stranded and unstranded mRNA library prep kits (Illuminca, USA) were used for deep sequencing library preparation from 6ug of total RNA according to the manufacturer's protocol.