Transcriptome profiling of Paramphistomum cervi isolated from Sheep rumen
Our study represents the first detailed analysis of Paramphistomum cervi transcriptomes, with biologic replicates, generated by RNA-seq technology. Live adult parasites were collected, flash frozen on dry ice, and RNA was extracted using TRIzol* reagent)/ RNeasy* mini kit. mRNA was isolated from the total RNA using Roche mRNA isolation kit. The cDNA library was prepared using the Ion Total RNA-Seq Kit v2RNA protocols. Sequencing was carried out using Ion Personal Genome Machine* (PGM™) System (Version 3.0) along with Ion PGM™ Sequencing 300 Kit and Ion 316™ Chip.