The transcriptome of Xingzi 9104 at the seedling and boot stage
Triticum aestivum cv. XZ and Pst pathotype CYR32 were used in the study. For the inoculation, the second leaf of wheat seedling plants at the two-leaf stage and the flag leaf of wheat adult plants at the boot stage were simultaneously inoculated with fresh Pst race CYR32 with a paintbrush until whole surface became wet without run-off. Control plants (mock-inoculated plants) were treated with sterile water. Inoculated and control leaves were collected at 0, 24, 48 and120 hpi for RNA isolation. The total RNA was isolated using lysis buffer from the RNeasy Plant Mini Kit according to the manufacturer’s instructions (QIAGEN, Hilden, Germany). First-strand cDNA was synthesized with 2.5 μg of total RNA using the Reverse Transcription System (Promega, Madison, WI, USA) following the manufacturer’s directions. The RNA of two samples (Ak-M-0 and Sk-M-0) was subjected to RNA-Seq analysis at the Beijing Genomics Institute (BGI; Shenzhen, China). The RNA of six samples (Ak-M-24, Ak-M-48, Ak-M-120; Ak-I-24, Ak-I-48 and Ak-I-120) was prepared for DGE library construction and sequencing at the Beijing Genomics Institute.