GSM3405379: 4C-FGF9-Hindlimb-E15-Wt-To; Talpa occidentalis; OTHER
4C-seq libraries were prepared following the published protocol (van de Werken et al., 2012). NlaIII was used for the first digestion, and DpnII was used in the second digestion. For each viewpoint, a total of 1.6 mg of each library was amplified by PCR with the following primers: Sall1-4C-Fwd:TCAGTGGGCTGACATTTTA, Sall1-4C-Rev: TCAGTGGGCTGACATTTTA, Fgf9-4C-Fwd:CAAAAGAAACTTCCAAAACC, Fgf9-4C-Rev:CAAAAGAAACTTCCAAAACC Samples were sequenced with Ilumina Hi-Seq technology according to standard procedures. 4C-seq