GSM2950666: ER-464195-01 3; Mus musculus; RNA-Seq
Intestines were removed, flash frozen on dry ice, and total RNA was extracted from colon of mice using ISOGEN II Reagent (Life Technologies). 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs), and converted to Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols