PacBio RS II sequencing of SAMD00082707
DNA was extracted using a PowerSoil DNA Isolation Kit (MO BIO). DNA was purified using AMPure XP (Beckman Coulter). DNA was fragmented using g-TUBE (Covaris). DNA library was constructed based on a manual of 20kb Template Preparation Using BluePippinTM Size-Selection System (15-kb Size Cuttoff) (PacBio Sample Net-shared Protocol) by using SMRTBell Templete Prep Kit 1.0 (Pacific Biosiences). Size selection of DNA libraly was performed using BluePippin DNA Size Selection System (sagescience) with a parameter 10 kb. The quality of DNA libraly was evaluated by Advanced Analytical Technologies Fragment Analyzer.