GSM2790326: 20-I-R3; Saccharomyces cerevisiae; FAIRE-seq
Cell subpopulations removed using a spatula at 3 days, FAIRE samples (but not input samples) cross-linked with formaldehyde, DNA sheared by sonication, phenol-chloroform extraction carried out to purify DNA fragments FAIRE and input DNA samples were prepared for sequencing using ThruPLEX reagents (Rubicon Genomics, Ann Arbor, MI). 2-5 ng FAIRE or input control DNA was used as starting material for library preparation. Sequencing was performed on a NextSeq 500 instrument (Illumina, San Diego, CA) with 75 bp single-end reads.