RNA-Seq of Homalodisca vitripennis: 5th instar second thoracic segment legs
Second thoracic segment legs (middle legs) were dissected from 5th instar individuals preserved in RNALater. Tissues were pooled between two individuals for pool A.  RNA was extracted and polyA enriched with Sera-Mag Oligo (d)T beads. Sequencing libraries were prepared with the KAPA Stranded RNA-Seq kit, using New England Biolabs adapter and primer. This is a stranded library using the (d)UTP method.