Input for adipose derived stem cells (ADSCs) ChIP-seq.
ADSCs were grown as per manufacturer's protocols (Invitrogen) to 90% confluence, cross-linked with 1% formaldehyde for 15 mins and harvested. Chromatin was isolated as described (Oler et al., NSMB 2010) and input used for IPs was purified to use as a control. Library preparation was carried out per Illumina's guidelines (using the TruSeq library prep kit).