GSM2790171: Control RNASeq 5; Homo sapiens; RNA-Seq
Total RNA was extracted using Direct-zol RNA Miniprep kit (Zymo Research) according to manufacturer’s indications We use poly-A pull-down to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required) and proceed on library preparation by using Illumina TruSeq. Libraries are then sequenced using Illumina HiSeq2500/HiSeq4000 at Columbia Genome Center. RNA prep kit. Libraries are then sequenced using Illumina HiSeq2500/HiSeq4000 at Columbia Genome Center. We multiplex samples in each lane, which yields targeted number of single-end/paired-end 100bp reads for each sample, as a fraction of 280-400 million reads for the whole lane.