Mycobacterium lepraemurium genome sequencing
DNA was extracted from isolated bacteria grown in Balb/c mice. The DNA was sheared in a Covaris g-TUBE (Covaris, Woburn, MA, USA) to obtain 10 kb fragments. After shearing the DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies, Ames, IA, USA). 4 ľg of the sheared DNA was used to prepare a SMRTbell library with the PacBio SMRTbell Template Prep Kit 1 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturer's recommendations. The resulting library was size selected on a BluePippin system (Sage Science, Inc. Beverly, MA, USA) for molecules larger than 8 kb. The recovered library was sequenced on one SMRT cell with P6/C4 chemistry and MagBeads on a PacBio RSII system at 240 min movie length.