GSM2301455: 86.2_15_Ctrl; Homo sapiens; RNA-Seq
Flash frozen LuCaP PCa xenograft tissue samples were histologically evaluated for regions of viable tumor.  RNA was isolated from 16 animals (2 control, 2 treated from each line) with 10-micron sections from xenografts with ≥80% tumor content taken.  For xenografts with <80% tumor, stroma and necrotic tissue were removed prior to sectioning.  RNA was extracted using the Qiagen RNeasy Kit, (Qiagen Inc.), according to the manufacturer's protocol.  On-column DNase digestion was performed.  RNA concentration, purity, and integrity was assessed by NanoDrop (Thermo Fisher Scientific Inc) and Agilent Bioanalyzer. RNA-seq libraries were constructed from 1 mg total RNA using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit according to the manufacturer’s protocol. Barcoded libraries were pooled and sequenced on the Illumina HiSeq 2500 generating 50 bp paired end reads.