17 months amplicon of amended Australian coal well
Archaeal 16S rRNA genes were amplified with the universal archaeal primers Archf349 (5’- GYGCASCAGKCGMGAAW-3’) and Archr789 (5’-GGACTACVSGGGTATCTAAT-3’) (Klindworth et al., 2013) for 30 cycles. PCR products were used for library preparation and sequencing steps according to the manufacturer’s instructions (Life Technologies, Carlsbad CA, United States).