GSM1646361: P47_WT_HFSC_1; Mus musculus; RNA-Seq
Back skin is dissected and treated with Collagenase and Trypsin to achieve single cell suspension. Stem cells are purified through FACS by antibody staining and gating of H2BGFP(+), Sca-1(-), CD34(+) and CD49f(+) cells. NEBNext® Ultra™ Directional RNA Library Prep Kit