Silene vulgaris flower transcriptome, high elevation garden, high elevation genotype GAS
Three rounds of selection with Sera‐Mag Oligo(dT) Magnetic Particles (Thermo Scientific) were used to enrich for mRNAs. These samples were then used for RNA‐seq library construction following standard Illumina protocols with the NEBNext mRNA Library Prep Reagent Set (New England Biolabs) to make 2x100 PE libraries. All 16 libraries (8 flower, 8 leaf) were multiplexed using Illumina barcodes, balanced, and run on two lanes of an Illumina HiSeq2000 by Genewiz, Inc.