GSM2610064: HSR-RNA-GBM1+VitC-RNA-2; Homo sapiens; RNA-Seq
Total genomic RNA were extracted from cultured cell pellets using the RNeasy and QiaShredder Kits (Qiagen) according to manufacturer’s protocols . The DNA samples were treated with 3 μL of RNase solution (Qiagen) and Proteinase K Solution (20 μg/μL; Qiagen) at 65 °C for 10 minutes. RNA libraries were prepared for sequencing using standard Illumina protocols