GSM4456375: IF_neg_8; Homo sapiens; RNA-Seq
hGLU-Venus organoid samples showing Venus expression were dissociated to single cells, and stained with DAPI and DRAQ5. Live cells (DAPI-, and DRAQ5+) were FACS sorted into 2x populations (Venus+, and Venus-). RNA extraction and isolation were performed using RNAeasy Micro Plus kit (Qiagen). RNA concentration and quality (RIN 6-9) were assessed using RNA6000 Pico Kit and Bioanalyser 2100 (Agilent). The library was constructed with 2 ng of input RNA using the SMARTer Stranded Total RNA-Seq v2 Pico Input Mammalian kit (Takara Bio).