Case5
DNA extracted from FFPE after Proteinase K digestion by phenol/chloroform extraction. Multiplex library preparation was performed using the Ovation Ultralow Library System  (NuGEN, San Carlos, CA, p/n 0331-32) according to the manufacturer’s specifications with 200 ng of sample DNA. Hybridization-capture of pooled libraries was performed using custom-designed bait libraries (Nimblegen SeqCap EZ Choice, p/n 06588786001) spanning ~1.8 Mb of the genome including exons and introns 13-16 of MET.