RNA-seq of control colonocytes after 4 hours (3rd replicate)
Poly-adenylated mRNAs were isolated from thawed cell lysates using the Dynabeads mRNA Direct Kit (Ambion). RNA-seq libraries were prepared using a protocol modified from the NEBNext Ultradirectional (NEB) library preparation protocol to use Barcodes from BIOOScientific added by ligation. The libraries were then pooled and sequenced on two lanes of the Illumina Next-seq 500 in the Luca/Pique laboratory using the high output kits for 75 cycles and 300 cycles to obtain paired-end reads.