GSM2791409: CXIR60OC4-matched normal mucosa (NM); Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instruction. Briefly, 700 μL of QIAzol was added to samples and homogenized with a TissueLyser LT (QIAGEN) for RNAlater immersed tissues or by vortexing for 1 minute for cultured cells. After incubation of the homogenate for 5 minutes at room temperature, 140 μL chloroform was added and centrifuged at 12,000g and at 4°C for 15 minutes. Thereafter, transfer the upper aqueous phase to a new tube, and total RNA was extracted and eluted in 60 μL of RNase-free water using QIAcube (QIAGEN). Illumina TruSeq V2 kit