large_insert library
By using ultrasound the genomic DNA was interrupted into fragments as certain length (500bp and 6000bp ). And then, the cohesive ends of these DNA fragments were repaired by T4 DNA ligase into the blunt ends, and an A base was added at the 3-terminal ends that was ligated with special linkers with a T base at the 3-terminal ends. Next, the PCR product amplified from these DNA fragments was used to construct library for sequencing by Illumina HiSeq 2000.