HTS platform comparison PGM IonTorrent data
two step-pcr approach for amplicon library generation; Ion Torrent sequencing was performed according to the Ion Torrent standard workflow. Using the Agilent 2100 Bioanalyzer quantitation results, all barcoded-amplicons were diluted to 26 pM and equimolarly pooled. 25 µl of amplicon library was used for downstream application using the Ion PGM Template OT2 400 Kit (MAN 00007218) for use with the Ion OneTouch 2 System. This procedure resulted in the generation of template-positive Ion PGM Template OT2 400 Ion Sphere Particles. After enrichment of the particles, a sequencing run was performed with the Ion 318 chip V2 based on the Ion PGM Sequencing 400 Kit User Guide (MAN 00007242). FASTQ-files format was generated with Torrent suite software 3.6.