Site A: matK
Pollen was sampled ever other day from May 5th to May, 11th and pooled by collection site.10% subsamples of each sample were then homogenized and bead beater pulverization was used to free DNA for extraction. Following DNA extraction, universal primers were used to co-amplify the ITS2, matK and rbcL regions. The resulting amplicon libraries were multiplexed and sequenced using the Illumina MiSeq platform.