GSM1166107: ABRF-ILMN-RIBO-D-3; Homo sapiens; RNA-Seq
NA Ribo-Depleted; Following ribo-depletion, all recovered RNA was processed using the Illumina TruSeq RNA Sample Preparation Kit v2 protocol at the “elute-fragment-prime” step, followed by the standard TruSeq protocol.  Completed libraries were evaluated by DNA quantitation and Bioanalyzer analysis, and then submitted to a single core laboratory site for sequencing.  Sequencing libraries were constructed with barcodes to allow multiplexing of 12 samples per lane, pooled to target 200 million clusters per channel and 100 million reads per library, and distributed over multiple channels of three flow cells to normalize for lane and run variability.  Sequencing was carried out on Illumina HiSeq 2500 instruments using protocols HCS 1.5.15.1 and RTA 1.13.48.