Stranded RNA-Seq of Beta vulgaris: Leaf
Total RNA was isolated from c. 70-125 mg leaf tissue collected in liquid nitrogen using the RNeasy Plant Mini Kit (Qiagen) following the manufacturers protocol. A DNase digestion step was included with the RNase-Free DNase Set (Qiagen). Quality and quantity of RNA were checked using the 2100 Bioanalyzer (Agilent Technologies). Library preparation was carried out using the TruSeq Stranded Total RNA Library Prep Plant with RiboZero probes.