GSM3265139: WH101-4.0; Lactococcus lactis; RNA-Seq
Total RNA was extracted using the RNAprep pure Bacteria Kit (TIANGEN, Beijing, China) in accordance with the instructions. Purified RNA was quantified by using NanoDrop ND-2000 (Thermo Scientific™, MA, USA). The RNA samples were stored at -80 oC before transcriptome analysis. The samples were sent to BGI (Shenzhen, China) for transcriptome sequencing. rRNA removing, mRNA purification and fragmentation, cDNA synthesis, adapter ligation and then PCR amplification were performed to construct the cDNA library. The sample library quality was examined using Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System, respectively.