GSM4456357: YF2 scATAC-seq; Homo sapiens; ATAC-seq
Nuclei extraction from sorted cells with lysis buffer (10 mM Tris-HCl (pH 7.4), 5 mM MgCl2, 5 mM NaCl, 1% BSA, 0.1% Tween-20, 0.1% NP-40, 0.01 % Digitonin. Wash with buffer (10 mM Tris-HCl (pH 7.4), 5 mM MgCl2, 5 mM NaCl, 1% BSA, 0.1% Tween-20) then wash with Nuclei buffer (10x Genomics), and resuspension in Nuclei buffer (10x Genomics) Loading was setup to target the recovery of 5'000 nuclei. The library preparation was performed using the Chromium Single Cell ATAC kit (v1.0 Chemistry) strictly following the 10X guidelines (CG000168, revB) running 12 cycles for the GEM incubation step (linear amplification) and  12 PCR cycles for the sample index PCR step.