GSM2702460: DNAM-1- NK subsets from Normal Spleen; Mus musculus; RNA-Seq
Sorted DNAM-1+ and DNAM-1- NK cells were harvested and resuspended in 350ul RLT and 3.5ul β-Me. Total RNA was exacted by QIAGEN RNeasy Mini Kit (CAT 74103). 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard BGISEQ-500 protocols.