GSM3263475: Control_2; Mus musculus; RNA-Seq
Cells were scraped and collected and RNA harvested using TRIzol standard protocol. RNA was further purified using QIAGEN RNeasy kit. Intact poly(A) RNA was purified from total RNA samples (100-500 ng) with oligo(dT) magnetic beads and stranded mRNA sequencing libraries were prepared as described using the Illumina TruSeq Stranded mRNA Library Preparation Kit (RS-122-2101, RS-122-2102).