heavy chain antibody sequences from 121 weeks post-HIV-infection
gene specific primers targeting IGHV4-39 and constant region primers specific for IgG and IgA (targeting the CH1) were used to PCR amplify relevant antibody sequences. All PCRs were don't 7 times and pooled per time point. Each time-point was then indexed using Illumina Nextera indexes and all time-points pooled and loaded onto an Illumina Miseq