RNA-seq of rice:  caryopses with middlel nitrogen  fertilizer appplication (1N: 150 kgha-1)
The caryopses of 10 days after flowering (DAF) for each sample were harvested between 10:00 and 11:00 a.m, and then immediately freezed in liquid nitrogen. Finally, caryopses at an accurated and consistent development stage were further stored in -80  refrigerator for RNA sequencing. The sequencing experiment was set up with two biological replicates, each biological replication pooled the labeled caryopses from a similar position of main panicles of 5 plants.