GSM2791477: 6w.M.C4; rat gut metagenome; OTHER
Stool samples were collected and subjected to bacterial genomic DNA (gDNA) extraction using a QIAamp stool DNA extraction kits (Qiagen) following the manufacturer’s protocol. PCR amplification was performed using primers targeting the V3 to V4 regions of the 16S rRNA gene with extracted DNA. For bacterial amplification, barcoded primer of 341F (5’-TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-CCTACGGGNGGCWGCAG-3’; underlined sequence indicates the target region primer) and 805R (5’-GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-GACTACHVGGGTATCTAATCC-3’; underlined sequence indicates the target region primer). Pyrosequencing (Illumina Miseq system; Macrogen, Inc., Seoul, Korea)