GSM1643944: VC_KO_2; Mus musculus; RNA-Seq
Visual Cortex was dissected from 8-10 week old MeCP2 knockout mice (Mecp2tm1.1Bird) or wild-type littermate controls. RNA was trizol and extracted using Qiagen Rneasy purification with on column DNase steps. ERCC control RNAs were spiked into the samples with equal mass added per visual cortex (note: they were not used in subsequent analysis). Illumina library construction and sequencing was carried out as follows: total RNA was depleted of ribosomal RNA using the Ribozero rRNA removal kit (Epicentre), heat-fragmented to 200-700 bp in length and cloned using Uricil-N-Glycosylase-based strand-specific cloning. cDNA fragments were sequenced using an Illumina HiSeq 2000 Strand-specific, rRNA depleted total RNA, Single-end 49 bp sequencing