Acyrthosiphon pisum alfalfa biotype poolseq
DNA from each individual genotype was extracted from four asexual females of the same clone using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer instructions. The 60 DNA solutions were dosed using Pherastar and pooled in equimolar proportions after RNase treatment and a paired-end library was constructed using the Genomic DNA Sample Preparation Kit (Illumina, San Diego, CA).