25 months bacterial amplicon of amended Australian coal well
Amplification of the V3-V4 region of the bacterial 16S rRNA gene was performed using universal primers Bac F341 (5’-CCTACGGGNGGCWGCAG-3’) and Bac785R (5-‘GACTACHVGGGTATCTAATCC-3’) (Klindworth et al., 2013) for 25 cycles. PCR products were used for library preparation and sequencing steps according to the manufacturer’s instructions (Life Technologies, Carlsbad CA, United States).