GSM2607978: ChIP restimulated cytotoxic T lymphocytes (Nfatc1/A-Bio.Rosa26BirA); Mus musculus; ChIP-Seq
Chromatin from 1x10^7 fixed CTLs was prepared using a lysis buffer containing 1% SDS and sonicated by a Vibra-Cell VCX 130 before precipitation of chromatin by streptavidin beads Sequencing libraries were prepared using 7ng decrosslinked and purified DNA employing the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® according to the manual (incl. size selection and 15 cycles of final PCR). All quality controls were done using Invitrogen’s Qubit HS assay and library size was determined using Agilent’s 2100 Bioanalyzer HS DNA assay. Barcoded libraries were onboard clustered usingHiSeq® Rapid SR Cluster Kit v2 using 8pM and 50bps were sequenced on the Illumina HiSeq2500 using HiSeq® Rapid SBS Kit v2 (50 Cycle). The raw output data of the HiSeq was preprocessed according to the Illumina standard protocol