GSM4296889: Input DNA of HTS; Homo sapiens; ChIP-Seq
Nuclei were isolated from formaldehyde crosslinked HTS cells. Then Lysates were clarified from sonicated nuclei and chromatin fragments were immunoprecipitated with anti TEAD4 antibody. The ChIP-Seq was performed using the Illumina NovaSeq 6000 Sequencing System at the University of Kansas Medical Center – Genomics Core (Kansas City, KS). Fragmented input and immunoprecipitated chromatin (20ng) was used to initiate the TruSeq ChIP Sample Prep Kit library preparation protocol (Illumina cat#IP-202-1012). The fragmented chromatin underwent end repair and 3' adenylation prior to illumina indexed adapter ligation. Library sizing (target size – 320bp) was performed using the Sage Pippin Prep automated size selection system. PCR amplification of the sized library fraction was performed using Illumina adaptor specific priming with final library purification using KAPA Pure magnetic bead purification (KAPA cat#KK8002).  Following Agilent TapeStation 4200 QC for sizing of the library preparation and library quantification using the Roche Lightcycler96 with Roche FastStart Essential Green Master qPCR kit (Roche cat#06402712001 ) and KAPA Library Quant (Illumina) DNA Standards (KAPA cat#KK4903), the CHiP-Seq libraries were adjusted to a 2.125nM concentration and pooled for multiplexed sequencing.