GSM1165496: Erythroblast_RNA_R2; Mus musculus; RNA-Seq
RNA was extracted from sorted fetal liver progenitor cells and erythroblasts. RNA-Seq libraries were generated and sequenced using Illumina adapters for paired-end 51-bp sequencing on the Illumina Genome Analyzer IIxplatform. Fetal livers were dissociated to single- cell suspension and stained with anti-CD71–FITC and anti-Ter119–PE antibodies (BD Biosciences PharMingen). Cell populations were isolated using a FACSAria flow cytometer running FACSDiva 6.1.3 software (BD Biosciences). Cells were collected as erythroid progenitors (Ter119-CD71- and Ter119-CD71+) or erythroblasts (Ter119+CD71+). At least 3 indepen- dent cell sorts for each population were performed.