GSM2789673: MSN LV-DKIN-K543M n1 (K1); Homo sapiens; RNA-Seq
Medium was remove and 350µl RLTplus was add, Sample were extracted on QUIACUBE following the Rneasy Plus Micro kit protocole (Qiagen) The Ion Ampliseq Transcriptome Human Gene expression kit was used with 50ng of mRNA for the construction of sequencing libraries following the manufacturer protcol. Ampliseq Transcriptome Human gene expression kit (Termofisher Scientific)