RNA-Seq of Apis mellifera pupa injected with PBS
A pink-eye honeybee pupa from a Texas brood frame with low Varroa infestation and which had no observable Varroa mite feeding exposure, was intra-abdominally injected into the hemolymph to introduce 5 microgram of control PBS buffer. Total RNA was extracted immediately after injection (time 0) for Illumina TruSeq library preparation and sequencing.