GSM2610659: Wild-type Villus H3K4me3 MNase ChIP-seq biological rep1; Mus musculus; ChIP-Seq
Villus chromatin digested with MNase was incubated overnight with 5 ug H3K4me3 antibody (Abcam, ab8580) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.