RNA-Seq of N. tabacum plants (stem section): biological replicate 1
Total RNA was extracted with 1 mL of ExtractRNA Reagent (Evrogen, Russia) and the subsequent procedures were performed according to manufacturers instructions. Residual DNA was eliminated by treatment of RNA samples with DNAse I using the DNA-free kit (Ambion). RNA quantity was analyzed using a Qubit fluorimeter (Invitrogen). For RNA-Seq, total RNA (3 g) was processed using Ribo-Zero rRNA Removal Kit (Plant) (Illumina) and then ScriptSeq Complete Kit (Bacteria)Low Input (Illumina), which included bacterial rRNA depletion step, according to manufacturers instructions.