GSM1647875: ChIP-seq pcf11-13 24C; Saccharomyces cerevisiae; ChIP-Seq
Lysates were clarified from 1% formaldehyde fixed, sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP DNA was end-repaired, A-tailed and adaptor ligated before amplification and size selection. Prepared libraries after quality control were multiplexed and single-end sequenced over one "rapid" run on HiSeq sequencer.