GSM3964013: 528_4_CSAA_week_4; Rattus norvegicus; RNA-Seq
see Tolbol, K. S. et al. Disease Progression and Pharmacological Intervention in a Nutrient-Deficient Rat Model of Nonalcoholic Steatohepatitis. Dig Dis Sci, doi:10.1007/s10620-018-5395-7 (2018). RNA quality and concentration was measured using the Fragment Analyzer from AATI (now Agilent) with the total RNA Standard Sensitivity protocol. The Sequencing library was prepared from 200 ng total RNA with the TruSeq® Stranded mRNA LT-Set B (RS-122-2102, Illumina Inc., San Diego, CA) producing a 275bp fragment including adapters in average size. In the final step before sequencing, seven individual libraries were normalized and pooled together using the adapter indices supplied by the manufacturer. Pooled libraries have been clustered on the cBot Instrument from Illumina using the HiSeq® 3000 GD-410-1001 3000/4000 SR Cluster Kit (Illumina Inc., San Diego, CA). Sequencing was performed as 85bp single-end reads and 7 bases index read on an Illumina HiSeq3000 instrument at a sequencing depth of approximately 60 million reads per sample using FC-410-1001, HiSeq® 3000/4000 SBS Kit (50 cycles, Illumina Inc., San Diego, CA).