Japanese Eel Transcriptome, fertilized Egg from Ocean
Total RNA of the whole body for each sample was extracted using Trizol® Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Purified RNA was quantified using a ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA), and characterized using a Bioanalyzer 2100 with RNA 6000 labchip kit (Agilent Technologies, Santa Clara, CA, USA) in order to confirm RIN (RNA Integrity Number) >7. A sequencing library of all the samples was constructed by Illumina TruSeq RNA Sample Prep Kits v2 (Illumina, San Diego, CA, USA) as per the manufacturer’s instructions, and the samples subsequently sequenced by Illumina HiSeq 2000 (Illumina).