GSM2254870: E2A ChIP-Seq total thymocytes; Mus musculus; ChIP-Seq
E2A-Bio bound DNA was isolated with Streptavidin coupled beads and purified through phenol-chloroform extraction. About 5 ng of ChIP-precipitated DNA was used as starting material for the generation of single-end sequencing libraries as described by Illumina’s ChIP Sequencing sample preparation protocol. DNA fragments of 200–350 bp were selected. Completed libraries were quantified with the Agilent Bioanalyzer dsDNA 1000 assay kit and Agilent QPCR NGS library quantification kit. Cluster generation and sequencing was performed by using the Illumina/Solexa Genome Analyzer II and IIx systems according to the manufacturer’s guidelines.