Typical industrial fermentation - 10 hours of fermentation
Total RNA of 13 fermentation time-points was extracted using phenol and chloroform  protocols. RNA-seq libraries were prepared from 1 *g total RNA following the manufacturer`s protocol (Illumina). Briefly, mRNA was isolated using oligo(dt) magnetic beads and fragmented in the presence of divalent zinc ions. Fragmented RNA was then used for first and second strands cDNA synthesis. Double-stranded cDNA was end-repaired and 3` adenylated for ligation of sequence adapters. After ligation of adapters, fragments of approximately 250 bp were isolated by gel electrophoresis and PCR amplified. Libraries were validated on an Experion DNA chip (Bio-Rad) and quantified using a Qubit fluorometer (Invitrogen). Each RNA-seq library was sequenced in one lane of an Illumina Genome Analyzer IIx sequencer, which produced ~20-30 million reads (36 bp single-end).