GSM2300701: Jurkat_WT_mRNAseq_rep1; Homo sapiens; RNA-Seq
Genomic DNA and total RNA from the same infected cell pool were extracted by the AllPrep DNA/RNA Mini Kit. Total RNA was further proceeded to purify mRNA by Oligotex mRNA Mini Kit. The wild-type Jurkat mRNA-seq was prepared from 100 ng total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101) according to  manufacturer's instructions with 4.5 minutes fragmentation time. Libraries were sequenced on the HiSeq2000 sequencer (Illumina) with 50 bp single end using v3 sequencing chemistry.