GSM2702394: GDC555d41; Homo sapiens; RNA-Seq
Total cellular RNA was isolated from snap-frozen xenograft tumor pieces using the TRIzol Plus RNA Purification Kit (Life Technologies).  RNA quantity and quality were assessed on a NanoDrop spectrophotometer (Thermo Scientific) and the Agilent 2100 Bioanalyzer (Agilent Technologies), respectively. RNA-Sequencing (RNA-Seq) libraries were prepared using a “total” RNA-Seq approach. Briefly, RNA preparations (1 ug) were depleted of rRNA using Ribo-Zero rRNA removal beads and indexed RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Sample Prep Kit (Illumina Inc., San Diego, CA) according to the manufacturer’s standard protocol.