GSM3964169: First instar larval brain cells, Starvation condition (scRNAseq) 1; Drosophila melanogaster; RNA-Seq
Dissected brains in PBS were dissociated into single-cell suspensions by an enzimatic treatment with collagenase. mRNAs were obtained by applying 10X Genomics protocol for scRNAseq, Chromium Single Cell 3' Library & Gel Bead Kit v3. Libraries were constructed accoring to the manufacturer's guideline, for sequencing on a Illumina NovaSeq 6000 Libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v3 (10X Genomics)