RNA-seq of Ab
Total RNA was isolated from cells of previous sampling points, using the RNeasy Mini kit (Qiagen, USA), according to the manufacturer¡¯s instructions. All procedures for RNA sequencing and alignment of the transcriptome were conducted by Beijing Berry Kang Biotechnology Co., Ltd (Beijing, China). The RNA was subjected to a subtractive Hyb-based rRNA removal process using the MICROBExpress Bacterial mRNA Enrichment Kit (Ambion).