GSM2521729: S2 T1 ATAC-seq rep2; Drosophila melanogaster; ATAC-seq
[ATAC-seq] 5x10^4 S2 cells from each time point were collected and washed in cold PBS. ATAC-Seq libraries were prepared as described previously (Buenrostro et al. Nat. Methods 2013; Buenrostro et al. Curr Protoc Mol Biol. 2015). Briefly, cells were resuspended in cold lysis buffer and centrifuged to collect nuclei. Transposition reaction was performed for 30 minutes at 37°C using Tn5 transposase from Nextera. DNA fragments were purified using a Minelute kit (Qiagen). DNA fragments were amplified using Nextera barcoded primers as described. Amplified library was purified with SPRI beads. Paired End 50 sequencing was done on an Illumina HiSeq2000 according to manufacturer's instructions.