PolyA-RNA from liver of TiLV-infected Nile tilapia
PolyA-RNA was isolated from 1 g total RNA. Library was prepared using TruSeq RNA library preparation according to the manufacturer's protocol (Illumina Corporation USA). Library was amplified and barcoded using 15 cycles of PCR. Library quality and quantity were checked using a D1000 screentape (Agilent) and qPCR (NEB) to enable equimolar pooling.