GSM2024714: Old muscle 2; Mus musculus; miRNA-Seq
For tissue samples, total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.For serum samples, total RNA was purified from 300 ul of serum samples using the miRNeasy serum/plasma kit (Qiagen) according to the manufacturer’s instructions. 2μg of total RNA from each tissue samples and 10 ng of total RNA from each serum samples were used for small RNA cDNA library preparation (26). Using Illumina TruSeq Small RNA sample preparation kit, barcoded 3'-adapters and 5’-adapters were ligated to RNA in each sample. Synthesized cDNA amplified up to 15 cycles using indexes for multiplexing. Amplified DNAs were selected by the excision (145-160bp) on 10% TBE polyacrylamide gel and purified.