GSM2254112: ESC12; Homo sapiens; RNA-Seq
sinlge cell RNAseq protocol: The Fluidigm C1 requires at least 1000 cells to be loaded (5 ml), of which about 10% fill the 96 wells of the 5-10mm diameter chip. Capture efficiency was briefly evaluated and then the chip was loaded onto the C1 for cDNA preparation using Clontech SMARTer kit (Takara-Clontech, CA). Cell debris or clusters of 2 or more cells were excluded. Hence, RNA libraries for the remaining cells were generated using the low throughput Nextera XT SNA library prep kit (Illumina, CA). Individual bar-coded libraries were sequenced by the University of Pennsylvania Functional Genomic Core using Illumina HiSeq 2500