Illumina HiSeq 2500 paired end sequencing; Regional differences in left ventricular shear stress contribute to heterogenity of the endocardial transcriptome
Regional differences in left ventricular shear stress contribute to heterogenity of the endocardial transcriptome
"Endothelial cells were obtained from adult pigs immediately after euthanasia at a local slaughterhouse. The left ventricle was dissected revelaing the chamber lumen. The ventricle was rinsed with ice-cold PBS. Endothelial cells were freshly harvested by gentle scraping of regions (1-2 cm2) located at the apex, the posterior mid-ventricle (midv) and the outflow tract (OT), just beneath the aortic valve. Endothelial cells were obtained from adult pigs immediately after euthanasia at a local slaughterhouse. The left ventricle was dissected revelaing the chamber lumen. The ventricle was rinsed with ice-cold PBS. Endothelial cells were freshly harvested by gentle scraping of regions (1-2 cm2) located at the apex, the posterior mid-ventricle (midV) and the outflow tract (OT), just beneath the aortic valve. " Total RNA was isolated by mirVana miRNA Isolation Kit (Life Technologies). The quality of RNA was evaluated by NanoDrop 1000 Spectrophotometer and TapeStation 2200 (Agilent Technologies). TruSeq RNA Sample prep library construction