DNA-seq of Amazonian environmental sample
Slam-trap opened by 24h; DNA extracted with lysis buffer followed by PCR targeting COI region of ~313 bases using an internal forward primer (5' - GGWACWGGWTGAACWGTWTAYCCYCC - 3') and the COI degenerate reverse primer (5' – TAAACTTCAGGGTGACCAAARAAYCA - 3') of metazoa; Macrogen company amplification and Illumina MiSeq sequencing.