Hydroides elegans 6 day larvae 30 min MACs rep 3
mRNA was isolated from 1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries from larval samples were prepared with NEBNext Ultra RNA Library Prep kit (NEB #E7530) and the adult library was prepared using NEBNext Ultra Directional RNA Library Prep kit (NEB #E7420) according to manufacturer’s instructions. Libraries were quantified with Qubit (Invitrogen) and the insert size distribution was assessed with 2100 BioAnalyzer (Agilent).