Single cell RNA-seq of Mus musculus retinal ganglion cells
Retinal ganglion cells were classified into distinct subtypes based upon their electrophysiological functions, following whole-cell patch recordings.  A newly fire-polished glass electrode was used to aspirate the soma and transfer it to a nuclease-free PCR tube containing TCL Buffer (Qiagen) supplemented with 1% -mercaptoethanol (Sigma).  The tubes were briefly spun on a tabletop microcentrifuge and immediately frozen at -80C for up to two weeks before processing.  cDNA was generated from each single cell using SMART-seq. Samples were pooled and sequenced on an Illumina 2500, with an average read length of 100bp. The resulting transcriptomes were analyzed using the Tuxedo Suite pipeline.