GSM1464819: I/11 cells 96 h after infection with anti-Plcg1 shRNA #133; 24h on differentiation medium; Mus musculus; MBD-Seq
Genomic DNA was isolated using Qiagen DNA mini kit. 3 μg of genomic DNA were sonicated in a Covaris S sonicator. DNA fragments which were about 150  bp in size were then subjected to enrichment with 60 μg MBD2-Fc protein in the presence of a salt gradient. The DNA fractions highly enriched for methylated DNA (eluted with 1 M NaCl) were subjected to next-generation sequencing. NEBNext ChIP-seq Library Prep Reagent Set for Illumina