GSM3776467: K562_WT_AS; Homo sapiens; OTHER
Replication timing: To get the pure G1 phase cells, 1 × 108 K562 cells were washed twice with cold PBS and fractionated by elutriation at each of the following flow speeds: 15 × 2, 16 × 2, 17 × 2, 18 × 2 19 × 2, 20 × 2 ml/min. Each fraction was stained by DAPI in PBS and confirmed by FACS. Genomic DNA from G1 phase and asynchronized K562 cells was extracted simultaneously according to manufacturer's instructions (Qiagen, 69506). TruSeq_Pair_End_Seq_Sample_Prep