Pooled whole body transcriptomic sequencing of early (mostly 1st instar) H. limbata nymphs
Illumina compatible library was constructed using an Illumina TruSeq RNA Prep Kit V2 with index Set A (RS-122-2101), following the low sample input protocol (Part #15026495 Rev. F). 1 g of total mRNA was used as initial input; mRNA was then purified, fragmented, and primed with random hexameres using poly-T oligo attached magnetic beads. cDNA was reverse-transcribed with SuperScript II Reverse Transcriptase, 3 adenylated, ligated with RNA adapter indices, and PCR-enriched. cDNA library was checked for quality with an Agilent 2100 Bioanalyzer, and normalized to 10 nM.