RNA-Seq of Dalbergia melanoxylon: pooled sample DBME_gp1_seq1
Pooled RNA samples were sent to the Oxford Genomics Centre (Oxford, United Kingdom) for library preparation and sequencing. The Poly(A) mRNA fraction was isolated from the total RNA with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), and then pooled libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs). The prepared libraries were size selected, multiplexed and quality-checked, and finally sequenced on the HiSeq4000 (Illumina) with the paired-end read length of 150 bp. The raw reads were obtained in fastq files after in-house preliminary quality check.