GSM4647686: pGIPZ-Ethl-1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using the nucleospin RNA kit (Takara) according to the manufacturer's recommended protocol. RNA-seq libraries were prepared from 0.5 μg high-quality DNA-free total RNA by using NEBNext® Ultra RNA Library Prep Kit, according to the manufacturer's instructions. The libraries passing quality control (equal size distribution between 250-400 bp, no adapter contamination peaks, no degradation peaks) were quantified using the Library Quantification Kit from Illiumina (Kapa Biosystems, KK4603).using the Illumina provided protocol. RNA-Seq samples were sequenced on an Illumina HiSEQ4000 using Illumina provided reagents and protocols