WGS of Neisseria gonorrhoeae
Genomic DNA was extracted from pure isolates using the JANUS automated workstation with the Chemagic Viral DNA/RNA kit (PerkinElmer). Dual-indexed pair-end libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina). Libraries were sequenced on the Illumina NextSeq 500 (2 x 150 cycles) per Illumina protocols.