GSM3042013: Mutant_LowExpression_Rep1; Mus musculus; RNA-Seq
RNA from Six2-expressing progenitors isolated by FACS was extracted using TRIzol. 10 ng of total RNA were subjected to cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing. 5ng of cDNA were then used in a NEBNext DNA Library Prep kit (E6040S) to generate sequencing libraries according to the manufacturer's instructions. 5ng of cDNA was used in a NEBNext DNA Library Prep kit (E6040S) to generate sequencing libraries according to the manufacturer's instructions.