GSM2011072: DC90_0h; Oryza sativa; RNA-Seq
Whole plants of each sample (three replicates for each) were harvested for each genotype before and 36h time point of chilling treatment, respectively. Total RNA of each sample was isolated by TRIZOL. Equal amount (3 µg) total RNA isolated from three replicates of each sample were mixed to get four group pooled samples. A total amount of 3 µg RNA of each pooled group was used as input material for generating sequencing libraries using NEB Next® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s instruction.