EA_G2 HiSeq Illumina
Library preparation: - Fragmentation of DNA - Adapter ligation - Gel fractionating - Pooling of tagged libraries to one pool   Analyses of one pool on a HiSeq 2000: - up to 180.000.000 clusters in sum =180.000.000 read pairs); - read length 100 bases; raw data output of up to 36 Gb in sum   - FastQ Files (sequences and quality scores)  Use of 5 μg of high molecular, RNA-free DNA (concentration approx. 200 ng / μl; preferably dissolved in RNase-, DNase- and protease-free molecular grade water) per sample.