GSM1645528: Control Replicate 2; Mus musculus; RNA-Seq
RNA was isolated from laser-captured cells using PicoPure RNA isolation kit (Applied Biosystems).Total RNA isolated by LCM (2.3 ng to 33.3 ng per sample) was amplified using the Ovation RNA-Seq System V2 (NuGEN). 300 ng of each sonicated cDNA sample was used for RNA-Seq library preparation. cDNA sequencing libraries were completed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), Illumina multiplexing adaptors, and the NEBNext ChIP-Seq DNA Sample Prep Reagent Set 1 Kit (New England Biolabs). Single-read sequencing was performed on the Illumina HiSeq 2000 (100 bp reads).