30 - 16S amplicon
Water samples were collected, and filtered through glass fiber filter using vacuum pump. DNA were extracted from the filters, and PCR amplification of 16S rRNA gene fragments was performed using CS1_341F and CS2_806R primers. PCR amplicon was sequenced using Illumina NextSeq 500. Raw reads were sorted by barcodes. Barcode and primer sequences were trimmed and data was analyzed using qiime (version 1.91) and mothur combined pipeline to filter out low quality sequences with specified parameters.