GSM2521713: S2 T4 H3 ChIP rep1 6.25U MNase; Drosophila melanogaster; MNase-Seq
[MNase-seq] MNase titration was performed as described previously (Mieczkowski, Cook, Bowman et al. Nat. Communications 2016). For h-MACC, after addition of EDTA/EGTA and SDS half of the digests were kept at 4C as input, the other half were adjusted to ChIP buffer conditions [10mM TRIS ph8.0, 100mM NaCl, 1mM EDTA, 0.1% sodiumdeoxycholate, 0.5% sarkosyl, 1% triton x-100 and complete protease inhibitors (Roche)] with 1ml of ChIP buffer. After tumbling for 10min at 4C, the digests were spun at high speed for 10min at 4C and the supernatant was incubated with anti H3 antibodies (abcam ab1791) as described (Bowman et al. BMC Genomics 2013).