NB89
In order to formulate hypotheses about the function of hypothetical gene functions in the strict anaerobe, Methanosarcina acetivorans, we developed semi-throughput anaerobic techniques to UV mutagenize, screen, and select for mutant strains in 96-well plates. From the mutant strains, genomic DNA from 1 ml stationary-phase culture was extracted using the Qiagen Blood genomic DNA extraction kit. Libraries were prepped with the Nextera XT DNA Library Prep Kit.