guide RNA transcriptome, procyclic stage Eatro 164 T. brucei brucei
T. brucei brucei clone IsTar from stock EATRO 164.  Mitochondrial vesicles were collected using a series of differential spins and RNA isolated.  The gRNA sized RNAs were then size-fractionated by denaturing 10% PAGE.  The Illumina "small RNA" sample preparation protocol was followed for library construction.