NextSeq 500 paired end sequencing; Comparison of seven single cell Whole Genome Amplification commercial kits using targeted sequencing
Comparison of seven single cell Whole Genome Amplification commercial kits using targeted sequencing
No collection. Commercial Hela genomic DNA (NEB) The following scWGA kits were used (Kit short in manuscript, Kit name, Cat. Number, WGA technique, Manufacturer): <br> Ampli1, Ampli1 WGA Kit, WG 001 050 R02, Linker adapter PCR (LA-PCR), Silicon Biosystems<br>MALBAC, MALBAC Single Cell WGA Kit, YK001B, Multiple Annealing and Looping Based Amplification Cycles (MALBAC), Yikon Genomics<br>GenomePlex, GenomePlex Single Cell Whole Genome Amplification Kit (WGA4), WGA4, Degenerate oligonucleotide-primed PCR (DOP-PCR), Sigma<br>PicoPlex, PicoPLEX WGA Kit, E2620S, displacement DOP-PCR (D-DOP-PCR), New England Biolabs (manufactured by Rubicon)<br>GPHI-SC, illustra Single Cell GenomiPhi DNA Amplification Kit, 29-1081-07, Multiple Displacement amplification (MDA), GE Healthcare<br>RepliG-SC, REPLI-g Single Cell Kit, 150345, MDA, Qiagen<br>TruePrime, TruePrime Single Cell WGA kit, 350025, MDA, SYGNIS Targeted enrichment using Access Array (Fluidigm) using the protocol as described in (Biezuner et al. Genome Research. 2016.) Sequencing primers are described in manuscript Supplementary Table 1.