GSM2300515: CNT-exposed Bl/6 _3; Mus musculus; RNA-Seq
Animals were sacrificed via pentobarbital/thiopental and exsanguinated.  Lungs were perfused with 10mL PBS via right ventricle.  Right lung lobes were lavaged with PBS, excised, snap-frozen in liquid nitrogen, and stored at -80deg C.  RNA was isolated from lungs using TRI reagent (http://mrcgene.com/) and purified using adsorption columns (miRNeasy, Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols