Year-2_roots_DNA_Salvia.sclarea_inoc_replicate = biological_replicate_3
500 mg of ground root samples were used for total genomic DNA extraction using an adapted method, using Cetyltrimethylammonium bromide, Polyvinylpyrrolidone and activated charcoal extraction. The V3-V4 region of the 16S rDNA was then amplified in a PCR reaction using the 341F/805R primer pair, containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.