GSM4647773: NZM6 (RRBS); Homo sapiens; Bisulfite-Seq
Genomic DNA cell lines was extracted using a QiAamp DNA mini kit (QIAGEN, Catalog # 51104). The  extracted genomic DNA was digested with MspI. This step was followed by end-repair, A-tailing and adapter ligation. Adapter ligated MspI fragments were size selected, by gel-based size selection method, after 15-20 cycles following which they were bisulfite converted and amplified by PCR. The amplified PCR product was purified and quantified by Qubit dsDNA HS kit (Invitrogen Catalog #Q32854). An Agilent Bioanalyzer assay was used to determine the quality of each library and the size distribution of the DNA fragments within each library. Single-ended RRBS libraries with 100 bp read length were sequenced on an Illumina HiSeq2500 with high output run mode and SBS v4 chemistry.