GSM2298930: CCF3038xp2.CD133POS; Homo sapiens; RNA-Seq
Cells were dissociated from the tumor, depleted of mouse cells twice by Miltenyi mouse depletion kit and MACS magnetic sorting and then lysed in Trizol. RNA was extracted with TRIzol (Invitrogen), separated using Phase Lock Gel tubes (5 Prime), and purified using the miRNAeasy kit (Qiagen) according to the manufacturer’s protocol. Total RNA was prepared for sequencing by Beckman-Coulter Genomics using the Illumina TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's protocol.