GSM4647489: Tissue associated B cells; Mus musculus; RNA-Seq
Heart, blood, lung, and liver were isolated from the same mice. B cells were isolated by flow sorting using a FACS Aria-II Cell Sorter. Doublets and dead cells were excluded. Flow-sorted B cells from each tissue/organ were all labelled with Hashtag-C antibodies. Blood was labelled with Hashtag-C #1, lung with Hashtag-C #2, liver with Hashtag-C #3, heart with Hashtag-C #4. Blood, heart, lung and liver were submitted to the same digestion protocol and B cells were sorted on the same day. 10x Genomcis Chromium single cell preparation