WAX Replicate 1
Ribosomal RNA was removed from 1 microgram of total RNA using the Ribo-Zero rRNA removal Meta-bacteria kit (Epicentre Biotechnologies, Madison, WI). The mRNA-enriched fraction was converted to indexed RNAseq libraries with the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA), and the final libraries were pooled in equimolar concentrations and quantitated by qPCR (Library Quantification kit, Kapa Biosystems, Woburn, MA).