GSM2791535: AB6_DENV24h_2; Mus musculus; RNA-Seq
Total RNA was extracted from spleen tissue using an RNeasy minikit. Polyadenylated RNA was isolated using the Poly(A)Purist MAG kit and was subsequently fragmented using RNAse III. 25 ng of each sample was used in the SOLiD Total RNA-Seq Kit, per the manufacturer’s instructions, to generate barcoded, strand-specific SOLiD libraries. The libraries were then converted into SOLiD Wildfire compatible fragments using the 5500 WF Conversion Primer Kit and protocol supplied by Life Technologies. Sequencing was performed with a SOLID 5500WF sequencer, producing between 36 to 190 million single-end reads with 35 nucleotide lengths.