GSM3041129: H3K9me2 6299; Schizosaccharomyces pombe; ChIP-Seq
Lysates were generated by bead beating and chromatin was sheared by sonication. Cleared lysate was incubated with antibody for 2 hours at 4C and immunoprecipitated genomic DNA was purified. Libraries were prepared for Illumna sequencing using manufacturer's protocols, starting with ~5ng immune-precipitated DNA fragments. Each library was generated with custom-made adapters carrying unique barcode sequence at the ligating end. Barcoded libraries were mixed.