RNAseq of WT mus musculus colon
RNA-seq and library preparation were performed at the University of Wisconsin Biotechnology Center DNA Sequencing Facility. Poly-A selection was employed to enrich high quality mRNAs from total RNA samples (1 ug input per sample) and library preparation was accomplished using the Illumina Truseq Stranded mRNA preparation kit (Illumina Inc.). After PCR enrichment of DNA fragments with adapter molecules, an Agilent DNA 1000 Chip (Agilent Technologies) assay was performed to verify the library profile and size