GSM3777053: Sm2B3001: control; Sinorhizobium meliloti; ChIP-Seq
Lysates were clarified and F-DnaA-DNA complexes were isolated with magnetic beads coated with Flag antibody. Quality of the DNA samples were checked with the Agilent High Sensitivity DNA Kit using the Agilent 2100 Bioanalyzer (Agilent) before sequencing using MiSeq (Illumina) in accordance with the manufacturer's instructions. The barcoded ChIP-Seq libraries yielded approximately 800 000 reads per sample of 75 bp length. Sequence reads were mapped onto the reference genome (S. meliloti 1021; GCF_000006965.1_ASM696v1)