Input for H1 hESCs ChIP-seq
H1 hESCs were grown on Matrigel in mTESR for 5 days, cross-linked with 1% formaldehyde for 15 mins and harvested. Chromatin was isolated as described (Oler et al., NSMB 2010) and input used for IPs was purified to use as a control. Library preparation was carried out per Illumina's guidelines (using the TruSeq library prep kit).