MCF-7, RNA-Seq, cDNA synthesis performed on microarray surface, Replicate 2
The forward read (R1) is a 31 nt sequence where the first 18 bp is a barcode (cell ID). This is followed by 9 nt of a semi-randomized UMI sequence used to distinguish duplicated transcripts. The reverse read (R2) is 121 nt used to map and annotate transcripts. The insert size between R1 and R2 is approximately 250 nt.