RNA-Seq of mouse: Lys(+) GPX(+/-) FvB AOM, male, 12592
Total RNA was extracted from fresh frozen mouse tissues using the RNeasy Micro Kit (Qiagen) according to the manufacturer큦 recommendations. RNA-Seq library preparation was performed from 800ng of the extracted total RNA using TruSeq Stranded mRNA LT-Set A (Illumina) and 12 cycles of PCR amplification. The libraries were diluted to 2 nM or 10 nM, according to their final concentration, and clustered at 10 pM using the TruSeq PE Cluster Kit v3 - cBot - HS or the HiSeq Rapid PE Cluster Kit v2. The RNA libraries were sequenced as an 8-plex in one lane. All NGS libraries were sequenced paired-end 2 x 50 bp on an Illumina HiSeq2500 platform using two TruSeq SBS Kit v3 - HS (50 cycles) or HiSeq Rapid SBS Kit v2 (50 cycles) kits (Illumina).