GSM2610644: Cdx2-/- Villus KDM6A ChIP-seq biological rep1; Mus musculus; ChIP-Seq
Whole cell extracts from villi fixed with 1% formaldehyde were sonicated and incubated overnight with Dyna beads (Thermo Fisher Scientific) conjugated with 5 ug KDM6A antibody (Cell Signaling Technology, 33510) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.