GSM3963713: Root apical meristems, GFP_INPUT_rep2; Arabidopsis thaliana; ChIP-Seq
Nuclei were extracted after formaldehyde-fixation of root meristem tissues. Nuclei were lysated and their content sonicated. Protein-DNA complexes were immuno-precipitated with anti-GFP antibody from sonicated material. Libraries were prepared according to NuGEN's instructions accompanying the Ovation® Ultralow V2 DNA-Seq Library Preparation Kit (Part# 0344NB). Libraries were sequenced on the Illumina HiSeq2000 following the manufacturer's protocols.