A field study of heavy oil-produced water treatment by regulating indigenous microorganisms in a full-scale plant
For determination of the microorganism structure of HOPW treatment process, water samples in each procedure were taken from the treatment system.The water samples mentioned above were concentrated by vacuum filtration. The filters were cut into pieces and total DNA of each sample was extracted. For Illumina MiSeq sequencing, the V3-V4 region of 16S rRNA of bacteria and archaea in the eight DNA samples were amplified.