454 seq of Oomycetes ITS : Trasimeno 09c
The first PCR was with primers ITS6 and ITS4 (194 °C for 5 min, 15 cycles of 94 °C for 30 s, 55 °C for 30 s, 72 °C for 1 min, and 72 °C for 10 min). A second PCR used ITS6 and ITS7 (194 °C for 5 min, 25 cycles of 94 °C for 30 s, 59 °C for 30 s, 72 °C for 1 min, and a final 72 °C for 10 min)