Low_Carbon_Stage1_Late_Dark_replicate_E
Total RNA was extracted using the Spectrum Plant Total RNA kit (Sigma Aldrich-STRN50). Genomic DNA contamination was removed with on-column DNase digestion (Sigma Aldrich-DNase 10). A total of 56 barcoded transcriptome libraries were prepared using the Illumina Truseq Stranded mRNA HT library prep kit (cat# RS-122-2103). Libraries were pooled, denatured and diluted according to the NextSeq 500 protocol. Paired-end sequencing of libraries was performed on the Illumina Nextseq 500 platform using a high-output 300 cycle v2 flowcell