GSM3403738: H3K27me3-TestisPortion-P7-Wt-To-R2-L6093; Talpa occidentalis; ChIP-Seq
Chromatin immunoprecipitations were performed using the iDeal ChIP-seq kit for Histones (Cat. No. C01010051) according guidelines. Whole fixed gonads were sonicated with a Bioruptor (45 cycles 30sec on, 30 sec off at high power) in the shearing buffer from the kit. 5 mg of chromatin was used in each immunoprecipitation with 1mg of the following specific histones antibodies: anti-H3K4me3, Millipore, cat. No. 07-473; anti-H3K4me1, Diagenode, cat. No. C15410037; anti-H3K27ac, Diagenode, cat. No. C15410174; anti-H3K27me3, Millipore, cat. No. 07-449. Libraries were prepared using the Nextera adaptors and sequenced