Illumina HiSeq 2500 paired end sequencing; RNA-seq of human skin lesions diagnosed as actinic keratosis, intraepidermal carcinoma or squamous cell carcinoma
RNA-seq of human skin lesions diagnosed as actinic keratosis, intraepidermal carcinoma or squamous cell carcinoma
Skin lesions samples were collected from consenting patients that presented to the Dermatology department in Princess Alexandra Hospital from 2011-2013 Total RNA was extracted using TRIzol (Life Technologies, ThermoFisher Scientific, Victoria, Australia) and RNA purification was performed using the RNeasy mini kit (Qiagen, Victoria, Australia). RNA was assessed for quality and quantity using a Nanodrop instrument.  RNA sequencing libraries of poly (A) RNA from 500ng total RNA obtained from AK, IEC and SCC skin lesions were generated using the TruSeq unstranded mRNA library prep kit for Illumina multiplexed sequencing (Illumina, San Diego, CA, USA).