GSM1985878: HeLa dataset B; Homo sapiens; OTHER
Whole cell RNA was extracted using Trizol reagent after which LSU and SSU rRNA was isolated from agarose gels by passive diffusion RiboMeth-seq: 5-10 μg of RNA was partially degraded by alkaline at denaturing temperatures. The size fraction 20-40 nt was purified on gels and linkers added using a system relying on a modified Arabidopsis tRNA ligase joining 2’,3’ cyclic phosphate and 5’- phosphate ends. The library fragments were then sequenced on the Ion Proton platform. See Birkedal U, Christensen-Dalsgaard M, Krogh N, Sabarinathan R, Gorodkin J, Nielsen H. Profiling of ribose methylations in RNA by high-throughput sequencing. Angewandte Chemie. 2015;54(2):451-5 for detailed description.