DNA-seq of Amazonian environmental samples from insects
Samples from Slam-trap opened 24h; DNA extracted using lysis buffer; PCR targeting V3-V4 region (~460 bases) of the 16S rRNA gene using the forward primer (5'-CCTACGGGNGGCWGCAG-3') and reverse primer (5'-GACTACHVGGGTATCTAATCC-3') of prokaryote; Macrogen companie amplification and Illumina MiSeq sequencing