Targeted long-read sequencing of homo sapients: 8 tissue mixture, cDNA enriched for 2kb+ lengths
cDNA was prepared using the SMARTer cDNA synthesis kit (Clontech). The cDNA was size selected using SPRI beads, so that cDNA molecules above ~2kb were enriched. The cDNA was subjected to hybridization-based enrichment, targeting cDNAs (isoforms) corresponding to 763 human transcription factors. The captured material was amplified by PCR and then converted into a SMRTbell library using the SMRTbell Template Prep Kit 1.0 (Pacific Biosciences).