GSM1632651: Sufficient iron condition 2; Paenibacillus riograndensis SBR5; RNA-Seq
Total RNA was extracted from bacterial cells using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was verified by electrophoresis on a 1% agarose gel. After the samples were treated with DNase I (Invitrogen), the concentration and purity were determined by spectrophotometer readings at 260 nm and 280 nm using a NanoDrop 2000 (Thermo Scientific). To remove ribosomal RNA, total RNA was processed using a MICROB Express Bacterial mRNA Enrichment Kit (Ambion from Life Technologies), precipitated with ethanol and resuspended in nuclease-free water. cDNA libraries were constructed using a Whole Transcriptome Analysis Kit (Life Technologies) followed by full-scale emulsion PCR according to the supplier’s instructions.