GSM3040062: K9me2_Col-0_rep1; Arabidopsis thaliana; ChIP-Seq
Three grams of 10-day seedlings were crosslinked with 1% formaldehyde. Lysates were clarified from sonicated protein-DNA complexes were isolated with selected antibodies. For histone modifications ChIP, Dynabeads Protein A or G Magnetic Beads (Thermo Fisher Scientific) with the anti-H3K9me2 (Abcam, cat #Ab1220); H3 (Millipore, cat # 04-745); anti-H3K27me1 (Millipore, cat #07-448) antibodies are used; Anti-FLAG M2 magnetic beads (Sigma-Aldrich, M8823) were used for FM-SE. ChIP-seq libraries were generated using the Ovation Ultralow DR Multiplex System (NuGEN), two to ten microgram of ChIPed DNA were used as the starting DNA and 18 cycles were used for the library amplification step.