P6 metabarcoding from soil in TLT11-15 library constucted using soft gridding strategy
DNA metabarcode library constructed a using trnL P6 loop DNA barcode (Taberlet et al, 2007) amplified from TLT11-15 soil, in the Salar the Atacama Basin, Antofagasta, Chile. For this library a soft gridding strategy was use to extract DNA from soil, where the soil was agitated one times in in FastPrep-24 Instrument (MP Biomedicals, EEUU), aiming to free DNA and soft plant remains . Details in Carrasco-Puga et al (unplublished)