GSM2254849: 9wk_133; Mus musculus; Bisulfite-Seq
DNA was extracted using a Qiagen all-prep DNA/RNA kit RRBS libraries were constructed using the Truseq DNA LT kit, with a bisulfite treatment after adapter ligation. For locus specific bisulfite sequencing, we bisulfite treated DNA and PCR amplified regions of interest using primers with a 5' tail. A second PCR recognizing the 5' tail added barcode and adapter sequences.