Transcription analysis of leaf under dark stress at seedling stage in maize
One maize inbred line Q319 considered as a stay green inbred during the growth stage, was grown in greenhouse for three replicates after germination in culture dish. The growth condition was 12h light and 12h dark for the photoperiod, 60% humility, 25℃ temperature and 400μmol·m-2·s-1 illumination intensity. When the seedlings grew into a stage with two leaves fully expanded (V2 stage), three plants with the over-ground part from the bottom of the first leaf were sampled for RNA extraction and another three plants for chlorophyll determination in each replication. Meanwhile, the rest plants were moved to dark environment with same humility, temperature and illumination intensity, as above. After dark treatment for four days, sample as the first time and move the rest plants back to the condition with same illumination intensity as the initial. Samples was named the sample before dark treatment as RNL, sample after dark treatment as SDT and sample after recover light as SRNL.