Small RNA-Seq from Paracoccidioides brasiliensis Pb18: yeast phase
Mycelial, transition and yeast total RNA was extracted and incubated with DNAse (RQ1 RNase-free DNase, Promega). Libraries were constructed with the NEBNext Multiplex Small RNA Library Prep Set for Illumina. Sequencing was performed using the Illumina HiSeq 2500/2000 platforms.