Amplicon sequencing of DENV-3 RNA from human PBMCs
The DENV-3 genome was amplified via reverse transcription and PCR using sequence-specific primer pairs to generate 12 overlapping amplicons per sample. Multiplexed libraries for high-throughput sequencing were constructed from pooled amplicon mixes and sequenced on an Illumina HiSeq 2000 platform to yield 150-nucleotide (nt) paired-end reads in forward (Ôread 1Õ) and reverse (Ôread 2Õ) orientations.