Illumina HiSeq 2500 sequencing; Expression profiling of the aging epidermis [RNA seq]
Expression profiling of the aging epidermis [RNA seq]
Suction blisters of 7 mm diameter were taken from the volar forearms of all subjects and immediately stored in liquid nitrogen. Samples were homogenized using an MM 301 bead mill (Retsch) and RNA subsequently extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen) according to manufacturer's instructions. Libraries were prepared using TruSeq Library Prep Kit (Illumina) following manufacturer's instructions.