GSM3039986: liver_smallRNA_miR122_floxCre_d14_repC; Mus musculus; ncRNA-Seq
Liver samples were snap frozen in liquid nitrogen and ground into powder.  Samples were homogenized and RNA was extracted by Trizol RNAseq library samples  were prepared using an Illumina TruSeq kit 3ug of RNA per sample was ligated to Linker-1 (IDT) in the absence of ATP with T4 RNA ligase 1 (New England Biolabs). Ligated RNA was size selected (based on an 18-36nt small RNA fraction) on 15% PAGE. The 5' ends RNA were ligated to 4nt barcoded adapters. Dual-ligated RNA was reverse transcribed, PCR amplified and size selected on 4% NuSieve (Lonza) agarose.  Small RNA sequencing was performed on the Illumina miSeq platform.