GSM1173200: DW replicate; Brachypodium distachyon; RNA-Seq
Total RNA was extracted from 12-day-old ABR5 seedlings using the mirVana miRNA Isolation Kit (Ambion, Austin, USA) following the manufacturer's protocol. PARE libraries were prepared as described previously (Nature Protocols 2009, 4:356-362.). Modification was made according to (Plant Cell 2010, 22:1090-1103). Sequencing was performed on Illumina GAIIx.