RNA-seq of T. aotearoense LA1002: as control
Start strain of LA1002 was cultured with 30 g/L mixed sugars (glucose:xylose = 2:1, g:g). When the cell density of OD600 reached 0.8¨C1.0 (in exponential phase), 1.0 OD cell was harvested via centrifugation (12000 rpm ĄÁ 2 min) at 4 ĄăC. Total RNA was extracted using RNAprep pure kit (for cell/bacteria, TIANGEN, Beijing, China) according to the manufacturerĄŻs instructions.