GSM2522535: K326_Shoot_ZT6_R1; Nicotiana tabacum; RNA-Seq
Total RNA was extracted using QIAzol followed by clean up, including on-column DNase treatment, using Qiagen RNeasy spin columns. RNA was QC'd using Bioanalyzer RNAnano protocol. RNA with RIN>=8.0 was used in library preparation Random primed cDNA-libraries were prepared at GATC Biotech (Konstanz, Germany) using propritary methods