GSM3775675: L7.2; Saccharomyces cerevisiae; OTHER
The ribosome was immunoprecipitated from lysate and then treated with RNase I. Total RNA was extracted by TRIzol reagent from the IPed ribosome. Ribosome profiling libraries were generated as described in MGlincy, N.J. and Ingolia, N.T., 2017, Methods, 126:112-129 .