Simultaneous RNA-seq of bone marrow derived dendritic cells from Mus Musculus C57BL/6 cocultured with Candida albicans strain SC5314 over a 2 hour infection time course
Simultaneous RNA-seq of bone marrow derived dendritic cells from Mus Musculus C57BL/6 cocultured with Candida albicans strain SC5314 over a 2 hour infection time course
Candida albicans was grown in 1% yeast extract, 2% peptone, and 2% glucose and collected in the logarithmic growth. Dendritic cells were derived from C57BL/6 femur bone marrow in DMEM supplemented with 10% FCS, and 15 % GM-CSF. Dendritic cell and Candida albicans coculture at a target ratio of 1:1 Samples were incubated at 37C 5% CO2, 95% humidity between 0 and 120 min. We isolated total RNA from samples using the SV total RNA isolation system (Promega). Cells were scraped in the provided lysis buffer followed by homogenization with 0.5 mm acid washed glass beads. Extraction was performed following manufactures protocols.