WGS of  Canis familiaris: B-cell lymphoma (G1-BuffyCoat)
Samples  from non-neoplastic tissue (blood mononuclear cells) and lymphoma (fine  needle aspirate samples were collected from either the prescapular,  submandibular, or popliteal lymph node) were collected at the time of  diagnosis. DNA was isolated using the DNeasy Blood and Tissue kit (Qiagen,  Valencia, CA)  following the manufacturers protocol. Sample quantity and purity were  assessed with a NanoDrop spectrophotometer (260/280 > 1.46, 260/230 >  0.45) and Qubit fluorometric quantitation. Samples were sent to McGill  University and Gnome Qubec Innovation Centre, Montral, QC, Canada, for  Illumina HiSeq X Ten PE150 sequencing with qPCR and using  Illumina TruSeq DNA v3 adaptors.