GSM4696319: ChIPseq_CTCF_ZT0_rep2; Mus musculus; ChIP-Seq
For ChIP-seq, liver tissue for two biological replicates at ZT0 and ZT12 was dissected as processed as for Hi-C and then fixed in 1% formaldehyde for 5 minutes. Chromatin immunoprecipitation was performed as described (Rubin et al., 2017) using 10 _g of _-CTCF (Millipore, 07-729). DNA was purified using Zymo Research DNA purification columns.  Sequencing libraries were prepared with the NEBNext ChIP-seq library prep kit (NEB) according to manufacturer's instructions. DNA was purified using AMPure beads (Agencourt). NEBnext library preparation protocol