GSM2610683: Cdx2-/- Villus RNA-seq biological rep2; Mus musculus; RNA-Seq
Villi were collected in TRIzol reagent (Thermo Fisher Scientific) and RNA was isolated using RNeasy kit (Qiagen) with on-column Dnase-treatment (Qiagen). The TruSeq RNA Sample Preparation Kit V2 (Illumina) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.