Illumina HiSeq 2000 paired end sequencing
Illumina Nextera MP kit
Mate paired libraries - 6-13kb. The DNA fragments were circularized then sheared to a 300 bp insert size using the Covaris E220. Following end repair and adapter ligation the samples was enriched using 15 cycles of PCR for the Pippin Prep library and 10 cycles for the gel free library