GSM2363456: foxj1_crispr_RNAseq_9h_rep_1; Xenopus laevis; RNA-Seq
mRNAs were obtained with proteinase K digestion, phenol chloroform extraction and precipitated with ethanol, treated with DNase, extracted with phenol chloforom, and precipitated with lithium and again with ethanol. See our manuscript (PMID: 24934224) for a more detailed protocol.  RNAseq libraries were constructed with Illumina Truseq v2 non-stranded mRNA kits. Chromatin was obtained as in Ma et al. 2014 (PMID: 24934224) Illumina Truseq v2, poly-a+