Illumina metagenome of Mushroom Spring: 65C
Mat samples were collected using a #4 cork borer on 2003-10-02, immediately frozen on dry ice in the field, and kept frozen at -80C until analysis. DNA was extracted and purified with the fastDNA spin kit (Molecular Biosciences) according to the manufacturer's instructions. Cells were suspended in the manufacturer's lysis solution and lysed by bead beating in a Fastprep cell disrupter (Bio101 Savant Instruments) for 45s at a speed of 6.5 m/s.