GSM2703883: Rroid KO_B3_RNA-seq; Mus musculus; RNA-Seq
The aqueous phase from the TRIzol-chloroform extraction was precipitated with ethanol, applied to RNeasy Micro columns and processed according to the manufacturer’s instructions The RNA-Seq libraries were generated using the Ovation RNA-Seq System V2 (Nugen) and the KAPA Hyper Prep Kit (KAPA Biosystems). 1x100bp sequencing was performed on the HiSeq2500 using the TruSeq Rapid SBS Kit