title: pMX AIDmm L1
design: Virus integration libraries were made using modified protocol for generating TC-Seq libraries described in (Oliveira et al., 2012). Briefly, genomic DNA was extracted with phenol/chloroform and then fragmented by sonication at low power for 7 cycles (30′′ on/30′′ off) in a Bioruptor (Diagenode) to yield a 500–1350 bp distribution of DNA fragments. DNA was blunted by End-It DNA Repair Kit (Epicentre) and A-tailed. DNA fragments were then ligated to linkers. Pooled linker-ligated DNA was amplified by semi-nested ligation-mediated PCR using biotinylated primer.