title: Emergence of Clostridium novyi infection and mortality in a free ranging wild Asian elephant population, Southern India
design: DNA was extracted from Liver tissue of Elephas maximus indicus using DNA Isolation kit (MO BIO Laboratories, Carlsbad, CA). We designed and synthesized primers needed for amplification of 16S V3-V4 region and build a library and sequenced on Illlumina HiSeq 2500 platform. The samples were then used as template for PCR amplification. Libraries from the sample was tracked using a sequence barcode that was incorporated in the libraries generated. Bioinformatics QIIME pipeline was used to identify the presence of Clostridium novyi species.