title: GSM1470353: control-CM, experiment1; Homo sapiens; miRNA-Seq
library_construction_protocol: 18 h post ET-1 stimulation, cells were harvested with Total RNA Purification 96-Well Kit (Norgen Biotek Corp.).  Total RNA was extracted per manufacturer’s recommendations, resuspended in nuclease-free water, and quantified by UV spectrophotometry (NanoDrop™ 2000, Thermo Scientific). MicroRNA libraries from rRNA depleted total RNA (Ribo-Zero rRNA removal Kit, Epicentre) were prepared using Ion Torrent Total RNA Seq small RNA kit as per manufacturer’s recommendations (Life Technologies).