title: RNA-seq of Zea mays: 14 day endosperm
design: Three maize inbred lines, B73, W22, and PH207, were grown in the field in Saint Paul, MN in the summer of 2018. Reciprocal crosses between each pair of genotypes were performed. Ears were collected 14 days after pollination and endosperm was isolated using manual dissection, with approximately 10 kernels per ear pooled for each biological replicate. Paired-end, stranded RNA-seq libraries were created using Illumina TruSeq and sequencing was performed with the Illumina HiSeq at the University of Minnesota Genomics Center. On average, > 45 million reads were generated per library