title: WES of homo sapiens: melanoma: Sample Pt19-baseline
design: The gDNA and RNA were simultaneously extracted using mirVana miRNA isolation kit. The gDNA was subsequently fragmented on Covaris M220 sonicator. Illumina DNA library was prepared with Kapa LTP Library Preparation Kit for Illumina platforms. Exome enrichment was performed using Roche NimbleGen SeqCap EZ Human Exome Library v3.0 system. Sequencing was performed on Illumina HiSeq 2000 for pair end 100 run. Run quality check was done on Illumina SAV. Demultiplexing was performed with Illumina bcl2fastq2 software.