title: GSM2027389: 2_1c; Mus musculus; RNA-Seq
library_construction_protocol: For RNA extraction from LCM dissected motor neurons, the Arcturus PicoPure RNA Isolation Kit was used. For other samples, cells are diectly picked after dissociation and deposited into lyssis buffer. No RNA extraction is conducted.The cell lysis is proceed directly to library preparation. Libraries for RNA-seq were prepared by using SMART-seq2 combined with Tn5 tagementation