title: GSM2257349: Alzheimer's-affected human brain; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA isolated using modified guanidine thiocyanate technique from healthy human and Alzheimer's disease brain was procured from BioChain, USA. The purity of isolated RNA was examined using NanoDrop spectrophotometer (Thermo Scientific) by measuring absorbance at 260 nm and 280 nm. An aliquot of each the samples was also run on an Agilent RNA Bioanalyzer to check the RNA integrity number (RIN) and was found to be 8.1 and 8.5 in HB and AD sample respectively. The RNA libraries were constructed for sequencing following Illumina TruSeq Small RNA library protocol outlined in “TruSeq Small RNA Sample Preparation Guide” (Part # 15004197; Rev. F; February 2014) and using TruSeq Small RNA sample preparation kit (Illumina, # RS-200-0012).