title: GSM3045696: E2F-1 siRNA E2F-1 RIP-seq; Homo sapiens; RIP-Seq
library_construction_protocol: Cells are lysed in RIP-lysis buffer and E2F-1 RIP is performed.  RNA is extracted using Trizol, DNAse treated, and ribodepleted using a GeneRead rDNA Depletion kit Libraries were prepared using NEBNext Ultra Directional RNA library prep kit for Illumina