title: MiSeq of ectomycorrhizal fungi: roots of Abies and Betula saplings
design: To identify the EM fungi present on A. balsamea and B. papyrifera sapling roots, the ITS1 rDNA subunit was PCR amplified using a barcoded fungal-specific ITS1F-ITS2 primer set and cycling conditions detailed in Smith and Peay (2014). Amplified products were magnetically cleaned using the Agencourt AMPure XP kit (Beckman Coulter, Brea, CA, USA) and quantified using a Qubit dsDNA HS Fluorometer (Life Technologies, Carlsbad, CA, USA). Each of the A. balsamea and B. papyrifera root samples were pooled into a single library and sequenced at the University of Minnesota Genomics Center using the 250 bp paired-end MiSeq Illumina platform.