title: GSM4384797: RTF2-Rescued_24h IFN treatment_40h harvest_rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer's protocol. The Smart-Seq2 protocol (Picelli, Nat Protoc, 2014) was employed to perform transcriptomic analyses of the different cells. The cDNA was synthesized from 1ng of total RNA using SuperScript II reverse transcription, followed by PCR amplification and quality check using high-sensitivity DNA Bioanalyzer chip (Agilent). 0.15 ng of cDNA was then used for the tagmentation reaction carried out with the Nextera XT DNA sample preparation kit (Illumina) and further PCR amplification.