title: GSM3891016: WAT CD31+ endothelial cells Replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Cells were flow-cytometry-sorted into RPMI-1640, 10% fetal calf serum (FCS) plus penicillin/streptomycin, 2-mercaptoethano and transferred to Trizol (Life Technologies). RNA was extracted using RNeasy kits and reagents (Qiagen), DNA was digested using Turbo DNase (Ambion). RNA was concentrated using an RNeasy Micro Kit (Qiagen) and assessed using a Bioanalyzer (Agilent) RNA was processed for RNA sequencing using Ovation RNA-seq System V2 (Nugen), fragmented using the Covaris M220 and barcoded using Ovation Ultralow Library Systems (Nugen)