title: Illumina Genome Analyzer IIx paired end sequencing; An mRNA-sequencing atlas of mouse CD4+ T cell transcriptomes
design: An mRNA-sequencing atlas of mouse CD4+ T cell transcriptomes
library_construction_protocol: MACS was used to isolate splenic CD4+ T cells by depleting CD8a, CD11b, CD11c, CD19 Ly6G(Gr-1) expressing cells followed by positive selection for CD25+ cells. Poly-(A)+ RNA was purified from ~500,000 cells using the Oligotex kit (Qiagen). The manufacturers protocol was modified slightly to include additional final elution steps resulting in a larger volume. RNA was purified, reverse transcribed and prepared for sequencing described in http://msb.embopress.org/content/7/1/497