title: GSM4586575: hypoxia_KD_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen) and RNA concentration was measured using a Nanodrop spectrophotometer (ThermoFisher Scientific). Three replicates of each sample at 2 µg of RNA were submitted for sequencing and analysis (Technology Center for Genomics and Bioinformatics, UCLA). RNA sequencing and library preparation was using the KAPA RNA HyperPrep Kit with RiboErase (Roche Sequencing, cat#KK8561, Pleasanton, CA, USA), according to the manufacturer's instructions. The work flow consisted of rRNA depletion, cDNA generation, end repair to generate blunt ends, A-tailing, adaptor ligation, and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on the Illumina HiSeq3000 System for a single-read 50 run (Illumina, San Diego, CA). Data quality check was done on Illumina SAV. Demultiplexing was performed with the Illumina Bcl2fastq2 v 2.17 program.