title: GSM1835699: 77-6630449-plus ATc; Saccharomyces cerevisiae; OTHER
library_construction_protocol: After competitive growth of yeast strains and sample collection, yeast plasmids were purified using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Purified plasmids were used as a template for PCR with barcoded up and down sequencing primers that produce a double index to uniquely identify each sample.  PCR products were confirmed by agarose gel electrophoresis. After PCR, samples were combined and bead cleaned with Thermo ScientificTM Sera-Mag Speed Beads Carboxylate-Modified particles.  Sequencing was performed using Illumina MiSeq.