title: RNA-Seq of cell line NCI-PBCF-HTB133 grown on a polyacrylamide gel of a stiffness of 500 Pa and covalently laminated with fibronectin
design: RNA was extracted from pelleted cells by by Stanford Functional Genomics Facility using Qiagen All Prep DNA/RNA kit.  The extracted mRNA was then sequenced by Frederick National Laboratory for Cancer Research (FNLCR).  mRNA-seq libraries were pooled and sequenced on two sequencing flowcells (16 lanes total) of the HiSeq2500 with Illumina v4 chemistry, using paired reads with an insert size of 125. bp. The samples from the study have yields from 77 to 184 million reads. About 91-94% of pass filter reads have Q30 or above.