title: GSM2187857: ChIPSeq_STAT5_Mut_L1; Mus musculus; ChIP-Seq
library_construction_protocol: Frozen-stored mammary tissues from virgin or p13 were broken into powder with mortar and pestle. Nuclei were extracted in Farnham lysis buffer (5mM PIPES pH8.0, 85mM KCl, 0.5% NP-40 and protease inhibitors). Antibodies against STAT5A (Santa Cruz, sc-1081), H3K27ac (Abcam, Ab4729), GR (Thermo Scientific, PA1-511A) were used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.