title: GSM3204764: WT_rep1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: L1s were lysed in XB buffer from ARCTURUS® PicoPure® RNA Isolation Kit (Thermo Fisher), incubated 30 min at 42°C and snap-frozen in liquid nitrogen. After freeze cracking of the samples by 6 subsequent transfers from liquid nitrogen to 42°C, samples were let vigorously shake 10 min at RT and 10 min at 42°C. Next, RNA was extracted following the manufacturer's instructions, including an on column DNAse digestion NGS libraries were prepared from 2 ng of input RNA, using the Total RNA-Seq NuGen Ovation v2 kit in combination with the Illumina TruSeq DNA Nano kit, according to manufacturer's guides