title: GSM3204310: 16 hr TNF-a MLL4 ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP, cells were crosslinked, lysates from sonicated nuclei were prepared, and protein-DNA complexes were immunoprecipitated with corresponding antibodies. Please refer to the methods section for detailed protocols. ChIP-seq libraries were prepared according to Illumina's ChIP Truseq protocol. Briefly, ChIP DNA was end-repaired and adaptors were ligated to the ends of the DNA fragments. Adaptor-ligated ChIP DNA fragments with average size of 350 bp were used to construct libraries and single-end sequenced on Illumina HiSeq 4000.