title: GSM4460147: CAMA1_Cntrl_RNA-Seq_Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested and RNA was purified using a RNA purification kit (Maxwell, Promega). Samples were run on the Agilent Tapestation 4200 to ensure use of only high quality RNA (RIN Score ≥8). The Qubit fluorometer was used to determine sample concentration prior to preparation of libraries. One μg of total DNAse treated RNA was then prepared with the TruSeq Stranded mRNA Library Prep Kit from Illumina. Poly-A RNA was purified and fragmented before strand specific cDNA synthesis. cDNAs were then a-tailed and indexed adapters were ligated. After adapter ligation, samples were PCR amplified and purified with AmpureXP beads, then validated again on the Agilent Tapestation 4200.  Before being normalized and pooled, samples were quantified by Qubit then run on the Illumina NextSeq 500 using V2.5 reagents