title: GSM3890935: FC2E17; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell RNA-seq were conducted in mice on a C57BL/6J background before sexual maturation during embryonic and postnatal days E15.5-P23. Postnatal mice (P10, P23) were deeply anesthetized (5% isoflurane) and transcardially perfused with 40 ml ice-cold Cell were resuspended in PBS and concentrated up to 50-560 cells/μl. 33 μl of the solution form above together with 1 μl of lymphocyte suspension as spike-ins  were added to the Reverse transcription mix. All following procedures: cDNA synthesis, librald