title: GSM3890715: RIP_RNA_biorep1; Homo sapiens; RIP-Seq
library_construction_protocol: RNA from IPs was radiolabeled and run on a 10% TBE-Urea gel. RNA fragments from ~25-75 nucleotides were excised and extracted from the gel. cDNA libraries were prepared using the NEBNext Multiplexing Small RNA Library Prep Set for Illumina with 14-17 rounds of amplification, following the manufacturer's protocol with the exception that the 5' RNA adapter was replaced with a custom 5' adapter compatible with UMI (Unique Molecular Identifier) tagging (Kivioja et al., 2011) Libraries were sequenced using Illumina HiSeq 2500.