title: GSM4760965: G. lacustris, 12 degrees C, sample 3; Gammarus lacustris; RNA-Seq
library_construction_protocol: Frozen coarse powder of amphipods was homogenized in 1 ml of Qiazol Reagent (Qiagen) using an MM400 mixer mill (Retsch). Total RNA of amphipods was manually isolated using an RNeasy Mini kit (Qiagen). All samples were treated with DNase (TURBO DNA-free Kit). mRNA purification from total RNA was performed using an Oligotex mRNA Mini Kit. RNA concentration and quality were evaluated using Qubit 2.0 fluorometer and Agilent Bioanalyzer 2100. Twenty-four RNA-seq libraries were prepared from 20 ng of mRNA using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, SSV21124).