library_construction_protocol: "DNA was extracted using Genelute DNA extraction kit (Sigma).16S rRNA genes were amplified by PCR with primers 27FYM and 338R, incorporating unique barcodes and sequencing adapters. Amplicons were purified, quantified and pooled in equimolar proportions. Sequencing was performed using Illumina MiSeq platform and the V2 kit (250bp paired-end)"