title: GSM4698819: TyrRS rep1 input; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with formaldehyde (1% final concentration) for 10 min at room temperature. The reaction was stopped by adding 125 mM glycine. ChIP assays were performed according to the protocol of ChIP-IT Express Enzymatic kit (Active Motif) using ChIP-grade antibody against V5 (Thermo Fisher Scientific, R960CUS), IgG (Cell signaling technology, #5415), Libraries were prepared and sequenced by the Next Generation Sequencing Core at Scripps Research, La Jolla, California TF-ChIP-seq