title: GSM1184326: Nestin-peri, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from sorted Nes-peri and Nes-retic cells was extracted using the RNAqueous kit (Life Technologies, Grand Island, NY USA) and concentrated using RNA Clean and Concentrator columns (Zymo Research Corporation, Irvine, CA USA). cDNA was generated using a Clontech SMARTer Ultra Low RNA kit for Illumina Sequencing (Clontech Laboratories, Inc., Mountain View, CA USA) from 100pg-1ng of total RNA. cDNA was fragmented using Covaris (Covaris, Inc., Woburn, MA USA), profiled using an Agilent Bioanalyzer, and subjected to Illumina library preparation using NEBNext reagents (New England Biolabs, Ipswich, MA USA, catalog# E6040).