title: GSM1176679: Prdm4-EGFP_ChIP-seq_4; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were precleared with Protein G Dynabeads.  Complexes were then isolated with 6µg anti-GFP monoclonal antibody (Invitrogen A11120, clone 3E6, IgG2a) or control Mouse IgG (Santa Cruz sc-2025, Mouse IgG) linked to Protein G Dynabeads. Sequencing libraries were then prepared using the Illumina ChIP-seq DNA Sample Prep Kit.  Libraries were size selected at ~200bp and four libraries were multiplexed per lane on a HiSeq 2000.