title: GSM4585919: HG3, TP0; Homo sapiens; RNA-Seq
library_construction_protocol: Cryopreserved samples were thawed, pelleted and resuspended in cold PBS-0.04% BSA. 100,000 viable BFP+ cells were sorted into cold PBS-0.04% BSA, resuspended at 1x106 cells per mL, and barcoded with a 10X Chromium Controller (10X Genomics) by reverse transcription of RNA from individual cells. Sequencing libraries were subsequently constructed using reagents from a Chromium Single Cell 3' v2 reagent kit according to the manufacturer's instructions (10X Genomics, #PN-120267). Sequencing was performed on an Illumina NovaSeq platform (paired-end, Read 1: 26 cycles and Read 2: 98 cycles) according to the manufacturer's instructions (Illumina).