title: Illumina HiSeq 1500 sequencing; Elevated expression of the H3K36me2 demethylase Kdm8/Jmjd5, a direct Miz1 target gene causes prolonged postnatal Schwann cell proliferation in the murine Miz1∆POZ neuropathy
design: Elevated expression of the H3K36me2 demethylase Kdm8/Jmjd5, a direct Miz1 target gene causes prolonged postnatal Schwann cell proliferation in the murine Miz1∆POZ neuropathy
library_construction_protocol: Miz1 lox/lox mice were crossed with the desert hedgehog (Dhh)-Cre driver line (C57Bl6-Tg(Dhh- cre)1Mejr to achieve conditional ablation in Schwann cells of Miz1 exons 3 and 4, which encode the POZ domain (Sanz-Moreno et al. 2015). Mice had a mixed C57Bl6 and 129S2/SvHsd genetic background. Here, animals which were Dhh-Cre + ;Miz1 flox/flox are designated Miz∆POZ. Dissected sciatic nerve pairs of indicated ages were homogenized in TRI Reagent (Sigma, T9424). RNA was extracted, DNase treated (Macherey-Nagel, #740963), and purified using theNucleoSpin ® RNA Clean-up kit (Macherey-Nagel, #740948) according to the manufacturer's instructions. RNA from cell culture samples was extracted, DNase treated and isolated using theNucleoSpin® RNA kit (Macherey-Nagel, #740955). Sequencing libraries were prepared with the TruSeq Stranded mRNA Kit (Illumina).