title: GSM3197082: O(WT) replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: FACS-isolated MuSCs were snap-frozen. RNA was extracted using the Nucleospin RNA XS kit (Machery-Nagel) RNA (10 ng) was reverse-transcribed using oligo(dT) priming with the SMARTer Ultra Low Input system (Takara). The cDNA then sheared with a Covaris S2 ultrasonicator. End repair, multiplexed adaptor ligation, and 13-15 cycles of library amplification were performed using the Ovation Ultralow Multiplex system (NuGEN). Libraries underwent paired-end 101-bp sequencing at the Stanford Genome Sequencing Service Center with an Illumina HiSeq 2000 to a depth of 20-40 million reads.