title: GSM2257141: Panc1_control_mRNASeq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of cells was extracted by Trizol (Invitrogen) The RNA fragments were copied into first strand cDNA using reverse transcriptase and random hexamer primers followed by second strand cDNA synthesis using DNA Polymerase I and RNaseH. The short cDNA fragments were finally prepared for sequencing on the Illumina Genome Analyzer using reagents provided in the Illumina Genomic DNA Sequencing Sample Prep Kit. The cDNA fragments were end-repaired using T4 DNA polymerase and Klenow DNA polymerase and a single adenine nucleotide base was added to the cDNA fragments by using 3prime to 5prime exonuclease.