title: RNA-seq on DGRP lines and F1 crosses
design: RNA-seq on DGRP lines and F1 crosses
library_construction_protocol: Total RNA was extracted using the combined Trizol/RNeasy (Qiagen, www1.Qiagen.com) protocol. RNA quality was measured using RNA Labchips and Bioanalyzer from Agilent Technologies (http://www.chem.agilent.com). RNA-seq libraries were prepared using the RNA-True seq kit (Illumina).