title: DNA Pool-seq of adult female flies: F18 generation of recombination between Drosophila americana females and D. novamexicana males
design: A total of 157 females (F18 generation) were divided and pooled into four quartiles based on eye size (corrected for body size). The gDNA of the second quartile (39 flies showing intermediate/small eye size) was extracted using phenol/chloroform. Libraries were prepared using the kit TruSeq Nano DNA Library Prep from Illumina.