title: GSM2616948: Adr2-_2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was isolated from neural cells or from whole worms at the L1 stage using Trizol (Invitrogen) followed by treatment with DNase (Fischer Scientific, Hampton, NH) and purification using the RNeasy Extraction Kit (Qiagen, Hilden, Germany). RNA libraries were created from RNA isolated from three independent biological replicates of FACS sorted neural cells from the wild-type (BB76) and adr-2(-) (BB78) worm strains. PolyA+ beads (Invitrogen) were used to select for mRNA and libraries were generated using the KAPA Strand-Specific RNA Library Kit according to the manufacturer’s instructions.