title: GSM2794789: pos0a; Mus musculus; RNA-Seq
library_construction_protocol: Cells in culture were treated for 1 hour with 0, 0.5, or 4 M plumbagin dissolved in DMSO, with or without overnight and continued treatment with 10-8M DHT in DMSO, or mock treatment with DMSO. The DMSO concentration was constant between treatment conditions. After treatment, cells were harvested and RNA was purified using RNeasy (Qiagen, Germantown, MD, USA). NGS sequencing libraries were prepared from using NEBNext for Illumina® using index primers (New England Biolabs, Ipswich, MA, USA). These libraries were sequenced, 6 index primers per lane, using the Illumina HiSeq platform at the UCSD sequencing facility (http://igm.ucsd.edu/genomics). Reads were from 252-269 reads per lane. NEBNext for Illumina® using index primers (New England Biolabs, Ipswich, MA, USA Pools of 6 samples per lane using index primers