title: GSM2443355: AZA non-responder PD7158_EOT; Homo sapiens; RNA-Seq
library_construction_protocol: Bone marrow CD34+ cells were isolated from mononuclear cells from MDS or CMML patients. RNA was then isolated from 100,000 CD34+ cells using Qiagen Rneasy Mini kits as per manufacturer's recommendations. 30ng of RNA was amplified using NuGEN Ovation RNA-seq v2 kits and the resulting amplified ds cDNA was used for library preparation following standard Illumina protocol. RNA libraries were prepared for sequencing using standard Illumina protocols