title: GSM4459805: DP70; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted from samples using TRIzol (Invitrogen) following the methods by Chomczynski et al (DOI:10.1006/abio.1987.9999). 2 μg total RNAs were used for stranded RNA sequencing library preparation using Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Catalog NO. MRZG12324, Illumina) and KCTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Hiseq X 10 sequencer (Illumina) with PE150 model.