title: MNase-seq of Arabidopsis thaliana: drought stress conditions, H2A.Z-tagged line 5D
design: 10-100 ng of MNase-treated DNA samples were combined with End Repair Mix and incubated at 200C for 30 min. End-repaired DNA was purified with QIAquick PCR Purification Kit (Qiagen), then A-Tailing Mix was added and the reaction was incubated at 37oC for 30 min. The resulting mixture was combined with adapter and Ligation Mix, and incubated at 20oC for 15 min. The adapter-ligated DNA was purified with the QIAquick PCR Purification Kit. Several rounds of PCR amplification with PCR Primer Cocktail and PCR Master Mix were performed to enrich the Adapter-ligated DNA fragments. Then the PCR products were selected by running a 2% agarose gel to recover the target fragments of 150 bp. After excission from the gel, the fragments were purified with QIAquick Gel Extraction Kit (Qiagen) and validated using Agilent 2100 bioanalyzer.