title: GSM2526576: INT_Tcra_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from sorted DP thymocytes using Trizol (Life Technologies). Adapters were ligated to the 5' ends of total RNA using a template-switch RACE protocol. Tcra transcripts were amplified, and Illumina adapter sequences added, using a sense primer that annealed to the 5' RACE adapter and an antisense primer that annealed to Trac.