title: GSM3266838: Nocturnal_ZT0_repl_A; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Flies (3-4 days old) were entrained for 3 days in LD12:12 at 25°C and then male heads were collected in liquid nitrogen at ZT0 and 12. RNA was extracted using the Maxwell®16 MDx Research Instrument (Promega) combine with the Maxwell®16 Tissue Total RNA pufication kit (AS1220, Promega). The RNAseq library preparation and sequencing was carried by Glasgow Polyomics using an IlluminaNextseq500 platform.Two independent libraries paired-end were generated per time point per line