title: Transcriptom of Populus euphratica
design: Illumina Hiseq 2000 library was constructed for Solexa sequencing. Poly (A) mRNA was first enriched with oligo (dT), and then was fragmented into small pieces of 200-700 bp using divalent cations at an elevated temperature. Based on these cleaved RNA fragments, we used random hexamer-primer and reverse transcriptase (Invitrogen) to synthesize cDNA. Three paired-end cDNA libraries with an insert size of 200 bp were constructed, and then sequenced using Illumina HiSeq™ 2000 to generate an average read length of 90 bp as raw data.