title: Illumina HiSeq 2000 paired end sequencing; Gene expression profiles of null Pacman/Xrn1 mutant Drosophila L3 wing imaginal discs
design: Gene expression profiles of null Pacman/Xrn1 mutant Drosophila L3 wing imaginal discs
library_construction_protocol: null pcm mutants pcm14 and pcm15 were induced by excision of P elements from Bloomington stocks 11456 and 33263 respectively. Homo/hemizygous pcm14 and pcm15 mutants are lethal at the pupal stage and were balanced over FM7i-GFP. Homo/hemizygous pcm14 and pcm15 mutants were selected under UV light (non-GFP larvae). wild-type 1 is a clean excision of the same P-element used to create pcm14. wild-type 2 is a clean excision of the same P-element used to create pcm15. 60 L3 wing imaginal discs were dissected per biological replicate for wild-type samples. 120 L3 wing imaginal discs were dissected per replicate for pcm14 and pcm15. RNA was extracted using a Qiagen miRNeasy micro kit (217084) with on-column DNase treatment (79254) Sequencing libraries were created using an Illumina TruSeq RNA Sample Prep Kit v2