title: GSM1178909: Ski2_4; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Cell lysate Extracts from UV-crosslinked yeast cells were used to isolate RNAs cross-linked to a tagged protein in a 2-step purification protocol. Recovered RNAs are subjected to limited RNAse digest, linker ligation and cDNA amplification, and identified by Illumina sequencing (according to Granneman et al., PNAS, 2009). The 5' linkers are (mostly) barcoded - see sample description field for the sequence of the barcode for each sample.