title: GSM3577996: N2_rep2_GRO-seq; Caenorhabditis elegans; OTHER
library_construction_protocol: Synchronous populations of worms were grown at 20°C on NGM plates seeded with OP50 E. coli concentrated food at a density of maximum 40,000 animals per 15 cm Petri dish and harvested at Young Adult stage at 48 hours post hatching. The harvested animals were washed three times with M9 buffer and nuclei were extracted as described by Cecere et al., 2014. GRO-seq experiments were performed as described by Cecere et al., 2014 except that biotin-UTP was used to label nascent RNAs.