title: Pooled sample consisting of samples: Cyst 303 S1|Cyst 306 S1|Cyst 314 S1|Cyst 319 S1|Cyst 321 S1|Cyst 332 S1|Cyst 341 S1|Cyst 343 S1|Cyst 346 S1|Cyst 348 S1|Cyst 350 S1|Cyst 356 S1|Cyst 367 S1|Cyst 373 S1|Cyst 377 S1
design: Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument