title: GSM3041168: AZ9_Compound_D5_AZ65_14; Homo sapiens; RNA-Seq
library_construction_protocol: For each experiment 10-20 hCO were pooled, snap frozen and RNA extracted using Trizol (ThermoFisher Scientific), treated with DNAse (Qiagen) and purified using RNeasy Minielute Cleanup Kit (Qiagen). The quality of the RNA was assessed by Fragment Analyzer (Advances Analytical Technologies) and samples with RNA quality number ≥8.9 were used for library preparation. RNA samples were processed with Illumina TruSeq Stranded mRNA Library prep kit selecting for poly(A) taled RNA following the manufacturer's recommendations. Libraries were quantified with Qubit HS (ThermoFisher) and Fragment Analyzer (Advances Analytical Technologies) adjusted to the appropriate concentration for sequencing. Indexed libraries were pooled and sequenced at a final concentration of 1.8 pM on an Illumina NextSeq 500 high-output run using paired-end chemistry with 75 bp read length.