title: GSM2966320: Microglia 3; Mus musculus; RNA-Seq
library_construction_protocol: Tumor associated microglia and normal microglia was purified from tumor and cerebral cortex separately. In briefly, tumor mice were anaesthetized (60mg/kg ketamine; 12 mg/kg xylazine). Tumor tissue or cerebral cortex was dissected. After cutting into pieces, tissue was dissociated in enzyme solution (100 U papain, 4mg L-cysteine and 200U DNase I) for 45 minutes. Digested tumor pieces were triturated in enzyme inhibitor solution. After depletion of myelin, the microglia was incubated and captured with CD11b magnetic beads for one hour on ice. The cell suspension was added into purification column. After washing three times with washing buffer, the microglia was eluted into suspension buffer. Cells were spin down at 1000g for 5 minutes.