title: 16S rRNA V4 from soft coral tissue of Eunicea flexuosa (SampleID: E107T): with custom peptide nucleic acid clamp
design: The universal bacterial primers (515F-806R) for the 16S rRNA (V4 region) were used to amplify the microbial samples from coral tissue. A custom peptide nucleic acid (PNA) clamp (Reigel et al. 2020) was added during PCR to mitigate host DNA contamination. Samples were sequenced on a 2x251 Illumina MiSeq run.