title: GSM3043107: Lrp::kanR Minimal transition point rep1-ChIPseq input; Escherichia coli; ChIP-Seq
library_construction_protocol: RNA was harvested using Qiagen RNAProtect. RNA was isolated, and rRNA depletion was performed according to the Illumina Ribo-Zero rRNA Removal Kit for Bacteria. Specific Lrp-DNA complexes were isolated using a monoclonal antibody developed against Lrp, and DNA was isolated by phenol-chloroform extraction. DNA was preprared for sequencing using the NEBNext DNA Library Prep Kit.  RNA was prepared fro sequencing using the NEBNext Ultra Directionaly RNA Library Prep Kit.