title: GSM2445660: MSC Educated Macrophages 2; Homo sapiens; RNA-Seq
library_construction_protocol: On day +10, CD14+ cells were collected using Stem Pro Accutase cell detachment (Gibco Life Technologies) and re-sorted based on CD14 to eliminate the MSCs in the MEM cultures. Libraries were prepared to a mean size of 300-500 base pairs and analyzed using the High Sensitivity DNA Kit (Agilent Technologies) with the Bioanalyzer.  Libraries were then diluted 1:10,000 and analyzed using the Kapa Library Quant Kit (Kapa Biosystems, Wilmington, MA) on the LightCycler96 (Roche Diagnostics, Indianapolis, IN). 