title: GSM2446197: Axenic replicate 2, 13:11 day:night cycle -> extended darkness, Time 144; Prochlorococcus marinus str. NATL2A; RNA-Seq
library_construction_protocol: Culture samples added to cold RNAlater at each timepoint, cells collected onto a 0.2µm filter, and total RNA extracted with the mirVana kit (Ambion). Strand-specific RNA-seq library construction starting with 100ng of total RNA using the dUTP second-strand approach; duplex-specific normalization was performed to reduce ribosomal RNA content in each sample.