title: GSM4460113: NTT_Braf/Pten_4SU_2h_DMSO2; Mus musculus; RNA-Seq
library_construction_protocol: Fro SLAM-Seq cells were pre-treated with DMSO or MEKi for 30 min, then 4-SU was added and cells were harvested by snap-freezing cells after 90 min of labelling. RNA was extracted using RNAEasy mini kit (Qiagen), total RNA was alkylated and ethanol purified. Libraries were generated using QuantSeq library prep kits from Lexogen (QuantSeq 3' mRNA-Seq Library Kit) using 500ng of RNA, according to manufracturers instruction. QuantSeq