title: GSM2027826: WT female 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol reagent (Life technologies), treated with DNase I (Roche) and then purified using the RNeasy Mini Kit (Qiagen). RNA integrity number (RIN) was measured using a 2100 Bioanalyzer (Agilent Technologies). Purified RNA with RIN greater than 8 served for preparation of poly(A)+ RNA Seq libraries using the mRNA-Seq Sample Preparation Kit (Illumina).