title: GSM4460506: C61A_iso_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using Rneasy Mini kit (Qiagen) Total RNA was quality-checked using RNA Bioanalyzer (Agilent) Nano chip and subjected to ribosomal RNA depletion using TruSeq (Illumina, San Diego, CA). Libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) and sequenced using the NextSeq 500 system (Illumina, San Diego, CA) at a minimum depth of 20 million single-end 75 bp reads per sample.