title: GSM3204815: tumor1_IP; Homo sapiens; RIP-Seq
library_construction_protocol: Total RNA from cells in culture was extracted using Trizol reagent (Thermo Fisher Scientific, Waltham, MA), and then treated with DNase I (04 716 728 001, Roche Diagnostics, Indianapolis, IN) for 20 min at 37°C to remove DNA contamination. RNA libraries were prepared  using SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian.