title: WGS of Pectobacterium brasiliense: strain IPO:4132 NAK:239
design: Libraries for Illumina sequencing  were constructed on randomly sheared DNA fragments (Covaris E210) aiming 300  to 500 bp insert size using the NEXTflex ChIP-seq Library prep kit (BIOO  Scientific). Library sizes were analyzed on a Bioanalyzer (Agilent) and  quantified using Qubit (Life Technologies) equimolar pooled. The library pool  was loaded on an Illumina paired-end flowcell for cluster generation using a  cBot (illumina). Sequencing was done on an Illumina HiSeq2500 instrument  using 125, 7, 125 flow cycles for forward, index and reverse reads  respectively