title: Pooled sample consisting of samples: Cyst 160 S3|Cyst 166 S3|Cyst 181 S1|Cyst 182 S1|Cyst 183 S1|Cyst 184 S1|Cyst 185 S1|Cyst 186 S1|Cyst 187 S1|Cyst 188 S1|MAX 024 S2
design: Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument