title: GSM2255738: S6_F1; Danio rerio; ncRNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer’s procedure. The total RNA quantity and purity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 1 μg of total RNA was used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Then, we performed the single-end sequencing (36 bp) on an Illumina HiSeq 2500 at the LC-BIO (Hangzhou, China) following the vendor’s recommended protocol.