title: GSM4459725: Sle1b Mouse 1; Mus musculus; RNA-Seq
library_construction_protocol: B cells were sorted from a single splenocyte suspension on a BD FACS Aria sorter.  RNA was extracted from B cells using a combination protocol.  Cells were lysed with Trizol reagent and processed until ethanol extraction, at which point samples were loaded onto Qiagen RNeasy columns and the remainder of the Qiagen isolation protocol was followed. Libraries were prepared following the Nextera XT library preparation protocol