title: GSM2092581: Blimp1-Bio ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: phenol-chloroform extraction About 1-5 ng of cDNA or ChIP-precipitated DNA were used as starting material for the generation of sequencing libraries with the NEBNext Ultra Ligation Module and NEBNext Ultra End Repair/dA-Tailing module. DNA fragments of 200-500 bp were selected with AMPure XP beads (Beckman Coulter). PCR amplification was performed with the KAPA Real Time Amplification kit (KAPA Biosystems). Completed libraries were quantified with the Agilent Bioanalyzer dsDNA 1000 assay kit and Agilent QPCR NGS Library Quantification kit. Cluster generation and sequencing was carried out by using the Illumina HiSeq 2000 system with a read length of 50 nucleotides according to the manufacturer’s guidelines.