title: : 16S rRNA amplicon sequencing of mus musculus: fecal
design: Library construction and sequencing was performed in a 96-well                 multiplexed format by the University of Missouri DNA Core. In brief, dual-indexed                 universal primers F515/R806 , flanked by Illumina adaptor sequences, were used to                 construct amplicons of the V4 hypervariable regions of 16s rRNA gene. Amplicon                 libraries were pooled and sequenced on the Illumina MiSeq platform and the V2                 chemistry.