title: GSM3266873: WD 0weeks, D; Mus musculus; RNA-Seq
library_construction_protocol: HSCs were lyzed in RLT lysis buffer from RNeasy Mini Kit (Qiagen) and total RNA extracted according to manufacturer's instructions after a standard phenol/chloroform extraction. RNA concentrations were measured using a Qubit 3.0 Fluorometer (Invitrogen) and quality of the RNA was assessed using a Bioanalyzer 2100 (Agilent Technologies). Purified RNA (3-4 biological replicates) was polyA-selected and subjected to fragmentation before cDNA synthesis. For library construction, we used the NEBNext Ultra™ RNA Library Prep Kit for Illumina and sequenced using the Illumina HiSeq 1500 platform.