title: GSM2027032: KPC pancreatic fibroblasts, biological replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions. Poly-A containing mRNA molecules was purified from total RNA (0.6 µg; RIN >7) using poly-T oligo-attached magnetic beads from TruSeq RNA Sample Prep Kit v2 (Illumina; RS-122-2001). Subsequent library construction of the RNA-seq library according to the manufacturer's instructions. Libraries were prepared with 13 cycles of PCR. Sequencing was performed using the single-read 50bp approach on HiSeq 2000 high output mode.