title: Illumina HiSeq 2000 sequencing; Pol III ChIP-seq mouse development
design: Pol III ChIP-seq mouse development
library_construction_protocol: C57BL/6J mice were bred and housed under UK Home Office licensing husbandry standards. After breeding, mice were observed for plugs. Morning of day when plug was first observed was counted as day E0. Samples were prepared by direct perfusion of the tissue with buffered salt solution, followed by 1% formaldehyde cross-linking. After 20 minutes, the cross-linking reaction was quenched with 1/20 volume of 2.5 M glycine to neutralize the formaldehyde. The animal tissues were crosslinked with formaldehyde and chromatin fragmented to an average of 300 bp by sonication. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the inst