title: Deciphering the astrovirus and norovirus diversity in wastewater treatment plant effluents by high-throughput sequencing method.
design: The goal of this study was to monitor the seasonality of human astroviruses and noroviruses in WWTP (wastewater treatment plant) effluents by high throughput sequencing. One specific MID (multiplex identifier) was selected in order to discriminate each season (Spring: ACGCTCGACA; Summer: AGACGCACTC; Autumn: AGCACTGTAG; Winter: ACGAGTGCGT); Human astrovirus primers (Forward primer 5'-3'(MON269): CAACTCAGGAAACAGGGTGT; Reverse primer 5'-3'(MON270): TCAGATGCATTGTCATTGGT); Human NoV GI (Forward primer 5'-3' (G1SKF): CTGCCCGAATTYGTAAATGA; Reverse primer 5'-3'(G1SKR): CCAACCCARCCATTRTACA); Human NoV GII (Forward primer 5'-3'(G2SKF): CNTGGGAGGGCGATCGCAA; Reverse primer 5'-3' (G2SKR): CCRCCNGCATRHCCRTTRTACAT).