title: GSM4698451: NC1; Homo sapiens; ncRNA-Seq
library_construction_protocol: We isolated EXOs from all of the cultured neutrophil supernatants (neutrophil EXOs) using an exoEasy Maxi Kit (Qiagen, Frederick, USA) according to the manufacturer's instructions. The neutrophil EXOs were eluted using 600 μl Buffer XE and stored at −80 °C until analysis. The total RNA of neutrophil EXOs was extracted with TRIzol (Invitrogen Life Technologies, California, USA) according to the manufacturer's protocol. Small RNA and lncRNA libraries were created with an NEBNext® Multiplex Small RNA Library Prep Set for Illumina (NEB, Ipswich, USA) and an NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB), respectively, according to the manufacturer's instructions.