title: GSM3656391: Control_1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA from plants treated with chemical was isolated using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's protocol and treated with RNase-Free DNase Set (Qiagen), followed by column purification using Rneasy MiElute Cleanup kit (Qiagen). Library construction and sequencing for the Illumina HiSeq 2500 was provided as a custom service of Eurofins Genomics K. K. (Tokyo, Japan). The polyA fraction (mRNA) was isolated from total RNA, followed by its fragmentation.Then double-stranded (ds) cDNA was reverse transcribed from fragmented mRNA. The ds cDNA fragments were processed for adaptor ligation, size selection (for 200 bp inserts) and amplification to generate strand-specific cDNA libraries.Prepared libraries were subjected to paired-end 2 x 100 bp sequencing on the HiSeq 2500 platform with v4 chemistry.