title: GSM1179662: Restricted 6 tp2; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA was extracted from frozen muscle tissue by homogenisation in Trizol. RNA was then DNase treated and purified using QIAquick PCR spin columns. Standard Illumina paired-end mRNA-seq libraries were constructed from cDNA which was made from mRNA which had been purified from total RNA by two rounds of polyA tail selection using oligo dT beads.