title: GSM2616703: FARI_1511_H5; Homo sapiens; RNA-Seq
library_construction_protocol: Sorted Tregs and CD154+ T cells were resuspended and loaded onto a Fluidigm C1TM Single-Cell Auto Prep IFC for Fluidigm C1TM processing as per manufacturer protocols (Fluidigm). Single-cells were visualized by microscopy and wells containing >1 cell were noted and excluded from further steps. cDNA was generated according to Fluidigm C1TM protocols with SMARTer® UltraTM Low RNA Kit for the Fluidigm C1TM System reagents (Clontech Laboratories). Resultant cDNA from Fluidigm C1TM processing was subsequently assessed for quantity and quality using a Qubit Fluorometer 3.0 (Thermo Fisher Scientific) and 2100 Bioanalyzer or 2200 TapeStation (Agilent Technologies). cDNA which passed QC were subsequently processed for library construction using the Nextera XT DNA Library Prep and Nextera XT Index Kits (all from Illumina).