title: GSM3268014: Mo_Veh_4C_IRAK3; Homo sapiens; OTHER
library_construction_protocol: Monocytes were fixed with formaldehyde and then lysed. Isolated nuclei were then fully digested by DpnII followed by overnight ligation. Ligated cycle DNA were purified after de-crosslinking incubation with proteinase K at 65 degree for 4 hours, followed by further digestion by NlaIII. The digested DNA were ligated again and purified. The 4C library was amplified with the viewpoint specific primers with inverse PCR. For each viewpoint, 8 PCR reaction products were pooled to enhance the library complexity. Kapa hyperprep kit.