title: GSM4459452: MCF7_D538G_clone1_DMSO_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested from 100mm dishes in 600mL RLT Plus (Qiagen) supplemented with 1% beta-mercaptoethanol after the induction with 10nM E2 or DMSO. RNA purification was performed using RNA Clean and Concentrator (Zymo Research) with the optional DNase I treatment. The KAPA Stranded mRNA-seq kit (Kapa Biosystems) was used to create Poly(A) selected libraries from 500ng total RNA.