title: 16S rRNA-Seq: Soil bacterial community
design: Five gram of soil was added in 100 mL diluted nutrient broth (0.8 g/L) to make a slurry. One milliliter of slurry was sampled after 67 hour's incubation at 30 oC, and the solid phase was extracted the genomic DNA and submitted to 16S rRNA gene Illumina sequencing.