title: GSM3267399: Mouse LGN snRNAseq 0 -8  hour stim rep 1-4 (12 samples); Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized following dark-rearing between P20 and P27 then re-exposure to light for 0, 1, or 8 hours. The LGNs from 3-4 mice of each condition were pooled to represent one bioreplicate, and four bioreplicates were included in the analysis. After isoflurane anesthetization, mice were decapitated and the brain was isolated. Mouse brains were dissected and 300 um coronal sections were made on a Leica VT1000S vibratome. The dorsal LGNs were then microdissected in ice cold PBS after visual identification using a Nikon SMZ-10A brightfield dissection microscope, and flash frozen. Individual cells were captured and barcoded using the inDrops platform as previously described. Briefly, single-cell suspensions were fed into a microfluidic device that packaged the cells with barcoded hydrogel microspheres and reverse transcriptase/lysis reagents. After cell encapsulation, primers were photo-released by UV exposure. Two libraries of approximately 3000 cells each were collected for each sample. Indexed libraries were pooled and sequenced on a Nextseq 500 (Illumina). single-nucleus RNA-sequencing