title: GSM3044880: FO-IgD-Lo-B-cell-080515; Mus musculus; RNA-Seq
library_construction_protocol: N/A Total splenic B cells from 8-10 wk old mice were purified by negative selection using magnetic cell sorting (STEMCELL Technologies, Vancouver, BC, Canada) followed by sorting of specific subsets of B cells using FACS.  B cell purities were ~ 99% as determined by flow cytometry. Total RNA was isolated from sorted B cells using RNAqueous® −4PCR kit (Ambion, Austin, Tx). Illumina kit, single end, not strand specific.