title: GSM4459042: DUX_OE-1_ASTAR-RNA-30; Mus musculus; RNA-Seq
library_construction_protocol: Cells were trypsinized and single cell suspensions were harvested for ASTAR Scripts run using C1 Fluidigm Open App chips (10-17um). The concentration of amplified cDNA was measured by high sensitivity bioanalyzer chips and diluted to required range of cDNA for following library preparation steps. ASTAR-mRNAseq libraries were prepared using Nextera XT DNA Library Preparation Kit (FC-131-1096) following “Using C1 to Generate Single-Cell cDNA Libraries for mRNA Sequencing” manual. Single-cell RNA-Seq