title: WGS of drosophila melanogaster: female adult
design: For Illumina short-read sequencing, the whole body of 3-5 day old female virgin flies (ISO-1 strain, ~25 individuals) were collected and used for DNA extraction with SDS. DNA quality was assessed by OD260/OD280 with Nanodrop and agarose gel electrophoresis. The library for Illumina sequencing was prepared as follows. (1) Fragmentation with Covaris ultrasonicator, (2) End-repair and phosphorylation of the 5 ends, (3) A-tailing of the 3 ends, (4) Ligation of adapters, (5) PCR for several cycles to enrich for ligated product. Sequencing was done with Illumina HiSeq-2500 sequencer (run type: paired-end; read length: 125 nt) in Novogene (Tianjin, China).