title: cDNA PGM sequencing of Paracoccus RNA, generation 702, C-limited
design: RNA was extracted from a pellet of 2 ml C-limited chemostat culture, generation 702, as described in Smith CJ et al. (2007) with minor modifications. Extracted RNA was treated with DNase (Promega) and purified using RNeasy MinElute spin columns (Qiagen). Ribosomal RNA was depleted from 5 μg total RNA using the Ribo-ZeroTM rRNA Removal Kit (Bacteria) (epicentre). Barcoded ion torrent cDNA libraries were prepared following the Ion total RNA Seq Kit V2 manual (Life Technologies).