title: Small RNA-seq (miRNA-seq) of primary human cells
design: RNA was isolated using the miRNeasy kit from Qiagen according to the manufacturers protocol. RNA integrity was assessed using Agilent BioAnalyzer and the RNA concentrations were measured using a NanoDrop 2000 UV-Vis Spectrophotometer. Small RNA libraries were prepared using the Illumina TruSeq Small RNA Library Preparation kit according to the manufacturers protocol, but purified using a Pippin Prep with a 3% Agarose Gel Cassette (Sage Science) and a size selection of 122-157bp. Multiplexed sequencing was performed as single read 50 base pair, using rapid run mode, and v2 chemistry on a HiSeq2500 system.