title: GSM3204770: WT_L4440_rep1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: L1s were lysed in 750μl Trizol® and snap-frozen in liquid nitrogen. After freeze cracking of the samples by 6 subsequent transfers from liquid nitrogen to 42°C, samples were let vigorously shake 15 min at RT. Total RNA was purified using RNeasy kit (QIAGEN), including DNase treatment. RNA-seq libraries were obtained using the Illumina ScriptSeq Complete Gold kit according to manufacturer's guide, starting with 5µg of total RNA as input