title: Eucalyptus urophylla vs Eucalyptus grandis Transcriptome:regular N fertilization
design: RNA extraction The total RNA was extracted according to Le Provost et al. [66]. RNA quantity and quality was checked with a Nanodrop ND-1000 Spectrophotometer (Thermo) and 2100 Bioanalyzer (Agilent).  Illumina sequencing  The mRNA-seq libraries were prepared at the High Throughput Sequencing Facility at Center of Genome, University of North Carolina, USA, using 10 μg of total RNA according to the Illumina’s protocol instructions, following a room temperature gel extraction step to avoid under-representation of AT-rich sequences [67]. The quality and quantity of each library were verified by Bioanalyzer Chip DNA 1000 series II (Agilent). For each library, one lane of an Illumina Genome Analyser IIx was used to produce the 36 bp single-end sequences.