title: Whole genome sequence of vaccine strain of Bordetella pertussis strain 165 isolated from nasopharyngeal aspirate of the patient
design: Genomic DNA was extracted and purified using Genomic DNA Clean and Concentrator -10Kit (ZYMO Research) as per the manufacturers instructions. Whole genome sequencing was performed using Illumina MiSeq platform (Illumina, San Diego, CA, USA). The MiSeq shotgun library was prepared using NEBNext UltraTM DNA Library Prep Kit and was analyzed in Bioanalyzer (Agilent Technologies) using 1000 Chip as per the manufacturers instructions followed by 150x2 paired-end sequencing.