title: B04_480ppm, Soil type structures fungal community responses to past and future atmospheric CO2 concentrations
design: We studied fungal communities in a grassland ecosystem exposed to a preindustrial-to-future CO2 gradient (250-500 ppm) on two contrasting soil types, a black clay and a sandy loam.  Pyrosequencing of rDNA revealed that fungal community composition and its response to CO2 differed significantly between soil types.  We used the fungal-specific primer ITS1f (Gardes and Bruns 1993) and the general-eukaryotic primer ITS2 (White et al. 1990) to amplify the first internal transcribed spacer region of the rRNA gene.  Primers included a unique 10 nt barcode for each sample.  The amplicons, about 350-400 bp long, were sequenced unidirectionally from the ITS1f primer on a Roche 454 GS-FLX system using Titanium and LibL chemistry (454 Life Sciences, Branford, CT).