title: GSM3889045: PS04-4; Streptomyces coelicolor; RNA-Seq
library_construction_protocol: Mycelia were collected by centrifugation, frozen and stored at -70°C for subsequent RNA isolation. RNA was isolated using the procedure described previously by Moody et al. (Moody et al., 2013), digested with TURBO DNase I (Invitrogen, USA) and checked for chromosomal DNA contamination using PCR. RNA libraries were prepared for sequencing using standard Illumina protocols (NEBNext Ultra II Directional RNA Library Prep Kit)