title: GSM4648457: arid4b∆ Endoderm H3K4me3; Mus musculus; ChIP-Seq
library_construction_protocol: Formaldehyde fixed lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Chromatin Immunoprecipitation (ChIP) was performed as described previously (Kim et al., 2009; Das et al., 2014). ChIP-seq libraries were prepared using NEBnext ChIP-seq library kit (E6240S) and NEBnext multiplex oligos fo Illumina sequencing (E7335S) according to manufacturer's protocol. The concentrations of library cDNA samples were analyzed using Qubit. Sequencing was performed using Illumina HiSeq2000.