title: GSM4587115: Control 5; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from individual corneas by homogenization in 1 ml  TRIzol Reagent (Life Technologies -15596–026) at room temperature and  chloroform-extracted. The RNA in the aqueous phase was precipitated using  0.5 ml of 100% isopropanol and collected by centrifugation at 12,000 g,  washed with 75% ethanol and resuspended in RNAse-free water. TruSeq stranded RNA library kit construction protocol