title: GSM2027502: GcsR_rep1; Pseudomonas aeruginosa; RNA-Seq
library_construction_protocol: Cultures were stabilized by adding 1 mL of RNAprotect Bacteria reagent (Qiagen) to 0.5 mL of culture.  Cells were then lysed using lysozyme and proteinase K as described in the manufacturer’s protocol. The total RNA from the lysed cells was subsequently purified using the RNeasy mini kit (Qiagen) with an on-column DNase digestion step. RNA libraries were prepared for sequencing using protocols recommended by Illumina