title: GSM4699022: 1X OS 12h replicate 2; Monodelphis domestica; RNA-Seq
library_construction_protocol: Cells were collected using Accutase (BD Biosciences) and RNA was extracted using the Ambion PureLink RNA mini kit (ThermoFisher) at 12, 48, and 96 h after transferring cells to OSS and at 0, 48, and 96 h from cells maintained under static conditions. RNA from three experiments was combined to create each sample, and three independent samples were sequenced for each time point. Library preparation was performed using the TruSEQ Stranded Total RNA Sample Preparation Kit (Illumina) according to manufacturer's instructions. Following removal of ribosomal RNA, the remaining RNA was fragmented for 8 min, followed by reverse transcription performed. Double stranded cDNA was subjected to 3' adenylation and ligation of sequencing adapters. Sequencing was carried out on a NextSeq 500 (Illumina) to generate 75 bp paired-end reads.