title: GSM1185396: sRNAs from SINV-infected HEK293 cells, treated with NaIO4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from non-infected and infected cells using Tri-reagent (Ambion) as per manufacturer’s instructions. Small RNA libraries were prepared from 10 or 20 μg of total RNA as previously described (Pfeffer, S. 2007. Identification of virally encoded microRNAs. Methods Enzymol. 427:51-63), except that PCR products were not concatemerized but directly sent for large-scale sequencing. Libraries from samples 5 to 10 were generated using the same protocol except that degenerate 5' and 3' adapters were used to limit ligation biases.