title: GSM2794341: WT CL316,243 rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from BAT using TRIzol Reagent (Life Technologies). Following separation using chloroform, the upper aqueous phase containing RNA was mixed with equal volume of 70 % EtOH, and transferred to an RNeasy spin column (QIAGEN). RNA isolation was completed using the RNeasy mini kit according to manufacturer's protocol (QIAGEN), and RNA was treated with DNase I (Zymo Research). RNA libraries were prepared from 1 µg of total RNA using the TruSeq total RNA Sample Preparation Kit version 2.0 Kit (Illumina) following the manufacturer's instructions