title: Prokaryotic 16S rRNA gene V4-V5 region amplicon of RRV9 soil
design: PCR amplicon libraries were prepared by combining the three independent PCR products for each sample. After purification using a SanPrep DNA purification kit (Sangon, Shanghai, China), the PCR products from the different samples were quantified using the Agilent 2100 Bioanalyzer System (Santa Clara, CA, USA) and then pooled at equal concentrations. Amplicon sequencing was performed on a GS FLX Titanium platform (454 Life Sciences, Branford, CT, USA) at Beijing Yuanquanyike Biological Technology Co., Ltd. (Beijing, China)