title: GSM2966344: VM CD8+ cells timestamped at day 28, moved to congenic recipients at 8 wks, collected 5 dpi (Listeria), rep 1; Mus musculus; RNA-Seq
library_construction_protocol: CD8+ cells were magnetically enriched with CD8 microbeads and sorted to > 95% purity with a FACS Aria III on CD4- CD8+TdTomato+. Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. Directional RNA-seq libraries were prepared from 25 ng total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.