title: Exome seq of cell line CO:HCT_116
design: 37Mb of coding region for each cell line was captured using the Agilent SureSelect All Exon v1.0 kit (Agilent). Genomic DNA (3g) was sheared using the Covaris S2 ultra-sonicator (Covaris) using the settings Duty Cycle 10%, Intensity 5%, Cycle/burst 200 and Time 60s, which yielded a fragment size distribution with a mean at 200bp. Libraries were generated using standard Illumina library protocol (Illumina) followed by size selection using ChromaSpin TE200 spin columns (Clonetech). Pre- and post-capture steps were performed following the manufacturers protocol (Agilent). The samples were sequenced as paired-end 80-mer reads on an Illumina Genome Analyzer IIx instrument (Illumina) following the manufacturers protocol.