title: GSM4586642: Rat P0 DRG neuron cells_rep1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA from cultured DRG neurons was extracted with Trizol Reagent (Invitrogen) according to the manufacturer's protocol. DRG neurons were cultured 48h in 35 mm dish before rinsed twice with ice-cold PBS,  0.5 mL Trizol was added to the dish for total RNA extraction. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB) Poly(A)+ RNA-seq