title: GSM4698245: AMut1_S14; Danio rerio; RNA-Seq
library_construction_protocol: Zebrafish grna-/- and grna+/+ control siblings were anesthetized in tricaine, subjected to cardiocentesis and kidney dissection as previously described (Traver et al., 2003). The resulting kidney suspension was gently triturated with a P1000 pipette and filtered with a 30µm cell strainer. The cell suspension was lysated in RLT buffer and total RNA extraceted using RNeasy (Qiagen) following the manufacturer instructions. Total RNA was assessed for quality using an Agilent Tapestation 4200, and samples with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep (Illumina, San Diego, CA). Samples were processed following manufacturer's instructions, starting with 50 ng of RNA and modifying RNA shear time to five minutes.