title: small-RNA set: Anopheles gambiae midgut-biol rep 3
design: After midgut dissections, RNA was isolated by Directzol RNA miniprep kit, and 1  g of total RNA from these tissues was used to make the small RNA library. Small RNA libraries were prepared using the Illumina TruSeq Small RNA Sample Preparation Kit and ligated to Small RNA TruSeq index adapters by reverse transcription. Size selection of the cDNA library constructs was performed with The Pippin Prep size fractionation system and purified by the Agilent 2100 Bioanalyzer was used with the High Sensitivity DNA Kit. The small RNA libraries were run on the HiSeq2500 in Rapid Read mode with a 1x50 cycle read and index read using the TruSeq Rapid SBS kit-HS. Post collection, sequence data was converted to FASTQ files and de-multiplexed into individual sequences for further downstream analysis.