title: GSM4462210: Arrays_DMSO; Homo sapiens; RNA-Seq
library_construction_protocol: CRC organoids grown in Organoid arrays or Matrigel® drops exposed to 1μM afuresertib or vehicle were lyzed and the mRNA was extracted using the RNeasy mini kit according to the supplier's information. The different samples were quality controlled using a TapeStation 4200 (Agilent) and sequenced using an Illumina NextSeq500. An average of 41Mio reads per sample was uniquely aligned to the hg38 Human reference genome (GRCh38) with STAR (version 2.5) and about 90% of those were confidently assigned to genes (gencode primary assembly v27) using HTSeq-count (version 0.9). Genes with less than 50 reads in all 4 samples were excluded and the log2 normalised CPM values of the remaining 13'668 genes were calculated using the R package edgeR (version 3.14). RNA libraries were prepared for sequencing using standard Illumina protocols