title: GSM1834036: LML_P1; Ovis aries; RNA-Seq
library_construction_protocol: Total RNA was isolated from mammary tissue samples using Trizol (Invitrogen) and purified using RNeasy mini kit (Qiagen). Genomic DNA contamination was eliminated via on-column digestion with DNase (Qiagen), as per the manufacturer’s protocol. The concentration and quality of RNA was measured using a Nanodrop ND-1000 spectrophotometer (Nanodrop) and integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). TruSeq RNA sample prep kit – V2