title: GSM4586384: Dmel-ISO1-Pacbio-sample2-SMART2; Drosophila melanogaster; OTHER
library_construction_protocol: Female virgin flies (ISO-1 strain, ~180 individuals for each of two samples) were collected and starved for 1.5 h and flash-frozen in liquid nitrogen. Genomic DNA was extracted and purified with standard procedures. The DNA library preparation for PacBio sequencing was performed following the Procedure & Checklist of 20 kb Template Preparation Using BluePippin Size-Selection System. Briefly, the DNA was sheared by a Covaris g-TUBE device and purified with AMPure PB beads. The fragmented DNA was subject to DNA damage repairing and ligated with adapters. Then purified ligation products were size selected by the BluePippin Size Selection system. After annealing and binding of SMRTbell templates and preparation for MagBead loading, the two libraries were run on the PacBio RS II and Sequel system in NextOmics (Wuhan, China), respectively; and the sequencing results for each sample were composed of two SMART cells.