title: GSM3656432: shS6K2 cells with sh1: TRNC0000000729, replicate 1 [shS6K2.sh1.1]; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in Tri-Reagent (Sigma-Aldrich, #T9424-100ml) and RNA was extracted using the Direct-zol MiniPrep Kit (Zymo Research, #R2050) with in column DNAse I treatment. Total RNA (200ng) was used to prepare 3' QuantSeq mRNASeq libraries (Lexogen, #KO152x96)