title: GSM4698781: MYB134RNAi-24-3; Populus tremula x Populus tremuloides; RNA-Seq
library_construction_protocol: Leaves of wild type and transgenic poplars were harvested. Total RNA was extracted from frozen poplar hairy roots or ground leaf powder using the modified cetyl trimethyl ammonium bromide method for polyphenol-rich plant tissue described by Muoki et al. (2012). MagJET enrichment kit (ThermoScientific) was used to purify mRNA from crude RNA. The mRNA was fragmented by heating the samples at 94℃ for 5 minutes. To make cDNA libraries with adaptors NEBNext ultra RNA Library Prep Kit for Illumina (NEB) was used. GeneJET NGS Cleanup Kit (ThermoScientific) was used to clean the extra adaptors. Primers for RNA-Seq were added to cDNA using NEBNext Multiplex Oligo kit (NEB). MagJET NGS Cleanup and Size Selection kit (ThermoScientific) was used to select DNA fragments around 350bp. Twelve libraries with same concentration (250ng/ul) each were pooled and sent to McGill University/GenomeQuébec.