title: GSM2026283: NSC_iPS_p3_2 Hi-C; Mus musculus; OTHER
library_construction_protocol: Cells are cross linked using 2% formaldehyde for 10min at room temperature in PBS 10%FCS, nuclei are isolated, after which chromatin is digested with DpnII subsequently ligated. After reversal of the cross links the DNA is purified (3C template). The 3C template is fragmented and TruSeq libraries are prepared according to the vendor protocol.