title: GSM4648571: glob-micropylar-Input replicate 2; Phaseolus coccineus; ChIP-Seq
library_construction_protocol: Chromatin isolation and immunoprecipitation were performed as described in Gendrel AV, Lippman Z, Martienssen R, Colot V. Nat Methods. 2005;2:213–218, except that the isolated nuclei were resuspended in lysis buffer described in by Johnson et al. (2002) and sonicated to achieve chromatin fragments ranging from 100 to 500 bp. Nuclear extracts were either set aside for whole genome extracts (input control) or incubated overnight with 10 ug of an antibody raised against the peptide PNSPTTSVNQTYFQ corresponding to the amino acids 176-190 of Scarlet Runner Bean WOX9-like protein (GenBank No. ACL11801). Crosslinks were reversed and protein digested as described in Dahl and Collas, Nat Protoc. 2008;3(6):1032-45, extracted with phenol:chloroform and precipitated using standard procedures. ChIP and Input libraries were generated using Nugen Ovation Ultralow System V2, following the manufaturer's instructions and enriched by PCR using 15 cycles.