title: Comparative Transcriptome Analysis of Pre- and Post-Climacteric Fruit of Chinese Pear (Pyrus ussuriensis) Reveals New Insights into Fruit Ripening
design: Pear fruit were harvested at commercial harvest day (0), and then stored at room temperature for 10 d (10). mRNAs were extracted from smaple 0 and 10. First-strand and second-strand cDNAs were synthesized and purified. The purified cDNAs were subjected to end reparation and polyadenylation and were mixed with Solexa adapters. Suitable fragments were recovered from an agarose gel and amplified by PCR, and they were then sequenced using an Illumina HiSeqTM 2000. Reads from sample 0 refer to Run RNA seq, and those from sample 10 refer to Run RNA seq-2.