title: GSM4648758: AEC-1-AEC-1-5_FKDL202561884-1a-5; Mus musculus; RNA-Seq
library_construction_protocol: Morphologically deformed cells were discarded. Living single cells were rinsed in PBS-BSA, and manually transferred into cell lysis buffer with a mouth pipette; 0.05 μl of 1:200,000 dilution of External RNA Controls Consortium (ERCC) RNA spike-in Mix1 (Ambion) was added to lysis buffer per reaction. The cDNA libraries from single cells were generated as described previously. "Tang, F. et al. mRNA-Seq whole-transcriptome analysis of a single cell. Nature  Methods, 6, 377–382 (2009)."