title: GSM4698188: H-2Kb+ TCRb+ transferred CD226-/- TIGIT-/- Treg cells and wild-type Tconv cells; Mus musculus; RNA-Seq
library_construction_protocol: Freshly isolated spleens were gently rubbed back and forth applying slight pressure with slide glass to disperse spleen cells. The cell suspension was filtered through a 70 µm cell strainer and was treated with ACK lysis buffer to lyse red blood cells. PI- H-2Kb+ TCRb+ donor T cells were sorted by flow cytometry. Sorted donor T cells were stained with barcode sequence-conjugated anti-mouse CD45.1 (TotalSeq A0178, BioLegend) and CD45.2 (TotalSeq A0157, BioLegend). 10X Genomics, Chromium Single Cell 3' Library and Gel Bead Kit v3.  BioLegend, TotalSeq™-A Antibodies with 10x Single Cell 3' Reagent Kit v3.