title: GSM2792628: TruSeq70_input_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Cell-type specific RNA from livers of P30 mice was obtained using the RiboTag protocol. Briefly, liver tissue expressing HA-tagged ribosomes in specific cells was homogenized followed by immunoprecipitation of the tagged ribosomes using an HA antibody. The RNA was extracted from the immunoprecipitated ribosomes to obtain cell-type specific enriched transcripts (IP samples). An aliquot of the homogenized tissue before immunoprecipitation was used for total RNA extraction (input samples). Libraries were prepared from 0.25ng/4ng/70ng using NuGEN Ovation RNA-Seq system V2, TaKaRa SMARTer Stranded Total RNA-Seq Kit , TaKaRa SMART-Seq v4 Ultra Low Input RNA kit , Illumina TruSeq RNA Library Prep Kit v2 and NEBNext® Ultra™ Directional RNA Library Prep Kit,  per manufacturer's instructions, with minor modification for NuGEN prepared samples. RNA libraries were prepared for sequencing using standard Illumina protocols.