title: GSM2525960: Steer 458; Bos taurus; RNA-Seq
library_construction_protocol: longissimus dorsi muscles were removed, flash frozen on liquid nitrogen and dry ice. Total RNAs were isolated using Trizol (invitrogen) and a RNeasy RNA purification kit with DNase treatment (Qiagen). mRNA was isolated from the total RNA using oligo-dT beads and were reverse transcribed into double strand cDNA fragments. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 5 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using  Illumina NextSeq 500 protocols