title: Illumina HiSeq 2500 paired end sequencing; RNA-seq analysis of branchial arches and outflow tract of the mouse embryo at E10.5 and E11.5
design: RNA-seq analysis of branchial arches and outflow tract of the mouse embryo at E10.5 and E11.5
library_construction_protocol: CD1 mice were time-mated to obtain branchial arches and OFT. Branchial arches and OFT were dissected and snap frozen in dry ice.  RNA was extracted using Trizol (Invitrogen, 15596-018), pooling 3-5 pairs of BAs for each sample. RNA-seq was performed in duplicates.  Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina)