title: The complex patterns of quasispecies in the reverse transcriptase region of hepatitis B virus in Telbivudine treatment
design: Library preparation was carried out using the Ion Xpress™ Plus Fragment Library Kit (Cat. no. 4471269, Pub.no. MAN0009847, Rev. C) with 100 ng of HBV DNA. Adapter ligation, size selection, nick repair and amplification were carried out according to the manufacturer's protocol. Sample Emulsion PCR, emulsion breaking and the enrichment steps were carried out using the Ion PGM™ Template OT2 400 Kit and associated protocol (Cat. no. 4479878, Pub. no. MAN0007218, Rev. A) according to the manufacturer's instructions. Briefly, an input concentration of template-positive Ion PGM™ Template OT2 400 Ion Sphere™ Particles (ISPs) was added to the emulsion PCR master mix and the emulsion was generated. After enriching template-positive ISPs, sequencing was undertaken using Ion 318™ Chip v2 in the Ion Torrent PGM System. The Ion PGM™ Hi-Q™ Sequencing Kit was used for all sequencing reactions according to the protocol (Cat. no. A25592, Pub.no. MAN0009816, Rev. D).