title: GSM4698762: At_whole_root_RNA; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Libraries were prepared for sequencing using Ilumina Truseq kit. For single-nuclei samples, roots were chopped and filtered before nuclei were purified by FACS. For bulk samples, total RNA was extracted from root tips using RNeasy Plant Mini kit (Qiagen). For the whole root sample, root tips were ground directly into the Eppendorf tube by pestle. For the protoplast sample, protoplasts were isolated from root tips and then ground protoplasts by pestle.