title: GSM4586438: Palmera Control 1 [C11]; Capra hircus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the tissue using the E.Z.N.A. Total RNA Kit II (Omega Bio-Tek Inc., Norcross, Georgia, USA). About 30 mg of tissue was finely grinded in liquid nitrogen and used with the kit following manufacturer's instruction to extract total mRNA. A DNAse digestion step was carried out after extraction to remove contaminating genomic DNA using an Ambion® TURBO™ DNase (Life Technologies, Carlsbad, CA, U.S.A.) following manufacturer´s instructions. A quantitative and qualitative assessment of the isolated RNA was performed using a NanoDrop® 2000c Spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, U.S.A.). Purity was also estimated based on the absorbance ratios of the RNA samples at 260/280 nm and 260/230 nm. For pure RNA, ratios should be of about 2.0 and 2.0-2.2, respectively (T042‐Technical Bulletin, Thermo Scientific). RNA integrity was assessed by an electrophoresis in a 2.0% agarose gel. Good quality RNA should present well defined 28S and 18S bands. RNA quality was also analysed using a RNA Nano Kit Chips from the Agilent 2100 Bioanalyzer System (Agilent Technologies, Santa Clara, CA, U.S.A.). Using this approach, a good RNA sample should present well defined peaks corresponding to 28S and 18S bands and a RNA Integrity Number (RIN) above 7. The absence of DNA in RNA treated samples was verified by standard polymerase chain reaction (PCR). RNA Sequencing was conducted by LC-Sciences (Houston, TX, USA) following the TruSeq® Stranded mRNA  Sample Preparation Guide (Illumina)