title: GSM4761298: EVI_Wild type replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: For Poly-A RNA sequencing (RNAseq) analysis of Evi1-mediated modulation of gene expression RNA was extracted from murine haematopoietic progenitor cells at 48 hrs after lentiviral transduction with Evi1-WT, Evi1-S436A or vector only and untransduced cells using the RNA/DNA Purification Micro Kit (Norgen Biotek Corp.). Libraries were prepared with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina (FWD) using an input of 200 ng and performing 14 cycles of amplification. Indexed libraries were then quantified using the Kapa Illumina Library quantification kit (Cat 07960336001) and pooled. 1 x 75bp sequence reads were generated by clustering 2.0pM of the library pool on a NextSeq500 High throughput run