title: GSM4648440: WT Endoderm Rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were collected and resuspended in Trizol (Thermo, 15596018). RNA was extracted using Qiagen RNeasy plus kits according to provided protocols. Genomic DNA was eliminated in column during RNA extraction using DNase (Qiagen, 79254). The quality of the RNA samples was tested on Agilent BioAnalyzer. Libraries were prepared using NEB reagents (NEBnext ultra directional RNA library prep kit (E7420S), NEBnext rRNA depletion kit (E6310S), NEBnext multiplex oligos fo Illumina sequencing (E7335S)). The concentrations of library cDNA samples were analyzed using Qubit. Sequencing was performed using Illumina HiSeq2000.