title: GSM1833209: E2F3A_MEF; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. E2F- ChIP DNA was processed to generate libraries using TruSeq ChIP DNA library prep kit (Illumina). Briefly, 10 ng of pull down DNA was used to ligate with barcoded adaptors, amplified with 14 cycles of PCR, and then a 200-300bp library was collected using E-gel (Invitrogen).