title: Canine fecal microbial 16s rRNA metagenome sequencing
design: Fresh fecal samples were collected from ten female dogs with hound bloodlines (6.13 ± 0.17 yr; 22 ± 2.1 kg) fed five test diets containing graded levels of a fermentable fiber, potato fiber (0.0, 1.5, 3.0, 4.5, or 6.0% as-is) in a replicated 5 x 5 Latin square design. Each dog fed an experimental diet for 14 days; 10 days of adaptation to that diet followed by 4 days of fecal collections. One fresh fecal sample was taken from each dog for microbial analysis. The objective of this study was to evaluate the effect of increasing dietary potato fiber concentrations on the fecal microbiome of healthy adult dogs.
library_construction_protocol: Fresh fecal samples were collected from ten female dogs with hound bloodlines (6.13 ± 0.17 yr; 22 ± 2.1 kg) fed five test diets containing graded levels of a fermentable fiber, potato fiber (0.0, 1.5, 3.0, 4.5, or 6.0% as-is) in a replicated 5 x 5 Latin square design. Each dog fed an experimental diet for 14 days; 10 days of adaptation to that diet followed by 4 days of fecal collections. One fresh fecal sample was taken from each dog for microbial analysis. The objective of this study was to evaluate the effect of increasing dietary Potato Pulp concentrations on the fecal microbiome of healthy adult dogs.