title: GSM3891199: RNA-seq, wild-type 3; Mus musculus; RNA-Seq
library_construction_protocol: Livers perfused with phosphate buffered saline (10-30 mg pieces) were added to 1 ml of TRIzol (Life Technologies), mechanically homogenized and RNA was extracted according to the manufacturer's instructions. RNA concentration was determined with a NanoDrop®ND-1000 spectrophotometer and RNA quality was assessed with the Fragment Analyzer (Advanced Analytical Technologies, Ankeny, USA). Libraries were prepared with the TruSeq Stranded Total RNA Ribo-Zero Globin kit according to the manufacturer's instructions. Sequencing libraries were loaded onto one lane of a HiSeq 2000 flow cell and sequenced at 100 cycles.