title: GSM4384838: In vivo-icSHAPE-HEK293T-DMSO rep2; Homo sapiens; OTHER
library_construction_protocol: Total RNA was extracted using RNeasy Mini Kit (Qiagen) and digested with DNaseI to remove DNA contamination. Purified RNA was ribo-depleted and used for further library construction. icSHAPE library preparation protocol was followed (PMID:26766114). Briefly, ribo-depleted RNA was biotin-clicked, fragmented and end-repaired. Next, RNA was 3' end ligated followed by cDNA synthesis and circularization. Final library was PCR amplified.