title: Transcriptome of hexaploid hulless oat in response to salinity stress
design: Total RNA from the control and NaCl-treated samples was extracted with an RNeasy Plant Mini kit (Qiagen) following the manufacturer’s instructions. After digestion with RNase-free DNase I (Promega) to eliminate DNA, Poly (A) mRNA was isolated from total RNA using an Oligotex mRNA mini kit (Qiagen). The mRNA samples purified from salt stressed samples taken at different time points were pooled equally and used for double-stranded cDNA synthesis using a Just cDNA Double-Stranded cDNA Synthesis Kit (Agilent Technologies); the same amount of mRNA from unstressed plants was used for the control. The quality of total RNA, mRNA, and final double-stranded cDNA samples was verified with a NanoDrop (Thermo Scientific, Waltham, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies). Approximately 5 μg of each cDNA sample was sheared via nebulization into small fragments and sequenced using a Roche 454-GS FLX Titanium System.