title: RNA-seq of BT-549
design: Total RNA was isolated from BT-549 cells and purified by using RNeasy mini kit (Qiagen, Germany). The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. The construction of cDNA library with Tru-seq mRNA sample preparation kit (Illumina, USA) was sequenced in Illumina platform.