title: GSM4762251: LR-V_EL38_C; Vitis vinifera; RNA-Seq
library_construction_protocol: For each biological replicate, 600 berries were randomly separated from both sunny and shade sides of at least 100 clusters within 15 vines. The sampling time  was fixed at 10:00 to 11:00 am, and three biological replicates were collected with the same method at each sampling date. After being washed with distilled water, a sub-sample of 100 berries from each biological replicate was subjected to the physiological measurements, including berry fresh weight, total soluble solids (TSS) content and titratable acidity (TA), the rest were frozen in liquid nitrogen immediately and transported to the lab in dry ice for the subsequent metabolites determination and transcriptional analysis. cDNA libraries were prepared for sequencing using standard Illumina protocols.