title: GSM1177466: EXP_12_Dp5; [Clostridium] symbiosum; Bacteroides thetaiotaomicron VPI-5482; Bacteroides ovatus ATCC 8483; Bacteroides caccae ATCC 43185; Collinsella aerofaciens ATCC 25986; Marvinbryantia formatexigens DSM 14469; Escherichia coli str. K-12 substr. MG1655; [Eubacterium] rectale ATCC 33656; Desulfovibrio piger GOR1; RNA-Seq
library_construction_protocol: Each frozen sample was suspended in a solution containing 500 mL of acid-washed glass beads (Sigma-Aldrich), 500 mL of extraction buffer A (200 mM NaCl, 20 mM EDTA), 210 mL of 20% SDS, and 500 mL of a mixture of phenol:chloroform:isoamyl alcohol (125:24:1, pH 4.5; Ambion), and then lysed by using a bead beater (BioSpec Products; maximal setting; 4 min at room temperature). Cellular debris was removed by centrifugation (8,000 × g; 3 min at 4°C). The extraction was repeated, and nucleic acids were precipitated with isopropanol and sodium acetate (pH 5.5). DNA libraries were prepared for sequencing using a modified version of Illumina's sample preparation protocol (see  PMID:22030749 for protocol details)