title: GSM3267087: WT_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized and livers harvested after perfusion with cold 1XPBS to reduce blood contaminants. Livers were dissected in a petri dish with 1XPBS on ice, and a portion was snap-frozen in liquid nitrogen for extraction of mRNA. Total liver RNA extractions were performed using Trizol (Thermo) extraction. Contaminant DNA was removed by RNase free-DNase (Thermo). RNA-seq libraries were prepared with TruSeq Stranded mRNA Library Prep Kit (Illumina), according to manufacturer's instructions.