title: NextSeq 500 paired end sequencing; Single-nucleotide resolution mapping of DNA abasic sites by snAP-seq
design: Single-nucleotide resolution mapping of DNA abasic sites by snAP-seq
library_construction_protocol: Synthetic DNA was generated by primer extension using template oligos (see methods section for sequences) Input libraries were prepared for each enriched (snAP-seq) library. DNA was end-prepped using an NEBNext Ultra II kit, followed by adapter ligation using Truseq LT indexed adapters (Illumina). PCR amplification conditions were kept consistent with corresponding enriched (snAP-seq) libraries.