title: GSM2189101: 1-0-1-1-BTN35-C39-1782070112-30ul-1-IL5413-N703-N508 BTN35 Mic-scRNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: Acute brain slices were cut from 5-7 weeks old male mouse and after papain treatment dissociated mechanically (SI Methods). Live cells were purified by either magnetic bead-activated cell sorting (MACS;Miltenyi Biotec) or by fluorescence-activated cell sorting (FACS). For cell type specific isolation genetically labeled MSN subtypes D1-, D2-MSN, and astrocytes were purified by FACS. Single cells were captured on a microfluidic chip on the C1 system (Fluidigm) and whole-transcriptome amplified cDNA was prepared on chip using theSMARTer Ultra LowRNAkit for Illumina (Clontech). For smart-seq2 protocol, three MSN populations (D1-MSN tdTom+, D1-MSN GFP+ and tdTom+/GFP+ MSNs) sorted individually into 96 well plates with lysis buffer and spun down and frozen at -80°C and amplified using the protocol as described previously (Picelli et al., 2013). Libraries were prepared using Illumina Nextera XT kit per illumina's protocols.