title: GSM2028170: A1 Input; Homo sapiens; RIP-Seq
library_construction_protocol: RNA-protein cpmplexes were crossslinked by irradiating cells with UV (254 nm) and Flag-SIRT7-RNA complexes were isolated with anti-Flag antibody from nuclear lysates. RNA libraries were prepared for sequencing using standard Illumina protocols, Briefly, the CLIP-seq experiments were performed in biological duplicates. Co-immunoprecipitated RNA from anti-Flag-SIRT7 and IgG control immunoprecipitations was purified with TRIZOL, and treated with DNase I. The RNA-seq libraries were created using the NEBNext Ultra RNA Library Prep kit for Illumina (E7530) with NEBNext Multiplex Oligos for Illumina (E7300). 50 cycles of sequencing were performed on the Illumina HiSeq 2000 Instrument. cDNA libraries from total RNA were used as Input.