title: GSM4586712: R2795; Aspergillus niger; RNA-Seq
library_construction_protocol: RNA was isolated from mycelial biomass samples obtained from batch cultivated A. niger strains N402 and the ΔkexB strain, using TRIzol (Invitrogen). RNA was purified afterwards with NucleoSpin RNA Clean-up kit (Macherey-Nagel) with DNase treatment. Concentration and quality of the RNA was determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific) and by gel-electrophoresis, respectively RNA libraries were prepared for sequencing using standard Illumina protocols