title: GSM2794702: Cooling_RBM3 knockdown_case; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extract by using trace rna sample extraction kit. Total 100ng RNA for the construction of sequencing libraries mRNA were enriched by Oligo(dT) bread and then fragmented by fragmentation buffer. cDNA was synthesized by reverse transcription using 6bp random primers and purified by QIAQuick PCR kit. After adapter ligation, DNA was PCR amplified sequences and band isolated from an agarose gel.