title: GSM2026331: 14.5 + 2 + JTE + CTGF 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared using  the Rneasy Kit and on column genomic digestion (Qiagen) Total RNA with an integrity number of ≥ 8 was used. mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module. After chemical fragmentation by incubating for 15 min at 94°C the sample was directly subjected to the workflow for strand specific RNA-Seq library preparation (Ultra Directional RNA Library Prep, NEB)