title: GSM4761014: ZJL_miRNA; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was extracted from renal tissues using the Trizol LS reagent . After total RNA quality check, the rRNA was removed using the Ribo-ZeroTM rRNA removal kit and purification and fragmentation of RNA was performed at the same time. First-strand cDNA was synthesized via reverse transcription, followed by second-strand cDNA synthesis. After terminal repair and purification, the cDNA library was amplified through PCR. Finally, samples were sequenced using a 2×150 base paired-end configuration with the Illumina Hiseq 2500 (Illumina, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols