title: GSM1832317: Gfi1b Wild-Type ROSA-Cre; Mus musculus; RNA-Seq
library_construction_protocol: Lin- cKit+ CD41+ CD61+ Megakaryocytes were sorted from mice tibiae, femora and humeri using a MoFlo cell sorter and RNA was extracted using a MagMax-96 Total RNA Isolation kit (Ambion). RNA-Seq, libraries were prepared from RNA extracts using the TruSeq Stranded mRNA kit from Illumina according to the manufacturer’s instructions and sequenced using using the TruSeq PE Clusterkit v3-cBot-HS.