title: RNA-Seq of wheat seedlings shoot
design: RNA isolation was carried out for two biological replicate of each treatment. Hence, 14 separate libraries consisting of two biological replicates of seven treatment were prepared with Illumina-compatible NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs, MA, USA) according to the manufacturer's protocol and paired end sequencing was done using Illumina (HiseqTM 2500) sequencing technology to obtain reads of 150 bp length.