title: GSM2443167: variant F; Paenibacillus polymyxa; RNA-Seq
library_construction_protocol: Total RNAs from ‘B’ and ‘F’ type cells of P. polymyxa E681 were isolated from 2-day-old cultures grown in TSA using the RNeasy mini kit with on-column DNase I treatment (Qiagen Inc., Hilden, Germany). After DNase I treatment, a MICROBExpress bacterial mRNA enrichment kit (Ambion, Carlsbad, CA) was used to remove bacterial rRNA from the total RNA sample. RNA-sequencing libraries were prepared using an Illumina TruSeqTM RNA sample prep kit (Illumina, San Diego, CA), according to the manufacturer’s protocols. Sequencing was performed on an Illumina HiSeq2000 instrument using the reagents provided in the Illumina TruSeq PE Cluster kits (Illumina).