title: DNA-Seq of Cardiac Biopsy in Pediatric Stem Cell Transplant
design: "Briefly, a 0.5mm formalin-fixed paraffin-embedded block underwent deparaffinization and lysis with xylene, ethanol, proteinase K, and RNase, followed by column-based DNA extraction using the QIAamp FFPE extraction kit.  DNA then underwent enzymatic repair of formalin-related damage, endonuclease restriction of the 3' tail, ligation of sequencer-specific adaptors and barcodes, and magnetic bead purification with 250-400bp size selection (New England Biolabs), resulting in 18.2 ng of DNA."