title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of antennal tissue of male and female honey bees (Apis mellifera) at 2 different time points of the day
design: RNA-seq of antennal tissue of male and female honey bees (Apis mellifera) at 2 different time points of the day
library_construction_protocol: Drones (males), morning- and afternoon trained foragers (females) were collected at 2 different time points. Collected bees were flash frozen in liquid nitrogen and antennae were dissected on dry ice.  Total RNA was extracted from the pool of antennae (10 antennae per sample) using Trizol reagent.  Truseq mRNA stranded sample preparation guide was followed for library construction.