title: GSM2255510: Wild-type Nutlin-3 replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol/chloroform-extracted total RNA was further purified with the RNeasy Mini Kit (Qiagen) incorporating a DNaseI step (Qiagen) to remove DNA contamination. Libraries were prepared from rRNA-depleted total RNA isolates and according to standard Illumina guidelines using in-house adapters (Lamble et al., 2013)