title: GSM4648394: SA29499_IRI 4; Mus musculus; RNA-Seq
library_construction_protocol: Renal mRNA profiles of 8-week-old C57BL/6J mice challenged with ischemia-reperfusion (24 hours), cisplatin (72 hours), and rhabdomyolysis (24 hours) were generated by RNAseq analysis. Total RNA was isolated from harvested renal cortical tissues using the RNeasy Plus Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacturer's protocols. Total RNA samples used for library construction and sequencing (Quick Biology, Pasadena, CA). RNA integrity, quality and purity were analyzed by Agilent 2100 Bioanalyzer. Libraries for RNA-Seq were prepared with KAPA Stranded mRNA-Seq Kit (KAPA Biosystems, Wilmington, MA) according to the manufacturer's protocols. The workflow consisted of mRNA enrichment using bead capture for poly-A selection, cDNA generation, end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification of library fragments. Final Library size distribution was determined by using an Agilent 2100 Bioanalyzer using the High-Sensitivity DNA Kit and its quantity was analyzed by Life Technologies Qubit 3.0 Fluorometer. Libraries were pooled and sequenced on the Illumina HiSeq 4000 platform to obtain 150-bp paired-end reads, 20 million reads (10 million reads pairs) per sample.