title: GSM3656430: shS6K1 cells with sh5: TRNC0000003162, replicate 2 [shS6K1.sh5.2]; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in Tri-Reagent (Sigma-Aldrich, #T9424-100ml) and RNA was extracted using the Direct-zol MiniPrep Kit (Zymo Research, #R2050) with in column DNAse I treatment. Total RNA (200ng) was used to prepare 3' QuantSeq mRNASeq libraries (Lexogen, #KO152x96)