title: GSM2966852: 5C_Lucio5C-D1-HB-20-30-Combined; Homo sapiens; OTHER
library_construction_protocol: 3 ml of 16% formaldehyde solution was added to the 50  ml tube to obtain a  final  concentration of 1%. The content was quickly mixed by inverting the tube twice and left at RT for 10 min for cross-linking.  2.5  ml  of  2.5  M  glycine  was  then  added  to  the  tube to  stop  cross-linking.  The content was quickly mixed by inverting the tube twice and left at RT for 5 min, and then on ice for at least 15 min.  Subsequently, cells were collected by centrifugation (2000 rpm, 10 min), and the cell pellet was frozen on dry ice and then stored at -80°C. 5C was performed as in Maria A. Ferraiuolo et. al, 2012, http://www.sciencedirect.com/science/article/pii/S1046202312002769?via%3Dihub