title: Multiplexed SSU rDNA V4 amplicons from Scotland fish farms sediment samples
design: PCR products of the SSU rDNA V4 region containing tag sequences at their 5' and 3' ends as sample labels were quantified and pooled in equimolar quantities. Based on the pool was prepared one sequencing library using the Illumina TruSeq Nano DNA LT Sample Prep kit. The library was sequenced on a MiSeq instrument for 502 cycles (251 cycles paired-end) using the MiSeq Reagent Nano Kit v2.