title: Characteristics of SRB community in Sediments from the East China Sea by high-throughput sequencing
design: Sulfate-reducing bacteria (SRB), which obtain energy from dissimilatory sulfate reduction, play a vital role in the carbon and sulfur cycles. The dissimilatory sulfite reductase (Dsr), catalyzing the last step in the sulfate reduction pathway, has been found in all known SRB that have been test so far. Using the dissimilatory sulfite reductase beta subunit gene (dsrB), we aimed to assess the advantages and disadvantages of amplicon-based high-throughput sequencing (pyrosequencing and Illumina HiSeq2500 sequencing) and traditional clone library of SRB communities in sediments from the East China Sea. 