title: GSM3043784: Clarke_Bulk-Stim_Donor-33_N_CD103neg; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq was performed as described previously by Seumois et al 2016 and Ganesan et al 2017 using smart-seq2 method  with 0.5ng of total RNA. Total RNA was purified using a miRNAeasy kit (Qiagen) and quantified as described previously (Seumois et al.Am J Clin Exp Immunol. 2012; PMID:23304658). Barcoded Illumina sequencing libraries were sonstructed as per the protocol described by illumina (Nextera XT library preparation kit, Illumina). DNA sequencing - Libraries were sequenced on the HiSeq2500 Illumina platform to obtain 50-bp single end reads (barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina)).