title: Illumina HiSeq 2500 paired end sequencing; The role of cytokinins in clubroot disease
design: The role of cytokinins in clubroot disease
library_construction_protocol: Total RNA was extracted using Trizol according to the manufacturer's protocol Hypocotyls or the upper 1 cm of root was collected from 25 plants per biological replicate Plants were grown at 100 umol m-2 s-1 irradiance, 9 h photoperiod, day time temperature of 20oC and a night time temperature of 18 oC Plants were infected with 2E6 spores of P. brassicae 14 days after germination RNA library preparation