title: 16S rRNA gene amplicon sequencing of microbiome in rumen liquid: Sample MTH-55
design: 16S rRNA amplicon sequencing of rumen microbiome. A total number of 10 multiparous dairy cows were involved in our study over the transition period, which was defined by three weeks before and after parturition. Rumen liquid sample was taken over seven continuous weeks. As a consequence, seventy rumen samples were collected and used for DNA extraction.  Amplicons for next generation sequencing were prepared by using bacteria universal primer (515F and 806R) to amplify the V4 region of bacterial 16S rRNA. Equal proportion of PCR product from each sample was pooled and analyzed by Illumina MiSeq platform. With the sequence information, we aim to evaluate the stability of the rumen microbiome in dairy cows over the transition period.