title: GSM4699145: CTCF-ChIP-seq-INPUT-Mut-rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq: Lymphoblastoid cells (1x10^7 cells) were ﬁxed in phosphate-buffered saline (PBS) with 0.96% formaldehyde for 8 minutes at room temperature. Crosslinking was quenched with 125 mM Glycine. The cells were washed with PBS and precipitated via centrifugation. The cell pellet was stored in -80°C until use. ChIP-seq: Libraries were prepared using Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences, catalog no. 21024) according to manufacturer's protocol. 4C-seq: 4C-seq was performed and ibraries were prepared according to standard procedures (Krijger et al. Methods 170, 17–32 (2020))