title: GSM3267425: Mouse LGN P20 control rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized on E18, P4, P10, P16, P20, P27 or P32. Each time point includes data from four mice total, processed as four independent samples.  After isoflurane anesthetization, mice were decapitated and the brain was isolated. Mouse brains were dissected and 300 um coronal sections were made on a Leica VT1000S vibratome. The dorsal LGNs were then microdissected in ice cold PBS after visual identification using a Nikon SMZ-10A brightfield dissection microscope, and flash frozen. Total RNA was trizol-extracted and purified using the Qiagen RNeasy Micro kit. After RNA extraction, total RNA was depleted of ribosomal RNA, heat-fragmented to ~350 bp using the NEBNext kit, and processed with adapters to allow nonspecific sequencing on an Illumina NextSeq 500 System.