title: 16S rRNA seq of Melanogrammus aeglefinus microbiome: Stomach sample
design: Stomach content and mucus lining was homogenized and DNA extracted                 with QIAamp DNA Stool Mini Kit from Qiagen. Primers U786F and A1067R covering the                 V5/V6 variable region of the 16S rRNA were used. Amplicon fusion primers were                 designed for 454 sequencing (Roche). Following PCR amplification and verification on                 agarose gels, the PCR products were purified with the Agencourt AMPure XP PCR                 purification system to eliminate primer dimers. DNA concentrations were measured and                 PCR products pooled for sequencing.