title: GSM4697996: Control_rep2; Sus scrofa; miRNA-Seq
library_construction_protocol: PBMCs were separated from the whole blood by a density gradient centrifugation method; Total RNA was extracted using mirVana miRNA isolation kit (Thermo Fisher); miRNA library was constrcted using NEBNext Multiplex Small RNA Library Prep kit (cat. # E7560S) with 500 ng total RNA as input. miRNA libraries were prepared for sequencing using modified NEB protocol.