title: GSM1174183: Sporocyst; Schistosoma mansoni; RNA-Seq
library_construction_protocol: Total RNA from 10,000 parasites using Trizol (invitrogen) and DNase treatment (DNA-free RNA Kit, Zymo Research, Irvine, CA). Libraries were constucted using the TruSeq RNAseq Sample Prep kit. The libraries were quantitated by qPCR, pooled and sequenced on one lane for 100 cycles on an Illumina HiSeq 2000 using a TruSeq SBS sequencing kit version 3.