title: GSM4760180: OCI-AML3_sgIRF8_2_Rep1.txt; Homo sapiens; RNA-Seq
library_construction_protocol: TRIzol was used to extract total RNA according manufacture's protocol. RNA-seq library was contructed using Illumina TruSeq sample prep kit V2.Briefly, polyA RNA was selected and fragmented enzymatically. First strand of cDNA was synthesized using Super Script II reverse transcriptase, and then second strand was synthesized. Double-stranded cDNA was end-repaired, 3'-adenylated, ligated with indexed adaptor, and then PCR-amplified. Briefly, polyA RNA was selected and fragmented enzymatically. First strand of cDNA was synthesized using Super Script II reverse transcriptase, and then second strand was synthesized. Double-stranded cDNA was end-repaired, 3'-adenylated, ligated with indexed adaptor, and then PCR-amplified.