title: GSM2188250: Kidney, normal, pool#1 (n=3 donors)_total RNA; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from 1 ug of each tissue using TriReagent followed by RNA cleanup using QIAGEN RNeasy Mini Kit with DNase I. Equal amount of RNA from 3 tissue samples (or 5 tissue samples for normal intestine/colon) were pooled and External RNA Controls Consortium (ERCC) standards RNA Spike-In Mix 1 (ThermoFisher Scientific) were spiked into pooled RNA samples. 1 ug total RNA from each pool was used to prepare sequencing librairies with Illumina TruSeq RNA Sample Prep Kit RNA libraries were prepared for sequencing using standard Illumina protocols