title: Metagenomic shotgun sequencing of ocean water from Patagonia (virome)
design: We filtered 40 L of 5-meter deep ocean water from Northern Patagonia using Sterivex PES 0.22 um filters. The eluate was concentrated down to 25 mL using a tangential flow concentrator of 100 Kda. We then extracted DNA from the concentrate using Silica spin columns. DNA extracts were sequenced in an Illumina MiSeq machine. Libraries were prepared using Illumina TruSeq