title: GSM2256351: mKO2lo-IL9-2-3; Mus musculus; RNA-Seq
library_construction_protocol: Cells were directly sorted into the lysis buffer and follow the modified the protocol initially developed for single-cell RNA-seq (Tang, 2011) to accommodate 100-200 sorted cells by doubling reaction volumes with extra buffers until the step for second strand DNA synthesis RNA libraries were prepared for sequencing using standard Illumina protocols