title: GSM1467664: RRBS_Liver_p53_KO_3; Mus musculus; Bisulfite-Seq
library_construction_protocol: p53 heterozygous male and female mice were bred to produce p53 wild type (WT) and p53-null offspring. Mouse tail genotyping was performed by PCR. Animals were sacrificed at the age of 16 weeks and liver samples were collected. Reduced representation bisulfite sequencing (RRBS) was performed according to a previously published protocol  (Boyle, P. et al. 2012). Briefly, genomic DNA was isolated using the PureLink genomic DNA kit (Invitrogen) according to the manufacturer’s instructions and subjected to enzymatic digestion by MSPI (New England Biolabs) for 16 hours. Klenow polymerase (New England Biolabs) was then added to each sample for end filling and poly-A tailing, followed by Truseq adapter ligation (Illumina). The library was then pooled and subjected to either bisulfite treatment as in the RRBS protocol or to an OX-RRBS protocol using the CEGX kit (Cambridge Epigentix). Validation of oxidation efficiency was done with the provided DNA sequences that were also A tailed and adapter ligated.