title: GSM2188003: 3T3-L1, CRTC2-ChIP, Forskolin, Rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were harvested in Isol-RNA lysis reagent (5 PRIME) and purified according to the manufacturer's instructions. Purified RNA was subjected to standard mRNA sample preparation for sequencing and sequenced on the Illumina platform according to the manufacturer’s instructions. ChIP-seq libraries of CRTC2 enriched DNA were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).