title: GSM3204794: Th17-IL-10+ Day 5 2h RNA-seq Repl_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from at least 106 cells using the E.Z.N.A.® Total RNA Kit I (Omega Bio-tek), including on-column DNAse treatment, according to manufacturer's instructions. 250 ng for each sample of total RNA isolated from T cell clone pools with the E.Z.N.A.® Total RNA kit I (Omega Bio-tek) was submitted for high coverage (50 million paired-end reads), Tru-Seq standard, strand-specific RNA-sequencing.