title: whole-genome re-sequencing of a Plasmodium vivax clinical isolate  in China-Myanmar border area
design: Genomic DNA for P. vivax CMB-1 sequencing was extracted from the whole blood of a patient with microscopically positive for P. vivax and PCR confirmed for single P. vivax infection in China-Myanmar border area. Here we selected the sample with highest parasite density (3.8% of parasitemia) to ensure the integrity of sequencing. Genomic DNA was extracted using the QIAGEN DNeasy Blood & Tissue Kit, and sheared into 500 bp fragments using a Covaris S2 instrument and used the fragmented DNA molecules to construct the Illumina sequencing libraries.