title: D.koepferae-D. buzzatii F1 hybrid testes small RNA population
design: Small RNA was purified from 333 pairs of D. koepferae-D. buzzatii F1 hybrid testes, following the manual small RNA purifying protocol described by Grentzinger et al. (2013), which significantly reduces endogenous contamination and degradation products abundance. After small RNA isolation, samples were gel-purified and precipitated. Sequencing was performed on an Illumina Hiseq 2500 platform by FASTERIS SA (Switzerland).