title: GSM4760264: Linehan-20; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cell lines or cryo-pulverized tumor/normal tissues using Trizol Reagent (Invitrogen) following the manufacturer's protocol and stored at -80 °C. RNA concentrations and purities were measured using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific) and 5μg of total RNA was sent to ACGT, Inc. (Germantown, MD, USA) for RNAseq analysis. All samples were processed with the TruSeq mRNA kit using oligo-dT beads to capture polyA tails, sequenced on a single flowcell of an Illumina HiSeq 2500 instrument, and data were delivered as a Multiplex Fast-Q file with adaptors trimmed.