title: GSM2254908: spermatocytes; Mus musculus; ChIP-Seq
library_construction_protocol: Germ cells were separated according  y (Bellve, 1979). Briefly,  8  twelve weeks old c57Bl/6J male mice were  perfused, and testes were dissected. Testes were digested with collagenase, trypsine  and DNase. The cells were loaded into 2-4% gradient of BSA and allowed to sedimentate. The spermatocytes and spermatids fractions were collected. The purity was confirmed by staining with DAPI of the small fractions of cells. Cells were fixed with paraformaldehyde and used for ChIP-seq. The libraries were prepared from 50 ng of ChiP and input material using the NEBNext Ultra DNA library Prep Kit for Illumina (NEB #E7370S). massively parallel 50-bp sequencing in single end mode for ChIP-seq