title: GSM1831735: HS5-1 Inversion rep2; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted from cultured cells (inversion and wide type control in duplicates) using an RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol. Messenger RNA was isolated from the total RNA by oligo (dT) magnetic beads, and fragmented under heating condition. After the first and second cDNA strand synthesis, as well as ends repairing, the 3’ ends of cDNA fragments were added with a single ‘A’ nucleotide to facilitate adapter ligation. The libraries were then generated by PCR amplification of the cDNA products, and were sequenced on the Illumina sequencing platform of HiSeq 2000.