title: GSM2026539: healthy_iPSC_50days_neuron; Homo sapiens; RNA-Seq
library_construction_protocol: Cell samples of HDF2-iPS and SC128 were collected at four stages during the neuronal differentiation，i.e., iPSC (day0) , IA (day6), NE (day 18), mature neuron (day50). RNA was extracted with TRIzol Reagent(Ambion) according to the manufacturer’s protocol. We constructed RNA-seq libraries following the protocol described in Illumina TruSeqTM RNA Sample Preparation Guide. Briefly, we started with 400 ng of total RNA. We then purified poly-A containing mRNA molecules by poly-T oligo-attached magnetic beads. After RT and complementary cDNA strand synthesis, we did end-repair and adapter ligation. Finally we amplified the cDNA by 15-cycle PCR. The concentration of the amplified product was determined by Qubit Fluorometer (Invitrogen).