title: Pagrus_major.5
design: The complete isolated gDNA were quantified using NanoDrop spectrophotometerand a Qubit fluorometer. Total gDNA are subjected to other steps i.e. fragmentation withCovaris G-Tube to obtain &gt; 20 KB fragments, Small fragments filtering with 0.45XAMPure  , DNA fragment ends repair with ExoVII treatment, ligate the Blunt adapters withdouble standard DNA fragments, attach the primer and polymerase to the SMRTbell TMtemplates (Template Prep Kit 1.0 (PN 100-259-100)) and addition of MagBeads.