title: GSM2611691: Saline P21 male litter 3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Subjects were deeply anaesthetized with isofluorane, decapitated, and brains were frozen on dry ice and stored at -80˚C. On a microtome, punches (~1uM3) were be collected of the BLA, as well as the prefrontal cortex, nucleus accumbens, bed nucleus of the stria terminalis, and the hippocampus. Transcriptomics was assessed in male amygdala micropunches from P10 (n = 4 saline, 4 vpa) and P21 (n = 7 saline, 6 vpa). RNA sequencing libraries were prepared by the Yerkes Nonhuman Primate Genomics Core (NHPGC). Total RNA was prepared using the QIAGEN RNEasy Micro Kit. Libraries were generated from 5 ng of Total RNA using the CLONTECH SMARTer HV kit, and barcoding and sequencing primers were added using NexteraXT DNA kit. Libraries were validated by microelectrophoresis, quantified, pooled and clustered on Illumina TruSeq v3 flowcell. The clustered flowcell was sequenced on an Illumina HiSeq 1000 in 100-base single-read reactions. RNA libraries were prepared for sequencing using standard Illumina protocols