title: GSM1468330: ESC.WT.b1 [RNA-Seq]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was purified from cells with TRIzol (Invitrogen) PolyA+ RNA was isolated using Dynabeads Oligo(dT)25 (Invitrogen) beads and converted into dsDNA using the dUTP method (Parkhomchuk et al., 2009). DNA was end-repaired using End-It Repair Kit (Epicenter), tailed with an A using Klenow exo minus (NEB M0212), and ligated to custom adapters. Fragments of 300±100 bp were size-selected and subjected to ligation-mediated PCR amplification (LM-PCR) using Q5 polymerase (NEB M0530) to generate strand-specific libraries.