title: GSM1467830: Lm_OlderAdult1_6hr; Homo sapiens; RNA-Seq
library_construction_protocol: Human monocyte RNA was purified from monocytes after using TRIzol reagent (Invitrogen) and RNeasy Mini Kit (Qiagen) and eluted into 30 uL RNase-free water.  Libraries were prepared using 120 ng RNA input. Illumina's TruSeq v2 library kit was used for library construction. All libraries were constructed per manufacturer's guidlines with the exception of generating strand-specific libraries according to the “deoxyuridine triphosphate (dUTP)” method (Levin et al., 2010).  An Illumina HiSeq 2000 (Illumina, San Diego, CA) was used for sequencing, with a single-end-sequencing length of 50 nt.