title: GSM1466346: ChIPSeq-ABI5_mock_2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: After nuclei isolation, chromatin was sonicated to 100-400 bp fragments. Native ABI5 in wild-type Col-0 plants was immunoprecipitated by a rabbit polyclonal ABI5 antibody (cat #ab98831 lot #GR24437-1, Abcam, USA). Tagged ABI5 proteins in the transgenic lines were immunoprecipitated by a rabbit polyclonal GFP antibody (cat #A11122 lot #1232939, Life Technologies, USA). Col-0 was immunoprecipitated with either rabbit IgG or the GFP antibody as mock IP for the native ABI5 and tagged ABI5, respectively. Libraries were prepared according to Illumina's instructions accompanying TruSeq DNA sample prep kit and sequenced by Illumina HiSeq 2500 according to the manufacturer's instructions.