title: GSM2255747: Con2_WSTF.bam; Mus musculus; ChIP-Seq
library_construction_protocol: For each ChIP-sequencing (ChIP-seq) replicate, bilateral 14 Ga NAc punches were pooled from five to 10 mice. Tissue was lightly fixed to cross-link DNA with associated proteins and the material was further sheared using a Bioruptor to obtain mostly 100–300 bp fragments and immunoprecipitated. Immunoprecipitated DNA were prepared for ChIP-seq using an Illumina kit according to the manufacturer’s instructions. Each experimental condition was analyzed with independent biological triplicates. Amplification and size selection were confirmed with a BioAnalyzer. The resulting libraries were sequenced on an Illumina HiSeq 2500 with 100 bp read length.