title: GSM4586748: ΔhbtRC+pVSV105::lacI-hbtRC_1; Aliivibrio fischeri; RNA-Seq
library_construction_protocol: Cells were lysed with RNAprotect Bacterial Reagent (Qiagen). RNA was extracted with RNeasy Mini Kit (Qiagen). DNA was degraded with TURBO Dnase (Invitrogen). Ribosomal reduction (RiboZero) and strand-specific library preparation (TruSeq) were performed at the University of Minnesota Genomics Center per standard Illumina protocols. Paired-end 50-bp sequence analysis was performed on an Illumina HiSeq 2500 on high-output mode.