title: GSM2616052: Mut169dupA replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: TRI-reagent Indexed directional cDNA libraries were prepared using TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) according to manufacturer's instructions. Adapter-ligated cDNA libraries with an insert size of 200-250 bp were size selected on 1.5% agarose gel cassettes using Pippin Prep instrument (Sage Science, Beverly, MA, USA).