title: GSM1649988: P60 Female F1i sample 6; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from tissues of interest using the Trizol reagent (Life Technologies) according   to the manufacturer instructions and further purified using DNase I digestion and the RNeasy kit  (Qiagen). 3ug of  total RNA were used for preparing libraries according to the Illumina Tru-Seq RNA sample preparation   protocol. Average fragment size of 250 bp was selected Each RNA-seq library was sequenced on an individual HiSeq200 lane generating 59bp single-end reads