title: GSM2612354: CDA-KLF1 ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP was performed as described (Hodge et al. A global role for EKLF in definitive and primitive erythropoiesis. Blood 2006). Cell lines were induced with 4-OHT (or ethanol control) and incubated for 3 hours before fixation with 0.4% formaldehyde. Chromatin was fragmented to approximately 200-400bp by sonication (Diagenode Bioruptor® for 15 minutes cycling between 30 seconds ‘ON’ and 30 seconds ‘OFF’), pre-cleared and immunoprecipitated with protein G sepharose beads (GE Healthcare). Sequencing libraries were generated from 3 pooled replicates using the Ion Xpress™ Plus gDNA Fragment Library Preparation Kit following the Ion ChIP-Seq Library Preparation protocol.