title: GSM2526094: S2 T0 RNA-seq rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: [RNA-seq] 10^7 Drosophila S2 cells were harvested on ice, washed in cold PBS and their RNA was extracted using the RNeasy Kit (Qiagen) with on column DNAse digestion according to the manufacturer's protocol. Ribosomal RNA was depleted from the total RNA (5ug) by using the RiboZero gold magnetic kit (Epicentre/Illumina) according to manufacturer's instructions. cDNA was generated with the TruSeq non–stranded kit (Illumina) according to manufacturer's instructions