title: Pooled sample consisting of samples: Cyst 708 S1|Cyst 717 S1|Cyst 719 S1|Cyst 724 S1|Cyst 726 S1|Cyst 730 S1|Cyst 733 S1|Cyst 737 S1|Cyst 743 S1|Cyst 746 S1|Cyst 773 S1|Cyst 791 S1
design: Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument