title: GSM2027579: Derivative_3-3-leaf; Zea mays; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen) and was subjected to the Illumina RNA-seq protocol, followed by sequencing using the HiSeq2000 platform. Seedling and young leaf tissues harvested from plant materials with and without Derivative 3-3 were used for RNA-seq analysis. Extracted nuclei were digested with micrococcal nuclease (Sigma-Al drich) for ChIP experiments using the maize CENH3 antibody.The ChIP-enriched DNA of Derivative 3-3 was treated with sodium bisulfite and then performed Illumina sequence. The maize CENH3 is a rabbit polyclonal antiserum and was raised against the peptides: C-RPGTVALREIRKYQKS; STSATPERAAGTGGR-C. Keyhole limpet hemocyanin (KLH)-conjugated peptide was used to immunize rabbits. RNA libraries were prepared according to standared Illumina's instructions. ChIP libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).180-400 bp fragments (contained the joint) were selected by gel extraction.