title: GSM1833614: H3K4me2-ChIPSeq_GHMTd7; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody anti-H3K4me2. Libraries were prepared using Nugen Ovation Ultralow System Cat No  0344.  Briefly, DNA was end-repaired, A-tailed, adapter ligated and amplified with 10 cycles of PCR.  DNA libraries were purified using Agencourt XP Beads, quantified with the Qubit, run on a bioanalzyer tape station and clustered to an Illumina HiSEQ 2500 High Throughput Flow Cell.