title: GSM4648562: sh_CBFB; Homo sapiens; RNA-Seq
library_construction_protocol: For transcriptome sequencing analysis, total RNA was extracted from HCT116 cells stably expressing or inhibiting CBFβ (CBFβ-LV/shRNA-CBFβ-LV groups) or their control cells (Control-LV/shRNA-Control-LV groups) using TRIzol (Life Technologies). All of the procedures were carried out by Novogene (Beijing, China) according to Illumina's protocols. RNA was subjected to fragmentation and library construction and then sequenced with the HiSeq 2000 platform. RNA libraries were prepared for sequencing using standard Illumina protocols