title: GSM4459927: 06A; Mus musculus; RNA-Seq
library_construction_protocol: Mice were killed with an overdose of isoflurane. The brains were rapidly extracted from the skull and immediately submerged in ice-cold ACSF. The brains were then blotted to remove excess liquid and the hemispheres separated along the sagittal midline. The left hemisphere was subsequently embedded in OCT and frozen in isopentane on dry ice. The hemisphere was cut into 10 μm sections on a cryostat. mRNA captured by the surface probes was reverse-transcribed using Superscript III. Next, 2nd strand synthesis, cDNA purification, in vitro transcription, aRNA purification, adapter ligation, post-ligation purification, a second 2nd strand synthesis and purification were carried out using an automated MBS 8000 system as described previously (Jemt et al). cDNA was amplified by PCR using Illumina Indexing primers and purified using carboxylic acid beads.