title: GSM3045159: Healthy_unstimulated2; Homo sapiens; RNA-Seq
library_construction_protocol: CD14+ monocytes were isolated from umbilical cord blood using Ficoll-Isopaque density gradient centrifugation and positive magnetic bead selection (Miltenyi Biotec). RNA was extracted using an Rneasy microkit with on column DNAse digestion. SMARTer stranded total RNA pico kit (Takara) was used for library preparation. After library consruction, samples were pooled in equimolar concentrations and were sequenced on an Illumina HiSeq machine according to the manufacturer's guidelines.