title: GSM2794664: U87-control-2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from U87, U87 ERβ-KO, U87 ERβ-KO cells expressing ERβ1 and ERβ5 isoforms using RNeEasy mini kit using manufacturer's protocol (Qiagen, Valencia, CA). Total RNA samples about 1-2 µg were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.