title: GSM4586025: Chromatin-associated RNA-Seq siINTS1 CPSF73-AID IAA rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Isolated nuclei were lysed into an insoluble chromatin pellet and soluble nucleoplamic supernatant. Chromatin-associated RNA was then purified from the insoluble chromatin pellet using Trizol reagent (see Eaton et al. 2018 for detailed method). Isolated nuclei were lysed into an insoluble chromatin pellet and soluble nucleoplamic supernatant. Chromatin-associated RNA was then purified from the insoluble chromatin pellet using Trizol reagent (see Eaton et al. 2018 for detailed method). Chromatin-associated RNA was depleted of ribosomal RNA using Illumina Ribo-Zero (Illumina #20020597). Library was constructed using Illumina TruSeq® Stranded Total RNA (Illumina #20020597).