title: GSM4699141: TTseq.b0108; Drosophila melanogaster; OTHER
library_construction_protocol: Total RNA was isolated using TRIzol (Ambion) from S2 cell pellets vortexed for 30 min, extracted with ethanol precipitation, and subsequently purified with PureLink RNA Kit columns (Invitrogen). TT-seq libraries were constructed using the dUTP method (Parkhomchuk et al., 2009).