title: GSM2443539: mESC_Rsf1_ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde. Chromatin was disrupted into 100-250 bp fragments by sonication and precipitated using antibody and protein A beads as indicated in the text and protein A beads. After elution from the beads and reversing the cross-linking, the DNA fragments in immunoprecipitates were extracted with phenol/chloroform and recovered by ethanol precipitation. Immunoprecipitated DNA was ligated with 3’- and 5’-adapters for amplification and sequencing by using Illumina II.