title: GSM4648158: wild type TILs; Mus musculus; RNA-Seq
library_construction_protocol: B16-OVA tumors were harvested from Maoa WT and Maoa KO mice to prepare TII suspensions (10 tumors were combined for each group). TII suspensions were then sorted using a FACSAria II flow cytometer to purify immune cells (gated as DAPI-CD45.2+ cells). Sorted TIIs were immediately delivered to the Technology Center for Genomics & Bioinformatics (TCGB) facility at UCLA for library construction and sequencing. Cells were stained with trypan blue (Thermo Fisher Scientific, T10282) and counted using a Cell Countess II automated cell counter (Thermo Fisher Scientific). 10,000 TIIs from each experimental group were loaded on the Chromium platform (10X Genomics) and libraries were constructed using a Chromium Single Cell 3' library & Gel Bead Kit V2 (10X Genomics, PN-120237) according to the manufacturer's instructions.