title: Listeria monocytogenes Strain J2-1091
library_construction_protocol: SMRTbell sequencing libraries were generated using standard procedures (version C2; http://www.pacb.com/samplenet/PC_10kb_Template_Preparation_and_Sequencing.pdf). gDNA was sheared to an average size of approximately 10 kb using g-TUBEs (Covaris).  Fragmented DNA underwent DNA Damage Repair, End Repair, and blunt hairpin adapters were ligated onto each end.  Incompletely formed SMRTbell templates were degraded with a combination of Exo III and Exo VII.  SMRTbell templates were magbead loaded and sequenced with C2 chemistry.