title: GSM2611103: Q111 SST rep3; Mus musculus; RNA-Seq
library_construction_protocol: Cells were washed three times with ice-cold DPBS, scraped on ice and centrifuged. The pellets were flash-frozen with liquid nitrogen and stored at -80°C until needed.  RNA from striatal cell lines was extracted with the RNA/DNA/Protein Purification Kit (Norgen Biotek Corp., 23500), treated with DNase I and quantified with Nanodrop 2000 (Thermo Scientific). RNA quality and integrity were evaluated using the Fragment Analyzer™ (Advanced Analytical). All samples had an RNA Quality Number (RQN) higher than 9.8. 1 µg of total RNA was used for library preparation. Libraries were generated using the TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101), according to the manufacturer’s instructions.