title: GSM1467839: Huvec_Adar1kd_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from HUVECs previously transfected with siADAR1, siADAR2 or scrambled siRNAs using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. The cDNA and the final sequencing libraries were prepared as described before (Wang et.al, EMBO Mol Med, 2013). The libraries were sequenced using the Illumina HiSeq 2000 for 1 x 51cycles following the TruSeq RNA protocol. Libraries were prepared according to Illumina's instructions.