title: GSM3657324: pool.12_tumor.1432L; Homo sapiens; OTHER
library_construction_protocol: Resistant LNCaP/AR xenograft tumors were harvested and the genomic DNA was isolated by two rounds of phenol extraction using PhaseLock tubes (5prime) followed by isopropanol precipitation. All genomic DNAs from tumors, plasmid or pregraft cells were PCR amplified using a protocol previously described (Zuber NBT 2011). Libraries were analyzed on an Illumina HiSeq 2500 and 18 nucleotides of the guide strand were sequenced using a custom primer as previously described (Zuber Nature 2011).