title: RNAseq-St40_R1
design: RNA isolation for embryonic limb bud, mature upper arm and upper arm blastema RNA-seq were performed from same cell pools used for ATAC-seq samples (see ATAC-seq library preparation).  Around 5 x104 cells were spun down (450 x g for 10 min at 4). Total RNA pools were isolated using an RNA extraction kit (in-home). After RNA isolation, RNA qualities from each sample were verified by High-sensitive total RNA kit of Fragment analyzer (Agilent). Library preparations were generated using Smart-seq v2 protocol and sequenced by Nova-seq S1 (SR100)