title: GSM2443533: HeLa_RSF1_KD_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Samples for mRNA were prepared following the Illumina mRNA sequencing sample preparation guide. Briefly, total RNA was extracted with Trizol (Cat # 15596026, Invitrogen, Carlsbad, CA, USA). mRNA was specifically enriched from total RNA using oligo(dT) beads and hydrolyzed into small pieces. The fragments were then reverse-transcribed into first-strand cDNA using random hexamer primers, followed by second strand synthesis using DNA polymerase I. The short cDNA strands were ligated with 3’- and 5’-adapters for amplification and sequencing (Illumina HiSeq v4 (pair end,50 bp, 50 M reads) in the HudsonAlpha Institute for Technology (Huntsville, AL, USA). Primer sequences are available upon request.