title: GSM4760355: RIP_ESC_input; Mus musculus; RIP-Seq
library_construction_protocol: 5×10^6 control ES cells per reaction were cross-linked in 0.3% formaldehyde and neutralized by 1×glycine. The nuclei were isolated and IP reaction was performed using Magna Nuclear RNA-Binding Protein Immunoprecipitation Kit. RNAs of input and IP samples were extracted using TRIzol Reagent and chloroform coupled with isopropanol precipitation. RNAs extracted from input and IP samples were subjected to library generation using VAHTS Total RNA-seq (H/M/R) Library Prep Kit.