title: RNA-Seq of Vero cell culture: Sus scrofa domesticus faeces
design: Purified total RNA was subjected to retrotranscription with SuperScript III Reverse Transcriptase (Invitrogen, Carlsbad, California, USA) and NEBNext mRNA Second Strand Synthesis Module (Euroclone, Stockholm, Sweden) or with Maxima H Minus Double-stranded cDNA synthesis kit  (ThermoFisher Carlsbad, California, USA). Doublestranded cDNA was purified with Agencourt AMPure XP (Beckman Coulter, Brea, California, USA) and quantified with Qubit dsDNA HS assay kit (ThermoFisher Carlsbad, California, USA). The cDNA library was prepared using Illumina Nextera XT DNA Sample Preparation kit (Illumina, San Diego, California, USA), and fragments were selected with Agencourt AMPure XP (Beckman Coulter, Brea, California, USA). Library was checked for quality and size with Agilent 2100 Bioanalyzer (Agilent High Sensitivity DNA kit, Agilent Technologies, Carlsbad, California, USA), and sequenced with Miseq v2 or v3 Reagent Kit (250 or 300PE) using Illumina MiSeq platform.