title: GSM3044955: Deep-layer astrocytes rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from sorted cells with TRIzol (Invitrogen) or RNAiso Plus (Takara) and was then subjected to reverse transcription with the use of a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). RNA libraries were prepared using the SMARTer Ultra Low Input RNA for Sequencing kit (Clontech Laboratories) followed with Nextera XT DNA Library Prep kit (Illumina) and RNA sequencing performed using the Illumina HiSeq 2500 platform.