title: RNAseqofOryziasMelastigma:male liver
design: The total RNA of the livers from three replicates was extracted by Trizol. Each replicate contained three fishes. The quality of total RNA was further determined with an Agilent 2100 Bioanalyzer, the concentrations were > 200ng / L. 260/280 ratios were > 1.8, the rRNA Ratio [28s/18s] > 0.9, and RIN > 5, which correspond with the sample quality requirements of total RNA for Illumina sequencing.