title: GSM4648437: Lung_OVA+SIA; Mus musculus; RNA-Seq
library_construction_protocol: The  lungs of 3 mice were pooled for the sample Lung_OVA+SIA, 16 hind ankle joints (8 mice) were pooled for the sample Synovium_OVA+SIA and 24 hind ankle joints (12 mice) were pooled for the sample Synovium_SIA. Eosinophils were sorted via the markers CD45+ CD11b+ Siglec-F+. For each sample 8000 cells were loaded onto a Chromium Single Cell A Chip (10x Genomics) with Chromium Single Cell 3' v3 Gel Beads (10x Genomics) to generate single cell bead emulsions for reverse transcription and cell bar coding according to the manufacturers instructions Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead kit v3 and i7 Mutiplex kit (10X Genomics) according to the manufacturers instructions. Libraries were sequenced on an Illumina HiSeq 2500 using the recommended read lengths for Chromium Single Cell 3' v3 chemistry.