title: GSM1831876: Chat6_GFP-Nova_CLIP_2; Mus musculus; RNA-Seq
library_construction_protocol: HITS-CLIP was performed on freshly isolated spinal cords using anti-GFP antibodies. The monoclonal anti-GFP antibodies 19C8 and 19F7 from Memorial Sloan Kettering Antibody Facility were used. partial RNAse digestion, 3' and 5' RNA linker ligation and two rounds of PCR amplificiation