title: GSM3656843: Human pylorus organoids 1; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were removed from Matrigel and resuspended in RTL buffer (Qiagen). RNA was extracted from the organoids using the RNeasy Mini RNA Extraction Kit (Qiagen). Illumina TruSeq Stranded mRNA Library Prep Kit (Cat#20020594) was used with 500 ng of total RNA for the construction of sequencing libraries. Libraries were prepared for sequencing according to the manufacturer's protocol using 11 PCR cycles.