title: GSM4648417: E133; Anas platyrhynchos; miRNA-Seq
library_construction_protocol: Total RNAs of six samples were extracted and purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Agilent 2100 with RIN number >7.0.  All samples were kept at -80 °C. Libraries were prepared for sequencing using standard Illumina protocols