title: GSM1649950: root_1; Zostera marina; RNA-Seq
library_construction_protocol: RNA extraction was performed using the InviTrap Spin Plant RNA Mini Kit (Stratek Molecular) following the manufacturer's protocol. RNA  integrity was checked with an automated electrophoresis station, Experion (Bio-RAD), using StdRNA chips and reagents (Bio-RAD). RNA concentrations ranged between 23-182 ng/µl, RQI values were >7.2. Library preparation involved DNase 1 digestion of total RNA, mRNA isolation by use of oligo(dT) beads, mRNA fragmentation, first and second strand cDNA synthesis, end-repair, A-tailing, bar-coded adapter ligation and PCR amplification. Sequencing libraries were checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) before sequencing. Paired-end (2 x 100 bp) RNA Sequencing (RNASeq) data were generated using standard Illumina protocols and kits (TruSeq SBS KIT-HS v3, FC-401-3001; TruSeq PE Cluster Kit v3, PE-401-3001) and all sequencing was performed using the HiSeq 2000 platforms.