title: GSM3043226: E47KO_SM_3weeksDex_rep3 [GAR0289]; Mus musculus; RNA-Seq
library_construction_protocol: For longterm Cort-treated liver tissue and longterm Dex-treated liver, skeletal muscle and white adipose tissue, RNA was extracted using the Qiazol protocol. For acute Dex-injected liver tissue RNA was extracted using the RNeasy minikit (Qiagen). All samples were DNAse-treated using the Ambion DNase I kit (Thermo Fisher Scientific). The quality of the RNA was verified by Agilent 2100 Bioanalyzer with RNA 6000Nano Reagents (Agilent Technologies). Library preparation and rRNA depletion was performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) or unstranded mRNA Library Prep Kit (Illumina) according to manufacture's manual. 500 ng RNA were used as input for each sample.