title: GSM2611560: J37iS_ESC_H2AX; Mus musculus; ChIP-Seq
library_construction_protocol: Native  Chromatin  immunoprecipitation  (N-ChIP)  assay  was  performed  as  previously described. Approximately 10  million  of  ESC s  were  used  for  each  ChIP  and massive  parallel  sequencing  (ChIP-Seq)  experiment.  In brief, chromatin  pellets  were  briefly digested  with  MNase  (New  England  BioLabs) and  the  mononucleosomes  were  monitored  by electrophoresis.5ug  anti-H2A.X antibodies (active motif 39689)were  used  per  ChIP experiment. Libraries were prepared according to Illumina's standard protocol, with instructions accompanying the DNA Sample Kit