title: GSM2616309: H3K4me3 GIC-387 shIDH1-89; Homo sapiens; ChIP-Seq
library_construction_protocol: cells per sample were fixed with 1% formaldehyde and ChIP-seq was performed according to previous studies (Chen et al., 2015; doi: 10.1016/j.cell.2015.07.042 and Chen et al. 2015; doi: 10.1101/gad.246173.114) ChIP-sequencing libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250- 450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.