title: GSM4648255: RNA_C1_dKO_REP1; Mus musculus; RNA-Seq
library_construction_protocol: For each cell line, three independent RNA samples were isolated at different time points. The total RNA was isolated from cells using the RNeasy Kit (Qiagen). The Qiagen RLT lysis buffer was additionally supplied with β-mercapthethanol (10μl per 1ml buffer). The cell lysates were thoroughly homogenized by passing through syringe with 20-G needle. The concentration and purity of the extracted RNA was measured on Qubit 3 Fluorometer (Thermo Fisher Scientific). For each cell line, three independent RNA samples were isolated at different time points. The further analyze of RNA quality and integrity was assessed on Bioanalyzer 2100 (Agilent Technologies), using the Eukaryote Total RNA Nano assay. The subsequent libraries preparation and Next Generation Sequencing (NGS) on the NextSeq500 platform (Illumina) was performed in collaboration with Laboratory of Functional Genomics, Nutrigenomics and Systems Biology, Max Delbrück Center for Molecular Medicine, Berlin (Dr. Tatiana A. Borodina)