title: acid peat and stalagmite Raw sequence reads
design: The V4 region of the 16S rRNA gene was amplified using SYBR?Premix Ex Taq? with 0.25 ŚĚM final concentration of universal prokaryotic primer set, 806R and U519fM. The reverse ampli?cation primer (U806r) used for the second round of bacterial PCR included a unique 12 base Golay barcode sequence. barcoded PCR products were pooled and purified with the AMPure?XP PCR purification kit (Agencourt Bioscience Corp., Beverly, MA). Four hundred nanograms of the mixed and purified barcoded amplicons were subject to Illumina MiSeq sequencing at the W. M. Keck Centre for Comparative and Functional Genomics, University of Illinois at Urbana-Champaign