title: GSM2615730: Sbi._R.07020_60DAP_internode_2.3; Sorghum bicolor; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol according to the manufacturer’s protocol (Invitrogen). About three µg of total RNA was used for RNA-seq library preparation using TruSeq™ RNA Sample Prep Kit v2 (Illumina, Inc.). RNA-seq library quality was assessed using a Bioanalyzer and sequenced on an Illumina HiSeq 2500 at the Texas A&M University Genomics and Bioinformatics Service Center. The sequence template was prepped using the True-seq V2 kit from illumina which isolates polyadenylated RNA and preps it for sequencing.