title: Bacterial microbiome in gorgonian corals: Sample k11ppd
design: DNA extracted from gorgonian tissues using the Promega Wizard Genomic DNA Purification Kit. The bacterial 16S rRNA gene was PCR amplified with 8-bit indexed primers on 96-well plates (see Kozich et al. 2013. Appl Environ Microbiol 79(17): 5112-5120.). The PCR product was normalized to 25 nanograms/L per sample with the SequalPrep Normalization Plate Kit (Life Technologies).