title: WGS of United States invasive Streptococcus pyogenes isolates
design: Shearing protocol - 500 bp protocol using sonication (Covaris) Library preparation kit - Ovation Rapid (Nugen) Index - dedicated read Library QC    KAPA qPCR quantification (KAPA Biosystems) Sequencing instrument - Miseq Sequencing kit - Illumina MiSeq Sequencing kit v2 (500 cycles)