title: CN115125_WGBS_Buds
design: Genomic DNA was isolated from leaf and bud tissue of B. nigra CN115125 nuclei using Qiagens DNeasy Plant Kit (Qiagen GmbH, Hilden, Germany) following the manufacturers protocol. Zymo Research EZ DNA Methylation kit was used for bisulfite conversion on 100ng of DNA along with 0.5% w/w of unmethylated lambda DNA(Promega,US)