title: Illumina HiSeq 2000 paired end sequencing; Regulation of constitutive and alternative messenger RNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength
design: Regulation of constitutive and alternative messenger RNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength
library_construction_protocol: Human Cal51 breast carcinoma cells were treated with either control siRNA or PRPF8 siRNA and harvested 54 hours post-transfection for total RNA. 4 experimental replicates for PRPF8 siRNA and 3 experimental replicates for Control siRNA are provided. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers protocol. Illumina's TruSeq RNA-seq kit was used to prepare libraries for sequencing according to manufacturers protocol.