title: CHM13 Illumina WGS
design: CHM13htert gDNA was sheared using Covaris S2 with cycling conditions of 10% Duty cycle, Intensity 4, Cycles/Burst 200, and Time 100s. The sheared DNA was then end-repaired using NEBNext End Repair Module (P/N: E6050L). Repaired sheared DNA was then A-tailed and Y-adapters were ligated. The library was size-selected at a range of 450-550 bp then sequenced using Illumina HiSeq PE-101 from two lanes to generate ~31-fold sequence coverage. Reads were aligned to GRCh38 (excluding alternate haplotypes) with BWA-MEM v0.7.3, processed with samblaster to mark duplicate reads, and sorted with samtools.