title: GSM2445477: Combined A+B rep2; Homo sapiens; miRNA-Seq
library_construction_protocol: HEK293 cells were washed by PBS, then centrifuged at 800 rpm for 5 min. 15 μg of total RNA for each sample was used for the construction of libraries using a Small RNA Library Preparation kit (Illumina Inc., San Diego, CA, USA) according to the manufacturer’s protocol. Each library was barcoded with different indices and stored in a −80 °C freezer before sequencing. Sequencing was performed in a v3 flowcell on an Illumina HiSeq 2500 sequencer, generating SE:1×50bp reads.