title: Pooled sample consisting of samples: Cyst 649 S1|Cyst 602 S1|Cyst 626 S1|Cyst 642 S1|Cyst 611 S1|Cyst 612 S1|Cyst 684 S1|Cyst 605 S1|Cyst 613 S1|Cyst 693 S1|Cyst 630 S1|Cyst 654 S1|Cyst 662 S1|Cyst 670 S1|Cyst 686 S1|Cyst 639 S1|Cyst 671 S1|Cyst 640 S1|Cyst 664 S1|Cyst 672 S1
design: Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument