title: GSM4648043: Plasmacytoid dendritic cells from recovered patient 30916; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were FACSorted in Lysis Binding Buffer and mRNA was extracted through Dynabeads polyT RNA was sample-barcodad by a RT reaction. After, cDNA was purified with SPRIselect and amplified using the T7 promotor as template. RNA molecules were fragmented and purified again. Afterwards, a ssRNA adaptor was ligated to the 3'-end of the RNA fragments and, finally, cDNA was amplified.