title: GSM1181876: pooled total RNA subjected to "long" running  conditions for two dimensional gel electrophoresis; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: Hot phenol extraction of total RNA, followed by pooling of RNA from all 41 growth conditions, then two dimensional acrylamide gel electrophoresis and excision of arc corresponding to circular RNA (see Supplemental Methods in  www.pnas.org/lookup/suppl/doi:10.1073/pnas.1218353110/-/DCSupplemental) Same as that of Quail et al. Nature Methods 2008 (PMID: 19034268), with slight modifications (see Supplemental Methods in  www.pnas.org/lookup/suppl/doi:10.1073/pnas.1218353110/-/DCSupplemental)