title: GSM1180729: wild-type passage 1 R1; Mus musculus; RNA-Seq
library_construction_protocol: For total RNA, 10e6 cells were harvested in Trizol according to the manufacturers instructions. Quality control of total RNA was first performed using the RNA Qubit Assay (Invitrogen) and the Bioanalyzer RNA Nano 6000 Chip Kit (Agilent).  Samples containing at least 100ng of total RNA and a Bioanalyzer RNA Integrity Number of >7.0 passed quality control and proceeded into library construction. Library construction was performed using Illumina's TruSeq RNA Library Prep Kit according to the manufacturer's protocol, with 100ng of total RNA as input for each sample.  Final library quality control was performed using the DNA High Sensitivity Qubit Kit (Invitrogen), the Bioanalyzer High Sensitivity Chip Kit (Agilent) and the 7900HT Fast qPCR machine (Applied Biosystems). qPCR was performed  using the Illumina Universal Library Quantification Kit from KAPA Biosystems.  RNASeq libraries were then normalized to 2nM, pooled for multiplexing in equal volumes, and sequenced at 10pM on the Illumina HiSeq 2000.