title: GSM4760672: Patient Sample, C002_d21; Homo sapiens; RNA-Seq
library_construction_protocol: We sorted CD8+ T cells from blood samples obtained from hematopoietic cell transplant patients in the placebo-control arm of the ABA2 clinical trial (NCT# NCT01743131) RNA was isolated from CD4 and CD8 sorted T cells for each patient using the RNeasy Micro kit  (Qiagen) with on-column DNase digestion. RNA quality was assessed using an Agilent  Bioanalyzer and two nanograms of total RNA was used as input for cDNA synthesis using the  Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer's  instructions. Amplified cDNA was fragmented and appended with dual-indexed bar codes using  the NexteraXT DNA Library Preparation kit (Illumina). Libraries were validated by capillary  electrophoresis on an Agilent 4200 TapeStation, pooled at equimolar concentrations, and  sequenced on an Illumina HiSeq3000 at 100SR, yielding approximately 20 million reads per  sample.