title: GSM3206939: autocatalytic_circGFP_rep2; Homo sapiens; OTHER
library_construction_protocol: Total RNA was isolated from HeLa cells using Qiagen RNeasy columns. RNA was ribo-/polyA- selected and treated with RNase R to remove linear RNA. Circular RNA was then fragmented to ~30-60 nts, and m6A residues were isolated by crosslinking anti-m6A antibody to fragmented RNA. RNA ligation for library construction was performed on affinity beads and RNA was subsequently transformed into dsRNA libraries using RT, cDNA circularization, and PCR amplification.