title: GSM3890749: A549 RB1 knockout replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA was extracted by RNAeasy plus mini kit. Then, the mRNA and cDNA were captured from by NEBNext® Ultra™ Directional RNA Library Prep Kit. Finally, the cDNA was transferred to Illumina 2500 sequencer for RNA sequencing. The mRNA and cDNA were captured from by NEBNext® Ultra™ Directional RNA Library Prep Kit.