title: DWTR(50mg/L)
design: The bacterial 16S rRNA V4-V5 fragments were amplified using forward primer 515F (5-GTGCCAGCMGCCGCGGTAA-3) and reverse primer 907R (5-CCGTCAATTCCTTTGAGTTT-3). PCR reaction was performed in the 50 L mixture that contained: 0.2 M of primer 515F, 0.2 MTimes New Roman