title: NextSeq 500 sequencing; Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro.
design: Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro.
library_construction_protocol: Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol.  DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing.