title: GSM4648939: Con2_6_LYQ; Homo sapiens; RNA-Seq
library_construction_protocol: PTC tissue and normal thyroid tissue were collected during surgery and  flash frozen by  liquid nitrogen. Total RNA of tissue sample was extracted using Trizol, according to manufacturer's procedure, quantified and quality controlled using a 2100 Bioanalyzer (Agilent). RNA samples with RNA Integrity Number (RIN) above 7 were depleted for ribosomal RNA and converted into cDNA library using a TruSeq Stranded Total Library Preparation kit (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols