title: GSM2966329: sgC_3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent and purified using Omnigen RNA extraction kit. PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X76) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).