title: 16S amplicon of soil metagenome: extracted soil community cultivated on agar plates, 1:1000 dilution, replicate 5
design: extracted soil community spread on agar plates in 1:1000 dilution, plates incubated for 1 month, biomass washed of 7 plates and pooled, mechanical cell lysis by bead beating, phenol DNA extraction, DNA precipitation with ethanol, Q-Sepharose purification for removal of humic acids, PCR of V3-V4 region with 314F and 758R primers, amplicon purification and quantification, pooling, further library prep as in standard illumina instructions