title: GSM3045689: LY6E Rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using RNeasy Mini Kit (Qiagen #74106) following the manufacturer's instructions. Integrity was verified using the Agilent 2100 Bioanalyzer. All samples had a RIN score of 8 or higher. Four micrograms of total DNAse-treated RNA were then prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina (Illumina  #RS-122). Poly-A RNA was purified and fragmented before strand specific cDNA synthesis.  cDNA were then A-tailed and indexed adapters were ligated. After adapter ligation, samples were PCR amplified and purified with AmpureXP beads, then validated again on the Agilent 2100 Bioanalyzer. Before being normalized and pooled, samples were quantified by Qubit then run on the Illumina Hiseq 2500 using SBS v3 reagents.