title: GSM1833589: opossum_FL_ES_rep1; Monodelphis domestica; RNA-Seq
library_construction_protocol: Embryonic mice, opossums, bats and pigs with early (ES) or late (LS) stage forelimbs were obtained from a variety of sources. Forelimbs for the ES were harvested at Stage 14 for bat, E11 for mouse, Stage 28 for opossum and E22 for pig. For the LS, forelimbs were harvested at Stage 15 for bat, E12 for mouse, Stage 29 for opossum and E26 for pig. Limbs were removed from embryos and stored in RNALater in -20° C until further processing. RNA was extracted from tissues using E.Z.N.A. Total RNA Kit I (OMEGA bio-tek #R6834), and converted into RNASeq libraries with the Illumina TruSeq RNA Sample Preparation Kit (Illumina RS-122-2001) Libraries were sequenced on an Illumina HiSeq 2500 housed in the Roy G. Carver Biotechnology Center at the University of Illinois.