title: GSM2792666: NMR_Bis_PCR_2; Heterocephalus glaber; OTHER
library_construction_protocol: Targeted bisulfite PCR-based analysis was performed using the 48.48 layout on the Fluidigm C1 system (Fluidigm, USA), coupled with Illumina NextSeq sequencing. Bisulfite PCR primers were designed using Primer3 (PMID:17485472), DNA was bisfulite converted (EpiTect, Qiagen) prior to amplification. Amplicons from a single sample were pooled and individually indexed to create a multiplex library prior to sequencing on an Illumina NextSeq instrument.