title: GSM3041623: GFP_NEG_c01_r25; Homo sapiens; RNA-Seq
library_construction_protocol: GFP-positive and -negative cells were sorted and entered into the Fluidigm C1 HT workflow, which was used to capture individual cells, lyse, reverse transcribe, and prepare libraries for sequencing (See: Using the C1 High-Throughput IFC to Generate Single-Cell cDNA Libraries for mRNA Sequencing, PN 100-9886). Sequencing was performed on a Nextseq 500 using a high-output kit, resulting in a total of 475 million, paired-end, 75 bp reads.