title: GSM3891736: RelA-TA2_CI-dTA1  MEFs TNF 3hr; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated with the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. RNA concentrations were quantified using Epoch Spectrophotometer System (Biotek). 1 ug of total RNA was subjected to library synthesis using KAPA Stranded mRNA-Seq Kit. 1 ug of total RNA enriched for polyA+ mRNA and reversed transcribed to double-stranded cDNA. The cDNA was end repaired, adenylate, and ligated with Illumina indexes prior to 12 cycles of PCR amplification. Sequencing was performed by the Broad Stem Cell Research Center core facility at the University of California, Los Angeles with single-end 50 bp sequencing