title: WGS of Culex pipiens
design: DNA was extracted from individual mosquitoes for all Eurasian samples using the DNA Invisorb Spin Tissue Mini Kit (Invitek, Berlin, Germany) and for all American samples using the Qiagen Blood and Tissue Kit (Qiagen, Germantown, MD, USA). DNA concentration was determined by nanodrop; ~50 ng of DNA from each individual mosquito was sent for sequencing to the Fasteris company (Fasteris Inc., Switzerland).