title: RNA-seq WTC1 liver 1 dataset
design: Embryonating domesticated and wild turkey eggs were assigned to one of 4 treatment groups (aflatoxin B1 1 day exposure (A1), control 1 day exposure (C1), aflatoxin B1 5 day exposure (A5), control 5 day exposure (C5)) and incubated under standard conditions.  In ovo exposures were performed by injection of aflatoxin B1 (1 µg) or control solution (30% EtOH) into the air sac on developmentally equivalent days (day 17 in domesticated, day 19 in wild).  Embryonic tissue samples from the liver and spleen were collected after 1 day or 5 days of exposure.  Indexed libraries were constructed from DNase-treated total RNA and sequenced on the Illumina HiSeq 2000 by the University of Minnesota Genomics Center to produce 101-bp paired-end reads.  For the wild turkey, 15 pairs of matched samples (liver and spleen) were sequenced (N=30, flowcell 1) with 4 libraries/treatment group/tissue, except for the A1 group (n=3/tissue).