title: GSM1186185: IgH GF LP mouse 3; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow was extracted by crushing femurs and passing through 70 micron filter. Splenocytes were prepared by pushing through 70 micron filter. Lamina propria lymphocytes were prepared by washing cleaned, minced small intestinal samples in EDTA solution followed by collagenase extraction of lymphocytes. RNA prepared from Trizol was extracted and subjected to 5' RACE using either IgH C mu or IgK C kappa primers.