title: GSM4760623: HC-4; Homo sapiens; RNA-Seq
library_construction_protocol: The PBMCs were used for single-cell RNA sequencing library preparation by Chromium Single Cell 3' Reagent Kit (version 2) and Chromium Controller (10X Genomics, CA, USA) according to the manufacturer's instructions. Firstly, the cDNA was digested into fragments of about 200-300 bp, and then library construction process of traditional second-generation sequencing such as sequencing adapter P5 and sequencing primer R1, and finally the DNA library was obtained by PCR amplification.contain the P5 and P7 primers used in Illumina bridge amplification. paired-end sequencing model