title: GSM2256691: DLA rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNEasy Mini-Kit (Qiagen) as per manufacturer’s protocol with minor modifications (Qiagen). Optimal-quality RNAs were considered for cDNA library preparation (RIN>8.0). 100ng total RNA was reversed-transcribed, and resulting cDNA was pre-amplified for 10 cycles. Tagmentation was carried out on 10ng cDNA using the Nextera DNA Sample Preparation Kit (Illumina). The tagmentation reaction was carried out at 55°C for 5 minutes and purified using PCR Purification Kit (Qiagen). Transposed cDNA was utilized for limited cycle enrichment PCR using v2_Adx.x barcodes (IDT). Libraries were amplified for 12 continuous cycles and resulting libraries were purified with AMPure XP beads (Beckman Coulter). Library quantification was done using KAPA for Illumina Sequencing Platforms (Illumina)