title: GSM2794503: Evx1as-/- S7; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of RNA-Seq were extracted by TRIzol reagent according to manufacturer's instruction. mRNA were further isolated by Dynabeads mRNA purification kit (Life Technologies) according to manufacturer's instruction. For embryo sections, embryo was freshly embedded in OCT and subjected to cryosection and followed by Geo-seq for spatial transcritpome analysis. Libraries were prepared by illumina TruSeq Stranded mRNA LT Sample Prep Kit (RS-122-2101) according to the standard protocols of the manufacturer.