title: NextSeq 500 paired end sequencing; Transcriptome wide mapping of Hfq mediated RNA-RNA interactions in E. coli (RIL-seq)
design: Transcriptome wide mapping of Hfq mediated RNA-RNA interactions in E. coli (RIL-seq)
library_construction_protocol: E .coli Strains MG1655 and HM34 were grown overnight in LB at 37 °C with shaking (200 r.p.m.), diluted 100-fold in fresh LB, and re-grown with shaking at 37 °C to log phase (OD600 = 0.5) RNA was extracted according to the standard LS-TriReagent protocol (Sigma) RNAtag-Seq protocol (Shishkin et al., 2015)