title: GSM1179565: ZHBTc4_+dox_Nanog; Mus musculus; OTHER
library_construction_protocol: The initial steps of the 4C procedure, as published before (Simonis et al., 2006, Nature Genetics), remain unchanged. Cells are cross linked using 2% formaldehyde for 10min at room temperature in PBS 10%FCS, nuclei are isolated, after which chromatin is digested with HindIII and subsequently ligated under diluted conditions. After reversal of the cross links the DNA is purified and treated with the second restriction enzyme treatment (indicated in the sample description). After a second re-ligation step the sample is purified and a 4C PCR is applied.