title: GSM3891510: IL6 (20ng/ml) treated_12hrs 12-V-2; Mus musculus; RNA-Seq
library_construction_protocol: We performed Trizol-based extractions in accordance to the manufacturer's protocol with several modifications. 2ul of linear acrylamide (Cat No AM1354) was added before addition of 100% isopropanol. After the 75% ETOH wash, the samples were resuspended in 87.5ul of water and a mixture of 10ul of buffer RDD and 2.5ul of DNase I from Qiagen. Samples were left at room temp for 15min to allow the DNAse digestion to proceed. The samples were then processed and passed through Qiagen MinElute columns in accordance tro the manufacturer's protocol. RNA quantity and quality were determined using Agilent's RNA 6000 Pico Chip RNA-seq libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, 20020598). The sequencing was performed using the Illumina NovaSeq. RNA-seq libraries were prepared and sequenced by the Genome Technology Core at New York University School of Medicine.