title: GSM2444067: 14317A_input control; Rattus norvegicus; MeDIP-Seq
library_construction_protocol: Genomic DNA was isolated using the DNeasy Kit (Qiagen, CA) according to the manufacturer’s protocols. Libraries were prepared according to the manufacturer’s protocol, the fragmented DNA were end-repaired, A-tailed, ligated adapter and purified using Agencourt AMPure XP kit. 0.4µg DNA was used for subsequent MeDIP enrichment. Briefly, immunoprecipitation was carried out at 37˚C for 0.5 hr to 1 hr. After amplification and purification, we performed agarose gel electrophoresis and excised bands from the gel to produce libraries with insert sizes of ~125bp, then quantified these libraries using KAPA DNA Reagent and Kits (KAPA Biosystem). The total libraries randomly arranged in 8 lanes were sequenced by the Next Generation Sequencing.