title: GSM2446007: Control_4d_2; Hebeloma cylindrosporum; RNA-Seq
library_construction_protocol: Lyophilized mycelia were ground in liquid nitrogen and total RNA was extracted using  the "RNeasy Plant Mini Kit" (Qiagen, Courtaboeuf, France).  PEG 8000 (20 mg.ml-1) and β-mercaptoethanol (10 µl.ml-1) were added in the RLC buffer (Qiagen).   Samples were subsequently purified using the "Total RNA Purification, NucleoBond® RNA/DNA 80" kit  (Macherey-Nagel, Hoerdt, France) by following the manufacturer’s recommendations. cDNA libraries were prepared for sequencing using standard Illumina protocols by the Joint Genome Institue (JGI)