title: GSM3207009: Nuclear Fractionation 1; Homo sapiens; RNA-Seq
library_construction_protocol: To validate the NLS APEX-seq data we carried out nuclear fractionation of HEK 293T cells by following an established protocol (Gagnon et al., Nature Protocols 2014). We modified the protocol so that the extracted RNA was not purified by trizol extraction, but rather by using the RNeasy plus mini kit (Qiagen). RNA-seq was carried out using the same protocol as that used for APEX-seq, thereby generating polyA-selected libraries for the nuclear and cytoplasmic fraction using Illumina TruSeq kit.