title: GSM2443464: ROX2_2; Oryza sativa; RNA-Seq
library_construction_protocol: Total RNA was extracted from the roots of 2-week-old ROX plants and NT rice seedlings grown under normal growth conditions using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction. A modified TruSeq method was used to construct a strand-specific RNA-seq library with different index primers and libraries were sequenced in the Illumina HiSeq 2500 system in MACROGEN. Cutoff criteria to eliminate genes that showed no significant difference were more than 2 fold up- and down-regulation in ROX plants compared to NT (Ilmi).