title: GSM1173409: Neuronal nuclei from P21 hypothalamus_rep1; Mus musculus; MRE-Seq
library_construction_protocol: Genomic DNA was isolated with phenol/chloroform extraction followed by ethanol precipitation Libraries were constructed by MSA-seq. As a brief description of the MSA: 500ng of genomic DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification (Waterland et al, 2010 Plos Genetics). MSA products were sheared to 200-500bp, ligated to sequencing adaptors for paired-end read, and submitted for sequencing by Illumina HiSeq.