title: GSM1651919: 6hrs_RA_2; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in TRIzol and RNA was isolated using Directzol RNA miniprep kit( R2052). Quality of RNA were assessed using bioanalyzer and samples with RIN more than 9 were used for library preparation. For each sample, 250ng of total RNA was used to make libraries. Following manufacturer's directions, short fragment libraries were made using the Illumina TruSeq Stranded Total RNA LT - (with Ribo-Zero TM Gold) - set A and B (Illumina, Cat. No. RS-122-2301 and RS-122-2302). The resulting libraries were quantified using the LabChip GX (Perkin Elmer) and a Qubit Fluorometer (Life Technologies). All libraries were pooled, quantified and run as 50 bp single read on an Illumina HiSeq 2500 instrument using HiSeq Control Software 2.2.58