title: GSM1833874: ChIP-seq H3K27Ac_DMSO; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin from formaldehyde-fixed cells (5 × 106 cells per histone mark) was fragmented to a size range of 200–700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with an antibody against H3K27ac (2.5 μl; ActiveMotif, ab4729), and enrichment for H3K27ac was confirmed by dot blot as described (Ernst et al. 2011). Antibody-chromatin complexes were pulled down with protein G magnetic beads (Dynabead, 10003D), washed and then eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was treated with RNase and purified with Agencourt AMPure XP (Beckman Coulter A63880). Libraries were prepared according to Illumina's instructions.