title: GSM2241687: prt1-1, 37C, soluble; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells were lysed using a cryogenic mixer mill. Soluble and membrane-bound fractions were separated by centrifugation. SRP72-TAP was immunoprecipitated from the soluble fraction. PolyA RNA was recovered by each fraction using oligo-dT beads and fragemented by alkaline hydrolysis. RNA fragments of approximately 40 nucleotides were extracted from a denaturing polyacrylamide gel. A DNA adapter (CTGTAGGCACCATCAAT) was ligated to the 3' of fragments and used to prime reverse transcription. cDNA were circularized and libraries were barcoded during PCR amplification.