title: GSM2257686: AEG_1CKO_chow_3231 biological rep2; Mus musculus; RNA-Seq
library_construction_protocol: Trizol extraction of total RNA was performed using QIAGEN columns according to the manufacturer's instruction. Total RNA, extracted using Qiagen miRNAeasy mini kit (Qiagen, Valencia, CA) from livers of 3 adult mice per group, was employed for RNA sequencing. RNA-Seq library was prepared using Illumina TruSeq RNA sample preparation kit and sequenced on Illumina HiSeq2500 platform per manufacturer's protocol.