title: RNA-seq of Arabidopsis thaliana: control conditions, arp6 mutant
design: The total RNA samples were first treated with DNase I (NEB). Then the mRNA was enriched by using the oligo(dT) magnetic beads (Invitrogen) and fragmented into 130bp fragments with First Strand buffer. Next, the first strand of cDNA was synthesized by using random hexamer-primer and SuperScript II reverse transcriptase (Invitrogen) (25oC for 10 min, 42oC for 40 min, 70oC for 15 min). Following the second strand synthesis (16oC for 1 h), the double strand cDNA was purified with Ampure XP Beads (Agencourt). Then, cDNA was combined with End Repair Mix and incubated at 20oC for 30 min. After purification, A-Tailing Mix was added and the mixture was incubated at 37oC for 30 min. Finally, sequencing adaptors were ligated to the fragments (20oC for 20 min) and the fragments were enriched by several rounds of PCR amplification. The PCR products were purified with Ampure XP Beads (Agencourt).