title: GSM1183057: XT32_BB3KD_polyA-RNA_2nd; Xenopus tropicalis; RNA-Seq
library_construction_protocol: Embryos were flash-frozen on dry ice, and total RNA was extracted using Trizol (Invitrogen) and subjected to two precipitations with ethanol and LiCl. Total RNA to the amount of two X. tropicalis embryos (~3 µg) was processed according to the Illumina TruSeq protocol (November 2010). The quality was controlled on the Bioanalyser 2100 (Agilent) using the DNA-specific chip DNA 1000 (Agilent). Libraries were read paired-end along 55 bases on the Illumina HiSeq 2000 machine.