title: GSM3891461: Control cDNA, rep 2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Approximately 100 ovaries from 0-4 day-old control or HP1a-SKD females were manually dissected in the cold PBS. Poly(A)+ RNA purified on the oligo(dT) column was isolated (in two biological replicates) using Trizol reagent (Invitrogen). Purification of poly(A)+ RNA on the oligo(dT) column, cDNA library preparation with random primers and single-end deep sequencing on Illumina HiSeq 1500 were carried out by the Genoanalytica (https://www.genoanalytica.ru) resulting in the 19-39 million 50 nt single-end reads.