title: GSM4586519: 14251X8: mct1del_12hr; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cultures were flash frozen and later total RNA was isolated using the Direct-zol kit (Zymo Research) with on-column DNase digestion and water elution. Sequencing libraries were prepared by purifying intact poly(A) RNA from total RNA samples (100-500 ng) with oligo(dT) magnetic beads and stranded mRNA sequencing libraries were prepared as described using the Illumina TruSeq Stranded mRNA Library Preparation Kit (RS-122-2101, RS-122-2102).