title: GSM2257765: non-selected strain PM diet liver biological rep2 [T17-5L]; Oncorhynchus mykiss; RNA-Seq
library_construction_protocol: Tissue samples were excised, flash-frozen in liquid nitrogen and stored at -80°C until usage. Samples were homogenized using a bead-beater with TRIzol reagent per the manufacturer's recommendation for total RNA isolation. Total RNA was standardized to 1 µg each sample. DNA was removed using Amplification-grade DNase I from Invitrogen. Ribosomal RNAs were reduced using the Ribo-Zero Gold rRNA Removal Kit from Illumina. Sequencing libraries were then prepared using the ScriptSeq v2 RNA-Seq Library Preparation Kit from Illumina. Barcodes used were Illumina TruSeq indexes 1-12.