title: GSM4459879: HHZ_root_rep3; Oryza sativa; RNA-Seq
library_construction_protocol: At 50 days after germination (DAG), plants were transferred to NH4NO3-free solution for 2 days. Then, plants were transferred back to normal culture solution (containing 1 mM NH4NO3) for 3 hours. Then roots were sampled and frozen in liquid nitrogen immediately. Three replications were used. We used the PureLink RNA Mini Kit (Life Technologies Corporation) for RNA extraction from roots. To do this, we firstly grinded the root sample with liquid nitrogen, then followed the manufacture's protocol for RNA extraction. RNA libraries were prepared for sequencing based on standard Illumina protocols.