title: GSM4384818: 4.5 dpf CHT cells; Danio rerio; RNA-Seq
library_construction_protocol: Then single cell suspensions (300-1,000 living cells per microliter determined by trypan blue staining) were loaded on a Chromium Single Cell Controller (10× Genomics) to generate single-cell gel beads in emulsion (GEMs) by using Single Cell 30 Library and Gel Bead Kit V2 (10× Genomics, 120237). Captured cells were lysed and the released RNAs were barcoded through reverse transcription in individual GEMs. Barcoded cDNAs were pooled and cleaned up using beads (Invitrogen, 37002D). 10X genomics scRNA-Seq: Single-cell RNA-sequencing (scRNA-seq) libraries were prepared using Single Cell 30 Library Gel Bead Kit V2 (10× Genomics, 120237) following the manufacture's introduction.