title: GSM3204636: bln1_t32_rep2; Hordeum vulgare; Blumeria graminis f. sp. hordei; miRNA-Seq
library_construction_protocol: Total RNA was isolated from 0.5 g of each pool using a hot (60˚C) phenol / guanidine thiocyanate method (Caldo et al., 2004. Plant Cell 16: 2514-2528. DOI: 10.1105/tpc.104.023382). Small RNA-sequencing (small RNA-seq) libraries were prepared by the Iowa State University DNA Facility (Ames, Iowa, USA) using the Illumina TruSeq small RNA library preparation kit and were subjected to single-end sequencing (100-bp reads) using the Illumina Hi-Seq 2500 Sequencing System. A split-split-plot design was used to run the 90 samples on three Hi-Seq 2500 flow cells. Each replication was run on a separate flow cell with each plant genotype randomly assigned to each lane and 6 barcodes randomly assigned to the 6 time points within each genotype (lane).