title: GSM3267392: Dif_NSCs__Cont.rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from cerebral cortical regions of brains or neuronal stem cells were extracted using Rneasy Plus Midi kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared according to Life Techonology's instructions accompanying the Ion AB Library Builder™ System and Library Builder. Briefly, total RNA was treated with Dynabeads® mRNA DIRECT™ Microkit to prepare PolyA-selected messenger RNA. RNA libraries were then prepared using the Life Techonology Ion Total RNA-Seq Kit for the AB Library Builder™ System and Library Builder. High-throughput sequencing was performed using the Ion Proton System. Sfpq mRNA level was down-regulated less than 10% in Neuro-2a treated with Sfpq-siRNA comparing those of Luc-siRNA.