title: GSM2967280: shP53_Ctrl-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIZOL and treated with DNase. RNA was used for reverse-transcription followed the SMART-seq2 protocol. Then cDNA was fragmented by sonication using the Covaris sonicator. Fragmented cDNA was end-repaired, ligated with A base. After A base addition, the pre-indexed Illumina adapters were ligated. cDNA with adapters were enriched for another 11 cycles. Finally, the acquired cDNA libraries were sequenced on HiSeq X Ten platform.