title: Illumina HiSeq 3000 sequencing; RNAseq of Atlantic salmon with CRISPR/Cas9-mediated mutation of delta-5 and multiple delta-6 fatty acyl desaturase genes
design: RNAseq of Atlantic salmon with CRISPR/Cas9-mediated mutation of delta-5 and multiple delta-6 fatty acyl desaturase genes
library_construction_protocol: Fish were anesthetized with Finquel MS-222 (ScanVacc) and then killed with a blow to the head. Liver was taken, flashed frozen in dry ice and stored in -80 before RNAseq. The fish had not been starved prior to sampling.  Total RNA was extracted with Qiagen RNeasy Plus Universal Kit. RNA quality was measured with Nanodrop8000 and Agilent Bioanalyzer 2100.   Library preperation was done with TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).