title: GSM3267378: LOS_#4 Kidney; Bos indicus x Bos taurus; miRNA-Seq
library_construction_protocol: Total RNA was isolated from frozen tissues (-80°C) using TRIzol™ Reagent (Invitrogen) following the manufacturer's instructions. RNA samples were stored at -80°C. RNA quality was determined by both spectrometry and fragment analysis. For each sample, 2 μg RNA was submitted to the University of Missouri DNA Core Facility for library construction. Libraries were prepared with TruSeq® Small RNA Library Prep Kit (Illumina) following the manufacturer's instructions. Size selection of 140-160 bp were performed on polyacrylamide gel.