title: GSM4586380: Dmel-ISO1-Illumina; Drosophila melanogaster; OTHER
library_construction_protocol: Whole bodies of 3-5 day old female virgin flies (ISO-1 strain, ~25 individuals) were collected and used for DNA extraction. DNA quality was assessed by OD260/OD280 with Nanodrop and agarose gel electrophoresis. The library for Illumina sequencing was prepared as follows: (1) Fragmentation with Covaris ultrasonicator, (2) End-repair and phosphorylation of the 5′ ends, (3) A-tailing of the 3′ ends, (4) Ligation of adapters, (5) PCR for several cycles to enrich for the ligated product. Sequencing was done with the Illumina HiSeq-2500 sequencer (run type: paired-end; read length: 125 nt) in Novogene (Tianjin, China).