title: FIL DSB REP2
design: Genomic DNA was isolated in agarose plugs as described (Schrader et al., 2005). DNA in agarose plugs was first blunted by End-It DNA Repair Kit (Epicentre) and then ligated to biotinylated linkers. DNA was then fragmented by sonication at low power for 7 cycles (30′′ on/30′′ off) in a Bioruptor (Diagenode) to yield a 500–1350 bp distribution of DNA fragments. Biotinylated fragmented DNA was isolated on streptavidin beads and ligated to Illumina linkers.