title: GSM4586418: RNA_U937_Ctrl_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from fresh U-937 cell pellets using Trizol reagent (Invitrogen). Total RNA was extracted using miRNeasy Micro Kit (Qiagen) following the manufacturer's protocol. 1 μg of the RNA samples were processed with the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat #. RS-122-2301), following the manufacturer's instructions. Cluster generation and sequencing took place on the Illumina NovaSeq 6000 using the reagents provided in the Illumina NovaSeq 6000 S1 Reagent Kit (200 cycle) with >30M paired-end 100 reads per sample