title: Complete genome sequencing of  Synsepalum dulcificum Fresh  leaf tissues
design: High Molecular  weight genomic DNA was extracted from the leaf material using a modified CTAB  extraction protocol . The genomic DNA was sheared to 20kb     and fragments shorter than 7 kb were filtered using BluePippin. The  filtered DNA was the nused    to prepare a proprietary SMRTbell library using the PacBio DNA    Template Preparation Kit .