title: GSM2443683: C32; mouse gut metagenome; OTHER
library_construction_protocol: Fecal and cecal samples collected at the time of harvest contents using QIAmp DNA stool Mini Kit (Qiagen, CA) with bead-beating. DNA concentration and quality was measured using Cytation5 (Biotek) The V3-V5 variable regions of the 16S rDNA were amplified using primers (forward F357: 5’-CCTACGGGAGGCAGCAG-3’ and reverse R926: 5’-CCGTCAATTCMTTTRAGT-3’)