title: Transcription profiling by RNA-seq
design: Transcription profiling by RNA-seq
library_construction_protocol: MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. MCF10A cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's directions.