title: GSM1832292: KO_Thy_Treg3; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were lysed with TRIzol and stored at -80 degree. Total RNA was extracted according to the manufacturer’s manual (Life Technologies). cDNA libraries were generated after SMART amplification (Clontech) and samples were individually indexed. Libraries were sequenced using a HiSeq 2000 platform (Illumina) according to a standard paired-end protocol.