title: GSM2190175: GFP-Normal rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from U2OS cells overexpressing actin mutant G13R and those expressing GFP as control, at both normal-growth and serum-stimulation conditions. Poly(A)+ mRNAs were enriched using Dynabeads oligo (dT) beads (NEB). RT reactions and purification of cDNA templates were performed following the RNA-seq sample preparation protocol from Illumina. Each cDNA sample was sequenced on an IlluminaHiseq 2500 system. RNA libraries were prepared for sequencing using standard Illumina protocols