title: GSM4761825: Fibroblasts_ChIP_H3K27AC; Homo sapiens; ChIP-Seq
library_construction_protocol: We used SimpleChIP enzymatic chromatin IP kit (Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer instructions with a slight modification. In brief, 4 million cells were cross-linked with 1% formaldehyde for 10 min at room temperature, and then quenched with 0.125 M glycine for another 10 min. The cells were washed with DPBS and resuspended sequentially in three lysis buffers according to the manufacturer's instructions. Chromatin was sonicated for 10–18 cycles (30 sec on/30 sec off) using Bioruptor Pico (Diagenode, Seraing, Belgium), and then incubated overnight at 4°C with histone and IgG control antibody. Immunoprecipitated chromatin was eluted by protein G magnetic beads followed by reversal of the cross-linking and DNA purification. The construction of library was performed using NEBNext®UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.