title: Illumina HiSeq 2000 paired end sequencing; An mRNA-sequencing atlas of mouse CD4+ T cell transcriptomes
design: An mRNA-sequencing atlas of mouse CD4+ T cell transcriptomes
library_construction_protocol: CD4+, CD62L+ cells were purified with CD4+CD62L+ T Cell Isolation Kit II (Miltenyi Biotec) and further purification was performed by FACS using anti-CD62L and anti-CD4 Fab fragments. Poly-(A)+ RNA was purified from ~500,000 cells using the Oligotex kit (Qiagen). The manufacturers protocol was modified slightly to include additional final elution steps resulting in a larger volume. RNA was purified, reverse transcribed and prepared for sequencing described in http://msb.embopress.org/content/7/1/497