title: GSM1831652: mTEClo_KO_Rep2; Mus musculus; RNA-Seq
library_construction_protocol: Thymi from fourteen 7-days old C57BL/6 mice were isolated and stromal cell enrichment was performed. Thymic stromal cells were stained with biotinylated Ulex Europaeus Lectin 1 (UEA1; Vector Laboratories), PE-Texas Red conjugated streptavidin (BD Biosciences) and the following antibodies from BD Biosciences: Alexa700 anti-CD45, APC-Cy7 anti-EpCAM and Alexa647 anti-Ly51. Both cTEC (CD45 EpCAM+Ly51+UEA1 ) and mTEC (CD45 EpCAM+Ly51 UEA1+) populations were sorted on a three laser FACSAria (BD Biosciences). Total RNA was isolated using Trizol reagent as recommended by the manifacturer (Invitrogen), and the further purified using RNeasy columns (Qiagen). Transcriptome libraries were generated from at least 10ng of total RNA using the TruSeq RNA Sample Prep Kit (v2) (Illumina). RNA libraries were prepared for sequencing following recommended Illumina protocols.