title: GSM2612289: ChGA_2_RNASeq; Mus musculus; RNA-Seq
library_construction_protocol: Intestinal epithelial cells from organoids were dissociated and immediately sorted by fluorescence-activated cell sorting. ~1% of total epithelial cells were GFP+ and collected. The remaining GFP- cells were retained for comparison. RNA from GFP+ and GFP- subgroups was then extracted and prepared for cDNA library generations using the SMARTer Ultra Low Input RNA kit. The Low Input Library Prep Kit (version 2, Clontech Laboratories, Inc.).  cDNA quality was assessed via bioanalyzer using the High Sensitivity DNA kit (Agilent Technologies), and high quality samples were preserved for sequencing