title: GSM4585281: Cur CC4; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: Total RNA was extracted with peqGOLD RNA kit (Peqlab; Erlangen, Germany) following the manufacturers' instructions. The genomic DNA was removed with one column RNAase-free with DNAase. The integrity of RNA was tested on a bleach agarose gel (Aranda et al., 2012) and for purity using NanoDrop OneC (Thermo Fisher Scientific, Braunschweig, Germany). For transcriptomic analysis, approximately 10 µg of total RNA was used for high-throughput cDNA sequencing by Illumina HiSeqTM 4000 technology (BGI Genomics Co.). RNAs derived from three biological replicates were used to generate the libraries. A total of eighteen libraries were constructed, from samples harvested seven days after the onset of treatments (control and +Fe). The cDNA libraries were prepared according to Illumina's protocols. The libraries were sequenced using filter.