title: GSM2027453: Tame_fox_prefrontal_cortex_ID190; Vulpes vulpes; RNA-Seq
library_construction_protocol: Total RNA samples were extracted from all 48 brain samples with Qiagen RNeasy Lipid Tissue Mini Kit (Qiagen, CA). QIAzol Lysis Reagent was used to remove excessive lipids in the brain tissue.  A260/A280 absorption ratios and RNA concentrations were measured by NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, DE). RNA-seq libraries were constructed from 1.5 µg total RNA using Illumina TruSeq RNA Sample Preparation Kits v2 according to manufacture protocols (Illumina Inc., CA), and sequenced on an Illumina HiSeq2000 instrument.