title: GSM2793593: 007B; Macaca mulatta; RNA-Seq
library_construction_protocol: Liver samples were homogenized in 1 mL TRIzol per 50 mg wet weight. Manual grinding with a pestle was used to pulverize the tissue, following which the sample was incubated for 5 min at room temperature. RNA extraction proceeded using a Direct-zol RNA kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions. RNA was then submitted to the University of Wisconsin-Madison Biotechnology Center Sequencing Facility for high throughput RNA-Seq. Each RNA library is generated following Illumina “TruSeq RNA Sample Preparation Guide and the Illumina TruSeq RNA Sample Preparation Kit (Illumina Inc., San Diego, California, USA). Quality and quantity of finished libraries are assessed using an Agilent DNA1000 series chip assay (Agilent Technologies, Santa Clara, CA) and Invitrogen Qubit HS Kit (Invitrogen, Carlsbad, California, USA), respectively. Each library is standardized to 2μM. Cluster generation is performed using a TruSeq Paired End Cluster Kit (v3) and the Illumina cBot, with libraries multiplexed for 2x100bp sequencing using the TruSeq 100bp SBS kit (v3) on an Illumina HiSeq2000. Images are analyzed using CASAVA 1.8.2.