title: GSM1468343: 4C.HOXA5-A.ESC.Δ5|6; Mus musculus; OTHER
library_construction_protocol: 10 million cells were trypsinized and crosslinked in 2% formaldehyde and 10% FBS in PBS for 10 min at room  temperature. The primary restriction enzyme used was DpnII. The secondary enzyme was Csp6I. Template DNA (1ug) was amplified using inverse primers containing barcoded illumina sequencing adapters on either end. Libraries were constructed by adding barcoded illumine adapters to the 5' end of each primer. PCR reactions were performed using the Expand Long Template PCR System (Roche), and 100-700bp DNA was gel purified and quantified before sequencing.