title: GSM1831809: KBM7_WT2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using TRIreagent (Sigma) RNA for total RNA-seq was depleted for Ribosomal RNA using the RiboZero rRNA removal kit (Human/Mouse/Rat) (Epicentre) according to manufacturer's protocol. Pretreeated RNA was then subject to library preparation. Strand-specific RNA-seq libraries were prepared employing the TruSeq RNA Sample Prep Kit v2 (Illumina) modified to obtain strand-specificity using dUTP incorporation and UDGase treatment. RNA-seq was performed by the Biomedical Sequencing Facility (BSF) in Vienna using the Illumina HiSeq 2000 platform.