title: GSM1471661: IMIM-PC2 WT Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from parental and MN58b-resistant cells was extracted and purified using Trizol, RNA integrity was assayed on an Agilent 2100 Bioanalyzer (range 8.4-10). PolyA+ fractions were purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq Stranded mRNA Sample Preparation Part # 15031047 Rev. D". Adapter- ligated library was completed by 10 cycles of PCR with Illumina PE primers. The resulting purified cDNA library of template molecules was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx (GAIIx) with SBS TruSeq v5 reagents following manufacturer's instructions (SingleRead 1x40 bases). Image analysis and per-cycle base calling was performed with Illumina Real Time Analysis software (RTA1.13 RNA libraries were prepared for sequencing using standard Illumina protocols