title: GSM4460492: ESC_ALL_S1_2to4C_NA_NA.mm10.RT; Mus musculus; OTHER
library_construction_protocol: Exponentially growing cells were cross-linked by adding fresh 1% paraformaldehyde solution to the ES media for 10 minutes at room temperature. The cells were washed twice with 1× phosphate-buffered saline and scraped off the plates, pelleted and nuclei were prepared as described in (Lam et al., 2019). Replicating cells were sorted based DNA content. Whole genome sequencing libraries were prepared from 100 ng of purified DNA using the KAPA Hyper Prep Kit (Cat. No. KK8502) following the manufacturer's instructions.