title: GSM3044558: SRSF2 P95H RNA-seq rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: HITS-CLIP: cells were UV-crosslinked (400mJ, Stratalinker 2400, Stratagene). RNA–protein complexes were immunoprecipitated with anti-Flag M2 Agarose beads (Sigma). RNA was partially digested with RNase A (Affymetrix) and P32-labeled (Perkin Elmer). SRSF2-RNA complexes were isolated by SDS-PAGE, treated with proteinase K, followed by RNA linker ligation.   RNA_seq: QIAGEN RNeasy minikit protocol HITS-CLIP: Ilumina small RNA library protocol for linker ligation.   RNA-seq: rRNA depletion