title: GSM1174826: Mid log_wildtype_glc minimal media + adenine_aerobic; Escherichia coli K-12; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Rneasy Mini Kit procedure with on column Dnase treatment (Qiagen). Paired-end, strand-specific RNAseq libraries were generated using the dUTP method {Levin JZ et al. 2010, Nat Methods} with the following modifications. rRNA was removed with Epicentre’s Ribo-Zero rRNA Removal Kit. Subtracted RNA was fragmented for 3 min using Ambion’s RNA Fragmentation Reagents. cDNA was generated using Invitrogen’s SuperScript III First-Strand Synthesis protocol with random hexamer priming.