title: GSM2443835: TA3 2i/Lif RNA-seq; Mus musculus; ssRNA-seq
library_construction_protocol: Undifferentiated iPSCs were sorted for SSEA1+ to control for the homogeneity of cells before RNA extraction using Dneasy Blood & Tissue Kit (Qiagen). Total RNA samples (RIN > 9.4, 1 ug) were subjected to synthesis of ribosomal RNA-depleted, directional Illumina NextSeq deep sequencing libraries using the TruSeq Stranded Total RNA LT with Ribo-Zero Gold Set A (Illumina Cat# RS-122-2301) following the protocol provided by the manufacturer (Illumina Cat# RS-122-9008DOC, Part# 15031060 Rev. E). Stranded (directional) RNA-seq. Paired-end (75 nt + 75 nt).