title: GSM4585312: CD34+, Endothelial Media Day9_Rep2; Homo sapiens; miRNA-Seq
library_construction_protocol: miRNAs on the respective days from ASCs were isolated using Purelink miRNA isolation kit (Thermofisher Scientific, K157001). The quality and purity of miRNAs was analyzed using fragment analyzer cDNA libraries were constructed using the Ion Total RNA-Seq Kit v2 (Catalog number: 4475936), protocol for the small RNA libraries. Templates for RNASeq were prepared with the Ion PI™ Hi-Q OT2 200 template Kit (A26434) using the Ion OneTouch™ 2 System for Ion Proton™ System semiconductor sequencing.