title: GSM4699125: RNAseq.F_KD_rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol (Ambion) from salivary glands (10-12 animals per sample is sufficient) vortexed at 4°C for 2 h, extracted with ethanol precipitation, subsequently purified with PureLink RNA Kit columns (Invitrogen). RNA-seq libraries were constructed using the dUTP method (Parkhomchuk et al., 2009).