title: Draft genome sequence of Klebsiella oxytoca Kd70 TUC-EEAOC isolated from the major destructive sugarcane pest in Argentina Diatraea saccharalis stalk borer
design: Klebsiella oxytoca Kd70 TUC-EEAOC was isolated from the intestines of D. saccharalis larvae collected from sugarcane plants, Tucuman, Argentina. DNA extraction was performed using the PureLink Genomic DNA Kit (Invitrogen, USA) according to the manufacturer’s instructions. The entire genome of the Kd70 strain was sequenced using GS-FLX using Titanium chemistry (454 Life Sciences, Roche Applied Sciences, INDEAR, Argentina). The 454 reads were assembled with Newbler 2.6 with an overall sequence coverage of 40X. Sequence annotation was performed using RAST Server software followed by manual inspection.