title: GSM3656742: WT4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from TA muscles using a miRNEasy kit (Qiagen, Valencia, CA, USA). RNA quality was assessed using a TapeStation (Agilent, Santa Clara, CA, USA) and all samples had a RNA integrity number greater than 9.0. For each sample, 250ng total RNA was delivered to the University of Michigan Sequencing Core for RNA sequencing (RNAseq) analysis. Sample concentrations were normalized, and cDNA pools were created for each sample, and then subsequently tagged with a barcoded oligo adapter to allow for sample specific resolution. RNA libraries were prepared for sequencing using standard Illumina protocols