title: GSM3044654: shRNAseq_pool2_LU_rep1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from harvested cells using DNAzol reagent (Invitrogen) following the suppliers manual. To retrieve shRNA sequences that have previously been integrated into the genomic host DNA via lentiviral transduction, custom PCR-primers were used that spcifically bind to pGIPZ vector sequences flanking the shRNA hairpin sequence. A PCR (24 cycles) was done for with the genomic DNA as template to amplify the shRNA sequences. PCR products were gel-purified and finally libraries were quantified and the size was determinded using Experion Automated Electrophoresis System (Bio-Rad).