title: GSM4585332: 13 days past RA injection SCARKO mutant (117698); Mus musculus; RNA-Seq
library_construction_protocol: Briefly, cells, barcoded microparticle beads and lysis buffer were co-flown into a microfluidic device and captured in nanoliter sized droplets. After droplet collection and breakage, the beads were washed, and cDNA synthesis occurred on the bead. Excess oligos were removed by exonuclease I digestion. cDNA amplification was done from a pool of 2,000 bead. Individual PCRs were purified and pooled for library generation. A total of 600 pg of amplified cDNA per sample was used for a Nextera XT library preparation (Illumina).