title: RNAseq of Gasterosteus aculeatus: adult muscle tissue 12C development rep6
design: DNase treated mRNA was purified using BIO-O NEXTflex  Poly-A beads. Sequencing libraries were prepared using the BIO-O NEXTflex  Rapid RNA-Seq kit. Each sample was individually barcoded and samples from different treatments were evenly distributed across 3 sequencing lanes of an Illumina HiSeq 2000 flow cell (10 samples/lane, two samples from each treatment/lane)