title: GSM2255651: MSC_WT_3; Mus musculus; RNA-Seq
library_construction_protocol: cDNA was synthesized, barcoded, pooled and pre-amplified according to Soumillon et al. 2014. 800 pg of the cDNA pool was used for library preparation using the Nextera XT Kit (Illumina) Lineage-negative, Sca-1+, Kit+ (LSK) and niche cells were FACS sorted according to Istvanffy et al. 2011. Cells were sorted into lysis buffer composed of 0.2 % Triton-X100 and 0.5 U/µl RNAse Inhibitor