title: GSM3656410: E14.5-16.5 OE-r1; Mus musculus; RNA-Seq
library_construction_protocol: E14.5 cortex was in utero electroporated with GFP plasmid (either CAG control or Zeb1-overexpression plasmid) and developed for 48h in utero. Then the cortices were dissected and dissociated with papain to single cells. GFP+ cells were sorted out. Total RNA was extracted with Trizol reagent (Life Technologies, USA, Cat.No.15596018) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using the standard BGISEQ-500 protocol.