title: GSM4459009: Healthy Donor 1 - 0hr - Tconvs; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using mirVana (Applied Biosystems) isolation kit. RNA-seq libraries were prepared from 4 μg of total RNA using the TruSeq RNA sample preparation kit following manufacturer's protocol (Illumina). Briefly, oligo-dT purified mRNA was fragmented and subjected to first and second strand cDNA synthesis. cDNA fragments were blunt-ended, ligated to Illumina adaptors, and PCR amplified to enrich for the fragments ligated to adaptors. The resulting cDNA libraries were verified and quantified on Agilent Bioanalyzer and RNA-seq was conducted using the GAIIx Genome Analyzer (Illumina).