title: GSM2445309: LUHMES_rep1; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Extracted and purified using the Qiagen Puregene kit WGBS libraries were prepared using 500 ng of DNA bisulfite converted using Zymo’s EZ DNA Methylation-Lightning kit according to the manufacturer’s instructions. Then, 100 ng of converted DNA was used to prepare the library using the EpiGnome/TruSeq DNA Methylation. The manufacturer’s instructions were followed except we performed 14 cycles of amplification instead of 10 in the last PCR step.