title: ILSC.NN
design: Dna was isolated from flash frozen fecal samples (Wang et al. 2009; PMCID: PMC2713796). The 16S rRNA gene V4-V5 region was amplified following EMP protocols  (http://www.earthmicrobiome.org/emp-standard-protocols/16s/). Paired-end sequencing was performed on an Illumina MiSeq sequencer. Fastq sequence data were demultiplexed in Qiime and split into per-sample files in Qiime.