title: RNA-Seq of human: fetal brain
design: ~5 ng of polyA+ was used as input for the TruSeq Stranded mRNA-seq kit (Illumina) with parameters set for ~150 bp insert-size libraries. Final purified libraries were assayed both by fluorometer (Qubit, ThermoFisher) for dsDNA concentration and Bioanalyzer (DNA12000 chip, Agilent) for size. Sequencing-by-synthesis (SBS) was performed on a HiSeq 2500 with 2x125 bp reads. Reads were demultiplexed using deML (Renaud et al. 2015)