title: amplicon data bacterial dog skin microbiome
design: paired end 16S rRNA gene amplicon sequencing  using Bateria primers  using nested PCR approach with 341F/1061R (V3-V6) (20 cycles) followed by a second PCR with primer system  515F-Y and 926R-jed (V4-V5) (20 cycles), Annealing Temperature 50C  were used