title: GSM4714563: ARP1_KD-SRD5A1+Dox; Homo sapiens; RNA-Seq
library_construction_protocol: ARP1 cells were pelleted and total RNAs was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were checked and ~10 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads. Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces and reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq™ 6000 following the vendor's recommended protocol. Illumina NovaSeq 6000 platform was utilized for PE150 sequencing
attributes.tag: GEO Accession
attributes.value: GSM4714563