title: GSM4714740: Ssrp1 Control rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with RNAiso Plus (Takara) and was subjected to DNaseI (Thermo Scientific) for 30min 1 μg total RNA with RIN value above 9 was used for following library preparation. Next generation sequencing library preparations were constructed according to the manufacturer's protocol. The rRNA was depleted from total RNA using mouse Ribo-Zero rRNA removal Kit (Illumina). The rRNA-depleted RNA was then fragmented and reverse-transcribed. First strand cDNA was synthesized using ProtoScript II Reverse Transcriptase with random primers and Actinomycin D. The second-strand cDNA was synthesized using Second Strand Synthesis Enzyme Mix. The double stranded cDNA were ligated with adapters, amplified by PCR and purified. libraries with different indices were multiplexed and loaded on an Illumina instrument according to manufacturer's instructions (Illumina) and pair-end sequenced for 10G data.
attributes.tag: GEO Accession
attributes.value: GSM4714740