title: Illumina HiSeq 1500 paired end sequencing; Galectin-3 interacts with components of the nuclear ribonucleoprotein complex
design: Galectin-3 interacts with components of the nuclear ribonucleoprotein complex
library_construction_protocol: Human cervix carcinoma cells (HeLa) were cultured in DMEM high glucose/ 10 % FCS, 2 mM glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin. Human kidney clear cell carcinoma cells (RCC-FG1) were cultured in Mc Coy s 5a/ 10 % FCS, 2 mM glutamin at 37 °C and high humidity. HeLa cells were transfected by electroporation with the Biorad Gene Pulser II. Up to 15 μg of siRNA were used for silencing of galectin-3 and/or galectin-1. For successful depletion the cells were transfected twice and harvested 48 h thereafter. HeLa cells were transfected with siRNA to silence galectin-3 and with the non-silencing control siRNA against firefly luciferase. Total RNA was isolated with the RNeasy Mini Kit (Qiagen). Libraries were prepared using the TruSeq® Stranded mRNA LT Kit according to maufacturer's instructions.
attributes.tag: Experimental Factor: siRNA Luciferase 3
attributes.value: RNA