title: DNase_hypersensitivity_Mature B cell ALL_
design: design_description
library_construction_protocol: Z138 cell line nuclei were isolated and briefly treated with different concentrations of DNaseI enzyme. Based on quality check 60U DNase treated sample was further processed with sucrose fractionation and purified sample was passed to the illumina library
attributes.tag: EXPERIMENT_TYPE
attributes.value: Chromatin Accessibility
attributes.tag: EXTRACTION_PROTOCOL
attributes.value: Nuclei were isolated from respective cells using Buffer A (15mM NaCl,60mM KCl,1mM EDTA, pH 8.0,0.5mM EGTA, pH 8.0,15mM Tris-HCl, pH 8.0,0.5mM Spermidine ) supplemented with 0.015% IGEPAL CA-630 detergent.
attributes.tag: DNASE_PROTOCOL
attributes.value: DNaseI treatment was done for 3 minutes and reaction was stopped with stop buffer ( 50mM Tris-HCl, pH 8, 100mM NaCl, 0.10% SDS, 100mM EDTA, pH 8.0, 1mM Spermidine, 0.3mM Spermine), sample was further fractionated on 9% Sucrose gradient for  24hrs/25000rpm at 16ºC.