title: The influence of diet on the grass carp intestinal bacteria and bile acids:                         Sample 821-10yp
design: The grass carp was fed Sudan grass (SG group) or formula feed (FF group) for about four month. At 21-Aug-2015, the hindgut content of 10 fish (SG1-5 and FF1-5) were collected and the DNA were extracted using the QIAamp DNA stool mini kit. PCR was performed with a barcode at the 5’-end of 515 F primary. The PCR products were purified and mixed for clone library construction, and the library was subjected to Illumina-based high-throughput sequencing.  