title: GSM2193085: C3, rep 1; Mycobacterium marinum; RNA-Seq
library_construction_protocol: Total RNA was extracted from 50 ml of M. marinum grown in Sauton's defined media using an RNeasy minikit (Qiagen) according to the manufacturer's recommendation with the following modifications. After bacterial cells were harvested, they were resuspended in 1 ml of RLT buffer containing 0.1% 2-mercaptoethanol and transferred to a 2-ml screw-cap tube containing glass beads. The mixture was homogenized in a mini-bead beater (BioSpec Products) with three 30-s pulses. RNA samples were treated with DNase I. cDNA libraries were constructed using the Illumina TruSeq RNA library preparation kit (v2), omitting the polyA selection step. Multiplexing sequences are included in the title of the associated fastq.gz files. After library quality control, sample libraries were pooled and sequenced in two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) in 50bp, single-end read format (SE50). After the sequencing run, reads were de-multiplexed and converted to FASTQ format using the Illumina bcl2fastq (v1.8.4) script.
attributes.tag: GEO Accession
attributes.value: GSM2193085