title: GSM2260098: GAL_BF3; Streptococcus pneumoniae; RNA-Seq
library_construction_protocol: Total RNA was extracted from each replicate separately using enzymatic lysis of pneumococcal cells (10 µL mutanolysin, 20 µL proteinase K, 15 µL lysozyme, and 55 µL TE) followed by RNA extraction with the RNeasy Micro Kit (Qiagen) with DNase treatment on column and in solution. Samples were then depleted of rRNAs using the Ribo-Zero rRNA Removal Kit for Gram-positive bacteria (Illumina, San Diego, CA). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2260098