title: Gen47Fungi
design: Genomic DNA were extracted from soil at the root zone.  Forward primer  name fITS7 Forward primer sequence (5' to 3') GTGARTCATCGAATCTTTG   Reverse primer name ITS4 Reverse primer sequence (5' to 3') TCCTCCGCTTATTGATATGC  were used. Illumina adapter overhang nucleotide sequences were added to the primer. The PCR reaction was performed.