title: GSM1485021: PH-ML ChIP-seq  (PH-ML_Chip_Rep1); Drosophila melanogaster; ChIP-Seq
library_construction_protocol: [ChIP-seq] Fixed cells were sonicated and PH-chromatin complexes were pulled down from clarified sonicated material Sequencing libraries were generated as described previously (Bowman et al., 2013). Briefly, ends of the immunoprecipetated DNA were repaired, followed by A-tailing, ligating to universal adapters, and amplification for 10 cycles with indexed primers. Excess adapters were removed by purification with Agencourt AMPureXP beads (Beckman Coulter). Fragment size was checked by Bioanalyzer using high sensitivity DNA chip, and an average size distribution of 300-500bp was observed. Libraries we multiplexed and sequenced on using Illumina HiSeq 2000 for 50bp single end reads. [RNA-seq] Total RNA was isolated using Qiagen RNA easy kit, ribosomal RNA was depleted by using ribozero gold kit and cDNA was prepared (and fragmented) by using Trueseq kit
attributes.tag: GEO Accession
attributes.value: GSM1485021