title: RNA-Seq of Dendrocalamus latiflorus: callus with adventurous shoots
design: Total RNA was extracted from samples using RNeasy Plant Mini Kit (Qiagen, China), as instructed by manufacture. RNA concentrations and integrity were determined using Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and then were used for constructing mRNA sequencing libraries. The cDNA was synthesized from the mRNA that was purified from total RNA, and then processing with end repair, adding dA and adapters. The cDNA products were further amplified by PCR using adapter primer sequences to generate the libraries for sequencing. The quality and quantity of each library was assayed in quantification and qualification by using ABI StepOnePlus Real-Time PCR System