title: GSM4649108: ACO_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) was performed in secretory cells and alveolar type 2 cells isolated from Scgb1a1-CreTM;R26RfGFP;Sftpc-dsRedIRES-DTR mice. Briefly, tagmentation reaction was performed with 50ng of target DNA, and then bPCR-compatible sites and barcode were added into the target DNA by PCR. After cleaning up the PCR products using Zymo DNA clean and concentrator-5 kit, the final product was sequenced using Illumina HiSeq 400 machine.  Global gene expression profiles using RNA-seq were performed in feeder-free cultured organoids derived from secretory cells and alveolar type 2 cells.  Briefly, total RNAs were isolated using the RNeasy plus minikit (Qiagen). mRNAs were enriched from total RNAs, fragmented, and primed. First strand cDNAs were synthesized following by second strand synthesis. The ends of purified double strand cDNAs were repaired, ligated with a barcoded adaptor, and amplified with PCR. Purified final PCR product was sequenced using Illumina NextSeq 500 machine. ATAC-seq library was constructed using Nextera DNA library prep kit following the manufacturer's protocol. RNA-seq library was constructed using NEBNext Ultra RNA Library prep kit (NEB, E7530S) according to manufacturer's instruction.
attributes.tag: GEO Accession
attributes.value: GSM4649108