title: RNA-seq Daphnia magna WTII
design: RNA extraction: RNeasy Mini Kit (Qiagen) + Desoxyribonuclease I (Fermentas) Libraries were prepared using the Illumina® TruSeq® RNA Sample Preparation Kit.  Library Preparation was started with 3µg total RNA. After poly-A selection (using poly-T  oligo-attached magnetic beads),  mRNA was purified and fragmented using divalent cations  under elevated temperature. The RNA fragments underwent reverse transcription using random  primers. This is followed by second strand cDNA synthesis with DNA Polymerase I and  RNase H. After end repair and A-tailing indexing adapters were ligated. The products were  then purified and amplified  (15 PCR cycles) to create the final cDNA libraries.  After Library validation and quantification (Agilent 2100 Bioanalyzer), equimolar amounts of  Library were pooled together. The pools were sequenced on an Illumina GAIIx sequencer with  a 2x36bp Paired-End protocol.