title: GSM4763887: MCF7 DMSO; Homo sapiens; ChIP-Seq
library_construction_protocol: Nuclear isolation and in situ Hi-C contact generation were performed by proximity ligation on 5x10^6 crosslinked cells per condition using biotin-dATP. The isolated nuclei were then lysed and sonicated prior to chromatin immunoprecipitation against the epitope of interest (H3K27ac, Diagenode Cat# C15410196, Lot# A1723-0041D). Next, reverse crosslinking and DNA purification were performed on the immunoprecipitated DNA prior to biotin capture of the Hi-C contacts. The HiChIP libraries were prepared as previously described (Mumbach et al Nature methods 2016), which consists of on-bead library prep with Illumina transposase (Tn5), adjusting enzyme amount linearly according to the amount of post-ChIP DNA (i.e. 1.25ul Tn5 for 25ng of DNA).
attributes.tag: GEO Accession
attributes.value: GSM4763887