title: Illumina HiSeq 2000 sequencing; qiita_ptid_792:833.California.HC27
design: Fecal samples from dogs of various breeds, places of origin, and severity of bowel disorder were sequencing to obtain a dog gut metagenome.
library_construction_protocol: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: barcode
attributes.value: GCTCTCCGTAGA
attributes.tag: center_name
attributes.value: CCME
attributes.tag: dna_extracted
attributes.value: True
attributes.tag: experiment_center
attributes.value: Texas A&M
attributes.tag: experiment_title
attributes.value: suchodolski_dog_ibd
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: physical_specimen_remaining
attributes.value: False
attributes.tag: platformchemistry
attributes.value: HiSeq_V4
attributes.tag: pool_name
attributes.value: R.K.1.20.12
attributes.tag: primer
attributes.value: GTGCCAGCMGCCGCGGTAA
attributes.tag: primer_plate
attributes.value: 2
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 1/30/12
attributes.tag: run_prefix
attributes.value: Suchodolski_dog_ibd
attributes.tag: samp_size
attributes.value: 0.1, gram
attributes.tag: sample_center
attributes.value: Texas A&M
attributes.tag: sample_plate
attributes.value: IBD2
attributes.tag: sample_type
attributes.value: XXQIITAXX
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: study_center
attributes.value: Texas A&M
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: well_id
attributes.value: C7