title: AccuNGS sequencing of HIV6 sample (gag amplicon)
design: Random hexamers priming; gag PCR amplicon, 40 PCR rounds with Superfi Platinum polymerase; PCR product extraction from agarose gel; Nextera XT library preparation kit with 12 PCR rounds using Superfi Platinum polymerase; library purification and size selection with AMPure XP beads; paired-end sequencing with Illumina MiSeq