title: GSM3578971: MED12 rep 3B RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from cultured cells was extracted using TRIzol reagent (Invitrogen). Strand-specific libraries were generated using the TruSeq PolyA Stranded mRNA sample preparation kit (iIlumina). In brief, polyadenylated RNA was purified using oligo-dT beads. Following purification, the RNA was fragmented, random-primed and reserve transcribed using SuperScript II Reverse Transcriptase (Invitrogen). The generated cDNA was 3' end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM3578971