title: Amplicon of E. muelleri microbiome: Twitchell Brook (hatched gemules)
design: Amplification of V4 hyper-variable region of the 16S rRNA gene with primers 515F-Y (Parada et al. 2016d) and 806R (Apprill et al.2015). PCR products were purified with AgencourtAMPure XP Beads, and libraries prepared with Nextera XT DNA Library Preparation Kit. Sequening was performed at the Natural History Museum of London.