title: GSM4471503: 5A_Z1365: Htt+/+ Hdac2+/+  rep9; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol/chloroform extraction. Briefly, pelleted cells were resuspended in Trizol reagent and then extracted with chloroform, followed by isoproponal precipitation of RNA from the aqueous phase and three 70% ethanol washes. RNA pellets were solubilized in 30-50 micro liter of RNase-free water. RNA quality was assessed using the Agilent Bioanalyzer TapeStation 2200 . RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Sample PrepKit. Each library also included 1 ul of a 1:10 dilution of ERCC RNA Control Spike-Ins (Ambion) that were added from one of two mixes, each containing the same 92 synthetic RNA standards of known concentration and sequence.
attributes.tag: GEO Accession
attributes.value: GSM4471503