title: freshwater metagenome
design: Effluent was collected from Sheboygan wastewater treatment plant, and was filtered through ~1.6 _m pore size glass fiber filter (pre-filtration) for collecting larger and particle-associated microorganisms. DNA was extracted using phenol:chloroform based extraction, and submitted for WGS sequencing with paired-end format (100bp each) and approximate insert size of 400bp.