title: Rainbow Trout Embryo Transcriptome, Skamania Clonal Line
design: Total RNA was isolated from Skamania embryos after yolk removal using a TRIzol extraction.  cDNA was synthesized using the SMARTer PCR cDNA synthesis protocol (Clontech) and then amplified using an Advantage PCR kit.  Purified (phenol-chloroform extraction) cDNA was then sequenced on a Roche 454 FLX Titanium Genome Sequencer.