title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells
design: RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells
library_construction_protocol: Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA.
attributes.tag: Experimental Factor: RNA interference
attributes.value: p150 siRNA