title: GSM1477433: E13.5_Male_PGC_KO_H3K27me3; Mus musculus; ChIP-Seq
library_construction_protocol: Primordial germ cells (SSEA1+) and Somatic gonadal cells (SSEA-) were isolated from the gonads of E13.5 embryos and purified by FACS. Cells were sorted directly in nuclear isolation buffer containing RNAse inhibitor and protease inhibitor cocktail. For RNA-seq libraries, RNA was extracted using TriReagent, and dsCDNA was synthesized using Superscript III and Klenow fragment, respectively. For ChIP-seq libraries, DNA was extracted from immunoprecipitated DNA-protein complexes using phenol:chloroform:isoamyl alcohol 25:24:1. Adaptor ligation based library construction for paired-end Illumina sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1477433