title: GSM2194371: ACAP236 22262; Homo sapiens; RNA-Seq
library_construction_protocol: Islets were dissociated to single cell by 0.05% Trypsin, 9 mins at 37oC. Cells (250-500 cells/μl) were subsequently loaded on Fluidigm C1 IFC with either 5-10 μM or 10-17 μM capture size. Smarter-seq V2 kit was utilized for library construction per manufacturer's protocol to generate cDNA.  Prepration of Illumina libraries from cDNA was done with the NexteraXT kit per Fluidigm’s instructions.
attributes.tag: GEO Accession
attributes.value: GSM2194371