title: GSM4763783: RNA-Seq OV 1; Brassica napus; RNA-Seq
library_construction_protocol: DNA was isolated from seeds using the Qiagen Plant DNeasy Mini Kit. RNA was isolated from seeds using the Qiagen miRNeasy mini kit. WGBS: Library preparation for bisulfite sequencing was performed at Genome Quebec. DNA was sheared via sonication on the Covaris platform prior adaptor ligation and bisulfite conversion using the EZ-96 DNA Methylation-Lightning Kit (Zymo®). Libraries were then amplified using the DNA UltraII Kit (NEB®). sRNAseq: Total RNA collected from seeds was used for library prep with the NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex compatible).
attributes.tag: GEO Accession
attributes.value: GSM4763783