title: GSM3405798: SMMC7721-OE+Nx; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed three times with PBS before RNA was harvested using Trizol reagent. ΒioTeke RNA extraction Kit  (Cat#RP4002) was used with 1 ug of total RNA for the construction of sequencing libraries. According to the experimental instructions, the purified total RNA was subjected to mRNA isolation, fragmentation, first strand cDNA synthesis, second strand cDNA synthesis, terminal repair, 3' end plus A, ligation linker, enrichment and other steps to complete the library construction of the samples.
attributes.tag: GEO Accession
attributes.value: GSM3405798