title: 16S rRNA gene tags sequencing of the baseline fecal microbiota - subject C
design: A double-blind, placebo-controlled, human crossover study with 24 healthy human subjects (22 and 38 years of age) was conducted with the primary objectives to determine acceptability and persistence of B. longum subsp. longum AH1206 at a daily dose of 10^10 cells. The study was conducted over two 7-week test periods. Each period included a baseline, a 14-day treatment and a 28-day test of persistence. Fecal samples sequenced were collected at baseline ( pre-AH1206 treatment), last day of AH1206 consumption (14 days) and last day of placebo consumption (14 days). The V5-V6 region of the 16S rRNA gene was amplified by PCR with primers F (5'-CGACRRCCATGCANCACCT-3') and R (5'-RGGATTAGATACCC-3')