title: GSM4152128: VCaP: Pol2_siCtrl_Ctrl2; Homo sapiens; ChIP-Seq
library_construction_protocol: VCaP cells were grown in DMEM medium supplemented with 10% feta bovine serum and 1% penicillin/streptomycin antibiotics at 37 degree C temperature incubator with 5% CO2. Nuclei from approximately 10 million formaldehyde crosslinked (1%; 10min at room temperature) cells were isolated and chromatin was fragmented using sonicator (bioruptor). Lysates were cleared and protein-DNA complexes were isolated using target specific antibodies and protein-A/G magnetic beads. DNA fragments were purified and libraries were prepared accorcing to standard Illumina protocol.
attributes.tag: GEO Accession
attributes.value: GSM4152128