title: HiSeq X Ten paired end sequencing; Identifying crossover locations in Arabidopsis thaliana wild type Col-0 x Ct-1 F2 populations using genotyping-by-sequencing
design: Identifying crossover locations in Arabidopsis thaliana wild type Col-0 x Ct-1 F2 populations using genotyping-by-sequencing
library_construction_protocol: Arabidopsis thaliana rosette leaves were collected from 5-week-old plants and frozen at -20°C. Arabidopsis thaliana plants were grown under long day 16/8 hour light/dark photoperiods at 20°C, 60% humidity and 150 μmol/m2/s light intensity. DNA was extracted from rosette leaves using CTAB. Sequencing libraries were constructed as previously described (Hennig et al, 2018 G3).
attributes.tag: Experimental Factor: individual
attributes.value: wild-type_186