title: GBS Data of R. canescens
design: DNA samples (250 ng) were digested with Msp1 and Pst1 at 37oC for 2 hours in a 96-well plate. Barcoded PstI adapters and MspI Y-adapters were ligated to the digested DNA fragments. PCR was conducted with each sample individually using a barcode-specific forward primer and a common adapter-specific reverse primer. The PCR products of the 96 samples were pooled (5 ng each).  Single-end sequencing of the GBS library was conducted using Illumina Nextseq 500 mid output flow cell.