title: Transcriptome sequence, assembly and annotation of Wyeomyia smithii
design: 12 samples of 30 larvae each of Wyeomyia smithii.  The 12 samples represented diapausing larvae, diapausing larvae on short days (L:D = 8:16), diapausing larvae exposed to 10 diapause-terminating long days (L:D = 18:6), pupae on long days and adults on long days.  Each stage of development was sampled at three times of day.