title: comparative analysis of bacteria and fungus communities on human scalp by metagenome
design: Our skin plays an important role as defense from a variety of factor correlated to human skin disease and it has many microorganisms including bacteria, fungi and virus. Several human skin diseases such as atopic dermatitis, seborrheic dermatitis and dandruff have been associated with microorganisms.  In order to investigate bacterial and fungal community, we collected samples from normal scalp and dandruff patient. From collected samples, we amplified a highly conserved region of the 16S rRNA gene about bacteria and D1/D2 region of the 26S rRNA gene about fungus using target primer. Using GS-FLX pyrosequencing, a number of 16S rRNA gene were read 79,119 sequences and 26S rRNA gene were read 42,849 sequences. Interestingly, Firmicutes (Staphylococcus spp.) were dominant in the dandruff scalp. In contrast, the most abundant potentially fungus in the healthy scalp was affiliated with the Basidiomycota (Malassesiza spp.) In conclusion, we found new information through comparative of bacterial and fungal communities in normal and dandruff-associated scalps.