title: GSM4100874: D4: pure pancreatic macrophages (CD45+CD11b+F4/80+) from mice in RAP on d4; Mus musculus; RNA-Seq
library_construction_protocol: Pancreases under RAP were removed. Macropaheges were isolated and sorted by FACSAriaⅡ to get pure macrophages (CD45+CD11b+F4/80+) and RNA was harvested using Trizol reagent. A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
attributes.tag: GEO Accession
attributes.value: GSM4100874