title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells
design: RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells
library_construction_protocol: ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA. Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina).
attributes.tag: Experimental Factor: RNA interference
attributes.value: p60 siRNA
attributes.tag: LIBRARY_STRAND
attributes.value: not applicable