title: GSM2870519: Subject10_milk; Homo sapiens; miRNA-Seq
library_construction_protocol: Small RNAs were extracted using the miRNeasy Serum/Plasma kit (Qiagen, Cat No.: 217184) with minor modifications. RNA extraction was performed from at least 3 mL of plasma and milk. One mL of biofluid was filtered per each column and washed two times with ethanol 80% before elution Libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (NEB, Cat No.: E7330) following manufactures instructions and using 150ng of RNA obtained from plasma samples and 500 ng from milk. Library size selection was done using acrylamide gels.
attributes.tag: GEO Accession
attributes.value: GSM2870519