title: Amplicon_16S
design: Libraries were prepared and sequenced by the Georgia Genomics and Bioinformatics Core at the University of Georgia. Libraries were constructed to capture the V4-V5 region of the 16S rRNA using primers from Parada et al. 2016: forward (5-GTGYCAGCMGCCGCGGTAA-3) and reverse (5-CCGYCAATTYMTTTRAGTTT-3). Amplicon libraries were prepared using an input of 2-5 ng/ul of DNA and sequenced using the Illumina MiSeq platform to produce 250+250 nt paired reads.