title: Illumina HiSeq 2500 sequencing; Small RNA profiling of sheep ICLN from MAP infected and uninfected animals
design: Small RNA profiling of sheep ICLN from MAP infected and uninfected animals
library_construction_protocol: At post-mortem the ileo-caecal lymph node (ICLN) from either diseased sheep with Paratuberculosis or uninfected control sheep were removed. The ICLN was treated with RNAlater RNA Stabilization Solution (Ambion) and stored at -80C prior to RNA extraction (according to manufacturers instructions). TruSeq Small RNA Library Prep Kit was used to prepare libraries.
attributes.tag: Experimental Factor: infect
attributes.value: Mycobacterium avium subsp. paratuberculosis
attributes.tag: Experimental Factor: disease
attributes.value: multibacillary paratuberculosis