title: GSM1560316: kidney-2; Scophthalmus maximus; RNA-Seq
library_construction_protocol: Samples from the three organs (head kidney, spleen and pyloric caeca) were collected in cold RNAlater (Qiagen), kept at 4ºC overnight and then transferred to -20ºC. RNA extraction was performed using the RNeasy mini kit (Qiagen) with DNase treatment following manufacturer’s instructions. RNA quality and quantity were evaluated in a Bioanalyzer (Bonsai Technologies) and in a NanoDrop® ND-1000 spectrophotometer (NanoDrop® Technologies Inc), respectively. The 12 samples were barcoded and prepared for sequencing by the Wellcome Trust Centre for Human Genetics, Oxford, using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM1560316