title: WGS water-column Oct,2013 (before rain): replicate 1
design: Water was collected from near the surface (0-1m depth) from North Shore Channel, and passed on-site in succession through ~1.6 _m pore size glass fiber filter for pre-filtration and captured on a 0.22 _m pore size polycarbonate membrane filter for collecting free-living prokaryotic component. DNA was extracted using phenol:chloroform based extraction, and submitted for WGS sequencing with paired-end format (150bp each) and approximate insert size of 600bp. This library represents one of the sequencing duplicates for a single biological sample (DNA) for this time-point.