title: GSM1838576: K562, siRNA aCP1/2 combined knockdown, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: K562 cells were harvested 72 hours after the siRNA transfection and RNAs were purified using Absolutely RNA miniprep Kit (Stratagene) according to manufacturer’s instruction. Libraries for RNA-seq analysis were constructed using the TruSeq RNA Sample Preparation Kit V1 according to manufacturer’s instruction. The libraries corresponded to the 3 control siRNA depletions  and 3 aCP1/2 co-depletions. These libraries were multiplexed and sequenced on a single channel on the Hiseq-2000 platform using a 100nt paired-end sequencing protocol by NGSC at University of Pennsylvania.
attributes.tag: GEO Accession
attributes.value: GSM1838576