title: Lactobacillus salivarius Transcriptome Sequencing
design: Fragmentation of rRNA depleted mRNA samples and subsequent preparation of cDNA libraries were performed using Illumina TruSeq Stranded mRNA Library Preparation kit (Low Sample Protocol), following manufacturers instructions. First cDNA strand synthesis in the reverse transcription polymerase chain reaction (RT-PCR) was performed using the reagents supplied by the manufacturer along with the SuperScript II reverse transcriptase (Thermo Fisher Scientific).