title: GSM4704130: Blank_CUT and Tag; Mus musculus; OTHER
library_construction_protocol: NovoNGS® ConA Beads were pipetted into a new tube, 10 μl per sample, and washed by ConA Binding Buffer. 1×105 cells on d4 were harvested and washed by Wash buffer. The cells were mixed with ConA Beads and incubated with Monoclonal ANTI-FLAG® M2 antibody (Sigma) (1:50) or without primary antibody (Blank) overnight at 4°C. Goat anti-mouse IgG antibody (Kitgen) was diluted at 1:500 and added into the sample, then the sample was incubated for 1 hr at RT. After washing away the unbounded secondary antibody, NovoNGS® ChiTag™ 2.0 Transposome was added and incubated with cells for 1 hr at RT. The sample was washed by ChiTag Buffer, and followed with tagmentation by Tagmentation Buffer. Next, DNA was isolated by NovoNGS® DNA Clean Beads, and dissolved in TE-RA-Buffer. DNA was amplified with N5 and N7 primers and purified with NovoNGS® DNA Clean Beads for high-throughput sequencing. CUT and Tag
attributes.tag: GEO Accession
attributes.value: GSM4704130