title: 16S rRNA sequence: Antarctic Marine Sediment
design: Microbial 16S rRNA gene fragments were amplified using a set of primers designed by adding a 10- nucleotide barcode to the forward primer, 519F, (5’-CAGCMGCCGCGGTAATWC- 3’) and reverse primer 1068R (5’-CTGACGRCRGCCATGC- 3’) (Wang and Qian, 2009). The amplification reaction was carried out using the Accuprime pfx SuperMix (Thermo Scientific) according to the manufacture. PCR was performed with a thermal cycler (Thermo Scientific) under the following conditions: 95°C for 5 min, 26 cycles of 95°C for 15 s, 59° C for 30 s and 68°C for 1 min. The PCR products were purified by using a DNA clean &amp; concentrator kit (Zymo Research).