title: RNA-seq of Cornus florida Little Princess isolate D14 at time point 3
design: Using a modified Zymo Direct-zol RNA protocol (Cat#: R2052) RNA was isolated from flowering dogwood (Cornus florida) leaves which were inoculated with powdery mildew (Erysiphe pulchra) followed by clean-up using Zymo Clean and concentrator kit (Cat#: R1017). Following isolation, RNA-seq libraries were prepared using the KAPA Stranded mRNA-Seq kit (library selection: poly-A). Sequences were generated at the University of Georgia's Georgia Genomics and Bioinformatics Core (GGBC) on the Illumina NextSeq500 sequencing platform.