title: GSM1198172: Egr2CKO CD4+ T 0h RNASeq; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-Seq, total RNA was extracted from 5x10^6 cells per sample using Rneasy Mini kit (QIAGEN) and the quality and concentration were determined by Nanodrop ND-1000 spectrophotometer. Double-stranded cDNA was synthesized using random hexamer primers, SuperScript II, DNA polymerase I, and T4 DNA polymerase (all from Invitrogen) and fragmented using Bioraptor. After repairing ends, adaptor (Illumina) was added using T4 DNA ligase (New England Biolabs), and 250-450 bp fragments were isolated using 2% E-Gel (Invitrogen) and amplified for 17 cycles using PE 1.0 and PE 2.0 primers (Illumina) and Phusion High Fidelity PCR Master Mix (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM1198172