title: GSM3579336: POL2noDOX6_S2.merged.bam; Homo sapiens; ChIP-Seq
library_construction_protocol: Crosslink was performed with disuccinimidyl glutarate (DSG) (Thermo Scientifis) for 45 minutes followed by 30 minutes incubation with formaldehyde 1%. The reaction was blocked with glycine 100 mM. Shearing was performed using Covaris S2 (Covaris, Woburn, MA) for 8 min at maximum intensity. The sonicated chromatin was incubated O/N at 4°C in presence of antibodies as described in (https://elifesciences.org/articles/27706) coupled to A/G sepharose beads (Santa Cruz Biotechnology). Kapa Hyper Prep Kit (Kapa Biosystems, Wilmington, MA) was used for End-repair, A-tailing and ligation of sequence adaptors. Samples were amplified by PCR and the libraries were size-selected in the 200-500 bp range.
attributes.tag: GEO Accession
attributes.value: GSM3579336