title: MiSeq of environmental DNA: activated sludge
design: isolated DNA samples were subjected to PCR amplification of the V4 variable region of 16S rRNA genes with primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACVSGGGTATCTAAT-3), and  bTEFAP Illumina MiSeq 2  300 bp barcoded amplicon sequencing.