title: GSM2193136: TBD-623; Prunus persica; miRNA-Seq
library_construction_protocol: Total RNA was extracted from the fruit samples using Trizol reagent and subsequently treated with DNaseI (Takara Biotechnology Co., Ltd, Dalian, China) per the manufacturer’s instructions to remove contamination by genomic DNA. sRNA fractions of 18 to 30 nt in length were first purified from the low-molecular weight RNA by excision from 15% denaturing polyacrylamide gels and then ligated to 3’ and 5’ adaptors using T4 RNA ligase according to the manufacturer’s instructions. The sRNAs ligated with 3’ and 5’ adaptors were purified and reverse transcribed to cDNA with the reverse transcription primers for 17 cycles according to the protocol. Eventually, the PCR products were further purified and subjected to sequencing using the Illumina Genome Analyzer. The image files obtained from the analyzer were then evaluated and used to form a large repertoire of digital data suitable for bioinformatics analysis. Degradome library was constructed following with methods previously described by Ma et al.(2010).  CleaveLand3.0  program was used to detect potentially sliced targets of known and novel miRNA.
attributes.tag: GEO Accession
attributes.value: GSM2193136