title: GSM4151673: M0_ATF4_ChIP_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin-IP was performed according to Cell Signaling ChIP kit # 9003 protocol Libraries were prepared according to NEBNext® Ultra™ II DNA Library Prep Kit for Illumina kit instructions (NEB #E7103). Briefly, DNA was end-repaired, using NEBNext Ultrea II end prep Enzyme mix. The blunt, phosphorylated ends were treated with fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were size selected using Ampure AMPure XP. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on Illumina HiSeq/NextSeq.
attributes.tag: GEO Accession
attributes.value: GSM4151673