title: GSM2194100: Lewis lung carcinoma (LLC) Brain metastatic derivative, replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using PrepEase RNA isolation kits (Affymetrix) according to the manufacturer's instructions. Extracted total RNA samples were quantified by Ribogreen and quality assessed by Agilent BioAnalyzer 2000. 500ng RNA with integrity number (RIN) > 9.5 from each sample was used for library construction with TruSeq RNA Sample Prep Kit v2 (Illumina) according to manufacturer’s instructions. Libraries were constructed for RNA-Seq analysis on Hi-Seq 2000 platform. HCC1954, PC9 and LLC: 50/50bp paired-end RNA-Seq; MDA-MB-231: 75/75bp paired-end RNA-Seq. 25-65 million (average 40 million) raw read pairs were generated for each sample.
attributes.tag: GEO Accession
attributes.value: GSM2194100