title: GSM4254410: WT-P0B; Mus musculus; RNA-Seq
library_construction_protocol: The wild-type (Wt) and Fyco1-/- mice at P0 were first anesthetized with isoflurane and subsequently euthanized through cervical dislocation, and the lenses were isolated using forceps under a microscope. Briefly, three biological replicates, each consisting of ten lenses for Wt and Fyco1-/- mice were used for RNA-Seq library preparation. Upon extraction, lenses were dissolved immediately in Trizol reagent and total RNA was extracted for RNA-Seq library prepration. NEB Next Ultra RNA Library Prep Kit was used to prepare RNA-Seq libraries using total RNA isolated from Wt and Fyco1-/- mouse lenses at P0.
attributes.tag: GEO Accession
attributes.value: GSM4254410