title: Late S phase DNA synthesis of U2OS cells 16 h after release with RO3306.
design: Late S phase DNA synthesis. Thymidine-synchronized U2OS were released for 16 h in warm medium with RO3306 (9M). EdU (25 M) was added to the medium 30 min prior to harvesting and fixing the cells. The EdU-labelled DNA was isolated and sequenced.