title: EdUseq-HU of U2OS cells transfected with RIF1 siRNA: 14 h after mitotic shake-off sequencing 2).
design: EdUseq HU 14 h after mitotic shake-off. U2OS cells transfected with RIF1 siRNA were synchronized by mitotic shake-off and released into media containing HU and EdU for 14 h before being collected. The EdU labeled DNA was then isolated and sequenced.