title: 16S rRNA amplicon sequence - soil microbial community
design: DNA was extracted from portions of 250 mg of homogenized ground soil using the MoBio PowerSoil DNA Isolation Kit.Extracted DNA was amplified using the 16S rRNA gene primer pair 515-F (5-GTGCCAGCMGCCGCGGTAA-3) and 806-R (5-GGACTACHVGGGTWTCTAAT-3), which included Illumina adapters and unique barcode sequences for each sample.