title: Cells sorted Vim+ at Day21 (1)
design: Amplicon sequencing results of regions containing gRNAs amplified from transduced cells with CRISPR-Cas9 library containing 1649 gRNAs. Two million cells were collected at the appropriated day of time point. DNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen). PCR products (DNA) of the regions of interest were used for library preparation. Libraries were prepared using Nextera DNA Flex library Prep (Illumina) and sequenced on a MISEQ platform (Illumina).