title: RNA-Seq of HCT116
design: For cDNA library preparation, we used RNA isolated from cell cultures with RIN (RNA integrity number) values  8. Libraries were prepared with TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) according to the manufacturer's recommendations. Transcriptome sequencing was performed on a NextSeq 500 System (Illumina) using 76 base pair single-end reads. At least 30M reads were obtained for each samples. Primary analysis of raw sequences was done as described in [21]. Then, data as transcripts per million (TPM) were imported in the R environment. Differential gene expression was analyzed using DESeq2 package of [22]. KEGG database was used for the pathway enrichment analysis.