title: GSM1194021: Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 2, technical replicate 2; Gallus gallus; RNA-Seq
library_construction_protocol: Total RNA was extracted using a RNeasy Midi kit according to the manufacturer’s protocol (Qiagen; Valencia, CA). The RNA concentration was determined with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies; Wilmington, DE). RNA integrity was examined using a RNA 6000 Nano Assay kit and the Model 2100 Bioanalyzer (Agilent Technologies; Palo Alto, CA). Paired-end sequencing libraries were made from 2 µg of total adipose RNA with the Illumina TruSeq® Stranded mRNA Sample Preparation Kit following standard Illumina protocols. Individual RNA samples were indexed (bar-coded) after the fragmentation step to enable multiplexing of libraries within sequencing lanes. Bar-coded libraries were pooled and sequenced using an Illumina HiSeq 2000 Sequencing System (http://www.illumina.com/systems/hiseq_2000.ilmn, Illumina, InC., San Diego, CA) at Delaware Biotechnology Institute, University of Delaware. All 8 libraries (4 HG and 4 LG) were multiplexed together and sequenced in two replicate lanes.
attributes.tag: GEO Accession
attributes.value: GSM1194021