title: RNA-Seq of root of Camellia oleifera
design: Total RNA was extracted using the Trizol reagent (Invitrogen, CA, USA) according to the manufacturers instructions. The quantity and purity of the total RNA were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 as the criterion. One RNA sample was collected for each of the 12 tissue samples. RNA-Seq of every sample was conducted separately to yield a gene expression profile. Approximately 10 g of total RNA was used to isolate poly-A mRNA with poly-T oligo-attached magnetic beads (Invitrogen, USA) for purification. Subsequently, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library following the protocol of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA); the average insert size for the paired-end libraries was 300 bp (50 bp). Finally, paired-end sequencing was performed on an Illumina HiSeq 4000 at Lc-bio (China) in accordance with the vendors recommended protocol.