title: GSM2258199: MN-296_TUMOR_ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: 10-15 frozen sections of each tumor block or dura sample were collected for ChIPseq experiments. Tissue was crosslinked with 1% formaldehyde, quenched with glycine, and washed with PBS. Nuclei were extracted by dounce homogenization and resuspended in nuclear lysis buffer containing 0.3% SDS. Chromatin was sheared by sonication with a Misonix S4000 in a 430A cup horn (60 minutes total, amplitude 30, 10 second pulses, 10 second rest). Soluble chromatin was incubated with magnetic beads coated with H3K27ac antibody (Abcam ab4729) overnight at 4°C. Chromatin was precipitated with magnet, washed extensively, and eluted with TE+1%SDS. Crosslinks were reversed and DNA was purified. Standard Illumina paired-end multiplexed library construction.  H3K27ac ChIP and input samples were sequenced for each tumor (1x75bp, HiSeq 2000).
attributes.tag: GEO Accession
attributes.value: GSM2258199