title: GSM3662037: HiC_Sac_sta_rep2; Sulfolobus acidocaldarius; Hi-C
library_construction_protocol: Cells were crosslinked, permeabilized, and treated with HindIII to digest cellular DNA. The DNA was end-labeled with biotin and subjected to priximity ligation. After reversal of crosslinks, the DNA was extracted with phenol:chloroform:isoamyl alcohol, precipitated in isopropanol, and sonicated. Libraries were constructed using NEBNext Ultra DNA Library Prep Kit for Illumina. Biotin-labeled DNA was purified with streptavidin before PCR. Sequencing was performed on the Illumina NextSeq 500 platform.
attributes.tag: GEO Accession
attributes.value: GSM3662037