title: Illumina HiSeq 4000 sequencing; Transcriptomic changes in C3H10T1/2 cells exposed to lipid deprivation
design: Transcriptomic changes in C3H10T1/2 cells exposed to lipid deprivation
library_construction_protocol: The C3H10T1/2 skeletal progenitor cell line was obtained from the RIKEN Cell Bank (Tsukuba, Japan) and was cultured in α-MEM with 1% P/S and 10% FBS. C3H10T1/2 cells seeded in 6-well plates, and medium was replaced with α-MEM with 1% P/S and 1% FBS or with α-MEM with 1% P/S and 10% FBS. Next, after 1, 3 or 6 hours, cells in plates were washed using ice-cold PBS. and total RNA was extracted using TRIzol.  Total RNA was extracted using TRIzol.  Library preparation were done using the KAPA stranded mRNA-seq kit (Roche).
attributes.tag: Experimental Factor: time
attributes.value: 3
attributes.tag: Experimental Factor: growth condition
attributes.value: fetal bovine serum; 1 percent