title: GSM4715161: A12; Homo sapiens; RNA-Seq
library_construction_protocol: High molecular weight RNA (>200 base pairs) was extracted from Xenograft samples using the miRNeasy Mini kit as per manufacturer's instructions (QIAGEN®). Residual genomic DNA was removed during the extraction using the RNase-free DNase set (QIAGEN®). RNA quality was analyzed using Eukaryote Total RNA Nano chips (Agilent Technologies), and all samples used for analysis had an RNA integrity number > 7. RNAseq libraries were generated from 0.1 μg total RNA using the TruSeq® Stranded mRNA kit (Illumina®) as per manufacturer's instructions. Sequencing libraries were quantified using the Kapa Library Quantification kit (Kapa Biosystems), and quality was assessed on the Agilent Bioanalyzer using DNA 1000 chips (Agilent Technologies). Libraries were sequenced on the HiSeq® 2000 sequencer (Illumina) for 2 × 50 cycles using the TruSeq® PE Cluster Kit v3-cBot-HS and TruSeq® SBS Kit v3-HS sequencing reagents (Illumina®) at Expression Analysis Inc [Q2Solutions]. Each lane was spiked with the PhiX Control v3 library (Illumina®) at a final concentration of 1 % (v/v) as a sequencing control.
attributes.tag: GEO Accession
attributes.value: GSM4715161