title: WGS of rattus norvegicus: adult male Han/Wistar liver
design: 100 ug of gDNA was sheared to 1-2kb fragments using the GeneMachine HydroShear standard shearing assembly and 1.5kb fragments isolated using 1% agarose gel size selection. Mate-pair libraries were circularized and constructed according to standard SOLiD Long Mate-Pair library protocols. Following library quantitation via TaqMan qPCR, libraries underwent emulsion PCR and bead enrichment according to standard SOLiD protocols. Enriched libraries were then sequenced 2x50bp using SOLiD 3 sequencing chemistry (Applied Biosystems, Burlington, ON).