title: GSM2257955: A549_shNT_Input; Homo sapiens; ChIP-Seq
library_construction_protocol: Ino80 ChIP were performed as described previously. Briefly, A549 cells were fixed using 1% formaldehyde for 10 min, and 0.125 M glycine was added to stop the fixation. Cells were harvested, and DNA was fragmented to 300–500 bp by sonication with a micro-tip attached to a Misonix 3000 sonicator. Immunoprecipitation was performed with antibodies conjugated to Dynabeads protein G beads (Life Technology). ChIP DNA was eluted, reverse cross-linked, extracted by phenol/chloroform, and precipitated. For ChIP-seq, 1 ng ChIP DNA or input DNA was used to generate sequencing libraries using the Nextera XT DNA sample preparation Kit (Illumina).  Nextera XT DNA sample preparation kit (illumina)
attributes.tag: GEO Accession
attributes.value: GSM2257955