title: GSM4714806: Hypothalamus DNA_INTSF37_EXP_Day 2; Tachycineta bicolor; MBD-Seq
library_construction_protocol: Brains were frozen on dry ice in the field and macrodissected into functional regions. Total RNA was extracted from each sample using Trizol (Invitrogen, Carlsbad, CA). Genomic DNA was isolated from each sample using a Quick-DNA Microprep Plus Kit (Zymo Research) and 300ng of DNA was sheared to an average size of 200bp using a Covaris E220 sonicator (Covaris). DNA Methyl capture was performed using a MethylCap kit (Diagenode). A negative control (the methyl binding domain protein was excluded during Methyl capture) and a fully methylated positive control (DNA treated with SssI CpG methyltransferase; New England Biolabs) were included. Total RNA was used for cDNA library construction using a TruSeq Stranded mRNA kit (Illumina) following the kit protocol. MethylCap-Seq libraries were generated using the NextFlex MethylSeq Kit (Bioo Scientific) following the kit protocol RNA-Seq and MethylCap-Seq
attributes.tag: GEO Accession
attributes.value: GSM4714806