title: GSM4462935: C5F1 ad rep1; Mus musculus; RNA-Seq
library_construction_protocol: Intestinal tissue as indicated was processed using the Qiagen Allprep RNA/DANN Kit according to manufacturer's instructions. For RNA sequencing, 4 µg of total RNA were depleted for ribosomal RNA using the RiboMinus Eukaryote Kit for RNA-seq (Invitrogen) following the manufacturer's instructions. The RiboMinus depleted RNA was then used for the generation of single-end RNA-seq libraries. cDNA libraries were size selected on a 2% agarose gel, fragments of 150-200 nt were excised (corresponding to insert sizes of 80-130 nt) and purified using the QiaQuick Gel extraction kit (Qiagen). Sequencing was performed on an Illumina Genome Analyser IIx.
attributes.tag: GEO Accession
attributes.value: GSM4462935