title: RNA Seq de novo assembly of Duddingtonia flagrans: a nematophagous fungus-Indiced 
design: Our study represents the de novo assembly and differential gene expression in Duddingtonia flagrans GenBank Assession no. JX979094 which is nematophagous fungus. Transcriptome sequencing of control and induced fungus was carried out. Fungus was induced by using nematode extract of root-knot nematodes. RNA was extracted using TRIzol reagent and RNeasy mini kit. mRNA was isolated from the total RNA using mRNA isolation kit from Roche. Barcoded cDNA library was prepared using the Ion Total RNA-Seq Kit v2RNA protocols. Sequencing was carried out using Ion Personal Genome Machine (PGM™) System (Version 3.0) along with Ion PGM™ Sequencing 300 Kit and Ion 316™ Chip.De novo assembly was carried out by CLC Genomic Workbench v 4.9. 