title: GSM2259069: T98G_EZH2_b2; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were incubated in hypotonic solution (10 mM KCL, 10 mM HEPES pH 7.5, 1.5 mM Mgcl2 and 2 mM DTT) for 5 mins and spun down by centrifugation. 1mg/ml digitonin (Cat #) was then added to the lysate resuspension and further incubated for 10 min with constant mixing. Cells were then mechanically lysed using dounce homogenizer (15 times) and spun down. Pelleted cells were then resuspended in NP40 lysis buffer (150 mM KCL, 50 mM hepes pH 7.5, 5 mM EDTA, 0.5 % IGEPAL and 2 mM DTT) and subjected to mild sonication (6 cycles, 10 sec on and 30 sec off). All the steps in this procedure were performed at 4oC.
attributes.tag: GEO Accession
attributes.value: GSM2259069