title: GSM1481098: mRNAs from Burkholderia gladioli BSR3 wild type after 24h LB liquid culture; Burkholderia gladioli; RNA-Seq
library_construction_protocol: All bacteria strains were grown in 2 ml of LB broth at 28°C with continuous shaking until reach mid-expotential phase (OD600 = 0.5), and subcultured for 10h or 24h in identical condition. Total RNA was extracted from 4 ml of each bacterial cultures using RNeasy Midi Kit (Qiagen, Valencia, CA) and residual genomic DNA contamination was subsequently removed using an RNase-Free DNase set (Qiagen, Valencia, CA) according to the manufacturer’s protocols. After DNase I treatment, a MICROBExpressTM bacterial mRNA enrichment kit (Ambion, Carlsbad, CA) was used to remove bacterial rRNA from the total RNA sample. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM1481098