title: GSM4471892: KO PAB rep 1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted from 10 mg of RV using Qiagen RNeasy Fibrous Tissue Mini Kit (cat. # 74704). DNaseI treatment was performed in the column according to the manufacturer's protocol. 60 uL of total RNA were recovered with concentrations ranging from 72.7 to 180.6 ng/μl and with A260/280 nm ranging from 1.89 to 2.11. RNA quality and integrity were evaluated by capillary electrophoresis on the Agilent 2100 Bioanalyzer platform (Agilent Technologies). The sequencing library from mRNA was prepared using TruSeq Stranded mRNA Library Preparation Kit (Illumina; San Diego, CA)
attributes.tag: GEO Accession
attributes.value: GSM4471892