title: 16S rRNA sequence: Antarctic Marine Sediment
design: PCR reaction was carried out with a thermal cycler (Thermo Scientific, USA), using 25 ?L of KAPPA HiFi HotStart Ready Mix (KAPPA Biosystems) polymerase, 5 ng of DNA and 0.2 ?M of each primer, under the following conditions: 95°C for 3 min, 30 cycles of 95°C for 30 s, 55°C or 67°C for 30 s (for Bacteria and Archaea, respectively), 72° C for 30 s and a final extension of 72°C for 5 min.