title: Buchnera aphidicola soybean aphid genome
design: The cDNA library (3.6 x 10-5 nmol) consisting of four samples of A. glycines biotype 1 and 2 was sequenced on one flow cell lane using the Illumina MiSeq platform at the Ohio State University Comprehensive Cancer Center. Four fluorescently labeled nucleotides and a specialized polymerase were used to determine the clusters base by base in parallel. The mean library insert sequence length was 274 bp and both ends of the library were sequenced to generate 100 nt raw paired-end reads. Illumina Analysis Package CASAVA 1.8.2 was used to perform bcl conversion and demultiplexing. Image deconvolution and quality value calculations were carried out using the Illumina GA pipeline v1.6.