title: GSM2258276: RF8-4; Danio rerio; OTHER
library_construction_protocol: Extraction with SEL buffer + Proteinase K (50 mM KCl, 2.5 mM MgCl2, 10 mM Tris pH 8.3, 0.045% Igepal, 0.045% Tween-20, 0.05% Gelatine and 0.1 mg/ml Proteinase K). Samples were incubated for 1 h at 60 °C and 15 min at 95 °C GFP sequences were amplified by PCR with primers complementary to GFP, including an 8 bp barcode sequence and adapter sequences for Illumina sequencing. Samples were then pooled and subjected to magnetic bead cleanup (AMPure XP beads – Beckman Coulter). Finally, sequenceable libraries were generated by a second round of PCR with indexed primers from Illumina’s TruSeq Small RNA Sample Prep Kit.
attributes.tag: GEO Accession
attributes.value: GSM2258276