title: Late bulk
design: DNA was isolated from 100mg fresh leaves from selected F3 plants by using the DNeasy Plant Mini Kit (QIAGEN). The DNAs isolated from 37 earliest flowering F3 individuals were mixed in an equal ratio to construct the late bulk. TruSeq DNA Library Prep Kit was used for library preparation. Whole-genome pair-end sequencing was conducted by Illumina HiSeq3000 with read length of 150bp and > 30x genomic coverage.