title: GSM2259181: IRF4_10ng; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% formaldehyde for 8 min at room temperature, quenched with 1.25M Glycine, twice washed with PBS.  Chromatin was sonicated for shearing DNA size from 140 to 300 bp. Chromatin fragments were immunoprecipitated overnight at 4 °C with Dynabeads Protein A or G bound to anti-BATF or anti-IRF-4 (sc-6059x, Santa Cruz Biotechnology).  For ChIP-seq, ChIPed DNA was eluted and reverse-crosslinked for 6 hr. DNA was purified with phenol:chloroform extraction followed by ethanol precipitation. For ChIP-exo, see library construction protocol. For ChIP-seq, libraries were prepared using ThruPLEX-FD kit (Rubicon Genomics).  For ChIP-exo,  ChIPed DNA were performed by the following enzymatic reactions; End polishing, ligation of P7 exo-adaptor, nick repair, exonuclease reaction. DNA was eluted and reverse crosslinked. The libraries were generated by P7 primer extension, ligation of P5 exo-adaptor, PCR amplification for 12 cycles and were cleaned up by AMPure XP.  All libraries were sequenced using Illumina HiSeq 2500 as single reads extending 50 bases.
attributes.tag: GEO Accession
attributes.value: GSM2259181