title: GSM4151307: 65079 L KO -; Mus musculus; RNA-Seq
library_construction_protocol: 40 week old male WT and SUCNR1 KO mice were randomly distributed over 2 treatments groups(-/+ hydroquinone). Every group contained 4 animals. Anterior segments (cornea, iris and lens) were removed to expose the posterior eyecup. The retina was carefully peeled off, and the eyecup containing the RPE, sclera and choroid were stored in RNAlater solution.   For RNA isolation, the RPE was removed gently from the choroidal layer using fine forceps. The brown clumps of RPE cells were dissolved in RLT Buffer from RNeasy Micro Kit after which RNA was extracted using the RNeasy Micro Kit. Illumina Novaseq 6000 sequencer was used for 1x 150 bp paired-end sequencing, sequencing an average of 20 million reads per sample. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4151307