title: GSM3660283: M2 input; Homo sapiens; MeDIP-Seq
library_construction_protocol: The genomic DNA in each group was extracted using a phenol and chloroform method and sonicated with a Bioruptor sonicator (Diagenode) to generate 200–700 bp fragments. The libraries were constructed following the Illumina genomic DNA protocol and adaptor-ligated DNA was immunoprecipitated using 5′-methylcytidine antibodies. The MeDIP products and supernatant DNA were then purified using MinElute columns (Qiagen, Valencia, CA, USA) and eluted in 16 μL of elution buffer (EB; Qiagen). The products were verified using qPCR, with 5 μL of DNA examined per sample using single-end Illumina PCR primers. The methylated DNAs were amplified in situ using a TruSeq Rapid SR Cluster Kit (#GD-402-4001, Illumina), and the quality of the fragments between 300–800 bp was evaluated using an Agilent BioAnalyzer. Sequencing was performed using an Illumina HiSeq 4000 according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3660283