title: GSM4463281: HCC1806.SCAPko.g1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using Rneasy Plus Mini Kit (QIAGEN). RNA was quantified using RNA 6000 Pico Kit on a 2100 Bioanalyzer (Agilent). RNA-Seq libraries were prepared using KAPA mRNA Hyper Prep Kit from 100 ng RNA input according to manufacturer's instructions (Roche). The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and qPCR using the KAPA Library Quantification Kit (Roche). Uniquely indexed libraries were pooled in equimolar ratios and sequenced on Illumina NovaSeq6000 runs with paired-end 50bp reads.
attributes.tag: GEO Accession
attributes.value: GSM4463281