title: GSM4151455: HEK293T AQ-seq siTUT4/7; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol (Invitrogen). Total RNA was mixed with thirty synthetic miRNA-like spike-in oligos. The RNA mixture was size-fractionated by 15% urea-polyacrylamide gel electrophoresis to enrich miRNA species. Small RNA libraries were constructed using either TruSeq small RNA library preparation kit (Illumina) according to manufacturer's instructions or AQ-seq as follows: miRNA-enriched RNA was ligated to 3? randomized adapter with 20% PEG 8000, then ligated to 5? randomized adapter with 20% PEG 8000, reverse-transcribed, and amplified. The cDNA was sequenced using a HiSeq 2500 platform.
attributes.tag: GEO Accession
attributes.value: GSM4151455