title: GSM4763927: PDX Abemaciclib 1; Homo sapiens; OTHER
library_construction_protocol: For H3K27ac FiTAc-seq, FFPE sections containing >80% tumor cell enrichment were used and processed as previously described (Cejas P et al, Nat. Methods, 2019; Font-Tello A et al, Nat. Protocols, 2020). Briefly, chromatin was prepared from 5-10 sections (10 ?m each) per case. Sections were washed with xylenes to remove paraffin, rehydrated in an ethanol/water series and incubated overnight with lysis buffer (1%SDS, 50 mM Tris-HCl pH8, 10 mM EDTA) on a thermomixer at 65C, 1000rpm). Samples were put on ice,  suplemented with protease and deacetylase inhibitors and sonicated with Covaris E220 instrument for 10 min (PIP105, 5%DF, 200cbp) in 1mL AFA Fiber milliTUBEs. 2.5?g -5?g of chromatin were pre-cleared, immunoprecipitated overnight with 3 ?g of H3K27ac antibody (Diagenode, cat#A1723-0041D, lot#C15410196) on protein A and G magnetic beads and finally washed, eluted and DNA purified. FiTAc-seq libraries were constructed using Accel-NGS 2S DNA library kit from Swift Biosciences.
attributes.tag: GEO Accession
attributes.value: GSM4763927