title: WGS of metagenomic and metatranscriptomic sequencing of the human gut microbiome:    Subject 13948544
design: Participants deposited each bowel movement into a plastic commode     collection bowl, and then using a specially designed spoon attached to a collection     tube cap, feces were scooped along with into a tube containing RNAlater     (PMID24843156). At each time point, participants stored the specimen in the RNAlater     fixative at ambient temperature until the specimen collected from the second bowel     movement was produced. Each set of stool specimens were then placed in a special     mailing kit and returned at ambient temperatures by overnight mail. Upon receipt,     the collection tubes were immediately placed into -80 degrees C freezers. Nucleic     acids were extracted as detailed in this publication     PMID24843156.