title: GSM4212451: Sorted microglia from pooled P0 brain of WT mice, treated with LPS+interferon b, replica 1; Mus musculus; RNA-Seq
library_construction_protocol: 2. Macrophages, microglia from healthy and EAE mice: Total immune cells were isolated from spinal cords following tissue digestion and following a classic Percoll gradient protocol. Next, they were incubated with fluorochrome coupled antibodies for leukocytes (anti-CD45 FITC), T-cells (anti-CD3 APC), myeloid cells (anti-Cd11b PE and F4/80 PE-Cy7) and DAPI for dead cells. Live CD45+Cd11b+ cells were sorted in RLT lysis buffer and RNA were extracted using RNAeasy Plus Micro Kit (QIAGEN), Quality control (QC) of extracted RNA were performed and high quality ones were sequenced. Total RNA from sorted myeloid CD45+Cd11b+ cells from WT and KO spinal cords of EAE mice was polyA enriched using  Illumina TrueSeq2.0 kit
attributes.tag: GEO Accession
attributes.value: GSM4212451