title: 400bp PCR-Free PE Shotgun Library
design: DNA fragment sizes were prepared using the TruSeq DNA Sample Preparation Kit version 2 according to the manufacturer’s protocol, using 350bp to 450bp DNA fragments with no PCR amplification (PCR-free).  Sequenced on an Illumina MiSeq as PE with 250bp per end with Illumina MiSeq version 2 chemistry