title: Illumina HiSeq 2500 paired end sequencing; The tomato MYB21 acts in ovules to mediate jasmonate-regulated fertility (RNASeq)
design: The tomato MYB21 acts in ovules to mediate jasmonate-regulated fertility (RNASeq)
library_construction_protocol: Flowers of wild type, jai1-1 and myb21-2 were harvested in a very strict time window of 30 min starting at 7 h after the onset of light period, carpels dissected, transferred to liquid nitrogen and stored at -80 C.  Plants were grown in a controlled growth chamber with 16 h light (110 µmol photons*m-2*s-1) at 26°C and 8 h dark at 20°C, both at 70 % humidity.  RNA isolation from homogenized material (ball mill) was performed using the RNeasy Plant Mini Kit (Qiagen) according to the supplier's instructions followed by routine DNase treatment using DNA-freeTM Kit (Thermo Scientific). RNA quality was tested by capillary electrophoresis using QIAxcel Advanced System (Qiagen).  A one μg subsample of total RNA from each of 9 RNA extracts (1 stage×3 genotypes×3 biological replicates) were sent to the GATC Biotech AG (Konstanz, Germany, https://www.eurofinsgenomics.eu/) and used to produce Illumina HiSeq 2500 strand-specific 2x150 bp paired-end reads.
attributes.tag: Experimental Factor: genotype
attributes.value: wild type genotype