title: GSM4472085: 10_Ps2Ex3M1_WT_6month_F3_86_Fem; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was extracted from pooled larvae using the mirVanaTM miRNA isolation Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). rRNA was depleted from total RNA samples using the rRNAseH rRNA depletion method [Adiconis et al, Nat Methods 2013 Jul (10) 7 623-629] and a combination of human/rat/mouse sequences. 500ng of RNA was used for rNRA depletion, followed by the KAPA Hyper Library Prep kit, fragmentation at 85 degrees (C) for 6 mins and 11 PCR cycles. Sequencing was performed by the Australian Cancer Research Foundation Cancer Genomics Facility in Adelaide, South Australia
attributes.tag: GEO Accession
attributes.value: GSM4472085