title: Gene expression Danio rerio adult retina, 4 days post optic  nerve crush,, replicate 2
design: RNA was extracted and purified from retinas dissected from adult  fish, 4 days post optic nerve crush. RNA was extracted from pooled retinas  using the RNeasy Micro kit (Qiagen) and concentrated using the RNA Clean  & Concentrator kit (Zymo).cDNA libraries were generated for each RNA  sample using Tru-Seq Stranded Total & mRNA Sample Prep Kits, (Illumina  20020595). Each cDNA library was indexed for multiplexing and subsequently  sequenced on four lanes of the Illumina Hiseq2000. Libraries were sequenced  at 50 bp, 3040 million paired-end reads/sample