title: GSM2869491: Mouse BM_4; Mus musculus; RNA-Seq
library_construction_protocol: Human CD34+ mobilized peripheral blood cells were isolated by human CD34 MicroBead Kit (MiltenyiBiotec), according to the manufacturer's protocol after collecting red cells by Ficoll-Paque PLUS (GE). MPP cells (CD34+CD38-Thy1-CD45RA-CD49f-) were isolated from Human CD34+ mobilized peripheral blood cells by flow cytometry sorting using CD34, CD38, Thy, CD45RA and CD49f antibodies. RNA libraries were prepared for sequencing using TruePrep DNA Library Prep Kit V2 for Illumina ( Vazyme, cat # TD513).
attributes.tag: GEO Accession
attributes.value: GSM2869491