title: GSM4712641: Neo4p13_CA; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from native enriched chromatin digested with micrococcal nuclease and protein-DNA complexes were isolated with antibody. For library preparation we used NEBNext Ultra II DNA library prep kit for Illumina (E7645) and NEBnext Multiplex oligos for Illumina (NEB E7335) using the manufacturer's recommendations. Libraries were size-selected using AMPure XP magnetic beads (Beckman Coulter) to exclude polynucleosomes (200bp + adaptors size) and PCR amplified before submission to 75-bp single-end sequencing on Illumina NextSeq 500 platform.
attributes.tag: GEO Accession
attributes.value: GSM4712641