title: Illumina of bacteria:flour
design: A 464-nucleotide sequence of the V3-V4 region of the 16S rRNA gene (Escherichia coli positions 341 to 805) was amplified starting from the previously extracted genomic DNA. Unique barcodes were attached before both the forward and primers. The amplicons were cleaned using the Agencourt AMPure kit (Beckman coulter). DNA concentrations of the amplicons were determined using the Quant-iT PicoGreen dsDNA kit (Invitrogen). The quality of the generated amplicon libraries was evaluated by a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA) using the High Sensitivity DNA Kit (Agilent).