title: GSM4150379: sciPlex4_A549_MCF7_HDACi; Homo sapiens; RNA-Seq
library_construction_protocol: 24 hours after drug exposure, cells were harvested following the sci-Plex protocol as described in the methods section of our manuscript. Nuclei were isolated in-well and nuclei barcoded by fixation of well-specific barcode containing oligonucleotides. After fixation wells were pooled and nuclei pellets flash frozen until sci-RNA-seq library preparation. Nucle were subjected to two or three level sci-RNA-seq following the protocols described in Cao et al. 2017 Science 357(6352) p. 661-667 (two level sci-RNA-seq) and Cao et al. 2019 Nature 566(7745) p. 496-502 (three level sci-RNA-seq).
attributes.tag: GEO Accession
attributes.value: GSM4150379