title: 16S rRNA gene profiling results from a synthetic microbial community using a traditional PCR/NGS approach: 100 input molecules: Sample Synthetic microbial community
design: "The protocol utilized a two-step PCR protocol. In the first step, PCR was performed using modified 341F and 806R primers that amplified the V3-V4 regions of 16S rRNA genes and which incorporated universal sequence tails at their 5' ends. In the second step, a PCR was again used, but to amplify PCR amplicons obtained from the first step PCR. The second step PCR utilized primers containing complementary sequences to the universal tails and included additional 454 sequencing-specific nucleotides, and specimen-specific MIDs."