title: Metagenome of biocathode: DNA biofilm electrode 4
design: Amplification of the hypervariable V4 region of the 16S rRNA gene. Dual-indexed Illumina compatible libraries were produced in a single PCR step. Fusion primers with target-specific positions and Fluidigm CS oligos (51 ends) were used for the primary PCR. Secondary, PCR targeting the CS oligos was use to add sequences necessary for Illumina sequencing and unique indexes. Invitrofen SequalPrep DNA normalization plates were used with PCR products. The pooled samples were sequenced using an Illumina MiSeq flow cell.