title: GSM4715022: F105H NET-seq Rep 1; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Cells were lysed via 10 1 minute grinds at maximum amplitude in a mikro dismembrator II. The grinding cup was chilled in liquid nitrogen for 1 minute between each grind. Pol I elongation complexes were isolated from the cell lysate via immunoprecipitation with anti-HA magnetic beads (tagged gene = RPA135 C-terminal (3x-HA 7x- his) . Associated RNA transcripts were then isolated using an acidic phenol-chloroform extraction. The RNA transcripts were then 3' appended with a DNA linker and reverse transcribed to produce complementary DNAs (cDNAs). The cDNAs were circularized and amplified by PCR to produce high-throughput sequencing libraries.
attributes.tag: GEO Accession
attributes.value: GSM4715022