title: GSM4700900: EC-enriched murine lung cells; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized by overdose of anaesthetics (Ketaminol/Rompun) and briefly perfused with PBS. Lungs were harvested and left lobes of the lungs were dissociated with 0.5 mg/mL Collagenase H (Roche), 0.5 U/mL of Dispase II (Sigma), 1 U/mL DNase I (Thermo Scientific) and 1% (w/v) BSA (Biowest). Dissociated cells were washed once with PBS and stained with Alexa-antibody-conjugates, specific for CD31. CD31+DAPI- were sorted using BD Influx flow. The sorted fraction had approximately 60-70% endothelial cells (CD31+), and that was used for scRNAseq. Subsequent cDNA purification, amplification (12 cycles) and library construction (sample index PCR 14 cycles) was performed as instructed Sample libraries were sequenced on the Illumina NovaSeq 6000 system using S1 flow cell (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM4700900