title: 16s-seq of soil microbiome:sorghum rhizosphere
design: The 16S r-RNA primers were designed with Illumina adapters and dual indices (8 base pair Golay codes on 3 and 5 ends) as described (Caporaso et al. 2012). The forward primer 515F and reverse primer 806R were used to amplify the V4 region of the 16S r-RNA. The fungal marker ITS2, primers ITS3 and ITS4 followed similar design of V4 primers in having adapters and dual indices and were used to amplify ITS2 gene