title: GSM1836743: TP23_1; Homo sapiens; OTHER
library_construction_protocol: DNA was isolated 2 days after initial infection (TP0) and at end of experiment (TP23) using Qiagen DNeasy Blood and Tissue Kit A two-step PCR method was employed to amplify inserted CRISPR-Cas9 RNA guide sequences and append molecular barcodes and Illumina adapter sequences. We modified our method to be compatible with Illumina TruSeq sequencing and obtain sufficient sequence information from a single-end 50-cycle sequencing run.
attributes.tag: GEO Accession
attributes.value: GSM1836743