title: GSM4763685: Nanos2RFP-Positive germ cells; Mus musculus; RNA-Seq
library_construction_protocol: Germ cells were sorted, single cell suspension loaded on a Chromium controller (10x Genomics) to generate single cell GEMS (Gel Beads-In-Emulsions) where full length cDNA was synthesized and barcoded. Subsequently the GEMS are broken and cDNA from each single cell are pooled. Following cleanup using Dynabeads MyOne Silane Beads (Thermo Fisher, 370020), cDNA is amplified by PCR.  The amplified product is fragmented to optimal size before end-repair, A-tailing, and adaptor ligation. Final library was generated by amplification
attributes.tag: GEO Accession
attributes.value: GSM4763685