title: GSM4714944: BG-input-2; Mus musculus; ChIP-Seq
library_construction_protocol: Approximately 5 x 10^6 Bergmann glial cells isolated as above was fixed in 1% formaldehyde for 10 min at room temperature (RT), followed by quenching in 125 nM glycine for 5 min. Cell nuclei were collected and lysed in lysing buffer (50 mM HEPES/KOH pH 7.6, 1 mM EDTA, 140 nM NaCl, 10 % v/v Glycerol, 0.5 % NP-40, 0.25 % v/v Triton X-100) supplemented with protease inhibitor. Nuclei were subjected to sonication to acquire DNA fragments of 200-350 bp. The sonicated chromatin was applied to immunoprecipitation by incubation with 1.5μg H3K9me3 antibody (rabbit, Abcam, #ab232324) overnight at 4 °C, followed with collection using 30 μL protein A/G plus agarose beads (Millipore). The ChIP libraries were prepared using KAPA HyperPrep Kits (Roche, 7962347001)
attributes.tag: GEO Accession
attributes.value: GSM4714944