title: GSM4928984: Aorta_Tie2Ctrl; Mus musculus; RNA-Seq
library_construction_protocol: The aortas were harvested in PBS and their surrounding adipose tissues were removed. The aortas were then minced into pieces and digested in DPBS containing 1 mg/ml collagenases Type I (Gibco #17100-017), II (Gibco #17101-015) and IV (Gibco #17104-019) each for 30 minutes at 37oC water bath with mild mixing for a few times during the digestion. After the digestion, the cell suspension was filtered through a 70 μm cell strainer. The red blood cells were lysed with ACK Lysing buffer (Gibco # A10492-01) according to the instructions. For sample 1-6, we use the Chromium Single-Cell 3′RNA-sequencing system (10x Genomics, Pleasanton, CA) with the Reagent Kit v3.1 according to the manufacturer's instructions. For sample 7-10, we use the Chromium Single-Cell 3′RNA-sequencing system (10x Genomics, Pleasanton, CA) with the Reagent Kit v2.1 Sample libraries were sequenced on the Illumina NovaSeq 6000 system using S2 flow cell (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM4928984