title: Gen58Bacteria
design: Genomic DNA were extracted from soil at the root zone.  Forward primer  name 341F Forward primer sequence (5' to 3') CCTACGGGNGGCWGCAG   Reverse primer name 785R Reverse primer sequence (5' to 3') GACTACHVGGGTATCTAAKCC  were used. Illumina adapter overhang nucleotide sequences were added to the primer. The PCR reaction was performed.