title: GSM4763755: GATA6-AS1 shRNA Day2_Replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cardiac differentiation was induced on IMR90 hiPSCs transfected with GATA6-AS1 shRNA or control shRNA using a growth factor-guided protocol. RNA samples from cells at differentiation day 2 and day 5 were prepared using Bio-Rad RNA extraction kit and 1 µg of total RNA/sample was used for the construction of sequencing libraries using Illumina TruSeq RNA Sample Prep Kit. Libraries were generated using the Illumina standard TruSeq protocol and next-generation sequencing was done through an Illumina HiSeq 2500, 75bp paired end sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4763755