title: Illumina sequencing of simian arteriviruses from experimentally infected crab-eating macaques
design: vRNA was isolated from cell culture supernatant or plasma stored in TriZol, extracted and reverse transcribed. PCR amplified full genome with 10, overlapping amplicons. Prepped with the Nextera XT library prep kit and sequenced on a 2 x 300 cycle kit with 1% PhiX.