title: GSM4150502: Ras/Src HSD rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA from pooled dissected tissue was extracted with the QIAGEN RNeasy Mini Kit. RNA-seq libraries were prepared from a minimum of 10ng of total RNA using the Illumina Truseq mRNA stranded library prep kit (Illumina, San Diego, USA) according to the manufacturer's protocol.  Library quality was checked on a Bioanalyser HS DNA chip and concentrations were estimated by Qubit measurement. Libraries were pooled in equimolar quantities and sequenced on a Hiseq2500 using paired end 100bp reads. At least 35 million reads passing filter were achieved per sample.
attributes.tag: GEO Accession
attributes.value: GSM4150502