title: Drosophila subpulchrella head body RNAseq replicate 2
design: All individuals were mated and precisely 3-days-old. Female flies were very briefly anesthetized with CO2 and heads were collected with a clean razor blade. Dissections were performed within a 1-hour window always at the same circadian zeitgeber time (ZT1-ZT2; with light turning on at 8 am, and the dissections being performed between 9 am and 10 pm). We collected 3 biological replicates for each species, each sample contained 15 heads. Dissected heads were immediately transferred into a low retention Eppendorf tube containing 100 mL TRIzol (Invitrogen) and RNA was extracted immediately post dissection.