title: GSM4150416: d41 S16 CD8 TCM; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted following the double extraction protocol:  RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (TRIzol, Thermo Fisher Scientific, Waltham, MA, USA) followed by a Qiagen RNeasy Micro clean-up procedure (Qiagen, Hilden, Germany). All mouse RNAs were analyzed on Perkin Elmer Labchip GX system (Perkin Elmer, Waltham, MA, USA) for quality assessment with a median RNA Quality Score of 9. cDNA libraries were prepared using 500pg of total RNA and 1ul of a 1:200,000 dilution of ERCC RNA Spike in Controls (Ambion) using SMARTSeq v2 protocol [1] except for the following modifications: 1. Use of 20µM TSO, 2. Use of 250pg of cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system. All samples were subjected to an indexed PE sequencing run of 2x51 cycles on an Illumina HiSeq 2000 (16 samples/lane).
attributes.tag: GEO Accession
attributes.value: GSM4150416