title: GSM3271505: Huh7_siControl_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from 3 different cell lines in 3 replicates each using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instruction. RNA quality control was performed with Bioanalyzer 2100 (Agilent) using the Eukaryote Total RNA Nano analysis kit from Labgene and RNA concentration was measured by Quanti-iTTM RiboGreen RNA assay kit (Thermo Fisher Scientific). 200ng of RNA was utilized for library preparation with the TruSeq Stranded total RNA kit plus Ribozero Gold (Illumina). The library quality check was performed using the DNF-473-33 - SS NGS Fragment analysis kit (Advanced Analytical Technologies Inc).
attributes.tag: GEO Accession
attributes.value: GSM3271505