title: RNA-Seq of Homo sapiens
design: Total RNA was extracted from PBMCs using RNeasy Mini (Qiagen, Hilden, Germany) according to the manufacturer s protocol and the RNA quality was determined by Agilent RNA 6000 pico kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). The mean and SD RIN value was 8.85 (0.94). The library preparation, RNA-Seq, data processing and analysis were performed at the National Genomics Infrastructure, Science for Life Laboratory, Stockholm, Sweden. The libraries were prepared from 100 ng of total RNA samples using Illumina TruSeq  Stranded mRNA Library Prep Kit with poly-A selection following manufacturer s guidelines. The clonal cluster amplification was carried out with Illumina cBOT System. The pooled libraries were sequenced on HiSeq2500 (HiSeq Control Software 2.2.58/RTA 1.18.64) with a 2x126 setup using 'HiSeq SBS Kit v4' chemistry.