title: GSM2259084: EZH2_iCLIP; Homo sapiens; OTHER
library_construction_protocol: Cells were crosslinked with 0.15J/cm2 of UV254 using stratalinker 2400. Crosslinked cells were lysed using NP40 lysis buffer (50mM Tris, pH7.4; 100 mM Nacl; 1% Igepal CA-630; 0.1%SDS;0.5% sodium deoxycholate and protease inhibitor). Lysates were then treated with 200U/ml Turbo DNaseI and 20U/ml of RNaseI followed by incubation at 37C for 2 mins.
attributes.tag: GEO Accession
attributes.value: GSM2259084