title: GSM4462586: MMBN37_FRC steady state; Mus musculus; RNA-Seq
library_construction_protocol: Lymph Nodes (sample 1, n=6 untreated mice) and/or Spleens (sample 2, n=6 untreated mice ; sample 3 n=6 diphteria-toxin-treated mice) from 12 Wt1CreERT2 RosatdTomato CD64DTR  mice were digested for 40 min at room temperature with dispase/collagenase/DNase . All subsequent procedures were at 0–4°C. CD45- cells were enriched using MACS anti-CD45 microbeads and passing them through a MACS LS column. CD45- cells were incubated on ice for 30 min with 2.4G2 antibody to block Fc receptors and stained for the  surface markers CD45, gp38, CD31, and VCAM1. tdTomato- FRC and tdTomato+ RPF were sorted using a  FACSAria III and processed according to the RNeasy Plus Microkit instruction from QIAGEN. RNA library was prepared by the IGBMC GenomEast plateform ('France Génomique' consortium)
attributes.tag: GEO Accession
attributes.value: GSM4462586