title: GSM3270936: CBX4+/+ hMSC differentiated from hESC, early passage for RNA-seq [RNA-MSC-WT1]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with the RNeasy Mini Kit (Qiagen). To obtain the genomic DNA fragments of interest for ChIP-seq, the cells were crosslinked and lysed, and then the chromatin was sheared into fragments using sonication. Then samples were incubated with anti-H3K4me3 antibody (Abcam, ab8050, lot#GR3190162-1) or anti-FLAG antibody overnight. The DNA was de-crosslinked and extracted on the next day. 1.5 μg of total RNA was used to construct RNA sequencing libraries by NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina following the manufacturer's recommended protocol. The ChIP-sequencing library and copy number variation analysis sequencing library were constructed by NEB Next Ultra DNA Library Prep Kit (NEB) for Illumina according to the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3270936