title: GSM4712883: PPF WT 1; Mus musculus; RNA-Seq
library_construction_protocol: Pleuroperitoneal folds (PPFs) were isolated from wild-type E12.5 mouse embryos by cutting embryos just above the hindlimbs and below the forelimbs and removing the heart and lungs cranially, leaving the trunk with attached nascent diaphragm. The PPFs were manually dissected and stored in RNAlater (Thermo Fisher, #AM7020) at -80°c. RNA was isolated using a RNeasy Micro Kit (Qiagen, #74004), RNA quality confirmed with RNA TapeStation ScreenTape Assay (Agilent, # 5067-5576), and sequenced using TruSeq Stranded mRNA Library Preparation Kit with polyA selection (Illumina) RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4712883