title: mock-extract of Rnase free water on regular miRNeasy column batch 2
design: sRNA libraries were made using the TruSeq small RNA library preparation kit (Illumina) according to the manufacturer's instructions, except that the 3' and 5' adapters were diluted 1:3 before use.  PCR-amplified libraries were size selected using a PippinHT instrument (Sage Science), collecting the range of 121-163 bp.  Completed, size-selected libraries were run on a High Sensitivity DNA chip on a 2100 Bioanalyzer (Agilent) to assess library quality.  Concentration was determined by qPCR using the NEBNext Library Quant kit (NEB).  Libraries were pooled, diluted and sequenced with 75 cycle single-end reads on a NextSeq 500 (Illumina) according the manufacturer's instructions.