title: Molecular Biogeography of Planktonic and Benthic Diatoms in the Yangtze River
design: Barcoded primers DIV4for (5_GCGGTAATTCCAGCTCCAATAG_3) and DIV4rev3 primers (5_CTCTGACAATGGAATACGAATA_3) targeting bacterial the V4 region of the diatom 18S ribosomal RNA gene was amplified by PCR