title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of fetal urinary bladder and lung tissue
design: RNA-seq of fetal urinary bladder and lung tissue
library_construction_protocol: Collection of clinical samples by surgery. Fresh tissues were dissected and immediately submerged in 5 volumes of RNAlater Stabilization Solution (Cat No. AM7020, Thermofisher Scientific, Waltham, MA), incubated at 4°C overnight and then transferred to -20°C. RNA was extracted from one fetal lung and seven urinary bladder samples by using TissueLyser LT (Cat No. 85600, QIAGEN, Hilden, Germany) and RNeasy Fibrous Tissue Mini Kit (cat. no. 74704, QIAGEN, Hilden, Germany). The RNA was measured both quantitatively and qualitatively using NanoDrop (Thermofisher Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent, Santa Clara, CA) instruments.  Illumina TruSeq mRNA library protocol according to standard procedures.
attributes.tag: Experimental Factor: urinary bladder
attributes.value: organism part