title: GSM4101151: ChIP-seq_CTCF_diff; Rattus norvegicus; ChIP-Seq
library_construction_protocol: DNA for ChIP-seq:Purified rat SCs (∼20 million cells) were fixed for 10 min at room temperature with 1% formaldehyde-containing medium. Nuclei were isolated and sonicated in sonication buffer (10 mM Tris-HCl [pH 8.0], 1 mM EDTA, 0.5 mM EGTA, and protease inhibitor cocktail). Sonicated chromatin (∼300 μg) was used for immunoprecipitation by incubation with anti-CTCF, anti-H3K27ac antibody overnight at 4°C. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB catalogue number E6240L) and then run on the Illumina sequencer HiSeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM4101151