title: RNA-seq of human: adult female colon non-IBD: Sample UNC_NIBD_011_1
design: "RNA was isolated from fresh-frozen human colon tissues using the Qiagen RNeasy Mini Kit (Valencia, CA), which preserves both miRNA and mRNA content. This kit uses a column-based DNase treatment to eliminate DNA contamination. RNA purity and integrity were assessed with Thermo Scientific NanoDrop 2000 (Waltham, MA) and Agilent 2100 Bioanalyzer (Santa Clara, CA), respectively. Libraries were prepared using the Illumina TruSeq polyA+ Sample Prep Kit."
attributes.tag: alignment_software
attributes.value: GSNAP