title: RNA-seq of Ambrosia artemisiifolia: female flower
design: For poly-A based mRNA enrichment and cDNA synthesis the Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) was used according to manufacturer's instructions. The RNA-Seq was performed using Illumina HiSeq2000 system. The hybridization onto a flow cell, the dsDNA fragments were blunt-ended through an end-repair reaction and both ends were ligated to platform-specific double-stranded bar-coded adapters. The samples were run in one lane using multiple indexing adapters.