title: GSM2259961: Adelman_Dmel_S2_4sU-RNA-seq_control_rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was extracted from nuclei using Trizol reagent (Invitrogen). RNA quality was assessed using a Bioanalyzer NanoChIP (Agilent). Ribosomal RNA was removed prior to library construction by hybridizing to ribo-depletion beads that contain biotinylated capture probes (Ribo-Zero, Epicentre). RNA was then fragmented and libraries were prepared according to the TruSeq Stranded Total RNA Gold Kit (Illumina) using random hexamer priming. Illumina Spike-ins were used for normalization.
attributes.tag: GEO Accession
attributes.value: GSM2259961