title: GSM1196064: FC_miRNA; Capra hircus; RNA-Seq
library_construction_protocol: On day 90(d90), caprine embryos (gestation period 150 days) were collected into sterile physiological saline immediately after removal from the reproductive tract of slaughtered goats at a local abattoir. These tissues were snap-frozen in liquid nitrogen and stored at −80°C until use. And RNA was extracted using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols.Low molecular weight RNAs were separated by 15% polyacrylamide gel electrophoresis (PAGE), and RNA molecules in the range of 18–30nt were enriched and ligated with proprietary adapters to the 5′ and 3′ termini. A reverse transcription reaction followed by low-cycle PCR was performed to obtain sufficient product for Solexa technology
attributes.tag: GEO Accession
attributes.value: GSM1196064