title: scRNAseq of adult male mouse on Western diet
design: Tumor infiltrating lymphocytes were sorted to at least 99% purity based on CD45 staining and single cell suspensions were loaded on a Chromium Single Cell Controller instrument (10x Genomics, Pleasanton, CA, USA) to generate single-cell gel beads in emulsion (GEMs). After sorting and processing, 8091 total CD45+ cells were analyzed (2129 from NC, 3189 from WD and 2773 from WDDF).