title: Whole genome sequencing of Mycoplasma Pneumoniae : strain 6282
design: "Genomic DNA was collected using the Illustrabacteria genomic Kit (GE Healthcare) and sheared to 100 bp fragments using a Covaris S2 device. Paired-end Illumina libraries were created as described by Bentley et al. and the size selected to be between 200 and 400 bp. The resulting libraries were quantified on an Agilent Bioanalyzer chip (Agilent Technologies). Double-stranded templates were cluster amplified and sequenced on an Illumina GAII. Sequences were filtered according to quality, trimmed for the latest 5 bases ans subsampled to reach a coverage of about 150 times the genome of Mycoplasma pneumoniae M129"