title: GSM1199120: idm2_MethylC-Seq_rep2; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: idm2 samples: Libraries were prepared according to a published protocol (Lister et al., 2008). Briefly, Genomic DNA was extracted from 14-d-old seedlings. Sonicated DNA was end-repaired by using End Repair Enzyme Mix (NEB, #E6041A). The blunt end DNA were treated with Klenow fragment (NEB, #E6044A) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. Unmethylated cytosine residues were converted to uracil by EpiTect bisulfite Kit (Qiagen, 59104) after adaptor ligation. After bisufite treated DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of 250~350 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer IIx following the manufacturer's protocols in KAUST(King Abdullah University of Science and Technology) idm1idm2 sample: Genomic DNA was extracted from 14-d-old seedlings and sent to BGI (Shenzhen, China) for bisulfite treatment, library preparation, and sequencing. Bisulfite sequencing libraries were prepared using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM1199120