title: GSM2797291: Young_25_RNA; Homo sapiens; RNA-Seq
library_construction_protocol: HSCe RNA was extracted following the manufactures’ protocol for the Qiagen Allprep Micro Kit (#80284). Samples were eluted in 14 µL RNAse free water. An ethanol precipitation was then used to concentrate samples. Samples with a RIN>8.5 were used for library preparation. Ribosomal RNA was removed using RiboGone (Clontech, #634846). Stranded libraries were prepared by the University of Michigan Sequencing Core using the SMARTer Stranded RNA-seq Kit (Clontech, #634836). Libraries were sequenced on the HiSeq-2500 with 50 bp pair-end sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2797291