title: GSM2258246: Stress Female 1; Mus musculus; RNA-Seq
library_construction_protocol: NAc samples were collected and processed as described previously (Golden et al., 2013). Bilateral 14-gauge NAc dorsal striatum punches were collected on ice after rapid decapitation and then immediately placed on dry ice and stored at -80°C until use. RNA was isolated using homogenization in Trizol (Invitrogen) followed by chloroform layer separation. The clear RNA layer was then processed (RNeasy MicroKit, QIAGEN) and analyzed with NanoDrop (Thermo Scientific). A total of 500 ng of RNA was then reverse transcribed to cDNA (qScript Kit, VWR). Library construction was performed following a previously published protocol (Feng et al., 2014). Bilateral 14 gauge NAc dorsal striatum punches were collected on ice and stored as described above. RNA was purified with RNeasy Micro columns, and Bioanalyzer confirmed that the RNA integrity numbers were >8.0. Total RNA (4 μg) was used for mRNA library construction following instructions of Illumina mRNA sample prep kit (catalog #RS-100-0801). Libraries were created from pooled mRNA samples (n = 4 mice/library and run in independent biological replicates of 3 libraries per sex/stress).
attributes.tag: GEO Accession
attributes.value: GSM2258246