title: GSM1654760: si-Gcn5_D2_RNASEQ_2; Mus musculus; RNA-Seq
library_construction_protocol: 2o 1B MEFs (p5) were seeded as 1.2 x 106 cells on 0.1 % gelatin coated 15 cm plates and treated 24 hrs later with 1.5 μg/mL of Doxycycline (Dox) in mES media, while control cells were kept in mES media in the absence of Dox. The cells were collected after 2 days of Dox exposure and processed as previously described (Beyer et al. 2013), with the exception of chromatin sonication, where 10 x 20 pulses (25 % amplitude) of the Sonicator 4000 (QSonica) were used, and 15 μg of chromatin was precipitated with 1 μg of c-Myc antibody (sc-764, Santa Cruz). RNASeq Libraries were prepared according to the Illumina TruSeq Strandard Total RNA Sample Preparation Kit (with Ribo-Zero Gold, RS-122-2301), 10 pM of each library was sequenced according to standard Illumina protocols on an Illumina HiSeq 2000.
attributes.tag: GEO Accession
attributes.value: GSM1654760