title: GSM2261944: DS198; Homo sapiens; miRNA-Seq
library_construction_protocol: Cytoplasmic extracts were prepared as described previously (Sterne-Weiler et al. 2013; Martinez-Nunez and Sanford 2016) with the addition of 500g/mL cycloheximide (Sigma-Aldrich UK) in the lysis buffer. NEBNext Small RNA-Seq Library Preparation Kit (New England Biolabs- Ipswich, USA). The purified libraries were electrophoresed through a freshly cast 8% native polyacrylamide gel. The library fragments of appropriate size (~134-152 nt) were excised and recovered by agitation of the fragments overnight at 37°C at 200 rpm in elution buffer, passage of the eluate through a 0.45μm filter, and ethanol precipitation. miRNA-seq. Run across two lanes (L001 and L002).
attributes.tag: GEO Accession
attributes.value: GSM2261944