title: Aspergillus fumigatus CEA10 Transcriptome or Gene expression
design: cDNA samples were prepared for multiplexed, paired-end sequencing following the manufacturers protocol. For each isolate, 1-5µg cDNA in 200µl was sheared and then purified using the QIAquick PCR Purification kit (Qiagen). Sequencing libraries were generated using the ScriptSeq kit v2 (Epicenter) following manufacturer’s directions.  After each preparation step sample quality and quantity was assessed using Bioanalyzer and the Agilent RNA 6000 Nano Kit (Agilent).  All cDNA libraries were sequenced (4 samples per lane) using the Illumina HiSeq2000 instrument (www.illumina.com) at the Oregon Health Sciences University Massively Parallel Sequencing Shared Resource (http://www.ohsu.edu/xd/research/research-cores/mpssr/)