title: GSM1841544: sd_dil_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the somatodendritic and the axonal compartment using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) with 10 μl elution volume. For samples 1-34 and 38-43 cDNAs were first amplified using a double-random priming strategy followed by library construction with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Briefly, total RNAs was reverse-transcribed with an oligonucleotide used previously for whole genome amplification [multiple annealing and looping-based amplification cycles (MALBAC)] containing a 3' random octamer and a 5' adapter sequence. The same oligonucleotide was used for one round of second strand synthesis generating cDNA fragments that harbor the adapter sequence in a reverse-complementary manner at both ends. These fragments were then amplified by PCR using adapter oligonucleotides to produce sufficient cDNA amounts for generating high-throughput sequencing libraries. For samples 35-37 RNAseq libraries were generated with the NEBNext Ultra RNA Library Kit for Illumina (NEB) with omission of the mRNA isolation step.
attributes.tag: GEO Accession
attributes.value: GSM1841544