title: Human Lung Microbiome from patients with pneumonia
design: Sequencing libraries were generated and barcoded using Illumina’s Nextera DNA Sample Preparation Kit, and sequenced on a HiSeq 2500 instrument. This generated an average of 17.5 million single-end, 100bp sequencing reads per sample.  Lung aspirates were collected from 8 patients at The George Washington University Hospital with suspected ventilator-associated pneumonia (VAP) by endotracheal suctioning.