title: GSM4649147: HCT116: Human colorectal carcinoma cells-input DNA; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitation (ChIP) assays were performed using a ChIP assay kit (Millipore, Temecula, USA) according to the manufacturer's instructions. Briefly, cells were fixed with formaldehyde and homogenized. Nuclei were pelleted, and DNA was sheared by sonication and immunoprecipitated with anti-RUNX2 antibody or negative control immunoglobulin G using protein A/G beads. DNA was released from immunoprecipitate complexes using proteinase K and purified. Chromatin was sheered by 8 sonication cycles to generate DNA fragments with an average size of 200 bp for ChIP-seq analysis and 4 sonication cycles to generate DNA fragments with an average size of 600 bp for ChIP-qPCR. For high-throughput sequencing, 10-20 ng DNA was used to make the ChIP-seq library according to the instructions from Illumina's ChIP-seq Sample Prep kit. The ChIP-seq library was loaded on Illumina's cluster station and sequenced with Illumina Genome Analyzer IIx.
attributes.tag: GEO Accession
attributes.value: GSM4649147