title: GSM4714977: Sample48: E275_J2_IP_RIII_etoh_dsRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in the presence of NP-40. Lysates were treated with DNase I, RNase T1 and, where indicated, with RNase III. Double stranded RNA in the lysate was immuno-precipitated over-night using the J2 Antibody (Scicons). Following washing, RNA was extracted from the beads with TRIZOL and precipitated with isopropanol. Ribosomal RNA was depleted from the immuno-precipitated RNA using the NEBNext rRNA depletion Kit (New England Biolabs) and the rRNA depleted samples were further processed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Size-selection of libraries was performed using Agencourt AMPure XP Beads (Beckman Coulter). The quality, quantity and the size of the cDNA libraries were determined with the Fragment Analyzer (Thermo Fisher Scientific, previously Advanced Analytical Technologies). Libraries were sequenced on a NextSeq500 System (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4714977