title: Prolonged use of a proton pump inhibitor reduces microbial diversity
design: 50 ng of gDNA  was used as template for a polymerase chain reaction (PCR) with 0.3 uM V3-V5 barcoded primers targeting 357F and 926R (5’AATGATACGGCGACCACCGAGATCTACACTATGGTAATTGTCCTACGGGAGGCAGCAG3’ and 5’CAAGCAGAAGACGGCATACGAGATGCCGCATTCGATXXXXXXXXXXXXCCGTCAATTCMTTTRAGT3’) of the bacterial 16S gene.  PCR conditions were set to 95°C/3min, 35 cycles of 98°C/30s, 70°C/15s, 72°C/15s and finally 72°C/5 min in a Bio-Rad T100 Thermal Cycler.