title: GSM1841066: KO1 Neuron; Homo sapiens; RNA-Seq
library_construction_protocol: Briefly, tota cellularl RNA were extracted to produce cDNA using DT-T7 primers and the miRNeasy Kit (Qiagen) according to the manufacturer’s protoco RNA libraries were prepared for sequencing using standard Illumina protocols. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified cDNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM1841066