title: GSM4763880: Glands_5; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from: unprocessed gland elements (small aliquot collected from the sieve after filtering the initial digest of decidua), growing organoids and endometrial stromal fibroblasts isolated from the same samples and established as primary cell cultures using RNeasy Plus Mini kit (QIAGEN, 74134) following manufacturer's protocol, followed by DNase treatment using TURBO DNA-free Kit (Thermo Fisher Scientific, AM1907). RNA quality and quantity were assessed on 2100 Bioanalyzer (Agilent Technologies, Inc.). RNA-Seq libraries were prepared using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human (RS-122-2201, Illumina Inc.) and following manufacturer's protocol. Library quality and quantity were checked on 2100 Bioanalyzer and the pool of libraries was sequenced on Illumina HiSEQ4000 (single-end 50bp) using manufacturer's reagents and protocols. DNase treatment, quality control, Ribo-Zero library preparation and Illumina sequencing were performed at the Genomics Facility at The University of Chicago.
attributes.tag: GEO Accession
attributes.value: GSM4763880