title: SMRT-Seq of Dendrocalamus latiflorus: 1k-2k
design: Samples from five stages during bamboo shoot organogenesis were used for RNA extraction using RNeasy Plant Mini Kit (Qiagen, China) according to the manufacturers instructions. RNA samples from different tissues were mixed in equal amounts for further steps. The RNA was purified from 5 g of mixed total RNA using poly (T) oligo-attached magnetic beads, and the first-strand cDNA was synthesized using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, CA, USA). Remaining overhangs were converted into blunt ends by exonuclease/polymerase activities. After the large-scale PCR process, the full-length cDNA fragments were screened using BluePippin (Sage Science, Beverly, MA, USA) and cDNA libraries were generated according to the protocol.