title: E. coli gDNA amplified by DOP-PCR with primer GAGTGGGGTTGGGTTGGGGGGGGGNNNNNN with 5 low stringency (cold) followed by 30 high stringincy cycles on thermocycler 
design: "Stringent and non-stringent PCR cycles using the degenerate primer listed, genomic template DNA (0.2ng/ul) and NEB LongAmp Taq Polymerase. Randomized fragments were purified, sheared in a Covartis down to 150bp and sequenced"