title: GSM3580291: 102415-16; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from purified NK cells using the PicoPure® RNA Isolation Kit (Arcturus). To assess the quantity of the samples, the concentration of the RNA was determined using the Nanodrop ND1000 spectrophotometer. The quality and integrity of the RNA samples were analyzed on a RNA 6000 Lab-on-a-Chip using the Bioanalyzer 2100 (Agilent Technologies). mRNA was isolated from total RNA using the poly-T-oligo-attached magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. The sequence libraries were prepared using the TruSeq RNA sample preparation kit (Illumina) according to the manufacturer's protocol. The quality and yield after sample preparation were measured with a DNA 1000 Lab-on-a-Chip. The size of the resulting products had a peak around 300 basepairs on a DNA 1000 chip.
attributes.tag: GEO Accession
attributes.value: GSM3580291