title: GSM2262813: Donor D30, live sorted cells, library 6; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were sorted into 5µl Vapor-Lock (Qiagen) containing a droplet with primers and dNTPs. Cells were lysed at 65 degrees Celsius for 5 minutes. mRNA was then reverse transcribed by adding RT mix. RNA was processed by an automated version of the previously described Cel-Seq2 technique. Libraries were sequenced on a Illumina NextSeq500 using 75bp paired end sequencing.
attributes.tag: GEO Accession
attributes.value: GSM2262813