title: DNA-seq of mouse PDAC metastasis  organoid: mM28
design: The collected single nuclei were amplified using the SeqPlex Enhanced DNA Amplification kit (Sigma-Aldrich) following the published protocol. 1 ug of WGA product was then end-repaired, A-tailed, and ligated to TruSeq adapters (Illumina). The ligated DNA was purified using AMPure XP beads (Beckman Coulter), then pooled, amplified and quantified for sequencing using a Bioanalyzer 2100 (Agilent). The pools were sequenced on the Illumina NextSeq 500 instrument using single-read, 76bp reads.