title: RNA-Seq of Prokaryotes: Alkaline Hot springs
design: DNA extraction from environmental samples using phenol-chloroform  protocol, PCR amplification of 16S rRNA gene variable region (V4 – V7) and library construction following Illumina sequencing protocol. Barcode sequence, name, linker: GAGAGTGT; 515Fbar1; GTGCCAGCMGCCGCGGTAA GAGATCAG; 515Fbar2; GTGCCAGCMGCCGCGGTAA GAGATCTC; 515Fbar3; GTGCCAGCMGCCGCGGTAA GAGTACTC; 515Fbar7; GTGCCAGCMGCCGCGGTAA GAGTAGAC; 515Fbar8; GTGCCAGCMGCCGCGGTAA GAGTAGTG; 515Fbar9; GTGCCAGCMGCCGCGGTAA GAGATGAC; 515Fbar4; GTGCCAGCMGCCGCGGTAA GAGATGTG; 515Fbar5; GTGCCAGCMGCCGCGGTAA GAGTACAG; 515Fbar6; GTGCCAGCMGCCGCGGTAA