title: GSM3271528: 4128 YC GC cells; Mus musculus; OTHER
library_construction_protocol: Murine spleens were submitted to cellular disggregation, followed by lysis of erythrocytes using ACK buffer. Then, single-cell preparations were stained for B220, CD38 and Fas and submitted to fresh cellular sorting in a FACSAria Ilu sorter (BD) after exclusion of dead cells with 7AAD. Intracellular YFP or GFP expression results from CRE-mediated recombination of corresponding reporter alleles at the ROSA26 locus of YC or BIC/pBIC mice, respectively. Total RNA was extracted from sorted cells using TRIzol (Invitrogen) as described in manufacturer´s protocol and quality control of extracted RNA was measured using Agilent Bioanalyzer. As described in Vergani et al. Front Immunol 8, 1157 (2017), with specific murine primers indicated in Supplemental Information of the publication associated with this dataset.
attributes.tag: GEO Accession
attributes.value: GSM3271528