title: GSM4462450: Nalm6-DMSO scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: scRNAseq: Nalm-6-RUNX1 was induced for 72 h using 500 ng/ml doxycycline (Sigma-Aldrich) before scRNA-seq cell collection. Excess dead cells were depleted from samples using bead-based Dead Cell Removal Kit (#130-090-101, MACS miltenyi Biotech) scRNAseq: Cells were combined with reverse transcriptase Master Mix and partitioned into Gel Bead-In EMulsions (GEMs) using 10X GemCode Technology, where the poly-A transcripts are barcoded with an Illumina R1 sequence, a 16 bp 10X barcode and a 10 bp Unique Molecular Identifier (UMI). 11-12 cycles of PCR was used to amplify the cDNA.
attributes.tag: GEO Accession
attributes.value: GSM4462450