title: GSM3660740: ChIP-seq.NELFA_plusDox_rep2_HAantibody1; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP-seq: Dox-induced were fixed with formaldehyde (final 1%) for 10 minutes. Fixed cells were then subjected to standard ChIP lysis protocol to release chromatin. Released Chromatin was sonicated for 15 cycles (30 sec on, 30 sec off) on a Biorupter at high setting. Soluble chromatin was collected and between 1-2 mg was used for IP with 4-8 ug of respective Dyna-protein G conjugated antibody complex. Captured fragments were subjected to several rounds of stringent salt washes before eluting with SDS buffer. ChIP-seq: Eluted DNA was purified and subjected to NGS library preparation using the NEBNext DNA ultra II kit. Constructed ChIP libraries were sequenced on the Illumina NextSeq or HiSeq platform.
attributes.tag: GEO Accession
attributes.value: GSM3660740