title: 16S rRNA V4 from coral reef water  (SampleID: O_S28): With peptide nucleic acid clamp
design: Microbial DNA extracted from 1L of sea water. Filtered first through a prefilter and then through a 0.22um Sterivex filter. DNA extracted using Qiagen PowerSoil kit following manufactuer protocols. Amplified using 515F-806R primers for 16S rRNA (V4 region). PNA clamp designed in Reigel et al. 2020 was added to the PCR reaction.