title: Illumina of sludge samples: SCR PN-AA textile
design: 16S NGS analysis  was performed at Stab Vida Lda (Lisbon, Portugal). Sequencing targeted the V5  and V6 regions (250bp) of the 16S rRNA gene using the primers 799F  (5-AACMGGATTAGATACCCKG-3) and 1175R1 (5-ACGTCRTCCCCDCCTTCCT-3) (Bonder et al.  2012). Samples were then sequenced with MiSeq Reagent Kit Nano though the  Illumina MiSeq platform.