title: Cryphonectria parasitica transcriptome or gene expression
design: RNA samples with concentrations around 60 ng/ul and total amounts ranging 1-2 ug were submitted to sequencing facility in IBBR, MD.  Samples included three biological replicates of DK80, ?rdr12, ?rdr123 (C1) and one ?rdr4.  RNA quality check was done by electrophoresis run using Bioanalyzer (Agilent) RNA chip, resulting in RIN values between 8.8-10.  Illumina libraries with Illumina adaptors and indexes were generated using Illumina TruSeq RNA Sample Preparation kit v2 by following the kit manual. After the construction of cDNA library, Bioanalyzer (Agilent) DNA chip was used to perform quality check. The sequencing-ready library at 2 nM was followed by cluster generation using Illumina cBot, and loaded on Illumina HiSeq 1000 for sequencing. The desired library insert size was set at 200-300 bp, paired-end read length at 100 bp.  