title: RNA-seq of Pimephales promelas: Pooled whole organisms, unfertilized eggs to 30 days post hatch
design: RNA isolated  from 12 immature development stages; unfertilized eggs (3 replicate isolations ("reps") of 30 eggs each), 24 hours post-fertilization ("hpf", 2 reps of 30 eggs each), 48 hpf (2 reps of 30 eggs each), and 72 hpf (2 reps of 30 eggs each) , newly hatched larvae (3 reps of 15 larvae each), 24 hours post-hatch ("hph", 3 reps of 15 larvae each)  48 hph (3 reps of 10 larvae each) , 72 hph (3 reps of 10 larvae each), and 96 hph (3 reps of 10 larvae each), and 7 days post-hatch ("dph",  3 reps of 8 larvae each), 17 dph (3 reps of 4 larvae each) and 30 dph (5 reps of individual larvae). Equal amounts of total RNA from one replicate isolation from each stage, except 30 day post-hatch larvae, where all 5 replicates were used to make one pool, were combined to make final pool for sequencing.