title: GSM4151326: ATAC_Eomes_null_rep_1; Mus musculus; ATAC-seq
library_construction_protocol: Tagmentation and indexing of single cell suspensions (6x104 cells) from three independent differentiations of WT (CCE) and Eomes-null (clone 6A6, CCE) day 4 Flk-1hi/PdgfRa- cells was performed as previously described(Buenrostro et al., 2013), using cells from equivalent samples to those used for the RNA-seq experiments described above. To control for sequence bias of the Tn5 transposase, 100ng genomic DNA of WT (CCE) ESCs was also tagmented and indexed. Libraries were purified with two rounds of Agencourt AMPure XP bead cleanup (Agencourt, 1.5× bead:sample ratio). Library size and concentration were determined using the 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent). Samples were sequenced using a 75-cycle paired end Nextera kit with custom Nextera index primers taken from Table S1 in Buenrostro et al. 2013(Buenrostro et al., 2013) on the Illumina HiSeq4000 platform.
attributes.tag: GEO Accession
attributes.value: GSM4151326