title: RNA-Seq of mus musculus
design: For isolation and purification of RNA, the Trizol method (Ambion Life Technologies, Carlsbad, CA) and the Direct-zol RNA kit (Zymo Research, USA) were used. To remove unwanted DNA, purified RNA was treated with DNAse for 30 min at room temperature according to the manufacturers instructions. RNA quantity was measured by Qubit RNA libraries were prepared for sequencing using standard Illumina protocols