title: Illumina HiSeq 3000 sequencing; RNA-seq of mouse heart that is both heart SOD2 knockout and deficient for mitochondrial DNA repair
design: RNA-seq of mouse heart that is both heart SOD2 knockout and deficient for mitochondrial DNA repair
library_construction_protocol: Heart tissue was rinsed in PBS to remove excess blood and snap-frozen in liquid nitrogen directly after dissection. RNA was extracted from snap-frozen heart tissue with TRIzol (Ambion) following manufacturer's recommendations with an overnight isopropanol precipitation at -20°C. The quality of RNA was verified by  resolving it on a formaldehyde-agarose gel prior to library preparation rRNAs were depleted with RiboZero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) following manufacturer's recommendations with an input of 1 µg total RNA. Next, the RNA library was prepared with NEBNext Ultra Directional RNA Kit (New England Biolabs) following manufacturer's recommendations
attributes.tag: Experimental Factor: phenotype
attributes.value: Ckmm driven knockout of Sod2