title: Illumina sequencing of Human adenovirus 21a
design: Total DNA was sheared into ~350-bp fragments by the Covaris ultrasonicator (Covaris, Woburn, MA, USA). A-tailed, ligated to paired-end adaptors and PCR amplified with a 350 bp insert was used for the library construction. Qubit 2.0 (Life Technologies, Carlsbad, CA, USA) was then used to quantify the concentration of the library and the insert size tested by Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Illumina HiSeq PE150 platform (Illumina, San Diego, CA, USA) was used to generate 2  150-bp pair-end reads.