title: GSM3268048: DSS-rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted by TRIZOL reagent according to the manufacturer's instructions (Invitrogen, USA). The quality and quantity of total RNA were assesses using 2% agarose gels and Qubit® 3.0 fluurometer (Life Technologies, CA, USA). The concentration and integrity of RNA were assessed using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB E6111). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM3268048