title: amplicon sequencing from red clover_Bacteria_Rhizosphere_grassland_future climate_Replica9
design: The V5  V7 region of the bacterial 16S rRNA was amplified using the forward primer BAC799F (5-AACMGGATTAGATACCCKG-3) and the reverse primer BAC1193r (5- ACGTCATCCCCACCTTCC -3)