title: GSM4471373: WT-input-rep3; Mus musculus; ChIP-Seq
library_construction_protocol: The mice were treated for 5 consecutive days with tamoxifen, by intraperitoneal injection. After 7 days, muscle regeneration was induced with cardiotoxin (CTX), via intra-muscular injection. Muscles and FAPs were then isolated 5 days post-injury (d.p.i). FAPs were expanded on ECM-coated dishes for 4/5 days and were cross-linked with 1% formaldehyde. Chromatin sonicated to an average size of 200-150 bp. Chromatin was precipitated with specific antibody, which was further purified using magnetic protein A/G coupled beads, and recovered DNA was used for high-throughput sequencing. DNA libraries were prepared with 2–10 ng of DNA using an in-house protocol (Blecher-Gonen et al., 2013) by the IEO genomic facility.
attributes.tag: GEO Accession
attributes.value: GSM4471373