title: GSM4466631: WT-1h; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs from BV-untreated and –treated iPSCs were extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. After quality assessment of the RNA by 2100 Bioanalyzer Instrument (Agilent, Santa Clara, CA, USA), only samples with an RNA integrity number >7.0 were included in the QuantSeq analysis. From 500 ng total RNA, double-stranded cDNA libraries were prepared using a TruSeq Stranded Total RNA LT Sample Prep Kit with Rico-zero Gold (Illumina, San Diego, CA, USA). The sequencing library is prepared by random fragmentation of the cDNA sample, followed by 5' and 3' adapter ligation. Adapter-ligated fragments are then PCR amplified and gel purified.
attributes.tag: GEO Accession
attributes.value: GSM4466631