title: GSM3660748: H3K27me3_BC_rep2; Malus domestica; ChIP-Seq
library_construction_protocol: Preparation of chromatin samples and immunoprecipitation were performed as described previously (Gendrel et al., 2005). Three to four flower buds were used as one replicate. Cross-link was conducted as described previously (Saito et al., 2015) with minor modifications. In brief, each flower bud was first cut longitudinally by razor and divided into 8 equal parts. The immunoprecipitated chromatin samples were amplified and used for library construction using the ThruPLEX DNA-seq Kit (Takara Bio USA) as per the manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM3660748