title: mNGS of Sacral abscess
design: Take 1mL of pus sample, add 50uL proteinase K and 100uLSDS, lyse in a constant temperature metal bath at 60C for 20min, after the reaction, take 200uL sample and extract DNA with QIAamp DNA Mini Kit. The extracted DNA is interrupted by ultrasonic to 200-500bp, and then Ion Plus Fragment Library Kit (Life Technologies) is used to construct a DNA library. The built library uses 2100 quality inspection fragments. Finally on the machine at proton.