title: GSM4212557: Aortic cells, 1_1; Mus musculus; RNA-Seq
library_construction_protocol: Aorta was collected and digested for 1 hour at 37°C in an enzymatic mix (10 mg/ml Collagenase type II (C6885, Sigma Aldrich) and 1mg/ml Elastase (LS002292, Worthington Biochemistry). Digested aortas were mechanically disrupted and immediately processed for single-cell RNA-sequencing. The cellular suspensions were loaded on a 10x Genomics Chromium instrument to generate single cell gel beads in emulsion (GEMs) Libraries were prepared using the following kits: Chromium Single Cell 3' Library & Gel Bead Kit v2, PN-120237; Single Cell 3' Chip Kit v2 PN-120236 and i7 Multiplex Kit PN-120262, 10x Genomics) Sequencing was performed on an Illumina HiSeq 4000 as 2 x 150 paired-end reads, one full lane per sample, for approximately >90% sequencing saturation. For alignment, barcode assignment and unique molecular identifiers (UMI) counting, the Cell Ranger Single Cell Software Suite, version 1.3 was used to perform sample de-multiplexing, barcode and UMI processing, and single-cell 3′ gene counting (https://support.10xgenomics.com/single-cell-gene)
attributes.tag: GEO Accession
attributes.value: GSM4212557