title: GBS of Medicago sativa: PI464713_3_
design: We constructed libraries for genotyPIng-by-sequencing (GBS). Each library Was 96-plexed With 5-9 individuals per population. Libraries Were generated folloWing the protocol of Elshire et al. (2011) With minor modifications as described by Li et al. (2015). We used the frequent cutting enzyme ApeKI and sets of 384 barcodes (4-8-bp) for multiplex sequencing. Sequences Were obtained With an Illumina HiSeq 2500 system that generated 100-bp single-end reads.