title: 16S rRNA sequencing: fecal sample healthy individual
design: Partial 16S rRNA gene sequences were amplified using the Ion 16S Metagenomics Kit (Thermo Fisher) using specific primers (Primer set V2-4-8; primer set V3-6, 7-9) for hypervariable regions. Library creation was performed using Ion Plus Fragment Library kit, following manufacturers in-structions (Thermo Fisher). Template preparation and sequencing was performed with Ion 510 & Ion 520 & Ion 530 Kit Chef, in an Ion Chef Instrument and an Ion GeneStudio S5 sequencer respectively, according to manufacturer's procedures (Thermo Fisher). Two NGS runs were conducted using Ion 520 semi-conductor chips, one chip for each group of study. We used an 850-flow runs, which sup-port a template read length of 400 bp average.