title: GSM2870562: Saline-treated F2 female brain Xist Δ/Δ rep2 (N11-2); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol from tissue that was snap frozen in liquid nitrogen and grinded. Ribosomal RNA was removed using RiboMinus. Strand-specific libraries were generated by performing reverse transcription with Superscript III, followed by second-strand synthesis using the NEBNext second-strand synthesis module, and then libraries were generated using the NEBNext ChIP-seq Library Preparation Kit.
attributes.tag: GEO Accession
attributes.value: GSM2870562