title: RNA-seq of malaria liver hypnozoites
design: Total RNA samples extracted from FACS-sorted small parasite infected cells (e.g. hypnozoites) and FACS-sorted large parasite infected cells (e.g. liver schizonts) were amplified using the TargetAmp™ 2-Round aRNA Amplification Kit 2.0 (Epicentre). RNA-seq cDNA libraries were prepared from the amplified RNA samples using the TruSeq mRNA Sample Prep kit v2 (Illumina). RNA-seq cDNA libraries were then sequenced in paired-end mode, 2x76 bp, using the Illumina HiSeq2500 platform.