title: Illumina MiSeq paired end sequencing; Small RNA discovery in developing mouse and during human in vitro beta-cell differentiation protocol
design: Small RNA discovery in developing mouse and during human in vitro beta-cell differentiation protocol
library_construction_protocol: Small RNA's were size fractionated from total RNA based on charge. Capped transcripts were enriched by immunoprecipitation and poly-A selected with Oligo dT. An enzymatic cocktail was applied to modify the RNA's for use with the NEBNext Kit. Please contact the investigators for further information. NA
attributes.tag: Experimental Factor: organism part
attributes.value: brain
attributes.tag: Experimental Factor: stage
attributes.value: not applicable