title: GSM4151572: Input; Homo sapiens; ChIP-Seq
library_construction_protocol: For RNA-seq, RNA were extracted with RNAeasy midi kit (Quiagen). Library preparation and sequencing was done with the kit TruSeq Stranded total RNA according to mmanufacturer's institutions. Sequencing was done with HiSeq3000-HWI-J00115 according to the manufacturer's protocol. For ChIP-seq, cells were cross-linked with PAF.  Chromatin was sheared using a Bioruptor (Diagenode)  (30 cycles, 30 sec ON/ 30 sec OFF).  Proteins were immunoprecipited with antibodies. After RNA and protein degradation, DNA was extracted with a phenol-chloroform extraction. Sequencing was done HiSeq3000-HWI-J00115 according to the manufacturer's protocol.
attributes.tag: GEO Accession
attributes.value: GSM4151572