title: 16S rRNA gene sequencing of microbial community: samples from Clarion-Clipperton Zone
design: Amplified using primers 515F-Y and 926R (Parada et al. 2016 doi:10.1111/1462-2920.13023) with attached Illumina adapters following the Earth Microbiome Project. Cleaned with ENZA Cycle Pure Kit and pooled at equimolar proportions. Paired-end 250 (v2 chemistry) sequencing conducted at University of Montana.