title: GSM2449155: 1M1; Rattus norvegicus; RNA-Seq
library_construction_protocol: At 1 month, 3 months, and 6 months post-SCI, animals were intracardially perfused with 0.01M phosphate-buffered saline under anesthesia. A segment of the spinal cord tissue (0.5 mm) at the epicenter was dissected from each animal and snap-frozen in liquid nitrogen. Animals in the control group were sacrificed at 6 months post-SCI and a section of the spinal cord tissue at an equivalent location was collected. Total RNA was extracted from the spinal cord tissue using TRIzol reagent (Invitrogen) following the manufacturer’s instructions.  RNA quality was determined using a Bioanalyzer (Agilent), and all RNA integrity numbers (RINs) were greater than 8.  About 150–300 ng total RNA was used to construct each RNA-Seq library. RNA samples were poly-A selected and paired-end sequencing libraries were constructed using the TruSeq RNA Sample Prep Kit, as described in the TruSeq RNA Sample Preparation V2 Guide (Illumina). Samples were then sequenced using the Illumina HiSeq 2000 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM2449155