title: 16S metagenomic sequencing of mock community:  Mock community positive control 4
design: A 50ul sample was taken from a mock community consisting of a 1:5 mixture of Staphylococcus aureus, Lactococcus lactis, Porphyromonas gingivalis, Streptococcus mutans, and Escherichia coli.  Total genomic DNA was extracted from the nasal samples using a modification of the ZymoBIOMICSTM DNA Miniprep Kit (Zymo Research, Irvine, CA) and FastPrep mechanical lysis (MPBio, Solon, OH).  16S ribosomal DNA (rRNA) was amplified with Phusion High-Fidelity polymerase (Thermo Scientific, Waltham, MA) and dual indexed primers specific to the V1-V3 (8F: 5 AGAGTTTGATCCTGGCTCAG 3;  534R: 3 ATTACCGCGGCTGCTGG 5) hypervariable regions. Amplicons were pooled and paired-end sequenced on an Illumina MiSeq (Illumina, San Diego, CA) in the University of Rochester Genomics Research Center.