title: GSM4388164: sgRosa_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: MOLM13 cells were transduced with two independent sgRNAs targeting ZFP36L2 and compared to non-targeting sgRNA (sgRosa). After 6 days post-transduction, cells were washed with 1x PBS and total RNA was extracted using Rneasy Plus Mini Kit (Qiagen) and QIAshredder, which were performed according to manufacturer's protocol. For poly(A) mRNA isolation, magnetic isolation (NEB) was performed using ~1ug of total mRNA. RNA-seq libraries were generated using NEXTflex Rapid Directional (Bioo Scientific) protocol and NEXTflex RNA-seq barcodesand sequenced using Hi-Seq4000 (50 cycles single end)
attributes.tag: GEO Accession
attributes.value: GSM4388164