title: GSM1195958: Aneuploid_trisomy13; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of each line was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Poly(A)+ mRNA was isolated from total RNA using Oligotex (QIAGEN). RNA was fragmented with RNase III, preparing for constructing transcriptome libraries of each iPSC cell line. Applied Biosystems SOLiD Whole Transcriptome Analysis Kit ( http://solid.appliedbiosystems.com) were applied to perform reversed transcription from 140–200 bp isolated RNA fragments into Single-strand cDNA.
attributes.tag: GEO Accession
attributes.value: GSM1195958