title: Bacterial diversity in soils from high-altitude lakes located in the Southern Bolivian Altiplano
design: The total DNA was amplified with 16S rRNA gene universal Bacterial primer 27F (27F 5_-AGRGTTTGATCMTGGCTCAG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix Kit (Qiagen, Valencia, CA).