title: GSM4715026: Neurosphere culture-Male; Mus musculus; RNA-Seq
library_construction_protocol: Confluent neurospheres were trypsinized using 0.25% EDTA‐free trypsin (Thermo Fisher; Catalog # 15090046) for 5 minutes at room temperature with occasional pipetting. Trypsin was subsequently inactivated with 10% FBS, and cells were filtered through a 40‐micron filter to ensure single‐cell suspensions. The cells were then spun down followed by an ice‐cold PBS wash, and 10,000 cells were reserved for ice‐cold methanol fixation.  We used the Chromium Single Cell 3′ Reagent Kit (10X Genomics, Pleasanton, CA; CG000183) to prepare a single‐cell master mix using an input of 7,000 rehydrated cells. The single‐cell master mixes were subsequently loaded into Chromium Chip B (10X Genomics; PN‐1000074), and gel beads in emulsion were prepared according to the manufacturer's protocol followed by RT, cDNA amplification, and library preparation step. 
attributes.tag: GEO Accession
attributes.value: GSM4715026