title: GSM2260334: FCRL5+#2; Mus musculus; RNA-Seq
library_construction_protocol: Class-switched B cells (IgM- IgD- CD19+) that were either FCRL5+ or FCRL5- were isolated from the blood of P. chabaudi-infected mice by double-sorting on a FACS AriaII.  Total RNA was isolated, converted to cDNA, and pre-amplified as described in the SMARTer Ultra-low input kit (Clontech PN 634833) but with modified procedures for low cell number analysis (Fluidigm) RNA libraries were prepared for sequencing following Illumina protocols.  cDNA libraries were diluted to 0.25ng/ul, fragmented with the Nextera XT DNA kit (Illumina PN FC-131-1024), multiplexed, and sequenced on a Hi-Seq2500
attributes.tag: GEO Accession
attributes.value: GSM2260334