title: RNA-seq of medaka testis
design: For lncRNA sequencing, the ribosomal RNAs were removed from testis RNA pool (5.0 g) and ovarian RNA pool (5.0 g) according to the manuscript of the Ribo-Zero? rRNA Removal Kit (Illumina, San Diego, USA), respectively. The remaining RNAs were used to construct sequencing libraries by TruseqTM RNA Sample Prep Kit (Illumina, San Diego, USA) according to the product instruction. Finally, the testis library and ovary library were sequenced using Illumina HiSeq4000 for 2 *150 bp pair-end (PE) sequencing.