title: Datisca glomerata root nodule associated bacteria_16S_Davis CA USA source
design: Raw reads of two biological replicates of total root-nodule DNA from host Datisca glomerata (DgUCD1, DgUCD2) were obtained by 454 sequencing.  DNA fragments were amplified with 16S primers (forward AGAGTTTGATCCTGGCTCAG, reverse TGCTGCCTCCCGTAGGAGT), barcodes were added, and sequencing was carried out using 454 GS FLX Titanium technology. Sequences were filtered only for 0 barcode mismatches.