title: GSM4255959: mESCs, smallRNA_KO_d12_2; Mus musculus; miRNA-Seq
library_construction_protocol: Small RNA extraction following 0, 4, 8, 10 and 12 days of 4-OHT treatment was performed using the Qiagen miRNEasy Mini Kit according to manufacturer instructions. Small RNA sequencing libraies were prepared using Illumina TruSeq samll RNA library prep kit and sequenced in a Illumina 2500. Sequencing adapters were removed from fifty nucleotides long single-end reads using cutadapt (v1.8) and reads  aligned to the mouse genome (mm10) using bowtie2. The levels of miRNA ( miRBase v21) were quantified using HTseq.
attributes.tag: GEO Accession
attributes.value: GSM4255959