title: GSM4256057: P7 CTL1; Mus musculus; RNA-Seq
library_construction_protocol: C57BL/6 mice were purchased from the Model Animal Research Center of Nanjing University. The mice were maintained under specific pathogen-free (SPF) conditions at the Center for New Drug Safety Evaluation and Research, China Pharmaceutical University. The Uxt conditional knocking-out mice were generated by mating between Chx10-Cre and Uxtflox/flox mice. Chx10-Cre mice were purchased from Shanghai Biomodel Organism. Uxt flox mice were generated with CRISPR/Cas9-mediated homologous recombination of a 2289bp double stranded DNA donor containing two loxP sites flanking exon 3. Total RNA were extracted with Trizol reagent (Invitrogen). Total RNA was extracted from control and Uxt CKO retinas using the Trizol reagent (Invitrogen) and prepared into cDNA library according to the standard Illumina RNA-seq instruction. A cDNA library was generated from polyA+ RNA according to the Illumina RNAseq protocol. The cDNA library was sequenced on an Illumina Hiseq2500 by a 2X150 bp run.
attributes.tag: GEO Accession
attributes.value: GSM4256057