title: GSM4712997: RC2 Roots; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Galls and control roots were collected at 3 days post infection and frozen on liquid nitrogen. Total RNA was extracted using the AllPrep DNA/RNA/miRNA Universal Kit from Qiagen with several adaptations (Silva et al., 2019; doi: 10.3389/fpls.2019.00657) The libraries were prepared using the NEXTflex Bisulfite-Seq Library Prep Kit (BiooScientific), following the manufacturer's instructions. The fragment size distribution of the libraries were checked in the Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit. The libraries were then sequenced in one lane of an Illumina HiSeq 4000 PE100 platform. Two of the libraries (RC2 and RC3) were sequenced in an Illumina® HiSeq X PE150 (San Diego, California, USA). Both steps were performed by the company AllGenetics & Biology SL.
attributes.tag: GEO Accession
attributes.value: GSM4712997