title: GSM4152267: rad53-1 pol3-01 +HU 45min BrdU-IP-ssSeq; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: the collected samples were incubated with 1% fresh-made formaldehyde at 25°C for 20mins, and quenched with 0.125M Glysine for 5mins.  The harvested cells were lysed with glass beads, and the chromatin pellet were washed and sonicated. The average DNA fragment size is about 200-400bp.  Sheared chromatin was immunoprecipitated with an protein-specific antibody. The chromatin was reverse-cross-linked and immunoprecipitated.  DNA was exacted with the Chelex-100 method ssDNA library preparation method was modified from previous one (Meyer et al., 2012). To preserve the strand information, we use a specific RNA ligase, which also can ligated the ssDNAs in a sequence-independent way. The ssDNA library strand information was kept by sequential 3' end and  5' end ligation.
attributes.tag: GEO Accession
attributes.value: GSM4152267