title: MIDAS alternate protocol (aphidicolin 0.4 M) of U2OS cells.
design: MIDAS alternate protocol. Unsynchronized  U2OS cells were treated for 8 h with aphidicolin (0.4 M) and nocodazole (100 ng/ml). Mitotic cells were then isolated and incubated 4 h with EdU (10 M)  and HU (2mM). The cells were finally collected and the EdU-labelled DNA was isolated and sequenced.