title: Illumina HiSeq 2500 paired end sequencing; Proteotranscriptomic view on the role of spider silk glands during the silk spinning process
design: Proteotranscriptomic view on the role of spider silk glands during the silk spinning process
library_construction_protocol: A total of 10 N. clavipes spiders were collected in a georeferenced site from the campus of the University of São Paulo State at Rio Claro, SP, Southeast Brazil. The silk glands were dissected and stored in the presence of RNAlater stabilization reagent (ThermoFisher Scientific)  for RNA preservation until use. cDNA fragments subsequently were through an end-repair process, the addition of a single A base and ligation of the adaptors. The products were purified and enriched with 15 cycles of PCR (with a size distribution of ~200–250 bp) to create the final cDNA library.
attributes.tag: Experimental Factor: 1
attributes.value: individual