title: Ion Torrent Proton sequencing
design: Single-end sequencing of DNA sample isolated from microbiological community inhabiting weathered Kupferschiefer black shale, fragmented by sonication, ligated with sequencing adapters and amplified.
library_construction_protocol: Isolated DNA was used to prepare barcoded library with Ion Xpress™ Plus Fragment Library Kit (Thermo Fisher) according to manufacturer's manual. Library was subjected to clonal amplification on Ion One Touch 2 system (Thermo Fisher) using Ion PI™ Template OT2 200 Kit v2 (Thermo Fisher) and sequenced on Ion Proton sequencer using Ion PI™ Sequencing 200 Kit v2 (Thermo Fisher) according to manufacturer's manual.