title: GSM4255935: Nr4a1pos_Male2_Rep2; Mus musculus; RNA-Seq
library_construction_protocol: PND3 cells from Nr4a1-EGFP heterozygous mice were obtained from a time set  homozygote Nr4a1-EGFP and WT breedings using FACS cell sorter. The collected cells were subject to  RNA extraction. RNA was isolated using PicoPure RNA Isolation kit, which included a DNase treatment  step. RNA libraries were prepared for sequencing using the standard protocol from Takara Clontech Laboratories SMARTer Stranded Total RNA-Seq Pico Kit (Cat#635005 Clontech) using 10ng total RNA. RNAseq libraries were analyzed on an  Agilent High Sensitivity D1000 TapeStation, and quantification of the libraries were performed using the  KAPA Library Quantification Kit.
attributes.tag: GEO Accession
attributes.value: GSM4255935