title: 16S rRNA of mus musculus intestine: FMT transplanted 7 day old pup
design: DNA was extracted from up to 250 mg of whole intestinal tissue. The intestinal tract from the duodenum to the rectum was aseptically excised, cleaned of mesentery and placed in a sterile, pre-tared microfuge tube. Tissue was partially homogenized with autoclaved scissors, and DNA from up to 250 mg of sample was immediately extracted using the MoBio/QIAamp PowerFecal kit according to the manufacturers instructions or kept at 80 C until further processing. DNA was quantified using a NanoDrop, and samples were diluted to 2.5 ng l1 in MQ water. Sequencing libraries were generated by amplifying the V4 region of the 16S rRNA gene using barcoded primers. The whole PCR reaction was run on 1.0% agarose/TBE gel and bands were extracted using QIAGEN QIaquick gel extraction kit.