title: Sequencing of fungal ITS rRNA from soil samples collected near and distant from subsurface drip irrigation emitter, one system installed in 2012 and the other in 2016.
design: Total DNA was extracted from 0.25g of soil from each experimental microcosm (n=60) using a PowerSoil extraction kit. DNA was amplified for the Bacterial 16S rRNA subunit (regions V1-V3) using primers 27F (Lane 1991) and 519R (Lane et al 1985). Sequencing libraries were constructed following the BASE protocol (Bisset et al 2016).