title: Poly-A+ RNA-seq of A549 lung cancer cell line with no treatment, Replicate 1
design: Total RNA was extracted before and after combined treatment and checked for integrity by microcapillary electrophoresis on 2200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA). Poly-A+ RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on the HiSeq2500 platform in a 2 x 150-cycle run, at Biodiversa Srl (Trento, Italy). Three independent replicates were sequenced for each condition (untreated and treated) for both cell lines. After fastq quality control by using FastQC tool, raw reads were trimmed to 100 bp.