title: GSM4150423: S1117358_2: WT_APH_4; Homo sapiens; RNA-Seq
library_construction_protocol: SC-derived clusters at d27 were dissociated into single cells using TrypLE Express. The resulting dissociated cells were filtered through a 30 µm cell strainer and collected by centrifugation at 400 x g for 5 min. The cell pellet was resuspended in 5 mL 1X PBS containing 0.04% BSA and filtered through a 30 µm strainer. Cells were collected by centrifugation at 400 x g for 5 min and the pellet was resuspended with 1X PBS+0.04% BSA to a final concentration of 400-500 cells/µL. RNAseq libraries were prepared using Chromium Signle Cell 3' Library, Gel Breads & Muliplex Kit (10X Genomics)
attributes.tag: GEO Accession
attributes.value: GSM4150423