title: Diversity of attached and free-living bacteria in a temperate coastal lagoon
design: Water was collected collected approximately every two weeks.  Water samples were prefiltered on a 3 micron pore size filters and then on 0.22 micron sterivex. DNA was extracted using a modified saline extraction protocol and PCR amplification targeting the 16S rRNA gene was performed. 454 pyrosequencing was the final step in the sample processing.