title: GSM3579848: goat_low_muscle3; Capra hircus; miRNA-Seq
library_construction_protocol: Three adult females (~3 years old) from each of the low-altitude goat and Tibetan goat populations residing at distinct altitudes in southwest China (600 m and 3000 m) were used in this study. Animals were fed with free access to food and water, and killed humanely to ameliorate suffering. All of the animals procedures complied the guidelines for the care and use of experimental animals established by the Ministry of Agriculture of China. Six typical hypoxia-sensitive tissues (heart, kidney, liver, lung, longissimus muscle and spleen) were rapidly excised from the carcass, immediately frozen in liquid nitrogen, and then stored at −80 °C until RNA isolation. Small RNA libraries were constructed using the Illumina TruSeq Small RNA Sample Prep kit. Libraries were assessed using the Agilent 2200 TapeStation and sequenced on the Illumina HiSeq 2500 platforms.
attributes.tag: GEO Accession
attributes.value: GSM3579848