title: GSM1655099: placebo_G864_ATACseq; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was extracted from mouse brain samples Libraries were amplified for a total of 10–12 cycles, purified using 1.2X Ampure beads and eluted in 20ul H2O. Following purification, library fragments were amplified using 1x NEBnext PCR master mix and 1.25 μM of custom Nextera PCR primers. To reduce GC and size bias in our PCR we monitored the PCR reaction using qPCR in order to stop amplification prior to saturation. The libraries were amplified for 5 cycles, a 5ul aliquot was run in a qPCR side reaction for 20 cycles, to determine the additional number of cycles needed for the remaining 45 μL reaction. The libraries were purified using 1.2X Ampure beads and eluted in 20ul H2O. Libraries were amplified for a total of 10–12 cycles.
attributes.tag: GEO Accession
attributes.value: GSM1655099