title: GSM4467330: Δghk replicate 2; Streptococcus gallolyticus UCN34; RNA-Seq
library_construction_protocol: At DO(600)=0,5, RNA were extracted with the MP Biomedicals™ FastRNA™ Pro Blue Kit and treated with DNase (Invitrogen™ Ambion™ TURBO DNA-free Kit) to remove residual DNA. DNase was inactivated with the DNase inactivation reagent present in the kit. rRNA were depleted from 0,5 μg of total RNA using the Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina. Sequencing libraries were constructed using the TruSeq Stranded mRNA Sample preparation kit (20020595) following the manufacturer's instructions (Illumina). The directional libraries were controlled on Bioanalyzer DNA1000 Chips (Agilent Technologies) and concentrations measured with the Qubit® dsDNA HS Assay Kit (ThermoFisher).  Sequences of 65 bases were generated on the Illumina Hiseq 2500 sequencer.
attributes.tag: GEO Accession
attributes.value: GSM4467330