title: Whole genome sequencing of Spiroplasma citri strain C189
design: S. citri strain C189 was isolated from citrus near Riverside, CA in the 1960s and maintained in culture. For sequencing, S. citri strain C189 was triple-cloned and cultured in LD8 medium to obtain enough DNA for sequencing. Total genomic DNA was extracted using CTAB method and library was prepared for sequencing using PacBio SMRTbell Express Template Prep Kit. Whole-genome sequencing was performed on the PacBio RS II platform. Reads were filtered and assembled using SMRT Analysis software from PacBio with default settings.