title: GSM905447: ES cell d7 H3K4me3 ChIP-seq; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP was performed as described in Lee et al. Nat Protoc. 2006;1(2):729-48. Paired-end Solexa ChIP libraries were prepared as described in Illumina ChIP sequencing manual with minor modifications and using NEB Next DNA sample prep reagent Set 1 (E6000S) (NEB). Input DNA was used as a control and for normalization. Modifications to the Illumina protocol were: 1- 30 ng of ChIP products were used as template, 2- paired-end adapters were ligated to end-repaired and A-tailed DNA using T4 DNA ligase (NEB) for 2 hours at 16°C, and 2- Phusion polymerase in GC buffer (Finzyme) was used instead of Phusion polymerase in HF buffer. DNA products were purified with QIAquick spin columns (Qiagen). Concentration, size distribution (400-500bp), and purity of libraries were assessed on  DNA1000 Bioanalyzer chip (Agilent). Genome Analyzer II (Illumina) was used to perform 2x36 cycles of paired end sequencing.
attributes.tag: GEO Accession
attributes.value: GSM905447