title: Illumina HiSeq 2000 sequencing; H3K4me3 and H3K27me3 chromatin states in the Arabidopsis shoot phloem companion cells from INTACT reporter lines
design: H3K4me3 and H3K27me3 chromatin states in the Arabidopsis shoot phloem companion cells from INTACT reporter lines
library_construction_protocol: Plants of the shoot-phloem-companion-cell-tagged INTACT reporter line (Transgenic SUC2::redNTF and UBQ10::BirA in Col-0 background) were cultivated in growth chambers, in short-day (SD) conditions (8 h light/16 h dark) for 21 days and then shifted to long-day (LD) conditions (16 h light/8 h dark) to induce flowering.  The growth-chamber condition was at 23°C with 65% relative humidity, and with the light at a fluence rate of 125 to 175 μmol m−2 s−1. ChIP DNA was prepared from INTACT purified nuclei from the meristem, as described in Deal, R. B. & Henikoff, S.  (2010) Nat. Protoc.  6, 56-68; and You et al. (2017): DOI: 10.1038/ncomms15120. Libraries were prepared from approximately 50 pg of ChIP DNA using the ThruPlex-FD Prep Kit (Rubicon Genomics)
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: H3
attributes.tag: Experimental Factor: sampling time point
attributes.value: 0