title: buffalo_RNA-seq_mammary gland tissue
design: The cDNA library for each sample was prepared by IlluminaTruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturers instructions. Briefly, 5 g of RNA for each sample was used for RNA-seq library preparation. The poly (A) mRNAs were isolated from total RNA using the poly-T oligo-attached magnetic beads. The cDNA synthesis was performed using the SuperScript II, DNA Polymerase I and RNase H treatment, and then chemically fragmented to ~200 nt fragments and enriched with PCR to create the final cDNA libraries.