title: Nanopore WGS of  Labeo rohita: adult male blood
design: For each Nanopore sequencing run, 2 to 2.5 g of genomic DNA was used in the library preparation with Nanopore Genomic DNA Ligation Sequencing Kit SQK-LSK 109 (Oxford Nanopore Technologies, Oxford, UK). The final library (about 700 to 750 ng) was loaded on a Nanopore Flow Cell R9.4.1 (Oxford Nanopore Technologies, Oxford, UK) and sequenced on GridION sequencer (Oxford Nanopore Technologies, Oxford, UK) for 48 hours. A total of 10 flow cell runs were conducted for the genome assembly.