title: NGS of Tsomgo lake, Sikkim India
design: The paired-end sequencing library was prepared using Truseq Nano DNA Library Prep kit. The library preparation process was initiated with 200ng of gDNA. The gDNA was mechanically sheared into desired fragments by Covaris instrument followed by continuous step of end-repair where an A is added to the 3 ends making the DNA fragments ready for adapter ligation. Following this step, Illumina specific adapters are ligated to both ends of the DNA fragments. These adapters contain sequences essential for binding dual-barcoded libraries to a flow cell for sequencing, allowing for PCR amplification of adapter-ligated fragments, and binding standard Illumina sequencing primers. To ensure maximum yields from limited amounts of starting material, a high-fidelity amplification step was performed using HiFi PCR Master Mix.