title: borage4-seeds-transcriptome
design: Total RNA was extracted using the TianGen RNAprep Pure Plant Kit (Tiangen Biotech Co. Ltd, Beijing, China) following the manufacturer s protocol. All RNA was treated with RNase-free DNase I provided by the kit. The integrity of RNA was evaluated by running the samples on a 1.2 % (w/v) agarose gel with Goldview. The quantity of RNA was determined using a NanoDrop ND-2000 spectrophotometer. The concentration and purity of RNA were measured using the Agilent 2100 Bioanalyzer (RNA Nano Chip, Agilent).