title: DNA-Seq of Prokaryotes: Alkaline Hot springs
design: DNA extraction from environmental samples using phenol-chloroform  protocol, PCR amplification of 16S rRNA gene variable region (V4 – V7) and library construction following Illumina sequencing protocol. Barcode sequence, name, linker: ATCGATGC; ITS1F.Bt1; CTTGGTCATTTAGAGGAAGTAA ATCGGCTA; ITS1F.Bt7; CTTGGTCATTTAGAGGAAGTAA ATCGCGAT; ITS1F.Bt4; CTTGGTCATTTAGAGGAAGTAA ATCGGCAT; ITS1F.Bt6; CTTGGTCATTTAGAGGAAGTAA  