title: R2 Cathode
design: Community DNAs were subjected to bar-coded amplicon library preparation and pyrosequencing using the 454 Genome Sequencer FLX Titanium System at The Pennsylvania State University Genomics Core Facility. An amplicon library was prepared for each sample by PCR using universal bacterial primers (27F-907R) complementary to regions V1-V5 of the 16S rRNA gene