title: Virome of Lake Pavin : 80 meters
design: water sample was filtered on 0.22 m, and virus-like particles were concentrated by tangential ultrafiltration and PEG precipitation. Concentrates were treated with DNaseI , encapsidated DNA was freed via a thermal shock, purified with a QIAamp DNA mini kit (Qiagen), and randomly amplified with the phi29 polymerase using random hexamer primers (Genomiphi Kit, GE Healthcare)