title: 16S rRNA of mus musculus intestine: FMT transplanted ex-germ-free adult
design: DNA from up to 250 mg of intestinal contents was immediately extracted using the MoBio/QIAamp PowerFecal kit according to the manufacturers instructions or kept at 80 C until further processing. DNA was quantified using a NanoDrop, and samples were diluted to 2.5 ng l1 in MQ water. Sequencing libraries were generated by amplifying the V4 region of the 16S rRNA gene using barcoded primers. The whole PCR reaction was run on 1.0% agarose/TBE gel and bands were extracted using QIAGEN QIaquick gel extraction kit.