title: GSM4704162: PFC_Zbtb16_WT1; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was extracted from prefrontal cortex (PFC) of male mice at 7 weeks with the AllPrep DNA/RNA Mini Kit (#80204, Qiagen, Hilden, Germany) according to the manufacturer's instruction. RNA integrity number (RIN) of total RNA was quantified by Agilent 2100 Bioanalyzer using Agilent RNA 6000 Pico Kit (#5067-1513, Agilent, Santa Clara, CA). RNA-seq was performed as a service by Macrogen Japan Corp. (Kyoto, Japan). Briefly, total RNA with RIN values of ≥8.1 were used for RNA-seq library preparation. mRNA was purified from 500 ng total RNA, and subjected to cDNA library making (fragmentation, first and second strand syntheses, adenylation, ligation and amplification) by TruSeq Stranded mRNA Library Prep (#20020594, Illumina, San Diego, CA) according to the manufacturer's instruction. cDNA library quality was quantified by 2100 Bioanalyzer using Agilent High Sensitivity DNA Kit (#5067-4626, Agilent, Santa Clara, CA). Library was sequenced as 101 bp paired-end on Illumina NovaSeq6000.
attributes.tag: GEO Accession
attributes.value: GSM4704162