title: Molecular biological and stable isotope views of how combination type affects nitrogen removal pathway in hybrid constructed wetlands treating raw sewage
design: Illumina high-throughput sequencing was performed on substrate samples collected from the HCW with the highest TN removal to discern the nitrogen transforming community. Amplicon libraries were constructed for the high-throughput sequencing using bacterial primers 338F 5'-ACTCCTACGGGAGGCAGCA-3' and 806 R 5'-GGACTACHVGGGTWTCTAAT-3' targeting V3-V4 regions.