title: BS-seq of Arabidopsis thaliana: inflorescence
design: DNA of inflorescences was purified using a MagJET plant genomic DNA Kit (Thermo Fisher Scientific) and eluted in 80 L of elution buffer. The DNA was fragmented into 200-300--bp fragments using Covaris S220. After adaptor ligation, bisulfite treatment was performed using an EZ DNA Methylation Gold Kit (Zymo Research). PCR amplification of the library molecules was performed using ZymoTaq DNA polymerase (Zymo Research). Total RNA from the inflorescences was extracted with a RNeasy Plant Mini kit (Qiagen). For RNA-seq analysis, the total RNA from inflorescence was treated with a Poly(A) mRNA Magnetic Isolation Module Kit (NEB #E7490). Libraries were prepared from the resulting mRNA with a NEBNext Ultra Kit (NEB #E7530). The libraries were constructed according to a standard protocol (Illumina).