title: CD86 transmembrane domain deep mutational scanning library introduced into HeLa cells
design: Deep mutational Scanning Library targeting CD86 residues 244-270 cloned into retroviral expression vector with GFP reporter. Post transduction, GFP positive HeLa cells were sorted, mRNA isolated and amplicons generated to sequence variants. Each transduction was performed 3 times. Each amplicon was generated using three different index pairs.