alias: E-MTAB-9222:72872_s
title: NextSeq 550 sequencing; Genotyping-by-sequencing (GBS) of wild and cultivated Lens accessions and the domestication history of lentils
design: Genotyping-by-sequencing (GBS) of wild and cultivated Lens accessions and the domestication history of lentils
library_construction_protocol: Seeds were ordered from germplasm banks: ICARDA (Lebanon), USDA-GRIN (USA), IPK (Germany) and Nordgen (Sweden) All extractions were made with the DNeasy® Plant Mini Kit (Qiagen) and DNA was quantified with a Qubit™ dsDNA HS assay on a Qubit™ 2.0 Fluorometer. DNA integrity for GBS was checked by running all extractions on a 1% agarose gel. Two 96-well plates were prepared with each well containing 300ng of DNA.  Libraries, including indexing, were prepared using a combination of PstI and ApeKI restriction enzymes.
identifiers.namespace: Centre for Biomedical Research (CBMR), University of Algarve
identifiers.id: E-MTAB-9222:72872_s
attributes.tag: Experimental Factor: country of origin
attributes.value: Syria
attributes.tag: Experimental Factor: strain
attributes.value: Lens culinaris subsp. orientalis