alias: GSM4932364
title: GSM4932364: HEK293T_dCas9_dMSK1_non-targeting_gRNAs_re1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen, #74136) following manufacturer's instructions RNA-seq library construction using the TruSeq Stranded Total RNA Gold (Illumina, RS-122-2303). The qualities of RNA-seq libraries were verified using the Tape Station D1000 assay (Tape Station 2200, Agilent Technologies) and the quantities of RNA-seq libraries were checked again using real time PCR (QuantStudio 6 Flex Real time PCR System, Applied Biosystem). Libraries were normalized and pooled and then 75-bp paired-end reads were sequenced on the Hiseq 3000 platform (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM4932364
attributes.tag: GEO Accession
attributes.value: GSM4932364