alias: Small RNA sequencing of Penicillium chrysogenum P2niaD18
title: Small RNA sequencing of Penicillium chrysogenum strain P2niaD18
design: For pooled total RNA sample, equal amounts of total RNA were isolated by guanidinium-thiocyanate-phenol-chloroform extraction from six submerged cultures in MM and CCM, representing three different time points (72, 96, and 120 h). Furthermore, six surface cultures, grown on M322 and Oatmeal were harvested after 48, 72, and 96 h for preparing samples. The pooled total RNA sample was separated by a 10% TBE-urea denaturing polyacrylamide gel electrophoresis (PAGE) and sRNAs ranging between 18 and 50 nt were used for library preparation using the Illumina TruSeq Small RNA Sample Preparation Kit. After reverse transcription and amplification, cDNA products were sequenced on an Illumina HiSeq 2000 platform.
identifiers.namespace: Ruhr-Universitaet Bochum
identifiers.id: Small RNA sequencing of Penicillium chrysogenum P2niaD18