alias: GSM3638642_2
title: GSM3638642: WT Male 5 days rep2; Drosophila melanogaster; Bisulfite-Seq
library_construction_protocol: 500-1,000 embryos, ~50 larvae, ~50 pupa, ~50 ovaries, and ~50 adult flies were used for RNA isolation in each stage.  Total RNA was isolated with TRIzol reagent and Direct-zol RNA MiniPrep kit. Polyadenylated RNA was separated from total RNA using Oligo dT Magnetic Beads (Vazyme). 100ng-1?g of polyadenylated RNA was converted using the EZ RNA methylation kit (Zymo Research) with modified high-stringency conversion conditions. The quantity of the converted RNA was determined by Qubit. 20ng converted RNA was fragmented into 150~200 nt fragments by incubation at 94 °C for 8min in fragmentation buffer (NEBNext Ultra II Directional RNA Library Prep Kit, NEB). The fragmented RNA was then used for library construction, following the manufacturer's protocol (NEBNext Ultra II Directional RNA Library Prep Kit, NEB).
identifiers.namespace: GEO
identifiers.id: GSM3638642_2
attributes.tag: GEO Accession
attributes.value: GSM3638642