alias: LDExo2012
title: Leishmania donovani exosome transcriptome sequencing
design: Exosomes were purified from supernatant of day 5 promastigotes incubated at lowered pH and elevated temp (early amastigotes). RNA was purified with phenol/chloroform and DNase treated. Custom construction method optimized for short sequences: CIP, TAP, PNK treatments to generate uniform 5'P, ligation of short DNA adapter to 3' end followed by ligation of RNA adapter to 5' end, RT, ampl, ligation of Illumina proprietary adapters, sequencing.
identifiers.namespace: University of British Columbia
identifiers.id: LDExo2012