alias: Allorhogas_belonocnema_CNIN3391
title: UCE target enrichment of Allorhogas_belonocnema_CNIN3391: whole organism
design: Genomic DNA was extracted using the Spin Column Genomic DNA Minipreps Kit (Bio Basic). Digestion of protein components was carried out overnight in 300 L of digestion solution and 20 L of proteinase K, and the supernatant was subsequently processed following the manufacturers protocol. All DNA extractions were quantified using a Qubit 2.0 (Life Technologies) and for each sample up to 50 ng of extracted DNA was fragmented to a mean fragment size of 400-600 bp using a BioRuptor Pico sonication device. Libraries were generated using Kapa Hyper Prep kits (Kapa Biosystems Inc., Wilmington, MA) and custom, TruSeq-style dual-indexing barcodes. For enrichment, we pooled libraries at equimolar concentrations and used the first version of the Mybaits RNA bait library developed for Hymenoptera and synthesized by Arbor Biosciences (formerly MYcroarray, Ann Arbor, MI) following the standard enrichment protocol for UCEs (available from ultraconserved.org). The enriched pool were sent for sequencing to the Georgia Genomics Facility, University of Georgia, Athens, USA, on an Illumina NextSeq v2 300 cycle kit.
identifiers.namespace: SUB7864375
identifiers.id: Allorhogas_belonocnema_CNIN3391