alias: GSM1843530
title: GSM1843530: Wild-type Villus H3K27me3 ChIP-Seq rep 1; Mus musculus; ChIP-Seq
library_construction_protocol: Epithelium from proximal 1/3 of mouse intestine was isolated by incubation with 5mM EDTA in PBS for 45 minutes at 4°C. Cells were snap-frozen in liquid nitrogen and pooled for subsequent ChIP analysis. Whole cell lysates were sonicated and incubated with 5 ug H3K27me3 antibody overnight (07-449, Millipore) for immunoprecipitation. The ChIPseq DNA Sample Prep Kit (Illumina) was used to prepare libraries, and 50 bp single-end reads were sequenced on an Illumina Hi-Seq 2000 instrument.
identifiers.namespace: GEO
identifiers.id: GSM1843530
attributes.tag: GEO Accession
attributes.value: GSM1843530