alias: GSM1563526
title: GSM1563526: ISC at P0 (RNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: Qiagen ALLPREP DNA/RNA Mini kit for RNA extraction. 10ng of total RNA was used for cDNA synthesis.  First, a universal primer I with oligoT was used to get the first strand cDNA.  The free universal primer I was removed by exonulease I (Takara Bio Inc). PolyA tailing reaction was performed by Terminal Transferase (TdT) (Invitrogen). After that, a universal primer II was used to synthesize the second strand cDNA. PCR amplification was performed to enrich the dscDNA. The amplified cDNA was sized selected by running a 1% agarose gel and the fragment from 500bp-3kb was cut and purified. The gel purified fragment was sheared to 300-500bp. The library preparation followed the standard DNA preparation protocol from Illumina. The Illumina library was quantified by picogreen assay (Invitrogen) and bioanalyzer (Agilent).  The quantified library was sequenced on Illumina Hiseq 2000 platform according to the manufacturer’s protocol.
identifiers.namespace: GEO
identifiers.id: GSM1563526
attributes.tag: GEO Accession
attributes.value: GSM1563526