alias: GSM4152222
title: GSM4152222: AL1Heavy; aquifer metagenome; RNA-Seq
library_construction_protocol: RNA was extracted using a bead-beating phenol-chloroform protocol modified from (Schmitt et al. 1990), shaken with a Tissuelyzer II (QIAgen) instead of a beadbeater and precipitated using 1 vol isopropanol and 0.5 vol 7.5 M ammonium acetate. After isopycnic centrifugation, RNA was converted to double stranded cDNA using the SuperScript IV First-Strand Synthesis System and SuperScript Double-Stranded cDNA Synthesis Kit (Thermofisher). Library preparation was performed using the Nextera XT kit (Illumina) according to manufacturer protocols.
identifiers.namespace: GEO
identifiers.id: GSM4152222
attributes.tag: GEO Accession
attributes.value: GSM4152222