alias: delta-dcl2/dcl1
title: Small RNA sequencing of the Dicer-deficient Penicillium chrysogenum strain delta-dcl2/dcl1
design: Total RNA sample was isolated by guanidinium-thiocyanate-phenol-chloroform extraction from from a 72 h submerged CCM culture (27°C, 180 r.p.m.) The total RNA sample was separated by a 10% TBE-urea denaturing polyacrylamide gel electrophoresis (PAGE) and sRNAs ranging between 18 and 50 nt were used for library preparation using the Illumina TruSeq Small RNA Sample Preparation Kit. After reverse transcription and amplification, cDNA products were sequenced on an Illumina HiSeq 2000 platform.
identifiers.namespace: Ruhr-Universitaet Bochum
identifiers.id: delta-dcl2/dcl1