alias: GSM1564658
title: GSM1564658: SI_8041-4-5.7-mdv-polya; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the Qiagen AllPrep Kit (cat. no. 80404) Details of the capture RNA-seq and poly(A) RNA-seq library preparation protocols are provided as Supplementary Data. Briefly, for capture libraries we start with 1-3µg of total RNA, and proceed through first-strand synthesis, second strand synthesis, end repair, A-tailing, adapter ligation, size selection on 3% agarose gel, uridine digestion, hybridization to capture probes, washing, and a final PCR step. For poly(A) libraries the protocol is the same, but the last capture and PCR step is omitted and replaced with oligo dT bead selection on input RNA. The stranded capture and poly(A) libraries were sequenced on an Illumina HiSeq 2500 using V3 chemistry.
identifiers.namespace: GEO
identifiers.id: GSM1564658
attributes.tag: GEO Accession
attributes.value: GSM1564658