alias: besansky-parentals-only
title: Multiplexed shotgun genotyping of parental Anopheles merus and Anopheles coluzzii: Sample AnMSG_FILE1
design: We constructed multiplexed shotgun genotyping libraries as described in Andolfatto 2011, and on this lane pooled individual MSG libraries of 384 backcross progeny, 192 parental Anopheles coluzzii, and 192 parental Anopheles merus. For multiplexing, each individual mosquito was assigned a unique index identifying the multiplexed library, which is needed for demultiplexing, and one of 48 barcodes identifying individuals. Backcross progeny from this lane are in sequencing set 1. The library names in this sequencing lane and their indices are as follows MaliparentFC1_1 ATCACG, MaliparentFC1_2 CGATGT, MaliparentFC1_3 TTAGGC, MaliparentFC1_4 TGACCA, MAFparentFC1_5 ACAGTG, MAFparentFC1_6 GCCAAT, MAFparentFC1_7 CAGATC, MAFparentFC1_8 ACTTGA, BCtolerantFC1_9 GATCAG, BCtolerantFC1_10 TAGCTT, BCtolerantFC1_11 GGCTAC, BCtolerantFC1_12 CTTGTA, BCsusceptibleFC1_13 AGTCAA, BCsusceptibleFC1_14 AGTTCC, BCsusceptibleFC1_15 ATGTCA, BCsusceptibleFC1_16 CCGTCC.
identifiers.namespace: University of Notre Dame
identifiers.id: besansky-parentals-only