alias: day30
title: RNA-seq of day 30 human iPSCs-derived mammary-like organoids
design: After the total RNA was qualified, eukaryotic mRNAs were enriched with magnetic beads linked with Oligo(dT).The extracted mRNA was fragmented randomly into short fragments by Fragmentation Buffer. Using the fragmented mRNA as the template, a strand of cDNA was synthesized using Random Hexamers. Then, Buffer, dNTPs, RNaseH and DNA Polymerase I were added for the two-strand cDNA synthesis. The AMPureXP purification of double-stranded products in Beads was carried out by using T4DNA polymerase and KlenowDNA polymerase activity to repair the sticky ends of DNA into flat ends. The 3 'ends were added with base A and fittings. The AMPureXP Beads were selected for fragments, and finally PCR amplification was carried out to obtain the final sequencing library.
identifiers.namespace: SUB7900439
identifiers.id: day30