alias: GSM4717168
title: GSM4717168: HEK293T-25ng-NAIN3-rep1; Homo sapiens; OTHER
library_construction_protocol: For smartSHAPE and RNA-Seq, RNAs were isolated with Trizol reagents. For smartSHAPE, cells were treated with NAI-N3 or DMSO and RNAs were isolated with Trizol followed by rRNA removal. RNAs were reverse transcribed with random primers, and RNase I digestion was performed at 37 °C for 30 min. Enrichment was performed using MyOne C1 beads. After elution, cDNAs were ligated with biotinylated 3' adaptor using CircLigase and immobilized with MyOne C1 beads. Second strand synthesis and 5'ligation was performed on the beads. smartSHAPE libraries were generated by amplification of ligation products. RNA seq libraries of Reg1 KO and WT iBMDMs were generated with KAPA RNA Hyper Prep kit. uvCLAP libraries were generated as published protocol (PMID: 29559621).
identifiers.namespace: GEO
identifiers.id: GSM4717168
attributes.tag: GEO Accession
attributes.value: GSM4717168