alias: GSM4766505
title: GSM4766505: Single cell TCR sequencing of CD4+ Tcells from the intestinal epithelium 5; Mus musculus; OTHER
library_construction_protocol: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol) and frozen in -80°C prior to RT-PCR. RNA and RT-PCRs for TCRa and TCRb were prepared as previously described (Dash et al., 2011). PCR products for TCRa and TCRb were either subjected to Sanger sequencing or multiplexed with barcodes and submitted to MiSeq sequencing (Han et al., 2014) using True Seq Nano kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM4766505
attributes.tag: GEO Accession
attributes.value: GSM4766505