alias: E-MTAB-5493:M409 Day21_s
title: Illumina HiSeq 2500 sequencing; Time-series RNA-seq experiment performed on nine melanoma cell lines treated with BRAF inhibitor vemurafenib.
design: Time-series RNA-seq experiment performed on nine melanoma cell lines treated with BRAF inhibitor vemurafenib.
library_construction_protocol: Cells are cultured in RPMI1640 medium. Medium was drug is replenished every 2days. The dose of vemurafenib for each cell line is the 2 times of the IC50 (half growth inhibition) value of each cell line. The dose of TNF is 1000IU/ml. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: Division of Chemistry and Chemical Engineering, Caltech
identifiers.id: E-MTAB-5493:M409 Day21_s
attributes.tag: Experimental Factor: compound
attributes.value: vemurafenib
attributes.tag: Experimental Factor: time
attributes.value: 21
attributes.tag: Experimental Factor: cell line
attributes.value: M409