alias: library_bacteria
title: Bacterial diversity analysis: eggs sample
design: Gemonic DNAs were extracted from the egg samples. Primers 515F and 806R were used to amplify the V4 region of the 16S rDNA. A bar code sequence was added at the 5-end to discriminate each sample. PCR was conducted in triplicate, and the products were pooled for each subsample. A composite DNA sample for sequencing was created by combining equimolar ratios of amplification products from each sample.
identifiers.namespace: SUB6512857
identifiers.id: library_bacteria