alias: GSM4873056
title: GSM4873056: IP_H3; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: anti-GFP and anti-H3 ChIP were performed as follow: crosslinked dissected cotyledons were frozen in liquid nitrogen and ground in Eppendorf tubes using a Tissue Lyser (Qiagen) for 90 seconds at 30 Hz. Chromatin was extracted and resuspended in 100 µL Nuclei Lysis Buffer, the samples were flash frozen in liquid nitrogen to help breaking the nuclei. Chromatin was sheared using a S220 Focused-ultrasonicator (Covaris) for 17 min at 105 W of power to obtain fragments between 75 and 300 bp. ChIP-Seq TruSeq kit
identifiers.namespace: GEO
identifiers.id: GSM4873056
attributes.tag: GEO Accession
attributes.value: GSM4873056