alias: 13
title: SO_5203_373
design: About 200 nanograms of genomic DNA from each sample was made up to 50 ul with Low TE and sonicated for ~210 sec on covaris to fragment DNA ranging between ~200 to 400 bp. DNA was subjected to enzymatic reactions that repair frayed ends, phosphorylate the fragments, add a single nucleotide A overhang and ligate adaptors. Adaptors ligated fragments was enriched using 10 cycles PCR amplification. The prepared library was then kept for hybridization. These were isolated by magnetic capture using Magnetic Streptavidin coated beads. PCR product was cleaned up using HighPrep PCR clean up system.
identifiers.namespace: SUB7654083
identifiers.id: 13