alias: GSM3722699
title: GSM3722699: K562-dCas9-KRAB_NC_sgNC-1_2; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted from cells using Trizol Reagent (Thermo), and were subjected to enrichment of polyA+ RNA using the NEBNext Poly(A) kit (NEB). Strand-specific polyA+ RNA-Seq was performed using the LM-Seq method, as described previously. Briefly, PolyA+ RNA was fragmented at 85°C in SmartScribe first-strand buffer (CloneTech) supplemented with a 3' sequencing adapter containing a random hexamer sequence.  The fragmented RNA were subjected to first strand cDNA synthesis, followed by RNase A/H treatment.  The remaining single-stranded DNA were ligated to a 5' sequencing adapter.  The libraries were then amplified by PCR using Illumina TruSeq-compatible primers and purified using SPRI purification beads.
identifiers.namespace: GEO
identifiers.id: GSM3722699
attributes.tag: GEO Accession
attributes.value: GSM3722699