alias: HA_K2_16S-V4
title: 16S-V4 amplicon sequencing of beer metagenome: King's ale K2
design: Metagenomic profiling of extracted DNA was conducted by MrDNA services, Texas, USA ( http://www.mrdnalab.com/sequencing-service.html) using Ion Torrent S5 SL PGM and primers 515F-806R for the V4 region of the bacterial 16S ribosomal gene. Amplicons were generated in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 30 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequence data were processed using a proprietary  analysis pipeline.  In summary, sequences were depleted of barcodes and primers, then sequences <150bp removed, sequences with ambiguous base calls and with homopolymer runs exceeding 6bp were also removed.
identifiers.namespace: SUB8385014
identifiers.id: HA_K2_16S-V4