alias: sHL3-nifH
title: BSC: nitrogen fixation bacteria
design: The resulted PCR products were extracted from a 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantiFluor-ST (Promega, USA) according to the manufacturers protocol. Purified amplicons were pooled in equimolar and paired-end sequenced (2  312) on an Illumina MiSeq platform (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).
identifiers.namespace: SUB7847927
identifiers.id: sHL3-nifH