alias: Amel_egg
title: The dynamic DNA methylation cycle from egg to sperm in the honey bee Apis mellifera
design: Fragments were end repaired (Illumina) as recommended by the manufacturer. Repaired fragments were ligated with methylated sequencing adaptors using a paired end adaptor oligo kit and oligo mix (Illumina). Ligated fragments were selected by gel electrophoresis and fragments of 360 bp size extracted using a QIAquick gel extraction kit (Qiagen); size-selected fragments were bisulfite treated using an EZ-DNA methylation kit (Zymo Research) and enriched using a MethylMiner methylated DNA enrichment kit (Invitrogen); Libraries were amplified using T4 polymerase (Enzymatics) and sequenced on an Illumina HiSeq platform.
identifiers.namespace: SUB5839902
identifiers.id: Amel_egg