alias: 16S_SW_9A
title: 16S rRNA gene amplicon sequencing of seawater DNA: Sample SW09
design: Amplification of the 16S rRNA gene was performed as detailed in Bell et al (5) using primers E786 and U926 designed to target the hypervariable V5 region, with the difference that we used 26 cycles of amplification instead of 30 cycles. After verification of successful amplification, products were run on a 2% agarose gel and excised for gel purification using the Qiagen Qiaquick Gel Extraction kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. DNA was eluted in 50microl Ambion nuclease-free water (Life Technologies) and quantified using the Picogreen method according to the manufacturer's instructions. Samples were diluted to 5X109 moleculesmicrol-1, and 10microl of each was used for emulsion PCR and Ion Torrent Personal Genome Machine (PGM) sequencing on a 314 chip as described in Sanschagrin and Yergeau (44). 
identifiers.namespace: NRCC-Biotechnology Research Institute
identifiers.id: 16S_SW_9A