alias: GSM1845111
title: GSM1845111: piwi_sh_CAPseq; Drosophila melanogaster; OTHER
library_construction_protocol: Cap-seq was performed according to Nachaev et al, Science, 2010 and Gu et al, Cell, 2013 Purified Capped RNA fragments were ligated with 3' and 5' linkers. 1st strand cDNA synthesis was performed using SuperScript III (Invitrogen) and the library was amplified using the Kapa HiFi Library Amp real time Kit (Peqlab) with Illumina TruSeq compatible primers.
identifiers.namespace: GEO
identifiers.id: GSM1845111
attributes.tag: GEO Accession
attributes.value: GSM1845111