alias: E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep2_s
title: NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome
design: RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome
library_construction_protocol: Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).
identifiers.namespace: LUMC
identifiers.id: E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep2_s
identifiers.namespace: Leiden University Medical Center
identifiers.id: E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep2_s
attributes.tag: Experimental Factor: dose
attributes.value: 10
attributes.tag: Experimental Factor: compound
attributes.value: sodium crotonate