alias: GSM4103366
title: GSM4103366: F-KO1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from FACS-sorted C-LTMRs (GFP+) using RNeasy Micro Kit (Quiagen) following the manufactures protocol.RNA quality was assessed using the RNA Agilent 2100 Bioanalyzer system (RNA Pico chips). Full length cDNA were generated from 500 pg of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe, Saint Germain en Laye, France) according to manufacturer's instructions with 13 cycles of PCR for cDNA amplification by Seq-Amp polymerase. Six hundreds pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification. Following purification with Agencourt AMPure XP beads (Beckman-Coulter, Villepinte, France), the size and concentration of libraries were assessed by capillary electrophoreris.
identifiers.namespace: GEO
identifiers.id: GSM4103366
attributes.tag: GEO Accession
attributes.value: GSM4103366