alias: GSM3581497
title: GSM3581497: Wood duck Replicate 1; Aix sponsa; RNA-Seq
library_construction_protocol: Trigeminal ganglia were dissected from embryos, flash-frozen and stored at -80°C until RNA isolation. Total RNA was isolated from the stored trigeminal ganglia using the TRIzol reagent (ThermoFisher, Waltham, MA) according to manufacturer's instructions. RNA quality and integrity was confirmed by running on Agilent Bioanalyzer, with RINs in the range of 7.7-8.6. mRNA was purified from ~500 ng total RNA with oligo-dT beads. Strand-specific sequencing libraries were prepared using the KAPA mRNA Hyper Prep kit (Roche Sequencing, Pleasanton, CA).
identifiers.namespace: GEO
identifiers.id: GSM3581497
attributes.tag: GEO Accession
attributes.value: GSM3581497