alias: E-MTAB-7963:BA1_E11.5_Rep1_p
title: Illumina HiSeq 2500 paired end sequencing; RNA-seq analysis of the first branchial arch of the mouse embryos at E10.5 and E11.5
design: RNA-seq analysis of the first branchial arch of the mouse embryos at E10.5 and E11.5
library_construction_protocol: CD1 mice were time-mated to obtain branchial arches. Branchial arches were dissected and snap frozen in dry ice.  RNA was extracted using Trizol (Invitrogen, 15596-018), pooling 3-5 pairs of arches for each sample. RNA-seq was performed in duplicates.  Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina)
identifiers.namespace: UNIVERSITY OF MANCHESTER
identifiers.id: E-MTAB-7963:BA1_E11.5_Rep1_p
attributes.tag: Experimental Factor: age
attributes.value: embryonic day 11.5