alias: qiime_experiment_1890:1000299:70
title: qiime_experiment_1890:1000299:70
design: The indoor microbiome is a complex system that is thought to depend on dispersal from the outdoor biome and the occupants' microbiome combined with selective pressures imposed by the occupants' behaviors and the building itself. We set out to determine the pattern of fungal diversity and composition in indoor air on a local scale and to identify processes behind that pattern. We surveyed airborne fungal assemblages within 1-month time periods at two seasons, with high replication, indoors and outdoors, within and across standardized residences at a university housing facility. Fungal assemblages indoors were diverse and strongly determined by dispersal from outdoors, and no fungal taxa were found as indicators of indoor air. There was a seasonal effect on the fungi found in both indoor and outdoor air, and quantitatively more fungal biomass was detected outdoors than indoors. A strong signal of isolation by distance existed in both outdoor and indoor airborne fungal assemblages, despite the small geographic scale in which this study was undertaken (<500???m). Moreover, room and occupant behavior had no detectable effect on the fungi found in indoor air. These results show that at the local level, outdoor air fungi dominate the patterning of indoor air. More broadly, they provide additional support for the growing evidence that dispersal limitation, even on small geographic scales, is a key process in structuring the often-observed distance-decay biogeographic pattern in microbial communities.
library_construction_protocol: Samples were PCR-amplified in triplicate and pooled before cleaning using AMPure magnetic beads. Amplicon samples were then individually quantified using the Qubit flourometer and pooled to an equimolar concentration.
identifiers.namespace: CCME-COLORADO
identifiers.id: qiime_experiment_1890:1000299:70
attributes.tag: experiment_title
attributes.value: Adams_fungi_house_dust
attributes.tag: key_seq
attributes.value: TCAG
attributes.tag: sample_center
attributes.value: UCBerkeley_Bruns
attributes.tag: sequencing_meth
attributes.value: pyrosequencing
attributes.tag: run_date
attributes.value: 5/14/12
attributes.tag: experiment_center
attributes.value: UCBerkeley_Bruns
attributes.tag: pcr_primers
attributes.value: FWD:TGGTCATTTAGAGGAAGTAA; REV:GCTGCGTTCTTCATCGATGC
attributes.tag: run_center
attributes.value: UCBerkeley_Bruns
attributes.tag: num_sequences
attributes.value: None
attributes.tag: platform
attributes.value: Titanium
attributes.tag: library_construction_protocol
attributes.value: Samples were PCR-amplified in triplicate and pooled before cleaning using AMPure magnetic beads. Amplicon samples were then individually quantified using the Qubit flourometer and pooled to an equimolar concentration.
attributes.tag: experiment_design_description
attributes.value: The indoor microbiome is a complex system that is thought to depend on dispersal from the outdoor biome and the occupants' microbiome combined with selective pressures imposed by the occupants' behaviors and the building itself. We set out to determine the pattern of fungal diversity and composition in indoor air on a local scale and to identify processes behind that pattern. We surveyed airborne fungal assemblages within 1-month time periods at two seasons, with high replication, indoors and outdoors, within and across standardized residences at a university housing facility. Fungal assemblages indoors were diverse and strongly determined by dispersal from outdoors, and no fungal taxa were found as indicators of indoor air. There was a seasonal effect on the fungi found in both indoor and outdoor air, and quantitatively more fungal biomass was detected outdoors than indoors. A strong signal of isolation by distance existed in both outdoor and indoor airborne fungal assemblages, despite the small geographic scale in which this study was undertaken (<500???m). Moreover, room and occupant behavior had no detectable effect on the fungi found in indoor air. These results show that at the local level, outdoor air fungi dominate the patterning of indoor air. More broadly, they provide additional support for the growing evidence that dispersal limitation, even on small geographic scales, is a key process in structuring the often-observed distance-decay biogeographic pattern in microbial communities.
attributes.tag: target_gene
attributes.value: ITS1
attributes.tag: row_number
attributes.value: 70
attributes.tag: study_center
attributes.value: UCBerkeley_Bruns