alias: mmu_rna_seq_rep_2_TRANSCRIPTOMIC
title: RNA-Seq of Mus musculus NIH/3T3: Replicate 2: Sample Mus musculus NIH/3T3
design: "Cells were cultured in RPMI medium (Sigma) at 37 degrees C and 5% CO2 and collected at approximately 70% confluency after trypsinization. Directional RNA-seq libraries were prepared according to the Illumina-provided protocol. Poly(A)-containing RNA was isolated from the cells with the ""Dynabeads mRNA DIRECT Kit"" (Ambion) and fragmented by alkaline hydrolysis to fragment sizes of 150-300 nt."
identifiers.namespace: Biozentrum, University of Basel
identifiers.id: mmu_rna_seq_rep_2_TRANSCRIPTOMIC
attributes.tag: alignment_software
attributes.value: segemehl 0.1.7-407