alias: MG_ICE_22
title: Shotgun metagenomic sequencing of sea ice DNA: Sample ICE22
design: Each DNA library was prepared for sequencing using the Ion Xpress Plus Fragment Library Kit (Life Technologies) with the Ion Xpress Barcode Adapters 1-16 (Life Technologies), using the Ion Shear Plus Reagents and a Pippin Prep instrument (SAGE Science, Beverly, MA) for size-selection.  Barcoded libraries were pooled in an equimolar ratio three by three. A total of 3.50 x 107 molecules were used in an emulsion PCR using the Ion Xpress 200 Template Kit (Life Technologies) as described in Sanschagrin and Yergeau (44). The sequencing of the pooled libraries was performed using the PGM system with the Ion Sequencing 200 kit and 316 chips.
identifiers.namespace: NRCC-Biotechnology Research Institute
identifiers.id: MG_ICE_22