alias: GSM1563223
title: GSM1563223: Control_H3K4me3_ChIPSeq_R1; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates from testis cells were sonicated, clarified and DNA protein complexes were isolated with anti H3K4me3 antibodies. Immunoprecipitated DNA was purified from proteins.The DNA concentration was measured with Quantus Fluoremeter (E6150, Promega), using Quantiflor dsDNA system, (E2670, Promega). The libraries were prepared from 50 ng of ChiP and Input material using NEBNext Ultra DNA library Prep Kit for Illumina (NEB #E7370S). Library selection was done using excision fragments from 2% agarose gel including GelGreen stain (Gentaur, 41005), 180-220 bp fragments were excised using Dark reader transilluminator (Clare Chemical research, Dr-46B). The Illumina Hiseq2000 Genome Analyzer was used to perform massively parallel 51 bp sequencing in Single End mode. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
identifiers.namespace: GEO
identifiers.id: GSM1563223
attributes.tag: GEO Accession
attributes.value: GSM1563223