alias: GSM4811896
title: GSM4811896: Liver_2_01; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from mouse tissue samples were extracted using TRIzol reagent. Snap frozen tissue samples were thawed and homogenized in the lysing matrix tubes containing TRIzol prior to RNA extraction. mRNAs were isolated using oligo(dT)-attached magnetic beads, fragmented and reverse-transcribed for cDNA synthesis. Adapter-ligated cDNA fragments were PCR amplified and libraries were validated using the Agilent 2100 Bioanalyzer. Qualified double-stranded PCR products were then circularized to produce the final single strand circle DNA library. Libraries were amplified with phi29 to generate DNA nanoballs and were loaded onto BGISEQ-500 DNBseq platform (BGI) flow cells for 100 bp paired end sequencing.
identifiers.namespace: GEO
identifiers.id: GSM4811896
attributes.tag: GEO Accession
attributes.value: GSM4811896