alias: GSM1563368
title: GSM1563368: DCL1KO_C3 [mRNA]; Tetrahymena thermophila; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Protect Cell Mini Kit (Qiagen, Valencia, CA,  cat. # 74624) according to the protocol  in TetraFGD (http://tfgd.ihb.ac.cn/index/smphelp). RNA libraries were prepared for sequencing using standard Illumina protocols. First strand specific libraries were constructed using the Illumina TruSeq Stranded mRNA Sample Prep Kit (RS-122-2101). The Poly-A tailed mRNA as purified using the Sera‐Mag Magnetic Oligo(dT) Beads. The mRNA fragmentation, first strand cDNA synthesis, second strand cDNA synthesis, A tailing, adaptor ligation and PCR amplification were performed according to the stranded mRNA sample preparation guide of the kit.  Sequencing (Single-end (52X1) for C3 and C6, Paired-end (101x2) FOR C10) was performed using an Illumina HiSeq 2000 sequencer (DNA Sequencing Core of University of Michigan).
identifiers.namespace: GEO
identifiers.id: GSM1563368
attributes.tag: GEO Accession
attributes.value: GSM1563368