alias: S136_ONT
title: Nanopore genome sequencing of Streptomyces cyanogenus S136: cell culture
design: S. cyanogenus S136 (DSM5087) was kindly provided by Prof. A. Bechthold (Freiburg University, Germany). Its genomic DNA was purified from 24-h culture grown at 30C in tryptic soy broth according to salting out procedure N4 as described in (Kieser et al. 2000). DNA concentrations and quality were determined using Trinean Xpose (Gentbrugge, Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Bblingen, Germany). The DNA sample that has passed all quality control checks was used to make  WGS library for Oxford Nanopore Technologies (ONT) sequencing (SQK-LSK109 ligation sequencing kit)
identifiers.namespace: SUB9306565
identifiers.id: S136_ONT