alias: GSM4812243
title: GSM4812243: Cut caudal fin_scRNA; Danio rerio; RNA-Seq
library_construction_protocol: Caudal fin amputation was performed with a sterile scalpel under anaesthesia with 0.016% Tricaine (MS222, Sigma) in zebrafish water. Approximately, 150 of regenerating caudal fin (cut) and intact caudal fin (uncut) samples were collected at 24 hours post amputation (hpA) and dissociated into a single-cell suspension. The concentration of freshly dissociated cells was adjusted to 700-800 cells/μl in PBS aiming to capture 4,000 cells by the 10X genomics device. Libraries were constructed according to the manufacturer's instructions using Chromium™ Single Cell 3' v3.1 Reagent Kits and a Chromium instrument.
identifiers.namespace: GEO
identifiers.id: GSM4812243
attributes.tag: GEO Accession
attributes.value: GSM4812243