alias: B1_CM_72_IP
title: Antarctic Mixotrophic Protists
design: chlorophyll maximum water, BrdU labelled bacteria added, 72 hour incubation, immunopreciptitated DNA;; PCR amplification of the V9 region of the 18S ribosomal RNA gene using eukaryote universal primers 1391F and 1510R; the forward primer contained 12 different Multiplex IDs; 3 PCR reactions were performed for each sample, and each reaction was purified using AMPure XP beads; the reactions were pooled and sequenced by the University of Pennsylvania DNA Sequencing Facility using the GS Junior Titanium emPCR Lib-L kit.
identifiers.namespace: SUB1587693
identifiers.id: B1_CM_72_IP