alias: MacMito_W4
title: Macoma balthica Illumina MiSeq mitogenome sequencing, raw paired reads, library W4
design: Total genomic DNA was extracted from foot muscle tissue using the DneasyTM Tissue Kit (Qiagen, Germany) following the manufacturer's protocol and stored at -20 °C until further analyses. Mitogenomes were enriched using long-range PCR. Libraries were prepared using the TruSeq DNA Sample Prep Kits v2 and TruSeq Universal Adapters (Illumina, San Diego, CA, USA). A TruSeq Universal Adapter was used for each DNA library in order to separate reads from different individuals after DNA sequencing. Library sizes were checked on BioAnalyzer chips (Agilent Technologies, Santa Clara, CA, USA). Paired-end library sequencing was carried out on the Illumina MiSeq plateform (2 x 250 bp chemistry) at the GeT-PlaGe lab (GenoToul, Toulouse, France).
identifiers.namespace: University of La Rochelle
identifiers.id: MacMito_W4