alias: PAO1-2
title: RNA-seq of pseudomonas aeruginosa
design: RNA libraries were prepared by NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following manufacturers instruction. Ribosomal RNA were removed by Ribo-zero rRNA Removal Kit. Fragmentation was done using NEB Next First Strand Synthesis Reaction Buffer. cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H). cDNA fragments were selected with AMPure XP beads (Beckman Coulter, Beverly, USA). PCR products were then generated and purified with AMPure XP beads.
identifiers.namespace: SUB7901115
identifiers.id: PAO1-2