alias: qiime_experiment_2151:1170362:153
title: unspecified sequencing; qiime_experiment_2151:1170362:153
design: Skin, oral and fecal samples from Students at CU-Boulder, NAU and NC State U.
library_construction_protocol: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
identifiers.namespace: CCME-COLORADO
identifiers.id: qiime_experiment_2151:1170362:153
identifiers.namespace: Colorado Center for Microbial Ecology, University of Colorado at Boulder
identifiers.id: qiime_experiment_2151:1170362:153
attributes.tag: experiment_title
attributes.value: Flores_SMP
attributes.tag: sample_center
attributes.value: CCME
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: experiment_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 7/7/12
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: run_center
attributes.value: CCME
attributes.tag: num_sequences
attributes.value: 47780
attributes.tag: platform
attributes.value: Illumina
attributes.tag: library_construction_protocol
attributes.value: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: experiment_design_description
attributes.value: Skin, oral and fecal samples from Students at CU-Boulder, NAU and NC State U.
attributes.tag: study_center
attributes.value: CCME
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: row_number
attributes.value: 153