alias: MA-68_S313_L001
title: Targeted mutagenesis with meganuclease treatment
design: PCR products were diluted to 1ng/ul and quarter-volume NexteraXT reactions were performed following the manufacturers protocol (Illumina) with a sample volume of 1.25ul.  Libraries were amplified and barcoded using 12 cycles of PCR with the NexteraXT Index Kit (Illumina) and samples were pooled and quantitated by Bioanalyzer (Agilent) such that approximately 100,000 200-300 bp reads were achieved per sample on a MiSeq sequencer (Illumina).
identifiers.namespace: SUB7857415
identifiers.id: MA-68_S313_L001