alias: CCSK012tumor_T
title: RNA-seq of CCSK tumor: Sample TARGET-51-PALFYG-01A-01R
design: Illumina TruSeq paired-end RNA-seq libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Preparation  kits according to the manufacturer's protocol. Briefly, ribosomal RNA was removed using Ribo-Zero Gold beads. After purification, total RNA was fragmented to 200nt pieces and then reverse-transcribed using reverse transcriptase and random primers.  Second strand cDNA was synthesized using DNA polymerase I and RNase H. These cDNA fragments was added a singl e base and ligated with adaptors. The products were purified and enriched with PCR to create the final RNA-seq libraries.
identifiers.namespace: NCI-KHAN
identifiers.id: CCSK012tumor_T