alias: E-MTAB-6957:074_N1_s
title: Illumina HiSeq 2000 sequencing; RNA profilling of non-small cell lung cancer
design: RNA profilling of non-small cell lung cancer
library_construction_protocol: Study participants and phenotypes Between March 2010 and August 2011, we prospectively recruited newly diagnosed and previously untreated lung cancer patients that were referred for thoracic surgery with curative intent. Subjects were only eligible for inclusion if surgical resection of the tumor occurred prior to any form of medical treatment. Tissue was collected immediately after lobectomy/pneumonectomy. Total RNA was extracted from six 30-μm sections of frozen whole tumor and adjacent lung tissue using the Qiagen-RNeasy Mini kit according to the supplier's instructions (Qiagen, Hilden, Germany). The quantity of DNA-free total RNA was measured using a Nanodrop-2000. Illumina sequencing libraries were prepared according to the TruSeq RNA Sample Preparation Guide following the manufacturer's instructions.
identifiers.namespace: VIB Center for Cancer Biology (CCB)  Laboratory for Translational Genetics  Department of Human Genetics
identifiers.id: E-MTAB-6957:074_N1_s
attributes.tag: Experimental Factor: sampling site
attributes.value: normal tissue adjacent to neoplasm