alias: GSM3406873
title: GSM3406873: WT 3hpf rep1; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was isolated from zebrafish embryos harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO™ DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. Libraries were directly constructed using the KAPA Stranded mRNA-Seq Kit (KAPA) and were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 125 bp.
identifiers.namespace: GEO
identifiers.id: GSM3406873
attributes.tag: GEO Accession
attributes.value: GSM3406873