alias: S. cerevisiae Rep 1 RNA-seq
title: Translation regulatory divergence in yeast
design: Strand-specific RNA-seq library constructed as described in Ingolia et al., Cell 147 2011 789-802. Poly-A RNA from polysome extract was fragmented, ligated to a 3' adapter, and reverse-transcribed.  Resulting cDNA was circularized and PCR amplified 12 cycles to incorporate Illumina library sequences and barcodes
identifiers.namespace: Carnegie Mellon University
identifiers.id: S. cerevisiae Rep 1 RNA-seq