alias: GSM4872355
title: GSM4872355: A98V_aTc0_1; Escherichia coli; Bisulfite-Seq
library_construction_protocol: Genomic DNA were extracted by QIAamp PowerFecal DNA Kit (QIAGEN). The preparation of libraries for shotgun bisulfite sequencing was conducted based on the post-bisulfite adaptor tagging (PBAT) strategy (33) using a modified protocol described recently (tPBAT protocol version 1.0) (34). First, genomic DNA spiked with unmethylated lambda DNA was bisulfite-treated, and random priming using the DNA as a template was performed. After purification of the product DNA with solid-phase reversible immobilization (SPRI) using AMPure XP (Beckman Coulter), the DNA was served for adaptor tagging with TACS-ligation (34). The library structure was completed with two rounds of primer extension using universal and indexing primers. The library was purified with SPRI again, then the molar concentration of the library was determined with a real-time PCR-based method.
identifiers.namespace: GEO
identifiers.id: GSM4872355
attributes.tag: GEO Accession
attributes.value: GSM4872355