alias: GSM4872947
title: GSM4872947: IgG; Mus musculus; RNA-Seq
library_construction_protocol: mDOTS were isolated from the microfluidic device on day 6 of treatment after a 15-minute collagenase treatment. The spheroids were then treated with trypsin in a 37°C incubator for 5 minutes to obtain single cell suspensions. The tumor cells were isolated via FACS, washed, assessed for viability, and loaded onto a 10X Chromium instrument (10X Genomics) per the manufacturer's instructions. Single-cell RNA libraries were generated using the Single Cell 3' Reagent Kit (10X Genomics) per user guide. Quality control of the completed libraries was performed using Bioanalyzer High Sensitivity DNA Kit (Agilent).
identifiers.namespace: GEO
identifiers.id: GSM4872947
attributes.tag: GEO Accession
attributes.value: GSM4872947