alias: GSM1563551
title: GSM1563551: PA14_wildtype_sample1; Pseudomonas aeruginosa UCBPP-PA14; RNA-Seq
library_construction_protocol: Using RNeasy kit (Qiagen) plus Qiashredder columns. Customized protocol (Dötsch et al., PloS ONE, 2012). Briefly, rRNA depletion using MICROBExpress kit with the Pseudomonas module (Ambion) and fragmentation by sonication using Covaris Adaptive Acoustics device (Covaris). Upon ligation of barcoded adapters, reverse transcription with SuperScript II kit (Invitrogen) followed. Amplification was done by PCR using Illumina platform compatible primers. Established cDNA library was normalized by double-strand-specific nuclease treatment (Evrogen) according to DSN Normalization Sample Preparation Guide from Illumina.
identifiers.namespace: GEO
identifiers.id: GSM1563551
attributes.tag: GEO Accession
attributes.value: GSM1563551