alias: GSM4872321
title: GSM4872321: rrp6Δ_replicate1 [s_Empty_MTR4_2]; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cell pellets were washed in sterile nuclease-free water, spun again to remove supernatant and snap-frozen in liquid N2 and saved at -80 °C. Total RNA was isolated from the cell pellets by using MasterPure Yeast RNA Purification kit (Lucigen), resuspended in nuclease-free water and saved at -80 °C. Total RNA samples extracted from above mentioned yeast strains were subjected to RiboGreen quantification and quality control was assayed by Agilent BioAnalyzer 4. 1 µg of total RNA with an RNA integrity number varying from 6.1 to 8.9 underwent ribosomal depletion with the Ribo-Zero Gold rRNA Removal Kit (Yeast) (Illumina catalog # MRZY1306). Libraries were prepared using TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to manufacturer's instructions with 8 cycles of PCR. Samples were barcoded and ran on a HiSeq4000 in a 50bp/50bp paired end run using the HiSeq 3000/4000 SBS Kit (Illumina). On average, 61 million paired reads were generated per sample.
identifiers.namespace: GEO
identifiers.id: GSM4872321
attributes.tag: GEO Accession
attributes.value: GSM4872321