alias: GSM4704553
title: GSM4704553: mouse_Myc_DnTrp53; Mus musculus; RNA-Seq
library_construction_protocol: Cerebellar stem/progenitor cells (Prominin1+ cells) were purified by fluorescence activated cell sorting (FACS) from the cerebella of postnatal day 5–7 (P5–P7) C57BL/6J pups as previously described (see references 1-3). To generate Myc-DnTrp53 or Myc-Gfi1 tumors, cells were infected with viruses produced from pMSCV-MycT58A-IRES-Luc and pMSCV-DNp53-IRES-GFP (for MP tumors) or pMSCV-GFI1-IRES-GFP (for MG tumors). After overnight infection, cells were washed and 10,000 cells were stereotaxically injected into the cerebellum of 6- to 8-week-old NSG mice. Animals were monitored weekly and euthanized when they showed signs of tumor. Tumors were then dissociated and resuspended in NeuroCult medium with proliferation supplement (STEMCELL Technologies) for scRNA sequencing analysis.    1. Pei, Y., et al. HDAC and PI3K Antagonists Cooperate to Inhibit Growth of MYC-Driven  Medulloblastoma. Cancer cell 29, 311-323 (2016).  2. Pei, Y., et al. An animal model of MYC-driven medulloblastoma. Cancer cell 21, 155-167  (2012).  3.Northcott, P.A., et al. Enhancer hijacking activates GFI1 family oncogenes in medulloblastoma. Nature 511, 428-434 (2014). 3' end 10x Genomics library prep (v2 and v3)
identifiers.namespace: GEO
identifiers.id: GSM4704553
attributes.tag: GEO Accession
attributes.value: GSM4704553