alias: AtTR_MaPseq_2
title: DMS MaPseq of Arabidopsis thaliana telomerase RNA: 4 day old seedlings
design: 4-day-old wild type (WS ecotype) seedlings were treated with 1% DMS in DMS reaction buffer (40 mM HEPES pH7.5, 100 mM KCl and 0.5 mM MgCl2). 7.5 min of vacuum incubation was applied twice with a thorough mix in between.After TGIRT reaction, AtTR and ACT2mRNA wasamplified and purified PCR productswere directly assembled into Illumina sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit.Libraries were sequenced throughan 150x2 Illumina NextSeq 500 platform
identifiers.namespace: SUB6425694
identifiers.id: AtTR_MaPseq_2