alias: E-MTAB-8584:T_002_p
title: NextSeq 500 paired end sequencing; RNA-seq of human Barrett's oesophagus and oesophageal adenocarcinoma samples
design: RNA-seq of human Barrett's oesophagus and oesophageal adenocarcinoma samples
library_construction_protocol: Barrett's oesophagus and OAC tissue were collected from routine endoscopies and placed into RNALater (Qiagen) and stored at -80degC. Biopsies were minced with a scalpel and RNA was extracted using RNeasy kit (Qiagen). RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit.
identifiers.namespace: UNIVERSITY OF MANCHESTER
identifiers.id: E-MTAB-8584:T_002_p
attributes.tag: Experimental Factor: disease
attributes.value: esophageal adenocarcinoma