alias: SL18158_Pt
title: RNA-Seq of Ptilodexia  rufipennis
design: One male specimen was collected by hand (USA: Ohio: Hocking Co., Crane Hollow Nature Preserve. 17-July-2012. J.O. Stireman III) and placed live in a collecting tube. The thorax from the live specimens was separated and crushed in RNAlater, and stored at 4C. RNA was isolated using a trizol/Chloroform extraction and purified using Qiagen RNeasy spin columns following manufacturers protocol: Purification of Total RNA from Animal Tissues (Qiagen 2006).mRNA samples were prepared using NEB RNASeq reagents utilized with an lab specific protocol (HudsonAlpha). Sequencing was conducted with a TruSeq PE v3 Cluster Kit and TruSeq PE v3 SBS reagents.
identifiers.namespace: SUB6791150
identifiers.id: SL18158_Pt