alias: 13
title: Contrasting fungal responses to wildfire across different ecosystem types
design: DNA was extracted from 0.25g of each soil sample with a Qiagen DNeasy PowerSoil kit according to the manufacturers instructions (Qiagen; Hilden, Germany). The fungal internal transcribed spacer (ITS) region was amplified using polymerase chain reaction (PCR) in a 96-well plate using modified version of the ITS1F-KYO1 and ITS2-KYO2 primers (Toju, Yamamoto, Tanabe, Hayakawa, & Ishii, 2016).  Primer modification allowed molecular tags for multiplexing to be attached with a second PCR reaction following Toju, Yamamoto, Tanabe, Hayakawa, & Ishii (2016). Product was verified with gel electrophoresis and then cleaned using a magnetic bead purification method (Talbot et al., 2014). After fluorometric DNA quantification using Qubit (Thermo Fisher Scientific; Waltham, MA, United States), we pooled samples at equimolar concentration and submitted our library for 2 x 300 Illumina MiSeq sequencing at the Stanford Functional Genomics Facility.
identifiers.namespace: SUB8581823
identifiers.id: 13