alias: GSM4716697
title: GSM4716697: JB_74; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was extracted from “amount” of “certain tissue” using “RNA prep method”. 500 ng of total RNA was used in the small RNA protocol with the TruSeq™ Small RNA sample prepkit v2 (Illumina) according to the instructions of the manufacturer. The barcoded libraries were size restricted between 140 and 165bp, purified and quantified using the Library Quantification Kit - Illumina/Universal (KAPA Biosystems) according to the instructions of the manufacturer. A pool of xx libraries was used for cluster generation at a concentration of 10nM using an Illumina cBot. Sequencing of 50 bp was performed with an IlluminaHighScan-SQ sequencer at the sequencing core facility of the IZKF Leipzig (Faculty of Medicine, University Leipzig) using version 3 chemistry and flowcell according to the instructions of the manufacturer. Demultiplexing of raw reads, adapter trimming and quality filtering were done according to Stokowy et al. (PMID: 2462507).
identifiers.namespace: GEO
identifiers.id: GSM4716697
attributes.tag: GEO Accession
attributes.value: GSM4716697