alias: UMB1465_3422-pfc-b3ATAC2_3444-scATACseq_01_L001
title: UMB1465 10x Genomics single cell ATAC sequencing (scATACseq) of prefrontal cortex tissue: Sample UMB1465_3422-pfc-b3ATAC2_3444-scATACseq
design: "scATACseq was performed using the 10X Genomics Chromium Next GEM Single Cell ATAC Reagent Kits v1.1. Fresh frozen human brain tissue was processed to obtain nuclear pellets.  Nuclear pellets were obtained from PFC tissue dissection, homogenization and ultracentrifugation (1h at 30,000rcf), nuclei were re-suspended in diluted nuclei buffer provided by the manufacturer.  20,000 nuclei/experiment were processed for transposition before loading to the 10X Chromium Controller for GEM generation and barcoding, followed by library construction as per manufacturer instructions. The library was submitted for paired-end dual index sequencing in three flow cells of Illumina S1 NovaSeq6000 100 cycles to target ~50,000 reads per nucleus. "
identifiers.namespace: HARVARD-phs001485
identifiers.id: UMB1465_3422-pfc-b3ATAC2_3444-scATACseq_01_L001