alias: GSM1563392
title: GSM1563392: WT_C3 [smallRNA]; Tetrahymena thermophila; ncRNA-Seq
library_construction_protocol: RNA libraries were prepared for sequencing using standard Illumina protocols. Size selected total RNA (<200bp) in C3, C6 and C10 of WT, ∆EZL1 and ∆DCL1 cells were used to the starting materials. Small RNA libraries were constructed using the Illimuna TruSeq Small RNA Sample Prep Kit (RS-200-0012). Total RNA was ligated with 3’ adaptor and 5’ adaptor respectively, and then first strand was synthesized for PCR amplification. The PCR amplification products with library insert size corresponding to ~22nt and ~30nt were selected by gel. Single-end (50X1) sequencing was performed.
identifiers.namespace: GEO
identifiers.id: GSM1563392
attributes.tag: GEO Accession
attributes.value: GSM1563392