alias: GSM4932175
title: GSM4932175: Bulk RNA-seq dataset: PC9 9-day gefitinib-treated replicate #3; Homo sapiens; RNA-Seq
library_construction_protocol: For the single-cell RNA-seq datasets: Cells for each sample set were pooled from three 100 mm tissue culture plates, following drug treatment for the indicated time.Cells were detached with TrypLE and a single cell suspension of viable cells was prepared. mRNA from each individual cell was captured and tagged with barcodes using 10X Genomics microfluidic platform.  For the bulk RNA-seq datasets: Cell plating was staggered such that RNA from 3-day treated, 9-day treated, and untreated control samples was extracted from cells simultaneously, using RNeasy Mini Kit (QIAGEN) protocol and reagents. For the single-cell RNA-seq datasets: Barcoded cDNA library was prepared using the manufacturer's instructions, 10X Genomics.  For the bulk RNA-seq datasets: Library preparation was performed using the KAPA mRNA HyperPrep Kit. An Agilent Bioanalyzer was used to verify the RNA concentration and assess RNA sample quality.
identifiers.namespace: GEO
identifiers.id: GSM4932175
attributes.tag: GEO Accession
attributes.value: GSM4932175