alias: GSM4103038
title: GSM4103038: Stard5-EGFP/Rpl10a-JX5852, Ventral striatum, IP Rep2; Mus musculus; RIP-Seq
library_construction_protocol: The ventral striatum was dissected and homogenized and tagged polysomes were immunopurified with monoclonal anti-EGFP antibodies bound to biotinylated-Protein L (Thermo Fisher) coated streptavidin-conjugated magnetic beads (Life Technologies). Total RNA from IPs was purified with the Agilent Nanoprep kit following manufacturer's protocol. Ribosomal-bound RNA was extracted and treated as described in PMID 24810037, Heiman M, Kulicke R, Fenster RJ, Greengard P, Heintz N. (2014) Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nat Protoc. 9:1282-1291
identifiers.namespace: GEO
identifiers.id: GSM4103038
attributes.tag: GEO Accession
attributes.value: GSM4103038