alias: C.cellulolyticum_DOP1
title: Amplification of C. cellulolyticum genomic DNA using primer DOP1 and NEB Taq polymerase for generation 1 DOP products
design: Purified genomic DNA was amplified using primer (5’-TAG ACA ATG GGG CAT NNN NNN NNN ATG-3’)using standard Taq polymerase and fragments between 1 and 3kb in size were extracted from an agarose gel. DNA was sheared to 220bp and sequenced in Paired End mode on Illumina MiSeq.
identifiers.namespace: Virginia Polytechnic Inst & St
identifiers.id: C.cellulolyticum_DOP1