alias: LR_PCR_MeiosisGene
title: Long Range PCR ASY1 ASY3 ZYP1 HEI10
design: To sequence ASY1, ASY3, ZYP1 and HEI10 from a larger set of individuals, we amplified the genes by long-range PCR using Phusion High Fidelity DNA Polymerase (New England Biolabs) from 42 Pannonian diploids, 21 Carpathian diploids and 113 tetraploids. We used primers spanning the genes and adjacent intergenic regions conserved between A. arenosa and A. thaliana to generate products of 7.1 kb for ASY1, 17 kb for ASY3, 9.5 kb for ZYP1b, and 4.5kb for HEI10. We pooled PCR product, barcoded each sample as in (Peterson et al. 2012), and then sequenced all products together. We aligned reads to the A. lyrata genome (Hu et al. 2011) using STAMPY (Lunter and Goodson 2011).
identifiers.namespace: Harvard University
identifiers.id: LR_PCR_MeiosisGene