alias: GSM4704392
title: GSM4704392: 1uM BPDP 3h rep2; Mus musculus; RNA-Seq
library_construction_protocol: Cultured limb buds were preserved in RNAlater (Qiagen) and RNA was extracted using the Qiagen RNeasy Plus Mini Kit mRNA was purified from 1ug of total RNA using poly-T oligo-attached magnetic beads and fragmented randomly in a fragmentation buffer. First-strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-); second-strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. Double-stranded cDNA was purified using AMPure XP beads. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of the 3' ends of DNA fragments, NEBNext Adaptor was ligated to prepare for hybridization. The library fragments were purified with AMPure XP system. The final library was obtained by PCR amplification and purification of PCR products using AMPure XP beads.
identifiers.namespace: GEO
identifiers.id: GSM4704392
attributes.tag: GEO Accession
attributes.value: GSM4704392