alias: GSM2971838
title: GSM2971838: Input; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP-seq analysis of NRF2 following 2 hrs TCDD exposure was performed using the FactorPath services by Active Motif (Carlsbad, CA) on ~100mg frozen liver sections. livers cut in small pieces were incubated in 1% formaldehyde for 15 min at room temperature. Cross-linking was halted by the addition of 0.125 M glycine and tissue was homogenized by Dounce homogenizer followed by shearing DNA by sonication to achieve an average length of 300 – 500 bp. A total of 30 μg of precleared sheared chromatin with protein A agarose beads was immunoprecipitated using 20 μL of rabbit anti-NRF2 polyclonal IgG (sc-13032; Santa Cruz, Dallas, TX) overnight at 4°C. Chromatin was eluted from the beads with SDS buffer and cross-links reversed by incubation with RNase and Proteinase K followed by an overnight incubation at 65°C. Libraries for sequencing were prepared by Active Motif and sequenced using NextSeq 500 (1 x 75bp).
identifiers.namespace: GEO
identifiers.id: GSM2971838
attributes.tag: GEO Accession
attributes.value: GSM2971838