alias: 66
title: V3-V4 amplicon sequencing of Homo sapiens: Well differentiated OSCC
design: Libraries for V3-V4 amplicon sequencing was prepared using NEBNext Ultra DNA Library preparation kit. The amplicons are subjected to a sequence of enzymatic steps for repairing the ends and tailing with dA-tail followed by ligation of adapter sequences. These adapter ligated fragments are then cleaned up using SPRI beads. Next, the clean fragments are indexed during limited cycle PCR to generate final libraries for paired-end sequencing. The resulting libraries are quantified before loading on the cBot for cluster generation and sequencing on Illumina MiSeq Ten system to generate 2X250 bp sequence reads.
identifiers.namespace: SUB8122360
identifiers.id: 66