alias: Cas9 RNP TE and HDR Analysis
title: eLife Journal, Article Title:"Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery".  Description:  Cas9 RiboNucleoProtein analysis of Total Editing and Homology Directed Repair at EMX1 and DYRK1A loci plus top two predicted off-target sites for each locus.
design: 1-EMX= EMX1 Target locus 2-DYRK= DYRK Target locus 3-EMX= EMX1 off-target 1 (OT1) 4-EMX= EMX1 off-target 2 (OT2) 5-DYRK= DYRK1A off-target1 (OT1) 6-DYRK= DYRK1A off-target2 (OT2) 10, 30, 100pmol= picomol's of Cas9 RiboNucleoProtein nucelofected in 30ul volume in 2x10^5 cells  Two sgRNA's were used; one targeting EMX1 locus, the other targeting DYRK1 locus.
identifiers.namespace: University of California, Berkeley
identifiers.id: Cas9 RNP TE and HDR Analysis