alias: screen_10
title: Co-culture screen 2 readout, cell control, replicate 1
design: Two rounds of PCR were used for sgRNA library readout. PCR #1 used genomic DNA for sufficient coverage of screen, and PCR #2 used 1L of PCR#1 product with custom barcoded primers. Samples were amplified with different barcoded primers and pooled for deep sequencing.
identifiers.namespace: SUB8094858
identifiers.id: screen_10