alias: AMML-2-3
title: RNA-seq of  bovine foetal hepatocyte-derived cell line (BFH12)
design: For each experimental condition (i.e. C, CL, C+AFB1, CL+AFB1) three independent replicates (i.e. independent cell culture experiments) were considered. As a consequence, a total of 12 tagged RNA-seq libraries were prepared using Agilent's SureSelect Strand Specific RNA Library Preparation Kit (Agilent Technologies), and following manufacturer's instruction. Briefly, poly(A) mRNA was purified from 400 ng of total extracted RNA and fragmented using an RNA-seq Fragmentation Mix. First-strand and second-strand cDNA were synthetized and end-repaired. Adenylation of cDNA 3' ends and adaptor ligation were performed. Twelve cycles of PCR were used to amplify and index the adaptor-ligated cDNA library. The PCR products were then purified, size selected using the SPRIselect reagent kit (Beckman Coulter, Brea, CA, USA), and eluted in 21 mL of RNAse free water.
identifiers.namespace: SUB7856316
identifiers.id: AMML-2-3