alias: ATAC_CRXa
title: ATAC-seq: Crx-/- retina
design: ATAC-seq was performed as published in Buenrostro, et al.[Buenrostro et al., 2015] Briefly, retinas were dissected from P14 WT and Crx-/- mice and washed in PBS. Tissue was dissociated at 37C using 2% Collagenase in TESCA buffer for 13 min, and the reaction stopped by the addition of 2X volume of DMEM + 10% FBS. DNase1 (0.5 Units) was added for the final 3 minutes to minimize clumping of cells. Cells were counted with hemocytometer, and 50,000 re-suspended in TD buffer for a 1 hour incubation with TDE1 at 37C. Remaining library prep was performed as published.
identifiers.namespace: SUB3293554
identifiers.id: ATAC_CRXa