alias: DC5-6-4C-13C-6_1
title: DNA-Seq of the undisturbed active layer samples (SIP)
design: Bacterial 16S rRNA genes were PCR-amplified with dual-barcoded primers targeting the V4 region, based on the recommended Earth Microbiome Project (EMP) primers 515F and 806R (Caporaso et al., 2011).  No additional library preparation kits were used, as the libraries were constructed in a single PCR step.
identifiers.namespace: SUB7897395
identifiers.id: DC5-6-4C-13C-6_1