alias: tempo2tanque2
design: Water was sampled by collecting 500 ml of water in each tank and filtering the water through a 0.2 micron membrane.  DNA was extracted using the DNeasy¨ Blood and Tissue Kit from QIAGEN according to the manufacturer's instructions.  The DNA was amplified by PCR using the primers S-D-Arch-0519-a-S-15  (5ÕCAGCMGCCGCGGTAA-3' ) and S-D-Bact-0785-b-A-18 (5Õ-TACNVGGGTATCTAATCC-3Õ ) specific to region V4 of rRNA 16S. We used the TaKaRa Premixª 2.0 to perform the PCR according to the manufacturer's instructions. DNA concentration was quantified on Nanodrop. The sequencing was done on Illumina MiSeq.
identifiers.namespace: SUB1587864
identifiers.id: tempo2tanque2