alias: GSM4812174
title: GSM4812174: Donor5_8_TRA_Th1; Homo sapiens; RNA-Seq
library_construction_protocol: T cell subsets were FACS sorted from fresh PBMC samples from 5 healthy donors. Effector/memory functional subsets were sorted at >98% purity into RLT  537 buffer (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich).  after exclusion of naive CCR7+CD45RA+ were sorted in lysis buffer TCRα and TCRβ cDNA libraries were 569 prepared using a Human TCR Kit (MiLaboratory LLC)  with template switch-based incorporation of UMIs. OTHER: target RNA-seq (RACE)
identifiers.namespace: GEO
identifiers.id: GSM4812174
attributes.tag: GEO Accession
attributes.value: GSM4812174