alias: ena-EXPERIMENT-UCL-08-01-2018-15:43:54:726-3
title: Illumina MiSeq sequencing
design: Tn-Seq to identify E. coli K1 A192PP genes responsible for colonisation or rat small intestine or survival in human serum
library_construction_protocol: DNA extracted from A192PP-Tn5 population. DNA randomly fragmented, and adapter ligated. Tn specific PCR performed, and fragments size selected. PCR amplification to index amplicons for sequencing
identifiers.namespace: UCL
identifiers.id: ena-EXPERIMENT-UCL-08-01-2018-15:43:54:726-3