alias: GSM4704978
title: GSM4704978: Input (total lysate, patient 3); Homo sapiens; RIP-Seq
library_construction_protocol: For immunoprecipitation of hnRNPC RNA complexes, 100 mg tissue from the human heart apex was used for each IP and control sample.After thawing, heart samples were washed, dissected, cross-linked and mechanically homogenized in lysis buffer. After sonication the sample was centrifuged and the supernatant was incubated O.N with antibody-conjugated-magnetic beads. After 3 washes reverse cross-linking was performed and proteins were digested by Proteinase K. RNA was extracted following TRIzol extraction protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM4704978
attributes.tag: GEO Accession
attributes.value: GSM4704978