alias: E-MTAB-5824:med2 med3 med15 kin28-AA plus rapa med17-myc_p
title: NextSeq 500 paired end sequencing; ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
design: ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
library_construction_protocol: Yeast were grown at 30˚C in YPD to A600 of 0.8; rapamycin added to 1 microgram/ml and incubated 1 hr additional ChIP performed by standard methods.  Antibody incubation was overnight at 4 C. The NEB Next Ultra II DNA library prep kit was used according to manufacturer's instructions.
identifiers.namespace: Wadsworth Center, NY State Dept. of Health
identifiers.id: E-MTAB-5824:med2 med3 med15 kin28-AA plus rapa med17-myc_p
attributes.tag: Experimental Factor: genotype
attributes.value: Mat a med3::KanMX med15::KanMX med2::KanMX leu20  met150 ura30 trp1- tor1-1 fpr1::NAT RPL13A-2XFKBP::TRP1 KIN28-FRB::hph srb4-13myc::URA3
attributes.tag: Experimental Factor: compound
attributes.value: rapamycin
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: anti-myc