alias: Ker907-1
title: Tripneustes kermadecensis Ker907 selected mitochondrial Illumina reads
design: Total genomic DNA was extracted from tube feet using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturers instructions. RNase A (Qiagen) was used for  digestion of RNA during DNA extraction. Extracted DNA was quantified using a Qubit 3.0 Fluorometer (Invitrogen) and DNA integrity was checked by amplification of a portion of the mitochondrial COI gene. Library preparation was carried out by MacroGen (Seoul, Korea) using a TruSeq DNA PCR-Free library preparation kit (Illumina) following the manufacturers protocol for 350 bp insert size. Samples were barcoded with TruSeq Dual Indexing reagents (Illumina) to enable pooled sequencing. Library quality control was carried out using a Bioanalyzer system (Agilient). Sequencing was performed by MacroGen on an Illumina HiSeq X instrument, using a paired-end flow cell and 151 cycles. Files contain selected mitochondrial reads from WGS pool (extracted by Bowtie2 search versus mitochondrial reference assembly  [using parameters -X 1000 --very-sensitive-local --no-unal]); only reads where both mates mapped to mitochondrial reference are included.
identifiers.namespace: SUB5079086
identifiers.id: Ker907-1