alias: NTUCH15
title: Targeted sequencing of NUDT15  gene from WBC of Homo sapiens
design: Total RNA was extracted from blood of samples using TRIzol reagent according to the manufacturers instructions (Invitrogen, Waltham, MA, USA). Complementary DNA (cDNA) was synthesized from germline total RNA using the Maxima First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA). NUDT15 cDNA was amplified using Phusion Hot Start II High-Fidelity PCR Master Mix (Thermo Fisher Scientific) with barcoded primers. The barcoded amplicons were purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) and quantified by Qubit reagent (Thermo Fisher Scientific). Equimolar amounts of each amplicon were pooled and sequenced with 300-bp paired-end sequencing using the MiSeq Reagent Kit v3 (Illumina, San Diego, CA, USA).
identifiers.namespace: SUB7896861
identifiers.id: NTUCH15