alias: GSM4766041
title: GSM4766041: G3_75_CI1457_LA_P9S3_9; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was purified from sorted single cells using RNACleanXP Solid Phase Reversible Immobilization (SPRI) beads (Beckman Coulter). Full-length cDNA amplification of single cells was performed using the SMART-Seq v4 Ultra Low-Input RNA protocol. Amplified cDNA was fragmented using Illumina Nextera XT DNA Library Preparation kits and dual-indexed barcodes were added to each sample. Libraries were validated using an Agilent 4200 Tapestation, pooled, and sequenced at 101 SR on an Illumina HiSeq 3000 to an average depth of 1.67 M reads at the Yerkes NHP Genomics Core (http://www.yerkes.emory.edu/nhp_genomics_core/). The number of single cells sorted for each individual subject and their corresponding PCR plate ID is shown in Supplemental Table 2. We used a modified protocol for plate P2S23 where instead of using the Clontech SMART-Seq v4 kit, we used a version of the SMARter method with Clontech buffers as was previously described (58). The template switching oligos for full-length cDNA synthesis were obtained from Exiqon (Woburn, MA, USA) and the primers for cDNA synthesis from Integrated DNA Technologies (Skokie, IL, USA). The libraries for this plate were also sequenced twice.
identifiers.namespace: GEO
identifiers.id: GSM4766041
attributes.tag: GEO Accession
attributes.value: GSM4766041