alias: GSM1565088
title: GSM1565088: L5p_06 set2∆; Saccharomyces cerevisiae BY4741; RNA-Seq
library_construction_protocol: Total RNA was extracted using the hot acid phenol protocol (2). Poly(A)+-RNA were obtained by two successive rounds of purification using oligo (dT)25 magnetic beads (New England Biolabs) following the manufacturer's protocol. RNAs were incubated with TAP (Tobacco Acid Pyrophosphatase) and biotynilated. After fragmentation the 5'-end RNAs were purified using strepavidin magnetic beads RNA libraries were prepared for sequencing using standard Illumina protocols with only a first step to add a specific tag (NNNNCGCGCGNN or NNNNCGCCGGNN) at the 5'-end of each sequence to eliminates further contamination
identifiers.namespace: GEO
identifiers.id: GSM1565088
attributes.tag: GEO Accession
attributes.value: GSM1565088