alias: GSM3581869
title: GSM3581869: Fto Knockout  RNA-seq rep2; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq, RNA was isolated from was isolated from Liver Tissue using the TRIzol (Invitrogen) reagent by following the company manual. mRNA was isolated by using Dynabeads mRNA Purification Kit (Invitrogen). For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. Approximately 100ng of mRNA was used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer's protocol.The libraries were sequenced using HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 101 bp.
identifiers.namespace: GEO
identifiers.id: GSM3581869
attributes.tag: GEO Accession
attributes.value: GSM3581869