alias: Tel-Shikmona OCT15-18s
title: 18S of shallow eastern Mediterranean coastal waters
design: DNA extractions were done directly from the Sterivex filters as previously described in Raveh et al. (2019), followed by amplification and sequencing of the 16S and 18S rRNA gene regions. Prokaryote 16S rRNA genes (V4V5 hypervariable region) were amplified using the 16S rRNA gene V4 variable region PCR primers 515F (Caporaso et al., 2011). Eukaryotic 18S rRNA genes were amplified using the universal primers Euk-A (Medlin et al., 1988) targeting the gene V3 variable region with barcode on the forward primer in a 28 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA).
identifiers.namespace: SUB8358052
identifiers.id: Tel-Shikmona OCT15-18s