alias: GSM1564182
title: GSM1564182: Math1::Cre; Rosa tdTomato rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Pregnant females were sacrificed at gestational day e18.5 to retrieve mutant and wild type embryos. Embryos were than hand dissected to extract cochlear nuclei. The collected tissue was than incubated with Trypsin 0.5% in DMEM at 37°C while shacking. Trypsin was blocked with DMEM complemented with FBS 10% and cells were mechanically dissociated on ice using a pasteur glass pipet. Cells were washed 3 times in DMEM. 30000 cells per sample were than FACS sorted and collected in Lysis buffer. RNA was extracted using the Picopure extraction kit. For each replicate sample 3 littermate embrios were pulled together. Embryos for each sample come from a different pregnant mother. Libraries were constructed from 2ng total RNA with the TotalScript RNA-seq kit (Epicentre) using a mix of poly-A and random primers and 14 cycles of PCR for the amplification. Each library were barcoded and all 8 libraries pooled equimolarly. The pool was sequenced on 3 different lanes on an Illumina HiSeq2500 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM1564182
attributes.tag: GEO Accession
attributes.value: GSM1564182