alias: GSM3723757
title: GSM3723757: RNAseq_confluentHFF_rep2; Homo sapiens; Toxoplasma gondii RH; RNA-Seq
library_construction_protocol: cytoplasmic lysate was collected and an aliquot was immediately stored in Trizol for RNA-seq samples.  The rest was digested with RNaseI and monosomes were collecte by ultracentrifugation on a sucrose gradient. Ribosome-protected fragments (RFP) were purified after gel electrophoresis RNA-seq and Ribo-seq samples of confluent samples were processed with the TruSeq Ribo Profile kit (Illumina) as per manufacturer. Subconfluent samples: total RNA was fragmented by alkaline hydrolysis. Fragments were purfied by gel electrophoresis. fragments were dephosphorylated, ligated at 5' end with barcoded adapter, rRNA removal with RiboZero mammalian kit (Illumina), phosphorylated, ligated at 3' end with SR-adapter, reverse transcribed & PCR with NEBnext barcoded adapters
identifiers.namespace: GEO
identifiers.id: GSM3723757
attributes.tag: GEO Accession
attributes.value: GSM3723757