alias: CH3
title: Bacterial assemblages associated with the coral Platygyra carnosus healthy tissue
design: The bacterial 16S rRNA genes were amplified by PCR using the FastStart High Fidelity PCR system (Roche Molecular Diagnostics, Branchburg, NJ, USA) with the primers 341F (5’- ACTCCTACGG GAGGCAGCAG-3’) and 926R (5’- CCGTCAATTCMTTTGAGTTT-3’) targeting the V3-V5 region. Both forward and reverse primer were fused to Roche Fusing primer A and B, and each Fusion primer A was followed by a unique 10 bases DNA bar-codes specific for each sample. The amplicons, amplified in duplicates from each DNA sample, were pooled and gel purified using 1% agarose gel and the PureLink* Quick Gel Extraction Kit (Invitrogen Life technologies, HK). The quality and quantity of amplicons were checked with Nanodrop 2000 and PicoGreen assay before sequencing.
identifiers.namespace: Hong Kong Baptist University
identifiers.id: CH3