alias: CK_2
title: Rhynchophorus ferrugineus  gut microbiota by 16S rRNA gene-based Illumina high-throughput sequencing
design: The variable region V3 + V4 region of 16s rDNA was amplified by primer with barcode. The primer sequence used was: 341F: CCTAYGGGRBGCASCAG; 806R: GGACTACNNGGGTATCTAAT. The PCR product was then sequestered and quantified by Qubit3.0 fluorometer (Thermo Fisher Scientific, USA). The purified amplification products were mixed in equal amounts, ligated and sequencing library was constructed
identifiers.namespace: SUB3474186
identifiers.id: CK_2