alias: GSM5163813
title: GSM5163813: NCM 10X sham2; Mus musculus; RNA-Seq
library_construction_protocol: For the isolation and collection of non-cardiomyocytes, hearts were minced and enzymatically dissociated using 2 mg/mL type 2 collagenase (Worthington), 1 mg/mL dispase (Roche), and 20 U/mL DNAse I (Roche), with 5 cycles of digestion for a total 40 min at 37 °C. After using a 40-μm cell strainer (Greiner) to remove the cardiomyocytes, cells were stained with Zombie Green Fixable Viability Kit (BioLegend) and live cells were collected by fluorescence-activated cell sorting (FACS) using a FACSJazz cell sorter (BD Biosciences). Single-cell cDNA libraries were generated using the Chromium 3′ v2 chemistry kit (10x Genomics) according to the manufacturer's instruction.
identifiers.namespace: GEO
identifiers.id: GSM5163813
attributes.tag: GEO Accession
attributes.value: GSM5163813