alias: GSM4872699
title: GSM4872699: 9_RZ_C; Cryptococcus neoformans; RNA-Seq
library_construction_protocol: Cells were then pelleted, flash frozen on dry ice, and lyophilized for ~18 hours. Total RNA was isolated using the Qiagen RNeasy Plant Mini Kit (Qiagen, Valencia, CA); on-column DNase digestion was performed to eliminate contaminating genomic DNA. Total RNA quantity and quality were assessed using the Agilent 2100 Bioanalyzer. Purified RNA was subsequently stored at -80°C. Purified total RNA was processed unenriched or was rRNA was depleted using either Ribo-Zero Yeast kit (Illumina), the NEBNext poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA), or the RNase H method with custom oligos. Ribo-Zero and RNase H treated RNA were then cleaned up with the RNA Clean & Concentrator-5 (Zymo Research) before library preparation.  RNA-Seq libraries were prepared from these enriched samples and from unenriched control samples (i.e. “Unenriched”) using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765) (New England Biolabs, Ipswich, MA).
identifiers.namespace: GEO
identifiers.id: GSM4872699
attributes.tag: GEO Accession
attributes.value: GSM4872699