alias: GSM4716574
title: GSM4716574: Control_Sib_Rep2 [Sib_r2]; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Fly heads were dissected and frozen by liquid nitrogen. Total RNA was isolated from the pooled fly heads at the age of day 50 using RNAzol®RT (Sigma). RNA-sequencing was carried at BGI (Hong Kong) according to company's protocol. RNA quality was assessed with Agilent 2100 bioanalyzer. Following DNAse I treatment, mRNA enriched by magnetic beads coupled to Oligo-dT was fragmented and used as template for cDNA library construction. Short DNA fragments were then subjected to end-repair, A-tailing, adaptor ligation, size selection and sequenced on Illumina HiSeq 4000.
identifiers.namespace: GEO
identifiers.id: GSM4716574
attributes.tag: GEO Accession
attributes.value: GSM4716574