alias: GSM4716569
title: GSM4716569: Control_Sib_Rep1_input; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Fly heads were dissected and frozen by liquid nitrogen. Fly heads were fixed and homogenized for nuclei isolation. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with indicated antibody. ChIP-sequencing was carried at Novogene (Singapore) according to company's protocol. Libraries were prepared according to Illumina's instructions. Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified using KAPA library amplification kit (KK2702) with Illumina primers for 11 cycles and library fragments of ~200-500 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 4000.
identifiers.namespace: GEO
identifiers.id: GSM4716569
attributes.tag: GEO Accession
attributes.value: GSM4716569