alias: GSM4872939
title: GSM4872939: SAN-rep-3-neg; Danio rerio; RNA-Seq
library_construction_protocol: Hearts from transgenic line sqet33mi59BEt were extracted at 72hpf, flash frozen on ice, cells were lysed and sorted into two fractions by FACS and RNA was harvested using Trizol reagent. cDNA synthesis was performed using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TakaRa) was used for cDNA synthesis. RNA-seq libraries were generated by Illumina Nextera XT kit according to manufacturer's protocol. Sequencing libraries were purified with Agencourt AMPure XP PCR purification beads (Beckman Coulter, USA) and fragment distribution was assessed by using High Sensitivity D1000 ScreenTape and Agilent 2200 TapeStation system (Agilent Technologies, USA). KAPA library quantification kit (Kapa Biosystems, USA) was used to quantify the libraries. RNA-seq was performed with paired-end sequencing (2×75bp reads) on the NextSeq 500 (Illumina, USA).
identifiers.namespace: GEO
identifiers.id: GSM4872939
attributes.tag: GEO Accession
attributes.value: GSM4872939