alias: OTU-NmP-I-15_ITS2_16S-515-806
title: ITS2 and 16S V4 amplicon sequencing of soil metagenome in old trial Ukulinga medium nitrogen addition and phosphate addition plot 1
design: Amplicon libraries were prepared using primers targeting the V4 region of the prokaryotic 16S rRNA gene (F-515-GTGCCAGCMGCCGCGGTAA, R-806-GGACTACHVGGGTWTCTAAT) and the fungal ITS2 region (F-ITS4-TCCTCCGCTTATTGATATGC, R-ITS7-GTGARTCATCGAATCTTTG) modified with heterogeneity spacers and Nextera XT kit (Illumina). Indexed products of both PCR lines were pooled. Sequencing was performed on an Illumina MiSeq with 2 x 300 bp.
identifiers.namespace: SUB5093678
identifiers.id: OTU-NmP-I-15_ITS2_16S-515-806