alias: GSM4716006
title: GSM4716006: WT plus Arg; Mycolicibacterium smegmatis; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Mini Kit (Cat# 74106, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set  (Cat#79254, QIAGEN, GmBH, Germany). According to the standard Illumina protocols(rRNA removal, fragmentation, first strand cDNA  and second strand synthesis, terminal repair, 3 'end plus A ligation, enrichment).
identifiers.namespace: GEO
identifiers.id: GSM4716006
attributes.tag: GEO Accession
attributes.value: GSM4716006