alias: SINCLAIR_NGS_00091
title: MEF_ICE_postTAM_RRBS_rep4
design: MspI digested DNA samples were end repaired and A-tailed, then TruSeq Nano DNA LT adaptors were ligated. After clean-up, purified DNA was quantified by qPCR using the Kapa Complete Universal Kit. The libraries underwent bisulfite conversion using the EZ DNA Methylation-Gold Kit and were amplified by PCR. After clean-up, libraries were spiked with 10% phiX and sequenced.
identifiers.namespace: SUB6297500
identifiers.id: SINCLAIR_NGS_00091