alias: FF178
title: 16S region v3v4 metataxonomic sample poultry
design: V3V4 16S rRNA gene amplicon using primers CVI_V3-forw CCTACGGGAGGCAGCAG and CVI_V4-rev GGACTACHVGGGTWTCT. The following amplification conditions were used as previously described : step 1: 98C for 2 min, step 2: 98C for 10 s, step 3: 55C for 30 s, and step 4: 72C for 10 s, step 5: 72C for 7 min. Steps 2 to 4 were repeated 25 times. PCR products were checked with gel electrophoresis, and PE300 sequencing was performed using a MiSeq sequencer (Illumina Inc., San Diego, CA). Primer sequences were trimmed (25nt 5' ends) and analysed following the DaDa2 pipeline. Filtered sequences are deposited.
identifiers.namespace: SUB8425633
identifiers.id: FF178