alias: GSM4716911
title: GSM4716911: CNS_Treg_WT_2; Mus musculus; RNA-Seq
library_construction_protocol: Treg cells were sorted from CNS preps. RNA isolation was performed using MyOne Silane Dynabeads (Thermo Fisher Scientific) followed by fragmentation and barcoding of RNA using 8bp barcodes in conjunction with standard Illumina adaptors. Primers were removed using Agencourt AMPure XP bead cleanup (Beckman Coulter/Agencourt) and samples were amplified with 14 PCR cycles. Libraries were gel purified. RNA-sequencing reactions were performed on  Illumina NextSeq sequencer (Illumina) according to the manufacturer's instructions, sequencing 50bp reads.
identifiers.namespace: GEO
identifiers.id: GSM4716911
attributes.tag: GEO Accession
attributes.value: GSM4716911