alias: GSM1200343
title: GSM1200343: Input N2 L3 Rep1 ChIPSeq; Caenorhabditis elegans; ChIP-Seq
library_construction_protocol: Frozen Larva were broken into pieces using cryo-mixer mill, and crosslinked with 1% formaldehyde for 10 minutes. The worms were collected by centrifugation, were washed with and dounced in FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl). 0.1 sarkosyl was added before sonicating to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of larva extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA were ligated to Illumina or home-made multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.
identifiers.namespace: GEO
identifiers.id: GSM1200343
attributes.tag: GEO Accession
attributes.value: GSM1200343