alias: GSM3409403
title: GSM3409403: 0 mM K+, replicate 2; Mycobacterium tuberculosis; RNA-Seq
library_construction_protocol: RNA was extracted as previously described by Rohde, Abramovitch, and Russell (Cell Host and Microbe 2:352-364, 2007). In brief, RNA was stabilized with guanidine-thiocyanate lysis buffer prior to a freeze-thaw step. Once thawed, bacteria were lysed with 5 µg/ml lysozyme, followed by hot Trizol and physical disruption through silica bead agitation in a Beadbeater.  A chloroform extraction was used to further isolate the RNA before purification with an RNAeasy kit (Qiagen). DNA was degraded with Turbo DNAfree DNAse (Ambion). RNA quality was assessed with an Aligent Bioanalyzer before removal of rRNA with a Ribo-Zero rRNA removal kit (bacterial) (Ilumina). The cDNA library was constructed with the Illumina Tru-Seq Stranded kit. The polyA enrichment procedure was skipped.
identifiers.namespace: GEO
identifiers.id: GSM3409403
attributes.tag: GEO Accession
attributes.value: GSM3409403