alias: BR_W22_BC6_WGBS
title: Whole genome bisulfite sequencing of BR_W22_BC6
design: Genomic DNA was sheared to 200-300bp fragments, which were then subject to end repair, A-tailing, adapter ligation and dual-SPRI size selection using KAPA library preparation kit (KK8232) following manufacturers instructions. The size (250bp  450bp) selected library was then treated with bisulfite to convert unmethylated cytosine to uracil using Zymo EZ DNA methylation lightning kit (D5031). The converted DNA was then PCR amplified using KAPA HiFi HotStart Uracil + (KK2801) with the following program: 95/2min, 8 times of 98/30s, 60/30s, 72/4min, and a final extension at 72 for 10min.
identifiers.namespace: SUB5264529
identifiers.id: BR_W22_BC6_WGBS