alias: axolotl_LB_AM4
title: Ambystoma mexicanum Raw sequence reads,  limb blastema (LB)
design: Axolotl juveniles (Ambystoma mexicanum) were obtained from the Center for Regenerative Therapies (Dresden, Germany) and maintained in accordance with the animal care guidelines at the Museum fr Naturkunde Berlin (Germany). A total of 6 axolotls ranging from 8-12 and 5-8 cm were used in the study. Animals were kept in individual tanks with regular water changes and fed once a day. For limb regeneration studies, axolotls were anesthetized in 0.1% MS-222 (Sigma) and forelimbs were bilaterally amputated at the level of the stylopod. A portion of the stylopod tissue was sampled and labelled as NRL, and regenerating limb tissue was sampled from left and right forelimbs at 14 dpa. All tissues collected were stored in RNAlater (Sigma) for RNA extraction and subsequent RNA-seq experiment.
identifiers.namespace: SUB4285322
identifiers.id: axolotl_LB_AM4