alias: qiita_ptid_5599:12021.BF.24.2
title: Illumina MiSeq sequencing; qiita_ptid_5599:12021.BF.24.2
design: a clinical trial concerning the outcomes of an infant formula supplemented with a bovine MFGM fraction
library_construction_protocol: Sequences were amplified using F515/R806 primer pair to target V4 region of 16S rRNA gene Both forward and reverse primers were modified to contain an illumina adapter region for sequencing on the illumina MiSeq platform and on the forward primer site, an 8 bp Hamming error-correcting barcode was used to enable sample multiplexing.
identifiers.namespace: UCSDMI
identifiers.id: qiita_ptid_5599:12021.BF.24.2
identifiers.namespace: University of California San Diego Microbiome Initiative
identifiers.id: qiita_ptid_5599:12021.BF.24.2
attributes.tag: barcode
attributes.value: ACTGCAGT
attributes.tag: center_name
attributes.value: UC Davis Slupsky Lab
attributes.tag: center_project_name
attributes.value: tumme_project
attributes.tag: linker
attributes.value: GT
attributes.tag: linkerprimersequence
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: pcr_cond
attributes.value: initial: 95 deg C for 5min; denaturation: 98 degC for 20sec; annealing: 55 degC for 15sec; elongation: 72 degC for 60sec; final elongation: 72degC for 10min; 25 total cycles
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGCCAGCMGCCGCGGTAA
attributes.tag: reverseprimer
attributes.value: GGACTACHVGGTWTCTAAT
attributes.tag: run_center
attributes.value: UC Davis Genome Center
attributes.tag: run_date
attributes.value: 4/3/2017
attributes.tag: sequencing_meth
attributes.value: Sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4