alias: GSM4932584
title: GSM4932584: JTL621_EtOH_NHS_8h_R3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted using 300 uL Trizol reagent. Worms were vortexed continuously for 20 minutes at 4°C and then went through one cycle of freeze-thaw to help release RNA. Following this, RNA was purified using Direct-zol RNA MiniPrep kit (Zymo Research) as per manufacturer's instructions using on column DNase I digestion to remove genomic DNA. Total RNAs were polyA enriched, and directional RNA-seq libraries were prepared using NEBNext Ultra II RNA library prep Kit.
identifiers.namespace: GEO
identifiers.id: GSM4932584
attributes.tag: GEO Accession
attributes.value: GSM4932584