alias: GSM4766555
title: GSM4766555: WT_D0_F_30372LU; Mus musculus; RNA-Seq
library_construction_protocol: For stress fracture, a 5 mm section of the ulna was removed surrounding the injury site. Soft tissue was removed, the sample was flash frozen in liquid nitrogen, and stored at −80 °C until RNA extraction. The left ulna was similarly processed to serve as a control.Frozen bone samples were pulverized (Braun Mikrodismembrator), dissolved into TRIzol (Invitrogen), and stored at −80 °C. Later, the TRIzol-sample solution was thawed and processed using an RNA clean-up and concentration kit (Norgen Biotek). RNA concentration was measured (Thermo Scientific Nanodrop ONE) and quality was evaluated (Agilent Technologies Bioanalyzer 2100). Only samples with RINs >6.5, representing acceptable quality, were used in sequencing. Ribo-Zero Gold
identifiers.namespace: GEO
identifiers.id: GSM4766555
attributes.tag: GEO Accession
attributes.value: GSM4766555