alias: pool_33
title: DNA-Seq of Chalmydia trachomatis : pooled random mutants: Sample CTL2M_Pool 33
design: Mutant isolates were grown separately on Vero cells.  C. trachomatis EBs collected from Vero cell lysates were treated with DNase I to eliminate residual host cell DNA.  Total DNA was isolated with a Qiagen DNeasy Blood and Tissue Kit. DNA from 21 mutants (including one control which had been previously sequenced) was pooled in equimolar amounts.  This pooled DNA was used as input for library preparation using the Illumina TruSeq DNA Kit.  Five of these libraries (with differently barcoded adapters) were combined in a single lane on an Illumina HiSeq 2000.
identifiers.namespace: Duke University
identifiers.id: pool_33
attributes.tag: Library_construction_protocol
attributes.value: Illumina TruSeq Kit