alias: GSM4154548
title: GSM4154548: miRNA-seq coculture3; Homo sapiens; miRNA-Seq
library_construction_protocol: miRNA-seq: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for miRNA purification. miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by Gel electrophoresis. RNA-seq: RNA libraries were prepared for sequencing using standard Illumina protocols miRNA-seq: RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM4154548
attributes.tag: GEO Accession
attributes.value: GSM4154548