alias: E-MTAB-8446:siNT rep3_p
title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of OE19 cells treated with siNT or siKLF5 for 72 hours
design: RNA-seq of OE19 cells treated with siNT or siKLF5 for 72 hours
library_construction_protocol: Cells were cultured in 37deg incubator with 5% CO2. Cells were treated with 100pmol siRNA using RNAiMAX for 72 hours.  RNA was collected using Qiagen RNeasy kit. Cells were lysed directly in the plate.  RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit.
identifiers.namespace: UNIVERSITY OF MANCHESTER
identifiers.id: E-MTAB-8446:siNT rep3_p
attributes.tag: Experimental Factor: RNA interference
attributes.value: scrambled siRNA