alias: GSM3722592
title: GSM3722592: MAP_1; Bos taurus; miRNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure Approximately 5 µg of total RNA were used to prepare six small RNA libraries (MAP_1, MAP_2, MAP_3, C_1, C_2, C_3) according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). And then the libraries were sequenced by Illumina Hiseq 2500 at the LC-BIO following the vendor's recommended protocol. Raw reads were subjected to an in-house program, ACGT101-miR (LC Sciences, Houston, Texas, USA) to remove adapter dimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, snoRNA) and repeats.
identifiers.namespace: GEO
identifiers.id: GSM3722592
attributes.tag: GEO Accession
attributes.value: GSM3722592