alias: E-MTAB-7434:Pigmented gut_s
title: NextSeq 500 sequencing; Pseudo-albino or pigmented Solea senegalensis (Senegalese sole) anterior gut and dorsal skin transcriptomes (RNA-seq), raw sequence reads
design: Pseudo-albino or pigmented Solea senegalensis (Senegalese sole) anterior gut and dorsal skin transcriptomes (RNA-seq), raw sequence reads
library_construction_protocol: N=6 pseudo-albino or n=6 normally pigmented juvenile sole (6-month old) belonging to the same spawning batch in Aquaculture (Cadiz, Spain) were anesthetized and dorsal skin and anterior gut samples were excised and incubated in RNA-later at 4ºC for 24 h and stored at -20ºC until RNA extraction. Total RNA was extracted using an E.Z.N.A. Total RNA Kit I (Omega-Biotek) following mechanical disruption of skin and gut tissue fragments using 5 mm iron beads (Qiagen) for 2x20sec at 6,800 rpm in a Precellys 24. RNA samples were treated with DNAse to remove possible DNA contamination. Four RNA-seq libraries were constructed using pools of total RNA from dorsal skin and anterior gut of pseudo-albino or pigmented sole (n=4/pool, 250 ng/individual). RNA-seq libraries were prepared using an Illumina TruSeq RNA sample preparation kit and TruSeq index adaptors.
identifiers.namespace: Centre of Marine Sciences (CCMAR)
identifiers.id: E-MTAB-7434:Pigmented gut_s
attributes.tag: Experimental Factor: phenotype
attributes.value: pigmented