alias: GSM4873072
title: GSM4873072: GBM5 OE Contrl; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted by RNeasy Mini Ki (Qiagen) Total RNA samples were verified for integrity using RNA assays on an Agilent TapeStation instrument. Satisfactory samples (RIN scores were > 9.3) were then subjected to poly-A mRNA enrichment, cDNA synthesis and Illumina compatible library preparation using the NEBNext Poly(A) mRNA Magnetic Isolation Module, the NEBNext Ultra II Directional RNA  Library Prep Kit for Illumina and NEBNext Multiplex Index Primers, all according to the New England Biolabs recommended protocols. Libraries were then quantitated by both Agilent TapeStation High Sensitivity assays and a Kapa qPCR Library quantitation assay before pooling. Sequencing was performed on an Illumina NextSeq 500 sequencer with 75 cycle high-output sequencing kits, as per standard Illumina methods, to yield an average of 70 million reads per sample. 
identifiers.namespace: GEO
identifiers.id: GSM4873072
attributes.tag: GEO Accession
attributes.value: GSM4873072