alias: qiita_ptid_1142:1191.sample.10
title: Illumina HiSeq 2000 sequencing; qiita_ptid_1142:1191.sample.10
design: DNA from stool samples collected from participants after consumption of both a low and high resistant starch diet, either in the context of a low or high carbohydrate diet, will be used for sequencing to identify shifts in microbial communities in response to changes in dietary composition. Microbiome patterns will be compared between responders and non-responders (upper and lower 1/3) for the main clinical outcomes of fasting and postprandial insulin and components of atherogenic dyslipidemia (triglycerides, HDL-cholesterol, small LDL particles) with adjustment for covariates including age, sex, and BMI. We will also explore the possibility that inter-individual diet-independent variation in the gut microbiome may be related to the magnitude of change in metabolic parameters in response to diet.
library_construction_protocol: Illumina HiSeq V4, 515f,806rbc primers
identifiers.namespace: UCSDMI
identifiers.id: qiita_ptid_1142:1191.sample.10
identifiers.namespace: University of California San Diego Microbiome Initiative
identifiers.id: qiita_ptid_1142:1191.sample.10
attributes.tag: barcode
attributes.value: GTCACCAATCCG
attributes.tag: center_name
attributes.value: PNNL
attributes.tag: center_project_name
attributes.value: Dietary crossover
attributes.tag: experiment_center
attributes.value: PNNL
attributes.tag: linker
attributes.value: GT
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: primer
attributes.value: GTGTGCCAGCMGCCGCGGTAA
attributes.tag: run_center
attributes.value: CCME
attributes.tag: run_date
attributes.value: 3/26/12
attributes.tag: run_prefix
attributes.value: Emp_LBNL_diet_NoIndex_L005_R1_001
attributes.tag: samp_size
attributes.value: .1,g
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4