alias: GSM4812271
title: GSM4812271: meningeal_cell_preparation; Mus musculus; RNA-Seq
library_construction_protocol: Mice were perfused with PBS and the top layer of the brain was scraped off with a scalpel. The tissue was dissociated using collagenase / dispase for 30 min at 37°C, treated with myelin-removal beads (MACS Miltenyi, # 130-096-433) and enriched for KIT+ ce Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead kit v3.1 and i7 Mutiplex kit (10X Genomics) according to the manufacturers instructions. Libraries were sequenced on an Illumina HiSeq 2500 using the recommen
identifiers.namespace: GEO
identifiers.id: GSM4812271
attributes.tag: GEO Accession
attributes.value: GSM4812271