alias: GSM1565262
title: GSM1565262: 26sr1; Mus musculus; MBD-Seq
library_construction_protocol: Genomic DNA was extracted using the Gentra Puregene Blood/Tissue Kit (Qiagen, Valencia, CA) following the vendor’s instructions. The DNA was fragmented into a median length of 180 base pairs using ultrasonication with the Covaris S2 instrument (Covaris, Woburn, MA, USA). The amount of starting material for each methylation enrichment reaction was 1.5 µg of DNA for both MethylMiner and MethylCap. The methylation-enriched DNA from MethylMiner and MethylCap, respectively, was used as input materials for barcoded fragment libraries for SOLiD5500xl Wildfire deep sequencing. Each library was sequenced using single-end chemistry at 50 bp read length.
identifiers.namespace: GEO
identifiers.id: GSM1565262
attributes.tag: GEO Accession
attributes.value: GSM1565262