alias: E-MTAB-9161:F1-2_p
title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of VCAM1-positive cardiac fibroblasts (VCF) and VCAM1-negative cardiac fibroblasts (VNCF).
design: RNA-seq of VCAM1-positive cardiac fibroblasts (VCF) and VCAM1-negative cardiac fibroblasts (VNCF).
library_construction_protocol: VCF and VNCF were isolated from fetal human cardiac fibroblast by MACS against VCAM1. Fetal human cardiac fibroblasts were cultured in HFDM-1(+) medium supplemented with 1% (v/v) Newborn Calf Serum (NBCS). After initial expansion, fibroblasts were incubated with a biotin-conjugated anti-CD106 (VCAM1) antibody and anti-biotin microbeads for isolation of VCF and VNCF by MACS. Total RNA was extracted using the RNeasy Plus Mini Kit following the manufacturerâ€™s instructions (Qiagen). The library was constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) by GENEWIZ.
identifiers.namespace: Metcela Inc.
identifiers.id: E-MTAB-9161:F1-2_p
attributes.tag: Experimental Factor: phenotype
attributes.value: VCAM1 negative