alias: GSM1845112
title: GSM1845112: ago3_sh_totsRNAseq; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: smallRNA cloning was performed according to Brennecke et al, Cell, 2007 total RNA was isolated with Trizol. small RNAs were PAGE selected and 3' and 5' linkers was ligated. 1st strand cDNA synthesis was performed using SuperScript III (Invitrogen) and the library was amplified using the Kapa HiFi Library Amp real time Kit (Peqlab) with Illumina TruSeq compatible primers. sizeselected 18-29nt smallRNA cloning
identifiers.namespace: GEO
identifiers.id: GSM1845112
attributes.tag: GEO Accession
attributes.value: GSM1845112