alias: GSM3406668
title: GSM3406668: GRWT2; Danio rerio; Bisulfite-Seq
library_construction_protocol: Whole brains were dissected and genomic DNA was extracted using Qiagen Dneasy Blood and Tissue Kit (Cat No./ID: 69504) according to the manufacturer's instructions. Library prep protocol was optimised at GATC Biotech based on the NEBNext Ultra I DNA Lib Prep Kit for Illumina. This included: fragmentation by sonication, end repair, a-tailing, ligation of methylated adapters, bisulfite conversion, index PCR, size selection by extraction from agarose gel (band at ~350 bp)
identifiers.namespace: GEO
identifiers.id: GSM3406668
attributes.tag: GEO Accession
attributes.value: GSM3406668