alias: GSM4811964
title: GSM4811964: CaMK2a_Strd_IP_biorep2; Mus musculus; RIP-Seq
library_construction_protocol: Hippocampal CA1 tissue was rapidly dissected from mice and subsequently Dounce homogenized; lysates were used directly for immunoprecipitation. All RNA samples (20 ng for CaMK2a; 2.5 ng for PV) with sufficient integrity as analyzed by 2100 Bioanalyzer were SPIA-amplified with the Ovation RNA-seq System V2 (NuGEN). Subsequently, SPIA-amplified cDNA (1 ug) was fragmented to a length of ~400 bp using a Covaris S2 sonicator (Acoustic Wave Instruments). Fragmented cDNA (100 ng) was used to generate Illumina-compatible sequencing libraries using the Ovation Ultralow System V2 (NuGEN). Libraries were sequenced on a NextSeq 500 for 75 bp single-end reads to a depth of 20-40 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM4811964
attributes.tag: GEO Accession
attributes.value: GSM4811964