alias: Cowpea_Leaves_AbsCont_16h_RB3_1
title: RNA-Seq of cowpea leaves under mechanical injury and virus inoculation (CABMV)
design: Total RNA was isolated using the SV Total RNA Isolation System kit (Promega, US) following manufacturer's protocol. Agarose gel (1.5 %) and Agilent 2100 Bioanalyzer (Agilent Technologies, EUA) evaluated both concentration and quality of total extracted RNA. Only samples with RNA integrity number (RIN)  8.0 were sequenced. A RNAm TruSeq Stranded LT-Set A kit (RS-122-2101) (Illumina, San Diego, CA, EUA) was employed in messenger RNA purification and cDNA library construction according to the manufacturer's instructions. Paired-end reads, with 100 pb in length were generated via the Illumina HiSeq 2500 system, using the following kits: HiSeq Rapid PE Cluster Kit v2 (PE-402-4002); SBS Kit v2 (200 Cycle; FC-402-4021); and TruSeq Stranded mRNA LT - Set A (RS-122-2101). All sequencing steps were performed at the Center for Functional Genomics, University of So Paulo (So Paulo, Brazil).
identifiers.namespace: SUB7899246
identifiers.id: Cowpea_Leaves_AbsCont_16h_RB3_1