alias: GSM4766546
title: GSM4766546: LT-HSC low old rep2 [ATAC-seq]; Mus musculus; ATAC-seq
library_construction_protocol: HSPCs from young and old C57BL/6 mice were sorted into 2% BSA (PBS) and directly subjected to a modified ATAC-Seq protocol (PMID: 27526324). Tagmented DNA was pre-amplified using 5 PCR cycles using barcoded Nextera PCR primers and the optimal numbers of cycle (30% of max) was determined by qPCR. After the second PCR, the DNA was purified by a MinElute PCR purification column (Qiagen) and subsequently size-selected by Ampure beads to obtain fragments between 50-1000bp.
identifiers.namespace: GEO
identifiers.id: GSM4766546
attributes.tag: GEO Accession
attributes.value: GSM4766546