alias: besansky-msg-16-2
title: Multiplexed shotgun genotyping of An. merus x An. coluzzii backcross progeny: Sample AnMSG_File4
design: We constructed multiplexed shotgun genotyping libraries as described in Andolfatto 2011, and on this lane pooled individual MSG libraries of backcross progeny of Anopheles coluzzii and Anopheles merus. For multiplexing, each individual mosquito was assigned a unique index identifying the multiplexed library, which is needed for demultiplexing, and one of 48 barcodes identifying individuals. Backcross progeny from this lane are in sequencing set 2. The library names in this sequencing lane and their indices are as follows Library10.1 CAGATC, Library10.2 ACTTGA, Library11.1 GATCAG, Library11.2 TAGCTT, Library12.1 GGCTAC, Library12.2 CTTGTA, Library13.1 ATCACG, Library13.2 CGATGT, Library14.1 TTAGGC, Library14.2 TGACCA, Library15.1 AGTCAA, Library15.2 AGTTCC, Library16.1 ATGTCA, Library16.2 CCGTCC, Library9.1 ACAGTG, Library9.2 GCCAAT.
identifiers.namespace: University of Notre Dame
identifiers.id: besansky-msg-16-2