alias: WT2
title: RNA-seq of the wildtype of Salmonella typhimurium
design: Total RNA was extracted using a total RNA extraction kit (Sangon Biotech, Shanghai, China). The concentration and integrity of RNA were determined by a Qubit 2.0 fluorometer (Invitrogen, CA, USA) and agarose gel electrophoresis. The cDNA was prepared from the total RNA by reverse transcription. PCR was used to create the cDNA library; the thermal cycling conditions for PCR were as follows: 10 min incubation at 98C for predenaturation, followed by 15 cycles of denaturation at 98C for 30 s and annealing plus elongation at 72C for 30 s. The sequencing libraries were generated by the Illumina HiSeqTM system, and 150 bp paired-end reads were obtained at Sangon Biotech Co., Ltd
identifiers.namespace: SUB7681321
identifiers.id: WT2