alias: GSM3272810
title: GSM3272810: Hb_pellet_polyp_1; Heligmosomoides polygyrus bakeri; ncRNA-Seq
library_construction_protocol: Total RNA was treated with RNA 5' Polyphosphatase (Epicenter) following manufacturer's instructions, before library preparation. Total RNA was extracted using a miRNAeasy mini kit (Qiagen) following manufacturer's instructions. RNA was treated with Turbo DNA-free kit (Thermo Fisher) to remove residual DNA. Libraries were prepared using the CleanTag small RNA library prep kit according to manufacturer's instructions, using total RNA from adult worms (30 ng), and sucrose-gradient purified EVs (equivalent 1E10 EVs measured by Nanosight, Malvern). For all samples, 1:12 dilutions of both adapters were used with 18 amplification cycles (TriLink BioTechnologies). Fragments between 140-170bp were size selected and sequenced on an Illumina HiSeq high output v4 50bp SE in Edinburgh Genomics.
identifiers.namespace: GEO
identifiers.id: GSM3272810
attributes.tag: GEO Accession
attributes.value: GSM3272810