alias: E-MTAB-5309:A1160_089_R3_p
title: Illumina HiSeq 2000 paired end sequencing; RNA sequencing of the treatment of Aspergillus fumigatus with the new antifungal drug 089 -SYBARIS
design: RNA sequencing of the treatment of Aspergillus fumigatus with the new antifungal drug 089 -SYBARIS
library_construction_protocol: A. fumigatus conidia were collected from solid Saboraud cultures using PBS+0.1% Tween, avoiding mycelium fragment and hyphae collection.  Conidia were then inoculated in RPMI 1640 medium + 2% glucose at 2.5x105 conidia/ml concentration supplemented with either 0.1mM 089 molecule or an equal volume of DMSO.  Cells were grown for 4 hours in static at 37°C. After 4 hours of growth in static at 37°C, RNA was extracted from flash frozen samples by bead beating with Trizol. For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).
identifiers.namespace: Division of Biomedical Sciences Warwick Medical School University of Warwick
identifiers.id: E-MTAB-5309:A1160_089_R3_p
attributes.tag: Experimental Factor: 089
attributes.value: compound
attributes.tag: Experimental Factor: 0.1
attributes.value: dose