alias: SC-DS88_S46_L002_R1_001
title: B. abortus Bab1_1612 Tn-seq to reveal genes that are synthetically lethal with Bab1_1612
design: The DNA was sheared (~300 bp fragments), cleaned with a standard bead protocol, end-repaired and A-tailed, and a custom double-stranded Y adapter was ligated. The custom adapter was prepared by annealing Mod2_TS_Univ and Mod2_TruSeq as described in Wetmore et al, 2015, mBio. The sheared fragments containing transposons were enriched by PCR using the primers Nspacer_BarSeq_pHIMAR and P7_MOD_TS_index1 using GoTaq Green Master Mix according to the manufacturers protocol in a 100-L volume with the following cycling conditions: 94C for 2 min, 25 cycles at 94C for 30 s, 65C for 20 s, and 72C for 30 s, followed by a final extension at 72C for 10 min. After a second bead cleanup, the library was sequenced using a standard Illumina sequencing primer on an Illumina HiSeq with a 150-bp single-end read.
identifiers.namespace: SUB4579094
identifiers.id: SC-DS88_S46_L002_R1_001