alias: 61
title: SO_7661_53_C
design: About 250 ng of genomic DNA was sheared by adaptive focused acoustics using a Covaris S220 system. The fragments were end-repaired, adenylated and ligated to adaptors as per SureSelect XT library preparation kit protocol. This was purified by HighPrep magnetic beads and then amplified for 10 cycles of PCR using Illumina-compatible primers. In-solution hybridization was performed for 20 hours at 65C. After hybridization, the captured targets were pulled down by biotinylated probe-target hybrids using streptavidin-coated magnetic beads. The magnetic beads were washed and resuspended in 15 l of nuclease free water and amplified by 10 cycles of PCR.
identifiers.namespace: SUB7654083
identifiers.id: 61