alias: GSM3664929
title: GSM3664929: FB490; Homo sapiens; RNA-Seq
library_construction_protocol: Cells at passages 3–4 were used for RNA extraction performed after 24 hr incubation in serum free medium. Total RNA was extracted from human SMC cultures with Trizol® Reagent (Life Technologies), purified by miRNeasy Mini kit® (Qiagen S.A.) according to the manufacturer's instructions. DNase digestion procedure was followed for each sample. Libraries were prepared from 1μg of total RNA using TruSeq Stranded mRNA kit protocol (Illumina®) according to the supplier's recommendations. Briefly, poly-A RNA was isolated using oligo-dT25 attached to magnetic beads, fragmented into ~300 SCP-long pieces using divalent cations under elevated temperature, used as template for double-stranded cDNA synthesis, Illumina adapters ligation and PCR-mediated cDNA library amplification. Sequencing was carried out on Illumina HiSeq4000,
identifiers.namespace: GEO
identifiers.id: GSM3664929
attributes.tag: GEO Accession
attributes.value: GSM3664929