alias: 83
title: SO_8330_45a_A60_1
design: About 300ng of qubit quantified genomic DNA was sheared by adaptive focused acoustics using a Covaris S220 system. The fragments were end-repaired, adenylated and ligated to Illumina adaptors. Adapter-ligated DNA was purified by JetSeq magnetic beads and then amplified for 10 cycles of PCR. In-solution hybridization was performed for 20 hours at 65C. After hybridization, the captured targets were pulled down by biotinylated probe-target hybrids using streptavidin-coated magnetic beads. The magnetic beads were washed and resuspended in 15 l of nuclease free water. The captured DNA libraries were amplified by 10 cycles of PCR. The final PCR product was purified with JetSeq beads.
identifiers.namespace: SUB7654083
identifiers.id: 83