alias: KIc
title: RNA sample of Musa paradisiaca : Yellow sigatoka infected leaf sample
design: RNA extracted from Yellow Sigatoka infected leaf  samples of Kachkal was used for  cDNA library construction. The sequencing library was prepared by random fragmentation of the cDNA sample followed by fragmentation 5' and 3' adapter ligation in a single step reaction. Adapter-ligated fragments were then PCR amplified and purified. The library was loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. The fragments were then amplified into distinct, clonal clusters through bridge amplification. When cluster generation is complete, the templates were sequenced utilizing the Illumina SBS technology platform (Illumina HiSeq 2500).
identifiers.namespace: SUB4540293
identifiers.id: KIc