alias: SSC-ISU-FL-USMARC101740302-muscle-27ac
title: Illumina HiSeq 3000 paired end sequencing; Pig histone modification in fetal muscle
design: ChIP-Seq paired end experiment on an Illumina HiSeq 3000
library_construction_protocol: NEBNext Ultra II DNA Library prep kit for Illumina
identifiers.namespace: IOWA STATE UNIVERSIT
identifiers.id: SSC-ISU-FL-USMARC101740302-muscle-27ac
identifiers.namespace: Iowa State Universit
identifiers.id: SSC-ISU-FL-USMARC101740302-muscle-27ac
attributes.tag: project
attributes.value: FAANG
attributes.tag: assay type
attributes.value: ChIP-seq
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 1
attributes.tag: experimental protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/faang/ftp/protocols/assays/ISU_SOP_Fetalliver_20180915.pdf
attributes.tag: extraction protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/faang/ftp/protocols/assays/ISU_SOP_Fetalliver_20180915.pdf
attributes.tag: library preparation location
attributes.value: DNA facility, Iowa State University
attributes.tag: library preparation date
attributes.value: 2019-05-08
attributes.tag: sequencing location
attributes.value: DNA facility, Iowa State University
attributes.tag: sequencing date
attributes.value: 2019-05-10
attributes.tag: experiment target
attributes.value: histone_modification
attributes.tag: chip protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/faang/ftp/protocols/assays/ISU_SOP_Fetalliver_20180915.pdf
attributes.tag: control experiment
attributes.value: SSC-ISU-FL-USMARC101740302-muscle-input
attributes.tag: chip target
attributes.value: H3K27Ac
attributes.tag: chip antibody provider
attributes.value: Diagenode
attributes.tag: chip antibody catalog
attributes.value: H3K27ac (Diagenode, catalog: C15410196)
attributes.tag: chip antibody lot
attributes.value: A1723-0041D
attributes.tag: library generation max fragment size range
attributes.value: 600
attributes.tag: library generation min fragment size range
attributes.value: 200