alias: GSM2197888
title: GSM2197888: r873sS-D2_hs_rna_sr_Wiese_C_2; Homo sapiens; RNA-Seq
library_construction_protocol: High Pure RNA Isolation Kit according to manufacturers instructions Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA according to Illumina's instructions. Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega). The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq2000  following the manufacturer's protocols.
identifiers.namespace: GEO
identifiers.id: GSM2197888
attributes.tag: GEO Accession
attributes.value: GSM2197888