alias: AD_1444:PIN1:chr19:9849162:C>T_Bulk_HIF
title: TASeq of Human
design: Primers were  designed with the Primer3 algorithm and were synthesized from Macrogen  (Seoul, Korea). Amplicons were prepared by two-step-PCR using Illumina TruSeq  adapters. First, PCR reactions were carried out using 10 ng of initial  template gDNA. Then, 1st-amplicons were analyzed on 2% agarose gels and the  bands with expected size were isolated and purified using Mega quick-spin kit  (iNtRON, Korea). Next, 100 ng of purified 1st-amplicons were used as  templates for second-PCR, and the products were subsequently purified with  the aforementioned kit. 2nd-amplicons were quantified using Bioanalyzer 2100  (Agilent).
identifiers.namespace: SUB5437198
identifiers.id: AD_1444:PIN1:chr19:9849162:C>T_Bulk_HIF