alias: qiime_experiment_2401:1269503:417
title: unspecified sequencing; qiime_experiment_2401:1269503:417
design: fecal samples from TwinsUK MiSeq runs
library_construction_protocol: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
identifiers.namespace: CCME-COLORADO
identifiers.id: qiime_experiment_2401:1269503:417
identifiers.namespace: Colorado Center for Microbial Ecology, University of Colorado at Boulder
identifiers.id: qiime_experiment_2401:1269503:417
attributes.tag: experiment_title
attributes.value: TwinsUK Miseq
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: sample_center
attributes.value: Kings College London
attributes.tag: sequencing_meth
attributes.value: sequencing by synthesis
attributes.tag: run_date
attributes.value: 2/2/13
attributes.tag: experiment_center
attributes.value: Cornell
attributes.tag: pcr_primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
attributes.tag: region
attributes.value: V4
attributes.tag: run_center
attributes.value: Cornell
attributes.tag: num_sequences
attributes.value: 75829
attributes.tag: platform
attributes.value: Illumina
attributes.tag: library_construction_protocol
attributes.value: This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
attributes.tag: experiment_design_description
attributes.value: fecal samples from TwinsUK MiSeq runs
attributes.tag: row_number
attributes.value: 417
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: study_center
attributes.value: Cornell