alias: GSM4102057
title: GSM4102057: IWP2-d8-Input; Homo sapiens; ChIP-Seq
library_construction_protocol: Neural progenitor cell lysates were clarified from sonicated nuclei and SOX1 complexes were immunoprecipitated with SOX1 antibody (R&D AF3369, respectively. Protein A and G Magenetic Dynabeads were used to capture antibody bound protein-DNA complexes. The ChIP-enriched DNA samples were treated with end-repair of the DNA, adding “A” bases to the DNA, ligating sequencing adapters to DNA fragments, amplifying adapter-modified DNA by PCR and gel purification for ChIPseq using NEBNext Ultra DNA Library Prep Kit for Illumina. Purified libraries were sequenced on Illumina HiSeq X-ten platforms.
identifiers.namespace: GEO
identifiers.id: GSM4102057
attributes.tag: GEO Accession
attributes.value: GSM4102057