alias: GSM3273189
title: GSM3273189: SP_3; Saccharina japonica; Bisulfite-Seq
library_construction_protocol: A total amount of 5.2 microgram genomic DNA be coped with RNAase and spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer's instructions. Then these DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research), before the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X). Library concentration was quantified by Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system
identifiers.namespace: GEO
identifiers.id: GSM3273189
attributes.tag: GEO Accession
attributes.value: GSM3273189