alias: SL18155_Cy
title: RNA-Seq of Cylindromyia  interrupta
design: Two female specimens were collected by hand (USA: Ohio: Hocking Co., Stump Run Rd. 26-May-2012. J.O. Stireman III) and placed live in a collecting tube. Thoraxes from live specimens were separated and crushed in RNAlater and stored at -80C. RNA was isolated using a trizol/Chloroform extraction and purified using Qiagen RNeasy spin columns following manufacturers protocol: Purification of Total RNA from Animal Tissues (Qiagen 2006).mRNA samples were prepared using NEB RNASeq reagents utilized with an lab specific protocol (HudsonAlpha). Sequencing was conducted with a TruSeq PE v3 Cluster Kit and TruSeq PE v3 SBS reagents.
identifiers.namespace: SUB6791150
identifiers.id: SL18155_Cy