alias: CBS-BLKP89
title: Pyrosequencing analysis of bacterial diversity and community composition using 16S rRNA gene V1-V3 region as the target-CBS-BLKP89
design: "The total community DNA was extracted from soils by modified hot SDS-lysis method. Universal primers 27F and barcoded-518R were used for PCR amplification of the V1?V3 hypervariable regions of 16S rRNA genes. PCR products were quantified, purified and sequenced by Roche 454 GS-FLX Titanium for assessment of bacterial diversity and community structure. "
identifiers.namespace: Institute of Applied Ecology, Chinese Academy of S
identifiers.id: CBS-BLKP89