alias: E-MTAB-7753:Sample 4-replicate2_p
title: Illumina HiSeq 2500 paired end sequencing; RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase
design: RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase
library_construction_protocol: cell pellets from 50 ml culture were collected by centrifugation at –4 °C and 9,000 rpm (Hitachi CF16RXII, Qingdao, China) for 5 min. Afterwards, pellets were frozen in liquid nitrogen and stored at–80°C. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA synthesis kit (Invitrogen) according to the manufacturer's instructions.It was performed by Oebiotech (Shanghai, China)   The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. This part of experiment is carried out at Oebiotech (Shanghai, China)
identifiers.namespace: Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
identifiers.id: E-MTAB-7753:Sample 4-replicate2_p
attributes.tag: Experimental Factor: growth condition
attributes.value: stationary phase
attributes.tag: Experimental Factor: time
attributes.value: 96 h