alias: L001131
title: H1 Clone for multi patient influenza study: Replicate 3
design: This control gene amplicon was generated from a signle clonally derived plasmid with the HA gene. Selected PCR products derived from influneza viral samples were cloned using TOPO TA cloning kits and transformed into E. Coli. Colonies were purified to an individual clone. An individual plasmid was subject to sanger sequencing to confirm the HA gene sequence. The colon-purified plasmid was used as template for RT-PCR using custom primers for the AH gene. PCR products were purified using Qiagen PCR purification kits. Sequencing libraries were prepared with the Nextera XT Sample prep kits and Nextera XT 24 index kit. 
identifiers.namespace: Stanford University
identifiers.id: L001131
attributes.tag: alignment_software
attributes.value: BWA 0.6.2