alias: G. subterraneus methylation
title: Geoalkalibacter subterraneus base modification detection and motif analysis
design: Approximately 15 ug of high molecular weight gDNA was extracted using the Qiagen DNeasy Blood & Tissue Kit from early stationary phase cells grown with 30 mM acetate as the electron donor and 30 mM nitrate as the electron acceptor. gDNA was sheared and prepared for PacBio long read sequencing using standard protocols for 20 kb inserts. SMRT bell templates were size selected with a 4-kb cutoff using the Blue Pippin system (Sage Science). Raw reads were generated on the PacBio RS II instrument using P4-C2 chemistry and 180-minute movies.
identifiers.namespace: University of Minnesota
identifiers.id: G. subterraneus methylation