alias: 22
title: SO_6606_146_a_S18
design: About 250 ng of genomic DNA was sheared using Covaris S2 sonicator to generate fragment from 200 bp to 500 bp. Fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode adapters. This was purified with Ampure PCR beads and amplified for 10 cycles of PCR using Illumina-compatible primers. Prepared libraries were hybridized to target probes. The fragments were isolated by magnetic capture using DynabeadsTM MyOneTM Streptavidin T1. Captured library beads were re-suspended in 15l of nuclease free water. Indexing and PCR amplification (11 cycles). Ampure PCR beads were used for cleanup of PCR product.
identifiers.namespace: SUB7654083
identifiers.id: 22