alias: E-MTAB-8568:CP-A input_p
title: Illumina HiSeq 4000 paired end sequencing; KLF5 ChIP-seq in CP-A and OE19 cells
design: KLF5 ChIP-seq in CP-A and OE19 cells
library_construction_protocol: OE19 cells were grown in RPMI media supplemented with 10% FBS and 1% PenStrep. CP-A cells were grown in KSFM supplemented with 5ug/L EGF and 50 mg/L BPE, 10% FBS and 1% PenStrep. 10 million cell per IP were collected. ChIP was carried out as described in Britton E, Rogerson C, Mehta S, Li Y, Li X, Fitzgerald RC, Ang YS, Sharrocks AD. Open chromatin profiling identifies AP1 as a transcriptional regulator in oesophageal adenocarcinoma. PLoS Genet. 2017;13(8):e1006879  ChIP-seq DNA library was prepared using Illumina TruSeq ChIP Library Prep Kit.
identifiers.namespace: UNIVERSITY OF MANCHESTER
identifiers.id: E-MTAB-8568:CP-A input_p
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: input DNA