alias: siMock_untr_1
title: siMock_untr
design: For the library preparation, the quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a Bioanalyzer 2100 (Agilent). Only those samples with a 260 nm/280 nm ratio between 1.82.1 and a 28S/18S ratio within 1.52 were further processed. The TruSeq RNA Sample Prep Kit v2 (Illumina) was used in the subsequent steps. Briefly, total RNA samples (100-1000 ng) were poly-A enriched and then reverse-transcribed into double-stranded cDNA. The cDNA samples were fragmented, end-repaired and polyadenylated before ligation of TruSeq adapters containing the index for multiplexing. Fragments containing TruSeq adapters on both ends were selectively enriched with PCR.  The quality and quantity of the enriched libraries were validated using Qubit (1.0) Fluorometer and the Caliper GX LabChip GX (Caliper Life Sciences). The product is a smear with an average fragment size of approximately 260 bp. Library DNA concentrations were normalized to 10 nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.
identifiers.namespace: SUB7120711
identifiers.id: siMock_untr_1