alias: GSM3722730
title: GSM3722730: K562-dCas9-KRAB_5K-sgRNAs_Batch-1_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were collected, washed once with PBS. Then the cells were resuspended in PBS containing 0.05% BSA to the desired concentration and immediately load on the 10X Chromium controller for the single-cell RNA-seq preparation. For single-cell library preparation, we follow the standard protocol of 10X Chromium 3' V2 kit. After the cDNA amplification, 20µg of samples were separated for the sgRNA enrichment library preparation.
identifiers.namespace: GEO
identifiers.id: GSM3722730
attributes.tag: GEO Accession
attributes.value: GSM3722730