alias: GSM4767217
title: GSM4767217: ATACSeq_NDC_iPSCNeuron_rep5; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-Seq transposition experiments were performed as previously reported on 50,000 NDC (n = 5), PSEN1M146L (n = 2), PSEN1H163R (n = 3), PSEN1A246E (n = 2), and PSEN1A431E (n = 3) hiPSC-derived neurons using the Illumina Nextera DNA Sample Preperation kit (Illumina, Cat. 15028523) and Qiagen MinElute PCR Purification kit (Qiagen, Cat. 28004). ATAC-Seq libraries were generated from transposed DNA using the Kapa Biosystems Real-Time Library Amplification kit (Kapa Biosystems, Cat. 07959028001) according to the manufacturer's recommendations, monitoring amplification by qPCR and stopping the reaction when all samples reached a fluorescence amplification intensity between standards 1 and 3. ATAC-Seq libraries were further purified using the Qiagen MinElute PCR Purification kit and sequenced at the UC San Diego IGM sequencing core on an Illumina HiSeq4000 platform generating Paired-End, 50bp reads with an average of 25 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM4767217
attributes.tag: GEO Accession
attributes.value: GSM4767217