alias: GSM1565289
title: GSM1565289: LaminB1_ChIPSeq_Heart_E9.5_3; Mus musculus; ChIP-Seq
library_construction_protocol: Protocol as previously described (PMID: 23934658). Briefly, whole hearts microdissected on ice, fixed in 1.1% formaldehyde x 10 min, pooled in lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 1% NP-40, 1% SDS, 0.5% DOC), sheared with Diagenode Bioruptor, IP overnight with Protein G Dynabeads, 2ug antibody, washed 5x in RIPA, eluted into TE, incubated at 65°C x 16hr and DNA purified by phenol-chloroform extraction. Library prepared with NEBNext Ultra DNA Library Prep kit for Illumina according to manufacturer instructions
identifiers.namespace: GEO
identifiers.id: GSM1565289
attributes.tag: GEO Accession
attributes.value: GSM1565289