alias: GSM4716604
title: GSM4716604: TSS_2016_2_DNA; Saccharomyces cerevisiae; OTHER
library_construction_protocol: For DNA, plasmids were isolated using Qiagen kits (Qiaprep Spin Miniprep #27106, or Plasmid Plus Midi). Total RNA was extracted using a Zymo Research Fungal/Bacterial RNA Miniprep kit (ZR, #R2014) or a Qiagen RNeasy Maxi Kit. For some samples, polyA-RNA was isolated using an mRNA Purification kit (Ambion #61006). Sequencing libraries were constructed by PCR targeted at the barcodes. For RNA samples, 1st-strand cDNA was created using a targeted primer and used as the template for the PCR.
identifiers.namespace: GEO
identifiers.id: GSM4716604
attributes.tag: GEO Accession
attributes.value: GSM4716604