alias: GSM1565105
title: GSM1565105: LT_02 upf1∆xrn1∆; Saccharomyces cerevisiae BY4741; RNA-Seq
library_construction_protocol: Total RNA was extracted using the hot acid phenol protocol (2). Poly(A)+-RNA were obtained by two successive rounds of purification using oligo (dT)25 magnetic beads (New England Biolabs) following the manufacturer's protocol. RNAs were incubated with TAP (Tobacco Acid Pyrophosphatase) and biotynilated. After fragmentation and elimination of the 5'end RNAs with magnetic beads, POLY(A) RNAs were also removed from the supernatant using oligo (dT)25 magnetic beads. the resultant supernatant was used as body RNAs source RNA libraries were prepared for sequencing using standard Illumina protocols with only a first step to add a specific tag (NNNNCGCGCGNN or NNNNCGCCGGNN) at the 5'-end of each sequence to eliminates further contamination
identifiers.namespace: GEO
identifiers.id: GSM1565105
attributes.tag: GEO Accession
attributes.value: GSM1565105