alias: SL18161_Ch
title: RNA-Seq of Chrysotachina  longipennis
design: One female specimen was collected by hand (USA: West Virginia: Hampshire Co., Springfield, nr. S. branch Potomac River.8-July-2012. J.O. Stireman III) and placed in a collecting tube. The specimen was stored at 4C refrigerator for one day. After this the thorax was taken from the live specimen and crushed in RNAlater and stored at 4C. RNA was isolated using a trizol/Chloroform extraction and purified using Qiagen RNeasy spin columns following manufacturers protocol: Purification of Total RNA from Animal Tissues (Qiagen 2006).mRNA samples were prepared using NEB RNASeq reagents utilized with an lab specific protocol (HudsonAlpha). Sequencing was conducted with a TruSeq PE v3 Cluster Kit and TruSeq PE v3 SBS reagents.
identifiers.namespace: SUB6791150
identifiers.id: SL18161_Ch