alias: N20_12
title: MiSeq of 16S amplicons from human placenta
design: Amplification and sequencing of the V4 region of the 16S rRNA gene was performed using a dual indexing sequencing strategy. Sequencing was done on the Illumina MiSeq platform using a MiSeq Reagent Kit V2 500 cycles (Illumina MS102-2003). AccuPrime High Fidelity Taq (Life Technologies 12346094) was used. Sequencing libraries were prepared according to Illuminas protocol for Preparing Libraries for Sequencing on the MiSeq (15039740 Rev. D) for 2nM or 4nM libraries. FASTQ files were generated for paired end reads.
identifiers.namespace: SUB4572256
identifiers.id: N20_12