alias: GSM3664802
title: GSM3664802: Murine pancreatic cancer cell line KPC-NC_LoF3; Mus musculus; RNA-Seq
library_construction_protocol: For all cells, RNA was extracted using Qiagen RNeasy columns. poly(A)-selected, unstranded Illumina libraries were prepared with a modified TruSeq protocol. 0.5× AMPure XP beads were added to the sample library to select for fragments <400 bp, followed by 1× beads to select for fragments >100 bp. These fragments were then amplified with PCR (15 cycles) and separated by gel electrophoresis (2% agarose). 300 bp DNA fragments were isolated and sequenced on the Illumina HiSeq 2000 (~100M 2×49 bp reads per sample).
identifiers.namespace: GEO
identifiers.id: GSM3664802
attributes.tag: GEO Accession
attributes.value: GSM3664802