alias: GSM3665049
title: GSM3665049: IRF9KO THP rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed twice with cold PBS. For total RNA isolation, 7 ml Qiazol Lysis Reagent (Qiagen) were added per 15 cm dish. 1ml of the ysate was used for RNA isolation. For DNase treatment and clean up the RNase-free DNase Set (Qiagen, #79254) and RNeasy Mini Kit (Qiagen, #74104) were used. For library preparation, the NEBNext® Poly(A) mRNA Magnetic Isolation Module together with the NEBNext Ultra II RNA Library Prep Kit from NEB #E7770L was used according to the manufacturer's protocol. The RNA quality was assessed using Agilent RNA 6000 Nano Assays that were analyzed on an Agilent 2100 Bioanalyzer and all samples displayed a RIN>9.
identifiers.namespace: GEO
identifiers.id: GSM3665049
attributes.tag: GEO Accession
attributes.value: GSM3665049