alias: E-MTAB-5824:med18 kin28-AA plus rapa med17-myc rep2_p
title: NextSeq 500 paired end sequencing; ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
design: ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
library_construction_protocol: Yeast were grown at 30˚C in YPD to A600 of 0.8; rapamycin added to 1 microgram/ml and incubated 1 hr additional ChIP performed by standard methods.  Antibody incubation was overnight at 4 C. The NEB Next Ultra II DNA library prep kit was used according to manufacturer's instructions.
identifiers.namespace: Wadsworth Center, NY State Dept. of Health
identifiers.id: E-MTAB-5824:med18 kin28-AA plus rapa med17-myc rep2_p
attributes.tag: Experimental Factor: genotype
attributes.value: MATalpha, ade2-1, his3-11,15, leu2-3,112, trp1-1, ura3-1, can1-100 tor1-1, fpr1::NAT, RPL13A-2xFKB12::TRP1, KIN28-FRB::HIS3 med17-myc::TRP1 med18::KanMX
attributes.tag: Experimental Factor: compound
attributes.value: rapamycin
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: anti-myc