alias: GSM2199175
title: GSM2199175: RTf12_LPS; Macaca mulatta; RNA-Seq
library_construction_protocol: We lysed and discarded the red cells from the remaining cell pellet by applying red blood cell lysis buffer (RBC lysis solution, 5 Prime Inc.) for 10 minutes followed by centrifugation. We lysed the remaining white blood cells in Qiazol for storage at -80°C, and extracted total RNA from each sample using the Qiagen miRNAEASY kit. Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra RNA Library Prep Kit. Briefly, we purified the poly-adenylated mRNA from 200 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module. The mRNA was then reverse transcribed into cDNA, ligated to Illumina adapters, size-selected for a median size of ~350 bp, and amplified via PCR for 13 cycles. We tagged each sample with a unique molecular barcode and pooled 10-12 samples per Illumina HiSeq 2500 lane of single-end 100 bp sequencing.
identifiers.namespace: GEO
identifiers.id: GSM2199175
attributes.tag: GEO Accession
attributes.value: GSM2199175