alias: E-MTAB-5312:SR3_s
title: Illumina HiSeq 2000 sequencing; Strobilurins as growth-promoting compounds: How Stroby regulates Arabidopsis leaf growth
design: Strobilurins as growth-promoting compounds: How Stroby regulates Arabidopsis leaf growth
library_construction_protocol: Seeds were stratified and germinated in soil for three days in the presence or absence of Stroby, after which they were watered twice a week with or without Stroby until 17 DAS. Plants were grown in soil at 21 ̊C under a 16-h day (80-100 µmol m-2 s-1) and 8-h night regime.  Stroby® WG (BASF), a commercially available strobilurin consisting of 50% (w/w) KM as active ingredient and 50% surfactants, was dissolved in water and used as source of KM at a concentration of 10-9 M. Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). Briefly, polyA-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using a 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina). RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant Mini Kit (Qiagen)
identifiers.namespace: DEPARTMENT OF PLANT BIOTECHNOLOGY AND BIOINFORMATICS, GHENT UNIVERSITY, GENT, BELGIUM DEPARTMENT OF PLANT SYSTEMS BIOLOGY,VIB, GENT, BELGIUM
identifiers.id: E-MTAB-5312:SR3_s
identifiers.namespace: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
identifiers.id: E-MTAB-5312:SR3_s
attributes.tag: Experimental Factor: kresoxim-methyl
attributes.value: compound
attributes.tag: Experimental Factor: 1
attributes.value: dose