alias: GSM4766486
title: GSM4766486: Bulk_RNA-Sequencing of Wild Type or  Tracf/f mice 4; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells (300-800 cells) were lysed in a guanidine thiocyanate buffer (TCL buffer, Qiagen) supplemented with 1% β-mercaptoethanol. RNA was isolated by solid-phase reversible immobilization bead cleanup using RNAClean XP beads (Agentcourt, A63987), reversibly transcribed, and amplified as described (Trombetta et al., 2014). Uniquely barcoded libraries were prepared using Nextera XT kit (Illumina) following manufacturer's instructions. Sequencing was performed on an Illumina NextSeq500 for a total yield of 400M reads.  
identifiers.namespace: GEO
identifiers.id: GSM4766486
attributes.tag: GEO Accession
attributes.value: GSM4766486