alias: E-MTAB-9414:s10_s
title: Illumina HiSeq 2500 sequencing; Transcriptome Profiling of Human Monocyte-Derived Macrophages Upon CCL2 Neutralization Reveals an Association Between Activation of Innate Immune Pathways and Restriction of HIV-1
design: Transcriptome Profiling of Human Monocyte-Derived Macrophages Upon CCL2 Neutralization Reveals an Association Between Activation of Innate Immune Pathways and Restriction of HIV-1
library_construction_protocol: Monocytes were isolated from the peripheral blood of healthy donors by Ficoll-Paque density centrifugation followed by immunomagnetic selection using CD14+ microbeads (MACS monocyte isolation kit, Miltenyi Biotec) according to manufacturer's instructions.  MDMs were treated with anti-CCL2 or control Ab for 4 h and then infected with 3,000 tissue culture infectious dose (TCID50) per well of the CCR5-dependent HIV-1BaL strain pelleted virus (Advanced Biotechnologies), corresponding to a MOI ≈ 0.03. After 2 h, cells were washed and maintained in complete medium either in the presence or in the absence of anti-CCL2 or control Ab  Total RNA samples were isolated with the RNeasy Mini kit (Qiagen) following the manufacturer's instructions. RNA concentration and integrity were analyzed by a NanoDrop 2000 (Thermo Scientific) and a TapeStation (Agilent).   Total RNA was subjected to poly (A) selection followed by reverse transcription
identifiers.namespace: UCLA Institute for Quantitative and Computational Biosciences, University of California at Los Angeles, Los Angeles, CA, USA
identifiers.id: E-MTAB-9414:s10_s
attributes.tag: Experimental Factor: infect
attributes.value: none
attributes.tag: Experimental Factor: stimulus
attributes.value: none
attributes.tag: Experimental Factor: time
attributes.value: 4
attributes.tag: Experimental Factor: individual
attributes.value: donor4