alias: GSM3272768
title: GSM3272768: Damage-seq_Lung_2; Mus musculus; OTHER
library_construction_protocol:  Genomic DNA were extracted using PurLink Genomic DNA kit (Thermo).   Purified DNA (~1 µg) were used for End-repair and dA-tailing and adaptor ligation (NEBNext Ultra II DNA Library Prep Kit) following manufacture's instruction.  The DNA was then processed second adaptor ligation.   After quality check, the DNA samples were purified with HighPrep PCR beads, and ligated DNA were PCR amplified by NEBNext Ultra II PCR Master Mix with NEBNext Multiplex Oligos for Illumina (New England Biolabs).  The PCR libraries were purified with HighPrep PCR beads.  Libraries from all samples were pooled and sequenced using an Illumina HiSeq 4000 with 50-bp single-end read.
identifiers.namespace: GEO
identifiers.id: GSM3272768
attributes.tag: GEO Accession
attributes.value: GSM3272768