alias: OCI-20
title: Metataxonomic  of Milk from Ewe
design: A dual-barcoded 2-step PCR reaction was conducted to amplify a fragment of the V3V4 hypervariable region of the bacterial 16S ribosomal RNA (rRNA) gene. Equimolar concentrations of the universal primers S-D- Bact-0341-b-S-17 (ACACTGACGACATGGTTCTACACCTACGGGNGGCWGCAG) and S-D-Bact-0785-a-A-21 (TACGGTAGCAGAGACTTGGTCTGACTACHVGGGTATCTAATCC) were used as previously described (Klindworth et al., 2013).
identifiers.namespace: SUB5096463
identifiers.id: OCI-20