title: GSM3290702: ShML3-3-A4h; Homo sapiens; RNA-Seq
library_construction_protocol: For mRNA half life and translation efficiency, total RNA samples were isolated with Trizol following the manufacturer's instructions. For m6A MeRIP-Seq, portions of fresh tumor tissue approximately 0.5 x 0.5 x 0.5cm were snap frozen and preserved at -80C, pulverized in liquid nitrogen and stabilized in Trizol for total RNA isolation. Then the mRNA purification from total RNA was performed using PolyATtract mRNA Isolation Systems (Promega). 2g of the purified mRNA was fragmented and immunoprecipitated with α-m6A antibody (Synaptic Systems, 202003). The purified RNA fragments from m6A MeRIP were used for library construction using the TruSeq Stranded mRNA Sample Prep Kits (Illumina RS-122-2101) and sequenced with Illumina NextSeq 500. Libraries were prepared according to Illumina's instructions accompanying the TrueSeq Stranded mRNA library preparation kit.
attributes.tag: GEO Accession
attributes.value: GSM3290702