title: GSM3290404: 12: PQ NSC; Mus musculus; RNA-Seq
library_construction_protocol: TRIZOL 1) Total RNA treatment  a) RNase H Probe (rRNA depletion): Hybridize the rRNA with DNA probe and digest the DNA/RNA  hybrid strand, followed by DNase I reaction to remove DNA probe. Then obtain the target RNA after  purification.  b) Oligo dT Selection (mRNA enrichment): Use oligo dT beads to select mRNA with poly A tail.  2) RNA fragment and reverse transcription  Fragment the RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random primer.  3) End repair and adaptor ligation  End repair the dscDNA to blunt end and phosphate at 5' end, 3' end forms an “A” cohesive end. And  then ligate to the bubble adapter with protruding T of 3' end.  4) PCR amplification  5) Single strand separation and cyclization  Denature the PCR product by heat and the single strand DNA is cyclized by splint oligo and DNA  ligase.  6)DNA nanoball making7) Sequencing on BGISEQ-500 platform
attributes.tag: GEO Accession
attributes.value: GSM3290404