title: 16S_microbiome_Kidney_Mullus surmuletus_Yafo
design: Partial sequences of the 16S rRNA gene at the V4 hypervariable region were amplified using the following primers (Illumina Tags are in bold text): CS1-515F: ACACTGACGACATGGTTCTACA-GTGCCAGCMGCCGCGGTAA, CS2-806R: TACGGTAGCAGAGACTTGGTCT-GGACTACHVGGGTWTCTAAT. The PCR conditions were as follows: denaturation at 94C for 15 s, annealing at 50C for 20 s and extension at 72C for 30 s for 10 cycles with an additional 15 cycles with annealing at 60 C for 20 s. There were 25 cycles in total. PCR products were sent to HyLabs laboratories (Rehovot, Israel) for Next Generation Sequencing. Samples were checked by Qubit and Agilent TapeStation Tapestation, and loaded on the Illumina Miseq, using the V2-500 cycle kit, sequencing for 2x250 bp, which generated paired-end reads. The data was demultiplexed by Base-Space, and the FASTQ files were imported to CLC-bio platform.