title: 050A shotgun metagenome
design: Small sections of each sponge ground with 2ml sterile artificial seawater (ASW) with a sterile mortar and pestle. The resultant homogenate was centrifuged at 16000 rpm for 1 min to pellet the sponge cells. Genomic DNA (gDNA) was extracted using the ZR Fungal/Bacterial DNA MiniPrep kit (D6005, Zymo Research). Metagenomic sequencing was performed for the TIC2016-050A sample on the Ion S5 530 Chip and the other three samples using the Ion Torrent P1 platform (P1.1.17 chip).