title: GSM4226846: CDX2-Line2-ATAC; Mus musculus; ATAC-seq
library_construction_protocol: Orgnaoids were removed from matrigel using cell recovery solution and digested to single cells using Trypsin. Fresh viable single cells were isolated using flow sorting. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina). DNA libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs) and 150bp paired end sequencing was performed on the HiSeq-X (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4226846