title: GSM4873504: org_fetal_rep2_cage; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from established fetal and adult organoid cultures using Qiagen® RNEasy MicroKit. CAGE libraries for three biological replicates of fetal spheroids and adult organoids were prepared as previously described (Takahashi et al., 2012, Nat Protoc, doi:10.1038/nprot.2012.005) with an input of 3000 ng of total RNA. After individually preparing CAGE libraries for each sample, three samples with unique barcodes (CTT, GAT, and ACG) were pooled per sequencing lane and 30% Phi-X was spiked-in to each lane. Sequencing was done with Illumina HiSeq2000.
attributes.tag: GEO Accession
attributes.value: GSM4873504