title: HiSeq X Ten paired end sequencing; Gut microbiota and gallstone formation - cohousing study
design: Gut microbiota and gallstone formation - cohousing study
library_construction_protocol: Growth conditions were as follows: 1 AKR mice single housing fed with LD diet; 2 AKR mice cohousing fed with LD diet; 3 C57BL/6J mice cohousing fed with LD diet; 4 C57BL/6J mice single housing fed with LD diet. The protocol for co-housing experiment are as followed: AKR/J mice were given cocktail of antibiotics (containing 0.5 g/L vancomycin, 1 g/L neomycin sulfate, 1 g/L metronidazole, 1 g/L ampicillin) in drinking water&nbsp;ad libitumfor 3 weeks after weening (at the age of 4 week) to deplete gut microbes. Afterwards, the AKR/J mice were co-housing with conventional C57BL/6J mice at a ratio of 1:1 for 1-week and then fed with LD for 8 weeks' Liver tissues were harvested when the mice were sacrificed. RNA was prepared by Trizol reagent according to the protocol described. cDNA library generation with the TruSeqRNA Sample Preparation Kit (Illumina, San Diego, CA, USA). Clusters were generated with the TruSeq SR Cluster Kit v2 according to the reagent preparation guide.
attributes.tag: Experimental Factor: growth condition
attributes.value: C57BL/6 mice in single housing