title: RNA-seq of Cyprinodon: juvenile head
design: Total RNA was extracted from dissected heads of full-sib F1 lab reared embryos, larvae, or juvenile C. variegatus produced by breeding a single wild caught male and wild caught female. Heads were stored in RNA-later prior to RNA extraction. RNA was extracted using an ambion TRIzol Plus RNA Purification Kit. Illumina libraries were prepared for sequencing using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina with NEBNext poly(A) magnetic beads for mRNA isolation. RNA was chemically fragmented following the NEB protocol, except that the time was reduced to produce insert sizes of approx. 250bp. Random primers were used to produce cDNA.