title: RNA-Seq of Platythyrea punctata: old dominant, head: pp_101
design: Examination of gene expression patterns in dominant and subordinate workers of the clonal ant, Platythyrea punctata (different ages examined, head and abdomen tissue of dominants and subordinates). Workers were flash frozen in liquid nitrogen and stored at -80C until total RNA extraction. The heads and the abdomens of the ants were separated on ice and directly transferred into tubes filled with 10 l BL/TG-Buffer and homogenised with a pestle. Then, 240 l BL/TG-Buffer was added. Total RNA was extracted from the heads and abdomens separately using the ReliaPrepTM RNA Cell Miniprep System (Promega #Z6011), following the manufactors protocol. cDNA libraries were constructed with the transcriptome TruSeq RNA Library Prep Kit v2 (Illumina) and the SuperScript II Reverse Transcriptase (Thermo Fisher Scientific).