title: High-throughput microsatellite isolation for Lumbricus castaneus
design: A stoichiometric mixture of 9 DNAs was performed to produce a microsatellite enriched genomic library which was constructed by coupling multiplex microsatellite enrichment isolation techniques with the 454 GS-FLX Titanium pyrosequencing. Enrichment was performed using probes containing the following microsatellite motifs: TG, TC, AAC, AAG, AGG, ACG, ACAT and ACTC.