title: Illumina HiSeq 2000 sequencing; Reprogramming of the wheat transcriptome in response to infection with Claviceps purpurea, the causal agent of ergot
design: Reprogramming of the wheat transcriptome in response to infection with Claviceps purpurea, the causal agent of ergot
library_construction_protocol: Female flowers were examined at specific time points after Cp inoculation to follow the infection process through the stigma, the ovary transmitting tissue, to the ovule. Plants were grown in the glasshouse at an18oC/16 hour day and 15oC/8 hour night cycle. The middle florets of each ear were inoculated with a single C. purpurea isolate when the stigma became receptive (i.e. fluffy in appearance) Trizol (Invitrogen) method Illumina TruSeq library preparation
attributes.tag: Experimental Factor: time
attributes.value: 5
attributes.tag: Experimental Factor: sampling site
attributes.value: portion of transmitting tissue
attributes.tag: Experimental Factor: infect
attributes.value: none