title: GSM1217727: Toluene Males; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was extracted from 10 Drosophila images with ZR RNA MiniPrep Research; Irvine, CA, USA) per the manufacturer's instructions. The RNA quantity was determined using a Qubit® 2.0 Fluorometer (Invitrogen; NY, USA) and the RNA integrity (RNA Integrity Score ≥ 8) was determined using an Agilent 2100 Bioanalyzer (Agilent; Palo  Alto, CA, USA) per each manufacturer’s instructions. To prepare samples for the mRNA sequencing libraries, we used the Illumina TruSeq Sample Preparation Kit (Low-Throughput protocol) (Illumina 2012).
attributes.tag: GEO Accession
attributes.value: GSM1217727