title: Illumina NovaSeq 6000 paired end sequencing; RNA-Seq of cultivated (i) bulk human thymic epithelial cells (hTECs) (ii) cortical hTECs (iii) medullary hTECs (iv) human thymic interstitial cells (hTICs)
design: RNA-Seq of cultivated (i) bulk human thymic epithelial cells (hTECs) (ii) cortical hTECs (iii) medullary hTECs (iv) human thymic interstitial cells (hTICs)
library_construction_protocol: detachment of cells from 2d dish, freezing of cultivated cells into RNAlater. TECs: growth onto irradiated 3T3-J2 feeder layer. TICs: cultuvation in MegaCell medium in 2d dish. RNeasy Plus Micro Kit, Qiagen. TruSeqÂ® Stranded Total RNA.
attributes.tag: Experimental Factor: cell type
attributes.value: cortical thymic epithelial cell
attributes.tag: Experimental Factor: growth condition
attributes.value: cells cultivated onto 3T3-J2 feeeder layer