title: RNA-seq of Streptomyces coelicolor:  pelleting strain
design: RNA was extracted from mid-log phase cultures from YT2x medium, follwing a modified Kirby method. Total nucleic acid was precipitated and treated with DNAse I (Ambion, #AM2222). rRNA was depleted using the kit RiboZero rRNA Removal kit (Gram-Positive, #MRZGP126). Libraries were prepared following the protocol High Sample (HS) from the guide TruSeq Stranded mRNA Sample Preparation Guide (Illumina, 15031047 E).