title: GSM5342437: D1WT [RRBS]; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated from frozen brown adipose tissues Genomic DNA from brown adipose tissues was isolated by phenol chloroform extraction and a commercial service for DNA methylation sequencing was provided by Beijing Genomics Institute (BGI) (Shenzhen, China). Equal amount of genomic DNAs from 6 animals/group were pooled and used for RRBS analysis. According to the brochure provided by the company, the genomic DNA was digested with the methylation-insensitive restriction enzyme MspI and ligated to methylated sequencing adaptors. The ligated MSPI fragments were size-selected, treated with sodium bisulfite, amplified by PCR and constructed for library, which was sequenced.
attributes.tag: GEO Accession
attributes.value: GSM5342437