title: RNA-seq of maize: 5 mm tassel
design: 10  g polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads and fragmented at 95  C for 1 min, followed by end repair and 5' adaptor ligation. Reverse transcription was then performed with a RT primer harboring a 3' adaptor sequence and randomized hexamer. The cDNA was purified and amplified, and PCR products corresponding to 200-500 bp were purified quantified, and subjected to paired-end sequencing on the Illumina HiSeqTM 2000 system.