title: RNAseq of Senecio  spp (young plants)
design: Total RNA extacted  and treated with DNase, mRNA was selected and converted to cDNA. Second  strand cDNA synthesis to incorporate dUTP. The cDNA was end-repaired,  A-tailed and adapter-ligated. Prior to amplification, samples underwent  uridine digestion. The prepared libraries were size selected, multiplexed and  QCed before paired end sequencing.