title: GSM5170170: BS17-wt-HSV-HI; Mus musculus; RNA-Seq
library_construction_protocol: Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche).
attributes.tag: GEO Accession
attributes.value: GSM5170170