title: exosomal miRNA of PC-1.0 pancreatic cancer cell
design: Samples of PC-1.0 exosomes and PC-1 exosomes were prepared for high-throughput sequencing. In brief, total RNA of each sample was extracted using TRIzol  Reagent (invitrogen) and then 2 libraries were constructed using smRNA-seq(for illumina) (clontech, CA, USA) according to the manufacturer's instruction. RNA-seq was performed using illumina HiSeqTM 2500(Illumina, Inc., San Diego, CA, USA). Raw data was processed to identified differentially expressed miRNAs(DEMs). DEM data was assigned for further bioinformatic analysis.