title: NextSeq 500 paired end sequencing; RNA-Seq of canine transmissible venereal tumors
design: RNA-Seq of canine transmissible venereal tumors
library_construction_protocol: Biopsies of CTVT-5 and CTVT-6 were collected from dogs undergoing diagnostic assessment at the Department of Surgery and Obstetrics and Gynaecology, Faculty of Veterinary Medicine, University of Messina, Italy.  All the procedures on animals treated in Italy, including housing, conformed to EU regulations of animal welfare and are approved by the Italian Ministry of Health being in agreement with the directive D.P.C.M. of 28th February 2003. Nucleic acids were extracted from the biopsies using Qiagen RNAeasy Blood & Tissue kit following the manufacturer's instructions.  250 ng of total RNA was prepared for sequencing following the Illumina TruSeq mRNA (unstranded) protocol with insert size 150-250
attributes.tag: Experimental Factor: stationary
attributes.value: tumor grading
attributes.tag: Experimental Factor: CTVT6
attributes.value: individual