title: Soil 16S rRNA gene(V3-V4) gene  metabarcode: 1.5-year old post coal mining rehab site (Dorsfontein), replicate 5
design: The hypervariable V4 region of 16S rRNA library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage amplicon PCR step involving 0.2 M concentrations of primers 515F (forward) and 806R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter and Trimmomatic software