title: TA01-7G
design: Cotton seedlings with root-tip cuts at the two-true-leaf stage were used to inoculate 20 mL of V. dahliae spore suspension with 1  107 spores mL-1, and water was used as mock treatment. Roots 0 h, 24 h, 48 h, and 72 h after inoculation treatment were taken,  RNA  was extracted using the CTABacidic phenolic method. Pair-end high-throughput sequencing was performed on the Illumina HiSeq 2000 sequencing platform, and the sequencing raw reads were all 150 bp in length.