title: GSM5342484: HFD-RD-AT-Macro1; Mus musculus; ATAC-seq
library_construction_protocol: FACS-sorted ATMs were used for ATAC-seq and nuclei were isolated as previously described (Curr Protoc Mol Biol. 2016; 109: 21.29.1–21.29.9). Briefly, isolated cells were centrifuged at 500xg for 5 min at 4°C and then resuspended in ice-cold PBS+0.04% BSA. The cell lysis was performed for 5 minutes on ice by adding 45μl lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% Nonidet P-40, 0.001% Digitonin, and 1% BSA). After lysis, 50μl ice-cold wash buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, and 1% BSA) were added and then centrifuged at 500 × g for 5 min at 4 °C. After one more wash, the washed nuclei were counted and used for the transposase reaction. The library was constructed as cited previously (doi: 10.1002/0471142727.mb2129s109),   using Nextera Tn5 Transposase from Illumina
attributes.tag: GEO Accession
attributes.value: GSM5342484