title: root metagenome: Sample R_T_SL_SB_2
design: "Frozen root samples were pre-ground with mortar and pestles in liquid nitrogen.  DNA was extracted using the FastDNA SPIN Kit for Soil (MP BIO Biomedicals, Santa Ana, CA). Aliquots of DNA extracts were shipped overnight to the IGSB Next Generation Sequencing Core at Argonne National Laboratory for PCR amplification and sequencing.  Primers 515F and 806R targeting the V4 region of the 16S rRNA gene in Bacteria and Archaea were adopted from the Earth Microbiom Project (Caporaso et al. 2012)."