title: Genomic PCR of CRISPR edited  exon 2 of zfp36l1 in  U2OS TREx monoclonal cell line
design: U2OS-TREx cells were cultured in DMEM supplemented with 10% fetal bovine serum (Tet Minus FBS; ) and 1% penicillin/streptomycin (Invitrogen). Genomic DNA from CRISPR/Cas9-targeted ZFP36L1 clones was extracted using Qiagen QiaAmp DNA mini kit (#51304) according to the manufacturers instructions and was used as a template for PCR amplification. Forward (5-CTGCTGGACAGAAAGGCAGT-3) and reverse (5-ATCCACAACGCTGAAGAGCGC-3) primers spanning the CRISPR-edited region were used for amplification of a 450bp fragment of Exon2 of zfp36l1