title: ChIP-Seq of S. pneumoniae Mock: Rif 1xMIC
design: TruSeq ChIP Sample Prep Kit from Illumina was used following manufacturers instructions. Basically, DNA fragments of 150250 bp were selected after repairing ends, adenylating 3 ends, and ligating indexed paired-end adapters. The selected DNA fragments were enriched by PCR with the PCR primer cocktail that anneals to the ends of the adapters. Quality control of the library was determined with a 2100 Bioanalyzer Instrument (Agilent Technologies)