title: GSM2101543: rps16b-Null YPAD RNA-Seq rep2, sigma1278b deletion collection background; Saccharomyces cerevisiae Sigma1278b; RNA-Seq
library_construction_protocol: Cells were lysed by vortexing with glass beads in lysis buffer. Lysates were clarified by centrifugation at 16k xg. RNAse digestion of lysate and isolation of ribosomal footprints (Ribo-Seq) or random fragmentation and size selection (26-40 nt) of polyA-selected total RNA (RNA-Seq) was followed by 3' adapter ligation, reverse transcription, circularization of the RT product and PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM2101543