title: RNA-Seq of D. stibiiarsenatis MLFW-2: Quality trimmed and filtered reads for AsV_1F
design: RNA was extracted using a ZR Fungal/Bacterial RNA MiniPrep Kit (Zymo Research).  Genomic DNA was removed from the RNA extract using a Turbo DNA-free Kit (Thermo Fisher Scientific).  The 16S and 23S rRNA was depleted using a Ribo-zero rRNA Removal Kit (Illumina).  RNA fragmentation, first- and second-strand cDNA synthesis, A-tailing, adapter ligation, and library amplification was performed using KAPA Stranded RNA-Seq Library Preparation Kit (Kapa Biosystems).  Sequencing was performed on the Illumina MiSeq platform using a MiSeq Reagent Kit v3 (Illumina).