title: GSM4403039: H3K27me3_ChIPseq_KO_2; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIPseq, cells were fixed with 1% formaldehyde and insoluble chromatin fraction were isolated. Chromatin were sonicated to 200 to 500 base pair and protein-DNA complexes were pulled down by antibodies. For ChIPseq samples, libraries were prepared using ThruPLEX DNA-seq kit (R400428) according to manufacturer's instruction. Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing.
attributes.tag: GEO Accession
attributes.value: GSM4403039