title: GSM4874083: 24EMF2_RNA [RNA-seq]; Gallus gallus; RNA-Seq
library_construction_protocol: Both gDNA and RNA were extracted using Qiagen AllPrep Mini kit. RNA-seq libraries with barcode indexes were synthesized using ABI SOLiD 5500 Fragment Library Core Kit and subjected to 50-nucleotide, single-read multiplex sequencing with ABI SOLiD 5500XL deep sequencers. Genomic DNA was fragmented to ~200 bp by sonication at 4 °C using the Covaris S2 sonicator and subjected to enrichment of methylated DNA fragments using the MethylMiner kit (Life Technologies). Deep sequencing libraries were synthesized using ABI SOLiD 5500 Fragment Library Core Kit and subjected to 50-nucleotide, single-read sequencing with ABI SOLiD 5500XL deep sequencers.
attributes.tag: GEO Accession
attributes.value: GSM4874083