title: upf1-5_RNAseq_23degree_2
design: For transcriptome analysis, about one hundred 6-day-old seedlings of upf1-5 grown at 23C or 27C in growth chambers (GR-36, Percival Scientific, Canada) in SD were harvested around 1.00 PM (5 hours after the beginning of the light regime). The RNA was extracted using Isolate II RNA plant kit (Bioline Pty Ltd, Australia) and RNASeq was done with Illumina HiSeq2000 platform by BGI (BGI, China).