title: GSM5182762: BMDM_IgG_3 [159_09]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using RNeasy plus mini (Qiagen) following manufacturer's instructions. Isolated RNA sample quality was assessed by HS Total RNA 15nt on Fragment Analyzer (Agilent Technologies Inc., California, USA) and quantified by Qubit 2.0 RNA HS assay (ThermoFisher, Massachusetts, USA). Library construction is performed based on manufacturer's recommendation for SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input (Takara Bio USA Inc., California, USA). Final library size was about 300bp with an insert size of about 180bp Sequencing protocol: Illumina® 8-nt unique dual-indices were used. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina NovaSeq 6000(Illumina, California, USA) with a read length configuration of 150 Paired-End targeting150 M PE sequences per sample (75M in each direction).
attributes.tag: GEO Accession
attributes.value: GSM5182762