title: GSM2199533: ko Rep1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen)  following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol.
attributes.tag: GEO Accession
attributes.value: GSM2199533