title: WSCA_3_Archaea
design: Rumen fluid and solid contents was collected from 2 ruminally fistulated Holstein cows. The rumen inoculum was a mixture of rumen fluid and artificial saliva at a 8:5 ratio. Continuous culture with the rumen stimulation technique (RUSITEC) was performed using 650 ml of rumen innoculum, 70 g of rumen solid contents and 12 g of formula feed. After 120 h of acclimation period, 5.1 g of cellulose or 2.55 g of cellulose and 2.55 g of water-soluble cellulose acetate were added to formula feed individually, as control group or treatment group. Samples were collected 24 h after start of the test period and used for microbial analysis. Culture was performed in quadruplicate.