title: Amplicon sequence of peripheral blood mononuclear cells : healthy donor
design: PBMC from healthy donors were isolated and lysed in TRIzol directly, total RNA was extracted and reverse transcribed into cDNA,A set of forward primers, eachspecifictooneorasetoffunctionalTCRV-betasegments,anduniversalreverse primersspecifictotheconstantregionofTCRB,wereusedtogenerateampliconsthat covered the entire CDR14 region.