title: 16S rRNA human vaginal microbitoa
design: Dual indexed universal primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC) were used for PCR amplification of the V3-V4 hypervariable regions of the 16S rRNA gene, as previously described in Shipitsyna et al 2013;8(4) PloseOne and Fadrosh et al 2014;2(1):6 Microbiome