title: Pool of amplicons from five plates of the sugarcane community-based culture collection (CBC)
design: A community-based culture collection was constructed by picking colonies from primary platings that may contain single or multiple microorganisms, obtained from sugarcane organs. A strategy of 16S gene multiplexing was developed using two-step PCR amplifications with tagged primers for plates, rows and columns, allowing us to identify the composition of each obtained colony regardless if they contain single or multiple microorganisms. This method provides a high-throughput strategy for identifying isolated microbes in culture collection and allows fast and accurate cross-referencing with culture-independent community profiling. This dataset contains reads from five plates that were used to the development of these methodologies.