title: GSM3064694: HEL-iMKL1 Dox treated cells input rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were first fixed in 1% formaldehyde with shaking for 10 minutes at room temperature. After PBS wash, they were sonicated at 4degrees Celsius in a BioRuptor, to obtain 200-300bp fragment sizes. They were pre-cleaned by incubating with Millipore Protein A Salmon-Sperm DNA coated beads, with shaking for 1 hour at 4degrees Celsius. Fixed chromatin was then incubated overnight with either anti-SRF or anti-IgG antibody, followed by 1-hour incubation with coated beads the following morning. DNA was eluted from the beads using high to low salt solutions, with the final elute in freshly made Tris-EDTA (TE) buffer. The DNA was then treated with Proteinase K and reverse cross-linked with 5M NaCl, before being sent for sequencing. Single end DNA libraries were prepared for sequencing using standard Illumina protocols at the Yale Stem Cell Center Genomics Core
attributes.tag: GEO Accession
attributes.value: GSM3064694