title: RNA-seq homo sapiens: primary urothelial cancer : Sample M1502425
design: Formalin fixed parafin embeded tumor sample evaluated for quality and quantity using the Agilent RNA pico chip. Sample was prepared for sequencing by constructing an Illumina Tru-Seq Stranded RNA kit, according to the manufacturer's protocol. The resulting library was amplified by PCR, quantitated, pooled, and processed through a hybrid capture intermediate using the IDT xGen Exome reagent (as above). The captured fragments were quantitated, diluted, and were sequenced using 2 x 150 bp paired-end reads on the Illumina HiSeq 4000.
attributes.tag: alignment_software
attributes.value: STAR (v. 2.4.1d)