title: RNAseq of Senecio spp (young plants)
design: Total RNA extacted and treated with  DNase, mRNA was selected and converted to cDNA. Second strand cDNA synthesis  to incorporate dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated.  Prior to amplification, samples underwent uridine digestion. The prepared  libraries were size selected, multiplexed and QCed before paired end  sequencing.