title: Rna-seq of Poly-A+ mRNA from C. elegans strain NJ683, exc-7(rh252) : whole animal, L4 larval stage
design: C. elegans worms were synchronized by bleaching and grown to larval stage L4 under standard laboratory culturing conditions, NGM media with OP50 food source. Total RNA was extracted by Trizol as described in PMID:19778439 and PMID:24910101. poly-A RNA was purified and Library preparation was performed by the Illumina Fastback Genetic Analysis Services in Hayward, CA.