title: GSM3455647: RNA-seq_Bcat_Cerebellum_GFPneg_R1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from  from GFP-positive and GFP-negative FACS sorted cells using the RNeasy Micro Plus kit (74034, QIAGEN, Venlo, Netherlands). For RNA-seq, total RNA was converted to cDNA and amplified (Ovation Ultralow System V2-32, 0342HV, NuGEN Technologies, San Carlos, CA). Amplified cDNA was fragmented, end-repaired, linker-adapted, and single-end sequenced for 75 cycles on a NextSeq500 (Illumina Inc.).
attributes.tag: GEO Accession
attributes.value: GSM3455647