title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of four human glioblastoma cell lines from heterogeneous U-343 cell system
design: RNA-seq of four human glioblastoma cell lines from heterogeneous U-343 cell system
library_construction_protocol: Cells are seed in the plate until 80% confluence. Then cells were washed by PBS twice, and lysed with the lysis buffer. Cells were maintained in Minimum Essential Medium (MEM) with10% FBS and penicillin-streptomycin at 37°C with 5% CO2 and over 95% humidify.  Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific) Illumina TruSeq strand-specific RNA libraries (poly-A selection)
attributes.tag: Experimental Factor: cell line
attributes.value: U-343 MGa 31L