title: Roche SeqCap capture of human osteosarcoma: PATFVE Tumor
design: "The SeqCap EZ Target Enrichment System (Roche Sequencing, formally NimbleGen) was used to design the validation exome baits, which targeted exonic regions of 1807 genes selected based the discovery set results.  The total size of the targeted regions is 6,952,074 bp, and the total size of the bait regions is 8,473,358 bp with 98.8% targeted bases covered by the design. Library products made using Nextera DNA Sample Preparation Kits were captured using a Roche SeqCap Custom Capture kit (Roche) targeting a subset of exons of the human Exome (see bed file), per manufacturer specifications and protocols. Libraries L051016_TARGET-40-PATFVE-01A-01D and L051016_TARGET-40-NAAGJY-01A-01D can be differentiated from one another by parsing spots by read group."
attributes.tag: alignment_software
attributes.value: hg19