title: total RNA: EBOV-Minigenome transfected HEK293 cells
design: Poly(A)+ RNA was isolated from the total RNA samples. First-strand cDNA synthesis was primed using a N6 randomized primer. After fragmentation, the Illumina TruSeq sequencing adapters were ligated in a strand specific manner to the 5' and 3' ends of the cDNA fragments. The cDNA was finally amplified with PCR using a proof reading enzyme. The random-primed cDNA pool was size fractionated in the size range of 300 - 500 bp using a preparative agarose gel.