title: GSM4718633: MRC5_6_R2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted at 0, 6, 24, 48, 72 and 96 hours post-HCoV-229E infection, using NucliSENS® easyMag®, according to the manufacturer's protocol. At each time point, three biological repeats were tested. All RNA samples were treated with RQ1 RNase-free DNaseI (Promega, USA), according to the manufacturer's protocol. RNA quality was assessed using the Agilent High Sensitivity RNA ScreenTape for TapeStation System (Agilent Technologies, Germany). RNA samples (>8 RNA integrity number (RIN)) were prepared using the Illumina TruSeq RNA Library Preparation Kit v2 and sequenced in Illumina HiSeq 2500 Sequencer.
attributes.tag: GEO Accession
attributes.value: GSM4718633