title: RNAseq of mus musculus: primary microglia with or without osteopontin
design: Primary mouse microglia were treated with or without osteopontin for 24 h in culture. Total RNA was then isolated from cells using the Qiagen RNeasy kit with DNAse treatment for sequencing. Library was preppedby directional RNA library prep, poly A enriched and sequenced  on one NextSeq 75 cycle high output run.