title: Seasonal dynamics of the honey bee gut microbiota in colonies under subtropical climate
design: To further identify the changes in the composition and abundance of different genera belonging to the gut microbiota, DNA from 3 colonies at 6 sampling points (October and December 2014; February, April, June and August 2015) was analyzed through high throughput sequencing. The V4 region of 16S rRNA gene was amplified using primers Hyb515F_rRNA and Hyb806R_rRNA ([27], Supplementary data, Table 1) and sequenced using an Illumina MiSeq platform and 250 paired end (PE) cycles at the Genomic Sequencing and Analysis Facility, University of Texas at Austin, USA.