title: GSM3357426: ChIPseq_Wildtype_Input_DNA; Danio rerio; ChIP-Seq
library_construction_protocol: Embryos of 24 hpf were fixed, deyolked, and homogenized using pestles and sonication to isolate the chromatin In the regular non-spike in experiment zebrafish chromatin was incubated with antibody overnight. For spike-in experiments zebrafish chromatin was mixed with Drosophila spike-in chromatin (Active Motif 53083) and incubated overnight with H2Aγ (Active Motif 61686) and specific antibodies. After o/n antibody incubation the chromatin was extensive washed. The resulting ChIP-DNA was used as input for KAPA-HYPERprep library preparation.
attributes.tag: GEO Accession
attributes.value: GSM3357426