title: GSM3061429: revitalized MSCs RNA-Seq rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from 1,000-5,000 sorted MSCs was extracted using the RNeasy Plus Micro kit (QIAGEN), and assessed for integrity and purity using an Agilent 2100 Bioanalyzer (Agilent Technologies). Complementary DNA libraries were then generated using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) and the Nextera XT DNA Sample preparation Kit (Illumina). The libraries were then submitted for Illumina NextSeq 500 sequencing (150 bp single ended) according to the standard procedures (ENSEMBL Mus musculus reference genome GRCm38 release 77).
attributes.tag: GEO Accession
attributes.value: GSM3061429