title: Bemisia tabaci: single bacteriocyte transcriptome
design: The single cell samples were collected in tubes with lysis component and ribonuclease inhibitor. Then we carried out the amplification by the Smart Seq2 method. Then the cDNA production was checked by Qubit  3.0 Flurometer and Agilent 2100 Bioanalyzer. Then the cDNA was shearedrandomly by ultrasonic waves for Illumina library preparation protocol including DNAfragmentation, end repair, 3 ends A tailing, adapter ligation, PCR amplification andlibrary validation.