title: RNA-seq of shoot apex of a mutant line, pfr 59 of Corchorus olitorius variety, JRO-204 under short-day condition
design: Total RNA was extracted from the shoot apices of 60 day-old three individuals of a mutant line (pfr 59) of C. olitorius variety, JRO-204, devoid of any flower buds. The sample was derived under the short-day conditions during the month of November' 2015. RNA samples from the replicates of mutant line pfr 59 was pooled into one sample for RNA-seq library preparation using Illumina TruSeq Stranded Total RNA Library Preparation Kit. Prior to library preparation, the rRNA was depleted from the sample. The library was sequenced using 2x150 Paired-end (PE) chemistry and the raw reads were processed for de novo transcriptome assembly using Trinity. Differentially expressed transcripts between JRO-204 and its mutant's shoot apex was identified using the edgeR tool in Trinity.