title: Viral metagenomics of water samples from river ports along the Yangtze River in China:WaterControl
design: The diversity of viruses in the Yangtze River remains largely unexplored. Toinvestigatethefreshwater viromefrom the Yangtze River, totally 30 L water samples were collected from six river ports along the Yangtze River in 2017. Meanwhile, a control group of 5 L ddH2O was established. Eachsamplewascondensed into 2 mLusing the virus adsorption-elution method. The concentrated water samples were treated at 37 C with a mixture of DNases and RNase todigestunprotectednucleicacidfor60min.Remainingtotalnucleicacidwasthenisolatedusing QIAampMinEluteVirusSpinKitaccordingtomanufacturerprotocol.Sixlibrarieswerethenconstructed usingNexteraXTDNASamplePreparationKitandsequencedusingtheIlluminaMiSeq platform  with250basespairedendswithdualbarcodingforeachlibrary.Forbioinformaticsanalysis, pairedendreadsof250bpgeneratedby IlluminaMiSeqplatform  weredebarcodedusingvendorsoftwarefromIllumina.