title: TCR RNA-seq repertoire of mus musculus: adult Rras2-/- male splenic CD4 T cells
design: Splenic CD4 T cells were purified by negative selection. RNA was extracted using the RNAeasy Plus Mini Kit (QIAGEN). To perform the cDNA TCR library it was used the SMARTer Mouse TCR a/b Profiling Kit (Takara) following the manufacturers instructions. The kit incorporates the specific oligos to amplify the variable TCRa and TCRb regions adding the tags necessary for Illumina sequencing.