title: GSM5170704: RNAseq G1297 GFP EWS/ATF1 ON 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted by using the NucleoSpin RNA Plus (Takara Bio). RNA was quantified on a NanoDrop 2000c and Qubit. RNA (200 ng) was prepared for library construction. High-quality RNA (RNA Integrity Number value ≥8, as determined by Bioanalyzer) was used for library preparation. RNA-seq libraries were generated using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB). The number of PCR cycles was minimized to avoid skewing the representation of the libraries. RNA-seq libraries were sequenced on a NextSeq500 (Illumina) as 86 bp single reads.
attributes.tag: GEO Accession
attributes.value: GSM5170704