title: WGS surface water microbiome: site 3 wet
design: Concentrations of genomic dsDNA were measured using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) on an Invitrogen Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). DNA libraries were prepared using NextEra XT DNA Library Prep Kit (Illumina Inc., San Diego, CA, USA). All DNA libraries were quantified as previously mentioned and sequenced using an Illumina HiSeq4000 Instrument (Illumina Inc., San Diego, CA, USA) with a 2 x 150 bp run.