title: RNAseq of tetraploid watermelon
design: Total DNA and RNA were coextracted from 50 mg of watermelon leaves at seedling stage by using OMEGA Bio-Tek DNA/RNA isolation kit with some modifications. DNA and RNA were quality assessed in Nanodrop and gel electrophoresis, and the concentrations were measured by using Qubit RNA Broad Range and DNA High Sensitivity assays (Life Technologies). Purified RNA was spiked with External RNA Controls Consortium (ERCC) Spike-in Mix 1 according to the DNA/RNA ratio. Strand-specific RNAseq libraries were constructed from spiked RNA by using the NEBNext Poly (A) mRNA Magnetic Isolation Module and the NEBNext Ultra Directional RNA library kit for Illumina. Libraries were paired-end sequenced on an Illumina NextSeq 500 system with 75-bp read length.