title: GSM5342952: mammary tumor 264; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized and tumors were excised and cut in half, with one half flash frozen in liquid nitrogen. Flash-frozen tumors were later pulverized and total RNA was extracted using the Qiagen AllPrep kit (80004). RNA quality, quantity, and purity (260/280 absorbance ratios) were assessed using a Fragment Analyzer, a Qubit fluorometer, and a Nanoquant plate using an Infinite 200 PRO absorbance reader, respectively. Total RNA was diluted with nuclease free water to a normalized concentration (33.33 ng/uL) and sequenced.
attributes.tag: GEO Accession
attributes.value: GSM5342952