title: ITS library of coastal seawater - 06 Sep 2011 (weekly sample)
design: We extracted DNA using phenol-chloroform chemical extraction (Massana et al., 1997) supplemented with three rounds of bead beating (30 seconds each). The fungal internal transcribed spacer (ITS) region was amplified using the dual index PCR primers ITS1-F 5 -CTTGGTCATTTAGAGGAAGTAA-3  and ITS4 5 -TCCTCCGCTTATTGATATGC-3  (Bruns & Gardes, 1993)