title: GSM4306930: LEC BALB/c_Oxa-48h; Mus musculus; RNA-Seq
library_construction_protocol: Axillary, inguinal and brachial LNs from 25-30 male and female BALB/cJ or C57BL/6J mice were combined, minced, washed with Hanks' Balanced Salt solution, and dissociated for 30 min at 37 °C with gentle rocking in HBSS (with calcium and magnesium) medium containing 0.2 mg/ml Collagenase P, 0.8 mg/ml Dispase II and 0.01 mg/ml DNase I (Sigma-Aldrich). Hematopoietic cells were depleted with anti-CD45 mouse MicroBeads according to the manufacture's protocol (Miltenyi) and stained with anti-CD31 BV605 (clone 390) and anti-Podoplanin PE-Cy7 (clone 8.1.1) antibodies, as well as dump antibodies consisting of anti-CD45 (clone 30-F11), anti-EpCAM (clone G8.8), anti-TER119 (clone TER-119), anti-CD11a (clone H155-78) and anti-CD11b (clone M1/70). Total EC (lin- CD31+) were sorted into 100% fetal bovine serum using FACS Aria III (BD Biosciences; 100 um nozzle; ~2500 cells/second) and washed. scRNA-seq libraries were generated using the Chromium Single Cell 3' Library and Gel Bead Kit v2 (10x Genomics) according to manufacturer's instructions.
attributes.tag: GEO Accession
attributes.value: GSM4306930