title: RNA-seq of c.elegans: CX12311
design: CX12311, PTM66, PTM88, LSJ2 L4 hermaphrodites were picked to fresh NGM agar plates. Their adult progeny were bleached using alkaline-bleach solution to isolate eggs for synchronization. The eggs were washed with M9 buffer for three times and placed on a tube roller overnight. About 400 hatched L1 animals were placed on NGM agar plates and incubated at 20C until they reach young adulthood. Young adulthood was defined as the timepoint when eggs were observed on assay plates. These worms were then harvested and washed for 3 times with M9 buffer. Samples were then kept frozen in -80 oC freezer until RNA extraction. Each strain and condition had 1 independent replicates on 3 different days.