title: STD_offspring
design: STD_offspring_01 .=> TCACGTACTA STD_offspring_02 .=> CGTCTAGTAC STD_offspring_03 .=> TCTACGTAGC STD_offspring_04 .=> TGTACTACTC STD_offspring_05 .=> ACGACTACAG STD_offspring_06 .=> CGTAGACTAG STD_offspring_07 .=> TACGAGTATG STD_offspring_08 .=> TACTCTCGTG STD_offspring_09 .=> TAGAGACGAG STD_offspring_10 .=> TCGTCGCTCG STD_offspring_11 .=> ACATACGCGT STD_offspring_12 .=> ACGCGAGTAT STD_offspring_13 .=> ACTACTATGT STD_offspring_14 .=> ACTGTACAGT STD_offspring_15 .=> AGACTATACT STD_offspring_16 .=> AGCGTCGTCT  The amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche).