title: GSM5261318: KH2 mESC CONTROL 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation from de-differentiated KH2 mouse embryonic stem cells (mESCs) was done using a picopure RNA isolation kit (Thermo-Fisher Scientific, Waltham, USA). Samples were tested on the Agilent 2100 Bioanalyzer to determine RNA integrity and quantity. The NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The sample  preparation was performed according to the protocol "NEBNext Ultra Directional RNA Library Prep Kit for  Illumina" (NEB #E7420S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After  fragmentation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing  adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation  was measured with the Fragment Analyzer (see appendices). The size of the resulting products was  consistent with the expected size distribution (a broad peak between 300-500 bp). Clustering and DNA sequencing using the Illumina NextSeq 500 was performed according to manufacturer's  protocols. A concentration of 1.6 pM of DNA was used. NextSeq control software 2.0.2 was used.
attributes.tag: GEO Accession
attributes.value: GSM5261318