title: GSM4874025: Arab-4G-R4-1; root metagenome; OTHER
library_construction_protocol: Psph strains were grown, harvested and washed as above, then the bacteria were transferred to MM at 28 ℃ for 6 hours. Total RNA was extracted by using RNeasy Mini Kit (QIAGEN, USA). Genomic DNA was removed by using DNaseI (NEB, USA) for 30 min. After removing rRNA by using the MICROBExpress kit (Ambion), mRNA was used to generate the cDNA library according to the NEBNext UltraTM II RNA Library Prep Kit protocol (NEB, USA), which was then sequenced using the HiSeq 2000 system (Illumina, USA). All RNA-seq samples have two biological replications. NEBNext UltraTM II RNA Library Prep Kit protocol (NEB, USA)
attributes.tag: GEO Accession
attributes.value: GSM4874025