title: Metagenomic sequencing of methane seep sediment from Santa Monica Mounds
design: DNA from biomass samples (~2mL) was extracted using the FastDNA SPIN kit for soil (MPBio) according to the manufacturer s protocol.  DNA concentrations were quantified using the Quant-iT dsDNA HS assay kit (Invitrogen) as per the manufacturer s instructions. The paired-end library was prepared using the Nextera XT DNA library preparation kit (Illumina, USA) for DNA extracted. The libraries were sequenced on a NextSeq500 (Illumina, USA) platform generating 2 150 bp paired-end reads with an average insert length of 300 bp.