title: RNA-seq Bra 9_5
design: Mature male and female C57BL/6 mice were mated overnight, the vaginal plug detected on the following morning, and the time of the presence of a vaginal plug designated as E0.5. NTD mouse embryos were induced by gavage with 28mg/kg (body weight) of all-trans retinoic acid (ATRA) (Sigma, USA) (dissolved in oil) on E7.5. On E9.5, pregnant mice were euthanized by cervical dislocation and embryos were dissected from decidual tissue and placed in ice-cold, DEPC-treated PBS. Nine mouse embryos collected from three independent pools, brain vesicle tissue were collected.