title: Illumina MiSeq sequencing of SAMD00065466
library_construction_protocol: The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values.