title: STD_mothers
design: STD_mother_01=>TACTGAGCTA STD_mother_02=>ACGCTCGACA STD_mother_03=>AGACGCACTC  The amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche).