title: Illumina HiSeq 4000 paired end sequencing; ATAC-seq in GR18 cell line comparing cells treated for 90 minutes with 1 micro molar dexamethasone with vehicle (ethanol) control-treated cells
design: ATAC-seq in GR18 cell line comparing cells treated for 90 minutes with 1 micro molar dexamethasone with vehicle (ethanol) control-treated cells
library_construction_protocol: ATAC-seq was performed with 100,000 cells per treatment, cells were grown in DMEM contining 5% fetal bovine serum. GR18 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 90 minutes with 1uM Dexamethasone or 0.1% ethanol as vehicle control ATAC-seq was performed with 100,000 cells per treatment according to the Omni-ATAC-seq protocol [PMID: 28846090]. DNA was extracted using Zymo Columns (DNA Clean & Concentrator Kit, Zymo research #D4014) Library amplification involved PCR 11 cycles using the NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, Cat #M0541).
attributes.tag: Experimental Factor: compound
attributes.value: dexamethasone
attributes.tag: Experimental Factor: dose
attributes.value: 1