title: Illumina MiSeq paired end sequencing
design: Generation of a complex metagenomic dataset from porcine faecal material.
library_construction_protocol: DNA extracts were prepared from 440mg of porcine faeces. Selective lysis was performed using the MolYsis selective lysis kit (Molzym), followed by DNase treatment and nucleic acid extraction using the MasterPure complete kit (Epicentre). Libraries were generated using Nextera XT library preparation kit (Illumina).