title: McMurdo Dry Valley Lakes metagenome; archaea
attributes.tag: nucl_acid_amp
attributes.value: DOI: 10.1126/science.114668
attributes.tag: pcr_primers
attributes.value: 958FWD:AATTGGANTCAACGCCGG;1048REV-major:CGRCGGCCATGCACCWC;1048REV-minor:CGRCRGCCATGYACCWC
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V6
attributes.tag: nucl_acid_ext
attributes.value: bead beating alkaline lysis
attributes.tag: pcr_cond
attributes.value: initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles