title: GSM4769440: EV_miRNA_uninfected_24hpi_1; Homo sapiens; miRNA-Seq
library_construction_protocol: For the analysis of intracellular miRNAs, total RNA was extracted from infected and uninfected lt-NES® cells at 24, 48 and 72 hpi using miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer's instructions. For the analysis of EV-derived miRNAs, cell culture supernatants were collected at the indicated time points and EV-associated miRNAs were extracted using exoRNeasy Serum/Plasma Kit (Qiagen, 77064) as described by the manufacturer. RNA sequencing libraries were generated using the NEBNext Mulitplex Small RNA Library Prep Set for Illumina (NEB E7300S) following size selection by running the samples on a 10 % polyacrylamide gel and isolating the 147 bp. Samples were purified using the QIAquick PCR Purification Kit (Qiagen, 28104). Libraries were validated on a High Sensitivity DNA chip using the 2100 Bioanalyzer (Agilent).
attributes.tag: GEO Accession
attributes.value: GSM4769440