title: GSM4769326: Sorghum leaves-control degradome; Sorghum bicolor; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from leaves of control, drought or heat, drought and heat-exposed sorghum using the Trizol reagent Degradome libraries were constructed using poly-A+ RNA, which was ligated to a 5'adapter containing a MmeI site at its 3'-end. The ligated products were used for cDNA production and amplified by PCR for five cycles. The PCR products were digested with MmeI and the resulting fragments ligated to a second double-stranded oligonucleotide, purified and amplified for another ten PCR cycles. The PCR product has been purified and sequenced.
attributes.tag: GEO Accession
attributes.value: GSM4769326