title: GSM4769121: ChIP-Seq Input Wild type#2; Mus musculus; ChIP-Seq
library_construction_protocol: islets were isolated after collagenase digestion and density gradient purification and then hand picked for purity, flash frozen in liquid nitrogen. For Chip-seq they were fixed with formaldehyde and then after sonication (300-500bp), IP was performed on 10 micrograms of chromatin with Anti-Tead1 antibody or the IgG isotype control. For ATAC-seq 100,000 of the frozen nuclei  were used. Chip-Seq - Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). ATAC-seq - 100,000 nuclei were tagmented as previously described (Buenrostro et al. 2013), with some modifications based on (Corces et al. 2017) using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter). Chip-seq and ATAC-seq
attributes.tag: GEO Accession
attributes.value: GSM4769121