title: GSM4306939: AGD1289 replicate 2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted by standard Trizol extraction techniques. Libraries were made using either NEBNext mRNA Second Strand Synthesis Module (E6111) followed by NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB-E7645) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NE7490) as per manufacturers protocols using half reactions. 13 Cycles of amplification was used for library enrichment; quality and size distribution of the the libraries was ascertained by running on a Bioanalyzer High Sensitivity DNA Chip (Agilent 5067-4626) and concentration was determined using KAPA Library Quantification Kit (KK4824). Libraries were sequenced on an Illumina HiSeq 2500 system by the Babraham Sequencing Facility.
attributes.tag: GEO Accession
attributes.value: GSM4306939