title: Chloroplast complete genome  of Leymus chinensis:LC_cp
design: After the DNA samples were tested to be qualified, they were randomly interrupted by Covaris ultrasonic crusher, and then the whole library was prepared by terminal repair, adding a tail, adding sequencing connector, purification, PCR amplification and other steps. After the construction of the library, Qubit 2.0 was used for preliminary quantification, the library was diluted, and then Agilent 2100 was used to detect the inserted fragments of the library. After the size of the inserted fragments met the expectation, Q-PCR method was used to accurately quantify the effective concentration of the library to ensure the quality of the library.