title: GSM4874328: ATAC_NICD_rep3; Mus musculus; ATAC-seq
library_construction_protocol: 50'000 CLL cells were isolated from sorted spenocytes (CD19+B220-) and processed as in Current Protocol in Molecular Biology by Buenrostro et al. in 2015 ATAC-Seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Library was amplify using NEBNext High-Fidelity 2X PCR Master Mix and size selection was performed using Agencourt AMPure XP beads. The primer list can be found in Current Protocol in Molecular Biology by Buenrostro et al. in 2015 ATAC-Seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Prepared libraries were sequenced on the Illumina NextSeq 500 with paired-end 2x79nt reads.
attributes.tag: GEO Accession
attributes.value: GSM4874328