title: GSM4769518: SNCA; Mus musculus; RNA-Seq
library_construction_protocol: The brain nigral regions were carefully dissected from 2 subsequent 500μm-thick slices and  the tissue directly subjected for RNA extraction or processed for the single cell dissociation. The  dissociation was performed using Adult Brain Dissociation Kit and OctoMACS Separator (both  from Miltenyi Biotec) following manufacturer's instructions. This procedure collected a large  fraction of different cell populations ranging from immune cells to neurons within the same  preparation. Cells were stained with 1:200 Zombie Aqua Fixable Viability Kit (Biolegend) and  subsequently with anti-CD45 PerCP-Cy5.5 antibody and anti-CD11b eFluor450 antibody (all  from eBioscience). Cells were sorted using a FACSAria Fusion (BD) and collected in PBS 0.04%  BSA to be processed for sequencing through the Chromium platform (10X Genomics). Cells  were separated into droplet emulsion using the Chromium Single Cell 3' Solution (V3.0) and  Single-cell RNA-seq libraries were prepared according to the Single Cell 3' Reagent Kits User  Guide (V3.0). Libraries were sequenced on a Novaseq 6000 flowcell (Illumina), with a minimum  depth of 50K reads/cell.
attributes.tag: GEO Accession
attributes.value: GSM4769518