title: GSM3724684: RG_21nt_6w_repB; Mus musculus; miRNA-Seq
library_construction_protocol: Liver samples were snap frozen in liquid nitrogen and ground into powder.  Samples were homogenized and RNA was extracted by Trizol 3ug of RNA for liver samples and 1ug of alll other samples were ligated to Linker-1 (IDT) in the absence of ATP with T4 RNA ligase 1 (New England Biolabs). Ligated RNA was size selected (based on an 18-36nt small RNA fraction) on 15% PAGE. The 5' ends RNA were ligated to 4nt barcoded adapters. Dual-ligated RNA was reverse transcribed, PCR amplified and size selected on 4% NuSieve (Lonza) agarose.  Small RNA sequencing was performed on the Illumina miSeq platform.
attributes.tag: GEO Accession
attributes.value: GSM3724684