title: GSM3060997: C13: BDE-47 exposed; Mus musculus; RNA-Seq
library_construction_protocol: Gonadal fat from the right side of each animal was collected, weighed and snap frozen in liquid nitrogen and stored at -80°C. Total RNA was isolated using TRIzol reagent (Invitrogen). The NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England BioLabs Inc., Ipswich, MA) was then used to isolate intact poly(A)+ RNA from 3 µg of total RNA. Libraries were constructed using NEBNext mRNA Library Prep Reagent Set for Illumina with multiplexing indexes from NEBNext Multiplex Oligos for Illumina (E6100 and E7335 respectively, New England BioLabs Inc., Ipswich, MA). High-throughput sequencing was performed on a NextSeq500 sequencing system (Illumina, San-Diego, CA) in the Genomic Resource Laboratory of the University of Massachusetts, Amherst. cDNA libraries were single-end sequenced in 75 cycles using NextSeq 500 High Output Kit (FC-404-1005, Illumina, San-Diego, CA) in a multiplex run.
attributes.tag: GEO Accession
attributes.value: GSM3060997