title: RNA-Seq of Ricinus communis: Leaves control 2
design: Total RNA was isolated from 100 mg of the sample, consisting  of a mixture of three plants. Two biological replicas were prepared, REP1 and REP2. RNA was extracted with the Rneasy Plant Mini Kit, and > 10 ug were used for each sample. The mRNA was separated with poly-dT beads, then fragmented to an average size of 200 bp and used to prepare cDNA, with random primers to start the first-strand synthesis. cDNA ends were repaired and 3'A was added before ligation of adapters with indexes. A strand-specific protocol was followed. After pre-amplification by PCR, the process of cluster generation and sequencing followed.