title: GUIDE-seq in U2OS.EGFP cell line: Wt Cas9 with HEK site 4 sgRNA
design: GUIDE-seq; tag-specific primers that anneal to either the (P)ositive or (N)egative strand of the integrated dsODN were used to generate two sets of 'single-tail' PCR amplicons, each subjected to 2 x 150 paired-end sequencing.