title: GSM3357297: G10_Lancaster_d45_rep7; Homo sapiens; RNA-Seq
library_construction_protocol: To isolate RNA of single organoids we used the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer's instructions. We assessed RNA concentration and purity using a NanoDrop 8000 spectrophotometer and RNA integrity with a Bioanalyzer (Agilent Technologies) per standard protocols. mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation) und quantified using the NEBNext Library Quant Kit for Illumina (NEB).
attributes.tag: GEO Accession
attributes.value: GSM3357297