title: GSM2628441: WT CD25 Low rep 2; Mus musculus; RNA-Seq
library_construction_protocol: T regs were isolated from splenocyes and lymph nodes of Atg5+/+ DC and Atg5-/- DC mice by CD4+ enrichment and FACS sorting to isolate CD4+ CD62L+ CD25 High or CD4+ CD62L+ CD25 Low  populations.  Total RNA was extracted using RNeasy Mini Kit. SMARTer™ Ultra Low RNA kit (Clontech) was used for the reverse transcription and cDNA amplification. 10 ng of total RNA was used as input for library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2628441