title: WGS of Leishmania donovani: 1S2D promastigote P125
design: promastigotes sequenced after 125 passeges in culture. 0.3-0.6 micrograms of sheared genomic DNA (Covaris E201) was end-repaired, adenylated and ligated to Illumina specific indexed paired-end adapters (adapter:insert molar ratio 40:1). The DNA library was size selected with AMPure XP beads in order to reach an insert size of 220-550bp and to remove unligated adapters. The final library was quantified with the Library Quantification Kit (Kapa Biosystems). On the base of the qPCR quantification the molar concentration sufficient for sequencing run was estimated. The library was sequenced using TruSeq SBS Kit v3-HS (Illumina Inc.) in paired end mode in a fraction of one sequencing lane of an HiSeq2000 flowcell v3 (Illumina Inc.) according to standard Illumina operation procedures