title: Illumina MiSeq of environmental fungi: Castanea rhizosphere
design: Ribosomal RNA gene Internal Transcribed Spacer (ITS2) amplicons (barcode+primer combination: TTAACCTTCCTG+GTGARTCATCGAATCTTTG and AGATTGACCAAC+TCCTCCGCTTATTGATATGC) sequenced after NEBNext adapter/index addition using MiSeq Reagent Kit v3.