title: RNA-Seq of arctic ground squirrel: adult male skeletal muscle
design: Poly-A RNA was isolated from total RNA and chemically fragmented. First and second strand synthesis was followed by end repair, and adenosines was added to the 3' ends. Adapters were ligated to the cDNA and 200  25 bp fragments were gel purified and enriched by PCR. Then the cDNA was run on the Illumina HiSeq 4000 Sequencer.