title: GSM5261817: P1DMSO6: Passage 1 DMSO-treated endothelial cells 6; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed using Buffer RLT (Qiagen) supplemented with 1% beta-mercaptoethanol. RNA was isolated using the RNeasy Plus Micro Kit (Qiagen) according to manufacturer instructions. First-strand cDNA synthesis (with polyA enrichment) was performed using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio), followed by 9 cycles of PCR amplification. Libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM5261817