title: Genomic PCR of exon 2 of zfp36l1 in Wildtype U2OS TREx cell line
design: U2OS-TREx cells were cultured in DMEM supplemented with 10% fetal bovine serum (Tet Minus FBS; PanBiotech) and 1% penicillin/streptomycin (Invitrogen). Genomic DNA from wild type cells was extracted using Qiagen QiaAmp DNA mini kit (#51304) according to the manufacturers instructions and was used as a template for PCR amplification. Forward (5-CTGCTGGACAGAAAGGCAGT-3) and reverse (5-ATCCACAACGCTGAAGAGCGC-3) primers spanning the CRISPR-edited region  for amplification of a 450bp fragment of Exon2 of zfp36l1