title: GSM4874360: Mut1_P46 Lepr-Cre;mTmG;Smo+/- (Rep 1); Mus musculus; RNA-Seq
library_construction_protocol: Dorsal skin from P46 Lepr-Cre;mTmG;Smo (n=2 technical replicates) and Lepr-Cre;mTmG;Smo+/- (n=2 technical replicates)  female mice were isolated and processed into single cells as previously described in Guerrero-Juarez, CF, et al. Nat Commun. 2019 with minor modifications. Dorsal skin from P50 Lepr-Cre;mTmG female mice were isolated and processed into single cells as previously described in Guerrero-Juarez, CF, et al. Nat Commun. 2019 with minor modifications. Viable, GFP-positive cells were physicially sorted via FACS. Single cells were captured using the Chromium Platform (10X Genomics) and cDNA libraries were generated using Single Cell 3' v2 (sorted GFP-positive cells) and v3 chemistries (unsorted, whole dorsal skin cells). Single cells were quantified using a hemocytometer. Cell capture, cDNA library preparation, quality control, and sequencing was performed at UC Irvine Genomic High Throughput Facility. Single Cell 3' v2 Chemistry - 10X Genomics
attributes.tag: GEO Accession
attributes.value: GSM4874360