title: GSM2101285: B6_brain_d5_PbA_3; Mus musculus; RNA-Seq
library_construction_protocol: Brains from perfused PbA-infected mice (at day 5) and from spinal cords of mice undergoing EAE (at day 7) were harvested and frozen in liquid nitrogen. Total RNA was isolated using Trizol-chloroform (Life Technologies), followed by DNase-digestion and purification on RNeasy columns (Qiagen). RNA integrity was assessed on a Bioanalyzer RNA pico chip. RNA-seq libraries were prepared following rRNA depletion and using Illumina TruSeq Stranded Total RNA Library preparation kit. The RNA-seq libraries were sequenced on an Illumina HiSeq 2500 sequencer in paired-end 50bp configuration.
attributes.tag: GEO Accession
attributes.value: GSM2101285