title: RNA-Seq of Duchenne muscular dystrophy iPSC-CMs: Male DMD
design: RNA-seq libraries were produced according to manufacture protocol (NEBNext Ultralldirectional RNA library prep kit for Illumina, cat. no. E7760) using 800 ng total RNA. mRNAs pull-down was performed using Magnetic Isolation Module (NEB, cat. no. E7490). All libraries weremixed into a single tube with equal molarity. The RNA-seq data was generated on IlluminaNextSeq500, 75 single-end read in high-output mode (Illumina, cat. no. 20024906).