title: GSM3061782: Tumor-plate3-36; Mus musculus; ATAC-seq
library_construction_protocol: single cell MI-ATAC of primary mouse breast cancer cells. Single cells were first fixed, then  lysed, followed by staining for respective antibodies and transposition. The transposition reaction is the same as for standard ATAC-seq except with 0.05% Igepal CA-630 in the lysis buffer. After 30 min transposition, at 37 degrees, the reaction was quenched using 40 mM EDTA, then cells were centrifuged and supernatant was discarded. 20 µl of reverse crosslinking solution was added to the cell pellet (with final concentration of 5 ng/ml proteinase K).  Reverse crosslinking was performed 65 degree overnight and inactivated by a 10 min incubation at 80 degrees the next day. The 25 µl PCR master mix (NEB, M0541S) and 5 µl of two unique primer combinations were directly added to the reverse crosslinking mixture and PCR was performed as previous described. The PCR product was purified with Qiagen MinElute purification kit and eluted in 20 µl Qiagen EB elution buffer
attributes.tag: GEO Accession
attributes.value: GSM3061782