title: GSM4396438: T09 [1 dpi]; Physcomitrella patens; RNA-Seq
library_construction_protocol: The total RNA of Mock plants, 0.5, 1, 2, and 3 dpi were extracted using TAKARA Trizol Reagent according to the protocol of manufacture. The total RNA was treated with RNase-free DNase I to eliminate DNA contamination.The enrichment of mRNA was performed with Oligo (dT)-attached magnetic beads,and fragmentation was performed using divalent cations under high temperature in NEBNext First Strand Synthesis Reaction Buffer (5×) to randomly interrupt mRNA. The first-strand cDNA chain was synthesized by random hexamers and M-MuLV Reverse Transcriptase (RNase H) with the mRNA template. Subsequently, the second-strand cDNA chain was synthesized by adding buffer, dNTPs, RNase H and DNA polymerase I. The the double stranded cDNA was purified using AMPure XP beads. Following end-pair and single nucleotide A (adenine) addition for the purified cDNA, adapters were used to distinguish the different samples by selection with AMPure XP beads for PCR amplification, following which the cDNA library was obtained.
attributes.tag: GEO Accession
attributes.value: GSM4396438