title: RNA sequencing of Erysiphe necator
design: Poly-A RNA was isolated, whole transcriptome amplified, and converted to cDNA with the Qiagen Quantitect Whole Transcriptome Kit.  cDNA was fragmented to 200-250 bp using Covaris, ends were repaired, and Illumina adapters T/A ligated, and PCR amplified prior to sequencing.  cDNA was sent to the Cornell University Sequencing facility in Ithaca, NY for sequencing library preparation and sequencing on an Illumina sequencer (Illumina, San Diego, CA, USA).