title: Sorted on CD155-high
design: DNA was extracted from 6x10*5 sorted CD155-high cells using the QIAamp DNA FFPE Tissue Kit (Qiagen). Individual integrated gRNA sequences were amplified by PCR (primers: sgRNA_outer_shortF: 5- GCTTACCGTAACTTGAAAGTATTTCG-3; KLV_sgRNA_outerR: 5-CGAGACTAGTGAGACGTGCTAC-3.Illumina adaptors were added in an additional round of PCR (P5_sgRNA_inner_F: 5-AATGATACGGCGACCACCGAGATCTACACTCTCTTGTGGAAAGGACGAAACACCG-3;P7_index_KLV_sgRNA_R: 5-CAAGCAGAAGACGGCATACGAGATNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT CCATTTGTCACGTCCTGCACG-3'Sequencing was carried out using the Illumina Miniseq platform, Illumina sequencing primer: 5-AGACTATAAGTATCCCTTGGAGAACCACCTTGTTGG-3.