title: GSM4769516: DATB; Mus musculus; RNA-Seq
library_construction_protocol: Fresh brain tissue was collected from saline-perfused animals. Once the brain was removed, the nigral tissue was manually dissected and lysated in Trizol for the RNA isolation. RNA libraries were generated starting from 1 microgram of total RNA whose quality was assessed by using a Tape Station instrument (Agilent). To avoid over-representation of 3' ends, only high-quality RNA with an RNA Integrity Number (RIN) ≥ 9 was used. RNA was processed according to either TruSeq Stranded mRNA Library Prep Kit. The libraries were sequenced on Illumina HiSeq 3000 with 76 bp stranded reads using Illumina TruSeq technology. Image processing and base calling were performed using the Illumina Real-Time Analysis Software.
attributes.tag: GEO Accession
attributes.value: GSM4769516