title: RNA-Seq of human flu: buccal swab
design: Sequencing using NEBNext RNA Sequencing Kit along with rRNA Depletion Kit (NEB,). Paired-end sequencing of multiple pooled samples was performed using MiSeq reagent kit v3 (Illumina). Sequence data were trimmed, assembled and mapped against the Genbank reference sequences using Geneious 11.0 software (Biomatters) with default parameters