title: bacterial 16S-rRNA-gene-seq of seawater metagenome: s23p250
design: DNA from filter sections was extracted using the UltraClean Soil DNA Isolation Kit (MoBio). Tag sequencing and prior PCR-amplification were performed at the Research and Testing Laboratory (Lubbock, Tx). Primers 28F (GAGTTTGATCNTGGCTCAG) and 519R (GTNTTACNGCGGCKGCTG) were used for amplification of the variable regions V1-3 for bacteria.