title: Illumina MiSeq paired end sequencing
design: Construction of a mixed viral metagenomic dataset using 25 DNA and RNA viruses
library_construction_protocol: The RNA library was prepared using a combination of the PureLink viral RNA/DNA kit (Life Technologies - Invitrogen) and Zymo RNA clean and concentrator kit, followed by turbo Dnase treatment (Life Technologies – Ambion). Reverse transcription and amplification was performed using the Ovation RNA-Seq version 2 system (Nugen). Libraries were generated using Nextera XT library preparation kit (Illumina).