title: Naive pool Read 1 line1
design: 10000 cells per sample were loaded in to the 10X Chromium controller and library preparation was performed according to the manufacturers indications (Chromium Next GEM Single Cell 3 Reagent Kits v3 protocol). The resulting libraries were sequenced in an Illumina NovaSeq6000 sequencer according to 10X Genomics recommendations (paired-end reads, R1=28, i7=8, R2=91) to a depth of around 50,000 reads per cell. 10x libraries were prepared and sequenced at the Functional Genomics Center (University of Zrich).