title: GSM3898336: WT_AngII; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from tissue was isolated from the left ventricle of one WT mouse with 28 days of Angiotensin II infusion. Single cell suspension was prepared from the adult left ventricle of one mice with Angiotensin II infusion for 28 days. After removal of dead cells with MACS dead cell removal kit (Miltenyi Biotec, #130-090-101), cells were lysed and subsequently RNA was reverse-transcribed and converted into cDNA libraries for RNA-seq analysis using a Chromium Controller and a Chromium Single Cell 3′ v2 Reagent kit (Genomics 10x) following the manufacturer's protocol. The library was sequenced using the Illumina Hi-Seq3000 sequencing platform.
attributes.tag: GEO Accession
attributes.value: GSM3898336