title: Illumina NovaSeq 6000 paired end sequencing; RNAseq of iPSC derived cardiomyocytes in response to  endothelin-1 as a model for cardiac hypertrophy
design: RNAseq of iPSC derived cardiomyocytes in response to  endothelin-1 as a model for cardiac hypertrophy
library_construction_protocol: The study was conducted with cardiomyocytes obtained from Takara Bio. The cardiomyocytes are derived from Human induced pluripotent stem cell line ChiPS 22, however, the differentiation protocol is proprietary information owned by Takara Bio. Cardiomyocytes derived from induced pluripotent  stem cells were exposed to endothelin-1, 10nM, for up to 96 hours. Total RNA was extracted using MagMAXâ„¢ Total RNA Isolation Kit (AM1830, Invitrogen, www.thermofisher.com) Library construction was performed using Illumina Truseq stranded total RNA with Illumina Ribozero method
attributes.tag: Experimental Factor: stimulus
attributes.value: endothelin-1, 10 nanomolar
attributes.tag: Experimental Factor: time
attributes.value: 96