title: circular RNA-seq of Megalobrama amblycephala
design: Ten M. amblycephala tissues were sampled, including brain, eyes, heart, liver, spleen, kidney, intestines, skin, muscle, gills. Following the manufacturer's instructions, TRIZOL reagent was used to extract total RNA of each sample, which was mixed equally after passing quality test. The circRNAs were enriched by ribosomal RNA removal and RNase R treatment.