title: Illumina 16S amplicon sequence of bacteria in maple seedling roots
design: internal transcribed region (ITS) amplified with ITS1F and ITS2 primerset. .  All amplified PCR samples were cleaned and normalized using the Invitrogen Sequalprep PCR Cleanup and Normalization Kit. DNA library was sequenced using an Illumina MiSeq Sequencer with the 2 300 bp paired end platform using the Illunima MiSeq Reagent Kit v3.