title: RNA-Seq of Homo Sapiens: Polyp2MAST_mRNA
design: Polyp2MAST_mRNA NS500531 sequenced on NextSeq500. Two arrays were sequenced per sequencing run with an Illumina 75 Cycle NextSeq500/550v2 kit (Illumina FC-404- 2005) at a final concentration of 2.2-2.8 pM. The read structure was paired end with read 1 starting from a custom read 1 primer12 containing 20 bases with a 12-bp cell barcode and 8-bp unique molecular identifier (UMI) and read 2 containing 50 bases of transcript information.