title: Multi-omics of human faecal sample: metagenomics
design: Libraries with an insert size of 350 bp were constructed from metagenomic DNA by fragmentation by sonification (Covaris), end-repair, adenylation, adapter ligation and amplifaction of adapter-ligated DNA fragments using appropriate enzymes (Enzymatics). Library amplification and cluster generation were performed using TruSeq PE Cluster Kit V3-cBot-HS (Illumina) and flow cells were sequenced with 101 bp per paired end on a HiSeq2000 system (Illumina).