title: Illumina HiSeq 2500 sequencing; Transcriptome analysis of Triticum monococcum in response to cold stress (4 degrees C for 24 hours)
design: Transcriptome analysis of Triticum monococcum in response to cold stress (4 degrees C for 24 hours)
library_construction_protocol: Plants were grown in normal greenhouse growth conditions  Control samples were maintained at normal conditions at 23 degrees Celsius for 24 hours. Cold samples were exposed to temperature of 4 degrees Celsius for 24 hours.  Total RNA was extracted from frozen plant tissue. Invitrogen Plant RNA Reagent, chloroform, isopropanol, and DNAse were used to obtain RNA. Qiagen RNA cleanup kit was used to remove any enzymes used in extraction.  Single-end libraries were quantified by qPCR and prepared using an Illumina TruSeq RNA prep kit
attributes.tag: Experimental Factor: organism
attributes.value: Triticum monococcum subsp. monococcum
attributes.tag: Experimental Factor: environmental stress
attributes.value: no stimulus
attributes.tag: Experimental Factor: strain
attributes.value: accession DV92