title: GSM3897744: RNAseq_WT_Rep2_Cl13_d8; Mus musculus; RNA-Seq
library_construction_protocol: To assess the transcriptional and epigenetic impact of TOX deletion in T cells, 250 WT and 250 TOX-/- naïve CD44LOWCD62LHI P14 cells sorted from peripheral blood of donors, mixed, and co-transferred into WT mice. Recipients were subsequently infected with LCMV Cl-13 and splenocytes were harvested 8 days following infection. Ten spleens were pooled for each of the 3 replicates prior to processing, CD8+ T cell enrichment (using EasySep CD8+ T cell negative selection kit, Stem Cell Technologies), and staining of single cell suspensions. 1x105 WT and TOX-/- P14 cells were sorted to a purity of >98% for each replicate. To extract RNA, 50,000 cells were resuspended in buffer RLT supplemented with beta-mercaptoethanol and processed with a RNeasy Micro Kit (Qiagen) as per the manufacturer's instructions. Total RNA libraries were prepared using a Pico Input SMARTer Stranded Total RNA-Seq Kit (Takara).