title: ChIP-seq of maize: 5 mm tassel
design: Approximately 1 g immature tassel (~5 mm) tissue was harvested from 35Spro:GIF1-GFP plants grown and immediately cross-linked in buffer containing 1% formaldehyde for 15 min under a vacuum, followed by the addition of glycine to a concentration of 0.1 M and infiltration for 5 min. After three washes with distilled water (4  C), the cross-linked tissues were dried with paper towels and flash frozen in liquid nitrogen. Chromatin extracted from 35Spro:GIF1-GFP plants was immunoprecipitated with anti-GFP antibody with a Plant ChIP-seq kit (Diagenode Inc., Denville, USA) according to the manufacturer s instructions.