title: RNA-seq of Henneguya salminicola
design: RNA was extracted from a single Henneguya salminicola cyst using the Roche High Pure RNA extraction kit, with on-column DNAse treatment, per the manufacturer's instructions. The samples were sent for a library construction and sequencing to the Center for Genome Research and Biocomputing (CGRB) of Oregon State University (Corvalis, OR). The RNA libraries were prepared with the Wafergen PrepX kits (PrepX PolyA mRNA Isolation Kit and PrepX RNA-Seq Library Prep). Paired-end sequencing of 150 bp reads derived from fragments of average length of ~320 bp was performed on a HiSeq3000 platform.