title: RNA-seq of c.elegans: PTM512
design: adult hermaphrodites were picked to fresh 6cm NGM agar plates and let lay eggs for 3 hours. Worms were then picked off and the eggs remained on plates were cultured at 20C until they reach L4 stage. Heat shock assay plates were then wrapped with parafilm and placed in a water bath pre-heated to 34C for 2 hours and 4 hours. Worms were either collected right after heat shock or 30 minutes later for the recovery group. Each strain and condition had 3 independent replicates on the same day.