title: McMurdo Dry Valley Lakes metagenome; bacteria
attributes.tag: nucl_acid_amp
attributes.value: DOI: 10.1126/science.1146689
attributes.tag: pcr_primers
attributes.value: 967FWD-PP:CNACGCGAAGAACCTTANC;967FWD-UC1:CAACGCGAAAAACCTTACC;967FWD-UC2:CAACGCGCAGAACCTTACC;967FWD-UC3 :ATACGCGARGAACCTTACC;967FWD-AQ :CTAACCGANGAACCTYACC;1046REV:CGACAGCCATGCANCACCT;1046REV-PP:CGACAACCATGCANCACCT;1046R-AQ1 :CGACGGCCATGCANCACCT;1046R-AQ2:CGACGACCATGCANCACCT
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V6
attributes.tag: nucl_acid_ext
attributes.value: bead beating alkaline lysis
attributes.tag: pcr_cond
attributes.value: initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles