title: GSM4873786: DETC_anti-Skint1_treated_scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Ears were split and floated upon Trypsin-GNK (2.5mg/ml trypsin (Sigma), 7.35mg/ml NaCl, 0.35mg/ml KCl, 0.85mg/ml glucose) for 1 hr at 37 degrees C. Epidermis was separated and digested in trypsin-GNK with 100 ug/ml DNase I (Roche) and 1X Dnase buffer (1.21 mg/ml Tris base, 0.5mg/ml MgCl2, 73ug/ml CaCl2) for 20 min at 37 degrees C with agitation. Isolated cells were washed in medium and stained with Live/Dead Fixable Near-IR Dye (Molecular Probes), followed by mouse Fc block (BD) and anti-CD45-APC (30-F11, BioLegend), anti-MHC-II I-A/I-E-FITC (M5/114.15.2, BioLegend) and anti-CD3-PE (2C11, BioLegend). Live CD45+CD3+ MHC-II- cells were sorted with a FACS Aria Fusion. RNA libraries were prepared for sequencing using Chromium Single Cell 3' GEM, Library and Gel Bead Kit v3 (10x Genomics)
attributes.tag: GEO Accession
attributes.value: GSM4873786