title: Aphidius ervi SA strain 8kbp Mate Pairs reads
design: DNA was extracted from adult haploid males of A. ervi in seven sub-samples (ca. 120 males each). Total DNA was extracted using the DNEasy Plant Mini Kit (QIAGEN, Hilden, Germany) following the manufacturers instructions). DNA was quantified by spectrophotometry (Epoch Microplate Spectrophotometer, Biotek) and fluorometry (Qubit 3.0; Qubit DNA High sensitivity Assay Kit, Invitrogen) and DNA quality was assessed on 1% agarose gel electrophoresis. DNA samples were sent in dry ice to MACROGEN (Seoul, South Korea). Extracted DNA was used to produce Illumina paired-end (PE) and mate-pair (MP) libraries for sequencing. 6 DNA samples (720 individuals) were pooled  and used for MP sequencing (3kb, 5kb and 8kb insert sizes), which were prepared with the Nextera mate-pair protocol (Illumina, San Diego, CA).