title: GSM4768561: GSC_KD_2; Homo sapiens; RNA-Seq
library_construction_protocol: The RNA was extracted using Qiagen RNeasy kit following the manufacturer's protocol.  The RNA concentration and quality was validated using Bioanalyzer. RNA was extracted using RNeasy kit (Qiagen).  For ribosomal depletion, 1–2 mg of total RNA was used for Ribo-Zero rRNA Removal kit (Illumina) according to the manufacturer's protocol. Ribosomal-depleted library was processed for TruSeq Stranded Total RNA sequencing according to the manufacturer's protocol (Illumina). The quality and quantity of total RNA and final libraries were assessed using Agilent Technologies 2100 Bioanalyzer.  RIN (RNA Integrity Number) of >9 was used for the sequencing experiments.  Libraries were sequenced on the Illumina HiSeq 4000 following the manufacturer's protocols.
attributes.tag: GEO Accession
attributes.value: GSM4768561