title: Mutational analysis of signet ring cell colorectal cancer using next generation sequencing
design: Next-generation sequencing was carried out according to manufacturer’s instructions on the Ion PGM™ System using Ion AmpliSeq Cancer Hotspot Panel v2 (Life Technologies, UK). 50 ng of DNA was used. Libraries were prepared using Ion AmpliSeq Library Kit 2.0 and quantified using the Agilent 2100 Bioanalyzer system (Agilent Technologies, UK). The Ion OneTouch 2 System was used to generate template-positive sphere particles for sequencing. Samples were run on Ion 318 chips (Life Technologies, UK).