title: GSM2974444: ALL54_Dex rep3_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Cell suspensions of spleens were prepared and mononuclear cells enriched to >97% human by density gradient centrifugation. The spleen-harvested cells were used for ChIP-seq assay. Lysates were clarified from sonicated nuclei and crosslinked protein-DNA complexes were isolated with antibody. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared by Novogene (Hong Kong) for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM2974444