title: GSM4874241: Dermatitis_tape_NL (41); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Exiqon miRCURY™ RNA Isolation Kit - Cell and Plant (Exiqon, now Qiagen Denmark, Copenhagen, Denmark) following the manufacturer's recommendation, with additional heating of the lysis buffer to 56°C for 5 min before adding 70% ethanol (Qiagen Denmark, Copenhagen, Denmark). Library preparation was performed with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara Bio Europe, Saint-Germain-en-Laye, France) following the manufacturer's recommendation without fragmentation. To capture all RNA transcripts, the kit uses random hexamer primers for amplification. The protocol included removal of ribosomal RNA. For comparability, the same protocol was used for all sample types. Single-end sequencing (75 bp) was performed with a NextSeq 500 (Illumina Denmark, Copenhagen, Denmark)
attributes.tag: GEO Accession
attributes.value: GSM4874241