title: Whole-genome of human parvovirus B19: Human blood
design: Whole-genome libraries were prepared using the NEB Ultra II kit (Cat. No: E7645L). In brief, the total DNA extracted from EDTA blood  was subjected to a sequence of enzymatic steps to fragment them, the ends of these fragments were repaired enzymatically and tailing with dA-tail was done followed by ligation of adapter sequences. The adapters ligated fragments were then cleaned up using Agencourt AMPure XP bead (Cat.No: A63882). Next, these fragments were indexed using limited cycle PCR to generate final libraries for paired-end sequencing.