title: Cloned sequences of Gram negative PAH-RHD gene: Reference Site 7 January 2011
design: DNA extracted from the soil sample was PCR amplified with the primer set described. Amplicons were gel purified and cloned with pCR 4-TOPO vectors. The vector DNA was digested and sequenced by Eurofins. The sequences were converted from ab1 to fastq, leading and trailing Ns were trimmed, and sequences shorter than 200 bp were discarded. The vector DNA is not removed from the sequences published in this file to allow for alignment and trimming to the target.