title: GSM686382: embryo_sphere_rep1; Danio rerio; ncRNA-Seq
library_construction_protocol: Zebrafish embryos were collected at the 256-cell stage, sphere stage, shield stage, and 1dpf.  Total RNA was isolated from embryos using Trizol.  RNAs were fractionated on 15% denaturing polyacrylamide gels and small RNAs between 15-30 nucleotides were excised and purified.  cDNA libraries were generated using specific linkers and RT/PCR, as previously described.  Libraries were sequenced in the Genome Technology Core of Vanderbilt University using the Illumina sequencing platform.
attributes.tag: GEO Accession
attributes.value: GSM686382