title: RNA-seq of Perionyx excavatus: Head60d
design: A library was independently prepared with 1 g of total RNA for each sample using an Illumina TruSeq Stranded Total RNA Sample Prep Kit (Illumina, Inc., San Diego, California). The rRNA in total RNA was depleted using a Ribo-Zero kit. After the rRNA was depleted, the remaining RNA was purified, fragmented, and primed for cDNA synthesis.