title: SOLiD fragment RNA-Seq of Effect of the Menstrual Cycle on Breast Epithelium DNA Expression sample S5
design: Normal breast epithelium of these 20 specimens was microdissected from multiple 8 micron frozen tissue sections.  Total RNA extracted from the tissue and was subsequently depleted of rRNA via locked nucleic acid probes (Ribominus, Invitrogen, Carlsbad, CA). Barcoded cDNA libraries from the 20 normal breast epithelia were prepared and sequenced on an Applied Biosystems (AB) SOLiD3 or SOLiD 4 platform. RNA-seq reads for each sample were then mapped to the human genome (hg19) using the LifeScope software version 2.5.1 (Life Technologies, Foster City, CA) and BAM (Binary Alignment/Map) files generated.