title: Illumina HiSeq 2000 paired end sequencing; RNA-seq of the Octopus vulgaris nervous system.
design: RNA-seq of the Octopus vulgaris nervous system.
library_construction_protocol: Three specimens of Octopus vulgaris (2 males, 1 female; body weight 300 g) were caught by local fishermen in the Bay of Napoli (Italy) during the summer 2012. Total RNA was isolated from the supra- (SEM) and sub-esophageal (SUB) masses, optic lobes (OL) and the arms (ARM) using SV total RNA isolation System (Promega, #Z3100) according to the manufacturerâs protocol. DNA was degraded by treating samples with Turbo DNase Kit (Ambion, #AM1907) according to the instruction manual. Quality and quantity of RNA was assessed by NanoDrop (Thermo-Fisher, #ND-2000) and RNA BioAnalyzer chip (Agilent Technologies, #5067-1513).  Paired-end libraries were prepared using Illumina TruSeq RNA sample library preparation kit (Illumina, # RS-930-2002).
attributes.tag: Experimental Factor: subesophageal mass
attributes.value: organism part