title: RNA-Seq of Arabidopsis thaliana: 20-day seedling (QQS RNAi)
design: RNA-Seq was determined from Arabidopsis T2 seedling shoots grown in soil in a growth chamber at 20 days after planting (DAP) at 22 degree under long day conditions (16 hours of light/8 hours of dark). For Arabidopsis RNA-Seq, total RNA from six shoots was extracted for each biological replicate and purified. Two biological replicates were used for each mutant line (QQS-OE, QQS-RNAi), and three were used for the WT controls. Both the 200-bp short-insert library and the transcriptome sequencing (an Illumina HiSeq 2000 system with V3 reagent, 91 pair-end sequencing) were conducted at BGI Americas (http://bgiamericas.com).