title: Investigating the grapevine endobiome
design: DNA was extracted from 100 mg of ground wood sHA-Fvings from both asymptomatic and symptomatic inner grapevine tissues with a CTAB extraction buffer and DNeasy Plant Mini DNA samples of 70ng were used for diversity profiling analysis. PCR amplification and sequencing was performed at the Australian Genome Research Facility (AGRF, Sydney, NSW, Australia), targeting the ITS region of the rRNA genes. Illumina indexing of the amplicons was achieved in a second PCR. Indexed amplicon libraries were quantified by fluorometry (Promega Quantifluor) and an equimolar pool was created and adjusted to 5 nM for sequencing on an Illumina MiSeq (San Diego, CA, USA) with a V3, 600 cycle kit (2 x 300 base pair, paired-end reads).