title: GSM4305164: FSbC-4: SOX9+ cells; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs from four independent sets of FSbC-expanded SOX9+ cells (passage 4 to 6, derived from H9 hESCs), and four independent sets of PNC/PN-treated GDF5+ cells were extracted with an RNeasy mini kit (Qiagen), followed by DNaseI treatment, and then RNA purification Poly (A)-tailed messenger RNA was enriched and the RNA-seq library was prepared in the UTHealth Cancer Genomics Core by following the instructions of KAPA mRNA HyperPrep Kit (KK8581, Roche Holding AG, Switzerland) and KAPA Unique Dual-indexed Adapter kit (KK8727, Roche).  RNA-seq was performed using the Illumina Nextseq550 system with the 75 bp pair-ended running mode
attributes.tag: GEO Accession
attributes.value: GSM4305164