title: soil bacteria of parasitism by Cuscuta australis
design: The V3-V4 region of the 16 S rDNA gene were amplified and  Illumine adapte were added.  Amplicon were cleaned up using AMPure XP beads, then attached dual indices and Illumina sequencing adapters using the Nextera XT Index KIT. The library was cleaned up by using AMPure XP beads and quantified by using a fluorometric quantification method on Agilent Technologies 2100 Bioanalyzer tracer. Libraries were normalized and up to 96 libraries were pooled for a Miseq run.