title: AMPLICON of Human Gut Metagenome: IBD patients intestinal tissue
design: DNA was amplified using bacterial 16S v4 rDNA fusion primers designed against surrounding conserved regions and tailed to incorporate Illumina adaptors and indexing barcodes. Amplified products were then purified, quantified by PicoGreen, then pooled to equimolar concentrations. Pooled libraries were loaded into a MiSeq reagent cartridge v2 and onto an Illumina MiSeq. After cluster formation, amplicons were sequenced for 250 cycles with custom primers designed for paired-end sequencing.