title: Amplicon seq, adenine base editing, SGCA c.157G>A mutation, off-target 1, human muscle stem cells
design: PCR amplicon off-target site 1, ZNF571 locus. Primers: fwd(5>3) GGTGCGGAGGAACACAACTA / rev(5>3) AGTCCCACAGTTCGAGCAAG. Q5 DNA Polymerase. Amplicon checked with 2100 Bioanalyzer using DNA 1000 Kit (Agilent).