title: Pyrosequencing analysis of bacterial diversity and community composition using 16S rRNA gene V1-V3 region as the target-CBS-LA55
design: "The total community DNA was extracted from soils by modified hot SDS-lysis method. Universal primers 27F and barcoded-518R were used for PCR amplification of the V1?V3 hypervariable regions of 16S rRNA genes. PCR products were quantified, purified and sequenced by Roche 454 GS-FLX Titanium for assessment of bacterial diversity and community structure. "