title: GSM2628179: WT1_RNASeq; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA from thirty dissected drosophila midguts per sample was prepared using TRIzol reagent (Invitrogen, Carlsbad, California, USA), and cleaned by following RNeasy kit (Qiagen) total RNA cleanup protocol. Libraries were prepared using NEBNext Adaptor and then size-selected 125 bp-150 bp paired-end reads were generated. Prior to sequencing the integrity and quality of cDNA libraries were assessed using an Agilent 2100 Bioanalyzer and an ABI StepOnePlus real-time PCR system.