title: GSM1847485: Smik_A; Saccharomyces mikatae; MNase-Seq
library_construction_protocol: Based on Pan et al. 2011 Cell 144:719-731. Samples were harvested at the indicated time-points after transfer to sporulation conditions, crosslinked with 1% formaldehyde, spheroplasted, chromatin extracted, treated with a dilution series of micrococcal nuclease (MNase) from 5 to 80 units, and DNA purified, as described in Pan et al. 2011. Mononucleosome-sized DNA was then purified by size fractionation on agarose gels and libraries prepared for Illumina sequencing.
attributes.tag: GEO Accession
attributes.value: GSM1847485