title: pKPSH169 Klebsiella pneumoniae plasmid isolated from Shafdan” Dan Region Wastewater Treatment and Reclamation Project site
design: Plasmids from the seven E. coli DH10β transformants were extracted as described above. Removal of chromosomal DNA was validated by PCR targeting of the 16S rRNA gene as previously described (Marilley et al., 1998). One ng of plasmid DNA from each transformant was fragmented, tagged with adapters, and libraries were prepared using the Nextera XT DNA Sample Preparation Kit according to the manufacturer's protocol (Nextera XT, Illumina Inc, San Diego 92122, CA, USA). Samples were then sequenced using the Illumina MiSeq platform (Illumina, San Diego, CA, USA).