title: GSM4989596: si-lncRNA-3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted, and the quality of the RNA was assessed with the Agilent Bioanalyzer 2100. Transcriptome libraries from the mRNA fractions were generated following the RNA-Seq protocol (Illumina). Each sample was sequenced in a single lane with the Illumina PE150 Analyzer (Illumina) on a 150-bp paired-end run.   For strand-specific library construction, dUTP method of second-strand marking was employed as described. For the data processing, the raw sequencing reads were aligned to human reference genome (hg19) using the splice-aware aligner HISAT2. Read counts for each gene were normalized into FPKM (Fragments Per Kilobase of transcript per Million mapped reads) values(66). The cutoff of differential gene expression was FDR <0.05, normalized by the respective control group. Transcriptome libraries from the mRNA fractions were generated following the RNA-Seq protocol (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4989596