title: Rice_P4B Transcriptome of P4 stage leaf from Oryza sativa indica IR64
design: Primordia were dissected and flash frozen in liquid nitrogen. RNA was extracted using Trizol reagent and cleaned up and DNase1 treated using the Sigma Plant Total RNA kit (Sigma Aldrich). RNA-Seq was carried out at the Liverpool Centre for Genomic Research (www.liv.ac.uk/genomic-research) using RiboZero treated RNA with library construction following the Illumina TruSeq stranded mRNA protocol (www.illumina.com). Sequencing (Illumina HiSeq 2000) produced paired end reads with a read length of 100 bp.