title: day_dentifera_6
design: The Qiagen DNEasy Blood & Tissue Kit was used on a sample of 5 Daphnia magna to extract DNA. Samples were taken midday or mid-night to illuminate differences in microbiome composition across circadian rhythms. Libraries were prepared using the 515f/806r universal 16S primer pair, amplifying the V4 region of the 16S rRNA gene. Samples were multiplexed for the Illumina MiSeq and run with the MiSeq v2 Reagent Kit.