title: GSM4306188: wt-pcontrol, H3K9me2 ChIP-seq; Schizosaccharomyces pombe; ChIP-Seq
library_construction_protocol: Cells were lysed by bead beating and chromatin was sheared by sonication. Lysates were incubated with antibody and Protein-G Dynabeads overnight. Immunoprecipitated genomic DNA was recovered using PCR purification columns. Libraries were prepared for Illumina sequencing with 1-5 ng of ChIP or 10 ng of input DNA using NEXTflex adaptors and Ampure XP beads. Barcoded libraries were pooled and sequenced following an Illumina MiniSeq workflow.
attributes.tag: GEO Accession
attributes.value: GSM4306188