title: RNA-Seq of mus musculus: adult male pancreatic islet
design: High throughput sequencing libraries were constructed from total RNA extracted from pancreatic islets of  mice maintained on HFD for ~10 weeks.  RNAs with RIN 8, as assessed by the Agilent 2100 Bioanalyzer system, were used to prepare transcriptome libraries using the NEBNext Ultra RNAlibrary prep kit (New England Biolabs).