title: GSM4815658: in vitro ligation-Rep3; Homo sapiens; OTHER
library_construction_protocol: Forty-eight hours after transfection, total RNAs were extracted by RNAiso Plus reagent (TaKaRa) and reverse transcripted into cDNAs by PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa). KOD DNA polymerase was used to amplify nanobody sequences. PCR products were subjected to sequencing library construction by using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4815658