title: GSM5342857: fatbody_M_RNA1_rep3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Drosophila melanogaster females were flash-frozen in liquid nitrogen and stored at -80oC. Females were then defrosted and the head, fat body (abdomen minus gut and ovaries) and ovaries were dissected out (over dry ice), pooled into groups of 11 or 12 within each treatment and sub-group, then flash-frozen in liquid nitrogen, and stored at -80oC. Each sample (tissue pooled from 11 or 12 individuals) was then ground in liquid nitrogen using a micropestle with the resulting powder resuspended in Tri-reagent (Sigma-Aldrich, UK). RNA was extracted using the Direct-zolTM RNA extraction kit (Zymo Research, USA) according to the manufacturer's protocol. An additional DNase treatment was performed using TurboTM DNA-free kit (Thermo Fisher Scientific, UK), according to the manufacturer's protocol. Illumina TruSeq stranded mRNA-seq libraries constructed by Edinburgh Genomics (Edinburgh, UK)
attributes.tag: GEO Accession
attributes.value: GSM5342857