title: GSM4306275: Cisplatin-4; Homo sapiens; RNA-Seq
library_construction_protocol: Kidney organoids were grown from MANZ-2-2 iPSCs according to Przepiorski et al., 2018. Quadruplicate samples of >100 organoids per sample were left untreated (= samples 1 – 4) or treated with 5 µM cisplatin (= samples 5 – 8) on day (d) 12, d14, d16 and d18. On d19, organoids were washed in PBS and homogenized in TRIzol. Total RNA was extracted using the GENEzol TriRNA Pure kit (Geneaid). Libraries were prepared using the Collibri™ Stranded RNA Library Kit (Thermo Fisher) and RNA-sequencing was carried out on an Illumina NextSeq 500. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4306275