title: GSM3061408: Bis_addbackWt_1; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was isolated using QIAamp DNA mini kit from Qiagen (cat. No 51304) and fragmented using Covaris S220 Focused-ultrasonicator. 500ng of fragmented DNA was then used for library preparation with NEBNext Ultra DNA Library Prep Kit for Illumina (NEB #E7370) and bisulfite treatment with EZ DNA-methylation Gold Kit (Zymo Research #D5005). Final PCR amplification was performed using KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (KAPA Biosystems #KR0413) and 12 cycles of amplification.
attributes.tag: GEO Accession
attributes.value: GSM3061408