title: RNA-seq of RNA extracted from distal colon stool samples of mus musculus mono-colonized with E.coli Nissle: Control mouse 4 (DC4)
design: RNA was extracted from stool samples with RNeasy PowerMicrobiome kit (Qiagen) following manufacturer's protocol, including on-column DNAse I treatement. Purified RNA was depleted of bacterial ribosomal RNA, and converted into barcoded cDNA before sequencing. rRNA depletion, library preparation and sequencing service provided by The Centre for Applied Genomics (TCAG), Toronto, Canada (http://www.tcag.ca/).