title: GSM4305657: HC 3; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood (10 mL) was obtained from each subject and all the samples were collected in the ethylene diamine tetraacetic acid (EDTA) tubes. Peripheral blood mononuclear cells (PBMCs) were isolated using the Ficoll density gradient centrifugation and were counted using Cellometer Auto T4 (Nexcelom Bioscience, USA).Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and the concentration of total RNA was measured by NanoDrop2000c spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM4305657