title: PpTPL2-N176H control rep1
design: Total RNA prepared from approximately 100mg wet weight tissue (protonemata, blotted dry) using the Qiagen RNeasy plant mini kit, with on-column DNase treatment. Oligo-dT-primed library was prepared using the TruSeq sample preparation kit (Illumina). All steps were performed in duplicate or triplicate to ensure independent biological replicates for RNA-seq.