title: Effects of paternal circadian disruption on offspring transcription
design: Total RNA was prepared from adrenal glands using the TRIzol reagent (Thermo Fisher) according to the manufacturers instructions. RNA concentration and integrity were controlled on a Bioanalyzer system (Agilent) and only RNA samples with RIN (RNA Integrity Number) values > 7 were used for downstream applications. Sequencing libraries were prepared by using the Quantseq 3mRNA-Seq mRNA Library Prep Kit FWD for Illumina (Lexogen) with i7 indexes (Lexogen) according to manufacturers instructions. Libraries were sequenced on an Illumina HiSeq 2500 at single-end.