title: phytotelma metagenome Diospyros kaki 4
design: Extracted DNA was sent for 16S rRNA metagenomics with BioTools Co. Ltd. (Taiwan). Samples had to pass quality control with NanoDrop, Agarose Gel Electrophoresis testing, and Qubit 2.0 DNA concentration quantification. Libraries were constructed on a paired-end Illumina HiSeq 2500 platform to generate 250 bp paired-end raw reads of the V4 region of the 16S rRNA (forward primer 515F: GTGCCAGCMGCCGCGGTAA; reverse primer 806R: GGACTACHVGGGTWTCTAAT).