title: GSM5078735: Developing mouse embryo sections E02E08_S50; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were extracted with a permeabilization buffer, sonicated and fixed. cDNA molecules within nuclei are extracted after tagmentation through the use of denaturants. 3' end capture of polyAdenylated transcripts. In situ reverse transcription with barcoded RT primers followed by second strand synthesis, tagmentation and index PCR gives each nucleus and all the RNA molecules contained within it, a unique combination of barcode sequences. These barcodes are then demultiplexed to create single cells in silico.
attributes.tag: GEO Accession
attributes.value: GSM5078735