title: GSM4646443: A6CRi; Rattus norvegicus; RNA-Seq
library_construction_protocol: Library preparation and RNA sequencing was done by Novogene (Hong Kong). Libraries were made using polyadenylated mRNA isolation, employing NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Three samples had lower RNA amounts and a higher efficiency bead capture was employed for only those, maintaining the same library preparation kit otherwise. Base Quality and Phred score were calculated employing the Illumina CASAVA v1.8 software with more than 92% of all reads for all samples having a Q30. Bowtie v2.2.3 was used to index the reference genome and trimmed paired-end reads were aligned using TopHat v2.0.12.
attributes.tag: GEO Accession
attributes.value: GSM4646443