title: GSM4305244: J_Med_day4; Mus musculus; miRNA-Seq
library_construction_protocol: EVs were isolated from conditioned media using differential ultracentrifugation. Briefly, cellular debris was removed from the media by centrifugation at 200g for 10 min, 2000 g for 10 min, and 10,000 g for 30 min. EVs were pelleted by ultracentrifugation at 100,000 g for 1 hour using the Optima L-90K Ultracentrifuge and SW 32 Ti swinging bucket rotor (Beckman Coulter). The EV pellet was resuspended in TriZol reagent and frozen at -80°C until further analysis. EVs were not pooled for any experiments in these studies. Small RNA libraries were constructed using the NEBNext Small RNA Library Prep Set for Illumina (NEB) according to manufacturer's protocol
attributes.tag: GEO Accession
attributes.value: GSM4305244