title: GSM5343363: smallRNA_Htt KO mESC replicate 1; Mus musculus; ncRNA-Seq
library_construction_protocol: RNA was extracted from cell lines by using TRIzol reagent (Invitrogen) following manufacturer's instructions. The RNA samples were subjected to DNAse I treatment (Ambion) according to manufacturer instructions . RNA library preparation was obtained using the standard Illumina protocols, and sequenced on a Illumina HiSeq 2500 machine using single-end, 50bp reads, obtaining approx 15M reads/sample.
attributes.tag: GEO Accession
attributes.value: GSM5343363