title: RNA-seq of IPSC-dervied human DS astrocytes
design: RNA was extracted from cells using theRNeasy Plus Mini Kit (Qiagen). Sample quality was assessed using a Bioanalyser (Agilent), and sequenced at 12 samples per lane on an Illumina HiSeq4000 for 100 bp paired-end reads at the McGill University and Genome Quebec Innovation Center. The Fast-X toolkit was used to trim the first and last 10 bases of each read. TruSeq specific paired-end adapters and low-quality stretches were removed using Trimmomatic, while poly-A tails were trimmed using PrinSeq. Surviving paired and orphaned reads were separately aligned using STAR to the GRCh37 human genome.