title: GSM5170668: KN99 - gpatch1 day 1, replicate 2; Cryptococcus neoformans; RNA-Seq
library_construction_protocol: Cells were lyophilized and then treated with Trizol and agitated with silica beads in an Omni bead mill homogenizer. RNA was extracted from whole cell extract by phase separation with 1 volume chloroform to produce a phenol:chloroform ratio of 1:1, and then washed with 1 volume chloroform. RNA was precipitated in 50% isopropanol, then recovered by centrifugation for 30 min at 18400xg at 4 C and washed with 70% ethanol. RNA from whole cell extract was polyA selected with Streptavidin MagneSphere® Paramagnetic Particles (Promega) and DNase treated with RNA Clean & Concentrator Kit 5 (Zymo). RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and mixed before being run over multiple lanes
attributes.tag: GEO Accession
attributes.value: GSM5170668