title: GSM2803839: Medip_DP_input; Mus musculus; MeDIP-Seq
library_construction_protocol: E14.5 cortices were dissected and dissociated using the papain-based neural dissociation kit (Milteney Biotec). FAC-sorting was performed at 4ºC in the 4-waypurity mode as previously described (Aprea et al., 2013). DNA was isolated (QIAamp DNA micro kit, Qiagen), sheared (250bp) and ligated to barcoded sequencing adaptors (NEBNext Ultra DNA Library Prep Kit for Illumina, New England Biolabs). 300 ng of DNA from each cell type was pooled and (h)MeDIP performed using the MagMeDIP or hMeDIP kits (Diagnode) according to the manufacturer instructions. The isolated DNA was sheared (250bp) and ligated to barcoded sequencing adaptors (NEBNext Ultra DNA Library Prep Kit for Illumina, New England Biolabs). 300 ng of DNA from each cell type was pooled and (h)MeDIP performed using the MagMeDIP or hMeDIP kits (Diagnode) according to the manufacturer instructions. The immunoprecipitated DNA and nonantibody treated control was quantified, amplified (15 cycles; NEBNext High-Fidelity 2X PCR Master Mix, New England Biolabs) gel size selected (225-325 bp) using the E-Gel EX system (2%) (Invitrogen) and used for 75 bp single read sequencing on a Illumina HiSeq 2000 platform.
attributes.tag: GEO Accession
attributes.value: GSM2803839