title: Effects of paternal circadian disruption on offspring transcription
design: Total RNA was prepared from liver using the TRIzol reagent (Thermo Fisher) according to the manufacturers instructions. RNA concentration and integrity were controlled on a Bioanalyzer system (Agilent) and only RNA samples with RIN (RNA Integrity Number) values > 7 were used for downstream applications. Sequencing libraries were prepared by using either the Nextera Library Prep Kit (Illumina) according to manufacturers instructions. Libraries were sequenced on an Illumina HiSeq 2500 at single-end.