title: Illumina HiSeq 1500 paired end sequencing; Affect of antiviral ribavirin on YFV WT and vaccine strains.
design: Affect of antiviral ribavirin on YFV WT and vaccine strains.
library_construction_protocol: Virus was collected from Ribavirin treated cell supernatant. Supernatant was collected and centrifuged at 1500 rpm for 5 minutes in order to pellet cell debris. The resulting supernatant was aliquoted and frozen at -80 C. Vero cells at 80% confluency were infected at MOI of 0.05 Ribavirin was added at the 2x concentrations stated in sample name in a volume of 750µl. Following incubation at 37°C for one hour, another 750µl MEM supplemented with 2% FBS was added to each well to reach the desired concentration. Virus containing culture media was harvested at 48 hours post-infection. RNA was extracted using the Qiagen QIAamp Viral RNA kit. cDNA libraries were prepared using random hexamer priming (TruSeq RNA Library Prep Kit)
attributes.tag: Experimental Factor: compound
attributes.value: none
attributes.tag: Experimental Factor: phenotype
attributes.value: YFV WT strain