title: RNA-seq of c.elegans: 52hr PTM416
design: worms were synchronized through 3-hour egg laying. Worms were observed every hour after 46 hours until most of them reach L4 stage, this is at timepoint 48 hours. Four hours later, spermatogenesis active worms were collected and kept frozen in -80 oC freezer until RNA extraction. 12 hours later, young adult animals were collected and kept frozen in -80 oC freezer until RNA extraction. Each strain and condition had 3 independent replicates on the same day