title: NextSeq 550 paired end sequencing; RNA-Seq of canine transmissible venereal tumors (CTVT)
design: RNA-Seq of canine transmissible venereal tumors (CTVT)
library_construction_protocol: Biopsies of CTVT-761, 765, 766, 772, 774 and 775 were collected at the Veterinary Hospital ”Dr Halim Atique” - Centro Universitário de Rio Preto (UNIRP), São José do Rio Preto, São Paulo, Brazil. All the procedures carried out in Brazil were approved by the Animal Research Ethics Committee of the São Paulo State University at Jaboticabal, São Paulo, Brazil, under protocol number 24674/2012.Biopsies were collected by surgical excision, after local infiltrations of lidocaine, frozen in liquid N2  CTVT tumour samples collected before therapy (P phase) and after therapy (R) phase. Vincristine sulfate 0.5mg/m2 iv at weekly intervals and biopsies collected at day 0 (before treatment) and at day 28 after the third dose of vincristine. Nucleic acids were extracted from the biopsies using Qiagen RNAeasy Blood & Tissue kit following the manufacturers instructions. 250 ng of total RNA was prepared for sequencing following the Illumina TruSeq mRNA (unstranded) protocol with insert size 150-250
attributes.tag: Experimental Factor: tumor grading
attributes.value: progressive
attributes.tag: Experimental Factor: individual
attributes.value: CTVT-761
attributes.tag: Experimental Factor: compound
attributes.value: none
attributes.tag: Experimental Factor: sampling time point
attributes.value: 0