title: GSM3061556: Th17 + Vehicle 2; Mus musculus; RNA-Seq
library_construction_protocol: CD4+ T cells isolated after treatment and total RNA extracted At least 100ng of DNase-treated total RNA was used to generate polyA-enriched mRNA libraries using NEBNext polyA mRNA sample kits with indexed adaptors (New England Biolabs). Library quality was assessed using the 2100 Bioanalyzer (Agilent) and libraries were quantitated using KAPA Library Quantification Kits (Roche). Pooled libraries were subjected to 75 bp paired-end sequencing according to the manufacturer's protocol (Illumina HiSeq3000). Bcl2fastq2 Conversion Software (Illumina) was used to generate de-multiplexed Fastq files.
attributes.tag: GEO Accession
attributes.value: GSM3061556