title: RNA-Seq of Athelia rolfsii: mycelium
design: For Illumina sequencing of the sRNA, we pooled the RNA from three replicates of the strain. The sRNAs  were purified from a 15%Tris-borate-EDTA (TBE)urea polyacrylamide gel. Next, before sequencing, 5 - and 3 -adaptors were ligated ontoeach sRNA fragment according to the Illumina manufacturers instructions.