title: GSM4304610: PBS_AT2_nonTomato; Mus musculus; RNA-Seq
library_construction_protocol: Established organoids of control or IL-1β-treatment were incubated with dispase (BD Bioscience) for 30-60min. Then, cells were dissociated with TripLE  for 5min, followed by washing with buffer (PBS/0.01% BSA). For SPC or Krt8 lineage-labelled cells, CD45–CD31–EpCAM+Tomato+ cells were sorted at specific time points (at day 14 and day 28 post damage) from PBS or Bleomycin-treated mice (2 mice were pooled for each experiment). For non-lineage-labelled cells isolated from SPCCreERT2;R26RtdTomato mice  in parallel with experiment of SPC lineage-labelled cells, we combined the cells of EpCAM+Tomato– and EpCAM– population with a ratio of 2:1, respectively. The resulting cell suspension (~110,000 cells each) were submitted as separate samples to be barcoded for the droplet-encapsulation single- cell RNA-seq experiments using the Chromium Controller (10X Genomics). Single cell cDNA synthesis, amplification and sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. 10x libraries were multiplexed so that 2 libraries were sequenced per single lane of HiSeq 4000
attributes.tag: GEO Accession
attributes.value: GSM4304610