title: EucalyptustereticornisxEucalyptuscamaldulensis_3
design: GBS Library preparation was performed at Genotypic Technologys Genomics facility at Bangalore. 200 ng of genomic DNA (quantified by Qubit) was subjected to restriction digestion by PstI-HF and MluCI enzymes at 37C for 4 hrs (Refer Figure 1). The restriction site  specific (PstI and MluCI) adaptors were prepared by annealing of the respective single stranded oligos. The resulting restriction digested fragments were directly ligated to a pair of annealed adapters at 16C for 1hr. Adapter ligated fragments were purified by JetSeq magnetic beads (Bio, # 68031). The purified fragments were subjected to PCR amplification (12 cycles) including appropriate dual indexing primers (Nextera XT v2) was performed. The final amplified products were purified using JetSeq magnetic beads (Bio, # 68031).  The Illumina-compatible sequencing libraries were initially quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distribution was analyzed on Agilent TapeStation.