title: GSM4873328: AsPC1; Homo sapiens; RNA-Seq
library_construction_protocol: mRNA was isolated from the cell culture using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. After isolation, the mRNA was treated with Turbo DNAfree Kit (Thermo Fisher Scientific) according to the manufacturer's instructions to decrease the amount of contaminating genomic DNA. The RNA concentration and quality were evaluated by Nanodrop and by using the Agilent2000 Bioanalyzer. RNAseq libraries were prepared using the TruSeq stranded mRNA kit. The RNAseq libraries were sequenced using an Illumina HiSeq 4000 platform, resulting in 150bp single end reads
attributes.tag: GEO Accession
attributes.value: GSM4873328