title: GSM3062929: PTM_M_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq - RNeasy Mini Kit (Qiagen), ChIP-seq - Fresh tissue was homogenized using a hand-held dounce homogenizer, cross-linked in Phosphate buffer saline (PBS) containing 1% formaldehyde for 10 minutes, quenched with 125mM Glycine for 5 minutes and washed three times with PBS. Cross-linked tissue pellet was processed further for chromatin immuoprecipitation using the 'Low cell Chip Kit' (Diagenode; catalog number C01010072) following the manufacturer's protocol. An S. pyogenes Cas9 polyclonal antibody (Diagenode; catalog number C15310258) and an S.aureus Cas9 monoclonal antibody (Diagenode; catalog number C15200230) were used for the pull down. RNA-seq - Nugen Ovations and Nextera XT, ChIP-seq - Rubicon Genomics ThruPLEX DNA-seq kit
attributes.tag: GEO Accession
attributes.value: GSM3062929