title: GSM3897728: RNAseq_TOX_Rep1; Mus musculus; RNA-Seq
library_construction_protocol: In ectopic and enforced expression experiments, in vitro differentiated CD8+ T cells transduced with TOX+GFP or control GFP only (>2 biological replicates each) were sorted on GFP expression 6 days following initial activation to a purity of >98%. To extract RNA, 50,000 cells were resuspended in buffer RLT supplemented with beta-mercaptoethanol and processed with a RNeasy Micro Kit (Qiagen) as per the manufacturer's instructions. Total RNA libraries were prepared using a Pico Input SMARTer Stranded Total RNA-Seq Kit (Takara).