title: GSM4306139: ATACseq.HC1.D22.Rep3; Homo sapiens; ATAC-seq
library_construction_protocol: Differentiated cells were gently pelleted in 1× PBS and resuspended in a 25 μL tagmentation reaction consisting of 1× TD Buffer (10 mM TAPS-NaOH pH 8.1, 5 mM MgCl2, 10% DMF), 2.5 μL Tn5 transposase, 0.1% TWEEN 20, and 0.02% Digitonin, then incubated for 1 hr at 37 °C. Tagmented nuclei were diluted two-fold and incubated for 30 min at 40°C in lysis buffer, comprised of 300 mM NaCl, 100 mM EDTA, 0.6% SDS, 1.6 μg proteinase K. Transposed DNA was isolated by size selection using SPRI-beads and PCR-amplified using 2× KAPA HiFi HotStart ReadyMix (#7958927001, Roche) and Nextera Indexing Primers (#FC-131-2003, Illumina, San Diego, CA, USA). Following PCR amplification, the size-selection step was repeated to enrich for low molecular weight DNA. Sample quality was assessed on a 2100 Bioanalyzer (Agilent Technologies), and libraries were pooled at an equimolar ratio for sequencing on a HiSeq2500 system (Illumina) with 50 bp paired-end chemistry.
attributes.tag: GEO Accession
attributes.value: GSM4306139