title: RNA-seq of Apis mellifera: nurse worker fat body
design: Total RNA was extracted using an RNeasy column (Qiagen). Total RNA integrity was confirmed with an Agilent 2100 Bioanalyzer. RNA-seq libraries were prepared for the six samples using Illumina s TruSeq RNA Sample Preparation Kit as described in Corby-Harris et al. (2014). The resulting fragments of RNA were sequenced using an Illumina HiSeq 2500 and yielded 100 base pair paired-end sequences. The RNA was checked, the libraries were constructed, and the sequencing was performed at the University of Arizona UAGC (http://uagc.arl.arizona.edu/).