title: High throughput amplicon sequenceing of potato associated microbial metagenomes
design: standard Illumina protocoll, two-step pcr, three technical replicates, length selection by gelelectrophoresis if necessary, ,ITS1f-illumina 5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG  CTT GGT CAT TTA GAG GAA GTA A -3 and ITS2-illumina 5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G  GCT GCG TTC TTC ATC GAT