title: GSM4305194: X7953_Stress9; Mus musculus; miRNA-Seq
library_construction_protocol: The caudal epididymis was minced in 1% bovine serum albumin in 3 mL warmed PBS and allowed to sit at room temperature for 30 minutes. The 1% BSA including sperm and epididymal tissue were transferred to a conical tube and incubated in a water bath at 37°C for 30 minutes. The top 2 mL of supernatant was transferred to a new conical tube and incubated at 37°C for 10 minutes. The top 1.5mL of this supernatant was transferred to a new tube and centrifuged for 5 minutes at 4000 rpm at 4°C. The supernatant was removed and the pellet containing mature sperm was flash frozen and stored at -80°C until processing. Sperm samples were not pooled for any experiments in these studies. Small RNA libraries were constructed using the NEBNext Small RNA Library Prep Set for Illumina (NEB) according to manufacturer's protocol
attributes.tag: GEO Accession
attributes.value: GSM4305194