title: GSM4226870: CDX2-CnR-Villus-Rep3; Mus musculus; OTHER
library_construction_protocol: Proximal 1/3rd of small intestine was incubated with 5mM EDTA for 45 minutes to extract villi which were then dissociated to single cells using TrypLE 4x for 30-40 minutes. Single viable cells were isolated using flow sorting. Whole nuclei were immobilized on Concanavalin A beads followed by antibody incubation overnight, pA-Mnase digestion in an ice bath for one hour followed by extraction of DNA using phenol chloroform. Libraries were prepared using NEB Next UltraII and 150bp paired end sequencing was performed on the HiSeq-X (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4226870