title: Frankia diversity in host-plant root nodules is independent of abundance or relative diversity of Frankia in corresponding rhizosphere soils
design: Pooled together into Control (None). This approach was based on 606 bp amplicons generated using primers nifHf1 and nifHr as template in a nested PCR reaction using modified primers nifHf1 and nifH269 to create a 263 bp product. In the nested reaction, primer nifH269 was barcoded, and both primers modified with Illumina adapters, primer pads, and linkers