title: cas9-targeted nanopore sequencing of a native DNA sample
design: 5ug of input DNA was first dephosphorylated. RNP complexes (crRNA:tracrRNA:Cas9) were added to the DNA sample for cleavage. Ten pairs of guide RNA were used to enrich ten human regions.  All DNA ends were dA-tailed, and sequencing adapters were added. The library was cleaned up and finally loaded into the flow cell for sequencing.