title: GSM5342782: NSP1_H1299-2_rnaseq; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-Seq: Total RNA was extracted with QIAzol and ethanol precipitation from 10% of the lysate volume (see above). Sequencing libraries were generated using the SMARTer Stranded RNA-Seq Kit which uses a template switching reverse transcriptase (Takara Bio 634837). Briefly, 100 ng of total RNA were mixed with 1 µL of a 1:10 dilution of ERCC RNA Spike-In Mix controls (Thermo Fisher 4456740). ERCC mix 1 was added to HEK293T and HEK293T-NSP1 and mix 2 was added to HEK293T-NSP2 samples. RNA hydrolysis was done for 4 min and half of the cDNA was amplified for 10 cycles. Samples were sequenced with NovaSeq 6000 S1 PE 100 (Illumina). Sequencing libraries were generated using the SMARTer Stranded RNA-Seq Kit which uses a template switching reverse transcriptase.
attributes.tag: GEO Accession
attributes.value: GSM5342782