title: GSM4933434: GIST peri- tumor tissue from G2 patient; Homo sapiens; RNA-Seq
library_construction_protocol: Tumor tissues, obtained from the laparoscopic mass resection surgery, were immediately sent to undergo the process of single cell suspension preparation. Tissues were cut into small pieces, digested in incomplete RPMI-1640 with endotoxin-free collagenaseⅠ (2 mg/mL, Sigma-Aldrich, St Louis, MO, USA) for 1 hour at 37℃. The digested fragments were filtered through a 100 μm cell nylon mesh (BD Bioscience PharMingen) and washed twice in Hanks' balanced salt solution. Five ml erythrocyte lysis solution was added into the cell suspension for 5 minutes, and then PBS was added to stop and wash twice. 10X Chromium Single Cell 5ʹ Library Construction (CG000086 Rev J)
attributes.tag: GEO Accession
attributes.value: GSM4933434