title: Clean_Euk
design: PCR targeting V1-V3 of 18S rRNA gene (primers Euk1Af-CTGGTTGATCCTGCCAG and Euk516r-ACCAGACTTGCCCTCC). Amplicon was barcoded with 10-bp MID (RL10: ACTACGTCTCT) without discrimination of primer orientation (shotgun chemistry available).