title: Pseudomonas BGI-2 Paired Reads Genome from Batura glacier
design: A pure culture of Pseudomonas sp. BGI-2 was grown in R2A broth at 15C and the genomic DNA was extracted from an overnight culture using UltraClean microbial DNA isolation kit (MO BIO Laboratories). The quality and quantity of the extracted DNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific). The DNA was sequenced by using Illumina MiSeq sequencing. Library was prepared using Nextera XT DNA library prep kit (Illumina Inc., San Diego, CA) according to the manufacturer's protocol and sequencing was performed in a MiSeq 2  250-bp run.