title: 16S rRNA gene sequencing of freshwater metagenome: replicate 1 of reference lake 1 in Baker Lake, NU, Canada (time point 1)
design: Water from the site was filtered through a 0.22 um Sterivex filter. DNA was extracted from filters using the PowerSoil DNA isolation kit. PCR was used to amplify the V4-V5 region of the 16S rRNA gene using the 515F-Y/926R primer set.