title: 454 DNA-Seq of Betula alnoides: young leaves
design: Total genomic DNA  was fragmented into lengths of 3001500 bp by ultrasonication. DNA fragments shorter than 500 bp were removed using an agarose gel DNA purifiationkit. The remaining fragments were then sequenced in a one-sixth run on a Roche 454 GS FLX+ platform. The sequencing library was prepared following Roche 454 standard protocols.