title: gDNA PCR amplicon of mus  musculus: Neuro-2a
design: The on-target and off-target loci  were first amplified by genomic DNA PCR using gene-specific primers with  Illumina adapters. The first PCR products were purified using a commercial  purification kit (Promega, Madison, WI, USA), diluted, pooled, and subjected  to a second round PCR with primers including the index sequences. The final  PCR products were electrophoresed on an agarose gel, showing a single sharp  peak. The purified amplicons were pooled and sent for sequencing using a  MiSeq nano-scale flow cell.