title: bacterial diversity in rainwater - May sample
design: To elucidate the diversity of prokaryotic 16S rRNA gene sequences, bar-coded amplicon sequencing with the GS FLX Titanium was conducted. For amplification of bacterial 16S rRNA gene sequences, the primers 27F and 518R (Akob et al. 2007) were used whose 5' ends were attached to an adapter and sample-specific barcode. These primer sets produced amplicons with length of around 470 to 520 bp. Pyrosequencing of PCR products was performed on a GS-FLX Titanium system (454 Life Sciences, CT) at Macrogen Co. (Seoul, Korea). Samples Barcode sequence April_rainwater ATCAGACACG, April_rainwater (incubated) CTCGCGTGTC, April (filter blank) TCTCTATGCG, May_rainwater AGACGCACTC, May_rainwater (incubated) ATATCGCGAG, May (filter blank) CGTGTCTCTA, July_rainwater AGACGCACTC, July_rainwater (incubated) ATATCGCGAG, July (filter blank) CGTGTCTCTA