title: McMurdo Dry Valley Lakes metagenome; eukaryote
attributes.tag: nucl_acid_amp
attributes.value: DOI:10.1371/journal.pone.0006372
attributes.tag: pcr_primers
attributes.value: 1380FWD:CCCTGCCHTTTGTACACAC; 1389FWD:TTGTACACACCGCCC; 1510REV:CCTTCYGCAGGTTCACCTAC
attributes.tag: target_gene
attributes.value: 18S rRNA
attributes.tag: target_subfragment
attributes.value: V9
attributes.tag: nucl_acid_ext
attributes.value: bead beating alkaline lysis
attributes.tag: pcr_cond
attributes.value: initial denaturation:94degC_4min;denaturation:94degC_45sec;annealing:55degC_45sec;elongation:72degC_1min;final elongation:72degC_10 min;28 cycles