title: GSM4038307: CRFK cells_T02; Felis catus; miRNA-Seq
library_construction_protocol: After 72 h incubation, total RNA of CRFK infected with CPV-2c Laotian stains (CPV) or control was isolated using RNAzol® RT reagent (Sigma-Aldrich, St. Louis, MO, USA) plus Direct-zol™ RNA Miniprep kit (Zymo Research, Irvine, CA, U.S.A.) as manufacture's protocol. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (New England Biolabs, Ipswich, MA, USA) The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumina, San Diego, CA, USA)
attributes.tag: GEO Accession
attributes.value: GSM4038307