title: GSM3061303: C28P3L7-2 N14; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Homogenized plant material of five replicate samples per accession and treatment was pooled to 100 mg separately for the three independent biological experiments which resulted in 36 samples for the analysis. Total RNA was isolated using a TRIzol protocol based on the 'single step' method (Sprenger, Kurowsky, Horn, Erban, Seddig, Rudack, Fischer, Walther, Zuther, Köhl, Hincha & Kopka, 2016). Four μg of each RNA sample was further treated with DNase (RapidOut DNA-removal Kit, Thermo Scientific). Absence of genomic DNA contamination was confirmed by qRT-PCR using intron-specific primers (At5g65080). Finally, RNA quality and integrity were verified with Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Library preperation with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) and library type RNA library including polyA enrichment
attributes.tag: GEO Accession
attributes.value: GSM3061303