title: Raw reads for all samples from the primary screen
design: Sequencing libraries were prepared by nested PCR using the genomic DNA of 293T cells stably infected with ERK1/2 substrate LentiCRISPR library. PCR1 amplifies a ~300 bp region including sgRNA sequence while PCR2 primers contain adapters for illumina sequencing in addition to sequences matching PCR1 product near the sgRNA sequence.