title: Illumina HiSeq 2500 sequencing; Identification and characterization of developmental, tissue, and evolutionary transcription start sites in Bos taurus indicus
design: CAGEseq in bovine Thyroid
library_construction_protocol: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_CAGE-library-preparation_20190903.pdf
attributes.tag: project
attributes.value: FAANG
attributes.tag: assay type
attributes.value: CAGE-seq
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in liquid nitrogen (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 3.0
attributes.tag: extraction protocol
attributes.value: https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2Fcms_055423.pdf&title=bWlyVmFuYSZ0cmFkZTsgbWlSTkEgSXNvbGF0aW9uIEtpdCAoRW5nbGlzaCAp
attributes.tag: library preparation location
attributes.value: Western General Hospital, Edinburgh
attributes.tag: sequencing location
attributes.value: Western General Hospital, Edinburgh
attributes.tag: experiment target
attributes.value: TSS
attributes.tag: cage protocol
attributes.value: ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_CAGE-library-preparation_20190903.pdf
attributes.tag: sequencing primer provider
attributes.value: not provided
attributes.tag: sequencing primer catalog
attributes.value: not provided
attributes.tag: sequencing primer lot
attributes.value: not provided
attributes.tag: restriction enzyme target sequence
attributes.value: CAGCAG
attributes.tag: rna integrity number
attributes.value: 8.2