title: GSM4769390: 033_06_FL_S6; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed and trypsinzed using ReagentPack™ Subculture Reagents (Lonza Bioscience). Single cell suspensions were washed with PBS + 5% FBS, resuspended in 200 µL PBS + 5% FBS, passed through a 70 µm filter, and placed on ice. Cells were counted using trypan blue exclusion on a Luna II (Logos Biosystems). The appropriate numbers of cells to achieve a targeted cell input of 5,000 cells per condition were used to generate the GEMs (Gel Bead-In Emulsion). The Chromium Next GEM Single Cell 3' Gel beads v3.1 kit (10X Genomics, Pleasanton, CA, USA) was used to create GEMs following manufacturer's instruction. All samples and reagents were prepared and loaded into the chip and ran in the Chromium Controller for GEM generation and barcoding. GEMs generated were used for cDNA synthesis and library preparation using the Chromium Single Cell 3' Library Kit v3.1 (10X Genomics) following the manufacturer's instruction.
attributes.tag: GEO Accession
attributes.value: GSM4769390