title: GSM4718869: Tet-TKD T2; Mus musculus; Bisulfite-Seq
library_construction_protocol: GVOs were isolated 48h after PMS injection and injected with either control morpholino or morpholinos targeting Tet1-3. After in vitro maturation and in vitro fertilization, fertilized 2-cell embryos were collected 29hpf and subjected to BS-seq library preparation. For library preparation a Post-Bisulfite Adapter Tagging approach (PBAT) (Lee et. al, PMID: 29224070) was used with few changes. Changes: BD buffer (Qiagen) was used for the desulphonation reaction, biotin-capture was omitted and oligos were changed to Oligo 1: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTNNNNNNNNN and oligo 2: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTNNNNNNNNN, to match the primers from the Nextera XT Index Kit, which were used for the library amplification in the last step using 12 cycles.
attributes.tag: GEO Accession
attributes.value: GSM4718869