title: GSM4718901: IP:H3K9me3 AID-METTL3:-METTL14 dd (+IAA) Rep1; Mus musculus; OTHER
library_construction_protocol: Cells were immobalised to concavalin beads, permeabilised with digitonin and incubated with antibody. After washing, the cells are incubated with A-Mnase protein to digest around the protein-bound DNA. Upon diffussion of DNA-protein complexes out of the cells DNA was collected and size selected with SPRI beads. We used 10ng DNA input for library preparation using Accel-NGS 2S Plus DNA Library Kit (Swift) according to the manufacturer's protocol. Sequencing was performed in 50pb paired-end reads using Novaseq 6000.
attributes.tag: GEO Accession
attributes.value: GSM4718901