title: 16S rRNA gene amplicons of linuron/chlorpropham/chlortoluron enrichment culture initiated with biofilter material
design: To generate 16S rRNA gene amplicons for sequencing, a PCR was performed targeting either the V4 region of the 16S rRNA gene using primers A519F and 802R (Klindworth et al., 2013) or V3-V4 region using primers Bakt_341F and Bakt_805R (Klindworth et al., 2013) amended with overhang adapter sequences. The amplicons were obtained and purified according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol (Illumina, San Diego, CA).