title: RNA seq of PA14_Planktonic_23 deg Biological Replicate 3
design: Planktonic cells were lysed using 700 ul of Qiazol lysis reagent. For disrupting or lysing bacterial cells, the mixture was added onto tubes with beads (Tough Microorganism Lysing Mix (RNase+DNase free, size- 2mL X 0.5mm Verre)) and lysed in a bead mill. RNA was then extracted using the RNeasy minikit (Qiagen) according to the manufacturers recommendations, and the RNA solution was digested with the RNase-free DNase set (Qiagen), followed by on-column DNase digestion to eliminate any remaining traces of genomic DNA. The purified RNA was quantified using a Take3 plate reader (Synergy H1 microplate reader, Biotek). Samples were then sent to Genewiz for library prep and Illumina HiSeq