title: GSM4038621: 443CD4D0; Homo sapiens; RNA-Seq
library_construction_protocol: MACS-purified CD4+ T cells were homogenised in QIAshredder (QIAGEN) columns, followed by RNA extraction using the RNeasy Mini Kit (QIAGEN) according to manufacturer's instructions. Total RNA was treated with the RNase-free DNase Set (QIAGEN). mRNA was isolated using the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs). Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).
attributes.tag: GEO Accession
attributes.value: GSM4038621