title: scRNA-seq of mu: CD45+ immune cell
design: Panc02 tumor cells were injected subcutaneously into male C57BL/6 mice at 1 x 106 cells per 200ul on bilateral flanks, respectively. Tumor on the left flank receives RFA treatment, while the tumor on the right side did not receive any intervention. On day 3 after RFA treatment, tumor on the right side (non-RFA side) were isolated, digested and isolated CD 45+ cells. Libraries were constructed using Single Cell 5 Library Kit.