title: DNA-Seq of  Deinococcus radiodurans R1 erythromycin resistant mutant
design: INCPM DNA-Seq was performed as previously described (Blecher-Gonen et al., 2013; https://www.ncbi.nlm.nih.gov/pubmed/23429716) with the following modifications: 8 cycles of PCR were performed using 2X KAPA HiFi  ready mix (Kappa Biosystems, Inc. Wilmington, MA, USA) with the following program: 2min at 98C, 8 cycles of 20sec at 98C, 30sec at 55C, 60sec at 72C following by 72C at 10min.