title: 16S rRNA gene sequencing of heavy and light fractions of 13CO2 incubated pea plants
design: Sequences were depleted of barcodes and primers then short sequences < 200bp were removed, together with sequences with ambiguous base calls, and sequences with homopolymer runs exceeding 6bp. Sequences were then denoised and operational taxonomic units (OTUs) were defined as clustering at 97% similarity, following removal of singleton sequences and chimeras [3843]. Final OTUs were taxonomically classified using BLASTn against a curated database derived from GreenGenes, RDPII and NCBI