title: Drosophila melanogaster Florida 1997
design: 60 pooled males of 5.2 ug of DNA was prepared and submitted to the New York Genome Center (NYGC) for library preparation and sequencing. The lines showed some degradation with a bimodal size distribution with peaks at ~300bp and ~2,500 bp. The NYGC used a PreCR Repair Mix to perform fragment repair as a precautionary measure. Size selection was then performed to separate the two major peaks, and the 2,500 bp fragments only were sheared. Both the unsheared and sheared fragments were then used for library preparation before sequencing on a single lane of an Illumina HiSeq 2500 1TB upgrade to generate paired-end 2 x 125bp reads.