title: Whole genome sequencing of Shiga toxin-producing Escherichia coli O157:H7 from pigs, cattle and pork production environment
design: The isolate was grown in half-strength Brain Heart Infusion Broth (Oxoid, Mississauga, ON, Canada) at 35oC for 18  2h. The DNA of each culture was extracted using MasterPure Complete DNA and RNA purification kit (Lucigen, Middleton, WI, US) following the manufactures instruction. The DNA samples were sent to Genome Quebec Innovation Centre, Montreal, QC, Canada for sequencing. Sequencing library was constructed using NEBNext Ultra II DNA Library Prep Kit and sequenced for 1502 cycles using Illumina HiSeq4000 with read depth > 300.