title: bacteria_16S_rRNA_sequences_Hex5
design: All samples were pooled together with equal molar amount from each sample. The sequencing samples were prepared using TruSeq DNA kit according to manufactureĄŻs instruction. The purified library was diluted, denatured, re-diluted, mixed with PhiX (equal to 30% of final DNA amount) as described in the Illumina library preparation protocols, and then applied to an Illumina Miseq system for sequencing with the Reagent Kit v2 2ĄÁ250 bp as described in the manufacture manual.