title: GSM5169156: Sample8_DP_2; Mus musculus; RNA-Seq
library_construction_protocol: Naïve T cells were sorted directly in Trizol LS The Takara pico RNA protocol was used.  In short, 10ng total RNA was used as input. After fragmentation at 94°C for 4min, SMART technology was used to make the directional cDNA library. The ribosomal cDNA was then depleted and P5, P7 Illumina adptors were added to the library via PCR. ribosomal cDNA depletion, directional cDNA library.
attributes.tag: GEO Accession
attributes.value: GSM5169156