title: GSM3667084: B16_3; Mus musculus; RNA-Seq
library_construction_protocol: Smart-seq2 protocol was applied. After 10 lymphatic endothelial cells were placed into the lysis buffer by mouth pipette, in reverse transcription reaction, a poly(T) tail was added to the 5' end of the first-strand cDNAs. After the first-strand reaction, the cDNAs were amplified by 18 cycles for the library construction. The amplified cDNA was fragmented to approximately 300bp by Covaris S2, and KAPA Hyper Prep Kit was used to generate sequence libraries. Paired-end 150 bp sequencing was further performed on an Illumina HiSeq 4000 platform (sequenced by Novogene).
attributes.tag: GEO Accession
attributes.value: GSM3667084