title: Illumina 16S amplicon sequence of bacteria in maple seedling roots
design: V5-V6 region of the bacterial 16S ribosomal RNA gene using the chloroplast-excluding primers 799F-1115R.  All amplified PCR samples were cleaned and normalized using the Invitrogen Sequalprep PCR Cleanup and Normalization Kit. DNA library was sequenced using an Illumina MiSeq Sequencer with the 2 300 bp paired end platform using the Illunima MiSeq Reagent Kit v3.