title: amplicon-seq of bacterial 16S: sediment
design: The hypervariable region V3-V5 of the bacterial 16S rRNA gene was amplified by PCR using the barcoded universal primers 338F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) .The purified PCR product was first quantified,  and the pair-end libraries were constructed using the TruSeq Nano DNA Library Prep Kit.