title: GSM1847273: E15.5 preSertoli cells_RNA-seq 1; Mus musculus; RNA-Seq
library_construction_protocol: FACS purified E15.5 preSertoli cells were collected from ~165 embryos and pooled into 3 independent biological replicates (DM1, DM2, DM3), each containing ~1 million cells. RNA isolation was performed using the RNeasy Micro Kit according to the manufacturer's protocol (Qiagen). The RNA was DNaseI digested. QC and quantitation was performed on the Thermoscientific NanoDrop 2000 and the Agilent Bioanalyzer. Poly-A enriched mRNA libraries were generated from 0.8-1 µg of RNA using the standard Illumina Tru-Seq V2 protocol, then quantitated on the Agilent Bioanalyzer and adapted with index 23, 25 or 27. 7pM of the resulting library pool was run in a single Illumina HiSeq2000 lane to generate 50 bp single-end reads.
attributes.tag: GEO Accession
attributes.value: GSM1847273