title: tunable Genotype by Sequencing (tGBS) library of Cucurbita argyrosperma subsp. argyrosperma from Ek Balam, Yucatan (A_EK6)
design: The seed was germinated in a greenhouse and total DNA was extracted from fresh leaves using a DNeasy Plant MiniKit (Qiagen). The extracted DNA was sequenced by Data2Bio LLC using the tunable Genotyping by Sequencing (tGBS) method with an Ion Proton instrument and two restriction enzymes (Sau3AI/BfuCI and NspI).