title: Illumina HiSeq 2000 paired end sequencing; IMPACT OF INTERLEUKIN 10 DEFICIENCY ON INTESTINAL EPITHELIUM RESPONSES TO INFLAMMATORY SIGNALS
design: IMPACT OF INTERLEUKIN 10 DEFICIENCY ON INTESTINAL EPITHELIUM RESPONSES TO INFLAMMATORY SIGNALS
library_construction_protocol: Small intestinal organoid cultures were growing in 24-well plates SI organoids were growing in matrigel with Intesticult medium and they were split at least once before the experiment addition of 40ng/ml of recombinant mTNF in the culture medium Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina) from 1 Âµg total RNA of each sample and sequenced on an Illumina HiSeq2000 (100-nucleotide paired-end reads).  RNA extraction and purification from enteroids was performed using the RNeasy mini-kit (Qiagen), as per manufacturer instructions.  Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina)
attributes.tag: Experimental Factor: time
attributes.value: 0.5
attributes.tag: Experimental Factor: stimulus
attributes.value: TNF, 40 nanogram per milliliter