title: GSM4038330: Rv1720c.mutant_Glycerol_1; Mycobacterium tuberculosis; RNA-Seq
library_construction_protocol: cDNA libraries were prepared from the resultant rRNA depleted RNA and 1 ul of a 1:500 dilution of ERCC RNA Spike in Controls (Ambion® Thermo Fisher Scientific, Waltham, MA, USA) using Lexogen SENSE Total RNA-Seq Library Prep Kit (Lexogen GmnH, Vienna, Austria) according to manufacturer's protocol except with 21 PCR cycles. The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2x51 cycles on an Illumina HiSeq 2000 system (Illumina, San Diego, CA, USA) (16 samples/lane).
attributes.tag: GEO Accession
attributes.value: GSM4038330