title: GSM5176583: NICD_TCFKO_1 [RNIC-Tg-1_S7]; Mus musculus; RNA-Seq
library_construction_protocol: Cells were isolated from sorted bone marrow LSK and RNA was harvested using PicoPure™ RNA Isolation Kit (Thermo Fisher Scientific, KIT0204) according to manufacturer's instructions We performed cDNA library with the "Illumina TruSeq RNA Library Prep kit" We then performed a Nextera XT library prep according to Illumina protocol 15031942 Rev. E. We quantified the libraries by qubit DNA HS and performed profile analysis by TapeStation 4200. We performed a single end 75nt sequencings on NextSeq 500.
attributes.tag: GEO Accession
attributes.value: GSM5176583