title: RNAseq of Vibrio fischeri ES114 hfq mutant: 12 h LSMMG
design: Total RNA was extracted in triplicate and treated with Dnase to remove contaminating DNA. rRNA was removed and the sample was processed using a clean and concentrate kit. The remaining mRNA was pooled for each sample. High quality mRNA was converted to cDNA. Quality and Quantity of cDNA was checked before submission for sequencing.