title: 4A2S24
design: We assessed the performance of five common approaches used for bacterial DNA extraction from breast milk samples. These included four based on commercial kits (QIAgen PowerLyzer PowerSoil DNA Isolation, Sigma Aldrich GenElute Bacterial Genomic DNA Kit, QIAamp DNA Stool Mini Kit, and QIAamp DNA Stool Mini Kit with bead beating) as well as a phenol-chloroform based extraction method.