title: RAD-seq of Cyprinus carpio
design: Restriction by PstI and 96-plex multiplexing according to the GBS protocol by elshire et al. For each 96 plex, 91 individuals from one family, the four parents of both families, and one blank control sample were used. Each 96-plex was sequenced on a single lane of Illumina HiSeq2500 flow cell to obtain 100 bp single end reads.