title: GSM1215359: MZ_rep2; Zea mays; RNA-Seq
library_construction_protocol: RNA-seq libraries were prepared as follows. Approximately four micrograms of total RNA was used as input. Each sequencing library was constructed using the TruSeq RNA Sample Prep Kit V2 (Illumina, San Diego, CA) according to manufacturer’s instructions with the following modifications to confer strand-specificity. The polyA-selected RNA was used for first strand synthesis and the resulting cDNA:RNA hybrid was purified using RNAClean XP beads (Beckman, Brea, CA). The second strand synthesis was performed using a dNTP mix containing dUTPs (10mM dATPs, 10mM dGTPs, 10mM dCTPs, and 20mM dUTPs) and DNA Polymerase I (New England Biolabs, Ipswich, MA). The purified ligation products were incubated with Uracil DNA Glycosylase (Fermentas) before PCR amplification.
attributes.tag: GEO Accession
attributes.value: GSM1215359