title: 16S amplicon of Thalassia hemprichii of inside leaf at medium stress treatment biological replicate 1
design: Bacterial genomic DNA samples were extracted from the insideleaf. The V3-V4 hypervariable regions of the bacterial 16S rRNA genes were amplified from genomic DNA of each sample by Polymerase chain reaction (PCR) with bacterial primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). The PCR products were extracted from 2% agarose gel, further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), and quantified using QuantiFluor-ST (Promega, USA) following the manufacturers guidelines.