title: GSM4989036: Human iPSC FXS2_1; Homo sapiens; RNA-Seq
library_construction_protocol: Samples for RNA-seq were extracted with Trizol and single cells for single cell RNA-seq were obtained with Accutase from organoids. The UV crosslinked samples fro eCLIP were lysed in lysis buffer (50 mM Tris-HCl pH7.5, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate and protease inhibitor) on ice for 10-30 min followed by digestion with RNase I and DNase (Invitrogen) at 37C for 5 min. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: GEO Accession
attributes.value: GSM4989036