title: GSM4403371: HighRunner_line3_GCCAAT [Runner1]; Mus musculus; RNA-Seq
library_construction_protocol: The VNO tissues were harvested from 3 male mice from each of the 4 HR and 4 control lines, immediately transferred to RNA later (Sigma-Aldrich), then stored at −80˚C until use for RNA-seq. VNO tissues from the same line of mice were pooled and homogenized in Trizol Reagent (Life Technologies, Carlsbad, CA) and processed according to the manufacturer's protocol. Trizol-purified RNA samples were quantified using Qubit1 2.0 (Life Technologies). The integrity of isolated RNA was measured by the 28S/18S rRNA analysis using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara CA) with RNA Nano chip (Agilent Technologies, Palo Alto, CA). Samples had RNA integrity number values of at least 8.30. RNA libraries were prepared for sequencing using the Ultra II Directional RNA Library Prep kit (NEB).
attributes.tag: GEO Accession
attributes.value: GSM4403371