title: GSM4874349: Non-Tg [SAM24359109]; Mus musculus; RNA-Seq
library_construction_protocol: Mice were perfused with PBS. Hippocampi and fibria were dissected out and dissociated with papain-containing Miltenyi kit (130-092-628). Live single cells were enriched by FACS, and single cell suspensions were subjected to 10X Genomics procedure (Chromium™Single Cell 3' Reagent Kit v2 (10X Genomics PN-120237) for single cell RNA extraction and RT. Library was contructed with Chromium™Single Cell 3' Reagent Kit v2 (10X Genomics PN-120237) following manufacturer's user guide. cDNA amplification was done with 10 cycle PCR, and sample index PCR was done with 12 cycles. Libraries were sequenced by HiSeq4000 (Illumina) following 10X Genomics sequencing specification.
attributes.tag: GEO Accession
attributes.value: GSM4874349