title: GSM4038313: Mutant UV10046 pH 5.5; Aspergillus carbonarius; RNA-Seq
library_construction_protocol: Total RNA was isolated from fungal samples as per the manufacturer protocol of Qiagen Rneasy Plant Mini Kit (#74903) paired-end sequencing library preparation using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770S) and NEBNext Singleplex (NEB #E7350) Oligos for Illumina as per the protocol described by the manufacturer The library preparation was initiated with the capture of mRNA from Total RNA using Oligo dT beads, fragmentation, first Strand cDNA synthesis, second strand synthesis, end-repairof the double-stranded cDNA and Adaptor ligation, indexing and final library  amplification. The libraries were purified using SPRISelect beads. The amplified libraries were analyzed in 4200 TapeStation (Agilent Technologies) using kit as per manufacturer's instructions