title: Ion Torrent Proton sequencing; RNA-seq of the zebrafish (danio rerio) brain after developmental exposure to 17α-ethinylestradiol
design: RNA-seq of the zebrafish (danio rerio) brain after developmental exposure to 17α-ethinylestradiol
library_construction_protocol: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer (0.8 ml/sample) (Sigma-Aldrich, Germany) EE2 exposure. Fish exposed to 0, 2.14 or 7.34 ng/L EE2 for 80 days starting 1 day after fertilization Ion Whole transcriptome analysis kit on RiboZero (eukaryote) depleted total RNA
attributes.tag: Experimental Factor: male
attributes.value: sex
attributes.tag: Experimental Factor: 17-ethinylestradiol
attributes.value: compound
attributes.tag: Experimental Factor: 2.14
attributes.value: dose