title: GSM4873109: 3 prime HS1 deleted HUDEP2 clone B6 ATAC-seq; Homo sapiens; ATAC-seq
library_construction_protocol: Upon 5 days of HUDEP-2 differentiation, 50,000 cells were harvested and permeabilized in 50 uL of cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). The transposition reaction was carried out at 37oC for 30 minutes with agitation in 50uL volume containing 25uL of 2X TD buffer and 2.5uL of Nextera Tn5 transposase. DNA was purified with Qiagen MinElute PCR purification kit. Library amplification was done with KAPA HiFi HotStart ReadyMix PCR kit, and the resulting libraries were purified with Qiagen MinElute PCR Purification Kit.
attributes.tag: GEO Accession
attributes.value: GSM4873109