title: iCLIP of drosophila Mi-2 without barcode
design: Individual nucleotide-resolution UV-crosslinking and immunoprecipitation 2 was performed as described in (Buchbender et al., 2020). Briefly, S2 cells expressing GFP-tagged dMi-2 (Lenz et al., 2021) and untagged S2 cells were washed with cold 1X PBS. Protein-RNA cross-linking was performed at 254 nm UV light (300 mJ/cm2) using a Stratagene Stratalinker. These read did not match one of the used multiplexing barcodes.