title: Gluten-free offspring
design: GF_offspring_01 .=> AGTACGCTAT GF_offspring_02 .=> ATAGAGTACT GF_offspring_03 .=> CACGCTACGT GF_offspring_04 .=> CAGTAGACGT GF_offspring_05 .=> CGACGTGACT GF_offspring_06 .=> TACACACACT GF_offspring_07 .=> TACACGTGAT GF_offspring_08 .=> TACAGATCGT GF_offspring_09 .=> TACGCTGTCT GF_offspring_10 .=> TAGTGTAGAT GF_offspring_11 .=> TCGATCACGT GF_offspring_12 .=> TCGCACTAGT GF_offspring_13 .=> TCTAGCGACT GF_offspring_14 .=> TCTATACTAT GF_offspring_15 .=> TGACGTATGT GF_offspring_16 .=> TGTGAGTAGT  The amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche).