title: GSM4306732: XX1 neurons; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from hiPSCs and NPCs using the miRNeasy Micro Kit (QIAGEN, Hilden, Germany). For quality control of RNA or DNA yield and library synthesis products RNA ScreenTape kit, D1000 ScreenTape kit (both from AGILENT TECHNOLOGIES), Qubit® RNA HS Assay kit, Qubit® DNA HS Assay kit (Invitrogen) were used for each specific step. mRNA libraries were prepared from 1 mg RNA using KAPA Stranded mRNA-Seq Kit, with mRNA Capture Beads (kapabiosystems, KK8421). mRNA libraries were prepared from 1 mg RNA using KAPA Stranded mRNA-Seq Kit, with mRNA Capture Beads (kapabiosystems, KK8421). Each library was eluted in 20µl elution buffer, adjusted to 10 mM, then 10µl (50%) from each sample were collected and pooled into one tube. The multiplex samples pool (1.5 pM including PhiX 1%) was loaded on NextSeq 500/550 High Output v2 kit (75 cycles) cartridge, and loaded on NextSeq 500 System (Illumina)
attributes.tag: GEO Accession
attributes.value: GSM4306732