title: GSM4873511: org_adult_rep1_atac; Mus musculus; ATAC-seq
library_construction_protocol: Fetal spheroids and adult organoids were released from Matrigel and resuspended in TrypLE Express Enzyme (GIBCO) and incubated at 37°C until a single cell suspension was obtained. After centrifugation, cells were resuspended in PBS with 1% BSA and incubated with fluorescent-conjugated primary antibody EpCAM-APC (Supplementary table 1), for 30 min on ice. After washing, DAPI (Sigma; 1 µM) was added and 50 000 live (EpCAM+ DAPI-) cells were subsequently sorted on a FACS Aria I (BD Bioscience) cell sorter. The cells were lysed and after centrifugation resuspended in transposase reaction mix and incubated for 30 min at 37°C. After purification, the eluted DNA was PCR amplified with unique barcode (TAAGGCGA, CGTACTAG, TCCTGAGC and TAGGCATG) containing PCR primers for multiplexing of samples: and purified. The samples were paired-end sequenced with Illumina NextSeq.
attributes.tag: GEO Accession
attributes.value: GSM4873511