title: small RNA-Seq of acyrthosiphon pisum: mix-stage male wholebody
design: We collected samples from three developmental stages: embryos, first instar nymphs, second instar nymphs. Each sample was a pool of 15-20 individuals. We extracted total RNA separately from each pool using a standard Trizol protocol plus DNase1 treatment and then pooled them in equal proportions in order to sequence a single sample. Small RNAs were size selected, processed into an Illumina TruSeq Small RNA library