title: amplicon-seq of archaeal 16S: sediment
design: The hypervariable region of the archaeal 16S rRNA gene were amplified using PCR with the barcoded universal primers Arch344F (5  - ACGGGGYGCAGCAGGCGCGA-3  ) and Arch915R (5  -GTGCTCCCCCGCCAATTCCT-3  ) .The purified PCR product was first quantified,  then the pair-end libraries were constructed using the TruSeq Nano DNA Library Prep Kit.