title: Mangifera indica 'TOMMY ATKINS' transcript reads from seed coat
design: Seven strand-specific RNA libraries were prepared for indexed Illumina sequencing.  These represented six different organs of the Tommy Atkins cultivar individual N2-1-04-07 collected at the USDA ARS Subtropical Horticulture Research Station in Miami, FL.  Libraries prepared at Indiana University used Illumina TruSeq Stranded v2 kit reagents according to the manufacturer, with input of 4 micrograms total RNA per library. Libraries were sent to the University of Illinois Roy J. Carver Biotechnology Center for pooling and sequencing on a HiSeq2000 instrument for 2 x 101 nt paired reads, using TruSeq SBS sequencing chemistry version 3. Data were processed and fastq files generated using CASAVA 1.8.2.