title: Amplicon seq, adenine base editing, SGCA c.157G>A mutation, off-target 4, human muscle stem cells
design: PCR amplicon off-target site 4, AJAP1 locus. Primers: fwd(5>3) CCCCGGTTGGTTTGAAAACG / rev(5>3) TCAGGTGAGGCTTGTGAGG. Q5 DNA Polymerase. Amplicon checked with 2100 Bioanalyzer using DNA 1000 Kit (Agilent).