title: Full genome sequencing of TDA exposed Vibrio anguillarum
design: DNA was fragmented by Covaris, which was then combined with End Repair Mix, incubate 30 min at 20°C. End-repaired DNA was purified with QIAquickPCR Purification Kit (Qiagen), A-Tailing Mix was added, incubate 30 min at 37°C. Purified Adenylate 3'Ends DNA and Adapterand Ligation Mix was combine, incubate 15 min at 20°C. Adapter-ligated DNA was selected by running a 2% agarose gel, purified with QIAquick Gel Extraction kit (QIAGEN). PCR amplification was performed several rounds with PCR Primer Cocktail and PCR Master Mix. The PCR product was the se selected by running another 2% agarose gel to recover the target fragments. Purified with QIAquick Gel Extraction kit (QIAGEN).