title: Soil metagenome of background
design: The sequencing library is prepared by purified 16S rRNA gene amplicon, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified. The library construction and sequencing was conducted by Macrogen (Seoul, South Korea) following the manufacturer's protocol of illumina.