title: RNA-Seq of mus musculus: Neuro-2a cells
design: RNA library preparation was performed using NEBNext Ultra II Directional (stranded) RNA Kit for Illumina (NEB #E7760L New England Biolabs) with an initial input of 100ng ng extracted RNA per sample, measured using Qubit RNA HS reagents (#Q32852 Invitrogen) for fragmentation, cDNA synthesis and amplification. Depletion of ribosomal RNA (rRNA) was carried out with NEBNext rRNA Depletion Kit (human, mouse, rat) from New England Biolabs (#E6310X). NEBNext Multiplex oligos indexes kits (E7335L, E7500L and E7710L) from New England Biolabs were used to barcode each library following the manufacturer protocol.