title: GSM2102844: JF-7; Mus musculus; RNA-Seq
library_construction_protocol: First mandibular molars at PN7.5 from Gli1-CreER;Bmpr1afl/fl and littermate Bmpr1afl/fl control mice were dissected out, and the apical half dental mesenchymel was cut with a fine blade and transferred to RNA stabilization reagent (Qiagen, 76104). Total RNA was extracted with an RNeasy Mini Kit (Qiagen, 74134). RNA extracted from cells was polyA+ labelled and used to generate libraries  following the manufacturer’s protocols (Illumina).  The modified cDNA molecules were subjected to sequencing by Illumina Next-Seq 500 system
attributes.tag: GEO Accession
attributes.value: GSM2102844