title: GSM1849277: H3K4me3 chIP-seq in WT yeast, rep #1; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Sporulating cell samples at different time point were cross-linked with 1% formaldehyde at room temperature for 15 minutes immediately after harvesting. Chromatin immunoprecipitation (ChIP) assays were performed as described in (Govin, J., Schug, J., Krishnamoorthy, T., Dorsey, J., Khochbin, S., and Berger, S.L. (2010b). Genome-wide mapping of histone H4 serine-1 phosphorylation during sporulation in Saccharomyces cerevisiae. Nucleic acids research 38, 4599-4606.) and followed by library preparation. ChIP-seq libraries were prepared using NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® in accordance with the manufacturer’s instructions and subjected to single-end sequencing using an Illumina® NextSeq instrument.
attributes.tag: GEO Accession
attributes.value: GSM1849277