title: RNA-Seq of Homo sapiens: 13-yr old male fibromatic gingiva
design: mRNA sequencing libraries were prepared according to the manufacturers instructions (Illumina Truseq RNA Prep Kit v2). mRNA was purified and fragmented from total RNA (1 g) using poly-T oligo-attached magnetic beads with two rounds of purification. RNA fragments primed with random hexamers were reverse transcribed into first strand cDNA using reverse transcriptase and random primers.