title: GSM4873714: SmallRNA_Tv01; Trichomonas vaginalis; ncRNA-Seq
library_construction_protocol: Total RNA of each isolate was extracted employing TRIzol Reagent (Invitrogen, Shanghai, China) and treated with DNase I to remove any genomic DNA contamination. Small RNA molecules were ligated to 5' and 3' adaptors consecutively and then converted to cDNA by reverse transcription followed by polymerase chain reaction (PCR) amplifications. Approximately 2.34 femtomole of reverse transcription - PCR (RT-PCR) products per sample were sequenced directly by Illumina NextSeq 500 system (Illumina, USA) at 50 base pairs (bp) single-read by Aksomics Inc (Shanghai, China)
attributes.tag: GEO Accession
attributes.value: GSM4873714