title: GSM4873126: 3 prime HS1 inverted HUDEP2 clone A2 CTCF Cut&Run; Homo sapiens; OTHER
library_construction_protocol: CTCF Cut&Run was performed according to published protocol 5. Briefly, 500,000 of HUDEP2 cells upon 5 days of differentiation were immobilized with BioMag Plus Concanavalin A (BangsLabs, Inc). Cells were permeabilized and incubated with CTCF antibody (ab70303, Abcam) for overnight. pA-MNase (a gift from Dr. Steven Henikoff) was then added to the cells and incubated for 30 min in metal block on ice. MNase reaction was stopped, chromatin was released, and DNA was extracted. Accel-NGS 2S Plus DNA library kit (21096, Swift Bioscience) was used to construct the library followed by 2S combinatorial dual indexing kit (28096, Swift Bioscience). Cut&Run
attributes.tag: GEO Accession
attributes.value: GSM4873126