title: GSM3667073: 188CRS7_Week2_seedling_ELF3_OX_ZT20_20C; Brachypodium distachyon; ChIP-Seq
library_construction_protocol: 3g seedlings for each set were fixed under vacuum for 20 min in 1xPBS  (10 mM PO43−, 137 mM NaCl, and 2.7 mM KCl) containing 1% Formaldehyde (F8775 SIGMA). The reaction was quenched by adding glycine to a final concentration of 62 mM. Chromatin immunoprecipitation (ChIP) was performed as described (12), with the exception that 100 µl FLAG M2 agarose affinity gel antibody was used (A2220 SIGMA-Aldrich) per sample. Sequencing libraries were prepared using TruSeq ChIP Sample Preparation Kit (Illumina  IP-202-1024) and samples sequenced on NextSeq500 machine from Illumina using NextSeq® 500/550 High Output Kit v2 (75 cycles) TG-160-2005. Sequence reads were analysed using in-house pipelines. The efficiency of ChIP materials was assessed by qPCR prior to in-house library preparation. Sequencing libraries were prepared using TruSeq ChIP Sample Preparation Kit (Illumina  IP-202-1024) and samples sequenced on NextSeq500 machine from Illumina using NextSeq® 500/550 High Output Kit v2 (75 cycles) TG-160-2005. Sequence reads were analysed using in-house pipelines. The libraries were sequenced at the Sainsbury Laboratory Cambridge University Services sequencing facility.
attributes.tag: GEO Accession
attributes.value: GSM3667073