title: First transcriptome analysis of an egg parasitoid Trichogramma chilonis
design: Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” ( Part # 15026495 Rev. F ). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 3 minutes at elevated temperature (94 o C) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (MAGBIO, Cat# AC-60050). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Qubit and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).