title: GSM5176547: AKT Round 1 Tumor 3 gDNA barcodes; Homo sapiens; OTHER
library_construction_protocol: Teratomas and tumors were dissociated using the standard GentleMACS human tumor dissociation protocol. For scRNA-seq, dissociated cells were resuspended in PBS + 0.04% BSA and processed on the 10X Genomics Chromium system using standard protocols. For bulk RNA extraction, dissociated cells were pelleted, RNA extracted using a Qiagen RNeasy kit and RNA-seq libraries prepared. For barcode amplification from genomic DNA, dissociated cells were pelleted, genomic DNA extracted using the Qiagen DNeasy kit and barcodes were PCR amplified and prepared for sequencing. scRNA-seq Libraries were constructed using the standard Chromium Single Cell 3ʹ Reagent Kits v3 workflow (10x Genomics). Bulk RNA-seq libraries were constructed using NEBNext Ultra RNA Library Prep Kit.
attributes.tag: GEO Accession
attributes.value: GSM5176547