title: GSM2101716: vivo_2nd_fol._oocyte_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Directional RNA-seq library was constructed, as described previously (Hamazaki et al. 2015). Briefly, poly(A)+ RNA were purified from 100-15,000 cells or oocytes (ESCs; 10,000-13,200, PGCLCs day 6; 10,000, PGCLCs re-agg day 3; 5,300-15,000, secondary follicle oocyte; 337-812, MII oocyte; 99-134), using the Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Purified RNAs were subjected to library construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). cDNA were enriched by 12-cycles PCR. Sequencing of the libraries was performed with HiSeq2000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM2101716