title: RNA-seq of homo sapiens: HPV transfected JAR cells (placenta)
design: Total RNA extract is treated with Dnase I and  Oligo(dT) are used to isolate mRNA. mRNA is fragmented and cDNA synthesized. Short fragments are purified and resolved in EB buffer for end reparation and single nucleotide A addition, followed by adapter connection. Suitable fragments are selected for PCR.