title: BARBEKO library Gene fitness screening  in RPE1-WT by CBE at MOI3 Day 15
design: BBK, RPE1-WT, fitness, MOI3, CBE, Day 15; The genomic DNA of each replicate was extracted from cells using the DNeasy Blood and Tissue kit (Qiagen) and the sgRNA regions were PCR amplified by KAPA Hot Start High-Fidelity DNA Polymerase with 26 cycles of reaction using several pairs of primers. The PCR products were pooled together and purified with DNA Clean & Concentranter-5 (Zymo Research Corporation), followed by NGS analysis