title: RNA-Seq of Hyposoter didymator ovaries from pupae stage 1
design: Total RNAs were extracted from pools of 40 to 50 ovaries dissected from females at pupal stage 1 (~3 day-old pupae) using the Qiagen RNeasy extraction kit according to manufacturers protocol. Following extraction, total RNAs were systematically submitted to DNase treatment with the TURBO DNA-free Kit (Life Technologies).