title: GSM4874417: Healthy gender-matched control for DBF4; Homo sapiens; RNA-Seq
library_construction_protocol: RBC depletion using EasySep™ RBC Depletion Reagent (StemCell Technologies) Single cell RNAseq libraries were generated using a commercial droplet platform (Chromium system; 10X Genomics). Briefly, the EasySep™ RBC Depletion Reagent (StemCell Technologies) was used to deplete erythrocytes from whole bone marrow samples collected in heparinised tubes according to manufacturer's instructions. The cell count and the viability of the bone marrow cells were assessed using LUNA dual florescence cell counter (Logos Biosystems) and as per the 10X recommendations (Single cell 3' reagent kits v2 user guide; CG00052 Rev B), we aimed at a target recovery of 6000 cells for all the samples. Post cell count and QC, the samples were immediately loaded individually onto the Chromium Controller. Single cell RNAseq libraries were prepared using manufacturers recommendations, and at the different check points the library quality was assessed using Qubit (ThermoFisher) and Bioanalyzer (Agilent). With a sequencing coverage target of 50,000 reads per cell, single cell libraries were sequenced on Illumina HiSeq4000 platform using paired-end sequencing workflow and with recommended 10X; v3 read parameters (28-8-0-91 cycles).
attributes.tag: GEO Accession
attributes.value: GSM4874417