title: GSM4305750: THP-1 cells, 12hr INCB059872 B; Homo sapiens; OTHER
library_construction_protocol: Nuclear run-on assays were performed with ATP, UTP, GTP, and biotin-11-CTP. Nuclei were lyzed with TRIzol, nuclear RNA was extracted and fragmented, and biotinylated RNA was purified with strepavidin magnetic beads. Library construction included 3' adaptor ligation, 5' repair, 5' adaptor ligation, reverse transcription, and PCR full-scale amplification.
attributes.tag: GEO Accession
attributes.value: GSM4305750