title: BUF
design: The DNeasy Blood and Tissue Kit from Qiagen (Hilden, Germany) was used for DNA extraction. Samples were further QC'd and sequenced at Novogene (Beijing, China) following the standard Illumina protocols. Library preparation produced fragment libraries of ~350 bp insert length. Sequencing was done on Illumina HiSeqX-Ten to collect 150 bp paired-end data, aiming for an average depth of 25X per strain