title: Transcriptome of Lucania killifish
design: Sequences from cDNA from pooled gills, ovary, testes, eyes, and fin tissues from 10 pooled individuals (5 males and 5 females) from an Everglades population.  A pooled sample was created for each population containing equal amounts of RNA from all 10 individuals (concentrations determined via Nanodrop spectrophotometer, Thermo Fisher Scientific, Waltham, MA).  Construction of cDNA libraries and 100bp paired-end sequencing occurred at the Keck Center for Comparative and Functional Genomics at the University of Illinois (see supplementary information).  Size-selected libraries (250bp to 500bp) were diluted to 10nM, and fragments were sequenced from both ends on an Illumina HiSeq2000 using the TruSeq SBS sequencing kit version 2 (Illumina, San Diego, CA).  Fastq files were generated with Casava version 1.8 (Illumina, San Diego, CA).  Average quality scores were above 20 for all cycles.