title: RNA-seq of ADAR1KO HEK293T cells cultured from clone; Interferon beta treated; biological replicate clone 3
design: Libraries were prepared with the TruSeq Stranded Total RNA with Ribozero kit. ERCC RNA Spike-In Mix (Thermo Fisher Scientific) was added to each RNA sample. Two modifications were applied to the standard kit protocol in order to generate libraries with long fragments. First, RNA was fragmented at 94¡C for 2 minutes. Second, after ligating adaptors, the volume of AMPure XP Beads (Beckman Coulter) used was reduced to 60% of the suggested volume in the protocol.