title: Illumina HiSeq 2500 paired end sequencing; RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)
design: RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)
library_construction_protocol: RNA was isolated from 1-2 million cells using an RNeasy kit from Qiagen. GR18 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 24 hours with 1uM Dexamethasone or 0.1% ethanol as vehicle control Prior to reverse transcription, poly adenylated RNA was isolated using oligo d(T) beads. Sequencing libraries were prepared using the TruSeq RNA library Prep Kit (Illumina)
attributes.tag: Experimental Factor: compound
attributes.value: vehicle