title: GSM2975557: S2 cell stranded rRNA-depleted RNA-seq AGO1-dsRNA-treated low concentration replicate 1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: To 2.5 µg total RNA input we added ERCC spike-ins (External RNA Controls Consortium 2005) (AGO1 knockdown Experiment: mock and dsGFP: ERCC Mix-1; dsAGO1 low and high: ERCC Mix-2 (Life technologies #4456740 #4456739)), prior to rRNA depletion using RiboZero (Illumina #MRZG12324). 100ng rRNA-depleted RNA served as an input for RNA-seq libraries generated using the NEXTflex Rapid Directional qRNA-Seq kit (Bioo Scientific #5130) following manufacturer's descriptions. AGO1 RNA-seq libraries were sequenced single-end with 75nt cycles as a multiplexed pool on NextSeq500.
attributes.tag: GEO Accession
attributes.value: GSM2975557