title: Illumina HiSeq 2500 paired end sequencing; ChIP-seq for H3K27ac in the GR18 cell line treated for 1.5 h with 1 µM dexamethasone
design: ChIP-seq for H3K27ac in the GR18 cell line treated for 1.5 h with 1 µM dexamethasone
library_construction_protocol: ChIP-seq for H3K27ac was performed for cells were grown in DMEM containing 5% fetal bovine serum. GR18 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS Cells were treated for 90 minutes with 1uM Dexamethasone, cross-linked with 1% formaldehyde for 3 min and harvested. For H3K27ac ChIP-seq experiments, U2OS-GR cells were treated for 1.5 h with 1 µM dexamethasone, cross-linked with 1% formaldehyde for 3 min and harvested.  Chromatin was precipitated using 1µg of anti-H3K27ac antibody (Diagenode C15410196). Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep kit (NEB E7370) according to manufacturer's instructions and submitted for paired-end Illumina sequencing.
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: anti-H3K27ac (Diagenode)
attributes.tag: Experimental Factor: compound
attributes.value: dexamethasone
attributes.tag: Experimental Factor: dose
attributes.value: 1