title: Transcriptome of Lucania killifish
design: Sample of cDNA from pooled gills, eyes, fins, ovaries, and testes from 10 individuals (5 males and 5 females) from the Wakulla River, Florida, USA.  A pooled sample was created containing equal amounts of RNA from all 10 individuals (concentrations determined via Nanodrop spectrophotometer, Thermo Fisher Scientific, Waltham, MA).  Construction of cDNA libraries and 100bp paired-end sequencing occurred at the Keck Center for Comparative and Functional Genomics at the University of Illinois (see supplementary information).  Size-selected libraries (250bp to 500bp) were diluted to 10nM, and fragments were sequenced from both ends on an Illumina HiSeq2000 using the TruSeq SBS sequencing kit version 2 (Illumina, San Diego, CA).  Fastq files were generated with Casava version 1.8 (Illumina, San Diego, CA).  Average quality scores were above 20 for all cycles.