title: RNA-Seq of Colossoma macropomum: Juvenile: Exercise
design: For central nervous system (CNS) RNA extraction, we used the Dynabeads RNA DIRECT Micro Kit (Thermo Fisher Scientific) and followed the manufacturers suggested protocol for isolating RNA from tissues. For cDNA conversion, we used the Ion Total RNA-Seq Kit V2 kit (Life Technologies). Automated template preparation was done with the Ion Chef System (Thermo Fisher Scientific), following the manufacturers instructions. Sequencing of fragments was done with the Ion 540  Chip, and we used the Ion S5  System (Ion Torrent ) for reading the chip. Eight single end-read FASTQ files were generated that reference the eight biological replicas