title: RNA-Seq of Hosta leaf
design: A total RNAs from leaf tissue were isolated an RNeasy Plant Mini Kit and treated with an Ribo-Zero rRNA Removal kit. After quantification, 4 ng of RNA was used for library preparation using the TruSeq Stranded Total RNA Sample Preparation kit and an alternate fragmentation protocol was followed to obtain insert sizes of 280 bp as described in the manufacturers protocol. The obtained libraries were quantified using a Bioanalyzer DNA 1000 Kit and Ssofast EvaGreen supermix. A final concentration of 3 nM of the constructed library was sequenced on an Illumina NextSeq 500 sequencer using 2  150 bp chemistry.