GSM4594553: e13_5; Mus musculus; RNA-Seq
wo presumptive knee joints were isolated by transfemoral and transtibial division in a single 3 cm dish (Figure 1A) and incubated in digestion solution I (3 mg / mL collagenase D, DMEM high glucose culture medium, serum free) for 45 min at 37 °C, and then in digestion solution II (1 mg / mL collagenase D, DMEM high glucose culture medium, serum free) for 3 hrs (one embryo per dish) at 37 °C. During the period of incubation, the mice gender was identified by genotyping and only male samples were chosen for further processing. The tissues with medium were gently pipetted to disperse cell aggregates and filtered through 40 µm cell strainer, then centrifuged for 10 min at 400 g. The pellet was suspended with 0.4% BSA in PBS. Single-cell RNA-Seq libraries were prepared using GemCode Single-Cell 30 Gel Bead and Library Kit (now sold as P/N 120230, 120231, 120232, 10x Genomics) as indicated in Zheng et al 2017; PMID: 28091601) .
GEO Accession
GSM4594553