AVBF_MP5_1
We extracted total genomic DNA from two A. vulgare sisters using the Qiagen DNeasy Blood and Tissue kit, according to the protocol for animal tissues (3 h incubation in proteinase-K at 56C and 30 min RNAse treatment at 37C). These females are from our laboratory line BF2787 derived from wild animals sampled in Nice (France) in 1967.  One mate pair (~5kb inserts) sequencing library were prepared (one from each female) and sequenced as 50-100 bp