GSM5345754: HL60shHIF1p4; Homo sapiens; RNA-Seq
total RNA from a minimum of 1x10^6 up to 3x10^6 cells were isolated with QIAGEN RNeasy Plus Micro Kit according to the manufacturer's instructions. After isolation, total RNA was treated with DNAse I (Sigma) accordingly to the manufacturer's instructions. Libraries were generated with the TruSeq stranded mRNA protocol. This protocol allows unbiased 5'/3' library preparation starting from 100 ng of total RNA. Libraries were barcoded, pooled and sequenced on an Illumina Nova-Seq 6000 sequencing system.
GEO Accession
GSM5345754