GSM4943639: 5979_BRD; Bos taurus; RNA-Seq
Total RNA was extracted from Tempus tubes using a Preserved Blood RNA Purification Kit (Norgen Biotek Corp.,Thorlord ON, ), and the Nanodrop 1000 (Thermo Scientifc, Willmingotn, DE, USA) and 2200 RNA Screentape Tapestation System (Agilent Technologies Inc, Cedar Creek, TX) were used respectively to quantify and measure the RNA integrity Number for each sample. All samples had RIN values above 8,0 and were all used for library construction and RNA-seq was performed with samples in 4 separate pools of 12 samples each, with pool 4 having 11 samples. Total RNA was purified using poly-T oligo-attached magnetic beads and mRNA libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina Inc., San Diego, CA, USA).
GEO Accession
GSM4943639