GSM5888615: mcervix_D6; Mus musculus; RNA-Seq
Cervices were collected by dissection below the transition zone and all vaginal tissue was removed. Tissues were minced with a sterile razor. Minced cervices were dissociated with 10mg/ml cold protease (Sigma-Aldrich) with 1mg/ml DNase I (Roche) and 0.5 M EDTA (Fisher Scientific). After a 60 min incubation at 4ºC, cells were filters through a 40 µm cell strainer (BD Biosciences) and washed twice with fresh ice cold 1x PBS and then resuspended in 1x PBS +0.005% BSA. Library prepared following 10x v3 gene expression protocol.