GSM4944045: competition_amplicons_E2_T4; Bacillus subtilis; OTHER
8 mL of culture (from the pool of barcoded strains in competition) was collected at OD600=0.1 and mixed with 0.8 mL of 10:1 ethanol/phenol stop solution by rapid inversion. Cells were pelleted at 3000 rcf at 4∘C for 10 min and the supernatant decanted. The cell pellets were stored at -80∘C until DNA extraction. To extract genomic DNA (gDNA), we used the Promega Wizard gDNA extraction kit, following the manufacturer's protocol (scaling reagents volumes by 1/3×). Amplicons were generated by two rounds of nested PCRs to append UMIs, multiplexing indices, and sequencing adapters.
GEO Accession
GSM4944045