GSM4404479: P7_Testes_ChIP_Biol_1; Mus musculus; ChIP-Seq
Mixed background (129 sv/eV, MF1, CBA and C57Bl/6) Sox3 null mice were crossed onto the 129/SvJ genetic background and mice from the F6 generation were used. P7 testes were discected and mechanically dissociated into small fragments before fixation in 1% formaldehyde for 8 minutes at room temperature. Fixed tissue was lysed and sonicated (Bioruptor, Diagenode) for 15 minutes in 1-minute pulses on ice. SOX3 bound chromatin was immunoprecipitated by a goat polyclonal antibody raised against human SOX3 (R&D systems, AF2569). DNA was recovered by reversing crosslinks, and purified by PCR clean-up kit (QIAGEN). Three independent DNA libraries were produced with the KAPA HTP/LTP Library Preparation Kit as per manufacturer's instructions, and libraries were sequenced on the Illumina HiSeq producing 50 bp single end reads. Libraries were prepared according to KAPA HTP/LTP Library Preparation Kits instruction manual (KK8230)
GEO Accession
GSM4404479