GSM5346033: HF-cultured [H2c]; Mus musculus; RNA-Seq
RNA concentration and quality were assessed using the Nanodrop 2000 spectrophotometer (Thermo Scientific) and the RNA 6000 Pico assay (Agilent Technologies). Libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v2-Pico (Takara), according to the manufacturer's protocol. Libraries were pooled and sequenced 75 bp single-end on the NextSeq 500 (Illumina) using NextSeq High Output Kit v2.5 reagents (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM5346033