RNA-Seq of Mus musculus: adult male kidney
RNA was extracted from kidney with MoBio Labs Powerlyzer Ultraclean isolation kit then treated with DNAse-1 followed by a Zymo RNA Clean and Concentrator column clean-up. Ribosomal RNA depletion step then performed with KAPA Bioerase Kit and libraries then prepared with KAPA Hyperprep kit. All 30 sequenced then sequenced across two lines of 150bp PE Novaseq (one lane of S1, one of SP) at Vincent J. Coates Genomics Sequencing Center at UC Berkeley.