GSM3899772: CD14+CD11b+ cells from Patient 1; Homo sapiens; RNA-Seq
For amplification cells were heat-lysed at 65° C followed by cDNA synthesis using the CEL-Seq2 protocol using robotic liquid handling platforms Nanodrop II (GC Biotech) and Mosquito (TTP Labtech). After second strand cDNA synthesis, the barcoded material was pooled into libraries of 384 cells and amplified using IVT. Following amplification, the rest of the CEL-seq2 protocol was followed for preparation of the amplified cDNA library, using TruSeq small RNA primers (Illumina). The DNA library was paired-end sequenced on an Illumina Nextseq™500, high output, with a 1x75 bp Illumina kit