GSM4721984: Uninfected_1_150; Homo sapiens; OTHER
Genomic DNA of the sorted uninfected and infected cells and input cells was isolated with the Multisource Genomic DNA Miniprep Kit (Axygen Biosciences). The shRNA cassettes were amplified via PCR and separation from the genomic DNA was obtained via agarose gel electrophoresis. Samples were diluted to a final concentration of 2 ng/μl. Sequencing libraries were prepared for sequencing using standard Illumina protocols so that the read1 primer reads the first nucleotide of the siRNA.
GEO Accession
GSM4721984