GSM3301949: T47D, SMAD2 binding region after ActA stimulation, rep2; Homo sapiens; ChIP-Seq
Chromatin isolation, sonication and immunoprecipitation using anti-SMAD2 antibody (EP567Y, ab207451, Abcam) was performed essentially as described (Isogaya K et al., Cell Res, 2014; Morikawa M et al., Stem Cell Rep, 2016). The libraries were constructed as described (Murai et al, Cell Discov, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).
GEO Accession
GSM3301949