GSM5033989: Patient tumor sample_29_baseline; Homo sapiens; RNA-Seq
Snap frozen tumor biopsies were cut into smaller pieces on the cryostat. One section was used for haematoxylin and eosin staining to determine the tumor cell percentage in the biopsy, which was performed by an experienced pathologist. Total RNA was isolated using the Qiagen AllPrep DNA/RNA/miRNA universal kit. The NanoDrop (Thermo Fisher) was used to measure RNA concentration (ng/ul). Biopsies were sent for bulk RNA sequencing in case the tumor % was ≥ 30% and RNA concentration was not lower than 20 ng/ul. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5033989