GSM5577347: bir-2knockdown pathogen rep2; Caenorhabditis elegans; RNA-Seq
RNA was harvested using Trizol reagent. Total RNA isolation from the Trizol (Direct-zol, ZYMO) suspension was performed according to the manufacturer's instructions with DNAse treatment on the spin column. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. The library construction and sequencing were performed at the UNH Hubbard Genome Center core facility. RNA concentration and quality were assessed via Bioanalyzer nano. Ribosomal subtraction was performed using Illumina poly-A selection according to the manufacturer's instructions. Library construction was completed using KapaRNA Hyperprep v5.17 and the adapters were the KAPA Dual-Indexed Adapters (KK8722), and Illumina Tru-Seq RNA sequencing (2 X 150bp) paired-end sequencing was performed on Illumina HiSeq2000. All reagents were utilized following the manufacturer's protocols.
GEO Accession
GSM5577347