GSM4816021: ChHER.GABA.rna; Pan troglodytes; RNA-Seq
The dorsolateral prefrontal cortex (DLPFC) tissue specimens were sampled from frozen brains of macaque and chimpanzee subjects, in a region corresponding to human BA9. Nuclei of glutamatergic or MGE-derived GABAergic neurons were isolated by flow cytometry, using antibodies against neuronal nuclei (anti-NeuN) and MGE-derived GABAergic neurons (anti-SOX6). Total RNA was isolated from sorted nuclei, using PicoPure Kit (Thermo Fisher Scientific). RNA-seq libraries were constructed using SMARTer Stranded Total Pico-Input RNA-seq kit (Clontech) and 10 ng RNA from each sample. Sequencing was performed on an Illumina HiSeq 2500 instrument, using a paired-end 50 (PE50) protocol to an average of ∼50 million read pairs per sample.
GEO Accession
GSM4816021