GSM3900476: 5637_Mat1a-2_S11; Homo sapiens; RNA-Seq
Qiagen RLT buffer supplemented with beta-mercaptoethanol was used to isolate RNA directly 48 hours post transfection, for subsequent RNA isolation using Qiagen RNeasy spin column kit Libraries for sequencing were prepared using Illumina TruSeq RNA Preparation Low Sample kit version 2.0 Libraries were run on an Illumina NextSeq500 using High Output 75 cycles kit (2 x 36 cycles, single-end reads, single index)