GSM5885582: FourS20m [Set2d - 1]; Schizosaccharomyces pombe; RNA-Seq
Asynchronous growing log phase cells were collected and lysed using glassbeads and FastPrep-24 homogenizer (MP Biomedicals, Santa Ana, CA). Total RNA was extracted using TRIzol (Thermo Fisher Scientific, Waltham, MA), followed by twice extraction with phenol:chloroform:isoamyl alcohol (25:24:1) and once with chloroform. Samples were subsequently treated with DNase I to eliminate the DNA. RNA-seq was performed by BGI (Shenzhen, China) using an Illumina HiSeqTM 2000 platform (Illumina, San Diego, CA). cDNA