GSM3900111: ZC3H4-ChIPseq_LPS_45min_rep2; Mus musculus; ChIP-Seq
Ca. 200 mill. cells were pelleted and resuspended in PBS and fixed first with disuccinimidyl glutarate at 2mM final concentration for for 45 min at room temperature, followed by 1% formaldehyde fixation for 10 min. Nuclear lysates were prepared, sonicated to reach an average fragment length of 300 bp, whereafter protein-DNA complexes were immunoprecipitated with specific antibodies. DNA libraries were prepared for sequencing on the NextSeq as previously described (Ostuni, 2013).
GEO Accession
GSM3900111