Illumina HiSeq 4000 sequencing; RNA-seq in L3.6pl upon treatment with JQ1
RNA-seq in L3.6pl upon treatment with JQ1
Cells were treated for 24 hours before harvesting. Cells were treated for 24 hours before harvesting. RNA was isolated with QIAzol Lysis Reagent (Qiagen) added to the cells and centrifuged at full speed for 30 min. The aqueous phase was removed and mixed with equal volume of chloroform and centrifuged again for 20 minutes. The aqueous phase was then removed and the RNA was allowed to precipitate in isopropanol and incubated at -20°C overnight. Subsequently, RNA was washed twice with 70% ethanol and re-suspended in RNase free water.  The integrity of RNA was validated by gel electrophoresis and 500ng were used to make the libraries in triplicates for each condition. Libraries were made using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer's instructions.
Experimental Factor: compound
JQ1
Experimental Factor: dose
100