GSM4594504: cryo.dhBMEC_3; Homo sapiens; RNA-Seq
dhBMECs were subcultured at the end of the differentiaiton (fresh) or after one-year cryopreservation on six-well plates coated with 50 μg mL-1 human placental collagen IV and 25 μg mL-1 fibronectin from human plasma in endothelial media supplemented with 1% penicillin-streptomycin and 10 mM ROCK inhibitor Y27632.  After one hour, media was switched for an additional 24 hours in the same media, before switching to 24 hours in human endothelial serum free media supplemented with 1% penicillin-streptomycin and 10 mM ROCK inhibitor. At day 2 of subculture (for dhBMECs) or day 3 of culture for iPSCs, cells were washed with 1x PBS and lysed using RLT buffer supplemented with β-mercaptoethanol, before isolating RNA using a RNeasy Mini Kit (Qiagen) with DNase I digestion (Qiagen) RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM4594504