GSM5885623: 4T1 tumor tissue treated with DiI-labeled liposomes; Mus musculus; OTHER
ST library was acquired by several steps: cryo-sectioning, fixation, permeabilization, cDNA synthesis, and RNA sequencing. All the methods were carried out in the way  that 10x Genomics visium protocol recommended. The fresh tissue samples were embedded in OCT  compound (25608-930, VWR, USA). We prepared consecutive tissue slices were used for H&amp;E  staining, spatial transcriptomics library, and fluorescence imaging. The slices were acquired by thin  blades used in cryotome so that the fluorescence pattern affected by gene expression could be  explored thoroughly. The tissue section for ST were placed on Visum slides (both Visium Tissue  Optimization Slides, 1000193, 10X Genomics, USA and Visium Spatial Gene Expression Slides,  1000184, 10X Genomics). The fixation was performed under the recommended protocol using chilled  methanol. cDNA libraries were prepared and were sequenced on a NovaSeq 6000 System S1200  (Illumina, USA) at a sequencing depth up to 250M read-pairs. 10x Genomics visium protocol