GSM3900164: HeLa_4sU_siZc3h4_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol whereafter nascent 4sU-labelled RNA was extracted as described (Austenaa et al. 2015 ) Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) starting from ca. 100-300 ng of nascent 4sU-labelled RNA, without ribosomal depletion.