GSM3899604: hepg2_ctrl_d3_rep3; Homo sapiens; RNA-Seq
Infected and uninfected cells were harvested at 1- and 3-days post-infection. Total RNA was isolated from cells using the Qiagen RNeasy kit according to manufacturer's instructions. Libraries were prepared using Illumina's TruSeq stranded mRNA kit (Cat#20020594) following the manufacturer's recommendation for low sample protocol. A total of 500ng of RNA was end repaired, A-tailed and ligation controls were added to all samples.  Libraries were amplified using 15 PCR cycles and cleaned up using PCR Clean DX beads. Library sizes (~260bp) were confirmed on the Bioanalyzer using the DNA High Sensitivity kit.  Samples were multiplexed 12 per flow cell and performing 100PE reads. Libraries were sequenced on the HiSEq 4000 (Illumina).
GEO Accession
GSM3899604