Meta-transcriptome of Montipora capitata: MC-289_Field_289-3
M. capitata Field sample nubbins were collected on 30 May 2019. At the time of collection, each nubbin was transferred to a Whirl-pak under water, the water was drained from the Whirl-pak, and then flash-frozen in liquid nitrogen. The Qiagen AllPrep DNA/RNA/miRNA Universal Kit was used to isolate RNA, the TruSeq RNA Sample Preparation Kit v2 (Illumina) was used to prepare strand specific cDNA libraries. The protocol included poly-A selection; quality control was done using an Agilent Bioanalyer and a Thermo Fisher Qubit dsDNA BR (Broad Range) assay kit.