NextSeq 500 sequencing; 3' mRNA-seq of protrusions and cell bodies of BJ, PC-3M, RPE-1, U-87 and WM-266.4 cells
3' mRNA-seq of protrusions and cell bodies of BJ, PC-3M, RPE-1, U-87 and WM-266.4 cells
Protrusions going through 3 micrometers transwells for 2 h were shaved RNA was extracted with RTL buffer (quiagen) 3â€˜ mRNA-Seq Library Prep Kit FW
Experimental Factor: cell line
U-87 MG
Experimental Factor: sampling site
cell protrusions