GSM5718160: INP - w1118 control NanoDam Replicate 4; Drosophila melanogaster; OTHER
Genomic DNA extracted from 50 dissected brains per replicate according to Marshall, O et al., 2016, Nat Protoc For all DamID-samples, genomic DNA was extracted with the QiAmp DNA Micro kit, digested with DpnI, DamID-adaptors were ligated followed by DpnII-digestion and PCR-amplification (Marshall, O et al., 2016, Nat Protoc). For all DamID-libraries, the amplified products were sonicated to average size of 300 bp, DamID-adaptors removed by AlwI-digestion, end-repaired, A-tailed, followed by ligation of Sequencing-adaptors and PCR-amplification (see Marshall, O et al., 2016, Nat Protoc). Single cell RNAseq libraries were generated with the 10xGenomics 'SC3Pv2'-chemistry kit.
GEO Accession
GSM5718160