GSM3300868: Ranger7Pre_Stress; Homo sapiens; ncRNA-Seq
Micro RNAs were extracted from 200 ul of plasma samples using miRCURY RNA isolation kit, Biofluids (Exiqon) using manufacturer's guidelines.  Small RNA libraries were prepared using the TruSeq Small RNA Sample Preparation kit (Illumina, San Diego, CA, USA) following the manufacturer's instructions.  A fraction of ﬁve microliter of total RNA was used for library preparation as quantiﬁcation is below the detection limit. Five microliters of total RNA was ligated with 30 and 50 adaptors followed by reverse transcription with SuperScript II Reverse Transcriptase (Invitrogen) and unique indexes were introduced during PCR ampliﬁcation for 15 cycles. A multiplexed pool consisting of equimolar amounts of small RNA-derived libraries was prepared. The 140–150 nt region containing the microRNA fraction of the library was excised from a 6 % polyacrylamide gel after electrophoresis, ethanol-precipitated and quantiﬁed.  The samples were subsequently sequenced on the Illumina HiScan SQ instrument using 50 cycles plus 7 index sequencing cycles.
GEO Accession
GSM3300868