Illumina NovaSeq 6000 sequencing; Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages
Single-cell transcriptomics reveals early emergence of liver parenchymal and non-parenchymal cell lineages
Embryos were collected at noon of the experimentally-determined timepoint in ice-cold phosphate-buffered saline (PBS) under a Leica MZ6 dissecting scope.  Liver buds, including STM, were dissected in PBS with fine dissecting forceps, ensuring removal of cardiac, yolk sac, and non-hepatic endodermal tissues. Livers were washed in PBS, and incubated for five minutes at 37 degrees Celsius in 1ml PBS, 10ug/ml DNAse I, 4mM EDTA, and 2% fetal bovine serum (FBS) (PDEF) with dispase/collagenase (2.5uU/ml dispase, 0.0025% collagenase to dissociate tissues into single-cells. 10x Genomics Chromium Single Cell 3' Reagent Kit (v2 chemistry). As per 10x genomics manufacture's protocol
Experimental Factor: immunophenotype
unsorted
Experimental Factor: developmental stage
embryonic day 9.5