GSM4875374: H3K27ac_HCT_RIF1AID_KD_G1_ChIPseq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. After washing with high salt buffer, LiCl buffer, and TE, chromatin was eluted and reverse crosslinked. Purified DNA was ethanol precipitated followed by Illumina Truseq library preparation. Libraries for Illumina sequencing were constructed using the following standard protocol. Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3' ends of fragments using Klenow fragment (3' to 5' exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina barcoded primers using KAPA SYBR FAST qPCR Master Mix for 5~12 PCR cycles to obtain enough DNA for sequencing.
GEO Accession
GSM4875374