GSM3293634: 0_PAX7-B_bulk: Donor B - PAX7 negative myoblast population; Homo sapiens; RNA-Seq
Total RNA was isolated from human primary myoblasts using NucleoSpin® RNA/Protein Kit (Marcherey-Nagel, Germany) and treated with DNAse for genomic DNA removal. RNA quantity and purity were determined with a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, USA). RNA quality was evaluated using TapeStation (Agilent Technologies, USA). The RIN factor was >9 for all samples. cDNA libraries from rRNA depleted RNA for 75nt paired-end sequencing were prepared using the NEBNext® rRNA Depletion Kit and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina sequencing (NEB, USA). RNA sequencing was performed at Illumina MiSeq platform (Illumina, USA).
GEO Accession
GSM3293634