GSM5035429: wild type (BY4741) without poacid acid_3; Saccharomyces cerevisiae; RNA-Seq
Total RNA was isolated from exponentially growing cells by the “mechanical disruption protocol” using the RNeasy kit (Qiagen, Hilden, Germany), following the instructions of the manufacturer. RNA concentrations were determined by measuring absorbance at 260 nm. RNA purity and integrity were assessed using RNA Nano Labchips in an Agilent 2100B Bioanalyzer (Agilent Technologies, Palo Alto, CA) following the manufacturer's instructions. DNA libraries from depleted RNA were generated with the use of the "NEBNEXT Ultradirectional RNA Library Prep Kit" (New England Biolabs) and following the instructions of the manufacturer. Briefly, depleted RNA was fragmented and copied to double-stranded cDNA. Specific adapters for Illumina sequencing were attached to the cDNAs and the library was enriched by limited PCR. DNA fragments with 300-450 bp inserts were selected for massive sequencing.
GEO Accession
GSM5035429