GSM3900707: MC2; Danio rerio; RNA-Seq
RNA extraction was performed from PFA-fixed (4% PFA) and Tissue-Tek® O.C.T. compound (ProSciTech)-embedded heart cryosections (8μm). A scalpel was used to collect 12 sections of the ventricle per sample. Sections were transferred to a tube on ice containing 100μl of TRIzol® Reagent (15596018, Invitrogen) and then processed with Direct-zol™ RNA Microprep kit (Zymo/ Integrated Science, R2061). Ribosomal RNA was depleted from total RNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310, New England Biolabs) followed by library generation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) to select library fragment sizes of 200-400bp.
GEO Accession
GSM3900707