Actinobacterial diversity of an impacted peatland: sample G3
Triplicate samples were collected and for each sampling site, the following layers were combined: top sample (~5cm depth), middle sample (~25cm depth), and deep sample (~50cm depth). The sampling was repeated at a 30m interval, resulting in a total of nine samples (G1-G9). Metagenomic DNA was extracted using the Powersoil kit and subjected to 16S PCR. Amplicons generated were submitted for Illumina sequencing.