GSM4874507: B13_Treated larvae 0.12 ng EE2; Pimephales promelas; RNA-Seq
Larvae were removed from exposure vessels, placed into Eppendorf tubes together with a steel bead and frozen in liquid nitrogen.  MagMax lysing/binding buffer (100 μl) was added to each tube. Libraries were prepared from 250 ng RNA using the SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Greenland, NH) according to the supplied protocol.
GEO Accession
GSM4874507