GSM3899439: Mm_LT_Mx_rep2; Mus musculus; RNA-Seq
Bone marrow from from posterior limbs, anterior limbs and sternum were crushed. Recovered cells were stained and LT-HSCs were sorted in RLT lysis buffer. RNA was purified from 3-7,000 LT-HSCs taking advantage of the PicoPureTM RNA Isolation Kit (ThermoFisher) Libraries of template RNA molecules were prepared with 0.5 μg high quality input RNA using the SMARTseq protocol based on polyA-enrichment (Picelli et al. 2014)
GEO Accession
GSM3899439