GSM3297063: LCS4; Bos taurus; RNA-Seq
Approximately 100 mg of frozen tissue samples were ground to fine powder in a sterilized mortar and then liver and mamamry gland tissue samples were applied to isolate the total RNA using a mirVana total RNA Isolation Kit (Ambion, Carlsbad, CA, USA) while backfat tissue was applied to isolate total RNA using RNeasy Lipid Tissue Mini Kit (Qiagen, Germany) following the manufacturer's instructions. The RNA was quantified using the Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and checked for purity and integrity using the Agilent 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA). RNA with the ration of 28S/18S ranging from 1.7 to 2.4 and the RNA integrity number greater than 7.0 was used for RNA-Seq library construction. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3297063