GSM5034744: J8_1; Homo sapiens; RNA-Seq
Total RNA from 1 x 10^6 CB CD34+ cells was extracted using the RNeasy Plus Mini Kit (Qiagen). Briefly, CB CD34+ cells pellet were first lysed and homogenized. The lysate was passed through a gDNA Eliminator spin column, 70% ethanol was added to the flow-through, and the sample was applied to an RNeasy MinElute spin column. High quality RNA was eluted in 30 µl RNAase free water. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5034744