NextSeq 500 sequencing; RNA-seq comparison of fibro-adipogenic progenitors (FAPs) from wild type, mdx and cardiotoxin-injured mice
RNA-seq comparison of fibro-adipogenic progenitors (FAPs) from wild type, mdx and cardiotoxin-injured mice
For 3'-end RNAseq, FAPs were purified from wild type, mdx and ctx mice (n=4). Total RNA was extracted using Trizol according manufacture's recommendations. Libraries were prepared from 100 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH).
Experimental Factor: strain
C57BL/6J
Experimental Factor: injury
cardiotoxin