Illumina HiScanSQ paired end sequencing; RNA-seq of Sorghum bicolor with drought tolerant genotype grown under drought-stress or no-stress control environment
RNA-seq of Sorghum bicolor with drought tolerant genotype grown under drought-stress or no-stress control environment
Sorghum (Sorghum bicolor (L.) Moench) seeds of genotypes M35-1 (drought tolerant/DT) (Jogeswar, et al., 2006) was obtained from Directorate of Sorghum Research, Hyderabad, India. Seeds were grown at 28-32°C day/night temperature with 12/12 h light/dark period in the phytotron glass house. Plants were irrigated alternately with water and Hoagland’s solution at 3 days interval. Thirty days after sowing, drought stress was imposed by withholding irrigation to one set of plants (documented as treated with drought stressed; Sample 3 and 4) until the leaf relative water content reached to about 60-65%. The fully opened uppermost leaves from control and drought stressed seedlings were sampled and stored at -80°C. For RNA-Seq libraries, one microgram of total RNA was extracted with Trizol reagent (Invitrogen, USA) and mRNA libraries were produced using the TruSeq mRNA-Seq library kit (Illumina) according to manufacturer’s instructions.
Experimental Factor: treatment
Drought stress
LIBRARY_STRAND
second strand