NextSeq 500 sequencing; RNA-Seq to investigate the effect of small molecular compounds on the maturation capacity of monocyte-derived dendritic cells
RNA-Seq to investigate the effect of small molecular compounds on the maturation capacity of monocyte-derived dendritic cells
Monocyte-derived dendritic cells were collected by pipetting and used as input for isolation of RNA. RNA was isolated using Qiagen's miRNeasy Mini Kit with on-column DNA digestion according to the manufacturer's instructions. Library preparation was performed using the TruSeq Stranded mRNA Sample preparation kit from Illumina according to the manufacturer's instructions. All library preparation steps were conducted at Qiagen Genomic Services (Qiagen GmbH, Germany).
Experimental Factor: stimulus
6-BIO 0.5 micromolar
Experimental Factor: compound
ICG-001
Experimental Factor: dose
4