GSM5346045: Gata4 KD [Gata4-b]; Mus musculus; RNA-Seq
Total RNA was isolated from fibroblasts using the RNeasy Micro kit (Qiagen), according to manufacturers' protocols, including the optional DNase digest step. Sample concentration and quality were assessed using the Nanodrop 2000 spectrophotometer (Thermo Scientific) and the RNA 6000 Pico LabChip assay (Agilent Technologies). Libraries were constructed using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer's instructions. Libraries were pooled and sequenced 75 bp single-end on the NextSeq 500 (Illumina) using NextSeq High Output Kit v2 reagents (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM5346045