RNA-Seq of Cricetulus griseus: no expression vector
Fed-batch culture was performed in an ambr15 (Sartorius  Stedim)  using AZ proprietaryproduction medium and feeds, in a 14-day process. Each cell line was assessed in quadruplicatevessels. Cells were maintained with 50% dissolved O2, 35.5 C, and controlled pH. Glucoseconcentration   was   maintained   during   the   fed-batch   culture   and   measured   daily   with   aglucose/lactate analyser (YSI). Samples for transcriptomic analysis were taken on day 8,triplicate samples were taken from vessels with the highest viability. Aliquots of 5 x 106 cellswere spun down at 300 g for 5 minutes, the supernatant removed and the pellet snap frozen ondry ice and stored at -80 C. Cells were then resuspended in 200 L RNAlater, samples wereplaced at 4 C overnight and then stored at -80 C. Samples were thawed and RNA was extractedaccording to manufacturers instructions with the Qiagen RNeasy Plus Universal Mini Kit(Qiagen, Hilden DE). RNA integrity was checked with an Agilent RNA 6000 Nano kit (AgilentTechnologies, Santa Clara, CA, US) on an Agilent Bioanalyzer 2100 and samples with an RNAintegrity number (RIN) > 8 were sent for subsequent sequencing. Sequencing was performed onan Illumina platform at paired-end 2x150bp (Illumina HiSeq platform via a commercial serviceat GATC Biotech, Constance, Germany).