GSM3895657: Embryonic pancreas day E13.5, live population (Dapi -) [EP12-P4-1]; Mus musculus; RNA-Seq
Cells were sorted into 5µl Vapor-Lock (Qiagen) or mineral oil (Sigma) containing a droplet with primers and dNTPs. Cells were lysed at 65 degrees Celsius for 5 minutes. mRNA was then reverse transcribed by adding RT mix. RNA was processed by the SORT-seq protocol (Muraro et al. Cell Sys. 2016) Libraries were sequenced on a Illumina NextSeq500 using 75bp paired end sequencing.