GSM3900256: Taf13_C; Saccharomyces cerevisiae; OTHER
ChEC-seq was performed as previously described (Grünberg et al., 2016; PMID: 27797823; Zentner et al., 2015; PMID: 26490019) with following modifications. The final calcium concentration in the reaction mixture was 0.2 mM (2 mM in the original protocol) and MNase digestion was done for 5 min for all collected samples. Stop buffer was supplemented with D. melanogaster MNase-digested DNA (1 ng/ml stock concentration) in the amount calculated based on S. cerevisiae culture A600 measurement (volume = A600 x 8 ul). Sequencing libraries were prepared similarly as described (Warfield et al., 2017; PMID: 28918900) with several modifications. 1/6 vol of the final ChEC DNA sample was used as an input. Final adapter concentration during ligation was 6.5 nM. Following ligation, two-step cleanup was performed using 0.25X vol AMPure XP reagent in the first step and 1.1X vol in the second step. 18 cycles were used for library amplification.
GEO Accession
GSM3900256