GSM5171516: root, PI2; Nicotiana tabacum; RNA-Seq
Root samples were collected and frozen and ground in liquid nitrogen, and RNA was purified using Plant Rneasy mini kit of Qiagen. 100 ng total RNA samples was used for RNA-seq library construction using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA)  followed the protocol for long size insertions.
GEO Accession
GSM5171516