PacBio sequencing of Enterococcus faecium
DNA was fragmented by Megaruptor (Diagenode, 75 kb fragmentation mode) followed by purification using 0.45x magnetic beads. 5 g of fragmented DNA was introduced into a SMRTbell Template Prep Kit 1.0 following the manufacturer's protocol "20-kb-Template-Preparation-Using-BluePippin-Size-Selection".