GSM3898978: Knockout cold 3 [KC3]; Mus musculus; RNA-Seq
Total RNA was extracted from BAT tissue by Trizol (Invitrogen) using a dounce homogenizer (Wheaton). Total RNAs with an average RIN value of 7.5±0.16 were used for RNA-seq library preparation. mRNA-seq libraries were prepared as previously described (Takahashi et al., 2015)