Illumina NovaSeq 6000 sequencing; 3' UTR RNA-sequencing of human lung adenocarcinoma cell lines PC9 and A549 treated with RIG-I agonist IVT4/unspecific control IVT-GAC
3' UTR RNA-sequencing of human lung adenocarcinoma cell lines PC9 and A549 treated with RIG-I agonist IVT4/unspecific control IVT-GAC
Cells were treated with RIG-I agonist IVT4 or unspecific control IVT-GAC for 8h prior to harvest RNA was extrcted using Qiagen RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)
Experimental Factor: stimulus
unspecific control IVT-GAC