GSM5034786: CRISPR screen, Hoxb8 imortalised GM-CSF cell line, R26-CreERT2 Srsf2+/+ Day 4 rep 1 (S144); Mus musculus; OTHER
Genomic DNA was extracted from the cell pellets using the Gentra Puregene kit (Qiagen). The extracted DNA was quantified on a Nanodrop spectrophotometer. DNA libraries were generated by PCR amplification of the integrated sgRNA constructs (as described in https://portals.broadinstitute.org/gpp/public/resources/protocols). CRISPR screen. In total, 24 libraries were generated and sequenced on the Illumina HiSeq X Ten platform (150bp paired end reads) by Novogene (China).
GEO Accession
GSM5034786