GSM4875059: HMLER+shCTL#2; Homo sapiens; RNA-Seq
For gene expression analysis in HMLE and HMLER cells, total RNA was extracted from cells using the Qiagen RNeasy kit according to the manufacturer's instructions. For gene expression analysis in breast tumors, total RNA was extracted from frozen sections of breast tumor samples using the Nucleospin RNA set for Nucleozol kit according to the manufacturer's protocol (Macherey-Nagel). For HMLE and HMLER extracts, 25 ng for each of the two biological replicates per condition was used to generate libraries for whole-transcriptome analysis following High Throughput 3' Digital Gene Expression library preparation. For breast tumor extracts, libraries were prepared using a modified version of the Takara SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian kit.
GEO Accession
GSM4875059