RNA-seq of rattus norvegicus: rat dam liver
Liver were removed, flash frozen in liqid nitrogen, and RNA was extraxed using Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA, USA). Illumina TruSeq Sample Prep Kit was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA-seq data were trimmed then mapped to Rattus rattus genomic sequence (Rnor_5.0 release 79).