GSM5888866: MA-104 cells, Infected (36hpi), I2; Macaca mulatta; miRNA-Seq
The infected and control cell samples were collected from six-well plate in triplicate for each of the following time points: 0 hpi (just after adsorption time (90 min), 6 hpi, 12 hpi, 24 hpi, 36 hpi and 48 hpi. Three replicates of virus- and mock-infected cultures were prepared at each time point. Total RNA was purified from the cells using TRIzol (Ambion® (life technology, USA), 15596-026). Then strict quality control was carried out on the RNA samples. The quality control criteria included the following three aspects: Agarose gel electrophoresis: analyze the integrity of sample RNA and whether there is DNA contamination, nanodrop: preliminary quantification, detection of RNA concentration and purity (OD260/280 and OD260/230) and Agilent 2100 Bioanalyzer for accurate Detection of RNA integrity. The small RNA-SEQ technology process mainly consists of two parts: database building sequencing and bioinformatics analysis. After the qualified samples were detected, Small RNA Sample Pre-Kit was used to construct the small RNA library. Illumina deep sequencing was performed