GSM4874799: ADAM2; Mus musculus; RNA-Seq
Microglia were isolated from 18mo APP/PS1 (AD) and APP/PS1 Axl-/-Mertk-/- (ADAM) cortices, 2 pooled cortices per sample, 2 biological replicates. Briefly, cortices were digested using a combination of enzymatic digestion and mechanical dissociation followed by removal of debris on a Percoll gradient. Single cell preparations were subjected to FACS sorting based on CD45 positivity. Libraries were prepared according to Illumina's instructions on gene expression library constrcution (CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D) 10X Single-Cell RNA-Seq
GEO Accession
GSM4874799