GSM5184308: S13: CNS3KO; Mus musculus; OTHER
CD4+Foxp3-GFP+ nTreg cells were sorted from the spleen, skin draining LN, and mLN of individual WT GFP, CNS3, CNS0, and CNS3,0 mice at 16 days of age. Total RNA was extracted by TRIzol according to the manufacturer's instructions. cDNA was synthesized by SMARTScribe Reverse Transcriptase with primers targeting the constant regions of mouse TCRa (5'-AGTCAAAGTCGGTGAAC-3') or TCRb (5'-ATCTCTGCTTTTGATG-3') chains and template switch adapter (5'-AAGCAGUGGTAUCAACGCAGAGUNNNNUNNNNUNNNNUCTTrGrGrGrG-3'). TCRa and TCRb chains were further amplified by a two-step protocol. Sequencing adaptors were then added by Kapa Hyper Prep Kit (Kapa Biosystems). Samples were pooled and sequenced by Nova Seq with at least 10x coverage relative to the numbers of Treg cell used.
GEO Accession
GSM5184308