GSM5751809: rpoB_ptChIP_Col0_4_do_Col0_ChIP_1; Arabidopsis thaliana; ChIP-Seq
Five grams of rosette leaves were harvested and rinsed 3 times with ultra-pure water to eliminate soil debris and homogenized in chloroplast enrichment buffer (0.33 M Sorbitol, 30 mM HEPES-KOH (pH 7.5), 0.001% beta-mercaptoethanol) using a blender. The homogenate was filtered through two layers of miracloth, and the flow-through was centrifuged at 1500 g for 5 minutes at 4ºC. The pelleted chloroplasts were resuspended in 1 ml of chloroplast enrichment buffer ChIP-seq sequencing libraries were prepared as reported (Bowman, et al. 2013, BMC Genomics, 14:466).