GSM3294607: 37_1; Candida albicans; RNA-Seq
The bottom of the slide or dish was cut with a razor blade, removed, and placed in 1 × PBS. The cells were removed by sonication (power cycle 4, 20% pulse rate for 1 m) and collected by centrifugation (2300 × g for 5 min). Cells were re-suspended in 1ml Trizol and vortexed (4 cycle, 6m/s) with 0.45µM glass beads using a FastPrep-24 5g Homogenizer (MP Biomedicals, Santa Ana, CA). 200µl of chloroform was added, mixed vigorously for 15 sec and kept for 2 min at room temperature. Lysed cells were centrifuged at 21000 × g for 10 min at 4°C to separate the upper clear aqueous layer and mixed with 0.5 volume of 100% ethanol. Total RNA was purified using RNAeasy minikit from Qiagen (Hilden, Germany). The RNA samples were stored at -80°C. RNA libraries were prepared using the Illumina TruSeq RNA sample preparation kit.
GEO Accession
GSM3294607