GSM4875025: CrGFP control rep2; Ciona robusta; RNA-Seq
50-85 embryos were collected at Hotta stage 14 into RLT Plus buffer and stored at -80C until RNA purification.Half of the lysed sample was used for RNA purification with the Qiagen ALLPrep DNA/RNA Mini kit (Cat# 80204). Libraries were constructed with the NEBNext stranded mRNA library kit (Illumina) using standard protocols. Libraries were quality checked with an Agilent Tapestation and quantified by qPCR.
GEO Accession
GSM4875025