16S Microbiome of Pocillopora meandrina: coral frag
DNA was extracted using MoBio Powersoil Isolation Kit. PCR was performed on the V4 region of the 16S rRNA gene using the primer pair 515F and 806Rb. Amplicons were barcoding with barcoding primers with Nextera adapters, pooled in equal volumes, and purified with AMPureXP beads. Amplicon pools were paired-end sequenced on the Illumina MiSeq, 2x300 bp end version 3 chemistry at Oregon State University's Center for Genome Research and Biocomputing (CGRB) Core Laboratories.