GSM4875309: S1_fraction_HCT_RIF1KO_Highres_Repli-seq; Homo sapiens; OTHER
gDNA was isolated with SDS-PK buffer containing 0.2 mg/ml proteinase K for 2 hours at 56C followed by purification with 800 µl of Genomic Lysis Buffer from the Zymo Quick-DNA Microprep Kit according to manufacturers instructions DNA from G1 fraction was made into whole-genome sequencing library to normalize any bias during library preparation, sequencing and sequence read mapping. DNA from S1-S16 fractions were made into repli-seq library basically as described in (PMID: 29599440) but from 80K sorted cells instead of 20K sorted cells.
GEO Accession
GSM4875309