GSM4875348: HCT_RIF1KO_r2_HiC; Homo sapiens; Hi-C
For cell lysis, 550 μl lysis buffer (500 μl 10 mM Tris-HCl pH8.0, 10 mM NaCl, 0.2% Ige cal CA630; 50 μl protease inhibitors (Sigma, St. Louis, MO ) were added to one batch of cells (~ 25 million cells). Cells were incubated on ice for at least 15 minutes. Next, cells were lysed with a Dounce homogenizer by moving the pestle A up and down 10 times, incubating on ice for one minute followed by 10 more strokes with the pestle. The suspension was spun down for 5 minutes at 5000 rpm at RT. Cells were prepared and processed for Hi-C as previously described (PMID: 19815776)  using DpnII for digestion.
GEO Accession
GSM4875348