GSM3291781: ESPC2 +DOX; Homo sapiens; RNA-Seq
Total RNA was extracted from FA PSC-derived ESPCs +/-DOX in culture using the Pure RNA Isolation kit (Roche) according to manufacturer protocol. Isolated RNA were quantified (Qubit RNA assay kit) and quality controlled for degradation. RNA sequencing was conducted by the CCHMC DNA sequencing and Genotyping Core. RNA-seq libraries were prepared with the Illumina TruSeq RNA preparation kit. RNA-seq libraries were prepared with the Illumina TruSeq RNA preparation kit. The libraries were sequenced on an Illumina Hi-Seq 2500 sequencer to obtain 20 million of 75 bp paired-end stranded reads per sample.
GEO Accession
GSM3291781