GSM3899371: KO_Cyclic_Pressure; Mus musculus; RNA-Seq
BMDMs were plated at 1-2x106 cells per well on a flat bottom non-adherent 12-well plates. Following treatment, cells were washed once with PBS, and gently dislodged from the plate with PBS. Cells were pelleted and then processed using Qiagen RNeasy Mini Plus Kit (Qiagen) using the manufacturer's protocol. RNA quantity was assayed using a NanoDrop Microvolume Spectrophotometer (Thermo). mRNA for RNAseq analysis was purified using PolyA+ selection and processed by the Yale Center for Genome Analysis using standard methodology using Illumina TruSeq mRNA-Seq Sample Prep Kits, library quality and concentration assayed using a Bioanalyzer, RNA was sequenced on a Illumina HiSeq 2500 with 75bp pair-ended reads.
GEO Accession
GSM3899371