GSM5034098: Primary tumor (PT) cells; Mus musculus; RNA-Seq
Mammary glands of 'early stage' mice (EL), primary tumors from 'late stage' mice (PT) and lungs from 'early' and 'late stage' mice (eL and LL) were dissected and digested. After sorting, cancer cells (CD45- HER2+) were encapsulated using the 10X Chromium 3' v2 and chemistry kit according to manufacturer instructions. Sequencing, libraries were prepared according to manufacturer instructions. QC of cDNA and final libraries was performed by CyberGreen qPCR library quantification assay (KAPA). Samples were sequenced on an Illumina Nextseq 550 using the 75-cycle kit to a depth of 100 million reads per library.
GEO Accession
GSM5034098