GSM5774276: Ovarian granulosa cells-DOR_rep4; Homo sapiens; RNA-Seq
Total RNA was isolated and purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity of each sample were analysis of Agilent Bioanalyzer 2100 and RNA Nano1000 Assay Kit with RIN number >7.0. Approximately 5 ug of total RNA was used to deplete ribosomal RNA according to the manuscript of the Ribo-Zero™ rRNA Removal Kit (Illumina, San Diego, USA). RNA libraries were prepared for sequencing using standard Illumina protocols