GSM5184270: EMA305_Myh9_KO_5days; Mus musculus; RNA-Seq
For isolation of whole-intestinal epithelial cells, the mouse intestine was cut longitudinally and washed two to three times with ice-cold PBS, then cut into small pieces (3–7 mm long) and incubated for 30 min at 4 °C in PBS containing 30 mM EDTA and 1.5 mM Dithiothreitol on ice, and collecting suspended cells after gentle vortexing.Total RNA was isolated from mouse small intestinal epithelial cells from Ctrl and Myh9-KO mice at day 5 after administered with TAM using TRIzol Reagent (Life Technologies) according to the manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols.High-throughput sequencing was performed using the Illumina HiSeq 2000. Genes with fold changes of 1.4 were regarded as differentially expressed genes.
GEO Accession
GSM5184270