GSM5775589: L15_DMSO_M2_GW3965 reanalysis; Mus musculus; RNA-Seq
RNA was isolated from 500K BMDMs using the RNeasy Mini Kit (QIAGEN) with DNase treatment. 700 ng RNA was used for Illumina library construction. RNA amplification, cDNA generation, and adaptor ligation were performed using the KAPA mRNA HyperPrep Kit (Roche) following the manufacturer's instructions. Samples were pooled, diluted to 10 nM, and sequenced single-end on an Illumina HiSeq 4000 system (Illumina) to a depth of ±20 million reads with a length of 50 base pairs.