RNA-Seq of CD20-knockout cell line
RNA was isolated using RNeasy Mini Kit (Qiagen) and poly(A) mRNA selection was done using NEBNext Poly(A) mRNA Magnetic Isolation Module. Sequencing library was prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina using NEBNext Multiplex Oligos for Illumina (New England Biolabs). Sequencing was performed with the NextSeq 500/550 High Output v2 kit on Illumina NextSeq 500 sequencer using 75 cycles.