RNA-Seq of rats cecal contents: adult male rats
The V3-V4 hypervariable region of bacterial 16S rRNA gene was amplified by PCR using the primers 341F (CCTACGGGNGGCWGCAG) and 825R (GACTACHVGGGTATCTAATCC) containing specific barcode to tag each PCR product. The procedure of amplification and products purification had been described previously. Miseq platform (Illumina, San Diego, United States) was used for sequencing by Sangon Biotech Co., Ltd. (Shanghai, China) to generate pair-end reads.