GSM1854095: Holstein #3; Bos taurus; OTHER
gDNA was collected using the ArchivePure DNA Extraction kit (5 Prime, Gaithersburg, MD) Libraries were constructed based upon manufacturer's instructions for the Active Motif MethylCollector Ultra-seq kit. Briefly, genomic DNA was fragmented by sonication, repaired, and ligated with Illumina sequencing adapters. Enrichment for methylated DNA was conducted using the kit's provided nickel-coated dual protein (methyl-binding domain proteins) pulldown. Sequencing of the enriched library was then conducted following recommendations from Illumina.
GEO Accession
GSM1854095