GSM4875035: U0126 treatment 64cell rep3; Ciona robusta; RNA-Seq
300 embryos were collected at indicated Hotta developmental stage into RNA lysis buffer and stored at -80C until RNA purification.RNA was purified with the Zymogen Quick-RNA miniprep kit (R1054) and eluted in 50 ul elution buffer. Final purified RNA concentration was 52-73 ng/ul. Libraries were constructed with the NEBNext stranded mRNA library kit (Illumina) using standard protocols. Libraries were quality checked with an Agilent Tapestation and quantified by qPCR.
GEO Accession
GSM4875035