GSM5034797: CRISPR screen, Hoxb8 imortalised GM-CSF cell line, Srsf2P95H/+  Day 0 rep 1 (S81); Mus musculus; OTHER
Genomic DNA was extracted from the cell pellets using the Gentra Puregene kit (Qiagen). The extracted DNA was quantified on a Nanodrop spectrophotometer. DNA libraries were generated by PCR amplification of the integrated sgRNA constructs (as described in https://portals.broadinstitute.org/gpp/public/resources/protocols). CRISPR screen. In total, 24 libraries were generated and sequenced on the Illumina HiSeq X Ten platform (150bp paired end reads) by Novogene (China).
GEO Accession
GSM5034797