GSM5033007: fresh_mouse_sat_cell_rep3; Mus musculus; ATAC-seq
Chromatin accessibility studies were performed following the protocol described by Buenrostro and colleagues (Buenrostro et al., 2015). Except for freshly isolated human cells, which isolation yield ranged between 5,000 and 10,000 cells, all other samples consisted of 50,000 cells (freshly sorted mouse cells, cultured mouse and human cells). Briefly, cells were washed with 200 µl of cold PBS then resuspended in 100 µl of cold lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630), spun at 500 g for 10 minutes at 4°C and resuspended in 50 µl of the transposition reaction mix. Transposition occurred at 37°C for 30 minutes. Transposed DNA was purified using a Qiagen MinElute Kit and eluted in 10µl Elution Buffer. Primer extension followed by PCR amplification using Illumina-compatible adapter-barcodes and final library preparation were performed at the University of Minnesota Genomic Center.
GEO Accession
GSM5033007