NextSeq 500 paired end sequencing; RNA-seq of mouse epiblast stem cells (mEpiSCs), mouse embryonic stem cells (ESCs), and its derived primordial germ cell like cells (PGCLCs).
RNA-seq of mouse epiblast stem cells (mEpiSCs), mouse embryonic stem cells (ESCs), and its derived primordial germ cell like cells (PGCLCs).
Cells were dissociated with 0.25% Tripsin-EDTA (Gibco), washed with an equal volume of EpiSC mediume including 20% Knock-Out Serum Replacer (Invitrogen) and then collected by centrifugation.  Total RNAs were extracted from each cells using a NucleoSpin RNA Mini kit (MACHEREY-NAGEL).  Then, secondary RNA-purification was performed using NEB Next Poly A m-RNA magnetic bead selection kit (E7490), and each cDNA library was prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770).
Experimental Factor: genotype
wild type genotype
Experimental Factor: cell type
epiblast like cell
Experimental Factor: cell line
EpiLC