GSM3899745: AhR-/-_Stem cells_3 [AhR KO_3_SC]; Mus musculus; RNA-Seq
Colon crypts were isloated with 2mM EDTA wash. Crypts embedded in matrigel were cultured in stem cell enrichment media containing Wnt-3A, Noggin, Rspondin-1 and EGF. Resulting organoids were allowed to differentiate in Wnt-3A free media for 3 days. RNA was extracted from stem cells and differentiated organoids. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3899745