Station3 150mbsl
PCR reactions were performed with the general prokaryotic primers S-D-Arch-0159-a-S-15 and S-D-Bact-785-a-A-21 (Klindworth et al., 2013) using 454 GLX sequencing with a single-ended approach as described in Moore et al. (2015). The archaeal 16S rRNA gene amplicon sequences were analyzed by QIIME v1.9 (Caporaso et al., 2010). Raw sequences were demultiplexed and then quality filtered with a minimum quality score of 25, length in the range 250350, and allowing up to two mismatches in the barcode sequence.