GSM3899771: Rat88_E; Rattus norvegicus; RNA-Seq
Immediately after the stimulation period, the spinal cord was rapidly dissected and transversely sectioned into three 0.5 cm blocks encompassing the C4-C6, T7-T9 and L1-L3 spinal segments. The blocks were immediately placed in liquid nitrogen and stored at -80°C. RNA isolated from rat spinal cords was extracted using Trizol and purified using the QIAGEN (Venlo, Netherlands) RNeasy kit, by following the kit's standard protocol. Samples were analyzed with an Agilent Bioanalyzer (Santa Clara, CA). All analyzed samples passed minimum quality and quantity threshold required for RNA sequencing, including an RNA integrity score (RIN) above 7.0. Total RNA was prepped for sequencing with the Illumina TruSeq Stranded Total RNA kit with Ribo-Zero depletion according to manufacturer's protocols