GSM5576921: Control_MeRIP_Col0_20d_R2; Arabidopsis thaliana; RIP-Seq
Following control or treatment, seedlings were immediately placed on liquid N2 and ground using a mortar and pestle. RNA was extracted using Trizol according to manufacturers instructions. The total RNA from each sample was quantified using a NanoDrop ND-1000 instrument. The mRNAs were isolated from the total RNA samples and chemically fragmented to around 100-nt sizes. MeRIP was performed to enrich m6A methylated mRNA fragments with anti-N6-methyadenosine (m6A) antibody. The RNA-seq libraries for both the m6A immunoprecipitated RNAs and the input RNAs were prepared with a KAPA Stranded mRNA-seq Kit. The barcoded libraries were denatured to single-stranded DNAs, captured on Illumina flow cells, amplified in situ as sequencing clusters, and sequenced for 150 cycles on Illumina HiSeq 4000 system per the manufacturer,s instructions.
GEO Accession
GSM5576921