GSM3294640: NHSY_AP_3_4; Mus musculus; MBD-Seq
DNA samples were sheared with a Bioruptor Plus sonication device (Diagenode) to obtain average DNA size of 250 bp which was checked on a Bioanalyzer instrument (DNA 2100 kit, Agilent Genomics). Methylated DNA was isolated using the MethylMiner Methylated DNA Enrichment Kit (Invitrogen). This method uses the properties of the methyl-CpG-binding domain (MBD) to capture DNA fragments with CpG methylation. The total amount of captured DNA fraction was used for preparation of sequencing libraries (NEBNext Ultra DNA Library Prep Kit for Illumina, New England Biolabs) using the protocol described in (Nylander et al., Diabetes, 2016). Libraries were quality-controlled on a Bioanalyzer instrument (Agilent Technologies) and subjected to 100-bp single-end sequencing on HiSEq 2500 system (Illumina) at the Danish National High-Throughput DNA Sequencing Centre.
GEO Accession
GSM3294640