BCR-Seq of Mus musculus: draining lymph node samples
First-stranded cDNA was synthesized using 1 g total RNA and a SuperScript  First-Strand Synthesis System and mouse constant gene-specific reverse primers with a P7 sequence  (5'-TGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3'), and the UMI barcode. Synthesized cDNA was purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) according to the manufacturers instruction. Second-strand cDNA was synthesized using KAPA HiFi HotStart DNA polymerase (Kappa Bioscience, Oslo, Norway) and mouse variable gene-specific forward primers with a P5 sequence (5-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3) (98 for 4 min, 60 for 1 min, 72 for 5 min). Double-stranded cDNA was purified using AMPure XP beads and subjected to PCR amplification with KAPA HiFi HotStart DNA polymerase using two universal primers containing Illumina adapters and index sequences (95 for 3 min, 25 cycles of 95 for 30 sec, 65 for 30 sec, 72 for 1 min, and 72 for 5 min). Final products were purified using AMPure XP beads and submitted to a quality control procedure on TapeStation 2200 (Agilent Technologies, Santa Clara, CA, USA)