GSM3900114: Input_BMDM_SID100750; Mus musculus; ChIP-Seq
Ca. 100-150 mill. cells were fixed with 1% formaldehyde for 10 min. Nuclear lysates were prepared, sonicated to reach an average fragment length of 300 bp, whereafter protein-DNA complexes were immunoprecipitated with specific antibodies. DNA libraries of immunoprecipiated DNA were prepared for sequencing on the Illumina NextSeq as previously described (Ostuni, 2013).