GSM5643050: Sfb HepG2 r1; Homo sapiens; RNA-Seq
Total RNA was extracted from each sample using a RNeasy mini kit according to the manufacturer's instructions (QIAGEN, Germany). Libraries were prepared using RNA of cells treated either with the vehicle or with Sorafenib. Total RNA concentration and quality of the RNA were assessed with Qubit (Qubit™ DNA HS assay) and a 2100 Bioanalyzed Nano Chip (Agilent Technologies Genomics, USA), respectively. RNA Integrity Number (RIN) values were > 9 in all the RNA samples. Polyadenylated RNA was isolated from the total RNA using NEBNext Oligo d(T)25 beads (New England Biolabs, USA) according to the manu-facturer's instructions. Samples were normalised to an equivalent concentration of 67.3 ng/µl and prepared for RNA Ilumina Sequencing.
GEO Accession
GSM5643050