GSM5641733: SH-SY5Y SNCA-AS sample 2; Homo sapiens; RNA-Seq
Samples were homogenized and total RNA was isolated by Trizol® reagent (Life Science Technologies, Italy) following the manufacturer's specifications. RNAs were quantified using a Nanodrop ND-100 Spectrophotometer (Nanodrop Technologies, Wilmington, USA) and a 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit, Waldbronn, Germany); RNAs with a 260:280 ratio of ≥1.5 and an RNA integrity number of ≥8 were subjected to deep sequencing. SENSE Total RNA-Seq Library Prep Kit (Lexogen) using 500 ng total RNA and sequenced by Illumina NextSeq 550 sequencer.