GSM4594255: EV; Homo sapiens; RNA-Seq
As to RNA-seq, total RNA was extracted using TRIZOL Reagent (Life technologies) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100.Qualified total RNA was further purified by RNeasy micro kit and RNase-Free DNase Set. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample The ChIP was performed with an EZ-ChIP kit (Millipuro) following the manufacture's instruction as described above. In this experiment, the DNA fragments were sonicated between 100 bp to 500 bp. For Flag-MBD2a or Flag-MBD2c ChIP, 4.5×107 cells and Flag antibody (Sigma-Aldrich) were used. RNA-sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. ChIP-sequencing libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).
GEO Accession
GSM4594255