GSM4875054: Etr1>Bra/Foxa.a/Mnx rep1; Ciona robusta; RNA-Seq
300 embryos were collected at Hotta stage 19.5 into RNA lysis buffer and stored at -80C until RNA purification.RNA was purified with the Zymogen Quick-RNA miniprep kit (R1054) and eluted in 50 ul elution buffer. Final purified RNA concentration was 63-86 ng/ul. Libraries were constructed with the NEBNext stranded mRNA library kit (Illumina) using standard protocols. Libraries were quality checked with an Agilent Tapestation and quantified by qPCR.
GEO Accession
GSM4875054