RNA-seq of poorly-differentiated Caco-2 cells (13-HPODE-treated)
Total RNA was extracted from 13-HPODE-treated poorly-differentiated Caco-2 cells then cleaned using Turbo DNA-free RNA Cleanup kit. 500 ng of cleaned RNA was used to prepare the libraries for sequencing using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490))