RNA-Seq of Arabidopsis thaliana: roots
The 3RNAseq libraries were prepared from ~500ng of total RNA per sample using the Lexogen QuantSeq 3 mRNA-Seq Library Prep Kit FWD for Illumina (https://www.lexogen.com/quantseq-3mrna-sequencing/) with 13 PCR cycles.  The libraries were quantified on a Molecular Devices Spectra Max M2 plate reader (with the intercalating dye QuantiFluor) and pooled accordingly for maximum evenness. The pool was quantified by digital PCR and sequenced on an Illumina NextSeq500 sequencer, single-end 1x86bp, and de-multiplexed based upon six base i7 indices using Illumina bcl2fastq2 software (version 2.17; Illumina, Inc., San Diego, CA).