RNA-Seq of R.lauriocola: plate in vitro collection
For in vitro cultures utilized in the RNA-Seq analysis, a spore suspension of R. lauricola isolate PL571 was spread on the surface of a cellophane-covered PDA plate and incubated for seven days under ambient laboratory conditions (approximately 23C; 10 h light). Fungal tissues (spores and hyphae) from three separate biological replicates were harvested into 1.5ml microtubes, lyophilized overnight and stored at -80 until RNA extraction.