GSM5888928: D10; Homo sapiens; RNA-Seq
Cells were washed twice in PBS and dissociated with accutase (Stemcell Tech) at 37 C. After 1 h (30 min for D10 and D15 samples) cells were triturated with a wide bore pipet tip and passed through a 37μm reversible cell strainer. The single cell containing filtrate was collected in 4mL cold complete media. After washing cells were washed with PBS, counted and proceeded to library preparation snRNA-seq libraries were prepared with Chromium Next GEM Single Cell 3′ Kit v3.1 (10x Genomics). The libraries were sequenced at UCSF Center for Advanced Technology.