GSM4943586: AML Day 0 mTORC1 Low 3; Mus musculus; OTHER
Two million FM4-Venus cells were subjected to lentiviral infection of the Clontracer library plasmid with DsRed selection marker (Addgene (Cambridge, MA, USA)) with MOI < 10%. They were incubated for 3 days and sorted with FACS AriaII (BD). One million sorted cells were obtained. The sorted cells were expanded up to 1 billion by culturing for 10 days. One million cells were transplanted into each of the four groups of the four mice.  Four hundred thousand mVenus-high or low AML cells were sorted from the mouse bone marrow by sacrificing the mice immediately before (day 0) or at either of the 3 different time after chemotherapy (day 5, 7, and 14). Genomic DNA was extracted with the DNA/RNA mini kit (Qiagen) and sequencing libraries were constructed according to the protocol from Addgene.
GEO Accession
GSM4943586