GSM5885525: DMSO3.RNAseq.HCT116MCM2mAID; Homo sapiens; RNA-Seq
Total RNA from HCT116 cells were isolated using a lysis step based on guanidine thiocyanate (adapted from (Boom et al., 1990) and using magnetic beads (GE Healthcare, 65152105050450). mRNA sequencing libraries were prepared from 1 µg total RNA. mRNA sequencing libraries were prepared from 1 µg total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760). Paired-end sequencing was performed on Illumina NextSeq 500 (2 x 43 bp reads). Total 6 samples were multiplexed and sequenced on a NextSeq 500/550 High Output Kit v2.5 (75 Cycles) at MPIB NGS Core facility (www.biochem.mpg.de/7076201/NGS). BCL raw data were converted to FASTQ data and demultiplexed by bcl2fastq. mRNA-Seq