GSM5751894: INPUT, FLSHARP, Rescue, RepB; Mus musculus; ssRNA-seq
Cells were lysed in RIPA lysis buffer and bound to Halo-link resin to purify Halo-tagged SHARP variants. RNA from Halo captures were purifed and subjected to stranded RNA-seq library prepartion by the ligation of 3' adapter to RNA and cDNA prior to PCR amplification.