GSM5171214: PANC-1_Control siRNA_rep3; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol reagent (Thermo Fisher Scientific). The integrity of isolated RNA was verified using an Agilent 2100 Bioanalyzer. RNA samples with an RNA integrity number (RIN) greater than eight were normalized to 100 ng/μl before further analyses. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit setA according to the manufacturer's instructions and subsequently validated for an average size of about 330-340 bp using a 2100 Bioanalyzer and the Agilent DNA1000 kit. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit setA according to the manufacturer's instructions and subsequently validated for an average size of about 330-340 bp using a 2100 Bioanalyzer and the Agilent DNA1000 kit.
GEO Accession
GSM5171214