Fungal diversity of a developing peatland: sample 2b
Triplicate samples were collected and for each sampling site, the following layers were combined: top sample (~5cm depth), middle sample (~25cm depth), and deep sample (~50cm depth). The sampling was repeated at a 30m interval, resulting in a total of six samples (1a-2c). Metagenomic DNA was extracted using the Powersoil kit and subjected to ITS PCR. Amplicons generated were submitted for Illumina sequencing.