GSM5577268: bacteria only at t=30min; rep3; Vibrio cholerae O1 biovar El Tor; RNA-Seq
RNA was isolated using the mirVana Kit(Ambion, cat. no. AM1560) according to the manufacturer`s protocol. The rRNA was depleted using the Ribo-Zero rRNA Removal Kit from Illumina. The RNA integrity was checked on a Shimadzu MultiNA microchip electrophoresis system. cDNA libraries were prepared by adapter ligation, cDNA synthesis using the M-MLV reverse transcriptase and PCR amplification of the resulting cDNA. Quality of the prepared libraries was tested using the Shimadzu MultiNA microchip electrophoresis system.
GEO Accession
GSM5577268