GSM5888608: NSCs, input, rep2; Mus musculus; OTHER
ChIRP purified DNA was recovered from beads by Rnase A and RNase H treatment. Eluted DNA was treated with Proteinase K, extracted with Chloroform:Phenol: Isoamyl alcohol 25:24:1, and precipitated in 66% EtOH and 300 mM NaOAC. purified DNA was end-repaired, A-tailed, and ligated with sequencing adaptors. ChIP DNA