Illumina NovaSeq 6000 paired end sequencing; Metabolic alterations of liver Connexin-43 knockout in mice
Metabolic alterations of liver Connexin-43 knockout in mice
Trisol-based RNA extraction High-fat diet High-fat diet Trisol-based RNA extraction After the QC procedures, mRNA was enriched using oligo(dT) beads. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
Experimental Factor: genotype
AlbCre/Cx43 f/f