GSM5577305: hemB_Fe_6; Staphylococcus aureus subsp. aureus USA300; RNA-Seq
To extract RNA from frozen S. aureus and hemB S. aureus, cell pellets were washed with TE buffer (pH 8.0) and re-suspended in a Tris-HCl mixture with 1 mg/mL lysostaphin (pH 7.5) to lyse for 1 hour at 37°C. RNA was extracted using an AurumTM Total RNA Mini Kit (BioRAD #7326820). RNA samples were processed by the Microbial Genome Sequencing Center in Pittsburgh, PA. Ribosomal RNA (rRNA) was depleted (Qiagen FastSelect5S/16S/23S) and cDNA libraries were generated (Qiagen Total Stranded RNA). cDNA libraries were sequenced using NextSeq 550 using a 75bp x 8bp x 8bp setup.
GEO Accession
GSM5577305