Mi-seq A22
For the collection of microbes, three 50 mL seawater samples were combined from each distance prior to filtering on 47 mm 0.2-m nylon filters (Whatman, UK) in SwinLok filter holders (Sigma, Germany). Filters were stored in 100% Ethanol on ice until DNA extraction in the laboratory on the same day. DNA was extracted using the Qiagen All-prep-Kit (Qiagen, US) according to the manufacturers instructions. Recovered DNA was amplified by Fluidigm (Fluidigm Co., CA, USA) and sequenced using MiSeq 2x300 (Illumina, CA, USA) paired-end library at the Carver Biotechnology Center (University of Illinois-Urbana Champagne, USA).