GSM5577667: ΔmetE methionine 1, rep 1; Streptococcus pneumoniae; RNA-Seq
Total RNA was purified from cultures at the 6 hrs point. Total RNA was extracted using TIANGEN kit (TIANGEN, Beijing, China) and purified using QIAGEN kit (QIAGEN, Beijing, China). The ribosomal RNA was removed from total RNA (~1.5 µg) using poly-T oligo-attached magnetic beads. Fragmentation of mRNAs and sequencing libraries were generated using NEBNext® UltraTM RNA  Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. The cDNA molecules were ligated with the NEBNext Adaptor, and further processed for selected, purifed, and sequence amplification.
GEO Accession
GSM5577667