GSM4816012: Rh36.GABA.input; Macaca mulatta; ChIP-Seq
The dorsolateral prefrontal cortex (DLPFC) tissue specimens were sampled from frozen brains of macaque and chimpanzee subjects, in a region corresponding to human BA9. Nuclei of glutamatergic or MGE-derived GABAergic neurons were isolated by flow cytometry, using antibodies against neuronal nuclei (anti-NeuN) and MGE-derived GABAergic neurons (anti-SOX6). Histone modification ChIP-seq was performed using anti-H3K27ac antibodies, and a native MNase-digestion-based protocol. ChIP-seq libraries were constructed using NEBNext Ultra DNA Library Prep kit. Sequencing was performed on an Illumina HiSeq 2500 instrument, using a paired-end 50 (PE50) protocol to an average of ∼40 million read pairs per sample.
GEO Accession
GSM4816012