GSM3293582: Ppsal-WW-2; Pseudomonas pseudoalcaligenes; RNA-Seq
P. pseudoalcaligenes CECT5344 cells were suspended in 500 µl TRIsureTM (Bioline) and total RNA was isolated using the Direct-zolTM RNA Miniprep kit (Zymo Research) according to the instruction of the manufacturer. DNase incubation was carried out in the column with RNase-free DNase set and, when required, an additional post-column treatment was applied with DNase I (Ambion). Quality and quantity of the total RNAs were checked with Bioanalyzer (Agilent) and ND1000 spectrophotometer (Nanodrop 1000, Agilent Technologies-Wilmington, DE, USA). All samples showed an integrity number (RIN) higher than 6.9. Library preparation and sequencing were performed by STABvida, Portugal. After the integrity number (RIN) and concentration of RNAs were analyzed to ensure it is of sufficient integrity and quantity (>10 μg and 6.9, respectiely), cDNA was synthetized by using the Small RNA Library Preparation Kit (Bio Scientific). The resulting DNA library was sequenced in the Ilumina Hiseq 2500 platform using 100pb single-end sequencing reads. The corresponding FASTQ files were obtained as result.
GEO Accession
GSM3293582