Whole transcriptome sequencing of Homo sapiens : prostate RNA: Sample 435
Regions of tissue were excised from formalin-fixed paraffin-embedded tissues by microdissection and RNA was extracted using the RNeasy FFPE Tissue Kit. 1000ng of RNA were subjected to rRNA-reduction and strand-specific random-priming using the NEBNext Ultra II Directional RNA Library Prep Kit. Samples were sequenced on the Illumina platform.