GSM4874983: Mouse HSC-derived CAF from Panc02 liver metastasis model [RS031]; Mus musculus; RNA-Seq
HSC,CAF and whole tumor specimens were obtained perfusing and digesting the liver, followed by FACS sorting. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3′ v3 Reagent Kit (10x Genomics) according to manufacturer's instructions, 12 cycles of cDNA amplification and 12 cycles of library amplification were performed, and samples were sequenced on an Illumina HiSeq instrument (NovaSeq 6000 for AJ064) at the JP Sulzberger Columbia Genome Center.
GEO Accession
GSM4874983