GSM5643038: HepG2-NTCP, 1% Oxygen (1); Homo sapiens; RNA-Seq
Following treatment as described above, cells were lysed in RLT buffer and RNA extracted using Qiagen RNAeasy kit. RNAs were treated with on-column DNAse (Qiagen), and 2uG of material was used for the construction of sequencing libraries. Libraries were prepared for sequencing using standard Illumina protocols