GSM5577294: CA1 cNESCs P22/D10 6F rep1; Homo sapiens; RNA-Seq
Cell lysates were homogenized in QIAshredder columns (QIAGEN #79654) and total RNA was extracted with Rneasy mini columns (QIAGEN). RNA was DNase treated using TURBO DNase (ThermoFisher Scientific #AM2238). RNA quality was verified on a 2100 Bioanalyzer (Agilent Technologies) and polyadenylated RNA was enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB # E7490L) Libraries were generated using 100 ng of poly(A)+ RNA and Oxford Nanopore Technologies' Direct cDNA Sequencing Kit (ONT SQK-DCS109), with each sample barcoded with the Native Barcoding Expansion kits (ONT EXP-NBD104 and ONT EXP-NBD114), according to the manufacturer's specifications. Barcoded samples were pooled, loaded on a MinION sequencer on R9 Flow Cells (ONT FLO-MIN106D), and sequenced using the MinKNOW software v19.
GEO Accession
GSM5577294