GSM5681723: sample_040: Kidney from NVSel strain fish exposed to IHNV at timepoint 12 hrs post challenge; Oncorhynchus mykiss; RNA-Seq
Tissue samples were placed in RNAlater and stored at -80°C until usage. Total RNA was extracted with the Qiagen RNeasy Mini Kit per the manufacturer's recommendation. Total RNA was treated with Amplification Grade DNase I from Sigma-Aldrich and then precipitated prior to library construction. Library for RNA-Seq was prepared according to KAPA Stranded mRNA-Seq poly-A selected kit with 200-300bp insert size (KAPA Biosystems, Wilmington, MA) using 250 ng total RNAs as input. Final library quality and quantity was analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit 3.0 Fluorometer. The RNA integrity was checked by Agilent Bioanalyzer 2100 and samples with clean rRNA peaks were used for further experiments. RNA-Seq of 150 bp PE (paired end) reads were sequenced on Illumina HiSeq 4000 (Illumina Inc., San Diego, CA). 