RNA seq of Acinetobacter baumannii AB307-0294 logphase LB
Ribosome-depleted RNA samples were cleaved into fragments, the first strand was reverse transcribed to cDNA (Invitrogen SuperScript II reverse transcriptase), and then the second strand was synthesized (Illumina Second Strand Master Mix). After end repair and ligation, the products were enriched and purified to create a cDNA library for both AB307-0294 and AB307.70. The libraries were then quantified using Invitrogen Picogreen assay and Kapa Biosystems library quantification kit. Fragment Analyzer High Sensitivity NGS kit (Advance Analyticals) was used to confirm the quality and size of the cDNA libraries. The cDNA libraries were then normalized, pooled, and sequenced (Illumina HiSeq 2500).