GSM4874819: T1-preHSCs (Ter119-CD31+VCad+CD41lowcKit+); Mus musculus; RNA-Seq
Following sorting, each cell population was divided across two lanes on a 10X Genomics scRNA-Seq chip as independent technical replicates to encapsulate cells into droplets. scRNA-Seq libraries were  prepared per the 10X scRNA-Seq v2 protocol. Cells were loaded into 10X lanes at cell concentrations to maintain an estimated doublet rate below 5%. Final 10X libraries were assayed via an Agilent High Sensitivity dsDNA Bioanalyzer, normalized, pooled and shallow sequenced on a MiniSeq, identifying 10,000 high confidence cell barcodes in total across all conditions. 10X libraries were then renormalized per the distribution of reads/library from the MiniSeq run and deep sequenced on a NovaSeq S4 to a depth of 50,000 reads per cell barcode.
GEO Accession
GSM4874819