GSM4405369: P1Pro- (replicate 3); Nicotiana benthamiana; RNA-Seq
For each experimental condition, three biological replicates (each sample was a pool of ≥ 2 infected plants) were used for transcriptomic analysis (RNA-seq). Plant tissue was ground in a mortar in liquid nitrogen and total RNA was extracted with the FavorPrep plant total RNA mini kit (Favorgen), including on-column DNAse I treatment. Following a second DNAse I digestion, samples were purified by organic extraction and sodium acetate precipitation. Ribosomal RNA molecules were depleted by the RiboMinus plant kit (Invitrogen). Recovered RNA was quantified using a fluorescent RNA-binding dye (QuantiFluor RNA system, Promega), and analyzed in a Bioanalyzer with the RNA 6000 pico kit (Agilent). Libraries were prepared using the TruSeq stranded mRNA kit (Illumina), omitting the mRNA enrichment step, and were sequenced (2 x 100-nt paired-end reads) in an Illumina HiSeq 2000 at the Centro Nacional de Análisis Genómico (CNAG-CRG, Spain).
GEO Accession
GSM4405369