GSM5171296: T_LL2_PD1_DX400_2dose_8d_5; Mus musculus; RNA-Seq
For tumor tissue RNA isolation, organs were homogenized into RNA STAT-60 (Tel-Test Inc., Friendswood, TX) using a polytron homogenizer, then total RNA extracted according to the manufacturer's instructions. After isopropanol precipitation, total RNA was re-extracted with phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich, St. Louis, MO) using phase-lock light tubes (Thermo Fisher Scientific, Foster City, CA). RNA sequencing was performed using the Truseq stranded total RNA RiboZero library preparation kit (Catalog #: RS-122-2201) according to the manufacturer's instructions (Illumina, San Diego, CA). The resulting cDNA libraries were sequenced on Illumina HiSeqTM 4000 using a 50 base paired-end run.
GEO Accession
GSM5171296