GSM5346168: Brain Nuclear 5381; Homo sapiens; RNA-Seq
RNA was extracted by a hot Trizol extraction method. Nuclei were washed in PBS and resuspended in Trizol at 65°C and incubated on a shaker at 1,300 rpm for 15 min. RNA was enriched using a guanidinium HCl buffer and silica-coated magnetic beads with a DNAse I treatment step. Libraries were prepared using the TruSeq Stranded mRNA library prep kit. Standard Illumina protocols
GEO Accession
GSM5346168