GSM5751959: D3-IS-mice2-left kidney tissue; Mus musculus; RNA-Seq
Total RNAs were extracted from samples using the RNeasyMini Kit (Qiagen, Hilden, Germany). RNA quality was assessed by using NanoDrop (Thermo Fisher Scientific, Waltham, MA) and Qubit Fluorometer (Invitrogen). High-quality RNA samples (28 S/18 S = 2.0−2.2, RIN > 9.0) were used to construct sequencing libraries following a standardized procedure on the Illumina HiSeq 2000 Sequencing System.