GSM4875419: scRepliseq_HAP1_WT_part1; Homo sapiens; OTHER
single cells were sorted from five evenly spaced windows throughout S phase directly into individual wells of a 96 well plate containing single cell lysis buffer and lysed at 50°C for 1 hour followed by 4 minutes at 99°C Whole genome amplification (WGA) was performed as previously described (PMID: 22555242) and unique barcodes were added to each individual WGA product followed by purification and pooling for NEBNext Ultra DNA Library preparation.
GEO Accession
GSM4875419