GSM5752948: NSSCC-IgG-IP miR-449 Rep1 [Ctrl_IgG_miR449]; Homo sapiens; RIP-Seq
The cell lysates were centrifuged at 16,000g for 10 minutes at 4°C and incubated for 180 minutes with Protein G Sepharose beads (Thermo Fisher Scientific) coupled with anti-Ago antibody (10 μg, Invitrogen) or rat IgG isotype control (10 µg, Millipore). cDNA libraries were generated using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Clontech)