DNA-seq Urosperum dalechampii x Urospermum picroides
The quantity of the extracted DNA was measured with a Qubit 3.0 Fluorometer (Thermo Scientific). Paired-end shotgun libraries (TruSeq DNA PCR-free, 2x150 bp) with an average insert size of 450 bp were prepared and sequenced by Macrogen Inc. (Seoul, Korea) on a NovaSeq 6000 Illumina sequencing system (Illumina, San Diego, CA, USA)