GSM5732714: Retina Elovl2C234W rep2; Mus musculus; RNA-Seq
Fresh bulk mouse retina tissue were collected and homogenized thoroughly in TRIzol Reagent (15596026, Invitrogen) followed by RNA isolation.. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. The poly-adenylated (poly-A) transcripts from the isolated total RNA sample were enriched and performed strand specific mRNA-Seq TruSeq libraries preparation for sequencing using standard Illumina protocols