GSM5263990: CX5_1: Single cell 5' gene expression of fresh cevix biopsy/whole cell lysate/subject 5; Homo sapiens; RNA-Seq
Human ectocervix biopsies were processed into single cell suspension for 10x singel cell immune profiling (5' gene expression + TCR), usually within the four hours after biopsies were collected from the UW virus research clinics (VRC). The process of cervix biopsies were a combination of collagenase, DNase and mechanical agitation (details in the associated manuscript). Cell suspensions were loaded according to 10x Genomics Manufacturer's instructions for Chip A to generate Gel Bead-In-Emulsion (GEMS, 10x genomics) using barcoded gel beads. RNA containing GEMS underwent barcoded cDNA synthesis. Subsequent library construction followed 10x Genomics 5' Single Cell Immune Profiling Manufacturer's Instructions. Libraries were constructed using the Chromium Next GEM Single Cell 5' Library & Gel Beads Kit v1.1 with additional Chromium Single Cell V(D)J Enrichment Kit, Human T Cells kit for TCR Libraries.
GEO Accession
GSM5263990