GSM5171446: Single Cell ATAC-seq scATAC_GM_LargeScreen_Rep3; Homo sapiens; ATAC-seq
Up to 500,000 FACS-isolated immortalized cell line cells were processed for scATAC-seq. Cells were pelleted in a fixed-angle rotor at 300 RCF for 5 minutes at 4°C. The supernatant was removed, and the pellet was resuspended in 100 uL of ice-cold lysis buffer (RSB containing 0.1% Tween-20, 0.1% NP-40, and 0.01% digitonin) and incubated on ice for 3-5 minutes. To dilute lysis, 1 mL of chilled RSB-T was added to each tube and the cells were pelleted as before. The supernatant was removed and the pelleted nuclei were resuspended in Diluted Nuclei Buffer (10x Genomics). The nuclei stock concentration was determined for each cell line using Trypan Blue and a total of 5,000 nuclei from each cell line were pooled together and loaded into the 10x Genomics scATAC-seq (v1) transposition reaction. scATAC-seq library preparation was performed as recommended by the manufacturer (10x Genomics scATAC-seq (v1)).
GEO Accession
GSM5171446