RNA-seq of Icthyomyzon fossor: differentiated female
Total RNA  from gonadal tissue stored at -80C was extracted using the Qiagen RNeasy mini kit and genomic DNA removed using the RNase-free DNase kit (Qiagen). Messenger RNA was isolated from total RNA using a poly-A or poly-T step with the Illumina TruSeq DNA or Epicentre ScriptSeq kit. Sequencing was performed using 100 bp (TruSeq) or 75bp (ScriptSeq) paired-end sequencing on an illumina Hi-Seq 2000.