GSM3900884: s06_WT_RNAseq_biorep2; Arabidopsis thaliana; RNA-Seq
PlaNET-Seq: Nuclei were isolated from 3 grams of seedlings. Chromatin was solubilized by DNase I treatment. Nascent RNA fraction was enriched by immunoprecipitation of FLAG-tagged RNAPII elongation complexes using anti-FLAG antibody (F3165, Sigma-Aldrich) coupled to Dynabeads Protein G. Finally, RNAPII complexes were eluted from Dynabeads by 3xFLAG peptide, and the nascent RNA was purified using miRNeasy kit (QIAgen). RNA-Seq: total RNA was isolated from seedlings using QIAgen Plant RNeasy kit. PlaNET-Seq: NGS libraries were constructed from nascent RNA using Small RNA-Seq Kit v3 (Bioo Scientific). The original protocol was modified to incorporate alkaline RNA fragmentation step after 3' adapter ligation. RNA-Seq: polyA-enriched libraries were constructed using Illumina TruSeq RNA Sample Prep Kit v2.
GEO Accession
GSM3900884