RNA-seq of PheRS-m mutants in Drosophila melanogaster
The age of adult fly within 7 days were collected and quick-freezed in liquid nitrogen for 5 mins. Then squeezed frozen flies in TRIzol reagent (Invitrogen) to extracted the whole-body RNA of adult fly. Three genotype flies were collected including wild type flies as control and PheRS-mC19X/Cyo-GFP heterozygous flies, PheRS-mD127Y/D127Y homozygous flies as experimental groups. Each genotype contains more than 15 flies. The sample library products were sequenced using an Illumina Novaseq 6000 (Gene Denovo Biotechnology Corporation, Guangzhou, China)