GSM4875216: mES_Brd2_ChIA-PET_rep1; Mus musculus; OTHER
We modified ChIA-PET protocol based on previously described version (Ji et al., 2016). Briefly, the nuclei were sonicated and incubated with specific antibody and it was the same as the procedure described in the ChIP-Seq, and then, after proximal ligation of ChIP-DNA fragments, tagmentation of ligated products and purification, DNA were amplificated by PCR. ChIA-PET library was generated by Illumina HiSeq 2000.
GEO Accession
GSM4875216