GSM5731012: Nfr Liver Old Het Fasted F smp22; Nothobranchius furzeri; RNA-Seq
Samples were disrupted by bead beating in 300μl of TriZol (Sigma) and a single 3mm metal bead (Eldan, BL6693003000) using TissueLyzer LT (QIAGEN, #85600) with a dedicated adaptor (QIAGEN, #69980). Beating was performed twice at 50Hz for 2 minutes. RNA extraction was performed with Direct-zol RNA Purification Kits (Zymo). RNA concentration and quality were determined by using an Agilent 2100 bioanalyser (Agilent Technologies). Library preparation was performed using KAPA mRNA HyperPrep Kit (ROCHE-08105936001) according to the recommended protocols.  Library concentrations were measured by Qubit (dsDNA HS, Q32854), and quality was measured by Tape Station (HS, 5067-5584). Libraries were sequenced by NextSeq 500 high output kit V2, 75 cycle single-end (Illumina, 20024906) using a NextSeq 500 machine (Illumina) with ~30 million reads per sample.
GEO Accession
GSM5731012