GSM5263980: SqY2; Stenocereus queretaroensis; RNA-Seq
0.1 g of SqY1 and SqR1 were used for RNA extraction with the kit PureLink™ RNA Mini Kit (Invitrogen) with some modifications: we used 1 mL of TRIzol™ Reagent buffer for lysis, homogenization was carried with glass 0.5 millimeters glass beads, 500 mL of isopropanol was added to homogenate and then was passed through kit columns according to the manufacturing procedure. RNA was eluted with 50 mL of water 1 µg of total RNA per sample was used as input material for each sequencing library by using poly-T oligo-attached magnetic beads. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations, index codes were added to attribute sequences to each sample, and library quality was assessed on the Agilent Bioanalyzer 2100 system.RNA-sequencing and de novo assembly. Pair-end sequencing configuration (150PE x 40M raw reads) were performed on a Novaseq 6000 sequencer.
GEO Accession
GSM5263980