GSM4875262: Sorted CAF1 subset from murine PyMT tumour S5; Mus musculus; RNA-Seq
Tissues were minced using scalpels, and then single cells were liberated by incubation for 60 mins at 37°C with 1 mg/ml Collagenase I from Clostridium Histolyticum (Sigma-Aldrich) and 0.1 mg/ml Deoxyribonuclease I (AppliChem) in RPMI (Gibco). Released cells were then passed through a 70 μm cell strainer prior to staining for flow cytometry FACs sorting. RNA was extracted with the RNeasy Plus Micro kit (Qiagen) as per the manufacturers' protocol, in addition to on-column DNase digestions specified by the manufacturer (Qiagen). cDNA was generated and amplified using the SMARTseq v4 Ultra Low Input RNA Kit (Clontech) and libraries were generated with the Ovation Ultralow Library System V2 (NuGEN)
GEO Accession
GSM4875262