GSM4874826: APP/PS1 group rep3; Mus musculus; RNA-Seq
Brain endothelial cells were isolated by FACS (CD31+CD45-) from brain homogenates. 20000 cells were collected and RNA was extracted from isolated cerebral ECs using Quick-RNA MiniPrep kit (Zymo Research) with the in-column DNase treatment. Each library was prepared using the Smart-seq2 protocol with a tagged oligo (dT) primer to make first strand cDNA from 1.5ng total RNA input. Double-stranded cDNA was generated for library construction with 12 cycles of PCR and 0.075 ng of cDNA was used as input in a ¼ volume of standard NexteraXT (Illumina) reaction. Libraries were sequenced on an Illumina NextSeq500 instrument with 50 bases for read1 and 25 bases read2.
GEO Accession
GSM4874826