GSM3301764: s7C5F397: DN3_NMe_WT-3; Mus musculus; RNA-Seq
Total RNA was extracted using Qiagen RNeasy Micro Kit (cat number 74004). GENEWIZ (South Plainfield, NJ) performed the RNA library preparations and next generation sequencing. The SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA) as per manufacturer's protocol. Illumina Nextera XT library was used for library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed by limited-cycle PCR to enrich and add index to the cDNA fragments. Final libraries were analyzed on the Agilent TapeStation for library sizing, and quantified using the Qubit dsDNA HS Assay Kit and by quantitative PCR using KAPA Library Quantification Kit. Sequencing was performed on an Illumina HiSeq platform using a 2x150 Paired End (PE) configuration to generate a data output of approximately 58 million reads per sample.
GEO Accession
GSM3301764