GSM3899375: epidermis_KO_1; Mus musculus; RNA-Seq
Skin and tongue were collected from newborn mice. Tissues were frozen on dry ice and stored at -80 °C before further processing. Epidermis was manually separated from dermis after incubation for 5 min at 60 °C in 5 mM EDTA. Tissues were homogenized in Trizol reagent, and RNA was extracted according to the manufacturer's protocol.  Double-stranded cDNA was prepared using the SMARTer V4 Ultra Low Input Kit (Clontech, Mountain View, CA).  Libraries were prepared using a TruSeq v2 kit (Illumina, San Diego, CA), individually barcoded, pooled to a 2 nM final concentration, and sequenced on a NextSeq500 sequencer (Illumina) using 37 x 37 paired-end mode.