GSM5683073: QuantSeq_OPM2_2hours_DMSO_Replicate2; Homo sapiens; RNA-Seq
Cells were collected and washed once with ice-cold PBS prior to resuspension in TRIzol™ (ThermoFisher Scientific, 15596026). RNA was isolated using the Direct-zol RNA MiniPrep kit (Zymo Research, R2052) according to the manufacturers instructions. Sequencing libraries were prepared from 500ng RNA using the QuantSeq 3'-mRNA Seq Library Prep Kit for Ilumina (Lexogen, Vienna, Austria). Libraries were sequenced on the Illumina NextSeq 500 to obtain 75 b.p. single-end reads.
GEO Accession
GSM5683073