RNA-seq of Oculina patagonica
For Total RNA isolation, coral fragments from their natural habitat were snap-frozen in liquid nitrogen and ground into powder using a pestle and mortar and transfer into TRIzol Reagent (Invitrogen, USA). further homogenization was done using QIAshredder (Qiagen, Germany) and RNA extraction was completed using the PureLink RNA Mini Kit (Invitrogen, USA). For mRNA sequencing, paired-end reads for Illumina sequencing were generated from oligo-dT selected total RNA using the Illumina TruSeq RNA Sample Prep Kit (Illumina, USA) according to manufacturer's instructions. A total of 116,905,093 paired-end read pairs were sequenced on the HiSeq 2500 platform (Illumina, USA).