Illumina HiSeq 4000 sequencing; Ezh2 chip-seq of Th1 and Th2 cells during the first 48 hours of cell differentiation.
Ezh2 chip-seq of Th1 and Th2 cells during the first 48 hours of cell differentiation.
Spleen and lymph nodes lymphocytes were collected and differeniated into Th1 and Th2 cells for 24 hours Cell were fixed, lysed and sonicated for chip assay, reverse-crosslinking was conducted and DNA was purified using Ampure magnetic beads. UltraDNA nebnext kit' conducted by genomic center.
Experimental Factor: immunoprecipitate
anti-EZH2
Experimental Factor: cell type
naive T cell