Illumina HiSeq 2000 paired end sequencing; Transcriptome analysis of HeLa cells upon deprivation of methionine and cysteine (Met/Cys)
Transcriptome analysis of HeLa cells upon deprivation of methionine and cysteine (Met/Cys)
Cells were grown in regular DMEM or in DMEM lacking Met/Cys for different time points (6-30-120 hours for regular DMEM or 6-15-30-72-120 hours for medium w/o Met/Cys) .  Cells were trypsinized and collected by centrifugation, then immediately frozen at -80°C.  Total RNA was prepared using Trizol (Sigma), to preserve transcripts of both small and long sizes, and DNase treated to avoid contamination of genomic DNA. Ovation RNA-Seq System V2
Experimental Factor: stimulus
none
Experimental Factor: time
120