GSM3900384: Eomes input_rep 2; Mus musculus; ChIP-Seq
Chromatin immunoprecipiation was performed using the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher) as per manufacturer's instructions with a ChIP-grade anti-Eomes antibody from Abcam (ab23345). Non - immunoprecipitated DNA was used as an input control. ChIP DNA was blunt ended, had addition of “A” base to 3' end, and had sequencing adapters ligated to the ends. The fragments were size selected to 200-600 base pairs, and underwent amplification for 15 cycles with primers incorporating p5 and p7 sequences and a unique index tag for multiplexing.
GEO Accession
GSM3900384