GSM5183417: PD-1 KO mice control 1; Mus musculus; RNA-Seq
Lung tissues were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. 500 ng total RNA was used for rRNA-depletion (NEB, E6310), followed by directional library synthesis (NEB, E7420). Poly-A purification: Poly-T column kit (NEB, E7490), followed by NEB kit (E7420). Illumina NextSeq500 was used for RNA-seq. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5183417