Illumina HiSeq 4000 sequencing; Chip-seq of EZH2 and VAV1 in 24hr differentiated Th1 and Th2 cells with or without EZH2 or Actin polymerization inhibitors
Chip-seq of EZH2 and VAV1 in 24hr differentiated Th1 and Th2 cells with or without EZH2 or Actin polymerization inhibitors
Spleen and lymphnodes lymphocytes were collected and differeniated into Th1 and Th2 cells for 24 hours Cell were fixed, lysed and sonicated for chip assay, reverse-crosslinking was conducted and DNA was purified using Ampure magnetic beads. UltraDNA nebnext kit' conducted by genomic center.
Experimental Factor: immunoprecipitate
anti-VAV1
Experimental Factor: cell type
T-helper 1 cell