NGS of the gut of Tenebrio molitor: imago
The sequencing was conducted on the MiSeq sequencer, in technology pai red-end (PE), 2x250nt, using a set of MiSeq Reagent Kit v2 reagents, according to the manufacturers instructions (Illumina). Automatic data analysis was carried out on the MiSeq apparatus using the MISeq Reporter software (MSR) v2.6, 16S Metagenomics protocol. The analysis consisted of three stages: automatic demultiplexing of samples, generating fastq files containing raw reads and classification of paired-out readings in individual taxonomic categories. The 16S Metagenomics Protocol provides classification of readings to the species level basing on Greengenes v13_5  reference sequences database, modified by Illumina. Preparation of the reference sequence database included: filtering out sequences with a length of less than 1250 base pairs; filtering out sequences containing more than 50 degenerate pairs and filtering of incomplete classified sequences.