GSM5751927: ATAC-seq, Sen, 3; Mus musculus; ATAC-seq
The sensitive and resistant organoids were harvested at 3 days after culture, and were gently digested into single cell suspension using TrypLE. 20,000 cells were used for ATAC-seq. Library preparation was performed as previously described (2013 Buenrostro). Briefly, cells were washed lysed using lysis buffer followed by transposase reaction  (Novoprotein). Transposed DNA was purified using MinElute PCR Purification kit (Qiagen) and amplified using NEBNext High-Fidelity 2X PCR Master Mix(NEB) followed by purified using MinElute PCR Purification kit (Qiagen).