GSM5681129: AC2-6_Renal Medulla; Macaca fascicularis; RNA-Seq
Total RNA was extracted from 27 snap-froze tissues with RNeasy Micro/Mini Kit (QIAGEN, Germany, Cat. #74004/#74106), according to the manufacturer's instructions. The quality of the isolated RNA was analyzed by Agilent Bioanalyzer 2100. Only the RNA samples with an RNA Integrity Number (RIN) value of 6.0 or higher were included for RNA sequencing. RNA-seq libraries were constructed from RNA samples meeting QC criteria based on polyA+ selection of mRNA using a standard strand-specific protocol with VAHTS® Stranded mRNA-seq Library Prep Kit for Illumina (Vazyme, China, Cat. #NR612). The quality of the sequencing libraries was analyzed by Agilent Bioanalyzer 4200 and the peak length ranged from 250~500 bp. Clusters were generated by cBot with the library diluted to 10 pM and then sequenced on Illumina HiSeq 2500 instruments with 2 × 150 bp paired-end sequencing. All libraries were sequenced to an average depth of 52 ± 8.8 (mean ± s.d.) million reads (corresponding to 26 ± 4.4 million paired-end reads).
GEO Accession
GSM5681129