GSM5183508: A375 Sirt5 Knockdown (shRNA 546) Rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted in TRIzol (Invitrogen) 96 hrs. post-transduction from three wells and treated with RNAse-free DNAse I (Roche) for 1 hour at 37ºC, according to manufacturer's instructions. RNA libraries were prepared using standard Illumina protocols by the University of Michigan Ann Arbor Advanced Genomics Core
GEO Accession
GSM5183508