WGS of S. haematobium: Single adult male Senegal
High molecular weight genomic DNA was isolated from single adult males of S. haematobium representing four geographic locations (Zambia, Senegal, Mauritius and Mali) using a commercial kit (Chemagic STAR DNA Tissue Kit, Perkin Elmer). The DNA yield was estimated spectrophotometrically (Qubit fluorometer dsDNA HS Kit, Invitrogen), and DNA integrity was assessed by agarose-gel electrophoresis and then using a Bioanalyzer (2100, Agilent). High-quality genomic DNA was used to construct short-insert libraries (500 bp) using a TruSeq DNA library construction kit (Illumina) and paired-end sequenced as 100 nucleotide (nt) reads using the HiSeq-2500 platform (Illumina).