GSM5035383: siScrambled rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from cell using TaKaRa MiniBEST Universal RNA Extraction Kit (Takara, 9767).RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis Libraries were prepared by using KAPA Stranded RNA-Seq Library Prep Kit(Illumina). Briefly,Total RNA of each sample was used to prepare the RNA sequencing library 1)RNA fragment；2)Random primed first strand cDNA synthesis；3)dUTP based second strand cDNA synthesis;4)Adaptor ligation and PCR amplification.
GEO Accession
GSM5035383