GSM3899327: Breast cancer cells, AE/AE, 828 +TGFb_2; Mus musculus; ATAC-seq
75x10^3 cells were subjected to lysis and transposition reaction according to the protocol described previously (Buenrostro et al., 2015). Barcoded libraries were prepared according to the protocol described previously (Buenrostro et al., 2015). 75x10^3 cells were used in each transposition reaction using the Nextera Tn5 Transposase kit (Illumina, FC-121-1030) followed by purification using Qiagen MinElute PCR purification kit (Qiagen, 28004). Transposed DNA fragments were PCR amplified over 13 cycles using barcoded PCR primers and NEB High Fidelity PCR Master Mix (NEB, M0541). Libraries were purified using QIAquick PCR purification kit (Qiagen, 28104) and sequenced with Illumina Illumina NextSeq 500.