GSM5577598: BA_2_2w: 6-BA_2.0 mg/L_2 w; Dendrobium catenatum; RNA-Seq
Total RNA was isolated from phytohormone treated D. catenatum plantlets using the Plant RNA Isolation Mini Kit (BioTeke, Beijing, China) according to the manufacturer's recommendation. DNA contamination was removed with recombinant RNase-free DNase I (TaKaRa, Tokyo, Japan) and then mRNA was enriched with Oligo(dT) magnetic beads (Thermo Fisher Scientific, MA, USA). The purified mRNA was cut into small pieces using fragmentation buffer and reverse-transcribed to produce the cDNA library using mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA) as instructed by the manufacturer.
GEO Accession
GSM5577598