RNA-Seq of homo sapiens: peripheral blood
Total RNA was extracted from the CD4+T cells using the TRIzol reagent (takara) according to the manufacturers protocol. The samples were preserved at -80 C for further analysis. Firstly, the rRNA in the total RNA were removed by the the Ribo-Zero TM kit. Then the RNA were fragmented, followed by cDNA synthesis, end repair and A-Tailing. Then the ligation product was amplified by PCR.