GSM5577319: Cd34+ isolated cells [sample_27]; Homo sapiens; RNA-Seq
RNeasy kit (QIAGEN) Protocol: SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian User Manual (063017). RNA Fragmentation time is 4 minutes and PCR2-Final RNA-Seq Library Amplification is 15-16 PCR cycles depends on RNA input.  Reagent/kit: SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian Components (634418, Takara Bio USA, Inc.) Index: SMARTer® RNA Unique Dual Index Kit – 96U Set A (634452, Takara Bio USA, Inc.) Sequencing: NovaSeq 6000 System (20012850, Illumina)
GEO Accession
GSM5577319