title: GSM5577956: RNA-Seq Human colon cancer cells - clone D3 CTCF_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were fixed with 1% freshly prepared formaldehyde solution for 10min and then lysed with buffer containing: 50 mM Tris-HCl, pH 8; 10 mM EDTA; 1% SDS; x1 Proteinase inhibitor (Roche). Chromatin was sonicated and DNA–protein complexes were immune-purified with antibodies against CTCF (CS 2899S) RNA-seq library construction was performed with standard Illumina TruSeq Stranded mRNA kit with  Poly-A selection and samples were sequenced on NovaSeq6000 (NovaSeq Control Software 1.7.0/RTA  v3.4.4) with a 51nt(Read1)-10nt(Index1)-10nt(Index2)-51nt(Read2) setup using 'NovaSeqStandard'  workflow in 'SP' mode flowcell.