title: GSM6205736: HIBD input2; Rattus norvegicus; ChIP-Seq
library_construction_protocol: Briefy, after deeply anesthetized with chloral hydrate(200 mg/kg), the rats pups in Sham, HIBD and HIBD+SB groups were perfused with physiological saline from the right auricle until the liver turns white. The brain of rats was removed on ice, put into an ice-cold PBS and rapidly separated the infarct side cerebral cortex Illumina sequencing libraries were generated with NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) by following the manufacturer's manual. The library quality was determined by using Agilent 2100 Bioanalyzer (Agilent), and then, subjected to high-throughput 150 base paired-end sequencing on Illumina Novaseq sequencer according to the manufacturer's recommended protocol.