title: GSM4724370: Patient23_adjacent non-tumor [R23A] miRNA-Seq; Homo sapiens; miRNA-Seq
library_construction_protocol: The frozen tissues were added with RLT buffer (Qiagen, Germany) with 1% β-mercaptoethanol and then homogenized by gentleMACS dissociator (Miltenyi Biotec). RNeasy mini kit (Qiagen, Germany) was used to extract the total RNA following the manufacturer's protocol. Total RNA of each sample was used to prepare the miRNA sequencing library, which included the following steps: 1) 3'-adapter ligation; 2) 5'-adapter ligation; 3) cDNA synthesis; 4) PCR amplification; 5) size selection of ~130-150 bp PCR amplified fragments (corresponding to ~15-35 nt small RNAs).