title: GSM5889215: ATAC-seq - WT 3W-3 (pair 2); Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq: 5,000 FACS-sorted Tcf21 LPs were lysed to isolate the nuclei. Isolated nuclei were then incubated with the Tn5 transposase (TDE1, Illumina) and tagmentation buffer at 37°C for 30 minutes with shaking on a thermomixer at 500 g. Tagmentated DNA was purified using MinElute Reaction Cleanup Kit (Qiagen). ATAC-seq: PCR was performed to amplify the ATACseq libraries using Illumina TrueSeq primers and multiplex by indexes primers. After the PCR reaction, libraries were purified with the 1.1X AMPure beads (Beckman).