title: GSM5577903: multi_PYMT_cd11b_lo_myeloid; Mus musculus; RNA-Seq
library_construction_protocol: Tumors from different stages were digested with collagenase IV and Dnase I for 30min, cells were filtered and pooled. Cells were stained 20min at 4°C with the antibody mix and myeloid cells were sorted in total or enriched for CD11b+ or CD11b- myeloid cells, according to CD45+CD90.2-B220-NK1.1-Ly6G-CD11b+ or CD11c. After sorting, all cell preparations were pelleted and resuspended at 1x103cells/ml in 0.04%BSA/PBS and loaded onto the Chromium Controller (10X Genomics). Samples were processed for single-cell encapsulation and cDNA library generation using the Chromium Single Cell 3'v2 Reagent Kits (10X Genomics) according to manufacturer's protocol. The library was subsequently sequenced on an Illumina HiSeq4000 (Illumina).