title: GSM4773822: Sample1_5-22_S13_001    WT; Mus musculus; RNA-Seq
library_construction_protocol: Ribosome-bound RNAs were isolated as described previously (​47​). Briefly, somatosensory cortical sections (1 mm) from Fmr1+/yAldh1l1-TRAP and Fmr1−/yAldh1l1-TRAP mice were prepared on a mouse brain matrix. The anti-eGFP antibody (mouse clone HtzGFP-19C8, Memorial Sloan Kettering Cancer Center) was coupled to magnetic beads (Dynabeads, Invitrogen) as described in the kit instructions. Tissue homogenates were prepared and mixed with eGFP antibody-coupled beads for immunoprecipitation. Following immunoprecipitation, RNA was isolated using the TRIzol reagent and precipitated with isopropanol. Libraries were prepared according to Illumina's instructions with TrUSeq Stranded Total RNA with RiboZero Gold kit Pair-end (100bp) RNA-seq