title: GSM5188345: B1_Cre3; Mus musculus; RNA-Seq
library_construction_protocol: Harvested spleens were mechanically dissociated to form a single cell suspension. Erythrocyte lysis was carried out using ACK buffer and B cells were immunomagnetically selected from the resulting cell suspension using the MACS B cell Isolation Kit. Cells were washed in cold PBS and total RNA was extracted using Qiagen RNAeasy kit. Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina).