title: GSM4875931: AML; Homo sapiens; OTHER
library_construction_protocol: iCLIPs were performed as described in Herdy et al., Nucleic acids Res., 2018. For cRIP, cells were UV crosslinked and cytoplasmic lysates collected by hypotonic lysis. RNA was fragmented with RNaseA and subjected to affinity enrichment using BG4 or A9 antibody. RNA fragments were purified following de-crosslinking and proteinase K digestion using phenol/chloroform. Polysome profiling was performed as described in Herdy et al., Nat. Immunology, 2012. RNA was isolated using Trizol reagent (LifeTechnologies). iCLIP libraries were prepared as described in Huppertz et al., Methods, 2014. cRIP libraries were prepared using NEBNext® rRNA depletion kit and NEBNext® Ultra™ II Directional RNA Library Prep Kit. Polysome profiling libraries were prepared using TruSeq RNA library prep kit (Illumina).