title: RNA Seq of Staphylococcus aureus co-cultured with ethanol for 3H
design: RNA libraries were created from combined triplicate RNA samples of S aureus controls and S. aureus with mycolactone supplementation (300 ng/mL concentration) at 3H, 6H, and 24H. .  Libraries were created using the NEBNext Ultra RNA Library Prep Kit and NEBNext Multiplex Oligos (Dual Index Primers) for Illumina and associated protocols. High-throughput RNA sequencing was performed by St. Jude Childrens Research Hospital on an Illumina HiSeq2000 with 2 X 150bp PE (paired end) read lengths.