title: leaf
design: The rhizome and leaf tissue was ground in liquid nitrogen, and its RNA was extracted following the CTAB protocol. After extraction, each sample was treated with 1 l DNase at a concentration of 1 unit/g of total RNA to remove DNA contamination. RNA quality and integrity were assessed using the Nanodrop based on absorbance ratios at 260/280 num and Agilent 2100 Bioanalyzer. All samples selected for sequencing had an RNA integrity number over 8.