title: RNA-Seq of mus musculus: Female C57BL6 lung neutrophils
design: Neutrophils (CD45+ CD11b+ Ly6Clo Ly6G+) were FACS-purified from lungs of LF or HF DIO mice. All steps for RNA-sequencing and quality control was performed at GENEWIZ, NJ. RNA was isolated from using TRIzol LS reagent  (Invitrogen), and RNA integrity was assessed by an Agilent Bioanalyzer 2100. RNA-sequencing libraries were prepared using the SMART-Seq library preparation kit, and 2x100 base pair sequencing was performed on an Illumina HISeq 2000.