title: GSM4774848: AS aged spleen scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: CD45hi single cells were sorted by flow cytometry for single-cell RNA sequencing. 10x Genomics Chromium single-cell 3' v2 kit and protocol were used to prepare the libraries. HalioDx (Marseille, France) performed the RNA sequencing on a NextSeq500 machine with a sequencing depth of at least 50,000 reads per cell.