title: GSM5225586: Close stroma heifer 4; Bos taurus; RNA-Seq
library_construction_protocol: Mammary tissues were removed into RNA-save solution and kept at -380C. RNA was extracted with Trisol reagent. Total RNA was fragmented, followed by reverse transcription and second-strand cDNA synthesis. The double-stranded cDNA was subjected to end repair, a base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing. RNA-Seq; poly A selection