title: GSM5752987: hepatic stellate cells isolated at 6 degrees from the non-tumor region of high fat diet-fed mouse livers  [HSC_NT_6]; Mus musculus; RNA-Seq
library_construction_protocol: Hepatic stellate cells were isolated from mouse livers by perfusion method, and the cells were stained with antibodies against CD31, CD45.2, and CD49a. The cells in the CD31-/CD45.2-/CD49a+ fraction were sorted, and total RNA was extracted using Trizol. RNA libraries were prepared for sequencing using standard Illumina protocols.