title: GSM4997065: monos_1; Mus musculus; Bisulfite-Seq
library_construction_protocol: All populations were isolated from 8- to 12-week-old C57BL/6J mice by fluorescence activated cell sorting. The following hematopoietic cell populations were isolated from bone marrow cells: MDPs, CDPs, cMoPs and monocytes. Monocytes were sorted from total bone morrow.  MDPs, CDPs and cMoPs were sorted from lineage negative cells. Lineage depletion of mature hematopoietic cell lineages was performed using Sheep anti-Rat IgG magnetic beads and rat IgG isotype lineage antibodies. The following hematopoietic cell populations were isolated from splenic cell suspensions: pDC, cDC CD8a+, cDC CD11b+. DNA was isolated from snap-frozen cell pellets (10.000 - 25.000 sorted cells) using the QIAamp DNA Micro Kit (Qiagen) according to the manufacturer's instructions. Tagmentation-based whole-genome bisulfite sequencing (TWGBS) libraries were prepared as described previously (Wang et al., Nat. Prot., 2013). We used 10-30 ng genomic DNA as input.