title: GSM4996914: J_S7; Mus musculus; OTHER
library_construction_protocol: Total RNA was extracted by TRIzol according to the manufacturer's instructions. cDNA was synthesized by SMARTScribe Reverse Transcriptase with primers targeting mouse TCRa or TCRb chains and template switch adapter TCRa and TCRb chains were further amplified by a two-step protocol. Sequencing adaptors were then added by Kapa Hyper Prep Kit (Kapa Biosystems). Samples were pooled at the same molar ratios and sequenced by Nova Seq with at least 20x coverage relative to the numbers of Treg cell used.