title: HiSeq X Ten paired end sequencing; Primary monocyte to macrophage differentiation involves significant changes in chromosome conformation different from cultured THP-1 cells
design: Primary monocyte to macrophage differentiation involves significant changes in chromosome conformation different from cultured THP-1 cells
library_construction_protocol: Monocytes were isolated from peripheral blood of healthy volunteers and purified with  EasySepTM Human CD14 Positive Selection Kit II(STEM CELL,Catalog #17858). Macrophages were induced from monocyes with GM-CSF for 7 days. The protocol was followed by Rao.(A 3D Map of the Human Genome at Kilobase Resolution Reveals Principles of Chromatin Looping The protocol was followed by Rao.(A 3D Map of the Human Genome at Kilobase Resolution Reveals Principles of Chromatin Looping