title: Illumina MiSeq paired end sequencing; RNA extracted from a pool of matured Oikopleura dioica adults and sequenced on Illumina MiSeq platform.
library_construction_protocol: Total RNA was extracted from a pool of matured Oikopleura dioica adults and isolated with RNeasy Micro KitmRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760). Adapters for the creation of DNA libraries for the Illumina platform were added per manufacturerâ€™s guidance (NEB, E7805) as were unique indexed oligonucleotides (NEB, E7600) to each of the 3 staged samples. The cDNA library was sequenced paired-end with a 300-cycle MiSeq Reagent Kit v2 (Illumina, MS-102-2002) loaded at approximately 12 pM.