title: GSM4773497: A. aculeatus BCC56535 condition B [BCC56535_lot2_B]; Aspergillus aculeatus; RNA-Seq
library_construction_protocol: Fungal cell were cultured for 3 days at 30 degree, frozen with liquid nitrogen, and RNA was harvested using Trizol reagent, following RNAeasy kit (Qiagen), contaminated DNA was eliminate by RNAase free DNase kit (Qiagen). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols