title: Illumina HiSeq 4000 sequencing; RNA-seq of HUVECs in two models of CFTR blockade
design: RNA-seq of HUVECs in two models of CFTR blockade
library_construction_protocol: Control vs CFTR knockdown, and vehicle vs CFTRinh-172 (10ÂµM) treated endothelial cells were collected. RNA was extracted using the TRIzol kit following the manufacturer's instructions. Quality and quantity were measured on a Nanodrop. Sequence libraries were prepared with the Lexogen QuantSeq 3' mRNA-Seq Library prep kit according to the manufacturer's protocol. Samples were indexed to allow for multiplexing. Library quality and size range was assessed using a Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies) according to the manufacturer recommendations.