title: ITS metabarcoding of soil fungi: Antarctic Dry Valleys
design: The ITS1 region was amplified using ITS1F (Gardes & Bruns, 1993; Molecular ecology, 2(2), 113-118) and ITS2 (White et al., 1990; PCR protocols: a guide to methods and applications, 18(1), 315-322) primers, following the protocol described in Smith & Peay (2014; PloS one, 9(2), e90234). An equimolar pool of uniquely barcoded amplicons was paired-end sequenced (2  300 bp) on an Illumina MiSeq platform.