title: sRNA-Seq of Arabidopsis thaliana
design: For sRNA sequencing, 22-d-old seedlings of A. thaliana in MS medium were collected with two biological replicates. RNA samples were extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and treated with DNase I (Takara, Dalian, China) to remove DNA contamination.