title: Illumina HiSeq 1500 sequencing; Dark-induced senescence time course of ANAC017 mutant lines in Arabidopsis
design: Dark-induced senescence time course of ANAC017 mutant lines in Arabidopsis
library_construction_protocol: Individually darkened leaves 6 and 7 were collected and snap frozen. Plants were grown for 5 weeks in soil in standard long day growth conditions. Plants were randomized throughout the tray, shelf position and room position to compensate for environmental factors. Leaf 5 was collected for starch and ANAC017 and ANAC016 gene expression analysis. Leaf 6 and 7 was used for chlorophyll and ion leakage analysis. Plants were randomized throughout the tray, shelf position and room position to compensate for environmental factors. Leaf 6 and 7 were covered with aluminium foil for dark induced senescence and harvested at indicated time points. Total RNA was extracted using the Sigma Spectrum plant total RNA kit Libraries were constructed using the Illumina RiboZero Plant kit