title: GSM5348179: Mock_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Four thousand cells were lysed in 1X Lysis buffer (BioSpyder Technologies, Carlsbad, CA). Cell lysates were assayed using TempO-Seq whole human transcriptome targeted RNA sequencing plates , reagents, protocols, and software (BioSpyder Technologies). Using a thermocycler, lysed samples were added to the TempO-Seq 96-well plates, coded adjacent primer pairs for each specific human transcript were annealed to sample RNA, primer pairs for each transcript ligated then amplified per manufacturer's instructions .