title: Illumina HiSeq 2000 paired end sequencing; RNA-seq of male and female mouse brown adipose tissue (BAT) transcriptome
design: RNA-seq of male and female mouse brown adipose tissue (BAT) transcriptome
library_construction_protocol: C57BL/6J mice were purchased at 8 weeks old, acclimatized for one week, and sham-operated at 9 weeks old. After 45 days of recovery at basal laboratory conditions (chow at libitum, 22C), mice were fasted for 4 hours in the morning. Subsequently, mice were euthanized by cardiac puncture under isoflurane anesthesia, and the interscapular BAT depots were obtained. Total RNA was isolated using the TriPure isolation reagent (Roche Diagnostics, Mannheim, Germany) and contaminating genomic DNA was removed by the RQ1 RNase-free DNase (Promega Benelux, Leiden, Netherlands), according to the manufacturers' instructions. RNA quality was assessed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Amstelveen, Netherlands). A TruSeq RNA Library Prep Kit v2 (Illumina, Eindhoven, Netherlands) was used for library preparation according to the manufacturer's instructions.