title: GSM5512793: C (0.4×10^9/mL PA-MSHA treatment); Homo sapiens; miRNA-Seq
library_construction_protocol: RNA was harvested from cells using Trizol reagent. Illumina TruSeq RNA Sample was used with 1 ug of total RNA for the construction of sequencing libraries. miRNAs libraries start from the RNA that has passed the quality inspection, the 3' -end adaptor is linked, the reverse primer is hybridized, the 5' -end adaptor is linked, the reverse transcription is made into DNA, and then PCR amplification is performed.