title: GSM5191842: LHa4-5: GMP_Inflammation2; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow was flushed from femurs. Lineage depletion was performed with PE-coupled antibodies (anti-mouse CD4, CD8, CD19, B220, NK1.1, Ter119, CD11b, CD11c, Ly6G, Il7Ralpha), anti-PE microbeads (130-097-054, Miltenyi Biotec) and magnetic separation,  (130-042-901, LD columns, Miltenyi Biotec, Bergisch Gladbach, Germany). RNA was extracted from flow sorted cells using the NucleoSpin RNA XS Kit (740902.50, Takara Bio, Kusatsu, Japan). Then Lin(neg)Sca1(pos)ckit(pos)CD34(pos)CD16/32(pos) was sorted for downstream process NGS library construction was performed using Clontech SMARTer Ultra Low Input RNA kit for Illumina (634936, TaKaRa Bio USA, Mountain View/CA, USA).