title: RNA-Seq of root: soybean
design: 7 days after the soybeans were sowed, they were inoculated with rhizobia (BXYD3) and cultured in soybean nutrient solutions with different nitrogen concentrations (HN 5300 M; LN 100 M) for 5 days. Collect soybean roots for RNAseq sequencing and data analysis.