title: GSM5893519: Arabidopsis thaliana jar1-11 D4; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted from the whole rosette leaves of 32 day-old plants using the Quick-RNA Miniprep Kit (Zymo-Research, USA). Approximately 200 ng of RNA was used for library construction. Sequencing libraries were prepared using the Lexogen's QuantSeq 3'-mRNA-Seq Kit and sequenced on an Illumina HiSeq 2500 V4 platform with a read length of 1x50 bases. For each of the samples, three biological replicates were sequenced with an average sequencing depth of 10 million reads.