title: Illumina NovaSeq 6000 paired end sequencing; RNA-Seq of mouse microglia under LPS stimulation and miR-106 overexpression
design: RNA-Seq of mouse microglia under LPS stimulation and miR-106 overexpression
library_construction_protocol: PBS- and LPS-treated microglia were transfected with either miR-106b or inhibitor controls for 2 days. RNAs were isolated from cells using Qiagen RNeasy mini kit according to the manufacturer's instructions.  Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® following manufacturer's instructions and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS following manufacturer's instructions.