title: Illumina HiSeq 2000 paired end sequencing
library_construction_protocol: The RNA-Seq libraries were prepared with KAPA Stranded mRNA-Seq Illumina Platforms Kit (KAPA Biosystems) following the manufacturers recommendations. Briefly, 500ng of total RNA was used as the input material, the poly-A fraction was enriched with Oligo-dT magnetic beads and the mRNA was fragmented. The strand specificity was achieved during the second strand synthesis performed in the presence of dUTP instead of dTTP. The blunt-ended double stranded cDNA was 3' adenylated and Illumina indexed adapters were ligated. The ligations product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer with the DNA 7500 assay.