title: GSM5171977: High_glucose; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction method: Trizol  The first step is to build a circular RNA library and remove ribosomes, using RiboMinus Human/Mouse Transcriptome Isolation Kit (invitrogen, thermofisher); the second step is to remove linear RNA, using RNase R enzyme (epicentre, illumina), and the third step is to construct Library, using KAPA Stranded mRNA-Seq Kit (roche) A RNA library was constructed to remove ribosomes, using RiboMinus Human/Mouse Transcriptome Isolation Kit (invitrogen, thermofisher)