title: GSM5543299: BU #2; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were resuspended at ~200 viable cells/ml in PBS containing 2% FBS prior to library preparation using a 10X Chromium controller CTRL #1 and BU #1 (pooled from 3 mice for each group): Library construction was performed using a 10X Chromium controller with the Chromium Single Cell 3' Reagent Kit V2. Both libraries were sequenced in one high-output lane of an Illumina NextSeq in single-read 150b format (115b effective read length) CTRL #2 and BU #2 (pooled from 3 mice for each group): Library construction was performed using a 10X Chromium controller with the Chromium Single Cell 3' Reagent Kit V3.1. Both libraries were sequenced in one high-output lane of an Illumina NextSeq. single-cell RNA-seq (10x genomics)