title: GSM4774836: Sample 18: Tumor-bearing PPM1D transgenic mouse; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction and sequencing was performed by Otogenetics, Atlanta, USA. Total RNA was extracted using the Rneasy Mini Kit (Qiagen). RNA quality was assessed using the Agilent Bioanalyzer platform. Sequencing libraries were prepared using the TruSeq Stranded cDNA kit. Paired-end reads of length 100-125 bp were generated using the Illumina HiSeq 2500 platform. Sequencing depth was approximately 40 million reads for each sample.