title: GSM6050580: HL-60(Human Transcriptome Seq); Homo sapiens; RNA-Seq
library_construction_protocol: Cells in logarithmic growth phase were collected, and we extracted total RNA using TRIzol reagent,The mRNA was isolated from total RNA with oligo(dT) magnetic beads (ThermoFisher), and then subjected to immunoprecipitation with the GenSeqTM m7G-IP Kit (GenSeq Inc., China) by following the manufacturer's instructions. Briefly, total RNA was used for removing the rRNAs with NEBNext® rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA) following the manufacturer's instructions. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, USA). Library sequencing was performed on an illumina NovaSeq 6000 instrument with 150bp paired end reads.