title: GSM4816929: WT_control; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA fraction was isolated from cultured NPC samples with Trizol reagent (Thermo Fisher) followed by total RNA extraction using the RNA Clean and Concentrator-25 (Zymo Research). mRNA was isolated from total RNA with Dynabeads mRNA purification kit (Thermo Fisher). For samples WT1 through WT4 and KO1 through KO4, RNA sequencing libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina, and each band of each sample used a unique index PCR primer (NEBNext Multiplex Oligos for Illumina). Libraries were multiplexed 1:1 for sequencing in the NextSeq 500 (Illumina) yielding approximately 100 million 2×75-bp paired-end reads per sample. For sample WT_control, libraries was prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina and sequenced in Illumina HiSeq 2500 yielding approximately 30 million 50 bp single-end reads.