title: GSM6094642: Kasumi1 shCTRL rep3; Homo sapiens; ATAC-seq
library_construction_protocol: A total of 60000 cells were lysed with digitonin and tagmented with an engineered Tn5 transposase at 37°C for 30 minutes, a protocol optimized for blood cells. Tagmented DNA was purified using the MinElute Reaction Cleanup kit and then amplified by PCR. Before the sequencing, fragments with a 1-5 kb size range were removed by magnetic separation with AMPure XP beads. Library were sequenced using Illumina High throughput Sequencing technology. Samples were loaded on NovaSeq 6000 and sequenced in paried-end mode, generating 100 nt length reads in order to obtain approximately 40M reads.