title: GSM6159405: M11 scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Spleens from autoimmune and immunized chimeras were processed following viability recommendations for droplet-based Chromium single cell RNA-seq gene expression (10X). The full organs were dissociated in MACS buffer (PBS 1X, 0.5% BSA, 2nM EDTA) using syringe plungers and 70um cell strainers, spun down at 1000rpm for 5 min, resuspended in RBC lysis buffer and incubated on ice for 5 min. Samples were washed with MACS buffer and spun down 1000 rpm for min. Cells were enriched by negative selection with a Pan II B cell enrichment kit (Miltenyi) according to the provider specifications. During enrichment incubation cells were also stained for flow sorting Barcoded RNA libraries for  gene expression and BCR vdj repertoire were processed according to the manufacturer's instructions (10x Genomics) for Chromium Single Cell Immune Profiling.