title: GSM5348210: ChIP-ES-betacatenin-highdensity_r2; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIP-seq, chromatin was sheared from formaldehyde-fixed mouse embryonic stem cells. Protein-DNA complexes of interest were pulled down with magnetic streptaviding beads that were extensively washed in buffer before elution. For RNA-seq, total RNA was isolated using the RNeasy Mini Plus Kit from Qiagen and quantified on a Nanodrop. For ChIP-seq, libraries were prepared according to the manufacturer's instructions in the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB) and sequenced on a NovaSeq 6000. For RNA-seq, paired-end libraries were prepared according to the manufacturer's instructions in the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB) and sequenced on a NovaSeq 6000. Unpaired libraries were prepared using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB) and sequenced on a NextSeq500.