title: Illumina HiSeq 4000 paired end sequencing; Transcriptome of primary acute myeloid leukemia (AML) cells treated with AICAr
design: Transcriptome of primary acute myeloid leukemia (AML) cells treated with AICAr
library_construction_protocol: RNA-seq transcriptome analysis of primary AML cells isolated from the bone marrow of a patient with de novo FAB-M2 AML. Mononuclear cells were isolated from the bone marrow sample by density gradient separation and grown in RPMI supplemented with 10% FBS, 2 mM L-glutamine, 50 U/ml penicillin, 50 μg/ml streptomycin and 50 ng/ml IL-3, IL-6, SCF and FLT3L. Cells were treated with AICAr (0.4 mM) for 24 h. Experiment was performed in biological triplicates.                    Total RNA was isolated and purified for polyA mRNA Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina