title: RNAseq of AGS cell line in stiff matrix
design: For library construction, RNA is extracted from a sample. After performing quality control(QC), qualified samples proceed to library construction. The sequencing library is prepared by random fragmentation of the cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified. For cluster generation, the library is loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. Each fragment is then amplified into distinct, clonal clusters through bridge amplification. When cluster generation is complete, the templates are ready for sequencing. Sequencing data is converted into raw data for the analysis.