title: GSM4773522: PCa2; Homo sapiens; RNA-Seq
library_construction_protocol: Cancerous prostate tissues were cut into small pieces and digested in a lyase cocktail for the isolation of single cells. Cells were combined with barcoded Gel Beads, cells enzyme and partitioning oil to generate GEMs. After GEMs generation, copartitioned cells were lysed and the polyA mRNA were released and incubated with a Master Mix containing reverse transcription reagents to synthesize the full-lengh cDNA. Libraries were prepared according to Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (PN-1000121). Briefly, silane magnetic beads were used to purify the first-strand cDNA from the  post GEM-RT reaction mixture.The full-length cDNA were amplifed via PCR to generate sufficient mass for library construction. After end repair, A-tailing,  adaptor ligation and PCR amplification, the  final libraries were ready for sequencing.