title: GSM4996424: D67dep_lane1_20200703000; Homo sapiens; RNA-Seq
library_construction_protocol: isolated from the coculture by negative selection and flow cytometric sorting. RNA was extracted from isolated NK cells using RNeasy Mini Kit (QIAGEN). Transcription profiling using RNA-Seq was performed using manufacturer recommended protocols. Briefly, 500 ng of DNase I-treated total RNA was used to enrich for poly-A containing transcripts using oligo(dT) primers bound to beads. The enriched RNA pool was then fragmented, converted to cDNA and ligated to sequencing adaptors containing indexes using the Illumina TruSeq stranded mRNA sample preparation kit (Illumina). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer and combined equally into pools for sequencing.