title: GSM5889062: SW002 Plate 2; Danio rerio; RNA-Seq
library_construction_protocol: Tails were amputated at the tip of yolk extension in ice cold PBS, dissociated using liberase, buffered to 0.1% FBS, and filtered for flow cytometry. Single live EGFP+mCherry+ or EGFP+mCherry- cells were sorted into individual wells of 384 well plates, with both sample types represented on each plate to account for potential batch effects. Each well contained 50 nl droplets of barcoded RT primers in 10uL of mineral oil. RNA libraries were prepared for sequencing using the SORT-seq protocol - see Muraro et al. 2016 (Cell Syst).