title: GSM3457537: Amb_R2_T1; Megathyrsus maximus; RNA-Seq
library_construction_protocol: 100 ul RNA extracted and shipped to UIUC in 250ul EtOH(100%). EtOH removed. 10ul NaAC (pH 5.5) added with 1 ul glycerol. 30 min spin, remove supernatant. 250 ul EtOH (80%). 15 min spin, EtOH removed. Air dry 10 min. 50ul RNA treated H2O. 5ul aliquot The libraries were prepared with Illumina's TruSeq Standard RNA sample prep kit. (for the MiSeq library, 500 ng of total RNA from each sample were pooled and prepared with this kit with one modification: RNA was not fragmented). MiSeq Sequencing of pooled RNA: library was quantitated by qPCR and sequenced on one MiSeq flowcell for 301 cycles from each end of the fragments using a MiSeq 600-cycle sequencing kit version 3. HiSeq Sequencing of Pools: libraries were quantitated, pooled in equimolar concentration, quantitated by qPCR and sequenced on two lanes for 161 cycles from each end of the fragments on a HiSeq2500 using a TruSeq SBS Rapid sequencing kit version 1.