title: GSM5971694: 3MC-C1 shRNA Erk5 rep1 [C11SH]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from cell and mice tumor samples (after tissue homogenization with a Polytron), were performed as previously described cDNA libraries were generated from 600 ng of RNA from each sample using the Illumina TruSeq Stranded mRNA Sample Preparation kit (Illumina). Libraries were sequenced on an Ilumina HiSeq2000 apparatus according to the manufacturer's protocols