title: GSM5889087: VX_KJ_LATS-S9-2; Mus musculus; RNA-Seq
library_construction_protocol: EYFP+ live cells were isolated using FACS. Calcein blue AM (Invitrogen) was used as a live cell marker at a concentration of 1 µM. FACS-sorted cells were lysed using Trizol LS. RNA was extracted using MaXtract high density columns (Qiagen) and the RNeasy Mini Kit (Qiagen). Libraries were prepared using the Illumina® TruSeq® RNA Sample Preparation Kit v2 according to the manufacturer's protocol.