title: 16S-Amplicon
design: The 16S rRNA gene variable region V4 was sequenced using an Illumina MiSeq sequencer by the Next Generation Sequencing Service at Molecular Research LP (MR DNA, Shallowater, TX, USA). The PCR primers 515F (5-AATGATACGGCGACCACCGAGATCTACACTATGGTAATT GT GTGCCAGCMGCCGCGGTAA-3) and 806R (5-CAAGCAGAAGACGGCATACGAGATTCCCTTGTCTCC AGTCAGTCAG CCGGACTACHVGGGTWTCTAAT-3) were used and paired-ends reads of 312 bp were obtained.