title: GSM5542628: Treg Ctrl 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from Th17- and Treg-polarizing T cells with or without ITA on day2 using RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's instructions. For RNA-sequencing, total RNA was quantified and qualified by NanoDrop and Qubit RNA Assay (Thermo Fisher Scientific), and by RNA ScreenTape on TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). 500 ng total RNA with RIN 7 or higher was used for poly-A mRNA enrichment (NEBNext Poly(A) mRNA Magnetic Isolation Module) and the following library preparation by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to the manufacturer's protocol.