title: GSM5511465: db_DMSO_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from Trizol reagent with Chloroform and RNA quality was determined (RIN>7) via Agilent Bioanalyzer Libraries were prepared according to BGI in-house kit instructions accompanying the RNA Sample Kit. cDNA synthesis, end-repair, A-base addition, and ligation of the indexed adapters were performed. The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA) and Qubit fluorometry (Invitrogen, Carlsbad, CA).