title: GSM6206855: Haltere Input Rep2; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Wing and haltere imaginal discs were crosslinked using 1.5% para-formaldehyde solution. Sonication was perfomed using the Covaris S2 sonicator. Chromatin Immunoprecipitation was perfomed using polyclonal Ubx antibodies raised against the N-terminal region. Equal amounts of DNA (~2 ng) was used as an input fo each sample and libraries prepared using standard protocol using NEB Ultra II DNA library prep kits (NEB #E7645). Number of cycles for amplification of adapter ligated libraries were estimated by the qPCR before final amplification to avoid any bias arising due to PCR amplification and indexing (NEB #E7350).