title: GSM4875569: KMT2D_ChIP-seq_24h_rep1; Mus musculus; ChIP-Seq
library_construction_protocol: We made following modifications to the parental protocol. To shear chromatin, we treated chromatin with 2 ul MNase (NEB M0247) in 90 ul reaction for 30 min at 25°C, quenched the reactions by adding 5 ul of 100 mM EDTA, and then sonicated the samples with QSonica Q800R at 70% amplitude for total 30 min at 4°C. w Before IP, we spiked in cross-linked chromatin from 0.25 million Drosophila S2 cells in each ChIP reaction. We performed internal ChIP control by an in-house polyclonal rabbit antibody against H2Av (1.6 ug), the fly specific histone variant. The sequencing libraries were prepared using NEBNext Ultra™ II DNA Library Prep Kit for Illumina. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® Input DNA from all the  samples are mixed.