title: GSM5880454: HepG2-input-2; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were incubated with protein A, Dynabeads, and 5 μg antibody overnight at 4 °C. After immunoprecipitation, the magnetic beads were recovered with a magnet and washed. DNA was eluted, de-crosslinked at 65 °C for 4 h, and then purified with the QIAGEN Kit. Sequencing libraries were constructed with 5 ng of purified ChIP DNA and they were sequenced using Illumina HiSeq X Ten.