title: GSM5604912: X_108_blood; Homo sapiens; RNA-Seq
library_construction_protocol: Pleural fluid samples from each subject were collected in heparin-treated tubes within 24 hours after hospitalization, using standard thoracentesis techniques. Approximately 5 mL of venous blood were drawn simultaneously. The pleural effusion specimens were immediately immersed in ice and were then centrifuged at 1,200 rpm for 5 min. The cell-free supernatants of PE and sera were frozen at -80°C immediately after centrifugation. Mononuclear cells from pleural ﬂuid and blood were isolated by Ficoll-Hypaque gradient centrifugation within 1 hour and were resuspended in PBS. Single-cell libraries were constructed using the Single Cell 5′ Library & Gel Bead Kit, Single Cell V(D)J Enrichment Kit, Human T Cell, or Single Cell V(D)J Enrichment Kit, Human B Cell. According to the cDNA synthesis and Chromium Single Cell A Chip Kits, cell suspensions were loaded on a Chromium Controller to generate single-cell gel beads in the emulsion. The cDNA libraries were constructed using Single Cell 5′ Library Construction Kit and i7 Multiplex Kit.