title: GSM4996886: ATAC_mouse_NK_WT_ATAC_IL18_3; Mus musculus; ATAC-seq
library_construction_protocol: Fresh cells were prepared as previously described (Buenrostro et al., 2013). Briefly, sorted, stimulated cells were washed in cold PBS and lysed. The transposition reaction was incubated at 37°C for 30 minutes. The DNA was cleaned with the MinElute PCR Purification Kit (QIAGEN catalog # 28004). DNA was amplified for 5 cycles. After evaluation by real-time PCR, 8-11 additional PCR cycles were done. The final product was cleaned by aMPure XP beads (Beckman Coulter catalog # A63882) at a 1X ratio, and size selection was performed at a 0.5X ratio. Libraries were sequenced on a HiSeq 4000 in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit (Illumina).  An average of 36 million paired reads were generated per sample.