title: GSM5578167: WT 10μM GSK-3484862 d14 (MedChemExpress); Mus musculus; Bisulfite-Seq
library_construction_protocol: Cell pellets were snap frozen and kept at –80 °C until extraction. RNA and DNA was isolated simultaneously using the AllPrep DNA/RNA kit (Qiagen) Genomic DNA (500 ng) with spiked-in lambda DNA (1.25 ng) (NEB) was sheared using an M220 ultrasound sonicator (Covaris) to an average size of 350 bp. The size of the DNA fragments was confirmed by electrophoresis on an 1.5% agarose gel. 200 ng of sheared DNA was subjected to bisulfite conversion using EZ DNA Methylation-Gold Kit (Zymo Research) as per manufacturer's instructions. Libraries were prepared using 50-100 ng of bisulfite-converted DNA and the Accel-NGS Methyl-Seq DNA Library prep kit (Swift Biosciences) according to manufacturer's instructions, with 8-11 cycles of PCR-amplification. 