title: NSCLC patients PBMC
design: TCR seq: Genomic DNA was extracted from frozen PBMC specimens by using a High Pure PCR Template Preparation Kit (Roche). Multiplex PCR amplification of the CDR3 region of the TCR  chain was done in two rounds, PCR1 and PCR2. The first round, PCR1, was done with specic primers against each V and J gene. Primer sequences were filed as part of a Chinese patent (CN105087789A). The second round of PCR29 was done with universal primers.