title: GSM5393283: MS7_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with miRNeasy Mini Kit (Qiagen-217004) according to the manufacturer's instructions. The purity of the RNA was verified using a NanoDrop at 260 nm, and the quality of the RNA was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies) Libraries were prepared using KAPA Stranded mRNA-Seq Kit. mRNA was enriched by ribosomal RNA depletion. Library quality was checked using Agilent TapeStation analyzer and sequencing was performed with NovaSeq (2X100, 25 M reads per sample).