title: GSM5171791: B 20, rep2; Candida albicans; RNA-Seq
library_construction_protocol: RNA was extracted using the Ribopure Yeast Kit (Ambion).  The total RNA extraction was performed as per the mannual described in the Kit protocol. Briefly, Candida albicans grown in serum for different time points were washed with the PBS and then the total RNA was extracted using the Ribopure yeast kit. RNA purity was confirmed by the nandrop and RNA integrity were confirmed by analysis on an Agilent 2100 bioanalyzer, using the RNA 6000 nano kit (Agilent) to conform RINs above 8.0. The YPD grown samples was also extracted with the above kit and the RNA purity was checked by Nanodrop. RNA library was prepared as recommended by the IlluminaTruseq RNA sample preparation kit V2 for serum +butanol grown samples and sequencing was performed using Genome Analyzer IIx for 36 cycles. For the YPD grown sample RNA library was prepared using Ribo-zero gold kit and sequencing was performed on Illumina Hiseq for 150 cycles.