title: GSM5392441: lp_cell_hash; Mus musculus; RNA-Seq
library_construction_protocol: Colonic samples were resected from mice and dissected into small pieces, followed by incubation in RPMI with 3% fetal calf serum and 20 mM HEPES for 20 minutes at 37 degrees C with constant stirring. Tissues were then further digested with 250 mg/mL Liberase CI and 500 mg/mL DNAseq I with continuous stirring at 37 degrees C for 30 minutes. Single cell suspensions were stained with CD45/MHCI (Biolegend TotalSeq-C) for cell hashing for 10 minutes on ice. Cells from individual mice (3 per group) were pooled for single-cell RNAseq analysis from each tissue. Libraries were contructed accoding to the manufactuerer's protocol for the 5-prime v1 kit from 10X Genomics.