title: GSM5578229: Control_1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Sinoatrial node was dissected, flash frozen on LN2, and RNA extracted using RNeasy kit (QIAzol, Qiagen). Total RNA-Seq library construction was performed using the Swift Biosciences RNA Library Kit (Swift Biosciences, Ann Arbor, MI), following rRNA depletion using Lexogen RiboCop Depletion Kit Human/Mouse/Rat v2 (Lexogen Inc., Greenland, NH). Final library concentration was measured via the Qubit 1X dsDNA HS Assay kit (ThermoFisher Scientific) and library size was evaluated on the 4200 TapeStation using the Agilent D1000 ScreenTape System (Agilent Technologies).