title: GSM6095884: hAOEC cells - unirradiated - 24h control2 [0Gy24h_2]; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed lysed directly in Trizol. Phenol-chloroform extraction and total RNA isolation was performed as per manufacturer's guidelines using miRNeasy kit (Qiagen). lncRNA sequencing was performed by Novogene (Novogene, Sacramento, CA) using the TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion kit (Illumina, US) following manufacturer's instructions. Sequencing runs were performed on Novaseq 6000 S4 system (Illumina, US). Briefly, Poly-A tailed RNA was purified from total RNA using poly-T oligo attached magnetic beads. The purified RNA was fragmented and reverse-transcribed into first strand cDNA using random primers. Second strand cDNA synthesis was performed using dUTP to preserve strand specific information. 3'-ends were repaired with adenylation. Adapters were ligated for strand selection and sequencing priming. PCR amplification was followed by purification. The quality of the amplified library was assessed on Bioanalyzer, and libraries were quantified on Qubit Fluorometer (Themo Fisher Scientific, US). Sequencing reactions were performed to generated 150 bp paired-end reads.