title: NextSeq 500 paired end sequencing; Transcriptional profiling of the tuberculin skin test in individuals cured of active tuberculosis disease (RNA-Seq)
design: Transcriptional profiling of the tuberculin skin test in individuals cured of active tuberculosis disease (RNA-Seq)
library_construction_protocol: TSTs were performed by intradermal injection of 2U Tuberculin (Serum Statens Institute) into the forearm. 3-mm punch skin biopsies were taken at 48h and collected into RNAlater (Qiagen) for RNA extraction. Total RNA from skin biopsies was obtained with the Precellys Evolution homogeniser and Qiagen RNeasy mini kit. The KAPA mRNA HyperPrep Kit (Roche Diagnostics) was used to construct stranded mRNA-Seq libraries from up to 500 ng intact total RNA