title: Ion Torrent PGM sequencing of SAMD00245768
library_construction_protocol: Purified RNA was qualified and quantified using an Agilent Bioanalyzer (Agilent) and rRNA was eliminated using an NEBNext rRNA Depletion kit (New England Biolabs, MA, USA); a SMART-Seq Stranded Kit (#634442, Takara Bio) was then used to construct an RNA-seq library according to the manufacturers protocol. The RNA-seq library was further processed to be applied to an Ion PGM system (Life Technologies, CA, USA) using an Ion Plus Fragment Library Kit (Thermo Fisher Scientific) as instructed.