title: GSM4817604: MPs_ KO_ rep2 [MeDIP-seq]; Mus musculus; MeDIP-Seq
library_construction_protocol: Genomic DNA from 1x10e6 FACS sorted p7 MPs from one animal was isolated with the All Prep DNA / RNA / Protein Mini Kit (Qiagen) and used as one sample. Replicate samples from each genotype were used. The low input MeDIP protocol (PMID: 22402632) was used for library preparation. Libraries were amplified in a final PCR step using barcoded TruSeq primers (Index_prime; 12 cycles) and size selected on a 2 % agarose gel (230-320bp, peak at 282-294bp).