title: GSM4775056: ATAC_mTOR_KO_2; Mus musculus; ATAC-seq
library_construction_protocol: 50,000 sorted HSC cells from poly(I:C)-treated WT control, mTOR KO and mTOR KO;c-Myc Het mice were lysed in a cold lysis buffer. Transposase reaction were performed for 30 min at 37 °C immediately after the nuclei preparation. The samples were purified using a Qiagen MinElute kit. The ATAC-seq libraries were amplified using NEBnext PCR master mix followed by purified using Qiagen MinElute PCR Purification Kit (Qiagen, 28004), and then run on the Illumina sequencer HiSeq 2500.