title: GSM5511584: MCZ3; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction from flash frozen livers was performed using the miRNeasy mini kit (Qiagen) and quantified on the Infinite M200 Pro (Tecan). Quality of the RNA was evaluated on the 2100 bioanalyzer (Agilent). Novogene (Sacramento, CA) performed the RNA sequencing library preparation with the NEBNext Ultra RNA Library Prep Kit for Illumina by following the manufacturer's recommendations (NEB, Ipswich, MA). Agilent 2100 Bioanalyzer System (Agilent Technologies) was used to validate sequencing libraries that were subsequently quantified using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and by quantitative PCR (Applied Biosystems, Foster City, CA). NovaSeq 6000 with a 2x150 Paired End (PE) configuration was used for the libraries sequencing. Sequences were quality-checked using FastQC for completeness, depth and read quality.