title: GSM4724322: db BSO rep1; Mus musculus; RNA-Seq
library_construction_protocol: After culture, cells were washed with phosphate-buffered saline (PBS), and collected for RNA isolation. The cDNA synthesis and amplification were done as recommended by Clontech. cDNA libraries were prepared using Nextera XT DNA Sample preparation reagents. Fragmented and tagged libraries were quantified using an agilent bio-analyzer, pooled and sequenced on a HiSeq 2500 platform (Illumina) using the pair-end 75-bp sequencing strategy.