title: GSM6205306: BR30; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were lysed using TRIzol reagent and  processed for total RNA extaction with PureLINK RNA Mini Kit according to manufacturer's  instructions Library preparation was performed starting from 500 ng of total mRNA (Illumina, TruSeq Stranded mRNA, 20020594) according to the manufacturer's instructions. Indexed libraries were quality controlled on Agilent Bioanalyzer 2100 (High Sensitivity DNA kit), quantified (Qubit dsDNA HS Assay, Q32851), normalized and pooled to perform a multiplexed sequencing run.