title: GSM4875625: OF2; Mus musculus; RNA-Seq
library_construction_protocol: Hcrt neurons were labeled by reporter virus injection into yound and aged Hcrt:cre mice. Specifically, 0.3 μl AAV vectors carrying genes encoding Cre-dependent DsRed- hTRF2 (AAV-DJ-DIO-DsRed-hTRF2, 1.95 × 1012 genome copies per ml, customer virus packaged at Stanford Virus Core) were bilaterally injected to the LH (AP: −1.35 mm, ML: ±0.95 mm, DV: −5.15 mm) of young (3 months) and aged (18 months) Hcrt::Cre male and female mice with both genders to label telomere in the nuclei of Hcrt neurons. Lateral hypothalamus was dissected, nuclei liberated, and facs sorted for reporter signal. Sorted nuclei were then processed using 10X v3 3'end singel cell RNA seq pipeline 10x Genomics Chromium Single Cell 3' Reagents v3 snRNAseq