title: GSM4995975: B-ALL patient A52-2 replicate2; Homo sapiens; RNA-Seq
library_construction_protocol: 32 samples from  25 B-ALL patients' bone marrow samples were processed for RNA extractions and sequencing. For 7 patients two independent RNAseq experiments were performed (duplicates). For each sample, 1 µg RNA samples (RIN = 10) were used for libraries preparations with TruSeq Stranded Total RNA (RiboZero Human/Mouse/Rat) Library Prep (Illumina, Cat#RS-122-2201) according to manufacturer's instructions. Each library was quantified on Qubit with Qubit® dsDNA HS Assay Kit (Life Technologies) and the size distribution was examined on the Fragment Analyzer with High Sensitivity NGS Fragment Analysis kit (Agilent). Library prepared from B-ALL patients' samples were sequenced on Hiseq 4000 (PE150) platform in Novagene company.