title: GSM4817203: NexCre Control 2; Mus musculus; RNA-Seq
library_construction_protocol: P0 brains were dissected in ice-cold HBSS, and 200 µm-thick sections were cut using a vibratome (VT1000S). The lowermost part of the cortical plate (presumptive subplate) was visually identified and micro-dissected under stereomicroscopic guidance in ice-cold HBSS under RNase-free conditions. Microdissected subplate tissue was stored in Buffer RLT Plus (Qiagen) at -80°C and RNA was extracted using RNeasy PLUS Micro kit (Qiagen). mRNA was isolated from total RNA, cDNA was prepared, and libraries were prepared using the IlluminaTruSeq RNA Sample preparation V2. For RNA-seq, libraries were multiplexed and sequenced as 100 base-pairs (bp) pair-end reads using the Illumina HiSEQ2500 platform, at an expected depth of 60M single reads.