title: GSM5171711: drm2_gp2_IPARE_rep1; Arabidopsis thaliana; RIP-Seq
library_construction_protocol: RNA-immunoprecipitation was performed as described in Rowley et al., 2013 (PMID:23707621) using NF-pellet paint instead of Glycoblue. Experiments were performed with protein A agarose beads coated with salmon sperm DNA (for anti-NRPE1) Library was prepared by a modified version of NanoPARE protocol (PMID: 30355603). RNA was polyadenylated with Poly(A) polymerase. The polyadenylated RNA was EtOH-precepitated. The deadenylated RNA was DNase treated with TURBO DNase. The RNA was EtOH precepitated. The RT, template switching, pre-amplification and 2nd PCR were performed as NanoPARE. RIP-seq with modified NanoPARE : IPARE