title: GSM5171819: Acomys cahirinus UUO day 5 rep3; Acomys cahirinus; RNA-Seq
library_construction_protocol: Age-matched adult male C57BL/6J (B6) and A. cahirinus (2-4 months) were subjected to unilateral ureter obstruction (UUO) procedure at the left ureter. Kidneys from sham operated controls and UUO-treated animals after 2 and 5 days were collected after perfusion with ice-cold normal saline and flash frozen in liquid nitrogen. Total RNA was extracted from kidney using Trizol (Invitrogen) and Direct-zol RNA Kit (R2072, Zymo Research) according to manufacturers instructions. RNA libraries were prepared for sequencing using standard Illumina protocols