title: GSM5542960: Zygote; Mus musculus; RNA-Seq
library_construction_protocol: At late 1-cell stage, zona pellucida was removed by treating in acid Tyrode's solution. Then embryos were transfered to 0.25% Trypsin and PB2 was split from the embryo by continuous pipetting. Samples were collected into lysis buffer from Vazyme Single Cell Full Length mRNA-Amplication Kit (N712) for mRNA amplification. RNA libraries are prepared for sequencing using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) protocols.