title: Lepidochelys olivacea DNA genomic Nanopore basecallguppy
design: We extracted genomic DNA from 700 I of hatchlings of olive ridley's blood in 2 ml tubes Eppendorf with 7 I of EDTA (0.342 M, pH 7.2). Centrifugated and 800 I of Genomic lysis buffer (Quick-DNA Microprep Kit, Zymo Research, #D3020) was added to the cell pellet and left 10 min. Transferred to a column of RiboPure Blood kit (Invitrogen, #AM1928), centrifuged at 1000 G and washed three times with wash buffer (Zymo Research, #D4003-2-24), two washes with 300 I of RW1 buffer (Qiagen, #1053394) and two washes with 500 I with RW1 buffer. Finally, the column was eluted with 30 I of DNAse-free water. We obtained over 1 ug of high-quality large size DNA. Genomic DNA was sequenced using five Oxford Nanopore (MinION) sequencing devices. Base-calling was performed using the Guppy data processing toolkit (https://nanoporetech.com/nanopore-sequencing-data-analysis).