title: GSM4996762: Thymocytes, group2 WT; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspension were prepared from thymus and stainned with CD69 and TCRβ. Then, group 2 (TCRβintCD69hi) and group 3 (TCRβhiCD69hi) subsets were sorted by Flow cytometry. The total RNA were extracted by Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols