title: GSM4875616: Exp_d2_empty_control_2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted with TRIzol and chloroform, cleaned using the Qiagen Rneasy Mini kit, and Dnase treated (Qiagen 79254) for 30 min. 1 ug of RNA was used for library construction. For the Tc and Sort samples (#1-22, paired-end sequencing), 20 ng of 293T RNA was added as a human spike in control. TruSeq RNA Sample Preparation kit V2 (RS-122-2002) was used to construct cDNA libraries