title: DNA-Seq of homo sapiens, HEK293T cells
design: HEK293T cells were seeded in 12-well plates and  transfected using 2 L Lipofectamine 3000 (ThermoFisher Scientific) with 2 g plasmid expressing specific base editor and 500 ng plasmid expressing specific sgRNA.72 hours later, mCherry/GFP-double positive cells were isolated using flow cytometry. Genomic isolation was performed using phenol chloroform method. Targeted PCR was performed and isolated with Universal DNA Purification Kit (TIANGEN BIOTECH, BEIJING,China).Purified PCR products were prepared with NEBNext Ultra II DNA Library Prep Kit. Deep Sequencing was performed in Hiseq X ten at NovelBio Bio-Pharm Technology, Shanghai, China.