title: GSM4817497: MPs_ Ctr_ rep2 [RNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from 5x10e5 FACS sorted p7 MPs was isolated using the RNeasy Micro Kit (Qiagen). RNA quantity and quality was tested on a Qubit®Fluorometer (Invitrogen) and a Bioanalyzer 2100 (Agilent). Sequencing libraries was prepared following Roche's “KAPA stranded mRNA Seq” library preparation protocol. 11 cycles of PCR were used for libraries amplification followed by sequencing with an Illumina HiSeq 4000 system.