title: Bos taurus RNA-seq
design: Raw data (raw  reads) of fastq format were firstly processed through in-house perl scripts.  In this step, clean data(clean reads) were obtained by removing reads  containing adapter, reads containing ploy-N and low quality reads from raw  data. At the same time, Q20, Q30, GC-content and sequence duplication level  of the clean data were calculated. All the downstream analyses were based on  clean data with high quality.