title: Illumina HiSeq 2000 paired end sequencing; TCR repertoire profiling of T cells expressing NK-cell markers
design: TCR repertoire profiling of T cells expressing NK-cell markers
library_construction_protocol: PBMC from donor peripheral blood. Then positive magnetic bead-based isolation (CD4 or CD8 cells) or FACS sorting. Phenol-chloroform extraction followed by ethanol precipitation 5'-RACE cDNA synthesis with UMI introduced with universal adapter followed by 2-step PCR amplification