title: GSM5191943: Sub1_Pit; Oreochromis niloticus; RNA-Seq
library_construction_protocol: Total RNA isolation from pituitaries and brains was performed using miRNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions. RNA quality and quantity were verified using 2100 Bioanalyzer instrument (Agilent Technologies) RNA libraries were prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA)