title: Whole-genome sequence of Escherichia coli ADD183
design: Genomic DNA (gDNA) was extracted from a single bacterial colony cultured in LB broth using ZymoBIOMICS DNA Miniprep kit (Zymo Research). The genomic library was prepared following the Nextera XT DNA Library Prep protocol (Illumina) and sequenced on the Illumina MiniSeq platform (2 x 150 bp paired-end protocol, 300 cycles).