title: NextSeq 550 sequencing; Differential gene expression in WT mice and mice lacking PTPN2 in T cells
design: Differential gene expression in WT mice and mice lacking PTPN2 in T cells
library_construction_protocol: Samples were collected at the end of the experiment from colonic non-tumor and tumor tissue and flash frozen in liquid nitrogen before storage at -80Â°C before further processing. Mice were treated for three cycles consisting of 7 days with 1.5% dextran sodlium sulphate (DSS) in the drinking water, followed by 10 days with normal drinking water, each. On day 1 and day 9 of each cycle the mice were injected with 10mg/kg body weight Asoxymethane (AOM) RNA was extracted using the RNaesy mini kit from Qiagen according to the manufacturer's protocol Preparation of poly(A) enriched, stranded Illumina TruSeq RNA libraries by Microsynth AG (Balach, Switzerland)