title: Illumina HiSeq 4000 sequencing; Single-cell mRNA-seq from a healthy skeletal muscle cell derived from human induced pluripotent stem cell
design: Single-cell mRNA-seq from a healthy skeletal muscle cell derived from human induced pluripotent stem cell
library_construction_protocol: One cryotube (1.7 million cells) of the M398 healthy cell line was thawed in a T75 BioCoat™ Collagen I cultureware (356485, Corning Incorporated) with SKM02 medium (AMSbio). Cells were harvested with trypsin the following day. 1.5 million cells were centrifuged at 300 g for 5 minutes. Cells were resuspended at 1,500 cells/µL in DMEM F-12 with 10% serum and processed according to the 10X Genomics single-cell RNA sequencing protocol.  NA The library was prepared with Chromium™ Single Cell 3' Library & Gel Bead Kit v2.