title: GSM4876356: murine mammary PyMT tumour rep2; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were minced using scalpels, and then single cells were liberated by incubation for 60 mins at 37°C with 1 mg/ml Collagenase I from Clostridium Histolyticum (Sigma-Aldrich) and 0.1 mg/ml Deoxyribonuclease I (AppliChem) in RPMI (Gibco). Released cells were then passed through a 70 μm cell strainer prior to staining for flow cytometry FACs sorting. Sorted cells were processed using standard protocols associated with 10X Genomics Chromium Single Cell 3' Library and Gel Bead Kit V3 10x Single Cell 3' v3 Gene expression