title: GSM4774262: DKO_ATACseq; Mus musculus; ATAC-seq
library_construction_protocol: Samples were processed for ATAC-seq as previously described (Buenrostro et al., 2013; Buenrostro et al., 2015). Tagmentation reaction was performed using 2.5 µl Illumina TDE1 in 50 µl Illumina TD buffer at 37°C for 30 min. The tagmented DNA library was purified using the QIAquick PCR Purification Kit. Library quantification and number of amplification cycles was determined as described (Buenrostro et al., 2015).