title: MiSeq of DNA from Microbes: Manatee Head  Fall 3
design: Microbes were concentrated from spring  water via TFF. The concentrates were then filtered through a 0.22 m Sterivex  filter (Millipore) to capture the microbial fraction and these filters were  cut out of the sterivex and inserted into the Mo Bio PowerSoil_ DNA Isolation  Kit. Microbial DNA was extracted following the manufacturers protocol. The  extracted bacterial DNA underwent amplicon library preparation and Illumina  sequencing of the 16S-V4 rRNA hypervariable region using 16S-V4 515f and 806r  primers on a MiSeq system.