title: A. argentata Flagelliform silk gland 2 3' DGE
design: Library sequencing was described in Chaw et al. (2015). Libraries were sequenced on a HiSeq2500 platform (Illumina) at the University of California, Riverside Institute for Integrative Genome Biology. The RNA-seq libraries (total silk, Cep_R2, Fla_R1, Pyr_R1, and Tub_R1 and R2) were paired-end sequenced for 100 cycles. The 3 DGE libraries (Aci, Agg, Fla, Cep, Maj, Min, Pyr, Tub, with R1 and R2 for each) were single-end sequenced for 50 cycles. The biological replicates of the 3DGE libraries were run in separate lanes, and each library was run twice, generating technical replicates.