title: GSM5348301: CR_H3K27ac_cranial_2; Gallus gallus; OTHER
library_construction_protocol: Neural fold dissections were performed on HH9/HH14 embryos to obtain cranial/trunk neural crest cells (10 embryos/20 neural folds per experiment). Cells were dissociated in Accumax (Innovative Cell Technologies, AM105) cell dissociation solution for 20 min at RT under mild agitation. CUT&RUN experiments were performed as previously described (Skene and Henikoff, 2017). CUT&RUN libraries were prepared using the NEBNext ultra II DNA library prep kit (New England Biolabs E7645) according to the kit's protocol.