title: GSM5346264: perturbseq_gene_4-1; Homo sapiens; OTHER
library_construction_protocol: RNA-Seq: RNA extracted by Zymo Direct-zol RNA miniprep columns; Perturb-Seq: 10x Genomics Chromium 3' Assay v3; CUT&Tag: DNA fragments were isolated using protein A-Tn5 transposase fusion proteins targeted to H3K4me3 antibodies (Millipore 04-745), following protocol in Kaya-Okur et al., 2019; PLAC-Seq: cross-linked cells were treated with MboI, followed by proximity ligation and ChIP using H3K4me3 antibody (Millipore 04-745), see Fang et al., 2016. RNA-Seq: NEBNext Stranded Directional RNA with polyA-selection or rRNA depletion; Perturb-Seq: 10x Genomics Chromium 3' Assay v3; CUT&Tag: See Kaya-Okur et al., 2019; PLAC-Seq: See Fang et al., 2016. RNA-Seq: SE50; Perturb-Seq: PE100; CUT&Tag: PE50; PLAC-Seq: PE150.