title: GSM5346258: RF_liver_rep1; Gallus gallus; RNA-Seq
library_construction_protocol: RNA was extracted from the frozen liver of 3 FF and 3 RF broiler breeder hens using an RNeasy Mini Kit with on-column DNase digestion (Qiagen Inc., Valencia CA) according to manufacturer guidelines. RNA quality was analyzed on an Aligent Bioanalyzer (Aligent Technologies, Santa Clara, CA) and all samples had RIN values > 8. Strand-Specific RNA sequencing library preparation was done in the laboratory of Dr. James Giovannoni at the Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, according to a previously described protocol.