title: GSM5188307: Nrf2 KO CDDO-Me d0 after PHx_3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from snap frozen liver tissue using an RNeasy Mini kit (Qiagen, UK). Ribosomal RNA was removed from 1 µg of total RNA using a Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Illumina, UK). RNA-seq libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, UK) and purified using AMPure XP beads (Beckman Coulter, UK). RNA-Seq was carried out on three lanes of an Illumina HiSeq 4000 at 2x150 bp paired-end sequencing with v1 chemistry.