title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of liver biopsies of eIF6 wild type and eIF6 heterozygous mice
design: RNA-seq of liver biopsies of eIF6 wild type and eIF6 heterozygous mice
library_construction_protocol: All experimental mice were sacrificed and the livers were recovered. Approximately 5 mg of liver tissue from high-fat (HFD) diet mice, both eIF6 wt and eIF6 heterozygous, were homogenized and RNA was purified  Total RNA from liver tissues was purified using mirVanaTM isolation kit (ThermoFisher) according to the manufacturer protocols. RNA quality was assessed using the Agilent RNA Pico 6000 Kit and BioAnalyzer (Agilent Technologies)  Random primed cDNA libraries were constructed from 100 ng of total RNA.