title: GSM4773657: ST with MS-275 at D3 (rep1); Ambystoma mexicanum; RNA-Seq
library_construction_protocol: Tissues from axolotls were subjected to total RNA extraction using TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's instructions. RNA samples were purified using a RNeasy Mini Kit (Qiagen, Hilden, Germany), then quantified on a Qubit 4 fluorometer (Invitrogen, CA, USA) and Qsep100 capillary gel electrophoresis (BiOptic, New Taipei City, Taiwan). Libraries were constructed uing SureSelect Strand Specific RNA Library kit according to the manufacturers