title: GSM4725083: B cells 8hr Unt Brd4 ChIP-1; Mus musculus; ChIP-Seq
library_construction_protocol: RNA-seq: RNA was extracted using Qiagen RNeasy Mini kit and gDNA was removed using Qiagen RNAse-Free DNAse set. RNA-seq: 3'mRNA-sequencing libraries were prepared from 100ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) according to the manufacturers instructions and sequenced on the NextSeq 500 (Illumina). ChIP-seq: Lysates were clarified from sonicated nuclei and Brd4-DNA or H3K27ac-DNA complexes were isolated with antibody. DNA product was purified using Zymo ChIP DNA Clean and Concetrator Kit. ChIP-seq: ChIP-enriched DNA was processed and sequenced using NEBnext Ultra II kit according to manufacturer's instructions on Nextseq500 (Illumina).