title: GSM6205602: satellite cells, wild type-2, Day4; Mus musculus; RNA-Seq
library_construction_protocol: SCs obtained by flow sorting were seeded into 6-well cell culture plates at a density of 2.5x10^5. After 4 days of growth, cells were harvested, added with 1 ml Trizol, snap-frozen in liquid nitrogen, and transported to GeneDenovo Biotechnology Co. Data were extracted and normalized according to the manufacturer's standard protocol. Total RNA was extracted from the collected satellite cell samples, the concentration and purity of the extracted RNA were detected by Nanodrop2000, RNA integrity was detected by agarose gel electrophoresis, and RIN value was determined by Agilent2100. A single library construction requires that the total amount of RNA is ≥1ug, the concentration is ≥35ng/μL, OD260/280≥1.8, and OD260/230≥1.0.