title: GSM3459746: MEF MUT S1; Mus musculus; OTHER
library_construction_protocol: Asynchronously proliferating cells were flow-sorted based on their DNA content (Hoechst 33342) into G1 and S phase fractions (Fig.S6) into ice-cold PBS. Genomic DNA was isolated immediately after flow-sorting with Quick-gDNA™ MiniPrep (Zymo Research). Sequencing libraries were prepared with Illumina TruSeq DNA PCR-Free whole genome sequencing kit and sequenced on NextSeq 550 with 150 cycles High Output kit using paired end protocol. Repli-seq