title: GSM5578259: HEK293T cells, siXRN2_rep1 (small RNA-seq); Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA was extracted using Trizol following manufacturer's protocol Small RNA-seq library preparation was similar to previously described (Su et al. 2019) with addition of spike-in. Briefly, 1 ul (500 ul total volume) of Qiaseq miRNA library QC spike-in (Qiagen #331535) were mixed with 1 ug total RNAs before library preparation. The resulting libraries were size selected for 15-50 nt inserts, and pooled for sequencing on Illumina NextSeq500. For mRNA-seq library preparation, 100 ng total RNA was poly-A selected by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and then converted to libraries by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760) according to the manufacturer's protocol. The resulting libraries were quality checked for concentration and size distribution before pooling for paired-end sequencing on Illumina HiSeq by Novogene.