title: GSM4817904: Stool [8]; Mus musculus; OTHER
library_construction_protocol: Microbial DNAs from 1-3 mg of mouse fecal samples were extracted and purified according to the manufacturer's protocol of EXT3-16S DNA Purification and PCR Amplification Kit of Shoreline Biome (Farmington, CT), and the extended (~2,500 bp) region, which contains 16S rRNA gene, the adjacent Internally Transcribed Spacer (ITS) and part of the 23S gene, was amplified using barcoded primer sets in the same kit. To construct the SMRTbell libraries, all reagents were provided by PacBio (Menlo Park, CA) SMRTbell Template Prep Kit 1.0. Equal molar quantities of the amplicons were pooled. According to a PacBio standard protocol (Amplicon template preparation and sequencing) the damage of the DNA templates was repaired and their ends were blunted. After ligating adapters, the failed libraries were degraded by treating exonucleases. To load the libraries onto Sequel, the polymerase complexes were produced and the sample plate containing the final loading dilution was loaded on the machine. Mutiplexing EXT3-16S DNA libraries