title: GSM4725681: wt-1; Mus musculus; RNA-Seq
library_construction_protocol: Trizol At Embryonic Day E13.5 fetal livers were dissected out of mouse embryos and homogenized by gentle pipetting in 1X PBS + 2% FBS. Cells were strained through a 70μm nylon cell strainer (Thermo Fisher) into 1X PBS + 2% FBS. After staining for appropriate antibodies, cells were sorted by FACS and collected in Trizol solution immediately for RNA-Seq, or purified by EZ-Sep (Cell Signaling technologies #17656) and processed for single cell sequencing using the Chromium V3 single cell kit (10X Genomics). RNA was extracted using ethanol precipitation, except for samples 7, 8, and 9 where we used the Agilent Absolutely RNA Nanoprep kit (#400753) Libraries were constructed using Illumina Truseq stranded RNA kit Single-end second strand RNA-Seq Paired-end single cell sequencing