title: GSM5225724: SCI+Ctrl-14d-01; Mus musculus; RNA-Seq
library_construction_protocol: Spinal cord tissues of 3, 14, 35 DPI were isolated and segments containing injured/injected (parenchymal segments spanning ~2-3 mm from each side of the lesion) were rapidly snap-frozen in liquid nitrogen. Total RNA was isolated from spinal cord tissues using the RNeasy Lipid Tissue Mini kit (Qiagen, #74804) following the manufacturer's protocol. The concentration of the total RNA was determined using Qubit RNA BR Assay Kit (Life Technologies) and quality of the total RNA was determined using the RNA 6000 Nano chip on the 2100 Bioanalyzer automated electrophoresis system (Agilent Technologies). The libraries were prepared using an Illumina MiSeq paired-end kit and sequenced as paired-end, 2x150 bp on the Illumina MiSeq. Total RNA was used for library preparation of each sample, which was subsequently bar-coded and prepared according to the manufacturer's instructions (Illumina). The sequencing run was performed according to the manufacturer's instructions and generated a total of 40 million reads per sample.