title: GSM6205607: Spatial Transcriptomics Sample 1; Mus musculus; OTHER
library_construction_protocol: scRNA-seq: Microdissect, dissociate, and pool cells across acute brain slices in B6 mice; spatial transcriptomics (ST): flash frozen brains, cryosectioning 10um and place on 10xgenomics visium slides, tissue staining for NeuN and DAPI, permeabilization and library construction following manufacturer's protocol scRNA-seq: As in (Erwin*, Bristow*, … , Cembrowski; eLife 2021); ST: as described by 10x genomics for Visium workflow