title: GSM5683178: RCAS_Y_3_S20; Mus musculus; RNA-Seq
library_construction_protocol: DIPG tumors from each group were flash frozen in liquid nitrogen. Total RNA was extracted using the RNeasy midi-kit (Qiagen). The Illumina TruSeq Stranded Total RNA Library Preparation Kit was used to prepare sequencing libraries The procedure includes rRNA depletion, remaining RNA purification and fragmentation, cDNA synthesis, 3' end adenylation, Illumina adapter ligation, library PCR amplification and validation. lllumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50 bp reads.