title: 16S rRNA-Seq of microorganisms: Morca Cave
design: The environmental DNA was extracted from the each cave soil/surface sample using the DNA isolation kit and the The V4-V5 variable region of the 16SrRNA genes from the purified isolated DNA samples were amplified by adding to the bacterial and archaeal specific universal primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-CCGYCAATTYMTTTRAGTTT3).