title: GSM6095953: hAOEC cells - 1 Gy radiation - 72h - set1 [1Gy72h_1_miRNA]; Homo sapiens; miRNA-Seq
library_construction_protocol: Cells were washed lysed directly in Trizol. Phenol-chloroform extraction and total RNA isolation was performed as per manufacturer's guidelines using miRNeasy kit (Qiagen). The small RNA libraries were prepared using the Qiaseq miRNA Library Preparation Kit (Qiagen, Hilden, Germany) following manufacturer's instructions. Briefly, a universal preadenylated DNA adapter is added to the 3' ends and an RNA adapter is ligated to the 5' end. cDNA was synthesized using 3'-adapter as the primer. cDNA was subsequently cleaned up using magnetic beads and libraries were amplified and different 6-nt indices were included for each sample to enable sample identification after multiplexing. Library sizes were assessed using Bioanalyzer and average library sizes were around 180 bp. Samples were pooled and sequenced on Illumina Hiseq X sequencer generating 10 million single end reads per sample.