title: NextSeq 500 sequencing; Generation of progesterone-responsive endometrial fibroblasts from human induced pluripotent stem cells: role of the WNT/CTNNB1 pathway
design: Generation of progesterone-responsive endometrial fibroblasts from human induced pluripotent stem cells: role of the WNT/CTNNB1 pathway
library_construction_protocol: Embryoid bodies (EB) generated from hiPSC were cultured with defined chemical cocktails including WCP activator CHIR99021. Primary endometrial stromal fibroblasts (EMSF) were isolated from endometrial tissue. SUSD+ cells were isolated from primary EMSF using flow cytometry. Embryoid bodies (EB) generated from hiPSC were cultured with defined chemical cocktails including WCP activator CHIR99021. Total RNAs were isolated from hiPSC, D2.5, D4, D6, D8, D14, D22 VC, D22 IVD EB, and primary EMSF using the RNeasy Mini Kit (Qiagen, Germantown, MD).  RNA-seq libraries were constructed from ~500 ng DNAse-treated total RNA using KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems, Wilmington, MA).