title: GSM4996247: 6_h_after_exercise_10; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from the frozen samples (~20 mg) using an RNeasy mini kit (Qiagen, Germany) with DNase I treatment (Fermentas, Lithuania). The RNA concentration and integrity were evaluated in a fluorimeter (Qubit 3.0; Thermo Scientific) and by capillary electrophoresis (Bioanalyzer 2100, Agilent, USA), respectively; all samples were at least 7 in RIN. Libraries were constructed from 2.5-3 mg of RNA according to nAnT-iCAGE (non-Amplified non-Tagging Illumina Cap Analysis of Gene Expression) protocol  using nAnT-iCAGE Library Preparation kit (DNAform, Japan) and SuperScript III Reverse Transcriptase (Invitrogen, USA). The concentration of the obtained libraries was measured on a Qubit 3.0 fluorimeter (Thermo Scintific, USA); the quality of the libraries was checked using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA). The libraries were then validated using real-time PCR (KAPA Library Quantification Kits Illumina, KAPA Biosystems, South Africa) and sequenced