title: GSM5512798: Midgut Wg Rep 2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Flies were dissected in 1 × PBS. RNA was isolated from at least 10 midguts using the Isolate II RNA Mini kit (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific). Library preparation was performed using 1µg of total RNA and sequencing was performed using Illumina HiSeq 4000 System (2 × 151 bp read length, 40 million reads per sample) by NovogeneAIT Genomics (Singapore).