title: GSM5578045: U2_scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: 10X Chromium Spatial Gene Expression Slide & Reagent Kit, 16 rxns PN-1000186 10X Chromium Library Construction Kit, 16 rxns PN-1000190 A Visium Spatial Gene Expression library comprises standard Illumina paired-end constructs which begin and end with P5 and P7. The 16 bp Spatial Barcode and 12bp UMI are encoded in Read 1, while Read 2 is used to sequence the cDNA fragment. I7 and i5 sample index sequences are incorporated. TruSeq Read 1 and TruSeq Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing.