title: GSM4996083: REH FASTKD1 sh2 replicate3; Homo sapiens; RNA-Seq
library_construction_protocol: Three independent RNA extractions from control and two knockdown cell lines (sh-1, sh-2) samples were sequenced. For each sample, 1 µg RNA samples (RIN = 10) were used for libraries preparations with TruSeq Stranded Total RNA (RiboZero Human/Mouse/Rat) Library Prep (Illumina, Cat#RS-122-2201) according to manufacturer's instructions. Each library was quantified on Qubit with Qubit® dsDNA HS Assay Kit (Life Technologies) and the size distribution was examined on the Fragment Analyzer with High Sensitivity NGS Fragment Analysis kit (Agilent). Library prepared from FASTKD1 control and knockdown samples were sequenced on Hiseq 4000 (PE150) platform in Novagene company.