title: Illumina HiSeq 2500 sequencing; Bulk tissue RNA-seq of different sea lamprey (Petromyzon marinus) organs
design: Bulk tissue RNA-seq of different sea lamprey (Petromyzon marinus) organs
library_construction_protocol: Tissue samples were dissected from wild caught specimens. Sampled animals were euthanized by submersion in 0.1% MS-222 (Sigma, A5040-25G), unless specified otherwise, followed by decapitation according to local guidelines.  Total RNA was extracted using TRIzol. Strand-specific RNA-seq libraries were generated using the Illumina TruSeq Stranded mRNA Library protocol.