title: GSM4998001: COV434_Parent_BRG1_RNAseq_Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from frozen cell pellets was extracted using the Zymo Quick-RNA Miniprep Kit (Zymo cat# R1054). Total RNA was prepared using the Illumina TruSeq mRNA v2 kit with an input of 500ng of RNA for each sample to produce unstranded RNA libraries following the manufacturer's protocol. Final RNA libraries were quantified using the Qubit High Sense Reagent kit and Agilent Tapestation HSD1000 tapes. Libraries were equimolar pooled and paired end sequenced across three lanes of a HiSeq4000 (paired end x 78 bp).