title: Metagenome
design: Total genomic DNA from mouse feces was extracted using DNeasy PowerSoil Kit (Qiagen) following the manufacturers instructions as described. Purified DNA was prepared for shotgun metagenomic sequencing using the Nextera XT library preparation method. Libraries were quality assessed using qPCR and a Bioanalyzer (Agilent Technologies) and subsequently sequenced on a NovaSeq 6000 S2 flow cell using a 300 cycle (2150bp) kit.