title: 18S rRNA-seq of hypersaline lake
design: gDNA was extracted from water sample using MP Bio kit then cleaning and concentration of gDNA was done followed by PCR amplification by universal primer for 18s v4 region.Quality and quantity assessment by Qubit Flurometer and Agarose Gel Electrophoresis. Multiplex amplicon lib were prepared on Illumina MiSeq and raw reads were obtained