title: WGS of Rattus Norvegicus: EpCAM+ cells from mammary tumor
design: 1g aliquot of the genomic DNA was prepared using the Illumina TruSeq PCR-free DNA HT sample preparation kit with 450bp insert size.  Intact genomic DNA was sheared using the Covaris sonicator (adaptive focused acoustics), followed by end-repair and bead-based size selection of fragmented molecules.  The size selected fragments had adenines added to the 3 ends of the DNA, then Illumina sequence adaptors ligated on, followed by PCR amplification, final library QC and sequencing on HiSeqXTen at 2 x150bp to depth of 30x