title: GSM5543306: Cell_7d_bio1_tech1; Homo sapiens; OTHER
library_construction_protocol: gDNA was purified from a minimum of 1 × 106 library cells using Blood Genomic DNA Mini Kit (CW2087, Cwbio). Three PCR reactions were performed for each gDNA sample to amplify the p53 region. Each reaction contained a total volume of 50 μL with 1 μg gDNA, 25 μL of VAHTS® HiFi Amplification Mix (N616-02, Vazyme) and 2.5 μL of each (5 μM) library amplification primer. The parameters for PCR were 95 °C for 3 min, 27 cycles of 98 °C for 20 s, 58 °C for 15 s, and 72 °C for 45 s, followed by one cycle of 72 °C for 5 min. Amplicons were separated from nonspecific fragments by electrophoresis on a 1% agarose gel, extracted from the gel and purified using a Wizard® SV Gel and PCR Clean-Up System (A9282, Promega). Sequencing sample preparations reactions were performed using TruePrep DNA Library Prep Kit V2 for Illumina (TD501, Vazyme), followed by sequenced on an Illumina Novaseq6000 (Illumina) using two reads, each 150 bases in length.