title: GSM5888990: ART Patient 310817 Source LN 2 [R1015.S0032]; Homo sapiens; RNA-Seq
library_construction_protocol: Replicate populations of 500-2,500 HIV-specific CD8+ T cells were FACS sorted into RLT Plus lysis buffer supplemented with 1% beta-mercaptoethanol and flash frozen at -80C for storage. Lysates were thawed on ice and RNA was isolated using AllPrep DNA/RNA Micro Kit (Qiagen Cat#80284) per manufacturer's protocol for isolation of total RNA including small RNAs. Whole-transcriptome libraries were prepared using oligo-dT priming and SuperScript II (ThermoFisher Cat#18064022) reverse transcription as per standard SmartSeq2 protocols, then barcoded and pooled for Illumina 76bp paired-end sequencing on a NextSeq 550.