title: GSM5971629: NaAC-Ctr-1; Escherichia coli; RNA-Seq
library_construction_protocol: RNA sequencing of 6 samples was performed using an Illumina HiSeq platform. The samples were collected during the mid-logarithmic phase, quickly frozen in liquid nitrogen and stored at -80 °C. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). The quantity and quality of the isolated RNA were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), Nano Drop (Thermo Fisher Scientific Inc.) and 1% agarose gel electrophoresis. The following library preparation required 1μg of total RNA with an RNA Integrity Number (RIN) value above 6.5. The NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, Ipswich, MA, USA) was used to construct next-generation sequencing libraries. The libraries sequencing was carried out on an Illumina HiSeq platform using a 2×150 paired-end (PE) configuration. RNA libraries were prepared for sequencing using standard Illumina protocols