title: GSM4996723: Bulk_Arcuate organoid cells_100d_C6-5_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: ARCOs were dissociated using a papain-based neural tissue dissociation kit (Miltenyi Biotech). Frozen hypothalamus pieces were dissociated using Nuclei EZ lysis buffer (Sigma) as previously described (Habib et al., 2017). Single-cell libraries were constructed as per the Dropseq protocol (Macosko et al., 2015). Bulk RNAseq libraries were constructed using a modified version of the SMARTseq protocol as previously described (Weng et al., 2017). Single-cell and Bulk RNASeq libraries