title: GSM4817726: Cell2_1; Mus musculus; miRNA-Seq
library_construction_protocol: The small RNA from the MIN6 cells and the MIN6-derived exosomes were isolated using Qiagen's miRNeasy Serum/Plasma Kit 1) Input RNA is first polyadenylated in order to provide a priming sequence for an oligo(dT) primer. 2) cDNA synthesis is primed by the 3' smRNA dT Primer, which incorporates an adapter sequence (green) at the 5' end of each first-strand cDNA molecule. 3) When the MMLV-derived PrimeScript Reverse Transcriptase (RT) reaches the 5' end of each RNA template, it adds non-templated nucleotides which are bound by the SMART smRNA Oligo-enhanced with locked nucleic acid (LNA) technology for greater sensitivity. 4) PrimeScript RT uses the SMART smRNA Oligo as a template for the addition of a second adapter sequence (purple) to the 3' end of each first-strand cDNA molecule. 5) Full-length Illumina adapters are added during PCR amplification. 6) cDNA fragments are sequenced by the read length using sequence by synthesis method on the illumina platform. SMARTer smRNA-Seq Kit for Illumina