title: GSM4875637: WT; Mus musculus; OTHER
library_construction_protocol: Spinal cord of three heterozygous GarsC201R/+ or three wildtype mice, flash frozen and immediately lysed by grinding in 10mM Tris-HCL pH 7.4, 5mM MgCl2, 100mM KCL, 1% NP-40, 2% sodium deoxycholate.The lysates from three animals each were pooled. Isolation of mRNA-bound ribosome complexes, RNase I digestion-derived ribosome-protected fragments (RPFs), RNA-size selection and generation of the cDNA libraries was performed as described (Guo et al, 2010; doi: 10.1038/nature09267 )