title: Illumina NovaSeq 6000 sequencing; RNA-seq of mouse iBAT with local acetate administration
design: RNA-seq of mouse iBAT with local acetate administration
library_construction_protocol: Mice were euthanized by CO2, left or right lobe of iBAT were dissected and snap frozen with liquid nitrogen. Adult mice were kept in room temperature, with chow diet. Acetate or saline were administrated to iBAT with a pump. RNA samples were isolated by Trizol. DNAse treatment were applied.  Ribo-zero truseq kit (illumina) was applied for library construction.