title: GSM5889680: WT_polysome_rep1; Mus musculus; OTHER
library_construction_protocol: Total cytoplasmic RNA or RNA bound by more than 4 ribosomes were extracted using TRIzol LS reagent (Invitogen) following the manufacturer's instructions and then subjected to poly(A) selection by using mRNA purification beads (Ambion, 61006). RNA libraries were prepared with 500 ng RNA according to the manufacturer's protocol (Illumina). The libraries were sequenced in 2 × 100 nt manner on HiSeq 2000 platform (Illumina).