title: GSM4996851: a2KO_PA2_E100_3; Mus musculus; RNA-Seq
library_construction_protocol: Pharyngeal arches 1 and 2 were manually dissected from embryos in ice-cold PBS and flash frozen in liquid nitrogen. RNA was extracted using TRIzol and purified using the Zymo Research Direct-zol mini-prep kit (Cat#R2052). Libraries were prepared using the Takara Clontech SMART-seq v4 ultra low input RNA kit (Cat#634891) to generate cDNA, and Illumina Nextera XT Library prep kit (Cat#FC-131-1096) and Illumina Nextera XT Index kit (Cat#FC-131-2001) for library construction according to standard protocols.