title: GSM5348310: RNA_highWNT_TGFB_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation and RNA-seq library preparation was performed on 500,000 hiNCCs after Day 5 of induction. RNA was isolated using the RNAqueous-micro total RNA isolation kit (Thermo Fisher Scientific AM1931) according to the kit's protocol. RNA was poly(A) selected using the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs E7490). TruSeq-barcoded RNA-seq libraries were generated with the NEBNext Ultra II directional RNA library prep kit (New England Biolabs E7760).