title: GSM5225798: RY_LP_R2; Stylosanthes guianensis; RNA-Seq
library_construction_protocol: Total RNA from leaves of stylo with 0 and 250 μM P treatments were extracted using TRIzol Reagent (Invitrogen, Carlsbad, USA) according to the manufacturer's protocol. RNA integrity and concentration were determined using an Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, California, USA). Oligo (dT) magnetic beads are used to select mRNA with polyA tail. RNA libraries were prepared for sequencing using standard Illumina protocols. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, USA)