title: GSM3460526: p53_si_TGFB_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from MEC1 cells was extracted using TRIzol (Thermo Fisher Scientific). Total RNA was enriched for poly(A) RNA and fragmented for library construction. RNA fragments were reverse transcribed followed by A-tailing and and index adapter ligation, then denatured and amplified on cBot. Library analysis and quality checks were performed on a LabChip GX.