title: GSM5893347: pDC_TRIM33_2; Mus musculus; ChIP-Seq
library_construction_protocol: Splenic cDC1s (CD11c+ SiglecH- MHC II+ CD24+ CD172a-), cDC2s (CD11c+ SiglecH- MHC II+ CD24- CD172a+) and bone marrow pDCs (CD11b- CD19- CD11c+ SiglecH+ PDCA-1+) of B16FL-treated mice were isolated by fluorescent activated cell sorting.  ChIP samples were prepared with the ChIP-IT® Express Enzymatic Shearing Kit (#53035, Active Motif) after cross-linking cells with 1 % formaldehyde at room temperature for 10 min and quenching with 0.125 M glycine. For each sample, chromatin from 5 × 10^6 sonicated and enzyme-sheared cells was incubated with 5 µg anti-TRIM33 antibody (#A301-059A, Bethyl Laboratories) at 4 ℃ overnight. Magna ChIP™ Protein A+G magnetic beads (#16-663, Millipore, Temecula, CA, USA) and MinElute PCR purification kit (#28004, Qiagen, Germantown, MD, USA) were used to retrieve immunoprecipitated DNA. Annoroad Gene Technology (Beijing, China) constructed the sequencing libraries using standard Illumina protocols.