title: GSM3460507: RNA-Seq_X1_sm2_exp2_pe rep3; Schmidtea mediterranea; RNA-Seq
library_construction_protocol: Total RNA was extracted from 100 000 FACS-sorted cells  using TriFast reagent  (peqlab) according to manufacturer extraction. Small RNA (< 200 nt) was discarded from total extracted RNA with Zymo-spin IC columns (RNA Clean and Concentrator-5, Zymo Research). Next, rRNA was depleted using custom DNA probes against planarian rRNA (as described in the manuscript). RNA libraries from 100,000 FACS-sorted planarian X1 and Xins cells were constructed as described (Zhang et al., 2012).