title: GSM5776106: A2_Hyp WT 2; Mus musculus; RNA-Seq
library_construction_protocol: Lungs from 5 day-old pups were isolated and immediately cryopreserved in 10% DMSO and 90% FCS at -80°C. Samples were thawed and stained by flow cytometry to sort for endothelial, epithelial and immune cells. 8 single cell RNA libraries were constructed using a Chromium controller (10X Genomics Chromium Sinlge Cell 3' V3.1 chemistry). A total of 12 cDNA amplification cycles and 15 cycles of library amplification were used. Sequencing was carried out using an Illumina Novaseq 6000 SP Reagent Kit v1.5 (100 cycles)