title: GSM5543285: diabetic subject 8; Homo sapiens; RNA-Seq
library_construction_protocol: Bone fragments homogenized in a bone mill, in Trizol in a bullet blender, and RNA purified with RNaEasy (Qiagen) Ribosomal RNA was removed via Epicentre Ribo-Zero Gold Kit. The ribo-minus RNA was purified and then fragmented using divalent ions under elevated temperatures. The RNA fragments were reverse transcribed to form the final cDNA library via deoxyuridine triphosphate (dUTP).