title: GSM5191750: IgG_sequence; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were lysed in an ice-cold lysis buffer. Then, RNA immunoprecipitation (RIP) was performed as described (Zhao et al., 2008). RNA extracted using Trizol by following manufacturer's instruction (Invitrogen). rRNAs were removed from the immunoprecipitated RNA and input RNA samples by using Ribo-Zero™ rRNA Removal Kit (Illumina, San Diego, CA,  USA). RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA,  USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). 10 pM libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on Illumina NovaSeq Sequencer according to the manufacturer's instructions.