title: HiSeq X Ten paired end sequencing; Primary monocyte to macrophage differentiation involves significant changes in chromosome conformation different from cultured THP-1 cells
design: Primary monocyte to macrophage differentiation involves significant changes in chromosome conformation different from cultured THP-1 cells
library_construction_protocol: Monocytes were isolated from peripheral blood of healthy volunteers and purified with  EasySepTM Human CD14 Positive Selection Kit II(STEM CELL,Catalog #17858). Macrophages were induced from monocyes with GM-CSF for 7 days. Total RNA was extracted with Trizol (Thermo Fisher Scientific) and then treated with DNase to avoid genomic DNA contamination. mRNA isolation was performed with NEBNext PolyA mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA). The RNA-Seq library was prepared with KAPA Stranded RNA-Seq Kit for Illumina (KAPA Biosystems, Wilmington, MA)