title: GSM4996873: vegetative sample 2; Selaginella nipponica; RNA-Seq
library_construction_protocol: Total RNA was isolated with CTAB method purified mRNA by virtue of oligo (dT) was fragmented into 100 – 400 bp long with divalent cations. Random hexamer primers were used to synthesize the first-strand cDNA, and Illumina paired-end adapters were then ligated onto the both ends of the double-strand cDNA, which would produce 200 ± 25 bp molecules that immediately underwent gel purification. Sequencing was conducted on the Illumina HiSeq2000 or 4000 platform in Beijing Genomics Institute (BGI) - Shenzhen (Shenzhen, China, http://www,genomics.cn/index.php). Both forward and reverse strands were analyzed using a sequencing-by-synthesis method.