title: 16S rRNA of fecal: human
design: DNA was extracted from 200mg fecal using the QIAamp DNA stool mini kit. The V3-V4 regions of the bacterial 16S rRNA genes were amplified from each DNA sample using the F3 (5-CCTACGGGNBGCASCAG-3) and R4 (5-GACTACNVGGGTATCTAATCC-81) primers.