title: MiSeq of DNA from  Microbes: Manatee Head Fall 1
design: Microbes were  concentrated from spring water via TFF. The concentrates were then filtered  through a 0.22 m Sterivex filter (Millipore) to capture the microbial  fraction and these filters were cut out of the sterivex and inserted into the  Mo Bio PowerSoil_ DNA Isolation Kit. Microbial DNA was extracted following  the manufacturers protocol. The extracted bacterial DNA underwent amplicon  library preparation and Illumina sequencing of the 16S-V4 rRNA hypervariable  region using 16S-V4 515f and 806r primers on a MiSeq system.