title: GSM5225858: HTO; Homo sapiens; OTHER
library_construction_protocol: For ECCITE-seq, we ran one lane of a 10x Genomics 5' kit (Chromium Single Cell Immune Profiling Solution v1.0, 1000014, 1000020, 1000151). Cell hashing was performed using four hashtag-derived oligonucleotides (HTOs). Gene expression (cDNA), hashtags (HTOs) and guide RNA (Guide-derived oligos, GDOs) libraries were constructed by following 10x Genomics and ECCITE-seq protocols. We sequenced the cDNA, HTO and GDO libraries with two NextSeq 500 1x75 high-output runs (Illumina). ECCITE-seq sequencing strategy, with the following read recipe: R1 = 26; i7 = 8; R2 = 57