title: GSM5578177: Single cell RNAseq Presbyosmic 1; Homo sapiens; RNA-Seq
library_construction_protocol: Biopsy specimens were digested using collagenase I, dispase and DNAse, then further digested using papain and trypsin. Cells were filtered through a 70 μm strainer, pelleted and washed, and then treated with an erythrocyte lysis buffer and washed again. The cells were then resuspended in PBS with 0.1% bovine serum albumin and 0.2% anti-clumping agent (Invitrogen) and immediately processed for scRNA-seq. Samples were processed for scRNA-seq using Chromium Single Cell 3′ Paired-End, Dual-Index Library & Gel Bead Kit v3.1 (10X Genomics) targeting 5000-10000 cells, then sequenced using NextSeq 150bp (Illumina) flow cells.