title: GSM4724984: 128651.tumor.H3K27ac; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin extracted from formalin-fixed cells was fragmented with a Branson 250 sonifier, solubilized and immunoprecipitated with H3K27ac antibody (Active Motif, Inc., Carlsbad, CA) overnight at 4°C. Antibody-chromatin complexes were pulled down with protein G-Dynabeads (Life Technologies), washed, and eluted. Immunoprecipitated DNA was extracted with AMP Pure beads (Beckman Coulter) after crosslink reversal, RNase A, and proteinase K treatment. ChIP DNA was quantified with Qubit.