title: GSM6206445: mouse embryonic fibroblasts, con, 48h; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA library preparation was performed as described in the QIAseq Stranded RNA Library Kits (QIAGEN). Total RNA was extracted from mice tissuescells. The mRNA was fragmented to an average insert size of 200–400 bp. The cleaved RNA fragments were copied into first-strand cDNA using reverse transcriptase (Thermo Fisher Scientific) and random primers. The first-strand cDNA was converted into double-stranded DNA in the presence of dUTP. These cDNA fragments were subjected to the addition of a single 'A' base and subsequent ligation of the adapter. The products were purified and enriched via PCR to generate the final library.