title: GSM4996769: a2KO_PA2; Mus musculus; RNA-Seq
library_construction_protocol: Pharyngeal arches 1 and 2 were manually dissected from 6 wildtype and 5 Hoxa2-/- embryos in ice-cold PBS. Individual PAs were pooled to produce a total of four samples (WT PA1, WT PA2, a2KO PA1, and a2KO PA2). Pooled samples were dissociated in 0.25% Trypsin + EDTA with manual disruption by pipetting to a single-cell suspension. The reaction was stopped with fetal bovine serum and samples were washed in DPBS. The samples were then loaded on a 10X Chromium Single Cell Controller to capture single cells. Libraries were prepared using the Chromium Single Cell 3' Reagents Kit v3 (Cat# CG000183 Rev A).