title: DNA-Seq of fast food: Prokaryotic diversity contaminants of fast foods
design: DNA was isolated from selected fast foods and environmental samples using phenol-chloroform method and then the Total DNA was shipped for sequencing variable region V4-V7 was amplified barcoded primers 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) .PCR amplification was carried out in 30 cycles using HotStarTaq Plus Master Mix Kit (Qiagen, USA) under following conditions.