title: GSM4774500: ChIP_mESC_2i_Kdm2b_rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies. ChIP DNA library was constructed for HiSeq4000 (Illumina) sequencing using NEBNext UltraII DNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer's instructions. Adapter-ligated DNA was amplified by PCR for 12-14 cycles and followed by size selection using agarose gel electrophoresis. The DNA was purified using QIAquick gel extraction kit (Qiagen) and quantified both with an Agilent Bioanalyzer and Invitrogen Qubit. The DNA was diluted to a working concentration of 20nM prior to sequencing. Sequencing on an Illumina HiSeq4000 instrument was carried out by the Genomics Core Facility at Michigan State University.