title: GSM4722075: AMs_lung_Lcn2KO_5; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were digested using DNAse I and Collagenase I. AMs were isolated using FACS. For each sample, 200 cells were sorted into 96-well plates containing 4 μl cell lysis buffer (nuclease-free H2O [Life Technologies] with 0.2% Triton X-100 [Sigma] and RNase Inhibitor [2 U/µl, Takara/Clonentech]). Cell lysates were stored at -80°C until library preparation according to the Smart-Seq2 protocol (Picelli et al, 2014). Libraries were constructed using the Nextera XT DNA Sample Preparation Kit with 1ng of cDNA.