title: GSM5171943: donor_3_unstim_LPS; Homo sapiens; RNA-Seq
library_construction_protocol: Dendritic cells were harvested and washed with ice-cold PBS after 4 h of dendrimer stimulation, and pooled to obtain sufficient amounts of RNA. Total RNA was extracted with a standard TRIzol isolation protocol (Thermo Fisher Scientific, cat#15596018). To prepare the mRNA library, 2 μg RNA per sample was used. The library was synthesized using the TruSeq® Stranded mRNA Sample preparation kit (Illumina , cat# RS-122-9004), according to manufacturer's LS protocol.