title: 16SRNA:aquatic metagenome
design: For the study, water samples were taken at the sites of annual Vibrio cholerae isolation in the Trans-Baikal Territory. In the Trans-Baikal Territory, water samples were studied from stationary sampling points for V. cholerae on Lake Kenon, the Chita and Borzya rivers. Samples were taken from the surface near the coast in the most stagnant and warmed areas in sterile 1 L bottles. The material was concentrated through a system of sterile polycarbonate filters with pore diameters of 5, 1.2, and 0.22 m. Then total DNA was isolated. Microbial communities were characterized using Illumina MiSeq with amplifications of V3-V4 hyper-variable region of 16S