title: GSM3457491: Total_Brain_wDahT_2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Isolation of RNA from frozen tissue samples of all genotypes (wDah control and  dilp2-3,5 flies, both in the presences and absence of Wolbachia) was performed on three independent biological replicates using Trizol Reagent (Thermo Fisher Scientific) according to the manufacturer's instructions, followed by DNase treatment (Qiagen). RNA quality was determined using the BioRad Experion (BioRad). RNA-seq library preparation and sequencing was performed by the Max Planck-Genome-centre Cologne, Germany (http://mpgc.mpipz.mpg.de/home/). Stranded TruSeq RNA-seq library preparation was performed on 2 μg of total RNA after rRNA depletion (Ribo-zero). Brain samples were treated as above, however total RNA input was reduced (500ng). RNA libraries were prepared for sequencing using standard Illumina protocols