title: GSM5191853: HDF_DA_48h_800M; Homo sapiens; OTHER
library_construction_protocol: After the irradiation or further incubation, genomic DNA was isolated using Quick-DNA Miniprep Plus Kit (Zymo Research; Irvine, CA) according to the manufacturer's instruction manual. Genomic DNAs were framgmeted to an averge length of 300 bp and circuliarized with T4 DNA ligase at 16°C overnight. Non-circularized linear DNA were removed with Plasmid-Safe ATP-Dependent DNAse treatment. To generate DNA double-strand breaks at CPD lesions, the circularized DNA was treated with T4-PDG and APE1 followed by E. coli phrB photolyase, and then S1 nuclease to create double-strand breaks with ligatable ends. To generate double-strand breaks at deaminated CPD sites, the circularized DNA was initially treated with E. coli phrB photolyase and followed by UDG (NEB) and APE1 treatment, and then S1 nuclease to create double-strand breaks with ligatable ends. A-tailed with Klenow fragment exo- (NEB) and then adaptor was ligated with NEBNext Ultra II Ligation Master Mix and followed by incubation with USER enzyme (NEB). The purified DNA libraries were amplified by PCR with NEBNext Ultra II Q5 Master mix and NEBNext i5 and i7 primers and used for DNA sequencing.