title: GSM5542643: NTC_rep1; Mus musculus; RNA-Seq
library_construction_protocol: SSC samples were collected after 18 days cultured in different treatment. Total RNA isolation and cDNA libraries generation were performed according to the manufacturer's instructions of NEB Next Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760L). After purification of amplified cDNA products, the NGS libraries were prepared with the following procedures including end repair, adaptor ligation, size selection, PCR amplification, library purification and quality control. The constructed NGS libraries were sequenced with Illumina Hiseq XTEN platform at Novogene and sequencing strategy was paired-end 150 bp (PE150).