title: GSM4875997: HAEC, PS, 4hr, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Samples for RNA sequencing analysis were collected at 1, 4, and 24 hrs after exposure to shear. Samples for RNA sequencing analysis were collected at 0 hr for the static condition. The total RNA from HAECs were extracted by using TRizol RNA isolation reagent (Thermo Fisher, Cat#15596026). The RNA quality was assessed by RNA Integrity Numbers (RIN), using an Agilent Bioanalyzer, and all RNA samples used in this study had a RIN over 9. The RNAs were ribo-depleted, fragmented, and random hexamer primed using Illumina TruSeq stranded mRNA sample preparation kit. Constructed cDNA library was subjected to single-ended 75-bp sequencing on Illumina Hi-seq 4000 instrument.