title: GSM5191868: Decidua Normal 1; Homo sapiens; RNA-Seq
library_construction_protocol: dMCs were washed in complete medium. Cells were then counted and distributed at approximately 1×106 cells/mL in 200 μL FACS wash (FW, 1×PBS, 2% FCS). Cells were stained with CD14 and CD45 antibody for 15 min at 4 °C. Viability was assessed using DAPI staining incubated for 5 min at 4 °C. Cells were then analyzed on a LSR Fortessa (BD Biosciences). Macrophages were gated on as live CD14+CD45+ lymphocytes.