title: GSM4725142: MeDIP-Seq Tet2Rank–/–; Tet3aRank+/– osteoclasts rep1; Mus musculus; MeDIP-Seq
library_construction_protocol: Genomic DNA was prepared from cultured cells using the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich) according to the manufacturers' instructions. Genomic DNA was sonicated to 150 bp fragments with Covaris (Covaris). Methylated DNA was enriched using MEDIP Kit (Active Motif) according to manufacturer's protocol. The illumina libraries were prepared using KAPA Hyper Prep Kit (Kapa Biosystems) and TruSeq DNA UD Indexes (Illumina) according to manufacturer's instructions. Paired-end sequencing (100 bp x 2) was performed using HiSeq3000 (Illumina).