title: GSM5392995: SHR30 carotid body right; Rattus norvegicus; RNA-Seq
library_construction_protocol: Animals were euthanised with pentobarbital sodium (euthatal) and intracardially perfused with ice-cold PBS. Carotid bodies were excised from the common carotid artery bifurcation under the dissecting microscope and immediately snap-frozen in liquid nitrogen. Tissue was homogenized using QIAzol lysis reagent. Total RNA was extracted using phenol:chloroform phase separation method and isolated using ZYMO direct-zol RNA microprep kit following manufacturers instructions. Illumina TruSeq® Stranded mRNA library preparation kit (20020594, Illumina)