title: GSM4774791: 31+3R; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated by phenol-chlorophorm-isoamylalcohol extraction and LiCl precipitation. RNA samples were further purified with TURBO DNA-freeTM Kit (Thermo Fisher Scientific, Waltham, Massachusetts, USA) according to the manufacturer's instruction. RNA integrity number (RINe) of samples (2 µg; 50-200 ng/µl; OD260/280 = 1.8-2.1; OD260/230 > 1.5) were determined in a pre-control of RNA quality. TruSeq Stranded mRNA Library Prep assay