title: GSM4725516: RNA sequencing of WT pir1-WT cells 2; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: RNA was extracted using MasterPure Yeast RNA purification kit (MPY03100, Lucigen) according to manufacturer's recommendation. ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) or Ultra II Directional RNA Library Prep Kit for Illumina (NEB) was used according to manufacturer's recommendation. Libraries were analyzed using an Agilent 2100 BioAnalyzer (Agilent) and sequenced on the Illumina MiSeq Platform or Illumina NextSeq 500 platform.