title: Transcriptome profiling in flower bud development in walnut
design: Female flower buds were collected from the upper bud of the axillary bud at the second and third positions below the terminal top bud. The flower buds were collected at five stages (designated as P01, P02, P03, P04, and P05) at 20-day intervals starting from the initial period of flowering (P01). Gene transcription during these stages was further studied through PacBio Iso-Seq and RNA-seq analyses.