title: GSM4876023: Sample 10 OAC TRG 4; Homo sapiens; miRNA-Seq
library_construction_protocol: Extraction of miRNA from small EVs was performed using the commercial miRNeasy Serum/Plasma kit (QIAGEN, #217184) according to the manufacturer's protocol.  Five microlitres (0.1 picomole) of each of the synthetic RNA molecules ath-miR-159a and cel-miR-54 (Shanghai Genepharma Co.Ltd.) were added to the 500 µl QIAzol vesicle lysate before further processing. Twenty-four µl of RNase-free ultrapure water was used for the final RNA elution step. The library preparation was done using the QIAseq miRNA Library Kit (QIAGEN). A total of 5µL total RNA was converted into miRNA NGS libraries. Adapters containing unique molecular identifiers were ligated to the RNA. Then RNA was converted to cDNA, and the cDNA was amplified, with the addition of indices, using 22 cycles of PCR. The samples were then purified, and library preparation QC was performed using a Bioanalyzer 2100 (Agilent). The libraries were pooled in equimolar ratios based on the quality of the inserts and the concentration measurements.