title: GSM4724428: Control_3: Human epidermal stem cells-control-3; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol was used to extract total RNA, following the manufacturer's instructions. Libraries were prepared according to Illumina's instructions. Briefly, after the sample is qualified, use magnetic beads with Oligo (dT) to enrich eukaryotic cell mRNA. After the enrichment is complete, add fragmentation buffer to break the mRNA into short fragments. Subsequently, six-base random primers (random hexamers) were used to reverse transcription to synthesize double-stranded cDNA using mRNA as a template. Then use AMPure XP beads to purify double-stranded cDNA. Perform end repair on the purified double-stranded cDNA, add an A tail, and connect a sequencing adapter. The double-stranded cDNA fragment size is selected by AMPure XP beads, and finally the selected double-stranded cDNA is PCR enriched to construct a cDNA library.