title: GSM3461217: Th1 rep cells; Mus musculus; RNA-Seq
library_construction_protocol: Unless stated otherwise, all kits were used according to the manufacturer's recommendations. Total RNA was isolated using ZR RNA MiniPrepTM kit  according to the manufacturers instruction. Total RNA concentration and quality of each sample were determined with the NanoDrop ND-Lite (peqlab, Erlangen, Germany) and Qubit fluorometer (ThermoFisher Scientific, Darmstadt, Germany). The “TruSeq Stranded Total RNA library PrepKit with Ribo-Zero” kit (Illumina, San Diego, CA, USA) was used to prepare cDNA libraries for total RNA sequencing from a minimum of 100ng of total RNA isolated from the Th1 rep cells. RNA quality was assessed on a bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Waldbronn, Germany) and the RNA had a  RIN >8 and was considered for further processing.  After quality control (High sensitivity DNA Kit, Agilent Technologies, Waldbronn, Germany). The final cDNA libraries were quality checked (High sensitivity DNA Kit, Agilent Technologies, Waldbronn, Germany), quantified (Qubit dsDNA HS Assay Kit, Invitrogen, Darmstadt, Germany) and were paired-end (75 bp) sequenced on a NextSeq500 Illumina Next Generation Sequencing Device (Illumina, San Diego, CA, USA).