title: RNA-seq of Candida albicans sir2-/- mutant: replicate 1
design: RNA was extracted from exponential cultures using a yeast RNA extraction kit. RNA quality was checked by electrophoresis under denaturing conditions and concentration was measured using a NanoDrop ND-1000 spectrophotometer. Strand-specific cDNA Illumina barcoded libraries were generated from 1 g of total RNA and sequenced as 50bp single end reads using an Illumina HiSeq2000 sequencer.