title: GSM5696505: CD_1h_post_stress_2; Salmo salar; RNA-Seq
library_construction_protocol: Gill samples from CD and LAN4 groups (3 fish per diet and sampling time: initial control without stress, 1h post-stress and 6h post-stress) were used to determine the gene expression by RNA sequencing (RNA-seq). Briefly, total RNA was extracted using the RNeasy Mini Kit (Qiagen) following the supplier's instructions and then, each RNA sample was quantified using a NanoDrop TM 8000 spectrophotometer (Nanodrop Technologies). RNA integrity was measured by Agilent Bioanalyzer 2100 (all samples showed a RNA integrity number ≥ 7) Library preparation and RNA-sequencing were performed by the Norwegian Sequencing Center, using TruSeq Stranded mRNA library prep and Illumina Novaseq 6000 System (150 bp paired-end)