title: Lepidochelys olivacea gonad 26C stage23 then 33C 2hrs replicate4 RNAseq
design: For the incubation temperature shift experiment, we extracted RNA from pools of ten gonads, developmental stage 23, for each new incubation temperature, for each switching time (2, 8, and 24 hours) and each of the biological replicates. RNA was extracted using the Quiagen RNeasy Mini Kit (#74104). We generated strand-specific RNA-seq libraries using the Illumina TruSeq Stranded mRNA Library protocol.