title: GSM5683138: NORAD Arsenite Control replicate1; Homo sapiens; OTHER
library_construction_protocol: Cells were lysed using RNeasy lysis buffer (QIAGEN) supplemented with DTT and proteins were degraded using proteinase K (NEB). Total cellular RNA was purified using the RNeasy maxi kit (QIAGEN) and quantified using Qubit RNA BR assay kit.  Library preparation was done as described in (Ziv et al., 2018, doi.org/10.1038/s41592-018-0121-0), using 6N unique molecular identifiers to eliminate PCR biases. Pre-adenylated adapters were used and all ligation reactions were carried without ATP to reduce ligation artifacts. Libraries were PCR amplified using KAPA HiFi HotStart Ready Mix (KAPA Biosystems). PCR products were size-selected on a 1.8% agarose gel before loading on a HiSeq 1500 sequencer (Illumina) for paired-end 100nt runs