title: WGS Staphylococcus aureus: Lower respiratory track mechanically-ventilated patients
design: Sample grown overnight in 2 mL TSB and centrifuged at 5000 x g (5 min). Pellet suspended and incubated 37C (1h) and then with RNAase A (1h at 56C). DNA mini and Blood mini kit (Qiagen 51304) employed for gDNA preparation. Genome sequencing on an Illumina HiSeq4000 (paired-end) was done in Wellcome Centre for Human Genetics (WHG) (University of Oxford).