title: WGS of poplar (Populus x sibirica): mature male tree
design: DNA was fragmented on an S220 ultrasonic homogenizer (Covaris, USA), and 1 g of fragmented DNA was used to prepare the library using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK) with a size selection of adaptor-ligated DNA of about 600 bp.