title: 16S rRNA of mangrove soil: PC
design: amplification of bacterial 16S rRNA V3-V4 region by PCR was performed as Library size was verified on an Agilent DNA1000 chip and cleaned using AxyPrep Mag PCR clean-up beads (Axygen Biosciences, Union City, CA). Illumina dual indexes and sequencing adapters were added Illumina dual indexes and sequencing adapters were added The PCR products were cleaned using AxyPrep Mag PCR clean-up beads (Axygen Biosciences, City, Country). The quality of the libraries was assessed on an Agilent DNA1000 chip with the Qubit dsDNA HS Assay Kit. Sequencing was performed using the Illumina MiSeq (v3) sequencing cartridge to produce paired-end libraries of 300 bp read-length.