title: STC108_D7
design: Sputum samples from patients were directly subjected to metagenomic DNA sequencing using the molzym kit, which degrades eukaryotic DNA from the specimens. The extracted prokaryotic DNA was quantified using Nanodrop and amplified using PCR as part of the Nanopore library preparation protocol. The amplified libraries were run on the MinIon after pooling 12 sputum samples together into one batch, using unique sequencing adaptors. The sequencing run took 48 hours to obtain a coverage of between 5X and 10X. The resulting fast5 files were then converted into fastq files for downstream analyses.