title: RNA-seq of LEC2_LO_1_leaf
design: For mRNA-seq analysis, total RNA (10 _g) for each sample was used to purify polyA+ mRNA that was used for synthesis and amplification of cDNA. The RNA-seq libraries were prepared using TruSeq RNA Sample Preparation Kit from Illumina (San Diego, CA). RNA libraries were sequenced on an Illumina HiSeq2000 instrument (100 bp single-end)