title: Lepidochelys olivacea brain 26C stage25 replicate2 RNAseq
design: We extracted RNA from pools of embryos or embryo tissues. For each developmental stage (21, 23, 25, 26), for each of the two incubation temperatures (26C -male-producing temperature- and 33C -female-producing temperature) and each biological replicate, we pooled together RNA from three embryos (stage 21), ten gonads, three brains, three mesonephros, and two livers (stages 23, 25, and 26). RNA was extracted using the Quiagen RNeasy Mini Kit (#74104). We generated strand-specific RNA-seq libraries using the Illumina TruSeq Stranded mRNA Library protocol.