title: Bisulfite-seq of ROxcontrol_2_Neg
design: Bisulfite libraries were prepared from 100 ng genomic DNA using the TruSeq Nano kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions, with the following modification. After adapter ligation and cleanup, samples were bisulfite-treated using the Epitect Plus kit (Qiagen, Hilden, Germany). Thermal cycle incubation was repeated once before clean-up. DNA fragments were amplified using the Kapa HiFi Hotstart Uracil+ PCR mix in 14 cycles of PCR (Kapa Biosystems, Wilmington, MA, USA). Bisulfite libraries were sequenced on an Illumina HiSeq3000 instrument (150 bp paired-end reads).