title: GSM5696416: IL17F_D3; Homo sapiens; ChIP-Seq
library_construction_protocol: The formaldehyde fixed and FACS sorted Th17 cells (~100k) were lysed in ChIP lysis buffer and sonicated until the DNA fragment length sheared to the range of 200-600 bp. The supernatant containing the soluble sonicated chromatin was collected by centrifugation. NEBNext ultra II DNA library prep kit (NEB) was used to prepare the illumina sequencing libraries, following the manufacturer guidelines. The DNA libraries were dual indexed with NEBNext multiplex oligos (NEB) and were sequenced (single end) on Illumina Miseq platform. For each sample, approximately 30 million reads were sequenced