title: GSM6098357: Lung, P8, Cxcl12+ sort; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were removed, minced and processed into a single cell suspension in a Collagenase I (8 mg/5ml) /Dispase (1:10)/RNase-free DNase (1:500) solution for 20 min at 37˚C. CD31+TdTomato+ cells underwent FACS-based isolation from single cell suspension using a MoFlo Astrios EQ (Beckman Coulter) flow cytometer with antibody staining for CD31-PECy7, CD45-APC, and EpCAM- FITC. Following negative selection for CD45 and EpCAM, CD31+/TdTomato+ ECs and CD31-/TdTomato+ mesenchymal-enriched cells were collected in FACS buffer without EDTA. Cells were resuspended as per the 10X Genomics recommended protocol, and 16,500 cells were loaded onto the 10X Chromium platform, to target a recovery of 10000 cells. The libraries were prepared according to the manufacturer's protocol using 10x Single cell 3' v3 chemistry.