title: GSM4725015: THP1-input_rep2; Homo sapiens; OTHER
library_construction_protocol: THP1 cells (80 million) were fixed with glutaraldehyde for 30 min, washed and lysed in ChIRP lysis buffer containing SUPERase•In and protease inhibitors. Lysates were sonicated in Bioruptor to fragment DNA (200-1000 bp) and diluted with hybridization buffer (1:2). Then DRAIR tiling biotinylated oligonucleotides (Stellaris, Novato, CA) were added (10 pg/ml) and incubated overnight at 37 °C. The following day, ChIRP complexes were captured using streptavidin-magnetic beads and washed 5 times with wash buffer, and ChIRP-RNA and ChIRP-DNA were isolated from the beads. ChIRP-DNA was used for DNA-seq on Illumina platform. Library construction and Data analysis to identify DRAIR chromatin binding sites was performed following published protocols (Chu et el, Molecular cell. 2011;44(4):667-78). Enriched and input DNA samples were converted to Illunina libraries using Kapa DNA library prep kit. Libraries were sequenced with Hiseq 2500 following manufacturer's recommendations. ChIRP-Seq (Chu et al, Molecular cell. 2011;44(4):667-78)