title: GSM5191883: rdr5_Bud_smRNA_Seq_14129; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: 30 days unopened flower buds was freezed by liquid nitrogen, and RNA was extracted by Quiazol Sequencing libraries were prepared for smRNA-seq and RNA-seq as described previously [1]. For gel-mediated size selection for library inserts, small RNAs (15 – 50 nt) were selected using high percentage (15%), denaturing acrylamide gels and following a limited RNA fragmentation reaction 140 – 250 nt RNA fragments were purified using acrylamide gels for total RNA samples that were purified from Arabidopsis 30-day unopened flower bud