title: GSM5543388: CD CT8-1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from freshly picked mouse islets or transfected INS-1 832/13 cells using the RNeasy mini kit (Qiagen, Hilden, Germany). RNA size and quality were assessed using the Tape Station Bionalyzer (Agilent, Santa Clara, CA), and concentration was determined by Qubit fluorometry. RNA libraries were prepared using 100 ng of total RNA. RNA libraries were prepared using polyA selection method (Illumina, San Diego, CA) according to manufacturer's instructions.