title: GSM4817330: Naive #4; Rattus norvegicus; miRNA-Seq
library_construction_protocol: Whole blood was drawn from the lateral tail-vein under isoflurane anesthesia, into Microtainer K2 EDTA-tubes. The blood samples were thoroughly mixed with EDTA and placed on ice. Centrifugation was performed immediately at 1300g for 10 min (+4°C) to obtain plasma. RNA was isolated from 200 µL plasma using the miRNeasy serum/plasma kit. Small RNA libraries were prepared with QIASeq miRNA library kit for Illumina NGS systems. Small RNA-Seq. Single-end sequencing of 75 bp reads performed at a depth of 12M, with one sample/lane in the Illumina NextSeq 550.