title: Whole genome sequence of Streptomyces sp.135: a potential new species
design: Genomic DNA of strain 135 was extracted using the QIAGEN Genomic-tip (QIAGEN Biotech Co. LTD) following the manufacturer's instructions. After quality inspection of purity, concentration, and integrity was performed by Nanodrop, Qubit, and 0.35% agarose gel electrophoresis, the genomic DNA library was constructed using a ligation Sequencing Kit (Oxford Nanopore Technologies Ltd)for Nanopore sequencing. The library was sequenced using Promethion sequencer 48. Canu v1.5[18] was used to assemble the filtered subreads. Pilon v1.22[19] was used to further correct the assembly sequence with higher accuracy.