title: GSM3457302: tb09_4414_Elf5_reporter_T2_pos_C3; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were removed and digested with collagenase and hyaluronidase. Tumor cells were sorted with CD45, CD31 and TER119 antibodies to isolate tumor cell enriched population. Based on GFP (Elf5 expression), GFP+ and GFP- cells were further sorted from epithelial enriched population. RNA was harvested using Purelink RNA Mini Kit cat#12183018A and 1 ug of total RNA was used for the construction of sequencing libraries.