title: NextSeq 550 sequencing; RNA-Seq of chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), gibbon (Hylobates lar) and marmoset (Callithrix jacchus) adult testis
design: RNA-Seq of chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), gibbon (Hylobates lar) and marmoset (Callithrix jacchus) adult testis
library_construction_protocol: A piece of < 5 mg was cut on dry-ice from the deep-frozen tissue. RNA was extracted using the RNeasy Micro protocol from QIAGEN. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT). The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).