title: GSM5543277: SRP54 KD 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified using Trizol (Life technologies) and RNeasy Mini kit (Qiagen). Libraries were prepared by the use of Illumina TruSeq Stranded mRNA Sample Preparation kit. Agilent 2100 Bioanalyzer System was used for total RNA and library quality check. Sequencing was conducted on Illumina HiSeq 2500 sequencer.