title: RNA-Seq of zebrafish adult soy diet
design: RNA-Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E. Briefly, each sequencing library was prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments were then PCR-amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology