title: GSM4997090: HL-1 Chromatin Pellet Extract rep2; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were extracted by detergent lysis. Nuclear extracts were prepared by biochemical fractionation. ERCC spike-in standards were added to each nuclear fraction. RNA was extracted with TRIzol reagent. rRNA was depleted with the Illumina Ribo-zero Gold kit. rRNA-depleted RNA was converted to cDNA for sequencing following the manufacturer's instructions for NEBNext Ultra II Directional kit.