title: GSM4774922: Wildtype Gemcitabine 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from snap frozen pancreatic cancer tissues using Direct-zol RNA Miniprep Kit (Cat# R2053, Zymo Research). RNA concentration and purity was measured by NanoDrop (Thermo Scientific), and integrity and quality by Bioanalyzer 2100 (Agilent Technologies) and Qubit (Thermo Fisher Scientific). Indexed, single-index sequencing libraries were prepared from total RNA using the Illumina TruSeq Stranded Total RNA Library Preparation Kit (human/mouse/rat) with rRNA depletion and random-primed cDNA synthesis (Cat# 20020596, Illumina).