title: GSM4775062: GFP-OE-72h_rep1; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq,cells were treated with TRIzol and followed with phase seperation, RNA precipitation and RNA wash. Finally, RNA were dissolved in DEPC water. For RNA-seq, total RNA was subjected to two rounds of purification using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds-cDNA was ligated to adaptors and sequenced with illumina Hiseq platform. For low-input KLF3-GFP positive and negative cells, Samrt-seq2 protocol was applied to construct RNA libraries. RNA-seq and Smart-seq2