title: GSM4876168: RGC that have been transduced with SOX11_WT, replicate2; Mus musculus; RNA-Seq
library_construction_protocol: For viral transduction of E18 RGCs for RNA sequencing, 2.5x10^5 RGC/well were plated on PDL/laminin-coated 6-well plates in quadruplicates. Four hours after plating, 1.5 μl AAV (2x10^13 vector genome (vg)/ml) was added to cultured cells at a MOI of about 10^5 vg/cell. Full medium changes were performed 5.5 and 24 hours after virus exposure. Total RNA was extracted using the RNEasy kit (QIAgen) 72 hours after plating. Library construction and next generation sequencing was performed at Genewiz on 3 RNA samples (500 ng/sample) in duplicates.