title: GSM4773565: reference [ID-005505-Grad_11_Fraction_18]; Pseudomonas aeruginosa PAO1; RNA-Seq
library_construction_protocol: 500 µl gradient fractionation samples were deproteinized with 1% SDS and extracted with an equal volume acidic phenol:chloroform:isoamylalcohol (PCI), and precipitated with ethanol and 1:30 volume NaOAc pH 5.2 with 1 µl glycoblue Samples were solubilized in 50 µl, 5 µl were diluted with 45 µl water, 10 µl were mixed with 10 µl 1:100 diluted ERCC RNA spike-in mix1. RNA samples were prepared as described in Hör et al. 2020 NAR.