title: Whole genome sequencing of Mus musculus Rab27a/b DKO
design: Extracted Tail DNAs were purified using Phenol Chloroform purification method and processed for whole genome sequencing using the Illumina Nextera XT kit following the manufacturers protocol for the library prep.  Sequencing was done on the NextSeq 500 with 150bp paired end sequencing reads as per standard protocols.  Raw sequence FASTQ files were trimmed to remove any primer adapter contamination using Trim Galore! (https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) version 0.6.6 (parameters used: --paired --nextseq 20 trim1).