title: Pseudomonas ogarae F113 Amrz mutant Liquid Stationaryl Phase Replicate 1
design: RNA was extracted from cultures grown in SA medium to exponential (OD600 0.6) and stationary phase (OD600 1.2). Three independent cultures per strain and condition were mixed prior to RNA extraction.  RNA isolation was performed using Trizol (Invitrogen) followed by an isopropanol precipitation. The obtained RNA was treated with DNase (RQ1) and finally was purified using the RNAclean and Concentratortm-5 column (Zymo Research). rRNA depletion, library construction and sequencing was custom performed by  Novogene Co., Ltd. (Beijing, China, and Cambridge, UK) using strand specific Illumina HiSeq paired-end, 2 x 150 bp.