title: GSM4773749: 22mo_Day3Loaded_mouse24; Mus musculus; RNA-Seq
library_construction_protocol: Left and right tibias were stripped of muscle, cut at the distal tibiofibular junction and 2mm distal to the tibial plateau, centrifuged to remove the bone marrow, and snap frozen in liquid nitrogen. Samples were stored at -80°C until RNA extraction. Samples were pulverized using a mikro-dismembrator (Braun Biotech International), and total RNA was extracted using TRIzol (Ambion) with the Total RNA Purification Kit (Norgen #17200). RNA concentrations were measured with the Nanodrop ONE (Thermo Scientific), and RNA quality was evaluated with the Bioanalyzer 2100 (Agilent Technologies). The median RNA integrity number (RIN) was 7.5 with a range of 6.6-8.7. Ribo-Zero Gold