title: Illumina MiSeq sequencing_marine microbial eukaryotes
design: Samples were collected from surface waters (approx. 2L) of marine coastal waters and pre-filtered using 120um sieve.  Pre-filtered samples were then serially filtered through a 3um pore size filter (L) and 0.2um pore size filter (S).  Filters were then sent for Illumina multiplex high throughput sequencing (HTS) to the Integrated Microbiome Resource (IMR) in Dalhousie, Canada using E572F and E1009R forward and reverse primers targeting only the V4 region of the 18S rRNA gene.