title: Nanopore long read sequencing of phodopus sungorous: adult male liver
design: Liver from a Siberian hamster was dissected out and stored at -80. High molecular weight DNA was isolated from 27mg frozen liver using Nanobind Tissue Big DNA Kit from Circulomics. Extracted DNA was needle shared using  a 27G blunt end needle, then size selection was performed by using the Short Read Elimination Kit from Circulomics. Sequencing library was made using the LSK-109 kit, following the Genomic DNA by Ligation Nanopore Protocol. Library was sequenced on one Promethion flow cell (FLO-PRO002).