title: GSM6205778: K562 cells expressing gal4_4, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: To generate RNA-seq libraries of cells expressing each construct, 1 × 106 cells were harvested for each sample 6 d after FACS by centrifugation at 300 × g for 5 min and flash frozen in a dry ice and ethanol bath. RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) and quantified using the Qubit RNA BR Assay Kit (Life Technologies). RNA-seq libraries were prepared by the Whitehead Genome Technology Core facility using the Roche Diagnostics KAPA mRNA HyperPrep Kit.