title: GSM5194329: in vitro heat-killed C. parapsilosis treatment -rep2 [HO_hCp_2]; Homo sapiens; RNA-Seq
library_construction_protocol: In vitro: mRNA was purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocol. RNA quality and quantity were analyzed by Bioanalyzer Instrument (Agilent). In vivo: Mice were sacrificed and tongues were removed. Mouse tongue tissues were removed from the tumor with the help of scalpel. 30mg tumor tissue was homogenized (Bioneer) in RLT buffer and RNA was extracted with RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality and quantity were analyzed by Bioanalyzer Instrument (Agilent). RNA libraries were prepared for sequencing using standard Illumina protocols.