title: GSM3457214: Esg_Pol2-Dam_3; Drosophila melanogaster; OTHER
library_construction_protocol: DNA extraction from dissected midguts and sequencing of methylation-specific PCR amplicons was performed according to the Nature protocols on DamID-Seq published by Vogel et al. (2007) and Marshall et al. (2016). Sequencing libraries were generated according to the Illumina protocol for ChIP-Seq library preparation. Samples were single-end sequenced on an Illumina HiSeq 2000 instrument at 50 bp read length.