title: GSM5510294: Adult Brain, TADA_UAS-Dam-acj6_x_ato sample 1; Drosophila melanogaster; OTHER
library_construction_protocol: Flies were allowed to lay eggs for 2 days at 18˚C in fly food vials. Vials containing those eggs were kept at 18˚C (restrictive temperature) until adult flies eclosed. They were then kept at 18˚C for 3-7 days before being transferred to 29˚C (permissive temperature) for 24 hours. For the Dam-Mef2 experiments in R16A06, a minimum of 90 brains were dissected per replicate, for the Dam-Mef2 experiments in R74G01 cells, 40 brains were dissected, and for the Dam-acj6 in atonal cells, a minimum of 30 brains. 2 biological replicates were performed for each experiment. The DamID protocol is based on (Marshall et al., 2016) with the following changes: After the overnight DpnI digestion , 0.5 µl of DpnI was added for an extra 1 hour incubation and MyTaq™ HS DNA Polymerase was used for the PCR amplification (instead of Advantage 2 cDNA Polymerase). Targeted DamID