title: GSM3456164: 38E748hPDL1: E7 Activated+PDL1 CD8+ T cells, time point 48h; Homo sapiens; RNA-Seq
library_construction_protocol: Tri-Reagent (Sigma-Aldrich) The RNA-seq libraries were prepared using an Illumina TruSeq Stranded Total RNA Sample Preparation kit (Illumina). Library size and quality was assessed in an Agilent DNA 7500 Bioanalyzer assay (Agilent). Each library was sequenced using TruSeq SBS Kit v3-HS, in paired end mode with read length 2x 76bp. On average, we generated 36 million paired-end reads for each sample in a fraction of a sequencing lane on HiSeq2000 (Illumina).