title: GSM4876370: Lsm1_rep1; Saccharomyces cerevisiae S288C; RIP-Seq
library_construction_protocol: After collection, RNA are extracted by hot acid phenol/chloroform method, precipitated with ammonium acetate and ethanol. Samples were treated by RiboZero or Ribominus and libraries were prepared using TruSeq Stranded mRNA kit (Illumina). We strictly followed the protocol except that we started directly at the fragmentation step after the ribozero and we adjusted the number of cycle required to amplified libraries.