title: GSM5511038: IL-10+ B cells rep4; Mus musculus; RNA-Seq
library_construction_protocol: After splenic B cells from IL-10-eGFP Tiger mice were stimulated with 3 µg/ml LPS for 48 h, IL-10+ B cells and IL-10- B cells were sorted and RNA was harvested using Trizol reagent.A total amount of 2 μg RNA per sample was used as input material for the RNA   sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. RNA libraries were prepared for sequencing using standard Illumina protocols