title: GSM4715240: iPSCs - AAVS1_CD326_GATA4_KO_KI_48h (P55 Amplicon-Seq); Homo sapiens; OTHER
library_construction_protocol: Approximately 2 × 10e5 cells were harvested for genomic DNA extraction using 10 μl digestion buffer, which consisted of 100 mM NaCl, 10 mM Tris pH 8, 5 mM EDTA, 0.5% Tween 20 (Sigma), and 1% proteinase K (ABM; 10 mg/ml). Cells released gDNA after running the following program: 56°C for 60 min and 95°C for 10 min. PCR amplicon libraries were prepared for sequencing using standard Illumina protocols PCR Amplicon-Seq