title: GSM5511348: NonTg [SAM24401889]; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspensions were processed using Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 (10X Genomics, PN-1000121) and Chromium Next GEM Chip G Single Cell Kit (10X Genomics, PN-1000120) following manufacturer's user guide. Cell-RT mix was prepared to aim 1,000 cells per sample and applied to Chromium™ Controller for GEM generation and barcoding. Then, samples were subjected to post GEM-RT cleanup, cDNA amplification (11 cycles), and library construction according to the user guide. Sample index PCR was done with 12 cycles. Libraries were then quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Q33230) and profiled by Bioanalyzer High Sensitivity DNA kit (Agilent Technologies 5067-4626). Libraries were sequenced by Novaseq (Illumina) following 10X Genomics sequencing specification. cDNAs and libraries were prepared according to manufacturer instructions (10X Genomics)