title: Illumina HiSeq 2500 sequencing; RNA-seq to investigate the role of FliA-transcribed protein coding genes in Salmonella Typhimurium
design: RNA-seq to investigate the role of FliA-transcribed protein coding genes in Salmonella Typhimurium
library_construction_protocol: Total RNA was extracted from cultures using a Qiagen RNA purification kit according to the manufacturer's protocol.  Four independent biological replicates of strains LT2, TH6827, TH15903 and TH22673 were grown in LBto an OD600 of ~0.5 at 37 °C. Qiagen RNA purification kit according to the manufacturer's protocol.  Illumina TruSeq HT stranded mRNA library preparations(without polyA selection) were prepared at the University of Utah High-Throughput Genomics CoreFacility.