title: GSM5893499: HeLa_shRbm6_2nd_rep; Homo sapiens; RNA-Seq
library_construction_protocol: The phenol-chloroform method was used to purify the total RNA. Genomic DNA contamination was removed by digestion using RQ1 DNase (Promega, Madison, WI, USA). RNA concentrations were determined by measuring the absorbance ratio at 260/280 nm (A260/A280). Electrophoresis through a 1.5% agarose gel was used to assess the RNA integrity. A VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme, Nanjing, China) was used to prepare the RNA-Seq library from 1 μg of purified RNA from each sample. Polyadenylated RNAs from the total RNA were purified, randomly fragmented using heat treatment, and converted into double strand cDNA. Ligation products of 200_x001F_–500 bps were purified and digested using heat-labile uracil-DNA glycosylase (UDG). The cDNA was amplified, purified, and quantified, and then stored at 80 ℃ until sequencing. The Illumina HiSeq X Ten system (Illumina, San Diego, CA, USA) was used to perform RNA-Seq to produce 150 bp paired end sequences.