title: GSM5392525: B1_sgC2_Input; Homo sapiens; ChIP-Seq
library_construction_protocol: In brief, cells were cross-linked directly on the plate using 1% paraformaldehyde for 10 min at room temperature and quenched with glycine. Nuclei were isolated using the Covaris TruCHIP kit and Chromatin extracts were sonicated for 8 min using a Covaris E220 focused ultrasonicator at a peak power of 140, a duty factor of 5 and 200 cycles per burst. Chromatin immunoprecipitation was carried overnight with antibodies bound to dynabeads. Chromatin was de-crosslinked, digested with RNase and Proteinase K and DNA was ourified using a Zymo ChIP DNA kit. KAPA HyperPrep Kit (Kapa Biosystems)