title: GSM5225772: YAP1/AKT1 MS74; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from 30 mg of tissue using Trizol reagent (Invitrogen, Carlsbad, CA) and purified with RNeasy mini kit (Qiagen, Valencia, CA). RNA sample concentration was assessed by Qubit Fluorometric Quantification (Invitrogen, Carlsbad, CA). 1 ug of RNA was poly-A selected, multiplexed RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, San Diego, CA) and sequenced on Illumina HiSeq machine (150nt, paired end). RNA libraries were prepared for sequencing using standard Illumina protocols