title: GSM4875736: NC3_shRNA_RNA-Seq_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from three replicates for cells treated with control shRNA or shRNA against OTUD5, at each stage of neural-conversion, was isolated using Trizol. After confirming that the RNA integration number for each sample was above 8, libraries were prepared using TruSeq Stranded mRNA prep kit with PolyA purificaton. Libraries were sequenced on HiSeq 2500 using a 1x50 single read mode.