title: Illumina MiSeq paired end sequencing; Illumina MiSeq sequencing of the genomic DNA from a single Oikopleura dioica male (ID: I69), polishing reads
library_construction_protocol: 5 ng of DNA were whole-genome amplified to perform sequencing on Illumina MiSeq platform, using the TruePrime WGA Kit (Sygnis, 370025) according to manufacturer's protocol. Magnetic bead purification (Promega, NG2001) was employed for all changes in buffer conditions required for enzymatic reactions and for final buffer suitable for sequencing system. Approximately 1 µg of amplified DNA was sequenced by OIST SQC facility with a 600-cycle MiSeq Reagent Kit v3 (Illumina, MS-102-3003) following the manufacturer's instructions.