title: GSM4876434: PBMC-C2: PBMC ctrl rep2; Mus musculus; RNA-Seq
library_construction_protocol: Blood samples were collected from retro-orbital sinuses of C57BL/6 mice. Blood is collected into a tube containing the anti-coagulant EDTA. Anti-coagulated blood was centrifuged at 180 g at room temperature for 10 min, resulting in platelet-rich plasma. Platelets were isolated from the platelet-rich plasma by centrifugation at room temperature for 10 min at 1,250 g, after which the platelet pellet was lysed in TRIzol Reagent (Invitrogen, ThermoFisher) and frozen at -80 °C for further use. The bottom layer of the first centrifuged blood sample was further used for peripheral blood mononuclear cell (PBMC) isolation via mouse PBMC isolation kit (TBD science, Tianjin) following the manufacturer's instructions. The final PBMC pellet was resuspended and lysed in TRIzol and kept in -80 °C for further use. Total RNAs were extracted according to TRIzol manual instructions. RNA libraries were prepared for sequencing using standard Illumina protocols