title: GSM6205130: PACH22_Null; Homo sapiens; RNA-Seq
library_construction_protocol: 500uL of whole blood pellet was aliquoted for PBMC isolation by negative selection using Stem Cell Technology EasySep Direct Human PBMC isolation kit (catalog #19654). PBMCs were filtered, washed and resuspended. Single cell suspensions were loaded into a Chromium Single-cell Controller (P/N 110211) in accordance with 10X Genomics recommendations for a target cell recovery of 3000 cells per sample. Single-cell RNA libraries were prepared using Chromium™ Single Cell 3' Library & Gel Bead Kit v2 following manufacturer's specifications. Batches 1 and 2 were run on a Flowcell  v2.0 while batches 3 and 4 were run on a Flowcell v2.5.