title: GSM5577918: 301XXM; Mus musculus; RNA-Seq
library_construction_protocol: Hippocampi were dissected from XXF, XXM, XYF, and XYM FCG mice (n=5-6/group), snap frozen in microfuge tubes with liquid nitrogen, and stored at -80ºC prior to DNA isolation. Nucleic acids (DNA/RNA) were isolated from flash-frozen tissues using the All Prep DNA/RNA Mini Kit (Qiagen, Germantown, MD). Illumina's TruSeq Stranded mRNA Library Prep Kit (#20020594, Illumina) was used on 500 ng of total RNA for the preparation of strand-specific sequencing libraries according to manufacturer's guidelines. As previously described, rRNA depletion was performed prior to library construction.