title: GSM4817301: SHR, female, estrus, biological rep 9; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted from whole brain of three alcohol-naïve male rats using the RNeasy Midi Kit with additional cleanup performed using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). Sequencing libraries were constructed using the Illumina TruSeq Stranded RNA Library Prep Kit with Ribo-Zero (Illumina, San Diego, CA, USA), in accordance with the manufacturers protocol. Library quality was assessed using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Samples were sequenced (2x100 paired-end reads) on the Illumina HiSeq2500 (Illumina, San Diego, CA, USA) with 4 libraries multiplexed per lane.