title: GSM4876175: 16S_YoungvAged_Ctrl and ABX [OC1-RL_END]; Mus musculus; OTHER
library_construction_protocol: DNA was extracted using the ZymoBIOMICS DNA Miniprep Kit (Cat# D4300). Libraries were constructed following the the Illumina 16S Metagenomic Sequencing Library Preparation protocol (PN 15044223 Rev. B). Libraries were normalized and multiplexed into a single pool prior to validation by Qubit High-Sensitivity dsDNA, Agilent Bioanalyzer DNA 7500 Chip, and Kapa Illumina Library Quantification qPCR analyses. The final library (8.5pM) and PhiX (0.7pM) were sequenced on the MiSeq System using MiSeq V2 500 cycle kit (250-PE with 8-bp dual indexing).