title: GSM6050553: Mouse, Aca2 R179C induced, 8 weeks, aortic arch; Mus musculus; RNA-Seq
library_construction_protocol: Aortic tissues were cleaned and rinsed with saline to remove residual blood. Tissues were finely minced and digested enzymatically in collagenase/elastase cocktail. Single cell suspensions were then pelleted, washed, and sorted for live cells using fluorescence-activated cell sorting. Individual sample libraries were generated from single cell GEM captures using the Chromium 3' version 3 library prep protocol (10X Genomics). QC was performed following cDNA generation and final library construction.