title: GSM4816920: 2weeks_run; Mus musculus; RNA-Seq
library_construction_protocol: Mashed muscle was enzymatically digested in digestion buffer containing 2 mg/ml Dispase II, 2 mg/ml Collagenase IV, 2 mM CaCl2 and 2% BSA in PBS at 37°C for 10 min, with gentle shaking every 3 min. Viable endothelial cells (CD31+, CD45-, SYTOX blue-) were sorted by a FACS Aria III (BD Bioscience) sorter, and ECs were directly sorted into ice cold collecting medium (EGM2). The collected ECs were placed on ice (no more than 30 min) until loading into 10x genomics chromium. Library construction was performed according to the 10x Chromium Single Cell 3' Reagent Kit v2.