title: GSM5511258: WT1; Mus musculus; RNA-Seq
library_construction_protocol: Spleen was smashed with syringe plungers. Single cells were obtained by filtering smashed spleen with cell strainer. Red blood cells were removed by using red blood cell lysis buffer. The splenocytes were then enriched of CD4+ T cells by MACS sorting followed by FACS sorting of CD4+YFP+ Treg cells.  Total RNA was extracted using a Qiagen RNeasy Mini kit. Illumina TruSeq RNA Sample Prep Kit (Cat#RS-122-2101) was used with a minimum of 100 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.