title: GSM5537253: 3XFlag-DDX18-Flag; Homo sapiens; ChIP-Seq
library_construction_protocol: 3XFlag-DDX18 hESCs were cross-linked with 1% formaldehyde for 10 min at room temperature and quenched with glycine to a final concentration of 0.125 M for another 10 min.Chromatin was sheared by sonication to about 100-500 base pair fragments, cleared by centrifugation, and incubated and IPed  by using FLAG antibody (Sigma,F1804) ChIP DNA libraries were made following Illumina ChIP-Seq Library preparation kit (New England BioLabs).