title: ZTPSN19CW811-244CG
design: DNA extraction used the CTAB/SDS method.  Then V3-V4 of 16 rDNA was amplified by two-step PCR, the libraries were constructed by TruSeq Nano DNALT library Prep Kit,using MiSeq Reagent Kit V3 with Illumina MiSeq platform by PE272.