title: GSM4996499: Human adult eye 1; Homo sapiens; RNA-Seq
library_construction_protocol: Human post-mortem research-consented donor eyes were obtained from the cornea bank at St. Erik Eye Hospital, Stockholm, Sweden. Donors did not present any clinical diagnosis of ocular disease, and samples were anonymized and processed under the general data protection regulation. Two human eyes from the same donor were used (45-year-old male, 32 hours post-mortem). The lens was dissected out and the rest of the retina, except the sclera, was chopped in several small pieces mixed together in 500 uL of digestion buffer (described below). Cells were transported at 4°C to the Eukaryotic Single Cell Genomics Facility (ESCG, SciLifeLab, Stockholm, Sweden) where a 3' cDNA library was prepared for single cell RNA sequencing (scRNA-seq) using the 10X Genomics platform and NovaSeq 6000 software.