title: GSM4875454: LP_HMOPAC2_rep2; Lactiplantibacillus plantarum; RNA-Seq
library_construction_protocol: Cells were suspended in lysis buffer in Lysing Matrix E beadbeating tubes to disrupt cell walls with a FastPrep 56624 bead beater. Bead beating was performed twice at 5.5 m/s for 30 seconds with a 1 min incubation on ice. The Ambion RNAqeous protocol was followed and terminated with total RNA elution into 50 μl of EB solution. Then, total RNA were treated for DNA removal. DNA-free extracts were subjected to ribosomal RNA depletion via the Ribo-Zero Magnetic Kit for bacteria and cleaned up with RNeasy MinElute Cleanup Kit. Purified mRNA was used asseses for quality and used for RNA-seq library preparation. mRNA enriched library preparation was performed with the NEBNext Ultra II Directional kit