title: GSM6205275: heart, cryoinjured; Danio rerio; RNA-Seq
library_construction_protocol: zebrafish with TgBAC(csf1ra:GAL4-VP16)i186; Tg(UAS:NTR-mCherry)c264; Tg(UAS:adra1-3i-T2A-CFP) transgenes subjected to cryoinjury and hearts were collected at 7 dpi and transferred into dissociation solution containing 0.26 U/ml LiberaseTM enzyme mixture (Sigma) and 1X Pluronic F-68 (ThermoFisher) in HBSS. Hearts were incubated in dissociation solution for 30 minutes with shaking at 750 rpm at 37°C with intermittent pipetting (every 5 minutes). Live cells were sorted by FACS. Library was constructed according to the manufacterer's instructions of Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry). (library preparation was done by Genomics technology platform in Max Delbruck Centrum)