title: GSM6205118: D.melanogaster trachea, air control male, rep. 3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA isolation was performed using the NucleoSpin RNA kit (Macherey-Nagel, Dueren, Germany) following manufacturer`s protocol and finally eluted in 40 µl RNase-free H2O. RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer´s recommendations.  All samples were normalized to 2nM and pooled equimolar. The library pool was sequenced on the NextSeq500 (Illumina) with 1x75bp, with 17 to 22 mio reads per sample.