title: GSM6205791: C4-2B_trimmed_sort_dedup_mapped_45M_set 2; Homo sapiens; ChIP-Seq
library_construction_protocol: Disuccinimidyl glutarate (DSG) was used at a final concentration of 2mM and incubated for 45 minutes at room temperature. At the end of fixation, cells were centrifuge at 1500 rpm for 10 minutes. The pellete was washed in PBS for 3 times and the treated with 1% formaldehyde for 10mins at room temperature.  After quenching and cell lysis, chromatin was sonicated using a Bioraptor (Diagenode) ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.