title: GSM5511139: siATF4_SRF_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from the samples above using the miRNeasy Mini Kit (Qiagen, 217004) with on-column DNAse digestion according to the manufacturer's instructions. RNA-sequencing libraries were prepared from total RNA using poly(A) enrichment with the TruSeq stranded mRNA Sample prep from Illumina using 200 ng input RNA. QC was performed with a fragment analyzer using DNF-473-33-SS NGS Fragment 1-6000bp kit. The RNA-sequence libraries were sequenced on a NextSeq 500 using 75 cycles kit High Output (Illumina).