title: Structural and functional determinants inferred from deep mutational scans
design: The unselected CcdB mutant library prepared in Top10Gyr strain was transformed in the sensitive strain (Top10) and RelE reporter strain (Top10Gyr harboring the RelE reporter gene). The mutant libraries were prepared in two biological replicates. These mutant libraries were barcoded and pooled from each condition and further subjected to deep sequencing.