title: GSM3457102: CEBPA.v314vw-INPUT; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed and lysed to extract chromatin. Fixed chromatin was sheared and appropriate antibodies were used to perform the pull down. Aliquotes of the sheared chromatin were used as input. ChIP and INPUT libraries were prepared using the NEB Ultra II kit (E7645S) according to manufacturer's instructions. Briefly, samples were end-prepped, adaptor ligated and cleaned with Agencourt Ampure XP magnetic beads. Adaptor ligated samples were then barcoded during PCR (10 cycles) and cleaned up.