title: GSM5542613: CD19 minigene library DNA-seq 01; Homo sapiens; OTHER
library_construction_protocol: The DNA-seq was performed on the PacBio SMRT sequencing platform at MPI-CBG Dresden. For this purpose, the minigene plasmid library was digested with EcoRI and HindIII (New England Biolabs) and run on an agarose gel. The desired band at the size of 1,301 nt was cut out and purified using QIAquick Gel Extraction Kit (Qiagen). Standard library preparation for run on PacBio SMRT cell.