title: GSM4816778: Polydactyly_03; Homo sapiens; RNA-Seq
library_construction_protocol: Specimens were transferred to freshly prepared dissociation solution composed of 120 μl of Liberase TL (2 mg/ml; Sigma Aldrich) and 840 μl PBS and incubated at 37ºC for 45 min. The cells were collected through 70-μm cell strainer (#352340, Corning) and stored on ice. The tissue was transferred to dissociation solution for second round of dissociation as mentioned above followed by dissociation in Trypsin solution (350 μl PBS, 50 μl 0.25% Trypsin). Cells were washed once and re-suspended in 50-200 μl of freshly prepared PBS-bovine serum albumin (BSA; 1 x PBS and 0.04% BSA). Cell viability was assessed using LUNA-FL dual fluorescence cell counter (Logos biosysteomics). Single cell dissociates were loaded into the Chromium system (10x Genomics) targeting 5,000 cells. The Chromium Single Cell 3' Reagent Kit V2 (10x Genomics) was used to generate scRNA-seq libraries, according to the manufacturer's instructions.Barcoded sequencing libraries were generated using Chromium Single Cell 3′ v2 Reagent Kits and sequenced across by a HiSeq 4000 platform targeting 25,000 reads per cell. Single cell sequencing