title: Illumina MiSeq of Sargasso Sea Water Bacteria
design: eDNA exracted using QIAGEN DNeasy Plant Mini Kit; amplicon libraries prepared following a modified version of the the Illumina 16S metagenomic library prep protocol using primers S-D-Bact-0341-b-S-17/S-D-Bact-0785-a-A-21 (Klindworth et al. 2013); sequencing performed on an Illumina MiSeq (2 x 250 bp)