title: RNA-Seq of T. marneffei: clinical specimen blood of an AIDS patient in Guangzhou, Guangdong, China
design: poly(A)+ RNA was obtained from mycelial and yeast samples using AMPure XP beads (Beckman, CA, USA) and fragmented into short sequences. Fragmentized mRNA was used as template to synthesize the first strand of cDNA with random hexamers (Invitrogen, Shanghai, China). Then dNTPs, RNase and DNA polymerase were used to synthesize the second strand of cDNA. After end-repair and adaptor ligation, cDNA products were amplified using polymerase chain reaction (PCR) and purified to construct a specific cDNA library.