title: scRNA-seq of Homo sapiens expansion cervical epidermis
design: For epidermis isolation, subcutaneous tissues were removed from samples and the epidermis was enzymatically dissociated from the dermis with 2.5mg/ml dispase II (Sigma-Aldrich) incubation at 37 overnight. Epidermal sheets were then manually separated from the dermis and then dissociated into single cells with trypsin-versene (Lonza) incubation at 115 rpm for 10 min at 37 . The generated single-cell suspensions in 50 mL 0.04% bovine serum albumin (BSA, Gibco) in PBS were used for 10x Genomic sequencing.