title: GSM5840713: UCMSC-scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Bulk mRNA was extracted using RNeasy mini kit (QIAGEN). Smart-seq samples were lysed in lysis buffer containing Triton X-100, RNase inhibitor, dNTPs and miliQ H2O preparing for expansion. Single cell mRNA was captured in Nadia platform (Dolomite) using ChemGenes Barcoded beads for Drop-seq (Macosko-2011-10) RNA libraries were prepared for sequencing using standard Illumina protocols