title: rG4-seq 2.0 data of HEK293T cell line
design: RNA was extracted using RNeasy Plus Mini Kit (Qiagen, 74136), subjected to 2 rounds of polyA purification using the Poly(A) PuristMAG Kit (Thermo Scientific, AM1922), and quantified using a Nanospec 1000 (Thermoscientific, USA). Custom library construction was developed for Illumina sequencing (compatible with TruSeq adapters and 6nt index barcoding sequences).