title: ONT
design: 1000 mg of frozen leaf tissue ground with a mortar and pestle. The lysis was carried with Carlson lysis buffer supplemented with -mercaptoethanol. The sample was extracted with chloroform and precipitated with isopropanol. Finally, it was purified using the QIAGEN Blood and Cell Culture DNA Maxi Kit. Size selection was performed using the Circulomics Short Read Eliminator kit to deplete fragments below 10 kb. Then libraries were prepared using the ONT Ligation Sequencing Kit. During sequencing on the PromethION library, re-loads were performed when required. Though yield was slightly lower in sequencing for these samples (over 50 Gb in 24 hours), the read N50 was over 48 kb (up from 28 kb without size selection).