title: Shotgun meta genomics of uncultured domestic animal faces
design: The community DNA sample  extracted from uncultured domestic animal feces (F001) was subjected to shotgun sequencing on the Illumina MiSeq platform at Inqaba Biotechnical Pty Ltd, South Africa. Briefly, DNA samples was fragmented using an enzyme-based approach following the protocol from New England BioLabs Next Ultra II kitTM. Resulting fragments were purified (size selected), end-repaired and an Illumina specific adapter sequence was ligated to all fragments. The sample was quantified, individually indexed followed by a second size selection step using AMPure XP Beads. The libraries was quality controlled on a DNA chip (Agilent 2100 Bioanalyzer) and then sequenced using a MiSeq v3 (600 cycle) kit according to the manufacturers protocol.