title: MiSeq-amplicons of P. falciparum lab strains mixture
design: Malaria positive samples were amplified using gene-specific and indexing  primers targeting 2 polymorphic regions of P. falciparum genes coding for the  circumsporozoite protein (pfcsp), and apical membrane antigen 1 (pfama1). The  sequencing quality and performance were assessed using gDNA extracted from P.  falciparum strains (3D7, 7G8, Dd2 and HB3) mixed at different concentrations  as well as 40 P. falciparum isolates with the complexity of infection (COI)  determined on the basis of the length of pfmsp1 and pfmsp2 amplicon sizes.  Pooled libraries of the above amplicons were sequenced in an Illumina MiSeq  instrument using paired end 2300bp reads and a MiSeq v3 (600 cycles) flow  cell.