title: GSM5582204: Colon Naive ZT16 rep 3; Mus musculus; RNA-Seq
library_construction_protocol: A 5mm section of mid-colon was snap frozen and prepared for RNA Seq.  Tissue was transferred to a Lysing matrix D tube containing Trizol.  Tissue was homogenized using a Bead Mill homogenizer.  RNA was extracted using chloroform then precipitated using isopropanol.  After washing with 75% ethanol, the RNA pellet was re-suspended in RNase free water (50mg/ml). RNA libraries were prepared for sequencing using standard Illumina protocols