title: GSM6052125: dCas9_KAL_tEP3_exp1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was obtained from sorted cells using the ReliaPrep RNA Cell Miniprep System (Promega). RNA quality was evaluated using a NanoDrop 2000 (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer System (Agilent) with an RNA Pico Kit (Agilent, CA) according to the manufacture's instruction. 5 ng of total RNA was used with the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TAKARA, Japan), according to the manufacture's instruction.