title: m6A ip-seq of mouse heart
design: After total RNA was extracted with Trizol reagent, ribosomes were removed and fragmentation reagent was added to fragment the complete mRNA with a length of about 100nt.  The segmented RNA was divided into two parts.  One sample was added with a premixed m6A antibody immunomagnetic bead to enrich mRNA fragments containing m6A methylation.  The other, as a control, directly constructed a conventional transcriptome sequencing library.  M6A antibody immunomagnetic beads were enriched, mRNA fragments with m6A were recovered, and conventional sequencing libraries were constructed according to the library building process of transcriptome.