title: GSM5776161: shCtrl-Normal group 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNAex Pro Reagent (AG, hunan, China) was used to extract total RNA from HK-2 cells according to   the instructions provided by the manufacturer. A total amount of 2 μg isolated RNA per sample was   used for DNA library preparation. Use NEBNext® UltraTM RNA Library Preparation Kit for   Illumina® to generate a sequencing library (Neb, Ipswich, Massachusetts, USA) in accordance with the manufacturer's instructions.