title: GSM5897043: PyMT-Bad3SA 2; Mus musculus; RNA-Seq
library_construction_protocol: PyMT tumor samples snap-frozen in RNAlater® solution (Sigma Aldrich cat#R0901) were thawed on ice. The tissue was retrieved with sterile forceps and excess RNAlater® Solution blotted away with an absorbent lab wipe. Tissue was then promptly lysed with a hand homogenizer (VWR Cat# 47747-370) and RNA extraction was carried out following the manufacturers protocol (QIAGEN's RNAeasy plus mini kit cat#74134). Elution was done using RNase free water. Nanodrop (NanoDrop™ 2000/2000c model) was used to measure the RNA samples concentration and all samples were diluted to 100ng/μl RNA in RNase free water. Samples (20μl each) were packaged in dry ice and sent to Génome Québec for sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols