title: GSM6052140: SHSYRep1Treated_S2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Mini kit (Qiagen Inc.), according to manufacturer's protocol. RNA was quantified using Qubit RNA kit (Invitrogen) and the integrity of RNA was determined in Tape station (Agilent). Strand-specific mRNA sequencing libraries were prepared using Illumina Stranded mRNA Library Prep Kit (Illumina), following the manufacturer's procedure. The fragments were amplified and quantified with KAPA quantification kit (Roche) and the library size was determined using tape station (Agilent). Experiment was performed in three biological replicates for each cell line.