title: TTseq_mES_TIR1_ARID1A_mAID_54h_auxin rep_2
design: RNA was extracted using TRIzol (Invitrogen) following the manufacturers instructions. RNA was treated with DNase using the TURBO DNA-free kit (Ambion) according to the manufacturer but increasing the amount of DNase to 6U and the inactivation reagent to 0.2 volumes. After DNase treatment the beads were removed using QIAshredder columns (QIAGEN). Fragmentation, biotinylation and purification of nascent RNA as in Blumli et al.