title: GSM4819712: CD14_mono_LPS; Homo sapiens; RNA-Seq
library_construction_protocol: Buffy coat from healthy blood donor was diluted by PBS, and PBMC were isolated by Ficol gradient. CD14+ monocytes were isolated from PBMC with anti-CD14 magnetic beads (Miltenyi Biotec). CD14+ monocytes were treated with LPS for 6 h, and were collected and frozen in FBS with 10% DMSO for preservation. For library construction, cells were thawed and resuspened, and the single-cell capturing and downstream library constructions were performed using the Chromium Single Cell 5' library preparation kit according to the manufacturer's protocol (10x Genomics). Full-length cDNA along with cell-barcode identifiers were PCR-amplified and were further prepared as sequencing library.  Instrument BGI MGISEQ2000.