title: GSM5580273: CUTRUN_H3K27me3_PRC1cKO_rep1; Mus musculus; OTHER
library_construction_protocol: CUT&RUN libraries of PRC1ctrl, PRC1cKO, and stella-GFP oocytes were conducted as previously described(30).  ~500 non-growing oocytes isolated from P1 ovaries were pooled as one replicate, and two independent biological replicates were used for CUT&RUN library generation.  The antibodies used were rabbit anti-H2AK119ub1 (1/100; Cell Signaling Technology; 8240), rabbit anti-H3K4me3 (1/100; Cell Signaling Technology; 9751), and rabbit anti-H3K27me3 antibody (1/100; Cell Signaling Technology; 9733).  Libraries were constructed using NEBNext® Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S).  Prepared CUT&RUN libraries were sequenced on the NovaSeq 6000 system (Illumina) with paired-ended 100-bp or 50-bp reads.