title: GSM5896982: Lactobacillus-1; human feces metagenome; OTHER
library_construction_protocol: One gram of fecal sample was weighed and homogenized in 40 mL of PBS with a benchtop homogenizer (MP Biomedical). The sample was then centrifuged at 17,000x g for 5 min and the supernatant was filtered sequentially through a 2 μm and 0.45 μm filter. Phages were then concentrated using a polyethylene glycol (PEG) method(Luong et al., 2020). Briefly, one molar solid NaCl and 10% (v/v) PEG 8000 (Sigma) were dissolved in PBS and incubated overnight at 4°C as recommended for a constant and stable precipitation. The phages mixed in the solution were pelleted by centrifugation at 5,250x g for 1 h at 4°C and re-suspended in 500 μl of SM buffer (NaCl 100 mM, MgSO4.7H2O 8 mM, Tris-Cl 50 mM). Phage DNA was sequenced with the Illumina MiSeq Nano V2 PE_250 bases method using the Nextera XT Kit with an input of 1 ng DNA. All samples were sequenced using paired-end reads Genomic shotgun sequencning