title: GSM4819747: RA_pbmc; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood of RA patient were diluted by PBS, and PBMC were isolated by Ficol gradient. PBMC were frozen in FBS with 10% DMSO for preservation. For library construction, cells were thawed and resuspened, and the single-cell capturing and downstream library constructions were performed using the Chromium Single Cell 5' library preparation kit according to the manufacturer's protocol (10x Genomics). Full-length cDNA along with cell-barcode identifiers were PCR-amplified and were further prepared as sequencing library.  Instrument BGI MGISEQ2000.