title: GSM5512843: HiC.07_CTCFWAPL_24h; Mus musculus; Hi-C
library_construction_protocol: Hi-C data were generated using as a previous published protocol (Rao et al., 2014) with minor modifications (Haarhuis et al., 2017). For each template, 10 million cells were harvested and crosslinked using 2% formaldehyde. Crosslinked DNA was digested in nucleus using MboI, and biotinylated nucleotides were incorporated at the restriction overhangs and joined by blunt-end ligation. The ligated DNA was enriched in a streptavidin pull-down. Hi-C libraries were prepared using a standard end-repair and A-tailing method.