title: Cut and Run of PrePregnancy Mammary Epithelial Cell Derived Organoids with Essential Media Raw Sequences
design: MEC derived pre and post-pregnancy organoids (n= 2-3 wells per technical replicate) permeabilized with digitonin, following incubation with antibody against H3K27ac histone marks 12 hours. Antibody-chromatin complexes were fragmented with pA-MNAse and purified utilizing Phenol-Chloroform. Amplification and barcoding using Clontech DNA Smart ChIP-Seq kit in accordance with the manufacturers instructions, then pooled for sequencing on an Illumina NextSeq500, 76bp paired-end run.