title: Shotgun sequencing Ambystoma tigrinum DNA, second round
design: * The shotgun genomic libraries were prepared with the Hyper Library construction kit from Kapa Biosystems (Roche). * The libraries were quantitated by qPCR and sequenced on one lane for 151 cycles from each end of the fragments on a NovaSeq 6000  using a NovaSeq S4 reagent kit. Fastq files were generated and demultiplexed with with the bcl2fastq v2.20 Conversion Software (Illumina).