title: GSM5195452: WT_H3K27ac (ChIP-seq); Mus musculus; ChIP-Seq
library_construction_protocol: Freshly  cerebellar tissues isolated from of WT and EED-/-mice. Fixed ,Lysates,sonicated ,incubated with antibodies,cross-linking . DNA was extracted and resuspended in nuclease-free water.DNA libraries generated from ChIP-DNA and input-DNA were deep-sequenced using HiSeq single-end 50bp. ChIP's DNA was subjected to end-repair and then was 3'adenylated. Adaptors were ligated to the ends of these 3'adenylated fragments. Next, polymerase chain reaction amplification was to amplify those fragments with adaptors from previous step.