title: WGBS of mus musculus: adult male sperm
design: The sequencing libraries of 18 sperm DNA samples were constructed using NEBNext Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturers instructions. In brief, DNA were fragmented into 100300 bp sections by sonication with a Diagenome sonicator. Then, they were subjected to end-repair, A-tailing and adaptor ligation. After size selection (200-300 bp) of the adaptor-ligated DNAs by 2% gel electrophoresis, bisulfite conversion was performed using the EZ DNA Methylation Gold kit (Zymo Research, Irvine, CA, USA) according to the manufacturers instructions. Bisulfite treated DNA was then PCR amplified using ZymoTaq DNA Polymerase with 14-15 cycles. Bisulfite-converted libraries were sequenced with 2 150 bp paired-end (PE150) reads on an Illumina HiSeq 4014 platform. The spiked-in lambda DNA was used to determine the bisulfite conversion efficiency.