title: RNA-seq small RNA of homo sapiens :liver tissue
design: Total RNA was purified with RNA Easy Mini Kit (QIAGEN) and libraries construction was done using the Nextflex Small RNA-Seq kit v3 (Bio Scientific, PerkinElmer, USA). The 3 and 5 modified adapters were ligated specifically onto small RNAs and amplified for 18 cycles of PCR after reverse transcription. Barcodes were also introduced during the PCR steps. After size selection, the libraries were pooled and loaded onto a MiSeq (Illumina) sequencer, using the MiSeq Reagent Kit v3 150-cycle (Illumina).