title: hsp70 expression on round goby Neogobius melanostomus
design: ABI 3730 DNA analyzer for capillary Sanger-sequencing with: 96 C for 1 min, 96 C for 30 sec, 59 or 61 C, depending the primer for 15 sec, 60 C for 4 min, for 30 cycles and then at 72 C for 10 min.