title: GSM5394000: Tri-PyMT_invitro_Mix; Mus musculus; RNA-Seq
library_construction_protocol: Tri-PyMT cells were cultured in growth medium with 10% FBS. RFP+, GFP+ and Double+ cells were sorted by flow cytometry and re-mixed with same numbers. 10x Genomics Chromium was employed to add barcoded primer gel beads to each single cell.  The captured polyA mRNA from single cells was reversely transcribed, barcoded and amplified following standard protocol of10X Genomics Chromium Single-cell 3' Library RNA-Seq Assays , followed by sequencing in Illumina NovaSeq6000 at the Genomics and Epigenetics Core Facility at Weill Cornell Medicine.