title: Nanopore of Phodopus roborovskii: adult female
design: To generate Oxford Nanopore long reads, approximately 15 g of genomic DNA was processed according to the Ligation Sequencing Kit 1D (SQK-LSK109) protocol. Briefly, DNA fragments were repaired using NEBNext FFPE DNA Repair Mix (New England Biolabs). After end-repair and 3'-adenylation with NEBNext End repair/dA-tailing Module reagents (New England Biolabs), the Oxford Nanopore sequencing adapters were ligated using the NEBNext Quick Ligation Module (E6056) (New England Biolabs). The final library was sequenced using a PromethION DNA sequencer (Oxford Nanopore, Oxford, UK). Guppy software (https://community.nanopore-tech.com/posts/guppy-v4-0-11-release) was used to conduct base calling of the raw signal data. These obtained data were then filtered to remove reads with adapter sequences.