title: Illumina NovaSeq 6000 sequencing; Transcriptomes of novel human genomic safe harbor sites for gene and cell therapies
design: Transcriptomes of novel human genomic safe harbor sites for gene and cell therapies
library_construction_protocol: Cell lines were harvested following transfection into safe harbor loci. Single-cell from human PBMCs were isolated following transfection and processed using 10x genomics.    Jurkat and HEK293T cells were edited to integrate CMV-mRuby expressing cassette into Rogi2 genomic safe harbor site using Cas9 RNP RNA for bulk RNA-seq was extracted using Trizol. Single-cell RNA sequencing was performed in accordance to 10x genomics involving microfluidic capture (cat number 1000075). Briefly, single cells were co- encapsulated with gel beads (10X Genomics, Single cell 10X libraries were constructed from the isolated single cells following the Chromium Single Cell 3สน GEM, Library & Gel Bead Kit v3 (10X Genomics, PN-1000075). Briefly, single cells were co- encapsulated with gel beads (10X Genomics, 2000059) in droplets using Chromium Single Cell B Chip (10X Genomics, 1000074)