title: GSM6102463: naive CD4+ T cells, Mettl3 WT3, 48H; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol Reagent (Invitrogen) following standard protocols and subjected to RNA-Seq performed by Novogene. Paired-end RNA-Seq was performed to yield more than 100 million reads per sample, which allowed us to obtain not only transcript expression but also alternative PAS usage.Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. RNA libraries were prepared for sequencing using standard Illumina protocols