title: WGS Streptomyces_sp_09MW18-IS
design: For whole-genome shotgun sequencing, the genomic DNA was extracted using the UltraClean microbial DNA isolation kit (Mo Bio Laboratories, Inc.) and sequenced using nanopore GridIon. The DNA was concentrated using Thermo Fisher Vacufuge (Thermo Fisher Scientific, Waltham, MA). The library was prepared using the Nanopore Rapid Sequencing (SQK-RAD004) kit (Oxford Nanopore Technologies, Inc., Oxford, UK) using the protocol supplied by the manufacturer. DNA was eluted using EB Buffer after bead purification. The library was quality checked using an Invitrogen Qubit 4 Fluorometer and 1X Qubit dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific, Waltham, MA). Adaptor was ligated and loaded on the flowcell via the SpotON sample port according to the manufacturer protocol.