title: Whole Genome sequence reads of Pythium myriotylum
design: The whole genome sequencing libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina.DNA was enzymatically fragmented by targeting 200bp to 300bp fragments size using a Fragmentation reagent and the DNA fragments undergo end repair where in the mix converts the overhangs resulting from fragmentation into blunt ends. The 3 to 5 exonuclease activity of end repair mix removes the 3 overhangs and polymerase activity fills in the 5 overhangs. To the blunt ended fragments adenylation was performed by adding single A nucleotide to the 3 ends. To the adenylated fragments loop adapters were ligated and cleaved with uracil-specific excision reagent (USER) enzyme. The samples were purified using 0.9X AMPure XP beads (Catalog: A63881, Beckman Coulter) and the DNA was then amplified by 10 cycles of PCR using NEBNext Ultra II Q5 master mix, and to facilitate multiplexing, NEBNext Multiplex Oligos for Illumina kit was used . The amplified products were purified using 0.9X AMPure XP beads (Catalog: A63881, Beckman Coulter) and the final DNA library was eluted in 15 uLs of 0.1X TE buffer.The DNA library was denatured using freshly prepared NaoHand applied onto Illumina Hiseq system for 150 nucleotide pair-end sequencing  using HiSeq X reagent kit (Catalog: FC-501-2501) and the data was extracted to FastQ format using the Casava software from Illumina