title: FAIRE-seq of T. cruzi: Epimastigote
design: The FAIRE samples were prepared as described in (SIMON et al., 2012) with some modifications. 2 x 107 parasites (in biological duplicates) were fixed for 5 minutes with formaldehyde, directly added to the medium to a final concentration of 1%. Formaldehyde was quenched with 125 mM glycine, followed by extensive washing with PBS. Pellets were resuspended in 1 mL of cold lysis buffer TELT (50 mM Tris-HCl pH 8.0, 62.5 mM EDTA, 2.5 M LiCl, 4% Triton X-100) and sonicated with six cycles of 30 seconds with an interval of 1 minute each (Output 4 and Duty 30 parameters) at Tommy Ultrasonic Disruptor UD-201 apparatus.