title: GSM5840550: CTCF_16HD_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Briefly, approximately 10 million cells were cross-linked with 1% formaldehyde in PBS at room temperature for 10 min and neutralized with 0.125M glycine. After sonication, ~50µg soluble chromatin was incubated with 1-5μg of antibody at 40C overnight. Immunoprecipitated complexes were collected using Dynabeads A/G (Invitrogen). Subsequently, immuno-complexes were washed, DNA extracted, and purified by QIAquick Spin columns (Qiagen). For ChIP-seq, the extracted DNA was ligated to Illumina UDI adaptors followed by deep sequencing with Illumina's HiSeq 4000 according to the manufacturer's instructions. ChIP enriched DNA was purified, and 10 ng used to prepare sequencing libraries with the KAPA HTP/LTP Library Preparation Kits