title: microbiome of Cochliomyia hominivoax: mass-reared adult male body
design: The 16SV4 region of the 16S rRNA gene was amplified using 16S V4: 515F-806R primers (Walters 2016), with attached barcode. Sequencing libraries were generated using NEBNext  Ultra DNA Library Pre Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA) following manufacturer's recommendations with index codes added. Paired-end reads were assigned to samples based on their unique barcode and then truncated by cutting off the barcode and primer sequence. Quality filtering on the raw tags was performed under specific filtering conditions to obtain the high-quality clean tags.