title: tomato WGBS root -Pi replicate 3
design: Genomic DNA (gDNA) was extracted from plant tissues using the DNeasy Plant minikit (Qiagen, the Netherlands). A total amount of 100 ng genomic DNA spiked with 0.5 ng lambda DNA were fragmented by sonication to 200-300 bp with Covaris S220. These DNA fragments were treated with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research, USA), and the library was constructed and sequenced with 150 bp pair-end sequencing on Illumina Novaseq platform by Novogene (China).