title: RABV India
design: Viral RNA for sequencing was isolated from dog brain samples using Zymogen Research Kit (R1054) according to the manufacturersprotocol, concentration was measured using Qubit (Invitrogen). cDNAwas prepared using LunaScript kit (E3010L). Multiplex tiled PCR (Q5 Hot Start Polymersase Enzyme) was carried out using custom designedoverlapping primers to amplify 400 bp regions across the genome andwith 90-100 bp overlapping segments.