title: RNA-Seq of tissue-specific DAF-50 degradation worms: Hypodermis cells of intestnal DAF-2 degradation replicate 3
design: RNAs were amplified with oligo-dT, then reverse transcribed to cDNA based on polyA tail. The template was switched to the 5' end of the RNA and the full-length cDNA was generated by PCR. Purified cDNA was fragmented into small pieces with fragment buffer by PCR, and the product was purified and selected by the Agencourt AMPure XP-Medium kit. cDNA was quantified by Agilent Technologies 2127 bioanalyzer. The double stranded PCR product undergo QC step was heat denatured and circularized by the splint oligo sequence. The single strand circle DNA was formatted as the final library.