title: 19s rRNA Sequencing of Qingfei Paidu Decoction in Pneumonia Model Mice
design: Fecal DNA was isolated using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Dusseldorf, Germany). Illumina sequencing was done based on published methods. The V3V4 region of the 16S ribosomal RNA gene was amplified and sequenced. Sequence reads were analyzed using QIIME software 1.9.1. Functional profiles of microbial communities for 16S rRNA sequencing were predicted using PICRUSt5 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States)