title: Metagenomic RNA sequencing of bat guano
design: SuperScript IV (SSIV) Reverse Transcriptase (RT) kit was used for first strand synthesis at the following temperatures: 23 C for ten minutes, 50 C for 15 minutes, 10C for five minutes, and four C for two minutes. The second strand of cDNA was synthesized with Invitrogen Sequenase Polymerase kit at the following temperatures: 10C for 2 minutes, ramping up to 37C over 8 minutes, and 37C for 8 minutes. Libraries of each cDNA sample were prepared with Nextera XT (Illumina) kit following the recommended protocol with amplification of 16 cycles