title: GSM5291846: PBS1; Mus musculus; RNA-Seq
library_construction_protocol: Microglia are isolated from mouse brain following protocol described in Hickman et al. 2013. Total RNA was isolated using RNeasy Plus Micro kit (Qiagen) using the total RNA isolation protocol. Total RNA was isolated and sequencing libraries were made using QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) . Sequencing was done using an Illumina MiniSeq