title: RNA of Phodopus roborovskii: adult female, heart, kidney, liver, lung, muscle, spleen
design: The individual used for the genomic sequencing was also used for the transcriptome sequencing, providing necessary gene expression data for the genome sequence annotation. That six tissues of our four individuals, including heart, kidney, liver, lung, muscle, and spleen were collected for the following transcriptome sequencing. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and disrupted into short segments. This was followed by cDNA synthesis using random hexamer primer and reverse transcriptase. After end-repair, adapter-ligation, and PCR amplification, each paired-end cDNA library was sequenced with a read length of 150 bp using the Illumina NovaSeq 6000 sequencing platform.