title: B-1
design: The total genomic DNA from strain YIM 93972 was extracted using the DNeasy Power Soil Kit (QIAGEN, Hilden, Germany). The quality of DNA was assessed by 1% agarose gel electrophoresis, and the purity were measured using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and Qubit2.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Qualified genomic DNA (5 g) was sheared with a Covaris g-TUBE shearing device (Covaris, Woburn, MA, USA) (8-12 kb insert sizes). The fragmented DNA was purified by AMPure PB beads (Pacific Biosciences, Menlo Park, CA, USA). The SMRTbell DNA template libraries (with a fragment size >10kb) were constructed using the DNA Template Prep Kit 4.0 (Pacific Biosciences, Menlo Park, CA, USA). SMRT sequencing was performed applying a Pacific Biosciences RSII sequencer (Pacific Biosciences, Menlo Park, CA, USA). Genome assembling and annotation Hierarchical Genome Assembly Process (HGAP) pipeline was used to a generate high quality de novo assembly of the genome with default parameters.The mapping results were processed with SAM tools, and raw SNPs/Indels were called using the pileup command of the SAM tools package. The rRNAs and tRNAs of YIM 93972 were predicted using RNAmmer and tRNAscan-SE, respectively. The circular maps were generated by DNAPlotter.