title: NextSeq 550 sequencing; RNA-Seq of FACS-sorted, KIR-CD39-,KIR+CD39- and KIR+CD39+, Uterine NK cells (uNK cells) from human decidual samples
design: RNA-Seq of FACS-sorted, KIR-CD39-,KIR+CD39- and KIR+CD39+, Uterine NK cells (uNK cells) from human decidual samples
library_construction_protocol: KIR-CD39-, KIR+CD39-, and KIR+CD39+ uNK cell subsets were sorted after pre-gating on alive CD45+CD3-CD56+CD16-CD57-NKG2A+ cells directly into 300 uL RLT buffer (Qiagen) in a sterile RNAse DNAse free 1.5 mL Eppendorf tube and frozen on dry ice immediately after sorting. RNA was isolated with the RNeasy Plus Micro Kit (Qiagen) and quality tested with the Bioanalyzer Pico kit (Agilent).  cDNA was generated with the Smart-seq v4 (Clontech)