title: GSM5620586: Tumor in Keytruda-treated group (Visium); Mus musculus; RNA-Seq
library_construction_protocol: Tumor were prepared according to Visium spatial protocols of tissue preparation guide (10x genomics). Firstly, freshly obtained tissue samples were snap frozen in liquid nitrogen, then embedded in chilled Optimal Cutting Temperature compound (OCT; Tissue-Tek) and frozen on dry ice, then stored at -80°C in a sealed container for later use. For Visium samples preparation, OCT-embedded tissue blocks were cut to 10 um thick using a LEICA CM1950 machine and mounted on the Visium spatial gene expression slide. 10x genomics visium spatial gene expression pipeline