title: eccDNA mus musculus after mtDNA removal
design: About 500 ng of Phi29 amplified DNA Products was sheared by sonication (Covaris LE220) to generate a median size of 400 bp. Then, 30 ng of fragmented DNA was used as input for library construction using MGIEasy DNA Library Preparation Kit (MGI-BGI, China). Size distribution and quality of each library were examined by the Bioanalyzer 2100 (Agilent). DNA libraries were sequenced (PE150) on the MGISeq-2000 platform (BGI-Qingdao, Qingdao, China).