title: Paired end WT RNA-Seq replicate1
design: rRNA was removed from the total RNA using a NEBNext rRNA Depletion Kit. Thereafter, the remaining mRNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into the first-strand complementary DNA (cDNA) using SuperScript II reverse transcriptase and random primers. This is followed by second-strand cDNA synthesis using DNA Polymerase I, RNase H, and deoxy-uridine triphosphate. cDNA fragments then go through an end repair process, the addition of a single A base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library