title: GSM6098600: HeLa, sgG2E3, H3K9me3, rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 15 minutes at room temperature following a final concentration of 125 mM and incubated for 5 min to stop crosslinking. After cell lysis, chromatin was sonicated.Subsequent ChIP assays for H3K4me3, H3K9me3 and H3K14Ub were performed essentially as described (Li et al., 2020). . DNA degradation and contamination was monitored on 1% agarose gels. DNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). DNA concentration was measured using Qubit® DNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). A total amount of 50ｎg DNA per sample was used as input material for the Chip sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina®(NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.