title: RNAseq analysis of potato leafhoppers collected in Iowa in 2021
design: Potato leafhoppers were captured in 2020 and 2021 by the Midwest Suction Trap Network, pooled by state. Total RNA was extracted using the RNeasy Mini Kit and depleted of ribosomal RNA with the Illumina Ribo-Zero Plus rRNA Depletion Kit. Sequencing libraries were prepared using the Illumina ScriptSeq RNA-Seq Library Preparation Kit and sequenced on an Illumina NovaSeq 6000 at the University of Illinois. Reads from each library were assembled de novo with Trinity. Assembled contigs were compared to a customized database that included predicted reference invertebrate and virus amino acid sequences using USEARCH. Contigs with significant similarity to virus sequences were compared to the NCBI nonredundant protein database using BLASTX.