title: Phoma segeticola var. camelliae mock 1
design: After cultured on PDA for 6 d, 4 mycelial plugs (6 mm diameter) from the edge of the strain GZSQ-4 colony were transferred to PDB and inoculated in shaker with 25C, 180 rpm for 67 h. Mycelia did not treat with ZSM set as the control treatment (Mock treatment)for 3 h.Five biological replicates were conducted.Finally, the mycelia were filtered with qualitative filter paper and washed with ddH2O three times to obtain clean strain GZSQ-4 mycelia. Clean GZSQ-4 mycelia were ground into fine powder under the protection of liquid nitrogen. About 100 mg mycelia powder were used for RNA extraction. Total RNAs were extracted using the EASYspin Plus Plant RNA Kit (Aidlab, China). A total amount of 3 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample.