title: GSM6051616: F3_nonnaive_rep2; Daphnia magna; Bisulfite-Seq
library_construction_protocol: DNA was extracted from frozen tissues at -80 °C by using the MasterPure Complete DNA and RNA Purification Kit (Epicenter, USA), according to the instructions of the manufacturer. DNA was fragmented with the restriction enzyme MspI (New England BioLabs, USA) and purified using the GeneJET PCR purfication kit (Thermo Fisher Scientific, USA). After this, library preparation was achieved with the NEBNext Ultra II DNA library prep kit (New England Biolabs, USA) by using methylated adapters for Illumina sequencing.