title: RNA-Seq of Young Thrombocytes
design: RNA from sorted RFP+ thrombocytes were prepared according to 10x Genomics protocol, and sequencing was performed after successful library preparation and quality control. Briefly, the sorted cells were loaded immediately onto 10x Genomics Chromium Chip B. The barcoded cDNA libraries were generated using 10x Genomics Chromium Single Cell 3 GEM library and Gel Bead Kits V3 according to the manufacturers instructions. The library sequencing on Illumina Hiseq 2500 V3 was performed on a depth of a minimum of 20,000 read pairs/cell for each library, and Cell Ranger software (3.0.0) version was used for barcode recovery