title: PacBio sequencing of Lactobacillus brevis Oregon-R-modENCODE strain BDGP6
design: gDNA was isolated and sent to the National Center for Genome Resources (NCGR), Santa Fe, New Mexico for whole-genome sequencing using Pacific Biosciences (PacBio, Menlo Park, CA) long read sequencing on the RS II instrument. A single molecule real-time (SMRT) cell library was constructed with 5-10 g DNA using the PacBio 20-kbps protocol and sequenced on one SMRT cell using P6 polymerase and C4 chemistry with 6hr movie times