title: WGS of human:PDAC
design: Frozen tumor tissues were aseptically sliced into appropriate size and metagenomic DNA  extraction was carried out using  Pathogen Lysis Tubes (QIAGEN) and  AllPrep DNA/RNA Mini Kit (QIAGEN). DNA samples were applied to library construction and whole-genome sequencing on the Illumina platform and yielded 2x150 bp paired-end reads. The human DNA sequences were removed by alignment on reference genome and reads for microbiome were obtained for subsequent analyses.