title: N. vectensis genomic long reads (Stowers)
design: N. vectensis high molecular weight DNA was extracted at Dovetail Genomics. Samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). The PacBio SMRTbell library (~20kb) for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol. The pooled library was bound to polymerase using the Sequel Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel using the MagBead Kit V2 (PacBio). Sequencing was performed on the PacBio Sequel SMRT cell, using Instrument Control Software Version 5.0.0.6235, Primary analysis software Version 5.0.0.6236 and SMRT Link Version 5.0.0.6792.