title: RNA-seq of Dreissena polymorpha exposed: adult digestive gland
design: Digestive glands were dissected, snap frozen in liquid nitrogen and stored at -80C. Total RNA extraction was done using RNeasy MinElute kit (Qiagen) according to the manufacturers protocol with a slight modification. Briefly, tissus disruption was done first with a pestle and then with a beads beater with glass bead (200 nm) for 30 sec at max speed. Genomic DNA was removed by DNase I digestion on column and total RNA was eluted in ultra pure water. Total RNA was quantified with a nanodrop and RNA integrity was assessed on a bioanalyzeur (Agilent). Total RNA samples were stored at -80C.