title: CT_F1_RNA-Seq of Caenorhabditis elegans: Young Adult
design: mRNA profiles of young adult wild-type (WT) fed with or without HFD and their recovered progeny were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000.Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the C. elegans genome. Altered expression of 20 genes was confirmed with qRTPCR, demonstrating the high degree of sensitivity of the RNA-seq method. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within C. elegans.