title: RNA-Seq of Rimicaris exoculata
design: Stringent quality checking and data cleaning were performed by filter_fq software. After sequencing, adapters, unknown nucleotides larger than 5% and low quality reads (the reads which quality value <= 10 is more than 20% of the total) were discarded. Based on the clean reads, assembly was carried out with Trinity [20-22] as described for de novo transcriptome assembly without a reference genome, by set --seqType fq --min_contig_length 100 --min_glue 5 --group_pairs_distance 250 --path_reinforcement_distance 90 --min_kmer_cov 5, to acquire non-redundant Unigenes as long as possible.