title: Whole exome sequencing of colorectal cancer cousin pairs.: Sample 10281X1_443_3
design: DNA samples (1 ug) were sheared using a Covaris S2 Focused-ultrasonicator and libraries were prepared using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit (cat# FC-121-3001 and FC-121-3002) with an average insert size of 350 bp.  Adapter-ligated molecules were analyzed by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824) and the molarity of individual libraries were normalized to 10 nM in preparation for Illumina sequence analysis.