title: RNA-Seq of Cynopterus: unknown ethanol-preserved, frozen tissue
design: We sequenced a subset of cDNA libraries to evaluate the composition of extracted products. Library preparation was performed with a KAPA Biosystems LTP Library Preparation kit and with UGA iTru style dual indices, using 30 cycles for amplification. Following amplification, a 1  SPRI purification was performed to remove primer and adapter dimer. Samples were pooled into a single run of the Illumina MiSeq 2  150 PE v2. Due to the insert length, the run was limited to 75 cycles.