title: metagenome of mouse gut microbiome
design: The universal primer 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with 12 unique barcodes to each sample were used to amplify the V4-V5 hypervariable region of 16S rRNA gene for pyrosequencing using Miseq sequencer