title: GSM6052310: ELAC2A537T TRAMP +/- 10weeks rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Individual prostate lobes were dissected as described previously by Zingiryan et al., 2017. Briefly, an OPMI 6-SFC Universal Microscope (Zeiss) was used to remove each prostate lobe to isolate high quality and pure tissue. Upon removal of the urogenital system from the sacrificed animal, all tissue was completely submerged in PBS. The seminal vesicles, bladder, urethra and surrounding connective or adipose tissue were removed and then the individual ventral, lateral, anterior and dorsal lobes were dissected and frozen in liquid nitrogen. RNA was isolated from the ventral and lateral prostate lobes as recommended by Zingiryan et al., 2017, using the miRNeasy Mini kit (Qiagen) incorporating an on-column RNase-free DNase digestion to remove all DNA. RNA sequencing was performed on total prostate RNA from three of each control (WT or TRAMP) and the four mutant mouse lines by the Australian Genomic Research Facility (Melbourne, Australia). Sequencing was performed using the Illumina NovaSeq platform, according to the Illumina TruSeq RNA-Seq protocol and as we have done previously (Rudler et al., 2019).