title: GSM5840488: KFNV_ATAC_rep2; Mus musculus; ATAC-seq
library_construction_protocol: A total of 50,000 cells were collected at day 4 of reprogramming in replicates. Cells were washed once with 50 μL of cold PBS and trypsinized, centrifuged at 300 x g for 5 min and   Nuclei were extracted and QC was performed. After the QC, the nuclei pellets were resuspended in the Tn5 transposase reaction mix consisting of transposase and equimolar adapter1 and adapter 2. The mix of transposition reaction was incubated at 37°C for 30 min. PCR was then performed to enrich the library. The final libraries were purified with the AMPure beads and library quality was assessed. Qubit 2.0 was used for quantification of the final library. The final library was diluted to 1ng/µl based on qubit value. The insert size of the library fragment was assessed with NGS3K. Effective concentration of library was determined by Q-PCR. Finally, the qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.