title: GSM5004236: P.mex/lat #12_Brain; Poecilia mexicana x Poecilia latipinna; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol Reagent (Thermo Fisher Scientific, Waltham, USA) according to the supplier's recommendation. All samples were treated with DNase. Total RNA concentration was determined using a Qubit 2.0 fluorometer (Life Technologies, Grand Island, NY, USA). RNA quality was verified on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8.0 prior to sequencing. Library construction follows Illumina TruSeq mRNA sequencing library preparation protocol