title: 18S rRNA gene samples collected in Lubuge in March
design: Water DNA was extracted using a MicroElute Genomic DNA kit (Omega, USA) according to the manufacturers instructions. The total DNA was eluted in 50 l of elution buffer using a modified procedure described by the manufacturer. The bacterial 16S rRNA gene and the V9 region of the eukaryotic 18S rRNA gene were ampli-fied using Bakt-805R and Bakt_341f primers, and 1380F and 1510R primers by PCR according to previously described methods.