title: ChIP-Seq of D. melanogaster: S2 Cells
design: Chemically cross-linked S2 cells were lysed and sonicated to shear DNA to 300-500 bp fragments. Lysate was reverse-crosslinked and treated with RNAse A and Proteinase K prior to DNA extraction. DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB# E7645), using Ampure XP beads for cleanup, without size selection.