title: Illumina whole genome sequencing of Helicoverpa zea: male pupa
design: DNA extraction was performed from a single male (homogametic, ZZ sex chromosome) whole insect (ilHelZeax1) using the MagAttract HMW DNA Kit modified from manufacturer instructions to include gentle inversion to mix all components, an additional wash step, and eluting DNA from beads with three separate additions of 115.0 l AE buffer at 37C. DNA was prepared for Illumina sequencing with the TruSeq DNA PCR-Free Low Throughput Library Prep Kit with TruSeq DNA UD Indexes using standard protocols for a library with a 350bp insert size and 50bp standard deviation. Sequencing was performed on an Illumina NovaSeq 6000 system.