title: GSM5852277: M2; Homo sapiens; RNA-Seq
library_construction_protocol: Single cells were isolated from patient-derived breast using Tumor Dissociation Kit protocol (Miltenyi Biotec, 130-095-929).  Single cells  were processed using the Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1 and Chromium Next GEM Chip G Single Cell Kit according to the manufacturer's instructions in the Chromium Next GEM Single Cell 3′ Reagents Kits V3.1 User Guide(﻿10X Genomics). Briefly, 8,000 cells were loaded into each chip channel and partitioned into Gel Beads-in-emulsion (GEMs) on the Chromium Controller, followed by cell lysis and barcoded RNA reverse transcription.﻿ Library preparation was performed after breaking of single-cell emulsions, and included cDNA amplification, fragmentation, hearing and 5′ adaptor and sample index attachment. Sequencing was performed on a HiSeq X (Illumina, Inc., San Diego, CA)