title: MiSeq of soil fungi: temperate agroforestry
design: Primer pair fITS7/ITS4; Thermocycling conditions were as follows: initial denaturation (95C for 2 min), 3 touch-up cycles of denaturation (95C for 20 sec), annealing (50C for 30 sec), and elongation (68C for 30 sec), 30 cycles of denaturation (95C for 20 sec), annealing (58C for 30 sec), and elongation (68C for 30 sec), and final elongation (68C for 5 min). Libraries were normalized using photo densitometry.