title: GSM5348675: RNApolII IP in sgControl; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were stored in Trizol and RNA extracted using the Zymogenetics directZol miniprep kit. For ChIp Seq, Cells were crosslinked in formaldehyde and ChIP was performed. RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep v2 kit. Fixed ChIP samples were processed by micrococcal nuclease treatment, followed by construction of single strand libraries.