title: Short-read metagenomic sequencing of human HCT patient stool sample
design: Samples were subjected to beadbeating prior to extraction using the Mini-Beadbeater-16 (Biospec Products). DNA was extracted from stool per manufacturer instructions (QIAamp DNA Stool Mini Kit, Qiagen). The Nextera XT DNA Library Prep Kit (Illumina) was used on DNA extractions to create sequencing libraries. When library preparations were unsuccessful using this method, we instead used the Nextera DNA Flex Library Prep Kit (Illumina).