title: Given WT  donor T cells and treated  Dexamathosone (5mg/kg) at days 3,10, 15 and 20 after bone marrow  transplantation
design: Microbial DNAs from  1-3 mg of mouse fecal samples were extracted and purified according to the  manufacturers protocol of EXT3-16S DNA Purification and PCR Amplification  Kit of Shoreline Biome (Farmington, CT), and the extended (~2,500 bp) region,  which contains 16S rRNA gene, the adjacent Internally Transcribed Spacer  (ITS) and part of the 23S gene, was amplified using barcoded primer sets in  the same kit. Equal molar quantities of the amplicons were pooled. A PacBio standard protocol (Amplicon template preparation and sequencing) was applied.