title: GSM4776568: siRNA (KMT5A) MCM3IP; Homo sapiens; ChIP-Seq
library_construction_protocol: hTERT-RPE1 cells (7.5 × 10^6 cells) were first immersed in ice cold 0.2% Triton X-100 in 20 mM HEPES (pH7.4) containing 100 mM NaCl and 300 mM sucrose for 5 min and then cross-linked with 1% formaldehyde solution (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min at room temperature (approximately 22 °C), followed by the quenching step. Dynabeads M-280 sheep anti-mouse IgG (30 μL original suspension; Thermo Fisher Scientific) and anti-MCM3 (2 μg/tube, M038-3, MBL) were used for immunoprecipitation. The ChIP-seq library of MCM3 was prepared using ThruPLEX DNA-seq Kit (Takara Bio) and sequencing was performed using HiSeq1500 (Illumina).