title: Illumina HiSeq 2000 sequencing; Small RNA-sequencing of bone marrow samples from acute myeloid leukemia patients chemoresistant (n=27) versus chemosensitive (n=20).
design: Small RNA-sequencing of bone marrow samples from acute myeloid leukemia patients chemoresistant (n=27) versus chemosensitive (n=20).
library_construction_protocol: Frozen diagnostic bone marrow RNA samples were obtained from 47 adults who had a confirmed diagnosis of acute myeloid leukemia (AML). RNA was isolated with either RNA-Bee or RLT following the protocols of the manufacturer (Bio-Connect BV, Huissen, The Netherlands). Multiplexed patient libraries (TruSeq small RNA, Illumina) were prepared.