title: GSM4775331: WT CD4TN d0 Input; Mus musculus; ChIP-Seq
library_construction_protocol: FACS sorted naive CD4+ T cells were sorted from lymph nodes. Naive CD4+ T cells were directly used for the ChIP-seq protocol for the day 0 condition, while the remaining naive CD4+ T cells were activated in Th0 culture condition for 1 day. At day 1 activated CD4+ T cells were used for the ChIP-seq protocol.  Lysed nuclei were sonicated using the Covaris E220 sonicator (Peak incident power 100, Duty factor 50, 200 cycles/burst) for 10 min. DNA fragment between 150-300bp were immunoprecipitated with anti-Ikaros antibody (#Ct, 3072, Rabbit Ab), purified and sequenced using Illumina Genome Analyzer as recommanded by the manufacturer. Library were prepared following the Diagenode MicroPlex Library Preparation Kit (C05010014) using the manifacturer instructions.