title: 16S rRNA sequence of rhizosphere microbiome of Ceanothus velutinus: native soil treated greenhouse grown samples
design: DNA was isolated from rhizosphere soil samples collected from the greenhouse grown Ceanothus velutinus plants, treated with native soil from Tony Grove area of IW region of the United States. 16S rRNA gene was PCR amplified using V4 region specific primers. Indexes were attached by a subsequent PCR followed by a clean-up using AMPureXP beads. After quantification the samples were pooled and sequenced on the MiSeq using 2x250 paired-end sequencing (Illumina).