title: WGS of Clostridium botulinum Beluga: mutant  spo0A::spo0A-wm
design: Genomic DNA extracted from 2-ml overnight TPGY liquid cultures using Wizard Genomic DNA Purification Kit (Promega, WI, USA). DNA concentration was measured using NanoDrop ND1000 spectrophotometer (Thermo Fischer Scientific, MA, USA). Genomic DNA was sequenced using the NGS platform HiSeq2000 (Illumina, CA, USA) at the sequencing unit of the Institute for Molecular Medicine (University of Helsinki, Finland),