title: Pacbio sequencing of Treponema pallidum tprK amplicon
design: The region of interest was amplified from a clonal plasmid using a HiFi polymerase (Takara). The resulting PCR product was purified with 0.6x volumes of AMPure XP Beads (Beckman Coulter) and sequenced by the University of Wisconsin-Madison PacBio Sequencing Facility.