title: Bacterial 16S rRNA gene  amplification and sequencing
design: DNA was  extracted using the PowerSoil DNA isolation kit, followed by amplification of  the V4 region of the 16S rRNA gene using standard methods. DNA libraries were  generated from the resulting amplicons using the Illumina TruSeq Nano kit  (Illumina, San Diego, CA), and amplicons were then sequenced by the Illumina  MiSeq platform using the 2- by 300-bp paired-end V3 kit (Illumina)