title: RNA-seq of mus musculus: post-natal day 0 cortex
design: RNAseq library preparation was performed starting from 100ng high-quality total RNA using the TruSeq Stranded mRNA library prep kit (Illumina, San Diego, USA). Briefly, the mRNA fraction was purified from total RNA by polyA capture, fragmented and subjected to cDNA synthesis. Barcoded DNA adapters were ligated to both ends of the double-stranded cDNA and subjected to PCR amplification. The library products were evaluated using Fragment Analyzer (Agilent Technologies), then sequenced on an Illumina NextSeq500 sequencer using 75bp single-end reads, generating ~17 million reads per sample. Quality control was conducted using the software Scythe (v0.991) and Sickle (v1.33). Transcript expression levels were then quantified in each sample by running the computer software Salmon v.1.0.0 against the reference sequence of the mouse transcriptome (Gencode M23-GRCm38).