title: GSM5840367: pL220_DMSO rep1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: An aliquot of the same samples, of the  five experimental conditions described above, used for yeast protein extract was used for total RNA isolation. Briefly, after the one hour of growth, the yeast cultures were centrifuged (5 min at 3000 rpm) and the RNA extraction was performed using the Yeast Ribopure® kit (Ambion, Invitrogen #AM1926), following manufacturer instructions.  The integrity of RNA was assessed by agarose electrophoresis.  The fifteen RNA samples have been delivered to the Leibniz Institute on Aging (FLI) in Jena where library preparation and sequencing steps have been performe RNA libraries were prepared for sequencing using standard Illumina protocols