title: GSM4775439: Mouse HSC - Control; Mus musculus; RNA-Seq
library_construction_protocol: After treatment, cells were harvested and directly subjected to library preparation with 10x Genomics platform. After treatment, 20,000 live cells from each experimental condition were subjected to 10x Genomics platform for library preparation using Chromium Single Cell 3′ Reagent Kits (v3) following the manufacturer's sample preparation guide (PN CG00054 Rev B; Fluidigm). Libraries were prepared with 10x Genomics platform, and 50ng libraries were sequenced on BGISEQ-500 platform with 28+8+91bp reads (read 1: 28bp; read 2: 91bp plus 8bp for index), at two lanes per sample.