title: RNA-Seq of Chondrolia Chalkidikis: stage3_fruit_rep1
design: Total RNA was extracted from fresh tissue material for 14 tissue types of the olive variety "Chondrolia Chalkidikis" in three biological replicates, using the Monarch Total RNA Miniprep Kit (NEB, Ipswich, MA, USA). The biological replicates per tissue type were equimolarly mixed in 2 g final RNA pools. The RNA libraries were prepared using the NeBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and library indexing was performed using the EBNext Multiplex Oligos for Illumina (Set 1 and 2) kit (, Ipswich, MA, USA), according to the manufacturers instructions. RNA library quantity and quality were assessed using the QubitTM dsDNA HS Assay Kit (Invitrogen, Oregon, USA), the 5200 Fragment Analyzer (Agilent Technology Inc., CA, USA) according to DNF-473-33 - SS NGS Fragment 1-6000bp protocol, and the KAPA library Quantification kit (KAPA Biosystems, Cape Town, South Africa).