title: GSM5226233: PDAC tissue 2; Homo sapiens; RNA-Seq
library_construction_protocol: Following resection, the tumor tissues were examined by a pathologist and then placed in cryotubes with RNAlater reagent and frozen in liquid nitrogen. Samples were transported on dry ice, and stored at -80 ºC. Total RNA was isolated using RNeasy mini (Qiagen) according to the manufacturer's protocol. The quantity and quality of the samples were assessed using NanoDrop 2000 spectrophotometer and Agilent 2100 Bioanalyzer, respectively. RNA libraries were prepared for sequencing using standard Illumina protocols