title: GSM5840337: WT_minusN_Root_1; Solanum lycopersicum; RNA-Seq
library_construction_protocol: Three independent plants were pooled to make one replication and three replications per treatment per genotype were used for RNA extraction. RNA for transcriptome profiling was extracted from the root and shoot samples harvested five hours after N treatments. Total RNA was extracted from the shoots and roots using Trizol (Invitrogen) according to the manufacturer's instructions.  After extraction, total RNA was treated with DNase (New England Biolabs) according to the manufacturer's instructions. RNA was then precipitated with 3M sodium acetate and three-times volume of 100% ethanol and resuspended in DEPC treated water. RNA was then concentrated using ZYMO RNA clean and concentrator kit (ZYMO Research) according to the manufacturer's instructions.  The integrity of RNA was accessed by the Agilent Bioanalyzer (Agilent technologies). RNA samples were submitted to the Purdue Genomics Core for RNA-Seq library preparation and sequencing on an Illumina Novaseq platform with paired-end 50bp format.