title: GSM6267637: Placental Tissue, Normal, Subject1 [NC1]; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNAs were purified from approximately 100 mg of tissue from the central regions of each placenta, using Qiagen RNeasy Purification Kit (Hilden, Germany). The qualitative assessment of RNA samples (28S/18S, 1.8-2.2; RIN, 9.5-10) were performed by Bioanalyzer (Agilent). A total amount of 1μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using TruSeq miRNA Sample Prep Kit for Illumina. Manufacturer's recommendations and index codes were added to attribute sequences to each sample.