title: GSM5194667: F3 OFC_nonstressRep1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Brain punches were vortexed for 10-20 seconds and total RNA was isolated following manufacturer's instructions using Maxwell® 16 LEV simplyRNA Tissue kit (Promega, Madison, WI). 100 ng of total RNA was subjected to ribosomal RNA (rRNA) removal using Low Input Ribominus Eukaryote System v2 (Ambion # A15027). Concentration, purity, and integrity of all RNA samples were assessed using a Nanodrop and RNA 6000 pico chip on the Agilent 2100 Bioanalyzer. An RNA-sequencing library was prepared from the rRNA-depleted RNA using Nextflex Rapid Directional qRNA-Seq Kit (Bioo Scientific, TX, USA). final pooling and sequencing (Illumina 150bp Paired End HiSeq3000, Center for Genome Research and Biocomputing at Oregon State University, Corvallis, Oregon, USA)