title: RADseq of Haliotis kamtschatkana
design: RADseq library preparation followed the BestRAD technique (Ali et al., 2016) with the restriction enzyme SbfI (5-CCTGCAGG-3 cut site). Paired-end, 100 bp sequencing was carried out by Novogene Corp. on an Illumina NovaSeq 6000. Five libraries with 96 samples each were each sequenced on an individual NovaSeq lane (5 lanes total). Samples from different populations were allocated across each library, along with a total of 25 technical replicates represented by samples sequenced in duplicate or triplicate. Raw reads were filtered and demultiplexed with the Stacks v.2 process_radtags program (Rochette, Rivera-Coln, & Catchen, 2019). Demultiplexed reads were then mapped to the red abalone (Haliotis rufescens) genome (Masonbrink et al., 2019) with the BWA mem program (Li & Durbin, 2010) using default settings.