title: NGS-HCP of human: blood
design: Libraries were prepared with 100ng of gDNA fragmented to 180-220bp                 using a Covaris E220 Series Focused- ultrasonicator (Covaris). According to the                 manufacturers protocol, each sample library was ligated with a specific index and                 after quantification and size distribution assessment with the Qubit fluorometer                 (Life Technologies) and 2200 TapeStation (Agilent Technologies) respectively.                 Libraries are pooled and captured using the SeqCap EZ Choice Library system (Roche                 NimbleGen). Captured libraries undergo appropriate quality control analysis and are                 diluted to a concentration of 4nM to process for sequencing according to                 manufacturers instructions (Illumina). The final captured library concentration for                 sequencing is 8pM with a 1% PhiX spike-in. Libraries were sequenced using the MiSeq                 v2 reagent kit to generate 2x150bp paired-end reads using the MiSeq fastq generation                 mode (Illumina).