title: GSM4819829: BisRNA-seq_Pf_G2_rep2; Plasmodium falciparum; OTHER
library_construction_protocol: The parasite pellets were washed twice with precooling 1 X PBS and then resuspended in 1 mL TRIzol (Invitrogen). Total RNA was extracted using the Direct-zol RNA Kit (Zymo Research) according to the manufacturer's instructions. mRNA enrichment from the total RNA was performed using the Dynabeads™ mRNA Purification Kit (invitrogen) as per the manufacturer's instructions. RNA bisulfite treatment and purification of converted RNA was performed using the Methylamp™ RNA Bisulfite Conversion Kit (Epigentek Group Inc.) according to the manufacturer's instructions. One ug mRNAs along with 5 ng Dhfr RNA (1:2000) as methylation conversion control were used and as input RNA. The converted RNA was subsequently used for fragmentation and library construction using the KAPA Stranded mRNA-Seq Kit (KAPA) according to the instructions provided by the manufacturer.