title: Large granular sludge with HRT of 70 minutes
design: Samples were  crushed and then centrifuged at 14, 000g, 8  for 10 min. Genomic DNA was extracted using 96 E.Z.N.A. Soil DNA Isolation Kitthe universal primer was used to amplify the V3-V4 regions of the 16S rDNA geneAfter undergoing  polymerase chain reaction (PCR), the products exceeding 440 bp were purified and quantified as 100 recommended.