title: GSM4775419: OWT1; Mus musculus; RNA-Seq
library_construction_protocol: Mouse intestine was cut longitudinally and washed three times with cold PBS. Villi were carefully scraped away and small pieces (5 mm) of intestine were incubated in 2 mM EDTA in PBS for 30 min on ice. These pieces were then vigorously suspended in cold PBS and the mixture was passed through 70 μm cell strainers. RNA libaries were prepared for sequencing using standard Illumina protocols