title: Miseq 16S faeces colon microbiota
design: Reads were de-multiplexed based on Illumina indexing system. Sequences were analyzed using QIIME 1.5.0. After filtering based on read quality and length (minimum quality = 25 and minimum length = 200), Operational Taxonomic Units (OTUs) defined by a 97% of similarity were picked using the Uclust v1.2.22 q method and the representative sequences were submitted to the RDP classifier to obtain the taxonomy assignment and the relative abundance of each OTU using the Greengenes 16S rRNA gene database21. Alpha- and beta-diversity analyses were performed using QIIME 1.5.0.