title: WGS of Escherichia coli: human adult male blood
design: A total amount of 1000g NA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEB Next Ultra DNA Library Prep Kit for Illumina(NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. Briefly, the Chip DNA was purified using AMPure XP system (Beckman Coulter, Beverly, USA). After adenylation of 3 ends of DNA fragments, the NEB Next Adaptor with hairpin loop structure were ligated to prepare for hybridization. Then electrophoresis was used to select DNA fragments specified in length. 3 L USER Enzyme (NEB USA) was used with size-selected, adaptor-ligated DNA at 37C for 15 min followed by 5 min at 95 C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The library preparations were sequenced on an Illumina NovaSeq 6000 platform and 150bp paired-end reads were generated.