title: GSM6051796: m_HO53-8H-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using NucleoSpin RNA kit (Machinery-Nagel). The quality (RIN score) and quantity of isolated total RNA samples was assessed using the DNA 5K/RNA chip for the LabChip GX (Perkin-Elmer). cDNA libraries derived from Poly-A mRNA were generated using Illumina´s TruSeq RNA v2 Sample Prep Kit. Briefly, Poly-A mRNA was isolated from total RNA samples (0.2–1 μg input) using hybridization to Poly-T beads. The Poly-A mRNA was fragmented at 94°C, and first-strand cDNA was prepared using random hexamers and the SuperScript II reverse transcriptase. Following second-strand cDNA synthesis, end repair, addition of a single A base, indexed adaptor ligation, AMPure bead purification, and PCR amplification, the resulting cDNA sequencing libraries were measured on the LabChip GX, diluted to 3 nM and stored at -20 °C.