title: Exome of Peromyscus maniculatus, Bonny Reservoir mouse
design: We extracted and quantified DNA samples for the California deer mice (Merced, CA; n=15) before shearing one g of genomic DNA to less than 500 bp with a Biorupter (Diagenode). We prepared barcoded Illumina sequencing libraries using the Meyer and Kircher (2010) protocol, then amplified libraries with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) for 6-8 cycles during the indexing PCR. Exome enrichment was conducted with a custom capture design from the SeqCap EZ Developer Libary (Nimblegen). Captures were quantified, pooled proportionally to the amount of DNA in each, and sequenced using 100bp pair-end sequencing on an Illumina HiSeq4000.