title: RNA-Seq of  homo sapiens: PC3 cells
design: mRNA was extracted using TRIzol reagents (Thermo Fisher Scientific, Waltham, MA, USA, catalog no. 15596026) according the standard protocol. Library preparation and sequencing were performed on BGISEQ-500 system by BGI Genomics Ltd (BGI). Briefly, mRNA was enriched using poly(T) oligo-attached magnetic beads and reverse-transcribed to double stranded cDNA, which was then subjected to adaptor ligation. The products were amplified by PCR and library quality was analyzed by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).