title: RNA-Seq of Mus musculus: adult blood platelets
design: Total RNA (>=1ug) for transcriptome library, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmentation of mRNA to synthesize the first strand of cDNA, RNaseH degrades the RNA strand and synthesizes the second strand of cDNA, adenylation of 3 ends of DNA fragments, adaptor with hairpin loop structure were ligated, cDNA fragments of preferentially 370~420 bp in length were selected and purified, after PCR amplification and purification, the transcriptome library was obtained.