title: GSM4818532: Pooled & sorted from AGED lungs ivCD90- CD8a+ CD44hi CD69+ D^b-NP tetramer (PE+ & APC+) T cells; Mus musculus; RNA-Seq
library_construction_protocol: Pooled & sorted from YOUNG or AGED lungs ivCD90- CD8a+ CD44hi CD69+ D^b-NP tetramer (PE+ & APC+) T cells from influenza virus infected mice (60 d.p.i.) were loaded on the Chromium Controller (10x Genomics). Single-cell libraries were prepared using the Chromium Single Cell 3' Reagent kit (10x Genomics) All scRNA-seq analyses were performed in R using the Seurat package (version 3.0) {Butler, 2018 #903}. Paired-end RNA-seq reads were aligned to the mouse mm10 genome