title: Whole mitochondrial DNA sequencing of Homo Sapien: Acute lymphoblastic leukemia (ALL)
design: Bone marrow aspirate samples were collected from the ALL patients and patients with solid tumors for staging (controls) after confirmation of the absence of bone marrow involvement. Total cellular DNA extraction from one aliquot of mononuclear cells was done using a protocol for isolation of both, nuclear and mitochondrial DNA. The enriched genomic DNA was ligated with sequencing adapters to produce a library that was sequenced on Illumina HiSeq2500 to generate 2x150 bp sequence reads greater than 10,000 X sequencing coverage depth.