title: GSM6051845: XIST KD Replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from WT A431 cells, pCAS-treated A431 cells, and XIST knockdown A431 cells using RNeasy Mini Kit (Qiagen, cat # 74104) with on-column DNase digestion using the RNase-Free Dnase Set (Qiagen, cat # 79254). Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus with manufacturer's protocol