title: GSM6051574: SMF001 CFP DMSO Replicate #1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted by TRIzol (Thermo Fisher Scientific, 15596026). About 200 ng total RNA was treated using Kapa rRNA depletion reagents to remove ribosomal RNA, then converted into cDNA libraries using Kapa RNA HyperPrep Kit with RiboErase (HMR). After end repair, dA-tailing, and adapter ligation, each cDNA library was purified and enriched by 11 cycles of PCR amplification.