title: RNA-seq of mouse: male spleen
design: RNA-Seq libraries were prepared using Roche Kapa RNA HyperPrep with Riboerase. RNA sample RIN was equal to 10. Input amounts were split into 2 batches, low input 11.2 ng and standard input 30 ng. Library amplification cycles were 14 for low input and 12 for standard input. Libraries were cleaned using standard AMPure bead protocols and balanced using fragment analysis (Agilent 5200) and DNA quantitation (Qubit). The library pool was sequenced on an Illumina NovaSeq 6000 on an S2 flow cell as 2x 150 bp reads.