title: JJN3 shRELA2 knockdown n2
design: For RNA extraction 5x105 YFP DsRED double positive cells were sorted at day 3 post Doxycycline induction. RNA was extracted using a standard Trizol based method and followed by DNase treatment (Thermo Fisher). RNA quality was verified using a tapestation (aligent) prior to mRNA polyA selected library preparation. RNA was sequenced on a NextSeq500 (Illumina) with a read length of 75 base pairs, single end sequencing.