title: RNA-seq of banana: root
design: 1 g total RNA was used for following library preparation. The poly(A) mRNA isolation was performed using Oligo(dT) beads. First strand cDNA and the second-strand cDNA were synthesized. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated. Then libraries with different indexs were multiplexed and loaded on an Illumina HiSeq/ Illumina Novaseq/ MGI2000 instrument for sequencing using a 2x150 paired-end (PE) configuration according to manufacturers instructions.