title: GSM5896715: Y3F_1; Mus musculus; RNA-Seq
library_construction_protocol: Sequencing libraries were prepared directly from the freshly sorted lysed cells, without a previous RNA extraction step. RNA reverse transcription and cDNA amplification were held using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing from Clontech Takara. The Illumina "Nextera XT" kit is used for the preparation of the libraries from the amplified cDNA. Libraries were sequenced in single-end mode with a read length of 51 base pairs with an average of 20 million reads for each sample by the Illumina HiSeq 2500 sequencer in the Genomic Unit of Centro Nacional de Investigaciones Cardiovasculares (CNIC).