title: C. cajan leaf 4
design: Small RNA sequencing (SmRNA) libraries were prepared with NEXTflex Small RNA Sample Preparation protocol at Genotypic Technology Pvt. Ltd., Bangalore, India. 500 ng of Qubit quantified total RNA was used as starting material. 3 adapters were ligated to the specific 3OH group of micro RNAs followed by ligation of 5 adapter. Adapter ligated fragment was reverse transcribed with M-MuLV Reverse transcriptase by priming with reverse transcription primers. cDNA thus formed was enriched and barcoded by PCR amplification.