title: RNA-seq of foxtail millet grain
design: Total RNA was isolated using a Quick RNA Isolation kit (Takara Corporation, Dalian, China) , and was assessed using  NanoPhotometer spectrophotometer (IMPLEN, CA, USA) and an RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total of 3 g of RNA per sample was used as input material. The construction of RNA-seq libraries and sequencing of the libraries were performed following standard methods by Novogene Life Sciences Pvt. Ltd., Beijing, China. The libraries were sequenced on an Illumina HiSeq platform, and 150 bp paired-end reads were generated