title: 16S MiSeq of Danaus Plexippus: larval gut curassavica control parasite load low
design: The 16S rRNA gene was amplified with barcoded dual-indexed primers 515F and 806R specific to the V4 region. The PCR cycle consisted of 2 min at 95C, followed by 30 cycles of 95C for 20 s, 55C for 15 s, and 72C for 5 min, followed by 72C for 10 min. PCR reactions were normalized, pooled, and quantified for amplicon library preparation. Libraries were sequenced on an Illumina MiSeq platform with 250bp paired ends.