title: GSM4776587: UNC_P40_WT1_CTX_BS; Mus musculus; Bisulfite-Seq
library_construction_protocol: Mouse cortices were collected and DNA was extracted using Qiagen Blood and Tissue kit. Genomic DNA was extracted from mice cortex with the DNeasy Blood & Tissue Kit (Qiagen) and sonicated with a Covaris S2 instrument to generate 200 bp fragments. Unmethylated lambda DNA (0.1%–0.5%; Promega D1521) was spiked in to assess conversion rates. Whole genome bisulfite-sequencing was performed  as described (Stroud et al., 2017).