title: GSM6102610: S100a10_bacTRAP_Flx_Sh_anxious_input_Rep2; Mus musculus; RNA-Seq
library_construction_protocol: Cortex was dissected and homogenized and tagged polysomes were immunopurified with monoclonal anti-EGFP antibodies bound to biotinylated-Protein L (Thermo Fisher) coated streptavidin-conjugated magnetic beads (Life Technologies). RNA from Immunoprecipitated (IP'ed) and input samples were purified with the RNeasy plus micro kit (Qiagen) following manufacturer's protocol. 2.5 ng RNA from TRAP IP or input was reverse transcribed using the NuGEN Ovation RNA-Seq System V2. Libraries for sequencing were generated using 500ng of cDNA using Illumina TruSeq RNA Sample Preparation Kit v2 following manufacturer's protocols. Samples were sequenced in two independent lanes on an Illumina NextSeq 500 sequencer using the 75-bp paired-end sequencing.