title: GSM5896565: pbmc LRBA patient [P2_LRBA]; Homo sapiens; RNA-Seq
library_construction_protocol: The scRNA-seq libraries were generated using 10X Chromium Single Cell 3′ Library & Gel Bead Kit (v.2 for batch 4, v.3 for batch 1 and 2 and v.3.1. for batch 3) according to the manufacturer's protocol. Cells were counted, diluted at 1000 cells/µL in PBS+0,04% and 20 000 cells were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. After reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR. Following fragmentation, end repair and A-tailing, sample indexes were added during index PCR. The purified libraries were sequenced on a Novaseq (Illumina) with 26 cycles of read 1, 8 cycles of i7 index and 98 cycles of read 2 (for kit v.2.) or with 28 cycles of read 1, 8 cycles of i7 index and 91 cycles of read 2 (for kit v.3 and v.3.1).