title: GSM4819721: Naive_MEL1_P20plus3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the Agencourt RNAdvance Cell v2 (Beckman Coulter) system following the manufacturer's instruction with one additional DNAse (NEB) treatment step. RNA amounts and RINe scores were assessed on a TapeStation using RNA Screen Tape (Agilent), and 500 ng of total RNA were used per sample to generate RNAseq libraries. ERCC ExFold RNA Spike-In mixes (Thermo Scientific) were added as internal control. Libraries were prepared using the TruSeq Stranded mRNA library prep kit (Illumina), using TruSeq RNA unique dual index adapters (Illumina). Libraries were quantified by qPCR on a CFX96/C1000 cycler (Biorad) and sequenced on a Novaseq 6000 (Illumina) for 2x 53 bp as paired-end.