title: Metagenome of Vitis vinifera: Xylella fastidiosa
design: Sequencing libraries were prepared following ONT (Oxford, UK), protocols. First, DNA fragments were repaired using the FFPE Repair Mix (New England Biolabs, Inc., Ipswich, MA; M6630), the repaired samples were barcoded using the Native Barcoding kit (ONT Ltd., EXP-NBD114), the barcoded samples were mixed and ligated with the adapter using the Ligation Sequencing Kit (ONT Ltd., SQK-LSK109), and the final product was prepared as the loading library using Ligation sequencing kit. The products from each of the steps were monitored by using NanoDrop ONE . The library was loaded on the MinIon Flow Cell by using the Flow Cell Priming Kit (ONT Ltd., EXP-FLP002), and ran using the MinKNOW software (version 4.2.8) for 72 hours.