title: Amplified V1-V2 region of 16S rRNA gene of bacterial communities from the western North Atlantic
design: DNA libraries were prepared using the Nextera XT Index Kit, v2 set A (Illumina). The V1-V2 hypervariable region of the 16S rRNA gene was amplified using the primers 8F (5- AGA GTT TGA TCC TGG CTC AG-3) and 338R (5- GC TGC CTC CCG TAG GAG T-3) with the Illumina-specific forward primer overhang adapter (5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG-3) and reverse overhang adapter (GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G), and sequenced using Illumina MiSeq PE 2x250.