title: GSM5194600: CEF4_1; Gallus gallus; RNA-Seq
library_construction_protocol: RNA was extracted from primary and anchorage-independent cell lines using RNeasy Micro kit (Qiagen, USA) according to the manufacturer's directions. Library preparation and RNA sequencing were performed by Syntezza Bioscience (Israel). Library construction was conducted using NuGEN TrioRNA-Seq kit (Tecan, USA) and sequenced on Illumina HiSeqX with paired-end, 150 bp reads using the High Output V2 Kit.