title: GSM5934974: KO2 spermatocytes; Mus musculus; RNA-Seq
library_construction_protocol: Primary spermatocytes were collected using flow cytometry via Hoechst staining of single cell suspension of testes and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 2 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols