title: ChIP-Seq of D. melanogaster: S2 Cells
design: Chemically cross-linked S2 cells were lysed and sonicated to shear DNA to 300-500 bp fragments. Lysate was treated with F77 (Stwl) antisera at 1:50 dilution. IP complexes were immunoprecipitated with Invitrogen DynabeadsTM Protein A for Immunoprecipitation (10001D). Beads were washed in various buffers before IP complexes were eluted from them. IP complexes were reverse-crosslinked and treated with RNAse A and Proteinase K prior to DNA extraction. DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB# E7645), using Ampure XP beads for cleanup, without size selection.