title: RNA-Seq of Ictalurus furcatus: testes tissue
design: mRNA was purified using poly-T oligo-attached magnetic beads and cDNA was synthesized. Final cDNA libraries were quality controlled using Qubit and real-time PCR before sequencing was performed on an Illumia platform (Novoseq 6000, HWI-ST1276, Illumia, San Diego CA, USA). Index-coded samples were clustered using a cBot Cluster Generation System with a TruSeq SR Cluster Kit v3-cBot-HS. Sequencing was then performed on an Illumina sequencing instrument (Novoseq 6000, HWI-ST1276, Illumia, San Diego CA, USA) where 150 bp paired-end reads were generated.