title: Whole genome sequencing of Acinetobacter baumannii
design: DNA was extracted from overnight cultures of wild-type and mutant A. baumannii isolates resistant to tetracycline, doxycycline or tigecycline in the presence of PBT2 + Zn using the Wizard Plus SV Minipreps DNA purification System (Promega) as per the manufacturer's instructions. Paired-end Illumina DNA libraries were prepared using the Nextera DNA flex Library Prep Kit (Illumina, Australia) with a modification of the starting input of 5?L gDNA with samples >20ng/?L. WGS of pooled libraries was performed using the Illumina MiniSeq and the high Output Reagent kit (300 cycles).