title: scRNA_seq of rat: normal murine middle ear mucosa
design: Ten (i.e., 20 ME bullae) male rats (Rattus norvegicus; 810 weeks) were divided randomly into the AOM group (treated with LPS) and control (untreated) group (5 rats for each group).  Their middle ear mucosa was harvested  for single-cell RNA sequencing via . Cell clustering and trajectory analyses were undertaken using Seurat and Monocle 3 packages in R software. Pathway analyses were done by gene set enrichment analysis (GSEA). Cellcell interactions were inferred by CellChat. Cell scores were calculated to identify cells with dual-feature.