title: RNA-seq of microbe
design: The PCR procesure was set at: 5 min at 94C; 30 cycles of 1 min at 94C, 1 min at 55C, and 1.5 min at 72C; followed by 5 min at 72C. The PCR products were detected via 1% agarose gel electrophoresis and Nanodrop 2000, and purified by EZNA Gel Extraction Kit (Omega,USA). Sequencing libraries were generated using NEBNextUltraDNA Library Prep Kit for Illumina (New England Biolabs, MA,USA) following manufacturers instruction.