title: RNA-Seq of Strongyloides stercoralis free-living male
design: S. stercoralis adult free-living males were isolated via the Baermann technique and cleaned via migration through agarose. Total RNA was extracted using TRIzol. Libraries were constructed using 500 ng of total RNA with a RIN >8.0 and the Illumina TruSeq RNA Sample Prep Kit with oligo-dT pull-down. Library concentration was determined using the Kapa SYBR Fast qPCR kit; libraries were pooled in equimolar ratios and sequenced on a single flow-cell lane.