title: Liver RNA-Seq
design: Liver total RNA samples (750 ng) were synthesized into cDNA sequencing libraries using the Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold kit (Illumina, San Diego, California, USA) in the epMotion liquid handling workstation (Eppendorf, Hamburg, Germany). The cDNA libraries were pooled and sequenced on the Illumina NextSeq 500 sequencer at 75 nucleotide length single end reads to reach an average of 30 million reads per sample.