title: GSM4776936: MCF10A-KI-BYL719-11; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the QIAGEN RNeasy Kit and retrotranscription was performed using the iScript cDNA synthesis kit from Bio-Rad, following manufacturer's instructions. cDNA was amplified by real time quantitative PCR in a ViiA 7 Real-Time PCR system, using SYBR Select Master Mix from Applied Biosystems RNA from MCF10A cells was sequenced using Illumina HiSeq 2000 instrument at 2 X 150 nucleotide paired-end reads.  RNA from MEF cells were sequenced using Illumina HISeq 2000 instrument at 2 x 100 nucleotide paired-end reads.