title: RNA-Seq of Chinese cherry amended with washed tobacco biochar
design: Briefly, total RNA was isolated from samples using RNA prep Pure Polysaccharide Polyphenol Plant Total RNA Extraction Kit (Tiangen, Beijing, China) in accordance with manufacturers instructions. Quality and concentration of extracted RNA were checked by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA) and Nanodrop 2000 micro-spectrophotometer (Thermo Fisher Scientific, Waltham, USA), respectively. Whereafter, mRNA was enriched using magnetic beads with Oligo dT and fragmented into shorter sequences. The cDNA was reverse transcribed with random hexamer primers follwed by synthesis of the complementary sequences. Subsequently, adapters were ligated to both ends of these double-stranded DNA fragments. After amplification by PCR, products were subjected to high-throughput sequencing by Illumina HiSeq 4000 (Shanghai Majorbio Bio-pharm Technology Co. Ltd., China).