title: 16S rRNA sequencing of effluent from livestock market.
design: The amplicon library was prepared using  the 16S Metagenomic Sequencing Library preparation protocol (Part # 15044223 Rev. B) of Nextera XT Index Kit (Illumina Inc.). Primers for the amplification of the V3-V4 hyper-variable region of 16S rDNA gene of bacteria and archaea were designed by Xcelris Labs Limited.