title: RNA-seq of mice liver
design: First total RNA was extracted through the TRIzol method. Then agarose gel electrophoresis was conducted for RNA quality inspection, and finally the construction of a transcriptome library was conducted. AMPure XP beads were used to screen 200 bp cDNA sequences, PCR amplification was performed, AMPure XP beads were used again to purify the PCR products, and the library was finally obtained.