title: Bacterial 16S rRNA gene  amplification and sequencing
design: DNA was extracted using the  PowerSoil DNA isolation kit, followed by amplification of the V4 region of  the 16S rRNA gene using standard methods. DNA libraries were generated from  the resulting amplicons using the Illumina TruSeq Nano kit (Illumina, San Diego,  CA), and amplicons were then sequenced by the Illumina MiSeq platform using  the 2- by 300-bp paired-end V3 kit (Illumina)