title: GSM5620710: MRC5 cells, H3K4me3 DFF ChIP-Seq (Sample 3); Homo sapiens; ChIP-Seq
library_construction_protocol: Rapid nuclei isolation steps were performed on wet ice using ice-cold buffers as previously described (Nilson et al. 2017; Ball et al. 2019). Following DFF digestion, nuclei were lightly sonicated for 20s and the soluble material was immunoprecipitated with 2.5 µg of antibodies. Library construction is detailed in the manuscript.