title: 16S rRNA sequencing: colorectal cancer
design: We extracted genomic DNA from human tissue samples. A negative control and a positive control (ZymoBIOMICS Gut Microbiome Standard; replicated in three wells with unique adapters) were included in library preparation. We performed PCR amplification targeting the V3/V4 regions of the 16S rRNA gene. The amplicons underwent 1.8X Ampure bead cleanup to remove excess primers from the reaction. Finally, the amplicons were tagged with Nextera sequencing adapters through PCR.