title: Comparison of Methanogens diversity between cattle and  Buffaloes fed on similar diet and kept in same environmental conditions
design: Amplicon libraries were prepared  using the Nextera XT Index Kit (Illumna Inc, USA). Archaea specific primers (forward:  ACGGGGYGCAGCAGGCGCGA; reverse:  GGACTACVSGGTATCTAAT) were used for the amplification of 16s rDNA.  Amplicon libraries were purified by  AMPureXP beads and quantified using Qubit  fluorometer. The amplified libraries were analyzed on 4200 Tape  Station system (Agilent Technologies)  using D1000 Screen Tape.