title: GSM4775624: FR_Mock _0hpi_rep3; Solanum lycopersicum; RNA-Seq
library_construction_protocol: Total mRNA was isolated by using mRNA pull down method with magnetic streptavidin beads (Townsley et al., 2015) Non-strand specific library according to Townsley et al. (2015) with minor modifications. Total mRNA was isolated from ground tomato leaf tissue using biotin oligo primers and magnetic streptavidin beads. mRNA pellets were washed and fragmentated. cDNAs were synthesized by using reverse transcriptase, PolI, RNAseH and T4 polymerase. cDNAs were washed and ligated to non-strand specific adapters and enriched by PCR. Libraries were size-selected (350bp) and eluted in Tris-HCl buffer. Libraries were sequenced using 1 x 75 pb high output Illumina NEXTseq500.