title: GSM4776925: Individual 25 Severe symptoms – s7; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA extraction was performed and assessed using the IVD-compliant (In Vitro Diagnostic device) automated magnetic-bead-based system magLEAD 12gC (Precision System Science Co., Ltd.), following the manufacturer's instructions and was followed by storage at -80o C. sRNA-Seq libraries were prepared using QIAseq miRNA Library Kit (Qiagen), employing Unique Molecular Indices (UMIs) to control PCR amplification bias. Quality control of the sequencing libraries was performed with the 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent).