title: RNA-SEQ encompassing the NS3/4a genes of hepatitis C virus  from plasma of a patient with chronic infection
design: Amplicons of 2223 bp, encompassing the NS3/4a viral genes of hepatitis C virus, (nt 3466-5689 according to H77 reference genome, GenBank: AF009606), covering epitopes NS31073, NS31406 and NS31436  encoding  regions were tagmented using Nextera XT Sample preparation Kit (Illumina). Sequencing was performed on MiSeq (Illumina) platform using MiSeq Reagent v3 kit 2x300 bp, (Illumina).