title: WTS of breast cancer samples
design: WTS of breast cancer samples
library_construction_protocol: Thirty-one women diagnosed with operable breast cancer were recruited in this study. Eighteen patients were administered a single dose of 500 mg of HP within 15 days prior to surgery (Exposed Group), while 13 patients were not subjected to this intervention (Unexposed Group). From each patient, multiple core biopsies were collected immediately before administration of HP (for patients in exposed group) or at the start of surgery (for patients in unexposed group) as pre-surgery samples. Multiple cores obtained from the fully resected tumour constituted the post-surgery sample in Exposed and Unexposed groups. From 31 women diagnosed with operable breast cancer, 31 tumors and 10 adjacent normal samples were obtained. Multiple core biopsies were collected from these patients pre- or post-surgery for the respective groups of unexposed or exposed to intervention of hydroxyprogesterone. Total RNA was extracted from the tissue samples stored in RNA later using Trizol and the RNA purification was performed using PureLink RNA Mini Kit. Libraries for RNA Sequencing were prepared following Illumina True-Seq RNA Sample Preparation Kit