title: GSM4819633: E12 chick retina, sample 1; Gallus gallus; RNA-Seq
library_construction_protocol: Cells were counted, resuspended in PBS with acetylated BSA, and processed with the Chromium Next GEM Single Cell 3' Library Construction Kit (version 2) (10x Genomics, Pleasanton, CA; Zheng et al., 2017) Single cell libraries were made with Chromium 3' v2 platform (10X Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, single cells are partitioned into oil droplets containing single oligonucleotide-derivatized beads followed by cell lysis, barcoded reverse transcription of RNA, amplification, shearing, and attachment of 5' adaptor and sample index oligos. Libraries were sequenced on the Illumina HiSeq 2500 (Paired end reads: Read 1, 26bp, Read 2, 98bp)