title: Metagenomic Sequencing: Amplicon of 16s rRNA
design: Whole genome sequencing library preparation was performed using Nextera XT DNA Library Preparation protocol.The genomic DNA was tagmented using Amplicon Tagment Mix provided in the Nextera XT Kit. The adapter tagged DNA was subjected to 12 cycles of Indexing-PCR to enrich the adapter-tagged fragments. Quantification and size distribution of the prepared libraries were determined using Qubit Fluorometer and Agilent Bioanalyzer 2100 respectively. Samples were loaded into an Illumina MiSeq v3 600 cycles cartridge. The flow cell and the PR2 buffer was loaded onto the machine and the run was performed according to standard Illumina protocol. The sequencing run was performed in paired end mode with 288 bp read length for paired end reads.