title: seeded date plum transcriptome data
design: (1) Enrichment of eukaryotic mRNA by magnetic beads with Oligo (dT)(2) mRNA was randomly interrupted by Fragmentation Buffer(3) Using mRNA as template, the first CdNA5 was synthesized with random hExamersAnd then add the buffer solution, DNTPs, RNase H and DNA Polymerase I to synthesize the second cDNA strandAMPure XP beads were used to purify cDNA(4) The purified double-stranded cDNA was repaired at the end, A tail was added and the sequencing connector was connected, and then AmPure was usedXP Beads do segment size selection(5) Finally, the cDNA library was obtained by PCR enrichmen