title: Sample_Rev
design: The genomic DNA of samples was respectively digested using the restriction enzymes, and the obtained fragments were ligated with barcodes, and then they were amplified by PCR.0.3~0.6 g genomic DNA was digested with the restriction enzyme in order to obtain a suitable marker density; each end of digested fragment was respectively ligated with P1 and P2 adapter; tags containing both P1 and P2 adapters were amplified through PCR.