title: shotgun metagenomic sequencing of fecal sample: neurologically healthy control subject
design: DNA was isolated from an aliquot of stool sample using the Qiagen DNeasy PowerSoil Pro high-throughput kit on the QIAcube high-throughput platform (Qiagen, Germantown, MD, USA) according to the manufacturers protocol. Isolated DNA was quantified by Qubit. The sequencing libraries were prepared by using the Illumina Nextera XT transposase system. Libraries were sequenced on the Illumina NovaSeq 6000 instrument aiming for 40M reads per sample, following paired end 150bp sequencing protocol.