title: GSM5889075: Rectus Abdominis 59F; Homo sapiens; RNA-Seq
library_construction_protocol: Freshly harvested human muscle was  stored in DMEM with 30% FBS at 4°C overnight. Muscle was digested, erythrocytes were lysed, and hematopoietic and endothelial cells were depleted with magnetic column depletion. Viable cells were depleted for CD31, CD34, and CD45 expressing cells. Cells that remained after depletion were sorted for CXCR4+/CD29+/CD56+ and collected for single cell rna sequencing (Garcia et al., 2018; Garcia et al., 2017). To capture individual cells, we utilized the Chromium Single Cell 3' Reagent Version 3 Kit from 10X Genomics (Zheng et al., 2017). Libraries were prepared with the Chromium Single Cell 3' Reagent Version 3 Kit.