title: Viral metagenome of brain tissue
design: Brain tissue (0.1g) from 82 year old deceased individual (ID 04-05) was homogenised in 300l of SM Buffer (0.1M NaCl, 50mM Tris/HCl-pH 7.4, 10mM MgSO4) and viral DNA extracted from the supernatant using the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). Circular DNA was preferentially amplified by rolling circle replication amplification (RCA) with the TempliPhi kit (GE Healthcare, USA). The resulting RCA DNA was used to generate 2x100bp libraries using the Nextera DNA flex kit (Illumina, USA)