title: RNA-Seq of Danio rerio: adult whole fish
design: Total RNA was extracted from each zebrafish sample according to the manufacturer's instructions. The mRNA was enriched using beads with oligod (T) and then mixed with the fragment buffer to fragment the mRNA. Two strands of cDNA were then synthesized successively using random primers and a series of substrates and polymerases. Then, the sequencing adapter was ligated to the cDNA fragment for PCR amplification on S1000TM Thermal Cycler (Bio-Rad Laboratories, California, USA). And the Agilent 2100 Bioanaylzer and Qubit DNA HS Assay Kit (Invitrogen, California, USA) were used for quality control and quantification of the constructed library.