title: GSM5226540: fibroblast transcriptome rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA extraction, cells were lysed in Trizol and total RNA was prepared using Direct-zol RNA miniprep kit (Zymo Research) following the manufacturer's instructions that include an on-column DNase digestion step. RNA quality was assessed on a Bioanalyzer, and libraries were prepared using Illumina Ribo-zero kits according to the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSEQ6000 (100bp paired-end reads). RNA-seq of rRNA-depleted RNA