title: GSM5580323: Refed 30 min trl-1(cas1078) rep1 Ribo-Seq; Caenorhabditis elegans; OTHER
library_construction_protocol: Worms were flash-frozen and stored in liquid nitrogen. The sample pellets were crushed into powder in a motar filled with liquid nitrogen prior to the experiments. RPF was harvested using RNase I digestion Libraries were performed as Ingolia et al. 2012 published protocol described.