title: 16S sequencing of intestinal mucosa
design: 1) Add the official Illumina linker sequence to the outer end of the target region by PCR; 2) Use the gel recovery kit to cut the gel to recover the PCR product; 3) Elute with Tris-HCl buffer and detect by 2% agarose electrophoresis; 4) Hydrogen Sodium oxide denatures, producing single-stranded DNA fragments.