title: PacBio WGS of Epinephelus malabaricus: juvenile
design: PacBio sequencing - DNA extraction: NucleoBond HMW DNA (MACHEREY-NAGEL); Library prep: SMRTbell Express Template Prep Kit 2.0, SMRTbell Enzyme Cleanup Kit; Sequencing Kit: Sequencing primer v2, Sequel II Binding Kit 2.0, Sequel II Sequencing Kit 2.0; SMRT Cell : SMRT Cell 8M; Loading:  60-100pM, Diffusion loading; Sequencer: Sequel II System; Analysis software:  SMRT Link v9.0