title: Exome of Peromyscus maniculatus, Bonny Reservoir mouse
design: We extracted and sheared DNA to ~300 bp using a Covaris E220 Focused Ultrasonicator. Genomic libraries for each individual were prepared using 200 ng of sheared DNA with a NEBNext UltraII kit and unique index following manufacturers protocols. We pooled batches of 24 indexed libraries prior to target enrichment and PCR amplification following the NimbleGen Seq Cap EZ protocol (Roche). Each capture pool of 24 individuals was sequenced with 100 bp paired-end sequencing on an Illumina HiSeq 4000.