title: RNA-Seq of Temsirolimus treated neurons
design: RNA was converted into cDNA by using QuantSeq 3mRNA-Seq Reverse (REV) Library Prep Kit (Lexogen, Vienna, AU) according to manufacturer's instruction to generate compatible library (2.5 pM) for Illumina sequencing. RNA sequencing was performed using a high output reagent cartridge v2 (75 cycles; Illumina, San Diego, USA) with a custom primer (0.3 M) provided by Lexogen on an Illumina NextSeq 500 device