title: GSM4775398: KO_SAT; Mus musculus; RNA-Seq
library_construction_protocol: Cells were prepared according to 10x Genomics® Single Cell Protocols Cell Preparation Guide, followed by MACS Dead Cell Removal Kit 130-090-101 and RBC removal single-cell transcritpome caputred by 10X 3' Reagent Kiits (v3) After running on 10X Chromium Single Cell Controller, gel beads-in-emulsion (GEMs) were generated. The barcoded and full-length cDNAs were produced after incubation of the GEMs and amplified via PCR. Library was qualified by Agilent Bioanalyzer 2100 and quantified by real-time PCR on QuantStudio3. Sequenced was done with Illumina NextSeq 550 System using High Output Kit v2.5 (50,000 reads per cell). R1 (barcode and UMI), R2 (3' cDNA reads), I1 (index files)