title: GSM4819751: RNA-seq for CD127low monocytes D41; Homo sapiens; RNA-Seq
library_construction_protocol: Buffy coat from healthy blood donor was diluted by PBS, and PBMC were isolated by Ficol gradient. CD14+ monocytes were isolated from PBMC with anti-CD14 magnetic beads (Miltenyi Biotec). CD14+ monocytes were treated with LPS for 6 h, and CD127high and CD127low monocytes were sorted by FACS. For ATAC-seq, cells were washed by PBS and 50000 cell were re-suspended in 50 μL of lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.5% NP-40) and nuclei were digested with Tn5 enzyme. DNA libraries were prepared for sequencing using TruePrepTM DNA Library Prep Kit V2 for Illumina protocols. Instrument Hiseq X ten.  For RNA-seq, RNA was extracted using TRIzol, and total RNA was polyA enriched. RNA libraries were prepared for sequencing using TruSeq mRNA-seq Sample Preparation Kit(Illunima). Instrument BGISEQ-500RS.