title: RNA-Seq of Ochlerotatus hexodontus: adult female
design: Mosquito homogenates were filtered through a 0.8 m polyethersulfone (PES) filter and treated with micrococcal nuclease and benzonase. RNA was then extracted using TRIzol according to the manufacturers instructions. The RNA samples were treated with DNase I and purified with Agencourt RNA Clean XP magnetic beads. Ribosomal RNA was removed using a NEBNext rRNA depletion kit according to the manufacturers protocol, followed by amplification using a whole transcriptome amplification WTA2 kit. The sequencing libraries were prepared using a Nextera XT kit and sequenced using the Illumina Miseq platform and v2 reagent kit with 150 bp paired-end reads.