title: GSM6050694: Whole Blood, mixed, scRNAseq; Rousettus aegyptiacus; RNA-Seq
library_construction_protocol: Peripheral blood was drawn from sedated animals. After whole blood or PBMC preparation, cells were filtered through 70µm Flowmi® cell strainers. Platelets were depleted by cell sorting using a 100µm nozzle, the lowest flow rate and the single cell setting of the cell sorter FACSAria Fusion (Becton Dickinson). Sorted cells were centrifuged at 400×g and 4°C for 15min. After centrifugation, the cell count was set at 1000cells/µl in 1x PBS + 20µg/ml UltraPure™ BSA. Samples were loaded on separated wells of a Chromium Next GEM Chip G before running on a Chromium controller. Reverse transcription and cDNA library preparation were performed according to the manufacturer's instructions (GEM Single Cell 3' Reagents Kits v3.1, 10x Genomics).