title: Whole mitochondrial DNA sequencing of Homo Sapien: healthy controls
design: Peripheral blood samples were collected from healthy adults and sibling controls. Total cellular DNA extraction from one aliquot of mononuclear cells was done using a protocol for isolation of both, nuclear and mitochondrial DNA. The enriched genomic DNA was ligated with sequencing adapters to produce a library that was sequenced on Illumina HiSeq2500 to generate 2x150 bp sequence reads greater than 10,000 X sequencing coverage depth.