title: 16S amplicon : gut samples from fish
design: Gut samples was collected from fish from Qinghai Lake, DNA was colletced  using the FastDNA Spin Kit for Soil (MP BIO, Solon, OH, USA) according to the manufacturers protocol. The DNA concentration was measured using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany). The barcoded polymerase chain reaction (PCR) primers 515F/806R targeting the V4 region was used for the 16S rRNA amplification