title: GSM5856465: DKO Heart ATAC 1; Mus musculus; ATAC-seq
library_construction_protocol: 50-70,000 flow-sorted live endothelial cells were used to prepare the ATAC-seq library by preparing nuclei and applying a transposase reaction with 12 cycles of PCR amplification The library was purified using SPRI beads (double-sided selection, 200-500bp fragment size). We utilized Illumina Hi-Seq system to sequence libraries