title: PAP-alpha RNAi X1 cells Rep1
design: From the PAP-alpha RNAi animals (post 6 dsRNA feeds), X1 cells were sorted based on nucleus to cytoplasmic ratio. RNA was extracted from these sorted cells using Trizol. We used Qubit to check RNA concentration and bio-analyser to check quality of isolated RNA. We did polyA selection to enrich for mRNAs and subjected to transcriptome library preparation. Transcriptome library was prepared using NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (Catalog no-E7765L) kit and sequenced in Illumina HiSeq 2500 machine. All the samples were sequenced (as single-end) in biological replicates.