title: GSM6098678: MAD3; Homo sapiens; RNA-Seq
library_construction_protocol: The RNA samples were extracted from GC tissues and cells using RNA Isolation TRIzol Reagent. Complementary DNA (cDNA) were synthesized from RNA sample using 5X All-In-One RT MasterMix. The amplification reaction volume was 20 μL and contained 10 μL EvaGreen 2X qPCR MasterMix, 1 μL cDNA, 2 μL amplification primers, and 7 μL ddH2O. The lncRNA library was constructed by taking 3 μg of total RNA for each sample as the starting amount. Use Ribo-Zero™ GoldKits to remove rRNA in the sample, and select different index tags to build the library according to the instructions of NEB Next Ultra Directional RNA LibraryPrep Kit for Illumina.