title: GSM4775336: Rep1 TH0 d2 WT [ATAC-Seq]; Mus musculus; ATAC-seq
library_construction_protocol: FACS sorted naive CD4+ T cells were sorted from lymph nodes and spleen of WT and Ikaros TKO mice. Naive CD4+ T cells were directly used for the ATAC-seq protocol for the day 0 condition, while the remaining naive CD4+ T cells were activated in Th0 culture condition for 1 or 2 days. At day 1 and 2, live cells (DAPI-) were sorted and used for the ATAC-seq protocol. Libraries were prepared as described in Buenrostro et al., 2013.