title: GSM5512911: Gxp4 MT Heart 2; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was extracted using TRIzol Reagent (Invitrogen) recommended by manufacturer. Libraries were prepared with the QuantSeq 3' mRNA-Seq Fw. Library Prep Kit (Lexogen) and sequenced to 10M raw reads on an Illumina HiSeq 2500 machine, following the manufacturer's protocols. 3' Poly(A) RNA-seq RNA: total RNA was extracted using TRIzol Reagent (Invitrogen) recommended by manufacturer. Libraries were prepared with the QuantSeq 3' mRNA-Seq Fw. Library Prep Kit (Lexogen) and sequenced to 10M raw reads on an Illumina HiSeq 2500 machine, following the manufacturer's protocols.