title: WGS Streptomyces_sp_09MW18-IS
design: For whole-genome shotgun sequencing, the genomic DNA was extracted using the UltraClean microbial DNA isolation kit (Mo Bio Laboratories, Inc.) and sequenced using Illumina technology. The sequencing was done at Wrights lab, Huntingdon, PA. For Illumina, library preparation was performed using Illumina's Nextera XT library prep kit. Illumina adapters and unique 8-bp dual indexes were ligated to 1ng of DNA with no protocol adaptations. The library quality was checked on an Agilent Bioanalyzer 2100 with a high-sensitivity DNA analysis kit. The library was normalized, multiplexed, pooled, and subsequently purified on a 2% agarose gel with GelStar stain (Lonza) to aid with visualization. DNA was size selected between 250 and 400bp for extraction using a QIAquick gel extraction kit (Qiagen) for sequencing. The final purified library was then quality checked and quantified on a Bioanalyzer, followed by sequencing using 150-bp paired-end chemistry on a NextSeq 550 platform.