title: GSM4819301: PBMC untreated donor D; Homo sapiens; RNA-Seq
library_construction_protocol: mRNA was extracted using the PureLink RNA Mini Kit (Invitrogen) and RNA concentration quantified by Qubit (Invitrogen) and quality assessed by Bioanalyzer (Agilent). PolyA RNA libraries were generated according to the Illumina TruSeq Stranded mRNA protocol using 400 ng total RNA. Libraries were pooled and sequenced by Illumina NextSeq500 sequencer in high output mode with v2.5 chemistry, using 75 base-pair single-end reads. PhiX spike-in was included at 1% for quality control.