title: HiC illumina genome of Helicoverpa zea pupa
design: The HiC libraries were prepared as described in Erez Lieberman-Aiden et al. (2009). The process involves the fixation of genomic DNA as chromatin in vitro with formaldehyde followed by extraction. The fixed chromatin is digested with DpnII and the 5 overhangs complemented with biotinylated nucleotides after which the blunt ends are ligated. Following ligation, crosslinks were reversed, and DNA separated from protein and any biotin not associated with internally ligated fragments. Purified DNA was sheared to 350 bp, and libraries constructed using an NEB Next enzyme kit followed by streptavidin bead purification and PCR enrichment before Illumina HiSeq X sequencing.