title: GSM4876772: x2; Mus musculus; RNA-Seq
library_construction_protocol: E18 dorsal forebrains were dissected from four brains per sex per condition (16 total samples from 8 litters). Tissues were treated with RNAlater and flash frozen on dry ice. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany). mRNA-seq library preparation (TruSeq® Stranded mRNA Library Prep, Illumina, Madison, WI) was tested using the DNA 1000 kit (Agilent) and 2100 Bioanalyzer Tapestation. 150 bp paired-end reactions across two lanes were used to sequence libraries (HiSeq 4000, Illumina).