title: chloroplast genome sequencing of leaf:Tulipa patens
design: DNA end repair, 3' end plus A, connection sequencing linker, agarose gel electrophoresis to recover the fragment of interest, PCR amplification of the fragment of interest, and finally build a sequencing library. The established library is quality inspected before sequencing, and after passing the quality inspection, the Illumina Novaseq TM platform is used for sequencing