title: GSM5227031: CAD2 RNAseq; Homo sapiens; ncRNA-Seq
library_construction_protocol: Peripheral blood samples (4-6 mL) were collected from CAD patients and healthy subjects using EDTA vacuum anticoagulant blood vessels. After a 30-min incubation at 4°C, the blood samples were centrifuged at 1,500 rpm for 10 min. Then, the plasma was recentrifuged at 12,000 rpm for 10 min to remove debris. Plasma RNA was extracted by TRIzolTM LS Reagent and total RNA (1 μg) was used to lncRNA-Seq high-throughput sequencing. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA,  USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).10 pM libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on Illumina HiSeq Sequencer according to the manufacturer's instructions.