title: Comparison of  Methanogens diversity between cattle and Buffaloes fed on similar diet and  kept in same environmental conditions
design: Amplicon libraries were prepared using the Nextera XT Index  Kit (Illumna Inc, USA). Archaea  specific primers (forward: ACGGGGYGCAGCAGGCGCGA; reverse: GGACTACVSGGTATCTAAT) were used for the  amplification of 16s rDNA. Amplicon  libraries were purified by AMPureXP beads and quantified using  Qubit fluorometer. The amplified  libraries were analyzed on 4200 Tape Station  system (Agilent Technologies) using D1000 Screen Tape.