title: mentagenome of 91 to 100% drupelets showed ash black disease syptoms
design: total DNA was extracted from each sample using the DNA kit (OMEGA, Bio-Tek, USA) following the manufacturers instructions. For each sample, the ITS region of fungal 16S rDNA was amplified using a primer set: ITS1 (5-TCCGTAGGTGAACCTGCGG-3), ITS2 (5-TCCTCCGCTTATTGATATGC-3) (Zhang, Huang and Chen 2015). Then PCR products were visualized on a 2.0% agarose gel and purified using AxyPrep DNA Gel Extraction Kit (AXYGEN) according to the manufacturers instructions. Prior to sequencing, the DNA concentration of each PCR product was determined using QuantiFluor -ST (Promega, USA) and quality was analyzed on an Agilent 2100 bioanalyzer (Agilent, USA). Purified amplicons were pooled in equimolar and paired-end sequenced (2  300) on an Illumina MiSeq platform (Illumina, San Diego,USA) according to the standard protocols by MajorbioBio-Pharm Technology Co. Ltd. (Shanghai, China)