title: IsoSeq of Dipus sagitta: adult female
design: For long-read RNA sequencing (Iso-Seq), total RNA was pooled from 6 tissues (heart, kidney, liver, lung, muscle, and spleen) collected from DS. A total amount of 5 g RNA that sample was used as input material for the RNA sample preparation. First strand cDNA synthesis employed the Clontech SMARTer PCR cDNA Synthesis Kit. The CDS Primer IIA was first annealed to the polyA tail of transcripts, followed by first-strand synthesis with SMARTScribeTM reverse Transcriptase. The first-strand product was diluted with Elution Buffer (EB) to an appropriate volume and subsequently used for large- scale PCR. Once double-stranded cDNA was prepared, the PacBio Template Prep Kit was used to generate SMRTbellTM libraries. The SMRTbell templates are then sequenced on the PacBio Sequel II platform.