title: miRNA of exosomes derived from allergic rhinitis mice
design: Total RNA samples were extracted using agarose electrophoresis and Nanodrop (ND-1000) quality inspection. RNA quantification, library construction, library quality determination with Agilent 2100 Bioanalyzer, sequencing library of mixed different samples, denatured into single strand DNA by 0.1M NaOH. 50 cycles of sequencing were performed on Illumina NextSeq 500 sequencer according to supplier instructions. The library was constructed using NEB Multiplex Small RNA Library Prep Set for Illumia. Use TruSeq Rapid SR Cluster Kit (#GD-402-4001, Illumina) for sequencing