title: GSM6052187: 134_NoStim_CD4Me; Homo sapiens; RNA-Seq
library_construction_protocol: Full-length cDNA was prepared from 500-1000 cells using half-volume SMART-SeqV4 reactions (Clontech, Mountain View, CA). Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina-compatible barcoded libraries. Libraries were pooled and quantified using a Qubit® Fluorometer (Life Technologies). Sequencing of pooled libraries was carried out on a HiSeq 2500 sequencer (Illumina) with paired-end 53-base reads and a target depth of 5 million reads per sample.