title: transcriptome analysis of  hemolymph in crab
design: Eighteen individuals were collected from the tanks every 6 h over 24 h (at 6:00, 12:00, 18:00, and 24:00). The gut tissue was extracted immediately under natural light. The hemolymph samples from the 20 crabs were placed separately in five 2 mL sterile centrifuge tubes, respectively, and were stored at 80 C until RNA extraction.