title: ITS2 rRNA of fecal: human
design: DNA was extracted from 200mg fecal using the QIAamp DNA stool mini kit. The ITS2 regions of the fungal 18S-26S ribosomal DNA cistron were amplified with ITS-V2-F (5-GTGARTCATCGAATCTTT-3) and ITS-V2-R (5-GATATGCTTAAGTTCAGCGGGT-56) primers.