title: GSM6098608: HeLa, sgG2E3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol extraction kit from TAKARA (D9108A). 2 μg of total RNAs of G2E3 KO , sgG2E3 control HeLa cells and SUV39H2 KO , sgSUV39H2 control HeLa cells were sent to Novogene Corporation for RNA-seq.Same amount of spike-in yeast RNA was added to each RNA sample for RNA-seq normalization. RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) . RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.