title: RNA-Seq of D. melanogaster: S2 Cells
design: RNA was extracted in triplicate from S2 cells originating from the same populations used for ChIP-Seq experiments, using Qiagen RNEasy plus extraction kit, which includes additional elimination of gDNA from samples. All RNA samples had RQN>7.0, as determined by Bioanalyzer instrument (Agilent). cDNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760G), using Ampure XP beads for cleanup. All S2 cell cDNA and ChIP libraries (22 in total) were pooled together and sequenced on a single lane of Illumina NextSeq (single-end, 75 bp).