title: vesicle
design: Small RNA sequencing was carried out using Hiseq (BGI) as follows. Briefly, the small RNA fragments in a size of 18 to 30 bp were isolated and purified using a PAGE gel. Purified small RNA was ligated with 3 and 5 adaptors, and then RT-PCR enrichment was performed by ABI StepOnePlus Real-Time PCR System. The PCR products were purified using 10% PAGE and used for deep sequencing.