title: GSM4725894: samp_9_1W; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was extracted from individual brains using the mirVana miRNA isolation Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA).  DNase treatment was performed on RNA to remove remaining genomic DNA using the DNA-free Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). Total RNA was lyophilised in RNAStable (Biomatrica, San Diego, CA, USA) and sent to Novogene (HK) for polyA construction of sequencing libraries. PE150 PolyA+ library preparaton was performed by Novogene using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB)