title: Nanopore Sequence of metagenome of Pampa river
design: A total of 100 ng DNA from each sample was end-repaired,   cleaned   up   with   1X   AMPure   beads.  Barcode  adapter  ligation  (BCA)  was performed  with  NEB  blunt/  TA  ligase  and  cleaned  with  1X  AMPure  beads.  Qubit  quantified adapter  ligated  DNA  samples  were  barcoded  using  PCR  reaction and  cleaned  up  with  1.6x  AMPurebeads.Sequencing  was  performed  on  GridION  X5 using  SpotON  flow  cell  R9.4  (FLO-MIN106)  in  a  48 hrs  sequencing protocol.