title: Bulk RNA Seq of PostPregnancy Mammary Epithelial Cell Derived Organoids with Essential Media Trimmed Raw Sequences
design: MEC derived pre and post-pregnancy organoids (n= 2-3 wells per technical replicate) were collected, dissociated and resuspended in Trizol for RNA extraction. Double stranded cDNA synthesis and Illumina libraries were prepared utilizing the Ovation RNA-seq system (V2). RNA-seq libraries were prepared utilizing the Ovation ultralow DR multiplex system. Each library (n=2 per experimental condition) was barcoded with Illumina True-seq adaptors to allow sample multiplexing, followed by sequencing on an Illumina NextSeq500, 76bp single-end run.