title: GSM5735845: Patient 5 primary; Homo sapiens; RNA-Seq
library_construction_protocol: Ten AGCT FFPE tissue sections of 5 µm thickness were mounted on objective slides, and areas with >80% AGCT cellularity based on hematoxylin and eosin slide review were scraped off into a microcentrifuge tube.Total RNA was extracted from the FFPE tissue using Maxwell RSC RNA FFPE kit Single-indexed mRNA libraries were prepared from 100 ng of RNA with QuantSeq 3' mRNA-Seq Library Prep Kit FWD