title: GSM5620842: uninfected lung-Saline rep4; Mus musculus; RNA-Seq
library_construction_protocol: lung lobes were collected 24 hrs after the last intranasal administration of Saline or LNA ASOs and tissues were lysed in DNA/RNA Shield (Zymo Research) and total RNA was extracted and cleaned by using RNeasy kit (Qiagen) according to the manufacturer's protocol. cDNA libraries were constructed from 500 ng of total RNA from lung tissues of mice according to the manufacturer's protocol of Stranded mRNA-seq kit (KAPA). Briefly, mRNA was captured and fragmentized to 100~300 bp. library construction was performed undergoing end repair, A tailing, ligation of  unique dual-indexed adapters (KAPA) and amplification of 10 cycles to incorporate unique dual index sequences.