title: GSM4775704: SLX18986_Adh5_mouse; Mus musculus; RNA-Seq
library_construction_protocol: The femurs, tibiae, iliac crest, humeri, and vertebrae of 6-16 weeks old mice were crushed, washed with 10 ml of PBS supplemented with 2 % heat-inactivated FBS, and strained through 70-μm meshes. Cell suspension was depleted of red blood cells by ammonium chloride lysis (STEMCELL Technologies), and stained with the lineage depletion kit (19816A, STEMCELL Technologies) following the manufacturer's instructions. Cells were washed in PBS with 0.04% BSA and re-suspended before loading 10,000 cells onto each channel of the Chromium 10x Genomics platform to capture single cells in droplets. Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell 3ʹ Reagents Kits User Guide: CG00052. Quantification of cDNA was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA tape-station (Agilent. 5067-5584). Quantification of library construction was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA tape-station (Agilent. 5067-5584).