title: GBS of barley
design: Digestion with PstI and MspI for 100 ng of the qualified DNA of each accession.The pre-treated adapters were then ligated to sticky ends by adding 30 L of a master mix solution that contained 4 U T4 ligase to each well.Fragments with sizes of 300-500 bp were harvested for gel extraction and the quantified samples were being ready for sequencing on Illumina X10 .