title: GSM6099142: PSCCreMRpos_R_E10_S290; Mus musculus; RNA-Seq
library_construction_protocol: Retinas were dissected in oxygenated AMES, digested in papain, and dissociated to single cell suspensions using manual trituration in ovomucoid solution. Single cell suspensions were incubated with 0.5ul of anti-CD90,  (Thermo Fisher Scientific) for 10 million cells per 100µl. Cells were sorted using a MoFlo Astrios. Cellular debris, doublets, and dead cells were excluded, and RGCs were collected based on high CD90 and GFP and mCherry (for MR+) or without mCherry (MR-)co-expression. Cells were collected directly into 96well plates, 1 cell/well and frozen at -80C. Cells were processed following published SS2 protocols, using pre-beads clean up and purification and Nextera XT kit from Illumina. Libraries were sequenced on NovaSeq-SP