title: GSM6051365: Brown adipocytes, ChIP-Tra; Mus musculus; ChIP-Seq
library_construction_protocol: Freshly dissected small pieces of brown adipose tissue were crosslinked during 25min at room temperature under agitation in the crosslink solution (250mM disuccinimidyl glutarate, 50mM HEPES, 100mM NaCl, 1mM EDTA, 0.5mM EGTA). Then, 1% formaldehyde was added for 20 further minutes followed by 50mM glycine addition. The tissue was rinsed several times with PBS. Chromatin immunoprecipitation was then performed as previously described (Chatonnet et al., 2013). Sequencing libraries were prepared from the immunoprecipitated fraction and the input fraction as control, using the Accel-NGS 2S Plus DNA library kits with single indexing (Swift Biosciences).