title: DNA-seq of Castanea mollissima Blume: leaves
design: 1.The addition of formaldehyde solidifies proteins in the genome that are involved in chromatin interaction; 2.The genome is cleaved with restriction enzymes; 3.The resulting fragment has a flat or sticky end, which is then smoothed and repaired with biotin; 4.T4 DNA ligase was used to link the interacting fragments to form loops.The proteins that bind the DNA fragments together are digested to produce cross-linked fragments; 5.Ultrasonic interruption; 6.Magnetic beads were used to capture biotin, make a library, and sequence it on the machine.