title: GSM4998461: Pulp5; Homo sapiens; RNA-Seq
library_construction_protocol: PULP: Teeth (third molars) were collected immediately after extraction and preserved in sterile NaCl 0.9%, on ice for the time needed to transfer them from the clinic to the processing laboratory (< 10 minutes).  Periodontium was carefully removed (see below). The cleansed tooth was then carefully wiped with 70% ethanol. The tooth was then cracked with a press, and carefully opened with forceps. The dental pulp was then removed from the tooth with a separate set of instruments, placed in a Petri dish filled with cold HBSS and minced into small pieces (< 2 mm diameter). Dental pulp pieces were transferred in falcon tubes filled with HBSS, centrifuged at 4°C, 300g, for 10 minutes. Tissues were digested in 10 mL Collagenase P 5 U/mL (11 213 873 001, Sigma Aldrich, Buchs, Switzerland) for 40 minutes at 37°C. After digestion, samples were disaggregated by pipetting, filtered through a 70 um cell strainer, and resuspended in HBSS + 0.002% Bovine Serum Albumin (BSA; 0163.2, Roth AG, Arlesheim, Switzerland). library preparation was performed according to the manufacturer's indications (single cell 3' v2 or v3 protocol)