title: Microviruses in Heloderma suspectum fecal samples
design: 5g of fecal sample was homogenized in 20ml SM buffer (0.1M NaCl, 50mM Tris HCl [pH 7.4]) and centrifuged at 10000g for 10 min. The resulting supernatant was filtered sequentially through a 0.45 m and 0.2 m syringe filter. 200 l of the filtrate was used to extract viral DNA using the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). Circular viral DNA was amplified using rolling circle amplification (RCA) with Illustra TempliPhi 100 Amplification Kit (GE Healthcare, USA). The RCA products were used to prepare a 2x150bp paired-end library and sequenced on a NextSeq500.