title: hMeDIP-Seq of Homo sapiens : healthy volunteers  CD4+T cells
design: hMeDIP-seq was performed on Illumina HiSeq X ten (Illumina Inc.; San Diego, USA) with the hMeDIP Kit (Active Motif, Carlsbad, USA). MACS2 (version: 2.1.2) software was used to call 5hmC-occupied peaks representing enriched hydroxymethylation sites. Occupancy analysis and normalization of the peaks were conducted by using the R package DiffBind (version: 2.10.0). DiffBind used edgeR to analyze differential hydroxymethylated peaks/regions (DhMR). A P-value and false discovery rate (FDR) were assigned to each candidate peak indicating the significance of their being differentially hydroxymethylated. Only peaks with FDR 0.05 were identified as significantly differential peaks.