title: Commensal Escherichia coli serotype O2:H6 (DPI:42) (Group:Str-Amp) (ID:PG20180121)
design: Representative colonies isolated from media plates for enumerating transconjugants were subjected to whole genome sequencing using an Illumina MiSeq system and/or an Oxford Nanopore MinION sequencer (Oxford Nanopore, Cambridge, MA, USA) at the National Microbiology Laboratory (Guelph, Ontario, Canada). Genomic DNA was isolated from the bacteria using the automated Qiagen EZ1 DNA tissue kit, according to manufacturers instructions, except 180 L of G2 buffer was used 177 with 10 L of proteinase K and 10 L of lysozyme (10 mg/mL; Sigma-Aldrich, Gillingham, UK).