title: GSM6051591: C2C12, Lenti-dCas9-KRAB, contr_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using using HiPure Total RNA Mini Kit (Magen). 2 ug of total RNA was used for the construction of sequencing libraries. Total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse.