title: 16S rRNA sequencing (of the V1-V2 region) of Indian Ocean bacterial communities
design: Amplification of the V1-V2 region of the 16S rRNA gene using the primers 8F (5- AGA GTT TGA TCC TGG CTC AG-3) and 338R (5- GC TGC CTC CCG TAG GAG T-3). A second PCR was conducted to add dual indexes - the forward primer overhang adapter (5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG-3) and reverse overhang adapter (GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G) were both specific to Illumina.