title: Illumina HiSeq 4000 paired end sequencing; Whole Transcriptome Sequencing of Cervical Adenocarcinoma
design: Whole Transcriptome Sequencing of Cervical Adenocarcinoma
library_construction_protocol: The normal tissues and tumor tissues were collected from ACTREC-TMC Total RNA was extracted from tumor tissue and available normal tissue sample using TRIzol reagent Library preparation was performed using SENSE Total RNA-Seq Library Prep Kit from Lexogen (Lexogen Inc). The ribosomal RNA (rRNA) was depleted from Total RNA using RiboCop rRNA Depletion Kit V1.2 (Lexogen Inc). RNA samples are then subjected to reverse transcription and ligation in a single tube employing starter-stopper heterodimer primers. Then the cDNA was enriched for sequencing.