title: GSM6051387: 19128X21 Female, Adrenal Gland, 12-week, SF1-Cre+;Znrf3Fx/Fx, biol rep 1; Mus musculus; RNA-Seq
library_construction_protocol: RLT lysis buffer (Qiagen, 74104) containing 1% b-mercaptoethanol was added to thawed adrenal tissue in high impact zirconium 1.5mm bead tubes (Benchmark Scientific, D1032-15). Samples were homogenized for 30 seconds using the Beadbug™ 3 (Benchmark Scientific, D1030), returned to ice, and homogenized for an additional 30 seconds. Total RNA isolation was completed using the RNeasy® Kit (Qiagen, 74104) according to manufacturer guidelines. DNases were removed using the RNase-Free DNase Set (Qiagen, 79254) and subsequently purified using the RNeasy MinElute Cleanup Kit (Qiagen, 74204) according to manufacturer guidelines NEBNext Ultra II Directional RNA Library Prep with poly(A)mRNA Isolation