title: GSM4878258: G1 phase - DMSO - Replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from two replicates of the synchronized HeLa cells using the RNeasy Plus Mini Kit (QIAGEN) as per the manufacturer's protocol. While isolating total RNA for RNA-seq analysis, on-column digestion of DNA was additionally performed using RNase-Free DNase Set (QIAGEN) as a precautionary measure and later subjected to the mRNA-seq library preparation.   RNA libraries were prepared for sequencing using standard Illumina protocols