title: Metatrancriptomic of oral cavity: supragingival dental biofilm
design: True Seq Sample Preparation Guide, Low Sample (LS) Protocol Illumina (Illumina, Inc., San Diego, CA) was used for genomic library preparations and Agilent Technologies 2200 TapeStation was used for genomic library quality validation. The double strand DNA was quantified with Quant-iTTM PicoGreen dsDNA Kit (Turner BioSystems, Inc., CA) before sequencing at the Illumina HiSeq3000 (Illumina Inc.) (2x150bp).