title: Illumina HiSeq 2500 paired end sequencing; Whole exome sequencing of EGFR-TKI naive and EGFR-TKI resistant lung adenocarcinoma samples
design: Whole exome sequencing of EGFR-TKI naive and EGFR-TKI resistant lung adenocarcinoma samples
library_construction_protocol: 48 DNA samples corresponding to 16 EGFR mutant lung adenocarcinoma patients were collected retrospectively from tumor tissue repository at Tata Memorial Hospital, Mumbai. The sample collection and study protocols were approved by Institutional Review Board at TMC-ACTREC.  Genomic DNA extraction from FFPE tumor blocks (both sensitive and resistant) was performed according to standard protocol of QIAmp DNA FFPE Tissue Kit (Catalog No 56404). Genomic DNA extraction from blood was performed according to standard protocol of (). DNA concentration and quality was assessed using Qubit 3.0 fluorometer and Tape Station respectively. Whole exome sequencing was performed on 33 DNA samples using a minimum of 100ng input DNA (based on Qubit quantification) for library preparation. Exome capture was performed using the TruSeq Exome Library Prep Kit. Sequencing was performed on Illumina platform according to standard protocol using 150bp paired end reads