title: DNA-seq of Castanea mollissima Blume: leaves
design: 1.The DNA molecules to be sequenced were broken into 200-500 BP fragments by ultrasonic wave. 2. The ends of the broken genome were flattened. 3. The 3 'ends of the flattened fragments were added with a and 5' ends for phosphorylation. 4. The repaired fragments were spliced. 5. The spliced DNA fragments were amplified by PCR. 6. The amplified library was purified by magnetic beads