title: RNA-Seq of flower (and stem, leaf) in Magnolia polytepala
design: The equal amount of total RNA from each type of plant samples was mixed for the follow-up operations. The mRNAs containing poly(A) were enriched by Oligo (dT), and then underwent reverse transcription to generate full-length first strand cDNA using UMI base PCR cDNA Synthesis Kit (BGI, Wuhan, China). Subsequently, PCR amplification was applied to second strand cDNA synthesis. After large-scale amplification, the double-stranded DNA was subjected to optional size selection for combined SMRTbell library construction, and further PacBio Iso-Seq.