title: mosquito PfSPZ replicate 1
design: total RNA from mosquito produced or in vitro produced PfSPZ was extracted using the miRNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including on-column DNase digestion. Total RNA was poly(A)-enriched using the Dynabeads mRNA Purification Kit (Life Technologies) and subjected to strand-specific RNA-seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina)