title: S. callimorphus genomic short reads
design: Scolanthus callimorphus animals were collected at the le Callot, Carantec, France. After transport, they were kept in seawater at 20 C and fed freshly hatched Artemia salina weekly or biweekly. High molecular weight DNA from a single Scolanthus callimporphus adult animal was extracted using a modified Urea-based DNA extraction protocol. A whole animal was flash frozen and ground with mortar and pestle. While frozen, drops of buffer UEB1 (7M Urea, 312.5 mM NaCl, 50 mM Tris-HCl pH 8, 20 mM EDTA pH 8, 1 % w:v N-Lauroylsarcosine sodium salt) were added and crushed with the tissue. Tissue was incubated in a final volume of 10 mL UEB1 at RT for 10 minutes. Three rounds of phenol-chloroform extraction were performed followed by DNA precipitation by addition of 0.7 volume isopropanol. The pellet was transferred to a fresh tube and washed twice in 70 % EtOH and twice more in 100 % EtOH, dried, and resuspended in TE buffer. An aliquot of the same sample was used to prepare a library using the NEBNext Ultra II DNA Library Prep Kit for Illumina.