title: GSM6052263: ELAC2A537T TRAMP +/- 30weeks miRNA rep 1; Mus musculus; miRNA-Seq
library_construction_protocol: Individual prostate lobes were dissected as described previously by Zingiryan et al., 2017. Briefly, an OPMI 6-SFC Universal Microscope (Zeiss) was used to remove each prostate lobe to isolate high quality and pure tissue. Upon removal of the urogenital system from the sacrificed animal, all tissue was completely submerged in PBS. The seminal vesicles, bladder, urethra and surrounding connective or adipose tissue were removed and then the individual ventral, lateral, anterior and dorsal lobes were dissected and frozen in liquid nitrogen. Sequencing was performed using the Illumina NovaSeq platform, prepared with the Illumina TruSeq Small RNA-Seq library prep kit according to manufacturers instructions.