title: mitochondrial genome of Anatrichus pygmaeusadult muscle tissue
design: The total DNA was extracted from the adults muscle tissue using DNeasy Blood & Tissue Kit (Qiagen, Germany). All quantified DNA extracts were included in a single pool at equimolar concentration, aiming for 50 ng/uL of dsDNA per sample, resulting in a DNA pool of approximately 5ug. The library was sequenced on an Illumina NovaSeq 6000 by Novogene. Raw read data were filtered and trimmed in Trimmomatic v0.30