title: GSM5348425: Lac_C; Bifidobacterium pseudocatenulatum; RNA-Seq
library_construction_protocol: Bacterial cells were immediately pelleted and resuspended in 1ml of RNAprotect Bacteria Reagent. Cells were pre-lysed lysozyme and mutanolysin. Then, Total RNA is extracted using the RNeasy mini kit (Qiagen Inc, Valencia). Total RNA samples were DNase treated twice. Ribosomal RNA depletion was carried using RiboMinus Transcriptome Isolation Kit, bacteria (Thermo Fisher, Waltham, MA) on 1 μg of total RNA. Barcode indexed RNA-seq libraries were generated from 20 ng ribo-depleted RNA using the Kapa Hyper Stranded RNA-seq kit (KapaBiosystems, Cape Town, South Africa).