title: Ventral diencephalon_at_E10.5_rep3
design: We microdissected the dosal diencephalon and ventral diencephalon from three control mice at E10.5 and isolated the total RNAs with Trizol reagent (Ambion). Reverse transcription and template switching were performed with oligo(dT) guided primers and SuperScript II Reverse Transcriptase (Invitrogen, #18064-022) according to the Smart-seq2 protocol.