title: GSM5840474: EB-Flag-Dox; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were dissociated using versene, washed once in cold PBS and counted. Approximately 6x10^6 cells were fixed and chromatin was PIXUL sonicated as described above. H3K27me3 (Active Motif Ab) Matrix-ChIP'ed DNA was  purified using phenol-chloroform extraction and ChIP-seq libraries were prepared (Next Gen DNA Library Kit, Active Motif, Cat# 53216, 53264). Libraries were sequenced on NextSeq2000 in single end run and approximately 45 million raw reads were obtained for each sample.