title: RNA-Seq of Koroneiki:stage_3_fruit_rep2
design: Total RNA was extracted from fresh tissue material for 14 tissue types of the olive variety "Koroneiki" in three biological replicates, using the Monarch Total RNA Miniprep Kit (NEB, Ipswich, MA, USA). The RNA libraries were prepared using the NeBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and library indexing was performed using the NEBNext Multiplex Oligos for Illumina kit (NEB, Ipswich, MA, USA), according to the manufacturers instructions. RNA library quantity and quality were assessed using the Qubit dsDNA HS Assay Kit (Invitrogen, Oregon, USA), the 2100 Bioanalyzer (Agilent Technology Inc., CA, USA) using the Agilent High sensitivity DNA kit and the NEBnext library quant for Illumina kit ((NEB, Ipswich, MA, USA). Sequencing was performed in the NextSeq500 platform (Illumina, San Diego, CA, USA), using the NextSeq 500/550 High Output Kit v2.5 (300 Cycles), paired-end, 2x150 bp.