title: RNA-seq of mus musculus: Ly6C- MDP in bone marrow
design: For Bulk RNA-seq, 3,000-20,000 cells of indicated cell populations were sorted directly into 500 L TRIzol reagent (Invitrogen) on an Aria III flow cytometer. RNA extraction was conducted according to manufacturers instructions, with GlycoBlue added for RNA coprecipitation. Reverse transcription and 12-cycle cDNA amplification were performed with a SMART-Seq HT Kit (TaKaRa). Sequencing libraries were prepared using the TruePrep library preparation kit (Vazyme). Pooled libraries were run on an Illumina X10 platform with ~12 million paired reads for each sample.