title: GBS data of Cochliomyia hominivorax larvae
design: Total DNA was double-digested with PstIMspI restriction enzymes. Barcoded adapters were ligated on the rare cut site (i.e., PstI cut site) for each individual separately, and the common adapter was subsequently ligated on the common cut site (i.e., MspI cut site) for all individuals. The pool was amplified by multiplexed PCRs using standard forward primer A and modified reverse primer C with a high-fidelity DNA polymerase. PCR products were purified with magnetic beads and the DNA amount was estimated with NanoDrop. Library quality was accessed with the evaluation of fragment sizes and the presence of adapter dimers in a BioAnalyzer. The library was sequenced using 150-bp single-end reads (Illumina NextSeq 500).