title: GSM5393646: control sample3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted by Trizol A total amount of 3 ?g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo- attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLVreverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently