title: Amplicon sequencing of microbial community during full-scale activated sludge transplantation
design: DNA extraction, sample preparation including amplification of V1-3 region of 16S rRNA gene using the 27F (AGAGTTTGATCCTGGCTCAG) (Lane, 1991) and 534R (ATTACCGCGGCTGCTGG) (Muyzer et al., 1993) primers, and amplicon sequencing were conducted as described in MiDAS protocols (https://www.midasfieldguide.org/guide/protocols)