title: sncRNA-seq of drosophila melanogaster: whole embryo
design: libraries prepared using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) according to manufacturers protocol with some changes. We downscaled all samples to half volume and added 2SrRNA block oligo (5-TAC AAC CCT CAA CCA TAT GTA GTC CAA GCA-SpcC3 3; 10 M) . We selected bands of sizes 140-170 bp (including adaptors and priemrs).