title: Rattus_norvegicus_lociPCR_male_testis_young_15
design: DNA samples were standardized to 10 ng/ul. We amplified the autosomal and Y-linked loci in the same PCR reaction using the Phusion Flash High Fidelity from Thermo Fisher Scientific (Cat. No. F548L). PCR products were purified using Agencourt AMPure XP (Cat. No. A63882). PCR products were multiplexed and sequenced in a NextSeq 500 Illumina machine (single-end, 75 nucleotides long) at UNAM. The quality of the reads was verified using FastQC, and the remaining adaptors and low-quality bases were removed with Trimmomatic (v.036. Reads were aligned using bowtie2 (v. 2.3.4.1) against the genome sequence of the rat from ENSEMBL database. We then extracted the frequency of reads that mapped uniquely to the expected loci.