title: Eukaryotic transcriptome of intestinal mucosa
design: 1) Use magnetic beads with Oligo (dT) to conduct AT base pairing with polyA to separate mRNA from total RNA; 2) Add fragmentation buffer to randomly fragment mRNA, and separate small fragments of about 300bp by magnetic bead screening ; 3) Under the action of reverse transcriptase, random hexamers are added to reversely synthesize one-strand cDNA using mRNA as a template, followed by two-strand synthesis to form a stable double-strand structure; 4) Double-strand The cDNA structure has sticky ends, and End Repair Mix was added to make up the blunt ends, and then an "A" base was added to the 3' end for connecting a Y-shaped linker.