title: RNA-Seq Illumina Novaseq of Vanilla planifolia: ovary
design: mRNA were selected using poly-T beads. Then, RNA were fragmented to generate double stranded cDNA and adaptators were ligated to be sequenced. 11 cycles of PCR were applied to amplify libraries. Library quality was assessed using a Fragment Analyser and libraries were quantified by QPCR using the Kapa Library Quantification Kit. RNA-seq experiments have been performed on an Illumina NovaSeq using a paired-end read length of 2x150 pb with the Illumina NovaSeq sequencing kits.