title: 16S rRNA of Rhizophora mangle: fruits
design: We used the following forward/reverse bacterial primer pair: S-D-Bact-0341-b-S-17/S-D-Bact-0785-a-A-21. 16S rRNA gene sequencing amplicon libraries were prepared using a two-step PCR as described in the Illumina 16S Library Preparation guide with the following modifications: intermediate and final amplicons were examined by agarose gel electrophoresis, quantified by Qubit dsDNA HS Assay (Invitrogen: Carlsbad, CA), and pooled at equimolar concentrations. The library pool was analyzed using Agilent's TapeStation D1000 High Sensitivity Assay (Agilent: Santa Clara, CA) and quantified using KAPA qPCR for Illumina libraries. Libraries were sequenced on an Illumina MiSeq (San Diego, CA) using MiSeq Reagent Kit v3 (2x300 cycles)