title: ChIP-seq of Rics:shoot meristem enrichment tissue
design: ChIP DNA libraries were prepared using the TruSeq DNA Sample Preparation Kit v2 (Illumina). Briefly, 10 ng precipitated DNA was end-repaired, single A nucleotide was added to the 3 end of the blunt ends of the fragments followed by adapter ligation, library fragments of 250 to 300 bp (insert plus adapter sequences) were isolated from an agarose gel, and PCR was performed with a PCR primer cocktail to enrich DNA fragments. After purification, the DNA fragments were sequenced with the Illumina HiSeq-2000.