title: GSM5514494: w, replicate 3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Wing imaginal discs from third instar female larvae were dissected, and total RNAs were extracted using the RNA easy mini kit (Qiagen).  Samples wc_1, wc_2, corto[L1]/corto[420]_1, corto[L1]/corto[420]_2 were treated and sequenced  as described (Coléno-Costes et al., 2012).  For the other samples, the protocole was the same except that 150 discs were homogenized by pipetting and used for each extraction. For samples wc_1, wc_2, corto[L1]/corto[420]_1, corto[L1]/corto[420]_2, libraries were prepared as described (Coléno-Costes et al., 2012).  For the other samples, libraries were prepared using the strand specific RNA-Seq library preparation TruSeq Stranded mRNA kit (Illumina) (1 µg de mRNA per sample). For samples wc_1, wc_2, corto[L1]/corto[420]_1, corto[L1]/corto[420]_2, see Coléno-Costes et al., 2012.  The other samples were multiplexed by 15. Each genotyped was deposited on 4 flowcell lanes.