title: Single-cell RNA-seq of human ovarian tumor: Sample 16030X3_HJTWLDMXX
design: De-identified HGSOC samples were processed after cryopreservation in liquid nitrogen where tissue chunks were stored in RPMI media with 10% fetal bovine serum and 10% DMSO. Samples were thawed and dissociated to single cells using the Miltenyi Human Tumor Dissociation Kit and the GentleMACS dissociator. Samples were incubated on the GentleMACS at 37°C for 1 hour with the setting of 1,865 rounds per run. A 70 µm MACS smart strainer was used to deplete cell doublets before loading onto the 10x Genomics Chromium Controller. Library preparation was performed using the 10x Genomics 3' Gene Expression Library Prep v3 and libraries were sequenced on an Illumina NovaSeq instrument.