title: GSM5620877: Single cell RNA-seq of Mouse dura cells; Mus musculus; RNA-Seq
library_construction_protocol: To exclude intravascular leukocytes, FITC-labeled CD45 antibody was intravenously injected (CD45 IV) 5 min before intracardiac perfusion. We used mechanical isolation to get dura leukocytes lest enzymatic digestion makes hematopoietic progenitors undetectable. Dura cells from four 8 ~ 12-week-old mice were combined together. All leukocytes were stained with PE-labeled CD45 antibody and DAPI was used to exclude dead cells. CD45 IV−DAPI− CD45-PE+ singlets were then sorted Libraries were prepared using the Chromium Single cell 3' Reagent Kits v3.1 (CG000204): Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (PN-1000128), Chromium Single Cell 3' Chip Kit v2 (PN-1000127) and Single Index Kit Set A (PN-1000213). Libraries were sequenced on an Illumina NovaSeq 6000 using paired-end sequencing with approximately >90% sequencing saturation.