title: RNA-Seq of glioma cell
design: Cells were harvested and total RNA was extracted by Trizol reagent. Beads with oligo(dT) were used to isolate poly(A) containing mRNA  after total RNA was collected before the cDNA library construction via M-MuLV system. The cDNA was selected by AMPure XP beads for amplification and  sequencing using Illumina NovaSeq 6000 platform. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using HISAT2 (version 2.1.0).