title: COI Seq of Antrostomus vociferus: feces
design: An initial PCR was performed to amplify a 180 bp region of the COI gene using the primer pair LCO11490 (5-GGTCAACAAATCATAAAGATATTGG-3) and CO1-CFMRa (5-GGWACTAATCAATTTCCAAATCC-3) (Jusino et al. 2019). A second PCR was then performed to add unique dual-indexes to each sample using the amplicons from the initial PCR as a template.