title: SI
design: Total RNA extracted using RNeasy Mini Kit (Qiagen) Total RNA was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Tech., Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agrose gel. g total RNA withg total RNA with RIN value above 7 was used for following library preparation. NGS library were constructed according to the manufacturers protocol (NEBNext UltraTM RNA Library Prep Kit for Illumina)