title: GSM5896604: S. glacialis, day 5, 1 h after the switch to dark phase, replicate B; Sphingomonas glacialis; RNA-Seq
library_construction_protocol: Cells were harvested by centrifugation. Pellets were resuspended in 1 ml PGTX extraction solution (Pinto et al., 2009) and immediately frozen in liquid nitrogen. RNA was extracted and processed as described earlier in  Kopejtka et al. (2020). Libraries for transcriptomics were generated according to Shishkin et al., 2015 including rRNA removal with the RiboZero Kit (Illumina Inc., San Diego, CA, US).