title: RNA m6A methylation of zebrafish
design: M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit (GenSeq).NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without immunoprecipitation and IP RNA samples after immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer (Agilent).High-throughput sequencing was performed using a 150 bp dual-terminal mode on Illumina NovaSeq 6000 sequencer.