title: GSM4819315: Cells treated with BI6727 and MK-0752, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using QIAGEN RNeasy Mini Kits. A total of 16 samples were submitted to the University of Wisconsin Biotechnology Center Gene Expression Center for quality control, library construction and further analysis. The purity and integrity of total RNA were assessed on the NanoDrop One Spectrophotometer and Agilent 2100 BioAnalyzer, respectively. Samples that met the TruSeq Stranded mRNA Sample Preparation Guide (Rev. E) input guidelines were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation kit. The quality and quantity of prepared libraries were assessed by analysis on the Agilent 2100 Bioanalyzer and Qubit fluorimeter. An equimolar pool of all libraries with 30 million targeted reads per sample was sequenced on the Illumina NovaSeq S4 flowcell with 2x150bp sequencing. Images were analyzed using the standard Illumina Pipeline (v1.8.2).