title: NGS of Phodopus roborovskii: adult female
design: To construct short-insert libraries (350 bp insert sizes) for Illumina sequencing, 5 g of DNA was sheared into fragments, end-repaired, A-tailed and ligated to Illumina paired-end adapters (Illumina, San Diego, USA). The ligated fragments were fractionated on agarose gel and amplified by PCR to produce short-insert libraries. Finally, the short-insert libraries were quantified and sequenced on the Illumina NovaSeq 6000 platform.