title: GSM5897060: Bisulfite-seq_Lens fiber cells_rep1; Gallus gallus; Bisulfite-Seq
library_construction_protocol: Lenses were isolated from White Leghorn embryonated chicken eggs (Charles River Laboratories, Storrs, CT) at embryonic developmental stage day 13 (E13). 25 lenses per biological replicate were microdissected into epithelial cells and fiber cells as previously described [217]. Microdissected epithelial and fiber cells from 25 lenses were pooled and stored in CryoStor CS10 cryopreservation media containing 10% DMSO (Stemcell, Vancouver, Canada) and stored at −80 °C as previously described [237]. Tissue in CryoStor media was thawed on ice, centrifuged at 800 x g for 5 mins and the CryStor removed. DNA was isolated using Qiagen Genomic-tip 20/G along with the Qiagen Genomic DNA buffer set according to the manufacturer's instructions. Bisulfite-seq was performed by Novogene (Sacramento CA) using an Illumina HiSeqTM2500/MiSeq platform using their standard protocols