title: RNA-Seq of Pasteurella multocida: NPTr cells
design: Sequencing libraries were generated using a NEBNext UltraTM 84 RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) following manufacturers recommendations. Library preparations were sequenced on an Illumina Novaseq platform at Novogene Co., Ltd. (Beijing, China), using the pair-end 150-bp sequencing protocol. After sequencing, raw data of fastq format were firstly processed through in-house perl scripts. In this step, clean data were obtained by removing reads containing adapter, reads containing ploy-N and low-quality reads from raw data. At the same time, Q20, Q30 and GC content of the clean data were calculated.