title: GSM4775399: control; Danio rerio; RNA-Seq
library_construction_protocol: After exposure, forty embryos collected from each group were transferred to 1 mL phosphate buffer saline (PBS) with 1% bovine serum albumin (BSA). After incubated in 0.5 mL Hank's balanced salt solution (HBSS) containing 0.6 U/mL of dispase (Yuanye Biotechnology, Shanghai, China) at 37 °C for 5 min, 100 µL of fetal bovine serum (FBS) was added to stop digestion. The cell suspension was filtered through a 40 µm filter and washed by 1% BSA-HBSS. The cells were finally transferred to 1 mL PBS in preparation for single-cell sequencing. The obtained cells were loaded on a GemCode Single Cell Instrument (10× Genomics, Pleasanton, USA) to generate single-cell gel beads in the emulsion. ScRNA-seq libraries were prepared using the GemCode Single Cell 3′ Gel Bead, Chip and Library Kits (10× Genomics, Pleasanton, USA).