title: GSM5579451: Eyecup Tmem135 FUN025/+ rep3; Mus musculus; RNA-Seq
library_construction_protocol: Posterior eyecups without the neural retina were collected and pooled from individual 2.5-month-old WT, Tmem135FUN025/+, and Tmem135FUN025/FUN025 mice of both genders between 11:00 and 1:00 PM. Samples were immediately flash frozen. Total RNA was extracted from eyecups with an RNeasy Plus Universal Mini kit (Qiagen, Hilden, Germany) following the Manufacturer's instructions. RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturer's instructions.