title: RNA seq of human coronary artery smooth muscle cells, treated with 10 uM 2-deoxyuridine, 24h
design: Total RNA of each sample was extracted using RNeasy Mini kit (Qiagen, Hilden, Germany), quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agrose gel. Library preparation and next generation sequencing were performed by Genewiz (Suzhou, China) using 1 g total RNA (RIN value above 7) for library preparation according to the manufacturers protocol (NEBNext Ultra RNA Library Prep Kit for Illumina) and sequenced on a NovaSeq 6000 instrument (Illumina, San Diego, CA, USA) to generate 2x150 paired-end, 31-55M reads per sample.