title: GSM5840147: CD8_naïve_0h_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Micro Kit (QIAGEN), or ZR-96 Quick-RNA kit (Zymo Research), according to the manufacturer's instructions. cDNAs were generated from isolated RNAs using SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Takara/Clontech). Barcoded libraries were generated by the Nextera XT DNA Library Preparation kit (Illumina) and sequenced with a 2x100 bp paired-end protocol on the HiSeq 4000 Sequencing System (Illumina).