title: RNAseq of myoblast parental cell line
design: Starting input material for the libraries construction was DNA free total RNA using the TruSeq RNA Sample Prep Kit v2 according to manufacturers protocol. In short, 0.5 g of total RNA was used for poly-A based mRNA enrichment selection followed by fragmentation by divalent cations resulting into fragments of 80-250nt, with the major peak at 130nt. Double stranded cDNA was end repaired, 3adenylated and the 3-T nucleotide of barcoded Illumina adapters was used for the adapter ligation. The ligation product was amplified with 12 cycles of PCR and quality controlled in Agilent DNA 7500 Bioanalyzer assay.