title: DNA-Seq of Impact crater: Dhala crater
design: 1) DNA Isolation (FastDNA Spin Kit MP Biomedicals, USA), (2) Quantity of DNA : Done by picogreen* method using Victor 3 fluorometry, (3) Assesing the condition of the DNA : Done by gel electrophoresis method, (4) Metagenome Amplicon Generation by qPCR, (5) Library Size Check by Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip, (5) Library Quantity Check by illumina library (qPCR according to the Illumina qPCR Quantification Protocol Guide) and GS FLX library (Roche's Rapid library standard Quantification solution and calculator)