title: 16S rRNA amplicon sequencing of Wistar rat gut microbiome fed with control diet supplemented with the polyphenol preparation and fructooligosaccharides
design: Total DNA was extracted from the caecal rat microbiota using a QIAamp Fast DNA Stool Mini Kit (Qiagen) following the manufacturers instructions. DNA was used to assess the microbiota profile by amplification of the V3-V4 region of the 16S rRNA gene using primers and protocols according to the 16S metagenomic sequencing library preparation instructions. The paired-end sequencing reaction (2  250bp) was performed using an Illumina MiSeq platform according to the manufacturers instructions.