title: GSM6102020: HeLa_total_RNA_ModSeq_Mg; Homo sapiens; OTHER
library_construction_protocol: Trizol cDNA was prepared from total RNA from each source using random nonamer primers and MarathonRT enzyme in the presence of either Mg or Mn as enzymatic cofactors; second-strand synthesis was carried out from each cDNA sample using NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module; library preparation was carried out from the resulting dsDNA sample using the Nextera XT DNA kit Illumina Nextera XT DNA