title: GSM5348513: TEG_medium_exposed control 1; Homo sapiens; RNA-Seq
library_construction_protocol: TEGs (>0,8x106 per condition) were cultured in normal medium without addition of PDOs (no target control), using 12-wells culture plates (Thermo Fischer) . Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000; ThermoFisher) for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at -80°C until further processing. All sorted plates were processed according to the CEL-Seq2 protocol with the total transcriptome amplification via poly-A RNA-capture, library preparation, and sequencing into Illumina sequencing libraries as previously described(Muraro et al., 2016). Paired-end sequencing (read1: 30 bp; read2: 120 bp) was used to sequence the prepared libraries using an Illumina NextSeq sequencer.