title: GSM5097971: fer-8-Root-Rep-1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: QIAGEN RNeasy Mini Kit RNA samples with a concentration higher than 300 ng/uL and RIN number high than 8 were used for library preparation. Construction of libraries and sequencing were performed at the Michael Smith Genome Sciences Center (http://www.bcgsc.ca/). Paired end 75 bp RNA-sequencing was performed using an Illuminia Hi-seq 2500 platform. The total RNA was DNase I treated and QC'ed using Agilent RNA Nano assay for quality and concentration.   Ribosomal RNAs (rRNA) are generally removed from total RNA either by polyA+ selection.