title: GSM6103020: Proneural glioblastoma stem cell line 0512 (biol rep 2); Homo sapiens; RNA-Seq
library_construction_protocol: RNA from PN and MES GSCs was extracted by using the RNeasy mini kit (Qiagen) according to manufacturer's protocol. The cDNA was synthesized starting from total RNA using SMART technology. After barcoding, the RNA libraries were pooled, denatured, and diluted to 2.4 pM final concentration. RNA sequencing was performed by using NextSeq (Illumina) set for 76 cycles in single end (SE)