title: GSM5098147: HTGTS_PC-9.hA3C_before rep3; Homo sapiens; OTHER
library_construction_protocol: Rapid lysis buffer + 10ug/ml Proteinase K, at 56°C, overnight Gennomic DNAs were extracte with Rapid lysis buffer containing 20 ug/ml of proteinase K overnight at 56°C. Library was prepaired with the HTGTS methods previously described (Chiarle at al., Cell, 2011_PMID:21962511; Genome-wide Translocation Sequencing Reveals Mechanisms of Chromosome Breaks and Rearrangements in B Cells) We induced DNA DSBs in intron 19 of ALK and we used linker forward primer and specific target reverse primers to capture functional fusions.