title: WGS of Diatraea saccharalis: adult male
design: The extractions were compared and the extraction from a single male moth was chosen for sequencing based on average fragment size (about 18.2 kb), quantity (620 ng), and integrity (visually on an agarose gel). A PCR-free shotgun strategy was used to prepare the genomic library with insert sizes ranging from 200 to 500 bp.