title: GSM5684112: PU.1_flox_3112; Mus musculus; RNA-Seq
library_construction_protocol: Epididymal adipocytes RNA were extracted using the RNeasy Lipid Tissue Mini Kit ( QIAGEN). The cells were homogenized in 1 ml QIAzol lysis reagent andcentrifuged at 12,000 g for 10 min at 4°C. The lysates under the lipid layer were transferred to a fresh tube and extracted with 200 μl chloroform, centrifuged at 12,000 g for 15 min at 4°C. The upper aqueous phase was transferred out and mixed with 1 volume of 70% ethanol. The samples were then applied to RNeasy Mini spin column and centrifuged at room temperature for 15 s at 8,000 g. The columns were washed once with 700 μl Buffer RW1, and twice with 500 μl Buffer RPE. The RNA samples were eluded with 30–50 μl RNase-free water.