title: GSM5047820: VEH-4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA (500 ng) from the striatum of injected mice was extracted using the RNeasy Mini Kit (QIAGEN; 74104) and underwent DNase treatment to prevent genomic DNA contamination. RNA integrity was assessed using RNA 6000 Nano Chips on a 2100 Bioanalyser (Agilent; 5067-1511). Samples with non-degraded RNA (RIN ≥ 8.5) were used for sequencing. Preparation of indexed cDNA sequencing libraries was carried out using the TruSeq poly-A mRNA method (Illumina; 20020594). Briefly, poly-A mRNA transcripts were captured from total RNA using poly-T beads and cDNA was generated using random hexamer priming. Paired-end sequencing (2 × 50 cycles) of indexed cDNA libraries was then carried out on a HiSeq 2500 machine (Illumina), generating at least 50 million reads per sample. Sequencing was performed using v4 SBS and Cluster Kits (Illumina; FC-401-4002).