title: GSM5911469: HepG2 cells, TFAM KD, H3K4me1; Homo sapiens; ChIP-Seq
library_construction_protocol: Crosslinking and fragmentation were conducted as described for the ChIP-pPCR. Chromatin fragments were incubated at 4 °C overnight with 2 µg of appropriate antibodies and immunoprecipitated with Dynabeads protein A/G (Life Technologies). After decrosslinking, the DNA was purified using a QIAquick PCR Purification Kit (Qiagen). The ChIP-seq library was prepared using a NEBNext Ultra DNA Library Prep Kit.