title: TPEE3
design: Microbiome profiling was conducted based on 16S rRNA gene sequences obtained from the rats fecal sample, it was amplified by universal bacterial PCR primers 27F (AGRGTTTGATYMTGGCTCAG) and 1492R (GGYTACCTTGTTACGACTT) by the Pacific Biosciences (PacBio) RS1 conducted in  ChunLab Inc. (Seoul, Republic of Korea)