title: Metatranscriptome from vaginal wash from Gyr Brazilian heifers
design: RNA was extracted using Maxwell viral total nucleic acid purification kit and cDNA was generated using Qiagen RT First Strand KIT. The second strand cDNA was synthesized by adding 20 l of Second Strand Marking Master Mix from Illumina Truseq Stranded mRNA library preparation kit and quantified using the Qubit Fluorometer. Library was prepped and sequenced by Omega Bio-tek, Norcross, GA.