title: Genome skimming: Astragalus calycosus var. calycosus - JLMC 9827
design: Fragmentation with Bioruptor Pico sonicator using 7 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i712 and i502; Index Read 1: AGCGATAG, Index Read 2: GCCTCTAT