title: GSM4878648: ChIP-seq VP16 HBM HA rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: For RNA-seq of tumor-derived samples, extraction was performed by Genewiz. Other RNA-seq extractions were performed using TRIzol. For ChIP-seq experiments, cells crosslinked in 1% formaldehyde were subject to sonication, and the resulting sheared chromatin was combined overnight with antibody. For RNA-seq of tumor-derived or MYC switchable cells (WT, 4A, VP16 HBM), rRNA depletion and library preparation were performed by Genewiz. For RNA-seq of untagged/HCF1N-tagged samples, library preparation was performed by Vanderbilt Technologies for Advanced Genomics (VANTAGE). ChIP-seq libraries were prepared using NEBnext Ultra II DNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina.