title: WGS of Lactiplantibacillus plantarum subsp. argentoratensis strain N6A: Greek sourdough
design: Samples were quality checked using Agarose Gel Electrophoresis and QUBIT 2.0. The genomic DNA was randomly fragmented by sonication, then DNA fragments were end polished, A-tailed, and ligated with the full-length adapters of Illumina sequencing, and followed by further PCR amplification with P5 and indexed P7 oligos. The PCR products as the final construction of the libraries were purified with AMPure XP system. Then libraries were checked for size distribution by Agilent 2100 Bioanalyzer, and quantified by real-time PCR (to meet the criteria of 3 nM). The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.