title: GSM5683315: scATAC-Seq StageI-0618; Homo sapiens; ATAC-seq
library_construction_protocol: The nuclei were isolated and washed according to the method supplied by 10X Gemomis: Nuclei Isolation for Single Cell ATAC Sequencing (CG000169). Then the nucleus were resuspend by chilled Diluted Nuclei Buffer (10xGenomics; 2000153). The volume of Diluted Nuclei Buffer used to resuspend nuclei was based on the number of starting cells and the final target nuclei concentration. Countstar (Rigel S2) was used to count the nuclei. The nuclei were then immediately proceed to single cell ATAC-seq libraries. Following the 10x Genomics single cell ATAC solution, by using Chromium Chip E Single Cell Kit(Product Code 1000156 ) and Chromium Single Cell ATAC Library & Gel Bead Kit (Product Code 1000110), the nucleus in bulk sample are partitioned into nanoliter-scale Gel Beads-inemulsion (GEMs), a pool of ~750,000 10x Barcodes is sampled to separately and uniquely index the transposed DNA of each individual nucleus, and libraries are generated (performed by CapitalBio Technology, Beijing). The libraries was sequenced using an Illumina Novaseq sequencer with a sequencing depth of at least 25k read pairs per nucleus with pair-end 50 bp (PE50) reading strategy (performed by CapitalBio Technology, Beijing).