title: GSM5856774: Whole transcriptome at normal salinity of NATL1A, rep2; Prochlorococcus marinus str. NATL1A; RNA-Seq
library_construction_protocol: Total RNA of each sample was extracted using TRIzol Reagent (Invitrogen)/RNeasy Mini Kit (Qiagen). Total RNA of each sample was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agarose gel. 1 μg total RNA with RIN value above 7 was used for following library preparation. Next-generation sequencing library preparations were constructed according to the manufacturer's protocol (NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®).