title: GSM5683406: mRNA_145; Homo sapiens; RNA-Seq
library_construction_protocol: After 24 hours, cells were lysed in RNA lysis buffer (Qiagen, Aus) and stored at -80°C until further extraction for transcriptomic analyses. RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo, Australia) according to manufacturer's instructions. RNA was extracted in groups of two patient samples per group per round to reduce batch effects. RNA was eluted into 30 μL RNase-free water and 24 μL was sent for sequencing NEBNext® Ultra Directional RNA Library Prep Kit for Illumina®