title: GSM5841294: Muscle biopsy_ID07_4h; Homo sapiens; RNA-Seq
library_construction_protocol: Muscle biopsies were obtained from the VL of the right leg using a 5-mm Bergström needle with manual suction following local anesthesia (2% Xylocitin). The location of all incision sites was randomized, and all incisions were 2-3 cm apart at the mid-portion of the VL muscle. After collection of the muscle tissue, the material was cleaned if there was excess blood using blotting paper and frozen in liquid nitrogen. Subsequently, all samples were stored at -80°C for later analysis RNA libraries were prepared for sequencing using standard BGISeq-500 protocols