title: GSM4820777: Mmu_lps_bal_cells; Mus musculus; RNA-Seq
library_construction_protocol: Bronchoalveolar lavage was performed by flushing lungs twice with 0.8 mL of Hanks solution containing 0.6 mM EDTA. BAL cells of eight animals were equally pooled for further analysis. Droplet-based single-cell partitioning of washed immune cells was performed using the 10X Genomics Chromium Controller. Single-cell RNA-Seq libraries were generated using the Chromium Single-Cell 3′ Reagent v2 Kit (10X Genomics, Pleasanton, CA) according to the manufacturer´s instructions.