title: GSM5093697: Sham-Animal 5; Mus musculus; RNA-Seq
library_construction_protocol: Bilateral whole hippocampus of SAH (n=4), Sham (n=3), and Naïve (n=3) groups were manually collected by an experimenter blinded to the specific group and total RNA was extracted immediately after using QIAzol reagent (RNeasy Mini Kit, Qiagen Inc, Hilden, Germany). RNA-seq was performed by the Genomics Core Laboratory of Weill Cornell University Medical College (New York, NY). Total RNA integrity was evaluated with the Agilent Bioanalyzer 2100 (Agilent Biotechnologies, Santa Clara, CA) and only samples with an RNA integrity number (RIN) >9.0 were further processed for RNA-seq.  RNA concentrations were measured using the NanoDrop system (Thermo Fisher Scientific, Inc., Waltham, MA). All RNA-seq libraries were prepared using 100ng of total RNA with the TruSeq Stranded Total RNA sample preparation kit (ribosomal RNA depletion and stranded RNA-Seq) with Ribo-Zero according to manufacturer's specified protocol (Illumina, San Diego, CA). Samples were multiplexed and sequenced across multiple lanes of a HiSeq 4000 Sequencing System (Illumina) using 50-base paired-end reads to achieve a minimum depth of 30 million aligned read-pairs