title: GSM5516389: 26 TNBC#10; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted from frozen sections of breast tumor samples using the  Nucleospin RNA set for Nucleozol kit according to the manufacturer's protocol (Macherey-Nagel). For breast tumor extracts, libraries were prepared using a modified version of the Takara SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian kit (Hendricks et al., in preparation). In brief, 100 ng of RNA at 10 ng/μL was sonicated using RL230 Covaris sonicator (Covaris Inc) and resulting material was confirmed using a Fragment Analyzer (Agilent). 10 ng of each sonicated sample was prepared using the pico input kit as for FFPE samples using a 1:8 volume reduction on the STP MosquitoHV. Final libraries were validated by Fragment Analyzer and qPCR prior to sequencing