title: GSM5097920: CA-5M-7; Candida albicans; RNA-Seq
library_construction_protocol: After 4 h of treatment, the media was removed and replaced with 2 mL PBS + 50 µg/mL DNase, which had been incubated at 37°C. Sterile cell scrapers were used to detach biofilm cells and technical replicates were pooled and mixed in a 15-mL Falcon tube, on ice. The biofilm cell samples were centrifuged at 3500 rpm, at 4°C, for 5 min, to form cell pellets. The supernatant was removed, and cell pellets were snap frozen in liquid nitrogen, before being stored at -70°C. Samples were packaged on dry ice and shipped to GeneWiz®, UK, for RNA extraction, sequencing and analysis. RNA extraction was performed using the QIAGEN Rneasy Plus mini Kit. 1) Ribosomal RNA depletion, 2) RNA fragmentation and random priming, 3) first and second strand cDNA synthesis, end repair, 5' phosphorylation and dA-tailing, 4) adapter ligation, PCR enrichment and sequencing. Sequencing: Illumina HiSeq, 2x150bp configuration, single index, per lane.