title: HiSeq X Ten paired end sequencing
library_construction_protocol: WGBS libraries were generated using the Pico Methyl-seq Library Prep Kit (Zymo Research, D5456). Briefly, input DNA was mixed with 0.1% unmethylated lambda DNA (w/w) (Promega, D1521), followed by bisulfite conversion. The bisulfite-converted DNA was then annealed with random primers for initial amplification, followed by adaptor ligation and final amplification. Libraries were purified by the Genomic DNA Clean & ConcentratorTM (Zymo Research, D4010), and the concentration was measured by Qubit? 2.0 Fluorometer with the qubit dsDNA High-sensitivity assay kit (Invitrogen, Q32854).