title: Illumina NovaSeq 6000 paired end sequencing; CircTulp4 functions in Alzheimer's disease pathogenesis by regulating its parental gene Tulp4
design: CircTulp4 functions in Alzheimer's disease pathogenesis by regulating its parental gene Tulp4
library_construction_protocol: Neuro-2a (mouse neuroblastoma N2a cells) cells were trypsinised using 1x Trypsin-EDTA (Sigma 59428C, 0.25% trypsin, 0.02% EDTA) and neutralised by the addition of FBS (Hyclone). The cell lines Neuro-2a (mouse neuroblastoma N2a cells) was grown in DMEM (GIBCO) supplemented with 10% FBS (HyClone) and 1% antibiotics. DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed in the Illumina Nextera DNA Library preparation kit.