title: GSM5684357: Uninjured adult zebrafish heart cells; Danio rerio; RNA-Seq
library_construction_protocol: After dissecting out the heart ventricles, they were digested by an enzyme cocktail containing collagenase and elastase at suboptimal temperature with continuous mechanical movement. Except digestion step the cells were kept on ice all the time and FACS sorted to isolate pdgfrb:EGFP+ cells. The libraries are constructed using the 10X Genomics 3' Gene expression kit v3.1 following manufacture's protocol. single cell RNA-seq.