title: GSM5093655: 35Spro:RIF #1 red receptacles rep1; Fragaria x ananassa; RNA-Seq
library_construction_protocol: Total RNA was isolated as previously described (Gambino et al., 2008; doi: 10.1002/pca.1078) from three biological replicates of receptacles after the removal of achenes for the control and transgenic lines. Illumina mRNA libraries were generated using the strand specific mRNA library as described (Zhong et al., 2011; doi:10.1101/pdb-prot5652) for 35Spro:RIF-RNAi and control experiment. For EXP2pro:RIF-RNAi, 35S:RIF and control experiment, the libraries were generated using TruSeq stranded mRNA according to the manufacturer's instructions (Illumina Inc., San Diego, CA, USA)