title: GSM5643734: Dnmt3A KO/KO_Cut&Run_Id4GFP_10dpp_H3K9me3_Replicate 2; Mus musculus; OTHER
library_construction_protocol: DNA was isolated with antibody and PA-MNase incubation. Library preparation was performed according to manufacturer's instructions (Accel-NGS 2S DNA library kit, Swift biosciences 21024, 21096 and adapters 28596) with a modified library amplification program: 98°C for 45sec, (98°C for 10sec, 60°C for 15sec, 68°C for 1min)x15 cycles, hold at 4°C.