title: GSM5093531: D7 ES Control Hi-C rep1_1; Mus musculus; Hi-C
library_construction_protocol: Hi-C ligated DNA were generated based on the in-situ Hi-C protocol (Rao et al., 2014) with minor modifications. The resulting in situ Hi-C DNA were sheared into fragments by sonication in microtube using a Covaris E220 (10 % duty cycle, 140 peak incident power, 200 cycles/burst, 110 s), subjected to double-size selection using AMPure beads (0.55X-0.8X) followed by biotin pull-down using 30 µL of Dynabeads MyOne Streptavidin C1 beads, and amplified with Illumina primers for 12 cycles of PCR to prepare for sequencing (paired-end 150bp).