title: GSM5683686: Cell-2 [20E]; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Invitrogen). Total RNA samples were first subjected to ribosomal RNA depletion using Ribo-zero rRNA removal kit, and subsequently treated with RNase R for 30 minutes at 37oC.  The resulting enriched circular RNA fraction were subjected to cDNA library construction using illumine kit.