title: GSM5911471: SH-SY5Y TRPM2 KO11 - untreated - replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Two control clones (Scr13 and Scr16) and two TRPM2 knockout clones (TRPM2 KO11 and TRPM2 KO28) were incubated with or without Doxorubicin (0.3 uM) for 24 h. Each condition was performed in duplicate, cells were collected and RNA was isolated using RNeasy Mini Kit (Qiagen) before being subjected to total RNA sequencing (RNAseq) The cDNA libraries were prepared using the NEXTflex™ Illumina Rapid Directional RNA-Seq Library Prep Kit (BioO Scientific) as per the manufacturer's instructions. Briefly, polyA RNA was purified from 200 ng of total RNA using oligo (dT) beads. The extracted mRNA fraction was subjected to fragmentation, reverse transcription, end repair, 3'– end adenylation, and adaptor ligation, followed by PCR amplification and SPRI bead purification (Beckman Coulter). The unique index sequences were incorporated in the adaptors for multiplexed high-throughput sequencing.