title: WGS of LCL NA20431
design: LCL samples were obtained from the Coriell Institute for Medical Research (Camden, NJ) as either DNA samples or cell cultures. The latter were cultured in Roswell Park Memorial Institute 1640 medium (Corning Life Sciences, Tewksbury, MA, USA), supplemented with 15% fetal bovine serum (FBS; Corning). Culture was maintained at 37C with 5% CO2 in a humidified atmosphere. DNA extraction was performed using the MasterPure TM Complete DNA &amp; RNA purification Kit following the manufacturers instructions.