title: Intestinal microbiota development in relation to nutritional status in preterm infants
design: Total DNA from stool samples of infants born preterm. DNA was extracted using the QIAamp Fast DNA Stool Mini Kit with the bead-beating method. The V3-V4 region of the 16S rRNA gene was amplified with dual-indexed primers and sequenced at the Roy J. Carver Biotechnology Center at the University of Illinois on the Illumina MiSeq 2x250bp platform.