title: 24h_1control
design: V3 and V4 regions of 16S rRNA genes were amplified, sequenced and analyzed by the STAB-VIDA company (Lisbon, Portugal) using the following primers: forward primer 5CCTACGGGNGGCWGCAG-3 and reverse primer 5-GACTACHVGGGTATCTAATCC-3. The library construction used was the Illumina 16S Metagenomic Sequencing Library Preparation protocol. The generated DNA fragments (DNA libraries) were sequenced with the lllumina MiSeq platform using MiSeq Reagent Kit v2 to produce paired-end sequencing reads (2250 bp). The analysis of the generated raw sequence data was carried out using QIIME2 v2018.2. The QIIME2 plugin for DADA2  was used to process the raw reads into amplicon sequence variants (ASVs) which provide higher phylogenetic resolution compared to operational taxonomic units (OTUs)