title: GSM5098056: U62_ctab; Elaeis guineensis; RNA-Seq
library_construction_protocol: Total RNA was extracted from leaf tissues using InnuPREP Plant RNA kit (Analytik Jena AG) or a CTAB-extraction method (Liao et al 2004, Prep Biochem Biotechnol 34:209–214). DNAse1 treatment was  carried out using an on column digestion method with RNeasy Mini kit (Qiagen) to remove genomic DNA carryover. Total RNA (500 ng) was used to generate RNA sequencing libraries using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen). Adapter sequences are Illumina compatible. RNA-Seq on Illumina platform to generate 7 million – 10 million SE50 (Agency L2 samples) or SE100 (L1 samples) reads.