title: GSM5048418: wet-AMD-1; Mus musculus; RNA-Seq
library_construction_protocol: Choroidal-retinal flat mounts were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.Then, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Based on the manufacturer's instructions, clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols