title: RNA-Seq of Piper longum
design: Total RNA was extracted from Piper longum and sequenced (Illumina & Nanopore) data is used for the data analysis. The De-novo hybrid transcriptome assembly is the main aim of the project. The Illumina data were demultiplexing using bcl2fastq and nanopore fast5 data were base-called using Albacore. The quality of the Illumina data was analyzed using FastQC. The short reads were processed to velvet de-novo assembly. Data from both Illumina and Nanopore platforms and a short read transcriptome assembly was submitted for hybrid transcriptome assembly using IDP-denovo Assembler.