title: GSM6102933: PRO-seq-03-PAF1AID-Aux2h-NHS1h; Homo sapiens; OTHER
library_construction_protocol: PRO-seq sample preparation was performed according to the previously described qPRO-seq protocol (Judd et al., 2020). ~1 million DLD-1 nuclei  mixed with spike-in Drosophila S2 nuclei were used for nuclear run-on assay. For PRO-seq, adapter sequences previously described (Mahat et al., 2016) were used. PRO-seq libraries were prepared according to the previously described qPRO-seq protocol (Judd et al., 2020).