title: sequencing of evolved S. Typhimurium clone
design: Nextera XT libraries were prepared for each of the samples. The barcoded libraries were pooled into equimolar concentrations following manufacturers guidelines (Illumina, San Diego, CA) using the Mid-Output Kit for paired-end sequencing (2150 bp) on an Illumina NextSeq500 sequencing platform. Raw data (mean virtual coverage 361x) was demultiplexed and subsequently clipped of adapters using Trimmomatic v0.38 with default parameters8