title: Illumina NovaSeq 6000 paired end sequencing; RNA sequencing of human iPSC-derived brain microvascular endothelial cells.
design: RNA sequencing of human iPSC-derived brain microvascular endothelial cells.
library_construction_protocol: Cells were harvested post-treatment. All media conditions were supplemented with 10 micromolar all-trans retinoic acid (RA). DMEM samples were differentiated in DMEM/F12 with glutamax (Thermo Fisher Product #10565018) as the basal media, and NB samples were differentiated in neurobasal medium (Thermo Fisher Product #21103049) as the basal medium. RA for differentiation days 4-6, including 24 h after purification Total RNA was extracted from harvested cells via Maxwell simplyRNA kit (Promega). RNA was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara), and cDNA libraries were constructed using the Low Input Library Prep Kit (Takara).