title: ZP5_2_2
design: For bacteria, the V3-V4 regions of the 16S rRNA gene were amplified using the universal primer sets 338F (5-ACTCCTACGGGAGGGAGGA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). For fungi, the internal transcribed spacer regions were amplified using the primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-13).