title: GSM5047933: P30_StrVasc_scRNA_Cisplatin; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspension from stria vascularis: Published protocol from 10x genomics was used to create a single cell suspension . Briefly, stria vascularis from ~6 P30 animals were isolated and collected in 3ml DMEM F-12 media. Following collection, 10 μL trypsin was added to the tube and incubated for 7 minutes at 37° C. After incubation 150 μL liquid was removed and replaced with 150 μL 10% FBS in DMEM.  The tissue was triturated for 2 minutes to dissociate single cells and then passed through a 20 μm filter.  200 μL of supernatant was removed, leaving a 100 μL final volume. Cells were counted in a Luna cell counter and a cellular density of 1x10^6 cells/ml was used to load onto the 10X genomics chip. Library preparation was performed according to the manufacturer's instructions for the 10x Genomics Chromium platform (10x Genomics). The libraries were sequenced on four lanes on a NextSeq500 sequencer (Illumina).