title: GSM5047361: FANS_RNA_group3_rep3; Mus musculus; ncRNA-Seq
library_construction_protocol: 10,000 INTACT- or FANS- nuclei were collected in 100 μl of RLT buffer followed by flash freezing. RNA was purified using the RNeasy Micro kit (Qiagen 74004) following the manufacturer's instructions. Libraries were prepared using NuGen Ovation SoLo RNA-Seq System following NuGen's standard protocol. Libraries were prepared with a starting amount of 1.23 ng and amplified in 13 PCR cycles.