title: GSM6032188: A549 cells,non-treated,rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction from non-treated as well as ISP-I treated NSCLC cells (3 replicates per group) was done by TRIzol reagent (Invitrogen, Carlsbad, CA, USA)and RNeasy Mini Kit (Qiagen, Valencia, CA, USA). The concentrations, quality as well as integrity of extracted RNA were assessed by NanoDrop spectrophotometry (Thermo Scientific). Sequencing libraries were constructed by TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA)