title: 16S rRNA V3-4 gene amplicon sequencing of Pore water sample (341F/785R) [JMF-1902-4-0187C]
design: Amplicon sequencing of Pore water sample. Sediment and pore water samples were taken from Elba, Italy and Sant Carles de la Rapita, Spain. Samples of sediment and pore water were also taken from aquaria conditions. DNA was extracted using the corresponding kit (PowerSoil or PowerWater). Sequencing was performed as described in https://doi.org/10.3389/fmicb.2021.669776. {primers: CCTACGGGNGGCWGCAG + GACTACHVGGGTATCTAATCC, nucleic acid: DNA, flow cell identifier: CBJCM, barcoding: UDB-H11, plate-position: 2 C6, barcodes: GAACTCGCTATG + GAACTTTAGCGC}.