title: GSM5268140: S15_Y_B_AT2_Saline_R3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested following manufactures directions for Arcturus PicoPure RNA isolation kit (Applied Biosystems) RNA-seq libraries were prepared from 3 ng total RNA using NEBNext RNA Ultra I kit (New England Biolabs, catalog number E7530L) with polyA mRNA isolation module (New England Biolabs, catalog number E7490S) (Samples 1–8). RNA-seq libraries were prepared from 0.35 ng total RNA using SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Samples 9–17).