title: RNA-seq of Arabidopsis thaliana root tips
design: Col-0 and ago10-1 seeds were germinated on 1 % sucrose,  MS plates. 6 Days after germination, seedlings were sprayed with 1 M BAP (or left untreated) and after 2.5 hours roots were cut 1-3 mm above root tip and immediately transferred to liquid Nitrogen. RNA extraction was performed using GeneMATRIX Universal RNA/ miRNA Purification Kit (Cat. No. E3599 Distributor Roboklon GmbH). Library preparation and sequencing were performed by a commercial service (Eurofins Genomics). Reads were aligned with RNA STAR