title: WGS of iPSC GM27730
design: Cells were grown on a layer of Mouse Embryonic Fibroblast feeder cells (Gibco) coated with D-MEM/F-12 supplemented with 20% of KnockOut Serum replacement (Gibco) and 10 ug/mL of Basic Fibroblast Growth Factor (bFGF) (Gibco, PHG0264). Feeder-dependent cells were transferred and adapted to Matrigel conditions following the recommendations of STEMCELL Technologies.  Cells were grown at 37C, 5% CO2, and passaged by dissociating to single cells with Accutase (Sigma, A6964) and plating at a density of 1x106 cells/well in Matrigel-coated 6 well plates. For the first 24 hours after passaging, cells were cultured with 10uM ROCK inhibitor (Y-27632; STEMCELL Technologies). DNA extraction was performed using the MasterPure TM Complete DNA &amp; RNA purification Kit following the manufacturers instructions.