title: Illumina HiSeq 2500 paired end sequencing; Transcriptome analysis of Beijerinckiaceae bacterium RH AL1 grown with pyruvate as the carbon source in response to lanthanum supplementation
design: Transcriptome analysis of Beijerinckiaceae bacterium RH AL1 grown with pyruvate as the carbon source in response to lanthanum supplementation
library_construction_protocol: Biomass was collected by centrifugation (10000 x g, 10 min, at room temperature). Pelleted biomass was flashfrozen and kept at -80 °C before being processed. Beijerinckiaceae bacterium RHAL1 was grown to exponential phase in minimal medium containing pyruvate as carbon source (0.2 % [w/v]) with and without lanthanum (1 µM) as growth supplement. Total RNA was extracted following a protocol modified after Wegner et al., 2013 (doi: 10.1016/j.margen.2012.12.001). Total RNA was enriched for mRNA by rRNA depletion using the MICROBExpress  bacterial mRNA enrichment kit (Thermo Fisher Scientific, Schwerte, Germany).  RNA-Seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Frankfurt, Germany).