title: GSM5778133: UNS_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA prep for mRNA-sequencing was performed using the miRNeasy (Qiagen) and treated with DNase I (Qiagen), according to the manufacturer's instructions. Quantities of extracted RNA were determined using NanoDrop2000 for cDNA synthesis or Agilent Bioanalyzer 2100 before RNA-Seq Library preparation. RNA-seq libraries were prepared with the Illumina TruSeq Stranded RNA Sample Preparation kit (Illumina) according to the manufacturer's protocol. Libraries were validated on an Agilent Bioanalyzer 2100.