title: GSM4777741: DEEP_br2_myod; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was extracted by QuickExtract (Epicentre) solution. Genomic DNAs from AAV9-sgRNA infected satellite cells were amplified using Q5 High-Fidelity 2× Master Mix (NEB). PCR products were purified through QIAQuick PCR purification kit (Qiagen) and subjected to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB).