title: RNA_Seq minigene SLC22A1 exon7 rs35854239 in Huh7 cells
design: SLC22A1 exon 7 of an rs35854230 carrier was introduced in the pSPL3b splicing vector, transfected into Huh7 cells and 48h later RNA was isolated, reverse transcribed and splicing products were amplified via PCR prior to RNA-Seq