title: Mali Subject ANON1014 IgM
design: Single probe-positive CD19+ CD10- IgM+ or IgG+ B cells were flow sorted from adult Malian PBMCs. The heavy chain of the BCR was amplified using reverse transcription and PCR followed by Sanger sequencing as previously described (Andrews et al., Immunity, 2019; Tiller et al., J. Immunol. Methods.  2008). Sequencing primers 3'IgGint GTT CGG GGA AGT AGT CCT TGA C or 3'IgMint GGA ATT CTC ACA GGA GAC GA. For complete method see Hopp et al., JEM, 2021 (doi 10.1084/jem.20200901).