title: GSM5684386: brain rJ2.2.8b-KO rep2; Mus musculus; RNA-Seq
library_construction_protocol: brain total RNA was harvested using Trizol reagent. mRNA was enriched by magnetic beads with Oligo (dT), RNAseq libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (E7530L, NEB). Libraries were sequenced on an Illumina Hiseq machine to obtain 150 bp paired-end reads. RNA libraries were prepared for sequencing using standard Illumina protocols