title: genome sequencing of Lednice Virus stock
design: The KAPA RNA HyperPrep Kit was used to prepare sequencing libraries from total RNA according to the manufacturers protocol using half-scale reactions and the Kapa Dual-indexed Adapter Kit (Kapa Biosystems). Pooled libraries were length-selected for 375500-bp fragments using a BluePippin 2% cassette (Sage Biosciences). If the length-selected concentration was less than 0.5 nM, additional PCR cycles were.