title: Illumina HiSeq 2500 paired end sequencing; RNA-Seq of human AML cell line, HL-60 and its mitoxantrone resistant subline HL60/MX2
design: RNA-Seq of human AML cell line, HL-60 and its mitoxantrone resistant subline HL60/MX2
library_construction_protocol: HL-60 and THP-1 was procured from National Centre for Cell Science (NCCS), Pune and HL-60/MX2 was purchased from ATCC. THP1-D7 resistant cell line was developed in the lab according to protocol mentioned in Salunkhe et.al. (14). All cell lines were grown in complete RPMI containing 10% FBS and 1% antibiotic cocktail (Penstrep). Whole transcriptome sequencing data analysis were performed as described earlier (6, 7). The differentially expressed genes were analyzed through fold-change based pre-ranked GSEA tool (8) for lipid metabolism pathways available from GSEA website. The p-value and FDR q-value cut-off of 0.05 was used for assessment. Whole transcriptome sequencing data analysis were performed as described earlier (6, 7). The differentially expressed genes were analyzed through fold-change based pre-ranked GSEA tool (8) for lipid metabolism pathways available from GSEA website. The p-value and FDR q-value cut-off of 0.05 was used for assessment.