title: GSM5822677: H446 (RNA-Seq); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using the Magzol Reagent (Magen, China)according to the manufacturer's protocol The mRNA was enriched by oligo dT according to instructions of NEB Next® Poly(A) mRNA Magnetic Isolation Module (NEB,USA). And then fragmented to approximately 200bp. Subsequently, the RNA fragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of  NEBNext® Ultra™ RNA Library Prep Kit for Illumina. Libraries were sequenced on the Illumina HiSeq xten with paired-end 150bp following the manufacturer's protocols.