title: GSM4728795: HC4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent and was further purified using Qiagen RNeasy Mini Kit. The RNA quality was assessed by formaldehyde agarose gel electrophoresis and was quantitated spectrophotometrically. Urine samples were collected from patients with bladder cancer and healthy volunteers and followed by 2,000g centrifugation for 10 min and 10,000g centrifugation for 30min at 4℃ to obtain the supernatant. Subsequently, the supernatant was centrifuged at 120,000g for 70min and washed again in PBS at 120,000g for 70min to obtain extracellular vesicles. The library was prepared with TruSeq Stranded mRNA LT Sample Prep Kit (Cat. No.15032612,Illumina) according to manufacturer's instructions.