title: GSM5683593: SARS-CoV-2_B.1.351_Infected_cell_totalRNA; Chlorocebus sabaeus; RNA-Seq
library_construction_protocol: RNA was isolated from Vero CCL-81 cells (non-infected and post 48h infection) using TRIZOL reagent (Invitrogen). RNA fragmentation followed by m6A RIP was performed with 15ug of RNA spiked-in with 9 ng of Bacterial RNA. Sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 (Pico Input Mammalian) from Takara and single-end sequenced (1X 88 bp) on Illumina NextSeq 2000 platform.