title: Illumina HiSeq 1500 sequencing; Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme
design: Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme
library_construction_protocol: 5 millions of cultured U87 cells were used for one extraction. miRNA extractions were performed using the NucleoSpin® miRNA kit (Macherey Nagel , France) according to the manufacturer's instructions. Libraries were prepared from 30 ng of small RNAs with the NEBNext Small RNA Library kit (Biolabs, France)