title: GSM5227738: hKO1(-)_rep2; Mus musculus; RNA-Seq
library_construction_protocol: hKO1(+) brain regions (hyphothalamus, midbrain, hindbrain) were micro-dissected and sorted by FACS. hKO1(+) and hKO1(-) cells were collected in TRIzol-LS. Total RNA extraction done using Direct-zol RNA microprep kit (Zymo Research) and eluted in 8uL for library construction. Library preparation was prepared using SMARTer Ultra-Low RNA Kit (Clontech) and cDNA amplified according to manufacturer's protocol.