title: WGS of E. faecium AUS0004 ahpgpxnprcat mutant
design: Genomic DNA of the npr and ahpgpxnprcat mutants was sequenced using the MiSeq Technology (Illumina, Inc., San Diego, CA) with paired-end and barcode strategy according to the NEBNext Ultra II FS DNA and the NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, MA). DNA was measured using a Qubit fluorometer (Thermo Fisher Scientific). The libraries were normalized, pooled, then loaded onto the Miseq Reagent Kit V3. Cluster generation and paired-end sequencing with single-index reads was performed in a 2  251 bp format.