title: GSM5664775: Chick_CB_MC_8; Gallus gallus; RNA-Seq
library_construction_protocol: RNA was isolated using the ARCTURUS PicoPure RNA Isolation Kit (ThermoFisher Scientific; Cat# KIT0204) according to the manufacturer's instructions and DNase treatment was done using RNase-Free DNase (Qiagen; Cat#79254). RNA was amplified one round using MessageAmp II aRNA Kit (ThermoFisher Scientific; Cat# AM1751). The RNA integrity was evaluated on the observation of a signature electropherogram pattern (Bioanalyzer). A minimum of 5-10 ng of amplified RNA was used per sample for the construction of each library. RNA-seq libraries were prepared by the National Research Council (NRC, Saskatoon) using the Illumina TruSeq RNA Sample Prep Kit v2 with the following modification: the protocol was started at the Elute, Prime, Fragment step using 5 µl amplified mRNA (the minimum amount was 5-10 ng mRNA as determined using Quant-iT RiboGreen RNA Assay Kit (Invitrogen)).  The quality of each cDNA library was checked on a DNA 1000 chip using the 2100 Bioanalyzer (Agilent Technologies Inc.). 