title: GSM5957787: ATAC-seq-UiNPC_L; Homo sapiens; ATAC-seq
library_construction_protocol: A total of 50,000-80,000 cell pellets were re-suspended in 50 μL lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) IGEPAL CA-630). Then the suspension was subjected to transposition reaction and purification with MinElute Kit (QIAGEN). After PCR amplification of transposed DNA fragments, the amplified libraries were purified using a Qiaquick PCR (QIAGEN) column. Then, purified libraries were assessed by gel electrophoresis and Qsep100 (Bioptic).