title: Metagenomes of fecal microbiomes from healthy US adults
design: Whole genome shotgun sequencing library preparation, library QC, quantification, and pooling were performed by DNA Technologies & Expression Analysis Core Laboratory at University of California Davis Genome Center. Library insert sizes were 250-280 bp and exact size selection of DNA fragments was performed with PippinHT (Sage Science). Ninety-six to 98 libraries were combined in a single pool for each sequencing batch. Library pools were size selected for 350bp-1kb, and paired-end sequenced on a NovaSeq6000 (Illumina) platform with S4 flow cell to result in an approximately 20 bp overlap in reads (PE 150 run).