title: Vitis_vinifera_L ---- 4 RNAseq
design: Total RNA from grape pulps was extracted as described in section 2.4, and then mRNA was enriched from the total RNA with oligo (dT) magnetic beads. The enriched mRNAs were fractured into short fragments, and random hexamers and reverse transcriptase were further added to synthesize the first- and second-strand cDNA. The cDNAs were amplied and size selected to construct a deep-sequencing library.