title: GSM6165850: hfp GLKD daughters; Drosophila melanogaster; ncRNA-Seq
library_construction_protocol: small RNA-seq: Total RNA was extracted from 10-20 pairs of ovaries using TRIzol (Life Technologies). A small RNA fraction of 15 nt to 30 nt in length was obtained following separation of total RNA on a denaturing polyacrylamide gel. This fraction was used to generate multiplexed libraries with Illumina TruSeq Small RNA library preparation kits (RS-200-0012, RS200-0024, RS-200-036 or RS-200-048) at Fasteris (http://www.fasteris.com). A house protocol based on TruSeq, which reduces 2S RNA (30 nt) contamination in the final library, was performed.