title: GSM6165307: HUVEC CRISPRi HIF1a-AS1; Homo sapiens; ATAC-seq
library_construction_protocol: 100.000 HUVECs were used for ATAC library preparation using Tn5 Transposase from Nextera DNA Sample Preparation Kit (Illumina). Cell pellets were resuspended in 50 µL PBS and mixed with 25 µL TD-Buffer, 2.5 µL Tn5, 0.5 µL 10% NP-40 and 22 µL H2O. The mixture was incubated at 37 °C for 30 min followed by 30 min at 50 °C together with 500 mM EDTA pH 8.0 for optimal recovery of digested DNA fragments. 100 µL of 50 mM MgCl2 was added for neutralization. The DNA fragments were purified with the MinElute PCR Purification Kit (Qiagen). Amplification of library together with indexing was performed as described above.