title: GSM6267742: Brunello_ACE2 293T_Spike_mNeonGreen_High_S4; Homo sapiens; OTHER
library_construction_protocol: The genomic DNA was extracted from the sorted cells using Thermo scientific GeneJet Genomic DNA purification kit (Catalogue# K0721). Following extraction of genomic DNA, sgRNAs were amplified for seuqencing. Equal molar amounts PCR gel-purified samples were pooled, denatured and diluted to 1.8pM, of which 1.3 ml was sequenced on a NextSeq 550 using a high output 75 cycle sequencing kit (Illumina)