title: WGS of C elegans: mixed population
design: Genomic DNA was extracted by digesting whole worms in proteinase K in Quiagen cell lysis buffer. Cell debris was cleared by adding Quiagen protein precipitation buffer and centrifuging. The supernatant was then subjected to IPA and ethanol precipitation. Reads from fastq files were adapter-trimmed using bbduk.sh from the bbmap package with the following options: -Xmx4g -ignorebadquality ktrim=r overwrite=true k=23 maq=20 mink=11 hdist=1. Reads were mapped to the C. elegans genome (WS251 build) using bwa-mem with default parameters