title: Amplicon sequencing of sample lib_AT3-D
design: This study developed novel DNA markers of multiple SNPs for the identification and genotyping of medical plants. The method combined targeted amplification and sequencing of selected genomic fragments, systematic bioinformatic analysis, and experimental validation. The method was applied to design 317  markers for identifying Artemisia argyi at the level of individual plants with 100% genotyping accuracy. Taking A. argyi as a case stud, this is the first example of developing effective DNA markers and an efficient pipeline for identifying medicinal plants, which can be readily extended to address various issues for herbal medicine, e.g., genetic analysis and control of genetic consistency.