title: GSM6031977: Liver WT rep2 [LAB_338_05]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from the liver was isolated using the TRIZOL reagent (Thermo Fisher Scientific). RNA quality was examined by RNA 6000 Pico kit (Agilent, Santa Clara, CA). An amount of 50 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA); 2x36 base paired-end sequencing was performed with NextSeq500 (Illumina, San Diego, CA) by Tsukuba i-Laboratory LLP (Tsukuba, Ibaraki, Japan). RNA libraries were prepared for sequencing using standard Illumina protocols