title: Nanopore long read cDNA-PCR sequencing of recombinant and mature UmaTR
design: Library prepared using total RNA extracted from U. maydis cells expressing a recombinant chimeric UmaTR construct. SQK-PCS109 PCR-cDNA library preparation kit instructions largely followed with modifications. RT reaction was performed using a mature UmaTR specific reverse primer and cDNA was amplified using 1 round of PCR to enrich for mature UmaTR and recombinant UmaTR sequences. Subsequent steps followed according to kit.