title: GSM5911400: +3xCTCF-TetO clone 1; Mus musculus; OTHER
library_construction_protocol: Genomic DNA (2 µg) was fragmented to an average of 500 bp by sonication (Covaris S220; duty cycle, 5%; peak power, 175 W; duration, 25 s). End-repair, A-tailing and ligation of full-length barcoded Illumina adapters were performed using the TruSeq DNA PCR-free kit (Illumina) according to the manufacturer's guidelines, with the exception that large DNA fragments were not removed In short, genomic DNA (2 µg) was fragmented to an average of 500 bp by sonication (Covaris) and ligation of full-length barcoded Illumina adapters was performed using the TruSeq DNA PCR-free kit (Illumina) according to the manufacturer's guidelines, with the exception that large DNA fragments were not removed. Libraries were pooled together and capture of desired fragments was performed using biotinylated probes against the piggyBac inverted terminal repeats (ITRs) sequences using xGEN Hybridisation reagents (IDT). Following capture, libraries were amplified for 14 cycles (KAPA HiFi Hotstart)