title: GSM5047547: S3_Cre_7d; Mus musculus; RNA-Seq
library_construction_protocol: The caudate lobe of the liver was snap frozen in liquid nitrogen and then stored in -80 until the day of the extraction. All 27 samples were extracted on the same day. Livers were homogenized in a Precellys Evolution homogenizer (Cat. Number P000062-PEVO0-A, “MET” programme) in 600µl buffer RLT/1% β-mercaptoethanol and RNA was extracted using the Rneasy kit from Qiagen (Dnase step included). The RNA concentration was determined by Nanodrop. 1μg RNA in 50μl Rnase-free water were used to prepare libraries using the TruSeq Stranded mRNA Kit.