title: GSM4728156: ZR-75-1, SRC-3 ChIP Veh Consensus; Homo sapiens; ChIP-Seq
library_construction_protocol: ZR-75-1 cells were fixed with 2mM disuccinimidyl glutarate for 20m followed by 1% formaldehyde for 10m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Plus for 10 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with 2.5µg of SRC-3 antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultra-Low Input Library Kit (Part# 180495). ChIP-Seq; Illumina NextSeq500 (High Output v2) single-end 75 bp reads.