title: GSM5047248: RNA-Seq Foxa2-KO GW4064 4; Mus musculus; RNA-Seq
library_construction_protocol: Liver RNA was isolated from Foxa2loxP/loxP;Alfp.Cre mice and control litter-mates. Quality of RNA samples was analyzed using Agilent RNA 6000 Nano Kit (Bioanalyzer, Agilent Technologies). Samples with RIN scores above 9.5 were used in library preparation. 1 µg of total RNA was used to isolate mRNA (NebNext Poly(A) mRNA Magnetic Isolation Module). Libraries of resulting mRNA were prepared using NebNext Ultra II RNA library preparation kit. All samples were sequenced on Illumina NextSeq 500.