title: GSM6102965: Hematopoietic progenitors, blf, 20hpf, rep2; Danio rerio; RNA-Seq
library_construction_protocol: At 20 hpf,  zebrafish embryos were dissociated into single cells with Librase treatment, RFP+ hematopoietic progenitors were purified with FACS, and RNA was extracted by using the Direct-zol RNA prep Kit (ZYMO Research, Cat. No. 2052). NEBNext Single Cell_Low Input RNA Library Prep Kit for Illumina (NEB, Cat No.6420) was used with 30,000 RFP+hematopoietic progenitors for the construction of sequencing libraries.