title: GSM4822509: Day 90 organoids Colonic Mock 1DPI Rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Following cell sorting for desired cell population, biological triplicate (n=3) samples of purified RNA was harvested using Qiazol and RNA Sample Preparation Kit v2 (QIAGEN). Total mRNA was isolated using magnetic beads-based poly(A) selection, fragmented, and randomly fragmented for reverse transcription, followed by synthesis of cDNA fragments. cDNA fragments were then end-paired, and ligated to Illumina Paired-End sequencing adapters. The products were end-paired and PCR-amplified to create the final cDNA library.