title: Archaea 16S rRNA sequencing of Ibiza salt
design: For 16S rRNA gene metagenomics analysis, the V3-V4 and V4-V5 regions were targets for bacteria and archaea, respectively. Primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3) were used to amplify bacterial 16S rRNA gene, while Arch519F (5-CAGCCGCCGTAA-3) and Arch915 (5-CGTGCTCCCCCGCCAATTCCT-3) were used for Archaeal 16S rRNA gene. PCR products were mixed at equal density ratios. The pool was then purified with Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated with NEBNext UltraTM DNA Library Prep Kit for Illumina and quantified via Qubit and q-PCR. Finally, the NovoSeq 6000 Sequencing System (2 x 300 bp) was employed for sequencing the samples at Novogene.