title: GSM5957409: RNAseq Phalange Rep 3; Gallus gallus; RNA-Seq
library_construction_protocol: For RNAseq library preparation, total RNA was isolated using Syngen Tissue RNA Kit The RNAseq library was prepared according to the Smart-seq2 method.In brief, cells were lised and poly(A)-RNA was reverse transcribed using oligo(dT) primers. The cDNA was pre-amplified with 18 cycles, followed by clean-up. Tagmentation was performed on 500 pg of cDNA. Then library was extended with Illumina adaptor sequences (PCR - 14 cycles). The library was sequenced according to the Illumina TruSeq v3 protocol on the HiSeq2500 with a single- read 50 bp and dual 9 bp indices.