title: RNA-Seq of Homo sapiens: CD34+ cells from bone marrow of immune aplastic anemia patient
design: Total RNA was extracted using the TRIzol reagent (Invitrogen) and purified with the RNA Clean & Concentrator kit (Zymo Research). Libraries were prepared with the TruSeq Stranded Total RNA with Ribo-Zero Gold Kit (Illumina) following the manufacturers recommendations. Briefly, cytoplasmic and mitochondrial ribosomal RNAs were depleted, first and second strands cDNA were synthetized, fragments were adenylated, adapters were ligated, and fragments were enriched using PCR.