title: GSM6165657: BN 1-LS RNAseq mTAL nz949; Rattus; RNA-Seq
library_construction_protocol: Kidneys were perfused with Hanks' balanced salt solution containing 20 mM HEPES (pH=7.4, 4°C). The kidneys were excised, the inner stripe of the outer medulla dissected, cut into small pieces, and incubated in digestion buffer for microscopy-guided manual selection of mTAL.   Isolated mTAL were isolated and flash frozen in liquid nitrogen.  Total RNA was extracted using a Trizol-based method and quantified by Nanodrop. RNA deep sequencing and analysis was performed as previously described by the Human Molecular Genetic Center at the Medical College of Wisconsin (Evans LC, et al.; Physiol Genomics, 2018;  PMCID: PMC6032288). RNA libraries were constructed on each individual animal using an Illumina TruSeq Stranded Total RNA Kit Samples were multiplexed on a 400 GB flow cell and pair-end sequenced using an Illumina HiSeq 2500.