title: GSM5582405: Ribo_4A1KO_PP242_1uM; Homo sapiens; OTHER
library_construction_protocol: Cells were directly lysed in lysis buffer on culture dishes. For ribosome profiling, RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification. For RNA-Seq, RNA extraction and TruSeq Stranded mRNA Library Prep Kit (Illumina). For RIP-Seq, pulldown by streptavidin beads, elution by biotin, RNA extraction, and TruSeq Stranded mRNA Library Prep Kit (Illumina).