title: GSM5957496: Metrnf/f LysM (cKO), rep2; Mus musculus; RNA-Seq
library_construction_protocol: We sorted LSK cells (Lin-Sca-1+c-Kit+) from two Metrnf/f and two Metrnf/f, LysM mice by FACS. After sorting, total RNA was extracted from the tissue using TRIZOL Reagent according the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined using 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA) and quantified using a Nanodrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MN, USA). Highquality RNA sample (OD260/280=1.8~2.2, OD260/230≥2.0, RIN≥6.5, 28S:18S≥1.0, >1μg) is used to construct sequencing library. RNA libraries were prepared for sequencing using standard Illumina protocols