title: GSM5048053: Caco-2-WT; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde for 10 mins at 37°C, washed in cold PBS, resuspended in lysis buffer, and sonicated to obtain chromatin fragments between 200 bp and 1000 bp. Sonicated chromatin was resuspended in IP buffer,  and incubated overnight at 4 °C with magnetic beads conjugated to CDX2 (Bethyl BL3194). The IP was washed 5 times with RIPA buffer and the DNA recovered by reversing the crosslink in 1% SDS, 0.1 M NaHCO3 for 8 h at 65°C. DNA was purified and quantified by Qubit. libraries were prepared for sequencing using standard Illumina protocols