title: GSM5351499: HCT116 USP1KO REP1; Homo sapiens; OTHER
library_construction_protocol: Frozen cell pellets were lysed in DNA lysis buffer containing 0.5% SDS and proteinase K overnight at 50 C, and then DNA was extracted using equal volume of phenol-chloroform and precipitated with ammonium acetate and ethanol. Okazaki fragment libraries were generated by PCR amplification and  primer dimers were  removed using SPRI beads (Beckman) before analysis by Tapestation (Agilent) and Illumina sequencing. Replication mapping was done using paired-end 50-bp with the NovaSeq 6000 Illumina sequencing platform and SP100flowcells.