title: GSM5952637: Day7_SNC_rep3; Mus musculus; RNA-Seq
library_construction_protocol: mice were perfused with ice-cold saline solution, sciatic nerve trunks harvested and kept on ice. On ice, nerves were minced and subsequentily digested in collagenase at 37°C for 45-60 min. To block gene transcription, actinomycin D was added to the digestion step.  Cell suspensions were loaded on a Percoll gradient, cells collected, and myelin depleted using a myelin depletion column. To enrich for immune cells, some samples were run over an anti-CD45 or anti-CD11b column (Miltenyi, 130-052-301). The Chromium Next GEM Single Cell 3' Reagent kit v3.1 (Dual Index) was used. Barcoding and library preparation was performed following the manufacturer's protocols. Briefly, to generate single-cell gel-bead-in-emulsion (GEMs) solution, approximately 15,000 cells, in a final volume of 43 µl, were loaded on a Next GEM Chip G (10x Genomics) and processed with the 10x Genomics Chromium Controller