title: Illumina NovaSeq 6000 paired end sequencing; Single-cell RNA-seq of Endometrial Superficial biopsies and full depth uterine tissue from organ donors
design: Single-cell RNA-seq of Endometrial Superficial biopsies and full depth uterine tissue from organ donors
library_construction_protocol: Endometrial biopsies were obtained from live donors with written informed consent from all participants recruited from the Newcastle Upon Tyne Hospitals after ethical approval (reference 16/NE/0167, North East - Newcastle & North Tyneside 1 Research Ethics Committee). Endometrial biopsies were obtained using a disposable endometrial cell sampler, starting from the uterine fundus and moving downward to the internal cervical ostium. None of the subjects were on hormonal treatments for at least 3 months prior to the procedure. RNA was converted into cDNA and amplified as part of the 10x v3 chemistry Libraries were constructed using the 10x v3 chemistry