title: GSM4728353: HAP1_WT-T14-B; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from screen cell pellets using the Wizard Genomic DNA Purification kit (Promega) according to manufacturer's instructions. Sequencing libraries were preparaed by amplifying gRNA inserts via a 2-step PCR using primers that include Illumina TruSeq adaptors with i5 and i7 indices.