title: GSM5048101: WT_PRL_R3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed with 1x PBS, placed on ice, and RNA was harvested using Trizol reagent and the PureLink™ RNA Mini Kit (Thermo Fisher Scientific, 12183018A) with Dnase treatment. mRNA library was prepared by polyA selection with ≥500 ng RNA using the Universal Plus mRNA-Seq Library Preparation Kit (NuGEN) following manufacturer's protocol.