title: GSM5048310: only_BMDE_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated with the total RNA isolation kit (Fluka, Sigma-Aldrich). 75 mM DTT was added to the lysis buffer to prevent RNA degradation. Samples were treated with RNAse free DNAse set (Qiagen) and RNA integrity was determined with a Bioanalyzer instrument (Agilent, Santa Clara, CA). The TruSeq Stranded mRNA Kit (Illumina, San Diego, CA) was used for cDNA library preparation. Libraries were 96 bp single-end sequenced on a HiSeq2500 platform (Illumina). TruSeq Stranded mRNA Kit (Illumina)