title: Iso-Seq of Aythya fuligula: adult female lung
design: Two micrograms of total RNA from each sample in four parallel reactions were converted to cDNA using the Teloprime full-length cDNA amplification kit (v1) according to the manufacturer's instructions (013, Lexogen, Vienna, Austria). After end-point PCRs, all samples were tested for quality and quantity. The product size distribution was visualised using an Agilent 2200 Tapestation using D5000 screen tape. The libraries' concentration was measured on a Qubit 3 (Thermo Fisher Scientific, Massachusetts, USA) with high-sensitivity DNA reagents. Technical replicates were pooled and selected for PacBio Iso-Seq assays.