title: ChIP-seqofArabidopsisthaliana:seedlingH3_atx3/4/5_4
design: Chromatin was extracted from whole seedlings grown for 15 days on MS media and sheared with covaris sonicator. Antibodies used for immunoprecipitation are; H3 (ab1791; Abcam), H3K4me1 (ab8895; Abcam), H3K4me2 (ab32356; Abcam), H3K4me3 (ab8580; Abcam), H3K36me2 (MABI0332; MBL), H3K36me3 (MABI0333; MBL), RNAP2 total CTD (CMA601; MBL), RNAP2 phospho S2 (CMA602; MBL), RNAP2 phospho S5 (CMA603; MBL), FLAG (F1804; SIGMA). Libraries were made with KAPA Hyper Prep Kit (Kapa Biosystems), and dual size-selected using Agencourt AMPure XP (Beckman Coulter) to enrich 200  500 bp fragments. The libraries were 50 bp single-end sequenced by the HiSeq4000 sequencer.