title: GSM5047177: sRNA_sequencing_Aphid_72hrs_bioreplicate_3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Life Technologies) following the manufacturer instructions. mRNA for RNA and PARE sequencing was obtained by purification with the NEB mRNA isolation kit (New England Biolabs). RNA for sRNA library preparation was enriched with the mirVana miRNA Isolation Kit (Life Technologies). Genomic DNA was extracted using the DNeasy Plant Mini Kit (Qiagen). sRNA libraries were produced using the TruSeq Small RNA Sample Preparation Kit (Illumina). PARE libraries were constructed according to Zhai et al (2014). RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Bisulfite DNA libraries were produced and sequenced at Novogene (Hong Kong).