title: GSM4728861: Aptamer21-DMS-control-1; Synthetic plasmid; OTHER
library_construction_protocol: All Aptamer 21 derivatives were produced by in vitro transcription (IVT) from linearized plasmid templates, using the HiScribe IVT kit (New England Biolabs) according to the manufacturer's standard protocol with 33.5 ng/µL template. Total RNA was extracted from 12 samples of 2x10^5 to 1.5x10^6 HepG2 cells using the ReliaPrep RNA cell miniprep system (Promega) according to the manufacturer's standard protocol. Libraries were produced using standard Illumina protocols.