title: RNA-seq of MDA-MB-231 shNT rep 2
design: Total RNA from MDA-MB-231 shNT (control) and mutant TP53-silenced (sh1 and sh2) cells were extracted using the RNeasy Plus Mini Kit (QIAGEN) according to protocol instructions. Extraction was followed by DNase digestion (Turbo-Dnase, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) and RNA quality was assessed by using an agarose gel electrophoresis after RNA exposure to 70C for 5 minutes. Truseq Stranded mRNA Sample Prep Kit was used for library preparation and Illumina sequencing  by IGA (Udine, Italy).