title: GSM5911353: HiC auxin treatment 90 min; Mus musculus; Hi-C
library_construction_protocol: Cells were crosslinked with 1% formaldehyde (EMS, 15710) and quenched with 0.125 M glycine for 5min at RT. Cells were lysed in 10 nM Tris-HCl pH 8.0, 10 nM NaCl, 0.2% NP-40 (Sigma-Aldrich, I8896-50ML), complete protease inhibitor (Sigma-Aldrich, 11836170001). Library preparation was performed according to NEBNext Ultra DNA Library prep kit instruction (NEB, E7370L) and samples were purified with magnetic AMPure bead (Beckman Coulter, A63881).