title: GSM4878551: YM_Blue1_S12: CD62Lhi_rep1; Mus musculus; RNA-Seq
library_construction_protocol: mRNA was extracted using RNeasy Plus Micro kit  (Qiagen) following manufacturer's protocol and eluted in 14μl of RNAse free water.  mRNA quality was assessed on a 2200 TapeStation (Agilent Technologies) using  High Sensitivity RNA ScreenTape (Agilent Technologies).mRNA from each  biological replicate (5ng/sample) was converted into cDNA and amplified (9 cycles)  using the SMART-Seq V4 Ultra Low Input RNA Kit (Takara Bio). 10ng of cDNA  was used to prepare DNA libraries using Nextera XT DNA Library Prep Kit  (Illumina). Barcoded samples were pooled, diluted at 1.8pM, loaded onto a TG  NextSeq 500/550 High Output Kit v2 (150 cycles, 400M reads, Illumina) and  paired-end sequencing was performed on a NextSeq 550 (Illumina)