title: GSM5097104: clonal hematopoiesis-positive, HTP0241A2; Homo sapiens; ssRNA-seq
library_construction_protocol: Whole blood samples were collected into PAXgene Blood RNA Tubes (PreAnalytiX/Qiagen) and RNA purified using a PAXgene Blood RNA Kit (Qiagen). RNA quality was assessed using an Agilent 2200 TapeStation and quantified by Qubit (Life Technologies). Globin RNA depletion, poly-A(+) RNA enrichment, and strand-specific library preparation were carried out using a GlobinClear kit, NEBNext® Poly(A) mRNA Magnetic Isolation Module, and NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina. Paired-end 150 bp sequencing was carried out on an Illumina NovaSeq 6000 instrument by Novogene Co., Ltd.