title: GSM5093703: Human primary epithelial cells, E.g. stimulated, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Growth medium was carefully removed and 500 µl 2.5% paraformaldehyde was added and incubated for 1 hour at room temperature. Subsequently, 2ml pure water was added to dilute the fixation solution to 0.5%. The samples were kept in 24-well-plate and sealed with paraffin film. Total RNA was extracted from the cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. 500-1000 ng of total RNA of the transfected cell cultures of pGECs and pGFs was sequenced with 16 million reads (75 bp single end) on a NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 Cycles). Single-end, 75 bp