title: RNA extraction of complete central nervous system (CNS) of one single adult of Locusta migratoria
design: Total RNA was extracted from the CNS of single adult locusts(L. migratoria) kept at 4 C during the preparation. CNS sampleswere preserved in RNAlater solution (QIAGEN GmbH, Hilden, Germany)and sequenced using the Illumina Truseq RNA SamplePreparation Kit (Illumina, San Diego, USA) at Beijing GenomicsInstitute (China). Isolation of mRNA, generation of cDNA and PCRamplification of suitable fragments were performed as described inRef. [4]. Rawdata were initially filtered by removing adapters, readswith more than 5% of unknown basses and reads with low qualitysequences (reads having more than 20% bases with quality valuelower or equal to 10). Transcripts were then de novo assembledusing Trinity [14] with default options and used for tBLASTn on alocal computer.