title: Draft Genome Sequence of Streptomyces rochei Strain NS1
design: Nextera XT Library Preparation Kit (Illumina, San Diego, USA) was employed for genomic DNA libraries preparation following the manufacturers protocol with some modifications: For PCR, two nanogram of DNA instead of one was used as input and elongation time was exceeded from 30 seconds to 1 min. Hamilton Microlab STAR automated liquid handling system was employed for DNA quantification and library preparation. By using Roche light cycler 96 qPCR machine pooled Illumina libraries were generated and quantified in the Kapa Biosystems Library Quantification Kit. Illumina HiSeq platform was used for base calling by 250bp paired end protocol. For all specified soft-wares default parameters were used.Pair end reads were checked for quality control and adapted reads were trimmed by using Trimmomatic 0.30 with a sliding window quality cutoff of Q15 and quality was evaluated by means of house scripts in combination with three soft-wares i.e. Samtools, Bedtools and bwa-mem. By the use of SPAdes version 3.7 de-novo assembly was performed (10) and annotation of contigs were done by using Prokka 1.11 . Quast was used to assemble metrics.