title: GSM5047535: OPA1-TET2; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were collected from sonicated cells lysed with ChIP lysate buffer. The DNA was mixed with end repair mix and incubated at 20℃ for 30 minutes. The end-repaired DNA was purified using the QIAquick PCR Purification Kit (Qiagen). Then A-tailing mix was added and the mixture was incubated at 37℃ for 30 minutes. The purified DNA, adapter and ligation mix was incubated at 20℃ for 15 minutes, and the adapter-ligated DNA was purified with the QIAquick PCR Purification Kit. After PCR amplification, the PCR products (about 100-300 bp) were separated through agarose gel running, and purified with QIAquick Gel Extraction kit (QIAGEN). The library was qualified with Qubit ssDNA kit. The sequencing was performed with the BGISEQ-500 sequencing system by BGI-Shenzhen, China.