title: GSM5047508: Sample 18: empty vector control; high density – Biological replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: The cells from two wells were combined and collected for RNA extraction 11 days (high) and 14 days (low) after plating. RNA was extracted using RNeasy mini kit (Qiagen, Hilden, Germany, cat # 74106) according to the manufacturer's protocol. The QIAGEN RNase-Free DNase Set was used for on-column DNA digestion according to the manufacturer's protocol (Qiagen, Hilden, Germany,  cat # 79256). Total RNA samples were sent to BGI Group, Beijing, China, for library preparation and sequencing. Reads (single-end 50bp at a sequencing depth of 20 million reads per sample) were generated by BGISEQ-500.