title: WGS of iPSC GM27622
design: Cells were grown in mTeSR Plus Basal medium at 37C, 5% CO2, and passaged by dissociating to single cells with Accutase (Sigma, A6964) and plating at a density of 1x106 cells/well in Matrigel-coated 6 well plates. For the first 24 hours after passaging, cells were cultured with 10uM ROCK inhibitor (Y-27632; STEMCELL Technologies). DNA extraction was performed using the MasterPure TM Complete DNA &amp; RNA purification Kit following the manufacturers instructions.