title: Hc3 tentacle Hi-C (DpnII)
design: A Hi-C library constructed from the tentacle of Hormiphora californensis individual Hc3. The library was constructed using the DpnII enzyme, the ends were filled in with dATP, dGTP, dTTP, and biotinylated dCTP. The blunt ends were ligated overnight at 16C with shaking. After proteinase K digest, and SPRI bead cleanup, 100ng of DNA in 26L of H2O was cleaved in one NEB FS kit reaction for 17.5 minutes using the standard thermal cycler protocol. Adapters were ligated to the fragments using the standard NEB protocol. Biotinylated molecules were captured using 25L of MyOne C-1 beads, beads were washed according to the 4DN protocol, then amplified for 10 cycles with Kapa HiFi Hotstart with indexed p5 and p7 adapters. Reads were demultiplexed using the dual indices. These are the raw reads off of the machine after demultiplexing.