title: NextSeq 550 paired end sequencing; RRBP1 rewires Cisplatin resistance in Oral Squamous Cell Carcinoma by regulating YAP-1
design: RRBP1 rewires Cisplatin resistance in Oral Squamous Cell Carcinoma by regulating YAP-1
library_construction_protocol: The RNA-seq library preparation was performed for H357 CisR Nt Sh and H357 CisR CMTM6 shRNA treated with vehicle control (UT) and cisplatin (10 μM) (CisT) for 24 h.  The cells preserved in LBP lysis buffer were first taken out from -80°C and thawed by placing the plates/tubes on ice. Total RNA was isolated using NucleoSpin RNA Plus kit (Macherey-Nagel) according to manufacturer's protocol. RNA concentration was estimated by Nanodrop (Thermo) and then 2ug of total RNA was used to prepare library. For RNA-seq library preparation 2 μg of total RNA was used to isolate mRNA through magnetic beads using mRNA isolation kit (PolyA mRNA isolation Module, NEB) followed by RNA-seq library preparation using mRNA library preparation kit (NEB) strictly following the vendor recommended protocol.