title: GSM5684316: WT 5' SLAM-seq 2 h rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Polysome samples were prepared by lysis in polysome lysis buffer. Following fractionation, RNA was extracted from sucrose by the addition of 50 mM EDTA and an equal volume TRIZOL LS, and homogenised for 20 minutes at 50°C with frequent mixing. For 5'UTR-SLAM-seq, 4SU-containing RNAs were directly extracted by a standard TRIZOL protocol. ReCappable-seq