title: Illumina HiSeq 1000 paired end sequencing; RNA-seq analysis of crypt samples isolated from the small intestine of KO and control mice
design: RNA-seq analysis of crypt samples isolated from the small intestine of KO and control mice
library_construction_protocol: Crypts were isolated from the small intestines of Bach2 KO and control mice (n=3, respectively) 10 days after tamoxifen administration.  Total RNA was extracted for sequencing using RNA-Seq.RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).A total amount of 3ug RNA per sample was used as input material for the RNA sample preparations.   Sequencing libraries were generated using NEBNext® (NEB, USA) following manufacture's recommendations and index codes were added to attribute sequencing to each sample.