title: scRNA-seq on human: gastric cancer
design: The Countess II Automated Cell Counter was employed to count the cell number, to evaluate cell viability and to adjust the concentration of single cell suspensions. The Single Cell 3 Protocol was applied to generate thousands of nanoliter-scale Gel Bead-In-Emulsions (GEMs). Cell lysate, reverse transcription and full-length cDNA synthesis were performed during the incubation of GEMs. Full-length barcoded cDNAs wereamplified by PCR to generate sufficient mass for library preparation. Library preparation was followed by the manual of Chromium single cell 3 kit. The single cell RNA libraries were sequenced on the Illumina HiSeq4000 using 150bp pair-end reads by Genedenovo Biotechnology Co., Ltd (Guangzhou, China).