title: GSM5514732: Day 15 RNA-seq KO 2; Drosophila melanogaster; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq: FACS-enriched beta cells were pooled with a 10% spike-in of S2 cells. RNA was isolated using Trizol. RNA libraries were prepared from 400ng of total RNA per sample using poly-A capture beads and NEBNext directional RNA-seq library prep kit for Illumina (E7760). scRNA-seq libraries were prepared using Chromium Single Cell 3' protocol with Reagent Kit v3.1 (10X Genomics)