title: Bemisiatabaci:singlebacteriocytetranscriptome
design: Thesinglecellsampleswerecollectedintubeswithlysiscomponentandribonucleaseinhibitor.ThenwecarriedouttheamplificationbytheSmartSeq2method.ThenthecDNAproductionwascheckedbyQubit3.0FlurometerandAgilent2100Bioanalyzer.ThenthecDNAwasshearedrandomlybyultrasonicwavesforIlluminalibrarypreparationprotocolincludingDNAfragmentation,endrepair,3endsAtailing,adapterligation,PCRamplificationandlibraryvalidation.