title: RNA-seq of Mus Muculus: transplanted TCh3-2
design: TAb2 and TCh3 tumor cells were cultured in DMEM complete media and collected for RNA purification.  Total RNA was purified with TriPure (Roche) and cleaned up with RNAeasy Kit (Qiagen) according to manufacturers instructions. RNA samples were then depleted of ribosomal RNA and subjected to pair-ended RNA sequencing by NovaSEQ 6000 (University of Colorado at Anschutz Genomics and Microarray Core).  Raw sequencing data with adapter sequences were filtered using BBduk from BBtools (version 38.86) to remove adapter contamination and obtain clean data for subsequent processing.