title: Whole exome sequencing from rectal cancer patients
design: Five hundred nanograms of DNA were used for the construction of libraries (Agilent SureSelect Human All Exon v6 kit, Agilent Technologies, USA) and sequencing was performed on NextSeq 4000 (Illumina, USA) to generate paired end reads (2 x 100 bp), with at least 50x average vertical coverage (Macrogen, Seoul, South Korea).