title: GSM5911542: CITE-seq WT replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Embryos were dissected at E10.5 and AGM were processed for flow cytometry. Cells were stained with CD31-BV605 and the following CITE-seq antibodies at a final concentration of 0.2 ug/100 µL: TotalSeq™-A0012 anti-mouse CD117 (BioLegend #105843), TotalSeq™-A0443 anti-mouse CD41 (BioLegend #133937), TotalSeq™-A0110 anti-mouse CD43 (BioLegend #143211), TotalSeq™-A0157 anti-mouse CD45.2 (BioLegend #109853), TotalSeq™-A0191 anti-mouse/rat/human CD27 (BioLegend #124235), and TotalSeq™-A0073 anti-mouse/human CD44 (BioLegend #103045). Cells from 2-5 embryos were pooled for any given samples based on genotype Libraries were prepared using the 10X Chromium Single Cell 3' Reagent v3 chemistry kit as per the standard protocol from 10X Genomics (Pleasanton, California)