title: RNA-Seq of Porphyromonas gingivalis W83
design: P. gingivalis strain W83 and its derivatives were inoculated in TSBHK and grown for approximately 24 h. The cultures were sub-cultured in pre-reduced TSBHK and grown to an OD600 of 1.0. Cultures were diluted 1/5 with fresh pre-reduced TSBHK, and 10 ml aliquots of each culture were spotted on blood agar plates. After 24 h incubation, cultures were scraped off the plates and the RNA extraction was performed, and these samples were processed and submitted for RNA sequencing using an Illumina platform.