title: GSM5097993: PAO1161 EV_1_control_for_PA3458_RNA-seq; Pseudomonas aeruginosa; RNA-Seq
library_construction_protocol: Total RNA was isolated from three independent replicates of each strain. 2 ml of cultures which reached optical density 0.4-0.6 at 600 nm were mixed with 4 ml of RNAprotect Bacteria Reagent (Qiagen). RNA was isolated with an RNeasy mini-kit (Qiagen) according to the manufacturer's protocol. Total RNA was digested with DNase (TURBO DNA-free Kit, Ambion) to eliminate genomic DNA. rRNA was depleted using Ribo-Zero rRNA Removal Kit (Bacteria) (MRZMB126,Illumina) according to manufacturer instructions. Libraries were prepared according to instructions accompanying the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, E7370S).