title: ATAC-seq of Danaus plexippus : mid-fifth instar Hind imaginal discs caterpillars
design: For each library, 3-4 larvae were dissected in ice-cold PBS, and isolated tissues were homogenized with a dounce homogenizer. Homogenate was then dyed with trypan blue and nuclei with counted using a hemocytometer. Lysis and transposition were done according to the original ATAC-seq protocol (Buenrostro et al. 2013). Qiagen PCR cleanup kits were used to clean transposed DNA, followed by PCR amplification for 10-11 cycles, and a final PCR cleanup kit purification step. Libraries were pooled and sent to sequence at the BRC Genomics Facility at Cornell University, for 2 x 37 bp paired-end read sequencing.