title: GSM5550341: RCHAcV_RA_SCM_Pos_MTX; Homo sapiens; RNA-Seq
library_construction_protocol: After the treatment conditions, RNA was extracted using the RNAeasy mini kit (cat. no. 74104) from Qiagen. 200-1000 ng of total RNA was used with the KAPA mRNA HyperPrep kit with polyA selection beads and 10 rounds of PCR to generate RNA-seq libraries.