title: Complete central nervous system of one 5th Juvenile and one Adult
design: Libraries were prepared using 1 g of total RNA with the IlluminaTruSeq stranded RNA sample preparation Kit. The polyA containingmRNA molecules were first purified using oligo-dT attached magneticbeads and then fragmented and primed for cDNA synthesis. FragmentedRNA underwent reverse transcription using random hexamers producingfirst strand cDNA. This was followed by the second strand cDNAsynthesis with DNA polymerase I and RNase H. After end repair and Atailing,multiple indexing adapters for hybridization were ligated onto aflow cell. Subsequently, products were enriched with PCR and purifiedto create the final cDNA library using single primer isothermal amplificationSPIA (NuGEN, CA, U.S.A). Library was validated and quantifiedusing the Agilent 2100 Bioanalyzer.