title: non-diabetic (dbm) control replicate 5
design: One microgram of total RNA samples was sent to Novogene (Sacramento, CA) for mRNA sequencing. RNA-seq fastQ files were filtered and trimmed from adaptors using Trimmomatic algorithm 36. The reads were aligned to Mus musculus genome assembly and annotation file GRCM38:mm10 using STAR algorithm 37. Gene expression was estimated in FPKM counts using RSEM algorithm 38.