title: GSM5957329: Total Kidney 13 week old WT2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from freshly frozen kidneys of three age- and sex-matched WT and Clr-f−/− mice at 7, 13, and 24 weeks of age using TRIzol (Thermo Fisher Scientific) and RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA).  RNA-seq was performed on the Illumina HiSeq 4000 platform, with paired-end reads, at > 30 × 10^6 read depth per RNA libraries were prepared for sequencing using standard Illumina protocols