title: Whole Genome Seq of Mus musculus: 129S6/SvEvTac
design: Genomic DNA sample was randomly fragmented by Covaris technology and the fragment of 350bp was obtained after fragment selection. The end repair of DNA fragments was performed and an "A" base was added at the 3'-end of each strand. Adapters were then ligated to both ends of the end repaired/dA tailed DNA fragments, then amplification by ligation-mediated PCR (LM-PCR), then single strand separation and cyclization. The rolling circle amplification (RCA) was performed to produce DNA Nanoballs (DNBs). The qualified DNBs were loaded into the patterned nanoarrays and pair-end read on the BGISEQ-500 platform.