title: Genome sequencing of Bifidobacterium longum subsp. longum BCRC 12585
design: The genomic DNA of Bifidobacterium longum (subsp. Longum, BCRC 12585) was extracted from the enriched medium culture. Genomic DNA library constructed for Illumina sequencing was fragmentized to ~550bp by ultrasonicator and then conjugated the Truseq indexed adapters using TruSeq DNA PCR-free LT sample preparation kit (Illumina, USA). Sequencing was performed on an Illumina MiSeq using the V3 Reagent kit with 300bp paired-end configuration by Health GeneTech Corp. (Taoyuan, Taiwan).