title: The RAD-seq library for Megacodon set8
design: Genomic DNA was extracted using a Plant Genomic DNA kit (Tiangen Biotech, Beijing, China) following the kit protocol. Restriction site-associated DNA library followed a universal and simplified ddRAD library in angiosperm plants named as MiddRAD (Yang et al., 2016). Whole genomic DNA (20 ng) was digested by MspI (New England Biolabs, NEB, #R0106L) and AvaII (New England Biolabs, NEB, #R0153L) enzyme at 37  for 3h in a 20 ul reaction volume buffered with 1CutSmart buffer (New England Biolabs, NEB, #B7204S). And fragment library of 400-600 bp was performed strictly according to the protocol of MiddRAD. The Illumina HiSeq X Ten (San Diego, CA, USA) sequencing platform (PE150) was used for sequencing at Novogene Co. (Beijing, China).