title: GSM5048187: S2_input_rep2; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Reverse cross-linking of chromatin was carried out overnight with 5 M NaCl at 65 °C followed by proteinase K treatment for 2 h at 45 °C and reverse cross-linked chromatin was extracted using phenol–chloroform followed by ethanol precipitation. 1. DNA-end repair,3'-dA overhang and ligation of methylated sequencing adaptor.  2. PCR amplification and size selection (usually 100-300bp, including adaptor sequence).