title: RNA-seq of CMs from Individual GM19209 treated with VitaminD
design: Poly-adenylated mRNAs were isolated from thawed lysates using the Dynabeads mRNA Direct Kit (Ambion) and following manufacturer instructions. RNA-seq libraries were prepared using a protocol modified from the NEBNext Ultra II Directional RNA library preparation protocol to use 96 Barcodes from BIOOScientific added by ligation. Library pools were sequenced on a NextSeq 500 using 75bp PE reads.