title: GSM5683365: siLATS1/2 M01 R1 [siLATS_1]; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were prepared as technical duplicates and  total  RNA  was  isolated  using  the  miRNeasy  Mini  Kit  (Qiagen)  with  on-column  DNA digestion using RNase-Free  DNase  Set  (Qiagen)  according  to  the  manufacturer's instructions. RNA sequencing libraries were prepared from total RNA using poly(A) enrichment using 200 ng input RNA with TruSeq stranded mRNA Sample prep (Illumina). QC was performed on a fragment analyzer using DNF-473-33-SS NGS Fragment 1-6000bp kit (Agilent). Libraries were sequenced with an Illumina NovaSeq 6000 system with Single Read conditions (Read 1: 101/Index 1 : 8/Index 2: 8).