title: GSM5953394: AP-03; Mus musculus; RNA-Seq
library_construction_protocol: For RIP-seq,TRIzol reagent was added directly to the beads for HuR-bound RNA isolation and processed for library preparation. For RNA-seq TRIzol reagent was added directly to the cells pellets RNAs from INPUT and RIP were tested for quality using Bioanalyzer and 7ng of the extracted RNA, having RIN higher than 9, were used for fragmentation at 94 °C for 4 min. RNA libraries were generated using the SMART-Seq Stranded Kit (Takara).