title: GSM5911553: Os18SrRNA DMS(+)Vacuum2%; Oryza sativa; OTHER
library_construction_protocol: DMS-treated or DMS-Untreated samples were pelleted and resuspended in 1 mL Trizol, RNA was extracted by phenol-chloroform extraction and isopropanol precipitation.rRNA was subtracted with Ribo-zero rRNA removal kit for plant (illumina). For target-specific DMS-MaPseq, 6 µg of DMS-treated sample was mixed with gene-specific RT primers. The reverse transcription was proceeded with TGIRT-III (Ingex). Then specific gene cDNA were amplified using KOD hot start DNA polymerase with gene-specific primers. For genome-wide DMS, DMS-MaPseq library was constructed using Illumina TruSeq® Stranded Total RNA Sample Prep Plant kit (Illumina) and TGIRT enzyme (InGex).