title: GSM5683877: THP-1 shPIWIL4_1 (exp. rep1); Homo sapiens; RNA-Seq
library_construction_protocol: 1x106 THP-1 cells transduced with either scrambled or shRNA against PIWIL4 were sacrificed, washed with PBS and resuspended in 1mL TRIZOL reagent.  RNA was harvested and Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols