title: The RNA-seq library for Beesia calthifolia BC4
design: The TRIZOL reagent (Sigma-Aldrich) was used to extract the total RNA following the manufacturers instructions. A total amount of 3 g RNA per sample was used to construct a cDNA library with a fragment length range of 250 to 300 bp by NEBNext UltraTM RNA library Prep Kit following the manufactures recommendation. Then an Illumina Hiseq 2500 platform was used for paired-end sequencing.