title: GSM5417990: RNA-seq AB1 Ts6 rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of WT and trisomic mESCs was isolated following TRIZOL RNA extraction method, and poly(A) based mRNA enrichment was performed. Then mRNA was fragmented and cDNA was synthesized from mRNA library. RNA-seq library was prepared following DNA end repair, dATP tailing, adapter ligation and PCR.