title: Illumina HiSeq 3000 paired end sequencing; RNA-seq of shControl and shRUNX1-ETO Kasumi-1 cells
design: RNA-seq of shControl and shRUNX1-ETO Kasumi-1 cells
library_construction_protocol: Kasumi-1 cells were grown in RPMI1640 supplemented with 20% fetal calf serum until RNA extraction Total RNA was prepared using Trizol  NEBNext rRNA Depletion Kit and NEBNEXT Ultra II Direction RNA Library Prep Kit for Illumina