title: Metagenome of rainwater
design: PCR for detecting DNA and RNA genomes was performed using protocols previously described (Wang et al. 2003; Fernandez-Cassi et al. 2018). PCR products were purified and concentrated to a volume of 50 L using the Agencourt AMPure XP PCR purification kit (Beckman Coulter, CA, USA). DNA libraries were generated using a Nextera XT DNA Preparation Kit (Illumina, San Diego, CA, USA). Paired-end sequencing (2 x 150 bp) was performed on the NextSeq platform (Illumina, San Diego, CA, USA) at SENAI CETIQTs Facility (SENAI Innovation Institute for Biosynthetics, Technology Center and Textile Industry, Rio de Janeiro, RJ, Brazil).