title: GSM5093515: D7 ES Control RNA-seq, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol, depleted of ribosomal RNA and then treated with DNase. The rRNA-depleted RNA was selected by size greater than 200nt, fragmented to a mediam size of about 200nt and then subjected to RT reaction. The subsequent first-strand cDNA was subjected to second strand cDNA synthesis using NEBNext Ultra Directional RNA Second Strand Synthesis Module (NEB, #E7550S).  Double-stranded DNA was subjected to library construction using  NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S) for Illumina.