title: COLON
design: The small and large intestinal contents of MAT PE mice were collected at day 14 after MAT PE. Bacterial DNA was extracted using the E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to manufacturers instructions. The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R(5'-GGACTACHVGGGTWTCTAAT-3'). 16S rRNA gene amplicons were generated and sequenced on Illumina MiSeq PE300 platform according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).