title: Illumina HiSeq 1500 sequencing; Impact of RNA isolation methods for RNA-seq on gene expression. Here RNA was isolated from human peripheral blood mono nuclear cells (PBMCs) using either Columns or magnetic beads and the isolated RNA was processed using prime-seq
design: Impact of RNA isolation methods for RNA-seq on gene expression. Here RNA was isolated from human peripheral blood mono nuclear cells (PBMCs) using either Columns or magnetic beads and the isolated RNA was processed using prime-seq
library_construction_protocol: frozen PBMCs were gently thawed according to standard protocols, cells were counted using a hemicytometer and lysed in RLTplus lysis buffer with 1% beta mercapto ethanol at 4000 cells per 50Âµl RNA extraction using the Zymo Direct-zol RNA MicroPrep Kit The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66