title: Timepoint 2, Animal C, duplicate b
design: A modified version of the guanidium thiocyanate protocol was used for total DNA extraction. Then, samples were purified with SeraMag Carboxylate Modified beads and evaluated by NGS sequencing of V3V4 16s rRNA region. Each sample and sequencing runs were carried out on MySeq Illumina sequencing platform. The Resulting sequencing data was analysed by Qiime2 pipeline for taxonomic classification of 16s sequencing metagenomic data.