title: GSM5684404: human cavernous hemangioma scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: The single-cell suspensions were filtered with a 40-μm-diameter cell strainer (FALCON, USA), then washed twice times with RPMI1640 wash buffer at 4 temperature. Cell viability was determined by trypan blue staining with TC20 automated cell counter (BioRad, USA). The ratio of viable cells in single-cell suspension was more than 85%. The concentration of single-cell suspension was adjusted to 700–1200 cells/μL. The cells of each sample were then processed to generate 10x libraries with the Chromium Single Cell 3' Reagent Kits v3 CG000183 (10x Genomics, USA) following the manufacturer's instruction.