title: NextSeq 550 sequencing; Bulk RNA-seq of human induced pluripotent stem cells (hIPSC) and neural progenitor cells (NPC) differentiated usind Dual SMAD inhibition using the prime-seq method
design: Bulk RNA-seq of human induced pluripotent stem cells (hIPSC) and neural progenitor cells (NPC) differentiated usind Dual SMAD inhibition using the prime-seq method
library_construction_protocol: Cells were cultured and differentiated into NPCs as described previously (Geuder et. al. 2021). One colony per sample was harvested and lysed in 100 µl RLT plus lysis buffer containing 1% beta-mercapto Ethanol and frozen at -80 °C. RNA extraction using carboxyl coated beads as described in the prime-seq protocol The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66