title: GSM5583550: RNA Extracted from FRCs treated with control conditioned media , replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated for each sample using Rneasy micro kit (QIAGEN) according to the manufacturer instructions libraries were processed using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB #E7490)  as recommended by the manufacturer ,Poly A mRNA was isolated from 500 ng of total RNA by means of NEBNext Poly(A) mRNA Magnetic Isolation Module. Subsequently, the isolated poly mRNA was fragmented with the help of temperature and the preparation continued following the recommendation of the manufacturer. The quantity and the quality of the libraries were assessed using Bioanalyzer High Sensitivity DNA Analysis. Finally, The libraries were sequenced using Illumina NextSeq500