title: GSM5098141: ATAC-seq_BT549_rep2; Homo sapiens; ATAC-seq
library_construction_protocol: (ATAC-seq) Nuclei were incubated in fresh 25ul TD Buffer (10mM Tris-HCl, 5mM MgCl2, 10% dimethylformamide, pH8.0) with 2.5ul Tn5 Transposase for 30min at 37°C. QIAquick PCR purification kit(Qiagen; cat. no. 28106) was used to purify DNA fragments. We used HiFi HotStart ReadyMix (KAPA; KK2601) for library amplification following provided manual except number of PCR cycle. The amplified library was purified with a QIAquick PCR purification kit (Qiagen; cat. no. 28106)