title: GSM4823224: iDN3 turnover RP1140A all; Mus musculus; RNA-Seq
library_construction_protocol: Thymi were prepared by gently smashing and passing through a 40uM filter to prepare single cell suspensions. DN3 and CD4 CD8 double-positive thymocytes were purified by FACS sort into Qiagen RLT lysis buffer. RNA was separated from genomic DNA using a modified oligo-dT bead-based method. Libraries were prepared following the Pico Nextera protocol.