title: GSM5897095: LC25_N; Homo sapiens; miRNA-Seq
library_construction_protocol: The patients with untreated, primary, non-metastatic lung tumors, who underwent lung lobe resection with curative intent and provided informed consent, were included in this study. Following surgical resection, paired tumor and normal tissues were isolated and immediately transported to the research facility. The tumor and normal tissues were macroscopically examined for tumor positioning. Ten paired normal and cancer samples from lung adenocarcinoma patients were placed in RNAlater solution (Thermo Scientific, Cat.#AM7020) at 4°C within a few minutes of collection and left overnight to ensure RNA sefety. For further analysis, samples were stored at −20 °C with the RNAlater removed. The integrity and amount of RNA was measured using the Agilent Bioanalyzer 2100. Approximately 1 μg total RNA was used to prepare a small RNA library, according to the protocol of the TruSeq Small RNA RNA library prep kit (Illumina, San Diego, CA). The libraries were quantified using KAPA Library Quantification kits for Illumina sequencing platforms, according to the qPCR Quantification Protocol Guide (KAPA BIOSYSTEMS, #KK4854), and were sequenced (single-end; 51 bp) using the Illumina HiSeq 2500 system (LC Sciences, Houston, TX) by the Macrogen Inc (Seoul).