title: GSM5098453: Mild CTV month 8-D9 [MI8D9]; Citrus sinensis; RNA-Seq
library_construction_protocol: Total RNA was extracted from <100 mg of ground frozen leaf tissue using the Qiagen RNeasy Plant Mini Kit and treated with RNase-free DNase (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and then cleaned using a Zymo Research Clean and Concentrator kit (Zymo Research, Irvine, CA, USA) according to the manufacturer's instructions. RNA-seq libraries were generated from 400 ng of total RNA each, after poly-A enrichment, using the Kapa mRNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) following manufacturer instructions.