title: GSM5100025: G1812_DNaseseq_DHS_rep2; Triticum urartu; DNase-Hypersensitivity
library_construction_protocol: 2.2 μg DNA extracted from the harvested seedlings were used to prepare ChIP-seq, DNase-seq and Bisulphite-seq. RNA was extracted using TRIzol and 2μg total RNA were used to prepare RNA-seq. Nuclei were extracted with H1B buffer(20 mM Tris-HCl, pH 8.0, 50 mM EDTA, 5 mM spermidine, 0.15 mM spermine, 40% glycerol, and 0.1% mercaptoethanol) for DNase-seq. Libraries were prepared for sequencing using standard Illumina protocols.