title: GSM5822777: T1: 5-FU tratemt HCT-8 rep1; Homo sapiens; RNA-Seq
library_construction_protocol: The control and 5-FU treated HCT-8 cancer cells were lysed with TRIzol reagent (Invitrogen, USA). A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.