title: GSM5697119: Satellite cells Replicate C; Bos taurus; RNA-Seq
library_construction_protocol: Cell lysates were harvested by adding TRK lysis buffer (Omega Bio-tek) to tissue culture samples after removing culture media and washing with PBS. RNA was purified using the Omega MicroElute Total RNA Kit (Omega Bio-tek).  Sequencing libraries were prepared from harvested RNA samples using the TruSeq stranded mRNA kit (Illumina) and sequenced on a high-output 75bp NextSeq 500 (Illumina)