title: GSM5229599: CO25_Treg_2_S9; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed after co-cultured and harvested using Trizol reagent. RNA was isolated with Direct-Zol RNA MicroPrep w/Zymo-Spin columns. RNA-seq was performed using the QIAGEN Human Inflammation and Immunity Transcriptome RNA targeted panel (QIAGEN) RNA libraries were prepared for sequencing using standard QIAgen protocols