title: GSM5100232: GFP_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed with PBS, and RNA was harvested using the Qiagen RNeasy Mini Kit with on-column DNase I digestion. Samples were sent to the Institut de Recherches Cliniques de Montréal (IRCM) for library preparation and deep sequencing. RNA integrity was first assessed on Bioanalyzer RNA Pico chips (Agilent), then strand-specific, barcoded libraries were prepared via cDNA production, PCR, and ribosomal RNA (rRNA) depletion . Deep sequencing was performed using the Illumina HiSeq 4000 platform with paired-end 50 reads at approximately 50 million reads per sample.