title: GSM6032518: Polysome fractions – siRNAG5 treatment – Rep1; Homo sapiens; OTHER
library_construction_protocol: RNA from cytoplasmic lysates and polysomes was extracted using phenol-chloroform, isopropanol precipitated, and resuspended in RNase-free H2O. RNA samples were cleaned with the RNeasy kit (Qiagen). RNA quality and concentration was determined using Agilent 2100 Bioanalyzer (Agilent Technologies). The cDNA libraries were prepared with 1 µg of RNA using the Illumina TruSeq Stranded mRNA (Macrogen). RNA libraries were prepared for sequencing using standard Illumina protocols