title: GSM6033321: A375 Melanoma siGCDH Batch1 Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Libraries were prepared from total RNA using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina from Lexogen. Barcoded libraries were pooled and single end sequenced (1×75) on the Illumina NextSeq 500 using the High output V2.5 kit (Illumina Inc., San Diego CA). Following transfection for 48 hr, total RNA was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany).