title: GSM6032614: CBS-/-CSE-/-(2) BE2-4; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Three biological replicates of 5-day-old naive males were collected for each transcriptome analysis. For each replicate, total RNA was isolated from approximately 100 heads of naïve males collected by decapitation of Drosophila male on an ice-cooled surface. The extraction was made using RNAzol RT (Molecular Research Center, USA) according to the company's protocol. The concentration and quality of RNA was determined via Qubit Fluorometer (Invitrogen) and an Agilent BioAnalyzer 2100 respectively using RNA 6000 nano kit. For each of three biological replicates taken for libraries 100 heads were collected. For libraries RNA with Integrity Number (RIN) no less than 8 was taken. Illumina NEB Next Ultra II Directional RNA Library Prep Kit (NEB, USA) was used for mRNA libraries preparation. The sequencing was performed on the Illumina NextSeq 500 platform. For each library sequencing provided around 15-20 million reads.