title: GSM5099833: ENDOC-βH1 cells non treated rep 1 -ribosome profiling [EndoNT]; Homo sapiens; OTHER
library_construction_protocol: Lysates for ribosome profiling libraries were prepared following Ingolia et.al protocol. For long-RNA sequencing, RNA isolation was performed using Trizol extraction. For generating ribosome profiling libraries Ingolia et.al protocol was followed. Libraries for the direct RNA sequencing were generated using the recommended protocol for the SQK-RNA001 kit (Oxford Nanopore Technologies). For ribosome profiling Illumina next generation sequencing was performed. For long-RNA sequencing, libraried were loaded on MinION R9.4 flow cells (Oxford Nanopore Technologies) for 48 hours.