title: GSM6032592: 5 mg EE2/kg body weight, rep5 [QA1-H6]; Coturnix japonica; RNA-Seq
library_construction_protocol: Liver tissue was homogenized in buffer RLT using a TissueLyser II (Qiagen, Toronto, ON, CA), and RNA was extracted using the RNeasy® Mini QIAcube Kit (Qiagen) following the manufacturer's protocol. An extra DNase I digestion step was included to eliminate genomic DNA contamination. RNA libraries were prepared for sequencing using standard Illumina protocols