title: GSM6167132: Liver DDC neutrophil sample 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using the commercial kit miReasy Micro Kit (Qiagen) following manufacturer's instructions. The quantity and quality of the RNAs were evaluated using Nanodrop and Agilent RNA 6000 Pico Chips (Agilent Technologies, Cat.# 5067-1513). Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat.# 634411), following “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian User Manual (Rev. 063017)”. Paired-ended sequencing was performed on Illumina platform.