title: GSM4879953: CHBP+ M-MDSC; Mus musculus; RNA-Seq
library_construction_protocol: The Raw Reads obtained by sequencing may contain unqualified Reads with low overall quality, sequencing primers, low end quality, etc. These unqualified Reads are likely to have a certain impact on the analysis quality, so they must be filtered to obtain Clean Reads that can be used for data analysis, apply Seqtk to filter, the main steps are as follows:  1. Remove the linker sequence contained in reads;  2. Remove the bases whose 3'end quality Q is less than 20, that is, the base error rate is less than 0.01, where Q=-10logerror_ratio;  3. Remove reads whose length is less than 25;  4. Remove the ribosome RNA reads of the species. The Raw Reads obtained by sequencing may contain unqualified Reads with low overall quality, sequencing primers, low end quality, etc. These unqualified Reads are likely to have a certain impact on the analysis quality, so they must be filtered to obtain Clean Reads that can be used for data analysis, apply Seqtk to filter, the main steps are as follows:  1. Remove the linker sequence contained in reads;  2. Remove the bases whose 3'end quality Q is less than 20, that is, the base error rate is less than 0.01, where Q=-10logerror_ratio;  3. Remove reads whose length is less than 25;  4. Remove the ribosome RNA reads of the species.