title: GSM6034412: IL33, KO, Astrocytes (4OTR.TAGGCATG_CTAGTCGA); Mus musculus; RNA-Seq
library_construction_protocol: Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on a negative selection strategy for astrocytes. Libraries were prepared by the Broad Institute according to the SMARTseq 2 protocol and 10X Single Cell V3 as described (Trombetta et al 2014).