title: GSM6033802: Naïve Blood 2; Mus musculus; RNA-Seq
library_construction_protocol: Blood neutrophil isolation: Mice were euthanized by isoflurane overdose. Blood was collected from the left ventricle in an EDTA tube. PBMC were isolated over a lympholyte gradient (Cedarlane labs), and incidental red blood cells were lysed using an Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Quality Biological).    Neutrophil isolation: Neutrophils were isolated from the i.p. lavaged cells with MACS Ly6G magnetic beads (Miltenyi Biotec) following the manufacturer's instructions. Purity (95-99%) was verified using flow cytometry. The cDNA libraries were constructed using the 10x Chromium Single cell 3′ Library Kit according to the manufacturer's protocol. For quality control after library construction, 1 μl of sample was diluted 1:10 and ran on the Agilent Bioanalyzer High Sensitivity chip for qualitative analysis. For quantification, an Illumina Library Quantification Kit was used.