title: GSM5418890: AHR KO_Stem cells_5nMFICZ-1; Mus musculus; RNA-Seq
library_construction_protocol: Colon crypts were isloated with 2mM EDTA wash. Crypts embedded in matrigel were cultured in stem cell enrichment media containing Wnt-3A, Noggin, Rspondin-1 and EGF. RNA was extracted from organoids and quantified with Bioanalyzer RNA libraries were prepared for sequencing using standard Illumina protocols