title: GSM5697073: S11G2T2435; Homo sapiens; RNA-Seq
library_construction_protocol: PDL cells were washed with cold PBS after gentle scrapping from the extracted premolar. Total RNA was extracted from PDL cells using TRIzol Reagent. The RNA was stored at −70 °C and measured at an optical density of 260 nm. The mixtures of total RNA were incubated with Oligo dT. Real-time PCR was performed for the selected upregulated and downregulated genes to verify RNA-seq results. The library was constructed, sequenced using an Illumina HiSeq2500 sequencer, and the data obtained through RNA-seq.