title: GSM5288368: MOLM13_sgSCP4_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol following the manufacture's protocol RNA-seq library was prepared using Illumina TruSeq sample prep kit v2 according to the manufacture's protocol. Briefly, polyA RNA was selected and fragmented enzymatically. First strand of cDNA was synthesized using Super Script II reverse transcriptase, and then second strand was synthesized. Double-stranded cDNA was end-repaired, 3'-adenylated, ligated with indexed adaptor, and then PCR-amplified.