title: GSM6032884: Virion_1; Murine hepatitis virus; RNA-Seq
library_construction_protocol: Total RNA was isolated with the Trizol method 1 µg of total RNA isolated from MHV was used for each sample. Libraries for direct RNA-seq were prepared using the Direct RNA Sequencing Kit (SQK-RNA002, Oxford Nanopore Technologies). All the libraries were multiplexed using the DeePlexiCon adapters, loaded to Nanopore R9 Flow Cells (FLO-MIN106D, Oxford Nanopore Technologies) and run on a MinION sequencer.