title: GSM6033754: HepG2_caffeine 1 μM_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: After the culture medium with caffeine was removed, the cells were harvested by digestion of trypsin-EDTA solution and washed with ice-cold PBS twice and then centrifuged at 12,000 g for 15 min at 4 °C. RNAs were then purified by Trizol reagent. RNAs were quantified by a NanoPhotometer. The RNA samples were purified by poly (dT) beads, converted to cDNA using SmartScribe Rtase kit according to manufacturer's protocol.