title: GSM5822763: Cirrhotic_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the HCC tissues using TRIzol reagent according to the manufacturer's instructions. RNA degradation and contamination were monitored on 1% agarose gels. RNA concentration and purity were assessed using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). A total amount of 5 μg high-quality RNA per sample was used as input material for the RNA sample preparations. Ribosomal and linear RNA were removed by Epicentre RibozeroTM rRNA Removal Kit (Epicentre, USA) and RNase R (Epicentre, USA) respectively.Subsequently, circRNA was enriched by using acid phenol/ chloroform (pH 4.5). The sequencing libraries were prepared by NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.