title: Illumina HiSeq 4000 sequencing; 3'end RNA sequencing of TDP-43 depleted and mutant TDP-43-rescued Hek-293 FlpIn lines
library_construction_protocol: Cells collected by scraping of from TC plates DMEM siRNA and siRNA with Doxycycline induced rescue RNA was extracted from cell pellets using Maxwell RSC simply RNA cells kit in Maxwell RSC instrument following manufacturerâ€™s instructions.  QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina