title: GSM6276605: C57BL/6J mice, Brown adipose tissue,control [ND4]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the BAT using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) , and was used for the construction of sequencing libraries. Bowtie2 (v2.2.5)   was applied to align the clean reads to the gene set, a database built by BGI (Beijing  Genomic Institute in ShenZhen), in which known and novel, coding and noncoding  transcripts were included, then expression level of gene was calculated by RSEM  (v1.2.12) .