title: GSM6033295: E6_FGO_WT_paiso; Mus musculus; OTHER
library_construction_protocol: To obtain oocytes or preimplantation embryos, 8-weeks-old or 5-weeks-old C57BL/6 female mice were intraperitoneally injected with pregnant mare's serum gonadotropin (PMSG; 5 IU) and human chorionic gonadotrophin (hCG; 5 IU). Preimplantation embryos were collected from 5-weeks-old C57BL/43 female mice mated with PWK/PhJ male mice. Total RNA was extracted by TRIzol (life tech; 15596018) and further purified through isopropanal precipitation and resolved in 11 μl nuclease-free water. Total RNA is used for end extension with dU-containing end extension oligos by Klenow fragment (3'→ 5' exo-, NEB; M0212L). The end-extended RNAs (>200 nt) was cleaned up by RNA Clean & Concentrator-5 kit (Zymo; R1015) and eluted by 7 μl nuclease-free water. The cleaned RNA was reverse transcribed by SuperScript II similar to Smart-seq2 method. The amplified DNA was size selected by Ampure beads and made into SMRTbell Template library. The libraries were sequenced by Pacbio sequel II.