title: GSM5517376: C4-2B  Vehicle; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq libraries were from 1 μg total RNA which was extracted using the total RNA isolation kit (Tiangen, BJ, China) and reverse transcribed with reverse transcriptase M-MLV (Takara, DL, China), according to the manufacturer's instructions. Libraries were validated with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Sequencing was performed on an MGISEQ2000 SE50 sequencer at BGI Tech (Wu Han, China). RNA libraries were prepared for sequencing using standard Illumina protocols