title: GSM5290766: R2i_BMPi_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the R2i cultured mESCs and BMP inhibitor treated R2i cultured mESCs (3 biological replicates per each group) using Qiagen RNeasy mini Kit. mRNA was then isolated from the total RNA and fragmented using Illumina Truseq™ Stranded mRNA LT Sample Prep Kit with poly-T oligo magnetic beads, and reverse transcription of the mRNA fragments was conducted using Superscript III reverse transcriptase (Life Technologies). Adaptor ligated libraries were generated according to the manufacture's standard protocol and sequenced using HiSeq 2500 system.