title: GSM5584132: PKM2_IP_1; Homo sapiens; RIP-Seq
library_construction_protocol: The beads were washed 6 times with lysis buffer and then divided into two groups, one for RNA isolation from PKM2-RNA complexes and another for the western blotting assay for PKM2 immunoprecipitation.The PKM2-bound RNAs were isolated from the immunoprecipitate of monoclonal Flag antibody (Sigma), followed by the preparation of the Illumina Truseq pair-end libraries. The reverse transcription was performed with RT primers harboring 3′ adaptor sequence and random hexamer. The generated cDNAs were PCR-amplified and the 200–500 bp products were purified