title: GSM5517745: Btn1a1 WT rep4; Mus musculus; RNA-Seq
library_construction_protocol: Lactating mammary glands were removed, homogenised and flash frozen on dry ice in Trizol reagent (Zymo Research Corp). mRNA was fractionated from total RNA (2 mg/sample) using an Illumina TruSeq mRNA library preparation kit.  The mRNA was then fragmented and coverted to double-stranded cDNA and amplified by PCR to generate libraries for pair-end sequencing..