title: GSM5782185: Ghurrah Post-challenge MDM at 48 hours post infection (DI2-3); Sus scrofa; RNA-Seq
library_construction_protocol: At 48 hpi, the MDMs were harvested from the culture plates. RNA isolation from MDMs was performed using TRIzol method. The quantification of viral load in MDMs of two groups (Ghurrah and Landrace) was undertaken via absolute quantification with qPCR using primers that were specific to 5'UTR. Library preparation was done through Illumina Truseq RNA sample preparation kit V2 (PolyA capture).