title: GSM5229402: Total1_SmallRNA; Gallus gallus; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from the midbrain region of HH9- chick embryo heads. After RNA extraction, small RNA was size selected by acrylamide gel purification and half of each sample was oxidized with sodium periodate to select for 2'O-methylated species (piRNAs). RNA libraries were prepared for sequencing using standard Illumina protocols for small RNA cloning