title: GSM6032623: skeletal muscle sample 1; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen RNeasy Mini Fibrous Tissue Kit was used to isolate RNA from 10 mg of frozen muscle tissue samples. Samples were homogenized using the TissueLyser II system with shaking twice for 2 min at 25 Hz. Total RNA samples were treated with DNase I using Turbo DNA-free Kit (Thermo Fisher Scientific) according to the kit guidelines. Only RNA samples with RIN>7 were included in the study. Samples were stored at -80 C until sequencing libraries were prepared. Libraries for RNA sequencing were prepared from 100-300 ng of total RNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBNext rRNA Depletion Module (New England Biolabs). RNA libraries were sequenced on the Illumina HiSeq system with 250 cycles.