title: GSM5100985: Plate_Q8_115; Mus musculus; RNA-Seq
library_construction_protocol: The dissected tissue sections were then subject to protease digestion, after which cells were dissociated and hand picked into PCR strip tubes containing 3 μl lysis buffer (0.2% TritonX-100 (Sigma) and 0.1 U/μl RNase inhibitor (Lucigen)) and cDNA libraries were prepared using the Smart-SCRB chemistry as described previously (Cembrowski et al., 2018; Xu et al., 2020). 600 pg cDNA from each pooled sample was used in a modified Nextera XT library preparation (Illumina) (Soumillon et al., 2014) using the P5NEXTPT5 primer and extending the tagmentation time to 15 m. The resulting libraries were purified according to the Nextera XT protocol (0.6x bead ratio) and quantified by qPCR using Kapa Library Quantification (Kapa Biosystems).