title: GSM5293033: Sample-66 (Total Bulk RNA-seq); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolation from frozen brain samples using Mini Nucleospin RNA kit, Takarabio About 500ng total RNA was used to prepare the cDNA library using SMARTer Stranded Total RNA Sample Prep kit-HI Mammalian For snRNA-seq and snATAC-seq, single nucleus suspensions were isolated from about 50mg frozen human prefrontal cortex following 10x genomics recommendation. For bulk RNA-seq, total RNA was isolated using Mini Nucleospin RNA kit, Takarabio Libraries were prepared using 10x genomics Chromium Single Cell ATAC platform (V1), 10x genomics chromium V3 for snRNA-seq and SMARTer Stranded Total RNA Sample Prep kit-HI Mammalian  for bulk RNA-seq