title: GSM5100243: DNA from seed produced at 25°C_Mature stage, T26_M1; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Total DNA were extracted from the frozen grinded tissues lots using the Macherey-Nagel NucleoSpin® DNA Food kit following manufacturer's instructions DNA samples were sent to to the BGI and library construction, bisulfite treatment using a ZYMO EZ DNA Methylation-Gold kit and paired-end sequencing using an Illumina Hiseq 2500 (PE100 20M) Library Construction (MGIEasy Whole Genome Bisulfite Sequencing Library Prep Kit) details:  Fragment genome DNA to 100-300 bp by Sonication  DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptors.  1) Bisulfite treatment by ZYMO EZ DNA Methylation-Gold kit. (Unmethylated Lambda DNA, Promega as control)    2) PCR amplification and purification    3) Denaturation    4) ssDNA circularization    5) Enzymatic Digestion and purification    6) Qualified library for sequencing.