title: PromethION sequencing
library_construction_protocol: DNA from the hybrid S. lycopersicum cv. Funtelle was extracted using the Machery Nagel Nucleobond HMW DNA Kit. High molecular weight DNA was size selected using the Circulomics Short-Read Eliminator XL Kit (Circulomics Cat# SKU SS-100-111-01) and used as input for a library preparation with the Oxford Nanopore LSK-110 ligation sequencing Kit. Four libraries from the same sample were loaded onto three PromethION PRO-002 Flowcells and one MinION Flowcell (all R9.4.1 pores) and sequenced. ONT Sequencing was performed on an Oxford Nanopore P24 PromethION and a MinION MK1B at the Forschungszentrum JÃ¼lich using MinKNOW version 21.02.7 without live basecalling. Basecalling of the Oxford Nanopore read data was done using guppy basecaller version 5.0.11 using the R9.4.1 PromethION super-high-accuracy model (dna_r9.4.1_450bps_sup_prom) and the R9.4.1 MinION super-high-accuracy model (dna_r9.4.1_450bps_sup).