title: GSM5395599: M; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from peripheral blood with the Quick DNA/RNA miniprep plus kit (ZymoResearch) according to the manufacturer's instruction. The RNA purity was evaluated with NanoDrop Spectrophotometer 2000 (Thermo Fisher Scientific) and RNA integrity number (RIN) was determined with Agilent 2100 Bioanalyzer (Agilent Technologies).  Alpha and beta globin were depleted from total RNA with the GLOBINclear Kit (Invitrogen by Thermo Fisher Scientific) and sequin synthetic RNA spike-in controls were added to mRNA as sequencing internal contro Libraries were produced with QuantSeq 3' mRNA-Seq Library Prep Kit for Ion Torrent (Lexogen), according to the manufacturer's instruction.