title: GSM6033338: SKBR3 Breast siControl Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA).  Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA). Following transfection for 48 hr, total RNA was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany).