title: NextSeq 500 sequencing; ZXDA/B dependent transcription in HCT116 human colon carcinoma cells
library_construction_protocol: Cells were collected 48 hours after transfection and lysed in RLT buffer. HCT116 cell line was transfected (RNAiMAX) using two different control and ZXDA/B targeting siRNA. RNA was extracted using QIAGEN Rneasy plus kit. Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit.