title: GSM5049426: Kelly Neuroblastoma cells rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-sequencing was performed on the following human NB cell lines: Kelly, NBL-S, CHP-212, SH-SY5Y, SH-SY5Y LDK-resistant and SH-EP. Biological duplicates (5 x 106 cells per replicate) were homogenized in 1 ml of TRIzol Reagent (Invitrogen) and purified using the mirVANA miRNA isolation kit (Ambion) following the manufacturer's instructions. Total RNA was treated with DNA-freeTM DNase I (Ambion), spiked-in with ERCC RNA Spike-In Mix (Ambion) and analyzed on an Agilent 2100 Bioanalyzer (Agilent Technologies) for integrity. Sequencing libraries were prepared using LP-KAPA mRNA Hyper Prep and sequenced using Illumina HiSeq for 40 bases.