title: GSM6166507: smRNA suvh2suvh9_sdlg_2; Arabidopsis thaliana; miRNA-Seq
library_construction_protocol: For meiocyte, 1 U/μL Recombinant RNase Inhibitor (Takara, 2313A) was added to the collection buffer. 300-400 meiocyte masses were used to extract ~400-1000 ng RNA for a biological replicate. The RNA was extracted using Trizol (ThermoFisher, 15596026). For seedling, 4-5 plants were ground in liquid nitrogen and the total RNA was also extracted using Trizol. Small RNA libraries were constructed following the TruSeq small RNA library prep reference guide (Illumina)