title: GSM4879717: WT mESCs - RCOR2 ChIP-seq rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Approximately 30 x 10^6 cells were used per ChIP. The samples were pre-cleared with 100 μL of Pierce Control Agarose Resin (Thermo Fisher Scientific) for 1 h  at 4 °C and immunoprecipitations were performed with 30 μL of Pierce Anti-HA Agarose (Thermo Fisher Scientific) per sample rotating overnight at 4 °C. Libraries were prepared using the ThruPLEX DNA-seq 12S kit (Rubicon Genomics, Cat#R400429). After limited PCR amplification, libraries were purified using Agencourt AMPure XP beads (Beckman Coulter, Cat#A63880), according to the manufacturer's recommendations. 75 bp paired end sequencing was performed on an Illumina HiSeq 3000.