title: GSM5229848: DMSO-treated jejunal organoids, rep3 [DMSO_Jour2_N3]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using Rneasy mini kit (Qiagen) Libraries were generated from 250 ng of total RNA as follows: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs, Whitby, Canada). cDNA synthesis was achieved using NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Module (New England BioLabs). The remaining steps of library preparation were performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs).