title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of human donors with severe type 1 interferonopathy due to germline STAT2 gain-of-function
library_construction_protocol: Whole blood was collected into PAXgene tubes (Qiagen, Valencia, CA). Written informed consent for the use of patient material was provided. Ethical approval was granted by the NRES Committee Leeds (East) (Ref: 07/Q1206/7). Samples were collected and RNAseq undertaken. ISG score was calculated as described in PMID: 24183309. The genotypes leading to interferonopathy were assigned according to PMID: 30633609. Total RNA extracted using a PreAnalytix RNA isolation kit. RNA integrity was analysed on an Agilent 2100 Bioanalyzer.  Truseq stranded mRNA Assay. Paired end.