title: GSM5005813: AB5075 wildtype; Acinetobacter baumannii; RNA-Seq
library_construction_protocol: Total bacterial RNA was isolated utilizing an RNeasy Kit (Qiagen) and contaminating DNA was removed by treatment with TURBO DNA-free kit (Ambion). Samples with a RIN of >9.9 measured by Agilent 2100 Bioanalyzer system and corresponding RNA 6000 Nano kit (Agilent) were used in this study. Ribosomal RNA was removed using a Ribo-Zero Kit for Gram-negative Bacteria (Illumina) and MICROBExpress Bacterial mRNA enrichment kit (Agilent). Library construction was performed using the TruSeq Stranded mRNA Kit from Illumina omitting mRNA enrichment steps.