title: GSM5229664: OsNPR1-OE/slr1-1-R3; Oryza sativa Japonica Group; RNA-Seq
library_construction_protocol: Total RNA was extrated using TRIzol method. 1.Total RNA used DNaseI to digest DNA, and enriched mRNA using magnetic beads with Oligo(dT).  2.Adding reagent in Thermomixer to interrupt mRNA into short fragments.  3.The fragmented mRNA was used as template to synthesize the first strand of cDNA, and then synthesized the second strand.  4.Purified, repaired, added "A" and adapters in the DNA, then selected 200-300bp fragments and performed PCR amplification.   5.The libraries were checked with Agilent 2100 Bioanalyzer and ABI Real-Time PCR System, the qualified libraries were sequenced using sequencing platform.