title: GSM5823886: eif4a3-RPF-KD, rep2; Mus musculus; OTHER
library_construction_protocol: Ribosome profiling was performed according to the manufacturer's instructions. Total RNA and RPF libraries were prepared using the TruSeq Ribo Profile (Mammalian) Kit (Illumina, RPHMR12126) according to the manufacturer's reference guide (document #15066016 v01) with Ribo-Zero Gold Kit (H/M/R) (Illumina, MRZG126). Two biological replicates were performed for total RNA and RPF RNA of eIF4a3 shRNA-infected ESCs. Massively parallel sequencing was performed with the Illumina HiSeq2500 according to the manufactory protocol, and single-end 50 bp-length reads were produced. For RPF samples, each sample was sequenced four times  to get enough reads.