title: GSM5099551: Microwell Round_Day10_Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: For total RNA extraction the following samples were collected from three independent culture plates: 10 pooled human cerebral organids (hCO) for day 10 samples, 6 pooled hCOs for day 20 samples, and 4 pooled hCOs for day 40 samples. hCOs were washed 3 times in cold PBS. Matrigel was dissolved by incubating the hCOs in chilled Cell Recovery Solution (Corning, cat. no. 354253) for 1h at 4 °C. The dissolved Matrigel was removed by rinsing 3 times in cold PBS. Total RNA was isolated using Direct-zol RNA MicroPrep Kit (Zymo Research) according to the manufacturer's protocol. RNA samples were collected in 2mL RNAse-free tubes and chilled on ice throughout the procedure. RNA libraries were prepared and sequenced by BGI using DNB-seq PE100 platform.