title: GSM6032669: MCN_water deprived_72 hours_Input; Rattus norvegicus; ChIP-Seq
library_construction_protocol: ChIP was performed using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, 38191). Samples were disrupted for 15 seconds with a Biomasher homogeniser (Takara Bio). To crosslink proteins to the DNA, formaldehyde (Sigma-Aldrich, F8775) was added to each sample to a final concentration of 1.6% followed by end over end mixing (Tube Rotor, VWR, 444-0502) for 20 minutes at room temperature (RT). Crosslinking was stopped by adding 50 µl of 10x glycine solution per 500 µl sample followed by end over end mixing for 5 minutes at RT. ChIP-seq DNA libraries were prepared from 1 ng of ChIP DNA and 1 ng of Input DNA using a proprietary library protocol from the EpiQuest service by Zymo Research.