title: GSM5098351: Peripheral naïve OTII CD4 T cells [PO_2]; Mus musculus; RNA-Seq
library_construction_protocol: CD25neg Va2+ CD4SP thymocytes or peripheral naïve (CD44low CD62L+) CD25neg Va2+ CD4 T cells were sorted from OTII (n=3 mice), W131AOTII (n=12 mice, 4 mice/group), Cbl-b−/− (n=3 mice), or Cbl-b−/−W131AOTII (n=6 mice, 2 mice/group) and washed with PBS. Total RNA was isolated from 106 cells per sample using RNeasy (Qiagen) following the manufacturer's protocol. Libraries were prepared using the Nugen/Nextera XT kit (Illumina) and single-end 50pb RNA sequencing was performed using a HiSeq 4000 (Illumina).