title: GSM6033254: AX-REG1-RESUB; Ambystoma mexicanum; RNA-Seq
library_construction_protocol: The retinas were flash-frozen in liquid nitrogen, stored in -80 C until use, and shipped to a Genewiz sequencing facility on dry ice for total RNA extraction and sequencing. The uninjured retinas were pooled into 3 samples (4 retinas per sample, as axolotl retinas are very small). The regenerating retinas were also pooled into 3 samples, with the same animals making up each sample. RNA libraries were prepared for sequencing using using the NEBNext Ultra II RNA Library Prep Kit for Illumina