title: GSM5100314: Caco2 infected with Encephalitizoon intestinalis [EI-2_S6]; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation was conducted using RNeasy Mini Kit (Qiagen, Catalog No. 74104) according to the manufacturer's instructions. The quality of RNA was first checked using Agilent 2200 TapeStation System (Agilent Technologies, Santa Clara, CA). All the RNA samples had an RNA integrity number (RIN) of >9.0 and were used for subsequent processing. The maximum recommended concentration was used as an input and they were subjected to oligo(dT)-based mRNA isolation using an NEBNext poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA). Illumina compatible RNAseq libraries were then prepared  using an NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Ipswich, MA). AMPureXP beads (Beckman Coulter, Brea, CA) were used to purify cDNA libraries. The quality and the size distribution of the libraries were determined by TapeStation and were  quantified using Qubit Fluorometer (Invitrogen). Barcoded libraries were pooled at equimolar ratios and sequenced on Illumina NextSeq 500 platform (Illumina, San Diego,CA) with 1×75 configuration.