title: GSM5782090: Col1a2-Foxf2-TRAMP prostate stroma; Mus musculus; RNA-Seq
library_construction_protocol: Prostate tissues were incubated in DMEM/Collagenase/Hyaluronidase/FBS for 3 hours at 37Â°C, followed by one hour-incubation in 0.25% Trypsin-EDTA on ice. Thereafter, mouse prostate tissues were pelleted, resuspended in Dispase and DNase I, and pipetted vigorously to dissociate cell clumps. Dissociated cells were then passed through 70 Î¼m cell strainers to obtain single cells. Stromal: Lin(CD45CD31CD235a)-Trop2-,  and immune: CD45+, fractions were purified by FACS 10X Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1