title: GSM5100689: Human CD4+ T cells_ki0100022_CMV-_batch4_gene; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood samples were drawn. PBMC were isolated from whole blood by means of density gradient centrifugation. CD4+ T cells were extracted using MACS negative CD4+ T cell isolation kit. Gene expression libraries and feature barcoding libraries were prepared using Chromium Single Cell 3′ V3 Reagent Kits with Cell Surface Protein Feature Barcoding Technology for Cell Surface Protein (10X Genomics). VDJ libraries were prepared using Chromium Single Cell V(D)J V1 Reagent Kits (10X Genomics). The sequencing of gene expression libraries was conducted as a custom configuration (26x8x91bp) on the Illumina HiSeq3000 with a depth of 50,000 reads per targeted cell. The sequencing of VDJ libraries was conducted as PE150 on the Illumina HiSeq3000 with a depth of 5,000 reads per targeted cell. The sequencing of 10X feature barcode libraries was conducted as a custom configuration (26x8x25bp) on the Illumina HiSeq3000 with a depth of 5,000 reads per targeted cell. Junk cells with low read depth were removed and the remaining libraries were sequenced to a depth of 60M to 190M reads per sample in total.