title: GSM5823256: Pooled_cells, HTO; Mus musculus; OTHER
library_construction_protocol: Cells from each group were barcoded with different Biolegend anti-mouse Hastags: CTTTGTCTTTGTGAG:  HT5_Control !Sample_extract_protocol_ch1 = TATGCTGCCACGGTA:  HT6_cMAF_KO GAGTCTGCCAGTATC:  HT7_MAFb_KO TATAGAACGCCAGGC:  HT8_dKO After hashtag staining cells were pooled and approximately 20 000 cells suspended in PBS with 0.04% BSA were loaded into the Chromium Controller, in which they were partitioned, their polyA RNAs captured and barcoded using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics). The cDNA were amplified and libraries compatible with Illumina sequencers were generated using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics). The libraries were sequenced on an Illumina NovaSeq sequencer on an SP100 cell flow (read1: 28 cycles; index: 8 cycles; read2: 80 cycles) at a depth of 50 000 reads per cell. scRNA-Seq