title: GSM6166412: Term-Seq dPNP_3; Bacillus subtilis; OTHER
library_construction_protocol: Total RNA was extracted from collected cell paste via RNeasy mini-kit (Qiagen, cat # 74104) total RNA was dephosphorylated using CIP (NEB, discontinued), rRNA was depleted via ribo-zero Gram-positive rRNA depletion kits (Illumina, discontinued) or MICROBExpress (Thermo Fisher), remaining RNA was ligated to custom unique 2', 3' dideoxy RNA oligonucleotide (IDT), Illumina sequencing libraries were generated from ligation products using TruSeq Stranded mRNA kit (Illumina, cat # 20020594) or NEBNext Ultra Directional (NEB, cat # E7760L)