title: GSM4780786: 10_HeLa_LUC7L2_WT_Glu; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from LUC7L2 WT and LUC7L2 KO K562 and HeLa cells (N=3, each) were isolated using a RNeasy kit (QIAGEN). RNA sequencing libraries were prepared by the Genomics Platform at the Broad Institute based on the True-Seq protocol (Illumina), which selects for polyadenylated RNA and preserves strand specificity. Libraries were sequenced using a NovaSeq 6000 instrument, generating 2x101bp paired-end reads with a minimum of 40M pairs per sample.