title: GSM5100165: Adult BM HSC rep2; Mus musculus; Bisulfite-Seq
library_construction_protocol: C57BL/6 background mice were breed in Specific Pathogen Free (SPF) condition and mice experiments were approved by the ethics committee of the Laboratory Animal Center of Academy of Military Medical Sciences. E10.0 and E11.0 Embryos were staged by somite pair (sp) counting according to 31–35 sp and 41–45 sp separately. bisulfite conversion was performed on cell lysates using MethylCode Bisulphite Kit (Invitrogen), after bisulphite conversion, the converted templates were conducted by the standard protocol. The final quality-insured libraries were quantified using Qubit ds DNA high sensitivity dye (Invitrogen) and then sequenced on NOVA PE 150 sequencer.