title: GSM5100694: BWF1 Male #3; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were diced and digested with collagenase (Sigma Type VIII) and CD11b+F4/80+, cells were sorted by FACS, RNA was extracted using the Qiagen Rneasy plus mini kit (#74134), and rRNA was depleted using the NEBNext rRNA depletion kit (#E6310) RNA libraries were prepared for sequencing using NEBNext Ultra II RNA Library prep kit (#E7775) and indexed using NEBNext Multiplex Oligos for Illumina (#E7335, E7500)