title: GSM6032674: Sprague Dawley_SON non-targeting shRNA_rep5L; Rattus norvegicus; RNA-Seq
library_construction_protocol: A 1 mm punch was used for tissue collection from frozen cryostat cut sections. QIAzol Lysis Reagent (Qiagen) followed by Direct-zol RNA miniprep extraction kit (Zymo research). 100 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were constructed using TruSeq® Stranded mRNA library preparation kit (20020594, Illumina)