title: GSM5100653: S_pH6_short rep 1; Hordeum vulgare; RNA-Seq
library_construction_protocol: Root meristematic tissue was collected in RNAlater (Invitrogen). mRNA was isolated from root tips with the use of Dynabeads mRNA DIRECT Micro Kit (Thermo Fisher Scientific). The RNA-seq libraries were prepared using the TruSeq Stranded mRNA kit (Illumina) according to the manufacturer's instructions. Indexed libraries were pooled, clustered with use the cBot and sequenced on the Illumina HiSeq 4000 platform.