title: GSM5583904: NPCI; Xenopus laevis; RNA-Seq
library_construction_protocol: Brain regions were collected from stage 46 optic tectum and total RNA was extracted using the mirVana kit (Life Technologies, USA). DNase treatment was used to remove genomic DNA and was followed by clean-up using RNeasy mini kit (Qiagen, USA). 1µg of cDNA was sheared in microTube (Covaris) and then used for library preparation (KAPA Taq PCR kits). The next generation sequencing was done using HiSeq2000 platform (Illumina; RRID:SCR_020132) for single-end reads at size 100bp.