title: GSM5823597: BYSR-2; Burkholderia sp. JP2-270; OTHER
library_construction_protocol: DAP-seq was carried out in duplicate on Burkholderia sp. JP2-270 genomic DNA. Specifically, the Halo-BysR protein was expressed using the TNT SP6 Wheat Germ Protein Expression System (Promega, Fitchburg, WI, USA). The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega) and verified by Western blotting with the anti-HaloTag antibody (Promega). Genomic DNA was extracted from JP2-270 using DNAiso (Takara, Japan). Genomic DNA library was generated using NEXTflex Rapid DNA Seq kit (Bioo Scientific, USA) following the manufacturer's instructions. The purified protein was incubated with DNA library at 30 ° C for 2 h, and then the unbound DNA fragments was washed away. Then, the BysR-bound DNA fragments were recovered and PCR-amplified. The resultant products were sequenced using the Illumina NovaSeq platform with the sequencing strategy of PE150. DAP-seq