title: GSM5396148: MZdnmt3aa_2 dpf_WGBS; Danio rerio; Bisulfite-Seq
library_construction_protocol: Zebrafish wild-type (WT) and MZdnmt3aa-/- embryos at 2 days post-fertilization (dpf) were digested with proteinase K and sodium dodecyl sulfate to extract genomic DNA. DNA samples were prepared by mixing zebrafish genomic DNA and unmethylated lambda DNA (Promega) as spike control, at a ratio of 1000:1. DNA samples (100 ng) were subjected to bisulfite treatment using the EZ DNA Methylation-Gold Kit (Zymo Research). Bisulfite-treated DNA samples (50 ng) were added as adapters in 10 cycles of PCR with random primers in the TruSeq DNA Methylation Kit (Illumina). Libraries with adapters were sequenced on the HiSeq X Five Sequencing System (Illumina) with 150 bp single-end reads. Bisulfite treatment, library preparation, and sequencing were performed by Takara Bio Inc. (Shiga, Japan).