title: GSM5098635: S6_Input_ Rep1; Fragaria vesca; RIP-Seq
library_construction_protocol: Total RNA was extracted with the plant RNA extraction kit (Magen, R4165-02) from woodland strawberry fruit at S6, RS1, and RS3 stages, respectively. mRNA was isolated from the total RNA and then fragmented, followed by immunoprecipitation with specific anti-m6A antibody (Synaptic Systems, 202003). For library construction, 50 ng of immunoprecipitated mRNAs or pre-immunoprecipitated mRNAs (input control) were used for library construction with NEBNext ultra RNA library prepare kit for illumina (NEB, E7530). High-throughput sequencing was performed on the illumina HiSeq X sequencer with a paired-end read length of 150 bp according to the standard protocols.