title: GSM5229757: Gastrocnemius muscle Cry2flox; Mus musculus; RNA-Seq
library_construction_protocol: Gastrocnemius muscle was isolated from CRY2scko mice and CRY2flox/flox mice. Total RNA was extracted using the RNAsimple total RNA Kit per manufacturer's instructions. The quality of the total RNA was inspected by Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA). Qualified total RNA was further purified by RNAClean XP Kit (Cat# A63987, Beckman Coulter, Inc, Brea, CA). The cDNA library was generated using a VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® (Cat# NR604-02, Vazyme, Nanjing, China) and sequenced on an Illumina system (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols