title: GSM5395753: Negative control; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated by RNeasy mini kit (Qiagen, Hilden, Germany), and the quantity of total RNA was measured by using a NanoDrop equipment (Thermo Fisher Scientific, Waltham, MA, USA). Complementary DNA (cDNA) was synthesized using the cDNA Synthesis kit (Takara, Otsu, Japan). Real-time PCR was performed using SYBR Green PCR Master Mix (Takara) in a StepOnePlus RT-PCR system (Thermo Fisher Scientific). All target genes were normalized to the endogenous reference gene β-actin by using an optimized comparative Ct (2ΔΔCt) value method. First strand cDNAs were transcribed to the 5' end of capped RNAs, attached to CAGE "bar code" tags.