title: Illumina NovaSeq 6000 paired end sequencing; RNA-Seq of infected Tomato roots by the vascular wilt fungus Fusarium oxysporum
library_construction_protocol: Samples were collected at the desired timepoints from infected root tissues of tomato plants or in culture grown fungal mycelium. The samples were flash frozen in Liq N2. Infection protocol is as described in the linked Manuscript.  RNA was isolated using the Qiagen Total RNA extraction kit.  TruSeq RNA Library Preparation Kit (Illumina, USA) was used to build the libraries according to the manufacturerâ€™s protocol.