title: NextSeq 500 paired end sequencing; RNA-seq analysis of TCEAL1 knock down with/without docetaxel treatment to enhance docetaxel efficacy in prostate cancer
library_construction_protocol: Samples were prepared from four independent experiments where PC3M cells were treated with control or TCEAL1 siRNA and -/+ docetaxel for 48 hours before collecting total mRNA samples. Total mRNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA was quantified using the NanoDrop 2000 spectrophotometer (Thermo Scientific). For RNA sequencing samples, RNA quality was assessed on a 2100 Bioanalyser (Agilent). Libraries were prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion, Life Technologies) with Poly(A) selection according to the manufacturer's instructions.