title: GSM5005966: HO_asy_H3_4h_II; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Cells were mechanically lysed and chromatin was sheared by sonication to  fragments of 200-500 bp. 40% of the chromatin extract was used for  immunoprecipitation, the purified complexes were treated with proteinase K and crosslinks reversed. DNA was purified by phenol-chloroform extraction and ethanol  precipitation. Strand-specific ChIP-seq libraries were prepared starting from 1-3 ng of DNA using Accel-NGS® 1S Plus Library Kit following manufacturer instructions and 10-12 cycles were used for library amplification. Clean-up steps were performed with SPRIselect beads.