title: GSM5005801: M11_dC34 H3K27ac_ChIP-Seq; Mus musculus; ChIP-Seq
library_construction_protocol: Nuclei were digested with 100 units of MNase at 37°C for 15 min. Mono or di-nucleosomes sized chromatin was incubated with antibodies for 3 h at 4°C and recovered with protein A agarose beads. ChIP DNA (10 ng for H3K27ac and 1 ng for H3K27me3) was processed using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) with manufacturer's instructions. The ends of ChIP DNA were repaired by NEBNext Ultra II End Prep enzyme mix and ligated with NEBNext adaptors. Adaptor-ligated DNA was selected at 175-200 bp size using NEBNext sample purification bead and amplified using the adaptor primers (7 cycles for H3K27ac and 10-11 cycles for H3K27me3). The fragments are purified using NEBNext sample purification beads.