title: GSM5098767: CTL_Beta_5; Danio rerio; RNA-Seq
library_construction_protocol: The main islets from 2-4 pancreata of Tg(sst1.1:eGFP); Tg(ins:NTR*-P2A-mCherry) adult fish (6–10 months old, males and females) were dissected under epifluorescence to eliminate a maximum of non-fluorescent surrounding exocrine tissue and collected and washed in HBSS without Ca2+/Mg2+. Live cell dissociation was performed in Tryple Select 1x solution (GIBCO) supplemented with 100 U/mL collagenase IV (Life Technologies 17104-019) and 40 µg/mL proteinase K (Invitrogen, 25530031) for 10 min at 28 °C, and stopped with 15% FBS. The GFP+ cells, mCherry+ cell and double GFP+ mCherry+ cells were sorted on FACS Aria III under purity mode and after exclusion of the doublets. The purity of the sorted cells was confirmed by epifluorescence microscopy (~95 %). Cells (about 1000-5000/fish depending on the cell type) were immediately lysed with 0.5% Triton X-100 containing 2U/µl RNAse inhibitor and stored at –80 °C. cDNAs were prepared from lysed cells according to SMART-Seq2.0 (Picelli S et al 2014 ) for low input RNA sequencing and libraries were prepared with Nextera® DNA Library kit (Illumina)