title: GSM5005641: TOX4 KO Pol II CUT&Tag rep2; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag experiments were performed as previously described with minor modifications(Kaya-Okur, H.S. et al. ). Briefly, 100,000 cells were used for each experiment. Cells were bound to Concanavalin A-coated beads without fixation and chromatin opening. After primary and secondary antibodies binding, pA-Tn5 transposome binding, and tagmentation, DNA was extracted by PCI. Library was construct by PCR, NEB Phusion(M0530L) was used as protocol.