title: GSM6033925: Day 6 differentiated cells; Homo sapiens; RNA-Seq
library_construction_protocol: Differentiated cells were dissociated using Accutase (Innovative Cell Technologies) for 15-20 minutes on day 6. Cell pellets were resuspended in PBS containing 0.04% bovine serum albumin and filtered through a 40 μm cell strainer (Falcon, Cat#352340) to remove cell clumps before counting with a hemocytometer. Barcoded 3' single-cell libraries were prepared from single cell suspensions using the Chromium Single Cell 3' Library and Gel Bead kit (10X Genomics, Pleasanton, California) for sequencing according to the recommendations of the manufacturer. The cDNA and library fragment size distribution were verified via micro-capillary gel electrophoresis on a Bioanalyzer 2100 (Agilent, Santa Clara, CA). The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing. Eight libraries were sequenced per lane on a NovaSeq 6000 sequencer (Illumina, San Diego, CA) with paired-end 150 bp reads. scRNA-seq on a NovaSeq 6000 sequencer (Illumina, San Diego, CA)