title: GSM5823310: CD45Plus, Pooled_cells_chromium, GEX; Mus musculus; OTHER
library_construction_protocol: Cells from each group were barcoded with different Biolegend anti-mouse Hastags: GGTTGCCAGATGTCA:  HTO6 for group 12hDT TGTCTTTCCTGCCAG:  HTO7 for group 24hDT CTCCTCTGCAATTAC:  HTO8 for group 48hDT After hashtag staining cells were pooled and approximately 18 000 cells suspended in PBS with 0.04% BSA were loaded into the Chromium Controller, in which they were partitioned, their polyA RNAs captured and barcoded using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics). The cDNA were amplified and libraries compatible with Illumina sequencers were generated using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics). The libraries were sequenced on an Illumina NovaSeq sequencer on an SP100 cell flow (read1: 28 cycles; index: 8 cycles; read2: 80 cycles) at a depth of 50 000 reads per cell. scRNA-Seq