title: RNA-Seq of RNA from Brook Trout Liver
design: Brook trout were collected from Pennsylvania headwater stream ecosystems experiencing Unconventional Natural Gas Development or control sites and 50 mg of liver tissue collected and stabilized. RNA was extracted from RNAlater preserved Brook trout Livers using a Qiagen RNeasy Mini kit, and libraries were created with the "SMART Protocol for RNA-Seq". The resulting libraries were then sequenced on an Illumina Hiseq 2000.