title: fecal sample co-extracted with 0.0125 doses of mock community
design: DNA extraction was carried out with the  QIAamp Fast DNA Stool Mini from 1.25 mg of bovine fecal sample, pool BP13. Fecal sample was co-extracted with 0.0125 doses of the mock community: ZymoBIOMICSSpike-inControlI(High Microbial Load) . Amplicons were generated by using a high fidelity PCR system. Amplification was carried our with the two primers: 515F-mod_ILL (Caporaso et al., 2012) and 806R-mod_ILL (Apprill et al., 2016). Quality and quantification of pooled amplicon libraries as well as high throughput sequencing by Illumina technology were performed at the Sequencing Platform,Fondazione Edmund Mach (FEM, San Michele allAdige, Italy). All samples were sequenced in one Illumina MiSeq Standard Flow Cell targeting a minimum depth of 30,000 reads per sample.