title: RNA-Seq of fatbody of exfertile T. rugatulus worker replicate 2 from colony 3
design: Workers were taken from the nest centre, close to the brood pile, which were thus likely to be among the most fertile workers in the nest.  For a subset of of 18 workers in total from six different colonies (four workers from the originally QR fragment (no QR workers from two of the six colonies), eight from QL-1 (two from the six colonies with two QL-1 workers),  six from QL-2), the fat body was dissected on ice  and individually homogenized and stored in 50 L of TRIZOL prior RNA  extraction .  using 50 L of chlorophorm and the RNeasy mini kit (Qiagen). Library preparation was conducted according to standard protocols at BGI Hongkong, sequencing 100bp paired reads on an Illumina HiSeq 4000.