title: 0.4 ng/L nano-Pd exposed Zebrafish
design: Palladium nanoparticles (Pd NPs) have been the reliable substitutes as conventional catalysts for its high efficiency and conversion of CO, hydrocarbons, and NO into less harmful CO2, water and nitrogen. Due to surface abrasion of converters, Pd as ultrafine/nanoparticles are liberated into the environment causing possible health effects. Also, Pd leaches to the aquatic system through heavy precipitation and runoff. Here, we aimed to profile the transcriptome of Danio rerio exposed to nano-palladium at 0.4 ng/L (environmental concentration). 500 ng of total RNA of 0.4 ng/L nano-Pd fish was isolated and the enriched mRNAs were reverse transcribed to form cDNA. The concentrations above 2 ng/L were used for the transcriptome analysis with the reference genome. Around 64792 genes were expressed in 0.4 ng/L. There were 3153 DEGs in Control vs 0.4 ng/L with 2900 up-regulated and 253 down-regulated genes. Also, for 22 ng/L vs 0.4 ng/L, 3347 genes were significantly expressed with 2101 up-regulated and 1246 down-regulated genes. The transcriptome screening is evident that nano-Pd can cause possible effects in organisms even at low concentrations.