title: PacBio HiFi sequencing of T3F02_SC_FF
design: PacBio HiFi SMRTbell Libraries (https://www.pacb.com/) were prepped following the SMRTbell Express Template Prep Kit 2.0 procedure. The UC Davis Sequencing Center used a Megaruptor to shear DNA to an average fragment length of ~15kb. The input for the library prep was 5.6 ug of DNA and the library was size selected down to be 11kb. Prepped DNA was run on a Sequel II machine and sequencing data was collected as recorded movies of nucleotides. Each movie collection lasted for 30 hours per run. Additional movies were collected until the amount of sequencing data for each sex was ~25-30x coverage.