title: RNAseq of Sus scrofa: single blastocyst total RNA sequencing
design: Total RNA was extracted from a single Sus scrofa blastocyst by PicoPure RNA Isolation Kit (Arcturus). RNA-seq library was constructed from the blastocyst total RNAs by Trio RNA-Seq Library Preparation Kit (Tecan), according to the manufacturer's instructions.  Each NGS library was sequenced by Illumina NovaSeq instrument using 100 bp paired-end sequencing chemistry.