title: NGS P. putida RB-TnSeq library: LB T=0 Replicate C
design: 1 mL cell pellet used to extract gDNA by the GeneJet Genomic DNA Purification Kit (Thermo Scientific, USA). Specific primers used to amplify the Tc1 associated barcode region with Q5 High-Fidelity 2X Master Mix (New England Biolabs, USA). Samples pooled and sent to Genewiz for sequencing