title: SSU rDNA of human feces metagenome: female KT 1
design: Microbial DNA was extracted from approximately 0.2 g fresh fecal sample of each object using a DNeasyPowerSoil Kit (QIAGEN, Germany). The V4-V5 hypervariable region of 16S rRNA gene was amplified using the universal primers 515F and 907R as previously described.