title: Illumina WGS of Manihot esculenta: 60444 (Transgenic empty vector line, 4220) leaf
design: Illumina sequencing: Leaf material or FEC material was collected cassava genotype for whole genome Illumina sequencing. DNA was extracted using the DNeasy Plant Mini Kit (QIAGEN, Germany). DNA samples were sent to the Functional Genomics Center Zurich (FGCZ) for Illumina sequencing. DNA libraries were prepared using the Illumina TruSeq Nano DNA High Throughput Library Prep Kit (20015965), following the manufacturer's protocol (Illumina, San Diego, California). Libraries were sequenced using an Illumina NovaSeq system for 2  151 cycles, according to the manufacturer's instructions (Illumina, San Diego, California). On average 100X Illumina paired-end (PE) data were collected per sample.