title: DNA-Seq of strain USGS_26222_1_NV
design: Biomass was collected from cultures grown for 24 hours in liquid LGMM + YE media, flash frozen in liquid nitrogen, and lyophilized. Lyophilized biomass was homogenized using glass beads, treated with RNAse, and extracted using  Phenol:Chloroform:Isoamyl alcohol. Illumina sequencing libraries were prepared using the NEBNext Ultra II Library Prep Kit paired-end library construction and barcoding for multiplexing.