title: RNA-seq of larvae
design: Fifth instar larvae with consistent growth were selected and used for infection with Beauveria bassiana. After 24 hours, the samples of treated and control groups were immediately frozen in liquid nitrogen. Total RNA was extracted.  The cDNA library of each sample was constructed using NEBNextH mRNA Library Prep Reagent Set  following the manufacturers protocol. The RNA strand was then degraded by RNaseH, and the second strand was synthesized by the DNA polymerase I using dNTPs as raw material. After purification, double-stranded cDNA was repaired at the end, followed by the addition of A tail and the connection of sequencing adapters. AMPure XP Beads were used to screen about 250-300bp of cDNA. AMPure XP Beads were again used to purify each cDNA and used as templates for PCR amplification to create the cDNA library.