title: DNA-seq T7 RNAP LacZ amplicon: Platinum II Taq
design: Library of T7 RNAP mutants: LacZ amplicon. Libraries were sequenced on the Illumina MiSeq instrument using the MiSeq Reagent Kit v2 (300-cycle) at 2  150 bp PE mode. For mutation analysis, amplicons were sequenced with a minimum coverage of 73,769.