title: 16S_rRNA_gene_Bacteria:_soil_upper_horizon
design: The variable regions V3-V4 of 16S rRNA genes were amplified out using the Illumina protocol and 16S metagenomic sequencing library preparation, using primers 341F and 805R. After amplification, the libraries were purified and equimolarly mixed with a SequalPrep normalization plate kit. Libraries were sequenced in two separate runs by Illumina MiSeq with 2  250 bp paired reads using a MiSeq v2 reagent kit (500 cycles).