title: Transcriptome of Outdoor UV mutant 2 of C. vulgaris
design: The final PCR product (sequencing library) was purified with HighPrep beads, followed by library quality control check. The Illumina-compatible sequencing library was initially quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distribution was analyzed on Agilent TapeStation. The libraries were pooled in equimolar amounts to create a final multiplexed library pool for sequencing on an Illumina sequencer for 75bp paired-end chemistry. Sequencing was carried out in Illumina NextSeq500 using Paired-end (150*2) chemistry.