title: 22 ng nano-Pd treated zebrafish
design: Palladium nanoparticles (Pd NPs) have been the reliable substitutes as conventional catalysts for its high efficiency and conversion of CO, hydrocarbons, and NO into less harmful CO2, water and nitrogen. Due to surface abrasion of converters, Pd as ultrafine/nanoparticles are liberated into the environment causing possible health effects. Also, Pd leaches to the aquatic system through heavy precipitation and runoff. Here, we aimed to profile the transcriptome of Danio rerio exposed to nano-palladium at 22 ng/L (environmental concentration). 500 ng of total RNA of 22 ng/L nano-Pd fishes was isolated and the enriched mRNAs were reverse transcribed to form cDNA. The concentrations above 2 ng/L were used for the transcriptome analysis with the reference genome. Around 66612 genes were expressed in 22 ng/L with respect to the total reads. The comparison of Control vs Treatment I observed of 2738 DEGs, with 819 up-regulated and 1919 down-regulated genes. The up-regulation and down-regulation of the genes were evident that the nano-Pd affects the physiological and endocrine functions of zebrafish at higher concentrations.