title: small RNA-Seq of homo sapien: plasma female
design: MicroRNA was isolated from 200 L plasma samples using the QIAGEN miRNA serum/plasma kit (Qiagen, Hilden, Germany) according to the manufacturers instructions and stored at -80C. Library preparation and sequencing was performed by Qiagen (Valencia, CA) using the QIAseq miRNA Library Kit and QIAseq miRNA 48 Index IL kits as per manufacturers instructions. Amplified cDNA libraries underwent single-end sequencing by synthesis (Illumina v1.9).