title: RNA-seq of colonic crypts:  tamoxifen induction of Kras and corn oil diet
design: Library was produced using the TruSeq RNA Library Preparation Kit v2 from Illumina following manufacterer's instructions.  Libraries were made starting with 250 ng of RNA..  Libraries were quantitated using the DNA High Sensitivity trace from the Bioanalyzer and quantitation from the NEBNext Library Quant kit fro Illumina from NEB.  The libraries were pooled equimolarly and sequenced on the HiSeq 2500.