title: Case04_NTUH_1_055 sepsis blood mNGS
design: The cfDNA was extracted from 200L plasma utilizing TIANamp Micro DNA Kit (DP316, TIANGEN BIOTECH). Subsequently, the DNA libraries were constructed through consecutive reactions that include end-repair, adaptor ligation, PCR amplification and purification. The qualified libraries were further subjected to DNA circularization using MGIEasy circularization kit followed by DNA nanoball (DNB) formation using rolling circle replication mechanism. Finally, upon DNB formation the sequencing was performed on a MGISEQ-200 sequencer (MGI Tech Co. Ltd.), resulting in greater than equal to 300 million 100-base single end reads.