title: MiSeq raw reads of Bacillus paralicheniformis: from the roots of durum wheat Triticum turgidum
design: Total genomic DNA isolation using the Wizard Genomic DNA Purification Kit (Promega Madison, WI, USA) following the manufacturers instructions. The isolated gDNA quality was assessed by gel electrophoresis in a 1% agarose gel stained with Red Safe (Intron, Bio-tek, Korea) visualized by using Gel Doc XR Gel Documentation System (Bio-Rad, Hercules, CA, USA). The concentration and quality of the gDNA were determined by using a spectrophotometer (Smart-Spec plus spectrophotometer, BIO-RAD, Hercules, CA, USA). The gDNA was sent to the service provider Macrogen Inc. (Seoul-Korea) to be sequenced using Illumina NovaSeq platform, library type: TruSeq Nano DNA (350), type of reads: paired-ends, read length: 151 bp. To assess the quality of the raw reads, FastQC v0.11.9 was used. Fastp was used to trim the adaptor sequences and remove bad reads (too low quality, too short, or too many N). The sequence reads were de novo assembled into 24 contigs