title: RNA-seq of Drosophila melanogaster: Female midgut from mated flies
design: Female guts were collected two days after mating to SP0 or SPWT males, and from virgin females. RNA was isolated from 60 guts per sample per replicate, using a modified TRizol extraction protocol (Life Technologies). Following RNA extraction, 3end RNA-seq libraries were prepared using QuantSeq 3 mRNA-Seq library prep kit, according to manufacturers instructions (Lexogen).