title: gene marker-Seq of thermal spring Stepanka microbial community_autumn
design: 20 L of thermal water was filtered using the 0.22 um PES-membrane filters; DNA from the filtered cells was isolated using a DNeasy PowerWater Kit; the genomic DNA was subjected in duplicates to PCR amplification of 16S rRNA genes using primers and conditions reported before (10.1016/j.chemosphere.2019.04.036); PCR products were purified using AMPure XP Beads and submitted to final library preparation and sequencing in the DNA Core Lab of the University of Alaska Fairbanks, USA; prior to sequencing, the concentration of amplicons was normalized to 1-2 ng. L-1 using a SequalPrep Kit, the samples were pooled, subjected to 8-cycle PCR to add specific adapters and sequenced on the Illumina MiSeq