title: ONT PromethION sequencing of Canis lupus familiaris: Nala (German Shepherd)
design: Genomic DNA (1 g) was prepared for ONT sequencing using the 1D genomic DNA by ligation kit (SQK-LSK109, ONT) according to the standard protocol. Long fragment buffer was used for the final elution to exclude fragments shorter than 1,000 bp. In total, 119 ng of adapted DNA was loaded onto a FLO-PRO002 PromethION flow cell and run on an ONT PromethION sequencing device using MinKNOW (18.08.2) with MinKNOW core (v1. 14.2). Base-calling was performed after sequencing with the GPU-enabled guppy basecaller (v3.0.3) using the PromethION high-accuracy flip-flop model with config dna_r9.4.1_450bps_hac.cfg.