title: COI Sponge: WB-00590
design: Briefly, the DNA of frozen (-80 C) samples was extracted using the DNeasy Power Soil Kit (Qiagen, Venlo, The Netherlands). After quality controls of the extracted DNA,  fragments of the cytochrome c oxidase subunit I (COI) were amplified  in a PCR (94 C/5 min - (94 C/30 sec - 42 C/1 min - 72 C/30 sec) x 30 cycles - 72 C/7 min) using the primer pair dgLCOF- COXIR. PCR products were verified by gel electrophoresis and sequencing was conducted on an ABI 3730XL DNA Analyser (Applied Biosystems, USA) by Eurofins Genomics.