title: Whole genome sequencing of heat exposed Arabidopsis thaliana plants
design: gDNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies). Libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) as per the manufacturers recommendations. Adapters and PCR primers were purchased from IDT. Size selection of libraries contained the desired insert size has been performed using SparQ beads (Qiagen). Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument.