title: RNAseq of AgN23 grown in glucose
design: Three replicates were prepared for the libraries construction. RNA-seq libraries have been prepared according to Illuminas protocols using the Illumina TruSeq Stranded mRNA sample prep kit to analyze mRNA. Briefly, ribodepletion was carried out to clean up mRNAs using poly-T beads. Then, RNAs were fragmented to generate double stranded cDNA and adaptators were ligated to be sequenced. Eleven cycles of PCR were applied to amplify libraries. Library quality was assessed using a Fragment Analyzer and libraries were quantified by QPCR using the Kapa Library Quantification Kit. RNA-seq experiments have been performed on an Illumina HiSeq3000 using a paired-end read length of 2x150 bp with the Illumina HiSeq3000 sequencing kits.