title: 16S seq of temperate forest mineral soil
design: Genomic DNA extraction was performed on 0.25 g of rhizospheric soil or 0.1 g of grinded roots using the MoBio PowerSoil DNA isolation kit following the manufacturer's instructions. Metabarcoding library preparation and sequencing were performed as described in Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using the Qiagen HotStar Taq DNA polymerase and primers ITS9F (5-GAACGCAGCRAAIIGYGA-3) and ITS4R (5- TCCTCCGCTTATTGATATGC-3) targeting the ITS2 region of fungi for the first PCR (amplicon PCR). Sequencing was performed on an Illumina MiSeq platform with a MiSeq Reagent Kit v2 (500 cycles) at the Illumina Sequencing Platform, Nucleic Acids Solutions, Aquatic and Crop Resource Development, National Research Council Canada-Saskatoon.