title: 3'Seq of Mus musculus: B cell lymphoma
design: RNA was isolated from cryo-preserved tumor tissue using a commercial kit (Qiagen, Cat. No. 74104). 3mRNA libraries were generated from total RNA using the Lexogen QuantSeq kit according to the standard protocol. After validation (2200 TapeStation; Agilent Technologies) and quantification (Qubit System; Invitrogen, Waltham, USA) pools of cDNA libraries were generated. The pools were quantified using the KAPA Library Quantification kit (Peqlab, Erlangen, Germany) and the 7900HT Sequence Detection System (Applied Biosystems, Waltham, USA)