title: pacbio_long
design: Total RNA from different samples was mixed at equal ratios. Poly(A) RNA was isolated from total RNA using Dynabeads Oligo (dT)25 (Thermo Fisher). The first cDNA strand was synthesized from purified polyA RNAs using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA). After PCR optimization, largescale PCR was performed to synthesize the second cDNA strand without size selection. Equimolar mixed libraries of > 1 kb fragments were prepared with the SMRTbell Template Prep Kit 1.0. Sequencing was performed on a PacBio Sequel II platform.