title: 4C-Seq library amplified from chr14_rs10873246 viewpoint
design: 10 million HDF cells were fixed in 2% formaldehyde, and then the cells were lysed to isolate nuclei. The chromatin was digested in situ with the four-base cutter MboI (New England Biolabs, Ipswich, MA) for 16 hours at 37C followed by in situ ligation with T4 ligase (Thermo Fisher Scientific, Waltham, MA). Chromatin was decrosslinked at 65C overnight, followed by a second digestion with the four-base cutter NlaIII (New England Biolabs, Ipswich, MA). Digested fragments were diluted and self-ligated using T4 ligase to generate circular chromatin conformation capture (4C) library. The efficiency of each digestion and ligation step was validated on agarose gels. We then Divergent primers with Illumina sequencing adapters were used to amplify DNA fragments concatenated with our viewpoints of interest and generate 4C libraries.Viewpoint: chr14_rs10873246, Chromosome: chr14, Position (hg38): 71,686,976, Forward Primer: TGGAAACTGTAGGACTGAGA, Reverse Primer: TGAGGCATAAAGAGAAGGTAC