title: Tumor Mutation load panel for chordoma patient
design: DNA was extracted from one formalin-fixed, paraffin-embedded chordoma tumor tissue by using GeneRead DNA FFPE kit (Qiagen, Germany). DNA sequencing (NGS) was performed by using Ion AmpliSeq targeted amplification technology with custom Tumor Mutation Load (TML) panel to calculate Tumor Mutation Burden (TMB) and for checking CCP panel. The bioinformatic workflow for sequencing data analysis was based on Torrent Suite software (version 5.10.1).