title: IV01_KUELA227
design: gDNA was subjected to optical density measurements in NanoDrop and Qubit (ThermoFisher Scientific, Waltham, MA USA), DNA migration in agarose gel electrophoresis or and Agilent Tapestation to confirm the purity and concentration prior to library preparation. Genomic DNA was fragmented and ligated to unique adapters using Swift 2S Turbo DNA library prep kit (Swift Bioscience, Ann Harbor, MI, USA). The resulting library was tested for size distribution and concentration using Qubit and tapestation. The libraries were then sequenced on NextSeq 500 (Illumina, San Diego, CA) according to the standard operation. Paired-end 150 nucleotide (nt) reads were generated.