title: Tag based RNA-Seq of Sciaenops ocellatus: whole body larva
design: 3' RNA based library prep using single tag from polyA tail to sequence random narrow window near 3' end of each transcript. cDNA amplified using PCR, labeled with barcode, and used gel-extraction to purify size fraction (150-200). See Meyer, Aglyamova, & Matz 2011.