title: cDNA-Seq of mus musculus (Stat6ko): medLN CD24- sort 2
design: Total RNA was prepared from frozen cell pellets using RNeasy Micro Kit  from QIAGEN. RNA was quantified using a Quantus Fluorometer (Promega) and RNA integrity was checked using a Fragment Analyser (Agilent). Library preparation and RNA sequencing were contracted out to New Zealand Genomics Limited. Samples were spiked with External RNA Controls Consortium mix (Ambion; Life Technologies). Ribosomal RNA was depleted using RiboZero (Illumina) and the libraries prepared with Illumina TruSeq kits.