title: rnaseq_musculus_female_brain
design: All RNA samples were tested for purity using a Bioanalyzer 2000 and had RIN values of >9.3.  cDNA libraries were made by the OHSU Massively Parallel Sequencing Shared Resource using the TruSeq stranded total RNA with ribo-zero kit (Illumina). Library quality was assessed using a TapeStation 220 and followed by quantification using quantitative PCR. Libraries were sequenced using paired end sequencing on a NovaSeq 6000 using a 200 cycle S4 flow-cell kit containing 10 million clusters.