title: RNAseq of mus musculus Puma -/- Female mock infection
design: RNA was purified using the RNeasy kit (Qiagen) according to the vendors instructions.  Ten micrograms total RNA served as starting material with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), and cDNA libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).  Samples of cDNA from all mice were normalized for concentration, pooled, and sequenced on their own lane of a NOVASeq 6000 sequencer at 100 base-pair paired-end reads and yielded at least 10 million reads.