title: WGS of anaerobic fermenter sludge metagenome: bioreactor
design: Biomass samples for shotgun metagenomics analysis were collected from internal recycle lines of the bioreactors. Samples were centrifuged, supernatant was discarded, and biomass was stored at -80C. Genomic DNA was extracted using PowerSoil DNA Isolation kit (MO BIO Laboratories Inc., Carlsbad, CA).