title: RNA-seq of Homo Sapiens:AC16 cardiomyocyte
design: sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturers instructions. RNA  sequencing  was  performed  on  the  Illumina  HiSequsing  a 2x150bp   paired-end   configuration.   Raw   sequence   data   (fastq   files)   were   trimmed   using Trimmomatic v.0.36. The trimmed reads were mapped to the Homo sapiensGRCm38 (available on ENSEMBL) using the STAR aligner v.2.5.2b. Unique gene hit counts were calculated using the Subread package v.1.5.2. and the unique reads that fell within exon regions were counted