title: PacBio Sequel sequencing of Canis lupus familiaris: Nala (German Shepherd)
design: Genomic DNA was prepared from 12 mL of fresh blood using the genomic-tip 100/G kit (Qiagen, Hilden, Germany) with supplemental RNase and proteinase K treatment. Isolated genomic DNA was further purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) and size selected on Sage BluePippin gels (Sage Science, Beverly, MA, USA). The library was sequenced on a PacBio Sequel with 2.0 chemistry recording 10 h movies at the Ramaciotti Center for Comparative Genomics at University of New South Wales, across 15 SMRT cells.