title: GSM4782065: SLE1_HiC_Lib2; Homo sapiens; Hi-C
library_construction_protocol: About one million monocytes were crosslinked by 40 ml 1% formaldehyde solution at room temperature for 10 min and 2.5 M Glycine was added to quench the crosslinking reaction. Supernatant was removed and the cells were washed three times by 1 X PBS. Crosslinked cells were resuspended in 500 µL of ice-cold Hi-C lysis buffer and rotated at 4 °C for 30 min. in situ MboI Hi-C were assembled based on Illumina-compatible protocols.