title: GSM5419669: Mesothelial cells-HG+Melatonin(MT)_6H; Homo sapiens; RNA-Seq
library_construction_protocol: Mesothelial cells were treated with normal or 236mM glucose in the presence or absence of 200mM melatonin and cells were collected after 6 and 12 hours. For melatonin intervention, the indicated concentration of melatonin was added to cells 12 hours prior to exposure to high glucose.RNA was extracted from the cells and mRNAs were enriched via poly A. Illumina NEBNext® Ultra™ RNA Library Prep Kit (Cat#E7530L) was used to construct sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols