title: ONT PromethION WGS of Cooinda the Alpine Dingo: blood
design: DNA was prepared for ONT sequencing using the 1D genomic DNA by ligation kit (SQK-LSK109, ONT) according to the standard protocol. Long fragment buffer was used for the final elution to exclude fragments shorter than 1,000 bp. Adapted DNA was loaded onto a FLO-PRO002 PromethION flow cell and run on an ONT PromethION sequencing device using MinKNOW (18.08.2) with MinKNOW core (v1. 14.2). Base-calling was performed after sequencing with the GPU-enabled guppy basecaller (v3.0.3) using the PromethION high-accuracy flip-flop model with config dna_r9.4.1_450bps_hac.cfg.