title: GSM5466398: 32771R_DMSO_S15_R1_001.fastq; Homo sapiens; RNA-Seq
library_construction_protocol: Enzalutamide or vehicle treated patient derived explants preserved in RNAlater were homogenized with the Precellys24 Tissue Homogenizer (Bertin Technologies, France) using 0.5 mL Precellys24 tubes containing 1.4 mm ceramic beads and 500 mL Tri Reagent (Sigma). RNA was then extracted using a Qiagen RNeasy mini kit adapted for Tri Reagent using the manufacturer instructions. Libraries were generated using Illumina TruSeq RNA kits; Illumina NextSeq 500 (High Output v2) single-end 100 bp reads.