title: GSM5397586: BMC_10_WT naïve (s26); Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted into RLT buffer or  lysed in RLT following 3hr in vitro stimulation as described above Libraries were generated by Emory Genomics Core (EIGC) as follows: Cells were sorted directly into RLT + 1% BME buffer and RNA isolated using the Quick-RNA MicroPrep kit (Zymo,11-328M). 2000 cell equivalent of RNA was used as input to generate cDNA using the SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara, 634888). cDNA was quantitated by Qubit (ThermoFisher Scientific) and 200 pg of cDNA was tagmented and PCR amplified using the NexteraXT kit (Illumina, FC-121-10300) to generate final sequencing libraries. Libraries were quantitated by Qubit, pooled at equimolar ratios, and sequenced using 75 bp paired-end Illumina chemistry. RNA-seq performed as follows: Libraries were sequenced in an individual lane of an S1 flowcell on the NovaSeq6000 with a PE100 configuration using a NovaSeq 6000 SP Reagent Kit.