title: GSM5467438: HCT-116 cells with DDX3X degron, lentiCTRL, auxin treated, ribosome profiling, replicate 1; Homo sapiens; OTHER
library_construction_protocol: Cells were treated with 100ug/ml cycloheximide, flash frozen, lysed and treated with 2u/ul RNaseI (Ambion). Illustra MicroSpin Columns S-400 HR (GE Healthcare) were used to enrich for ribosome complexes and ribosome protected RNA fragments were extracted using the DIRECTZol RNA prep kit (Zymo) Library construction for RPFs was performed as described in McGlincy et al. PMID 28579406