title: GSM5106050: Illumina_WT-0-replicate 1_short_read_reanalyzed; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested for RNA isolation using RNeasy kit with on-column DNase digestion (Qiagen). Iso-Seq SMRTbell libraries containing circular templates were generated using NEBNext Single Cell RT Enzyme Mix and recommendations by the manufacturers recommendations for long-read transcripts using the Iso-Seq™ Express Template (PN: 101-901-700) and indicated quality control steps.  Sequencing was performed using the Pac Bio Sequel II system with SMRT Cell 8M.