title: GSM5105511: 10x Single Cell RNAseq of KO Day1 Post IM 2019; Mus musculus; RNA-Seq
library_construction_protocol: 10x v3 3' kit was used according to manufacturer's instruction. Single cell RNA sequencing libraries were prepared using 10x genomics platform according to manufacturer's instructions (Single cell 3' solution). Sequencing libraries were loaded at 1.4 pM on an Illumina NextSeq500 with NextSeq 500/550 Mid Output v2 kit (150 cycles) (Illumina). Cell Ranger software (v3.0.2) (10x Genomics) was used to demultiplex Illumina BCL files to FASTQ files (cellranger mkfastq) to perform alignment (to mouse GRCm38/mm10 genome), filtering, UMI counting and to produce gene-barcode matrices.