title: GSM5792277: SNU449 PLKO A-1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA of the cells was extracted by TRIzol method A total amount of 2 µg RNA per sample was used as input material for the RNA sample  preparations. Sequencing libraries were generated using VAHTS mRNA-seq v2 Library Prep  Kit for Illumina following manufacturer's recommendations and index codes were added to  attribute sequences to each sample. Briefly, mRNA was purified from total RNA using  poly-T oligo-attached magnetic beads. Fragmentation was carried out using fragmentation  buffer. First strand cDNA was synthesized and second strand cDNA synthesis was  subsequently performed. Remaining overhangs were converted into blunt ends. After  adenylation of 3' ends of DNA fragments, adaptor with hairpin loop structure were ligated. Then the PCR was performed. At last, Qubit HS quantification, Agilent 2100  Bioanalyzer/Fragment Analyzer 5300 quality control, the final library size of