title: GSM5107419: RNA-Seq splenic NK - MUT 7; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspension of splenocytes was enriched for NK using DX5 Microbeads (Miltenyi) before sort purification for NK cells (CD49b+,CD3e-). Each mouse served as a biological replicate. 3-5 x 10^5 NK cells from each mouse were used for isolation of total RNA using the RNeasy Plus Micro Kit (Qiagen) Libraries were generated with the SMART-seq HT Kit (Takara Bio no. 634438)