title: GSM6282724: ZnSO4 dose level 2 bio rep 2 [Zn_P1_B3_S9]; Pimephales promelas; RNA-Seq
library_construction_protocol: Larvae remained in their 1 mL deep wells, exposure solution was removed and replaced with lysis buffer, a bead was added to each well, and subjects were homogenized in their wells. Libraries were prepared from 250 ng RNA using the SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Greenland, NH) according to the supplied protocol.