title: GSM5899128: RNA-Seq H1299-EVG418-4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from 200 µl of lysate using 750 µl of TRIzol LS and purified using the Zymo RNA Clean and Concentrator-5 kit with modifications to the manufacturer's instructions: Recovered RNA was treated with DNAse I at 37°C for 10 min prior to addition of RNA Binding Buffer, and isolated RNA was eluted in 15 µl nuclease-free water. Prior to library preparation, purified RNA was quantified and checked for RNA integrity with Agilent Bioanalyzer 2100 using the RNA 6000 Nano kit according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols