title: GSM5006968: Endothelium (Endo) E8.5 [E8.5 Endo_37]; Mus musculus; RNA-Seq
library_construction_protocol: yolk sac was dissociated with trypsin (E7.25-7.775) or collagenase/dispase (E8.25-E10.5). Cells were sorted by population first then captured on Fluidigm C1 integrated fluidic circuit (IFC) with the capacity for 96 individual cells and a 10-17um capture aperture Libraries were constructed using the Nextera XT kit and Illumina 96 index kits according  to the Fluidigm protocol modified from the Illumina protocol to use ¼ of the kit per 96  well plate (Illumina Protocol #15031942 Rev C “Nextera XT DNA Sample Preparation  Guide”; Fluidigm Protocol #PN100-7168 G1 “Using the C1 Single Cell Auto Prep  System to Generate mRNA from Single Cells and Libraries for Sequencing”)