title: GSM5419847: WGBS_SCNT_4c_TDG_rep2; Sus scrofa; Bisulfite-Seq
library_construction_protocol: The DNA extraction of PFFs or 4-cell SCNT embryos (50 cells) are performed Bisulfite-seq by using PBAT protocol as previously described (Miura et al., 2012; Smallwood et al., 2014). Bisulfite converted DNA is used for library construction using the index primer and the universal primer (NEB), and 2× KAPA HiFi HotStart ReadyMix.