title: GSM5914796: intron19 of ARFGEF1 sequencing; Homo sapiens; OTHER
library_construction_protocol: To examine the full-length sequence of intron19 of ARFGEF1, we conducted the long-range PCR experiment by PrimeSTAR GXL DNA polymerase (TaKaRa, Cat. R050B). A total of 100 fmol of gel-purified PCR products was sequenced by the protocol of ONT Amplicons by Ligation (Oxford Nanopore Technologies, SQK-LSK109). Briefly, DNA was repaired by FFPE (New England Biolabs, Cat. M6630) and end-prepped/dA-tailed by using the NEBNext End Repair/dA-tailing module (New England Biolabs, Cat. E7546). Next, the kit-supplied sequencing adapters are ligated onto the prepared ends. After purification by AMPure XP beads, a total of 50 fmole DNA was sequenced by an R9.4 Flow cell.