title: Consistent patterns of microbial diversity, prediction accuracy and associated bacterial organisms between pediatric ulcerative colitis and healthy children using 70S rRNA and metagenomic shotgun sequencing data
design: Metagenomic libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina) and Illumina Nextera XT Index v2 Kit A and B following the manufacturers protocols. Library qualities were assessed on Agilent High Sensitivity DNA Bioanalyzer chips. Libraries were pooled and sequenced on an Illumina NextSeq500 High Output v2 flowcell on an Illumina NextSeq 500 System, producing 2_x0002_150bp paired-end reads. All samples and control reads were pre-processed, and quality filtered using Trim Galore. Host-derived reads were removed using KneadData.