title: PacBio WGS of Streptococcus pneumoniae TIGR4
design: PacBio sequencing was performed by the Lausanne Genomic Technologies Facility (GTF).  High-molecular weight DNA extraction was size-fractionated, then used to create a 15 kb insert library. The resulting DNA was sequenced on a PacBio Sequel II machine, in a pool of bacterial samples on a single SMRT-cell.