title: GSM5421383: 2-CCA14_T; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from CCA primary tissue and CCAOs cultured in EM and branching medium conditions was collected in lysis buffer (QIAzol lysing reagent, Qiagen) and the RNA was isolated using the miRNeasy mini kit (Qiagen) according to the manufacturer's protocol Sequencing data were uploaded to the Galaxy Web platform public server usegalaxy.org (Afgan E., Baker D., van den Beek M., Blankenberg D., Bouvier D., et al., 2016 The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2016 update. Nucleic Acids Res. 44: W3–W10). Sequences were trimmed for adapters using Trim Galore! (version 0.4.3.1), and mapped using RNA STAR (version 2.6.8a) against the human reference genome GRCh38).