title: GSM5107629: Nicotine_Saline_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Offspring lungs were collected at ped3 and RNA was isolated and purified using Macherey-Nagel purification kit (Germany). Three to 5 offspring lung were used for each group, namelly control-saline (C-S), control-elastase (C-E), nicotine-saline (N-S) and nicotine-elastase (N-E) group. cDNA libraries were constructed by the Genomic platform of the University of Geneva using the Illumina TruSeq RNA Sample Preparation Kit (CA, USA) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols