title: GSM5230419: Donor_4_CBFB_RNA; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed with Zymo QuickRNA lysis buffer and isolated with the Zymo QuickRNA micro kit (Zymo, Cat #R1050) using the manufacturer's protocol. RNA samples were treated with 1.5 ul of Turbo DNase (Invitrogen, Cat # AM2238) and then cleaned up using the Zymo RNA-5 Clean and Concentrator (Zymo, Cat #R1016). Isolated RNA integrity and concentration was checked using Agilent RNA Screen Tapes (Agilent, Cat #5067-5576) RNA was submitted to the UC Davis DNA Technologies and Expression Analysis Core to generate 3' Tag-Seq libraries with unique molecular indices (UMIs). Barcoded sequencing libraries were prepared using the QuantSeq FWD kit (Lexogen, Vienna, Austria) for multiplexed sequencing according to the recommendations of the manufacturer (Lexogen). The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a Bioanalyzer 2100 (Agilent, Santa Clara, CA). The libraries were quantified by fluorometry on a Qubit fluorometer (LifeTechnologies, Carlsbad, CA), and pooled in equimolar ratios. Samples were sequenced on a HiSeq 4000 sequencer (Illumina, San Diego, CA).