title: GSM4781967: E12.5 R1_5M; Mus musculus; RNA-Seq
library_construction_protocol: Ventral r1 was dissected in the 5% FBS (fetal bovine serum; Millipore, Cat#ES-009-B) in L-15 (Gibco, Cat#11415-049). The ventral r1 was separated from the brain by using visible landmarks (such as the constriction of midbrain-hindbrain boundary).  The pieces were washed 3 times with cold 500ul 1xPBS (Lonza, Cat#BE17-516Q) and dissociated using the Papain Dissociation System (Worthington, Cat#LK003150) according to the manufacturer's protocols. The dissociated cells were collected on 10x Genomics Chromium Single Cell 3'RNAseq platform. The collection rate was 5000 cells/sample and the cDNA synthesis was done using the Chromium™ Single Cell 3' Reagent version 3 chemistry. The 10XGenomics E12.5 and E13.5  cDNA libraries were sequenced on Illumina NovaSeq 6000 system using read lengths: 28bp (Read 1), 8bp (i7 Index), 0 bp (i5 Index) and 89bp (Read 2). Read quality was assessed by running FASTQC (version 0.10.1)