title: Metagenomics - Microbial profiling of Psychedelic mushroom fruiting body
design: Total metagenomic DNA was extracted from the psychedelic mushroom fruiting body samples following the modification of Silva et al., (2014), Hassan et al., (2018) and Srinivasan et al., (2019) and V3 - V4 regions were amplified by using primers. The first-round PCR products were used as templates for second-round amplicon enrichment of PCR. At the same time, indexed adapters were added to the ends of the 16S rRNA amplicons to generate indexed libraries ready for downstream NGS sequencing on Illumina Miseq.