title: GSM5425438: Cecum contents, CNP4; rat gut metagenome; OTHER
library_construction_protocol: Cecum contents were collected at 4 0C. the microbial genome DNA of rat cecum contents was extracted and purified. Then, the V4 region of 16S rDNA was amplified by PCR. After agarose gel electrophoresis, gel extraction, quantification, and library preparation, sequencing was performed on Illumina HiSeq with MiSeq Reagent Kit. DNA libraries were prepared with Agilent SureSelectXT Methyl-seq Library Pre kit and then hybridized with hybridization reagents, blocking agents and the SureSelect capture library. Illumina HiSeq