title: PacBio HiFi CCS of Euphorbia peplus: leaf/bract tissue
design: Plant tissue was ground in liquid nitrogen with a mortar and pestle and transferred to 2mL microcentrifuge tubes.  A CTAB extraction method using chloroform:isoamyl alcohol 24:1, including treatment with Proteinase K and RNAse A, was used to extract the DNA.  DNA samples were sent to HudsonAlpha Institute for Biotechnology for PacBio HiFi CCS sequencing, where they were sheared with a Diagenode Megaruptor, size selected for 18kb fragments on the SageELF electrophoresis system and sequenced on a PacBio Sequel-II sequencer.