title: GSM5110460: deltaFoxp3.CD4_repB; Mus musculus; RNA-Seq
library_construction_protocol: A single cell suspension was obtained of murine splenocytes after mechanical dissociation and red blood cell lysis. Cells were stained with fluorophore-conjugated +- DNA-conjugated antibodies for cell sorting by flow cytometry and sample pooling for single cell RNAseq library construction (10x Genomics, Single Cell 3′ Reagent Kit (V2 or V3 chemistry) RNA libraries were prepared for sequencing using standard 10X Genomics Single Cell 3′ Reagent Kit (V2 or V3 chemistry)