title: ITS amplicon metagenomics of TX grad plant leaf tissue
design: Plant DNA was extracted ca. 50 mg surface-sterilized leaf tissue MoBio PowerPlant Pro DNA Isolation Kits. Gene amplification of 10 ug uL-1 DNA was performed in triplicate using the ITS1f (5 CTTGGTCATTTAGAGGAAGTAA 3) and ITS2 (3 CGTAGCTACTTCTTGCGTCG 5) fungal primers and pooled for sequencing on MiSeq v2, 2x250 PE at the University of Texas Genome Sequencing and Analysis Facility (Austin, TX).