title: Amplicon sequencing of the bacterial 16S rRNA gene for venous stasis ulcer microbiome
design: TCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3, reverse primer: 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3). The targeted regions were amplified via PCR, purified, attached to Illumina sequencing adapters using the Nextera XT Index Kit Set A, and purified again before library pooling. After quality control, final libraries were sequenced on the Illumina MiSeq using v3 reagents with a 600bp kit (paired 300-bp reads) in a single 65-hour run.