title: GSM5106587: Control_ATAC_rep2; Mus musculus; ATAC-seq
library_construction_protocol: Cells were harvested and frozen in culture media containing FBS and 5% DMSO. Cryopreserved cells were sent to Active Motif to perform the ATAC-seq assay. The cells were thawed in a 37 ˚C water bath, pelleted, and washed with cold PBS. Cell pellets were then resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera DNA Library Prep Kit (Illumina, San Diego, CA). Tagmented DNA was purified using the MinElute PCR purification kit (QIAGEN, Dusseldorf, Germany), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter, Brea, CA). The resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Wilmington, MA) and sequenced with paired-end 42-nt sequencing on the NextSeq 500 sequencer (Illumina).