title: GSM5554206: E4BP4_CM-RevDKO_ZT22.Rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Dual-crosslinked heart cells from 3xHA-Rev-erba or aMHC-Cre(+vs-)/Rev-erba/b ff/ff mice were used for ChIP pull down by anti-HA Magnetic Beads (Thermo Fisher Scientific, #88837) or anti-E4BP4 (CST, #14312). Immunoprecipitated DNA was subjected to Tn5 (Illumina, 20034197) tagmentation, and PCR amplification (Illumina, #FC-121-1031). PCR products were subjected to SPRIselect (Beckman coulter, #B23318) for size selection and pair-end sequencing on Illumina Hiseq platform.