title: GSM4782079: KO male BM donor_42; Mus musculus; RNA-Seq
library_construction_protocol: Cells isolated by laser capture microdissection were pooled in a tube. Rna was isolated and purified using the PicoPure kit. DNAse was used. RNA samples with an RNA integrity number above six were amplified using the Automated Nugen Ovation Trio Low Input RNA kit (Nugen, Manchester, UK). Samples were sequenced using the Illumina HiSeq2500 Single-end (Illumina, San Diego, CA) and the results were analyzed using Gene Set Enrichment Analysis (GSEA, Broad Institute Inc., MIT, Cambridge, MA). The NYU Genome Technology Center constructed the libraries followed their standard protocol