title: GSM5599545: Gasterosteus_f55_dorsal_fin_3sp_rep12; Gasterosteus aculeatus; RNA-Seq
library_construction_protocol: Tissues were dissected from fish and flash frozen in liquid nitrogen and stored at -80 degrees. The tissues were homogenized in a MP FastPrep with Matrix M. RNA was extracted with Takara NucleoSpin RNA XS kit with an on-column DNase step. 20-100 ng of RNA was used to make sequencing libraries with Illumina Stranded mRNA Prep kit according to manufacturer's instructions. PCR cycle number was determined by qPCR.