title: Nanopore reads for SttruQGU27
design: For long reads sequencing, a BluePippin system (Sage Science, Inc., USA) was used to fractionate the DNA template. Then we used the native barcode kit EXP-NBD104 in conjunction with the ligation sequencing kit SQK-LSK109 (Oxford Nanopore Technologies, Ltd., UK) to build a 10 kb template library. The 10 kb library was qualified using an Agilent 2100 bioanalyzer (Agilent Technologies, USA) and finally sequenced on a Nanopore PromethION 48 platform (Oxford Nanopore Technologies, Ltd., UK).