title: GSM5958155: 0.1mM MMNK-1 rep3; Homo sapiens; RNA-Seq
library_construction_protocol: PolyA RNAs were isolated from the cell lysates, using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, MA) conjugated with biotin-3' WTA-EcoP-dT25 and amplified according to the previous report with some modifications (GSE110711). 100 ng of the whole-transcriptome library was subjected to fragmentation/end-repair/A-tailing using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs Inc., Tokyo, Japan) with some modifications. Standard illumina protocols were followed to generate RNA libraries.