title: GSM5425192: 0hr-WT-5pGEX; Mus musculus; RNA-Seq
library_construction_protocol: Naive CD4 T  cells or activated CD4 T cells were collected and washed with cold wash buffer, composed of DPBS, with 1% bovine calf serum and 1% penicillin-streptomycin, and stained for 30 min on ice with antibodies. Cells were stained with anti-mouse CD4-FITC, CD3-PE and 7-AAD. 7-AAD-negative, CD3-positive and CD4-positive live immune cells were sorted with FACS Aria. library were constructed with Chromium Next GEM Single Cell 5p RNA library v1.1 (10x genomics) following manufacturer's instruction.