title: GSM5899826: PC3; Homo sapiens; RNA-Seq
library_construction_protocol: prostate cancer organoid and cell line: RNA extraction was performed according to manufacture's instuction using RNeasy Plus Mini Kit (Qiagen #74134) PDX: Tissue was coarsely pulverized on dry ice, and RNA extraction was performed per tissue protocol using the RNeasy Plus Mini Kit (Qiagen 74134). RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA kit, with 8 cycles of PCR amplification, starting from 500 ng of total RNA, at the Integrative Genomics Operation (IGO) at MSKCC