title: GSM5792388: Six1-KO rep1; Mus musculus; RNA-Seq
library_construction_protocol: E10.5 mouse embryos were obtained and the otic vesicles were removed with dissection tweezers. Total RNA from pools of vesicles (3 pools with 6 vesicles each for each genotype) was obtained using the Rneasy Micro Kit (Qiagen). Residual DNA was removed using the Turbo DNA Free Kit (Thermofisher). 10-30 ng of total RNA were used per sample for library preparation. Libraries were constructed using the QuantSeq 3' mRNA-Seq Library Prep Kit (Illumina), following the manufacturer's instructions.