title: GSM5467430: B16 PB-4 3 miRNA; Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for miRNA purification. miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by Gel electrophoresis. RNA libraries were prepared for sequencing using standard Illumina protocols