title: Illumina HiSeq 4000 paired end sequencing; Transcriptomic profiling of patient-derived endometrial cancer organoids (Berg HF)
design: Transcriptomic profiling of patient-derived endometrial cancer organoids (Berg HF)
library_construction_protocol: Organoids were dissolved from Matrigel using Cell Recovery Solution. Cells were lysed in RLT plus lysis buffer/10% β-mercaptoethanol and homogenized using a tissue lyser.  RNA was isolated using AllPrep DNA/RNA mini kit (QIAGEN) according to the manufacturer's protocol.  cDNA libraries were prepared using Illumina TruSeq Stranded Total RNA GOLD (350 ng RNA input).