title: RNA-seq of Rattus_norvegicus:  adult  male brain
design: Total RNA was extracted from the ipsilateral ischemicbrains for RNA-seq analysis using Trizol Reagent (Invitrogen, 15596018). RNA concentration was quantified by Qubit 2.0 fluorometer (Invitrogen) with Qubit RNA BR assay kit (Invitrogen, Q10211). RNA quality control (Agilent,1309) was performed using Agilent 2100 Bio-analyzer (Agilent Technologies, Inc. CA, USA) according to the manufacturers protocols