title: RNA-seq of Homo Sapiens FFPE blocks
design: RNA was isolated using the RNeasy FFPE kit (Qiagen, Catalog No-73504) from BPH and malignant Formalin-Fixed Paraffin Embedded (FFPE) blocks. From an approximate 0.5 mg of cross section, the RNA was prepared wherein the NEBNext Ultra II Directional RNA Library Prep Kit was used for preparing the libraries following the manufacturers protocol. While 100ng of FFPE RNA was used as input, it was then subjected to end repair and Illumina specific adaptors were ligated. The adaptor ligated product was then barcoded and subjected to 15 cycles of PCR. The samples after PCR were cleaned up using AMPure XP beads with the final libraries checked for quality using Qubit Fluorometer and Agilent Tapestation.