title: GSM5105258: Control_PCM1sort_Atria_3 (RNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: Isolated nuclei from both atria pooled together per individual were stained for PCM1 (pericentreolar material 1). Samples were FACS-sorted and gated in such a way that debris, nuclei clumps, and nuclei with no cardiomyocyte origin were excluded. Nuclei were collected and successively washed with PBS, RNA was extracted using ReliaPrep RNA Miniprep System (Promega) and used as input for library preparation according to protocol. Libraries were sequenced on an Illumina Hiseq4000. Ovation RNA-seq System V2 (M01206 v9, NuGen