title: GSM5700378: hop[Tum] rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: In total, three biological replicates were prepared with each biological replicate consisting of 20 pooled larvae. RNA was extracted from whole larvae using Trizol (Thermo Fisher Scientific) followed by QIAGEN RNeasy Micro clean-up (QIAGEN; both according to manufacturer's instructions). RNA concentration was measured using a nanodrop and the integrity was verified using agarose gel electrophoresis. Libraries with 250-300bp insert size were prepared for sequencing using standard Illumina protocols