title: GSM5553854: PRO-seq_MEF_HSF1-KO_C_Rep2; Mus musculus; OTHER
library_construction_protocol: Cells were washed with PBS after treatments. Next, nuclei were isolated and run-on reactions were performed in the presence of biotinylated nucleotides as described by Mahat et al., Nat. Protocol 11, 1455–1476 (2016). An equal amount of Drosophila S2 cells were added to run-on reactions to provide whole-genome spike-in control for sample normalization. Libraries were constructed using TruSeq small-RNA adaptors according to Mahat et al., Nat. Protocol 11, 1455–1476 (2016).