title: GSM5553801: Immunocastrated pigs (pooled RNA) [skele_5]; Sus scrofa; RNA-Seq
library_construction_protocol: At the end of the slaughterline (45 min post mortem), approx. 1 cm3 muscle samples were taken from the central part of LD at the level of the last rib and from the SSC i.e., from the central part of its m. biventer cervicis, at the level of the 4th cervical vertebra.  Muscles were sampled at the right side of the carcass, snap-frozen in liquid nitrogen and stored at –80°C until subsequent analysis. Total RNA was extracted using RNeasy plus Universal Mini Extraction Kit. Extracted RNA from 6 animal  samples (out of 12) were pooled in equal quantities for each male sex category group and muscle. cDNA sequencing libraries were generated using NEBNext Ultra RNA Library preparation Kit for Illumina.