title: GSM5106488: Normal human nasal epithelial cells (S. epidermidis); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from NHNE cells which were inoculated with S. epidermidis at day one using TRIzol. cDNA was synthesized from 3 μg of RNA with random hexamer primers using Moloney murine leukemia virus reverse transcriptase. Library construction was performed using 10X Chromium Single Cell 3' reagent kits v3.1. Samples were sequenced using the Illumina NovaSeq 6000 platform, and preliminary sequencing results were converted to FASTQ files using the Cell Ranger pipeline. Single cell RNA-seq