title: GSM4781956: E12.5 Tal1cko R1 1; Mus musculus; RNA-Seq
library_construction_protocol: Tissue from the E12.5 ventral r1 of 6 Ctrl and 6 Tal1cko embryos were dissected in DMEM. Total RNA was isolated by Trizol Reagent/chloroform extraction using Direct-zol™ RNA MiniPrep columns (Zymo research, catalog # R2051) followed by DNase I digestion, according to manufacturer's instructions. The RNA concentrations was measured spectrophotometrically using NanoDrop2000 (Thermo Scientific). RNA quality was assayed on RNA Nano Chips (Agilent) with Agilent Bioanalyzer 2100 according to manufacturer's instructions. All samples used for RNA-seq had RIN values of at least 9.4 Sequencing libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation Kit  (Illumina) with Ribo-Zero rRNA depletion. The libraries were sequenced in one run with the Illumina NextSeq500 sequencer producing 400 million single reads of 86 base pairs.