title: Illumina NovaSeq 6000 paired end sequencing
design: Experimental sample, transgenic
library_construction_protocol: Total RNA was quantified using RiboGreen (Invitrogen) on the FLUOstar OPTIMA plate reader (BMG Labtech) and the size profile and integrity analysed on the 2200 or 4200 TapeStation (Agilent, RNA ScreenTape). RIN estimate the sample was between 9.5 and 10. Input material was normalised to 100 ng prior to library preparation. Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturerâ€™s instructions. Library was amplified (14 cycles) on a Tetrad (Bio-Rad) using in-house unique dual indexing primers (based on DOI: 10.1186/1472-6750-13-104). The library was normalised using Qubit, and the size profile was analysed on the 2200 or 4200 TapeStation. Following normalisation, the library was pooled with other libraries for a subsequent sequencing.