title: GSM5105625: CrePos.g5.rep1; Mus musculus; RNA-Seq
library_construction_protocol: 72 hours post transduction, transduced cells were FACS purified using an SH800 and fluorescent antibody targeting Thy1.1 present in the gRNA cassette. Cell pellets were processed using Qiagen RNeasy RNA extraction kit. RNA-seq libraries were built from rRNA depleted mRNA Library preparation was performed using the Illumina TruSeq stranded mRNA prep kit (20020595)