title: Consistent patterns of microbial diversity, prediction accuracy and associated bacterial organisms between pediatric ulcerative colitis and healthy children using 98S rRNA and metagenomic shotgun sequencing data
design: The 16S bacterial DNA V4 region was amplified from the extracted DNA by PCR and sequenced in the Illumina MiSeq Reagent Kit v2 flowcell on an Illumina MiSeq System using 2 * 150bp paired-end protocol. All samples were sequenced using uniquely barcoded primers (515FB: 5-GTG YCA GCM GCC GCG GTA A-3; 806RB: 5-GGA CTA CNV GGG TWT CTA AT-3) that were modified from the original 515F-806R primer pairs. Agilent High Sensitivity DNA Bioanalyzer chips was used to assess the library qualities.