title: Sample_D2
design: total RNA was extracted and RNA integrity was evaluated by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara,  CA, USA). The samples of RNA Integrity Number (RIN) 7.0 were subjected to the subsequent analysis. Secondly,1 g of total RNA was used to construct small RNA library based on TruSeq Small RNA Sample Prep Kits (Illumina, United States). Briefly, adapters were linked to both ends of total RNA, cDNA was reverse transcribed from these and PCR amplification was performed. Pieces between 147 and 157 nt were separated from the PCR product and purified to establish a small RNA library