title: GSM5107429: Donor 2- RK-33 treated- 4 hours; Homo sapiens; RNA-Seq
library_construction_protocol: Three million primary CD4+ T cells for Donor 1 and Five million primary CD4+ T cells from Donor 2 were collected 4 hours and 18 hours post treatement with DDX3 inhibitors RK-33 and FH-1321 in TRI reagent and RNA was extracted according to manufacturer's protocols. RNA libraries were prepared for sequencing using the Illumina TruSeq Stranded mRNA Library Prep Kit. The resulting DNA libraries were sequenced according to the Illumina TruSeq Rapid  v2 protocol on an Illumina HiSeq2500 sequencer.