title: GSM4782562: Perturb-seq1; Mus musculus; RNA-Seq
library_construction_protocol: Each single-cell library was made by combining the BFP+ cells from 1-3 litters (4-20 animals) of P7 animals harvested on the same day. The FACS-purified cells were sorted into cold Hibernate A/B27 medium and subjected to single-cell RNA sequencing library preparation. Single-cell RNA sequencing libraries were created using the Chromium Single Cell 3' Solution v2 kit (10x Genomics) following the manufacturer's protocol. We used the Chromium Single Cell 3' Solution v2 kit (10x Genomics) to construct all the libraries, which are experimental replicates for the pooled Perturb-Seq.