title: GSM5553823: CK_H2_ChIPseq_IP; Oryza sativa Japonica Group; ChIP-Seq
library_construction_protocol: The total RNAs were extracted from the seedlings using TRIzol reagent (Invitrogen™) according to the manufacturer's instructions. The ChIP assay was performed according to Chris Bowler (Bowler et al., 2004) with some modiﬁcations. Briefly, 3 g seedling tissue were cross-linked in 1% formaldehyde by vacuum for 30 minutes. The chromatin was extracted on ice as described by Bowler method and sheared to 200-500 bp fragments by sonication. Subsequently, the DNA fragments were immunoprecipitated by homemade anti-DAHT1 polyclonal antibody. The ChIP DNA was eluted, purified, and dissolved in ddH2O. RNA-seq and ChIP-seq libraries were prepared for sequencing using standard Illumina protocols