title: GSM5420884: P18_Input_DNA_Ino80cKO_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Seminiferous tubules from P18 testes were digested with collagenase and trypsin to obtain spermatogenic cells followed by filtration through 40 micron filter. Cells were fixed in 0.3% formaldehyde at 4˚C for 30 minutes. Nuclei were isolated and chromatin was digested with micrococcal nuclease before immunoprecipitation with antibody. Libraries were created using Kapa hyperprep DNA library kit for Illumina sequencing