title: GSM5793826: scRNA-seq of patient 3 benign sample; Homo sapiens; RNA-Seq
library_construction_protocol: Paired malignant and benign 1x1 cm tissue biopsies were extracted within one hour after surgery from prostate specimens using punch needles. The biopsies were rinsed with PBS, minced into 1mm3 pieces and transferred to the 5 ml of digestion solution ((DMEM high glucose (Sigma-Aldrich) supplemented with with L-glutamine-penicillin-streptomycin (100 U/ml) (Sigma-Aldrich), 20 uL DNase (Quiagen) (75 U/ml), 2 mL Collagenase V (2 %) (Sigma-Aldrich) and Dispase II (2.5 U/mL) (Gibco)) for 45 minutes in water bath (37 °C). After tissue digestion and preparaing single cell suspension, viable cells were sorted and proceeded for single cell capture using the Chromium Controller (10X Genomics). Barcoding and library preparation was performed using Chromium Next GEM single cell 3' kit (v3.1, 10x Genomics) according to the manufacturer's recommendations. For each sample, an input of ~17,000 cells was used to finally recover 10000 cells. The resulting 3' Gene Expression libraries were sequenced on Illumina HiSeq 4000 at a sequencing depth of 50000 reads per cell.