title: GSM5231047: TOX4 KO pSPT5 CUT&Tag rep2; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag experiments were performed as previously described with minor modifications (Kaya-Okur, H.S. et al.). Briefly, 100,000 cells were used for each experiment. Cells were bound to Concanavalin A-coated beads without fixation and chromatin opening. After primary and secondary antibodies binding, pA-Tn5 transposome binding, and tagmentation, DNA was extracted by PCI. Library was constructed by PCR, NEB Phusion (M0530L) was used as protocol.