title: GSM5652534: RNA_398_CD4_EM; Homo sapiens; RNA-Seq
library_construction_protocol: One thousand cells were sorted directly into RLT buffer (79216; Qiagen) containing 1% 2-Mercaptoethanol. RNA was isolated using the Quick-RNA Microprep kit (Zymo Research; R1050). The SMART-Seq v4 Ultra Low Input RNA Kit (634894; Takara Bio) was used for synthesis of cRNA from isolated RNA, and 400 pg of cDNA was used as input for the NexteraXT kit (Illumina).