title: GSM5914861: CpOGA_WT_IP_H3K27me3; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Embryos lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibodies. And 100ng total RNA was harvested using Trizol reagent from a single embryo. The sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols.