title: GSM4732825: H9-WT+2iLAF; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Kit (QIAGEN) and quantified using a Nanodrop ND-2000 (Nanodrop). mRNA was purified from total RNA (1 μg) by oligo(dT)-coupled magnetic beads and fragmented into small pieces under elevated temperature. The first-strand cDNA was synthesized using reverse transcriptase and random primers. After the generation of double-strand cDNA and adenylation on 3' ends of DNA fragments, the adaptors were ligated and purified with AMPure XP system (Beckman Coulter, Beverly, USA). The quality of the libraries was assessed on the Agilent Bioanalyzer 2100 system and a Real-Time PCR system. TruSeq Stranded mRNA Library Prep Kit.