title: GSM5008000: GfpOn_2; Bacillus subtilis; RNA-Seq
library_construction_protocol: GFP-on/off cells were respectively broke using glass beads (0.1 mm) and RNA was extracted using 25:24:1 phenol/chloroform/isoamyl alcohol (P/C/I) mixture, and Dnase I was used to remove DNA from the RNA samples. 1 μl 1:1000 diluted ERCC RNA Spike-In mix 1 (Invitrogen) was added to 50 ng total RNA and the mixed RNA was subjected to sequencing library construction using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs)