title: GSM4781962: E12.5 control R1; Mus musculus; RNA-Seq
library_construction_protocol: Mice, their tissues and cells were untreated Ventral r1 was dissected in the 5% FBS (fetal bovine serum; Millipore, Cat#ES-009-B) in L-15 (Gibco, Cat#11415-049). The ventral r1 was separated from the brain by using visible landmarks (such as the constriction of midbrain-hindbrain boundary). The pieces were washed 3 times with cold 500ul 1xPBS (Lonza, Cat#BE17-516Q) and dissociated using the Papain Dissociation System (Worthington, Cat#LK003150) according to the manufacturer's protocols. Post-dissociation, the cells were  washed 3 times in cold PBS and counted to determine the number of apoptotic cells (1-5%) before encapsulation. InDrops E12.5 cDNA libraries were multiplexed and sequenced on NextSeq Illumina platform in paired-end mode using high-yield 75 cycle kit.