title: GSM5899813: Kid.double_pos_22; Mus musculus; RNA-Seq
library_construction_protocol: Cell pellets were  re-suspended in RLT buffer and total RNA was purified using the TNeasy Mini kit according to manufacturers instructions and RNA concentration was estimated by an agilent bio analyzer. cDNA was synthesized using Clontech Smart Seq v4 reagents from 2ng of RNA. Full length cDNA was fragmented to a mean size of 150bp with a Covaris M220 ultrasonicator and Illumina libraries were prepared from 2ng of sheared cDNA using Rubicon Genomics ThruPLEX DNAseq reagents