title: Low-input RNA-seq equine endometrium LCM samples
design: RNA-Seq library preparation starting from 2.5 ng total RNA and following the instructions of the Ovation Solo Single Cell RNA Seq System kit (NuGen Technologies, San Carlos, USA). For the first amplification step, the number of PCR cycles was between 7 and 13. Each library was barcoded individually for multiplexing (5 different multiplex pools).