title: GSM5555293: 5995-4: ded1-ref_rep4; Zea mays; RNA-Seq
library_construction_protocol: total RNA was extracted from 12 DAP normal and ded1-ref endosperms RNA was extracted with Trizol reagent (Ambion, Thermo Fisher), followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Non-strand-specific TruSeq (Illumina) cDNA libraries were  prepared from 1 mg total RNA input with a 200 bp median insert length as described in Bai et al., 2019