title: GSM5419680: RNA-seq_Pericardium replicate 1; Danio rerio; RNA-Seq
library_construction_protocol: Dorsal pericardium, proepicardium and heart-tube were manually dissected, with wolfram needles, from 60 hpf epi:eGFP;myl7:mRFP zebrafish larvae. A minimum of 10  of each tissue/organ were collected for each sample in ice cold PBS. Cells were centrifuged for 7 minutes at 250 g. The excess liquid was removed, and the cells were stored at -80oC until further use RNA was directly transformed and amplified into cDNA from the lysed tissue using the SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing – HV kit. cDNA quality control was verified using the Agilent 2200 BioAnalyzer and the High Sensitivity DNA Chip from Agilent's High Sensitivity DNA Kit