title: GSM5106525: Sertoli_PRC1Rctrl_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from Sertoli cells using an RNeasy microkit (Qiagen) according to the manual provided. For ChIP-seq, libraries were created by ChIPmentation method (PMID: 26280331).For RNA-seq, RNA quality and quantity were checked using Bioanalyzer (Agilent) and Qubit (Life Technologies), respectively. The initial amplification step was performed with the NuGEN Ovation RNA-seq system version 2. The assay was used to amplify RNA samples and create double-stranded cDNA. Libraries were then created with the Nextera XT DNA sample preparation kit (Illumina).