title: GSM5105609: mmCD4.CREneg.g5.K27ac.rep1; Mus musculus; ChIP-Seq
library_construction_protocol: 72 hours post transduction, transduced cells were sort purified using an SH800 and fluorescent antibody targeting Thy1.1 present in the gRNA cassette. Cells were crosslinked for 10 minutes at RT with 1% formaldehyde before nuclei were processed for ChIP as described in Savic et al, 2013. Briefly, following fixation, cells were incubated with 1mL Farnham Lysis Buffer for 10 minutes followed by centrifugation. Pellets were resuspended in 100µL RIPA buffer and sonicated in a 1.5mL tube for 30 cycles of 30s on and 30s off using a Bioruptor Pico. ChIP-seq libraries were prepared using a Kapa Hyper Prep kit.