title: GSM4783653: AD_GKO; Mus musculus; RNA-Seq
library_construction_protocol: Single nuclei were isolated based on the published protocol with modifications of Krishnaswami et al., 2016. After dissection, hippocampi were homogenized in a cold Dounce homogenizer after dissection. The homogenate was centrifuged at 1,000g for 8 min at 4 °C to pellet nuclei. Nuclei were resuspended in 1 ml 29% iodixanol buffer and centrifuged at 13,500 g for 20 min at 4°C. Supernatant and floating myelin were removed after centrifugation. Nuclei were resuspended in 100 µl nuclei storage buffer and filtered using a 40 µm cell strainer. Nuclei were stained with trypan blue to and counted using a hemocytometer. RNA libraries were prepared for sequencing using standard 10X Genomics protocols