title: Effects of ethanol and nitrate on ibuprofen removal in batch reactors under denitrifying conditions
design: Total genome DNA was extracted using the FastDNA SPIN Soil DNA Extraction Kit (MP Biomedical) following the manufacturer's recommendations. The integrity of the extracted DNA was verified on a 0.8% agarose gel and the quantification (ng /L) and the purity (ratio 260/280nm) were performed in the Nanodrop 2000 Spectrophotometers (ThermoFisher Scientific). Then the DNA was delivered to the company NGS Genomic Solutions in Piracicaba, SP, to carry out the sequencing. The 16S rRNA were amplified using the primer set 341F - 806R  with barcode. All PCR reactions were performed with Phusion High-Fidelity PCR Master Mix (New England Biolabs). Sequencing libraries were generated using NEBNext Ultra DNA Library Pre  Kit for Illumina, following the manufacturer's recommendations.