title: RNAseq_T.aestivum_coleoptile_98221_IEB
design: Total RNA was isolated using RNEasy Plant Mini Kit (Qiagen, Germany) and RNA quality was checked on Bioanalyzer using Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, USA). Sequencing libraries were prepared by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs,  Ipswich, MA ) and pair-end sequenced on the Illumina NextSeq550 System