title: GSM5420938: P18_RNA-seq_Ino80cKO_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Seminiferous tubules from P18 testes were digested with collagenase and trypsin to obtain spermatogenic cells followed by filtration through 40 micron filter. Cells were lysed in TRIzol reagent and total RNA was extracted by Zymo Direct-zol kit following manufacturers instruction. Libraries were generated by Kapa stranded mRNA-seq kit library kit for Illumina sequencing isong 1µg RNA per sample.