title: Metagenomic sequencing of ANME-2d enrichment
design: To shear genomic DNA and add adapters in the same step (tagmentation), the Illumina Nextera XT library prep kit (Illumina, San Diego, CA, USA) was used. The library was normalized to 4 nM and sequenced with a MiSeq instrument (Illumina) using the 300 paired-end sequencing protocol