title: GSM5104068: hda-1 RNAi embryo-rep1 (RNA-seq); Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Eggs were harvested 5 hours post-laid and then lysed with TRIzol reagent (Invitrogen). We extracted the total RNA following the manufacturer's protocol. The Qubit RNA High Sensitivity Assay Kit (Invitrogen) was used to quantify RNA concentration. The Agilent 2100 bioanalyzer system was used for the assessment of RNA quality. Samples with an RNA integrity number (RIN) above 6.0 were used for sequencing library construction. We used the identical amounts of input total RNA (50 ng to 500 ng) between control and samples for library preparation using the KAPA RNA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA). Libraries were analyzed by Agilent 2100 bioanalyzer system for quality control.