title: GSM5230187: Rh-Lung-35_POD [Rh-Lung-461-18]; Macaca mulatta; RNA-Seq
library_construction_protocol: Approximately 10 mL of amniotic fluid from each animal was used for cell-free RNA extraction with the QIAamp Circulating Nucleic Acid Kit (QIAGEN, Valencia, CA). RNA from the lung and hippocampus was extracted with the RNeasy Universal Mini Kit (QIAGEN, Valencia, CA). The RNAseq libraries were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina). RNA-Seq was performed at a target depth of 30 million reads, with paired-end 75 bp RNA-Seq performed with a HiSeq 2500 (Illumina). by the Cincinnati Children's Hospital Sequencing Core.