title: Illumina NovaSeq 6000 paired end sequencing; Whole metagenome sequencing of fecal sample.
design: Whole metagenome sequencing
library_construction_protocol: DNA was extracted using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Thermo Fisher, Catalog#: A42358) using 100 mg of fecal sample for 800 µL of Lysis Buﬀer. Bead beating was performed for 5 min at 50 Hz. Lyzate was centrifuged at 14,000g for 2 min and 400-500 ul  of supernatant was used in subsequent steps using a KingFisher Flex system (Thermo Fisher) and following the manufacturer's protocol. Extracted DNA amount was measured using the Qubit dsDNA Assay Kit (Thermo Fisher). Sequencing library was prepared with 100 ng of DNA per sample. Briefly: Shearing was performed on a Covaris LE200 system, end repair, A-tailing, ligation of adaptors and PCR were performed using the KAPA Hyper Prep Kit (Roche, Catalog#: 07962363001).