title: GSM4782993: SLE2_RNA; Homo sapiens; RNA-Seq
library_construction_protocol: The RNA-seq of monocytes were performed by the Smart-Seq2 method. Briefly samples were collected in tubes with lysis component and ribonuclease inhibitor. Then an Oligo-dT primer was introduced to the reverse transcription reaction for first-strand cDNA synthesis, followed by PCR amplification to enrich the cDNA and magbeads purification step to clean up the production. cDNAs were amplificated according to Smart-seq2 procedures for construct Illumina RNA sequencing library.