title: GSM5701282: HeLa-cas9 cells 'Unsorted' ataxin-2 with GAPDH control 2; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted for all screen populations separately according to the protocol included with QIAGEN Blood Maxi Kit. sgRNA sequences were amplified and prepared for deep-sequencing by two sequential PCR reactions as described previously (Morgens et al., 2016).