title: GSM5105295: tumor organoid MiR34aKO_2; Mus musculus; RNA-Seq
library_construction_protocol: Intestinal adenoma cells from 3-5 tumors for each ApcMin/+ mouse were isolated. 1.5 ×104 single cells per sample were embedded in matrigel and grown for 3-4 passages. RNA was harvested using Rneasy Mini Kit (Qiagen). 1 µg of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols