title: GSM5899260: ATAC_siCtrl_0Gy_rep2; Homo sapiens; ATAC-seq
library_construction_protocol: For ATAC-seq, 50,000 cells were lysed with ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween20, and 0.01% Digitonin. Nuclei were pelleted and incubated with 100 nM Tn5 transposase (Illumina, Cat#FC-121-1030) for 30 min at 37°C. Transposed DNA was Cleaned up with Zymo DNA Clean and Concentrator-5 Kit (cat# D4014). For ATAC-seq, The purified DNA were amplified for 3 cycles using NEBNext 2x MasterMix and purified with AMPure XP beads (Beckman).