title: GSM5555001: Single-cell sedentary high-fat diet rep3 [SH3.SM]; Mus musculus; RNA-Seq
library_construction_protocol: Fresh tissues were enzymatically digested and dissociated according to tissue dissociation kit protocols (adipose tissue Cat No 130-105-808, skeletal muscle Cat No 130-098-305 by Miltenyi) using the gentleMACS™ Dissociator (Miltenyi). Dissociated tissues were filtered, centrifuged, and the isolated cells were resuspended in 0.1% BSA in PBS and immediately processed for the generation of single-cell RNA (scRNA) libraries using the droplet-based RNA sequencing technology. Briefly, 5000-6000 cells were profiled per sample using the Chromium Single Cell 3' RNA reagent kit v3 according to the 10X Genomics protocol.