title: GSM5107665: 4182-Control; Mus musculus; RNA-Seq
library_construction_protocol: Leukemic splenocytes were harvested from two independent Dnmt3afl/fl,Flt3ITD/ITD,Mx1Cre (Dnmt3a+/-Flt3ITD AML) mice. Red blood cells were lysed, and 100x106 leukemia cells were frozen. Frozen cells were thawed and the treatment protocol was performed. At the end of the treatment protocol cells were lysed with Trizol. RNA was extracted according to Meyer et al., 2009. RNA quality and quantity were measured by Agilant 2200 Tape Station. Then samples with a RIN>8.0 were processed by ribosome depletion, stranded True-seq, paired-end 150 base pair (bp), 30 million reads. RNAs were detected by RNAseq analysis using paired-end 150 nucleotide (nt) sequencing and 30 million reads for all samples. RNA Seq libraries were generated with Illumina TruSeq Stranded mRNA HT kits.