title: RNA-seq of Foxtail millet (Setaria italica) genotype IC4/ IC403579 under drought (24 hr PEG treatment)
design: The Seeds of dehydration and salinity tolerant IC-403579 (IC4) and sensitive IC-480117 (IC41) cultivars of foxtail millet were sown in a plant growth chamber with the following conditions; 281C day/ 231C night/ 705% relative humidity with a photoperiod of 14 hr and a photosynthetic photon flux density of 500 mol m-2 s-1. For dehydration stress, twenty-one days old seedlings were treated with 20% polyethylene glycol 600 (PEG-6000). The dehydration stressed samples were collected at 24 hr after PEG treatments. The untreated samples were also collected as controls (0hr). Total RNA was extracted from the seedlings of 24hr PEG-treated and untreated control (0hr) samples using Spectrum plant total RNA Kit (Sigma Aldrich, St. Louis, MO, USA). The 10g of total RNA was treated with DNase (Ambion, Foster city, California, USA). The 5g total RNA (DNase-treated) was outsourced for the RNA-Seq on HiSeq 2000 genome analyzer (Illumina) sequencing platform. The paired-end RNA-Seq was performed for all the samples. Three biological replicates were sequenced for each 0 and 24hr PEG treatments.