title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of human iPSC-derived neurons CRISPR-engineered to express a Q331K point mutation in the TARDBP locus and comparison to isogenic controls
design: RNA-seq of human iPSC-derived neurons CRISPR-engineered to express a Q331K point mutation in the TARDBP locus and comparison to isogenic controls
library_construction_protocol: Bulk RNA samples from WTC11 cells were isolated using the Trizol reagent and according to the manufacturers' protocol. Bulk RNA samples from WTC11 cells were isolated using the Trizol reagent and according to the manufacturers' protocol. Quality of the isolated RNA was confirmed using TapeStation; only samples with an RNAi value of 8.5 or greater were used for library preparation.  Illumina Hiseq workflow