title: GSM6281179: CJ52F; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen samples from liver metastases of CRC retrieved surgically from 2009 to 2019 were used for mRNA extraction according to Qiagen Kit Qiagen AllPrep DNA/RNA Mini Kit (ref 80204manufacturer's instructions. The concentration of each library was measured by real-time PCR, samples with RIN score <7 according were discarded. Constructed libraries were sequenced (200 cycles) in paired-end (2x100 bp) using a Novaseq 6000.