title: Metagenome of marine wate
design: DNA was extracted using the Diatom DNA prep kit from the bacterial fraction of marine water (3L) sattled on 0.22 m Sterivex filter. DNA was extracted twice with phenol-chloroform and chloroform and precipitated in ethanol for the additional purification. V3-V4 region of 16S rRNA gene was amplified using standard degenerate primers fused with sequencing adapters (see Illumina guide for 16S Metagenomic Sequencing Library Preparation, Part # 15044223 Rev. B): Forward primer = CCTACGGGNGGCWGCAG, Reverse primer = GACTACHVGGGTATCTAATCC, adapter sequences are not shown.