title: GSM5792568: donor 4_unstim; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen cell pellet from each well was lysed by addition of 350 µl of the TRI Reagent solution included in the Direct-zol-96 RNA kit. Total RNA was then isolated from the lysates using the Direct-zol-96 RNA kit. 60 ng of total RNA was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext rRNA Depletion Kit (New England Biolabs). The libraries were paired-end sequenced (2 × 75 bp) on NovaSeq 6000 instrument (Illumina) using the NovaSeq 6000 S2 Reagent Kit (200 cycles).