title: GSM5899866: sal_STI150-mCh-K1_K9m3; Drosophila melanogaster; OTHER
library_construction_protocol: CUT&RUN was performed using a modified version of a Drosophila specific CUT&RUN protocol (Ahmed, 2019). Approximately 100 pairs of dissected L3 larvae salivary glands were utilized for Cut&Run per genotype. CUT&RUN sequencing libraries were prepared using the NEXTFLEX RAPID DNA-seq Kit 2.0 (Bioo NOVA 5188-12) following the manufacturer's protocol with modification. Briefly, the thermal cycler program for End-Repair and Adenylation was modified (30min at 20C, 75 min at 58C), adapter ligation proceeded at 8C overnight, and post-ligation NEXTFLEX bead clean-up was not size selective to retain small fragments.