title: ITS-Seq of red mud
design: ITS4 (5'- TCCTCCGCTTATTGATATGC-3') and  gITS7F (5'-GTGARTCATCGARTCTTTG-3').The PCR mixture (25 ul) contained 2x Taq MasterMix 12.5ul, Forward primer 1ul, Reverse primer 1ul, ddH2O 9.5ul, DNA(10ng/ul) 1ul. The PCR amplification program included initial denaturation at 94 C for 5 min, followed by 34 cycles of 94 C for 30 s, 56 C for 30 s, and 68 C for 45 s, and a final extension at 72 C for 10 min. Conduct two PCR reactions for each sample, and combine them together after PCR amplification.