title: GSM5420952: KO_PBS_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested via Trizol reagent, followed by phenol/chloroform separation. Aqueous phase was purified by Qiagen Total RNA mini kit according to manufacturer protocol. Poly-A mRNA was purified followed by fragmentation, and first and second strand cDNA synthesis with Illumina mRNA TruSeq library preparation protocol. Double stranded cDNAs were processed for TruSeq dual-index Illumina library preparation. Pooled multiplexed libraries were sequenced on a HiSeq 2500 machine on rapid mode at 30-40 million 50bp paired-end reads per sample.