title: GSM5172392: THP-1_control; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted from the co-cultured THP-1 cells, or FACS-sorted cells from the tumor and non-tumor liver tissues using the RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer's instructions. The qualities of total RNAs were measured by spectrophotometer (NanoDrop, Thermo Fisher, USA). Libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module kit (NEB, USA) and NEBNext Ultra RNA Library Prep Kit for Illumina Paired-end Multiplexed Sequencing Library (NEB, USA). Samples were sequenced on the Illumina Hiseq 4000 sequencer with 150 bp paired-end reads.