title: GSM5104956: ZsYellow negative cells - replicate 1; Danio rerio; RNA-Seq
library_construction_protocol: Trizol lysis, chloroform extraction and column-based purification (Qiagen RNeasy Kit). RNA sample quality was evaluated using a 2100 Bioanalyzer Instrument (Agilent Technologies). Approximately 50ng of RNA per sample were used to prepare sequencing libraries with the Clontech low-input RNA library preparation kit. Libraries were sequenced as paired-end 2x50nt on an Illumina HiSeq2500 instrument. polyA-based RT-priming followed by tagmentation-based RNA-Seq protcol