title: Acanthamoeba polyphaga infected with Mimivirus 1,3,5h post infection as well as uninfected mix
design: PacBio Single Molecule Real-Time (SMRT) Sequencing of long reads- RNA was extracted from AP infected with Mimivirus 1 h, 3 h and 5 h PI, as well as uninfected and 1 h post UV radiation. We used SMARTer PCR cDNA Synthesis Kit (TaKaRa ,cat 634926) and  PrimeSTAR GXL DNA Polymerase (TaKaRa, cat R050B) for cDNA preparation. Libraries were prepared using the SMRTbell Express Template Prep Kit 2.0. Sequencing was done using a SMRT cell 1M V3 on the Sequel instrument using sequel sequencing plate 3.0