title: 16S metagenomic sequence of water sample:  pre storm summer sample at station J76-replicate
design: Surface water samples were collected with a bucket lowered from an overpass or dock. About 75-300ml water were pulled through a Sterivex SVGPL10RC 0.2 m cartridge until refusal. Microbial community DNA was recovered from the Sterivex cartridges. These crude extracts were subsequently purified by passage through a OneStep PCR Inhibitor Removal column. The V4 region of the 16S rRNA gene was amplified to generate an amplicon library.