title: RNA-seq of serum of healthy controls pool 2
design: Total RNA was extracted from serum using the miRNeasy Micro kit (Qiagen). 1ng of extracted RNA per sample were combined into age- and sex-matched pools of four to five samples each and concentrated using a SpeedVac at ambient temperature for 40 min from 50 uL to 8 uL. Each sample pool was then converted into RNA-Seq libraries using the QIAseq miRNA library kit (Qiagen) following the recommended parameters for serum RNA. These libraries then underwent 75 bp paired end sequencing on an Illumina NextSeq 500 machine at the University of Leeds, United Kingdom.