title: 10x Chromium linked read WGS of Cooinda the Alpine Dingo: blood
design: Genomic DNA was prepared from fresh blood using the genomic-tip 100/G kit (Qiagen, Hilden, Germany) with supplemental RNase and proteinase K treatment. Isolated genomic DNA was further purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) and size selected on Sage BluePippin gels (Sage Science, Beverly, MA, USA). A 10X GEM library was barcoded from high molecular weight (HMW) DNA according to the manufacturer's recommended protocols. The library was run on a single lane of a v2 patterned flowcell. Paired-end sequencing with 150-bp read length was performed using the Illumina HiSeq X.