title: GSM5466076: AB1_PC_3; Mus musculus; RNA-Seq
library_construction_protocol: At day 10 after tumour inoculation, mice were euthanized and the tumours and surrounding tissues were harvested. Tumours were submerged in RNAlater (Life Technologies) at 4 C overnight to allow RNAlater to penetrate the tissue. Then, tumours were removed from RNAlater and stored at –80 C until dissociation with TRIzol (Life Technologies) using a TissueRuptor (QIAGEN). RNA was extracted with chloroform and purified on RNeasy MinElute columns (QIAGEN). Library preparation and sequencing at 50 bp single end reads with Illumina HiSeq standard protocols were performed by Australian Genome Research Facility.