title: GSM6249161: Callus, GP, rep2; Hordeum vulgare; RNA-Seq
library_construction_protocol: Total RNA was isolated from 31-day-old calli sample (28 days BCIM dark + 3 days BRM indirect light) of GP and D91  using RNeasy plant RNA isolation kit with on column DNase digestion (Qiagen). 1.5 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.