title: UCE target enrichment of Allorhogas_uberlandiensis_CNIN4122: whole organism
design: DNA was extracted from voucher specimens following a nondestructive technique  using the EZ-10 Spin Mini preps Kit (Bio Basic, Toronto, Canada). We followed Branstetter et al.s (2017) protocol for library preparation and capture and enrichment of UCE loci. All DNA extractions were quantified using Qubit 2.0 (Life Technologies). For each sample, up to 50 ng of extracted DNA was fragmented to an average fragment distribution of 400600 bp using either a Bioruptor Pico sonication device (Diagenode Inc., Danville, NJ, USA) or a Qsonica Q800R sonicator (Qsonica LLC, Newton, CT, USA). Genomic libraries were constructed using the Kapa Hyper Prep library preparation kit (Kapa Biosystems Inc., Wilmington, MA, USA) and TruSeq-style dual-indexing barcodes (Glenn et al., 2019). Target enrichment of the DNA libraries was performed  using the two enhanced commercial myBaits UCE sets (1.5Kv1 and 2.5Kv2; ArborBiosciences, Ann Arbor, MI, USA) that were specifically designed for the order Hymenoptera and developed by Faircloth et al. (2015) and Branstetter et al. (2017). Pooled enriched libraries were sent to two different locations for sequencing services. Sequencing was performed for a first pool with an Illumina NextSeq v2 300 cycle kit (150-bp pairedend, Illumina Inc., San Diego, CA, USA), whereas the remaining enriched libraries were sequenced using an Illumina NovaSeq 6000 (150-bp paired-end, Illumina Inc., San Diego, CA, USA).