title: C. jejuni-MBS3434A
design: Isolates were grown from frozen glycerol stocks by streaking for single colonies on 5% horse blood agar. Plates were incubated for 24 to 48 h at 42C under microaerobic conditions (10% CO2, 5% O2, 85% N2). DNA was extracted from single colonies using the EZ1 DNA tissue kit (Qiagen) according to the manufacturers instructions. Genomic DNA was quantified using the Quant-it High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON) and DNA libraries were prepared from 1 ng of genomic DNA with Illumina Nextera XT DNA sample preparation kit (Illumina, Inc., CA, USA) according to manufacturers instructions. Whole-genome sequencing was performed on the Illumina MiSeq platform (Illumina Inc.), using a 600 cycle MiSeq reagent kit (v3) generating 2  300 bp paired-end reads.