title: GSM5172158: sample09_donor8; Homo sapiens; RNA-Seq
library_construction_protocol: RNAseq: RNA was exracted using the Rneasy Mini Kit (QIAGEN, Hilden, Germany). ChIPseq: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. RNAseq:Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA) and subjected to sequencing on 1-2 Illumina HiSeq 2000 lanes to obtain an average of approximately 100-150 million uniquely mapped reads for each sample. ChIPseq: ChIP-DNA was treated with RNase A (Qiagen) and proteinase K (ThermoFisher Scientific) and then purified. The ChIP-DNA samples were end repaired and A-tailed. The Illumina TruSeq adapters (Illumina, San Diego, CA) were ligated and size-selected from the gel before PCR amplification. PCR products were purified and size selected in the gel again. The final purified samples were sequenced on HiSeq4000 (Illumina).