title: GSM5311688: WT_Rep2; Oryza sativa; RNA-Seq
library_construction_protocol: To construct sRNA libraries, 30 ug of total RNAs was extracted from seeding shoots using TRIzol® Reagent (Invitrogen, USA). After RNAs were resolved on a 15% urea PAGE, gel slices containing 15-40 nt RNAs were recovered, smashed and placed in 500 μL 0.4 M NaCl solution. The resulting mixture was agitated at 30 rpm at 4 °C overnight followed by supernatant collection and sRNA recovery as described previously SRNA libraries were constructed using the Next Multiplex Small RNA Library Prep kit (NEB, USA) following the manufacturer's instructions and sequenced on an Illumina HiSeq2500 platform with the SE50 sequencing strategy at Berry Genomics Co., Ltd.