title: GSM5311509: m1_primary_tumor2; Mus musculus; RNA-Seq
library_construction_protocol: Tumor cells were sorted using FACS directly into RNAprotect. RNA was extracted using the RNeasy micro kit (catalog #74004, Qiagen), according to manufacturer's instructions. At least 300 ng RNA was shared for input. Library preparation, sequencing, and initial quality control was performed by Novogene (Beijing, China). Briefly, mRNA was enriched using oligo(dT) beads followed by library generation using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB), according to manufacturer's instructions. To allow for sequencing different samples at the same time, adaptor sequences were added to each library. RNA sequencing; NEBNext® UltraTM RNA Library Prep Kit for Illumina®