title: GSM5172271: control cow mammary, 23C; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNeasy Plus Mini Kit total RNA samples were depleted for ribosomes using QIAseq FastSelect –rRNA HMR kits RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina by following the manufacturer's recommendations (NEB, Ipswich, MA, USA) The sequencing libraries were multiplexed and clustered on two lanes of a flowcell and loaded on the Illumina HiSeq instrument according to manufacturer's instructions.  The samples were sequenced using a 2x150 paired end configuration.