title: GSM5104785: cht7_2_ND36; Chlamydomonas reinhardtii; RNA-Seq
library_construction_protocol: Total RNA was extracted with Rneasy® Plant Mini Kit (cat. 74903, Qiagen). The RNA concentration was measured with Qubit® RNA BR Assay and Fluorometer (Cat. Q10210 and Cat. Q32866, Thermo Fisher Scientific). The quality of RNA was determined with Agilent 2100 Bioanalyzer and Agilent RNA 6000 Pico Assay (Cat. G2939BA and Cat. 5067-1514, Agilent Technologies) to ensure an RNA Integrity Number (RIN) value ≥8 before submitting an average of 4.36 µg of total RNA (220 ng/µl) per sample for RNA sequencing. Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit on a Perkin Elmer Sciclone G3 robot following manufacturer's recommendations. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS and Advanced Analytical Fragment Analyzer High Sensitivity DNA assays.