title: Deep WES Sequencing of cell-free DNA: adult
design: Peripheral blood samples were collected in K2EDTA or Streck Cell-Free DNA BCT tubes and processed according to local standards to isolate plasma before freezing. Following centrifugation, plasma was stored at -80C until cfDNA isolation. Cell-free DNA was extracted from 2 to 16 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen) according to the manufacturers instructions.