title: GSM5899493: Col_3h_30FR_1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: The seedlings (~ 25 seedllings) were collected directly in the described condition and snap-frozen in liquid nitrogen. Libraries were prepared according to Illumina's instructions accompanying TruSeq Stranded mRNA Library Prep Kit. Briefly, total RNA (0.5µg) from each sample was used to isolate poly(A) mRNA. Steps involved in library preparation were mRNA fragmentation, first strand cDNA synthesis followed by second strand cDNA synthesis and adaptor ligation, cDNA fragments isolation and amplification. 