title: GSM5106959: RNA-seq_CD24_CD29_LFD_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Femurs were isolated from mice, subjected to mechanical and enzymatic digestion, CD24+CD29+ skeletal stem cells were then isolated by FACS. RNA was extracted from lysed cells using the RNeasy plus microkit from Qiagen (Cat #74034) cDNA libraries were generated using SMART-Seq v4 Ultra low input RNA plus Nextera XT DNA sample preparation kit and sequenced on an Illumina Nova Seq 6000 sequencer (pair end 50)