title: Illumina HiSeq 4000 paired end sequencing; Bulk ATAC-seq experiments on human samples from normal gastric cardia, normal oesophageal submucosal glands and Barrett's oesophagus tissue
design: Bulk ATAC-seq experiments on human samples from normal gastric cardia, normal oesophageal submucosal glands and Barrett's oesophagus tissue
library_construction_protocol: NG samples 1-6 were obtained from the normal gastric cardia of surgical resections deposited in the Manchester Cancer Research Centre Biobank (REC: 07/H1003/161+5) and were accessed through an approved project (REF: 13_DAGI_05). BE5 and SMG 1-4 were obtained either by endoscopy at Cambridge University Hospitals NHS Trust (REC 15/EE/0152) or from deceased transplant organ donors (REC 15/EE/0152) at the time of organ donation. Nuclei were extracted from tissue samples as described previously (PMID: 28859074).  Approximately 50,000 nuclei, or all extracted nuclei if fewer, underwent transposition reaction as per the Omni-ATAC-seq protocol described previously (PMID: 28846090).