title: GSM4827544: E9.5 and E10.5 Baf155 KO yolk sac; Mus musculus; RNA-Seq
library_construction_protocol: Yolk sacs were dissected from equal numbers of mouse E9.5 and E10.5 embryos per genotype, mixed together and dissociated with 0.25% collagenase at 37°C for 30 min. The cells were stored at −80°C in fetal bovine serum with 10% DMSO. When the 10x Genomics instrument was ready, the cells were thawed and washed, and stained with antibody against Ter119 (Ly76) (1:200, 12-5921-82, eBioscience). Dead cells and Ter119+ cells were excluded by sorting to enrich live non-erythroid cells. Single cell suspension at 300 cells/mL in PBS were subjected to Chromium 10X Genomics library construction.The generated scRNAseq libraries were sequenced using a HiSeq2500 sequencer.