title: NextSeq 500 sequencing; RNA-seq of mouse embryonic stem cells with Ronin/Thap11 knockout
design: RNA-seq of mouse embryonic stem cells with Ronin/Thap11 knockout
library_construction_protocol: Cells were collected from culture using standard procedures.  Mouse embryonic stem (ES) cells were maintained in the presence of 4 ng/ml Lif, on plastic dishes coated with mitotically inactivated mouse embryonic fibroblasts.  Total RNA was isolated using Macherey-Nagel RNA isolation kit. 1ug of this was used for library preparation and sequencing. Enrichment of mRNA was performed using NEBNext® Poly(A) mRNA Magnetic Isolation Module and cDNA library was constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit.