title: ITS amplicon metagenomics of TX grad soils
design: Soil DNA was extracted with MoBio PowerSoil DNA Isolation Kits in duplicate 0.5 g subsamples and pooled. Gene amplification of 10 ug uL-1 DNA was performed in triplicate using the ITS1f (5 CTTGGTCATTTAGAGGAAGTAA 3) and ITS2 (3 CGTAGCTACTTCTTGCGTCG 5) fungal primers and pooled for sequencing on MiSeq v2, 2x250 PE at the University of Texas Genome Sequencing and Analysis Facility (Austin, TX).