title: GSM4732736: C.elegans L3_Cd 20_rep3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: The RNA isolation followed the standard procedures using the Quick-RNA Kit (Zymo Research), however modified to include a homogenization step with 0.5 mm glass beads (Sigma) to maximize cell breakage. Libraries were prepared according to Illumina's instructions. Briefly, mRNAs were purified using Poly(A) selection from total RNA and then fragmented. First strand of cDNA was synthesized using random priming, followed by the synthesis of the second strand of cDNA. The resulting double-strand cDNA was end repaired, phosphorylated and A-tailed. Adapter ligation and PCR amplification were performed, rendering the library ready for Illumina flow cell clustering and sequencing on an Illumina HiSeq following the manufacturer's protocols.