title: GSM5107393: A1_Clean_Data1; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA was extracted using TRIzol reagent. RNA-Seq was conducted according to a previously reported method. Briefly, after quality control and purification, the total RNA from each sample was used to enrich mRNA. The mRNA was fragmented and reverse-transcribed to cDNA. The cDNA was then purified, linked with the adaptor, size selected, and PCR amplified to obtain the cDNA library. After the quality check, libraries were sequenced through the Illumina platform. RNA libraries were prepared for sequencing using standard Illumina protocols