title: Ion Torrent Proton sequencing; Assessing the impact of XAB2 on gene expression and pre-mRNA splicing
design: Assessing the impact of XAB2 on gene expression and pre-mRNA splicing
library_construction_protocol: HEPA cells were cultured in standard medium containing Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 50Î¼g mL-1 streptomycin, 50 U mL-1 penicillin (Gibco), 2mM L-glutamine (Gibco). Total RNA was isolated from cells using a Total RNA isolation kit (Qiagen) as described by the manufacturer. Standard Ion Total RNA-Seq Kit v2 for Whole Transcriptome Libraries protocol