title: GSM5311443: FLAG-METTL5 IP 1; Homo sapiens; RIP-Seq
library_construction_protocol: Cell pellets were lysed, lysate was clarified, and then anti-FLAG immunoprecipitation was performed to isolate FLAG-METTL5. Samples were digested with Proteinase K and then RNA extracted using Trizol followed by choloroform extraction and isopropanol precipitation Sequencing libraries were prepared using the TruSeq Stranded mRNA library preparation kit (Illumina), using 30-40 ng of RNA for each input and IP sample.