title: NextSeq 500 paired end sequencing; PRMT5 genomic deregulation supports growth and stress tolerance in chronic lymphocytic leukemia
design: PRMT5 genomic deregulation supports growth and stress tolerance in chronic lymphocytic leukemia
library_construction_protocol: Cell lines were taken from the stock of the cell lines bank (Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures). Cell lines were authenticated by DNA profiling and cytogenetics. HG-3 and PGA-1 cells were treated with 10 ÂµM EPZ015666 for 96 h or incubated with a similar volume of DMSO as control, prior to isolation of total RNA for sequencing Total RNA was extracted via miRNeasy Mini Kit (Qiagen, Hilden, Germany) including DNase digestion. The samples were prepared via SENSE library kit (Lexogen).