title: Amplicon Sequencing of wild type Magnesium chelatase Subunit I gene from Sugarcane
design: For Next Generation Sequencing amplicons of 574bp were generated then purified.  Complete amplicon sequencing was performed by the CCIB DNA Core Facility at Massachusetts General Hospital (Cambridge, MA). Adapters with unique barcodes were ligated onto each sample during library construction. Libraries were pooled in equimolar concentrations for multiplexed sequencing on the Illumina MiSeq platform (Illumina, San Diego, CA) with 2x150 run parameters.