title: GSM6282801: Kasumi1, shNC, 2, CARM1; Homo sapiens; ChIP-Seq
library_construction_protocol: For AE KD H3K27ac ChIP-seq, cells were collected and treated with Mnase for chromatin fragmentation. For other ChIP-seq data, cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated. Then chromatin was inbubated with antibody/beads, and the DNA/protein complexes were then eluted and reverse crosslinked at 65 °C for 6 h. DNA was purified using phenol/chloroform extraction and ethanol precipitation. ChIP-seq libraries were prepared using End-It DNA End-Repair Kit, Klenow Fragment (3´→ 5´ exo-) and Quick Ligation kit. Libraries were amplified using 16 cycles on the thermocycler. Post amplification libraries were size selected using AMPure XP beads.