title: GSM5311522: STB_008; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were thawed on ice, and 200µl Chloroform added per 1ml of sample.  Samples were shaken for 15 seconds, incubated for 3 minutes at room temperature, then centrifuged at 12000g for 15 minutes at 4OC.   The upper aqueous layer was extracted.  Then manufacturer's instructions were followed (RNeasy® Midi Kit, QIAGEN), adding 70% ethanol, shaking, and then samples added to RNeasy Midi columns, and washed with Buffer RW1 and Buffer RPE.  Total RNA was eluted with H20 and stored at -80°C. Ion PITM Hi-QTM Chef Kit on Ion ChefTM instrument was used for library construction, assembling two chips from eight diluted samples at a time.  The Ion ProtonTM System was used as per manufacturer's instructions to sequence RNA.  Total RNA of 15ng per sample was used to produce a mean average of 18 million single end ~100 base pair reads per sample