title: GSM5420720: S4: MIX1444 day 1 (codon optimized); Bacillus subtilis subsp. subtilis str. 168; RNA-Seq
library_construction_protocol: Samples were harvested by mixing 5 ml cell culture with 5 ml 100% ethanol, immediately storing on dry ice before transferring to -80°C. RNA was isolated using phenol-chloroform extraction. Bacterial RNA library preparation was prepared from rRNA-depleted total RNA as described in https://doi.org/10.1002/cpnc.55 Single-end sequencing on an Illumina NextSeq 500. The read lengths were 76 bp.