title: GSM5914872: Patient AC439 replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Whole cell extracts were prepared from harvested cells and total RNA was extracted using TriReagent. Libraries are prepared with NEBNextUltra II mRNA-Seq Library Prep Kit for Illumina protocol according supplier recommendations. The purification of polyA+ containing mRNA molecules was done using poly-T oligo attached magnetic beads from 100ng total RNA