title: GSM5107457: T24_si_4; Homo sapiens; RNA-Seq
library_construction_protocol: The cell samples were thawed and RNA purification was performed using TRIzol with phasemaker tubes according to manufacturer's instructions. RNA cleanup was performed using RNeasy minelute kit (QIAGEN) and the RNA concentration was determined using Qubit with the RNA HS kit. RNA quantification and quality assessment were performed using Nanodrop 1000 (Mason Technology) and Bioanalyzer 2100 (Agilent). cDNA libraries were prepared from the RNA samples using TruSeq Stranded mRNA Library Prep Kit on the NeoPrep instrument (Illumina) according to manufacturer's instructions, and sequenced (single end 1x75 bp) using the NextSeq 500 platform (Illumina).