title: GSM5105691: S232_20201012171158_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cell pellets of growing spheres were lysed in Triazol and RNA was extracted with RNeasy Micro Kit (Qiagen); FFPE tissue slices were  extracted with Maxwell® RSC RNA FFPE Kit (promega) Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit  (Lexogen, Vienna, Austria) according to manufacturer's instructions The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the Illumina NovaSeq 6000 with a read length of 100 bp