title: GSM5104064: smIs89 embryo (SPLiT-seq); Caenorhabditis elegans; RNA-Seq
library_construction_protocol: The RNA extraction follows SPLiT-seq protocol The RNA-seq libraries were generated as previously described (Rosenberg et al., 2018) with minor modifications. After cell fixation, all the centrifugations were performed at 3220 g for 3 min according to the C. elegans cell size. The RT-primers are poly-dT and random hexamer mixture with UMIs (Unique Molecular Identifiers). All the barcodes used in this study were described the published protocol (Rosenberg et al., 2018). The first 24 barcodes were used in the first round of barcoding, named “Round1_01” to “Round1_24”, and 48 barcodes were used in the third round of barcoding, named “Round3_49” to “Round3_96”. Each cell barcode came from three rounds of barcoding. TruePrep DNA Library Prep Kit V2 constructed the sequencing library for Illumina (Vazyme Biotech #TD502) according to the manufacturer's instructions.