title: Iso-Seq (SMRT cell 1)
design: An optimized protocol based on the PacBio SMRTbell library was used to construct the PacBio SMRTbell full-length transcriptome library. Briefly, the amplified 10x full-length barcoded- cDNA was directly subjected to DNA damage repair, end repair, SMRTbell adapter ligation, and DNA polymerase binding using SMRTbell Express Template Prep Kit 2.0 (100-938-900). The libraries were sequenced on a PacBio Sequel II platform in CCS mode.