title: GSM6281905: H460 T2 rep1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA (gDNA) was isolated using the Blood & Cell Culture DNA Midi (5e6–3e07 cells) (Qiagen, cat. no. 13343), or Mini (<5e6 cells) Kits (Qiagen, cat. no. 13323) as per the manufacturer's instructions. For PCR amplification, gDNA was divided into 100 μl reactions such that each well had at most 4 μg of gDNA. Each well consisted of 66.5 μl gDNA plus water, 23.5 μl PCR master mix (20 μl Buffer 5X, 2 μl dNTPs 10 μM, 1.5 μl GoTaq; Promega, cat. no. M3001), and 5 μl of Forward universal primer, and 5 μl of a uniquely barcoded P7 primer (both stock at 10 μM concentration). PCR cycling conditions: an initial 2 min at 95 °C; followed by 30 s at 95 °C, 40 s at 60 °C, 1 min at 72 °C, for 22 cycles; and a final 5 min extension at 72 °C.