title: GSM5792020: MI-E; Mus musculus; RNA-Seq
library_construction_protocol: For 10X Genomics scRNA-seq, cells were isolated from femur and tibia of Sham, MI and MI-E, which were further purified via density gradient centrifugation using Ficoll-PaqueTM media. The barcoded libraries were prepared under Single Cell 3' Reagents Kit v3.1 (10X Genomics) according to the manufacturer's protocol. Single cell suspensions were loaded on Chromium Controller (10X Genomics) to form single cell gel beads in emulsion (GEMs). The libraries were further controlled via Agilent 2100 Bioanalyzer (Agilent Technologies). After passing quality control, the libraries were sequenced on NovaSeq 6000 (Illumina) to generate 150 bp paired-end reads. The barcoded libraries were prepared under Single Cell 3' Reagents Kit v3.1 (10X Genomics) according to the manufacturer's protocol.