title: GSM5231113: S266_B cell; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were partitioned into nanoliter-scale Gel Bead-In-EMulsions (GEMs) to achieve single cell resolution for a maximum of 10,000 individual cells per sample. Utilizing the Chromium Single Cell A Chip Kit(10x Genomics, PN-120236) and Chromium Single Cell 5' Library & Gel Bead Kit (10x Genomics, PN-1000006) , poly-adenylated mRNA from an individual cell was tagged with a unique 16 base pair 10x barcode and 10 base pair Unique Molecular Identifier. Libraries were generated using the Chromium Single Cell 5' Feature Barcode Library Kit  (10x genomics, 1000080) and the Chromium Single Cell V(D)J Enrichment Kit Human B Cell (10x Genomics, PN-1000016). The cDNA amplicon size for each library was optimized using enzymatic fragmentation and size selection and the concentration and average fragment size of each library was determined using an Agilent High Sensitivity DNA Kit (Agilent). Specifically, user guide CG000186 Rev D was used for all steps. All libraries were sequenced on an Illuina NextSeq550 or an Illumina NextSeq500 with 26 cycles apportioned for read 1, 8 cycles for the i7 index, and 134 cycles for read 2 using an NextSeq 500/550 High Output Kit v2.5 (150 Cycles). Some samples were sequenced using a NextSeq 550 housed within the Pritzker School of Molecular Engineering's Chicago Immunoengineering Innovation Center (Chicago, IL) and others on a NextSeq500 belonging to the University of Chicago Genomics Facility (Chicago, IL - RRID: SCR_019196)