title: GSM5007865: EG1 p300i Rep B input; Mus musculus; ChIP-Seq
library_construction_protocol: 5 million cells were cross-linked with 1% formaldehyde, quenched, and snap frozen until ready to proceed. 5 million formaldehyde-fixed Drosophila nuclei were added to each sample prior to sonication for normalization. After fragmentation, inputs were set aside, and remaining chromatin was immunoprecipitated using H3K27ac antibody and stringent washes. After elution, DNA was reverse-crosslinked and then purified using ZYMO kit. 25ng of immunoprecipitated (ChIP) or input (input) material were used for library prep. Libraries were preared using KAPA HyperPrep kit according to manufacturer's instructions.