title: GSM5106560: 2508-24h; Mus musculus; RNA-Seq
library_construction_protocol: Cells were harvested after culture and RNA was isolated using Macherey&Nagel RNA isolation kit according to the manufacturers protocol. Samples concentration was measured with Qubit Fluorometer (ThermoFischer). RNA integrity was assessed using Agilent 2100 BioAnalyser. RIN values were between 7.7 and 9.4. mRNA-Seq libraries were prepared from 100ng of input total RNA samples using TruSeq stranded mRNA kit (Illumina). Barcoded and pooled libraries were sequenced on 5,5 lanes of Illumina HiSeq 4000 platform (HCS version 3.3.76, RTA version 2.7.6) in a paired-end 2x75 nt sequencing mode.