title: GSM5231127: Testes_KO_TAM13_FP1_S37; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from mouse testicles was isolated using the Direct-zol RNA Miniprep Kit (Zymo Research, Irvine, CA) following manufacturer's instructions. RNA concentration was measured using the NanoDrop™ OneC (Thermo Scientific, Waltham, Massachusetts). The RNA Integrity Number (RIN) was assessed using the 4200 TapeStation system and the TapeStation RNA ScreenTape & Reagents (Agilent Technologies, Santa Clara, CA). RNA sequencing libraries were generated using the Illumina TruSeq Stranded Total RNA Library Prep Gold with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer's instructions, except modifying RNA shear time to five minutes. Resulting libraries were multiplexed and sequenced with 100 basepair (bp) Paired End (PE100) to a depth of approximately 25-40 million reads per sample on an Illumina NovaSeq 6000. Samples were demultiplexed using bcl2fastq v2.20 Conversion Software (Illumina, San Diego, CA).