title: ddRad-seq of A1-Nisa: young leaf rep1
design: Genomic DNA was doubly digested with HindIII and Sau3AI. The fragments were ligated with primer adapters onto the restriction enzyme cut sites, purified with AMPure XP beads, were enriched using PCR while attaching the i5 and i7 index primers (Nextra XT Index Kit v2 Set A and Set B), and again purified using AMPure XP beads. Fragments between 295 and 614bp were then selected using SageELF. A final AMPure XP bead purification was done on the selected fragments. Equimolar amounts of all samples were pooled and sequenced using a single lane of HiSeq XTen.