title: GSM6283393: INTS11-AID cells, input, rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: 15cm plate cells were fixed with 1% formaldehyde for 10 min. The glycine crosslinking at 0.125M was terminated for 10min, the lysates were incubated on ice for 20min, and the chromatin was ultrasonically interrupted by ME220 focused ultrasound (Covaris) The chip-seq library was prepared using the general DNA library construction kit (Vazyme, NDM607-02), and 100ng of DNA was taken as the starting material of the input sample and IP sample. The library was cyclically amplified 13 times on the thermal circulator. The DNA magnetic beads were recovered with 2% agarose gel, and the amplified library sizes of 250-400bp were selected. Library concentrations were measured using the Qubit™ ssDNA Assay Kit (Invitrogen, Q10212). Q-seq detects library fragment size