title: Metagenome of Halichondria sitiens: bacterial fraction
design: DNA was extracted from bacterial fractions obtained from 1 cm3 fragment of sponge tissue using the Diatom DNA Prep kit (Galart Diagnosticum). DNA was extracted twice with phenol-chloroform and chloroform and precipitated in ethanol for the additional purification. NGS libraries were made using NEBNext Ultra II DNA Library Prep kit (NEB).