title: GSM5105558: 18465X46 14d RL Leucine 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated by homogenizing 25-30 mg gastrocnemius tissue with a handheld homogenizer in a solution containing Qiazol Lysis Reagent (Qiagen, cat# 79306). The RNA was separated and precipitated using chloroform and isopropanol. Extracted RNA was washed with ethanol then suspended in nuclease-free water. RNA concentration was determined using the EPOCH (Take3, BioTek) spectrophotometer. RNA isolated from gastrocnemius underwent DNase digestion using TURBOTM DNase (ThermoFisher, cat# AM2238) and was purified using RNA Clean and Concentrator 5 Columns (Zymo Research, cat# R1014). Libraries were prepared with Illumina Stranded Total RNA Library Prep Ribo-Zero Gold Plus (Genome Builds mm10, M_musculus_Dec_2011, GRCm38) and RNA was sequenced using Illumina NovaSeq S4 Reagent Kit v1.5 150x150 bp Sequencing