title: GSM5104938: wt 1; Danio rerio; RNA-Seq
library_construction_protocol: Trizol homogenization followed by column-based RNA purification (Zymo Direct-zol Kit). For RNAseq, RNA integrity and concentration were checked on a Fragment Analyzer (Advanced Analytical) and libraries were prepared using the Illumina NeoPrep RNAseq kit. Blue Pippin size selection was performed, selecting for 350 nt fragments. Libraries were quantified using the Fragment Analyzer (Advanced Analytical) and qPCR before being loaded for paired-end sequencing 2x75 nucleotides using the Illumina NextSeq 500 in High Output mode. polyA-based mRNA capture using Neoprep with TruSeq strand-specific strategy.