title: GSM5172440: met2cd_eemb_20°C_rep2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Extraction of RNA was performed according to the WormBook protocol (Stiernagle, 2006). Total RNA was purified using Trizol and further purified using the RNA Clean & Concentrator kit (Zymo Research R1015) including DNase treatment. Depletion of ribosomal RNA was done for 5 ľg of total RNA with Ribo-Zero Margnetic Gold Kit (Epicentre MRGZG12324) and further concentrated with RNA Clean & Concentrator kit (Zymo Research R1015) according to corresponding manufacturers instructions. Libraries were prepared using the TruSeq Total RNA-seq (stranded-high input) Library Prep Kit (Illumina, San Diego, CA, USA).