title: GSM5467045: screen-CkoC44-T15-25uM-R1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted for T0, T6 and T15 of each triplicate of each condition using the Wizard Genomic DNA Purification kit (Promega, A1125) following manufacturer's instructions. Sequencing libraries were prepared by amplifying gRNA inserts via a 2-step PCR. The first PCR enriches for the gRNA regions in the genome and the second PCR adds indices by using primers that include Illumina TruSeq adaptors. The libraries were sequence on Illumina HiSeq2500 single-end reads