title: DNA-Seq of G.oxydans with plasmid pBBR1MCS-5-rhaSR-PrhaBAD-mNG
design: Genomic DNA was purified using a Microbial DNA Mini kit (MACHEREY-NAGEL, Dueren, Germany) from an overnight culture. DNA concentration was measured using the Qubit 2.0 fluorometer (Thermo Fisher Scientific, Waltham, USA). Afterwards, 1 g of DNA was used for library preparation using the NEB-Next Ultra II DNA Library Prep Kit for Illumina (NEB, Frankfurt am Main, Germany). Library evaluation was done by qPCR using the KAPA library quantification kit (Peqlab, Erlangen, Germany). Afterwards, normalization for pooling was done and paired-end sequencing was performed using a Miseq (Illumina, San Diego, CA), with a read length of 2  150 bases. Sequencing output (base calls) were stored as demultiplexed fastq files.