title: Ms50
design: Encapsidated viral nucleic acids were enriched from grounded material by 0.45um filtration, ultracentrifugation and by DNase and RNase treatment. DNA and RNA nucleic acids were extracted, reverse transcription was performed by SuperScript III reverse transcriptase. After cDNA purification, second strand DNA was synthesised using Klenow fragment DNA polymerase. Double-stranded DNA was amplified by random PCR amplification. Samples were barcoded during reverse transcription and PCR steps using homemade 26-nt Dodeca Linkers and PCR multiplex identifier primers. PCR products were purified and libraries were prepared from purified amplicons and sequenced on an Illumina MiSeq V3 chemistry. Adaptors were removed and reads were filtered for quality (q30 quality and read length >45nt).