title: GSM5421403: Astrocyte Npas3-/- rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from astrocytes (DIV 14) by TRIzol method. Then, total RNA was quantiﬁed and quality controlled by Agilent Bioanalyzer 2100 system . And 500ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.