title: GSM5652638: Lyz2-KLF2-KO_rep1 [CWRU006087_S4]; Mus musculus; RNA-Seq
library_construction_protocol: Mouse hearts were harvested and digested by collagenase to single cell suspension. Neutrophils were sorted by flow cytometry. Dead cells were excluded by live-dead dye staining. RNA was extratced from neutrophils by a Qiagen RNeasy micro kit and subjected to RNA-seq using paired-end sequencing. RNA libraries were prepared for sequencing using the SMART-Seq v4 for CDNA and the Nextera XT kit for library creation