title: GSM5106389: s06_hda3_biorep2; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Cell grindate (1 gram) was dissolved in cold Lysis buffer. Chromatin was solubilized by DNase I treatment. Nascent RNA fraction was enriched by immunoprecipitation of FLAG-tagged RNAPII elongation complexes using anti-FLAG antibody (F3165, Sigma-Aldrich) coupled to Dynabeads Protein G. Finally, RNAPII complexes were eluted from Dynabeads by 3xFLAG peptide, and the nascent RNA was purified using miRNeasy kit (QIAgen). NGS libraries were constructed from nascent RNA using NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific) following the protocol described in Kindgren et al., 2020 (PMID 31863587).