title: GSM5106978: CD105_rep2; Mus musculus; ATAC-seq
library_construction_protocol: 3 month old PolG mouse femurs were isolated, subjected to mechanical and enzymatic digestion, different skeletal cell subsets were then isolated by FACS. Cells were spun down, lysed and tagmented using Tn5 enzyme (Illumina Tag DNA Enzyme & Buffer Small Kit, 20034197). The tagmented DNA samples were purified using the MinElute PCR kit (Qiagen, cat no 28004) and eluted with 20 μl of ddH2O. ATAC-Seq libraries were prepared and samples were sequenced on an Illumina NovaSeq6000 sequencer with paired-end 100 bps.