title: GSM5700399: B6_Lmajor_Gp63_rescue-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the aqueous phase from the Trizol lysates using EasyPure RNA columns as per the manufacturer's protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). Ribosomal RNAs were depleted and libraries were prepared with the Kapa Hyperprep stranded total RNA kit (Roche). The indexed libraries were pooled and the pool was sequences on 2 lanes of Illumina NovaSeq 6000 in a paired-end 50bp configuration.