title: GSM5420961: WT_GSK862_methyl_rep1; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Cells were harvested, and then genomic DNA was extracted via Qiagen Puregene Cell Kit (catalog# ) 500 ng of genomic DNA were sheared using a LE220-plus Focused-ultrasonicator. Sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) without PCR amplification. Post-ligation cleanup proceeded according to Illumina's instructions with 110 µL Sample Purification Mix from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina catalog # FC-151-1002). After purification, 4 samples were pooled equivolume and methylome regions were captured using EPIC oligos. Capture pools were bisulfite converted and amplified with 11 cycles of PCR. Pools were sequenced on a HiSeq 2500 in Rapid mode in a 100bp/100bp paired end run, using HiSeq Rapid SBS Kit v2 (Illumina). The average number of read pairs per sample was 61 million.