title: GSM5420994: Heart CTL rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from homogenyzed tissues using the TRIzol reagent (Invitrogen) according to  manufacturer's instructions After Ribogreen quantification and quality control, 500 ng of total RNA with RIN values of 7.0-10 underwent polyA selection and TruSeq library preparation according to instructions  provided by Illumina (TruSeq Stranded mRNA LT Kit). An Average of 34 million paired reads was generated per samples. Libraries generated from the colon were run on an Illumina HiSeq  2500 machine and generated using the HiSeq SBS Kit v4 generating single reads 50 bp long.