title: RNA-Seq of Brassica napus mediator subunit16 overexpression line
design: The samples (three biological replications) were subjected to RNA extraction, mRNA library construction, and RNA sequencing (MGISEQ2000 sequencing platform, BGI Co., Ltd, Shenzhen, China). To identify genes corresponding to reads from each sample library, the reads were aligned to the B. napus reference genome (http://brassicadb.org/brad/datasets/pub/BrassicaceaeGenome/Brassica_napus/Brassica_napus.annotation_v5.gff3.gz) and the S.  sclerotiorum genome (http://www.broadinstitute.org/annotation/genome/sclerotinia_sclerotiorum/MultiHome.html) using TopHat. Gene expression levels were estimated based on fragments per kilobase of exon per million fragments mapped (FPKM), which were used to draw heatmaps for the differentially expressed genes (DEGs) identified with DEGseq based on the criteria q-value <0.005 and |log2 (FPKM-transgenic/FPKM-WT)| >1 between transgenic and WT lines. For gene ontology (GO) term annotations, all B. napus genes were searched against the National Center for Biotechnology Information (NCBI) non-redundant (Nr) protein database using GOSeq with corrected P-value <0.05.