title: GSM5419913: Articular chondrocytes transfected with Sephs1 siRNA, rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRI reagent (Molecular Research Center, Inc.). One microgram of total RNA was converted into cDNA libraries using the TruSeq Stranded mRNA Sample Prep kit (Illumina). Final cDNA libraries were analyzed for size distribution, quantified by qRT-PCR using Agilent 2100 Bioanalyzer, and normalized to 2 nM for sequencing.