title: GSM5231224: Knockout sample C; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in TRIzol for RNA extraction. The libraries were prepared from 100-200 ng total RNA enriched for mRNA using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina (New England Biolabs, USA) and sequenced on an Illumina NextSeq 2000 instrument at 650 pM (Illumina, USA).