title: GSM5425206: HD_2 - BulkRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Micro Kit (Qiagen, 74004) and RNA quality was analyzed using a Bioanalyzer 2100 (Agilent) using PICO chips. RNA-Seq libraries were prepared using the TruSeq RNA sample prep v2 kit (Illumina TruSeq Stranded mRNA, RS-122-2001) and 100 ng total RNA following the manufacturer's instructions. The amplified libraries were purified using AMPure beads (Beckman Coulter, A63881), quantified by Qubit 2.0 fluorometer (Life Technologies), and visualized using an Agilent Tapestation 2200. The libraries were pooled equimolarly, loaded on the HiSeq 2500 DNA Sequencer (Illumina) and run as single 50 nucleotide reads.