title: 16S of P.manillensis-01
design: Raw DNA was amplified using TransGen AP221-02: TransStart Fastpfu DNA Polymerase (TransStart, Beijing, China) on ABI GeneAmp 9700 (Thermo Fisher Scientific, US). Amplifications were performed with three replicates. The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturers instructions and quantified using Quantus Fluorometer (Promega, USA). The sequencing library was prepared following previous study