title: GSM6283287: MB_ctrl_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from myoblasts and myotubes incubated with various overnight conditions was isolated using TRIzol extraction. Purification of extracted RNA was performed using Zymo RNA Clean and Concentrator Kit with DNase (Zymo Research, Irvine, CA, cat. #R1018) according to the manufacturers' instructions. Quantification of total RNA was performed using a nanodrop system. 5 ng of RNA was used as an input for the Smart-seq2 protocol as described in Picelli et al. (61,62) including cDNA synthesis, pre-amplification, tagmentation, and enrichment steps. Smart-seq2