title: GSM5652633: Tumor endothelial cells (TECs); Mus musculus; RNA-Seq
library_construction_protocol: Tumors or normal tissues from control mice or Dnmt1iECKO mice were minced into small pieces and incubated for 90 mins. at 37 °C in dissociation buffer containing 2 mg/mL of collagenase type I (Worthington), one mg/mL of DNase (Worthington), and 2.5 units/mL of neural protease (Worthington). The digested tissues were separated into a single-cell suspension by passing through a 100 mM cell strainer. Single cell isolates were stained with primary antibodies for 30 mins. on ice, followed by secondary antibodies, and then washed using FACS buffer. Samples were fixed with 2 % paraformaldehyde and then analyzed by flow cytometry on a FACSCaliber (BD) or Accuri C6. These isolated ECs were then subjected to scRNA sequencing.