title: Illumina MiSeq sequencing; Marker Frequency data of genomic loci extracted  from 7 engineered N16961 V. cholerae strains (M9 samples were done in duplicates)
design: Marker Frequency data of genomic loci extracted  from 7 engineered N16961 V. cholerae strains (M9 samples were done in duplicates)
library_construction_protocol: Bacterial strains were cultivated at 30C either in M9 Minimal medium or LB rich medium either to exponential phase (0.2 OD 650nm) or to stationnary phase (overnight culture). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol.   The libraries and the sequencing were performed by the High-throughput Sequencing facility of the I2BC (http://www.i2bc.paris-saclay.fr/spip.php?article399〈=en,CNRS, Gif-sur-Yvette, France). Genomic DNA libraries were made with the 'Nextera DNA library preparation kit' (Illumina) following the manufacturer's recommendations.