title: HiSeq X Ten paired end sequencing; Single cell RNA-seq of the Dppa3-KO mouse embryos at 4-cell stage and 8-cell stage
design: Single cell RNA-seq of the Dppa3-KO mouse embryos at 4-cell stage and 8-cell stage
library_construction_protocol: Stella−/− mice have been described previously (Nakamura T, Arai Y, Umehara H, Masuhara M, Kimura T, Taniguchi H, Sekimoto T, Ikawa M, Yoneda Y, Okabe M, et al: PGC7/Stella protects against DNA demethylation in early embryogenesis. Nat Cell Biol 2007, 9:64-71.). RNA was isolated from each single cell of two 8-cell stage Stella−/− embryos with the Smart-seq2 protocol.  To generate RNA sequencing libraries, a KAPA Hyper Prep Kit was used following the manufacturer's instructions. The adapters used were from a KAPA Single-Indexed Adapter Kit.