title: GSM5421860: P0 Npas3+/- rep1; Mus musculus; RNA-Seq
library_construction_protocol: Cortices were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Then, total RNA was quantiﬁed and quality controlled by Agilent Bioanalyzer 2100 system. And 500ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.