title: GSM5007893: Mit DMSO Rep A; Mus musculus; Hi-C
library_construction_protocol: 2 million cells were cross-linked with 1% formaldehyde, quenched, and snap frozen until ready to proceed. Arima Hi-C kit was then used to digest, fill/mark ends with biotin, and ligate DNA. Arima Hi-C kit along with KAPA Hyper Prep reagents were used for library construction. Ligated DNA was fragmented using Pico bioruptor and size selected, then biotin enrichment was performed. After End repair and dA-tailing, adapter ligation was performed using SeqCap Adapter Kit A & B indexes.