title: GSM5700170: CLPs, Notch1+/- (PAJ011719-006); Mus musculus; RNA-Seq
library_construction_protocol: 4-11x10^6 adult BM CLP cells from WT, Notch1+/-, or Notch1+/ΔTAD were sorted into RNA lysis buffer (Invitrogen) and stored at -80ºC until RNA extraction. Total RNA was extracted using PicoPure™ RNA Isolation Kit (ThemoFisher Scientific, USA) with inclusion of DNase treatment step. RNA sequencing libraries for Illumina sequencing were prepared in one batch using strand-specific QuantSeq 3' RNAseq kit (Lexogen, FWD, Cat. No. 015) along with negative and positive controls.