title: Next-generation WGS of Streptococcus pneumoniae AMR38
design: The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed and further ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.