title: GSM5420715: XT2; Gallus gallus; RNA-Seq
library_construction_protocol: Three pectoralis major tissue samples from the control group and three from vitamin E supplement group were used for RNA extraction and sequencing. Total RNA was isolated from the samples using the RNAsimple Total RNA kit (Tiangen Biotech Inc., Beijing, China) in accordance with the manufacturer's instructions. Integrity and concentration of the RNA were evaluated using an Agilent 2100 Bio-analyzer (Agilent Technologies, Palo Alto, CA, USA) to meet subsequent quality requirements for sequencing. The mRNA was enriched from total RNA samples using oligo (DT) magnetic beads (Invitrogen, USA) after them treated with Ambion DNase I (Life Technologies Inc.). First-strand cDNA was synthesized after purified mRNA fragmented by RNA fragmentation kit (Ambion, USA), then the second-strand cDNA were synthesized according to the manufacturer's instructions. Quality of the cDNA library was assessed using Agilent 2100 Bioanalyzer and ABI Step One Plus Real-Time PCR System. Finally, the qualified cDNA libraries were sequenced using the Illumina HiSeq 2500 system.