title: DNA sequencing of mouse gut metagenome
design: DNA was extracted from stool samples using a mechanical bead-beating approach with the Mini-Beadbeater-16 and 1-mm diameter zirconia/silica beads followed by the Qiagen QIAamp DNA Stool Mini Kit. Bead-beating consisted of 7 rounds of alternating 30 s bead-beating bursts followed by 30 s of cooling on ice. Short-read sequencing libraries were prepared with the Illumina Nextera XT kit. Libraries were sequenced on an Illumina HiSeq 4000.