title: DNA barcode-Seq of zooxanthellae
design: All DNA was used as the templates for amplicon generation of zooxanthellae ITS2. Amplicons were quantified and qualified to establish libraries using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) following manufacturer's recommendations, and index codes were added during the preparation. Library was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system, and sequenced on an Illumina NovaSeq platform using 250 bp paired-end reads (Novogene, Beijing).