title: GSM5050862: PERK_KO_M2_1A; Mus musculus; RNA-Seq
library_construction_protocol: Firstly, the biotin-labeled Ribo-Zero rRNA Removal Kit is used to remove ribosome rRNA of total RNA.Then the un-purified RNA is fragmented under a certain temperature and ion environment. Random primers and reverse transcriptase of TruSeq® Stranded kit are used to synthesize the first-strand cDNA. cDNA library sequencing was performed using Illumina HiSeq RNA libraries were prepared for sequencing using standard Illumina protocols