title: RNA-Seq of S. pombe
design: The single-chromosome 2c-1-3ci cells from log-phase cultures (OD600 = 1) were collected, and their RNA was isolated using the TRIzol (Invitrogen) method. The library preparation followed the standard procedure (Illumina). The libraries were sequenced on the Illumina NovaSeq 6000 platform using the 150-bp paired-end sequencing strategy. For each sample, 6 Gb of clean data was obtained.