title: GSM5701150: IgAKO2; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow, small intestine lamina propria and spleens were harvested at time of saccrifice and lypmhocytes were stained and FACS sorted twice directly into TRIZOL reagent as follows: bone marrow plasma cell: Dump[CD4, CD8a, F4/80, Ter119]-, IgD-, GFP(Blimp1)+, CD138+, IgA+ or IgM+ or IgA/M-; siLP plasma cell Dump[CD4, CD8a, F4/80, Ter119]-, IgD-, GFP(Blimp1)+, IgA+;   follicular B cell: live, singlet, CD19+, CD93-, CD23+, CD21/35int.  marginal zone B cell: live, singlet, CD19+, CD93-, CD23-, CD21/35high. RNA was extracted by TRIZOL protocol and quantified by Qubit.  cDNA was prepared using Takara Clonetech SMART-Seq HT kit by protocol with Illumina Nextera XT reagents for library construction and quantified and equal molar pooled by tape station and qubit analysis.