title: GSM5106726: SARS-CoV-2-infected sample at time = 8 hours; replicate 3; Homo sapiens; ChIP-Seq
library_construction_protocol: 30 sec OFF cycles twice using the Bioruptor Pico. Each sonicated lysate was then pre-cleared using 10ul of Rabbit-IgG Dynabeads for 1 hour, rotating at 4oC. 1ug of anti-H3K27ac antibody was then added to 300ul of pre-cleared lysate. Immunoprecipitation (IP) was performed with overnight rotation at 4oC. To recover IP-complexes, 10ul of Dynabeads M-280 Sheep anti-Rabbit IgG were added to each reaction and tubes were rotated for 2 hours at 4oC. Bead-chromatin complexes were then washed 6 times on a magnet using ice cold RIPA wash buffer [50mM Hepes-KOH pH7.6, 100mM LiCl, 1mM EDTA, 1% NP-40, 0.5% Na-Deoxycholate]. Washed beads were then incubated in 125ul Elution buffer [1% SDS, 0.1M NaHCO3] at 65oC overnight for elution and de-crosslinking. ChIP DNA was then purified using the MinElute PCR purification kit (Qiagen) and quantified using the Qubit 1X dsDNA HS Assay Kit. 550 High Output Kit v2.5.