title: HiSeq whole genome sequencing of Ralstonia solanacearum QL-Rs1115: samples exposed to high concentrations of volatile organic compounds
design: H-VOC treatment QL-Rs1115 bacterial strain population replicates were grown in CPG media (30C and 170 rpm) with the addition of a blend of high concentration volatile organic compounds (200 ppm). After two-day, 25 l of liquid culture from these flasks was inoculated into fresh flasks containing 25 ml of CPG medium and after adding the high concentration blend of volatile organic compounds, these flasks were also incubated at 30C and 170 rpm for two days. This process of inoculation and re-inoculation continued for 22 days. gDNA was extracted using DNeasy Kit (QIAGEN) according to the manufacturers instructions.