title: GSM5424930: MG824DMSOCTL; Mus musculus; RNA-Seq
library_construction_protocol: Tri Reagent was added to the culture plate, and the plates were shaken for 5 minutes. RNA was isolated according to manufacturer's instructions. 5ng of total RNA was reverse transcribed with SuperScript II (Thermo Fisher) using an oligo-dT RT primer with a PCR binding site and a template switching oligonucleotide with a homotypic PCR binding site. Betaine and MgCl2 were added to enhance the reaction. After reverse transcription, whole transcriptome amplification by PCR was performed for 12 cycles using HiFi HotStart ReadyMix (KAPA) with a PCR primer. The PCR reaction was cleaned up with Ampure XP beads. The amplified DNA was diluted to a concentration of 0.5ng/ul, and subjected to tagmentation with the Illumina Nextera XT kit. Each sample was PCR amplified with a unique set of Nextera indices.