title: RNA-Seq of Syntrichia caninervis: site 14: UV-transmitted
design: Approximately 20 mg of dry weight per sample were placed into microcentrifuge tubes and sent to Novogene (Sacramento, CA, USA) for RNA extraction, library preparation, and transcriptome sequencing. Samples were processed according to the standard Novogene protocol, including preliminary quality check gel electrophoresis, quantitation, and purity assessment with NanoDrop (ThermoFisher Scientific, Waltham, MA, USA), and sample integrity assays with a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). After quality checking procedures, oligo(dT) beads were used to enrich eukaryotic mRNA, and rRNA was removed with the Illumina Ribo-Zero kit (Illumina, Inc., San Diego, CA, USA). RNA samples were then reversetranscribed into double-stranded cDNA libraries and sequenced on the 150 bp paired-end Illumina NovaSeq 6000 platform.