title: GSM5792451: 2 days_CIA_mouse77; Mus musculus; RNA-Seq
library_construction_protocol: For mouse paws, frozen tissues were homogenized in 1.2 ml of QIAzol Lysis Reagent (Qiagen) per sample using a TissueLyser (Qiagen) for 2 x 5 min at 30 Hz, 4°C. The lysates were centrifuged for 5 min at 12,000 g, 4°C to remove debris. 250 µl of supernatant was used for RNA isolation using the Direct-zol-96 RNA kit (Zymo Research) by following the manufacturer's instructions. For mouse paws, 1 µg of total RNA was used as an input material for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). The libraries were multiplexed and paired-end sequenced (2 × 75 bp) on NextSeq 550 instrument (Illumina) using the NextSeq 500/550 High Output Kit (150 cycles).