title: GSM5554362: trZMYND11K36_S10; Homo sapiens; OTHER
library_construction_protocol: For CUT&RUN, cells were bound to activated ConA magnetic beads, permeabilized and incubated with antibody, overnight at 4C. After washing, cells were incubated with pAG Mnase. DNA extract was purified using the NEB monarch DNA cleanup kit. Finally, 5–10 ng of purified CUT&RUN-enriched DNA was used to prepare Illumina library using the NEB Ultra II DNA Library Prep Kit per manufacturer's instructions.