title: GSM5101058: 200_ng_B1_IL; Homo sapiens; RNA-Seq
library_construction_protocol: 1. Swift RNA Library Prep. 100, 50 and 10 ng of UHRR was used as direct input for NEBNext Ploy(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490). In the final elution step, mRNA was eluted off the beads into the Swift RNA Library Kit Fragmentation Buffer. Libraries were prepared and indexed (Swift Unique Dual Indexing Kit, X9096) using Swift recommended protocol. cDNA libraries were diluted 1:5 in low TE buffer and analyzed on Agilent 4200 TapeStation using D5000 HS DNA screentape. Libraries that gave yields < 4 nM were amplified using the Swift Library Amplification Primer Kit (88196). 2. Swift Rapid RNA Library Prep. 200, 100 and 50 ng of UHRR was used as direct input for NEBNext Ploy(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490). In the final elution step, mRNA was eluted off the beads into the Swift RNA Library Kit Fragmentation Buffer. Libraries were prepared and indexed (Swift Unique Dual Indexing Kit, X9096) using Swift recommended protocol. cDNA libraries were diluted 1:1 in low TE buffer and analyzed on Agilent 4200 TapeStation using D5000 DNA screentape. 3. Illumina TruSeq Stranded mRNA Library Prep. 500, 200, 100 and 50 ng of UHRR was used as direct input for the Illumina TruSeq Stranded mRNA (20020594). Libraries were prepared and indexed (IDT for Illumina-TruSeq RNA UD Indexes, 20022371) as outlined in the TruSeq Stranded mRNA Reference Guide.