title: GSM5652645: 23-WT-Con-RNA-4; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed and collected from 35 mm plates using TRI reagent. RNA was purified by Directzol Microprep kit with Dnase digestion. RNA was extracted from wild-type and P2ry1 receptor knockout BetaTC6 β-cell lines by Tri-reagent followed by Directzol purification. 125 ng of RNA was processed, barcoded, and amplified 11 cycles using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina with polyA bead selection and then sequenced by paired-end 42x42 on an Illumina NextSeq 500