title: GSM5106123: H9 human embryonic stem cells line (WA09) passage 40s [P1-1]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using Easy-blue reagent (Intron, #17061) according to the manufacturer's instructions. To construct a sequencing library, we used a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly, the steps for the strand-specific protocol are as follows: first strand cDNA synthesis; second strand synthesis using dUTPs instead of dTTPs; end repair, A-tailing, and adaptor ligation; and PCR amplification.