title: Oxford Nanopore sequencing of Candida metapsilosis strain 414
design: C. metapsilosis 414 was cultured on YPD agar for 48 h at 30 degrees Celsius, then grown overnight in 50 ml YPD broth at 30 degrees Celsius, 200 rpm. DNA was extracted using the QIAGEN Genomic-Tip (100/G) kit as per kit instructions. DNA quality was checked with NanoDrop and quantified using the Qubit fluorometer. Libraries were prepared for minION sequencing and barcoded with the Rapid Barcoding Kit (SQK- RBK004) from Oxford Nanopore Technologies (ONT). Prepared libraries from multiple samples were pooled and loaded onto an ONT minION flow cell (FLO-MIN106) for sequencing. The sequencing run lasted 48 h, producing 7.6 gigabases (Gb) of data total for three yeast samples, including C. parapsilosis, Hanseniaspora uvarum and C. metapsilosis 414. Basecalling for the minION data was performed using the ONT Guppy software, version 3.2.4+d9ed22f with the following parameters; --input_path fast5 -- save_path fastq --flowcell FLO-MIN106 --kit SQK-RBK004 --verbose_logs -- cpu_threads_per_caller 5 --num_callers 7. Basecalled data were demultiplexed using qcat version 1.1.0 with the following parameters; --fastq fastq/all_multiplexed_reads.fastq --barcode_dir demultiplex_qcat --detect-middle -- min-read-length 1 --trim --kit RBK004 --epi2me. After demultiplexing, 2.79 Gb of data were assigned to C. metapsilosis 414