title: GSM5311806: CF702; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from primary human intestinal fibroblasts isolated from the normal and stenotic intestines of CD patients using TRIzol reagent. Purify and fragment mRNA, synthesize first strand cDNA, synthesize second strand cDNA,  purification, adenylate 3' ends, ligate adapters, purification, enrich DNA fragments and purification in turns. RNA libraries were prepared for sequencing using standard illumina protocols