title: RNA-Seq of Danio rerio larvae
design: Oligo (dT) enriched mRNA was randomly fragmented using fragmentation buffer and then cDNA first-strand was synthesised by reverse transcriptase with random hexamers. Second-strand synthesis was by Escherichia coli polymerase I nick-translation with dNTPs and RNase H. The final cDNA library was completed using terminal repair, adapter ligation, size selection, PCR enrichment and A-tailing.