title: GSM6281854: M; Mus musculus; RNA-Seq
library_construction_protocol: Mitral leaflets were dissected from E17.5 control and mutant embryos and digested with 0.05% Trypsin to obtain single cells. For each genotype, samples were pooled from 3-4 hearts. To collect viable single cells, 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences). Preparation of the single cell transcriptome libraries was performed using 10xGenomics Single Cell 3ʹ v3 Reagent Kits, following the manufacturer's instructions.