title: GSM5554067: Cells-WT-42: Wild type dendritic cells, mouse 42; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using Tri-Reagent (Sigma-Aldrich) as per the manufacturers' instructions. SEV RNA was extracted using Trizol LS (Invitrogen) then purified further using Qiagen RNA Mini kit. RNA yield was quantified using NanoDrop and RNA quality was assessed using either the TapeStation System 2200 or the Bioan-alyser (Agilent Technologies). To remove contaminating DNA, 1μg RNA was treated with 1μl (2 U) of DNAse I (Ambion) in a 20μl reaction incubated at 37 °C for 30 minutes and 1μl DNAse inactivation reagent added. RNA libraries were prepared from 200ng of total RNA using a TruSeq RNA puri-fication kit, and RNA-seq was performed on an Illumina NextSeq platform, generating 80 base pair (bp) paired-end reads.