title: NextSeq 500 sequencing; PRMT5 genomic deregulation supports growth and stress tolerance in chronic lymphocytic leukemia
design: PRMT5 genomic deregulation supports growth and stress tolerance in chronic lymphocytic leukemia
library_construction_protocol: Cell lines were taken from the stock of the cell lines bank (Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures). Cell lines were authenticated by DNA profiling and cytogenetics. Factor bound DNA was isolated via Cleavage Under Target and Release Using Nuclease, CUT and RUN. KAPA HyperPrep generated libraries, quality controlled via Applied Biosystems Fragment fluorometer.