title: GSM6281758: U937-AE_AE_48h; Homo sapiens; ChIP-Seq
library_construction_protocol: U937-AE and U937-EG were cross-linked with 1% formaldehyde for 10min and stopped by 125mM glycine. After lysis and sonication, 30μL of anti-HA magnetic beads (MCE, HY-K0201) was incubated with chromatin samples overnight at 4℃ for immunoprecipitation. The DNA were purified with the MinElute PCR Purification Kit (QIAGEN) and subjected to library construction and next-generation sequencing with Illumina systems. ChIP-seq libraries were used the VAHTS Universal DNA Library Prep Kit for Illumina V3 Kit (ND607).