title: GSM4827043: Monkey_Cerebellum_mCG; Macaca fascicularis; Bisulfite-Seq
library_construction_protocol: The methylome extraction is majorly based on post-bisulfite adaptor tagging (PBAT). DNA from tissues are extracted by phenol/chloroform/isoamylalcohol followed by isopropanol precipitation. Then extracted DNA was mixed with lambda DNA (1:200) and subjected to sodium bisulfite conversion using the Zymo EZ DNA Methylation-Direct Kit (Zymo Research) following the manufacturer's instruction. The eluted bisulfite-converted DNA was then subject to preamplification by Klenow exo- with adaptor-marked oligos on both strands. Then, the double-tagged DNA was purified by SPRIbeads and amplified using KAPA HiFi Uracil+ ReadyMix (Kapa Biosystems) and NEBNext Multiplex Oligos for Illumina (New England BioLabs).