title: GSM6282975: Total, B1, GEX, rep1; Mus musculus; RNA-Seq
library_construction_protocol: To isolate peritoneal cells, the peritoneal wash was obtained by injecting 3-6 mL of 2% FBS with 2 mM EDTA in PBS into the peritoneal cavity. Following gentle shaking, the whole fluid was withdrawn by syringe and placed into 15 mL conical tubes. Peritoneal cells were then pelleted by centrifugation and washed with PBS. Antigen-baiting-enriched ERV-reactive B-1 cells and total B-1 cells were isolated by fluorescence-activated cell sorting (FACS). Sorted ERV-reactive B-1 cells and total naïve B-1 cells were counted and loaded into the Chromium Controller (10X Genomics) for single-cell partitioning and barcoding. Single-cell V(D)J libraries were generated using the Chromium Single Cell V(D)J Reagent kit (10X Genomics) while single-cell gene expression libraries were generated using the Chromium Single Cell 5' Reagent kit (10X Genomics), per the manufacturer's instruction.