title: Caribbean spiny lobster nervous system transcriptome
design: Total RNA was isolated from the nervous tissue using the E.Z.N.A. Total RNA Kit (Omega Bio-tek, Norcross, GA) according to the manufacturer's protocol. RNA concentration was measured using the Nanodrop 2000c spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA) and integrity was assessed using the Agilent 2200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). Next, 1 g of total RNA was used to prepare a Ribo-Zero RNA-Sequencing (Illumina, San Diego, CA, USA) library according to the manufacturer's protocol. The adapter-ligated library was PCR-amplified (15 cycles of 98 C for 10 s, 60 C for 30 s, and 72 C for 30 s), quantified, and quality-assessed using the High Sensitivity D1000 Screen Tape on the Agilent 2200 TapeStation System. Finally, Illumina indexes were added, and the library was pooled together with other libraries for multiplexed sequencing on an Illumina HiSeq4000 sequencer (Illumina, San Diego, CA, USA) using a pair-end 150 bp run format.