title: Metagenome; ID-JPL1134 Bacterial cells, Stool
design: Microbiome originating from bacteria-derived EVs and microbiome originating from bacterial cells was amplified respectively with 16S_V3_F (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3) and 16S_V4_R (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3) primers specific for V3-V4 hypervariable regions of the 16S rDNA gene233. Libraries were prepared using PCR products based on MiSeq System guide (Illumina, San Diego, CA, USA) and quantified using QIAxpert (QIAGEN, Hilden, Germany). Each amplicon was then quantified, set at an equimolar ratio, pooled, and sequenced with MiSeq (Illumina, San Diego, CA, USA), according to the manufacturers recommendations.