title: GSM6283130: donorD, CD36hiMac; Homo sapiens; RNA-Seq
library_construction_protocol: Tonsil macrophages were selected after tissue digestion by density gradient, enriched by negative selection, and isolated by cell sorting on a FACS Aria instrument. Total RNA was extracted using the Total RNA purification kit (Norgen) according to the manufacturer's protocol. cDNA was generated using the SMART-Seq v4 kit (Takara) according to the manufacturer's protocol. Libraries were prepared according to Illumina's instructions accompanying the Nextera XT DNA Library Prep Kit (Illumina). 10 ng of DNA was used for each sample. Sequencing was performed in 1 sequencing unit of NovaSeq 6000 (Illumina) (100-nt-length reads, paired end).