title: GSM5106485: ASO1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA isolated from cultured cells or under different treatment was extracted by Trizol (Invitrogen) according to the manufacturer's manual. The ribo- strand specific RNA-seq libraries were constructed by using NEBNext® rRNA Depletion Kit and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®. Briefly, total RNA were removed the ribosomal RNA from cytoplasmic and mitochondrial fraction. The ribo- RNA were reverse transcribed into cDNA and follow with second strand systhesis wiht dUTP. Then dsDNAs were ligated to adapters by end prepair with dT. PCR with specific index for sequencing.