title: GSM5899944: PMBCs for 12 individuals in pool 74; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood samples were collected in vacutainer tubes containing either FICOLLTM and sodium heparin (8mL CPTTM; BD Australia, North Ryde, NSW; 362753) or K2EDTA (10mL; BD Australia, North Ryde, NSW; Catalogue: 366643). All CPTTM tubes were processed within 4 hours of collection according to the manufacturer's recommendations. scRNA-seq libraries were prepared according to 10X Genomics Chromium v2 kit guidelines Libraries for all samples were multiplexed and sequenced across five 2‚®â150 cycle flow cells on an Illumina NovaSeq 6000.