title: GSM6249151: WT11-28-3; Mus musculus; RNA-Seq
library_construction_protocol: The RNA was amplificated according to Smart-seq2 procedures. The mRNA library was constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) according to the instruction manual, which includes sequential RNA fragmentation, reverse transcription using random primers, second strand cDNA synthesis, end repair, dA-tailing, adapter ligation, U excision, and PCR enrichment.