title: GSM5107781: TFE3-57; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from each sample using Qiagen RNeasy formalin-fixed paraffin-embedded (FFPE) Kit (Qiagen, Hilden, Germany), following the protocol from manufacturer. Purity and quantity of total RNA were measured by Nanodrop equipment. Integrity of RNA was evaluated using the RNA Nano6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). 1μg RNA of per sample was used as input for the RNA sample preparations. Strand-specific RNA sequencing libraries were generated using the Whole RNA-seq Lib Prep kit for Illumina (ABclonal, China). Library quality was evaluated on the Agilent Bioanalyzer 2100 system (Agilent, USA). Final libraries were sequenced at the Novogene Bioinformatics Institute (Beijing, China) on an Illumina Hiseq X10 platform by a 150bp paired-end reads.