title: GSM5419827: RNA-seq HrasG12V; Tgfbr2 KO (SCC) rep 2; Mus musculus; RNA-Seq
library_construction_protocol: In vivo: CHX treatment, followed by dispase/CHX treatment to separate epidermis from dermis, trypsin/CHX to obtain single cell suspension. NANOPORE long-read RNA sequencing according to the manufacturer's protocol of SQK-PCS109 (Oxford NANOPORE Technologies, UK). Ribosome profiling protocol as described in Ingolia et al., 2012. long- and short-read RNA sequencing; Ribosome profiling