title: amplicon sequencing of  the fungal ITS2 region: Carposphere BTH
design: Amplicon sequencing analysis by targeting the ITS2 region for fungi (amplified with the fITS7 and ITS4 primer pair). A two-step amplification protocol was implemented, to construct amplicon libraries. A unique 12-bp index was added to the reverse primer that served as a barcode to identify sequencing products in multiplexed libraries. 2x250 bp pair-end sequencing via the HiSeq2515 platform (Illumina, San Diego, USA) was performed at the Genome sequencing centre of the Brigham Young University (Provo, UT, USA).