title: Whole genome sequences of American mink: adult female tongue tissue
design: Genomic DNA sample meeting our quality standards is randomly fragmented by Covaris technology and the fragment with proper size obtained after fragment selection. The end repair of DNA fragments is performed and an "A" base is added at the 3'-end of each strand. Adapters are then ligated to both ends of the end repaired/dA tailed DNA fragments. For general DNBseq (DNBseq Technology) library construction, it is followed by amplification by ligation-mediated PCR (LM-PCR), while PCR-free library construction does not introduce PCR amplification at all. The next step is single strand separation and cyclization. The rolling circle amplification (RCA) is performed to produce DNA Nanoballs (DNBs). Sufficient quality DNBs are then loaded into the patterned nanoarrays and pair-end read were load on the DNBseq chip (2 x 100 bp or 2 x 150 bp).