title: GSM5420774: CRISPR_R1_high_S13; Mus musculus; RNA-Seq
library_construction_protocol: Qiagen Dneasy Blood& Tissue Kit 350bp sgRNA cassette was amplified from gDNA of virally transduced cells.  Library contains X sgRNA; therefore (1000)X reads/sample are required to maintain 1000x representation of each sgRNA.  Each sample was prepared using unique reverse primer to incorporate indexes for multiplexing. Qiagen RNA extraction protocol RNA libraries were prepared for sequencing using standard Illumina protocols