title: GSM5420479: K21_C; Hordeum vulgare; RNA-Seq
library_construction_protocol: Each sample/replicate consisted of at least 10 flag leaves, and three independent samples/replicates were harvested for both 'Karl' and '10_11' at each time point. the samples were immediately shock-frozen in liquid nitrogen and RNA was extracted separately from frozen tissues using a standard Trizol extraction method (Sigma-Aldrich). Libraries were prepared with the MARS-seq protocol (Diego Adhemar Jaitin, 2017) and sequenced using Illumina NextSeq 500 High Output v2 Kit (75 cycles).