title: GSM5425093: LXR WT-GW; Mus musculus; ChIP-Seq
library_construction_protocol: Snap-frozen mouse liver (100 mg) from wild type, Foxa2-KO, and LXRα-KO mice was used to prepare chromatin. Foxa2‐specific rabbit antiserum (Seven Hills Bioreagents, WRAB‐1,200) and rabbit polyclonal antibody specific to LXRα (Active Motif, 61175) were used for immunoprecipitation. Details about PPAR ChIP antibody: rabbit polyclonal antibody specific to PPARα (Novus Biologicals, NB600-636) Libraries were prepared according to Illumina's instructions. Briefly, DNAs were blunted with a combination of T4 DNA polymerase, Klenow polymerase, and T4 PNK, then a single 3′-end “A” base was added using Klenow exo (3′-to-5′ exo minus). multiplex adapters were then ligated to the ends of the modified DNA. After adapter ligation DNA was PCR amplified with Broad custom primers for 16 cycles and library fragments of ~280 bp (~150-bp mononucleosome insert plus ~130-bp adaptor and PCR primer sequences) were isolated using Pippin Prep agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. All libraries were sequenced on Illumina NextSeq 500.