title: GSM4828872: cebpe_bc_Rep2; Mus musculus; ChIP-Seq
library_construction_protocol: 500,000 sorted MY1+2s (pooled MY1s+MY2s), MMs, BCs, and GRs from Cebpe WT mice were used for CEBPE ChIP-seq experiments. 1,000,000 pooled MYs and MMs purified from Cebpe KO mice were used as negative control. Chromatin bound by antibodies was captured with Protein-A sepharose beads, washed, de-crosslinked and precipitated. The precipitated DNA was then mixed with 2 ng E. Coli DNA and amplified using NEB Next ChIP-seq sample prep kit (New England Biolabs) as described by the manufacturer. The indexed cDNA libraries were pooled in equimolar ratios and subjected to sequencing  on an Illumina Hiseq2000.