title: RNA-seq data of M-MSCs derived from SNUhES batch number 7
design: RNA-sequencing (R-seq) was conducted for M-MSCs. Firstly, total RNA was extracted from the M-MSCs using GeneAll Ri-bospin (GeneAll, Seoul, Korea) according to the manufacturers protocol and all RNA samples were then determined as high and comparable quality. Libraries were generated according to standard procedures using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA, USA) according to a TruSeq Stranded mRNA Sample Prep-aration Guide, Part #15031047 Rev. E (Illumina Inc.). The libraries were sequenced on a NovaSeq 6000 S4 (Illumina Inc.) according to a NovaSeq 6000 System User Guide Docu-ment #1000000019358 v02 (Illumina Inc.) in a sequencer NovaSeq (Illumina Inc.) and the set of RNA-seq data was derived from the M-MSCs. The trimmed reads scoring values of over 98% of the average Q20 and over 95% of the average Q30 in Phred quality score using BBDuk (part of BBtools) were kept. The remain-ing reads were mapped to the reference genome sequence (University of California Santa Cruz (UCSC) hg19, annotation RefSeq_2017_06_12) using Bowtie2. Calculation reads were counted using Bedtools (https://bedtools.readthedocs.io/en/latest/). Read mapping and expression quantification were performed separately for each sample.