title: GSM5553692: Tle3cKO colonic macrophages; Mus musculus; RNA-Seq
library_construction_protocol: Siglec-F(-)Ly6C(lo)CD64(+) colonic macrophages were sorted from 9-week-old male WT (four mice), Tle3cKO (three mice), and Tle4cKO (four mice) mice at the same time. Cells from mice of same genotype were pooled together. RNA was extracted using TRIzol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols by Novogene (Tianjin, China).