title: GSM4781942: D-ET6; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from fallopian tubes :early-stage fallopian tube tumors (ET), bilateral ovariectomy fallopian tubes (B) and bilateral ovarietomy DKO mice with treated P4(BP). Additionally, RNA was isolated from normal fallopian tubes (Ovid, oviducts) of respective control mice for DKO and TKO mice. Total RNA was isolated using TRIzol (Invitrogen) and purified using RNeasy MinElute Cleanup Kit (Qiagen, Germantown, MD, USA), according to manufacturer's instructions. Total RNA (1 -1.2μg) was used for mRNA library preparation. Library preparation was performed using the TruSeq Stranded mRNA HT Library preparation kit (Illumina).