title: GSM5106064: Sample 7_Aiolos_Ikzf3_WT_thymus_rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Sample 1-6: Bone marrow B220+ cells were purified using anti-CD45R coated MACS beads (Miltenyi Biotec) following manufacturer's instruction. Pre-pro B cell (B220+AA4.1+IgM-CD43+CD24-) and pre-B cell (B220+AA4.1+IgM-CD43-CD24+) were isolated by flow cytometry. Total RNA was extracted by TRIzol reagent (Thermo Fisher Scientific), followed by DNA digestion by RNA Clean & Concentrator-5 (ZYMO RESEARCH), and mRNA was obtained by poly (A) selection with mRNA DIRECT kit (Thermo Fisher Scientific). RNA-seq libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's protocol. Sample7-16: Lysates were purified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Sample 1-6: Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) Sample7-16: Libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs)