title: GSM5701121: PR8; Mus musculus; RNA-Seq
library_construction_protocol: Mock or IAV infected mice (250 PFUS) were euthanized at 7 days p.i. and whole lungs were perfused with cold PBS. Lungs were aseptically removed and placed in 1 mL complete media (10% FBS, 1% Pen/Strep in RPMI). A single-cell suspension was prepared as noted above. For CD45+ cell isolation, mock and IAV-infected lungs (n=5/group) were pooled, stained for CD45 (30-F11, BioLegend) for 1 h at RT, and sorted using the BD FACSAriaTM into 40% FBS/RPMI. After sorting, 2 x 106 cells were washed and resuspended in medium containing 10% DMSO, 20% FBS and RPMI. Cells were slowly frozen in Mr. Frosty and shipped to SingulOmics (NYC, NY). RNA libraries were prepared with the Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.