title: GSM4783534: MSC_1_Zymosan; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated by column separation using a RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), according to manufacturer's protocol. RNA quality was assessed with Bioanalyzer2100 RNA Nano 6000 chips (Agilent technologies, Santa Clara, California, USA) and samples with RIN > 9 were used for sequencing. An Illumina sequencing library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, MA, USA) following manufacturer's protocols.