title: GSM5106734: AA0483 (SP-06); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was performed using the Allprep DNA RNA Protein Minikit from Quiagen following manufacter instructions. Starting material included three times PBS washed cellular pellets of 106 cells. DNA and RNA concentration were determined on a NanoDrop ND-3300 Fluorospectrometer, and RNA integrity was examined with the Bioanalyzer 2100 (Agilent). RNA sequencing (RNA-seq) was performed at the Centro Nacional de Análisis Genómico (CNAG) in Barcelona, Catalonia. The RNASeq libraries were prepared from total RNA using the TruSeq™ RNA Sample Prep Kit v2 (Illumina Inc.,) with minor modifications.