title: GSM5425339: Healthy Donor 2; Homo sapiens; RNA-Seq
library_construction_protocol: BOECs from 2 PCV and 2 normal lines were seeded and subjected to orbital flow as described in Orbital Flow Setup. After 24h of orbital flow, the BOECs were trypsinized, resuspended in heparin-free, EGM-2 medium (10% heat-inactivated FBS) and counted using Trypan blue and an automated cell counter (Countess II, Thermo Fisher Scientific) and resuspended appropriately for loading onto 10X Genomics Chromium Controller chip by facility personnel at Single-cell Omics Centre (SCOC), Genome Institute Singapore (GIS). Each BOEC cell line was prepared as a separate scRNA-seq library using Chromium Single Cell 3' v3 Reagent Kit (10X Genomics) by SCOC GIS and the final ready-to-sequence libraries were handed over with quantification and quality assessment reports from Bioanalyzer Agilent 2100 using the High Sensitivity DNA chip (Agilent Genomics). Individual libraries were pooled equimolarly and sent for sequencing by NovogeneAIT Genomics (Singapore).