title: GSM5599633: LITC x High-spine Embryo2_9 Rep2; Gasterosteus aculeatus; RNA-Seq
library_construction_protocol: Embryos were flash frozen in liquid nitrogen at 72 hours post fertilization and stored at -80 degrees. The tissues were homogenized in a MP FastPrep with Matrix A. RNA was extracted with Qiagen All-prep DNA/RNA kit. 200 ng of total RNA was used to make sequencing libraries with Illumina Stranded mRNA Prep kit according to manufacturer's instructions. 12 PCR cycle were done to generate the libraries.