title: GSM5107556: SKIN_EBF2+_REP1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from freshly sorted skin Ebf2+ cells, skin Ebf2- PαS cells and BM MSC were isolated with RNeasy Micro Kit (Qiagen) according to the manufacturer's protocol. cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio. Cat. No. 634898). The cDNA quality was examined on Agilent TapeStation system using a High Sensitivity D5000 ScreenTape (Agilent, Cat. No. 5067-5592). One ng cDNA was used for library preparation using Nextera XT DNA Library Preparation Kit (Illumina, Cat. Nos. FC-131-1024 & FC-131-1096). ). The yield and quality of the amplified libraries were analyzed using Qubit by Thermo Fisher and the Agilent Tapestation System. The indexed cDNA libraries were sequenced on the Illumina 2000 or Nextseq 550 (Illumina, San Diego, CA) for a 75-cycle v2 sequencing run generating 75 bp single-end reads.