title: DNA seq of wild germplasm
design: Total genomic DNA was isolated from fresh leaves of a single plant of each genotype using a modified cetyl trimethyl ammonium bromide (CTAB) method. The concentration and quality was monitored on NanoDrop8000 Spectrophotometer. Genotyping by sequencing (GBS) of DNA samples was outsourced by ddRAD method utilizing two restriction enzyme system from Illumina HiSeq2000 platform.