title: GSM5466108: gz_iPS_cloneA_p31_rep3; Equus grevyi; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNeasy Mini Kit 50 (Qiagen) according to the manufacturer's instructions. DNA was eliminated with RNase-Free DNase Set (Qiagen) in solution followed by RNA cleanup. The RNA solution was quantified using the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA (40 ng) was used for library preparation for Oxford Nanopore Technologies (ONT) long-read cDNA sequencing. The cDNA was generated using the PCR-cDNA Barcoding kit (SQK-PCB109) by ONT according to the manufactures protocol. For sequencing, libraries were applied to the Nanopore Flow Cell (v 9.4.1) and ran for up to 72 h.