title: GSM5106136: MITF bio rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: Overexpression and knockdown were confirmed using western blot prior to RNA isolation.  Once validated, RNA was extracted from these cells using an RNA extraction kit from Qiagen. Sample quality was confirmed using a Nanodrop and QC analysis was performed using a bioanalyzer to obtain RIN values.  Samples with appropriate RIN values were then chosen such that we had three independent triplicate samples for each condition. RNA library preparation was performed using a Tru Seq stranded total RNA kit from Illumina. The library pool was diluted and denatured according to the standard NextSeq protocol (Document # 15048776 v02). Sequencing was performed using the Illumina NextSeq 500 (NextSeq control software v2.1.0 / Real Time Analysis v2.4.11) to generate paired-end 76 bp reads using a 150 cycle NextSeq500/550 High Output reagent Kit v2 (illumina, FC-404-2002). Sequencing was performed at the Institute for Molecular Bioscience Sequencing Facility (University of Queensland).