title: GSM6281484: A375_Clone1_Bottom5_HiC; Homo sapiens; Hi-C
library_construction_protocol: 5 million cells of each Clonal condition were processed using the Arima-HiC+ Kit (Mammalian Cell Lines A160134v01) protocol. 5 MDA-MB-231 subpopulation cells were fixed with 1% formaldehyde, suspended in cell lysis buffer for permeabilization, and homogenized by douncing. Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation, adaptor ligation, and PCR amplification steps were carried out on bead bound DNA libraries.