title: genome sequencing of Cerrena unicolor SP02
design: Sequencing library preparations were constructed following the manufacturers protocol. 100 ng genomic DNA was randomly fragmented to <500 bp. The fragments were treated with End Prep Enzyme Mix, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was performed, and then fragments of ~470 bp were recovered. Each sample was then amplified by PCR for 8 cycles using P5 and P7 primers. The PCR products were cleaned up and validated. Then libraries with different indices were multiplexed and loaded on an Illumina HiSeq.