title: RIP-H9-MSI1FL-Flag
design: RNA immunoprecipitation was performed with reversible cross-linking of the cells, as previously described (Niranjanakumari et al., 2002). The RNA was extracted by using Trizol reagent (Invitrogen). RNA quality was determined and use to construct sequencing library. libraries were prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA), cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illumina protocol. Libraries were then size selected for cDNA target fragments  on 2% Low Range Ultra Agarose (Sigma-Aldrich) followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantification, paired-end libraries were sequenced by Illumina NovaSeq 6000 sequencing.