title: GSM5553872: ChIP-seq_HSF1_MEF_C_Rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were washed with PBS after treatments. Next, cells were lysed and chromatin was fragmented with sonicator (Bioruptor Pico). Protein-DNA complexes were isolated using protein G sepharose beads and antibodies against HSF1 (ADI-SPA-901, Enzo) and HSF2 (Östling et al., J. Biol. Chem. 282, 7077-7086). Normal rabbit IgG was used as a negative control. Chromatin extracted from heat-shocked human Hs578T cells was used as a spike-in for ChIP-seq. Libraries were constructed using NEXTflex ChIP-seq kit (Perkin Elmer) and NEXTflex ChIP-seq barcodes (Perkin Elmer).