title: GSM6283572: SHE-BT-s003-1; Mus musculus; RNA-Seq
library_construction_protocol: scRNAseq libraries were generated using the SORT-seq protocol. Single cells were lysed at 65ºC for 5 min,and second-strand mixers and reverse transcriptase were then added to the wells using the Nanodrop II liquid handling platform (GC Biotech). After reverse-transcribing the mRNA of each cell, double-stranded cDNAs from single cells were pooled and in vitro transcription was performed for linear amplification, which resulted in amplified RNA. TruSeq small RNA primers (Illumina) were used to prepare the Illumina sequencing libraries and these DNA libraries were then sequenced paired-end at 75 bp read length using the Illumina NextSeq