title: GSM5107379: RNA-seq_Non-silenced control rep2; Homo sapiens; RNA-Seq
library_construction_protocol: All cell types were plated in triplicate at 1 X 10^6 cells/well in 6-well culture plates.  Cells were trypsinized and counted.  16,667 cells from each cell type's 3 wells were combined to have 50,000 cells per cell type for DNA extraction for ATAC-Seq.  The remaining cells underwent RNA extraction using the ReliaPrep RNA Cell Miniprep System (Promega; catalog #Z6012).  Cells for ATAC-Seq were lysed, and the Nextera DNA Library Prep Kit was used for transposition.  DNA was purified using the Qiagen MinElute Reaction Cleanup Kit.  PCR Amplification was performed using the NEBNext High-Fidelity 2x PCR Master Mix.  AMPure XP beads were used for library purification. RNA libraries were prepared for sequencing using standard Illumina protocols