title: GSM5105201: Female control at 20:00, replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using a Direct-zol-96 kit per the manufacturer's instructions (Zymo Research). RIN values were assessed via the Agilent TapeStation. Library prep was completed using poly(A) selection with a TruSeq stranded mRNA kit (Illumina) to remove rRNA and samples were barcoded using unique dual indexing before the samples were again assessed on the TapeStation to check the length distribution of the libraries. Paired end sequencing (150bp) was conducted on a HiSeq 4000 (Illumina) to generate approximately 312 million read pairs.