title: RNA sequencing revealed similar reduction in muscular gene expression profiles of methyltrasferases when fed methionine enriched diets supplemented with either threonine, lysine and taurine or cobalamine, pyridoxine and folate during the freshwater period of Atlantic salmon
design: RNA-sequencing (RNA-seq) was performed by the DeepSeq sequencing facility at Nord University, Bod, Norway using the muscle RNA used for RT-qPCR . Eighteen cDNA RNA- Seq libraries for sequencing were constructed using 1 g of total RNA per sample and completed using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) with Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA), according the manufacturers protocol. Samples were multiplexed into two pools of nine samples, with multiplexing barcodes ligated to each sample during the PCR amplification step. The final libraries were quantified, normalized, pooled at equimolar ratios, and sequenced on the Illumina NextSeq 500 sequencer (Illumina, USA) with 75 base pairs single-end reads.