title: immature seeds of Zhongzhi16 at 10 DPA
design: The mRNA was isolated by using Oligo (dT) and then mixed with the fragmentation buffer. Next, the cDNA was synthesized using the mRNA fragments as templates. Short fragments were purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments were ligated with adapters