title: GSM5795706: siNC_2; Homo sapiens; RIP-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Micro Kit (Cat#74004, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set  (Cat#79254, QIAGEN, GmBH, Germany) We utilized input and post m6A-IP positive fraction for library construction utilizing the Illumina TrueSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.