title: GSM5699895: E12.5 in vivo_A8; Ovis aries; RNA-Seq
library_construction_protocol: Embryos were snap-frozen in liquid nitrogen, and total RNA was extracted using MagMAXTM mirVanaTM Total RNA Isolation kit according to the manufacturer´s protocol. cDNA was synthetized with SMART-Seq™ v4 Ultra™ Low Input RNA Kit (Clontech) and amplified ds-cDNA was purified with AMPure XP beads (Beckman Coulter) and quantified with Qubit (Life Technologies). Libraries were prepared using Covaris shearing system and a size selection of 200 bp was performed. RNA libraries were prepared for sequencing using standard Illumina protocols