title: RNA-Seq of tomato dry seed
design: RNA was isolated using the NucleoSpin RNA plant isolation kit (Macherey-Nagel 740949) with on-column DNA digestion and adding Plant RNA isolation Aid (Life technologies) according to the manufacturers protocol and instructions. Strand-specific RNA-seq libraries were prepared from each RNA sample using the TruSeq RNA kit from Illumina according to manufacturers instructions. Poly-A-selected mRNA was sequenced using the Illumina HiSeq2500 sequencer, producing strand-specific single-end reads of 100 nucleotides.