title: GSM5652170: BT-20-SAMD9-R1293W-with-inducer-S4; Homo sapiens; RNA-Seq
library_construction_protocol: Total cellular RNA was extracted with RNeasy plus mini kit (Qiagen). 5 μg of the total RNA was used for isolation of mRNA with the Poly(A) mRNA magnetic isolation module (NEB). The mRNA was heat fragmented with RNA Fragmentation Reagents (Thermo Fisher Scientific). cDNA library was constructed with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB).