title: GSM5420710: SP_wt_R1; Schizosaccharomyces pombe; OTHER
library_construction_protocol: Nuclear run-on reactions and RNA extractions were performed based on the published protocol (Mahat et al., 2016) with minor modifications according to the previous study (Booth et al., 2018; Hou et al., 2019). Library was generated followed by the published PRO-seq protocol (Mahat et al., 2016) from RNA fragmentation by base hydrolysis to full-scale PCR amplification with few differences of reagent. DNA libraries of ~100bp to 350bp were selected by agarose gel extraction (Zymo Research, D4007) according to the manufacturer's protocol.