title: Whole genome sequences(HiFi reads) of American mink: adult female tongue tissue
design: The gDNA size and integrity were verified by pulsed field gel electrophoresis (PFGE) before beginning library preparation. The gDNA was sheared to achieve a specified target fragment size and the sheared gDNA was concentrated using AMPure PB Beads. Then, the single-strand overhangs were removed, and the DNA damage was repaired. End-repair/A-tailing and adapter ligation were performed at the next steps. The SMRTbell templates were purified using AMPure PB beads and the size selection was performed using the BluePippin System. The SMRTbell templates were again purified after size selection. Finally, primer annealing and polymerase binding were set for SMRTbell templates and libraries were prepared for sequencing.