title: GSM6283034: Mock2; Mus musculus; ssRNA-seq
library_construction_protocol: Hippocampus were homogenized TRIzol (1 mL per 100 mg of tissue) and incubated for 24 hours. Samples were then incubated in chloroform for 5 min, then centrifuged at 12,000 g for 15 min at 4 ̊C. The aqueous upper phase containing RNA was transferred to a tube containing an equal volume of 70% ethanol and vortexed. RNA was treated with DNases and isolated using the PureLink RNA isolation kit (ThermoFisher Scientific Cat# 12183025) To generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). Samples were processed following the manufacturer's instructions, modifying RNA shear time to five min. Resulting libraries were multiplexed and sequenced with 75 basepair (bp) single reads (SR75) to a depth of approximately 25 million reads per sample on an Illumina HiSeq4000