title: GSM5105590: 3xFLAG-dSpCas9 rep 1 FLAG IP; Homo sapiens; RIP-Seq
library_construction_protocol: Biological replicates of confluent 10cm plates of 293T cells were treated with 400mJ/cm2 of UV twice using the Stratalinker 2400, harvested in ice cold PBS and pellets flash frozen in liquid nitrogen and stored in -80 degrees until ready to IP in a subsequent protocol exactly as detailed in Van Nostrand et al (2016), cutting the nitrocellulose membrane from 115 kDa and up. Sequencing was performed on Illumina HiSeq 4000 with paired end reads and data processed through Dr. Yeo's eCLIP pipeline. Libraries were constructed as per the eCLIP protocol using Illumina 50x forward and 70x reverse primers.