title: GSM5701079: OVISE_Final_rep1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted using a QIAGEN Blood and Cell Culture DNA Midi Kit (#13343) according to the manufacturer's protocol. 125 μg of genomic DNA from each sample was split into 2.5 μg fractions and sgRNA sequences were amplified using Herculase II enzyme following published protocols and instructions supplied by authors on Addgene #90294 (Hart T et al., G3 (Bethesda) 2017. 7(8): 2719-2727). Reactions were measured for fragment size using the Agilent 2200 Tapestation, and quantified using the KAPA SYBR Fast qPCR Kit (KAPA Biosystems, #7959362001).