title: GSM6281560: DMSO1_complete; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from the cell pellets either from sorting or pooled cells using the GeneJET Genomic DNA Purification Kit (Thermo Scientific™, Cat. No K0721). PCR was performed with NEBNext® High-Fidelity 2X PCR Master Mix (M0541). Thermal cycler parameters were set to: initial denaturation for 5 min at 98°C, 20 cycles of denaturation at 98°C for 30 s, annealing for 30 s at 58°C, extension for 40 s at 72°C, and final extension for 5 min at 72°C. PCR products were purified via 1% agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, 28706). All samples were denatured and diluted according to the Illumina NextSeq system denature and dilute libraries guide (document # 15048776 v09, illumina.com) and sequenced on an Illumina NextSeq 500. Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437