title: Illumina HiSeq 2500 paired end sequencing; PRMT5 genomic deregulation supports growth and stress tolerance in chronic lymphocytic leukemia
design: PRMT5 genomic deregulation supports growth and stress tolerance in chronic lymphocytic leukemia
library_construction_protocol: Cell lines were taken from the stock of the cell lines bank (Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures). Cell lines were authenticated by DNA profiling and cytogenetics. HG-3 and PGA-1 cells were treated with 10 ÂµM EPZ015666 for 96 h or incubated with a similar volume of DMSO as control, prior to isolation of total RNA for sequencing Total RNA was extracted via miRNeasy Mini Kit (Qiagen, Hilden, Germany) including DNase digestion. Strand-specific (fr-first strand) mRNA libraries were produced, quality controlled via Applied Biosystems Fragment fluorometer.