title: GSM5420923: KO_GSK862_H3K27ac; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with formaldehyde followed by neutralization. Cells were then harvested followed, cell lysis. Lysates were sonicated, and clarified by centrifugation. Sonicated nuclei and histone-DNA complexes were isolated with antibody of interest.  Antibody bound DNA with magnetic beads were washed, and DNA was eluted followed by reverse cross-linking, and DNA purification. DNA fragments range from 200-600 bp were size-selected for subsequent ChIP-seq  library preparation with TruSeq ChIP Sample Prep Kit (Illumina) according to manufacturer's instructions.  Sequencing libraries were multiplexed and ran on a Hiseq2500 platform