title: RNA-Seq of rice germinated seeds:second stage under low temperature-3
design: For library construction,total RNA was isolated from the seeds at second germinating stage under low temperature using EASYspin Plus Complex Plant RNA Kit (Aidelai, China) according to the manufacturers instructions. 1.5 g of total RNA from each sample was used for rRNA removal using the Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA). Sequencing libraries were generated using NEBNextR UltraTM Directional RNA Library Prep Kit for IlluminaR (NEB, USA) following manufacturers instructions.RNA libraries were prepared for sequencing using standard Illumina protocols.