title: GSM5230643: VDR-/-_PBS_Nucleus Accumbens [9A]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using a Trizol based protocol. The extracted RNA samples where checked for overall quality and only samples with high quality (RIN ≥ 8.0) and high purity (OD 260/280 = 1.8–2.0) were used to generate libraries using the Bioo Scientific NEXTflex Rapid Directional RNA-seq Library Prep Kit (Bioo Scientific, Auston TX). The final library was quantitated using the Agilent 2200 TapeStation High Sensitivity DNA Analysis (Agilent Technologies, Santa Clara, CA) for size and Qubit dsDNA HS Assay (Life Technologies, Waltham, MA) for concentration. The resulting mRNA libraries were then sequenced at the John Wayne Cancer Institute Sequencing Center on an Illumina HiSeq 2500 in Rapid Mode using 101 bp paired-end reads. RNA-seq libraries were constructed using the Bioo Scientific NEXTflex Rapid Directional RNA-seq Library Prep Kit (Bioo Scientific, Auston TX) following the manufactures protocol.