title: Amplicon sequencing of the bacterial 16S rRNA gene for venous stasis ulcer microbiome
design: Genomic DNA was extracted from 22 swab samples using the DNeasy PowerWater Kit (Qiagen) and normalized on an Eppendorf epMotion 5075 Liquid Handling Workstation to 0.2 ng/mL. Primers were designed to target the 16S V3 and V4 regions (forward primer: 5-TCGTCGGCAGCG