title: Seawater of Outfall Area 2 (OA2) collected during March-2017
design: To understand the response of marine bacterioplankton community in future climate changes such as global warming, surface seawater were sampled from the control (unimpacted) and thermal discharge-impacted areas of a power plant, located at the southern coastal region of India. Sampling was performed consecutively for one year (September 2016 to August 2017) on monthly basis. The collected surface seawater samples were subjected to total community DNA extraction using PowerWater DNA Isolation Kit (MoBio Laboratories Inc., USA) following the manufacturer's guidelines and the hypervariable V3-V4 regions of 16S rRNA gene was amplified using universal bacterial primer pair tagged with unique barcode sequences. The size and quantity of prepared library was validated by running on Bioanalyzer 2100 (Agilent Technologies, California, US). The equimolar concentrations of prepared libraries were taken and then tag sequenced on the Illumina MiSeq sequencer using 300-bp paired-end method at Macrogen Inc. (Seoul, Korea).