title: GSM5107708: IN54-Cas9-gRNA-cas9-3; Homo sapiens; OTHER
library_construction_protocol: The cells were trypsinized and collected, and genomic DNA was extracted using the TIANamp Genomic DNA Kit (TIANGEN) Amplification primers containing Illumina forward and reverse adapters were used for a first round of PCR (PCR 1) amplifying the genomic region of interest. Unique Illumina barcoding primer pairs were  added to each sample in a secondary PCR reaction (PCR 2).