title: GSM5466868: CD11c+CD11b+ (rep 1); Mus musculus; RNA-Seq
library_construction_protocol: Skin was minced and incubated in 2 mg/mL Collagenase Type IV (Worthington Biochemical Corporation), 0.2 mg/mL DNase I (Sigma-Aldrich), and 2% FBS in HBSS at 37°C for 25 minutes with a magnetic stir bar. Remaining skin fragments were mechanically dissociated in RPMI-1640 containing 10% FBS and 2mM EDTA. 2 mice were pooled prior to staining, for a total of 3 experimental samples. Single cell suspensions were prepared for each tissue and stained with anti-mouse CD8a-PerCP/Cynine5.5 (clone 53-6.7; Biolegend; #100773), anti-mouse CD11c-Alexa Fluor 647 (clone N418; Biolegend; #117314), anti-mouse CD11b-Alexa Fluor 488 (clone M1/70; Biolegend; #101219), anti-mouse CD45.2- Alexa Fluor 488 (clone 104; Biolegend; #109821). Libraries were generated from 10ng cDNA using the Nextera DNA Flex library prep kit (Illumina). Libraries underwent quality control by Fragment Analyzer (Agilent) and Qubit (ThermoFisher) to determine the size distribution and the quantity of the libraries. Libraries were sequenced on a NextSeq 500 (Illumina).