title: GSM6281604: YE3-2; Oryza sativa; RNA-Seq
library_construction_protocol: total RNAs were extracted with RNAprep Pure Plant Kit (TIANGEN, Beijing, China) and quantified by NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific) For cDNA synthesis, a total 1 μg RNA for each sample was treated with DNase I to eradicate the genomic DNA and then used as a template for reverse transcription (QuantiTect Reverse Transcription Kit, Qiagen, China). We added fragment buffer to break into short segments using short segment RNA as template. Sequencing libraries was created using NEB Next Ultra RNA Library Prep Kit following manufacturer′s instructions. The index codes were added to each sample. Paired-end cDNA libraries with an insert size of 300 bp were constructed for transcriptome sequencing.