title: GSM4827369: MDF_KO_Lap2alpha_15FBS; Mus musculus; ChIP-Seq
library_construction_protocol: Ready cells were fixed in 1% paraformaldehyde/PBS for 10 min at RT, crosslinking was stopped by adding cold glycine to a final concentration of 0.125 M for five min, followed by harvesting in 500 ml of ice-cold PBS and spinning at 1000g for 5 min at +4°C. Cell pellets were lysed in 300 µl of RIPA buffer and sonicated with Bioruptor (Diagenode; number of cycles = 15, power = HIGH, ON = 30 sec, OFF = 30 sec). IPs were carried out with 5 µg antibody overnight at 4°C in a rotating wheel. ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer's protocols.