title: GSM5421284: 188_jejunum_PN-sens_PN_30min; Mus musculus; RNA-Seq
library_construction_protocol: Murine jejuna (starting 6 cm from pyloric junction and continuing for 4 cm) were obtained by sterile dissection, flushed with cold sterile PBS, and cut open longitudinally. Tissue was incubated in Isolation Buffer with freshly added 2 mM EDTA and 0.5 mM DTT at room temperature for 75 minutes, washed in PBS twice, and shaken vigorously in Isolation Buffer to release the epithelial cells. Preparation of RNA library and transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China).