title: GSM5467456: RHCD_AR006_GCCAAT_L006_R1_goodreads_st10trns1MOa; Xenopus tropicalis; RNA-Seq
library_construction_protocol: RNA from individual X. tropicalis embryos was isolated using the Trizol Reagent (Ambion, 15596026), optimized for extracting small amounts of RNA from single embryos. RNA-seq libraries were made following the Low Sample (LS) Protocol from the TruSeq RNA Sample Preparation v2 Guide. The important modification made to this protocol was that all reagents, except for the Bead Washing Buffer, were used at half volume. Final RNA-seq libraries were quantified using the KAPA Library Quantification Kits for Illumina sequencing platforms (Roche, KK4824).