title: GSM4781663: Input_GS; Mus musculus; ChIP-Seq
library_construction_protocol: For ChIP experiments, cell lysates were clarified from sonicated nuclei and IP with mePontin antibody. The sequencing library is prepared by random fragmentation of the DNA, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.