title: GSM5701279: HeLa-cas9 cells 'Bottom 20%' ataxin-2 with GAPDH control 1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted for all screen populations separately according to the protocol included with QIAGEN Blood Maxi Kit. sgRNA sequences were amplified and prepared for deep-sequencing by two sequential PCR reactions as described previously (Morgens et al., 2016).