title: GSM5466035: Ctrl_1; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA, including small RNAs from two groups of control and RANBP1-knockdown HCT116 cells, were extracted using the Total RNA Purification Kit from Norgen Biotek Corp (Thorold, Canada). Approximately 1 μg total RNA was used to prepare a small RNA library according to the protocol of the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA). Then, single-end sequencing (36 bp or 50 bp) was performed on the Illumina HiSeq 2500 System (LC Sciences, Houston, TX) according to the manufacturer's recommended protocol. MicroRNA sequencing Total RNA, including small RNAs, was extracted using a commercial extraction kit from Total RNA Purification Kit from Norgen Biotek Corp (Thorold, ON, Canada) in cultured mCECs in the SCIP assay. RNA quality and quantity were analyzed using a Bioanalyzer 2100 (Agilent, CA, USA) to ensure a RIN number > 7.0.