title: GSM5397031: 6C; Homo sapiens; RNA-Seq
library_construction_protocol: Whole blood was treated with ACK lysis buffer for 15 minutes at RT to remove red blood cells. Cells were resuspended in Trizol (300µL) and RNA was extracted using Zymo's Direct-zol extraction kit as per the manufacturer's instructions. Sequencing libraries were prepared from the eluted RNA using the Ovation Ultralow V2 DNA-Seq Library Preparation Kit following the manufacturer's instruction (NuGEN) at the Mount Sinai Oncological Sciences Sequencing Facility. Sequencing was performed on an Illumina NextSeq 500 instrument to produce 75-bp single-end reads.