title: GSM5733424: Mouse kidney scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: For each sample mentioned above, nuclei were extracted from the kidney tissue, fixed and snap-frozen. We generated a sci-RNA-seq3 (current version of sci-RNA-seq) library based on a previously demonstrated protocol, including nuclei permeabilization, three-level combinatorial indexing composed of reverse transcription (RT), hairpin ligation and indexed PCR, and library purification. Libraries were preparedusing the current protocol of sci-RNA-seq3.