title: GSM5106791: Pax7 WT FI-SC replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Freshly isolated satellite cells (α7-INTEGRIN+, CD34+, CD31-, CD11b-, SCA-1- and CD45-) were sorted from uninjured hindlimb muscles,  using a MoFlo XDP cell sorter (Beckman Coulter). Total RNA was purified using Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) using the manufacturer's instructions. For each biological sample, 15ng of RNA was used as input for the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs).