title: GSM5701177: HeLa SMN CRISPR mutant clone 2  (5A); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with Trizol reagent (Ambion), treated with Ambion™ DNase I (RNase-free) extracted with phenol/chloroform. RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent technologies, Santa Clara, CA) TruSeq Stranded mRNA kit (Illumina, San Diego, CA) has been used for library preparation following the manufacturer's instructions (library type: fr-firstrand). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay.