title: GSM5652417: h0_NT30_NoInfection Sample30_EpiTime_0_DonorNA_NoInfection_S34; Homo sapiens; RNA-Seq
library_construction_protocol: Filters containing epithelial cells were incubated with Trizol. RNA was extracted following standard chloroform extraction followed by a cleanup with sodium acetate precipitation. RNA quality and concentration were quantified using a Bioanalyzer (Agilent technologies, Inc.) and only samples with RIN>8.0 were used for sequencing. Libraries were prepared using the TruSeq RNA Single Indexes Set B kit (Illumina) and AmPure beads (Beckman) and sequenced as paired end (100bp) by high seq rapid run (Illumina) with an aim of ~20 million reads per sample