title: GSM5420289: RNA Pol. II HiChIP Auxin- rep1; Homo sapiens; OTHER
library_construction_protocol: Fixed chromatin was digested using DpnⅡ enzyme, followed by biotin incorporation with Biotin-14-dATP. After DNA ligation, DNA was sonicated and immunoprecipitated with antibody. Reverse-crosslinked DNA was captured with Streptavidin C-1 beads. Libraries were prepared using Nextera DNA library preparation kit according to manufacturer's instructions. DNA was amplified using Nextera PCR primers. Libraries were purified using SPRI AMPureXP beads and were paired-end sequenced on Illumina HiSeq2500 platform hic contaning validpairs